LOCUS       DAJTMK010000001        69765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTMK010000001 DAJTMK010000000
VERSION     DAJTMK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69765
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.001"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..258
                     /locus_tag="OO135_000001"
     CDS             <1..258
                     /locus_tag="OO135_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5395997.1"
                     /translation="GLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSV
                     VMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            268..1642
                     /gene="gspE"
                     /locus_tag="OO135_000002"
                     /pseudo
     CDS             268..1642
                     /gene="gspE"
                     /locus_tag="OO135_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1632..2818
                     /gene="hofC"
                     /locus_tag="OO135_000003"
                     /pseudo
     CDS             1632..2818
                     /gene="hofC"
                     /locus_tag="OO135_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(2853..3896)
                     /gene="guaC"
                     /locus_tag="OO135_000004"
     CDS             complement(2853..3896)
                     /gene="guaC"
                     /locus_tag="OO135_000004"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5395998.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGTDIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4052..4096)
                     /locus_tag="OO135_000005"
     CDS             complement(4052..4096)
                     /locus_tag="OO135_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5395999.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4121..4741
                     /gene="coaE"
                     /locus_tag="OO135_000006"
     CDS             4121..4741
                     /gene="coaE"
                     /locus_tag="OO135_000006"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5396000.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4741..5484
                     /gene="zapD"
                     /locus_tag="OO135_000007"
     CDS             4741..5484
                     /gene="zapD"
                     /locus_tag="OO135_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5396001.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5494..5691
                     /gene="yacG"
                     /locus_tag="OO135_000008"
     CDS             5494..5691
                     /gene="yacG"
                     /locus_tag="OO135_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5396002.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5798..6187)
                     /gene="mutT"
                     /locus_tag="OO135_000009"
     CDS             complement(5798..6187)
                     /gene="mutT"
                     /locus_tag="OO135_000009"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5396003.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6247..8952)
                     /gene="secA"
                     /locus_tag="OO135_000010"
     CDS             complement(6247..8952)
                     /gene="secA"
                     /locus_tag="OO135_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5396004.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9014..9526)
                     /gene="secM"
                     /locus_tag="OO135_000011"
     CDS             complement(9014..9526)
                     /gene="secM"
                     /locus_tag="OO135_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5396005.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9756..10673)
                     /gene="lpxC"
                     /locus_tag="OO135_000012"
     CDS             complement(9756..10673)
                     /gene="lpxC"
                     /locus_tag="OO135_000012"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5396006.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10774..11925)
                     /gene="ftsZ"
                     /locus_tag="OO135_000013"
     CDS             complement(10774..11925)
                     /gene="ftsZ"
                     /locus_tag="OO135_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5396007.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(11986..13248)
                     /gene="ftsA"
                     /locus_tag="OO135_000014"
     CDS             complement(11986..13248)
                     /gene="ftsA"
                     /locus_tag="OO135_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5396008.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13245..14075)
                     /gene="ftsQ"
                     /locus_tag="OO135_000015"
     CDS             complement(13245..14075)
                     /gene="ftsQ"
                     /locus_tag="OO135_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5396009.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14077..14997)
                     /gene="ddlB"
                     /locus_tag="OO135_000016"
     CDS             complement(14077..14997)
                     /gene="ddlB"
                     /locus_tag="OO135_000016"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5396010.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(14990..16465)
                     /gene="murC"
                     /locus_tag="OO135_000017"
     CDS             complement(14990..16465)
                     /gene="murC"
                     /locus_tag="OO135_000017"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5396011.1"
                     /translation="MNTQQLAKLRTIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16519..17586)
                     /gene="murG"
                     /locus_tag="OO135_000018"
     CDS             complement(16519..17586)
                     /gene="murG"
                     /locus_tag="OO135_000018"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5396012.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17583..18827)
                     /gene="ftsW"
                     /locus_tag="OO135_000019"
     CDS             complement(17583..18827)
                     /gene="ftsW"
                     /locus_tag="OO135_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5396013.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18827..20143)
                     /gene="murD"
                     /locus_tag="OO135_000020"
     CDS             complement(18827..20143)
                     /gene="murD"
                     /locus_tag="OO135_000020"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5396014.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20146..21228)
                     /gene="mraY"
                     /locus_tag="OO135_000021"
     CDS             complement(20146..21228)
                     /gene="mraY"
                     /locus_tag="OO135_000021"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5396015.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21222..22580)
                     /gene="murF"
                     /locus_tag="OO135_000022"
     CDS             complement(21222..22580)
                     /gene="murF"
                     /locus_tag="OO135_000022"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5396016.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22577..24064)
                     /gene="murE"
                     /locus_tag="OO135_000023"
     CDS             complement(22577..24064)
                     /gene="murE"
                     /locus_tag="OO135_000023"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5396017.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQYRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINLNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24051..25817)
                     /gene="ftsI"
                     /locus_tag="OO135_000024"
     CDS             complement(24051..25817)
                     /gene="ftsI"
                     /locus_tag="OO135_000024"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5396018.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGSDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25833..26198)
                     /gene="ftsL"
                     /locus_tag="OO135_000025"
     CDS             complement(25833..26198)
                     /gene="ftsL"
                     /locus_tag="OO135_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5396019.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26195..27136)
                     /gene="rsmH"
                     /locus_tag="OO135_000026"
     CDS             complement(26195..27136)
                     /gene="rsmH"
                     /locus_tag="OO135_000026"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5396020.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIACAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27138..27596)
                     /gene="mraZ"
                     /locus_tag="OO135_000027"
     CDS             complement(27138..27596)
                     /gene="mraZ"
                     /locus_tag="OO135_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5396021.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEVIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGNLSERLQDLSL"
     gene            complement(28198..29202)
                     /gene="cra"
                     /locus_tag="OO135_000028"
     CDS             complement(28198..29202)
                     /gene="cra"
                     /locus_tag="OO135_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5396022.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29284..29370)
                     /locus_tag="OO135_000029"
     CDS             complement(29284..29370)
                     /locus_tag="OO135_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5396023.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29382..29873)
                     /gene="ilvN"
                     /locus_tag="OO135_000030"
     CDS             complement(29382..29873)
                     /gene="ilvN"
                     /locus_tag="OO135_000030"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5396024.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLALIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29876..31600)
                     /gene="ilvI"
                     /locus_tag="OO135_000031"
     CDS             complement(29876..31600)
                     /gene="ilvI"
                     /locus_tag="OO135_000031"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5396025.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31719..31763
                     /locus_tag="OO135_000032"
     CDS             31719..31763
                     /locus_tag="OO135_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396026.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31918..32862)
                     /gene="leuO"
                     /locus_tag="OO135_000033"
     CDS             complement(31918..32862)
                     /gene="leuO"
                     /locus_tag="OO135_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5396027.1"
                     /translation="MPEVQTDHSETVELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            33523..33609
                     /gene="leuL"
                     /locus_tag="OO135_000034"
     CDS             33523..33609
                     /gene="leuL"
                     /locus_tag="OO135_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5396028.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            33702..35273
                     /gene="leuA"
                     /locus_tag="OO135_000035"
     CDS             33702..35273
                     /gene="leuA"
                     /locus_tag="OO135_000035"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5396029.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDVVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            35273..36364
                     /gene="leuB"
                     /locus_tag="OO135_000036"
     CDS             35273..36364
                     /gene="leuB"
                     /locus_tag="OO135_000036"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5396030.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAAYAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            36367..37767
                     /gene="leuC"
                     /locus_tag="OO135_000037"
     CDS             36367..37767
                     /gene="leuC"
                     /locus_tag="OO135_000037"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5396031.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEVATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            37778..38383
                     /gene="leuD"
                     /locus_tag="OO135_000038"
     CDS             37778..38383
                     /gene="leuD"
                     /locus_tag="OO135_000038"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5396032.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(38432..39514)
                     /locus_tag="OO135_000039"
     CDS             complement(38432..39514)
                     /locus_tag="OO135_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5396033.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMIGGAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(39616..39747)
                     /gene="sgrT"
                     /locus_tag="OO135_000040"
     CDS             complement(39616..39747)
                     /gene="sgrT"
                     /locus_tag="OO135_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5396034.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            39836..41491
                     /gene="sgrR"
                     /locus_tag="OO135_000041"
     CDS             39836..41491
                     /gene="sgrR"
                     /locus_tag="OO135_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5396035.1"
                     /translation="MSSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      41525..41624
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41655..42638
                     /gene="thiB"
                     /locus_tag="OO135_000042"
     CDS             41655..42638
                     /gene="thiB"
                     /locus_tag="OO135_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5396036.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVQFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            42614..44224
                     /gene="thiP"
                     /locus_tag="OO135_000043"
     CDS             42614..44224
                     /gene="thiP"
                     /locus_tag="OO135_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5396037.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            44208..44906
                     /gene="thiQ"
                     /locus_tag="OO135_000044"
     CDS             44208..44906
                     /gene="thiQ"
                     /locus_tag="OO135_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5396038.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(45020..45784)
                     /locus_tag="OO135_000045"
     CDS             complement(45020..45784)
                     /locus_tag="OO135_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5396039.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(45870..46748)
                     /gene="araC"
                     /locus_tag="OO135_000046"
     CDS             complement(45870..46748)
                     /gene="araC"
                     /locus_tag="OO135_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5396040.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFVNTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            47086..48786
                     /gene="araB"
                     /locus_tag="OO135_000047"
     CDS             47086..48786
                     /gene="araB"
                     /locus_tag="OO135_000047"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5396041.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSLEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            48797..50299
                     /gene="araA"
                     /locus_tag="OO135_000048"
     CDS             48797..50299
                     /gene="araA"
                     /locus_tag="OO135_000048"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5396042.1"
                     /translation="MTIFDNYDVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            50499..51194
                     /gene="araD"
                     /locus_tag="OO135_000049"
     CDS             50499..51194
                     /gene="araD"
                     /locus_tag="OO135_000049"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5396043.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            51269..53620
                     /gene="polB"
                     /locus_tag="OO135_000050"
     CDS             51269..53620
                     /gene="polB"
                     /locus_tag="OO135_000050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5396044.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     NCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            53784..56690
                     /gene="rapA"
                     /locus_tag="OO135_000051"
     CDS             53784..56690
                     /gene="rapA"
                     /locus_tag="OO135_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5396045.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKMVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            56702..57361
                     /gene="rluA"
                     /locus_tag="OO135_000052"
     CDS             56702..57361
                     /gene="rluA"
                     /locus_tag="OO135_000052"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5396046.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAPDNTARVVLKPITGR
                     SHQLRVHMLAQGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(57478..58293)
                     /gene="djlA"
                     /locus_tag="OO135_000053"
     CDS             complement(57478..58293)
                     /gene="djlA"
                     /locus_tag="OO135_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5396047.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            58548..60902
                     /gene="lptD"
                     /locus_tag="OO135_000054"
     CDS             58548..60902
                     /gene="lptD"
                     /locus_tag="OO135_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5396048.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            60955..62241
                     /gene="surA"
                     /locus_tag="OO135_000055"
     CDS             60955..62241
                     /gene="surA"
                     /locus_tag="OO135_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5396049.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            62241..63230
                     /gene="pdxA"
                     /locus_tag="OO135_000056"
     CDS             62241..63230
                     /gene="pdxA"
                     /locus_tag="OO135_000056"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5396050.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            63227..64048
                     /gene="rsmA"
                     /locus_tag="OO135_000057"
     CDS             63227..64048
                     /gene="rsmA"
                     /locus_tag="OO135_000057"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5396051.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            64051..64428
                     /gene="apaG"
                     /locus_tag="OO135_000058"
     CDS             64051..64428
                     /gene="apaG"
                     /locus_tag="OO135_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5396052.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            64435..65277
                     /gene="apaH"
                     /locus_tag="OO135_000059"
     CDS             64435..65277
                     /gene="apaH"
                     /locus_tag="OO135_000059"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5396053.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(65355..65834)
                     /gene="folA"
                     /locus_tag="OO135_000060"
     CDS             complement(65355..65834)
                     /gene="folA"
                     /locus_tag="OO135_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5396054.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(66026..67888)
                     /gene="kefC"
                     /locus_tag="OO135_000061"
     CDS             complement(66026..67888)
                     /gene="kefC"
                     /locus_tag="OO135_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5396055.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAEVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMSDEPETKPSS"
     gene            complement(67881..68410)
                     /gene="kefF"
                     /locus_tag="OO135_000062"
                     /pseudo
     CDS             complement(67881..68410)
                     /gene="kefF"
                     /locus_tag="OO135_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
     gene            complement(68518..69765)
                     /locus_tag="OO135_000063"
                     /pseudo
     CDS             complement(68518..69765)
                     /locus_tag="OO135_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT        16862 a        19330 c        17704 g        15869 t
ORIGIN      
        1 ggattagata cctgcgacgg tggcagcaat ggcattccct cgcctaccac cacccgctat
       61 gtttcagcca tgagtgtggc aaaaggcgtg gtgtcgctga ccgggcaaga aagtctcaat
      121 ggactaagcg tcgtcatgac gccaggttgg gataacgcaa acggcgtcac cggctgggcg
      181 cgcaactgca atattcaaag tgacagcgca ttgcagcaag cctgcgaaga tgtcttccgc
      241 tttgatgacg ccaactaagg agcggcaatg aatattccac agctcaccgc cctgtgtctg
      301 cgttatcagg gagtcttgct ggatgccagc gaagaggtgg ttcatgttgc ggtagtcgat
      361 gcaccttcgc atgagttgct ggacgcattg catttcgcta ccaccaaacg tattgagatc
      421 acctgctgga cgcgccaaca aatggaaggc cacgccagtc gcacacaaca gacattgccc
      481 gtagctgttc aggagaagca tcagtcgaaa gcagagttgc tgactcgaac gttacaatct
      541 gcgctggaac aacgtgcgtc tgatattcat atcgaaccag cggacaatgc ctaccgcatc
      601 cgcttgcgta tcgacggcgt attgcatcct ttaccggatg tttcaccgga tgccggagtc
      661 gcattaaccg ccagattaaa agtgctggga aacctggata ttgcggaaca tcgcctgccg
      721 caggacgggc aattcactgt cgaactggca ggaaacgccg tctcatttcg tattgcgacc
      781 ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt tacagcaggt gaaccaggca
      841 ctggatgtta acacgctggg aatgcagccg ttacaactgg cggactttgc tcatgccttg
      901 caacaaccac agggactggt gctggtaact ggccctacag gcagcggcaa aacggtcacg
      961 ctttatagtg ccctgcaaaa gctgaatacc gctgacatta atatttgtag cgtcgaagat
     1021 ccggttgaga tccccatagc cggactaaac cagacgcaaa tccatccgcg tgccgggctc
     1081 acctttcagg gcgttttgcg tgcgttattg cgccaggatc ctgacgtcat catgatccga
     1141 tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc ggtcacctgg tgttgtctac
     1201 cctacacact aattccacct gcgaaacgct ggtacgttta cagcaaatgg gagtcgcccg
     1261 ctggatgctc tcatcagcgc ttacgctggt aatagcccag cgtctggtac gtaaattttg
     1321 cccacattgt cgccagcagc aaggggagcc catccatatt ccagacaatg tatggccgtc
     1381 gccgctgccc cactggcagg cacccggttg tgtacattgc taccacggtt tttatggtcg
     1441 taccgccttg tttgaagtcc tgcccattac accagccata cgccagctta tttccgctaa
     1501 taccgacgtt gaatcgctgg aaacgcacgc acgacaggcg ggtatgcgta cgctttttga
     1561 aaacggctgc ctagccgtgg agcaaggctt aaccaccttt gaagagttaa tccgcgtact
     1621 ggggatgccg catggcgagt aagcaactct ggcgctggca tggcataacc ggcgacggca
     1681 atgcgcaaga tgggatgcta tgggcagaga gccgtgcttt gctgctcatg gcactacagc
     1741 aacagatggt taccccactt agcctgaagc gaatcgccat caattctgcg cagtggcgag
     1801 gagataaaag cgcggaagtc attcatcaac tggcgacgct actcaaagcc gggttaacgc
     1861 tttctgaagg gctggcactg ctggcggaac agcatcccag taagcaatgg caagcgttgc
     1921 tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt ttccaatgcc ttattaccct
     1981 ggtcagaggt atttccgcca ctctatcggg cgatgatccg cacgggtgaa ctgaccggta
     2041 agctggatga atgctgcttt gaactggcgc gtcagcaaaa agcccagcgt cagttgaccg
     2101 acaaagtgaa atcagcgtta cgttatccca tcatcatttt agcgatggca atcatggtgg
     2161 ttgtggcaat gctgcatttt gttctgccgg agtttgccgc tatctataag accttcaaca
     2221 ccccactacc ggcactaacg caggggatca tgacgctggc agactttagt ggcgaatgga
     2281 gctggctgct ggtgttgttc ggctttctgc tggcgatagc caataagttg ctgatgcgcc
     2341 gaccgacctg gcttatagtg cggcagaaat tgctgttacg catcccgatt atgggttcac
     2401 tgatgcgggg atctttacga ttctggcgct gacacaaagt gcaggcatta cttttttgca
     2461 gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac ttctgacaca
     2521 aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa ataccgggga
     2581 gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct cgctggacct
     2641 catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg cggataacct
     2701 cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg gtacgctggt
     2761 ggtggcgatg tatctgccaa ttttccattt aggcgatgcg atgagtggga tgggataacg
     2821 ctggcgtgtt gctccacacc agcgttggaa gattacaggt tgttgaagat gcggttttct
     2881 tgttcctgca cacggataaa tgtggtgcgc ttggtcagtt ctttcaggcg tgaagcccca
     2941 acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt ttcaaccggg
     3001 cctcgcagcg gcagcttaac ggttttacct tctgctgcgc gatattccgc aacgccgcca
     3061 acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa tttctcgccg
     3121 ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc gccaagcatg
     3181 acgaaatcgg caccgccgcc aaaggctttt gcgacatcgc ccggcgtggt gcagccaccg
     3241 tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat taccgcagaa
     3301 agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc tgggccaatg
     3361 ccaactttaa cgatatcagt accggagagg ataagctcct cacacatttc accagtcact
     3421 acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc aacgaactgc
     3481 acgaagtgtt cggaataacc attcgccaca tcaatacaaa caaagtttaa tgccgggttc
     3541 aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtaccggt agaaaccatc
     3601 acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc ttcgacagaa
     3661 tagtgtttat gcacagcagt caaaatatca aaagaagcca gcgcagaggc catagaaaat
     3721 gtacctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct ctgatctgaa
     3781 tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag agtggagcgt
     3841 ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat acgcatgtgc
     3901 ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg ctatcatacg
     3961 cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca atcccataaa
     4021 tttacctggt aaatagtgag cgaacgtttc atcaatttcg ctttcgtgca ctcattttta
     4081 acgttttgat aatcatgatg aattcccggg aatataaatt atgaggtata tagttgcctt
     4141 aacgggaggc attggcagtg gcaagagtac cgttgccaat gcgtttgctg atctcggaat
     4201 taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg cacctgcgct
     4261 acatgccatt gctgatcact ttggcgctaa catgattgct gctgatggaa cattgcagcg
     4321 ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc ttaacgccct
     4381 gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcta cttcccccta
     4441 tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag cgaatcgagt
     4501 gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc gcgatgatgt
     4561 aactcgcgag catgtcgaac aaatccttgc tgctcaggca acgcgcgaag cccgccttgc
     4621 cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat cggatgttgc
     4681 ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg aaaaaccgta
     4741 atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac atggctgcgc
     4801 attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca cgctggcgcg
     4861 ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg cgaagtccgc
     4921 actgagctgt tgaaagaact tgaccggcag caacgtaaac tccagacctg gattggcgtg
     4981 cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc ggcggggagc
     5041 gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt gattgctctg
     5101 gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc tacattgcac
     5161 atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg gattgccagc
     5221 ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca gtcggccccc
     5281 ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga tgccgacttg
     5341 ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg acataagagc
     5401 cgttttgcca ttcgttttat gccgctggac agtgaaaacg gacaggtacc ggaacgtcta
     5461 gatttcgaac tggcctgttg ctaaggagtg aaaatgtcag aaactattac ggtgaattgc
     5521 ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc attttgctcc
     5581 aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg aatccccagc
     5641 agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg acattggtct
     5701 gatgccgcac ataggcctga taagacgcgg acagcgtcgc atcaggcatc ttgtgcaaat
     5761 gtcggatgcg gcgtgaacgc cttatccgac ctacagacta cagacgctta agcttcgcaa
     5821 ttaccggttc attggctggc ggaaaatcat cggcattaag accgaccagc gacatccact
     5881 caccgggttg cccttcttta ccccacggca ccccttccca actttcgacc agccagaacc
     5941 acagagttat atgcctgtcc gggaattcat atgccagttt ttcaaatagc gaaaaatgtt
     6001 gtggggtaat cccgacctct tcctgaagtt cacgcaccac cgcctgttcc ggcgtttcgc
     6061 ccatttcaat tttaccgccg ggaaactcca gtttattcgc catgtgcgca tctgctgcgc
     6121 gacgcgttat aaagatttca ttgttctcgt tgcgaataat acctaccgca atttgcagct
     6181 ttttcattgt tttaaaccta taaaaaaggc gcagaatcct gcgccttttg cttcaacagt
     6241 tagcttttat tgcaggcggc catgacactg cttgtatttt ttaccagaac cgcacgggca
     6301 aggatcgtta cgtcctactt tgcgctcacc ggtttgcgcc gccagtgcag ctgcggctgc
     6361 agagtcgtca tcctgatggc taagctgctg catttgcgct aaacgctcgg cttccatacg
     6421 acgctgttgt tccagctcct caacctcttc aggcatacgt acctgaactt tgctcagcgt
     6481 actgataact tcatatttca acgactccag catcgctgca aacatggaga acgattcacg
     6541 tttgtattcc tgcttcggat ctttctgtgc atagccacgc aggtggatac cctgacgcag
     6601 atagtccatc gctgccaggt gctctttcca cagggagtca agcgtttgca gcatgacgcc
     6661 tttctcgaag tgacgcatca tctcagcacc aaccacttct tctttacgct gatacacttc
     6721 gatggactgc gccagaatgc gctcacgcag cgtctcttca tgcagttctg gttctttatc
     6781 cagccactcg gcaattggta aatcgaggtc gaaatcgttc ttcagacgtt cctgcagccc
     6841 cggaatatcc cacatttctt ccagcgactg tggcggaatg taggcatcaa tggtggcttt
     6901 gaacacatct tcacgaatgc tgttgatggt ttcgctcaca tcgctgacat ccagcagttc
     6961 gttacgctgg gagtaaatgg cgcgacgctg atcgttagcc acgtcatcat attccagcag
     7021 ttgcttacga atgtcgaagt tacgactttc aactttacgc tgggcgttgg caatcgcttt
     7081 agtcacccac gggtgttcaa tggcttcgcc tggcttcata cccagtttac gcatcatgcc
     7141 ggatactcgg tcggaagcaa aaatacgcat cagcgcatct tccatcgaca ggtagaaacg
     7201 ggaagaacca gcatccccct gacgaccaga acgaccgcgc aactggttat cgatacgacg
     7261 ggattcgtga cgctcggtac cgatgatatg caggccacct gcttccagta ccgcatcgtg
     7321 acgtacctgc cagtcggctt taattttttc aatttgctct gcggtcggat tttccagcgc
     7381 ggcaacttct gcctgccagc taccaccgag cacaatatct gtaccacgac ccgccatatt
     7441 ggtcgcgata gtcaccgcag ccggataacc tgcctgagca acaatcgccg cttcgttggc
     7501 gtggaatttg gcgttcagga cgttgtgctt aataccggct ttggtcagtt cgtttgacac
     7561 cagctccgat ttttcgatgg agatagtacc caccagcacc ggctggcctt tcgcagtacg
     7621 ttctttgata tcttcaatga tcgcctgaat tttttccgct tcagtcatgt agaccaggtc
     7681 cggcagatct ttacgaatca ttggacggtt ggtcggaaca acgacggtat ccagcttgta
     7741 gatggagcta aattcgaaag cttcggtatc agcagtaccg gtcatccccg ccagtttttc
     7801 atacagacgg aagtagttct ggaaggtgat cgaagccagc gtttggtttt cgttctggat
     7861 ctgcacacct tctttcgctt ccacagcctg gtgcagacca tcggaccagc gacggccctg
     7921 catggtacga ccggtgtgtt cgtcaacgat gataacttca ccatctttaa cgatgtagtc
     7981 gacgtcgcgg gtaaacagcg catgagcgcg cagcgccgcc gttacgtggt gcatcagcat
     8041 gatgttggcc ggagagtaca gagactcccc ttcatccatg atgccctctt tcaccagcag
     8101 ttcttcaatc agcaccagac cacgttcggt caggttcacc tggcgagatt tttcgtccac
     8161 cgagaagtgg ccttcgccct ggaaggtttc ggagtcttct ttttcctgac ggatcaggtg
     8221 cggaataatt ttattcacgc gtttatacat ttccgagctg tcttctgccg ggccggaaat
     8281 gatcagcggt gtacgcgctt catcgatcag gatggagtcc acttcgtcca ccagcgcata
     8341 gtgcagttta cgctgtacac gttcttcagg gctgaacgcc atattgtcgc gcaggtagtc
     8401 aaagccgtat tcgttgttcg taccgtaagt gatgtcagct gcgtaagctt cgcgctttgc
     8461 cggtgctggc atgcccggca ggttgatacc gacagtcagg ccaaggaatt caaacagcgg
     8521 acggttgttt tcggcgtcac gttgcgccag gtagtcgtta acggtaacta cgtgcacgcc
     8581 tttaccggtt agtgcgttca ggtaagcagg cagcgttgcg gtcagggttt taccttcacc
     8641 ggtacgcatt tcggcgatgc agcgttcgtt aagaaccata ccgccgagta actgaacgtc
     8701 gaagtgacgc ataccaaaga cgcgcttact tgcctcacgt accacggcga aagcttccgg
     8761 gatcagattt tccagcactt cgcctttttc cagacgtgca cgaaactctg cggttttccc
     8821 tttcagttct tcgtcggaga gtttttccat ctccggttcc atggcattga tgatgttgac
     8881 cactttgcgc atccggcgca gggtgcgatc gttacgacta ccgaaaactt tagttaacaa
     8941 tttgattagc ataataaaat ctcaaacgcc ccgcgttgcg gagttaataa aatgaagtaa
     9001 aggtttattg ttgttaggtg aggcgttgag ggccagcacg gatgccttgc gcctggctta
     9061 tccagacggg cgtgctgaat tttgcttgtg gggtaaaatg cgcataatca atgcgataac
     9121 ccttttcaga cggcgtgcct tcctgggtca gcagcgcgct gagcgtatcc agtaatgcaa
     9181 gatgttgcgc ctgaagaggc aaagattctt cagcaacggg cagtgtttgc ggtgccattg
     9241 cgaaagaaag atgacggatt accgtgcgaa tggcatattg atgccagtaa tcaacggaat
     9301 agttcgaatt cgggcggcgt gtgttcgctt ccagcaaggc caattgacca aagttaactt
     9361 tggctgaagg ctcgtggttg cgggttgtcg cttttgcggg cacgtttggt tcggcggcgt
     9421 tgctgagcgc aggcaaacct aaactcgccg cgaccatccc taataagaga tgcggccaga
     9481 agtagcgttt accaaactgt cgccagcgcg tcagtattcc actcacgtta ttgccatccc
     9541 gatcgaattc aaacgtattt agcgcacaaa tcttatcatt aaagcctgaa gcccacagca
     9601 tgaatgaaga taagttgtgc gtaaaagtgc ttaagaaaac agcgttcgca ccgtttttta
     9661 tagctcttat aaaattcaaa aaaaacgact ggttcacctg gccggagaga gtgccagatt
     9721 tgccagtcga attttatacg acagtataaa tgtcgttatg ccagtacagc tgaaggcgct
     9781 ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca catattccca ggcttcctgt
     9841 ttcgccagga cagcctgcag cagtttgtta ttcagtgcat gaccggattt ataagcgata
     9901 aatgcaccaa taatattgtg accacacatg aacaagtcac cgatcgcatc gagcattttg
     9961 tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt tcagtacgcg ataatcgtca
    10021 acaacgatgg cacaatcgaa gctgccgccc aggcacaaac cacgggactg cagatattcg
    10081 atatcacgca tgaaaccgaa cgtacgcgca cggctgatct ggcgcataaa cgcatcagcg
    10141 gagaagttca tcgcatagcg ctggttgctg gaatcaatag ccggatggtt aaaatcgatg
    10201 gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag cccacttatc gccatcttcg
    10261 acacgaacag tctctttgat gcgaacaaat tttttggcgc agttcaactc gtcgataccg
    10321 gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt ccatgatcgg gatttccggc
    10381 gcgttaactt cgataacaat gttatcgatg cccaggcccg cgagagcagc attgaggtgc
    10441 tctacggttg aaatccgtac atcatgctcg ttgaccagac acgtacagag catggtatca
    10501 cgcacagatt tggcatcggc cgggaaatct accggtggat tcaagtcggt gcgacgatag
    10561 atgaccccgg tgttggccgg cgcagggcgt aacgtcagag tgactttctt gccggtatgt
    10621 aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc tttgtttgat catcgtatta
    10681 tctcgccaaa ttacctatct aaccgaagtg tactatacat tcggcgggcc agtttagcac
    10741 aaagagcctc gaaaccaaaa ttccagtcaa ttcttaatca gcttgcttac gcaggaatgc
    10801 tgggatatcc agataatccg gctctttcgc agtttgcggc gcattgtcat tcacgacttt
    10861 agcaaccggc ttctgctcct gggtcagcgg agccatccca tgctgctggt agcgatccat
    10921 cactggctgc tgaacctgct tattggtcac cagagtgatt tcaggacgtt tgtccatgcc
    10981 gatacctgtc gcaacaacgg ttacgcgcag ctcgtcattc atatccgggt caagagaagt
    11041 accgataacc acagtcgcgt tgtcggaagc aaatgcacgg atggtgttac ctaccgtttc
    11101 gaactcatcc agacgcaggt cgaagcccgc cgtgatgtta accagcacgc cgcgcgcgcc
    11161 agacaggtcg atatcttcca gcagcggaga agagatagcc atttcagcag cttcttccgc
    11221 acggtcttca ccgctcgcca cgccagaacc catcattgcg tagcccatct cagacattac
    11281 ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga cgagtaatca gttcagcgat
    11341 accttgcaca gcgcctttca gtacatcgtt cgctgcgcca aacgcatcca gcagggagat
    11401 accgcggccc agaactttca gcagtttgtc gttcgggata gtgatcagag agtccacatg
    11461 cttggacagt tcagtgatcc cctgctccgc gaatgccata cgcttcttgc cttcaaagtt
    11521 gaaaggctta gtgacgacag caacggtcag gatacccaaa tcttttgcca cttcagcgac
    11581 gactggtgct gcacctgtac cggtaccacc acccataccc gcagcaataa agaccatgtc
    11641 tgcaccttcc agcgccgcac gcaatgcatc gcgatcctca tcagccgcat tgcggccaac
    11701 ttctggatta gcgccagcgc ccagtccttt ggtgataccg ctaccgattt gaatcgtctg
    11761 tccaaccgct gttttacgca gcgcttgtgc atcggtattt accgcgaaga attcaacacc
    11821 ttcaatgcgc tcgcgcacca tgtgttcaac agcattaccg ccgccgccgc cgacgccgat
    11881 gactttaatc accgcgtcat tggtaagttc cattggttca aacatagttt ctctccgatt
    11941 tgtgcctgtc gcctgaggcc gtaatcatcg tcggcctcat aaaaattaaa actcttttcg
    12001 cagccaacta ttgagtcgct tgatccacga gccaactgat gctgtaacac gtttttctac
    12061 ttcagcttca ccgttaagat gtgactcttt cccatagtga agcaatccca ccgccgtcga
    12121 ataatacggc tcctgagcat aatccgttaa accggtaatg ttcagcggcg cgccgatacg
    12181 cacttgcgta tgaaacacgc gctgagcaca ggctgcaaga ccttcgatct gcgctgcgcc
    12241 accggttaat acaatgcctg ccgccaggtg atgtttaacc ccttgttggc gaagcttttc
    12301 ctgcaactgc aatatctctt cgttgaccag gttgagcagc tcggtatagc gcggctcgat
    12361 cacctctgcc agggtctgac gttgcagact ccgtggcgga cgaccgccta cgctcggcac
    12421 ttccacgctc tcatcttttc caacgatgga acccagcgca caaccgtggc gaactttaat
    12481 cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg atatcactgg tcacgacatt
    12541 gccagcataa ggaattacct tagtgtggcg caatgcccca ccggtataaa cggcgatatc
    12601 cattgtacca cctccgatat cgacgacgca gacacccagt tcacgttcat cttccgtcaa
    12661 taccgaataa cttgatgcca gtccggcaaa tatcagttgg tcaactttca gcccacaacg
    12721 ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga catgtgatca ggtgcacttt
    12781 tgcctgcatc cgcacgcccg aaagtcctac cggattcttg atcccttcct gatagtcaat
    12841 cgcatactct tgcgggatca catgcagcac acgatgttca tcgcgcacac gcaccgattt
    12901 cgcggtatgg acgacgtttt ccacatcttc ttgcgtcact tcttcttcag aaataggcac
    12961 cataccaatt tcattctggc agctgatgtg cttaccagaa agcgccagat ataccgaaga
    13021 gatctgacaa tctgccatca attctgcctg gtcaatggcg cgttgtacgc acttgaccac
    13081 ggattcgagg tcgttcaccc cgcctttatc cataccacgt gacgggcagc tgcccacgcc
    13141 aatgatattg accataccgt cgggcagaac ttcccctact aaagcggcaa ccttcgcggt
    13201 accaatctcc agtcctacta ccagttttct gtccgtcgcc ttgatcattg ttgttctgcc
    13261 tgtgcctgat tttgttgctg agtagattcc tctggcggca agggcgccca gcctactgcc
    13321 gctccagagt cataacgcaa atcaacgtag ctaatccgtt tgccatcggt ttgcgcctgc
    13381 tgctgtaaaa ccggataaag ttctacaaag cgagccaaac gtttcatcgt atcgccccgg
    13441 ccaagattga gcttaatatc gttattcagc gtcaactgcc aggaacgccg cgcggtcatc
    13501 gccgcttcct tcagagtaaa tctgtccttt gccagcatct gccccatttc gcgatagccc
    13561 tgcaacactt cattggcgct gccttccggg ccatacagca ttggaagcac ctgcttgctg
    13621 gtgcgatctg gcggcacgct gaaggtgttt ccttccgcgt ctaccatatg ttgatcattc
    13681 caccgcgcaa tcggcacata ttcaaccaga tgaatcttca attcatcagg ccactgcttt
    13741 ctgacgctca cctgcttaat ccacggcagg cgttgttcta tttgcgtctg gatgatgttg
    13801 acatcctggg tcataaaggt acccggctca cccaatgcca ggatcgactg ccggatatcg
    13861 tcattacgcg tgtaatggcg ttcaccggtc aacaccagct ttgagagcgg caggcgttgc
    13921 gcatcttcca tccagcccaa cacgacccag ccgctcacca acactgtcgt taaaacggtc
    13981 agcaggaaaa ggatccccgc cagacgcgtt ccattattgc ggcgagaaga aacctcttct
    14041 tcgctgtttc gcgtgttcag agcagcctgc gacatattag tccgccagtt ccagaattcg
    14101 tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc gccatcggca ccaggctgtg
    14161 gctggtcata cccggtgagg tattggcttc cagcagataa aactgtccat cgctgtccag
    14221 cataacgtca atacgtcccc atcctttgca acctaacgtc gtccatgctt tcagcactaa
    14281 tgcctgcaaa ttggcctctt gtgacgcatc cagacctgcg gggcagaaat actgtgtctc
    14341 atcagagaga tacttcgcct cataatcata gaaggttccg gacggttgaa tacgtattga
    14401 cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc ggcccactta gccatttttc
    14461 aatcaatact tcttcatcgt gctgaaatgc caatcttaat gcatcttgta gagcattttc
    14521 tgctactact tttgacattc ccacactgga accttcgcgg ctcggcttaa cgataaccgg
    14581 caaacccaga gcagaaattt ctgctaactg cttatcgctc aggccttttt caaactctgc
    14641 gcgggttaac gctacccacg gcgcgaccgg taaaccggca ccttgccaca gcagtttgct
    14701 gcgtagttta tccattgaaa gcgcagatgc catcactccg ctgccagtat aaggcaagcc
    14761 catcagctcg agcatgccct gcagcgtacc atcttcaccg ccgcgaccgt gtagcgcgat
    14821 aaacactttc tgaaaaccca tcgacttcag ttgcgtcacg tcgacttctt tcgggtcgac
    14881 aggatacgca tcaataccac cttcacgcag cccggctaac accgctgcgc cagaattcag
    14941 agaaacttcc cgctcagcgg aagtcccgcc caacaggacc gcgattttat cagtcatgtt
    15001 gttcttcctc cggagtttgc ggcttcagtt tgatttcagc taaagaacgg gcaatttttc
    15061 cgatattacc agccccctga acgagaatca ggtcgttacc ggttaatacc ggtgccagca
    15121 tctcggctac ctgcgcaggg tccggcacca gaatgggatc aattttccca cgtccacgaa
    15181 ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg cgcttcgcca gccggataca
    15241 cttccagcat caacagggta tcaacctgcg tcagcacatt ggcgaaatca tcatacaggt
    15301 cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac caggttttta tccggccagc
    15361 ctgcgcgcgc cgctttaatg gtggcgtcaa cttccgtcgg gtggtggccg tagtcatcga
    15421 ccagcattgc cgtaccgctt ttaccattca ctggctccag cgggaattca ccgaggaaat
    15481 caaaacggcg accagtcccc aggaagctct ccagcgcacg caaaatagcc tcgtcgtcaa
    15541 ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt cagcgcgtta tgacggcctg
    15601 gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg cagcagcgta aagtgcccct
    15661 gcgggccaat ctgctgataa tcttctacac gcacgtcggc atcttcgctg aagccgtaag
    15721 tcgtggtctg acgtcccaca cgcggtaaca attcgcggat caccggatca tcaacacaca
    15781 tcaccgcacg accgtaaaac ggcaggttgt gcagaaaatt aataaaagtc tgttttaaat
    15841 tctcaaagtc gccctggtag gtatccatgt ggtcggcttc gatattggtg acaatcgcca
    15901 ccatcggttg cagatgcagg aacgatgcat cactctcatc tgcttcggca atcaggtacc
    15961 gaccatgccc caaacgcgca tgaacccccg ccgcttttac cagcccgccg ttaacgaagg
    16021 ttgggtcgag ccccgcttct gcgtagatgc tggaaaccat cgcggtggtt gtcgttttgc
    16081 cgtgcgttcc tgcaatggcg atgccatgac gaaaacgcat taactcagcc agcatttcgg
    16141 cacgacggat caccggaata cgcgcttcat gagctgcgac aatttccggg ttatcggcag
    16201 aaatggcgct ggaaacaacg accacgctgg catcacgtac gttttccggg cgatggttga
    16261 aataaatcgt cgctcccaga ttcattaact gctgcgtgac cggatttggc gctaaatcgg
    16321 aaccactgat ctgataacct tcattggcca gaacttcggc aataccgccc ataccggcac
    16381 caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat ttcgggcacg atggtacgca
    16441 gttttgccaa ttgttgtgta ttcattcttt acgccattaa cttcttaaat tcatacgatg
    16501 caaaaggcat cgctacaatt acgcccgggc agcccggctc acttcatttg ccactcgctc
    16561 ggtggcatcc ggaatggatg cagcgcgggc gcgttctgcc atggttaata aggtttctcg
    16621 cgaccacccg gccagggtgt tggcgacagc atccacgcta agctgtggct gctcgataat
    16681 tttggctgcg cccgcttttt ccagcggtag cgcattccag tattgctggc ggtctttatg
    16741 ttgaaacggc acaaacaacg ccggtagtcc tgccgcggcg atttcactca ccgttaaagc
    16801 accggagcgg caaacgacga catccgccca cgcatacgcc gccgccatat catcaataaa
    16861 ttccgtcact ttatgctgcg gttgccccgc ttcggcatac gcctgttcaa cggattgttg
    16921 cgaaccttta ccgctctgat gccagatagt gactgaatca cccagtttcg cagcaacctg
    16981 cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca cccactacca gcacacgaac
    17041 cggaccttca cgtccagcca aacgttgctg cggcaacggc agcgccagca catcggtacg
    17101 caccgggtta cccaccactt ccgcattagg gaaagcgcct ggaaacgcct gcatcacttt
    17161 ggtggcaatc ttcgccagcc atttattggt taagcccgca ataccgtttt gttcatgaag
    17221 tacaaccgga atgcctaacg accacgcggc cagaccacct ggacctgata catagccgcc
    17281 cataccgagt accacgtcag gtttgtacgc tttcataatc gcccgcgcct gacgccaggc
    17341 gttgaagata cgcagcggcg cagctatcag tgcttttata ccttttccac gcagaccaga
    17401 gatacgaatg aaatcaattt cgatgccatg ttttggcact aagtccgctt ccatacggtc
    17461 ggcagtcccc agccagcgaa cttgccaacc ctgagccatt agatggtgcg caaccgccag
    17521 tcccgggaat acatgtccac cggttccgcc tgccatcacc attaatcgct ttccttgacc
    17581 actcatcgtg aacctcgtac aaacgcctgc gctttctcca gacgcgtttc ataatcaata
    17641 cgcaacagca tcatgatggc tgtcgacata atcagtaagc tcgaaccacc gtaactgatc
    17701 agcggcaatg tcagaccttt ggtcggtaac atccccgccg ccgcgcctac gttaaccagc
    17761 gcctggaagc taaaccagat gccaatagaa caggcgagaa aaccggaaaa acggtggtca
    17821 atttctaacg ctttgcggcc aatcgacatc gcgcgaaaag cgacgaagaa taccattaaa
    17881 agtgccagca ccacaccgac ataccccagt tcttcgccga taatggcgaa aataaagtca
    17941 gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt tacctaaacc ttgtccccaa
    18001 agttcgccgc gaccaaacgc catcagcgat tgcgttaact gatagccgct gccaaaggga
    18061 tcttcccacg ggttccagaa tgcggtaaca cggcggatac ggtacggttc ggcgagtatc
    18121 agcaacacaa ccgctgaaat gcccataccg ataatggcaa tgaactgcca caatttcgct
    18181 cccgccagga acaacatcgc cagcgtagtc acaaacacca ccaccaccgt accaaggtct
    18241 ggctgtgcca gcagtaacac tgccaacacc agaatcacgc ccatcggttt caggaagccg
    18301 cgcaggttat tacgtacttc gtcgccttta cgcaccagat agttggcgat ataacaaaac
    18361 agcgacagtt ttgtcagctc cgcaggctgg atacgcagca aaccgagatc gatccaacgc
    18421 gatgcccctt taaccgagct acccactacc agcacgatca tcagcaggat gatagatccg
    18481 agcagcatcg tggcactgta gcgttgccag aactccatcg gcagacgcag cgtaatgatc
    18541 gccagaatga atgccaggat cagatagacg ccatcacgct tcgcgaagaa gaacggatcg
    18601 ttggttaagc gttgccctat gggcattgac gccgaggtca ccatgataaa gccaatcgcc
    18661 gcaaggccga aggtcagcca cagtaaggtg cgatcgtaca tgatcaggct gtcggtatct
    18721 ttttcccgcg agcccatcac ccagcctttt agcgccgtgg agatccagac caggatactg
    18781 aatcctggca ggcgcggcat tttcaggcga gggagagata aacgcatcaa cctaactcct
    18841 tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt cttgaactga tcaaggctgg
    18901 cacaggccgg ggagagcaga accatatcgc ccggctgaac acgcggagcc agcaagcgca
    18961 tcgcctgttc catagtttcg gtttgttctg ccacttccgg gcgtagcgcc gccagctgcg
    19021 caccgtcacg accaaaacag tacaaacgca cgttgtcgcc attcagatag cgcgccagtg
    19081 gactgaagtc cgccgattta ccatcgccgc ccagcaacaa atgcagcgtg ccgtctacct
    19141 gcaagccatt caacgccgct tccgtactgc cgacgttagt ggctttcgaa tcgttaatcc
    19201 agcgtacgcc attgtgctcc agcaccactt caaagcgatg cggcagaccg gtgaaggtgg
    19261 ttaacgcttt caggctgctg gcgcgcggca aaccagctgc gtctgccagt gccagtgctg
    19321 ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat ctctttcacg ttcagcactt
    19381 tctcgccttt cactcgcagc caggtttctc cctgctgatg attcaggtga tagtcaccca
    19441 tgttgacgcc aaagctgacg cagcgttcat cagcaccgcg aatcggcatt gttaaggcat
    19501 catcagcatt gacgacacaa actttcgcat tttcgtaaat acgcaatttt gccgcacggt
    19561 attgctgcaa accaaacgga tagcggtcca tatgatcttc agtcacgttc agaatggtcg
    19621 ccgccaccgc ctgtaagctg gaagtggttt ccagctggaa gctcgacagt tccagcacgt
    19681 acagttcgca ctcagcatca agcagcatca acgctggcag gccaatattg ccacccacac
    19741 caacgttaac ccccgccgct ttcgccattt cacccactag cgtggtgacc gtgcttttgc
    19801 cgttagaacc ggtaatcgcc acaatcggtg cttgtgcttc gcggcagaac agctcgatat
    19861 cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa ggatggatgc gccagtgcaa
    19921 taccgggact ggcgacaatc agatctgccg ccatcagcca ttcatcattc agactgcccg
    19981 tgtggcgttc tacggcttcg ggtaatttat ccaggctagg cggtgtcata cgcgtatcca
    20041 taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac gcaggaaagc ccggtgaggc
    20101 ccaggccgat aatgacgaca tttttaccct gataatcagc catgattaac gtaccttcag
    20161 cgttgccaga ccaatcagaa ccagcatcag cgaaataatc cagaaacgca caatgacgcg
    20221 cggttccggc cagcctttca gttcatagtg gtgatgaatc ggtgccatgc ggaaaatacg
    20281 ttgtccgcgc agtttaaagg agccgacctg caggatgaca gaaagcgttt ctaccacgaa
    20341 cacgcccccc ataatcacca gcaggaattc ctgacgtagc agtacggcga taatgcctaa
    20401 cgcaccacct aacgccagcg aacctacatc gcccataaag acctgcgccg gataggtgtt
    20461 aaaccacagg aagcccagtc ctgccccgac tatcgcggta cagacaataa ccagttcccc
    20521 ggcgtgtcgc agatacggta tgtgtaaata gctggcaaag ttcatgttgc cggtcgccca
    20581 cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc ataattgcca ggccgtcgag
    20641 accatcggtc aggtttaccg cgttgccagt acccacaatg acgaagtaag ccagcagaat
    20701 gtagaacagc cccaactgcg gcatcacatc tttaaagaat gggaccacca gctgcgttgc
    20761 gggcgtgtct ttgccggcaa ggtacagggc gaaggcgaca cccagcgcaa tgaccgacat
    20821 ccagaaatac ttccaacgag cgatcaaccc tttggtgtct ttacgcacca ctttgcgata
    20881 atcatcaaca aagccaataa caccgtaacc taccagcacc accaacacgc accagacgta
    20941 cggattggac gggtaagccc acagcagtac ggagatcaca atcgccgtca ggatcataat
    21001 cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat tcaggaccgt cgttacgcac
    21061 cacctgacca aaggaaagtt tttgcaaatg agcaatcata cgcgggccca tccacaatga
    21121 gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac gtcagatagg aaaagacgtt
    21181 aaagccggaa taatatttga ccaaatgttc ggccagccaa actaacatgt cccattctcc
    21241 tgtaaagcgc gtactacctc ttccatggcg gcactacgtg aacccttaac taaaatcgta
    21301 attacctgtt gctcagcaat cagtgattta agacgcgtaa ttaacgcagt tttatcagca
    21361 aaatgttcgc caacgccgct ggcggtgctg atagcatggc tttgtttacc cacgcttaac
    21421 acgcggtcaa taccagcagc ttttgccgcc tcgcccacct gtacatggca ggcttcgctt
    21481 tcagcgccca gttccgccat atcgcccacc accagcacgc ggtagcccgg catttcagcc
    21541 agtacctgaa cagcggcggt cattgaaccg acattggcgt tgtaggagtc gtcgagcagc
    21601 aactggtttt ctgccagttg gatggggaac agacggcctg gaacagcttt cagatttgcc
    21661 agccccgctt tgatagcatc aagcgttgcg cccacggaca tggagagcgc agcggctgcc
    21721 agcgcattcg caatattgtg acgccccggc aacggcagca gaacatcgac gctaccggtt
    21781 ggggtttgta gggtaaattc cgtaccgtgc gaggtcacat ggatattggt ggcggtgaaa
    21841 tcgctgttgg cggcattggg tgagaaacgc cacactttgc gtgagccaat tacgctctgc
    21901 cagttcagcc agtcgttgtt gtcggcgttc ataatggcga taccgttttc cggcaggccg
    21961 ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg agccaaaacc ttccagatgc
    22021 gccgctgcca ggttgttgac cagcgcagct tccgggcgag tcagactcac agtccaggct
    22081 atttcgccct gatggttcgc gccaagttca ataactgcgt aatcgtattc cggcgttaag
    22141 cgcaacaacg tcatcggtac accgatgtcg ttgttgagat tgcctgccgt ataaagcgtg
    22201 ttgccgcact ggcttaaaat cgccgccgtc atctctttaa cggaggtttt gccggaggaa
    22261 cctgtcagag caaccacgcg cgccggaact tgctggcgaa cccatgcagc cagttcacca
    22321 aacgccagac gcgtatcctt gacgattaac tgcggcaggt cgatatccag cggacggctg
    22381 accagtagtg cgcccgcgcc gccagctttc gcctggtcgg caaaatcgtg ggcatcaaaa
    22441 cgttcgcctt tcagggcaac aaacaggcag cccggcgtca gttttcgggt atcagtggtt
    22501 acagcatcaa gggtgatatc tgcaccttgc agttcaccgt tgagaatgtc ggtaagttgg
    22561 ctaagggtta cgctaatcat gcaatcaccc ccagcagacg cgccaccgtg acgcgatcgg
    22621 agtagtccag acgctgattg ccaacaatct ggtaatcttc atggcctttg cccgcgacca
    22681 gcaccacatc gttctcttta gcctgcataa cggcgcaagt caccgcttca gcacggcctt
    22741 ccatcacttt ggcatgtccg gcatctaaca ttcccgccag aatatcgttg atgatggcac
    22801 gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc cacgtcagca aactcttcgg
    22861 caattgcgcc catcagtgga cgcttacctt tatcgcgatc gccaccacag ccaaagacac
    22921 accacagctt gcccgcacag tgcagacgcg ccgcctgtaa ggctttttcc agtgcatccg
    22981 gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg cgcagtgaac acttccatac
    23041 gtccgcaaac cggttgcaga cgcgcggcgg ttttcaacag atcagccaat ggatagccga
    23101 gtgccaacag tgtcgccagc gcgagcagca ggttgctgac gttaaaagcg cctatcagat
    23161 ggctttcaat ttcgccatcg ccccaacttg agctaaagcg aatcgtcaca ccgctgtcgt
    23221 gatagttcac gtcgatcact tttaaccagc gtccgtgaca gttcagatta atatgatctt
    23281 ccattgatac cgcaaccgcg tccggcagct ttgccagcca acggcggccc acttcatcgt
    23341 cggcgttaat aatcgcctga ccgcaatgat gttcagaata aagcagccat ttcgcagctt
    23401 cgtagtgttc catatcacca tgataatcaa ggtgatcgcg gcttaagttg gtaaagaccg
    23461 acgccgcaaa tttcaatgcc gccacacggt actgtaccag cccgtgggag gaaacttcca
    23521 ttgcgcaaaa cgtcgcgccc tgctccacca gccccgccag ctcatgctga acatcgactg
    23581 ccgaaccggt ggtattttct gtcgggatca ctttccccag cagaccgtta ccaacggtgc
    23641 ccattaccgc gctggtttcg ccaagcagtt ggctccactg cgccagcagc tgggtagtcg
    23701 tggttttgcc gttggtgccc gttacgccta cgagacgtaa attgtcagag ggttcatggt
    23761 aaaagcggcc cgccagtgca gataaacgct cgttgagctg gctgagatag atgaccggta
    23821 cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcatc tttcgcctct gcaataatgg
    23881 cagccacacc ttgcgctatc gcctgcggga tatatcgacg cccgtccgcc tgatgaccta
    23941 ctacagctac aaagagatcg cccgccgcag ccacacggct gtcgagtgtc atctctcgca
    24001 gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag gtcgcgcaaa ttacgatctg
    24061 ccacctgtcc cctcgccttg attaatcaca aattcatttt tatcgcccgt tgtcagcgca
    24121 tccggctcga tgttcatggt acgcaatacg ccgcccatga tggcaccaaa gaccggcgcg
    24181 gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt tgataacaac aaccagcgcg
    24241 aagcgcggct gactcgcagg cgcaacgcct gcggtataag caatatattt attgatgtag
    24301 cgaccgtccg acccgacctt tttcgcggta ccggttttaa tggcgatacg atagccttta
    24361 atcgccgcct tcacgccgcc gccgcccggt agcgccacgc tttccatcat atgcaccacg
    24421 gtgcggacaa tggattccgg gaagacacgt tcaccgggaa ccggggggtc aactttggta
    24481 atcgacagtg ggcgataaat gccgtagctg ccgatagttg cgtagactcg cgctaactgt
    24541 aacggtgtta ccattagccc gtagccgaaa gagaaggtgg ccctctctat gtcagaccac
    24601 cgttgttttt gaggatataa gccactgcgt tctccgacca accccaaatt ggtcgctttt
    24661 cccagtccaa aacgtgagta agtatctact aacgctgagg acggcatcgc taacgccagc
    24721 ttggaaacac cgacgttact cgacttctgt aataccccgg tcagggttaa ttcgctgtag
    24781 cgtgccacgt ctttgatttc gtggccgtta attcgataag gaatggtatt gagtaccgag
    24841 ttttcccgca ccacgccacg ttgcaacgcg gtcatcacca ccatcggttt aaccgttgag
    24901 cccggttcaa acacgtcggt gatggtacgg ttacgcatcg cctctttcgg cgtgccgctc
    24961 agattgttag ggttgtatga cgggctgtta gccatcgcca gcacttcacc tgtgttgaca
    25021 tccaccagca cggcgctacc agattcagcc ttgttaaagg ccaccgcgtt gttcagttcg
    25081 cgataaacca gcgcctgcag gcgttcatca atactcagcg ccaggttgtg cgctgcctgg
    25141 ctgtcagtag aagaaatatc ttcaattacg cgaccatagc ggtctttacg cacaatgcgc
    25201 tcacccggct gcccggtaag ccatttatcg aaactcttct caacgccctc aatcccttga
    25261 ctatcgacgt tagtaaagcc aatgaggtga gcagtcactt cgccggatgg ataataacgg
    25321 cgagattctt cacgcagatg aatacccggc agtttcagtt ttttgatgta gtccgccatg
    25381 tcagggttta cctgacgcgc cagataaata aagcgccctt tcgggttggc gttaatgcgg
    25441 gcagaaagct gatccagcgg aatattgagc gcgttagcca gcgccttcca gcggtcaccg
    25501 acgctgatac cgccagcgtc atgcacttct ttcgggtcag cccaaatcgc ttttaccggc
    25561 acgctcaccg ctaacgggcg accagaacgg tcagtaatca tgccgcggga ggtggaaact
    25621 tgctgaacgc gaagagaacg catgtcgccc tctttcacca gcatatccgg ggagataact
    25681 tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca ggagaataca gccgcataac
    25741 aacgcaaaac gccaactaat aaagttggca tgttcttcct gacgttttgg tttctgcgtt
    25801 ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt gcactacgat attttcttgt
    25861 gacggatcaa catgctgcat ttgcagcttt tccgtggcga tcctttccac ccggctatgg
    25921 tcgccgagcg cattctcttc aaggatcagg ttgcgccatt caatgtctaa agcatctcgc
    25981 tccagcacca gttgttcgcg ctgagcggtc agtaaacggg tatggtgcgc cgtagttacc
    26041 acggtcaccg ccgtcaaaat aatgcaaatg aacaggcaga gtggcagctt cccaaatcgc
    26101 aaaagatcgt caccgataac accaggcaat gcatggcgct cgtggcttcc catcgatcct
    26161 ttaactttgc ttagagcttc tgtcactctg ctgatcatgc gttcgtcctc tctgcgatac
    26221 gcagaactga actacgggca cgaggattct cagccacctc ttcttcgccc ggcattaact
    26281 tgcctaatgc tcgcagctga cggccaccca gttttttgag ctgctcttca gtcatcggta
    26341 accctgccgg aacttgcgga ccgcggctgt tttcacgcat aaaacgtttc acaatacggt
    26401 cttccagcga gtggaagctg atgatcgaaa gccgcccacc cggggccagc acgttgagcg
    26461 agctttttag cgcctgctct atctcctcca gttcactgtt tacccaaatg cgcaccgcct
    26521 ggaaggtacg ggtcgcggga tgtttaaact tatctttcac cggcgttgca gcagccacga
    26581 cttccgccag ttctttggtg cgggtcatcg gctgttcgcg gttacgctcg acaatggcgc
    26641 aggcaatgcg tttggcaaaa cgctcttcac cataggtttt caatacccag gcgatatcgg
    26701 cttcttctgc ggtttgtagc cattcagcgg ctgactgccc acgggttggg tccatacgca
    26761 tgtccagcgg accatcgcgc ataaaggaaa agccacgttc agcatcatca agttgcggtg
    26821 aagatacgcc aagatcgagg agaatgccgt cgatcttgcc gataagatcg cgctcggcaa
    26881 cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga gaagcgcgga tcatcaatag
    26941 tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc cagcaaacgc ccctcttcgc
    27001 caagctgcga gaggatcaga cgtgagtgac caccgcgacc aaaagtccca tcaatgtaga
    27061 tgccatcagg acggatattg aggccattaa cggcttcatc cagcagcacc gtagtatgtt
    27121 tatagttttc catcatttta tagagacaag tcctgcagtc gctccgataa gtttccagta
    27181 gccaactgct ctgcgtcgat atcttccttg acctgttgat gccaggttgt ttcatcccac
    27241 agctcaaact tgttgaactg tccaaccagc atcacttctt ttgtcagccc ggcatgttgc
    27301 cgcagtactg gcgcgattaa caatcgacct gcgccatcca tctgacattc gctggcatga
    27361 cctaacagta ggcgctgcac acggcgctca actgggttca tgctcgacag acgcgataat
    27421 ttttgctcga taacttccca ttcaggcagg gggtaaagca gcaggcacgg gtgatgaatg
    27481 tcaatggtgc aaaccatttg accggcagcg ttctcaagca gctgttcccg ataacgggta
    27541 ggcactgata agcgcccttt gctgtcgaga ttgactaacg ttgctccccg gaacatgcca
    27601 gcctcacccc ttattcccac tttgccccac aatttcccac tgaaaggagt ttacggagcg
    27661 gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg taaaaaaccc gatgtttacg
    27721 gctaatatca gcgttgttta ttttacgtcc aacgcgcgaa gcaaattaac gttatgaata
    27781 gctgtaagaa aaaatccctc atcgtcatca ctgttttaaa atgactcact attttccaca
    27841 gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt aggacatatc ttccccggtt
    27901 aacagtcctt gttacgtctg tgtggcgctt gatgccagcg ggtttgcgca aaagcaaaga
    27961 aaagtgtttg ttaaatcggc aattcatcgc cgcatgtaac aaaataatgc aaaaacgcgc
    28021 tgtcgatatc acttaattat tgtgaatttc agctggtgag ttaattaacg ggcgggttta
    28081 aaaccattta tcaataatag tcaggaatag tcttatttac tttaagcata ttgatgtcca
    28141 gtcccgtact ctacgcgcca gagggaaatt cacctggcgc gtatttttgt tcgcggctta
    28201 gctacggctg agcacaccgc ggcgatagag attacgttta atgcgcgtta aaccaggttt
    28261 tggcttacgc ggttcgtcta ggcttgccag gacaatctcc agcacacgct ctgcgacatc
    28321 gcggtgacgt tgagccactg ccagcaccgg gcactgtaag aagtcgagca gttcgttatc
    28381 gccaaaggtg gcaattgcca ggtcagaagg cagtttacca tcgcgacgta gcgtgacatc
    28441 catcacccct tgcaacaacg caaacgacgt tgtgaacagc gcctgcggca tcggatgcgt
    28501 ttccagccat ttttcgaata actgggcagc cgcctcccgc tcatagctgt tggcatacag
    28561 gaaatgcact tcgcgcggat catctttcca ggcagtacgg aaaccttgtt cacgcaggaa
    28621 gctgacagaa agctccggca gcgcgccaag ataaagcacc gtctcggcgg gaaacttacg
    28681 taactcttcc gccagcattt cggcatcatc ctgatcggca ccaaccacgc tggtgaaatg
    28741 ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac gggtcgttag cccagcgttg
    28801 ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata atggcatcaa cctgacgctg
    28861 taaaaggtgc tcaatgcagc gcatttcgtt gtctggctga tcttctgagc aggcaatcag
    28921 cagttgataa ccccgttgcc gcgcctggcg ctcaagatag ttagcgatgc gggtatagct
    28981 ggtgttctcc agatcgggga tcacaagacc aatagaacgt gtgcgtccag cacgaagccc
    29041 agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc acagccatga ctttttcaac
    29101 ggttttgtcg ctcacacggt attgcttcgc tttgccgtta ataacatagc ttgcagtggt
    29161 ccgcgacact cccgccagcc gagcgatttc atccagtttc acaattgccc cttgcgtaaa
    29221 atgtaaaaac catagccatt gtactggtat gggttaaatt cattaacatc taagcgcaga
    29281 aaattaactg cggcaactgc ttttattcca ggttcccact gatttcgcaa aaaaagccca
    29341 acgtgacatg ttgggctgca aattgcacac tgagatcatt ctcaacgcat tattttatcg
    29401 ccgcgcgaaa gtccgaccac accagagcga gcaacttcca caattttcgc cacctcgcga
    29461 atcaatgcta aaaatgcatc aagcttatcg ctggtgcctg ctaattgaac ggtataaagc
    29521 gagggggtga catcgataat ttgcccacgg aatatttccg tattacgttt cacttcgtca
    29581 cgcccgtaac cgctggcctg aattttcacc agcatgattt cccgctcaac atgcgcgccc
    29641 tgccccaact cactcacgcg caagacatcg accagcttgt gtaattgctt ttcgatctgc
    29701 tcaagtactt tttcatcgcc cacggtctgg atggtcatac gcgataatgt cggatcgtcg
    29761 gttggcgcaa cggtcaggct ttcaatgttg tagcctcgct gggaaaaaag gccaatcacg
    29821 cgggataacg cgcctgattc attttcgagt aagactgata atatccggcg cataatcagg
    29881 ttctctccgt tttgcttaac cacatttcat ccattccgcc cccgcgaatc tgcatcgggt
    29941 agacgtgctc actgccatcg acggtaacat caacaaacac caggcgatta ttgcgcacct
    30001 gttccagcgc ctcgctaagt ttgctttcca gctcatgcgg atgagagatc tggatcccga
    30061 cgtgaccata ggcttccgcc agacggacga aatcgggtag tgattgcata taagattgtg
    30121 aatgacggcc ggaatagatc atgtcctgcc actgtttcac catccccaga tagcggttat
    30181 tgagattcac caccagtacg ggcaactcgt attgcaacgc ggtagacagt tcctgaatgt
    30241 tcatctgaat actgccgtcg ccagtgacgc aaaccacggt ttcttctggc aacgccattt
    30301 tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc gaggccaccg gaattgatcc
    30361 agcgacgcgg tttgtcgaat ggataataaa gtgcagcaaa catctggtgc tgcccgacat
    30421 cggacgtcac gtaagcgtct cccttcgtca accgccaaag agtctcgatc accgcctgcg
    30481 gtttaatctt ttcactgtga gtgtcatatt tcaggcactg acgagcgcgc cactgttcaa
    30541 tttgctgcca ccagtcgcgg atctcatcca gtggttgatg ggcggattct tgcgacaaga
    30601 gttcaagcat ttgttcgagg acctggcgag catcccccac aatcgggata tccgcagtca
    30661 cggttttaga aatggaagta ggatcaatat cgatatgcag aacagtggca tttgggcagt
    30721 actttgccag attgttcgtc gttcggtcat caaatcgtac cccgacggcg aaaatcacat
    30781 ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg cattcccagc atgcccagtg
    30841 cctgacgatg cgttgccgga aacgccccca gccccatcaa tgaggaaaca acgggcagat
    30901 tcaacgcctc caccgtttct ttcaactgct gatggcagcc cgccatgatt gccccaccgc
    30961 ctacgtagac aaccggtttt tttgccacta ccagcgtttg cagagcacgc ttaatttgcc
    31021 ctttatgtcc ggtagtagtg ggattgtaag aacgcatact gaccgactcc ggccagacat
    31081 agggtaattt gttcgccgga ttaagaatat ctttcggtaa atcaacgact actggtccag
    31141 gacgaccact tgccgccagc cagaaagcct ttttcagcac ctgcggaatg tcttccgttt
    31201 gcttaaccag aaaactgtgt ttaaccactg gtcgcgaaat ccccaccatg tcgcactcct
    31261 gaaaggcatc gtaacctatc aacgaggtcg ctacctgccc ggaaaggaca actaatggaa
    31321 tggaatccat ataagcggtg gcgatgccgg taatcgcatt ggtcgcccct ggacccgacg
    31381 ttaccagcac gacgccgact tccccggtcg cgcgcgccag accatcggcc atatgcactg
    31441 ccgcctgctc atgacgaact aaaacatgat cgataccacc cacggtatgc aatgcatcgt
    31501 aaatatcaag gactgcgcct ccaggataac cgaatacttg tttaacgccc tgatcgataa
    31561 gcgatcggac gaccatctcg gctccagaca acatctccat ggcctgcctc actgtttgat
    31621 ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct taagcaattg gcagccagag
    31681 acataaacgc agggtaataa gaataaacag gaggaaaggt gaaaagaaac gataaaatcc
    31741 tccattacga aggattaaat tgataataaa tcggctgaat cccacaactt acagcaattt
    31801 ctctattttt ttaattaatc ctaaaataga gagcccacac attcagaata aaattctaca
    31861 ttcaataaaa aatgcagaat aaaccagaaa ttcatgtctg acctattctg cattcagtta
    31921 gcgtttgcaa attgagacta attgttcttc catccactga tggcctttat cgcgcccggc
    31981 agcttcatgc catgagagat aacaggttct gctgttttgt tttaacggca gcggtaatac
    32041 ctgtaattct aaggattcag cgaactcttc agccagccaa cgcggcgcaa tagcgaccaa
    32101 atgcgtttgc gacaccacgc taagtacgct catcattgcc atgccctgat acgcgatact
    32161 ggcttgctta tctaccgtgt cataccaagg ttgactaaat gacgcgaaac gatcgagcga
    32221 aaccgccgca tgttgttcgt tataaacatc atgtttcagt aacgggcctt taattgttgg
    32281 atgatttttg ctggctacca gcaccatttc atctttaaat aatggcacgc tggtaaattc
    32341 aggacgatgg aagtcttcat aactaatcac aaactccgtt tcctgataac gcagctgatg
    32401 ttcagtgttc tgatttaatg aagacttgaa cataacatgt atatttggcg caatctgctc
    32461 aatgtgatta taaatctgcg aggtcagaat gctgtctaac gggctgcaaa cacaaagatg
    32521 aaatacacgt tcactactcg cgggttcaaa acctgaacca ggcaattcat tttgtactag
    32581 ttgcaatgcc tggcgaactg aaccaaaaag ttgaaatgcg cgagcagtcg gttgaatacc
    32641 acggccataa cgaacaaaaa gctcgtcatt aaacatcacc ttcaggcgtg caacagcgtt
    32701 actgaccgca ggttgcgaca tacccagaac atgagcggca cgggtaatgt tttgctcctg
    32761 catcacggca tcgaaaacgg ttaataagtt gagatcgacc atgcgtagct gtggcttgct
    32821 taactccacc gtctctgaat gatctgtttg tacctctggc atacttaact ccactgtcac
    32881 acttaactcc ctttccctta ttggaatgca gagaaataat cctgaagaat atgatttatc
    32941 atgcatataa aataagaaaa agcaaaatga gtaaaattcg gaaaacataa agatatgggt
    33001 tctgataaaa acagaatcca ttgccgtgga atgagtcatt taccacagca taataatcca
    33061 taatgttttt gttgcgaaaa caatctaatc ataactaccg cgaatactca atcatctaca
    33121 aaatggatta aatgtgctta tttaataatt aatttatata tttaatgcat taattattaa
    33181 cattaatgga tcaataatat tcaccaaatc aatatcaaaa aaaattcgca aaacatataa
    33241 ttcaatacaa atcatcagga taggttttgc aacgcgtgca ttttgtcccc tttttcctcg
    33301 ttgattagat gcaaaaattt attctgaaat atgtcaacct atgaaaagtg tcggtcgtta
    33361 aacagaaatt aatatcgctt gctttaacca ccgcagcaca attagctaat tttacggatg
    33421 cagaactcgc gctggcggga cgtttttatt gcgtcagggt tgacatccgt ttttgtatcc
    33481 agtaactcta aaagcatatc gcattcatct ggagctgatt taatgactca catcgttcgc
    33541 tttatcggtc tactactact aaacgcatct tctttgcgcg gtagacgagt gagcggcatc
    33601 cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc cattgcgcgg gtttttttat
    33661 gcccgaagcg aggcgctcta aaagagacaa ggacccaaac catgagccag caagtcatta
    33721 ttttcgatac cacattgcgc gacggtgaac aggcgttaca ggcaagcttg agtgtgaaag
    33781 aaaaactgca aattgcgctg gcccttgagc gtatgggtgt tgacgtgatg gaagtcggtt
    33841 tccccgtctc ttcgccgggc gattttgaat cagtgcaaac catcgcccgc caggttaaaa
    33901 acagccgcgt atgcgcgtta gctcgctgcg tggagaaaga tatcgacgtg gcggccgaat
    33961 ccctgaaagt cgccgaagcc ttccgtattc atacctttat tgccacttcg ccaatgcaca
    34021 tcgccaccaa gctgcgcagc acgctggatg aagtgatcga acgcgctatc tatatggtga
    34081 aacgcgcccg taattacacc gatgatgttg aattttcttg cgaagatgcc gggcgtaccc
    34141 ccattgccga tctggcgcga gtggtcgaag cggcgattaa tgccggtgcc accaccatca
    34201 acattccgga caccgtgggc tacaccatgc cgtttgagtt cgccggaatc atcagcggtc
    34261 tgtatgaacg cgtacctaac atcgacaaag ccattatttc cgtacatacc cacgacgatt
    34321 taggcctggc ggtcggcaac tcactggcgg cggtacatgc cggtgcacgc caggtggaag
    34381 gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc gctggaagaa gtcatcatgg
    34441 cgattaaagt tcgtaaggat attctcaacg tccacaccgc cattaatcac caggagatat
    34501 ggcgcaccag ccagttagtt agccagattt gtaatatgcc gatcccggca aacaaagcca
    34561 ttgttggcag cggcgcattc gctcactcct ccggtatcca ccaggatggc gtgctgaaaa
    34621 accgcgaaaa ctacgaaatc atgacaccag aatctattgg tctgaaccaa atccagctga
    34681 atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg catggatgag atggggtata
    34741 aagaaagtga atataattta gacaatttgt acgatgcttt cctgaagctg gcggacaaaa
    34801 aaggtcaggt gtttgattac gatctggagg cgctggcctt catcggtaag cagcaagaag
    34861 agccggagca tttccgtctg gattacttca gcgtgcagtc tggctctaac gatatcgcca
    34921 ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc agaagccgcc aacggtaacg
    34981 gtccggtcga tgccgtctat caggcaatta accgcatcac tgactataac gtcgaactgg
    35041 tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc gctgggtcag gtggatgttg
    35101 tcgccaacta caacggtcgc cgcttccacg gcgtcggcct ggccaccgat attgtcgagt
    35161 cctccgccaa agccatggtg cacgtactga acaatatctg gcgtgccgca gaagtcgaaa
    35221 aagagttgca acgcaaagct caacacaacg aaaacaacaa ggaaaccgtg tgatgtcgaa
    35281 gaattaccat attgccgtat tgccggggga cggtattggt ccggaagtga tgacccaggc
    35341 gctgaaagtg ctggatgccg tgcgcaaccg ctttgcgatg cgcatcacca ccagccatta
    35401 cgatgtaggc ggcgcagcca ttgataacca cgggcaacca ctgccgcctg cgacggttga
    35461 aggttgtgag caagccgatg ccgtgctgtt tggctcggta ggcggtccga aatgggaaca
    35521 tttaccacca gaccagcaac cagaacgcgg cgcgctgttg cctttgcgta agcacttcaa
    35581 attattcagc aacctgcgtc cggcaaaact gtatcagggg ctggaagcat tctgtccgct
    35641 gcgtgctgac attgccgcta acggcttcga catcctgtgc gtgcgcgaac tgaccggcgg
    35701 catctatttc ggtcagccaa aaggccgcga aggtagcgga cagtatgaaa aagcgtttga
    35761 taccgaggtg tatcaccgtt ttgagatcga acgtatcgcc cgcatcgcgt ttgaatctgc
    35821 ccgcaagcgt cgccacaaag tcacctcaat cgacaaagcc aacgtgctgc aatcctctat
    35881 tttatggcgg gagatcgtta acgagatcgc cacggaatac ccggatgtcg aactggcgca
    35941 tatgtacatc gacaacgcca ccatgcagct gattaaagat ccatcacagt ttgacgtcct
    36001 gctgtgctcc aacctgtttg gcgacattct gtctgacgag tgcgcaatga tcactggctc
    36061 gatggggatg ttgccttccg ccagcctgaa cgagcaaggt tttggtctgt atgaaccggc
    36121 aggcggctca gcaccagata tcacaggcaa aaacatcgcc aacccgattg cgcaaattct
    36181 gtcgctggca ctgctgctgc gctacagcct ggatgccgat gatgcggctt acgccattga
    36241 acgcgccatt aaccgcgcat tagaagaagg cattcgcacc ggggatttag cccgtggcgc
    36301 tgccgccgtt agtaccgatg aaatgggcga tatcattgcc cgctatgtgg cagaaggggt
    36361 gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg ctcacgtagt gtacgaagcc
    36421 gaaaacgaaa ccccgctgtt atatatcgac cgccacctgg tgcatgaagt gacctcaccg
    36481 caggcgttcg atggtctgcg cgcccacggt cgcccggtac gtcagccggg caaaaccttc
    36541 gccactatgg atcacaacgt ctctacccag acgaaagaca ttaatgcctg tggtgaaatg
    36601 gcgcgcatcc agatgcagga gctgattaaa aactgcaaag aatttggcgt cgaactgtat
    36661 gacctgaatc acccgtatca ggggatcgtc cacgtaatgg ggccggaaca gggcgtcacc
    36721 ttgccgggga tgaccattgt ctgcggcgac tcgcataccg ccacccacgg cgcgtttggc
    36781 gcactggcct ttggtatcgg cacttccgaa gttgaacacg tactggcaac gcaaaccctg
    36841 aaacagggcc gcgcaaaaac catgaaaatt gaagtccagg gcaaagccgc gccgggcatt
    36901 accgcaaaag atatcgtgct ggcaattatc ggtaaaaccg gtagcgcagg cggcaccggg
    36961 catgtggtgg agttttgcgg cgaagcaatc cgtgatttaa gcatggaagg tcgtatgacc
    37021 ctgtgcaata tggcaatcga aatgggcgca aaagccggtc tggttgcacc ggacgaaacc
    37081 acctttaact atgtcaaagg ccgtttgcat gcgccgaaag gcaaagattt cgacgacgcc
    37141 gttgcctact ggaaaaccct gcaaaccgac gaagtcgcaa ctttcgatac cgttgtcact
    37201 ctgcaagcag aagaaatttc accgcaggtc acctggggca ccaatccagg ccaggtgatt
    37261 tccgtgaacg acaatattcc cgatccggct tcgtttgccg atccggttga acgcgcgtcg
    37321 gcagaaaaag cgctggccta tatggggctg aaaccgggca ttccgctgac cgaagtggct
    37381 atcgacaaag tgtttatcgg ttcctgtacc aactcgcgca ttgaagattt acgcgcggca
    37441 gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc aggcactggt ggttcccggc
    37501 tctggcccgg taaaagccca ggcggaagcg gaaggtctgg ataaaatctt tattgaagcc
    37561 ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg cgatgaacaa cgaccgtctg
    37621 aatccgggcg aacgttgtgc atccaccagc aaccgtaact ttgaaggccg ccaggggcgc
    37681 ggcgggcgca cgcatctggt cagcccggca atggctgccg ctgctgctgt gaccggacat
    37741 ttcgccgaca ttcgcaacat taaataagga gcagaccatg gcagagaaat ttatcaaaca
    37801 cacaggcctg gtggttccgc tggatgccgc caatgtcgat accgatgcaa tcatcccgaa
    37861 acagtttttg cagaaagtga cccgtacggg ttttggcgcg catctgttta acgactggcg
    37921 ttttctcgat gaaaaaggcc aacagccaaa cccggacttc gtgctgaact tcccgcagta
    37981 tcagggcgct tccattttgc tggcacgaga aaacttcggc tgtggctcct cgcgtgagca
    38041 cgcgccctgg gcattgaccg actacggttt taaagtggtg attgcgccga gttttgctga
    38101 catcttctac ggcaatagct ttaacaacca gctgctgccg gtgaaattaa gcgatgcaga
    38161 agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg atccatttcg acgtggatct
    38221 ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc tttaccatcg atgccttccg
    38281 ccgccactgc atgatgaacg gtctggacag tattgggctt accttgcagc acgacgacgc
    38341 cattgccgct tatgaagcaa aacaacctgc gtttatgaat taatcccctt gcccggtcaa
    38401 atgaccgggc tttccgctat cgtccacgtc atcaaacgtc tttaaccttt gcggttaaaa
    38461 ataatgcgac aacagaaata accgcaatta cccagtagac agcaaagtgc ccccaacttt
    38521 gtgcaattgc tccctgaata acgccagcca gaattacccc ggtagaaata ctgttagtaa
    38581 ataaggtggt agctgccccc gctcttccag gcattaaatc ctgaaaccat agcatcccaa
    38641 tacccgcaac aatgccgata aatacagcgt taaaaagttg cagcgtcatc aacgccatac
    38701 ggctatgaaa gaaaatcaat ccggtgtaaa acagtactcc tgccgccact gctatgacca
    38761 tcattcgccg cttaccataa cgtttgacat agtagccagc cagaatcatt gctggtattt
    38821 ccagtccagc tgccgtcccc atcaggaaac ccgccagttt gtctggcaat cctaactcgc
    38881 tactgatcca caacggcata tcaataatgt acatggtgtt gcaggtccac attaacgtcg
    38941 aggcgacaaa taacatccgt acgttactat cctgccagcc accttgcatt gataaagcat
    39001 tttccgacgg cagttctacc cgcgccacag acggaagcat aaatgcaatc aataccagac
    39061 tgagtgtgaa tatcccggcg gcaatcgaaa acatcaccac ttctcgcgcc gagttatccg
    39121 catattcccg cgccagagca aataactgtg gcattgccgt attggccaga gatgccagaa
    39181 gcacaccaca ggtgataagc gtcagataat gacgattaaa tgcaaacaat ggcgcattgc
    39241 cgatagccat caaacagcaa aatataatca gttttcgccg atcgccctga ctgtctgaac
    39301 gttttgccaa ccagaggctt acgccgatcc cagcaatagt attcaccgta taaaagagtc
    39361 cgatccagaa aggttgcgcg ccaacctcac gactcagaaa taagctcaat gtaggagcct
    39421 gtagcgcccc ggccccccct atcataaaag cgaccagcat aaatgccgcg taaacaccgt
    39481 tcatacggcg agccatcgtc attatccaga tcatacgttc cctttttagc gcggcgagaa
    39541 taaaaaaaac cagcaggtat aatctgctgg cgggtgattt tacaccaata ctcagtcaca
    39601 catgatgcag gcaagtcaac tttcagaatt gcggtcatcc cactgcatca gtccttcaag
    39661 catggttaat cgttgtggga cgcttaacca acgcaaccag cacaacttcg ctgtcgcggt
    39721 aaaatagtgc tgataaaact gacgcatggg gcacctcctt gcttcatcgt taggaattat
    39781 tgacttaata tagggaaaat aaagttgctg tcttttgcgc aggagttccc cttttatgtc
    39841 atctgctcgt ctgcaacaac agttcatccg cctgtggcaa tgctgcgagg gtaaatcgca
    39901 ggacacaacg ctcaacgaac tggcagcgtt attgagctgc tcgcgtcgtc atatgcgtac
    39961 cctgctcaac accatgcagg atcgcggctg gctgacgtgg gaagcggaag tcgggcgcgg
    40021 taaacgctcg cgtctgacat tcctctatac cgggctggcg cttcagcaac agcgggcgga
    40081 agacctgctg gagcaggatc gtatcgacca actagtgcag ttggttggcg acaaagcgac
    40141 tgtgcggcaa atgctggttt ctcatctggg ccgcagcttc cgccaggggc ggcacatcct
    40201 gcgcgtgctc tactatcgtc cgttgcgtaa tctgctacct ggcagcgcat tgcgccgttc
    40261 cgaaacccat attgcccggc aaatcttcag ttcgctaacg cgcataaatg aggaaaatgg
    40321 ggaactggaa gcagacatcg cccaccactg gcagcaaata tcaccgcttc actggcgttt
    40381 ctttttgcgt ccaggagtcc attttcatca tggtcgtgaa ctggaaatgg acgacgtgat
    40441 cgcctcttta aaacgaatca atacgctgcc gctctattcg catattgcgg acattgtgtc
    40501 gccgacgccc tggacgctgg atatccatct cacgcaaccg gaccgctggt taccgttact
    40561 gctggggcaa gttccggcga tgatcctgcc gcgcgaatgg gaaaccctca gtaactttgc
    40621 cagccatccc atcggcaccg gtccgtatgc ggtgatccgc aacaccacca atcaactgaa
    40681 aattcaggca ttcgatgact tcttcggtta ccgggcatta atcgatgaag ttaacgtctg
    40741 ggttctgccg gaaattgccg acgagccagc tggaggtctg atgctaaaag gtccacaggg
    40801 tgaggaaaaa gagattgaaa gccgcctgga ggaaggttgc tactatttac tattcgacag
    40861 ccgcacccat cgcggagcga atcagcaagt cagggactgg gtaagctatg tgctttctcc
    40921 aactaatctg gtctatttcg ctgaggaaca gtaccagcaa ctgtggttcc cggcttatgg
    40981 actgctcccc cgttggcacc atgcccgcac cataaagagc gaaaaaccgg ctggcctgga
    41041 aagcctcacc ctgacctttt atcaggatca cagtgagcat cgggtgattg ccgggatcat
    41101 gcagcagatt ctggcaagtc accaggtcac gctggaaatc aaagagatca gctacgatca
    41161 gtggcatgaa ggagagatcg agagcgatat ctggcttaat agcgccaact ttacgctgcc
    41221 gctggatttt tcgctgttcg cgcacttgtg cgaggtgccg ctgctccaac actgtattcc
    41281 gatcgactgg caagccgacg ccgcccgctg gcgcaatggc gaaatgaacc tggcgaactg
    41341 gtgccagcaa ctggtcgcca gtaaagcaat ggtgccgctt atccaccact ggctgatcat
    41401 tcagggacaa cgcagtatgc gcggcctgcg tatgaatacc ctcggctggt tcgattttaa
    41461 atcagcgtgg tttgcgccgc cggatccatg attgctggta gataacaaaa tcactacact
    41521 aacgccgttc tcaacggggt gccacgcaaa cgcgtgcgct gagaaaatac ccgtcgaacc
    41581 tgatccggat aacgccggcg aagggatttg aggctccttc tcaagtcctt tgccactctt
    41641 ttttgaggtg caaagtgtta aaaaaatgtc ttcccctgct gttgctgtgc acagcgcccg
    41701 ttttcgctaa acccgttctg actgtttata cctacgattc cttcgccgcc gactgggggc
    41761 ctggtccaaa gatcaaaaaa gcttttgaag ccgactgtaa ctgcgaactg aaactggtgg
    41821 cgctggaaga tggcgtttcg cttctcaacc gtctacggat ggaaggcaaa aatagtaaag
    41881 ccgatgtggt gctggggctg gataacaatc tgttagacgc cgccagtaaa accggactat
    41941 ttgccaaaag cggtgtggca gcggatgccg ttaacgttcc cggcggctgg aataatgaca
    42001 ctttcgtcca gtttgattac ggctatttcg ccttcgtcta tgacaagaac aaactgaaaa
    42061 acccgccgca aagcctgaaa gaattggttg agagcgatca aaactggcgg gtgatttatc
    42121 aggatccgcg caccagtacg ccggggctgg gcctgctgtt gtggatgcaa aaagtctatg
    42181 gcgatgacgc cccacaagcc tggcaaaaac tggcgaagaa aaccgtcacg gtcaccaaag
    42241 gctggagcga agcctacggc ctgtttttaa aaggtgaaag cgatctggta ctgagttaca
    42301 ccacctctcc ggcttatcac attctcgaag agaaaaaaga taactacgcc gccgcgaact
    42361 ttagcgaagg tcactatctg caggtggaag tcgccgcccg caccgctgcc agcaagcagc
    42421 cggagctggc gcaaaaattc ctccagttta tggtttctcc ggcttttcag aatgcgatcc
    42481 caaccggtaa ctggatgtat ccggtggcaa atgtcacgct gcctgccggg tttgaacaat
    42541 tgaccaaacc ggccaccacg ctggagttca caccagctga agtggcggca caacgtcagg
    42601 catggattag cggatggcaa cgcgccgtca gccgttaatt cccggctggt taattccagg
    42661 tgtaagcgcc gccacgctgg tggtagcggt tgcgctcgcg gcgtttctcg ccctgtggtg
    42721 gaacgcgccg cagggtaact gggtggcagt ctggcaggac agctacctgt ggcatgtggt
    42781 gcgcttctcc ttctggcagg cgtttctctc ggcgctactc tctgtcgtac ccgcgatatt
    42841 cctcgcccgc gccctctatc gcaggcgctt tccgggtcgg ctggcgctgt tgcgtctgtg
    42901 cgcaatgacc ttgatcctcc cggtgctggt tgctgttttc ggcattctta gcgtctatgg
    42961 tcgccagggc tggctggcat cgctctgcca atcgctcggt ctggagtgga ccttttcgcc
    43021 ctacggcctg caaggtattt tgctggcgca cgtatttttt aatctgccga tggcgagccg
    43081 cttattactc caggcactgg aaaacattcc cgacgaacaa cgtcagcttg ccgcccagct
    43141 tgggatgcgc ggctggcatt ttttccgctt cgtcgaatgg ccgtggttac ggcgacaaat
    43201 cccgccggtt gctgcactta tctttatgct ctgtttcgcc agcttcgcca ccgtgctatc
    43261 actgggcggc ggtccgcagg cgaccactat cgagctggct atttatcagg cgctgagtta
    43321 cgactacgat cctgcccgcg cggcgatgct ggcgctgatc cagatggtgt gttgccttgg
    43381 gctggtactg ttgagtcagc gattgagtaa ggccattgcg ccaggcacca cgctgctgca
    43441 aggctggcgc gacccggacg atcgtctgca tagccgcatt tgcgacacgg tgttaatagt
    43501 gctggcgctg ctgctgttgt tgccaccgtt gctggcggtg atcgtcgatg gggtaaatcg
    43561 ccagttgccg gaagtgctgg cacaaccggt gctgtggcag gcgctgtgga cctcgttgcg
    43621 tattgcgctg gcggcaggtg tattgtgcgt agtgctgacc atgatgctgc tatggagcag
    43681 tcgcgaactt cgggcgcggc agaaaatgct ggcgggtcag gcgctggaga tgagcggcat
    43741 gttgattctc gccatgccgg ggattgtgct ggctaccggc ttctttttac tgctcaacaa
    43801 cactatcggc ctgcaacaat ctgctgacgg cattgtgatt ttcaccaatg cgttaatggc
    43861 gatcccttat gcgctgaaag tactggaaaa cccgatgcgc gatatcaccg cccgctacag
    43921 tatgttgtgt cagtcgctgg gcattgaagg ctggtcgcgc ttaaaagtgg tggagctgcg
    43981 cgccctgaaa cgtccactgg cgcaggcgct ggcctttgcc tgcgtgctgt cgattggtga
    44041 ttttggcgtg gtggcgttgt tcggtaacga tgatttccgt accctgccgt tttatctcta
    44101 ccagcaaatt ggctcctatc gcagccagga cggcgcggtc accgcgttaa ttctgctgct
    44161 actctgtttt ctgctgttta ccgtgattga aaaactaccg gggcgaaatg ttaaaactga
    44221 ctgatatcac ctggctttac caacatttgc cgatgcgttt tagcttaacg gtggaacgcg
    44281 gcgagcaggt ggcgatcctc gggccaagcg gcgcaggtaa aagtaccctg ctgaatttga
    44341 ttgccggttt tctgacgcca gccagcggtt cgctgactat cgatggcgtt gatcacacca
    44401 cgacgccgcc gtcacgccgt ccggtgtcga tgctgtttca ggagaacaac ctgttcagcc
    44461 acctgacggt cgcgcagaac atcgggctgg ggcttaatcc aggattgaag ctgaacgcgg
    44521 cacagcagga gaaaatgcac gctatcgccc gtcagatggg gattgataat ttaatggcgc
    44581 ggttaccggg cgagctttcc ggcggccagc gacagcgcgt ggcgttagcg cgttgtctgg
    44641 tgcgcgaaca gccgatttta ttgctcgatg aaccgttctc tgcgctcgat ccggcgttac
    44701 gtcaggagat gctgacgctg gtgagcacga gctgccagca gcaaaaaatg acgctattga
    44761 tggtgtcgca cagtgtggaa gatgcggcgc ggatcgccac acgctcggtg gtggtcgccg
    44821 acgggcgcat cgcctggcag ggtaagactg aagaattgtt gagcggtaag gcgagtgctt
    44881 cggcaatatt ggggattacg ggttagtatg cactgtcaga tgcggcgtga acaccttatc
    44941 cgacctacgg acagtgcgtt ttgtaggcct gataagacgc ggtcagcgtc gcatcaggca
    45001 acccgcacaa gacaatcccc taaaccccaa ccactttacg caaaatatcg atatacaccg
    45061 gcatcaacgg gtggcgaatt aacaccacca gcgccaccac gccgatggca gaaatcaacg
    45121 gcgtcagcca caacaaacgg ccgcgcgaca aataatgact taaacgatcg gttgctttac
    45181 cgctgcgcca taaccgccag cacagccagc caccaaccca caaaaatacc gccgtcgcca
    45241 gcagcaacca tttaaactcg ccgctctgca ttccggctgg aatatcgatc gccgcgcccg
    45301 ccagaatccc aggcaggaag taaaacggtg gccacagcag gcagccgata atattcggcg
    45361 taatgaattt tgccaccggc agatccagca ttcccgccac cattggcacc agcggacgcg
    45421 tcgggccaac aaagcgaccg accagaatgg tgaacatgct gtgttgatgc aacgcatgtt
    45481 cagttttatc gagtaatgct ttgtttttct tcagaaatga ccagcgatgc aacggctttt
    45541 taaaacgcca gcccagccag aaggagatcc agtcgcccag cagacaaccg acaatccctg
    45601 ccagccaggc gtgccagaaa cttaactcgc cgctgccaat cagcgctccc agccccgcca
    45661 tcagcaccgt accgggtaga atcaaaccga ccagcgccag cgactccaga aaggccacca
    45721 acaccaccgc catcaatgaa tacacggtag attgggtaat aaagtgttcc agcaatgctt
    45781 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
    45841 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
    45901 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
    45961 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
    46021 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
    46081 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
    46141 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
    46201 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
    46261 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
    46321 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
    46381 tccgggcgaa agaaacccgt attgacaaat attgacggcc agttaagcca ttcatgccag
    46441 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
    46501 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
    46561 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
    46621 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
    46681 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
    46741 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
    46801 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taacccaacc ggtaaccccg
    46861 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
    46921 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
    46981 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
    47041 ctctctactg tttctccata cccgtttttt ggatggagtg aaacgatggc gattgcaatt
    47101 ggcctcgatt ttggcagtga ttctgtgcga gctttggcgg tggactgcgc cagcggtgaa
    47161 gagatcgcca ccagcgtaga gtggtatccc cgttggcaaa aagggcaatt ttgtgatgcc
    47221 ccgaataacc agttccgtca tcatccgcgt gactacattg agtcaatgga agcggcactg
    47281 aaaaccgtgc ttgcagagct tagcctcgaa cagcgcgcag ctgtggtcgg gattggcgtt
    47341 gacagtaccg gctcgacgcc cgcaccgatt gatgccgacg gtaacgtgct ggcgctgcgc
    47401 ccggagtttg ccgaaaaccc aaacgcgatg ttcgtattgt ggaaagacca cactgcggtt
    47461 gaagaagcgg aagagattac ccgtttgtgc cacgcgccgg gcaacgttga ctactcccgc
    47521 tacattggtg gtatttattc cagcgaatgg ttctgggcaa aaatcctgca tgtgactcgc
    47581 caggacagcg ccgtggcgca atctgccgca tcgtggattg agctgtgcga ctgggtgcca
    47641 gctctgcttt ccggtaccac ccgcccgcag gatattcgtc gcggacgttg cagcgccggg
    47701 cataaatctc tgtggcacga aagctggggc ggcttgccgc cagccagttt ctttgatgag
    47761 ctggacccga tcctcaatcg ccatttgcct tccccgctgt tcactgacac ctggactgcc
    47821 gatattccgg tgggcacctt atgcccggaa tgggcgcagc gtctcggcct gcctgaaagc
    47881 gtggtgattt ccggcggcgc gtttgactgc catatgggcg cagttggcgc aggcgcacag
    47941 cctaacgcac tggtaaaagt tatcggtact tccacctgcg acattctgat tgccgacaaa
    48001 cagagcgttg gcgagcgggc agttaaaggt atttgcggtc aggttgatgg cagcgtggta
    48061 cctggattta tcggtctgga agcaggccaa tcggcgtttg gtgatatcta cgcctggttt
    48121 ggtcgcgtac tcggctggcc gctggaacag cttgccgccc agcatccgga actgaaagcg
    48181 caaatcaacg ccagccagaa acaactgctt ccggcgctga ccgaagcatg ggccaaaaat
    48241 ccgtctctgg atcacctgcc ggtggtgctc gactggttta acggccgccg cacaccgaac
    48301 gctaaccaac gcctgaaagg ggtgattacc gatcttaacc tcgctaccga cgctccgctg
    48361 ctgtttggcg gtttgattgc tgccaccgcc tttggcgcac gcgcaatcat ggagtgcttt
    48421 accgatcagg ggatcgccgt caataacgtg atggcgctgg gcggcatcgc gcggaaaaac
    48481 caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc cgctgcaaat tgttgcctct
    48541 gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg tcgccgcgaa agtgcacgca
    48601 gacatcccat cagcccagca aaaaatggcc agtgcggtag agaaaaccct gcaaccgcgc
    48661 agcgaacagg cacaacgctt tgaacagctt tatcgccgct atcagcaatg ggcgatgagc
    48721 gccgaacaac actatcttcc aacttccgcc ccggcacagg ctgcccaggc cgttccgact
    48781 ctataaggac acgataatga cgatttttga taattatgac gtgtggtttg tcattggcag
    48841 ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc caacatgccg agcacgttgt
    48901 taatgcgctg aatacggaag cgaaactgcc ctgcaaactg gtgttgaaac cgctgggcac
    48961 cacgccggat gaaatcaccg ctatttgccg cgacgcgaat tacgacgatc gttgcgctgg
    49021 tctggtggtg tggctgcaca ccttctcccc ggccaaaatg tggatcaacg gcctgaccat
    49081 gctcaacaaa ccgttgctgc aattccacac ccagtttaac gcggcgctgc cgtgggacag
    49141 tatcgatatg gactttatga acctgaacca gactgcacat ggcggtcgcg agttcggctt
    49201 cattggcgcg cgtatgcgtc agcaacatgc cgtggttacc ggtcactggc aggataaaca
    49261 agcccatgag cgtatcggct cctggatgcg tcaggcggtc tctaaacagg atacccgtca
    49321 tctgaaagtc tgccgttttg gcgataacat gcgtgaagta gcggtcaccg atggtgataa
    49381 agttgccgca cagatcaagt tcggtttctc cgtcaatacc tgggcggttg gcgatctggt
    49441 gcaggtggtg aactccatca gcgacggcga tgttaacgcg ctggtcgatg agtacgaaag
    49501 ctgctacacc atgacgcctg ccacacaaat ccacggcgaa aaacgacaga acgtgctgga
    49561 agcggcgcgt attgagctgg ggatgaagcg tttcctggaa caaggtggct tccacgcgtt
    49621 caccaccacc tttgaagatt tgcacggtct gaaacagctt ccaggtctgg ccgtacagcg
    49681 tctgatgcag cagggttacg gctttgcggg cgaaggcgac tggaaaaccg ccgccctgct
    49741 tcgcatcatg aaggtgatgt caaccggtct gcagggcggc acctccttta tggaggacta
    49801 cacctatcac ttcgagaaag gtaatgacct ggtgctcggc tcccatatgc tggaagtctg
    49861 cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt cagcatctcg gtattggtgg
    49921 taaggacgat cctgcccgac tgatcttcaa tacccaaacc ggtccagcga ttgtcgccag
    49981 cctgattgat ctcggcgatc gttaccgtct gctggttaac tgtatagaca cggtgaaaac
    50041 accgcactcc ctgccgaaac tgccggtggc gaatgcgctg tggaaagcgc aaccggatct
    50101 gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg caccataccg tcttcagcca
    50161 tgcgctgaac ctcaacgata tgcgtcagtt cgccgagatg cacgacattg aaatcacggt
    50221 gattgataac gacacccgcc tgccagcgtt taaagacgca ctgcgctgga acgaagtgta
    50281 ttacggattt cgtcgctaag tagccacatc aggtatgtaa cgcctgatgc gacgctgacg
    50341 cgtcttatct ggcctacacg ctgcgatttt gtaggccgga taagcaaagc gcatccggca
    50401 ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct acgcgctgcg attttgtaga
    50461 ccggataagc gaagcgcatc cggcacgaag gagtcaacat gttagaagat ctcaaacgcc
    50521 aggtattaga agccaacctg gcgctgccaa aacacaacct ggtcacgctc acatggggca
    50581 acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt
    50641 acagcgtcat gaccgctgac gatatggtcg tggttagcat cgaaaccggt gaagtggttg
    50701 aaggtacgaa aaagccctcc tccgacacgc caactcaccg gctgctctat caggcattcc
    50761 cctccattgg cggcattgtg catacgcact cgcgccacgc caccatctgg gcgcagtcgg
    50821 gtcagtcgat tccagcaacc ggcaccaccc acgccgacta tttctacggc accattccct
    50881 gcacccgcaa aatgaccgac gcagaaatca acggcgaata tgagtgggaa accggtaacg
    50941 tcatcgtaga aacctttgaa aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg
    51001 tccattccca cggcccgttt gcatggggca aaaatgccga agatgcggtg cataacgcca
    51061 tcgtgctgga agaggtcgct tatatggaga tattctgccg tcagttagcg ccgcagttac
    51121 cggatatgca gcaaacgctg ctggataaac actatctgcg taagcatggc gcgaaggcat
    51181 attacgggca gtaatgactg tataaaacca cagccaatca aacgaaacca ggctatactc
    51241 aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca ggttttatct taacccgaca
    51301 ctggcgggac accccgcaag ggacagaagt ctccttctgg ctggcgacgg acaacgggcc
    51361 gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt attcccgccg atcaggttcc
    51421 ccgcgctcag catattttgc agggtgaaca aggctttcgc ctgacaccgc tggcgttaaa
    51481 ggattttcac cgccagccgg tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa
    51541 ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc
    51601 agaacgctat ctgatggagc ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca
    51661 caatggcact atcgttaatg cccgtctgaa accgcatccc gactatcgtc cgccgctcaa
    51721 gtgggtttct atagatattg aaaccacccg ccacggtgag ctgtactgca tcggcctgga
    51781 aggctgcggg cagcgcatcg tttatatgct ggggccggag aatggcgacg cctcctcgct
    51841 tgatttcgaa ctggaatacg tcgccagccg cccgcagttg ttggaaaaac tcaacgcctg
    51901 gtttgccaac tacgatcctg atgtgatcat cggttggaac gtggtgcagt tcgatctgcg
    51961 aatgctgcaa aaacatgccg agcgttaccg tcttccgctg cgtcttgggc gcgataatag
    52021 cgagctggag tggcgcgagc acggctttaa aaacggcgtc ttttttgccc aggctaaagg
    52081 tcggctaatt atcgacggta tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt
    52141 ctcgctggaa actgtcgctc aggagctatt aggcgaagga aaatctatcg ataacccgtg
    52201 ggatcgaatg gacgaaattg accgccgttt cgccgaagat aaacctgcgc tggcaactta
    52261 taacctgaaa aattgcgagc tggtgacgca gatcttccac aaaactgaaa tcatgccatt
    52321 tttactcgaa cgggcaacgg tgaacggcct gccggtggac cgacacggcg gttcggtggc
    52381 ggcatttggt catctctatt ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct
    52441 cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg atggattcac ggccagggct
    52501 ttatgattca gtgctggtgc tggactataa aagcctgtac ccgtcgatca tccgcacctt
    52561 tctgattgat cccgtcgggc tggtggaagg catggcgcag cctgatccag agcacagtac
    52621 cgaaggtttt ctcgatgcct ggttctcgcg agaaaaacat tgcctgccgg agattgtgac
    52681 taacatctgg cacgggcgcg atgaagccaa acgccaaggt aacaaaccgc tgtcgcaggc
    52741 gctgaaaatc atcatgaatg ccttttatgg cgtgctcggc accaccgcct gccgcttctt
    52801 cgatccgcgg ctggcatcgt cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa
    52861 agcgttgatt gaagcacagg gctacgacgt tatctacggc gataccgact caacgtttgt
    52921 ctggctgaaa ggcgcacatt cggaagaaga agcggcgaaa atcggtcgtg cactggtgca
    52981 gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa cggctgacca gcgcattaga
    53041 actggagtat gaaacccatt tctgccgttt tctgatgcca accattcgcg gagccgatac
    53101 cggcagtaaa aagcgctacg ccgggctgat tcaggagggc gataaacagc ggatggtgtt
    53161 taaagggctg gaaaccgtgc gcaccgactg gacgccgctg gcccagcagt ttcagcagga
    53221 gctatacctg cgcatcttcc gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga
    53281 caaactgatg gcgggtgaac tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc
    53341 gctgagcgag tatcagcgta atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga
    53401 agaaaaccaa aagcgtggtc gccccttgca atatcagaat cgcggcacca ttaagtacgt
    53461 atggaccacc aacggcccgg agccgctgga ctaccaacgt tcaccactgg attacgaaca
    53521 ctatctgacc cgccagctac aacccgtggc ggagggaata ctccctttta ttgaggataa
    53581 ttttgctaca cttatgaccg ggcaacttgg gctattttga gcaaaaaaag agttcgccag
    53641 ataccatttt gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc
    53701 ctggacctga ataacaccac tacctcataa gcacggtagc aggtggttat tgcctgcaat
    53761 taaagatata gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata
    53821 cagaaagcga attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac
    53881 ttttcccatc tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg
    53941 tgatgttcaa ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag
    54001 aagtaaaaga agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt
    54061 ccggcgtagc cctgcgtgaa gttttccttg atagcaaaat ggtgttcagc aaaccgcagg
    54121 accgcctgtt tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc
    54181 gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca
    54241 gcctgatccc gcaccagctc aacatcgctc atgatgttgg ccgccgccac gcgccgcgcg
    54301 tcctgctggc tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc
    54361 agcaactgct ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc
    54421 atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg
    54481 agcgttatgc cgaagctcag cacgatgctt acaacccgtt cgacaccgaa cagctggtga
    54541 tttgctcgct ggattttgcc cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg
    54601 aatgggacct gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa
    54661 gccgtgaata tcaggccatt gaacaactgg cagagcacgt gccgggcgtt ctgctgctga
    54721 ccgcgacccc ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc
    54781 cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg
    54841 cggacgccgt tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc
    54901 tcggcgagat gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca
    54961 gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca
    55021 ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc
    55081 tgcacaccat taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca
    55141 ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta
    55201 tttatcagga atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt
    55261 ggctgatggg ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag
    55321 ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg
    55381 tgttccacga aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag
    55441 aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc
    55501 agttcgccag ccacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc
    55561 gtattggtcg tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc
    55621 tggagaaaac cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg
    55681 agcacacctg cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact
    55741 atctggcttc accggatcaa accgaaggct ttgacgatct gatcaaaaac tgccgcgagc
    55801 aacatgaagc gctgaaagca cagctggaac agggtcgtga ccggctgctg gaaatccact
    55861 ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata
    55921 ccaacctgat cgccttcgcc atgaacctgc tcgatattat cggtatcaat caggacgatc
    55981 gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg
    56041 gcctgtcgga agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag
    56101 atgcacagtt tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt
    56161 ctggcgatac cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta
    56221 cgctgttggt ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca
    56281 accgcttcct gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg
    56341 cggcgcaggt agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg
    56401 gcagcaaact ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag
    56461 cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa
    56521 aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg
    56581 acgacgaact gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg
    56641 caggttggcg tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa
    56701 aatggggatg gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga
    56761 tgaccacatt atggtggtta acaagccgag cggtttgttg tctgtgccgg ggcgtctgga
    56821 agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt
    56881 gcatcgtctg gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga
    56941 gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt
    57001 ctgggggcat ccatcccctg cggaaggtct ggtggatctg ccgctgattt gcgactggcc
    57061 aaaccgcccg aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga
    57121 agtggtggag tatgcgccgg ataacacggc aagagtggtg ttaaaaccga ttactgggcg
    57181 ttcgcatcaa ttgcgtgtgc atatgctggc gcaggggcat ccgattctcg gcgatcgttt
    57241 ttatgcatca ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt
    57301 gacgattacc catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta
    57361 atagtcacgc tggcgcagtt cggtaggcgg ataaggcatt cacgccgaat ccggcaattg
    57421 tgcgccgatt gcctgatgcg atgcgggccg cgtcttatca ggcctacagt ttaaaactta
    57481 tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg
    57541 cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg
    57601 ttcactcatc agcttacggt aggctcgttt aatggtggtc gcatcatccg tcggcttcac
    57661 acccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc
    57721 accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa
    57781 aaactggtca aactgggcgc gggagatccc taattcttct gcaatgacat acagcaccgc
    57841 ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga tctccagaaa
    57901 catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg
    57961 cagcgggtaa ttgtctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc
    58021 gccatgaagg ttcattcggt ccatcagctg gctggcgata tgaatatcag cctccgtgac
    58081 gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc
    58141 ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat
    58201 atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac
    58261 ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat
    58321 cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgca gtcaattgtg
    58381 ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact
    58441 gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc
    58501 gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta
    58561 tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag
    58621 ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg
    58681 gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg
    58741 atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg
    58801 acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta cgaaccgttg
    58861 atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct
    58921 gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc
    58981 gccagggccg cggtaaagcg gacctgatga aacaacgtgg tgaaaaccgc tataccattc
    59041 tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta
    59101 gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc cgctttaagg
    59161 tgggtccagt accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc
    59221 gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc
    59281 tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc
    59341 atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg
    59401 gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg
    59461 atgacagttc acgtcgttgg ttgttctact ggcaacactc cggggtcatg gatcaagtgt
    59521 ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat gatttcgata
    59581 acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg
    59641 tgcaaaactt caatgccacc gtttcaacca agcagttcca ggtctttagc gagcagaaca
    59701 ccagtagcta ctcggcagag ccgcagttag acgttaacta ctaccagaat gatgttggtc
    59761 cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc
    59821 ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg
    59881 gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc aatcttgact
    59941 ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgtt atgccgcaat
    60001 tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata
    60061 cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca
    60121 tctataacta cgactcgtct ctactgcaat ctgactactc tggcctgttc cgggaccgga
    60181 cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt gtcacatctc
    60241 gcatatatga tgatgctgcc gttgaacatt ttaatatttc cgttggtcaa atctactatt
    60301 tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct
    60361 cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga ttgcgtggcg
    60421 ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc
    60481 gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg gaatatattc
    60541 aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg
    60601 taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg gcctactact
    60661 acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct
    60721 gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac
    60781 atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact
    60841 acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt
    60901 gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag
    60961 aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc
    61021 caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt
    61081 gatggattaa tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat
    61141 gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag
    61201 atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac
    61261 atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt
    61321 ctgaactaca acacctatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt
    61381 aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc cctggcgcag
    61441 caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg
    61501 ctgccggaaa acccgacttc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt
    61561 gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac
    61621 cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt gccgggtatc
    61681 ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc
    61741 gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg
    61801 accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag
    61861 gcccgtgtga aactggaaca gattgctgcc gatatcaaga gtggtaaaac gacttttgct
    61921 gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg tgatctcggc
    61981 tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa
    62041 ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat
    62101 acccgtaatg tcgataaaac cgacgctgcg cagaaggatc gtgcataccg catgctgatg
    62161 aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgcgc cagcgcctac
    62221 gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc
    62281 ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac
    62341 tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc
    62401 tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactgcg ggcacattaa
    62461 cgctacttcc tgtcgcgcta cgcgaatctg tcactgcggg gcagttagcg attgaaaatg
    62521 ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg
    62581 ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggt attccgttta
    62641 ctggtcatac cgagtttttc gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg
    62701 cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag
    62761 atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta
    62821 caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg
    62881 aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc
    62941 gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt cagccgaaat
    63001 atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa
    63061 aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat
    63121 cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt
    63181 ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt
    63241 ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt
    63301 gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg
    63361 ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat
    63421 cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact
    63481 gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg
    63541 tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca
    63601 tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt
    63661 gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca
    63721 atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc
    63781 caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc acccggttaa
    63841 agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat
    63901 tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc
    63961 ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc
    64021 ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag
    64081 gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct
    64141 tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg
    64201 ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc
    64261 cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc
    64321 ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc
    64381 atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg
    64441 acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa
    64501 gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc
    64561 ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg
    64621 ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg
    64681 aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg
    64741 cgtcgccagc ctttgctgca aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg
    64801 atcacgccgc agtgggatct acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg
    64861 ctatcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat gccaaataac
    64921 tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt
    64981 atgcgttttt gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagag
    65041 gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc
    65101 atcgcctttg gtcactgggc atcgctggag ggcaaaggta cgccggaagg tatatacgcg
    65161 ctggataccg gctgctgctg gggtggtaca ttaacctgcc tgcgctggga agataaacag
    65221 tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca
    65281 cagcctgata taggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta
    65341 aattctatac aaaattaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc
    65401 gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg
    65461 aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt
    65521 ggcaggaact gctcataaac acgaccgccg ccaatcacca tgatttctgg tacgtcacca
    65581 cacgccgcga tggcttcatc caccgacttc acccacgtta cgcgatcgtc cgtacccggt
    65641 tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaggta
    65701 tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca
    65761 ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca
    65821 atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata cgtaaagcgt
    65881 aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc tgctggcgag
    65941 tccacgctct ctccctggac tcgccgcatt acaatgaaac aaaaacaaac agttagctgt
    66001 aaagtgtgat ttacgtcact ctttattagg atgagggttt cgtttccggt tcatccgaca
    66061 tgttgccggt atgtttacct tcttcggttc cctgccagcc atgtcgttga attaatgaca
    66121 gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt ttatagacct
    66181 ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    66241 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    66301 gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc ttttcaacgc
    66361 ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata atctgcaaat
    66421 gcgggaagtg ttctttcacc atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg
    66481 cgttaatcag cacttccgct ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg
    66541 catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt
    66601 gatcgagcac caccattttc accccgctgg agagcagtaa acgtccggtg atctgcccaa
    66661 aacgaccgaa tccggcaatg attacccgcg gctgttcttc gtcgatctca tcggcttcac
    66721 gcgcctcttc agtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    66781 ccgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat
    66841 tcgccatctg cgccgcgcca aataccacaa aggcaaactc actgccctgc cctaacaaca
    66901 ccgcaaacca acgacgctgt ttatttggca cttgcaacgg tcgggcaatc agccacagca
    66961 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    67021 gcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    67081 ctttaaaggg ttcgatatcg ctctccagcg catgacggta ttccgagctt gccagcagta
    67141 cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa
    67201 agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta
    67261 caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgcac
    67321 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg
    67381 ccagtagcgg aatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg
    67441 caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    67501 caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc tgccagcgca
    67561 gcccaagtaa catgcagaac agccccagca ggccgccgca aatcaccatc tgcaatgcgc
    67621 caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt tcgaggccga
    67681 taataaacag catcagcacc accccaatct cggcaaagtg cagaatagat tcggcatcgg
    67741 tcaccagtcg cagcccccac gggccaataa tgcagccggc gatcaggtag ccaagtaccg
    67801 atcccagacc aagacgtacc gcaatgggta caatcagcgc tgccgaaccg agataaatca
    67861 gcgcctgaat cagcgtatgg ctatccatga tgggcctcct gccattccag cagacgttgc
    67921 ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg
    67981 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    68041 agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    68101 accgcccaca gcaaatgttt gccatgcagc gccttgccgc cgtgaccgta agcccagccg
    68161 tgcgacaaaa ctttatcgat ccaaagttta agaagcggag gaatgctgta ccactgcatc
    68221 ggatgctgcc agacgatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg
    68281 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctg
    68341 cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatta
    68401 taagaatcat aggtagcccg cgtgattacg cctttgtttt cccacaaagt gtagtcatat
    68461 attcacgcgg cttatagtca ataatattga ttcagaaaag agagaaatct gaacaattta
    68521 ccccattctc tgcccgcgga tcgtcatatt gctggtttgc gccagtgaca tccctcgatg
    68581 tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc gcccccatta
    68641 gcatcgtgtt actgataccg taattattga taaagatcgg cagtgcccag gtcgaaacaa
    68701 tggtgccaat acggcttaag gacataatca cgcccacggc agaggcgcgg atatctgtcg
    68761 ggaagagttc attaggatag agccactgca aattacccgg cccgccagag aaaaaggcat
    68821 acaccgcaaa ggccatcact accagccaga tccccatatc cgggatcagc cccaaaaccg
    68881 ccagcgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt
    68941 ttaaccacag catcggcgga atacagtcga gcataaagaa caggctaatc accacgttcc
    69001 ccagcgccgc gtttttgcca acacccaatc ccaacaaacc aacgatttgc gggccaaagg
    69061 tgtaaatggc gaacattggg atcacctggc aggtccagat ggcggcaaca aacagaacaa
    69121 aaggaaaatg gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc tgctcttcat
    69181 cgaaagccac cggttcgcca aacagtttta tcatcatctc ttcgcactct tttactcgcc
    69241 ctttgcgtaa taaccagcgg ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca
    69301 aacaggggat cgccgcgcta cccagcatcc agcgccagcc gccttccaca tcataaagcc
    69361 agtagccgac cagatcggcg caggtcgcgc cgacatacca catggcggcg ataaagctga
    69421 tggaaaatgc ccgctgacgg gtactggaga actcggtgat cattgaggtg gcgatgggat
    69481 aatctgcacc gatgacaatg ccgataagta cccgcatcac caacagttcg acgggggatg
    69541 aaacaaacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg aggaacattt
    69601 tgcgccgtcc gactttatcg gaaatatagc caaacagcga tgtgccaacg aacagcccgg
    69661 cgagcgttcc cgcgcccaac aagccaatcc agtcagcgtc cagttttagc gccggagtca
    69721 gttgctccag cgccacgcca atcattacca gtacataacc atcca
//
LOCUS       DAJTMK010000002        61288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTMK010000002 DAJTMK010000000
VERSION     DAJTMK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61288
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.002"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1012)
                     /locus_tag="OO135_000064"
     CDS             complement(<1..1012)
                     /locus_tag="OO135_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396056.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCD"
     gene            1113..1400
                     /locus_tag="OO135_000065"
     CDS             1113..1400
                     /locus_tag="OO135_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5396057.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            1372..2901
                     /locus_tag="OO135_000066"
     CDS             1372..2901
                     /locus_tag="OO135_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5396058.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(3071..4288)
                     /locus_tag="OO135_000067"
     CDS             complement(3071..4288)
                     /locus_tag="OO135_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5396059.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(4806..7082)
                     /gene="clpA"
                     /locus_tag="OO135_000068"
     CDS             complement(4806..7082)
                     /gene="clpA"
                     /locus_tag="OO135_000068"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5396060.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7113..7433)
                     /gene="clpS"
                     /locus_tag="OO135_000069"
     CDS             complement(7113..7433)
                     /gene="clpS"
                     /locus_tag="OO135_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5396061.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(8070..8351)
                     /locus_tag="OO135_000070"
     CDS             complement(8070..8351)
                     /locus_tag="OO135_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396062.1"
                     /translation="MTESEILRLIRCAGGISQLADEQAAQPDTVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKKPEWLRR"
     gene            complement(8354..8839)
                     /locus_tag="OO135_000071"
     CDS             complement(8354..8839)
                     /locus_tag="OO135_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCR5396063.1"
                     /translation="MDRELKNLMLNINQLAAIAGICRQTAAARLKNIQPAGGHDKLKL
                     YRVTDILTCFLDLPVPASLEEMEPHDRKAWYQSERERLRFEQETAQLIPADDVRKEMA
                     IWGEIVSEELAKLPNILARDAGLKPMAVNRVQSIIDDLRNQIISRMVKNDVVNEVAKQ
                     A"
     gene            complement(9097..11010)
                     /locus_tag="OO135_000072"
     CDS             complement(9097..11010)
                     /locus_tag="OO135_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014883662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCR5396064.1"
                     /translation="MNHWYTIKAADVRGAADISIYEEIGGFGVTAKQFAEDLKALGDV
                     SHINLRIHSPGGDVFEGIAIYNLLRNHPADITVYIDGVAASMASVVAMAGDRVVMPEN
                     AMMMIHKPWGISGGNAGDMRDYADLLDKVETVLIPAYARKTGKSAQEITAMLEDETWM
                     DGKECLKHGFADELLPSVRAMARIESKRTGDFLHMPETIKGMITPPQGAANIAGNEQK
                     RINGISEVFSLFGSRYDGIKMACLEDASCTPEMAREKLLNELGRESTPSNKNTPPHIY
                     AGNGNITGDAIRQGLYSRLGYERPERGNPYAMMSLFEMAQASLVDRGITVSGFINRSQ
                     VVNAAFTHSSSDFSHILAGGAEKSVLKGWQDSGETFQKWTRTGSLSNFHEAKRVGLNG
                     FSKLDKVPEGAEYKYITTSDKGVPIALATYGNIFSVTRQAIINDDLTQLTTIPMAMGR
                     AAARTVGNLVYLLLTSNGKFTDGKALFHADHKNLIAKDMDMEGLNEARKLMRLQEDAN
                     GDSLNITPAFVLVPAALESAAHRAILSSSSLFPVDGVGTINQNPGIINVVKDMAEVIV
                     EPRLDKANNKEWYVAAAKGMDTIEVAYLDGIDTPYLEEQEGFTVDGVAWKVRIDAGVA
                     ALDYRGLLKSSGA"
     gene            11103..11276
                     /locus_tag="OO135_000073"
     CDS             11103..11276
                     /locus_tag="OO135_000073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396065.1"
                     /translation="MMIYMVDPVLLKAHNGLRIQMNGTTPDIARVQVINTTHSNFLLL
                     TKLCTLLIDKSGR"
     gene            complement(11641..12063)
                     /locus_tag="OO135_000074"
     CDS             complement(11641..12063)
                     /locus_tag="OO135_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5396066.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQAEALAKHRKGELLSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALSRAKQQAGNDDPYGDNIPF"
     gene            complement(12060..12305)
                     /locus_tag="OO135_000075"
     CDS             complement(12060..12305)
                     /locus_tag="OO135_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396067.1"
                     /translation="MTAKHIKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            complement(12593..14410)
                     /locus_tag="OO135_000076"
     CDS             complement(12593..14410)
                     /locus_tag="OO135_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5396068.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGELTEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFRQHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDS
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMKAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWMPSCVSVTNDDSKN"
     gene            complement(14407..14706)
                     /locus_tag="OO135_000077"
     CDS             complement(14407..14706)
                     /locus_tag="OO135_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5396069.1"
                     /translation="MRTYLSGLTASGYAHPQIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFLSSLNVEIFICRFRREIPTSRNNHVSRVDVDKKLQELKNMIAAWREQK"
     gene            complement(14713..15033)
                     /locus_tag="OO135_000078"
     CDS             complement(14713..15033)
                     /locus_tag="OO135_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396070.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            complement(15026..16025)
                     /locus_tag="OO135_000079"
                     /pseudo
     CDS             complement(15026..16025)
                     /locus_tag="OO135_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323528.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(16078..16287)
                     /locus_tag="OO135_000080"
     CDS             complement(16078..16287)
                     /locus_tag="OO135_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5396071.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGSFPSRRNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAA"
     gene            complement(16707..17945)
                     /locus_tag="OO135_000081"
     CDS             complement(16707..17945)
                     /locus_tag="OO135_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5396072.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRHELNHTVQE
                     ALAPVTVKDALTYWLESYAKEKRTDHESLKSRINKHIISQIGAMPLDKCELRHWLACF
                     DQLANRNPVSAGFLLQVCKQALKYCRKRRYAVSNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIFPPYYSALIRLLIVFGCRTTELRRSEVREWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVAEYGRTAHRRINQTPWSL
                     HDIRHTFTTMLNDLGVDPHIVEQLTAHQMPGMQRVYNHSRYLDAKRDALNLWVDRLEL
                     LQNNDEKIVVMTPRIYPQNS"
     gene            18350..18574
                     /gene="cspD"
                     /locus_tag="OO135_000082"
     CDS             18350..18574
                     /gene="cspD"
                     /locus_tag="OO135_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5396073.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(18647..20593)
                     /gene="macB"
                     /locus_tag="OO135_000083"
     CDS             complement(18647..20593)
                     /gene="macB"
                     /locus_tag="OO135_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5396074.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKSITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(20590..21705)
                     /gene="macA"
                     /locus_tag="OO135_000084"
     CDS             complement(20590..21705)
                     /gene="macA"
                     /locus_tag="OO135_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5396075.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            21856..22812
                     /locus_tag="OO135_000085"
     CDS             21856..22812
                     /locus_tag="OO135_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5396076.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(22809..24467)
                     /locus_tag="OO135_000086"
     CDS             complement(22809..24467)
                     /locus_tag="OO135_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5396077.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNIPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            24893..25588
                     /gene="aqpZ"
                     /locus_tag="OO135_000087"
     CDS             24893..25588
                     /gene="aqpZ"
                     /locus_tag="OO135_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5396078.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVSHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            26083..26982
                     /gene="lysO"
                     /locus_tag="OO135_000088"
     CDS             26083..26982
                     /gene="lysO"
                     /locus_tag="OO135_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5396079.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQASLKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            27126..28778
                     /gene="hcp"
                     /locus_tag="OO135_000089"
                     /gene_synonym="priS"
     CDS             27126..28778
                     /gene="hcp"
                     /locus_tag="OO135_000089"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5396080.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            28790..29758
                     /gene="hcr"
                     /locus_tag="OO135_000090"
     CDS             28790..29758
                     /gene="hcr"
                     /locus_tag="OO135_000090"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5396081.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            29891..31609
                     /gene="poxB"
                     /locus_tag="OO135_000091"
     CDS             29891..31609
                     /gene="poxB"
                     /locus_tag="OO135_000091"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5396082.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            31646..32647
                     /gene="ltaE"
                     /locus_tag="OO135_000092"
     CDS             31646..32647
                     /gene="ltaE"
                     /locus_tag="OO135_000092"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5396083.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            32658..34088
                     /locus_tag="OO135_000093"
     CDS             32658..34088
                     /locus_tag="OO135_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5396084.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            34187..35200
                     /locus_tag="OO135_000094"
     CDS             34187..35200
                     /locus_tag="OO135_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5396085.1"
                     /translation="MKVLVTGTTSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(35197..36027)
                     /gene="amiD"
                     /locus_tag="OO135_000095"
     CDS             complement(35197..36027)
                     /gene="amiD"
                     /locus_tag="OO135_000095"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5396086.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(36024..36347)
                     /locus_tag="OO135_000096"
     CDS             complement(36024..36347)
                     /locus_tag="OO135_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5396087.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            36473..36988
                     /locus_tag="OO135_000097"
     CDS             36473..36988
                     /locus_tag="OO135_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5396088.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            37206..37934
                     /gene="artP"
                     /locus_tag="OO135_000098"
     CDS             37206..37934
                     /gene="artP"
                     /locus_tag="OO135_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5396089.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            37952..38683
                     /gene="artJ"
                     /locus_tag="OO135_000099"
     CDS             37952..38683
                     /gene="artJ"
                     /locus_tag="OO135_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5396090.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            38690..39406
                     /gene="artQ"
                     /locus_tag="OO135_000100"
     CDS             38690..39406
                     /gene="artQ"
                     /locus_tag="OO135_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5396091.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            39406..40074
                     /gene="artM"
                     /locus_tag="OO135_000101"
     CDS             39406..40074
                     /gene="artM"
                     /locus_tag="OO135_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5396092.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            40365..41096
                     /gene="artJ"
                     /locus_tag="OO135_000102"
     CDS             40365..41096
                     /gene="artJ"
                     /locus_tag="OO135_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5396093.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFANLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(41271..42398)
                     /gene="rlmC"
                     /locus_tag="OO135_000103"
     CDS             complement(41271..42398)
                     /gene="rlmC"
                     /locus_tag="OO135_000103"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5396094.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIACAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(42439..42927)
                     /locus_tag="OO135_000104"
     CDS             complement(42439..42927)
                     /locus_tag="OO135_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5396095.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(42987..43832)
                     /gene="potI"
                     /locus_tag="OO135_000105"
     CDS             complement(42987..43832)
                     /gene="potI"
                     /locus_tag="OO135_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5396096.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGAMPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(43829..44782)
                     /gene="potH"
                     /locus_tag="OO135_000106"
                     /pseudo
     CDS             complement(43829..44782)
                     /gene="potH"
                     /locus_tag="OO135_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
     gene            complement(44792..45925)
                     /gene="potG"
                     /locus_tag="OO135_000107"
     CDS             complement(44792..45925)
                     /gene="potG"
                     /locus_tag="OO135_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5396097.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(46020..47132)
                     /gene="potF"
                     /locus_tag="OO135_000108"
     CDS             complement(46020..47132)
                     /gene="potF"
                     /locus_tag="OO135_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5396098.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(47483..47959)
                     /locus_tag="OO135_000109"
     CDS             complement(47483..47959)
                     /locus_tag="OO135_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5396099.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(48047..48805)
                     /locus_tag="OO135_000110"
     CDS             complement(48047..48805)
                     /locus_tag="OO135_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HCR5396100.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLTRQAAESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEG
                     DFRSNLHRGGAASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPG
                     LEGIEKTTGIDIAGKMIRWIERYATTEYCLKTGG"
     gene            complement(48866..49588)
                     /gene="nfsA"
                     /locus_tag="OO135_000111"
     CDS             complement(48866..49588)
                     /gene="nfsA"
                     /locus_tag="OO135_000111"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5396101.1"
                     /translation="MTPTIELIGGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(49572..49859)
                     /locus_tag="OO135_000112"
     CDS             complement(49572..49859)
                     /locus_tag="OO135_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5396102.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            50019..50276
                     /gene="grxA"
                     /locus_tag="OO135_000113"
     CDS             50019..50276
                     /gene="grxA"
                     /locus_tag="OO135_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5396103.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLPQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(50306..50683)
                     /locus_tag="OO135_000114"
     CDS             complement(50306..50683)
                     /locus_tag="OO135_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5396104.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATYMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            50953..52638
                     /locus_tag="OO135_000115"
     CDS             50953..52638
                     /locus_tag="OO135_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5396105.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLAAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(52874..53092)
                     /locus_tag="OO135_000116"
     CDS             complement(52874..53092)
                     /locus_tag="OO135_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5396106.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(53183..54283)
                     /locus_tag="OO135_000117"
     CDS             complement(53183..54283)
                     /locus_tag="OO135_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR5396107.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            complement(54280..54765)
                     /locus_tag="OO135_000118"
     CDS             complement(54280..54765)
                     /locus_tag="OO135_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5396108.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     L"
     gene            complement(54762..57840)
                     /locus_tag="OO135_000119"
                     /pseudo
     CDS             complement(54762..57840)
                     /locus_tag="OO135_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            complement(57833..57952)
                     /locus_tag="OO135_000120"
     CDS             complement(57833..57952)
                     /locus_tag="OO135_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5396109.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            complement(57967..58269)
                     /locus_tag="OO135_000121"
     CDS             complement(57967..58269)
                     /locus_tag="OO135_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5396110.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            complement(58324..58839)
                     /locus_tag="OO135_000122"
     CDS             complement(58324..58839)
                     /locus_tag="OO135_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR5396111.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            complement(58849..60021)
                     /locus_tag="OO135_000123"
     CDS             complement(58849..60021)
                     /locus_tag="OO135_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR5396112.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQNPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            complement(60164..60730)
                     /locus_tag="OO135_000124"
     CDS             complement(60164..60730)
                     /locus_tag="OO135_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5396113.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGLRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            60758..>61288
                     /locus_tag="OO135_000125"
     CDS             60758..>61288
                     /locus_tag="OO135_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5396114.1"
                     /translation="MMPVSHYLCIFLNVGLGEGSALPVGVPVPWPSATPPTGWLKCNG
                     AAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDDGRGVDNGRGLLTLQDGAIVSHNHYW
                     GIWTSRTNDQTLESFTGTTILKQITPLSPAIDFDNYPIPNPAITEGGVVAATTKPAGA
                     NETRPRNVAFNYIVRAA"
BASE COUNT        14901 a        16415 c        14678 g        15294 t
ORIGIN      
        1 catcacaacc attcttatca ttaatattaa atacaagatc tttcagacgg gaaatatatt
       61 cctgagattt atcatcaagt gaaacactat aacgagacaa tgttccttct gcactgtttt
      121 gaaacttata aaacatttcc agtaattttt ttgcataagg cgttgtatca aagctggtaa
      181 agtcataaag atattgcgca gcgtaaccat acaaaccatg aatccagagt ccttctctca
      241 ccaacactct gagataatat gcaaggcttt gaagtatctt cgtctttcgt tgtatatcaa
      301 gtaagacact taatgaataa aatgcattat agaatttatc aatattcctg agcactttct
      361 ccagttcatc cttatccagc ttcttctccc ggtaatcctt tttatattca tttttgatag
      421 ccatgatata ttgttgcatc aggctcttgc ttttcccgtt atccttccgt atagttcctg
      481 tatatttccc cagagcatac agctttccat tttcatgaaa atcttccttg ttgaatccca
      541 gttcagccag atattcccga agataacgtt taacagcagc ttcatcgctt tctgtatcag
      601 cgtgatcaag acaaggacaa tacgatttct gtaaaaaatt ataaatatga taataaaaag
      661 cagagtcatt ctctggtctg tatgtattat acatatctac aacaagaaaa ataaccccaa
      721 aagtgcgcag acaatagctg cgaaggaatt caggttttag tgtggaatgc actggataag
      781 aattcatcat gtaataaaaa tgttcatatc gggcatatat gatccgccgt tgctcctgac
      841 tgaacacccc ttcagcatca ataatacgac tgatctcatt ataacgttga tcccagaatt
      901 tttgatgcat ttcaaaattg gtactcttcc gcttgtgtgc cggattctcg ccttccttaa
      961 acaaccccaa aacagtacaa atatcataca acatatttgt aaatgtgcgc atatgctgtg
     1021 ctctccggtt ggtttgcatt ttatcgcaga ggacattctg agcttaacgt atcaaactga
     1081 cacaacgaca tgcaatgcat atggagaaca atatgtcaaa tcaaaccgga cagctttaca
     1141 cgccttcacg catcctgcgt atggctgaac ttacccgact gctgggtata tcaagatcta
     1201 ctatttacgt gaaaataaat ccggcctcga aataccacga ccctaccttt cccaaaccaa
     1261 taaggcttgg tagtgcatcc gtaggctggc gggcatctgc catcgatgag tggctgatgt
     1321 tacatacggc accagcctgg tctgaacctg aaaacaaact aaaggaactt aatgatgacg
     1381 acaaaactat cactccctga acttgacaca actaaactgc ctgacaggtg ccaggcatta
     1441 cttgatgaaa tgcatgaaga aacaggaata cgccgtgaaa tacttctgtc tgttatgctg
     1501 acggtcatgg ctgcctcggt ccaggatacg catgaagttg aactttccgg agggcaacgt
     1561 accagccttc agatatttat gtgtctttca tcagcatcag gcagtggaaa aacctctgcc
     1621 tgcgcaaaat taatcgctcc tgttcatgaa acagaagaag aactacatca ggcatatatc
     1681 gacgataaaa aaaactatga ccgaatgatg gaaatgtgga caacagataa aaaaatcctg
     1741 gagcggagat ataaaaagga aattgaaaga tccccggaaa atgctgctgc tgcacgagca
     1801 gcacttgaaa agtgtatagc aaataaaccc gaacctcccg tacaacaggt tctcatcgtg
     1861 aatgacgcga caccagaagg gatagctttg aaactcagcc agtcaccctc tcttctgttg
     1921 ctgtctgatg aaggaggtac tattctggat aagcgtttcg aacgcaaatc ggcactgtac
     1981 aacacattat ggagtgggca accagtcaac gttgaacggg catccagacc cgggttccgg
     2041 ctgaatgatg tccgtctcac tatacttata ctgactcaac cggtaatatt taataagttt
     2101 tttactctca ctggcgatca gatccggggc aatggctttc tggcccggtt gctattttgc
     2161 gaacccggag caattaaaat aatgacgaca gaaccgcata ctgccactcc tgttgtgacg
     2221 caacaatgtg caagctgttt tcgtaagaaa agttttggca gtcagatcag agactctctg
     2281 agagcatcca gagaaagacg tgcaaaaagt aaagagcgca tctgcatgac attatcaaat
     2341 gcagcatccc gcgcactgga gcgctttcac aaagaaaata tggatactgt cagacagaat
     2401 cctcatatgg gtacatttga agacattatt gtcagaaaaa gagagcaggt tgtcaggata
     2461 gcagcacttc tggaactgga gaaagatcca gactcgacag taataacgct ggaaagtact
     2521 aatagcgcca tttatctcat tgaattttat ttcaagcatc ttatatataa acttgaatcc
     2581 cttcgggaaa tatccccggc tgagaaactt gataaatggc ttcagaaaag aattatcaca
     2641 acagcaggtt acatattcca gaaaagttat attttacaat atgccccata tgctctcagg
     2701 aaaaaatgtg ttctggatga agcacttgac atactggcgg aacaaaggaa aataaggatt
     2761 gatgataacc tggttgttta tataggaaac acgattacac catccgaact ggcaaaaaaa
     2821 ctaaatattc ctgcattcga tgccggagtt ttcatttgtg atcatcaaaa catactgaag
     2881 tatcaccgaa acagactata gatactatcg gacaaaaaat aaattatcac aagtgtatta
     2941 ttcgttataa taatgaataa tcgttatctg agtgcaatgc caggttttca ttacgaactg
     3001 gcattgcatt tatttttcaa aaaagaaagc agtaaccatt tggctactct gagtatgcca
     3061 gtccatgaaa ctaagcgaat tccttagtgt ttttagcaag ttgagacatc acataatccg
     3121 cccatgcctg catcatcggt acacgctgct ccagtagatc agtacgatga tatgccgcct
     3181 caaccttatt tttcagcgta tgagcgagcg ccctttccgc cagatcccgc gaatacccct
     3241 gttcactaca ccagtccctg aatgttgagc gaaaaccatg tgccgtggca actcgccccg
     3301 gaatgtcact gacggctttc tttttacgca gaaaacttgt caacaccata tcggaaagga
     3361 tctgctgctt tctgggtgaa gggaacacca gttcatcatg caggccacgt atattttcca
     3421 gaatgtaaat agcctgccgg gataaaggaa cacgatgctg tagcctagct ttcattcttt
     3481 ctgcaggtat agtccatacc cgcttatgaa aatcaatttc agcccagcgc attcccctgg
     3541 cttcgcccga gcgagttgct gtaagtatca ccattaataa cagtgcgcgg gtaacattat
     3601 aaggttcatc actatacaca ctggtcgcca caaaaagcgg taactgcctc cagggcattg
     3661 cgggttggtg ttcatcacgt cctcttgtct gctgaggaag caaatggtca accacatcaa
     3721 caggatttgc cacacaaaaa ccgtgcgccc atccccactg cataacaaca tgaatgcgct
     3781 gtttaacccg gcttgccgtt tctgacaagg ttaaccagac tggacgcagt gtttctgcca
     3841 cgtccgcagc cgtaatcgaa tccagcgttt ttgctcccag ttgaggaaac gcgtaattct
     3901 caagcgtcga taaccactgc cttacatgct ttggattctc ccatccagga gacagttctg
     3961 catgtacacg cctggctgca tcggcaaatg ttgggataac gactttctca gattcagcct
     4021 ttttaatctc cagaggatca tcacctgcag caagttgctc tcgcattatc cgggcagtac
     4081 gtgcagcttc agcaatactg acctctgggt aagttcccaa tccagcatta cgtctttttt
     4141 gtgtcaccgg acttacataa cgaaaaaccc atttcccccg cccctttact gaagaaggat
     4201 gaagggtcag tccggtaatt cccccatggg gcaatggttt gtcatcaggt ttgatatgtc
     4261 ttgctttcgt atccgtcaat actgccatac gctaattccc gtcactctgg tatgccatcc
     4321 agtatgccat tagtccctcg cttcactaag aatacattag ataacatcgg acaacaaaaa
     4381 gtgtaataca ttgtttataa agaaattata taacccccct ggattaagtc agatttattt
     4441 caggcggtcc ccctcaccgc catctttaaa gaagagctcg tacgaaagtg cgagcttttt
     4501 tttcgcattt tatctgtctg tacggcagtg aacatggtgg gcgggtggag tatgttacct
     4561 gtgaatttct aagctgcctg tacggcagtg aactcaaata ctacacgttt agcccgttgt
     4621 taataaagca aaaccatccc attctcctgc agcacccttt tttatcctta ccttcccgca
     4681 gcactaatta tcaacaactt accatcatca ttaaaaaaag ggtctgaacc tcctccaccc
     4741 cataaacaaa aagtccgtaa cctctttcga gattacggac ttgaccaacc tacctaacaa
     4801 tcagattaat gcgctgcttc cgccttgtgc ttttgtgcac tctggaatcc gtaagtcagc
     4861 tcatttttct ctttatccag cgcgacggtg acctgaccgc cgtccaccag cgaaccaaac
     4921 agcagttcgt tggcgagcgg ttttttcagg ttgtcctgga tgacacgcgc catcggacgt
     4981 gcgcccattg cccggtcgta acctttctcg gccagccagt tacgcgcttc ctggctcact
     5041 tccagagaaa cacctttctg atccagctga acctgcaact cgacgatgaa tttatccacc
     5101 acctgatgga tcacgtcagt tgacagatga tcgaaccaga taatgttgtc gagacggtta
     5161 cggaattccg gtgtaaagat cttcttgatc tcttccatcg catcggtgct gttatcctgg
     5221 tggataagac caatggattt acgctcagtt tcacgtaccc cggcgttggt ggtcatcacc
     5281 agcaccacgt tacggaagtc cgctttgcgt ccgttgttat cggtcagcgt accgttgtcc
     5341 atcacctgca acagaatatt gaacacgtcc ggatgcgctt tctcgatttc gtccagcaac
     5401 agcaccgcat gtggatgctt gatgaccgca tcagtcagca aacctccctg atcaaaacca
     5461 acgtatcccg gaggcgcacc aatcagacgg ctgacggtat ggcgttccat atactcggac
     5521 atatcaaagc gcagcagctc aatgcccaac gctttcgaaa gctgtaccgt cacctctgtt
     5581 ttcccgaccc cggtagggcc ggcaaacagg aacgaaccaa ccggtttatg ttcgtgacct
     5641 aaacctgcac gcgccatctt aatggcttca gtcagcgcct caatggcttt atcctgaccg
     5701 aagaccagca ttttcaggcg atcgccgagg tttttcaggg tatcgcggtc actctgagaa
     5761 acactcttct ctggaatgcg cgcaatacgg gccaccacgg actcaatatc cgccacatta
     5821 acggttttct tgcgtttgct taccggcatc aggcgtgcgc gagcgcccgc ttcgtcgata
     5881 acgtcaatgg ctttatccgg cagatgacga tcgttaatgt atttcaccgc cagctctacc
     5941 gctgcacgca ccgcttttgc ggtataacgc acgtcgtggt gcgcttcata cttcggtttc
     6001 aggccattga tgatttgaac agtttcttcg atcgacggtt cagtaatatc aattttctgg
     6061 aagcgacgcg ccagagcacg gtctttctcg aaaatgttgc tgaactcctg ataggttgtc
     6121 gaaccaatta cgcgaatttt accgctggag agcaacggtt tgatcaggtt agccgcatcg
     6181 acctggccac cagacgctgc acccgcaccg ataatggtgt ggatctcatc aataaacagg
     6241 atgctgttag tgtcctgctc cagctgcttg agcaacgctt taaaacgttt ttcaaagtcg
     6301 ccgcgatatt tagtgcccgc taacagagaa ccgatatcga gagagtaaat cgtacagtca
     6361 gccatcactt ccggcacatc gccctgaaca attcgccagg caagaccttc cgcaatcgcg
     6421 gttttaccga caccagattc ccccaccagc agcgggttgt ttttacggcg acggcagaga
     6481 acctgaatag cacgctccag ctccttctca cgaccaatca gtgggtcgat tccgcccacg
     6541 cgcgcaagct gattcaggtt cgtcgtgaaa ttctccatac gttcctcccc accagcttgt
     6601 tcttcgctgt ttggctggct gccaggatca gaagactgtg tcggctcgtc tttacgcgtg
     6661 ccatgagaga taaagttcac cacatcgaga cggctgactt catgtttacg caacagatat
     6721 gccgcctgcg actcctgttc gctaaagata gcgaccagaa cgtttgcacc ggttacctca
     6781 ttgcgaccgg aggactggac atggaagacc gcacgttgca gtacacgctg aaaactcagc
     6841 gtcggctgtg tgtcgcgctc ctcttcactg gcaggcagaa cgggtgtggt ttgttcaata
     6901 aaggcttcca gttcctgacg gagcgcaacc aaatccacag aacacgcttc cagcgcctcc
     6961 cgggcagatg ggttactgag cagcgccagt aacaagtgct cgacggtcat aaactcatga
     7021 cggtgctcgc gcgctctggc gaaagccata tttaaactga gttccagttc ttgattgagc
     7081 ataggcacct cccccaattt ttatgcctgc attcaggctt tttctagcgt acacagcaat
     7141 ggatgctcat tctccctcgc gtacttgttc accatcgcca ctttggtttc tgcaacctcg
     7201 gcggtaaaga ctccgcaaat ggccttcccc tggtagtgaa cagcgagcat caattgcgtt
     7261 gcacgttcta catcataaga aaagaatttt tgtaacacgt caataacaaa ctccatcgga
     7321 gtgtaatcat cattgactaa tatcacttta tacatagatg gcggttttag cgcgtcgcga
     7381 actttttctt ccgccagttg atcaaagtcc agccagtcgt tcgttttacc cattgtcagt
     7441 tatcatcttc ggttacggtt atcggcagaa cgccctgccg ctgacaagag cttatttgcc
     7501 tgaccaatct actccacatg atagataact atcatttata gcatttggct tattagcctt
     7561 tatttttcat tgagttgcaa acaataagct cgtcaaaaac agtaaaatta actacaccat
     7621 taactacacc gttccacgaa caatatcgaa caaccacgaa caaagatcaa cacgctttaa
     7681 tcgttatcat tttccggcta tcagtcatga aaccagttat cggcatcagg cccaccagcg
     7741 tgaagcgttt ttcatttttc gcgggtataa aaaatcgatg gggctggcag tccggaagac
     7801 gtcaggtcac agggatttga cccgcccctc cctgtgcatg agcacaataa gtaaccgaag
     7861 gcggaattcc gccctcggta ctcacatatg agaaacgata tttcttccac agataccctt
     7921 tatttgggta tcgcgagtaa gccccaaaat ctgggtgtta ctcttactac ccaaactacg
     7981 ggtagtttcc gtaatttcat ggtcgagttg cagatctgca actcacccac cagccaacgc
     8041 acttttgcgc tatcaggaat atcaacaaat taccgccgca accattccgg cttcttccac
     8101 tgataagtat ttttcgcgct ctccctccgt tgttgagaac ggcgacggta tgccagcaac
     8161 tcaaggactc tggttcgtat gttgcgcata tccacgccgt taagctcaat accgtcacgg
     8221 cgcatcacct cagccaccac acgcgcgtaa ttttcagcgg tgactgtatc cggctgcgcg
     8281 gcctgttcgt cagccaactg gctaattcca ccagcacagc ggattaatcg cagtatttca
     8341 gattcggtca ttatcatgcc tgttttgcga cctcattcac tacgtcattt tttaccatcc
     8401 ggctgataat ctgattacgc aaatcgtcaa taattgactg cactctgttt actgccatcg
     8461 gtttaagccc ggcatcacgt gccagaatat tggggagttt tgccagttcc tcgcttacga
     8521 tttcccccca tatagccatc tcttttcgca catcatcggc gggtatgagt tgcgccgttt
     8581 cctgttcgaa cctgaggcgc tcacgttcgg actgatacca ggccttacgg tcatgtggct
     8641 ccatttcttc cagtgatgcc ggaacgggaa gatcaagaaa acaggtcaga atgtcagtca
     8701 cccgatagag ttttagcttg tcatgccctc cggctggctg gatgtttttc agccttgccg
     8761 ccgcagtctg acgacatatt cccgctatcg ccgccagttg attaatgttc agcatcagat
     8821 ttttcagttc tcgatccata cccgctccag aatgttttaa acatgcatct tgcgaacaac
     8881 tttcagaaga ggtcaaaaaa tgcgctacat gttgagcata aaacaagcaa aattaacatg
     8941 ccaaaaataa aaatacttaa aattcaatac aatagaaaga tgatgatgac gaatgaaaat
     9001 gcaaaaacta gcctttttcc gcgccgctcc cgccccgtgg cagaccaccc caccgggagg
     9061 acccgtaaaa aaaggcggct atcgccgccc ttgttgtcat gctccactcg atttcagtaa
     9121 tccgcgatag tcgagggccg cgacacctgc atctatgcgc accttccagg cgacgccatc
     9181 aacagtaaag ccctcctgct cctcaagata tggcgtatca ataccatcaa gataagcgac
     9241 ctctatcgtg tccataccct tcgccgctgc cacataccac tccttgttat tggctttatc
     9301 aagacgtggc tcaacgatta cctccgccat atctttcacc acgttaatga tgccggggtt
     9361 ctgattgata gtgcccacgc catcaaccgg aaagagtgat gacgatgaca gaatggcgcg
     9421 atgtgcggca gactccagcg cggcggggac caggacaaat gcgggggtaa tattcagcga
     9481 atcgccgtta gcgtcttcct ggaggcgcat cagcttacgg gcttcgttaa gcccctccat
     9541 gtccatatcc ttcgcaataa gatttttatg atcggcatgg aataacgctt taccatccgt
     9601 aaacttgccg ttgctggtta acaggagata aaccagattg ccaactgttc tggcagctgc
     9661 gcgtcccatc gccatgggga ttgtagttaa ctgggtcagg tcatcgttga taatggcctg
     9721 acgggtaacg gaaaaaatat tcccgtacgt ggccagcgca ataggtacac ctttatcgct
     9781 ggtggtgatg tatttatatt ccgcgccttc cggtacttta tccagctttg aaaaaccatt
     9841 cagaccaacg cgctttgctt catgaaagtt tgaaagcgaa ccggtacgcg tccatttctg
     9901 gaacgtttcg ccgctgtcct gccagccttt cagtactgat ttttcagcgc caccagccag
     9961 aatatgagaa aaatcgctgc tgctgtgtgt aaaagccgca ttaacaacct gcgagcgatt
    10021 aataaaaccg ctcacagtga taccacgatc aaccagtgat gcctgggcca tttcaaaaag
    10081 gctcatcatc gcgtaagggt tgcctcgttc agggcgttca tacccaagac gggaataaag
    10141 cccctgacga attgcatcac ctgttatgtt tccgtttccg gcatagatat gaggcggggt
    10201 atttttattg gatggcgtgg actcgcgccc cagctcgttc aacagctttt cacgggccat
    10261 ttccggtgta catgatgcat cttccagaca cgccattttg atcccgtcgt aacgactgcc
    10321 gaacaagcta aacacttcac ttattccatt gatgcgcttc tgttcattac cagcaatatt
    10381 tgccgctccc tgtggcggtg taatcattcc tttaatggtt tccggcatat gtaaaaaatc
    10441 tcctgtgcgt ttcgattcaa ttcgcgccat tgctctgacg gatggcaaca attcatcagc
    10501 aaaaccgtgc ttaaggcatt ctttcccatc catccaggtt tcatcctcca gcatggcggt
    10561 aatttcctgt gctgatttgc ccgtttttct ggcataagca gggattaaca cggtttccac
    10621 cttatccagc aaatcagcat aatcacgcat atcgccagca tttccgccag agatacccca
    10681 cggtttatgg atcatcatca tggcgttctc cggcataaca acacgatcgc cagccattgc
    10741 gacaaccgaa gccattgaag ccgcaacacc atcgatataa accgtaatgt ctgccggatg
    10801 attccgtagc aggttataga tggcgatgcc ttcaaacaca tcaccacctg gtgaatggat
    10861 cctcagattg atatgtgaaa catcgccaag ggctttcagg tcttccgcga actgctttgc
    10921 agtaacaccg aagccgccaa tctcctcata gatagatata tccgccgctc cgcgaacatc
    10981 cgccgcctta atggtgtacc agtgattcat tgctcactct ccgatgcaaa accactctga
    11041 ttaagccagg cgtttacggc atgcctgaca atctgcgcca cacctggtaa tggtttgtca
    11101 ggatgatgat ttatatggtc gatcctgtac tgcttaaggc gcataacggt ctgcgcatcc
    11161 agatgaacgg aaccacccct gatatcgccc gtgttcaggt cattaataca actcacagta
    11221 acttcctctt actgactaaa ctgtgcacat tattgatcga taaaagtggt agataaacat
    11281 atttctatca taaaaataga ttaatgagat tcagacacaa aaaagccgga taaaatccgg
    11341 cataaatatc ccgccatctg aacacgtttt gacacaggca actccacctg gcaggtgaaa
    11401 aacagattta tttatatatt tcaattaatt gcaaactggt ctaatgacag ggagaaaaaa
    11461 atattgtaca ggtgaaaaca gaaataactt ttaattatca ataaattact acacatgctg
    11521 ccgccgccat gaaaatgcaa aaaccagcct ttttccgcga cgctcccgcc ccgtggcagg
    11581 tcactacacc gggaggaccc ataaaaaagc cggattgctc cggcttctgt tactcgttgc
    11641 ttaaaacggt atgttatccc cgtacggatc atcatttccc gcctgttgtt ttgctctgct
    11701 cagtgcatca gtagcctgcc cctgttggcc ttttttgccg cccggtcgcg ccgttctcgc
    11761 actgattacg ctgtctgcga taacctgcca gccctgccgc gtctccccgt tctgtccggt
    11821 ccactggctc acctgcatgt tacccgccac gctcaggagt tcacccttgc ggtgctttgc
    11881 cagtgcttcg gcctgtctgc caaacgccag gacggataac cacatcgtcg ccgttccgtc
    11941 atctgcctgg ctgcacggaa gtgggaccgc catactcgcc atcgtcattt gtgtaccctt
    12001 gctggtggtc tttaactgcg ggtcagccac cagccgcccg taagccgcta tctgtgctgt
    12061 catgctgtct gctctccggt tttaacgttg atggttgtca cctgttccgc ttcggcaatc
    12121 tcccgttctg tcagcgtggc aaagtttgca gctgccgttg tcatgaatgc gctaatcagt
    12181 tcgggatgtg ctttcgcata tccttccccg gcgttgcggt cgatgatttt tatcgacacc
    12241 cttaaccagt gttccgtcaa atccagggcg tgcgattgtg atttttttat gtgcttcgct
    12301 gtcataggct ttatctcaca gcagtaaatt aaaatttttg cgttttaacc cttcacctgt
    12361 tcaccttttg atattttatc ttttaattca taatgttaag gggtgaacag tttcacaaaa
    12421 actattcacc aactgttcac cactgttcac ccttgaagct caataaacaa tcaaaaaggt
    12481 gaacagtgaa tagtttggtg aacagttcat aaataactgt tcaccctata atatactgat
    12541 ataaaagata tttataacag ggtgaacagt ggtgaacagt tattccataa gtttaatttt
    12601 tgctatcgtc attagtgacc gatacacatg atggcatcca gtcttctgat tcctccgtca
    12661 gtgtcacgtt tgaacgcaaa ccgtgcttcg ttttccgttt catatactcc ctgccatatt
    12721 ccgccattgc ccccggcata tctttaccga agcgcgtcag tgttacaggt ttaccaaacc
    12781 catgtgcctt catataagcc agataggcat gatagagata cctgcgcggg ctgaatggca
    12841 caatttcagc attacccact aacaggccat cacacattac cgacgccatg agatagccgc
    12901 agaagtccac cagcgaatca ccttcacgct ttatcgcgag tgcttcttct gatttctgct
    12961 gctcatataa aaggcgtctg gcttcgtcct ggtcagcaaa ccgtgtaagc agatgacgaa
    13021 tcactaccgc cagctcacct tctatttttt ccgccagcat tgaatcgcgt tcgttctccg
    13081 gtacaacttc cgaaaaattg aatatcaccc gacggcgcga gatccccccg ctgcggtcac
    13141 tgaatgacat agcgttattg ttaaccgcca gcactactgc cgggatacgc gttgaatatg
    13201 gggctttatg ttttgggtcg attgccacct tgtcaccgcc tgtaatagcc ttaattcccg
    13261 ccccatcacc agcgtagcgg gtcatatccg gcatgataat cagcgaaaag ccaaccacta
    13321 acgcgcgttc ccttgcatct tccagcgcct tcatgctcgc tgatactgta ttagccttac
    13381 ccgccagcat ggtgcaaatc tccgccatca cgcttttacc acttccccct ggacctgtta
    13441 cctcaatgaa taactgccag tcgtaccggt tcgccagcac catgaataat gcagccagta
    13501 cgcgatccgc cttgcggtca ttctcagcca ccgaacggcg taaccacttc cagaaattcg
    13561 gcgcatgtgt tgccagcgtt tccccctctg ctggtgggct gaaaggtaat tcactggcaa
    13621 ttaacaacca gtcgtttttg ttatgctgcc gaaaattacc tgttctggta tcaaataccc
    13681 cgttactgaa tccaatcagg ttacgggctg tattccccat tacaggcaaa cttaacttca
    13741 tggtatcgac cgccgattta atggcgttct gcgaatagct gatctccgca tcaatgaaaa
    13801 tctgcgccat agctcgctgt aattctttat cctgtactgg ctcccataca acgccgttgt
    13861 aatggtgaac agtgtcagag tcggcatgaa tcgccagttc accgccataa tgtgccagga
    13921 gaacttcgcc gcgttgactt gctcccatct ggttaagcgc cagtgatgaa gcgttatcgt
    13981 cttttacccg ctcttttttc tttacaggca gttcaactac ctttttcttt tccgtcagct
    14041 ctccccgctc acgttccaga tattcgcgcc agttctcccg tttctggctg tgcattcctt
    14101 cagggtaata atcagcatcc ctgacacctg ccgctgccag tttctgcccg atggtattaa
    14161 caagccccgg acgcaataac cccgcctggt agagacgcac gcgatagcgt ccgtccggta
    14221 cgatttgcag gttgtccagt tcggcaagtt gttgctctcc aagccagaca ggtggcacgt
    14281 tatcgccagc cagtcgcccg tcctgttcct gccactgctt cgcatgtgcc cacgcatcac
    14341 tacccgcaaa aatgatgact tccgtcattt tgtcacgcgg ctggtgtttt aaatttggcg
    14401 cttttttcat ttctgctctc tccacgcggc aatcatgttt ttcagttcct gtagtttttt
    14461 atccacatcc acacgtgaca catggttatt tctggaagtc gggatttccc gccggaatct
    14521 gcaaataaag atctccacgt tcagcgaact aagaaatgaa tagccatcac ggataaaata
    14581 cacacggtca aacatcagtt cttttaccgt tactctgtta ccgttcttat ccagataaat
    14641 agcgccgggg ataatttggg ggtgtgcata accgctggca gtcaagccag ataaatacgt
    14701 tctcatgatt atttatcccc gatttgaatc agtattcgct ttctttatag catttaatgc
    14761 atctgtggca ttttcaatgg tgcaccgtaa cgaaatatca aaatgtccaa gcattgccag
    14821 taacaaaccg atattaccca tatcaatgcg catggccttt tcgtcatagt cctcattttc
    14881 tgacgcatgc cacatcaggc taccaattga cgcaacagcc attgatatat tgtcagtagc
    14941 cccatccgca gcggaataaa cctttttagc aatatcatgc tcacagttaa aatgcggatt
    15001 aatcaggtac tggtaatttg acatatcagg catggtaccc cccctgacga atacgggcgg
    15061 cgaataccat cacgcagcca gccggggatt gctggcgtgc ttcctgttcg ctgctggcct
    15121 caatggtaat cacgcgcggt tgtgccgtgc tcagggcgat aaaacgccag atgtatttat
    15181 tcaggttgta cgagtcccgc ccttgcgggt gtatggtatg atttctcata gctgcctcga
    15241 tactctcgtt atcgttggtg gttagacgcc ccgtatgtgt ttccggcact gcggggcgtt
    15301 gctctttgta tttcaacaat cctttcggtg tgtttcatgt tatgagcgca tgaaacacac
    15361 gtcaaggctt tttgtatttc tttttttgtg tatactgaaa cacaccgatg attaggagtt
    15421 tcagaaatgg caacggctaa caaaaacgca aaatcacaac tgacaactgt cagagtccca
    15481 ctagatgtta tgcaagggat ggaatccgtt aagctggacg gcgaaagcaa tgccggattt
    15541 atcgtaaccg ccatgcgcgg agaaatagcc cgccgccaag cagaaggcag cggagaaaat
    15601 ccccttgtgt cgtcactgga tgccctggct aaggtcgaac aaatcggcat caaagcagcg
    15661 gaagaaatcg ggcagcttgt caccgttgca cgcgaagaac tccagcgtcg caagaccaaa
    15721 gaaccagagt aatcaccatc agcgccgtgg tgtaaggtat tacggcgcat tgctatgcag
    15781 gacaacacaa tgaccgataa agaattgacc aaaacattat caccggcacg gaaaagacgg
    15841 cgcagaaaga tagagcatga atcagaaaga ttcgcgccat gtgcttttgc tcttgagaaa
    15901 ttccttaaag agcacaggaa aaagctctcg ttgcaaacct tggaacgaac caaatctgac
    15961 tgatcacatt gcccaccagc cgcaaatgtg gcattgttgg tgatgcgttc aagtttagtg
    16021 tgtatccatt ggcgacctcc cccggtcgtc tttgttttat atgtcatatg ctccccttta
    16081 cgctgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa cctcggacag
    16141 cagccaacct acagaacgcc caccaagatt acggcgagac ggaaagctcc cttttttctc
    16201 cagttcgtag cgtgtagtgc gacacactcc agttaactta cgacattcat cctcacggat
    16261 tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac gtatataaac
    16321 ttttctctta gctgtagata tatgagatcg gatattactt agattctttt tcacctctta
    16381 aattaaaaac acaaccatac taaaggcttt gttcgctaag gttcgtagaa gctcgttagt
    16441 gtttaaaatc gtgtcacgag tttttaagcg acgcaacaaa aaatgtcgtc atttggcatg
    16501 tcacctgaat tacctcatta aaaacaaata gataaaccat caggtattgg aacaatcaag
    16561 aaacagagaa taagaactca gaagaagaca tttcaatttt tttcgatagt tgacacatat
    16621 aaagagaaga tatttgcaga tattccccga tattctaaga tctattcacg gctgtaatct
    16681 cttaaagaaa aaacagccgt aatttgtcaa gaattttggg ggtaaattcg cggggtcata
    16741 acaacgattt tttcatcatt gttctggaga agctcgagac ggtctaccca tagattaaga
    16801 gcgtcacgct ttgcatcgag ataacgggag tggttataga cgcgttgcat tcctggcatc
    16861 tggtgggcag taagctgttc gacaatatgc ggatccacgc ctaaatcgtt cagcatggtt
    16921 gtaaatgtgt gccggatgtc gtgcaacgac cagggggttt gattaatgcg tctgtgtgcc
    16981 gttcttccgt actctgctac ggatgattgc cctttcagtt ctccaagcaa taagcccgtg
    17041 tgcctgttct gctccaccag cttcatgacg aacggcaaga tcgcttccgg tattggcctg
    17101 aatattgcta ccttcgtttt gctgtgttct ttcggcacag tccagagcat ttctttaaaa
    17161 tcccattccc gaacctcgga gcgtcttagc tcagtggtac ggcatccgaa gacaatcaga
    17221 aggcgaatta aagcgctgta gtacggcggg aaaatttttt catccagtgc gcggagtagc
    17281 tcgccaagct ccttgtttgt tagcactcgc tcgcttattt ctgctttttt tcccacatca
    17341 cctacgacca tatcatcaag aacgttactg acggcgtagc gacgtttacg gcagtactta
    17401 agcgcctgtt tgcatacctg caacaaaaag ccggctgata ccggattacg gttagcaagc
    17461 tggtcaaaac aggccagcca gtggcgtagc tcgcatttat ccagcggcat agcaccaatc
    17521 tggctgatta tgtgcttatt gatacggctt ttcagtgatt cgtggtcagt gcgcttttcc
    17581 ttcgcatacg actcaagcca gtaagtgagc gcgtccttaa ccgtcacagg ggctaacgct
    17641 tcctgcactg tgtgattaag ctcatggcgt ggatttttcc cctccgccag ccaggcgcga
    17701 cactgtgccg ctttttccct ggctgctttc aggctcagat cgggatagtt gcccagtctc
    17761 aggcgttccg gtgatacctg cctgccagtt ccggccctgt aagtgaaata ccaggttaac
    17821 aggccgctgg tggaatgtct tacgctaaga ttgccaccgt cattaaaaaa ggcgtttttt
    17881 gtggttggtg atccgcttaa ttttcgtaaa agagtgtcgc taagtctatg aattgcgctg
    17941 ctcatgtttc gcgtttccgg tcatcaaaat taactacacc attgactaca ccgtgcatcg
    18001 tacaagggca aaccgtgacg aacaagagcg aacaaggaaa acaaaatttt cctttataat
    18061 catagcgatg aatgcacaca aaacactacc acgaacaccc gcgaacacta gatgataaat
    18121 aactatcatc tatcgttggc atcagcgaca tctgtcacat tcctgtcaat agcgttaact
    18181 gcttcaaatt tttgattcat acctgccccc tggcccctgt caaacgcttg acggctcgcc
    18241 ctaattctct aaattgtatt tctagagttg gcgaggtttt gaacagcccc ctctctgacc
    18301 ccggtttatt ccatcttact tgtataagat ttgcgaagga tgtcgaagca tggaaaaggg
    18361 tactgttaag tggttcaaca atgccaaagg gtttggtttc atctgccctg aaggcggcgg
    18421 cgaagatatt ttcgctcatt attccaccat tcagatggat ggttacagaa cgctaaaagc
    18481 tggacaatcc gttcagtttg atgtccacca ggggccaaaa ggcaatcacg ccagtgttat
    18541 tgtgcccgtc gaagtagaag cggcagtcgc atagctcttc tgtctcattg tgtacatcct
    18601 aaaggcaaaa tgccagcccg atcggctggc atttttatct caaaaattac tctcgtgcca
    18661 gagcatctac tggatccagt cgtgccgcat ttcgtgcggg taaccatcca aataaaatcc
    18721 cggtggccgt cgagcagaga aacgccagca gcagtgccaa cggtgaaaaa ccaatctccc
    18781 agccgggtaa gaaaagctgc aaggtgaaag caattaacag tgacagcgtt attcccaacg
    18841 cgccaccgac caggcaaacc agtacggctt cgatcaggaa ctgttgcaaa acatcgcttg
    18901 ctcgcgcacc tacagccatg cgaatgccaa tttcccgcgt ccgctcggtc actgacacca
    18961 gcataatatt cattacacca ataccgccca ccaccagcga aatcaccgcc accagcgtca
    19021 gaaacagttg taaagtacgt gtggtctttt caacagtttt caagacgccg tccatgttcc
    19081 aggtaaagaa atccttcttt ccgtggcgca gtgaaagtaa acgcgtgagt tgctgttccg
    19141 cctcggcgct gtcaaatcct tcttttaccc tgacagtaat ggagttcagc cacgactggc
    19201 ccataactcg cccggacatc gtgctgtaag gtagccagac acgcagcact ttactgctac
    19261 caaacatcga ctgtttttct tccgccacac caatgactct ggcgggcata ttgccgacca
    19321 gaatcacctc gccaaccaca tctgctttat gggggaaaag ctggcggcga gtattactgt
    19381 cgagaaccac gacctgcgca cgaccgttca gctgctcctg attaaaggtg tttccttcac
    19441 tgaaggtcat gccatagaca ttaaaataat cgccgctcac gccattggca ctggcagcaa
    19501 catcaacatt gttataacgc aggcgcaggt tttgcgagac ggcaggtgtg gctgaggcga
    19561 cccacggttg tttttggatg gcgattaagt cgtcgtactt cagcgcctgc tgatattgcg
    19621 gatcgtcatc gccaaaatct ttcccgggat agacatcaat agtattcgta ccaatagaac
    19681 gaatatccgc cagcaccatt tgtttggcgg cgtcacccac cacgacaatg gaaaccaccg
    19741 acgcaatacc gataataatc cccagcatgg tcagtaaagt acgcatttta ttcgctgcca
    19801 gcgcccgcca tgccatcgtc agcgcctcgt taaaaccgct gacaaactgc cgccagccag
    19861 acaccgtgtt gacaacaggc tccgtcccgc cagcaacatt cactttttca atggcgggag
    19921 gattgcgcac aatttcgccg tcgcgaattt cgatcacccg ctcggcctga gcagcgacct
    19981 gcggatcgtg ggtgacgata atcaccgtat gcccgcgatc gcgcagctga tgcaggatcg
    20041 ccatcacctc ttcgccagaa tggctgtcca gtgcgccggt tggttcatcg gcaagaatta
    20101 cctgaccgcc gttcatcaat gcccgcgcga tgctgacgcg ctgttgctga ccacccgaaa
    20161 gctgtgccgg ataatactct gtacggtctt ccagccccag tcgttgcagt aactcctggg
    20221 cgcgaagcag ccgctgtttc cgctcaagac cggcatagac ggcgggtact tcaacgttct
    20281 gctcggcggt taaatgcgaa agtaaatggt aacgctggaa aataaagccg aaatgctcgc
    20341 ggcgcagttg cgccagcgca tcggcgtcca gtgtggcaac atcctgaccg gcgacgcgat
    20401 aggtgccgct ggtggcctta tccagacagc cgagaatatt catcagggtc gatttaccgg
    20461 aacctgaagc gccaacaatc gctaccatct caccggcata aatatcgagg gtgatgctct
    20521 tcagcacctc aacctgctca tcaccggcag gatagctgcg acgaatatcc tttaattcga
    20581 gcaaaggcgt cattgtgcag ctcctggttt ggcctcacca atcaccactt catcgcccgc
    20641 ttccagccct ttgacaatct caacatcggt atcgttacgt gcgccaatcg tcacttcacg
    20701 ctcgcgtgtt tcaccattac gcaacaattt gactttataa cgattatcgc caaccggatc
    20761 gcctaacgcc gacagaggga tcgtcagcac atttttcaca tcggtgagct gaatatgcac
    20821 ttgcgcagtc atatccagcc gcagcaaacc attggggttg gggacttcaa aacgggcgta
    20881 atagaaaata gcgtcgttaa ccttttccgg cgtcggtagt acatccttga tttgcccctc
    20941 gtagcgcgtc agtggatcgc caagcaccgt aaaccaagct ttttgccccg gcttcaggtg
    21001 gattacatcc gcttcagaaa cctgcgcttt taccagcatg gtgctcatat ctgccagcgt
    21061 cagaatgttc ggtgcttgtt gtgcggcaat caccgtctgg ccttgcagag tggtgatttg
    21121 cgtgacttcc ccggccatcg gggcaacgat gcgagtgtaa tcgagattgg ttttagccgt
    21181 atcgagagaa gcctgattgc gcttgatttg cgcgtcaatg gtgccaattt gcgcctgttt
    21241 cacagccatc tccgtcgcgg cggtgtcgag ctcctgctgt gaaaccgcct gcgtttgtgc
    21301 cagacgttgc tgacgggaat acgtcacccg cgccagtttc agctccgctt ccgcctgctg
    21361 ccgctgcgca cgtagctcca tcagcgttgc ttcgacctcc ttaatctggt tttcagcctg
    21421 ttcaggatca ataaccccta aaagctggtc tttttttact ttatcgccaa tcgccaccga
    21481 cagagttttc aactgaccgc tgacctgcgc gcccacatca accttacgca gcgcgtccag
    21541 ctttccggtc gccagcacgc tttgctgtaa atcaccgggg cgcacaatca gtgtctgata
    21601 agtcggcacg ggtgcgttaa gaattctcca taacgtaatc agtccggcga tgactatcac
    21661 cagcgcaata acgtaacgct tcttcacggt tttccgcttt ttcataaata ttctgaatac
    21721 tccatatgcc aatccgggtc attaacttca acgaaatatc aacaaaaccc taatatttac
    21781 gttagtaatc acactattga tgtttggtta agatacgcaa tggtgaaatc gacgtcatgt
    21841 ataaccattg atttcatgaa tatgtcgcag ctaactgaac ggacctttac gccatctgaa
    21901 tctctcagca gcctgtcact ttttcttagt ctggcacgtg gacagtgtcg gccgggtaaa
    21961 ttctggcatc gccgtagttt tcgccagaaa tttttgctgc gctcgttgat tatgccgcgt
    22021 ttaagcgttg agtggatgaa cgaactttcc cactggccta atctcaatgt gttgttaacg
    22081 cgccagccgc gactgcctgt gcgtctgcat cgcccttacc ttgcggcgaa tcttagccgt
    22141 aagcaattgc tggaggcgtt acgttaccat tatgcgttac tccgcggatg tatgtcggcg
    22201 gaagaattca gcttatattt gaataccccc gggctgcaac tggcgaagct ggaaggcaaa
    22261 aacggcgagc agttcacgct tgagctgacc atgatgatct caatggataa agaaggtgac
    22321 agcacaatcc tgtttcgcaa cagcgaaggt attcctctgg cagagatcac gtttaccctg
    22381 tgtgaatatc aggggaaaag aacgatgttt attggcggac tgcaaggcgc aaaatgggaa
    22441 attccacatc aggaaatcca gaatgcgacg aaagcctgcc acgggctatt tcccaaacgc
    22501 ctcgtgatgg aagcggcctg tctgtttgcc caacgtttgc aggtagagca gattattgcc
    22561 gtcagcaatg aaacgcatat ttaccgcagc ctgcgttatc gcgataaaga aggcaagatc
    22621 cacgctgatt acaacgcttt ctgggaatcg gttggcggcg tatgtgatgc tgaacgccat
    22681 taccgccttc cagcacagat agcacgaaaa gagattgccg aaatcgccag taaaaaacgg
    22741 gctgaatacc gtcggcgcta tgagatgctc gacgctattc agccacaaat ggccacgatg
    22801 tttcgcggtt aatccgcgcg accgcgcgcc agccacagca cgcgtgagaa catttttttc
    22861 agcagcgtcg gtacggagtc cacaccacga cgtcctgcct ctattgccac ttcaatggca
    22921 agatcgggtt tggaagagcg atggatcgct ttcgagataa ttttgcgtag attcatcggt
    22981 acattttccg ggatttgcgc cacgcggtgg aacacatcgg aaaatccctg gcgatacata
    23041 aaatgttcca tatccagcgc cggtaacgcc gttaaatgtt ctcgttcggc ttcccgatcg
    23101 ttattcaaca ggctgcgtac cgtagcggca tatttcttcc ctgcttcatc gccatcgacc
    23161 agtacatgcc attcaatccc cattcggcgg gcaaatttaa ccagtggctt tagcccggac
    23221 tgggcaaact caatgacctt gatcccttcg gcatcaaaat gatgtccgca ctgacgcgcc
    23281 agttcattga taacccaggt ttccgtttcc ccttccacca gcaaccagca gcgtgcaaac
    23341 agcgatgacg gacggttaaa acgaatgtga aaggatatgc gtcggctatc ttcggtactc
    23401 aagccactcg gccccagacg ccaggcggca acgcgcgagg actcacgtac cagtcggcaa
    23461 acatgctcta ccggcgttaa cgaaagcaac tcacccgagt tggtggtggc aatgcgctgc
    23521 aatggcagaa gattcaacag atgccaggca actgaaagca taatggggtg taaacgggtt
    23581 tctggatctt cgatcagcaa caatggacgc gcgtctttat ccagtctcaa tgtgcctttt
    23641 gcctgcaata aagtagcaaa caggccgagc aaaataaccc gatacgagcg ccctccaggt
    23701 cggtcaatca tccggttgat gatgtccaga tagcgccagc ttcgttgctc attgctggcc
    23761 cgccgccgca ttaaacgata tcgcgcctgt ccggccccct gctcagagaa ataatgctca
    23821 agcagctgta ccattgcgga aagtccctga cgaatctgcc catcagagag attttgcgga
    23881 tgtgaggata actcacgggc gaggaaatcg agctggcgcg cggtgacttc cacattaggg
    23941 atatttggca ccgtgccgtt acgaatacgg cgcataaaac gggcatcacg caagcgcagc
    24001 accggcatta aacgcaccag atggcgtgcc tgatcgttaa tatcctcgac atcaatcgga
    24061 tgtccgtctt tatcgagaaa actgcgcagt gtcatcacgc tgccgtcttc cgcactctcc
    24121 ccttccagac gataaaaaat acggtgatag ccatcggtgc atggcgtcca gcacgcttcc
    24181 agcggccgat aacggcgaac ccgatgtcgg cctggcagcg attcgcggaa ggtcaaaata
    24241 atatgcagat gatgttctcg cccgttgata tctcccggcg ggaaccagaa atcgtcgcgc
    24301 tcaaaatggt agagatctga ttctggcgat agcagcaggg ttaaggcgtc cagcaagctg
    24361 gatttacccc acgcgttctc cccaatcagg acgttgtttt gttccagcat caacgacaaa
    24421 cggttgatac cgcgaaaacc cacaatttca acgcgctcaa gaatcatatc ccctccagaa
    24481 atatgtgact tatcctttac gctggcagta taacgggaaa ggccttgcta atacgtgatg
    24541 acatcacatt taagcatggc aaatgtctga aaaatgagcg aataatcgcg ttatactccc
    24601 tcttgctgat aagtctgcaa aaacaacaaa atcaaataat tagcatgata tattattttt
    24661 tattacgcct aaaggattaa atcttttgcg tgcactacac tgtcaatcag acaaatgact
    24721 atatggggca acccgctgtt gccctaaatc aaatttactg gatttattta tctggtgaat
    24781 gactcgcctt cgtttttaaa atagccacgc attatttccg tcgtcatttt tttacgacga
    24841 ccctataaaa cgaccatatt tttcacaggg tcaattttta attgaggtgg atatgttcag
    24901 aaaattagca gctgaatgtt ttggtacttt ctggcttgtt tttggtggct gtggtagtgc
    24961 tgtactggcc gcaggcttcc cggaattagg cattggtttt gccggcgtgg cgttggcgtt
    25021 cggtctgacc gttctgacga tggcctttgc tgttagtcat atttctggtg gtcattttaa
    25081 cccggcggtc actattggtt tatgggctgg cggacgtttt ccggcaaaag aagtcgttgg
    25141 ctacgtaatt gcccaggttg tcggcggtat tgttgcagcg gcgctgctgt atttaattgc
    25201 cagtggtaaa acgggttttg acgcggcagc cagcggtttt gcttctaacg gttatggcga
    25261 gcattcacca ggcggttatt ccatgctttc cgcgctggta gttgaactgg tattgagtgc
    25321 aggtttcctg ttggtgatcc acggcgcaac cgccaaattc gcgccggcag gttttgcgcc
    25381 gatcgctatt ggtctggcct taaccctgat tcacttaatt agtattccgg tgactaacac
    25441 ttctgttaac ccggcgcgca gcaccgcggt tgctatcttc cagggcggct gggcattaga
    25501 acaactgtgg ttcttctggg tggtgccaat tgtcggcggc attatcggtg gtctgattta
    25561 ccggaccctg ctggaaaagc gtgattaatt aacgcagaag gcctggcgta atgtcaggcc
    25621 tttttttaag atgttgttat atgttgattc atcatcttgt tgcgctcctt tcatgagcta
    25681 agccacatat tgccactggc gcaaggagcg cgcagggggc ggccaatcgc cgccgccccc
    25741 tgcccccccg ggctctggcg aacaaaatcg ccgctgcgcg gtgccctcga cttatccctt
    25801 acggctaccg ggtcgggcgc gaggtaacat ccctgtaaaa cgcgccctca gcccacatcc
    25861 atgtgggctg ccccggcctt cagggaacgc ctcggcgatt ttgacgccac caacatcggt
    25921 gcagtttgta attaaagcgc aagaatatct ttaaatgaaa acgaatactc tactgctcct
    25981 tatgcataac atttcctttt aattgcaact ttactcgtcc ttccgctttc agtagtgtgt
    26041 catcgcgcat ttcgacttca tctttgcaag gactgggttt tcatgttttc tgggctgtta
    26101 atcattctgg ttcccctgat tgtgggttac ctcattccgc ttcgccaaca agcttcgtta
    26161 aaagttatta atcagctatt aagctggatg gtttacctta ttctcttttt tatgggtatc
    26221 agtctggcgt ttctcgataa cctcgccagt aacctgttgg cgattctgca ttattctgcc
    26281 gtcagtatta ccgttatttt actgtgtaat attgccgccc tgatgtggct ggagcgaggc
    26341 ctgccgtggc gcaaccacca tcagcaagaa aaactcccgt cgcgtattgc gatggcgctg
    26401 gagtcgctaa aactgtgcgg cgtagtagtg attggttttg ccattggtct gagtggactg
    26461 gctttcttac aacacgcgac cgaagccagt gaatacacgt taattttgct acttttcctc
    26521 gttggtattc agttgcgcaa taatggcatg accttaaagc agattgtcct taatcgccgg
    26581 ggaatgattg tcgccgtggt ggtggttgtc agttcattaa ttggtggttt aattaacgcc
    26641 tttattcttg atctccccat caataccgcg ctggcaatgg cctccggttt cggctggtat
    26701 tctctttccg gtattttatt gaccgaatct tttggtccgg taatcgggag cgcggcgttt
    26761 tttaatgatc tggcccgtga actgattgct attatgttga tccctgggct gattcgccgc
    26821 agccgctcta ctgcactggg cttatgcggt gccacatcaa tggatttcac cctgcccgtt
    26881 cttcaacgta ctggcgggct ggatatggtc ccggcggcaa ttgttcacgg ttttattctt
    26941 agcctgttag tgccgatcct catcgccttt ttctctgcgt aatacctctc tggcggtaga
    27001 tccctgccgc caaaattgcg ctaaatcaat ctcccttaaa gttgcatgaa aaatcccttt
    27061 tatccccgcg ttaagcgtct taaccttaaa catgtatatt aaatataact ttaaaaggtg
    27121 tgatcatgtt ttgtgtgcaa tgtgaacaaa ctatccgtac tccggcagga aacggctgct
    27181 catacgcgca ggggatgtgt ggtaaaacgg cggaaacttc tgaccttcag gatttactca
    27241 tcgcggcgct gcaagggctt tctgcctggg cggtaaaagc gcgtgaatac ggcatcatca
    27301 accacgatgt tgacagcttc gcgccacgtg cctttttctc aaccctgacc aacgttaact
    27361 tcgattctcc tcgtattgtc ggctacgctc gtgaagcgat tgccttgcgc gaggcgctga
    27421 aagcacaatg cctggctgta gatgccaacg cgcgcgtcga taacccgatg gctgacctgc
    27481 aactggtgag cgatgatctc ggcgagctgc aacgtcaggc agcagaattt acccctaaca
    27541 aagataaagc ggcgattggc gaaaacattc tcggcctgcg tctgctgtgc ctgtatggcc
    27601 tgaaaggtgc ggcggcctat atggaacacg cgcatgttct cggtcaatac gacaacgata
    27661 tttatgccca gtaccataaa atcatggcgt ggctggggac ttggcctgcc gatatgaacg
    27721 cgcttcttga gtgttcaatg gaaatcggcc agatgaactt caaagtgatg agcattctgg
    27781 atgcaggcga aaccggtaaa tacggtcacc cgacgccaac ccaggtcaac gtcaaagcga
    27841 cggcgggtaa atgcattctg atttccggtc acgatctcaa agatctctac aacctgctgg
    27901 aacagaccga aggcacgggc gttaatgtct acacccacgg cgaaatgctg cctgcgcatg
    27961 gctacccgga gctgcgtaaa ttcaagcatc tggtcggtaa ctacggcagc ggctggcaga
    28021 atcagcaagt ggagttcgct cgtttccctg gccccatcgt gatgacctcg aactgcatca
    28081 tcgacccaac cgtaagcgct tatgacgatc gtatctggac ccgcagcatt gttggctggc
    28141 ctggcgtgcg tcatctggac ggtgatgatt tctctgcggt tatcacccag gcgcaacaga
    28201 tggcaggctt cccgtacagc gaaattccgc accttatcac cgtgggtttt ggtcgccaga
    28261 cgctgcttgg cgctgctgat acgctgattg atctggtgag ccgtgaaaaa ctgcgtcata
    28321 tcttcctgct tggtggctgt gacggcgcac gcggcgagcg ccactacttc accgatttcg
    28381 ccaccatcgt gccggatgac tgcctgatcc tgaccctcgc ctgtggtaaa tatcgcttta
    28441 acaaacttga gtttggcgat atcgaaggtc tgccgcgtct ggtagatgca ggtcaatgta
    28501 acgatgctta ctcagcgatt attctggctg tcactctggc agagaaactg ggctgtggtg
    28561 tgaacgatct gccgctgtcg ctggtgctct cctggtttga acagaaagcg atcgtcattc
    28621 tgctgacgct gctttctctg ggcgtgaaaa atatcgtcac cggcccgact gcacctggtt
    28681 tcctgacacc agacctgctg gcggtgctga acgagaaatt cggcctgcgt tctatcacca
    28741 ctgttgaaga agacatgaag caactgttga gcgcgtaagg agtttgtcga tgacgatgcc
    28801 aacgaatcaa tgcccgtggc ggatgcaggt tcatcacatt acgcaagaaa cgccggatgt
    28861 gtggacgatt tccctgattt gccacgatta ctacccatat cgcgccgggc aatatgcact
    28921 ggtcagcgtg cgaaactcag cggaaacgct gcgtgcttac accatttcct ccacgccagg
    28981 cgtgagtgaa tatatcaccc tgaccgtgcg gcggattgat gacggtgtcg gctcccagtg
    29041 gctgacgcgc gatgtaaaac gcggtgatta tctctggctt tcggacgcga tgggggaatt
    29101 tacctgcgac gataaagcag aagataaatt cctgttactg gcggcaggct gcggcgtcac
    29161 gccgattatg tcgatgcgtc gctggcttgc gaagaaccgt ccacaggccg atgtgcgggt
    29221 gatctacaac gtgcgtacgc cgcaggatgt tattttcgcc gatgagtggc gtaactatcc
    29281 ggtaacgctg gtggcagaaa ataacgttac cgaaggcttt atcgctggtc gtctcactcg
    29341 cgaactgctg gcaggtgtac ctgacttagc ttcacgtacc gtgatgacct gcggcccggc
    29401 tccgtatatg gattgggtag agcaggaagt gaaagcgctc ggcgtgacgc gtttctttaa
    29461 agagaaattc ttcaccccag tagcagaagc ggcgaccagc ggtctgaaat tcaccaaact
    29521 gcaaccggca cgagaatttt acgccccggt tggcaccacg ctactggagg cgctggaaag
    29581 caataacgtt ccggttgtcg ctgcctgccg tgcgggtgtt tgcggctgct gtaagacaaa
    29641 agtggtttcc ggtgaatata cggtgagcag tacaatgacg ctgaccgacg ccgaaatcgc
    29701 tgaaggttac gtactggcct gctcctgcca tccgcagggg gatttggttc tcgcataatc
    29761 gccttatgcc cgatgatatt cctttcatcg ggctatttaa ccgttagtgc ctcctttctc
    29821 tcccatccct tccccctccg tcagatgaac taaacttgtt accgttatca cattcaggag
    29881 atggagaacc atgaaacaaa cggttgcagc ttatatcgcc aaaacactcg aatcggcagg
    29941 ggtgaaacgc atctggggag tcacaggcga ctctctgaat ggtcttagtg acagtcttaa
    30001 tcgcatgggc accatcgagt ggatgtccac ccgccacgaa gaagtggcgg cctttgccgc
    30061 tggcgctgaa gcacaactta gcggagaact ggcggtctgc gccggatcgt gcggccccgg
    30121 caacctgcac ttaatcaacg gcctgtttga ttgccaccgc aatcacgttc cggtactggc
    30181 gattgccgct catattccct ccagcgaaat tggcagcggc tatttccagg aaacccaccc
    30241 acaagagcta ttccgcgaat gtagtcacta ttgcgagctg gtttccagcc cggagcagat
    30301 cccacaagta ctggcgattg ccatgcgcaa agcggtgctt aaccgtggcg tttcggttgt
    30361 cgtgttacca ggcgacgtgg cgttaaaacc tgcgccagaa ggggcaacca cgcactggta
    30421 tcatgcgcca caaccagtcg tgacgccgga agaagaagag ttacgcaaac tggcgcaact
    30481 gctgcgttat tccagcaata tcgccctgat gtgtggcagc ggctgcgcgg gggcgcataa
    30541 agagttagtt gagtttgccg ggaaaattaa agcgcctatt gttcatgccc tgcgcggtaa
    30601 agaacatgtc gaatacgata atccgtatga tgttggaatg accgggttaa tcggcttctc
    30661 gtcaggtttc cataccatga tgaacgccga cacgttagtg ctactcggca cgcaatttcc
    30721 ctaccgcgcc ttctacccga ccgatgccaa aatcattcag attgatatca acccagccag
    30781 catcggcgct cacagcaagg tggatatggc actggtcggc gatatcaagt cgactctgcg
    30841 tgcattgctt ccattggtgg aagaaaaagc cgatcgcaag tttctggata aagcgctgga
    30901 agattaccgc gacgcccgca aagggctgga cgatttagct aaaccaagcg agaaagccat
    30961 tcacccgcaa tatctggcgc agcaaattag tcattttgcc gccgatgacg ctattttcac
    31021 ctgtgacgtt ggtacgccaa cggtgtgggc ggcacgttat ctaaaaatga acggcaagcg
    31081 tcgcctgtta ggttcgttta accacggttc gatggctaac gccatgccgc aggcgctggg
    31141 tgcgcaggcg acagagccag aacgtcaggt ggtcgccatg tgcggcgatg gcggttttag
    31201 catgttgatg ggcgatttcc tctcagtagt gcagatgaaa ctgccagtga aaattgtcgt
    31261 ctttaacaac agcgtgctgg gctttgtggc gatggagatg aaagctggtg gctatttgac
    31321 tgacggcacc gaactacacg acacaaactt tgcccgcatt gccgaagcgt gcggcattac
    31381 gggtatccgt gtagaaaaag cgtctgaagt tgatgaagcc ctgcaacgcg ccttctccat
    31441 cgacggtccg gtgctggtgg atgtggtggt cgccaaagaa gagttagcca ttccaccgca
    31501 gatcaaactc gaacaggcca aaggatttag cttgtatatg ttgcgcgcaa tcatcagcgg
    31561 acgcggcgat gaagtgatcg aactggcgaa aacgaactgg ctaaggtaaa aagggtggca
    31621 tttcccgtca taataaggac atgccatgat tgatttacgc agtgataccg ttacccgacc
    31681 gagccgcgcc atgctcgaag cgatgatggc cgccccggtt ggggacgacg tttacggaga
    31741 cgaccctacc gttaatgctc tgcaggacta tgcagcagag ctttccggta aagaagccgc
    31801 catttttctg cctaccggca ctcaggccaa cctggtcgct ctgctcagtc actgcgaacg
    31861 tggcgaagag tatattgtcg gtcaggccgc gcataactat ctgtttgaag ccggtggcgc
    31921 agcggtgctg ggcagtattc agccgcaacc catcgacgcg gctgccgacg gcacgctacc
    31981 gctggataaa gtggcgatga aaatcaaacc cgacgatatc catttcgccc gcaccaaatt
    32041 actcagtctg gaaaacaccc acaacggcaa agtgctgccg cgtgaatacc tgaaagaagc
    32101 atgggaattt acccgcgagc gcaatctggc gctgcatgtt gacggtgcgc gcatctttaa
    32161 tgccgtggtg gcttacggct gcgaactgaa agagatcacg caatattgtg attcgttcac
    32221 catttgcctg tcgaaaggtc ttgggacgcc agtcggttca ttactcgtcg gtaatcgtga
    32281 ttacattaaa cgtgccatcc gctggcggaa aatggccggt ggcgggatgc gtcagtccgg
    32341 gattctggct gccgccggga tgtatgccct gaaaaataac gtcgcacggt tgcaggaaga
    32401 tcacgacaac gccgcctgga tggcggagca actgcgtgaa gcaggcgcgg atgtgatgcg
    32461 tcaggacact aatatgctgt ttgttcgcgt cggggaagaa aatgctgccg cgttaggcga
    32521 atacatgaaa gtgagaaacg tgctgattaa cgcctcgccg attgtccgcc tggtgacgca
    32581 tcttgacgtc tcgcgcgaac aactggcgga agtcgccgcc cactggcgcg cattcctggc
    32641 gcgttaagga gagagacgtg ccgcaacgca ttttagttct cggtgccagt ggctacattg
    32701 gtcagcatct ggtgcgcaca ctcagccagc aagggcatca gatcctggcg gcagcacgtc
    32761 atgtcgacag gcttgcaaag ctgcaactgg caaacgtcag ttgccataaa gtcgatctca
    32821 gctggccgga taaccttccg gccctgttgc aggatatcga taccgtctat tttctggtgc
    32881 acagcatggg cgaaggcggc gattttatcg ctcaggagcg ccaggtggct ctcaacgtcc
    32941 gcgatgcgct acgtgaagta ccagttaagc aattaatctt tctcagttcg ttgcaggccc
    33001 cgccacatga gcagtcggat catctgcgtg ctcgtcaggc tacggcggac attcttcgtg
    33061 aagcgaatgt acctgtgacc gaacttcggg ccggaattat cgttggcgca ggttcagcgg
    33121 cgttcgaagt catgcgcgat atggtctaca acctgccagt gttaacgccg ccacgctggg
    33181 tacgttcacg caccacgccc atcgcgctgg aaaacttgct gcactatctg gtggcgttgt
    33241 tagatcatcc ggccagcgaa caccgcatct tcgaagccgc cggaccagag gtgctcagtt
    33301 atcagcaaca gtttgaacat tttatggcgg tgagcggtaa gcgccgctgg ttgatcccca
    33361 tccccctccc cacccgctgg atttcggtgt ggtttctcaa tgtgattact tccgtaccgc
    33421 ccaccaccgc cagggcgttg attcaggggc tgaaacacga tctgctggcg gatgataccg
    33481 cgctacgtgc actcatccca caacggctga tcgctttcga tgacgcggta cgtagcacgt
    33541 tgaaagagga ggaaaaactg gtcaactcca gcgactgggg ctacgacgct caggcctttg
    33601 cccgctggcg accggagtac ggttattttg ccaaacaggc ggggtttacc gttaaaacgt
    33661 ccgccagcct tgctgcttta tggcaggtag tgaaccaaat cggcggtaaa gagcgttatt
    33721 tctttggcaa tattttgtgg cagacacggg cgttgatgga ccgcgcgatc ggtcataagc
    33781 tggcgaaagg ccgcccggag cgcgaatatt tacagactgg cgatgcggta gatagctgga
    33841 aagtgattgt cgttgaaccg gaaaaacaac ttacgttgtt atttggcatg aaagcgccgg
    33901 ggctgggacg actgtgtttt agcctggaag ataaaggcga ctatcgtact atcgatgtcc
    33961 gcgctttctg gcatccgcac ggtatgccgg ggctgtttta ctggttattg atgatccccg
    34021 cgcatctgtt tatttttcgc ggaatggcaa aacaaatcgc cagactggca gaacaaagca
    34081 cagattaatc gacaaaacct gcgttattct ttcatgtttc ccattgcatc atgggggaaa
    34141 tcacggaaga atgcgcacgt tattttctca acaacagtgg attgatatga aggtactggt
    34201 taccggcacc accagcggct taggtcgaaa cgcggtagag tttttatgcc agaaaggcat
    34261 cagcgtgcga gcgaccggtc gcaacgaggc aatgggcaaa ttgctggaaa aaatgggcgc
    34321 agagtttgtt ccggcggatc tgaccgagct ggtttcatca caagctaaag tgatgctcgc
    34381 gggcattgat acgctgtggc actgctccag ctttacctca ccctggggga cacaacaggc
    34441 tttcgatctg gctaacgttc gcgccactcg ccgcctgggt gaatgggctg tcgcctgggg
    34501 tgtgcgtaac tttattcata tctcttcccc ctccctgtac ttcgattatc accaccatcg
    34561 cgatattaaa gaagattttc gccctcaccg cttcgccaac gagtttgccc gcagcaaagc
    34621 agccagcgaa gaagtgatca atatgttttc gcaggcgaat ccacaaacgc gctttactat
    34681 tctgcgccca caaagtctgt tcggaccgca cgataaagtc tttattcccc gtctggcgca
    34741 tatgatgcac cactacggca gtattctgtt accgcatggc ggcagtgcgc tggtcgatat
    34801 gacctactat gaaaatgccg tgcacgcaat gtggctggca agccaggaag cctgcgataa
    34861 gctaccttcc gggcgtgtgt acaacatcac caacggcgag catcgcacac tgtgcagcat
    34921 cgtgcagaag ctgatcgacg agttgaatat tgactgtcgt attcgttccg tcccctaccc
    34981 gatgctggat atgatcgccc gcagcatgga gcgtttaggc cgcaagtcag caaaagagcc
    35041 gccgctgacc cactacggcg tctccaagtt gaattttgac tttacgctgg atattacgcg
    35101 tgcgcaggaa gagttaggtt atcagccggt catcaccctg gatgaaggta tcgagaaaac
    35161 tgccgcctgg ctacgcgacc acggaaaact gccgcgctaa tcctgcccgt atttctccag
    35221 caatgcttcg gcaatcgcct gagtttctgc atccgcttcg ccgttatata acgtcgggcg
    35281 gaaatgcatc tggaatgcca taatcacgcg ccgctgctcg cgcggtgtca tatcaggttt
    35341 aacgtcataa ccgtagcgcg ccaaaagctc cagcaatgac gcggtatcta ccggtgtgtg
    35401 cggcgcgcgc ccggcaaggt aaaagttaac ccgctgcgca tccggccaag caccaatccc
    35461 ctgctgcgcc agttgctgcc agggaaataa tggccccgga tcgtctttgc gctgcggtgc
    35521 gatatccgca tgtgccacta cgttttccgg cttgatgtga taacgggcaa taatatcttt
    35581 cgccagtgga ataagcgcct gaatctgtgc cggttcaaac ggggcaaaat atttcactcc
    35641 ggccgatttt tgccagccac ggttttccag ctcaatgcca atagaggtgt cgttaaggcg
    35701 cgttgccccg cgccaggcgc taatccccgc atgccaggcc agttcttgtt ccggcaccag
    35761 ttgccagatg cgcggtttac cgttgtatcg cggtggtacc gcagggacca gataatgcga
    35821 gctgacctgc ttatcggtca gtgtcgccag cgagctatca aaatcatctg cggtgtagtg
    35881 gatcaccagc actttaatgc gcggatacgc cgcctgcgcc tggcgtcggg tatcaagctg
    35941 atatccctct ttctcgacaa tgcctttttc gcctgcacac cctgccaata acagagcggc
    36001 agcgaccagc cagaagactc ttctcatcga cgcgttttca ccgccgtacc gctgacgcta
    36061 accatcagca tactgccgtt ttgcccgacc gtttcgtagt cgatatcaat accgacgacg
    36121 gcatcggccc ccagcgcccg cgcctgggag cctaattcct caaaggcgat ctcccgtgct
    36181 ttacgcagtt ctttttcata cgcaccggaa cgtccgccaa cgatatcgcg gataccggca
    36241 aagaaatcac ggaaaatatt ggcacctaaa atcgcttcgc cggtcaccac accgcaatat
    36301 tcaacgatgg tctggccttc cagagttggg gttgtagaaa attgcatgat cgatctcctt
    36361 gtttgctaag ccttcgatct caaaagcatt atcagactga tacgctatta ttgaaaggat
    36421 atcattatta tcgtagaacg ctttcagagc gatcgcttat aaggaaatca ttatgcgcta
    36481 cagcaaattg acaatgctta tcccctgcgc actgctcctc agtgcctgca ccacagtcac
    36541 tccagcttat aaagataacg gcacacgcag tggtccttgc gttgaaggag gcccgggtaa
    36601 cgtggcccag caattttatg actaccgcat tttgcaccgc agcaacgaca tcaccgcctt
    36661 gcgcccttat ctgagcgaca aactggcgac actgcttagc gatgcctccc gggataataa
    36721 ccatcgggaa ctactgacca acgatccatt ctccagtcgc acaaccttgc cagagagcgc
    36781 ccgtgttgcc agtgcctcaa caatcccgaa tcgtgatgcg cgaaatattc ctctgcgtgt
    36841 ggatctaaaa cagggtgatc agagctggca ggatgaagtg ttaatgattc aggaaggcca
    36901 gtgttgggtg atcgacgatg tacgctactt aggcggcagc gttcatgcca cggcgggtac
    36961 gctccgtcag tccatcgaga atcgctaacg ccttgaacaa ccaataaacc ccgtaaattg
    37021 tccggcattt cgtgaggaat gccgacattt atgctcgccg accaccgccc ccgttatttt
    37081 gtgctatgtt tattgaataa tgcgctttgc ttttaacttt taaagcagaa atattgcata
    37141 attattctgt caacggtact atcttcgacc tcaatagcta tcagactgcc agtatacgag
    37201 tgtcaatgag tattcaatta aacggcatta attgcttcta cggcgcgcat caggcgctgt
    37261 tcgatatcac gctggattgc ccacagggcg aaacgctggt gttacttggc cccagcggcg
    37321 cgggtaaaag ctcgctgctg cgtgtactca atctgcttga gatgccgcgc tccggtacgc
    37381 tcaacattgc aggcaaccat ttcgatttca ccaaaacacc ctctgacaaa gcgattcgcg
    37441 atttgcgtcg taacgttggc atggtgtttc agcaatacaa cctgtggccg catctgaccg
    37501 tgcagcaaaa cctgattgaa gcgccctgcc gtgtactggg gttgagtaaa gatcaggcgc
    37561 tggcccgtgc agaaaaactg ctggaacgtc tgcgtctcaa accttatagc gatcgttacc
    37621 cgctgcatct ttctggtggt cagcagcagc gtgttgctat tgcccgtgcg ttgatgatgg
    37681 aaccgcaggt actgctgttc gatgaaccga ccgccgcact ggacccggaa attacggcac
    37741 aaatcgtcag catcattcgt gagctggcag aaacgaatat tacccaggtg atcgtcaccc
    37801 acgaagttga agtggcgcgt aaaaccgcca gccgagtggt gtatatggaa aatggtcata
    37861 tcgtagaaca aggcgacgcg agctgcttta ccgagccgca aaccgaagca tttaaaaact
    37921 atctctctca ctaagattcg ggataacaac aatgaaaaaa gttctgattg ccgcgttaat
    37981 tgcaggtttt agtctttccg ccacagctgc cgaaaccatt cgttttgcta ccgaagcatc
    38041 ctatcctccg tttgaatcga ttgatgcaaa caaccagatc gttggttttg acgtcgacct
    38101 ggcacaagcg ctgtgtaaag agattgatgc aacctgcact ttctctaacc aggcgtttga
    38161 cagcctgatc ccaagcctga aattccgtcg cgtagaagcc gtgatggcgg gcatggatat
    38221 cactccggag cgtgaaaagc aggtgctgtt taccaccccg tactatgaca actctgccct
    38281 gtttgtgggt cagcaaggca aatacaccag tgttgatcag ctgaaaggca aaaaagtcgg
    38341 cgtacagaac gggacgacac accagaaatt cattatggat aagcacccgg aaatcaccac
    38401 cgttccgtat gacagctacc agaacgcaaa actggatctg caaaacgggc gtatcgacgg
    38461 cgtcttcggt gacaccgcag tggtcactga gtggctgaaa gataacccga aactggcggc
    38521 ggtgggcgac aaagtgaccg ataaagatta cttcggcact ggcctcggca tcgcggtacg
    38581 tcagggcaac actgagctgc agcagaaact caacactgcg ctggaaaaag tgaagaaaga
    38641 tggcacttac gaaaccatct acaacaaatg gttccagaag taattcctga tgaatgaatt
    38701 ttttccttta gcaagcgccg ccgggatgac cgtcggcctt gccgtttgtg cattgattgt
    38761 cgggctggcg ctggcgatgt tctttgcggt atgggagtcg gaaaaatggc gtcctgtcgc
    38821 gtgggcaggt tcagcgctgg taaccattct gcgtggcctg ccagaaattc tggtggtgct
    38881 gtttatctat tttggctcct cgcagctgct gctgacgctt tcggatggct tcactatcaa
    38941 tcttgggttc gtgcagatcc cagtgcagat ggacattgag aacttcgacg ttagcccgtt
    39001 cctttgtggt gtcatcgctc tgtcactgct gtatgccgcc tatgcctcgc aaacgcttcg
    39061 gggcgcgttg aaagcggtgc cggtgggtca gtgggagtct ggtcaggcgc tggggctgtc
    39121 gaaatcggct atctttttcc gtctggtgat gccgcagatg tggcgtcatg cgctgcctgg
    39181 cctcggtaac cagtggctgg tgctgctgaa agataccgcg ctggtcagtt tgattagtgt
    39241 gaatgattta atgctgcaaa caaaaagcat cgctactcgt acccaggaac catttacctg
    39301 gtacattgtg gcggcggcga tttacctggt gatcaccctg ctcagtcagt acattctcaa
    39361 acgcattgac ctgcgcgcga cacgttttga gcggaggccc agctaatgtt tgagtattta
    39421 cccgaactga tgaaagggct acacaccagc ctgacgctaa ccgttgcctc gctgattgtg
    39481 gcactgattc tggcattgat ttttaccatc atcctgacgc tgaaaacgcc ggtgctggtg
    39541 tggctggtgc ggggttatat cacgctgttt accggtacgc cgctgctggt gcagatcttc
    39601 ctgatttatt acgggccggg ccagtttccg actttgcagg agtatccggc actgtggcat
    39661 ttgttgtcag aaccgtggtt atgtgcgctg attgcgttat cgctgaacag tgcggcgtat
    39721 accacgcagc tgttttacgg tgcaattcgt gcgatcccag aaggtcagtg gcagtcctgt
    39781 agcgccctgg gaatgagcaa aaaagatacg ctggcgatcc tgctgccgta tgcctttaaa
    39841 cgctcgctct cttcttattc caacgaagtg gtgctggtgt tcaaaagtac ctctctggct
    39901 tacaccatta cgctgatgga agtgatggga tacagccagt tgttgtacgg acgcacctac
    39961 gatgtaatgg tgttcggtgc ggcagggatt atttacctgg tcgtcaacgg cctgctgacg
    40021 ctgatgatgc gtctgatcga gcgcaaagca ctggcattcg aacggcggaa ttaatgtagc
    40081 tgcgtacatt cgccgggggg tttgtaggtc tgataagacg cgccagcgtc gcatcagaca
    40141 tcagcacggt gcctgtgccg gatgcggcgt ggacgcctta tccggcctac atagtgcata
    40201 aattcttata atgaagacgg acaactcact aagttatccg tcttttttta tttcatttaa
    40261 attatttaat catatttatt gcatataaat tcacttgatg acattattat cccatgccgc
    40321 agacacggcc aaaaatcata agattgacag acgggagttc catcatgaaa aagttagttc
    40381 ttgccgcttt acttgcttcc tttactttcg gtgcttctgc cgcagagaaa atcaattttg
    40441 gcgtttcagc cacctatcca ccctttgaat ctataggtgc taataatgag attgtcggct
    40501 ttgatatcga tctggcaaaa gccttgtgca aacaaatgca ggcagaatgt acttttacta
    40561 atcacgcgtt cgacagcctg atcccgtcac tgaaattcag aaaatatgac gccgtaatct
    40621 ccggtatgga tatcacgccc gagcgtagca agcaggtatc ttttaccacg ccctactatg
    40681 aaaactcagc cgtcgtgatt gccaaaaaag atacctacaa aacgtttgcc aatctgaaag
    40741 gcaaacgtat tgggatggaa aacggcacca cgcaccagaa atatattcag gatcagcacc
    40801 ccgaagtgaa aactgtctcc tatgacagtt atcagaatgc ctttatcgat ctgaaaaatg
    40861 gtcgtattga tggggtattt ggtgacacag cggtggtaaa cgaatggctg aaaaccaatc
    40921 cgcaactggg cgttgctact gaaaaagtga ccgatccgca atacttcggc accggtctgg
    40981 gcatcgctgt acgtccggat aacaaagctc tgctggaaaa actgaataac gcgctggcag
    41041 caattaaagc tgacggtaca tatcagaaaa tcaatgacca gtggttcccg cagtaattga
    41101 gactgatgac aaaagtaaga ttgcctgatg cgctgcgctt atctggccca catcaactct
    41161 gcaatgtatt gatttagcat gattttgtag gccggataag gcgtacacgc cgcatccggc
    41221 atgaacaaag cgcacctgat cagcaatctg aagccgcacc tgcggctttt ttattgcttc
    41281 accagcagcg tcagcacttc atagtgcgcg gtgtgcggga acatatcaaa aagctgtacc
    41341 cgttcgatgc gaaacccagg cagttcgcgg atatctttcg ccatagtttg ggcgttacag
    41401 ctggagtaga tgataaaacg cggtgccatc gttgagagat aatcacacag cggtttacca
    41461 atgccgcggc gcggcgggtt aaccagcacc agctccggca cctccccctg agcagtggca
    41521 aactgagtgg agtccagcgc ctgaaattgt aaacgagtta agcccagttc agcggctgac
    41581 tgctttgcac aggcaatggc ctctggcgca atttcgatcc cggttaactg catgtcaggc
    41641 gtcgcgcagt gtaaaccaaa gccccccaca ccgcagaaca gatcccacat atgtttaact
    41701 ggcagctgcc gtacccagtc gcgcgcggta gcgtacagct ggctggcgac cgccggatta
    41761 gtctggaaga aactttgcgg acggatccac agcggtacgt cattaaaacg ctctgccagc
    41821 gcctgttgtt cagtcaggta gatctccgtc tccccttcca taatcgccat atgtaccggc
    41881 tgaatattga cggtaataac tttcagttgc ggtagttgtt cctgtaacca cggcagcgcc
    41941 ttacgcagtt gtgccagttt ggtttcagaa cgcagtacaa agcgcagcat catgccgcca
    42001 tcgctctggc tttcagtcag cagaatgtat ttcagttcgc cacgcttacg cgccacgttg
    42061 tagggcgtta accccgcaca ggcgataaac ggttttagcg ccgcaaaaac gggcgcaaaa
    42121 gaggcaggat aaagcgggca gtcacaaagg tcttccggcg tgccatcgcg atgcagcata
    42181 ccgagcagtg gtttttcaac gctaccactc accaccattt tggctttatt acgaaaccct
    42241 tgttccgggc ctgacaccgg cgcgcaccat tcctcaaccg gaaaatcggc gagcagattt
    42301 ttaagatcgg cggttttagc ggagagttgc tctggaatcg gctgcgttat ccactgacag
    42361 gaacgacagc gacccgcgtc gtaaagtgcg cactgcatac attgaccttc acatcatcag
    42421 gggcgacgat tatacacatt attgcaactg gaagaaccgc cgactggtcg agggaacgaa
    42481 cagcagcatc aggatgagca tatccggcag cttctgcagc atcaggctat ggaagatttc
    42541 acgttttgat tcaccgggaa tgctgaacag ctccggataa ccatacccca gcgaagccgc
    42601 ccacaagtaa cttgcggcgg tgatttgtgt cagcagatat agccagcgcg cccagcgacg
    42661 ccctttcacc agtgaaaacg cacaccagat ctcgataaaa accagcacca gactgcttaa
    42721 aaataccagc gttaagctcc aggtttgtac gctgcgatga atgaactcgc caataccgcg
    42781 cacgcccagc gtattgaaaa tcatcagcac gtcgaggcca cggatcataa taatggcgag
    42841 cgccgctacc tgcaccagcg caggcacatt caggcgagca tgagatgaag atgttttctt
    42901 aaaaaatccc aacgtttcgt cttccatgaa aacaatgccg cggcatgcgc ggcattatgt
    42961 agccaggttg gcaaatttta gtgtcttcag ccatgtcttg cacgctggat atcgcgtatc
    43021 cgctgttttt ctgcgcgagc catcagatac caggcgataa atccgacaat tccgaccgca
    43081 ccgagaatta acgttgcaag ggcgttgatt tccggattca cccccatccg cacgctggaa
    43141 aagaccagca tcggtaacgt ggtggctccc ggcccggaaa caaagctggc gatcaccaga
    43201 tcatcaagcg acaaagtaaa agccagtaac cagccagaaa tgatcgcagg catgatcatc
    43261 ggtagcgtaa tgacgaaaaa cactttcagc ggcatcgcac cgagatccat cgctgcttct
    43321 tctatcgagc gatccagttc ccgcagacgc gacgaaataa cgaccgccac ataagccgta
    43381 cagaaagtga catgcgccag ccagatggta agcataccgc ggtccgcagg ccagccaatg
    43441 gcatgagcaa gcgcgacgaa taataacaac agcgacaagc ccgtgatgac atctggcatt
    43501 accagcggcg cggtgatcat aaaggcaaag ccatttgatc cgcgaaacct gccaaagcgc
    43561 accagcacca ccgccgcaat agtcccgagg atcgccgccg ccgttgccgc acaggcagca
    43621 attgtcaggc ttaaaccaac cgcactcatc atcgcgtcat cgcgcaataa ctcaccatac
    43681 cagcgcgttg accagccggc ccacaccgtc accagcttcg agctgttaaa cgaatagata
    43741 accagcatca gcattggcgc gtagagaaag gtaaagccca gcagcaaaat cacaatccgc
    43801 cagggcgaac gaactaccgg taaattattc atccgtgttc tcccacgctt ttttgctggt
    43861 gtttgtggaa ccacattatc ggcacaatta gcagcagcaa catgatgatc gctaccgccg
    43921 cggccaccgg ccagtcgcgg ttattgaaaa actcttgcca tagcacgcgc ccgatcatga
    43981 tgctgtccgg gccaccgagc agttccggga tcacaaactc gcccacagcc gggataaaca
    44041 ccagcatcga tccggcaata atcccacctt tagtcagcgg cacgatcaca gtaaagaacg
    44101 ttttcagcgg tcgtgcaccg agatccagcg ctgcttccac tagcgaataa tcaatacgaa
    44161 tcaacgcggt ataaatcggc agcaccataa acggcacgta ggcgtaaaca atgccaatat
    44221 aaactgccag attggtatga agaatggtca gcggttgatc gataaccccc agccacagca
    44281 gaaaattatt cagcacaccg ttgtttttta atattcccat ccaggcataa acgcggatca
    44341 gaaacgaggt ccacgacggc aggatcacca gtagtaataa aatattacgg gtcgaaggct
    44401 tactgtgcgc caccgcccac gccagcggat agccgatcag taaacagcaa aatgtctaaa
    44461 tcgcagccac ctgtagcgac tggagataag catcgaaata gagcggatcg tcggtcagtt
    44521 gcagaaaatt accgagatta agagtgatgg acagttgccc gtcagcccac tccatcagtt
    44581 cggtataagg tggaatagcg cgcgccatct ccgccaggct tattttaaag acaatcagaa
    44641 atggcagcag aaacagcaag atcaaccaga tatatggcaa cgcaatgacc agtttgcgcc
    44701 catgcttcat ttgcagctgc gacaaccaca gcttaaatcc gcccggcggt ttcgactggg
    44761 cagcaggttc aagtgtattc atctttgctc cttaaaccgt cagcaccaca cagctgtcca
    44821 cttcccagca caaacgcact tcgtcgcccc aggtcggtaa ccctttacga tgacgatggg
    44881 cgttttgtag ctgggcgcta atcatctgcc cacttttcag acgaacgtga tacaccgaaa
    44941 gatcgccgag ataggcaatg tgtatcacct cccccaccgc gaagttacaa ccattggcgg
    45001 gcggctcttc gcaaagcatg attttttccg ggcgcagcgc cacatgtacc ggcacgttat
    45061 cgaccaccga ggcatccgcg tcgactttca gtggatgcac cagcccaggc gaataaagca
    45121 caagcccatc ctcatgacgc tctttgagta caccttcaaa gacattaacc gaaccgataa
    45181 attcagcact gtagcgggtg gttggatgtt cgtagatctc ttctggctcg ccaatctgga
    45241 caaatttccc gcgattcata atggcgatac gtcccgccat cgtcatcgcc tcttcctgat
    45301 cgtgagtgac catcacacag gtcacgccga cacgctccag aatatccacc acttcaagct
    45361 gcatccggtc acgcagcttt ttatccagcg cgcccatcgg ctcgtcgagc agtaatagtt
    45421 tcgggcgctt ggcaaggctt cgggccagcg ccacacgctg tcgctgacca ccggaaagct
    45481 gatgcggttt gcgtttggcg aactcctgca tgtgcaccag gccgagcatc tcatggaccc
    45541 gactggcaat ttccgctttc ggtagtttgt cctgtttcag gccaaaagcg atgttctgtt
    45601 ccacggtcat atgcggaaac agcgcgtaag actgaaacat catattgatg gggcgcaggt
    45661 aaggcggcac ctgtgacaaa tcgacgccat cgagcattat ctgcccggca gaaggttgtt
    45721 caaaacctgc cagcatacgc agcagcgtgg acttgccaca gccggaagcg cccagcagcg
    45781 cgaagatttc acctttgtag atggtcaggc tgacatcatc gaccgcatgc tgaccatcgt
    45841 aggatttggt gaggttgcgg atttctaata gcggcgtcag cgccttacgg gttttcgcct
    45901 gcgggcgagg gatagcgtca ttcacggctg ctctccggca ttgaatgtgt gcaagcgcac
    45961 catgatggtg cgaattgccg gcgggtgtgg tgcgcccgcc cggcatctaa ggctgcggat
    46021 tattttccgc tcttcacttt ggtccacgcg cgggtgcgca cacggtcgat tttcggatcc
    46081 tgtactttca gagtgaatag cttcgcacga acgtccgccg gaggataaat acccgggttc
    46141 tcacggactt ccgcactcac cagcggcgtg gctgctttat tggcgttggc atagaacaca
    46201 tggtcggaaa tatgcgctac tacatccggg cgcagcaggt aattcaggaa ctgataggct
    46261 tcgtctttgt ttttggcatc cgcaggcatg gcgaatacat caaagaacgc catcgcccct
    46321 tcttttggaa tcgagaacga gacattcact ccattcttcg cttccttcgc gcggtttgac
    46381 gcctgccaga catcacctgc ccagccgata gcgacgcaaa tatcgccgtt tgccaggtcg
    46441 ttaatgtatt gagatgaatg gaaataacga atgttcgggc gcagctttaa cagcagatcg
    46501 gttgccggtc cggtgtaatc atccgcttta gtgctgttgg gatctttgcc gagataattc
    46561 aacacggtag caaaaacttc ttctggcgca tccaggaaag agacaccgca gcttttcagt
    46621 ttttccagat tttcaggttt gaggatcaag tcccagctat cgacgggcgc gttttcgccc
    46681 agcacagctt taactttatc aacgttatag ccaatcccgg tcgtcgccca catatagggc
    46741 atagcaaatt tattgtcggg atcgtgtttg gcgaccagct tcagcagctc cgggtcgaga
    46801 ttcttccact ccggcaattt gcttttgtcc agcggctgga aaactcccgc agtcaactgg
    46861 cgttcaagaa agctggcaga tggaaccacc aagtcaaagc cggtgctacc cgccattaat
    46921 ttgccttcca gtacttcgtt agagtcgaaa acatcgtaga cgactttaat accggtttct
    46981 ttttcaaaat tggccaccgt gtcaggggcg atataatcag accagttata aatgtggagt
    47041 gttttttgtt cagccgcgag cgtgccgaca gagacggcca tcagagcacc cgcaaccaga
    47101 cccgaaagcc attttttatt taaggcggtc atatctcttt ccttctgaaa gttcgttaac
    47161 aaacgaacaa aagttgtcat actaaaagat atgcaataaa cgtgcatatt ttttatcggc
    47221 gcggtgtaag aaaagagaac tctctcccgg ccagcactgc tcgctatata aaagcatcgc
    47281 aagaataact ttagccaggg tttgagacta tagctcggat taaaaaacgg tatttaacaa
    47341 attgttatgc gtatttgctc tatgtgataa agccaaatcg gcacaagaaa agtgaaataa
    47401 tctttaagaa aaggttaaaa acagcagata attttgcagt gcgcagtcac tgcaccagcg
    47461 actgcgtgct cgaaaggagt tatcagtgga ggaaatagtt ttcggtaacg ttgttttgcc
    47521 cttcatcgtc agtcggtaac agcagttgat gggcattagc ttcaagaatg accatcgaaa
    47581 tctgctcttc gctctgacgc acgaaccatg caaactgctc gtaggttacg ccctgcataa
    47641 cggataaaga ctggcaaacc accagctttg gcagattatc atcctgcata tcaagaaatg
    47701 ctttcacggt cagcgaactg gcattgatgg cagataaatc cgccgccagc ggcaatacgg
    47761 ctgaaggtcg gacttccgcc atggcagaaa acaggatcgt gttatcgatc agatcgattt
    47821 tggcatcaaa gacaccgtcg aaattctgca tatggggcag atgcagagcc tgacagttat
    47881 cacattcaaa aaaactcatc cccaggtcat cgagccattg acgcagcgta tccagaccag
    47941 gaacgaccag cgatgtcata attttgtaca acctctttcg ataaaaagac cggcacagct
    48001 tacgcaaaaa gcgcaggcaa aaccatgatc agtaatgtga ttgcgactaa ccacccgttt
    48061 tcaggcaata ttctgtcgta gcgtagcgtt cgatccagcg gatcatttta cccgcgatgt
    48121 cgataccagt ggttttttct attccttcca gccccggcga cgcattcact tccatcacca
    48181 acggcccgcg attagcacgc agaatatcca caccagcaac gtccagcgcc atcgttcgcg
    48241 cggcttttat cgcgatttca cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc
    48301 cacgatgcaa attggaacga aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca
    48361 cttcatcgcc aacaaccaga cagcggatat cgcacccttg cgcctctttg atatattcct
    48421 gcaccagaat atgcgcgttc agaccgcgga aagcgtcaat cacgctttcc gccgcctgac
    48481 gcgtcagcag ttgcatggaa cgcaatttgt cacgcgcccg ggcaatggcg accgactcat
    48541 tgagcggata gctccccagc atctcgaact ggcgcagtgc cgccgtccca taaaaggtaa
    48601 tggcggtgcc aatacgcggg atcactgcgt caaaatgggg taacttgcgg cctttgtagt
    48661 gaatagaaga cgccgcagga tttatgttca tgtagcaaga aagcggatca agaatttcaa
    48721 ccaggtgacc gcgctgtata gcggcttcac gcagccgctt acacgaatag agcgttccat
    48781 cccgggacaa tatggcaatt ttcaccctgc acctctctgt cagaccaggt caaaagcccg
    48841 cgtatcatac atcgacgtgg cggttttagc gcgtcgccca accctgtttg tgcaaataat
    48901 ccagaataaa tgggcggctt tctttaatga ttgttcggcg gatatgatcg ctccaggtat
    48961 cccggcgatt attgctgcca cgggtgaggt aatattccgc cagttgctcg tcatactgcg
    49021 ccagtgcgtc tttatccagc ggttgatagc tgttttcatg caccaaaatg gaggccggta
    49081 aacgcggctt gagatccgga ttatccgcag gccagccaag gcacagccca aacagcggca
    49141 gaacatgctg cggtaatttc agcagtttcg tcaccgcttc aatattattg cgcaggccgc
    49201 cgatatatac cccgcccaat cccagcgatt ccgctgcggt taatgcattc tgcgccatca
    49261 ttgccgtatc aacgacaccg agcaacagtt gttccgccag gccgagctga gcatccggac
    49321 agatctgtaa atggcggtta aagtcggcac agaacaccca gaactccgcc gcttgcgcta
    49381 cgtgtttttg cccgccggtc agcgtcacca gttcttcacg taatgctttg tcggtaatgc
    49441 gaataatgct actacactgc aaaaaactgg aactggacgt cgcacgggcg ctgttaataa
    49501 tcgcctcaca ctgcgcttcg gaaatgggtt catcagtgaa atggcgaatg gagcgatggc
    49561 caccaataag ttcaatggtt ggcgtcatta tctttttctc tttctgaacg tgaatattgc
    49621 ggtggacggt tcatcagctg tggggcaaga cgttttgcca cctgaagaat aaccaccacc
    49681 gcagcgggaa gcatgagcaa aacaccgaga aaaatcatca gaatctgcac ttctggccga
    49741 gaaaatggct caggcagcga cagggagtcg cttaccgaca gcagcgccac cgccagtagc
    49801 atcattccga taaattccag tatcaacacg cctttaggca atttaccgat cgcgcgcata
    49861 cgcttccctc tgcaaagtga gccttcagtc taaaactttt cactgtattg tgtttaacag
    49921 ttatagcttt tagcaattaa tgcaacaggt taaacctact ttcagcgaat acattttagc
    49981 gtgatcatta caggcataaa tctatgagga gagaaataat gcaaaccgtt atttttggtc
    50041 gttcgggttg cccttactgt gtgcgtgcaa aagatctggc tgagaaattg agcaatgaac
    50101 gcgatgattt tcagtatcag tatgtagata ttcgtgcgga agggatcact aaagaagatc
    50161 taccacaaaa ggcaggtaaa cccgtagaaa ccgtgccgca gatttttgtc gatcagcaac
    50221 atatcggcgg ctataccgat tttgctgcat gggtgaaaga aaatctggac gcctgatcgt
    50281 ctgacaagcc ctcgcgttga gggctttact gattttttct gtgctgtggt ttaaacaaac
    50341 tactgataaa taagaaacac agtgccccca gcgcacacca gaacaccgcg cttagtaacc
    50401 atgccagctc ttgccagaat gagcgcgtcg gtgaaaaaaa cagccgcata atgagcattg
    50461 aacagggtgc cgccagcatt gcgccaaata gaggtttcag gacttctcta cgctgtgaaa
    50521 agaaactggc aactgctcca ggaagaatga aaaacagcaa gccgatttca ggatgcccgg
    50581 cagcccgaaa agcgcctttc atgtacgtcg ccagaaaaag gcacaccaca atgaagagga
    50641 caaaacagca gattgcccct gcccaacgtt gtttatgttt cactcgttcc tcctgacact
    50701 gcgtctatcg aacacatttt tcgccagtgt ggcgttcagt aagataaagc cgcttcgcat
    50761 tccatgctaa tataggccaa cgcaattcat atagccgttg atacctaatg tgattacact
    50821 agtaaaatac attgttactt tactatcgtt taggtgcgct gaatgaatct gcgccctgaa
    50881 ttctggtaaa aaacattatc gtaaattacc atttctttca acagcttact agtaaacaag
    50941 aagttagcct ccgtgaatat aaacgtcgcc gaattgttaa atgggaatta cattctgtta
    51001 ttatttgtgg tcctcgcgct tgggctatgt ctcggaaagt tacgacttgg ttcgatccaa
    51061 ctgggtaatt ccattggcgt tttagtcgta tcgctgttat taggccaaca acatttcagc
    51121 attaacaccg atgcgcttaa tcttggcttt atgctgttta ttttctgcgt cggggtcgaa
    51181 gccggaccga actttttttc catttttttt cgcgatggga aaaattacct aatgttagca
    51241 ctggtgatgg ttggcagtgc gctggtgatc gccttagggt taggtaagct gtttggctgg
    51301 gatattggcc tgacggccgg tatgttagca ggctctatga cgtcgacacc ggttctggtc
    51361 ggtgctggcg atacactgcg tcattccggc atggaaagca ggcagctctc actggcactg
    51421 gataatctga gcctcgggta tgccttaacc tatttaatcg gtctggtgag tttgattgtt
    51481 ggtgcgcgtt acttgccgaa attgcagcat caggacttac agaccagcgc ccagcaaatc
    51541 gcccgcgaac gtggcctgga cactgatgcc aaccgtaagg tttatttacc ggtgatccgc
    51601 gcctatcgcg tcggcccgga gctggtggcc tggaccgacg gcaaaaatct gcgtgaactg
    51661 ggtatttatc gacaaaccgg ctgctacatt gaacgtattc gacgtaacgg gattctggca
    51721 aatccagacg gtgatgccgt gctacaaatg ggcgatgaaa tagcgttggt aggctatccc
    51781 gacgcccatg cccgactcga tcccagcttc cgtaacggta aagaagtttt cgatcgtgac
    51841 cttctcgaca tgcgtatcgt cactgaagaa gtggtcgtta aaaaccataa cgctgtaggt
    51901 aaacgtctcg cacaactgaa gttgaccgat cacggttgct tccttaaccg cgtcattcgt
    51961 agccagattg agatgccgat agatgacaac gtcgtgctta acaaaggtga cgttttacaa
    52021 gtcagcggtg atgcccgtcg cgtaaaaacc attgccgatc gcatcggctt tatctcgatt
    52081 cacagccagg tcactgacct gctggcattt tgcgccttct ttgttattgg gctgatgatc
    52141 gggatgatca ccttccagtt cagcacattc agtttcggca tggggaacgc tgccgggttg
    52201 ttattcgccg gaattatgct gggctttatg cgtgctaacc acccgacctt cggttacatt
    52261 ccgcaaggtg cattaagcat ggtgaaagag ttcggcttga tggtgtttat ggcaggcgtt
    52321 ggtctgagcg ccggtagcgg tattaataac ggcctggccg cgattggcgg tcagatgttg
    52381 attgccggat taattgtcag tcttgtgccc gtggttatct gtttcttgtt cggtgcttat
    52441 gtattgcgaa tgaaccgcgc actgttgttc ggcgcaatga tgggcgcacg cacctgcgcg
    52501 ccggcaatgg agatcatcag tgatacagct cgcagtaaca tccctgcgct gggctatgcg
    52561 ggcacctacg caatcgccaa cgtcctgctg acgctggcag ggacaatcat cgtcatggta
    52621 tggccaggat taggataaaa ctgaagttgc cctgaaaatg aaattttttt gcacaaccgc
    52681 agaacttttc cgcagggcat cagtcttaat tagtgccact gcttttcttt gatgtcccca
    52741 ttttgtggag cccatcaacc ccgccatttc ggttcaaggt tgatgggttt tttgttgtct
    52801 gaaatttatg ccttttaaaa tcatgatgtt agaagcactg ttttttaacg atggcgacaa
    52861 aatggcggca gcgtcaaaga gagagcgcca cctgtcctga tttcattgga tgcggctgaa
    52921 ccggatttga ctcttttggc gttgcaatcg aacgaacaaa agtttcatgg gtaacaaaag
    52981 tatggctgca gttaatgttc tggcactggt tgtaacgctc tttggtcaat gaagatacct
    53041 gaaaactgct gcgagtatgg gcggcacttc cacacagtgg gcaaatcatc atttttcgag
    53101 ttctccccat ttttgctaaa ttcacaataa tgataccgca ttattccatt ttgcaaactt
    53161 aaaagttctc cattgcgaag aatcattcca tttcgaaatc atcaatcctc acttcaagct
    53221 ccagactggt cgtaaaaccg ttatccgggc tgacagtatg cgtcagagtc gtaatggtcc
    53281 attccgcatc atctatcggc tgtttaaagc cactgacctt cacaggcatt tctgtgtaga
    53341 gatctgcccg cccttccgcc agttgtagcg agaatgatgc aacgccgcgt tgcaggcgtt
    53401 cccactgcat tttcgctgcc cgttcggcgt tgctccggtt ggcataagtg cgattaagta
    53461 ccagcacgtt ttcatccgta cccaccaggt aatcgccctg cttcgcttcc ggctctttct
    53521 tctgcttctt agtcctgcgc ttacgcttca cagtggtgct ttctttcttc gcgggttcgc
    53581 gggtatgcaa ccagctggca atgacgcccg tgtaagctcc gcgatctgcc agggtaaagc
    53641 ggtgactgtc gccgtccttg cgtgtgatag tgatcaccgg tagtggttta ccgctggcgc
    53701 ttttaccctg cccctgccgg atgaataaca gattgccatt tttcaccgac gcaatagcac
    53761 cgtactggcg cgccagccgc atcaaaaaac tgccgtcact ctcattagtc tggtctatat
    53821 gctccacgag tttatccgac aagtcttcac ccaatgccat cttcagtttg tgccgcgcag
    53881 ctatttcctt caccacttcc ccaacggtgg tcttgtgcca cgacttttca cggcgggtat
    53941 tcagcgtttc acgaaaatca gcacttcgcg cccggatagt caggcgatcc ggtgcgccag
    54001 tgtgttcaat ctcgtccacc gtgaatgccc ctttcgggaa aagcggctgt cccttccagc
    54061 ccagcgccag cgtaatgaca gccccacggc gcggcagcac gatttttcca tcggcgtcgt
    54121 ccagctccag atcaagctgg tccgcctcaa agccccggtt atccgtcagc gtcagcccca
    54181 tcaggcggtt gtccagcaca gtggtgatat ccctgccttc aatactgatg ctgaatgccg
    54241 gagttttgtt gcctttgtta agcagttcag agctgaaatt cacaacagca gccctcccac
    54301 cgttttactg atatcgctta aggcagatgt tgccgttccc tgcagattat tcagttgcgc
    54361 actgagatca ccgaacatat cggacaggga ttcatccacg cgttttagcg acagggtgaa
    54421 ctcaatccgg cgcggcatac cgtcgcggaa aaactccgtt ttagtctgat tcagtccctc
    54481 aatcacatac atgccgtaaa tcgtgccgct gccttcaatc aggggccatg cttttccctg
    54541 ttctgccatc tgctccagag ccagcaacga cagcctgccg cccgttacct ccggcataag
    54601 aacaccagaa agcgtcagca tgtcgttgtc cggtcccaga aactgcgttg acgggcgtcg
    54661 gtttacccgg ctgtttgccg catgtcgcca gctgcgttga tactgcagct cctgatacgg
    54721 cacggtgcgc agcataaaca cgtacaatcc cagcaccatc atcatgcgtc gtatcccccc
    54781 tgatcgctgt agttactcct ggcttttgcc ttcagcctgc gttcacgttc atcaagctgg
    54841 cgggccacct cccgcgcaat atcctgcgca ctttgtcctg gctgcgtttg aatgatgatc
    54901 tgcatcggtg cctcaatccg ttgaacgggc ggcacagtgg ctgcacgact caccatcgct
    54961 tcaccgcctt tcgcgggaag tgccaaaggg tgcaacggtg gaagctctgc tggcgtagca
    55021 gcaacaccca tcattccggc aacaacggca gccagtgcag ctgtatttct ccggctggtc
    55081 acatttgccg ggccgttaac aatttccggc ccgttttcac cgacgatgcc aaactgcccg
    55141 cgcgggatat acccgccgct gtcatacatc cccgcaaagc catatcccca tgacggaaaa
    55201 ccacccgatg gcatcatcac tttaccgtct gcattcaccg tcgcaggttg ctgacgcgtc
    55261 acgctttccg gcagtttcgc ctttgcggcc tctttactga caatgccaag tttctccagc
    55321 aaccaggaaa cgccggattt cagggagtcc agcggatgca tgaccatatt cagcccttcc
    55381 gccagagcct ccccgaatcg tcgccccatt gccgctgcac tctgcagttc ggcagaggtc
    55441 gacttaacgg gcgtcagcag atcagtaaac cagccccaca gcgcctgtac tttgtcgcca
    55501 atccactgga acacaggctt aagcggttcg aacgcggcac tgacaggacc tgccgccgct
    55561 ttgaatcctt ccaccacgcc accgagaaat gtggtgatag gctgccagta tttccagaca
    55621 accagcgcca cacccgccag tgcagtaacc acaagaccta tcagactgag cagagcacct
    55681 aacagaccag agacggcata cagggcaacg cgcagcatcg ccagtggacc ggatgcgagc
    55741 atacgcagca ccgtgcctgt ggcagccagt ccgccgcgta gtaccgccag tggattcata
    55801 aacatcacag caaccgcacg taaaccggat aatccagacc cgcaaaagtg caaccggcgc
    55861 acctgctaca gttttcagga catttcccgt cagtgatgcc gtgcggcgca aagacgacaa
    55921 cggcgcagta agtaaacctg cagcgttgcc cgatgaagca agcccgcgtc gcagcagtgc
    55981 cagtggtgcg ccagccagcc aggacaacgc gttgctggtt cgggttactg ctgccgtaac
    56041 ggaaggtaac gttttgatac ccagcacaga gaatcccaga cggatgactg ccagcggccc
    56101 cagcactgca gccagcgcca cctctaaggt gccgaggccc acggtaacgg cagctaccac
    56161 agcggctact ttcatcagag tgcccgtcag ttccgggtta gcttccaccc agcgacgcaa
    56221 cgcccccgtg acgcttttca ccgtgtacag aatatccatc agcggctggc gcagcgtttc
    56281 ccccaggctg ctgaaggtgt tctgtgctcc ggttttgacc agcaaccact gagcagaaag
    56341 tgaatccttg ttgatgtcgg attctttctg catggaaccg agcgcatcat tgcccgcagt
    56401 cagttttaac tggcgctgaa gttccggcag gttgtttgcc agtttcgctg cgtcatcgcc
    56461 aaactcttta ccaaacaaca tggtcatggc agacaggcgc ttatcctgtg gcagcgcatt
    56521 caccttctcc agcacccgct ggatagttcc catcgcatcc ttcgtcatct gcttttcaat
    56581 cacttctgga ttgagtttca gcagattcat cccttcaaag aaactcttgc tttgcatggt
    56641 ggcaatggac aattcacgca ccatcgcgtt tgctgcactg gctgcaacct caggcgcagc
    56701 gccgagtgtc aggaaggtgg aacccagcgc cgccgcttta cgataatcca gacggtcagc
    56761 cacaccgccc aggcgttgca tgacatcaat gatgtccgcc cctttcgaca tggcgttatc
    56821 atccagatag ttcagcgcat caccgagctg ctcaatattg cgggtaggga ttttgtagag
    56881 ctgggcgatt ttccccagac tttctgacag ttcatccgct ggcagttcaa aggctgttgc
    56941 cgcctttgcc gccgtgctgg cgaaggccag caggtcacgt ttctggtctt cccagctgtc
    57001 gtcagggttt gcgacgttca tgcgcgcacc accttcaacc agtgcggcga agtccacagc
    57061 accgttttcc atcggcaact gttcgctggc agccttgatg gcaccctgca tttcgtaaaa
    57121 acgcgcagtg cggttgccat tatcgtcacg cagaccattg acctgctttg ccacaccttt
    57181 catggcatct tccatgctgg tatagctttt tactgccgcc atcactggtg cgcccattgc
    57241 cagccctgca gccgtggtgg tggctccggc acctgcaata cgatccctta cctcaagacg
    57301 cctggcgtaa gccccccggg cggcgtgcat ttttcgctgt tgctccccga cacgtcgtaa
    57361 cctcgcttcc tgctcagaaa gctgcctgtt ataacgcatc gtttcacggg taatgcgggc
    57421 cgtcgcactg gcgccatcat tagctgaaat accagcacga taaagttctg cacgcacaag
    57481 cgccgtctgc tgctgcagct ttttctggcg ttcttccagg cgctgaacag ccagccgttg
    57541 acggcccaga gcaacaacct gacgttgcga aggcggcccc atcgctccca gttcctgact
    57601 gagcaaattt gcacgctggc gggcatagtt cagcctgtcg cctaatttct gattttctgc
    57661 ctgcagcttt cggaagctgt ccagactgct cccggcctga tcaagctgct ttattgcatc
    57721 gcgggatttt ttgacagcag cagccagttc tcttgaactg gcctgcgcgg atcgaaatgg
    57781 gcgggtgagt ttgtcaaccg cattaagaat cacctgcaga cgcaggttat tgtcactcat
    57841 ggctggcccc gcttctctga atcgctttat cccgccattc cagcacttcg gtcagcggca
    57901 taacgtcagt aacggatggc ggccagtgaa agatggtggc gatatcagcc accagatcgt
    57961 caaccgtcag gctgtcggta aaccggcaag caccgacttc ttcaacaaaa aagtgacaac
    58021 ctcaaccgac atggcagtga gatctgccgg gtccatctct gcaatttcct gtgcagtcag
    58081 tgccggactg gagatgcggg ggatcacggt catcatcgcg ttcacatcca tatccataat
    58141 ggcctgcagg cgtgtgccgc gcagcgcacc ggactgcggt ttacgcaaca caatttcggt
    58201 aatttctgtt ttaccgcgca tgatgggagt atccagttga atggtctttt cagtctgctt
    58261 atcgctcatt ttgttgtcct gtaaattagg ttctggcgcg gcattccgcg ccgttcagat
    58321 acatcagagg ccgagggcgt tgcggtgcgc ttccatcagg tccacaccgt ccacaatttc
    58381 caccatgttg atcaggtcca cttcatagag cacctcacca ttgatggtca gcttcgcgta
    58441 gctgttggta ctggtcactt tggtagtgtt gctttcgccc gtcttccact cgccggaatc
    58501 tacttctttg tgacgtccac gcaccacaag ctccacggcc tgcacttccc cggtatcatc
    58561 acgctgaata gagccggtaa agcgcagctg gatgccatcc accgtggctt tgcccatctg
    58621 cttaaacagc agcagttcag taccaccaat ggaaaattct gtatccagtg cactgtcatc
    58681 cagtcccaga tccacatcca ccgctccggg cataccgccg ccgcgatact tctcatattt
    58741 gcgggtgaat ttcggcagcg tcagcgactc aacgatcccc tgccagttgt tcccgtcgtt
    58801 aaacaggttc aggtgtttta atttgcgtgg taaagccatg ttgtcccctt acgcgctgac
    58861 ctggctggag aaattcacca ggtactgatc ggtgatgcgc tgacgcagca tcaggttttc
    58921 aagtggcggc actggcgtgt agtcgtaatc gatggtgagt ttcccggctt tcagcgtgtc
    58981 tttatcgttc accgactcgt ccagccagca atcaccacca atgagatagc cctgactgac
    59041 caggctacgc attttggcgc ggataccttc gataatgtcg cgggccagcg acgggttcag
    59101 cggtttgtcc accgcccaca tgtgcgcttc tgccatcgtg tccatcagca cctgcgccgt
    59161 gcgggtgtag ttttcgaagg caaagagcgg atcatcgctc agacagcggg aaccccagaa
    59221 gcggaaaccg tctttgcgga taagcgtggt gatatcgttc tggttaagca gacctgcatc
    59281 ggttgccggg ttctgcagat cccagaacac atcagcagaa attccggtga caccgttcac
    59341 gcccacgttg gacaggcttt tgtgccatcc ggtctgctca tcaattttag cgcgcagacc
    59401 gagcgcacgg gcggtggcat aagccgttgc ttcggcattc agcaccgtgt cccagccagt
    59461 aaagtcgggc cagatcagca ttccttcacg ctgactgaag ttttcacggt aagtgattgc
    59521 ttcctgcacc gtcttgcaac catacgctga caggtaagca aatccacgca ggctttgcgc
    59581 cacgctcagc aactcagtag ctaccgcctt cgtgtcgtga cctggcacgc cgagaatgcg
    59641 cggtttaacg ccgagctgtg actgggcaga taacagggct ttcatacctg tttttttacc
    59701 ctcggcggtc actgcgccaa tgatattggt tgtggtttct tcttccgttt caccctgcgg
    59761 cacacgcaca acaacggtca cgggttttgc ctggtcagcg atggcatcca gcgaacgggc
    59821 cagcgtgcca gactcacccg ctttaccgct ggcggtcagc acatcagtga tcagcacagg
    59881 tttattaaga ggaaacattt ttgcatcggc atcatcgccc gtgcagacca tacccacgat
    59941 ggcggtgctc accgtggtaa tggatcgggt gccttcgttg acttcaacaa cgcgcacccc
    60001 gtggtggtaa tcctgagcca tagtggcgaa cctcctgatt ggattaggct tcgccctatg
    60061 ttgaagtgat tgtgcctgac aaacagctaa gcgcagttgt gtcgttattc acacaaaatg
    60121 acggtatttg tccgcttaca ggaaaaatca aaataatact gactcagggc gattcattgc
    60181 tctcattcgc cggaaatttt ctataaatgg tagaaacgcc cacatcaaaa atcagtgcaa
    60241 tacgctgtct tgattctccg gcctcgagta aacgtccaat ctgtgcccac tgttcggtgg
    60301 tcaacttagg acggagtcca cctactctgc ctttggcacg agctgcagcc agccctgctc
    60361 tggtacgttc aactatcagt tcacgttcca tttcagccag agcccccatg acgtgaaaaa
    60421 agaaacggcc cattggggta ctggtatcaa tactgtcagt caggcttcgg aaattcacac
    60481 cacgctggcg caactcttct atcagcgtaa caagatgccg catactgcgt cccagtctgt
    60541 ccagcttcca gacaaccagc gtgtctcctg ccgatagtgt cctgagcagt tttttaagcc
    60601 ccggtctgtc gggcttagtg ccactgattt tatcctcaaa aatccgctca catcccgcgc
    60661 agttcagtgc attacgttgc aaatcggtgt tctggtcatt tgttgacacg cgtacatagc
    60721 caataagcat gatcaatccc ctgaataaaa accggggatg atgccagtta gccattatct
    60781 ctgcattttc ttaaacgttg gtttgggaga aggctctgca ttacctgttg gtgtgcctgt
    60841 tccgtggcct tcagccactc cgccgacagg ctggctgaaa tgcaatggtg cggctttttc
    60901 tgctgaagaa tacccggaac tggcaaaggc ttacccgaca aataaattgc ctgatttacg
    60961 cggtgagttt attcgtggct gggatgacgg acgtggagtg gataacggaa ggggattatt
    61021 aacgcttcag gacggtgcga ttgtcagtca taaccactat tggggaatct ggacttcacg
    61081 aactaacgac cagactctgg aaagttttac aggcaccacg attttaaaac aaatcacgcc
    61141 cctgtctccg gccattgact tcgataatta cccaattccc aacccggcta ttacagaggg
    61201 tggtgttgtt gcggcaacga ctaaacctgc aggtgcgaat gaaacacgcc cacgaaatgt
    61261 cgcttttaac tatattgtga gggctgca
//
LOCUS       DAJTMK010000003        60057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTMK010000003 DAJTMK010000000
VERSION     DAJTMK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60057
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.003"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(2..94)
                     /locus_tag="OO135_000126"
     tRNA            complement(2..94)
                     /locus_tag="OO135_000126"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO135_000127"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO135_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5396115.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO135_000128"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO135_000128"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5396116.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKDVEIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO135_000129"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO135_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5396117.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO135_000130"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO135_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5396118.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARIMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO135_000131"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO135_000131"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5396119.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO135_000132"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO135_000132"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5396120.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVGFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8018
                     /gene="srlA"
                     /locus_tag="OO135_000133"
                     /gene_synonym="gutA"
     CDS             7455..8018
                     /gene="srlA"
                     /locus_tag="OO135_000133"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5396121.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8015..8974
                     /gene="srlE"
                     /locus_tag="OO135_000134"
                     /gene_synonym="gutE"
     CDS             8015..8974
                     /gene="srlE"
                     /locus_tag="OO135_000134"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5396122.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8985..9356
                     /gene="srlB"
                     /locus_tag="OO135_000135"
                     /gene_synonym="gutB"
     CDS             8985..9356
                     /gene="srlB"
                     /locus_tag="OO135_000135"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5396123.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAVFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9360..10139
                     /gene="srlD"
                     /locus_tag="OO135_000136"
     CDS             9360..10139
                     /gene="srlD"
                     /locus_tag="OO135_000136"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5396124.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10244..10603
                     /gene="gutM"
                     /locus_tag="OO135_000137"
     CDS             10244..10603
                     /gene="gutM"
                     /locus_tag="OO135_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5396125.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10670..11443
                     /gene="srlR"
                     /locus_tag="OO135_000138"
                     /gene_synonym="gutR"
     CDS             10670..11443
                     /gene="srlR"
                     /locus_tag="OO135_000138"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5396126.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11436..12401
                     /gene="gutQ"
                     /locus_tag="OO135_000139"
     CDS             11436..12401
                     /gene="gutQ"
                     /locus_tag="OO135_000139"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5396127.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGSALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12398..13912)
                     /gene="norR"
                     /locus_tag="OO135_000140"
     CDS             complement(12398..13912)
                     /gene="norR"
                     /locus_tag="OO135_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5396128.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPSDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14099..15538
                     /gene="norV"
                     /locus_tag="OO135_000141"
     CDS             14099..15538
                     /gene="norV"
                     /locus_tag="OO135_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5396129.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECFLGKDVFEELASEAK"
     gene            15535..16668
                     /gene="norW"
                     /locus_tag="OO135_000142"
     CDS             15535..16668
                     /gene="norW"
                     /locus_tag="OO135_000142"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5396130.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDYAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPTMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="OO135_000143"
     CDS             complement(16796..19048)
                     /gene="hypF"
                     /locus_tag="OO135_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5396131.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGLRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEASLQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="OO135_000144"
     CDS             complement(19201..19728)
                     /gene="hydN"
                     /locus_tag="OO135_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5396132.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19877..20887)
                     /gene="ascG"
                     /locus_tag="OO135_000145"
     CDS             complement(19877..20887)
                     /gene="ascG"
                     /locus_tag="OO135_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5396133.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            21147..22604
                     /gene="ascF"
                     /locus_tag="OO135_000146"
                     /pseudo
     CDS             21147..22604
                     /gene="ascF"
                     /locus_tag="OO135_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
     gene            22613..24037
                     /gene="ascB"
                     /locus_tag="OO135_000147"
     CDS             22613..24037
                     /gene="ascB"
                     /locus_tag="OO135_000147"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5396134.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24191..24661)
                     /gene="hycI"
                     /locus_tag="OO135_000148"
     CDS             complement(24191..24661)
                     /gene="hycI"
                     /locus_tag="OO135_000148"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5396135.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(24654..25064)
                     /gene="hycH"
                     /locus_tag="OO135_000149"
     CDS             complement(24654..25064)
                     /gene="hycH"
                     /locus_tag="OO135_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5396136.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25061..25828)
                     /gene="hycG"
                     /locus_tag="OO135_000150"
     CDS             complement(25061..25828)
                     /gene="hycG"
                     /locus_tag="OO135_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5396137.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQQLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(25828..26370)
                     /gene="hycF"
                     /locus_tag="OO135_000151"
     CDS             complement(25828..26370)
                     /gene="hycF"
                     /locus_tag="OO135_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5396138.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(26380..28089)
                     /gene="hycE"
                     /locus_tag="OO135_000152"
     CDS             complement(26380..28089)
                     /gene="hycE"
                     /locus_tag="OO135_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5396139.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTGAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGSAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDYGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTHQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPVEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28107..29030)
                     /gene="hycD"
                     /locus_tag="OO135_000153"
     CDS             complement(28107..29030)
                     /gene="hycD"
                     /locus_tag="OO135_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5396140.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29033..30895)
                     /gene="hycC"
                     /locus_tag="OO135_000154"
     CDS             complement(29033..30895)
                     /gene="hycC"
                     /locus_tag="OO135_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5396141.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGL
                     VGGLYHLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMA
                     ALPPLNGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVT
                     FLGAPRTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANT
                     TVSQPMITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAM
                     PVKQAFAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(30892..31503)
                     /gene="hycB"
                     /locus_tag="OO135_000155"
     CDS             complement(30892..31503)
                     /gene="hycB"
                     /locus_tag="OO135_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5396142.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCQLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(31628..32089)
                     /gene="hycA"
                     /locus_tag="OO135_000156"
     CDS             complement(31628..32089)
                     /gene="hycA"
                     /locus_tag="OO135_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5396143.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32301..32651
                     /gene="hypA"
                     /locus_tag="OO135_000157"
     CDS             32301..32651
                     /gene="hypA"
                     /locus_tag="OO135_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5396144.1"
                     /translation="MHAITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            32655..33527
                     /gene="hypB"
                     /locus_tag="OO135_000158"
     CDS             32655..33527
                     /gene="hypB"
                     /locus_tag="OO135_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5396145.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            33518..33790
                     /gene="hypC"
                     /locus_tag="OO135_000159"
     CDS             33518..33790
                     /gene="hypC"
                     /locus_tag="OO135_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5396146.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            33790..34911
                     /gene="hypD"
                     /locus_tag="OO135_000160"
     CDS             33790..34911
                     /gene="hypD"
                     /locus_tag="OO135_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5396147.1"
                     /translation="MRFVDEYRTPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIEAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            34908..35918
                     /gene="hypE"
                     /locus_tag="OO135_000161"
     CDS             34908..35918
                     /gene="hypE"
                     /locus_tag="OO135_000161"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5396148.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            35992..38060
                     /gene="flhA"
                     /locus_tag="OO135_000162"
                     /pseudo
     CDS             35992..38060
                     /gene="flhA"
                     /locus_tag="OO135_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(38097..38450)
                     /locus_tag="OO135_000163"
     CDS             complement(38097..38450)
                     /locus_tag="OO135_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5396149.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKARE"
     gene            complement(38527..38661)
                     /locus_tag="OO135_000164"
     CDS             complement(38527..38661)
                     /locus_tag="OO135_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396150.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            38736..41297
                     /gene="mutS"
                     /locus_tag="OO135_000165"
     CDS             38736..41297
                     /gene="mutS"
                     /locus_tag="OO135_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5396151.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41403..42059
                     /gene="pphB"
                     /locus_tag="OO135_000166"
                     /pseudo
     CDS             41403..42059
                     /gene="pphB"
                     /locus_tag="OO135_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(42110..42877)
                     /locus_tag="OO135_000167"
     CDS             complement(42110..42877)
                     /locus_tag="OO135_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5396152.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            43073..43981
                     /locus_tag="OO135_000168"
     CDS             43073..43981
                     /locus_tag="OO135_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5396153.1"
                     /translation="MKTGSEFHVGIVSLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVVQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            43978..45240
                     /locus_tag="OO135_000169"
     CDS             43978..45240
                     /locus_tag="OO135_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5396154.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45237..45875
                     /locus_tag="OO135_000170"
     CDS             45237..45875
                     /locus_tag="OO135_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5396155.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEATNNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            45880..46656
                     /locus_tag="OO135_000171"
     CDS             45880..46656
                     /locus_tag="OO135_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5396156.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            46745..48109
                     /locus_tag="OO135_000172"
     CDS             46745..48109
                     /locus_tag="OO135_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR5396157.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAVFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAAI"
     gene            complement(48203..49195)
                     /gene="rpoS"
                     /locus_tag="OO135_000173"
     CDS             complement(48203..49195)
                     /gene="rpoS"
                     /locus_tag="OO135_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5396158.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(49258..50349)
                     /gene="nlpD"
                     /locus_tag="OO135_000174"
     CDS             complement(49258..50349)
                     /gene="nlpD"
                     /locus_tag="OO135_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5396159.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(50489..51115)
                     /gene="pcm"
                     /locus_tag="OO135_000175"
     CDS             complement(50489..51115)
                     /gene="pcm"
                     /locus_tag="OO135_000175"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5396160.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(51109..51870)
                     /gene="surE"
                     /locus_tag="OO135_000176"
     CDS             complement(51109..51870)
                     /gene="surE"
                     /locus_tag="OO135_000176"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5396161.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(51851..52900)
                     /gene="truD"
                     /locus_tag="OO135_000177"
     CDS             complement(51851..52900)
                     /gene="truD"
                     /locus_tag="OO135_000177"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5396162.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(52897..53376)
                     /gene="ispF"
                     /locus_tag="OO135_000178"
     CDS             complement(52897..53376)
                     /gene="ispF"
                     /locus_tag="OO135_000178"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5396163.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(53376..54086)
                     /gene="ispD"
                     /locus_tag="OO135_000179"
     CDS             complement(53376..54086)
                     /gene="ispD"
                     /locus_tag="OO135_000179"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5396164.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(54105..54416)
                     /gene="ftsB"
                     /locus_tag="OO135_000180"
     CDS             complement(54105..54416)
                     /gene="ftsB"
                     /locus_tag="OO135_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5396165.1"
                     /translation="MGKLTLLLLAILIWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(54610..54933)
                     /locus_tag="OO135_000181"
     CDS             complement(54610..54933)
                     /locus_tag="OO135_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5396166.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(54983..55588)
                     /gene="cysC"
                     /locus_tag="OO135_000182"
     CDS             complement(54983..55588)
                     /gene="cysC"
                     /locus_tag="OO135_000182"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5396167.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGVLEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(55588..57015)
                     /gene="cysN"
                     /locus_tag="OO135_000183"
     CDS             complement(55588..57015)
                     /gene="cysN"
                     /locus_tag="OO135_000183"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5396168.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(57017..57925)
                     /gene="cysD"
                     /locus_tag="OO135_000184"
     CDS             complement(57017..57925)
                     /gene="cysD"
                     /locus_tag="OO135_000184"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5396169.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWAP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            58177..59214
                     /gene="iap"
                     /locus_tag="OO135_000185"
     CDS             58177..59214
                     /gene="iap"
                     /locus_tag="OO135_000185"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5396170.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEVVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(59421..59714)
                     /gene="cas2"
                     /locus_tag="OO135_000186"
     CDS             complement(59421..59714)
                     /gene="cas2"
                     /locus_tag="OO135_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR5396171.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(59714..>60057)
                     /locus_tag="OO135_000187"
     CDS             complement(59714..>60057)
                     /locus_tag="OO135_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="subtype I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR5396172.1"
                     /translation="SLPAAGYAPAIGFIHSGKPLSFVYDIADIIKFDSVVPKAFEIAA
                     RQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGEIEPPQPAPDMLPPAIPEPE
                     TLGDSGHRGRG"
BASE COUNT        14202 a        16426 c        15503 g        13926 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt cacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggtgtttgat
     1981 ctagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcgtcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatttcg acgtcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactgattca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc tagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggacgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctacgcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatt atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 ccccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggtg tccggctggg
     5041 agcacagcag gttgtcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaagcaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcgcctatca ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttca
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgcaaa tagttactgc
     6121 acgcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttataac gggtaatggt gtagaagttc ggcagaccgt accagtactg gtaaccggta
     6241 ccgacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgccg tggcaagctg cgaaatgctg tacctggttt tgaagccatt tggcaagcct
     6361 ggagcctgac cgtttgccat taccgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaacccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac gccccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tccttttact caaactttca aattaaaata tttatctttc atttttagat caaaatatca
     7381 cttttaaatc tttcaatctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgctggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct gctgctaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctctttcccc
     7801 atatcaaccc tggcgaactg tttgtttatc ttggcattgc cagcggtctg acaacgctga
     7861 acctgccact tggcccactg gcggtgagct atctgctggt tggtctggtc accaacttct
     7921 ttcgcggctg ggtgaccgat ctgaccaccg ccatttttga gaaaaagatg ggcattcaac
     7981 ttgaacaaaa agttcacctg gcaggagcaa catcatgacg cgtattcgga tcgaaaaagg
     8041 aacgggtggc tggggcggcc cgcttgagct ggaagccacg ccgggcaaaa aaatcgtcta
     8101 tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg gcacaactta ctggctggca
     8161 ggctattgac ggatttaaag aaggtgaacc cgcggaggcg gaaattggtg tcgcggtaat
     8221 cgactgtggc ggcacattac gctgcggcat ctatccgaaa cgacgtattc ccaccattaa
     8281 tatccactcg acgggcaagt ccggtccgct ggcgcagtac attgtggaag atatttatgt
     8341 ctctggcgta aaagaagaaa acatcactgt agtgggtgat gcgacaccac aaccctcttc
     8401 cgtgggccgt gactatgaca ccagtaagaa aatcaccgaa caaagcgatg gtttactggc
     8461 gaaggtggga atgggcatgg ggtccgccgt tgcggtgctg tttcaatctg gtcgtgacac
     8521 catcgacact gtattaaaaa ccattctgcc gtttatggca ttcgtctcgg cgctcattgg
     8581 catcattatg gcttctggcc ttggtgactg gattgcccac ggtcttgctc cgctggcgag
     8641 ccatccactg ggtctggtca tgctggcgct catctgctcc ttcccactgc tttcaccttt
     8701 cctcggccca ggcgcagtta tcgcacaggt tatcggcgta ttgattggcg tgcagattgg
     8761 tctcggcaat attccgccgc atctggcttt accggcactg tttgccatca acgcgcaggc
     8821 ggcctgcgac ttcatcccgg tcggtttgtc gctggcggaa gcccgtcagg acacggttcg
     8881 cgtcggtgtc ccttctgtac tggtgagccg ctttttaacc ggcgcaccga ctgtactgat
     8941 cgcctggttt gtctccggtt ttatctatca atagaggctg aaacatgacc gttatttata
     9001 agaccaccat cacccgtatc ggcgcgagtg ccactgacgc cctcagcgac cagatgctca
     9061 tcacctttcg tgaaggcgcg ccagcggacc tcgaagagta ttgcttcatt cattgccacg
     9121 gcgagttgaa aggtgcactc catcccggtt tgcaatttac gctcgggcag catcgctatc
     9181 cggtgaccgc tgttggcagc gtggcggaag acaaccttcg cgaactgggt catgtcaccc
     9241 tgcgcttcga tggtttaaac gaagcggtat ttccgggcac tgtccatgtg gcaggccctg
     9301 tacccgacga tatcgcgccg ggatcggttt tgaagtttga atctgttaag gagtaaaaaa
     9361 tgaatcaggt tgccgttgtc atcggtggtg ggcaaacctt aggcgcgttc ctgtgccacg
     9421 gtctggttgc cgaggggtat cgcgtcgcgg ttgtcgatat tcagagcgac aaagccgcaa
     9481 atgtggcaca agaaattaac gccgaatatg gtgaaagtat ggcgtacggt tttggtgctg
     9541 acgccactag cgagcaaagc gttctggcgc tctctcgtgg ggtagatgaa atctttggtc
     9601 gcgtggattt gctggtctat agcgccggaa tagcaaaagc agcctttatc agcgacttcc
     9661 agctcggcga ttttgaccgt tcgctacaag tgaatctggt gggttatttc ctgtgtgccc
     9721 gtgaattttc gcgtttgatg atccgcgacg ggattcaggg tcgcattatt cagatcaact
     9781 cgaaatccgg caaagtgggc agcaaacaca actctggcta cagcgcagcg aaatttggtg
     9841 gcgtcgggct gactcaatca ctggcgctgg atctggcgga gtacggcatt acggtgcatt
     9901 cactgatgct cggtaacctg ctgaaatcgc cgatgttcca gtcactgttg ccacaatacg
     9961 cgaccaagct gggtatcaaa ccggatcaag tcgagcagta ttacatcgac aaagtaccgc
    10021 tcaaacgcgg ctgcgattat caagatgtgc tgaatatgct gctgttctac gccagtccta
    10081 aggcgtcgta ctgcaccgga cagtcgatca atgtcaccgg cggtcaggtg atgttctgat
    10141 caacagcgga gatccattaa ggatctccgt gagactatag aatgcctgat gcgctacgct
    10201 catcaggcat acaggacttc cgccactaca ttaaggaaaa gttatggtat ccgcactcat
    10261 caccgtcgcc gttatcgcct ggtgtgcgca actggcctta ggcggctggc aaatttctcg
    10321 ttttaaccgt gccttcgaca cactatgcca gcaagggcgg gttggcgtgg gccgttccag
    10381 cgggcgcttt aaaccgcggg tcgtggtcgc catcgcgctg gacgatcagc agcgcatcgt
    10441 cgacaccttg tttatgaaag gactgaccgt cttcgcccga ccgcaaaaaa ttcccgcaat
    10501 taccggtatg catgcgggtg atttacagcc cgatgtgatc tttccccatg atccactatc
    10561 acagaatgct ctatcattgg cgcttaaact gaaacgtgga taatttcgtt gtgaatgtta
    10621 cttgcttgcg aagttatcat tttgaaacct aaatcaggta atcacgccca tgaaacctcg
    10681 tcagcgtcag gccgccattc tggagtatct gcaaaagcag ggtaaatgct cggttgaaga
    10741 attggcgcaa tactttgaca ccacaggcac aaccattcgc aaagatctgg tcattctgga
    10801 acatgccgga accgtcattc gtacttatgg cggagtggtg ttgaataaag aggaatccga
    10861 tccgcctatc gatcataaaa cactcatcaa cacccacaag aaagagctga ttgcagaagc
    10921 tgccgttagt tttatccatg atggcgattc gatcattctt gatgctggca gtaccgtttt
    10981 gcagatggtt cccctgctct cgcgctttaa taacatcacg gtgatgacca acagcctgca
    11041 tatcgtcaat gcgctatccg aactggataa cgaacaaact atcctgatgc caggcggaac
    11101 gtttcgtaaa aaatcggcct catttcacgg ccagctggca gagaatgcct tcgaacattt
    11161 cagtttcgat aaattgttta tgggcaccga cggcatcgat ctcaatgcgg gcgtaaccac
    11221 cttcaacgag gtttataccg tcagtaaggc aatgtgcaat gccgcgcgcg aagtgatttt
    11281 gatggcggac tcatcaaagt ttggccgtaa aagccccaac gtagtttgca gtcttgaaag
    11341 cgtcgataag ctgattaccg acgcaggtat cgatccggcg tttcgtcagg cgctggaaga
    11401 gaaagggatc gatgtgatca taaccggaga gagcaatgag tgaagcacta ctgaacgcgg
    11461 gacgtcagac gttaatgctg gagttgcagg aagcaagccg tttaccggaa cgtctgggcg
    11521 atgattttgt tcgcgccgcc aatatcatcc tgcactgcga aggcaaagtg gtggtttcgg
    11581 gaattggcaa atcgggccac attggtaaga aaatcgccgc aacacttgcc agtaccggca
    11641 caccggcttt ttttgtccat ccggcagaag cactgcacgg cgatctgggg atgattgaaa
    11701 gccgcgatgt gatgctgttt atctcttact ccggtggcgc gaaggaactg gatctgatta
    11761 ttccgcgtct ggaagataaa tctatcgcgc tgctggcgat gaccggcaaa ccgacgtcac
    11821 cgctgggcct ggcggcaaaa gcggtgctgg atatctccgt agaacgcgaa gcctgcccga
    11881 tgcaccttgc gccgacctcc agcaccgtca ataccctgat gatgggtgac gcgctggcga
    11941 tggcggtcat gcaggcgcgc ggatttaatg aagaagattt tgcccgctcc cacccagccg
    12001 gggcactggg cgctcgcttg ctgaataaag tgcatcatct gatgcgccgt gacgatgcca
    12061 tcccacaggt ggcgttaacc gccagcgtga tggatgcgat gctggaactc agccgcaccg
    12121 gtctggggct ggtggcggta tgtgacgctc aacaacaggt acaaggcgtc tttaccgacg
    12181 gcgatttacg tcgctggctg gtaggcggca gcgcactcac cacgccagtt aatgaagcga
    12241 tgacgacagg cggcaccacg ttacaggcgc aaagtcgcgc catcgacgcc aaagagatcc
    12301 tgatgaagcg caaaatcact gccgcaccgg tggtggatga aaacggcaaa ctcaccggcg
    12361 caattaacct gcaggatttc tatcaggccg ggattattta atccttcatt cccagacgtt
    12421 tcgccagccg atgcaggttg gcgacgtcgg tttccagcat ccgcgcgcag gcagcccagt
    12481 tatgatgatt ttgtgccagt gcctggcgaa tagtttcacg ctggaacgct tctgtcgctt
    12541 cacgcaggtt ttgcttaaca acgggcaccg ccgccgcttc tggcggcggc aacgtcacct
    12601 caggaaaagc aaaatgttgc gcctcaagaa tcacttcatc gccgttgcgg gtggctctcg
    12661 ccagcactac cgcccgatga atagcatgtt ccagttcgcg cacgttcccc ggaaaacgat
    12721 agtgttgcag taaatttcgc gctccggcac ttaataccac gcgggagagc cccagccgca
    12781 aacgacactg ttcgcagaaa taccccgcca gcagaatgac atcatcgccc cgctcacgca
    12841 gcggcggcac cgaaagtgga aacacgctca ggcgatgaaa caagtcagcg cgaaatcgcc
    12901 ctgccagcac ctcttcgcgt aagtcgcggt tagtcgccgc cagcacgcgc acatcgaccc
    12961 gcaaactacg gtcatcgcca acgcgctgaa tatcgccata ctgcaacacc ctcagcagct
    13021 tggcctgcaa tgccaacgac aactcgccga tctcatccag aaacagagtg ccgttatccg
    13081 ccatttcaaa cttcccgctg cggttactga tagcgccagt aaacgctcct ttcacatgcc
    13141 cgaacaactc actttccgcc acactttccg gcagtgcagc acagttgaga tagaccagcg
    13201 gattcaccgc ccgtggcgag gcttcatgaa tcgctttcgc caccagctcc ttaccggttc
    13261 ccgtctcacc gctgatcagg acgttgagat cggacgccgc cacaatctca atctcttttt
    13321 tcagttgcgt catgccaggg gacaagccaa tcatctgcgt ctgtttcacc gcttcaaacg
    13381 gcgtggcatc gcttggcatc atattctggc tttccagttg ttcaatcagc aacgcattgc
    13441 ttaacgctcc cgccgccagc gcagcaatca gccgtagctc ttcgtcgctg aaaacatcga
    13501 actgatcggg ctgcatcccg tcgagcgtca gtgcgccgat caggttttgc ccggcaaaca
    13561 atggcagacc aacgcaggcg tgaaccttca gactctcctg cccaggaatc aaaccgtcat
    13621 agggatcggg caattcgctg tctgcgggaa agcgcaccac atccccggcg cgggcaatcg
    13681 cttccagccg tggatgccct tccagcgcaa agcgtctacc gagtacaccc tttgccagac
    13741 cgtcgatggc aagcggaata aactgccgcg aatcgtaacg tagcaacgca gacgcatcgc
    13801 actccagcac ctgacgtagc gtggtgatca ggcgctgaaa acgatcctga tgaccaatcc
    13861 cacgctgcaa ttcgatggcg atattcgcca gcacatcaac ggaaaaactc atctttgcct
    13921 cactgtcaat ttgactatag atattgtcat atcgaccatt tgattgatag tcattttgac
    13981 tactcattaa tgagcataat tttatttata gagtaaaaac aatcagataa aaaactggca
    14041 cgtaatctgc aattagcaag acatcttttt agaacacgct gaataaattg aggttgctat
    14101 gtctattgtg gtgaaaaata acattcattg ggttggtcaa cgtgactggg aagtgcgtga
    14161 ttttcacggt acggaatata aaacgctgcg cggcagcagt tacaacagct acctcatccg
    14221 cgaagaaaaa aacgtgctga tcgacaccgt cgaccataaa ttcagccgcg aatttgtgca
    14281 gaacctgcgt aatgaaatcg atctggcgga tatcgattac atcgtgatta accatgcaga
    14341 agaggaccac gctggggcgc tgaccgaact gatggcgcaa attcccgata cgccgattta
    14401 ctgtactgcc aacgctatcg actcgataaa tggtcatcac catcatccgg agtggaattt
    14461 taatgtggtg aaaactggtg acacgctgga tatcggcaac ggcaaacagc tcatttttgt
    14521 cgaaacgcca atgctgcact ggccggacag catgatgact tacctgacag gcgacgcggt
    14581 gctgttcagt aacgatgctt tcggtcagca ctactgcgac gagcatctgt tcaacgatga
    14641 agtggatcag acggagcttt tcgagcagtg ccagcgttac tacgccaata tcctgacgcc
    14701 gttcagccgc ctggtaacac cgaaaattac cgagatcctg ggctttaact taccagtcga
    14761 tatgatagcg acctctcacg gcgtggtatg gcgcgataac ccgacgcaaa ttgtcgagct
    14821 gtacctgaaa tgggcggctg attatcagga agacagaatc accattttct acgacaccat
    14881 gtcgaataac acccgcatga tggctgacgc tatcgcccag gggattgcgg aaaccgaccc
    14941 acgcgtggcg gtgaaaattt tcaacgtcgc ccgaagcgat aaaaacgaaa tcctgaccaa
    15001 tgtcttccgc tcaaaaggcg tgctggtcgg cacttcgacg atgaataacg tgatgatgcc
    15061 gaaaatcgcc gggctggtgg aggagatgac tggtttacgc ttccgtaaca aacgcgccag
    15121 tgctttcggc tctcacggct ggagcggcgg tgcggtggat cgtctttcca cgcgcctgca
    15181 ggatgcgggt ttcgaaatgt cgcttagcct gaaagcgaaa tggcgaccag accaggacgc
    15241 tctggagtta tgccgtgaac acggtcgcga aatcgcccgt cagtgggcgc tcgcgccgct
    15301 gccgcagagc acggtgaata aggtagtcaa agaagaaacc tctgccacca cgacggctga
    15361 cctcggccca cggatgcagt gcagcgtctg ccagtggatt tacgatccgg caaaaggcga
    15421 gccaatgcag gacgttgcgc caggaacgcc gtggagtgaa gtcccggata acttcctctg
    15481 cccggaatgc ttcctcggca aagacgtctt tgaagaactg gcatcggagg caaaatgagt
    15541 aacggcattg tgatcatcgg ttcgggcttc gccgcccgcc aactggtgaa aaatattcgc
    15601 aaacaggacg ccactattcc attaaccctg attgccgccg acagcatgga tgagtacaac
    15661 aaacctgacc tcagccatgt tatcagtcag gggcaacgtg ccgatgacct tacccgccag
    15721 acggcgggtg aatttgccga gcagtttaat ctgcacctgt ttccacaaac ctgggtgacg
    15781 gatatcgatg ccgaagcccg tgtggtgaaa agccagaata atcagtggca atacgacaag
    15841 ctagtactgg caaccggtgc cagtgccttt gtcccgcctg tgcctgggcg tgagttaatg
    15901 ctgacgttaa atagtcagca agagtatcgc gcctgtgaaa cgcaactgcg ggatgcccga
    15961 cgcgtgttga ttgttggcgg tggtttgatt ggtagcgaac tggcgatgga tttttgtcgt
    16021 gcaggcaaag cggtcacgct aatcgacaac gctgccagta ttctggcgtc gttaatgcca
    16081 ccggaagtaa gcagccgctt gcagcatcgg ttgacggaga tgggcgttca tctgctgttg
    16141 aaatctcagt tacaggggct ggaaaaaacg gattctggca ttctggcaac gctggaccac
    16201 cagcgtagta tcgaagtgga tgcggtaatt gccgccaccg gactgcgccc ggaaacagcc
    16261 ctggcacgac gcgccgggct gacgattaat cgcggcgttt gcgtggatag ttatctgcaa
    16321 accagtaatg acgatattta tgcgctgggc gattacgcgg aaatcaacgg tcaggtactg
    16381 ccattcctgc agccgattca acttagcgcg atggtgctgg caaaaaatct tctcggcaat
    16441 aacacgccgc tgaaactacc gacgatgctg gtgaaaatca aaacgccgga attaccgctg
    16501 catctggcag gcgaaaccca gcgtcaggat ttacgctggc aaattaatac cgaacgccag
    16561 ggaatggtgg ctcgcggtgt tgacgatgct gaccagcttc gcgcctttgt ggtcagtgag
    16621 gatcggatga aagaggcatt tggattgttg aaaacattgc cgatgtaggt gggctactgt
    16681 gcctaaaatg tcggatgcga cgctagcgcg tcttatccga cctacgggga cgcatgtgta
    16741 ggccggataa ggcgtttacg ccgcatccgg caatggtgtc caaatgcaac acgttttatc
    16801 cgttctggac ttcacccgct aaccaacgcg ccgcagcaat aaccccctgc cccagagaca
    16861 aaccgccatc acccgccggt aaactctgtg gaaagagcaa tgtgaaatca gcgagataat
    16921 gcgccagacg tgcacgcagc aaacggttat gaataacccc gccgctaaat accagcgtag
    16981 tgataccacg catcgtggcc tgctcacgca tcaacgcggc aaaaccctgc gccagcgcat
    17041 catgaaacgc ccacgcgcgt tgattaacct gtgcctgcca gttcagccac tgctgccaga
    17101 aagcggcgag atccagttga ttgtccacca gcggcatcgt caccggatgc gtcactccgt
    17161 ggcatgaggc tgcgagcgcc tccagagcac aagccgcttc accttcataa cttaacgtgg
    17221 ctggcgcaca gcccagtgcc gccgccactg catcgaacaa ccgcccacac gatgacgcca
    17281 gcggcgcgtt aattccacgc tcaatggccc gcgccagcac gctccagttt tgctgttgca
    17341 cacttgctgt ttcagggtaa ttctgccact ccggcacaaa gcgcaggcac tgcgccagca
    17401 ggtttcgcca cggctgcttc gctgccaaat cgccacccgg aagcgccact gcaggcaagc
    17461 cgcccaggtg ctcacattcg cgatagttca cccgcaggca ctcgccgccc cacaaagcgc
    17521 cgttctcccc cataccgata ccgtcgagcg tcaaagcaat gacatcaccg ccatccagcg
    17581 gccactgatg ctctgccaga cacgccgctg catgggcatg atgatgcagt accgtttgcg
    17641 tcggcagatt catttcacgc gcccactggc tggagacata gcccggatgc gcgtcatgca
    17701 caacgtattt cggggtaaaa tcgtagatgt tttgcatcag gcgtaacgct tcgcgccact
    17761 gcatctggat gccatcgtca cttaaatcgc ccagatgctg actcaacacc gcttgttcac
    17821 cgcgcaccag gcagaaggta tttttcagat ccgctccgag acacagcaca ggcggaacat
    17881 ttttaaagcc cggaggcaaa gccagcgcat ccggcacata cccccgcgaa cggcgcagca
    17941 tttcgccgct ttcgcgcacc accgaatcat ccatccgctg cacgatgtcg cggttatgta
    18001 tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc ctgttcgttg ctgatagctg
    18061 gtggtttacc gctcaggttg ccggaggtca tcaccagcgg gcattgcagt tcctgtaaca
    18121 gcaaatgctg gagcgggttc gcaggcaaca ttaccccgac ttcgttaagg tcaggggcga
    18181 tatcatcaca aagctcagga acgtattttt tatccaccag cacaatcggc gcggcgggcg
    18241 tggtaagcaa ctggcgcgca gcgtctggta aaccgtcagc cactggcaac atgaccgcca
    18301 gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc caccgcgtta ctgttacgtg
    18361 catcgcaggc aagatgaaat ccgccaatcc ctttgatggc gacaatgttg cccattttta
    18421 actgtgcgat agccgcctgt aatgacgcct cttgttctgc atgttcacca tgacttaccc
    18481 attcaagatg cgggccacac tccgggcagg ccaccggctg ggcgtggaag cgacgatcga
    18541 gcgggtcgcg gtactctttg tcacaagccg gacacaacgg aaacgccgcc atcacggtaa
    18601 acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg caggccgcag tgggtacagt
    18661 tgataaacgg ataacgataa cgccgttcgc ctggggtatt catttcggca aggcaagcag
    18721 ggcaagtagc ggcatccggg acaatttgcg tattcatggc acctcccgcg ctctggcgga
    18781 tggtgaactc agtgggcagt tgtgaccaga tgtacggctc acgctcgacg ctatcaatac
    18841 gcgccagcgg cgggcagtgc tgatgcaatt gaacaagaaa cgtttccggg tcttcccgca
    18901 gccggacttc tacgccatcg ccgtcattac agacatcgcc gtgaagattt aattgctgtg
    18961 ccagctgcca gacaaacgga cgaaaaccga cgccctgcac tttgccacga atacgcagtt
    19021 ggacaccgca agatgtgttt tttgccattg agttattccc gccatcatga attgcgtaac
    19081 ccgcccagcc ggacacgaca gcgtcgcatc cggcagtcac aggtcggcga taccgcccgc
    19141 tccgtattct acgaatattt ccgggaattc ctttgatgcc agaacagttc tgtaagattt
    19201 ttagaacatc agcgccgtac ggcggcgttt ttctgcactc agttgttcaa gtttattacg
    19261 atcgacacaa atcagcgcat gagtcgggca agccgccata cacgccgggc cgtcttcacg
    19321 atggttgcac aggtcacatt tattggcttc ggctttgtca gcccgtacat tcagccccgc
    19381 gccgctgttg cgaatcaccg gacgtaccac cacttccatc gcgccatacg ggcaagccac
    19441 aacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca tgaacaaacc ctttatcacg
    19501 gctgatagca ccattcgggc agacgttagc gcacggtgca tcttcacact gacggcaaac
    19561 tgtcgccgtg gaaatgttca cacctttaat gacatggata cgcggtaaaa atgtttccgg
    19621 ggtcagcgat gcacagtcct gattttcctg atgagaaacc acgcacgcca cttcacaggt
    19681 acggcaacca atacatttac tcgcgtcagc aatgatgaaa cggttcatca aattctccag
    19741 caatgacagt taatgcgccg atacattcac aaatcatgcc agtttttaat tcactgttat
    19801 ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc atcgacacta gtgacgatga
    19861 catgtgatga caatgtttat cgcgaaagag caatgagtga gtcgcggtgg atcagttttc
    19921 cgctgaaggt tttcggcggt gagaaatccc cgccatcgag cataaaaatc agccgtccaa
    19981 taatttcctg aatcatctca gtcaccggaa tttttacgct ggagagcgcc ggaacgatat
    20041 agggggcaat tgcgatatca tcgaatccga taactgacac ctgctctggc accgctacgc
    20101 cgcgctcgtg caacgctttc atcgcaccta tcgccatatc gtcgttactg gcaactaacg
    20161 cgctaaattt agccccacgt tcgagcaacg tttctacccc ttcggccccg ctggcgggcg
    20221 tccatttacc gttagcgata agtttttcat tgagcgcaat accatgctgc gccagcgcgt
    20281 ctttataccc agcaagacgt tcaatgctgg tgggggaatc catcgagccg gtaaggaaag
    20341 caatctcctg atgtccggcg tttatcaact ctgccacggc attaaagctg gtctgtttat
    20401 gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg gcgattaagc accattatcg
    20461 gctgactgtg cgcgtcaatg atgtcatcga tctcatccac gcttaaaaaa cgcgggtaaa
    20521 tcataatggc gtcgcagcgc aaatccagca gatactgaat cgcctggcgc tcttcttcgg
    20581 cgctgtgttt accatctgcc aatagcaact gccgcccttt ctcttccgcc attcgcgcgg
    20641 catgaaagag taattcacta aaataaatgc cgtggtaaag cgtgttggtc actaccagcc
    20701 ccagcgtctg agtactcttc gccgacagat tgcgcgccag caagtttggg cggtaaccgc
    20761 tctcttctac cgcctgaaac acgcgatctt tagtctcctg gctgacgtag ccattacctg
    20821 aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg cttcgccact tccagcatcg
    20881 tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt gtactgcacc gtttgccgat
    20941 tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg gtttcacaaa tataaaaaat
    21001 gaataattaa ctctcttgct tatttagtga caactattca tgattttgtg aaacgggttt
    21061 cctgattccg tttcagcatc ggcatttttc cgttacgtcg actgataaca actacatcta
    21121 ccctactgat aacaggataa aatccgatgg ccaaaaatta tgcggcgctg gcacgctcgg
    21181 tgatagcggc actgggcggc gttgataaca tctcggcggt cacgcactgt atgacgcgtt
    21241 tgcgctttgt tatcaaagat gaccagctta tcgacagccc gacgttaaaa accatccccg
    21301 gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt gattatcggc aataccgttt
    21361 cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga tatgcagccc gcacagcccg
    21421 tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg gatcctcgat gcgctgatcg
    21481 gcaccatgtc accgctgatc ccggcgatta tcggcggatc gatggtcaaa ctgctggcga
    21541 tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc gaccttaacc attctgaatg
    21601 tgattggtga cggtgctttc ttcttcctgc cgctgatggt cgcggcatct gccgccatca
    21661 aatttaaaac caatatgtcg ctggcgattg ccatcgcggg tgtgctggta catccgagtt
    21721 ttattgaact gatggcgaaa gcggcctagg gtgaacatgt cgaatttgcc ctgattccgg
    21781 tcaccgcggt gaaatacacc tacacagtga tcccggcgct ggtgatgacc tggtgcctgt
    21841 catatatcga acgctgggtg gatcgcatta caccggcggt gaccaaaaac ttcctcaagc
    21901 caatgctgat tgtgttgatt gccgcaccgc tggcaatcct gctgattggc ccgattggta
    21961 tctggatcgg tagcgccatt tcggcgctgg tttacaccat tcatggttat ctgggctggc
    22021 tttcagtcgc cattatgggc gcgctgtggc ctctgctggt aatgaccggg atgcaccgcg
    22081 tctttacgcc aaccatcatt cagaccattg ccgaaaccgg caaagaaggg atggtcatgc
    22141 cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc actggcggtg gcgtggaaaa
    22201 cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc ggcatcagcc attatggcgg
    22261 ggatttccga accggcgtta tactgcgtgg cgatccgcct gaaacgtccg cttatcgcca
    22321 gtcttatcag cggttttatt tgcggcgcgg ttgccggtat ggcggggctt gccagccact
    22381 caatggcagc gccggggcta tttaccagcg tgcaattctt cgatccggcg aatccaatga
    22441 gcatcgtctg ggtgttcgca gtcatggcgc tggcggtggt gctgtcgttt atcctcacac
    22501 tgttgctcgg ctttgaggat attcctgttg aggaagcggc tgcccaggcg cgaaagcatc
    22561 agagcgtaca accgaccgtc gccaaagaag taagtcttaa ttgaggatga aaatgtcagt
    22621 atttccagaa ggttttttat ggggcggcgc gcttgccgcc aaccagtctg aaggtgcgtt
    22681 ccgtgaaggc ggaaaagggc tgaccaccgt cgatatgatc ccacacggcg agcatcgaat
    22741 ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc gatgacgagt tttatcccag
    22801 ccatgaggcg acggattttt atcatcgtta taaagaagat atcgccctga tggcagagat
    22861 gggattcaag gttttccgta cctcaattgc ctggagccgt ctctttccgc agggcgatga
    22921 aatcacgccc aatcagcagg gcattgcttt ttatcgttct gtctttgaag agtgtaaaaa
    22981 gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat gtgccgatgc atctggtcac
    23041 cgaatatggc tcttggcgta accgcaagct ggtggagttt ttcagccgct acgccagaac
    23101 ctgctttgaa gcatttgatg gtctggtgaa atactggcta accttcaatg aaatcaacat
    23161 tatgttgcat agcccgttct ccggcgcggg tctggtgttt gaagaaggtg aaaatcagga
    23221 tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc agtgcgctag ccgccaaaat
    23281 cgcccatgag gttaacccgc aaaatcaggt gggctgtatg ctggcgggcg gcaacttcta
    23341 cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg gagaaagatc gggaaaacct
    23401 gttttttatc gatgtgcagg cgcgcggcgc gtatccggct tactctgccc gcgtatttcg
    23461 cgaaaaaggg gtaaccatag ataaagcgct gggcgatgat gagatcctga aaaacaccgt
    23521 cgattttgtc tctttcagct attacgcctc gcgctgcgcc tcggcggaga tgaacgccaa
    23581 caacagcagt gcggcgaacg tggtgaaatc gctgcgtaac ccgtatctgc aagtgagcga
    23641 ctggggctgg ggaattgatc cactcggtct gcgtatcacc atgaatatga tgtacgatcg
    23701 ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc gcaaaagatg aacttgctgc
    23761 caatggcgag attaacgatg actatcgcat cagctattta cgcgaacata tccgcgcaat
    23821 gggcgaagcg attgcagatg gcattccgct gatgggctac accacatggg gctgtattga
    23881 tttagtttcc gcctctacgg gtgaaatgag caaacgctac ggttttgtct acgtagaccg
    23941 tgacgacgca ggcaacggca cgctgacgcg cacacgtaag aaatcgttct ggtggtataa
    24001 aaaagtgatt gccagtaatg gggaagattt agagtaagta acagtgccgg atgcggcgtg
    24061 aacgccttat ccggcctaca tgtcgcacga accaggaagg cccgataagc gcatcgggca
    24121 atttagcatt tggcatcaga gctggttcac cccatcaaga acatccctgt cctgattcct
    24181 taatgaaaaa ctactcttct tccaccgcta actgcgcgaa gccgccattc ccttcccagc
    24241 cttccagtcg ttgataaacg gtttctaccg catctttaat cggctgggtc atcgggtagt
    24301 aaaagccgac gatatccggc tgtatgccga ggaaaatcac ttcgccaata tcttctttca
    24361 actggtcgat aagatagttc agaggcatgt tatgggtagt catcataaac atctcggcga
    24421 tatcatccgg gtcgatgatg cggatctcgc cggggtttag ccccatatcc gtggcgtcga
    24481 caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac gatgtcgttt tccggtgcac
    24541 taccgccgtc aatcaccacc cagttacctt tcggcgcggc ggcgcacttt tccgccagca
    24601 gcggacctgc gccatcatcg cccatcatgc tattgccaac acagagtaaa acgtcagtca
    24661 cgcaatctcc tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt
    24721 aacagcattg tactccattc ctgctgctgc gaagtttgcg ctgcccgggc tttatcgaat
    24781 gcgttggcca gcatcggcac atggttaatg tcgatgacga tctcaccgta cttcggtacg
    24841 ccttccattt tgcggcgggc ttcactgccc gcctccagag tggcaatcca tgccagatat
    24901 tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga taaccccaag gtggtgacca
    24961 atcgccaggc tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca
    25021 ataaatttac ggctcagttg actgaacacc accttttcac tcatcggata cgcacctctt
    25081 ctacaacatg attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca
    25141 gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt caggaagtca tcggcaatct
    25201 gacgaccgta acgataaccc gccaggcggc gtgcttcgcg atccactttc acgcgcagct
    25261 gctgcaccat atcgccatgc aggatctccg ccggttgttc atccagttca cccggcccac
    25321 gggcgtgaat tttctgctcc agcaggccga gcgccattgc aaagccgtac agcgtggcgg
    25381 caggcgttgg cgggcagcca gggatataaa catccacagg gacaatttta tccgtaccgc
    25441 cccacacgca gtagagatcg tggaagatcc cgccgctgtt accgcaggca ccgtaggaga
    25501 tacaaatttt cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac
    25561 gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg cggtgaagga acgactttaa
    25621 tgccgaagcg ttctgcatca aacagcggcg ataaagtggc gaaaatttcg atttcgcagc
    25681 cgttgcagcc gccgcagtcc acgcggtaaa cataggcaga acgtttgatt tttttcagta
    25741 acgacgcctt catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg
    25801 cgtcacgggg gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc
    25861 aatacggtcg gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct
    25921 ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta agcagcgcaa tggcgtagtc
    25981 gatctctttc tggacggcga aaggacgatt gcagacgcga cagttgcaca gcgcgaagcg
    26041 agactgttgc aggaagtctt ctttcttcca caccgccagt tcgtactctt gcgacagctt
    26101 aatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag aagatgcagc gcccgagatt
    26161 aaactcccag gccagctctc ctgtggcgag gtcagtttca accgttaagg cgtttgacgg
    26221 gcaggcattg acgcaggccg cgcagccgat gcactgctgc gggttctgct ctggcttacc
    26281 acggaagttt ttatcaaccg caatcggctc cagcggataa gacgaggtcg ccgtgccggt
    26341 tttgataact tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt
    26401 acgctcaatg ctgtagcgct cgagttcttt gtacggcacc actttgctct tcttcttacg
    26461 tacatcgacc acagtcatgc ggtcggtaca ggagtagcaa gggtccaggc taccgataat
    26521 cagcggcgca tcggaaacag tgttgccacg cagcatgtag cgcagggtcg gccagttcgc
    26581 gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccag tcatgctcca
    26641 gtggatatca tcaccgcgcg gcgcttcggc aaagcccagc gcgaaacggt gcggaatgta
    26701 ggtaaagcct tccaccatca gcgggccacc cggcaggtta tccagaccgt agtcgatcat
    26761 gttcagcgcg gtatagactt cgttgatacg cactttcaga cgcgaaataa cgtcgcagcc
    26821 ctgctcgctg tggacttcca ctggcagcag gccataacca acaaacgggt gatcagcgcg
    26881 ggtatcgcga gcgtgtccgc tggcgcggac catcgggcca acgttactga aatcgcgggc
    26941 aatttccggg tccagacgac caatgccgac ggtgcgctgt tccatgttcg gtgtgctcag
    27001 cagcacatcc accagctcct gcacttcacg acgcatctgt tgcgccagct ggtgggtctg
    27061 gatcatgtca tctttcaaca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt
    27121 tttacgcgca ccggtgagga tctcggccat tttcatggag gtttcacgca cgcggaagaa
    27181 ctgcataaag ccgtagtcga agccggtaaa gtggcaggcg aggccgaggt tgagcagatg
    27241 cgagtgcagg cgttccacct ccagcagaat ggcgcggatc atctgcgcac gttctggcac
    27301 ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg gtgctgtggg cggagccgca
    27361 gatcccgcac acacggtcag agaggaaggt cacttcgtta taacccatgc gggtttccgc
    27421 cagtttttcc ataccgcgat ggacatagaa caagcggtag tctgcgtcga taatgttttc
    27481 gccatcgacg aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat
    27541 cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcacc
    27601 ggtggtcggt gccggacgct gacgataatc catgctgtct ttacgcagcg gataaagttc
    27661 atccggccag tcatccggca gcaccagacg gcgttcatcc ggcagaccaa ccggaatcaa
    27721 accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc gccggaacgc gcggcgtcac
    27781 ggacggatac tccggtttgt tggcatcaac ttcgacgcga accgttaccc agcacttagt
    27841 ccccttctcc atcgacagca cgtaataaac ggcgtaatga ccattcagtt tgcgttcgtc
    27901 gttaccaaaa agcaccgaca gccagccccc ctgcttgtag tagagaaact ccaccacttc
    27961 cggcagatag ttcaccttta cggtgacagt cagctgatct ttggtctgcc aggcgtggtc
    28021 cagcacgacg cccggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc
    28081 ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat
    28141 gcaaagccaa acccagccca ggtaatgcgc ggagtaatat caagacgcag acgggccatg
    28201 ctgttttcga acagcgcgat aaccaggacg ccgaccacca gttttacgat ggcaatcacc
    28261 agcgccagca gcagtccacc ggcggtgaag gtttccattt gtccccacgg aataaacacc
    28321 ccgacgaaca tctgcaacac caccagctgt ttcaggctga taccccattt catgacgcca
    28381 aagccgctgc cgctatactc agagagcgga ccttcctgca actcctgctc ggcttccgcc
    28441 aggtcgaacg gcagtttgcc catttcgata aaagtggcga acgcacaggc gcagagcgcc
    28501 agcaccagcg ggatgctttg gctcaacggc cagtgataaa cggtgtcggt aatgttgctg
    28561 atgttggtgg aaccggcaac ctgtgcggca acccacagac caagcagcaa catcggttcg
    28621 accagcacgc caagcatcgc ttcacggctc gcaccgatag cggtaaacgg gctaccggta
    28681 tccagaccgg aaatggcaaa gaagaaacgc gcgatggcga agagatacag taaggtgatc
    28741 aaatcgccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtagcgata
    28801 gtcagcatga cgcccaccat cacatacggc gtcaggcgga acacccagcc agaggcatcc
    28861 gggccaacgc tctggcggcc cagcagtttg ataatgtcgc gatactcctg caacacgccc
    28921 ggcccgcgac ggttatgcag acgggcgcgc gccacgcggg taataccgga gagcagcggc
    28981 gcaacggcaa ataacaccag cgcctgaatt aacggatata aaacactcat tctcaggctc
    29041 ctcgtgaaac aataatcacc accagtaccg ccagttcaac cagcgccatc cggcggaaca
    29101 gcaacgcact accttcgcac tgccagcccg gcaccagcga caccggattc agccatttgc
    29161 gcagtttcag cactggcgca aacgcctgtt taaccggcat ggcaaaaccg tgagcggtaa
    29221 tcaccattga tttttcatgg tcgtagccgc acacccaggc cgcaccgcgg gaacgtgacg
    29281 gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca
    29341 gcagcaatgt gatcattggt tgagaaacgg tggtgttagc aggctccaga ggcagaggta
    29401 cggcagcaga gagcatcggc agcagccacg gcgcagcaac accgccaatt acgcagcaaa
    29461 tcgccagtgc cactacgctt acgctcatca ggagcggcgc acaggtggcg ttttcggctt
    29521 ctttggtacg cggcgcgccg aggaacgtga cgccatagac tttcgccata cacatcaccg
    29581 ccagcgcacc ggtaattgcc agacccacag cgagcagcgg ccccagcaga cgggcaacaa
    29641 acgcgccact attgctcagt ttgaaaaagg attgatagat aacccattcc ccggcaaacc
    29701 cgttcagcgg cggcagcgca gccattgcca tcaacccgac taacatggcg atggagataa
    29761 ccggcatttt cttgccaata ccaccgagtt tttcgatatc gcgatgaccg gtacggaacc
    29821 agacgctccc cgccccgagg aacagtacgc ttttgaacag gctatggtta agcagatggt
    29881 acagaccacc gaccaggcca agagcaatca gcgccggttg ttcgagcgcg atacccgtta
    29941 cgccagcgcc cagccccagc cccagcccca gccccagccc cagccccagc cccagcagga
    30001 taatgccgat attttccagg gtgtggtaag ccagcaggcg ctggatgttg tgctccatca
    30061 gcgcatacag accaccgaca aacgcggtga tcatgccgag caccagcagc acgatccccc
    30121 accacagcgg tgcattaccg cccagcagtg acagggttaa aatgcccagc aggccaattt
    30181 tcatgactac cgtagaaaac aacgcggcag ctggcgcaga ggcgttagca tgtgcctgcg
    30241 gcacccagcc gtgcagcgga ataatcccgg cgagcaggcc aaagccaatc acaccgagca
    30301 gccagatatc ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca
    30361 gcgtgccata acgctgccac agcagccagc aagcaatcgc cagcagcaga gtgccaagac
    30421 gccccagcgc aaaccacagt ttgccctctt tgctgttgct ggtgaggaac accgcacaca
    30481 aggccatgac ttcggccatt accacgaaca tgccgaggtt gctggcgatg acggcgcaga
    30541 cggcggcagc catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat
    30601 gccagtcaat gttgtagagg ctgacaaaca gaccgcacag accgagcgta atcagccaaa
    30661 tcgcgttaag cggggagatt tgcacatcgt agcttaccag cgacagcgca ccgctcacgc
    30721 caaccgcgcc agtcagcaca gtgaagcccg cggctgccgt atacaggcta ccaaccgcgc
    30781 cgccaattcc ggctatccag ccacttagcg tcttttgaaa agaaaagaga aatgccagaa
    30841 cagcggcggc gacaaaccac accacgccgc tattgatcag ggaaattgcg ctcatttagc
    30901 ctctccactt tgagcctgct gaaacaaggt gagatcgcca aagtccgtat taaaagtcag
    30961 ctcacgctta cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt
    31021 ggtcgggcac atccgcacgc aggccggacc ttgttcatca aagctacaaa ggtcacattt
    31081 gacggcgatc gcgcgaatac ccggcaccca gtcaagcagc gtactgatac gcgccggagc
    31141 aggcggtgcc ggtggcgctt tcggggtatt ggcatttgca ggaatatcca gcggacggct
    31201 gccggaaaat tcaattgcgc caaacgggca ggcgatgccg cacagctggc agcttacgca
    31261 caggctttca ttcaactgca cggccccatc aacgcgggtg atgacgttaa ccgggcagac
    31321 caccgcgcag ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt
    31381 cagcatcact ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca
    31441 ggctgcctca caagtgtggc agccgataca gagcgtggag tcagcaatta caaaacgatt
    31501 caccaggcat tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt
    31561 ttcgacactc atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag
    31621 cctcagctca tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat
    31681 tgttttcaat ggcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt
    31741 ccagaacctg ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca
    31801 gttgcgcctg cgcaatccgc tgtttgtctt cgccctcttt tgtttcgacg taatactcga
    31861 tggtgtggct gttaaagcca tccgccaggg tgacgtaaat acgaaaatgt tcaaaaagga
    31921 cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata
    31981 acgtgatccc ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt agacgacgat
    32041 gccgctgtgc gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac
    32101 ccgtttaaca gagatgccag ctttaagcat tttctgtgcc aatttttaat ttattgttat
    32161 taaaacgatt tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat
    32221 catcgacatt attcaccgca gggataatca acactggcac aattattgct tgtagctggc
    32281 aatagttaat gggaggcgat atgcacgcaa taaccctctg ccaacgggca ctggaattga
    32341 tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg
    32401 gcgcattttc ttgcgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg
    32461 gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca agaggtcgaa tgctggtgtg
    32521 aaacatgcca acagtatgtg acgctattga cccagcgcgt ccgccgctgt ccacagtgtc
    32581 atggtgacat gctacagatt gtggcagacg acggtttaca gattcggcgg atagaaatag
    32641 accaggagtg agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg
    32701 gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc
    32761 cgaagatgaa aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag
    32821 gcgacctgca ttacggtcat ggcgaagcgg gcactcacgc accgggcatg agccagcgtc
    32881 ggatgctgga agtcgaaatt gacgtgctgg acaaaaacaa ccgtctggct gaacgcaacc
    32941 gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct ggtttccagc ccaggttccg
    33001 gtaaaaccac cctgctgacg gaaaccctaa tgcgcctgaa agacagcgtt ccgtgcgcgg
    33061 ttattgaagg tgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggcacac
    33121 cagcgattca ggtgaacaca ggtaaaggct gccatcttga cgcacagatg attgccgacg
    33181 ccgcaccgcg tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc
    33241 tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa agtggcggtg ctttccgtta
    33301 ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc
    33361 tgctcaacaa agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcatcgcct
    33421 gcgcccgcga agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggtgaag
    33481 ggatggacca gtggctgaac tggctggaga cacagcgatg tgcataggcg ttcccggcca
    33541 gatccgcacc attgacggca accaggcaaa agtcgacgtc tgcggcattc agcgcgatgt
    33601 cgatttaacg ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt
    33661 actggtacac gttggctttg ccatgagcgt aattactgaa gccgaagcac gcgacactct
    33721 cgacgcctta caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga
    33781 ggaaaaataa tgcgttttgt tgatgaatat cgcacgccgg aacaggtgat gcagttaatt
    33841 gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt
    33901 atggaagtgt gcggtggtca tacccacgct atttttaaat tcggcctcga ccagttactg
    33961 cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt
    34021 agaatcgaca cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc
    34081 gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc
    34141 gatgtgcgca tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc
    34201 cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact
    34261 ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact tcttctgtca gcacattacg
    34321 cttatcccga cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc
    34381 gcgccgggcc acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat
    34441 tttcatcgtc cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtg
    34501 atgctggtgg agcagaaaat agaggcccac agcaaggtag agaatcagta tcgtcgggtg
    34561 gtaccggatg ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac
    34621 ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg
    34681 gattatcaac gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat
    34741 gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg
    34801 ctgtttggta acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa
    34861 ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc
    34921 aactcgccca cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg
    34981 aagcctttgc caacccgtgg ctggcagagc aggaagacca ggcacgtctt gatctggcgc
    35041 agctggtagc agaaggcgat cgtctggcgt tctccaccga cagttacgtc attgacccgc
    35101 tgttctttcc tggcggtaat atcggcaagc tggcgatttg cggcaccgcg aatgacgttg
    35161 cggtcagtgg cgctattccg tgctatctct cctgtggctt tatcctcgaa gaaggattgc
    35221 cgatggagac actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca
    35281 ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta
    35341 tcaacaccgc tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa
    35401 ccgcaggcga tgtattgctg gttagcggta cactcggcga ccacggggcg actatcctta
    35461 acctgcgtga acaactgggg ctggatggtg aactggtcag cgactgcgcg gtgctgacgc
    35521 cgcttattca gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg
    35581 gtggtgtaaa cgcggtggtt catgagttcg cggcagcctg cggttgtggt attgaaattt
    35641 ctgaatcagc actgccggtt aaacctgctg tgcgcggcgt ttgcgaactg ctgggactgg
    35701 acgcccttaa ctttgccaac gaaggcaaac tagtaatagc tgttgaacgc aacgcagcag
    35761 agcaagtgct ggcagcgtta cattcccatc cactggggaa agacgcggcg ctaattggtg
    35821 aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta tggtgtgaaa cgaaccctcg
    35881 atttaccaca cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag
    35941 ttagtcaatg accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat
    36001 acaccgatga gtgatctcgg acagcaaggg ttgttcgaca tcactcggac actattgcag
    36061 cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc
    36121 gccgacaacg cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgca
    36181 tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg
    36241 gtacgccgca ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa
    36301 acctggtcgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg ccactggcgg
    36361 cggaagggca tatttttggt ggctgtgaat ttattcgtta tgacgatcgt ccctggagcg
    36421 aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc accgaacaaa
    36481 tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag cgcgataact
    36541 tccgcatcct ggttaccatt accaacgcgg tgctttccca cctggatatg gacgaactgg
    36601 tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc agtatcgtct
    36661 tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt gataaacaac
    36721 atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca
    36781 aaagtaaaga gatgctgctg atcaatctcc acgagcggga cgatttagcc ccctatgaac
    36841 gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt
    36901 ctggcgacac catgctgggc gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca
    36961 ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg
    37021 ccctcaccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa aacctcgtcc
    37081 tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc cgcagcgaag
    37141 ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt accgtgctga
    37201 tcctcggtga aactggcacg ggtaaagagc tgattgcccg cgcgatccat aatctcagtg
    37261 ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg
    37321 aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccagcgcc cagcgcattg
    37381 gtcgttttga actggcggat aaaagctccc tgttcctaga cgaagtgggc gatatgccac
    37441 tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca
    37501 gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc gatctgaaaa
    37561 aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac gtattcccga
    37621 ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg aaagccttta
    37681 ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattccagcc gagacgctgc
    37741 gcaccttgag caacatggag tggccgggta aagtacgcga actggaaaac gtcattgagc
    37801 gcgcagtatt gctaacacgc ggcaacgtgc tgcagctgtc attgccagat attgctttac
    37861 cggagcctga aacgccgcct gccgcaacgg ttgtcgctca ggagggcgaa gatgaatatc
    37921 agttgattgt tcgcgtgctg aaagaaacca acggcgtggt tgccgggcct aaaggcgctg
    37981 cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg
    38041 ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc ggcatactat
    38101 tcacgagctt ttttcggacg atatttttcc ggcagttctg gcaccggacg cttgtcatcg
    38161 atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc ggcccaacgc
    38221 ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg acactgctta
    38281 caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc gtaaacaaac
    38341 aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg ataaagatcg
    38401 atcatttttt taatcgtcag tttttcacga gagatacgct tgccggacat gctgcctcca
    38461 tactcttaag gtgtatttat attacaactt aatttgaaag gggactatgg ctctgaagga
    38521 ggaggtttat tcaaccgacg caattttgcg tacttgcttc ataagcatca cgcaaaagct
    38581 gcaaaacagc atctttcccg gaaccagcat caagaactcg ccgttcgctt cttcccctga
    38641 aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata aaaaccatca
    38701 caccccattt aatatcagga accggacata accccatgag tgcaatagaa aatttcgacg
    38761 cccatacgcc catgatgcag cagtatctca ggctgaaagc ccagcatccc gagatcctgc
    38821 tgttttaccg gatgggtgat ttttatgaac tgttttatga cgacgcaaaa cgcgcgtcgc
    38881 aactgctgga tatttcactg accaaacgcg gtgcttcggc gggagagccg atcccgatgg
    38941 cggggattcc ctaccatgcg gtggaaaact atctcgccaa actggtgaat cagggagagt
    39001 ccgttgccat ctgcgaacaa attggcgatc cggcgaccag caaaggtccg gttgagcgca
    39061 aagttgtgcg tatcgttacg ccaggcacca tcagcgatga agccctgtta caggagcgtc
    39121 aggacaacct gctggcggct atctggcagg acagcaaagg tttcggctac gcgacgctgg
    39181 atatcagctc cgggcgtttt cgcctgagcg aaccggccga ccgcgaaacg atggcggcag
    39241 agctgcaacg cactaatccg gcggaactgc tgtatgcaga agatttcgcc gagatgtcgc
    39301 tgattgaagg ccgtcgcggc ctgcgccgtc gcccgctgtg ggagtttgaa atcgacaccg
    39361 cgcgccagca gttgaatctg caatttggca cccgcgatct ggtcggtttt ggcgtcgaga
    39421 acgcgccgcg cggactttgt gctgccggtt gtctgttgca gtatgcgaaa gatacccaac
    39481 gcacgaccct gccgcatatt cgttctatca ccatggaacg tgagcaggac agcatcatta
    39541 tggatgccgc gacacgtcgt aacctggaaa ttactcagaa cctggcgggc ggtgcggaaa
    39601 atacgctggc ttctgtgctc gactgcaccg tcacgccgat gggtagtcgt atgctgaaac
    39661 gctggctgca tatgccagtg cgcgataccc gcgtgttgct tgagcgccag caaactattg
    39721 gcgcattgca ggatttcacc gccgggctac agccggtact gcgtcaggtc ggcgaccttg
    39781 aacgtattct ggcacgtctg gctttacgaa ctgctcgccc acgcgatctg gcccgtatgc
    39841 gccacgcttt ccagcaactg ccggagctgc gtgcacagtt agaaactgtc gatagtgcac
    39901 cggtacaggc gctacgtgag aagatgggcg agtttgccga gctgcgcgat ctgctggagc
    39961 gagcaatcat cgacacaccg ccggtgctgg tacgcgacgg tggtgttatc gcatcgggct
    40021 ataacgaaga gctggatgag tggcgcgcgc tggctgacgg cgcgaccgat tatctggagc
    40081 gtctggaagt ccgcgagcgt gaacgtaccg gcctggacac gctgaaagtt ggctttaatg
    40141 cggtgcacgg ctactacatt caaatcagcc gtgggcaaag ccatctggca cccatcaact
    40201 acatgcgtcg ccagacgctg aaaaacgccg agcgctacat cattccagag ctaaaagagt
    40261 acgaagataa agttctcacc tcaaaaggca aagcactggc actggaaaaa cagctttatg
    40321 aagagctgtt cgacctgctg ttgccgcatc tggaagcgtt gcaacagagc gcgagcgcgc
    40381 tggcggaact cgacgtgctg gttaacctgg cggaacgggc ctatacccta aactacacct
    40441 gcccgacctt cattgataaa ccgggcattc gcattaccga aggtcgccat ccggtagttg
    40501 aacaagtact gaatgagcca tttatcgcta acccgctgaa tctgtcgccg cagcgccgta
    40561 tgttgattat taccggcccg aacatgggcg gtaaaagtac ctatatgcgc cagaccgcac
    40621 tgattgcgct gatggcctac atcggcagct atgtaccggc acaaaaagtc gagattggac
    40681 ctatcgatcg catctttacc cgcgtaggcg cggcagatga cctggcttcc gggcgctcaa
    40741 cctttatggt ggagatgact gaaaccgcca atattttaca taacgccacc gaatacagtc
    40801 tggtgttaat ggatgagatc gggcgtggaa cgtccaccta cgatggtctg tcgctggcgt
    40861 gggcgtgcgc agaaaatctg gcgaataaga ttaaggcatt gacgctgttt gccacccact
    40921 atttcgagct gacccagcta ccggagaaaa tggaagacgt cgccaacgtg catctcgatg
    40981 cgctggagca cggagacacc attgccttta tgcatagcgt gcaggatggc gcggcgagca
    41041 aaagctacgg cctggcggtt gcagctctgg caggcgtgcc aaaagaggtg attaagcgcg
    41101 cacggcaaaa actgcgtgag ctggaaagca tttcgccgaa cgccgccgct acgcaagtgg
    41161 atggtacgca aatgtctttg ctgtcagtac cggaagaaac ctcgccagcg gtcgaagctc
    41221 tggaaaacct ggacccggat tcactgactc cgcgtcaggc gctggaatgg atttatcgct
    41281 tgaagagcct ggtgtaataa caattcccga tagtcttttg ctatcgggaa tattaacgac
    41341 aactgacgaa taaaataaaa acaccctgta taataggaaa gcttatttta cagggtaaaa
    41401 ccatgccatc tacacgctat caaaaaatta atgcccatca ctatcgccat atatgggtcg
    41461 ttggtgatat tcatggtgaa tatcaattat tacaatcccg cttacatcaa ctctcttttt
    41521 gccctgaaac cgacttactt atttctgtcg gcgataatat tgatcgtggg ccggagagtc
    41581 ttgacgtcct gcgcctgcta aaccagccct ggtttatctc cgttaaaggc aaccacgaag
    41641 caatggcgct ggatgcgttc gcgaccggcg atggcaatat gtggcttgcc agcggtggtg
    41701 actggttttt cgatttaaat gattcagagc aacaagaggc aatagatctg ttgctgaaat
    41761 tccatcacct tccacatatt attgaaatca ctaacgacat aataaaatat gtcatcgcac
    41821 atgcagatta tccgggtgat gaatatctct ttggcaaaga aatagcagag agcgaattac
    41881 tctggcctgt ttatcgtgtg cagaaatcgc ttaatggcga gttacaataa ataaacggcg
    41941 ctgattattt tatatttgga catatgatgt ttgataacat tcagacgttc gctaaccaga
    42001 tttatattga taccggatcg ccgaaaagtg ggcggctgtc attttataaa ataaggtagt
    42061 ctcatgcttc ttctgtgaag catgagtaac cagatgttat tgcaggccat tattcatttt
    42121 tcgctaccag caaagagaga tcctgcttcg ccagcgcgcg actggcactc tccggcaaac
    42181 cgtcgtctgt aataatctga tcaaactcgc ttaatggtaa cgccagccat gtcgccacct
    42241 gaccatattt cgtcgcatca cagaccaaaa ctcgctggcg gctggcacta gcaatcgccc
    42301 gtttcaccgt gactttatct tccgctggcg tagaaatccc ccgcacactc catgacgatg
    42361 cagaaataaa agcctgatca atcatcaggc tgcgcagcat ggtcgcagcg gcttccccga
    42421 cacaggaacg gttttcccga cacactgcac cgccagtgtg aataattgtg caattactgt
    42481 tgtcgagcaa gtagttcgca ataacgaaat cgtttgtgac cacagtcagt gactccatgt
    42541 gaatcagatg ctgtgctatc gctaacgtgg ttgttcccgc atccagatag atacaacttc
    42601 ccggctgaac aagacttgcc gccagcttgc caatagccgc tttttgcgtc attgccagcg
    42661 cagtttttac ctgatgagaa ggttcatgcg ccacgcgtcc cggagactgg acgcctccgg
    42721 acaccagcac aacggctccc tgctgctcca gtttttgtaa atcccgacga atggtcatat
    42781 gtgacacatt cattctgtcc gttagttcag caatactgac aatgcctttt tcagctacca
    42841 tctcaaggat gatttggcga cgctctacgg gtatcaactt ttgctccttc ctttgtcctg
    42901 ctgacattct acgctatttg cctgcgaaac gtgcgcggcg caactaacgc ttagttcaca
    42961 taaaataaca cacaatgtta atttatgtga atcagctcac catatcgtta tcttccagcg
    43021 cttatattca caatatcaaa caaaatatca cttaaattaa caaggagagc agatgaaaac
    43081 gggatctgag tttcatgtcg gtatcgttag cttagggtca atgggaatgg gagcagcact
    43141 gtcatgtgtc cgcgcaggtc tttctacctg gggcgcagac ctgaacagca atgcctgcgc
    43201 tacgttgaaa gaggcaggtg cttgcggggt ttctgataac gccgcgacgt ttgccgaaaa
    43261 actggatgct ctgctggtgc tggtggtcaa tgcggcccag gttaaacagg tgctgtttgg
    43321 tgaaacaggc gttgtacaac atctgaaacc cggtacggca gtaatggttt cttccactat
    43381 cgctagtgct gatgcgcaag aaattgctac cgctctggct ggattcgatc tggaaatgct
    43441 ggatgcgcca gtttctggtg gtgcagtaaa agccgctaac ggtgaaatga ctgtcatggc
    43501 ctccggtagc gatattgcct ttgaacgact ggcacccgtg ctggaagccg ttgccggaaa
    43561 agtttatcgc ataggtgcag aaccgggact aggttcgacc gtaaaaatta ttcaccagtt
    43621 gttagcgggc gtacatattg ctgccggagc cgaagcgatg gcacttgcag cccgtgcggg
    43681 gatcccgctg gatgtgatgt atgacgtcgt gaccaatgcc gccggaaatt cctggatgtt
    43741 cgaaaaccgg atgcgtcatg tggtggatgg cgattacacc ccgcattcag ccgtcgatat
    43801 ttttgttaag gatcttggtc tggttgccga tacagccaaa gccctgcact tcccgctgcc
    43861 attggcctca acagcattga atatgttcac cagcgccagt aacgcaggtt acgggaaaga
    43921 agacgatagc gcagttatca agattttctc tggcatcact ctaccgggag cgaaatcatg
    43981 atcaagattg gcgttatcgc cgatgatttt accggcgcga cggatatcgc cagttttctg
    44041 gtggaaaacg gtctaccaac ggtacaaatt aacggtgttc caacaggtaa aatgccggaa
    44101 gcaatcgacg cactggtgat cagcctgaaa acgcgctcct gtccggtggt tgaagccaca
    44161 cagcaatcgc tggcggctct gagctggttg caacagcaag gttgcaaaca gatctatttc
    44221 aaatactgct ctactttcga cagtacggcg aaaggtaata tcggcccggt taccgatgca
    44281 ttaatggatg ctctcgacac gccgtttacg gtcttctctc cggccctgtc ggtcaacgga
    44341 cgtacggttt atcaggggta tttgttcgta atgaatcaac tgctggccga atccgggatg
    44401 cgccatcacc cggtaaatcc catgaccgac agctatcttc cccgtctggt tgaagcacaa
    44461 tccacagggc gctgcggcgt cgtttcggca catgttttcg aacaaggtgt ggatgccgtt
    44521 cgtcaagagc tggctcgctt acagcaagag ggctaccgct acgcggtgct tgatgcgctg
    44581 accgaacacc atctggaaat tcagggagaa gccttgcgcg atgccccact ggtaacgggc
    44641 ggttctggtc tggcgattgg cctggcccgg cagtgggcgc aagaaaacgg taaccaggct
    44701 cgcgaagcag ggcatccgct cgctgggcgc ggcgtagtac tctccggttc atgctctcaa
    44761 atgaccaacc gccaggtggc acattaccgt caaattgcac cagcccgtga agttgatgtg
    44821 gcacgctgcc tctcaaccga aactctggcc gcttatgcac acgaactggc agagtgggtt
    44881 ctgggccagg aaagtgtact tgctccactg gtttttgcca ccgccagcac tgacgcattg
    44941 gcagcaattc aacagcaata cggtgcacaa aaagccagtc aggcagtaga aactctgttt
    45001 tctcaactag cggcgcggtt agcagcggaa ggcgtgacac gctttattgt cgcaggcggt
    45061 gagacctccg gcgtagtcac acagagcctg ggcataaaag ggtttcatat tggcccaacc
    45121 atttcccccg gcgtgccgtg ggtaaacgca ctggataagc ctgtctcact cgcccttaaa
    45181 tctggcaact tcggtgatga agcctttttt tcacgagccc aaagagagtt tttatcatga
    45241 gcgatttcgc aaaagtagag cagtctttgc gagaggagat gacgcggatt gccagttcat
    45301 tctttcagcg cggctatgca accggttcgg ctggcaatct gtcgctgctt ttacctgacg
    45361 ggaatttact ggcgacaccg acaggttcat gcctgggcaa tctcgatccg cagcggcttt
    45421 ccaaagtcgc cgcggatggc gaatggttaa gtggtgacaa accctcgaaa gaggtgctct
    45481 ttcatctggc gctgtatcgc aacaatccgc gctgtaaagc ggtggtgcat ttgcacagca
    45541 catggtcgac ggcgctttcc tgcctgcaag ggctggacag cagcaacgtt attcgtccgt
    45601 tcacaccata cgtggtgatg cggatgggaa atgtcccgct ggtgccctat taccgaccgg
    45661 gcgataaacg catcgcacag gatctggcgg aactggcagc agacaatcag gcttttttac
    45721 tggcaaatca tggcccagtg gtttgcggtg aaagcctgca agaagccacc aacaatatgg
    45781 aagagctgga ggaaacggca aagctgattt ttattctcgg tgaccgcccg atccgttatc
    45841 tgaccgcagg tgaaattgcg gaattaagga gttaatgcaa tgcctcgttt tgcagctaat
    45901 ttatccatga tgttcaccga agtgcctttt attgaacgct tcgccgcagc gcgaaaagcc
    45961 gggttcgatg ctgtggaatt tctgtttccc tatgactact ccaccctgca aatccaaaag
    46021 caactggagc aaaaccatct gacactggcg ctgttcaata ccgctcccgg agatattaat
    46081 gcgggggaat ggggattatc cgcccttccc ggacgtgagc acgaagcaca cgcagacatc
    46141 gacctggcac tggaatatgc attggcgctc aattgtgaac aagtccatgt gatggcaggc
    46201 gtcgtgcccg ctggcgaaga tgctgaacgg taccgggcag tatttatcga taatctccgc
    46261 tacgctgccg accgctttgc gccacacggc aagcgaatat tagttgaagc actcagcccc
    46321 gacgttaagc ctcattatct cttctccagc cagtatcagg cactggctat tgttgaagag
    46381 gttgcgcgag ataacgtgtt tattcaacta gatacttttc atgcgcaaaa agtagatggc
    46441 aacctgacgc atttaatccg cgactacgct gggaaatatg cgcacgtaca aattgccgga
    46501 ctacctgatc gacatgaacc ggacgatgga gaaatcaact atccgtggct gttccgcctg
    46561 ttcgatgagg tgggatatca gggctggatc ggttgtgaat ataaacctcg tggcctcacc
    46621 gaagaagggc ttggctggtt tgacgcctgg cgctaattcg ttaaatccct aattacaacg
    46681 tacccataca tccccccatc tacggatggg gtaggttaac tataactttc agacagggtt
    46741 tcccatgtcc acaattacat tgttatgcat tgcgttagct ggcgtaatca tgctgttgct
    46801 gctggtcatc aaggcaaagg tacaaccatt cgttgctctg ctcctcgtca gcctgttagt
    46861 cgcacttgcg gcgggtatac cggcgggcga agtgggtaaa gtgatgatcg ccgggatggg
    46921 cggtgtgctt ggttccgtca ctattattat tggtctgggc gctatgctgg ggcgtatgat
    46981 cgaacactct ggtggtgcag agtcactggc taattatttc agtcgcaagt taggtgacaa
    47041 acgaactatc gctgcgctga ctctggcagt gttcttcctc ggtattcccg tcttctttga
    47101 tgtcagcttt attattcttg cgccaatcat ttacggtttt gccaaggttg ccaaaatatc
    47161 gccactcaaa tttggcctgc ctgtcgctgg aatcatgcta actgttcatg tggcagtacc
    47221 gccgcatcca ggccctgtcg ccacagcggg gttactccac gcagacatcg gctggctaac
    47281 catcatcggt attgcgattt ctattcccgt agggattgtt ggctactttg cagcgaaaat
    47341 aatcaataag cgccaatatg cgatgtcagt agaagtactg gaacagatgc aactggctcc
    47401 ggccagtgag gaaggcgcga caaaattaag cgataaaata aatccaccgg gcgtcgcgct
    47461 ggtcacctcg ctaattgtta ttcctatcgc gattatcatg acgggtacgg tttccgcaac
    47521 actgatgccg ccttcgcatc ccctgcttgg tacgctacag ctgatcggct caccaatggt
    47581 agctctaatg attgcgctgg tgctggcatt ctggttattg gctttacgtc gcggctggag
    47641 cttacaacac accagcgaca ttatgggctc agcccttccc actgcggcgg tagtgatttt
    47701 ggttactggt gctggagggg tatttggcaa agtgctggtg gaatcgggcg tcggcaaagc
    47761 ccttgccaat atgctacaaa tgattgacct gcctctgtta ccagccgcat ttattatttc
    47821 actggctctg cgtgcatcgc aggggtcagc aaccgtagca atcctgacaa ccggcgggtt
    47881 actctcagaa gcggtgatgg gattgaatcc gattcagtgc gtattggtga cgctggcagc
    47941 ctgctttggt gggcttggcg cgtcacatat taatgactca gggttctgga ttgtgaccaa
    48001 atatctgggg ttgtcggtag cagacggtct gaaaacctgg acagtgttaa cgaccattct
    48061 cggttttacc ggattcttaa ttacctggtg cgtatgggcg gcaatttgac cttaataaaa
    48121 aggtccgatg ggcatcggac cttttattgt gcacagaaaa ggccagcctc gcttgagact
    48181 ggcctttctg acagatgctt acttactcgc ggaacagcgc ttcgatattc agcccctgcg
    48241 tttgcaggat ttcacgcaaa cggcgcaggc cttcaacctg aatctggcga acacgttcac
    48301 gggtgaggcc aatttcacga cctacatctt ccagtgttgc cgcttcgtac cccagcaaac
    48361 cgaatcgacg tgccagcact tcacgctgtt tggcgttcag ctcgaacagc catttgacga
    48421 tgctctgctt catatcgtca tcttgcgtgg tatcttccgg accgttctct ttttcatcgg
    48481 ccaggatgtc cagcaacgct ttttcggaat caccacccag cggggtgtct accgaggtaa
    48541 tgcgctcgtt aagacgaagc atacggctga cgtcatcaac tggcttatcc agttgctctg
    48601 cgatctcttc cgcacttggt tcatggtcca gcttatggga caactcacgt gcggttcgca
    48661 ggtaaacgtt cagctccttt acgatgtgaa tcggcaaacg aatagtacgg gtttggttca
    48721 taatcgcccg ttcaatcgtc tggcgaatcc accaggttgc gtatgttgag aagcggaaac
    48781 cacgttccgg gtcaaacttc tctaccgcgc ggatcagccc caggttgccc tcttcgataa
    48841 ggtccagcaa cgccagacca cgattgccat aacggcgggc aatttttacc accagacgca
    48901 agttactctc gatcatccgg cggcgagagg cgacatctcc acgcagtgcg cgacgcgcaa
    48961 aataaacttc ttcttcggcc gttaacagtg gtgaataacc aatctcacca aggtaaagct
    49021 gagtcgcgtc caacacacgc tgtgtggctc cctgcgataa cagttcctct tcggccaaat
    49081 cgttatcact gggttcctct tctactaagg ccttttcgtc aaaaacctca actccgttct
    49141 catcaaattc cgcatcttca tttaaatcat gaactttcag cgtattctga ctcataaggt
    49201 ggctcctacc cgtgatccct tgacggaaca ttcaagcaaa agcctggttc cgccgattta
    49261 tcgctgcggc aaataacgca gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg
    49321 caagcgtgtt gaactggttc cggtgctacc catggttgct attttttgcc ccgccttcac
    49381 ttcttgttgt tcccggacca gcattgtgtc gttatgggcg taggcactca ggtaatcatc
    49441 attatgtttg atgataatca gattaccgta gccgcgcagc gcgttaccgg cataaacaac
    49501 gcggccatct gcggtcgcga taattgcctg tcctttgctg cctgcgatat caatcccctt
    49561 gttgcccccc tcagaagcgc caaaggtttc gatcactttg ccctcagtcg gccagcgcca
    49621 ggtggagata ggcgtactgg ttgatgtact gctgacagtt ggctcggttg tgcttgctgt
    49681 tggtaccgtt acaggcgctg tgaccgtggt cgcagctggc ttgttgttcg gcaacatttt
    49741 gttagcactc tgttcacccg aagattcaga atacgtaatt gtcggttgcg acgcaacagc
    49801 aacggtggaa ttttgtgcag gcttgatcac aactccttgc tctgctgcgt cggcctgggt
    49861 aatggcattt ccgccagtga ttggcgtacc ggaagcatta cccacctgca aggtctgacc
    49921 aacattcagc gcgtatggtg cctgaatatt gttgcgctga gcaaggtcac ggaaatcgtt
    49981 gccagtaatc caggcgatat agaaaagtgt gtcgcctttt ttcacggtat aggtactgcc
    50041 gctataactg cctttcggaa tgttcccata ctgacggtta tagacgatgc gtccgttttc
    50101 catctgtacc ggctgctgag ctactggctg cactggctgg atttgcggtt gctgggctgt
    50161 agacgtcgtc cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg
    50221 cgcattgcca ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc
    50281 cagccatagc gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc
    50341 cgcgctcatt tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa
    50401 ggcaccctac tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc
    50461 aggaaaaatc caggtatttc ctcacgtttt aagccagctc ccccttcact aaagggacaa
    50521 agcgcacggc ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca
    50581 aatactggtg ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca
    50641 ttagcgcagt tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcgc
    50701 gtgcctgcca accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat
    50761 ttttcaggcg gcgacgcgcc tgccactgca aacctttaat ccgttcgacc gagcaaacat
    50821 gctggacaag atgcgccagg attgccgttt gatatcccga accggtgcca atttccagca
    50881 cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg
    50941 aaattgtctg cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg
    51001 cttcatcaac gaatttttca cgcggcacgg cagcaagtgc attcagcacc tgctcatcct
    51061 gaataccttg cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt
    51121 gccaactccc acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa
    51181 atccacatgc agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc
    51241 cggaccggca tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg
    51301 atcttgctgc gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat
    51361 acctttgatt tgatccaagg gtaaatccgg aacgttaata ttaagaatac gtccggtgcg
    51421 cagcggctct ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta
    51481 atgtttatgc ccgtcaagcg agacggccag cgccggaaaa cctaaatgac ggccttccat
    51541 cgcggcggct accgtaccgg aataaataac gtcatccccc agattcggcc cggcgttaat
    51601 gccggacaca acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca
    51661 atcggtcggg gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag
    51721 ggaggattcc agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac
    51781 ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac
    51841 cccgtcatca ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga
    51901 caacgctggt tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat
    51961 cccaccaatt ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa
    52021 ctttttcgcg caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gcttgttcga
    52081 atgccagcgc ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca
    52141 tcagctcttt atcgttgacg cgatgctgta attccgccag ttcttcggtg gttgctacaa
    52201 accagctacc acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg
    52261 cttttttgag gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc
    52321 aaaaactccg tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc
    52381 cctgcaagtt gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt
    52441 ttacgcaaat atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa
    52501 ccagggtaaa ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat
    52561 actccagcac ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcctg
    52621 gcacgcgagc gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc
    52681 tgacttcacg ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat
    52741 tgcagccgtt tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc
    52801 ccaaatcttc caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg
    52861 gtttaccgtg gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag
    52921 cgccaccgct tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt
    52981 agtggctttc acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg
    53041 catttgtgga atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt
    53101 gcccagggta taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct
    53161 gtcggcacct ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc
    53221 cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc
    53281 cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc
    53341 aaaggcatgt acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt
    53401 cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc
    53461 gcacggcctt cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg
    53521 gtaatagtcg cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg
    53581 aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca
    53641 tttttgcccg gttcggcacg tttcatcgta tcgcgcactg gtgcggctag gatccctccc
    53701 gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga
    53761 cgagcggcgt catgcaccaa tacccactgc gcgtcgccag cggctttcaa acctgccagc
    53821 acggaatcgg cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga
    53881 ggaagttgtg caaaacggct atcgccagga cttatggcaa tgacgacacg tttcacccgg
    53941 ggatgcgcca gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga
    54001 tattgcttag gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg
    54061 caaacatcca aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc
    54121 cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc
    54181 ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga
    54241 tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc
    54301 tgtgccgcca catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac
    54361 agcgaatact gtagccagat cagaatagcc agcaacagca gcgttagttt acccatcctg
    54421 ccccctgaaa aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg
    54481 gggataccgc gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg
    54541 gcgtgcagaa cataatctca ttattagtta cggtttgaat tatgaacaga ggagacaaga
    54601 aagtacaaat tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac
    54661 cagggtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc
    54721 caccactacc gcgacaggca tcagtgccag aaagaaaggc caggtgtaga taaagaagaa
    54781 cagcgtgtta gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa
    54841 accgaccacg gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt
    54901 atttgttagt gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg
    54961 ggcatggaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta
    55021 ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt
    55081 caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt
    55141 tcttatataa gcctttggga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca
    55201 cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg
    55261 gcgagataaa tgcggtcagc accaccagtc cggcttcaac catcaaattc gccacttcac
    55321 cgacgcgacg gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc
    55381 cgtggcgaac attgtcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct
    55441 cctccagcac cccggcgacc gttgatttac cggacccaga gaggccggta aaccacagca
    55501 ctacaccacg atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc
    55561 agacgacgtt ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca
    55621 gtgcggaaag tggcgacgaa ccagggcatt caattccagt tcgaatgcac tgaattcaga
    55681 tgacgcaacg gcggcctcgt ttttcggctc atgcaccata ccggcaccaa cagtcacgtt
    55741 gctcaggcga tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa
    55801 caccagcggc tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc
    55861 aacttcacgc tgggtaaggt tattaatatc aacctgatag cgaatgccat caacacgcgc
    55921 gcgcgtcttc ttaccggcaa ttttgatgtc gtaactctgg cccggggaaa gcggctgttc
    55981 cgccatccat accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc
    56041 cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc
    56101 tccggcaaag gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc
    56161 cacaccagag ggcagcgctt tgacacgttg cccgacttcc acgcgaccgg atgccagcgt
    56221 tccggcgtag ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat
    56281 tggctgggca tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg
    56341 accgctgtac cacgacatac tttcactttg cgatgccacg ttgtcgcctt ccagtgcaga
    56401 gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaagg tcaaataatc
    56461 ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc
    56521 cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc
    56581 gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt
    56641 cgccatattg cgagtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt
    56701 ctcggtagag aaataacggt aagccacgtc aatggtgatg ccctgttcgc gctcagcttg
    56761 caggccgtcc accagcagtg ccagatccag cttttcgccc tgggtgccgt gacgcttact
    56821 gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg
    56881 accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct
    56941 tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc
    57001 aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct
    57061 ggtcgcggtc aatcacgcgg ccctgacgtt cactggtggt agaaaccagc atctcttcga
    57121 tgatctccgg cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca
    57181 gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt
    57241 tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat
    57301 acagtggcac aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc
    57361 agttagagag cgggaagacg cggatgcttt cacctttgtt aatttgcccg ttgtagttgt
    57421 gccacagctc cgggcgctga tttttcggag cccagcgatg gaagcggtca cggaaagagt
    57481 agattcgctc tttagcgcgg gatttctctt catcacggcg cgcaccaccg aaggcggcat
    57541 caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt
    57601 tcgcgctgcc gtgtacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca
    57661 ccagcagctc gcagccgtag gctttagcag tacgatcgcg gaactcatac atctcgcgga
    57721 atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct
    57781 tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat
    57841 ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca
    57901 ggtgagtaag tcgtttttga gccataaccg ttcctttgca ataccgctat tttcttgcca
    57961 tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt
    58021 agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg
    58081 atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg
    58141 cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt
    58201 accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa
    58261 cctggcgatt ttgctaatac tcaggcacga catattgcta ctttctttcc gggacgcatg
    58321 accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg
    58381 ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat
    58441 cgtaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagcg
    58501 ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac
    58561 gccgatgcca atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta
    58621 ggtgtcatgc tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga
    58681 tttgtggcga ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg
    58741 atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt
    58801 ggcgataaat tgtatttcaa cagcggtgta aaaaccactg aagtagtaag gaaattaacg
    58861 cgcgacaggg cgctggcaat tgcgcgcagt cacggaatag ccgcaacgac caatccgggg
    58921 ttgaataaaa attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa
    58981 gcgggcattg ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt
    59041 tatcagcaac gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga
    59101 ctggataatc aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt
    59161 gacgttatgc ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt
    59221 tgaaaatggg agctgagagt tctaccgcag gggcggggaa ttctaagtga tatccattat
    59281 cgcatccagt gcgcccggtt tatccccgct ggcgcgggga acacactaag catacataac
    59341 tgtttttaaa caaatttatt ccgcatcaac aatctaccaa ctaaattaaa acatttcctt
    59401 atttttaaag aacggataac ttattgatta tcaacaggaa gaaaagaaac caaacgtaac
    59461 ccatccaaat ccaccggaat acgtctgttt tctccccagg tctgaaattc aaaacccgac
    59521 tcggtattgg tcgcccaggc catcaccaca tttccgcaac cagccagttg ggtaatttgc
    59581 tgccagatca tctcccgaat acgttttgat gtatcaccaa catacacacc ggcacgcact
    59641 tccagtagcc agattgcgag ccgtccacgt aagcgcggcg ggacattttc tgtaacaacc
    59701 acgaccatgc tcatcagccg cgtcctcgat gaccgctatc gcccaacgtt tcaggttcgg
    59761 ggatggctgg cggtaacata tccggcgcag gttgtggcgg ttcaatttca cctgcggcaa
    59821 ggacttcctc aattaacggt attaatttgc ccgttaactt agtgctacgg aaaatatcac
    59881 gacaggctaa tctgacttct ttatcaggtt ctgcgggttg cctcgccgct atttcaaatg
    59941 cctttggtac aaccgaatca aatttaatga tatcggctat gtcataaaca aatgaaagcg
    60001 gtttgccact atggataaac ccaatagcgg gcgcatatcc cgcggctggt aatgact
//
LOCUS       DAJTMK010000004        54703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTMK010000004 DAJTMK010000000
VERSION     DAJTMK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54703
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.004"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..1123)
                     /locus_tag="OO135_000188"
                     /pseudo
     CDS             complement(1..1123)
                     /locus_tag="OO135_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1246..1698)
                     /gene="tabA"
                     /locus_tag="OO135_000189"
     CDS             complement(1246..1698)
                     /gene="tabA"
                     /locus_tag="OO135_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5396173.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            complement(1843..2436)
                     /locus_tag="OO135_000190"
     CDS             complement(1843..2436)
                     /locus_tag="OO135_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5396174.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            2507..3220
                     /gene="bdcA"
                     /locus_tag="OO135_000191"
     CDS             2507..3220
                     /gene="bdcA"
                     /locus_tag="OO135_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5396175.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3343..3735
                     /locus_tag="OO135_000192"
     CDS             3343..3735
                     /locus_tag="OO135_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5396176.1"
                     /translation="MTKSAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGTP
                     EPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAPP
                     YPTWTAVGVTWLAGFDFEIKVIERIPEL"
     gene            4015..4149
                     /gene="pyrL"
                     /locus_tag="OO135_000193"
     CDS             4015..4149
                     /gene="pyrL"
                     /locus_tag="OO135_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5396177.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4153..5088
                     /gene="pyrB"
                     /locus_tag="OO135_000194"
     CDS             4153..5088
                     /gene="pyrB"
                     /locus_tag="OO135_000194"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5396178.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5101..5562
                     /gene="pyrI"
                     /locus_tag="OO135_000195"
     CDS             5101..5562
                     /gene="pyrI"
                     /locus_tag="OO135_000195"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5396179.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5635..6021
                     /gene="ridA"
                     /locus_tag="OO135_000196"
     CDS             5635..6021
                     /gene="ridA"
                     /locus_tag="OO135_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5396180.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(6227..8923)
                     /gene="mgtA"
                     /locus_tag="OO135_000197"
     CDS             complement(6227..8923)
                     /gene="mgtA"
                     /locus_tag="OO135_000197"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5396181.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPACEGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9064..9117)
                     /gene="mgtL"
                     /locus_tag="OO135_000198"
     CDS             complement(9064..9117)
                     /gene="mgtL"
                     /locus_tag="OO135_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5396182.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9302..10267
                     /gene="treR"
                     /locus_tag="OO135_000199"
     CDS             9302..10267
                     /gene="treR"
                     /locus_tag="OO135_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5396183.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            10368..11789
                     /gene="treB"
                     /locus_tag="OO135_000200"
     CDS             10368..11789
                     /gene="treB"
                     /locus_tag="OO135_000200"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5396184.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYCIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            11839..13494
                     /gene="treC"
                     /locus_tag="OO135_000201"
     CDS             11839..13494
                     /gene="treC"
                     /locus_tag="OO135_000201"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5396185.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            13888..16026
                     /gene="nrdD"
                     /locus_tag="OO135_000202"
     CDS             13888..16026
                     /gene="nrdD"
                     /locus_tag="OO135_000202"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5396186.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKQLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16125..16589
                     /gene="nrdG"
                     /locus_tag="OO135_000203"
     CDS             16125..16589
                     /gene="nrdG"
                     /locus_tag="OO135_000203"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5396187.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16634..17020)
                     /gene="cybC"
                     /locus_tag="OO135_000204"
     CDS             complement(16634..17020)
                     /gene="cybC"
                     /locus_tag="OO135_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5396188.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17204..18556)
                     /gene="pmbA"
                     /locus_tag="OO135_000205"
     CDS             complement(17204..18556)
                     /gene="pmbA"
                     /locus_tag="OO135_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5396189.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18650..19201
                     /locus_tag="OO135_000206"
     CDS             18650..19201
                     /locus_tag="OO135_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5396190.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            complement(19284..20657)
                     /gene="mpl"
                     /locus_tag="OO135_000207"
     CDS             complement(19284..20657)
                     /gene="mpl"
                     /locus_tag="OO135_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5396191.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTSQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            20833..21831
                     /gene="fbp"
                     /locus_tag="OO135_000208"
     CDS             20833..21831
                     /gene="fbp"
                     /locus_tag="OO135_000208"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5396192.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(21864..22859)
                     /gene="yjfF"
                     /locus_tag="OO135_000209"
     CDS             complement(21864..22859)
                     /gene="yjfF"
                     /locus_tag="OO135_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5396193.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22846..23868)
                     /locus_tag="OO135_000210"
     CDS             complement(22846..23868)
                     /locus_tag="OO135_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5396194.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAVMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCALIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(23882..25384)
                     /locus_tag="OO135_000211"
                     /pseudo
     CDS             complement(23882..25384)
                     /locus_tag="OO135_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            complement(25524..26480)
                     /gene="ytfQ"
                     /locus_tag="OO135_000212"
     CDS             complement(25524..26480)
                     /gene="ytfQ"
                     /locus_tag="OO135_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR5396195.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            26790..27320
                     /gene="ppa"
                     /locus_tag="OO135_000213"
     CDS             26790..27320
                     /gene="ppa"
                     /locus_tag="OO135_000213"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5396196.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27700..28041)
                     /locus_tag="OO135_000214"
     CDS             complement(27700..28041)
                     /locus_tag="OO135_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5396197.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(28044..31823)
                     /gene="tamB"
                     /locus_tag="OO135_000215"
     CDS             complement(28044..31823)
                     /gene="tamB"
                     /locus_tag="OO135_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5396198.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFFR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLEVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(31820..33553)
                     /gene="tamA"
                     /locus_tag="OO135_000216"
     CDS             complement(31820..33553)
                     /gene="tamA"
                     /locus_tag="OO135_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5396199.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            33759..34397
                     /gene="msrA"
                     /locus_tag="OO135_000217"
     CDS             33759..34397
                     /gene="msrA"
                     /locus_tag="OO135_000217"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5396200.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHLMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            34720..36063
                     /locus_tag="OO135_000218"
     CDS             34720..36063
                     /locus_tag="OO135_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5396201.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36125..36331)
                     /locus_tag="OO135_000219"
     CDS             complement(36125..36331)
                     /locus_tag="OO135_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5396202.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            36656..37210
                     /locus_tag="OO135_000220"
     CDS             36656..37210
                     /locus_tag="OO135_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5396203.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(37300..38211)
                     /locus_tag="OO135_000221"
     CDS             complement(37300..38211)
                     /locus_tag="OO135_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396204.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDKFAIFIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
     gene            complement(38423..39163)
                     /gene="cysQ"
                     /locus_tag="OO135_000222"
     CDS             complement(38423..39163)
                     /gene="cysQ"
                     /locus_tag="OO135_000222"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5396205.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPRFRVSIY"
     gene            39353..41296
                     /gene="cpdB"
                     /locus_tag="OO135_000223"
     CDS             39353..41296
                     /gene="cpdB"
                     /locus_tag="OO135_000223"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5396206.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(41425..41805)
                     /locus_tag="OO135_000224"
     CDS             complement(41425..41805)
                     /locus_tag="OO135_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5396207.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41894..42754
                     /gene="qorB"
                     /locus_tag="OO135_000225"
     CDS             41894..42754
                     /gene="qorB"
                     /locus_tag="OO135_000225"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5396208.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVRFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            42862..43827
                     /locus_tag="OO135_000226"
     CDS             42862..43827
                     /locus_tag="OO135_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5396209.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43935..44597
                     /gene="ytfE"
                     /locus_tag="OO135_000227"
     CDS             43935..44597
                     /gene="ytfE"
                     /locus_tag="OO135_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5396210.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(44642..46054)
                     /gene="cycA"
                     /locus_tag="OO135_000228"
                     /gene_synonym="dagA"
     CDS             complement(44642..46054)
                     /gene="cycA"
                     /locus_tag="OO135_000228"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5396211.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(46363..46983)
                     /gene="fklB"
                     /locus_tag="OO135_000229"
     CDS             complement(46363..46983)
                     /gene="fklB"
                     /locus_tag="OO135_000229"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5396212.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            47202..47840
                     /gene="ytfB"
                     /locus_tag="OO135_000230"
                     /pseudo
     CDS             47202..47840
                     /gene="ytfB"
                     /locus_tag="OO135_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
     gene            complement(47824..48471)
                     /locus_tag="OO135_000231"
     CDS             complement(47824..48471)
                     /locus_tag="OO135_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR5396213.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            48683..49474
                     /locus_tag="OO135_000232"
     CDS             48683..49474
                     /locus_tag="OO135_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5396214.1"
                     /translation="MKSTRPVAVITGAARGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLECLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            49484..50326
                     /locus_tag="OO135_000233"
     CDS             49484..50326
                     /locus_tag="OO135_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5396215.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQSLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            50336..51112
                     /locus_tag="OO135_000234"
     CDS             50336..51112
                     /locus_tag="OO135_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCR5396216.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            51122..52663
                     /locus_tag="OO135_000235"
     CDS             51122..52663
                     /locus_tag="OO135_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR5396217.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGSGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYAAPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHRKQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGVPGVAPREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDITPDGLHIAQSGQIPKLVTKVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            52660..>54703
                     /locus_tag="OO135_000236"
     CDS             52660..>54703
                     /locus_tag="OO135_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR5396218.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVNYPLWQTDA
                     LVVGSGAARLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGLRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRSVEGDVAQLVASS
                     LNIVREVIAQALANENGMALVTVKDKIQARMSDYAGFICHADKVRHATRDALLLSEFV
                     QQHGLTINHAGEIAELFMWRHMTLTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSERAEDKNHRLTIQYSQGSFITEVKSLRMQPCINGIYFEKTGQT"
BASE COUNT        13501 a        14362 c        13594 g        13246 t
ORIGIN      
        1 atttttatag ggttctttat cgagattgaa aaataaggtc tcagctattt taattttaga
       61 ttgatcgcag ctaaaatcaa tatgatttat cacggagcat gcatacttta tcttttcaga
      121 atcaccaaaa tattgcattc ttttattaaa acaccaatcc agtattctta catcattaat
      181 atatggggat tttaaaaaag tctccagaat cgatgaccgg tgtaaggtta acttttgcat
      241 catctaagga atcaataaga atatgagaca ggttaacttt gatgtcatta atttcattgt
      301 cagtagcttt cttattatta agaagataat tatttactgc gttaaggtca tctatttctt
      361 tcagtatccc tttttcacca agcaagtatg atatcgattc attacataca taaggcatta
      421 atagcagatg ttctttcctt attgctaata atgcattatt caatggtttt tctaataaat
      481 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
      541 aattatttaa ctcctgcgaa atatttaata gacgagctat ttccttgtta gcttcgcgtt
      601 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      661 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      721 gtccttgacg agtccccata gtagtgaaag tggaaagtgt gatttctatt tccaccttag
      781 ataaatccga tcgttcccct tcaggattat tcataacaaa ctttattatc tcactgccat
      841 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgtttcgt
      901 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      961 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
     1021 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
     1081 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
     1141 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
     1201 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
     1261 cttcactact gctttgcgaa cccgggctgg cgcgcccact gcgcacagcg gtttatgcac
     1321 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
     1381 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gggtgcctgc
     1441 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1501 gcgggcatgg tactccgcac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1561 ataaaacaga cgattgcctt cgatatcgtg cttgcccttt ggcgtttctg ccgtaacgtg
     1621 tgctttgata tgctcaatcg cctggcgtaa ctcctggggt agccacggct gaagattatg
     1681 aatatttccg atgatcatct tttccactcc ttaattaaaa caacgtttca tttccatcgt
     1741 tatacgaaaa gatgatcaat tccgctagcc ttaacgcgtg aattattgtc ttatcacaac
     1801 ttatcggcag gcgcgttccc gcctttcggg tcaaggcccg catcactcct tgagaatagt
     1861 ttttatcgct tcccccgcca gtgcagctgt tgcacagagt tgttcgatgg agtgcccctc
     1921 ccgtgccttc gcagaaagcc ctgacatcac ggtactcata aaatcagtca cacattgtgc
     1981 gctttgtgga tgccgcctgg cgatatagtc ataaatggtc gtttctgcgg cgtgataata
     2041 ttgaacggca atatcacgcg cttgtggatc atgactatga atgccttcaa gaaccataca
     2101 gccagcgcag ccgccgtttt ggctatatct gcgcgccgct tcttttaata cctcagccag
     2161 gcactcccct accggacgat catcacgaag aatatcggca agcggaatag cttccgtacc
     2221 gacatattca ttgagtacac ggctaaataa cccagcctta ttgccaaaag ccgcgtagag
     2281 gctcgggggg ttaataccaa gataatcagt aacttcagca acactgacag catcgaaacc
     2341 tttttggtga aaaagcactt ttgccgcaga gactgcctgc tcaggagcga aacgtctggg
     2401 acgacctgga acgcgagatt gttttttagt gaccattaca aaacttgttg acagaaagtt
     2461 aaaacagttt tgtaatgcat gttacataat aaatcaagga gtccttatgg gcgcttttac
     2521 aggtaagaca gttctcatcc tcggtggtag ccgtggaatc ggtgccgcta tcgtacgtcg
     2581 tttcgtcacc gatggggcca atgtacgatt cacctatgcg gggtcgaaag atgccgctga
     2641 acgcctggca caagagactg gagcgacagc agtattcaca gatagtgctg acaaagacgc
     2701 tgttattgat gtcgttcgta agagcggcgc attggatatc ctggtggtaa atgcaggtat
     2761 tggcgtcttt ggcgatgccc tggaattaaa tgccgacgat attgatcgcc ttttcaaaat
     2821 caatattcat gctccttacc atgcctctgt tgaagccgcc cggcagatgc ccgaaggcgg
     2881 acgcatctta atcatcggct ccgtgaatgg cgatcgtatg cctgttgcag gcatggctgc
     2941 ttatgccgcc agtaaatctg ccctgcaagg catggcgcgc gggctggccc gtgatttcgg
     3001 accgcgtggg atcaccatca acgtcgtcca gccagggcca attgataccg acgctaatcc
     3061 cgccaacggg ccaatgcgcg atatgtcgca tagttttatg gccatcaaaa gacatgggca
     3121 accggaagag gtcgctggta tggtcgcatg gttagcaggg ccagaagcca gttttgttac
     3181 cggcgcgatg cataccattg atggcgcgtt tggcgcataa cagactacgc tcaattaagc
     3241 ccagccattt cccatgatgt ctgggttttg tttactcacg tcgtccgcta aaagcggctc
     3301 ctggtaaata taaatcttct gttcatgtcg taaatcttga ggataaccaa atccgctgtt
     3361 ttccctgctg gacgacattc actgtatgct gagcatcgtt attctgcggc tattcgttcc
     3421 ggcgatttgc tgtttgtttc cgggcaagta ggaagtcgag aggacggaac accagaaccc
     3481 gattttcagc aacaagtcag actggccttt gataatttgc atgcgaccct ggcagctgcg
     3541 ggatgcactt ttgacgatat cattgatgtt acgagctttc ataccgatcc agaaaaacaa
     3601 tttgaagaca tcatgacggt gaaaaatgaa atatttagcg cgccacctta tccaacctgg
     3661 acggcggtgg gcgttacatg gctggcaggc tttgattttg aaattaaagt gatagagcgc
     3721 atccctgagc tctaagcaat agtgctagcc gttcgttttc acactccgcc ctataagtcg
     3781 gatgaatgga ataaaatgca tatctgattg cgtgaaagtg aaaaagaaaa aagcaggaaa
     3841 tgtctgcaat tattgatacc gaacgacagt tccccccaga atcacagtaa ataaaaatgc
     3901 atatatctta actttaaatt caaataaacc gtttgcgctg acaaaatatt gcatcaattg
     3961 cttgcaccgc ttctgacgat gagtataatg ccggacaatt tgccgggagg atgtatggtt
     4021 cagtgtgttc gacattttgt cttaccgcgt ctgaaaaaag acgctggcct gccgtttttc
     4081 ttcccgttga tcacccattc ccagcccctc aatcgagggg cttttttttg cccaggcgtc
     4141 aggagataaa agatggctaa tccgctatat cagaaacata tcatttccat aaacgacctt
     4201 agtcgcgatg accttaatct ggtgctggca acagcggcaa aactgaaagc aaacccgcaa
     4261 ccagagctgt tgaagcacaa agtcattgcc agctgcttct tcgaagcctc tacccgtacc
     4321 cgcctctctt tcgaaacttc catgcaccgc ctgggtgcca gcgtggtggg cttctccgac
     4381 agcgccaata catcactggg taaaaagggc gaaacgctgg ccgataccat ttcggttatc
     4441 agcacttacg tcgatgcgat agtgatgcgt catccgcagg aaggtgcggc gcgcctggcc
     4501 accgagtttt ccggcaatgt accggtactg aatgccggtg atggctccaa ccaacatccg
     4561 acgcaaacct tgctggactt attcactatt caggaaaccc aggggcgtct ggacaatctc
     4621 cacgtcgcaa tggttggtga cctgaaatat ggccgcaccg ttcactccct gactcaggcg
     4681 ttagcgaagt tcgacggcaa ccgtttttac ttcatcgcgc cggacgcgct ggcaatgccg
     4741 caatacattc tggatatgct cgatgaaaaa gggatcgcat ggagtctgca cagctctatt
     4801 gaagaagtga tggcggaagt agacatcctg tatatgaccc gcgtgcaaaa agagcgcctg
     4861 gacccatccg aatacgccaa cgtgaaagcg cagtttgttc ttcgcgccag cgatctccac
     4921 aacgccaaag ccaatatgaa agtactgcat ccgctgccgc gtgttgatga aattgcgacg
     4981 gatgttgata aaacgccaca cgcctggtac ttccagcagg caggcaacgg gattttcgct
     5041 cgccaggcgt tactggcact ggttctgaat cgcgatctgg tactgtaagg ggaaatagag
     5101 atgacacacg ataataaatt gcaggttgaa gctattaaac gcggcacggt aattgaccat
     5161 atccccgccc agatcggttt taagctgttg agtctgttca agctgaccga aacggatcag
     5221 cgcatcacca ttggtctgaa cctgccttct ggcgagatgg gccgcaaaga tctgatcaaa
     5281 atcgaaaata cctttttgag tgaagatcag gtagatcaac tggcattgta tgcgccgcaa
     5341 gccacggtta accgtatcga caactatgaa gtggtgggta aatcgcgccc cagtctgcca
     5401 gagcgcatcg acaatgtgtt ggtctgcccg aacagcaact gtatcagcca tgccgaaccg
     5461 gtttcatcca gctttgccgt gcgaaaacgc gccaatgaca tcgcgctcaa atgcaaatac
     5521 tgtgaaaaag agttttccca taatgtggtg ctggccaatt aattgcggtt ggtaataaaa
     5581 gtctggctcc ctataatgag ccagactttt taccgctgta ataaaggaga aatcatgagc
     5641 aaaactatcg cgacggaaaa tgcaccagca gctataggtc cttacgtaca gggcgttgat
     5701 ctgggaaata tgatcatcac ctccggtcag atcccggtaa atccgaaaac gggcgaagta
     5761 ccggcagacg tcgctgcaca ggcacgtcag tcgctggata acgtaaaagc gatcgtcgaa
     5821 gccgctggtc tgaaagtggg cgacatcgtt aaaactaccg tgtttgtaaa agatctgaac
     5881 gacttcgcaa ccgtaaacgc cacttacgaa gccttcttca ccgaacacaa cgccaccttc
     5941 ccggcacgtt cttgcgttga agttgcccgt ctgccgaaag acgtaaagat tgagatcgaa
     6001 gcgatcgcta ttcgtcgcta atcttgatgg aaatccgggc tatcatgccc ggattaagtc
     6061 tgatgacaaa cgcaaaatcg cctgatgcgc tacgcttatc aggcctacgt gattcctgca
     6121 attgattgaa tttgtaggcc ggattaggcg tttacgccgc atccggcatg aacaaaactc
     6181 actttgtctg caatctgaat ccgggccacc atgcccagtt tattctttac tgccagccgt
     6241 aacgacggct ataaaatcct ttcaccaact gggttaacgt catataccct gccagaatcg
     6301 caaccagcca cgggaaatag cttaacggca acgcctgtaa ttgcagataa ctggccagcg
     6361 gtgaaaacgg caatgcgatc ccgacaatca tcacgatcac ggtcatgatc attaacggcc
     6421 acgatgcaca gctctgaata aacggcacac ggcgggtgcg gatcatatgc acaatcagcg
     6481 tttgcgacag taagcccacc acaaaccatc ccgactggaa cagcgtttgc gtttccggcg
     6541 tgttggcatg gaatacccac cacatcaggc aaaacgtcag aatatcgaag atcgagctga
     6601 tcggtccgaa gaagatcata aagcgcccca gatccgccgg attccaacgc tgcggctttt
     6661 gaatttgctc gtcgtcgacg ttatcaaacg ggatcgccac ctgtgacaca tcgtacagca
     6721 ggttctgaat aagcaagtgt aacggcaaca tcggcaggaa gggcaagaaa gcactcgcca
     6781 ccagcacgct gaacacatta ccaaagttag aactcgccgt cattttgatg tatttcagca
     6841 tgttggcgaa agtgcgacgt ccctcaataa ccccctcttc cagcaccatc aggctttttt
     6901 ccagcaggat gatatcagcc gcttcacggg caatatctac cgcgccgtcc acagaaatgc
     6961 cgatatccgc cgcgcgtaag gcgggcgcat cattaatacc atcgcccata aagccaacca
     7021 catgcccttc gcgcttcagt aaggtcacaa tgcgttcttt atgcatcggc gtcaggcggg
     7081 caaacagcgt ggtgcgctgt gcaagatttg ccagttcgtc gtcagatagc gtttcaatat
     7141 cactaccaat gaccacctct cccgcgtcca gccccacttc atggcacact ttcgctgcaa
     7201 ctaactcact gtcgccagta aggattttta cggtaattcc actcgctttt aatgccttca
     7261 gtgccggagc ggttgtctct tttggcggat caagaaaagc aatatatcct tcgaggatca
     7321 ggtcggattc atccgcccgc tggtaatctc cttcacacgc tggcaggtat ttcgtcgcca
     7381 ccgcaaccac gcgcagcccc tgacgattca gcgtatcagt aacccgctta atcttacgca
     7441 gcatgatgtc atcgagcggc acaatctcgc cattgtgacg cacctgcgaa cacacattga
     7501 ggatttcctg caatgcacct ttgcaaacca gctgatggtg ctcggtattt tctgccacta
     7561 ccaccgacat ccggcgacgc tcgaaatcaa acggaatctc atcgattttt tgccaacgac
     7621 tggccagcga gcgcgctgac tcttcatccg taccttcgag caccgctgta tcgagcaggt
     7681 ttttaagtcc ggtctgataa tgactgttca accacgcgct atgcagcacg cgttcgctgg
     7741 ttttaccgga gatatcggta tgattctcca gcacaatttt atcctgcgtc agggtgccgg
     7801 ttttatcagt gcacagaata tccattgcgc caaagttctg aatagcatcc agatgtttga
     7861 cgatcacttt ctgtttcgac agctttactg cgccgcgcgc cagagtcgag gtaacaatca
     7921 tcggcaacat ttccggcgtt aggcctaccg cgaccgaaag cgcaaacagc gccgcttccc
     7981 accagtcgcc tttggtgtag ccattgatta acagcaccac cggtgccatc accagcataa
     8041 agcgaatcag cagcatactg acgcggctga tcccttgctg aaaggcattc ggctcgcttt
     8101 cctgctcact aacacgcccc gccagttgac caaaccaggt attggcacct gtagcaatca
     8161 ccattgcttg tgccgtgccg ctcaccacgg tggtgcccat aaaacacagc gtgtcgcact
     8221 ccagcggatt gctgtgctcc ggctggcgag tggtagcggc tttttccacg ggcagagact
     8281 caccggttaa cgacgcctga gcgacgaaca gatcccgcgc ttgcaagata cgtaaatctg
     8341 ccgggatcat atcccccgcc gccagtttta taatatcgcc gggcaccagc tggtcgatcg
     8401 ggatctccag ccagccattt tcgcctttgt cgttaattac gcgcagcacc gtcgcggtat
     8461 tgctgaccat cgctttcagg gcatctgccg ctttagtgga acgtgcttcc tgaataaagt
     8521 tcagcaacgt agaaatagcg accattagcg cgataacgcc cgcagcaaat aaatcttccg
     8581 tggcgtaaga aatagcgccg agaatggtga gtaaaatatt aaaggggttg cgatagcaga
     8641 cccataaatg tacccaccac ggcgacagtt gttgtgcggg taatttattt tcaccatgtt
     8701 gttcgcgggc agattccact tccgcctgat ttaacccttc cggatgggtg tcgaacgttt
     8761 tccacaattc ttcttcgggc atcaccgcca ttttcaggca atgcgcactt aaggacggcg
     8821 ggaccaccgt attcactgtc tgctgcgcgc caggcaatgg atcacgatga accagacggg
     8881 aaggtaaatg gcgaatgagc cgggtaaaaa tttctttaaa cataaggagt ccctccgcac
     8941 tgtctgaaac agtacagaaa tgtcttcagg cgtggcgaag ccttacctga aggtaataaa
     9001 gctgagcagg gacgttaaaa cgtcacggcc ttacgcatcc ggtaacgccg tgaaaagacg
     9061 ggcttaccgg aaaagtttta atctccgtcg agggagaggc gtgggatcag gttccatata
     9121 acctccggta agtgaaaaaa gcgccgcaaa taataaggag aatccaataa tattcgtagc
     9181 atctatggcg atataatacc tgctggcaac taaaccaacg ataaaccaga ctttacccat
     9241 tgctgaatgc acgggtaaag ttaggctcaa ataattaaac aacacgttac aggacaacag
     9301 gatgcaaaat cggctgacca tcaaagacat cgcgcgctta agcggcgtgg ggaaatctac
     9361 agtttcccgg gtgctgaata acgaaagcgg cgtgagccag cgcacccgcg agcgtgttga
     9421 agcagtgatg aatcagcatg gattttcccc ttcccgctct gcgcgcgcta tgcgtgggca
     9481 aagcgataaa gtggtcgcca tcattgttac ccgcctggat tcgttgtcag aaaatctcgc
     9541 cgttcaaacc atgctgccag agttctatga acaaggttac gacccaatca tgatggaaag
     9601 tcagttttcc ccgcaattag ttgccgaaca tttgggggtg ctgaaacggc gtaatatcga
     9661 cggcgtagtg ctgttcggtt ttaccggcat aacagaagaa atgttagccc actggcagtc
     9721 atcgctggtt ctgctggcgc gtgacgcaaa aggctttgct tcggtctgtt atgacgacga
     9781 aggggcaatc aagatcctga tgcaacggct gtatgaccag gggcatcgta atatcagtta
     9841 tctcggcgtg ccgcacagtg acgtgacgac cggtaagcga cgtcacgaag cctacctggc
     9901 gttctgcaaa gcgcataaac tgcatcccgt tgccgctctg ccaggccttg ctatgaagca
     9961 aggctatgag aacgtagcaa aagtgattac gcctgaaact accgccttac tgtgcgcaac
    10021 cgacacgctg gcacttggcg caagtaaata cctgcaagag caacgcatcg acaccttgca
    10081 actggcgagc gtcggtaata cgccgttaat gaaattcctc catccggaga tcgtcaccgt
    10141 agatcccggt tacgccgaag ctggacgcca ggcggcttgc cagttgatcg cacaggtaac
    10201 cgggcgcagc gaaccgcaac aaatcatcat ccccgccacc ctgtccagat cgtttcctga
    10261 acgataaatt gtgatcttcg ctgcgtttcg ggaacgttcc cgtttttaaa tttttccgcg
    10321 caatatattc tgcagccaac caaaaatgtc atctgccatg gggctttatg atgagcaaaa
    10381 taaaccaaac ggatatcgat cggttgattg aactggtcgg cgggcgcggc aatattgcga
    10441 cggtgagcca ctgtattact cgcctacgct ttgtcctcaa ccaaccggcc aatgccagac
    10501 cgaaagaaat tgagcaactc cccatggtga aaggctgttt caccaatgcc gggcaatttc
    10561 aggtggtgat tggcaccaac gtgggtgact actatcaagc actgatagcg tcaaccggac
    10621 aggcgcaggt tgataaagag caggtaaaaa aagccgcccg gcagaatatg aaatggcatg
    10681 agcagttgat ctctcatttc gcagagacct tcttcccgtt gctgcccgcg ttgattagcg
    10741 gcggtttgat cctcggtttt cgcaatgtga tcggcgattt gcccatgagc aacggtcaaa
    10801 cgctggcgca aatgtaccct tccctgcaaa cgatctacga ttttctgtgg ttgatcggtg
    10861 aagcgatctt cttctacctg ccggtcggga tttgctggtc agcggtgaaa aaaatgggcg
    10921 gcacaccgat ccttggtatc gtgcttggcg tgacactggt ttccccccaa ctgatgaacg
    10981 cttatctgct cgggcagcag ctgccggaag tgtgggactt tggcatgttc agcatcgcca
    11041 aagtgggcta tcaggcgcag gtgatcccgg cactgttagc cgggctggcg ctgggcgtta
    11101 ttgaaactcg ccttaaacgc atcgtgccag attacctcta tctggtggtg gtgcccgtct
    11161 gttcgctgat cctcgcggtg ttcctcgccc atgcgctgat tggtccgttt ggtcgcatga
    11221 ttggcgatgg cgttgccttt gcggtacgtc acctgatgac cggcagcttt gctccgattg
    11281 gcgcggcatt gtttggcttc ctgtacgccc cgctggtgat caccggggta caccagacca
    11341 cgcttgctat tgatttgcag atgattcaaa gcatgggtgg tacgccagtg tggccactga
    11401 ttgcgctgtc gaatatcgct cagggctccg ccgtgatcgg cattatcatt tccagccgca
    11461 agcacaatga acgcgagatc tccgtgcctg cggcgatctc cgcctggctt ggggtcactg
    11521 agcctgcaat gtactgcatc aacctgaaat atcgcttccc gatgctgtgc gcgatgattg
    11581 gttctggtct ggctggattg ctatgcggcc tgaacggcgt tatggcgaat ggcatcggcg
    11641 taggcggcct gccgggaatt ctctcgattc aaccgaacta ctggcaggtg tttgcgctgg
    11701 caatggctat cgccatcatc atcccgattg tactcacctc gtttatctat cagcggaaat
    11761 accgcctggg cacgctggac attgtttaat tttcttcggg gcgcaattgc gctccctcgc
    11821 atttgcagga ataacgtaat gactaatcct ccccactggt ggcaaaacgg cgttatctac
    11881 cagatttatc caaagagttt tcaggacacc acgggtagcg gtaccggcga tttacgtggc
    11941 gttatccaac gcctggacta tctgcataaa ctgggcgttg atgccatctg gctaaccccc
    12001 ttttatgtct ctccccaggt cgataacggt tacgacgtag cgaactatac ggcgattgat
    12061 cccacctacg gcacgctgga cgattttgac gaactggtga cgcaggcaaa atcgcgcggg
    12121 attcgtatca ttctcgatat ggtgtttaac catacctcta cccaacatgc ctggtttcgc
    12181 gaggcgctga acaaagaaag cccttaccgc cagttttata tctggcgcga tggagaacca
    12241 gaaacgccac cgaacaactg gcgttcaaaa tttggcggta gtgcgtggcg ctggcatgcg
    12301 gaaagcgaac agtactattt gcatctcttt gcaccagaac aggcggatct caactgggag
    12361 aatccagcgg tacgcgcagt gctgaaaaaa gtctgtgagt tctgggccga tcgtggggtc
    12421 gacgggttgc gcctggatgt ggtgaatctg atctccaaag acccgcgttt ccctgatgac
    12481 ctggatggcg acgggcgtcg cttctacacc gacgggccac gagcacacga gtttttgcac
    12541 gagatgaacc gcgatgtgtt tacgccacgc gggttaatga ccgtaggtga aatgtcctcc
    12601 accagccttg agcattgcca gcgatacgcg gcactgacag gcagtgaatt gtcgatgacc
    12661 tttaattttc atcacctgaa ggtcgattat cccggtggtg aaaaatggac gctggctaaa
    12721 cctgactttg tggcgttgaa aacattgttc cgccactggc aacaaggaat gcacaacgta
    12781 gcatggaatg ccttgttctg gtgtaaccac gatcagccgc gcattgtttc tcgctttggt
    12841 gatgaaggtg aataccgcgt gcctgcggca aaaatgctgg cgatggtgct gcatggcatg
    12901 cagggaacgc cgtatatcta ccagggcgaa gagattggca tgaccaaccc gcatttcacg
    12961 cgcattactg actatcgcga cgtagagagc ctcaatatgt ttgccgagct gcgcaacgat
    13021 ggtcgtgatg ccgacgagtt attggcaatc ctcgccagta aatcccgtga caacagccgc
    13081 acgcccatgc aatggagcaa cggcgataat gccggattta cggctggcga accgtggatt
    13141 ggcctgggtg ataactatca acaaatcaac gtagaagccg cgctggccga tgattcctcg
    13201 gtgttttaca cctaccaaaa gttaatcgca ctgcgtaagc aggaagccat cctgacatgg
    13261 ggcaattacc aggatctgct gccaaacagc cctgtattgt ggtgctatcg ccgtgaatgg
    13321 aaggggcaaa ccttgctggt cattgccaac cttagccgtg agatccaacc ctggcagcca
    13381 gggcaaatgc gcggcaactg gcagcttgtg atgcataact acgaagaagc ctcaccacaa
    13441 ccctgtgcca tgaatttacg gccttttgag gctgtctggt ggttacagaa gtaaatcttc
    13501 cctaagcccc ggtaatgccg gtcattccgg ggttttgtct gtcagcatgt tgtttttgtt
    13561 tatttttcaa ccagcaaatt cattaaaaaa tttacataac gctgtagcgc ccgtcatccg
    13621 tacactctgc tttttacttt gagctacatc aaaaaaagtt caaacatcct tgatgcaaag
    13681 cactatatat agactttaaa atgcgcccca acccaatatg ttgtattaat cgactataat
    13741 tgctactaca gctccccacg aaaaaggtgc ggcgttgtgg aaaagcggat ggcgattgcg
    13801 gaaagcaccg gaaaacgaaa cgaaaaaacc ggaaaacgcc tttcccaatt tctgtggata
    13861 acctgttctt aaaaatatgg agcgatcatg acaccgcatg tgatgaaacg agacggctgc
    13921 aaagtgccgt ttaaatcaga gcgcatcaaa gaagcgattc tgcgtgcagc taaagcagcg
    13981 gaagtcgatg atgccgatta ttgcgccact gttgctgcag ttgtcagcga gcagatgcag
    14041 ggccgcaacc aggtggatat caatgagatc cagaccgctg ttgaaaacca gctgatgtca
    14101 ggtccataca aacaactggc tcgtgcttac atcgagtacc gtcacgatcg cgacattgaa
    14161 cgtgaaaaac gcggtcgcct gaatcaggag atccgtggtc tggtcgagca gaccaacgcc
    14221 tcgttactca acgaaaacgc caacaaagac agcaaggtga ttccaaccca gcgcgacctg
    14281 ctggccggga tcgtggctaa acactatgca cgtcagcacc tgctgccgcg tgacgtggtg
    14341 caggcacatg agcgtggcga tattcactat cacgatctcg attactcgcc gttcttcccg
    14401 atgttcaact gcatgttgat cgacctgaaa ggcatgctga cccagggctt taagatgggg
    14461 aacgccgaga ttgaaccgcc gaagtcgatc tctacggcaa ccgctgtaac tgcgcagatt
    14521 attgctcagg tcgccagcca tatttatggc ggcaccacca ttaaccgtat cgatgaagtg
    14581 ctggcaccgt ttgtcaccgc cagctacaac aaacatcgca agaccgcaga agagtggaac
    14641 atcccggacg ccgaaggcta cgctaactct cgtaccatta aagagtgcta cgatgccttc
    14701 cagtcgctgg agtacgaagt aaacaccctg cacaccgcca acggtcagac gccgtttgta
    14761 acctttggtt ttggtctggg caccagctgg gagtcgcgcc tgattcagga atctatcctg
    14821 cgtaaccgta tcgcaggcct cggtaaaaac cgtaaaactg cggtgttccc gaaactggtg
    14881 tttgcgattc gcgatggcct gaaccataaa aaaggcgatc cgaactacga catcaaacag
    14941 ctggcgctgg agtgcgcaag caagcgcatg tatccggata tcctgaacta cgatcaggta
    15001 gtgaaagtca ccggttcgtt taaaactccg atgggctgcc gcagcttcct cggcgtgtgg
    15061 gaaaatgaaa acggcgagca gatccacgat ggtcgtaaca acctcggcgt gatcagcctg
    15121 aacctgccgc gtattgctct ggaagcaaaa ggcgatgaag ccaccttctg gaagcagctg
    15181 gatgaacgtc tggtgctggc acgtaaggcg ctgatgaccc gtatcgctcg tctcgaaggc
    15241 gtgaaagcgc gcgtggcccc gatcctctat atggaaggtg cttgtggcgt gcgtctcaat
    15301 gctgacgatg atgtttctga aatcttcaaa aacggtcgtg cgtctatttc gctgggttac
    15361 atcggcatcc acgaaaccat taacgcgctg ttcggcggcg agcatgttta cgacaacgag
    15421 cagcttcgcg cgaaaggtat cgccattgtt gaacgtctgc gtcaggcagt ggatcagtgg
    15481 aaggaagaaa cgggttatgg tttcagtctc tacagcacgc cgagtgaaaa cctgtgcgac
    15541 cgcttctgcc gtctcgatac tgctgagttt ggcgtggtgc cgggcgtgac cgataaaggt
    15601 tactacacca acagtttcca cctcgatgtg gagaagaagg tgaacccgta cgacaagatc
    15661 gactttgaag cgccttaccc gccgctggcg aacggtggtt tcatttgcta cggcgagtat
    15721 ccaaacattc agcacaacct gaaggcgctg gaagatgtct gggattacag ctatcagcat
    15781 gtaccgtatt acggcaccaa tacaccgatt gatgagtgct acgagtgtgg ctttaccggt
    15841 gagttcgagt gcaccagcaa aggcttcacc tgcccgaaat gtggtaacca tgacgcctcc
    15901 cgtgtgtcgg taactcgccg cgtgtgcgga tatttaggta gcccggatgc acgtccgttt
    15961 aacgccggca agcaggaaga agttaagcgc cgcgttaaac atttgggaaa tgggcagata
    16021 ggttgagctg cactaagcaa atgccggatg tgatgtgaaa gcgtcgcatc ccgcgttgat
    16081 tgccggatgc agtctacacg cggactgata agccaggcga aataatgaat tatcatcagt
    16141 actatcctgt cgacatcgtc aacggccccg gcactcgttg caccctgttt gtctccgggt
    16201 gtgttcatga atgccccggt tgctataaca aaagcacatg gcgggtaaat tccggtcagc
    16261 catttactaa agcaatggaa gaccagatca ttaacgatct gaatgacact cgtatcaaac
    16321 gccagggtat ttccctctcc ggcggcgatc cgctgcatcc gcaaaacgtg ccggatattc
    16381 tgaaactggt acaacgcatc cgcgccgagt gtccgggtaa agacatctgg gtgtggacag
    16441 gctataaact cgacgaactc aacgcagcgc aaatgcaggt tgttgatttg attaacgtgc
    16501 tggtcgacgg caaatttgtg caggatttaa aagacccttc cctgatctgg cgcggcagca
    16561 gcaatcaggt ggtgcatcat ttgcgatgat aagttccctg ttacctgagt gcaggcaaca
    16621 gggaaatgag gaattaacga tacttctggt gataggcgtt gcgggtcgtt ttcagttgct
    16681 ctgcggcagc ctgcgcttct tttactttac cttcatttgc cagcttcagc gcgtcgtcaa
    16741 tctgaccgac cagaatatcg aaaccgtgac ggaagtcttt catctccggg ctgtccggtg
    16801 atttgccttc gagcttcggc ggcgttgctt tttgcgcatc cagcgctgcg gcgcgcatct
    16861 tcgttaacgc gtctttgact tgcgccgcgt tatcggcttt ttcgaccact tttaaattgt
    16921 cgttgagagt ttccatattg tcttcaagat cagcggcaaa cgacgccgaa ctgaatacca
    16981 acgaggagac tgcaagaata gctaacaggc tttttcgcat tgctcacttc cttttttatt
    17041 tacacttcat cattcacact gcctcatcga cgactacgct cattcgaccg ggtcacgtac
    17101 tgcgagtacg ctccccggcc ctcattcact ttccgtctcg atgcagtgcg aatgattttg
    17161 tgtaattttt tagtttatag cgcgacaggt cgcgccagtt tttttactgt ccggcgattt
    17221 tcatctccgg caacagcaca gaaccacact gtatattact gcgtgtttca atatcgttac
    17281 cgacggtgac aatattgcgc cacatatctt ttaaattacc tgcgatggtg atttcgctaa
    17341 ccggatactg aatttcgccg ttttctaccc agaagcccgc cgcaccacgg gaataatcac
    17401 cggtaattgc actcacgccc tggcccatca attccgtcac caccagcccg gtgcccatct
    17461 ctttgagcat ctgctcgaag cttagacctt gtccggcaat ccgccagttg tgaataccgc
    17521 ccgcatgtcc ggtgcttttc agccccagtt ttcgcgccga atagctggtc agcagccact
    17581 gagtcaggat gccatcttta ataatatcgc gacgctcggt gcgcacacct tcgctgtcga
    17641 atggcgtcga cgccagccct ttcagcagat gcggatgctc ttcaatggtc agccagtccg
    17701 gcagaatttg tttacccagc gaatccagca ggaaggtaga tttacggtaa accgatccac
    17761 ccgctatcgc ccccaccaga tggccaaaaa gcccggttgc cacttcattg gcaaaaatga
    17821 ccggcgcttt catggtggag agtttacgcg gtgacaggcg cgataaagta cggcgagcac
    17881 agtcggcccc aacccactct ggcgtttgca gatcgctcat cgcacgacca atggtgtagg
    17941 cgtaatcacg ctccatatcg ccattttctt cggcaattac acagctggag agcgaatgac
    18001 gcgttgagca gtaaccctgc aacatgccgt ggctgttgcc aaaaactttg acaccgtagt
    18061 ggctgttaaa gctgccacct tcggtattgg tgatgcgttt gtccgcctgc aatgccgcct
    18121 gttctgcgcg ggccgccagt tcaatggctt catccgggga aacttccgca gggtggaaca
    18181 agtcgagatc tggtgcgtca aaggccagca gctctttgtc tgccacgccg gcacagggat
    18241 ctggcgaggt ataacgggca atatccagcg ccgcctgtac agtacgggca atggcctgcg
    18301 ggcttaaatc ggtggatgat gcgctacctt tgcggttctg gtgataaaca gtgatcccca
    18361 gcgcgccatc gctattgaat tcaacattct ccacttcacc ataacgcgtg cttacgctaa
    18421 tgccggtggt cttgctgacg gcaacttccg caccgtccga tttgcctgag gccaactcca
    18481 gcgcagtgga aactgcttct tccagaatct tgcgctgcgc ttcaacttga gagattactt
    18541 tcattgcaag tgccataatg tagagagttt ctctgaagtc taacagagaa ccgtttttca
    18601 gtgcgcatct taactggtaa cattagcctc tttttttaag gagcctgaga tgactaagca
    18661 gcccgaagac tggctcgacg acgttcccgg tgatgacatc gaagacgaag acgatgaaat
    18721 tatctgggtc agtaaaagtg aaattaaacg tgatgccgag gagctaaaac gccttggcgc
    18781 ggaaattgtt gatctgggga aaaacgcgct ggataagatc ccgttagatg cggatctgcg
    18841 cgcggctatt gagctcgccc agcgtattaa gatggaaggt cgccgccgcc agttgcagct
    18901 catcggtaag atgctgcgcc agcgcgatgt agaaccgatc cgtcaggcgc tggataagct
    18961 gaaaaaccgt cacaaccagc aggtggtgct gttccataaa ctggaaaacc tgcgcgatcg
    19021 tctgatcgat caaggtgatg acgccatcgc tgaggtatta aatctgtggc ccgatgccga
    19081 tcgtcagcaa ctgcgtactc tgatccgcaa cgcgaagaaa gagaaagaag ggaataagcc
    19141 gccgaaatcc gcacgccaga ttttccagta tctgcgcgaa ctggcggaga atgaagagta
    19201 attttcgttg ttgatgtgag tatggtttgc cggatgcggc gtaaacgcct tatccggcaa
    19261 tgctatctca ggctgaggcc gaattactgc gcagcttccg ccttcttcgc cagaccatcc
    19321 agcagtttct gatggatccc accaaaaccg ccgttgctca tcaccagaat atggtcgcca
    19381 ggctgagagg ttttcaccac catatctgcc agcgtatcca catcgccact ccagtgtgca
    19441 ggctgaacgc aggcttctgc cacttctgcc acctgccacg gaatatgcgc cggttgcagc
    19501 aggaagactt catcggcacg acctaatgaa ggtgccaaat cgtctttgca gatccccatt
    19561 ttcatggtat tcgagcgcgg ttccagcaca gcaataatgc gcgctgtacc accaactttg
    19621 ccacgcagcg ccgccagcgt tgccagaatc gccgtcgggt gatgggcaaa atcgtcatat
    19681 accgtgacgc cattcgcttc accacgcaac tccagacggc gacgagcatt aataaacgaa
    19741 cccagcgcgt tagcggtatc tgccggcgct acgccaacat ggcgggccgc cgcaatcgcc
    19801 atcaggccat tatgcatatt atgttcgcct accagcgacc atttcacttc gcccactttt
    19861 tcgccatcca gcaagacttc ccattcggaa gcatcggtgg tcagcttttt cgcctgccag
    19921 tgcccctgct cacccaccag ctcctgctcg ctccagcagc ccatcgccat ggtctgtttc
    19981 aggttgatgt cattttccgg ccagataata cggccctgcc ccggaacgat acgcaccaga
    20041 tggtggaact gtttctggat cgctttcagg tcgtcaaaga tatcggcgtg atcgaactca
    20101 aggttgttga ggatcagcgt acgcgggcag taatggacaa atttagagcg tttgtcgaag
    20161 aaggcgcagt cgtactcatc cgcttcgata acaaagaagt cgctttcgcc cagacgcgcc
    20221 gaaacctcaa agttccccgg cacgccgccg atcacaaatc ccggtttgta accacactgt
    20281 tccagaatcc aggtcgccat tcccgcggtg gtggttttgc cgtgtgtacc ggcaacggcc
    20341 agcacccagc ggtcgcgcag cacaaaatcg tgcagccact gtggacctga catataaggg
    20401 atgttttttt ccagtaccgc ttccacacac ggatttccac gggtcatggc gttgccaata
    20461 atcaccagat ccggctgcgg atcgagctgg ctggcatcgt aaccctgaat cagttcaatg
    20521 ccttgcttct caagtaaggt gctcatcggc ggatacacat tggcgtccga acccgttact
    20581 tcatggccta actggcgcgc cagcatcgcc agaccgccca taaacgtgcc acaaattcct
    20641 aaaatatgaa tgcgcatacg tcactatcct tttttaatct gccgctcatt ttacgcatat
    20701 gttgggcaag tgagaaacgc atttcaggat aatccgtaat ttgctggcgc gattcacctg
    20761 agcgcaacat tgagattatt tgttaagatt gttgcggtcg ctttactcca taaacattgc
    20821 agggaaagtt ttatgaaaac gttaggtgaa tttattgtcg aaaagcagca cgagttttct
    20881 catgctaccg gtgagctcac tgctttgctg tcggcaataa aactgggcgc caagattatc
    20941 catcgcgata tcaacaaagc aggactggtt gatatcctgg gtgccagcgg tgctgagaac
    21001 gtgcagggcg aggttcagca aaaactcgac ttgttcgcta atgaaaaact gaaagccgca
    21061 ctgaaagcac gcgatatcgt tgcaggcatt gcctctgaag aagaagatga gattgttgtc
    21121 tttgaaggct gtgaacatgc aaaatacgtg gtgctgatgg acccactgga tggctcgtcc
    21181 aacatcgatg ttaacgtctc tgtcggtacc attttctcca tctaccgccg agttacgccc
    21241 gtcggcacgc cggtaacgga agaagatttc ctccagcctg gtaacaaaca ggttgcggca
    21301 ggttacgtgg tatacggctc ctctaccatg ctggtttaca ccaccggatg cggtgttcac
    21361 gcctttactt acgatccttc gctgggcgtt ttctgcctgt gccaggaacg gatgcgcttc
    21421 ccggagaaag gcaaaaccta ctccatcaac gaaggaaact acattaagtt tccgaacggg
    21481 gtgaagaagt acattaaatt ctgccaggaa gaagataaat ccaccaaccg cccgtatacc
    21541 tcacgttata tcggttcact ggttgcggat ttccaccgta acctgctgaa aggcggtatt
    21601 tatctctacc caagcaccgc cagccacccg gacggcaaac tgcgtttgct ttatgagtgc
    21661 aacccgatgg cattcctggc ggaacaagcg ggcggtaaag cgagcgatgg caaagagcgt
    21721 attctggata tcatcccgga aactctgcac cagcgccgtt cgttctttgt cggcaacgac
    21781 catatggttg aagatgtcga acgctttatc cgtgagttcc cggacgcgta atctcctgcg
    21841 cctctccaca cggagaggca tttttatcgc tgcgcaatgt tgactcttgt cactggcgaa
    21901 ctctgacgat tctcccacag caccgttaac ccacgctgta atgctataaa aataaacaac
    21961 aaaataccga tggcgatttt cgtccaccag gaactcagcg tgccgtcaaa gttgatgtaa
    22021 gtctggatca gtccctgaat tgccacgcca aaaagcgtcc caagcaccgt cccgacgcca
    22081 ccacttaaca acgtaccgcc aatcaccacc gacgcgatgg cgtccagttc cacacctacg
    22141 cccgccagtg catatccggc ctgggtataa atcgagaaga caatccccgc cagtgttgcc
    22201 agtccggtgg agagcatata aatgcgaata gtggtgctgc gagtagaaat ccccatcagg
    22261 ttcgccgacg tcgcgttgcc gccaatagcg tacacctgat tgccaaaacg ggtacgatgc
    22321 gcaaggaaaa taccgataac caccaccgcc agcatcaaca gccccatcgc actaaggcga
    22381 ccgccgccgg ggattttcca cgcaaggctt gagagcgtgt cataaatggg atggtttatc
    22441 ggaatcgact cttccgaaac gagatagctg acgccgcgca aaaagaacat ccccgcaagc
    22501 gtaatgataa atgccgggat cttcagggcg tcgatcagaa gccccataaa tgcgccgaag
    22561 gcacagccca tcaccagcac tagcggaaac gccagcagcg gcgagaggcc gaaatcgcca
    22621 accacttttg ccagaaacac gccagtaaag gcgatcaccg aaccgacgga gagatcgata
    22681 ccgccagaga ggatcacaaa ggtcatacca acggcaataa tccccagaaa agcgttatcg
    22741 gtcaggatat tgcagatcac tcttgtggaa gcaaaaccgg gaaactgcgt caggcagtag
    22801 aaatagccca gcacaaagac gccgatggtg atcatcagtg gcaaattacg ttttatcacg
    22861 gctacgtact cctttaatca gactgataaa gcgttgcgac tggacaatca gcgcgcaaag
    22921 caccaccacc gctttcacca cctggttcat ctccggtgga aagcccgaaa gcaaaattcc
    22981 ggtgttcatc ccctgaataa tcagcgcccc cactaccgag agcagcaggt taaagcgccc
    23041 gcccatcagc gatccgccgc caatcaccac cgcgagaatg gcgtccagct ccagccataa
    23101 tccggcgttg ttggcatcgg caccgcgaat atccgccgcc acgataatgc ccgcaatcgc
    23161 cgcacacagc ccgctcaaca cataagtgag catgacgatg atccgcgtgt ttaccccggc
    23221 attttttgcc gcccgaatgt tgataccaac ggcttcgata aacatcccca gtgccgtttt
    23281 gcgggtcaac agccagaaca ggagaagcgt cagtaccgca ataatgactg gcgttggcag
    23341 gaacaacagc gatccactgc caaaccatga gagatccggc gagttaaacg tgacgatctg
    23401 tccggaggtg atcagttgcg ccacgccgcg cccggcgacc atcaggatca gggtggcgac
    23461 aaatggctga attttgagga tcgctaccag tatgccgttc cacaatcccg ccaggatgcc
    23521 ggtgcccagg gcgcttaaca aaacaatcgg caggctgact tccgcgaccg tcatcacagc
    23581 cgttgtggct ccggcgatag ccatcaccgc ccctacggag agatcaatcc caccggtggc
    23641 gatcaccagc gtcataccaa tcgccagtaa cgcaacgggg gctgcacggt taagaatgtc
    23701 tatggggcta ccgaacaaac gcccatcctg aagcactacc tgccagaaat gcggggctac
    23761 caggctgtcg accagcagca ccagcaatag tgctaccagc tgcggcatcc ccgtcggcca
    23821 gcgaaagcgc ctttttggcg tagtggtgtc cggaagagat tgaggcatca cactgttctc
    23881 cttacgccgc aatggcgttc atgatagccg gaacggaaag ctctgccagc cggatctccg
    23941 ccacctgttt gcgatcgagc atgatgatca cccggtcggc atagcccacc agttcttcca
    24001 gttcggagga gatcaccagc agcgccagac catcggcgca tagcgtttca atcaggcgga
    24061 tgatctcggc gtgggcacca acatcaatgc cacgggttgg ctcatcgagg atcagaaatt
    24121 gcggtcgggt cagtagccaa cgtgaaagca acactttttg ctgattgccg ccggagagaa
    24181 attcaatcgg ttgttcagtt gaaggtgtgc gaatgccaag ctggcggata aagcgttcgg
    24241 caatctcttg ctgctctttg cgggaaatgg gtcgtagcca gccgcgctgg gcctggagag
    24301 cgaggatgat attttcccgc accgaggcgg cagcgatgat gccatcggtt ttcctgtctt
    24361 ccgggcagaa gccgatgccc agtaccgaag cctgatgtgg cgatcgcagg ttttgcggtt
    24421 tgcctttgat caacgccgtg ccgctgtcag caggtttgat accgaagatc acttcggcgg
    24481 tttcggtacg tccggatccc agcaagcctg ccaggccgac gatctcgccg gggcgcactt
    24541 cgagatcaaa cggtgctatc gttccttttt tgccgtaatt tttgaacgcg gcaacgggtt
    24601 tgtcgctcaa caatgttcgc ccggcacgct gtagcgcgtg ggtatccagc tcgcgcccca
    24661 gcatcatttt taccagttcg atctgcggta gctcgcgcgt ttcccgacag cctacgaaac
    24721 tgccgttgcg taaaacggtg atccgatcgc tgacctgata gatctgatcg agaaagtgag
    24781 tgacaaagat caggctgacg ccgcgatcgc gcaactgacg catcaggcca aataatagct
    24841 ccacttccta ggtgtcgagg ctggcggtgg gttcatcgag gatcagcact ttggcggaga
    24901 gatcgatagc ccggcaaatg gcgacgattt gctgcatcgc gactgaaaag cggttgagcg
    24961 gttcgcgcac gtcgagggag aaaccgtaag atgccatcag ttcggtggcg cgcttttcca
    25021 tctctttgcg gcgtagaagg ccgaagcgtt tgggttcgcg gcctataaat agattatcag
    25081 cgaccgacat attgggtagc aggttgactt cctgatagac ggtgccaatg ccgagttgtt
    25141 gcgcgtgggc ggtatttttc ggtgagataa cctggccttc cagccagatg gtgccgcgat
    25201 cggcgtggta tacaccagtt aatgctttga ttagcgttga ttttcccgcc ccgttttcac
    25261 cgagcagcgc catgatttcg ccacggcgca ggctgaaatc aacgttgtct aacgctttga
    25321 cgccggggaa aaatttactt aatccttcgg tgcggaggat ttcctggtgt tggtcggtcg
    25381 tcatggtttc ccccggtgta aagcgtgctc ccagtaggca tgataagacg cggtaagcgt
    25441 cgcatcaggc ttcagtgcac catcgccgga tgcggcgcga gcgccttatc cggcctacga
    25501 atcggcatca ggcatagcaa ccctcaatac cccatatttt tcttcttctc taactcttct
    25561 tttgcggtat caggcaggta aagggtggat ttggttaacg tcagcttttc aggcatggtg
    25621 ccgtcttttt tgtatttctc cagcgcgtca aaagcgggtc ccgccatatt cggcgtcagt
    25681 tcaacactgg cgttcgcttc gccatcaatc atcgctttgt agatatccgg tacgccatcg
    25741 atagaacctg tcagaatatc tttgcccggt ttcaggcccg cttctttaat tgcctgaatt
    25801 gcaccaatca ccatgtcgtc gttatgggcg taaaccatgc agatgttttt gccgttgttt
    25861 tccgctttga taaagctctc catgacttct ttgcctttac tgcgggtgaa gtcacctgac
    25921 tgcgagcgga taattttgat atttggcgca ttcttaatgg cttcggcaaa gcctttctta
    25981 cggtcaatgg cgacgctggc cccaacggtg ccctgcagct ccaccacgtt gcatggtttg
    26041 ccattcactt cttttaccag ccagtcaccg atcaacttac cttcgaggat gttgtcggca
    26101 gtaacggtgg tcatatagag agatttgtct ttcacatcga tggaacggtc gagcaagaag
    26161 accgggattt cggcatcttt cgcctctttt aataccggtt cccaacccgt cgcaaccact
    26221 ggagcgataa agatcgcatc caccccttgt gcaacgaagg aacgtaccgc tttaatctgg
    26281 ttttcctgct tttgctgacc atcggcaatt ttcaacgtaa ttccgcgttt ttcggcctca
    26341 cttttcgcca cattggtttc tgcggcgcgc cagccggatt ccgatccgac ctgcgaaaat
    26401 cctacggtta atggagcggc caacgccata gacgacatgg ctgccgagac tgcagagact
    26461 acaagtaagc gtttccacat agggttgtcc tcgtcggggt aattattatt gatgaaaaga
    26521 tgttcgcgaa aaaactatag acaattcgtt atgtaacgga ttgcgttaca tcacacttca
    26581 aaaaagtaaa taaaacgtta atcacaagtt tgtaatcgct ttcatctcac tatgaaaaat
    26641 gcggctacgg ttatggattt tcctgctctg tataccgtct taaaactggc gaaaaaggaa
    26701 aatgaagacg aaaacaagca aagacattcg gcgcgagttg gctataatac tcggcacttg
    26761 tttgccacat atttttaaag gaaacagaca tgagcttact caacgtccct gcgggtaaag
    26821 atctgccgga agacatctac gttgtcattg agatcccggc taacgcagat ccgatcaaat
    26881 acgaaatcga caaagagagc ggcgcactgt tcgttgaccg cttcatgtcc accgcgatgt
    26941 tctatccgtg caactacggt tacatcaacc acaccctgtc tctggacggt gacccagttg
    27001 acgtactggt cccgactccg tacccgctgc agcctggttc tgtgatccgt tgccgtccgg
    27061 ttggcgttct gaaaatgacc gacgaagccg gtgaagatgc gaaactggtt gcggttccgc
    27121 acagcaagct gagcaaagaa tacgatcaca ttaaagacgt aaacgatctg ccagaactgc
    27181 tgaaagcgca aatcgctcac ttcttcgagc actacaaaga cctcgaaaaa ggcaagtggg
    27241 tgaaagttga aggttgggaa aacgcagaag ccgctaaagc tgaaatcgtt gcttccttcg
    27301 agcgcgcaaa gaataaataa gttcttctag cgcaataacc ctgaacgccg ggcttcggtt
    27361 agtaagggtt tttttatgcc cgcgataaat aaactctcta ttccaccatc attattctca
    27421 gcggttgcaa ggcttgaacg gtaagaacaa gcaaacccga ccaccatttt gctgttcata
    27481 gccacttgct ggaagttagc cgacctcact catactcacc gttcaaagcg tgcccccgtt
    27541 ttacccctgc acatttgtga acgacagcag agttttttat gcgattgcag atatggaaag
    27601 cggctcggag agaaaagcaa aagatggaaa agttacaaaa aaaaatcgcg agatacatcg
    27661 cataaaacaa cgccacccga aggtggcgtg cttttattat tacttatccc tgtctaacca
    27721 gtcgccgctt tcaattagct ttaatccctc gacgggtcgt tgataaacgt acatccatgc
    27781 actcccgtac ggcgtctgaa tcaactggcg cgcgtattca ccgcccctgg tgcgcaaggc
    27841 atcaagttcg gccagcgtgg cgttgtcaat acgataaact tcaccgtgta ccgttccgtt
    27901 ccccggaact gcgcctggat agtggcccag gctatacaac tggtagttat cgatactgaa
    27961 atcgcccagt aactgggcat tggtcatcca gtgactgttg ccttgtttgt ggcgtaaact
    28021 gccgtagaca aatattcgca ttgctaaaac tcgaactgat agagcaaatc cagtgcctgg
    28081 tctacaccag acacggcttc cagatatagc ttaggcatca ggcgataacg taacgtgagt
    28141 gttgctatag agtcaaatat acccacgccg tatttcactt gcagacctgg caatacatag
    28201 ccgctgacca ctacctggga ggagtcgcct actccctggg tgtcgagcgc taaattgctt
    28261 acgccaaacg tctcgccgat tttacccaca atctggccac tttgcgcaac ctccagacca
    28321 atcagcatcg aggtcattgc cgcactgtcg ctctgatcgc tctccagccc ttgtccacgt
    28381 agcaaataag acaatgcagc ttgttgcgac atcgccgggt cagagaagat ctccgctttc
    28441 ggttcgtccg ccagaccagt gacgcgaact ccggcgatta cgtcgtcttc tgtagcatcc
    28501 gggttacgaa tagcttcaat attaagatac ggttgatctg gcggaccaga gaacagtagc
    28561 tcacccttac gcacaatcag atcctgacca taggcatgga agcgcccttc agggatgttg
    28621 atctgcccgt tcaggcccag cccttgtttg tcctgaacga cgttaagatc gcccgtcagc
    28681 cgcgctttca ggccaaaggc gtcaatgcgc acattgttgc caacgtggac aatcaggtta
    28741 ctgttaatcg gaatcgacgc cgttttcggc tcttccggtt gcaggttatc gttaagcatc
    28801 accacatcgc tggagacgcc tactgcgctt tccggcagat cgtgcaccac gatgcgcgcc
    28861 cacgggacat ccacgcgacc atcgagggta aacaggtttg gtgtagcctc gaatacaaca
    28921 tctggcgata catccattcg taccatcggc ggcacggtga tccgcacttt actgcctttc
    28981 gccgttactc gtgcccgcca gttttcaatt tggctccagt cggcatcacc gttcaggtag
    29041 atttcaccct gctgggtccg tactgtaccg gcaagcgtcg agcgcatacc gttaaagttg
    29101 accgcaagct ggctcggctg catatcaaac ggcataaagt tgccgtcgat atccacaccc
    29161 gtaacctgaa gctgaccaaa caactgcggg ctttgcacat caccacccag acgcaagttg
    29221 gcactcacca tccccgctgc tttttcccca cgagtaaaga tggggtttat catcgcaagg
    29281 ttgaagttac ggatattgac gttgccacca agattacggc ggccttgcgg atcggtcacc
    29341 agcacctgtc catcaaactg gccgttattg gtcaggcgga tggtccagcc caattcggca
    29401 cggttgttac gcaattccgc cgtcagattc agtgtctgaa acgccaccgg cagcgccgca
    29461 tcgttgacgg tttgcgttac ctgcacgtta cgccccgaaa gggtgatact gccctgcggc
    29521 agcccctctt tcgtggtgtc ccaggcaaca tccgctttac ccgtgaagat accgctggcc
    29581 tgagtggttt ctggcataaa cggtttcagc atggcgaagt cgaagcggtt gagattcacc
    29641 accgcacgcc cttcggcccc cgcatcgata gtttgcggca cgcacagttc cgcattcggg
    29701 ttaagccagc agtgtggccc gatgctgatt ttttgctcct tattgcggta atccagcgca
    29761 atatcgcggg tcagcgacca cgggccgacc agcgtctgga agcgggtatt gctaagagtt
    29821 cccttccaac gttcttcttt gcgatcaaaa cttcctgcca gattaagctg cccggagaca
    29881 ggctcgccct gaatccgcaa ctgtagctcg tgctgctttt cgctgccttt ggcattcagg
    29941 gtgacgaggt tgatatttac atccggctgc gaaatttgct caacgcgtac gtcgagtttc
    30001 ccggcgatct gatcggtgga tttaatgtcg ccttccacgc gaacctgcgt cacggaaagt
    30061 tcctgccagc gcaggccgcg cgcggtgata tctgccagta gttgtggcgc ttccaccgtg
    30121 ccgcgtactt tcaccagccc tttcgctgta ccgccaagcc ccggcagcgc gttatccagc
    30181 cccggcgcgt tgatggtggc atcaagattg agatctttta cccccagctc gcctttcact
    30241 tcggcactgt ttggccccag ttccagatga agcccaggga tcatccactg catataactg
    30301 ttgcctttca gcgtgccgtc aacgttcact ttgttctgtt tgacgttacc ggtcagcttc
    30361 agttctggca cctccatctg ccaggtgccg ccgtacaggc taccgcgggt tttaatcaag
    30421 ccattgagtt tcgacggcca ctccgggaac tctttggcgg tgttaatgcc attaagcgtt
    30481 agctcaccgc gccaactaat ggcctgctgc cagtcgagca acgccttgag ttcagttttc
    30541 ccttccagcg ccgcgacggt gagtttgtcg agattgacct gctgttcatt acctttggca
    30601 tcaagggtaa tggtggctgg cgggatctcc tgtcccttca ctgccgtacg catagagagc
    30661 gtgtaatcgg tcattttgcc ggtaagtttc agtttcagat catccgcctg atactgcttc
    30721 tcaccagtga acggccagta aagctgtttg ctgttcactt ccacgttgag cggcaatccg
    30781 gcttccgcca gtcgcgtctg ggcgcgtaaa tccatatcca ccggaccgga aagattaacg
    30841 ccaatctcca gctgttcgcg cagcgcgccg cccactttta gcttcacttt ttcacctttc
    30901 aacggctcca cgttcagtgt gctgttgagg gtgatatcca ccggccagtt gtctgacagc
    30961 tgcgccgtgc cgctggcgtt gacgatcccc tggttggaat cgatatccag ggcgtccagt
    31021 ttagtattgc cgtcaatgct gctcactttc agcagcatgg tgcgcacggt gatgtccgtg
    31081 tcgcccgtca cgcgtaactg ctcgccttta aactcttcaa tgttcaggtt aagcggcaaa
    31141 tgcaagtcgg tcatttccgg caataccggg cgaaaaaaga gatctttcag cgtttcgccg
    31201 agcggctttt catccggctg cggattttca attttcggtt cgaccacttc ttcctgcgcc
    31261 acttccgcca ctttcggcag agcaatcagc aggcctttca gcgacgtcgg tttcagggtc
    31321 agggttttct cctgccagtt caggccggag gtgaagtcca tcaccgacac cgtggtgtca
    31381 tcaatcttga tgttgacgtt gtccagcgcc acccgtgtca gggtgatggg atacggcgtg
    31441 gagagatcca gcggaccgct atcttcctct tcttcaacct gttcagaagg aggcattttt
    31501 ttactgtcga tgttgacctg aatgtctttc agcgccaggt cattaataca aacgctactg
    31561 ttccacaggc actcaagacc gaccgccaga tgcagattac ccgcttttac cgccacgcct
    31621 ggctgctcat aacgaacgtc agacaaggtg agatcgcgcc agccgccggt gaccttgcca
    31681 atatccagtc ctggcaccca gcgatccgcc gctttaaata caagatgcag gccgctggtg
    31741 gtgcccacca gaaacgccac cgatcccagc aacagtaaga taacgataac cacgccgagg
    31801 ctgatttttt tccataaact cataattctg gccccagacc gatgtaaaac tgtaacccgt
    31861 gttcgtcttt atccgcgacc ggtacggcaa aatcgagttt gattggcccg accggcgatt
    31921 cccagcgcac gccgaccccg gtaccggttt taaagtcgct gcggcgaata tcgcttaccg
    31981 cttcgccact atcgacaaac accgcgcccc accattttcc ggtcacgttg tactggtatt
    32041 ccagcgatcc ggttatcaac ttcgaggccc ctttcaggtc accgttggcg tatttcgggg
    32101 cgatagattt gtatttgtag ccgcgaatac tgcgatcgcc cccggcgaaa aaacgcagat
    32161 ccggcggtac tttgtcgaaa tcaccggttt caatccagcc cagcgtgccg cgtgtaacga
    32221 aacgatggcg atcgtacagt gtgcggatcc agacgttctg cgcctggaaa acggagaaat
    32281 cgacatctga gccccaggcc gtgttggagt agtcgataga gtagcgttgc gagtcgcccc
    32341 aggttggcat caggccacca cgagaacgcg tgcggctaat catcacccca ggataaaaca
    32401 gcatcgtggt gttggtaatt tcaccctgag taaagtggtc gagactccag cgcaggttaa
    32461 tggcacgctg ccagccgctg gagagatccc agtagcgaga agccaccagc gtagtggagt
    32521 cagattcggt atcgttcagg tcagtgcgct taaaaccgcc ctgcaccaaa taatattgtt
    32581 ccagtggatt cttcagcagc ggcattttat agctgaagtc gagggtctgt tccggcgcgg
    32641 aaatactggt actggtggtc aaactgtgac cgtatgagtt catccacggc tttttccacg
    32701 tcgctttcac gcgcggtccc acgtccgtag agtaaccgac cccggtttcg atggtgtttt
    32761 ctgttcgcgg cgaaaccacg cccgtcaatg gtaatacttt cgtttcgcgc gctttatcaa
    32821 attgtggagc caccaccacc gagttaaacc agccggtagc agaaagtcgg cggttcagtt
    32881 ccgccagatc tttcgattca tactcatcgc cctctttaaa cggcaccaga ttttgcaggt
    32941 attcatcgcg gatttgtgat ccttcaaagg tcacatgccc aaagcggtaa cgttcgccac
    33001 tgttataatc aatatcccag aaggctttat gcaggccgag cgcaatgccc agctgcgctt
    33061 tggtaaattc gctatcgaaa taacctttac gcaacgcaat actggttaag gactttttga
    33121 aattttcata atcgccctgg ttcagtaccg taccaatagc cgggcgagta tcgagcaatt
    33181 tcaaatagtc tttatcggtc cgcgcgccgc cgcgcaatac cacatcggtg ccgccaatta
    33241 acaccggcac acctggcgtg actttggcga tcaatacctg ccgccctttc tttggcggtg
    33301 gacggagatc aaattcaatg gtcggctggt aataacccag cgctttcaga ccttcacgga
    33361 tggcatcatc gacgcgtgcg cgaaagcggc ggtctggcgt cacttcatca ctttcaatcg
    33421 tagaaagctg cgcacgaacg ttcttttcca gctgtcccga taacccctcg acctgtagac
    33481 ggacgttcgc ggcgacggca gatccgctta agcagagtaa gcttacacag cataactgtc
    33541 ggatatagcg cacattttct cctgaatatc cttttttcct gcccctggaa cgccgttaaa
    33601 cggcttaaca aaaatccagt aatatggatt aaaaagcaga ctaaacccca aatatttctt
    33661 atgtttaact ttagacctat tcacggtggt tattgtgtgc aaatacgcct cttgttacaa
    33721 ccttaacccc aatgaccgat tttcgggaga gcgacaccat gagtttattt gataaaaagc
    33781 atctggtttc ccccgccgat gccctgcctg gacgtaacac cccgatgccc gtagccacgc
    33841 tgcatgcggt caacggtcac ttaatgacca atgtacctga cggaatggag attgccattt
    33901 ttgcgatggg ttgtttctgg ggtgtggagc gtctgttctg gcagttaccc ggcgtttaca
    33961 gcaccgccgc aggctatacc ggcggctata cgccaaatcc gacttatcgg gaagtgtgct
    34021 ccggtgatac gggtcatgcc gaagcggtac gcattgttta cgatccttcc gtcatcagct
    34081 atgagcagtt gctacaggta ttttgggaga atcacgatcc cgcccaggga atgcgtcagg
    34141 gcaatgacca cggcacgcag tatcgttcag cgatttatcc gctgacccca gaacaggatg
    34201 ccgcagctcg cgccagtctg gaacgttttc aggcggcgat gcttgccgcc gatgacgatc
    34261 gtcacatcac cacggaaatc gctaacgcca caccgtttta ttatgccgaa gatgaccacc
    34321 agcaatatct gcataaaaac ccatatggtt actgtggaat tggcggaatt ggcgtctgtc
    34381 tgccgccgga agcatagcgt tacgggtaca aatgtagttt gttgataaag tgctctttgt
    34441 ctatgcctga tgcggcttaa acgacttacc aggcctacaa aatcgtccaa attcaatata
    34501 ttgcaaggac tgcgtaggcc tgatcggcat agcgcatcag gcagtattgc atttatcagc
    34561 agtctgaatt ttaaccctct ggcgacttta cagtacctta cgctatacta gccactgaaa
    34621 atgccggttc actttcttcg aatcggcttt caatgtgtat ttcacacaaa ttaatcaact
    34681 ttcccttccg aggatctggc ctgaaaggtc ggataagata tgttaaacag tattttagtc
    34741 atactctgct tgatcgctgt aagtgcgttc ttctcgatgt ccgagatctc gcttgccgcc
    34801 tcacgcaaaa tcaaacttaa actgctggct gatgaaggca atataaatgc ccaacgcgtt
    34861 ctgaatatgc aggaaaatcc cggcatgttc tttaccgtgg tccaaatcgg tctgaacgca
    34921 gtggcgattc tcggcggtat cgtcggtgat gcggcatttt ctccagcttt tcacagcctg
    34981 ttctcccgct atatgtcggc agagctctct gagcaactga gctttattct ctctttctcg
    35041 ttagtgactg gcatgtttat cctgtttgcg gatttaaccc cgaaacgcat cggtatgatt
    35101 gcgccagaag cggtggcttt gcgtatcatc aacccgatgc gcttctgcct gtacgtttgc
    35161 accccgctgg tgtggttctt caacggcctg gcgaacatga tcttccgtat tttcaaactg
    35221 ccaatggtac gtaaagatga catcacttct gatgacatct acgcggtagt ggaagccggt
    35281 gcgctggcgg gcgtgttacg taaacaggaa cacgagctga ttgaaaacgt ctttgagctg
    35341 gaatcccgta ccgttccgtc ttcaatgaca ccgcgtgaaa acgtgatttg gtttgatctc
    35401 cacgaagatg agcaaagcct gaagaataag gtggcggaac atccgcactc taaattcctc
    35461 gtctgtaatg aagatattga ccacatcatc ggttatgtcg attctaaaga cctgctgaac
    35521 cgcgtgctgg ctaaccaaag cctggcactg aacagcggcg tacaaattcg caacacgctg
    35581 attgtgccgg atacgttaac cctttcagag gcgttggaaa gttttaaaac cgcaggtgaa
    35641 gacttcgcgg tgatcatgaa cgagtacgcg ctggtggtgg ggatcatcac cctcaacgac
    35701 gtgatgacca cgctgatggg cgatctggtc ggtcaggggc tggaagagca gattgtcgcc
    35761 cgtgatgaga actcatggct gattgacggc ggcaccccaa ttgacgacgt catgcgcgtg
    35821 ctggatattg acgagttccc gcagtcgggc aactacgaaa ccatcggcgg ctttatgatg
    35881 tttatgctgc gtaagatccc gaaacgcacc gattcggtga aattcgccgg ctacaaattt
    35941 gaagtggtgg atatcgataa ctaccgcatc gaccagctgc tggtgacccg gatcgacagc
    36001 aaggccaccg ccctttcgcc aaaactgcct gacgctaaag ataaagaaga aagcgtcgcg
    36061 taacccccag aaacatcaac ggctcctgaa tcaggagccg tcttattact gcatagcact
    36121 ttggttaagc catctctgtt tgcagacgca taacctgacg gttaacttcg gacatcactg
    36181 acaaatgcag cttatccttc acttttggga taagaatctt acccttatca aattcaaaag
    36241 cgccaacgtc cttgatgtat aaccgtccac ggaacaggat ctttacgtac ttcgccacct
    36301 gaagtggatt gtaccgttgg aaaattttca ttgttatctc ctgctgagta ttacgcctgt
    36361 gcggttccac aatcggccca actattatga gacgcaaatt ttaatgctta gtgactatag
    36421 actatccggg caatgtttcc accgcgtata acttttttta cctaaaggtt acaattattc
    36481 agaattatct ttttaccgaa gcgcggtctt cagtataagc attcattttt catatgattt
    36541 gtgcgcttga ccgcaaactg gcatcacact tgcgggaaat tcgataaata gcacatatga
    36601 ttaaaactca gatccaagtg gtcggatcac ctgcatatca taagaaggaa acaccatgac
    36661 cctacgcaag attctggcac tcacctgcct gctgttgccg atgatggctt ccgcacatca
    36721 gttcgaaacc ggtcagcgag tgccgccgat tggcattacc gatcggggcg agttggtgct
    36781 tgataaagat cagtttagct acaaaacctg gaacagcgcg cagttagtgg gaaaagtgcg
    36841 agtactgcaa catattgctg gtcgcacctc tgcaaaagag aaaaacgcga cgctgattga
    36901 agcgattaaa tcagcaaagt taccgcacga tcgttaccag accaccacca ttgttaacac
    36961 cgacgacgca attccgggtt ccggcatgtt tgtgcgcagc agtctggaga gtaataaaaa
    37021 gctttatccc tggtcgcagt ttattgtcga tagcaatggc gtcgcgcgcg gtgcctggca
    37081 gctggatgaa gaaagttccg ctgtagtggt gctggataag gatggtcgcg tgcaatgggc
    37141 caaagacggg gcgctcacac aggaagaggt gcagcaagtg atggacctgc tgcaaaagtt
    37201 gctcaaataa tggcaataac ataaattctg caaaaataat tttcccctat cgttatctat
    37261 gggatagggg aattaggtct ctgagctctt acaccgaact catactggta acgctagttg
    37321 ctctttttta aacgcctcaa tggttgattt atatttctcg caaatctcct cttcggtaat
    37381 atcactatct acggccagat tcgcacttac tatcgcatcc acttgttcgg ctaattcgac
    37441 ttgttggcaa cctgcattaa ccagaaaatt ataaataaga gagtaatctt catcctccaa
    37501 aacaacagag gaaccattac gaaaacgttc atcccctcca tagattttaa gcgcaaagcc
    37561 caaagattct atgaatatgg caaatttatc atcaattatt ttttcgtaaa tataccatcc
    37621 atcgcattct ttaattccac ctcctggtat ttggagttca aatttacccc gttttaattt
    37681 gcttactata tcctccaata aggcataacc taaagacacg tcaccaatcg cctggtgaat
    37741 ataaccttta atccttttcg gtatgtcaga ctgttttttc tgcagaatat cccaggcata
    37801 caatcttctg ttttgttcta acggaaattc acggtttttg aaatccatga atgagttata
    37861 ctcatttatt gttatgggga tttcttcgta ttcaaatcca ctgcctcgca atgtcattgt
    37921 atattcggca ccgttatctt taaggctgaa actgataaga aacttatcca tggctttaac
    37981 agtttcattt tttgatgaaa gcacttgata actatcttta tcaatctgag ttgcgcaacg
    38041 aaaaaactcc cacaaagtta acaggctatc ataattcgaa acccaatctt ttttcttaaa
    38101 tgcatgctga atattattaa aaaaattgtt tgaatgtgtt tttgtatgcg atgattttac
    38161 gaataactcc accgcaccaa taaaattatt gtgtctgatc ctgggtaaca tatggctacc
    38221 ttattttatt tggttatttt atgtcgccat taaatatctc attatagaag taaactatca
    38281 aaaaacgctc attattaaaa tgtaacttat tagcaattga atttttgcac tttaaataca
    38341 atctcttttt atttataact ttttaggttg ggagaaatca ggatggcaga atcaggaaat
    38401 acactgtttc tgccatctga atttagtaaa tagacactct gaacctagga ttcaggaacg
    38461 actcgcgcgg agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag
    38521 ctgcggccac agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt
    38581 acagctgcgc ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg
    38641 tctgatgttc gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc
    38701 tgataaccac cagcggcggg cgcgcatctc ggacctgaat ctgcttgcgc acaccgcact
    38761 cttctttcca ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca
    38821 ccacacctaa aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat
    38881 tgcgtttaat aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt
    38941 gctgacggac ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgtca
    39001 gcgtacgtaa accgtccatg ataacggtgt gtgcggcaat atccgctgcc gttaccggag
    39061 aattgtccgc tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg
    39121 catcgcctgc attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct
    39181 gtgagcggtg ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt
    39241 gatagtgtca tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag
    39301 acgtttattc tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa
    39361 gtttagcgca acgctcctgg ctacgctgat tgccgccagt gtgaatgcag cgacggtcga
    39421 tctgcgtatc atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa
    39481 agacaccgcc acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg
    39541 caatgaagtg aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct
    39601 ggccgattac atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc
    39661 gctgaatacg ctggattata cggtcggtac actcggcaat catgaattta actacggtct
    39721 ggattacctg aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat
    39781 tgacgccaga accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt
    39841 cgataaagac ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca
    39901 aatcatgggc tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga
    39961 aaccgtgcgc aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtcg ttgtcgttct
    40021 ggcgcattcc gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta
    40081 cctcagtgaa attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc
    40141 gggtaaagat tttgctgata tcgaagaggc tgatatcacc aaaggcacgc tgaatggtgt
    40201 tccggcggta atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag
    40261 taatgacagc ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga
    40321 catcgccaat aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc
    40381 cgatcacgat gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat
    40441 gtatagctat ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca
    40501 aaaagcgtat gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct
    40561 ttcagctgcc gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga
    40621 ggtggaaaaa ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac
    40681 gctgattgta gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg
    40741 acagtttaac cagattgatc ctaacagcac gaaaccacag tcactcatca actgggatgg
    40801 tttccgcact tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca
    40861 acccgcccgc tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa
    40921 cctgaccttt aatggcaagc cgattgatcc gaacgctatg ttcctcgttg ccaccaataa
    40981 ctatcgcgct tacggcggca aatttgccgg tacgggcgac agccatatcg cttttgcttc
    41041 accggatgag aaccgctcgg tgctggcagc gtggattgct gatgagtcga aacgtgcggg
    41101 agaaattcac ccggcggcag ataacaactg gcgtttagca ccgatagctg gcgataagaa
    41161 actggatatc cgtttcgaaa cttccccgtc agataaagcc gcagctttta ttaaagagaa
    41221 agggcaatat ccgatgaata aagtcgcgac cgatgatatc gggtttgcga tttatcaggt
    41281 ggatttgagt aagtaaaaca cttctttttc ggtctataaa ttatcaaccg catccggcat
    41341 ttattggcgg atgcgatgct ggcgcatctt atccgcccta caagccatgc accgtagacc
    41401 agataagctc agcgcatccg gcagttatgc cgcacgttca tcccgcaccg ccaacacctc
    41461 gggcaaattc aactcaatcc agtccgccag tgcagcaacc ttttcgctca cctgctcgcc
    41521 cagcggcgtg aggctatatt ccacatgcgg tggcaccacc ggatacgcga tacggttaag
    41581 aaaaccatcc tgttctaacg cctgtaacga ctgcgcaagc atcttttcgc tcaccccgcc
    41641 gattttgcgc cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca ccagaatcaa
    41701 caccccccaa cggctggtga cgtgtttcaa cacctcgcgc gacgggcact gttccgcaaa
    41761 gagattaccc tctttcagtt gttgcgacag gctaacctga ctcatttcat acttaccttt
    41821 ttgtacgtac ttactaaaag taagtttagg tgttagcgta tttaaacaca agacaaaacg
    41881 atggagactt cccatgatcg ctattactgg tgccactggc caacttggtc actatattat
    41941 tgaatccttg atgaaaacgg ttcctgccag ccaaatagtg gctatcgttc gtaatccggc
    42001 aaaagcccag gctctggcag cacaaggcat taccgtgcgt caggctgact acggcgatga
    42061 agccgcactg acatctgcac ttcagggagt ggaaaaacta ctgctgatct cttccagcga
    42121 agtgggtcaa cgtgccccgc agcatcgtaa tgttattaat gccgcaaagg cggctggcgt
    42181 gagatttatc gcttatacca gcctgctaca tgcagatacc tccccgctcg gcctcgccga
    42241 tgagcacatt gagacggaga aaatgttggc tgattctggc atcgtttaca ccctgctgcg
    42301 caacggctgg tacagcgaaa actacctcgc cagcgccccg gcagcactgg aacacggcgt
    42361 atttatcggt gcggcgggcg atggcaaaat cgcctcagca acgcgggcag attatgcggc
    42421 agctgcggca cgcgtgatta gcgaagccgg tcacgaaggc aaggtttacg aactggcggg
    42481 cgatagtgcc tggacgttga cacagttagc ggcagagctg accaaacaga gcggcaaaca
    42541 ggttacctat caaaatctga gcgaagccga tttcgccgcg gcactgaaaa gcgtcggact
    42601 gcccgacgga ctggcggata tgctggcgga ttctgacgtt ggcgcatcga aaggtggtct
    42661 gtttgataac agcaaaacgc ttagcaaatt gattggccgc ccaacgacaa cgttagccga
    42721 aagcgtaagc catcttttta atgttaataa ctagttaatt aaagtggcat cctcccgcat
    42781 cctctctaat aatgacggga tgccgggagc aatcatgtct gcttcctgaa ctttcttctg
    42841 acagaccaat ggatgccagt aatgattagc ggcgtgctgt acgccctgtt agcagggttg
    42901 atgtgggggc ttatttttgt cgggccgttg atcgtaccgg aatacccggc gatgttgcag
    42961 tcgatggggc gttatctggc gttagggtta attgcgctgc ccattgcctg gctgggacgc
    43021 gtgcgtctgc gtcagttggc gcgtcgcgac tggcttaccg ccttgatgct cactatgatg
    43081 ggcaacctca tctattactt ctgccttgcc agtgccattc aacgtactgg tgcgcctgtt
    43141 tccacgatga ttatcggcac cctgccggtg gtgattcccg tttttgccaa tctgctttat
    43201 agccagcgcg acggcaaact cgcgtgggga aaactcgccc cggcactggt ttgtattggc
    43261 atcggcctgg cgtgtgtgaa tattgctgag ttaaaccacg gactccccga ttttgactgg
    43321 gcacgttata cctcaggcat cgtgctagcg ttagtttccg tggtctgctg ggcatggtat
    43381 gccctgcgca acgcccgctg gctgcgggag aatcccgaca aacatccgat gatgtgggcg
    43441 acggcgcagg cgctggtcac actgccggtt tctctcatcg gctatctcgt cgcctgttac
    43501 tggctgaata cgcaaacgcc ggacttctcc ctaccttttg gcccccgtcc gctggtgttt
    43561 attagtctga tggttgcgat agccgtgctt tgctcatggg ttggcgcact ctgctggaac
    43621 gtcgccagcc agcgattacc gacagtgatt ctcgggccgc tgatcgtttt cgaaacactg
    43681 gcaggtttgc tgtacacctt tttgatacgc cagcaaatgc cgccgctgat gacgctgagc
    43741 ggtatcgcgc tgttagtggt tggcgtggtc attgcagtca gagcaaaacc ggaaaagcct
    43801 ttaactgaat ctgtctcaga aagttgacat actggctgtg agttaaataa gcctctgcta
    43861 cgtaagggtt atagcttttg ccttaaagat gtatttaaaa tgcatcttat cttattaaga
    43921 atgaggtatc agctatggct tatcgcgacc aacctttagg tgaactggcg ctctctattc
    43981 ctcgcgcttc agctctgttt cgtaaatatg atatggatta ctgctgtggc ggtaagcaga
    44041 cgctggcgcg cgcggcggca cgtaaagaac tggatgttga ggtcattgaa gctgaactgg
    44101 caaagcttgc tgaacaaccg attgagaaag actggcgtag cgccccgctg gcagaaatca
    44161 ttgaccatat catcgtgcgc taccacgatc gtcaccgcga gcaactgccg gagctgatcc
    44221 tgcaagcgac taaagtcgag cgcgttcacg ccgacaaacc gagcgtgcca aaagggctga
    44281 caaaatacct gaccatgctg catgaagagc tttccagcca catgatgaaa gaagagcaga
    44341 tcctcttccc gatgatcaaa caaggcatgg gcagccaggc aatggggcca atcagcgtaa
    44401 tggaaagcga gcacgatgaa gcgggcgaac tgctggaagt gatcaaacac actaccaata
    44461 acgtcacacc gccgccagaa gcctgcacca cctggaaagc gatgtataac ggcattaatg
    44521 aactgattga tgacctgatg gatcacatca gtctggaaaa caatgtactg ttcccacgcg
    44581 cgctggcggg tgagtgatct aaaagctgga tggcattgcg ccatccagca tgataatgcg
    44641 gttatttccg cagttcagca gcccgcttct taccaataaa caaccagccc agccccagtg
    44701 cgataaacca cagcggggta accagcagcg cctggcgagt gtcatcttcc agtgtcagca
    44761 acacgaccac gaacacaaag aacgccatac atacccagca catcagcttg ccgagcggca
    44821 tcttgtagat cgacttctca tgcagatgag gacgctgttt gcggtacacc aggtacgagc
    44881 aaaggataat cgtccagacg aacataaaca gaatcgcgga aacggttgta atcatcgtga
    44941 acgcaccaat cacgctagga ttcacataca gcatcaccac gccaccgagc aggcagatgc
    45001 aggagaaggt cagccctttc gctggtactg cgcgcttaga aagtttagcg aacgctttcg
    45061 gtgccacacc ttcctgcgcc agaccaaaca gcatacggct ggtagagaag acgccgctgt
    45121 tagcggaaga cgccgcagag gtcagcacca caaagttgat cacgctggca gccgcaggca
    45181 aacctaccaa cacaaacagc tcaacgaacg ggcttttctc cgggactacc gaactccacg
    45241 gtgtcacgga cataatcaca atcagcgcga agacgtagaa cataatgata cggatcggaa
    45301 tggagttaat cgcgcgtggc agtgatttct ccggatcttt ggtttccgca gctgttgtac
    45361 ctaccagctc aatccccacg aaagcgaaaa ccgctatctg gaatccggca aagaagccac
    45421 ttaaaccttt cgggaaccag ccgccgtcat tccacaaatg cgcgaatgac gcttccacac
    45481 cagtcggtga ctgaaagtgc atcgccacca tgaccaggcc gacgacaatc agcgacacga
    45541 tggcgacgat tttgatcatc gcaaaccaga actccatctc accgaacatt ttcacggtgg
    45601 caaggttgag cgtcagcagc agcactatca ccgccagcga ggcgacccag tcggagagat
    45661 cggggaacca gaactgagca taagccgtga tcgccaccac gtctgccata ccggttacaa
    45721 cccagcagaa ccagtaagtc cagccagtga aatatcctgc ccacggcccg agtaaatcgg
    45781 aagcgaagtc actaaaagat ttgtattcca gattcgaaag cagcaattcc cccattgccc
    45841 gcatcacgaa aaagagcata aaaccaatga tcatataaac gaaaatgatc gacggcccgg
    45901 caaggctaat cgttttgcca gaccccataa acaaccccgt accaatggca ccgccaatgg
    45961 caataagctg aatatgtcgg tttgtgagat tgcgccgtag cgactgttca gccggagcct
    46021 gatcatcggc aacgactttt acctgatcta ccatgttttt ttcttcctgt acctgtctgt
    46081 gttgttcagg ctctacggcc tttagtcagt ctatgatacg acgaatctgt atcgggactc
    46141 atcgatatta ggtaacaata cgcgggatga atactaagat ttagaactaa tgttaatttt
    46201 atgtttaaag tgagtgtcat atcacggtga taacgcgaaa cagctcacaa aaatacacgc
    46261 ttacggggtc tttgcaacac aaaatagcgc cgtttggcaa actgaagggt ttattgctga
    46321 atgcctgctc ccctctggtt cgaggggaac agaatgcgct gcttagagga tttccagcag
    46381 ttcgacttca aacaccaggg tgctgaacgg agggatggat gcacctgcgc cgcgctcgcc
    46441 atatgccagt tcctgcggga tagtcagttc ccatttagaa cctaccggca tcagagtcag
    46501 tgcttcaatc cagccaggga tcacgccgtt aaccgggaat tcagcgggtt caccacgagc
    46561 aacggagctg tcaaacacgg tgccgtcgat cagtttaccg gtgtaatgaa cacgaacgcg
    46621 gtcggtacgt gccggaattg cgccttcacc ctggttgatc acgcggaatt gcaggccaga
    46681 ttcggtgcta ttcacacctt cttttttggc gttttcttcc aggtatttca caccttcagc
    46741 agccatcgcc tggaaacgct gacgacgaac ggcatcggcg cgttcgtgga tttcacgcag
    46801 cgcgcgatgc accacatcaa ccggaacagc cggatgtttg ccttccagcg catcggcaat
    46861 acctgcaacc agtgcttctg gcagcagccc ttccaggcca gattcactca gttgttgccc
    46921 tacctgcaaa ccaatgccgt agcttgcttg cgcttcgatg gtgtcaaaag ttggggtggt
    46981 catcgtattt cctttcttag ggtataaaag tagcgggcag catatcagcc acacttcttt
    47041 gggtaaaact ttgtcgcagg aatgatgaca aatcccgggg aaaaagaaac aatagcaata
    47101 tcccgtatga atacaataaa ggcgggttta ccacggacat catagcgtta gcttattcat
    47161 aggctatgat tgaggaacaa gacgcggagc aggaggaaac catgcccggg cgctttgaac
    47221 taaaaccaac cctggagaaa gtctggcacg cgccggacaa ttttcgcttc atggacccgc
    47281 tgccgccgat gcatcgtcgg gggatcatta ttgccgccat tgtattggtg gtcggatttc
    47341 tgcttccatc tgatgatacg cccaacgcgc cggtggtgac gcgtgaagcg cagttagata
    47401 ttcagtcgca atcacaaccg ccgacagaag aacagctgcg ggcgcaatta gtcacaccac
    47461 aaaatgatcc tgatcaggtg gctccggtcg ctccagaacc tatacaggaa ggccagccag
    47521 aagagcagcc gcaaactacc caaacgcaac cattccagcc agatagcgga attgataatc
    47581 aatggcgctc ttaccgtgtc gaacccggta aaacgatggc gcagctgttc cacgaccacg
    47641 gactgcccgc taccgatgtc tatgcaatgg cgcaggtata aggcgcgggt aagccgctga
    47701 gtaatttgca aaacgggcag atggtgaaaa tccgccagaa cgccagcggc gtggtgacgg
    47761 gcttaaccat tgatacgggg aataatcaac aggtcctgtt cacccgccag ccggatggca
    47821 gttttattcg tgcgcggtag atgaagggtc aaccaaaagc cagctaagga ttgtctcttt
    47881 gatgacctta cgtctggctt ccagtgcctg gggggaaacc atactgatat gataaaccag
    47941 gccaagcgta tgctggttga tcaaataata tccgccaagc gcagcaatat taatattcac
    48001 ctgcaatggg tcgatatctg gcttaaaaat attgtgtttc ttcccttcat ccaataagga
    48061 ttccattaac tgcaagtgcg catggttaat ttctaagaga cgctgtgatt ttgcataatg
    48121 cacaccttta ctttgatttt cactatgcac aatttttaaa aaccaggggt tagcaatata
    48181 ataatcccat gtaaaatcaa taattttctc aatagctttt tgtggcggaa gatctgagaa
    48241 atcaagggcc ttttcttttt gtcgaatatc gttccaggta tattccagta cttcaataaa
    48301 taaattttct ttattaccaa agtgatgata aaccgtctgt ttactacacc ccgcagcctt
    48361 aacaatatta tcgaccctgg ccccttcata accatattcg gcaaattcat taaccgcgca
    48421 taacagcagc ttttccttca ggctctgtac gttgctggta tcaagataca taacgcttcc
    48481 ccacgacctc ttcgtggtga ctatcataac ctgatttgat ttcagcttca gaaaaaaggg
    48541 aaaaaacttg tactttcatc aggttatcaa atgtcctgtg atcgtcatcg catatcaacc
    48601 aactagatgg ttgatctttt tatcctccag tcgcaagcta atcatcacca aggaaacaaa
    48661 attcaaaaaa gagagctatt gtatgaaatc cactcgtcct gttgccgtta ttaccggagc
    48721 cgctcgcggt attggaaagg gctgtgcgca ggagcttgct cgtggcgggt ttaacctgct
    48781 gattaacgat cgtcccgatg ccgacagcgt agaaaaactg catatcacac aacaagaatg
    48841 tttggcggaa ggcgtggaag tgatttgctt ccctgctgat gtcggtgatc tatctctgca
    48901 tgaggagatg ctcgacgcgg cgcaaaacca gtgggggcgg ctggagtgtc tgctcaataa
    48961 cgcggggatt tcagtgaaaa aacgcggtga cctcctcgac ctcgaaccag acagcttcga
    49021 tcaaaacatt gccattaaca cccgcgcgcc gttcttcctt gctcaggcat tcagtaagcg
    49081 cttactggca cagccgaaac cggaagcgga gctgccgcac cgctcgatta ttttcgtcag
    49141 ttccatcaac gccatcatgc tggcgatgaa tcgcggcgaa tacaccattg ccaaaaccgc
    49201 cgtttctgcc gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa ttggcgttta
    49261 tgaagtgcgc cctggtctca tcaaaactga tatgactatt ccggcaacgg cttattacga
    49321 cgaactgatt gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg ctgatattgc
    49381 ttccaccgtc cgcgcgatgg cggaaggtaa actgatttac acctgtggtc aggcggtcgc
    49441 catcgacggc ggcttaagta tgccgcgctt ctgaggctga atcatgagag atctgttaca
    49501 acgcccggat ttattcagta tcaataccgc gacactgggc tataaaacgc cgctacccgc
    49561 cattattgat gcctgcgccg cacgcggtat tggcgcaatt gccccctggc gcagggagtt
    49621 gcagggtgaa gatctccagc aaatcacccg ccagcttgcc gccagtaaca tgagcgtttc
    49681 cggtttgtgc cgtagcactt actacaccgc gccgacactg gcggaacgca aactggcgat
    49741 tgatgataac cgccgggcgc tggacgacgc agcggtgctt aacgccgcct gttatatgca
    49801 ggtggttggc ggtctgccaa cgggcactaa agatctatat gaagcgcgag aacaagtgaa
    49861 acaagggatt cgccagtcgc tgccacattc gaaagatgtc ggcgtgccaa ttgcgctgga
    49921 gccactgcac ccgatgacag ctgccgatcg ctcttgcctg tgcacgttac gtcaggcgct
    49981 ggactggtgt gatgaactcg atcccgatgg tgagtttggc ctcggcgtgg cggtggatgt
    50041 ttatcacgtc tggtgggatc cggatctcgc cagtcagatc ctgcgtgccg gaaaacgcat
    50101 cctcgcgttt catgtttccg actggttagt gccgaccact gatctggtca atgaccgtgg
    50161 tatgccggga gatggcgtca tcaatattcc gtcaattcgt cggctggttg aaaacgccgg
    50221 atttaatggt gctattgaac tggaaatatt ctcgccttac tggtggcaga aagatattaa
    50281 cagcacgctg gatattagcg tcgatcgcat cgcgcattat tgttaaggaa tgatcatgag
    50341 tgaccaaccc gttctgttca gcagagtggc ggcttcctgc cgcctgacat tgaaccgtga
    50401 agataaatgt catgctatta acgaagagat gatcgaatct ctcgaccatt atttaaatga
    50461 aattgaaaac gacaccacat tacgattagt cgaattaacg gcaaccggcg ataaattctt
    50521 ttgtgcgggc ggcgatatta aatcgtggtc ggcctattcg ccattagata tgggcagaaa
    50581 atggattaaa cgcggtaatg acgtttttaa tcgcctgcga aacatacccc aattaaccgt
    50641 tgcgaatctc aacggtcata ccatcggcgg tggtattgaa ctggccttgt gctgcgatat
    50701 tcgtattgcc cgccccggcg cgaaattctc taacccggaa gtgatgctcg gcatggtgcc
    50761 tggctggatg ggcattgaac gtgtgcttaa tcaggtcggt ccggtggtcg gtcgccagat
    50821 gctgatgctg ggtaaacgcc tgacggcgca ggaagctcag gcggcgaatt taattgatga
    50881 agtggtagaa aaagaacagg tggaaagctg gatggcgaac cagttagcgc agctggaaaa
    50941 atgcggtccg gtggcactgg cgcatattaa gcaactgatt ctggcactgg aaaataaaca
    51001 tgccgattac ccgcaccagc tactggccgg attaatgtcc gccactcagg attgccagca
    51061 ggcgacgcgc gcctttgccg aaaaaagtag cgtgagcttc cataatcaat aaggaaattg
    51121 tatgcgtaag ataacaaccg ccgaggcact ggcggcgcaa attcaggacg gtgcgactat
    51181 tgctattagc ggtaacggcg gcggtatggt agaagccgac catattctgg ctgctattga
    51241 agcgcgtttc ctgcaaaccg gacaccctcg cgacctgacg ctaatccact cgctgggtat
    51301 tggcgatcgc gactgcaaag gcactaaccg ttttgcgcac gcagagatgc tcaaacgcat
    51361 tattgccggg cactttacct ggtcgcccaa aatgcaggcg ctggtaaaaa ataacacgat
    51421 tgaagcctac tgctttcctg gtggtgtgat tcaagcactg ctacgggaga tcggctccgg
    51481 acgtccgggg ctttttaccc acgttgggct gggatcgttt gttgatccac gcaatggcgg
    51541 cggtaagtcg aatgaatgca ctaccgacga cctggtagaa ctgattgaaa tcgacggtga
    51601 aaccaaactt cgttatcgcc ctttcaaagt ggattacgcg attttgcgtg gcacttatgc
    51661 cgctcctcga ggcaacgtca gccttgaaga agaagcgatt gatatggata gctattccat
    51721 ggcgctggca gcacacaaca gcggcggcaa agtgttcgta caggtacgcg atgtgctgga
    51781 agctggcgcg attgaaccac gccgagtcaa attaccgggc attctggttg atggcatcgt
    51841 tgaacaccgc aaacagccac aaacctatct tggcggttac gacctgacca tcagcggcca
    51901 acatcgccgt ctgagttcta acgacgctat tgaactggtt agtcatccgg tacgtcgcct
    51961 gattgcccgt cgggcagcac gcgaactggt agctggcgct tcaaccaact ttggctttgg
    52021 tattcccggc ggcgttccag gagtagcgcc gcgcgaaggc gttccttacc aaagtttatg
    52081 gctgagcgtc gagcaaggcg tacataacgg catgatgctg gatgatgcgt ttttcggctg
    52141 cgcccgcaac gccgatgcca ttattccatc actcgatcaa tttgagttct acagtggcgg
    52201 agggatcgat atcaccttcc tcggcatggg agagatggat cagtacggta acgtcaacgt
    52261 ctcccatctc aatggcaatc tgattggccc cggcggattt ctcgaaattg cgcaaaatgc
    52321 ccgtaaagtg gtgttctgcg gcacgttcga cgctaaaggc agcaagatcg atataacacc
    52381 agatggcttg catatcgccc agtcaggtca aatccctaaa ctggttacca aggtggaaaa
    52441 aatcactttt agcgccgcct acgcacagca aagtggtcag gaagtgttgt atatcactga
    52501 acgcgcagta ttccagttaa cggcagaagg cgttgaatta attgaaatcg cgccgggtgt
    52561 ggagattgag cgcgacattc tgccgtatat ggctttccgt ccaattatca agcacccacg
    52621 cctgatggaa agtagcctgt ttacgccgat ggaggatgca tgagtcagct tgatgacacc
    52681 atccttgatg cgctgacgca cgttactttc ccaaagggtt ttgcacaggc agagcccgca
    52741 tgggttgtca cggtagacgg tgttaattac ccactctggc aaacagatgc cctggtagtc
    52801 ggcagtggtg cagcaagatt acgtgcggca gttgaactta aacgccgcca gcaaaatgtg
    52861 ctgatcgcta ccgccgggtt atatatgggg acgtcggcat gttctggttc cgataaacag
    52921 acactattta ccgccgctac cgcgggcaac ggcgacaact tcaccaaact ggcagaggca
    52981 ctggcgagcg gtggggcgat ggatcacgac accgcttatg tcgaagcggt aggttctctg
    53041 cacactctag gcgggttgca atatcttggt ctggaattac cggaagatcg ctatggcgcg
    53101 attcttcgtt atcaaaccga ccatgacgaa gccgggcgtg caacctcgtg tgggctgcgt
    53161 acctcaaggt taatggtgaa agtgctgttg gaagaagtac agcgcctcgc cattccagtg
    53221 ttgaccagtg caacagtgat taaactgctg catcagcgtg acgaaaacgg cgaagaccgc
    53281 gtggcagggg caatcctcgc gaccggtcat cgcgcccata acccatgggg gctggcaatt
    53341 gtgactgcgc ccaatgtggt actggcaacg ggcggaccgg gcgagcttta tcgcgacagt
    53401 gtttatccac ataaatgctt cggctcgctg gggctggcgc tggaggaagg tctgacgcta
    53461 accaatctga ccgaaagcca gtttggtata ggcacgccgc gtagcacgtt tccgtggaat
    53521 ttatccggca cctatgtaca ggtgatcccg tatatctatt ccgtggatgc tgagggtaac
    53581 gagtataact tcctcgcgga ttactatcgc accacccagg agctggcttc aaacattttc
    53641 cgtaaaggct atcagtggcc gttccacgcc actcgggtga tggattttgg ctccagcttg
    53701 ttagatatgg cagtagcgca agagcagcaa tcagggcgtc aggtatttat ggatttcaat
    53761 cgcaatcctg aagcggtgcc gggtgacctg ccgttctcat tagatcgact ggacgacgac
    53821 gttcgcgcgt atctggaaaa taacgacgct ctggcaccat cgcccatcga acggctgcaa
    53881 cgaatgaatc cgctgtctat ctcgctgtat aagatgcacg gttacgatct caccacacag
    53941 cctttgcagt ttgccatgaa taatcagcat atgaatggcg gcattgaagt ggatatctgg
    54001 ggacaaacct ccctgcccgg ctgtttcgcc gtgggggaag ttgctggcac acacggcgtc
    54061 actcgccctg gtggtgcagc attgaatgcc gggcaggttt ttgctgttcg tctggcacgt
    54121 tttattggtt gcacgcaaaa gcggtctgtt gagggtgatg tcgcgcagct ggtagcctca
    54181 tctctgaata tagtcagaga agttatcgct caggcactgg caaacgagaa cggaatggcg
    54241 ctggtaacgg tgaaagacaa aatccaggcg cgaatgtctg attacgccgg atttatttgt
    54301 catgccgata aagttcgtca tgccacccgt gacgccctgc tgctaagtga gtttgtacaa
    54361 caacacggtt taaccatcaa tcacgcaggc gaaattgccg aactgtttat gtggcggcat
    54421 atgacgctga cttctgccgc cgtattaacg caattgacgc attatattga cgcaggtggt
    54481 ggcagtcgtg gagcacggat ggttattgat ccacaaggca aatgcctccc acaaactcgt
    54541 cgcggcgcaa aagaagaatg gcgttttcgc tctgaacgtg ctgaagacaa aaatcacaga
    54601 ttaacgattc aatattcgca aggttctttt attaccgaag tgaagtcttt acgtatgcaa
    54661 ccgtgtatta acggtattta ctttgaaaaa actggccaga ctt
//
LOCUS       DAJTMK010000005        53337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTMK010000005 DAJTMK010000000
VERSION     DAJTMK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53337
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.005"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..173)
                     /gene="fadM"
                     /locus_tag="OO135_000237"
     CDS             complement(<1..173)
                     /gene="fadM"
                     /locus_tag="OO135_000237"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5396219.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNIN"
     gene            complement(267..638)
                     /locus_tag="OO135_000238"
     CDS             complement(267..638)
                     /locus_tag="OO135_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5396220.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(789..2660)
                     /gene="ppiD"
                     /locus_tag="OO135_000239"
     CDS             complement(789..2660)
                     /gene="ppiD"
                     /locus_tag="OO135_000239"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5396221.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQVAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDLISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2852..3124)
                     /gene="hupB"
                     /locus_tag="OO135_000240"
     CDS             complement(2852..3124)
                     /gene="hupB"
                     /locus_tag="OO135_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5396222.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(3333..5687)
                     /gene="lon"
                     /locus_tag="OO135_000241"
     CDS             complement(3333..5687)
                     /gene="lon"
                     /locus_tag="OO135_000241"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5396223.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5875..7149)
                     /gene="clpX"
                     /locus_tag="OO135_000242"
     CDS             complement(5875..7149)
                     /gene="clpX"
                     /locus_tag="OO135_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5396224.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVINGQSKPLLIYGKPEAQQASGE"
     gene            complement(7275..7898)
                     /gene="clpP"
                     /locus_tag="OO135_000243"
     CDS             complement(7275..7898)
                     /gene="clpP"
                     /locus_tag="OO135_000243"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5396225.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(8144..9442)
                     /gene="tig"
                     /locus_tag="OO135_000244"
     CDS             complement(8144..9442)
                     /gene="tig"
                     /locus_tag="OO135_000244"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5396226.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9786..10103)
                     /gene="bolA"
                     /locus_tag="OO135_000245"
     CDS             complement(9786..10103)
                     /gene="bolA"
                     /locus_tag="OO135_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5396227.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIV"
     gene            10408..10986
                     /locus_tag="OO135_000246"
     CDS             10408..10986
                     /locus_tag="OO135_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5396228.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            11030..12505
                     /gene="ampG"
                     /locus_tag="OO135_000247"
     CDS             11030..12505
                     /gene="ampG"
                     /locus_tag="OO135_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5396229.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12966..13913
                     /gene="cyoA"
                     /locus_tag="OO135_000248"
                     /pseudo
     CDS             12966..13913
                     /gene="cyoA"
                     /locus_tag="OO135_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
     gene            13935..15926
                     /gene="cyoB"
                     /locus_tag="OO135_000249"
     CDS             13935..15926
                     /gene="cyoB"
                     /locus_tag="OO135_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR5396230.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15916..16530
                     /locus_tag="OO135_000250"
     CDS             15916..16530
                     /locus_tag="OO135_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5396231.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSDFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            16530..16859
                     /locus_tag="OO135_000251"
     CDS             16530..16859
                     /locus_tag="OO135_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5396232.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16871..17761
                     /gene="cyoE"
                     /locus_tag="OO135_000252"
     CDS             16871..17761
                     /gene="cyoE"
                     /locus_tag="OO135_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5396233.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSVIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17910..19268
                     /locus_tag="OO135_000253"
     CDS             17910..19268
                     /locus_tag="OO135_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5396234.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQLPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IR"
     gene            complement(19402..19893)
                     /gene="yajQ"
                     /locus_tag="OO135_000254"
     CDS             complement(19402..19893)
                     /gene="yajQ"
                     /locus_tag="OO135_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5396235.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            20061..20972
                     /gene="panE"
                     /locus_tag="OO135_000255"
     CDS             20061..20972
                     /gene="panE"
                     /locus_tag="OO135_000255"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5396236.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAINCVINPLTAIWNCPNGELRHHPQEIMQVCEEVAAVIERE
                     GHHTSAEDLRHYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            20935..21525
                     /gene="yajL"
                     /locus_tag="OO135_000256"
     CDS             20935..21525
                     /gene="yajL"
                     /locus_tag="OO135_000256"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5396237.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(21579..23027)
                     /gene="thiI"
                     /locus_tag="OO135_000257"
     CDS             complement(21579..23027)
                     /gene="thiI"
                     /locus_tag="OO135_000257"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5396238.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            23233..23475
                     /gene="xseB"
                     /locus_tag="OO135_000258"
     CDS             23233..23475
                     /gene="xseB"
                     /locus_tag="OO135_000258"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5396239.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            23475..24374
                     /gene="ispA"
                     /locus_tag="OO135_000259"
     CDS             23475..24374
                     /gene="ispA"
                     /locus_tag="OO135_000259"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5396240.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLNALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            24399..26261
                     /gene="dxs"
                     /locus_tag="OO135_000260"
     CDS             24399..26261
                     /gene="dxs"
                     /locus_tag="OO135_000260"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5396241.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDDALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            26316..27278
                     /gene="yajO"
                     /locus_tag="OO135_000261"
     CDS             26316..27278
                     /gene="yajO"
                     /locus_tag="OO135_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5396242.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPARGRLTRP
                     WGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSKPGIA
                     APIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(27332..27850)
                     /gene="pgpA"
                     /locus_tag="OO135_000262"
     CDS             complement(27332..27850)
                     /gene="pgpA"
                     /locus_tag="OO135_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5396243.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(27828..28805)
                     /gene="thiL"
                     /locus_tag="OO135_000263"
     CDS             complement(27828..28805)
                     /gene="thiL"
                     /locus_tag="OO135_000263"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5396244.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRFGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(28883..29302)
                     /gene="nusB"
                     /locus_tag="OO135_000264"
     CDS             complement(28883..29302)
                     /gene="nusB"
                     /locus_tag="OO135_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5396245.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(29322..29792)
                     /gene="ribE"
                     /locus_tag="OO135_000265"
     CDS             complement(29322..29792)
                     /gene="ribE"
                     /locus_tag="OO135_000265"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5396246.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(29881..30984)
                     /gene="ribD"
                     /locus_tag="OO135_000266"
     CDS             complement(29881..30984)
                     /gene="ribD"
                     /locus_tag="OO135_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5396247.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVDAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(30988..31437)
                     /gene="nrdR"
                     /locus_tag="OO135_000267"
     CDS             complement(30988..31437)
                     /gene="nrdR"
                     /locus_tag="OO135_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5396248.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            31588..32127
                     /locus_tag="OO135_000268"
     CDS             31588..32127
                     /locus_tag="OO135_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5396249.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            32426..33310
                     /gene="tsx"
                     /locus_tag="OO135_000269"
     CDS             32426..33310
                     /gene="tsx"
                     /locus_tag="OO135_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5396250.1"
                     /translation="MKKTLLAAGVVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(33487..33834)
                     /locus_tag="OO135_000270"
     CDS             complement(33487..33834)
                     /locus_tag="OO135_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5396251.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(33963..34934)
                     /gene="secF"
                     /locus_tag="OO135_000271"
     CDS             complement(33963..34934)
                     /gene="secF"
                     /locus_tag="OO135_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5396252.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(34945..36792)
                     /gene="secD"
                     /locus_tag="OO135_000272"
     CDS             complement(34945..36792)
                     /gene="secD"
                     /locus_tag="OO135_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5396253.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQSVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(36820..37152)
                     /gene="yajC"
                     /locus_tag="OO135_000273"
     CDS             complement(36820..37152)
                     /gene="yajC"
                     /locus_tag="OO135_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5396254.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(37175..38302)
                     /gene="tgt"
                     /locus_tag="OO135_000274"
     CDS             complement(37175..38302)
                     /gene="tgt"
                     /locus_tag="OO135_000274"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5396255.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMD
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(38358..39428)
                     /gene="queA"
                     /locus_tag="OO135_000275"
     CDS             complement(38358..39428)
                     /gene="queA"
                     /locus_tag="OO135_000275"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5396256.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            39521..40102
                     /gene="acpH"
                     /locus_tag="OO135_000276"
     CDS             39521..40102
                     /gene="acpH"
                     /locus_tag="OO135_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR5396257.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALETRFWQFYPRMMAQASHKAL"
     gene            complement(40107..41829)
                     /gene="malZ"
                     /locus_tag="OO135_000277"
                     /pseudo
     CDS             complement(40107..41829)
                     /gene="malZ"
                     /locus_tag="OO135_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(41985..43358)
                     /gene="proY"
                     /locus_tag="OO135_000278"
     CDS             complement(41985..43358)
                     /gene="proY"
                     /locus_tag="OO135_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5396258.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(43434..44753)
                     /gene="brnQ"
                     /locus_tag="OO135_000279"
     CDS             complement(43434..44753)
                     /gene="brnQ"
                     /locus_tag="OO135_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5396259.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGITPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(45160..46455)
                     /gene="phoR"
                     /locus_tag="OO135_000280"
     CDS             complement(45160..46455)
                     /gene="phoR"
                     /locus_tag="OO135_000280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5396260.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRIMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(46513..47202)
                     /gene="phoB"
                     /locus_tag="OO135_000281"
     CDS             complement(46513..47202)
                     /gene="phoB"
                     /locus_tag="OO135_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5396261.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            47392..48594
                     /gene="sbcD"
                     /locus_tag="OO135_000282"
     CDS             47392..48594
                     /gene="sbcD"
                     /locus_tag="OO135_000282"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5396262.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            48591..51734
                     /gene="sbcC"
                     /locus_tag="OO135_000283"
     CDS             48591..51734
                     /gene="sbcC"
                     /locus_tag="OO135_000283"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5396263.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            51860..53044
                     /gene="araJ"
                     /locus_tag="OO135_000284"
     CDS             51860..53044
                     /gene="araJ"
                     /locus_tag="OO135_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5396264.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(53187..>53337)
                     /locus_tag="OO135_000285"
     CDS             complement(53187..>53337)
                     /locus_tag="OO135_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5396265.1"
                     /translation="MSNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAAW
                     LWPQE"
BASE COUNT        12704 a        14285 c        13379 g        12969 t
ORIGIN      
        1 atattgatat tgaccacgac gaaggcgatg ttatgggccg tcatccactg aaaactgtcg
       61 ctattttcca acccatccca gcgggcttct tcgagaaatt caaggtagcg ggcgttgttg
      121 acgtgctggt aaacgtcgag atgatatcca cgaactttga tttgtgtttg catagcgcaa
      181 taaccttacg ttgttgttat aagtacagag gtcataactg gtatgacctc ttcagtctgg
      241 caaaaaattg ccactatgca aattaattac agggttaata ccgccagatt acgttccacc
      301 agcgaattgc ccatccccgg cacctgcttt agatcctcca ctgttttaaa cggaccgtac
      361 tcttcgcgat aactgacaat cgcctgcgct ttcttcaggc caacgccatt catcgcgcgg
      421 gctagctctt ccgcgctggc attattaatg ctgacccggg tgccttcttc gtcactggct
      481 ttcgccggta ctgctgcttt actttgtgct gcaggagctt ccgctttggt ttctaccgcc
      541 gtcggtttcg ccacagaagc tgccgccagc gcgctatgag acattccggc acaggccagg
      601 gacagggtaa tgagcagtgc tttaattccg tgtttcatac tgttttctcc ttgtttgtta
      661 acagtggaat caccttaacg gcagtgaaaa cagtaaacaa atggcaatgt tcagaaatgg
      721 aaaaggccgc gaaagcgacc ttttcttgtt acagctgcaa cgcatttttt tgcgaggcgt
      781 cttcaggatt attgctgttc cagcgcatcg ccaattttga ttttcgcctc tttacgcagg
      841 ttactcatca gagcttcaaa gacgatttgt gcgttgttct gggtgatacc ctgcaccatc
      901 gcttttttct gatcttccgg cattgaacct tgtttcactt catccagcgc cagcagaacc
      961 acattacctt gcatatcggt cgccataccg tagctcggtt tgtctttcgc tggcagtggc
     1021 agtgcaaacg ccgcctggct aatcaggtca cgaccggaac ggcttaaggt tttcggctcg
     1081 ccaaatttca gaccggcagc ctgcatagct tccgcacctt tgccggcttt caaatcaacc
     1141 agcagtttct cagcatccac tttcgcctgt tgttcagctt tgttgtgctg aaccagtgcc
     1201 ttaacttgtt cctgaacatc tgccaacggt ttcaccgctt ccggtttgtg ctcgctgatg
     1261 cgcagcacga atgcacggtc gccgtctacg gtgatgatgt cagagttgat gcccggcgcg
     1321 ccgttttcac ctaccagacc gccgttaaag atagcgtcgg caaccggctt gaagttcaac
     1381 tcttccggca ggttatcttt gctgaaccaa cccgtctgag tggctttaac gccggcaact
     1441 tgctctgcac cggccagaga ctcggtgtcg ttgcttgccg catcgctcac tttctgctgc
     1501 agcgcatagt acgcatcgag ggctttttcg tgtttcactt tcgccgcaat gtcgtcacgt
     1561 acttcgtcta acgatttcac tttcgctggc tgaatgtcgt ccagacgtac aatcaggaaa
     1621 ccgaccgaag atttgatgac accagacagt tggccttttt ctttcagacc agcatttttc
     1681 agttcgtcgg ggatagtggc atcttctaac caacccatat cgccgccgtt acgggcagag
     1741 ataatatcgg cagatttttc tttggctaat gcagcaaaat caccgccttt attcagctca
     1801 tcaagtaccg ctttcgcttc atcttcagtt ttggtctgga tgatgctgta gcgggtacgc
     1861 tgcggctggg tgaattgatc ctgatgctga tcgtagtagc tctggatatc cgcatcgcta
     1921 accggttgct gcatcgttgc ggcatccagc ttgatgtaac tcacgcggaa ttgttccggc
     1981 gtcatgaaat tgtttttgtt ttgttcgtag tagctggcaa tctcctgttc ggtcacaggc
     2041 tgcttcgccg ccagcgcgtt aacatcgata gtcgcctcac gcaccacgcg ttgttgcgcg
     2101 accagtgccg ccagctcgtc ggtttcacct ttcagcataa aatcggtacc ggcaacaccg
     2161 ttaatcagct gttgggtagt gagctggtta cgcagcgcct gggcgtactg atcggcggtc
     2221 atccccatct ggttgaggat accgttatag cggctgttat caaatttgcc gtcaacctgg
     2281 aaggctgggg tcgcgaaaat cgcctgttta acctgctcat cactgatacc cagtttcagc
     2341 tcacgagcgt actgatccaa cagcgcctcg tcgatcagac gattcagcac ctgttgacgc
     2401 agggttttca tatagccttc attcgctgcc agctcggagt attgatcgcc cagctgttgc
     2461 tgcatgcgat tacgctcgct gttaaaggcg ttctcgaact gcccacggct gatttcctgg
     2521 tcattcactt ttgcggcgta gttattgcct ccgccaatca ggtaaccact cacgccggtc
     2581 aatatgaacg acacgataat gataccgaaa ataatcttga gcacgagact gtttgcagcc
     2641 gtgcgtaagc tgtccatcat ggtgtaacaa cactccgctg tagatgactg tgtacctcac
     2701 gcaacgtatc gatgcgcgca aaggcctatt gtgacaagaa acgggggcaa ttgtcagccc
     2761 acaagacgca gaaagtcacc gaatacaaat aaaaaaggca catcagtaga tgcgcccttg
     2821 aacttcgtca catccccacc ggggacaacg cttagtttac cgcgtctttc agtgctttac
     2881 ctgcacggaa gctcggtact ttagcagcag cgatggtgat ctctttaccg gtctgcgggt
     2941 tgcggccagt acgggcagca cgctctttaa cggcaaaagt accaaaacct accagtgcta
     3001 catcatcccc ttctttcaga gattcagtta cggaagcaat aatagcatct aacgcacggc
     3061 cagccgcagc tttagagata tcagcccctg cagcaatctt gtcgatcaat tgagatttat
     3121 tcactcttct cttcctcttt ataatttata tcgcacttga atccttcaag gtacgaacgc
     3181 gcagcagtta tatcaggcca gccatcccct tacaacaccc cttaataagg gcaagcccga
     3241 tccgccatct aacttagcga gacaaaaaaa ggctggcaag cccgaattag cctgccagcc
     3301 ctgtttttat tagtgcattt tgcgcgaggt cactattttg cagtcacaac ctgcatgcca
     3361 gacggttcat tttgcagcgc cagagtcaga acttcctcaa tgcgcttcac aggatgaatg
     3421 tccagatcgg caattacgtt gtcaggaatc tcttccagat cgcgtttatt ttcgaacgga
     3481 attagcactg ttttaatccc gccgcgatgc gctgccagga gtttttcttt caaaccaccg
     3541 atcggcagta cctgaccacg cagagtgatc tcaccggtca ttgccacatc ggcacgaacc
     3601 gggttaccgg tcaggcaaga aaccagcgcg gtgcacatag caataccggc actcggacca
     3661 tctttcggcg tcgcaccttc cggtacgtgg acgtggatgt cacgtttttc gtaaaaatca
     3721 gggttgatcc ccagtttttc cgcacgcgca cgaaccaccg ttaacgccgc ctgaatagac
     3781 tcctgcatca cttcgccgag cgaaccggta taggtcagtt tgcctttacc cggaacacat
     3841 gcggtttcaa tggtcagcaa gtcaccgccc acttccgtcc acgccagacc ggttacctga
     3901 ccgacacagt tttcgttatc agcgcgacca tagtcgaaac gctgaacacc gaggtagtca
     3961 tgcaggttat cgccgttaat ttcgatatgt tttaatgact tatcgagcag taactgctta
     4021 accgctttgc ggcacagttt ggagatttca cgctccagac cacgcacgcc cgcctcacgg
     4081 gtgtagtaac gaataatgcc gataatggcg ctatcgtcga cggtcagctc accttttttc
     4141 agtgcattac gttcaatctg cttcggcagc aggtgacgtt tggcgatgtt cagtttttca
     4201 tcttcggtat aaccggagag gcgaatcact tccatacgat ccagcagcgg tgccggaatg
     4261 ttcatggagt tcgacgtcgc gacaaacatc acgtcgctga gatcgtaatc cacttccagg
     4321 tagtggtcgc tgaacgctac gttctgctct ggatccagca cttcaagcag tgcagaggcc
     4381 ggatcgcctc gcatgtcaga agacattttg tcgatctcat cgagcaggaa cagcgggttt
     4441 ttcacgccca ctttcgccat tttctggatc agtttacccg gcatagaacc gatgtaagta
     4501 cggcggtgac cacggatttc cgcttcatca cgcacgccgc ccagcgccat acggacatat
     4561 ttacgcccgg tggctttggc aatggactga ccaagagagg ttttacctac ccccggcggc
     4621 cctaccaggc agaggatcgg tcccttgatt ttgttgacac ggctttgaac cgcaaggtac
     4681 tcaaggatgc gatctttcac gcgctccaga ccataatggt cggtatcaag gatttcctgc
     4741 gcctgacgca ggtctttttt gaccttgctg cgcgcattcc acggtacctg taccatccag
     4801 tcgatataac cacgcactac ggtcgcttct gccgacatcg gagacatcat tttcagcttc
     4861 tgcaactctg cttccgcttt ctcttttgcc tctttcggca tcttcgccgc gtcgattttg
     4921 cgcttcaggg cttcgttttc gtccggcgcg tcgtccattt caccgagttc tttctgaata
     4981 gctttcattt gctcgttcag atagtactca cgctgggatt tctccatctg ctttttaacg
     5041 cggttgcgaa tgcgtttctc aacctgcagc agatcgattt ccgattccat cattgccatc
     5101 agatattcca gacgttcgtt aacgtcggac atctccagaa cggactgttt gtcagccagt
     5161 ttcagtggca tatgtgcagc aatggtatcc gccagacgcg ccggatcgtc gatgctattc
     5221 agcgacgtca gcacttctgg tgggattttt ttgttcagct tgatgtagcc ttcgaactgg
     5281 ctgattgcag tacgcaccag cacttcctgt tcccgctcat caatggtcgg cgactccaga
     5341 tactccgcct tcgcagaaaa gtgttcgcca ttgtcagaga gcgcagaaat acgcgcgcgc
     5401 tgtaacccct cgaccagcac tttgacggtg ccgtcaggca gtttcagcat ctgcaatata
     5461 gaggccacgg tcccgacggt gaaaagatcg tttacacccg gctcatccgt tgaagcttct
     5521 ttctgcgcga ccagcataat ttttttatca tggtccatcg ccgcttccag acaacggata
     5581 gatttttccc gcccgacaaa taaggggatg accatgtgcg gataaaccac cacatcgcgc
     5641 agcggcaata cggggatttc aatgcgttca gaacgctcag gattcataga gctctctctt
     5701 agtttaattt ccgccaggta atcagatgac acgactgtgc ttcacgccat ctattaacat
     5761 gtacgtcagt atatggggat gtttccccca cattcaacgc cgagaataga ggaaaaatta
     5821 aaggggagat aaaatccccc ctttttggtt aactaattgt atgggaatgg ttaattattc
     5881 accagatgcc tgttgcgctt ccggcttacc ataaatcagc aacggtttgc tttgaccatt
     5941 aattaccgac tcgtcgataa ccactttttc gacatcttcc atggacggca gatcgtacat
     6001 ggtatcgagc agtgcggctt ctacgatgga acgcaggcca cgggcaccgg ttttacgcgc
     6061 catcgctttc ttagcgatag catccagcgc ctcgtcacgg aattccagat ccacgccttc
     6121 cagattaaac agcgcctgat actgcttggt cagggcgttt ttcggctctt tgaggatctg
     6181 aatcagagct tcttcgctca gttcattcaa cgttgcgaca accggcagac gaccaataaa
     6241 ctcagggata agaccaaact tgatcagatc ttccggttca acctgcgcca gcagctcgcc
     6301 ttcgcttgct ttgtcggact tcgcttttac cgtcgcgcca aaaccaatgc cggaaccggt
     6361 ttctacacgg tgggaaatca ctttatccag accggcaaac gcaccgccac aaataaacag
     6421 gatcttggag gtatcaacct gcaaaaattc ctgctgcgga tgcttacgcc cgccctgcgg
     6481 tggaacggca gcaacagtac cttcgatcag tttcaacagt gcctgctgta cgccttcacc
     6541 ggaaacgtca cgggtaatag acgggttgtc cgacttacga gaaatcttgt cgatttcatc
     6601 gatgtagaca ataccacgct gtgctttctg gacatcgtag tcgcatttct gcaacagctt
     6661 ctgaatgatg ttttcaacgt cttcacccac ataaccggct tcggtcagtg tagtcgcgtc
     6721 ggccatggtg aacggaacat ccagcaggcg cgccagcgtt tcagccagca gcgttttacc
     6781 ggaaccggtc ggaccgatca gcagaatgtt acttttgccc aactcgacac cattgctggt
     6841 atcgccgttg cgcagacgtt tgtaatggtt gtataccgcg accgccagca cttttttcgc
     6901 ctgttcctgg ccgataacgt aatcgtccag gtggttgcga atttcatgcg gcgtcggtag
     6961 cgcactgcgt tcacgatgcg gtgcaacttc tttaatctct tcgcgaatga tgtcgttaca
     7021 taaatcaaca cattcgtcgc agatatacac ggatggaccg gcaatcagct tgcgcacttc
     7081 atgctggctt ttgccgcaaa aagagcaata cagcaatttg cctgagccat ctttgcgttt
     7141 atctgtcatg ggtcaaaacc tcttctttgt tctttgtgcc gcacacgacg acgcaaatgc
     7201 cattctcagg cgcaagccgc ttacagcgtt gtgccgccct ggataagtat agcggcacag
     7261 ttgcgcctct ggcatcaatt acgatgggtc agaatcgaat cgaccagacc gtattccacc
     7321 gcttcagggg cggaaaggaa gcgatcgcgc tcggtatcac gttcaatctg ttctaatgat
     7381 tgacccgtat gaagcgccat aagttcattc atgcgccctt taactttcag aatttcacgg
     7441 gcatgaattt cgatatcggt cgcctggccc tggtagccgc ccaacggttg gtgaatcatc
     7501 acgcgcgaat tcggcaggca aaaacgttta ccttttgccc ctgcggtcag caagaaagcg
     7561 cccatcgagg ccgcctggcc catacagatg gtgctgacat caggcttgat aaactgcatg
     7621 gtgtcataga tagacatccc ggcagtgatc accccgcctg gggagttaat gtacagatag
     7681 atatcttttt ctgggttttc cgcttccagg aacagcatct gcgccacaat caggttagcc
     7741 atgtggtctt caacctggcc agtcagaaaa atgacgcgtt ccttaagtag acgagaatag
     7801 atatcaaaag agcgctcacc gcgtgaggtc tgttcaatga ccatcggcac cagcgccata
     7861 tggggtgcaa agttatctcg ttcgccgctg tatgacattt ccgtctcctg gataaaattg
     7921 aaaaaacctg ctgtaccgat tgtaacctta tggacggatt atcagctagt ccctgtgtta
     7981 tgggtacggc atcaccatat ttcaagcata acaatctttt gttgttacgc taacaccgaa
     8041 agaggctttt cgcacggttc catgcaaaat tcatgacaaa aaaaagccca ccacctggcg
     8101 gtgacgggcc tttgtgcgaa tttagcgcgt tatgctgcgt aaattacgcc tgctggttca
     8161 tcagctcgtt gaaagtggtt tctttttcag tcactttcgc tttcgccagt acagcttcaa
     8221 cagcctgttc ttccagagca acattgcgca tgttgtccat cagttctttg tttttgctgt
     8281 agaactcgat aacttctttc ggatcttcgt acgcagaagc catctcttcg atcaggcctt
     8341 tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat aacttcgccc agcagcaggc
     8401 caacaactac gcggcgttta gcctgttctt cgaacagttc gcgcggcagt tccagagctt
     8461 gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg cagaacgtcg atttcgctgt
     8521 cgatcagcgc agccggtacg tcgatgtcgt tagcttttac cagaccttcg atcgcctgag
     8581 acttaacgcg gttacggatg gcgctcttca gctcgcgctc catgttttta cgcacttcag
     8641 cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg tttgatgaat tctgcagtca
     8701 gttccggcag ttcacgctct tcaactttct tcaggttgat agcgaatttc gctgctttac
     8761 ctttcaggtt ttctgcgtgg tattcttccg ggaaggtcac gtcgatggtg aactcttcgc
     8821 cagctttgtg gcctttgata ccgtcttcaa agcccgggat catacgaccc tggcccatcg
     8881 ccagtacgaa atcagacgct ttaccgcctt cgaactcttc gctgtctaca gaaccggtga
     8941 agtcgatggt tacgcggtct tctgcttcaa cagcgccgtc tttttctttc caggtcgcct
     9001 gctgtttacg cagagtatcc agcatgccgt caacgtcagc gtcggtcact tcaacgatcg
     9061 gtttttcaac ttcgatcgct tccagaccct gcagttcaac ttccggataa acttcaaact
     9121 ctacagagta agtgaagtct tcacccagct tgtattcgcc cggaacataa gtcggtgcgc
     9181 cagccggatt gattttttct ttaatgatgg cgtcaatgaa gttacggctc atcaggtcac
     9241 ccagaacgtc ctggcgtaca gacgcgccat aacgctgagc aacgatattc attggcactt
     9301 tgcctttgcg gaagccgtca atacgtactt ttttcgcaac gttgaccagc tcgcttttaa
     9361 cagcggtctc gatgctatca gcagcgatag taatcgttac acggcggcca aggccttgag
     9421 tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat cacagtgctc ggtcaactct
     9481 taccccgcaa gcacaggttg ttggcttcgc ggaaccggga tgttcttata atcaatcaca
     9541 ttccctgtta ccagaatcat cccgaaagca ttcaaatagg acacggcatt atagcggcat
     9601 cacttttatg agtcgagaac ggttattgcg cgctgtcgcg tattttttca caattcccgg
     9661 gcaattttcg tctgaatgct gggcaaaaag cgcaaaatcc agacaaaaca aaacggcccg
     9721 caggccgttt ttcataaaat ctttagcaac atactgaaaa agcgccgaca gttgcaaatg
     9781 cgtttttaca cgatgcttcc tgctccacga cagggaggag aggcaaagac ggtgtcctgc
     9841 aacccttccc actccttaat agtgtaagta tgcagagcca gcgcatgaac ggtagtagag
     9901 agttcctccg ctaaagtact gtaaatcatt cgatgacgat tcagaaaacg ttcacccgta
     9961 aaacgatcgc tgaccagcac aactttaaaa tggctttcag agccggctgg gacattgtga
    10021 cgatagcttt catccactac ttcgaggaat acgggttgga acgccgccct taatttttct
    10081 tctatccgct cacgtatcat catgaaatat ctcctccgac aacgctgaga tgtcatccat
    10141 ccctttaaat actagccgct tttaccgttt ccattgcagc ttaacaacaa atatttaggt
    10201 ttcgtcctgg tttttccgtc aagcgtatga agtttactga tttaaagatt gaaacacagg
    10261 cctcgcatca gcgtttcttt taccaggggt gattatttcg tcaacatgat gaatttacat
    10321 gccttaccca cttcccctcg ccgttggcaa tgttatgatg gcgggaattt ttaccaaaca
    10381 cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa tcctcttccc gttagttgct
    10441 ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta ttgaagtttc cccgacgatt
    10501 acactgccac agcaggatcc aagcctgatg ggcgtcaccg taagcattaa tggtgccgat
    10561 cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata atcaaatcgt taccctgacc
    10621 gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg aaaaacagat gaccgcgcgt
    10681 ggttacatgg ttggtccgaa tggcccggtt aatctgcaaa tcatcgttag ccaactgtat
    10741 gctgacgtgt cccagggcaa cgtgcgctac aacatcgcta ccaaagcgga tatcgccatc
    10801 atcgctaccg cgcagaatgg caacaaaata accaaaaact atcgtgccag ctacaacgtt
    10861 gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg cggttaacag tgtgctgacc
    10921 gataccatcg ctgatatgtc tcaggacacc agcattcacg aattcatcaa gcagaacgcg
    10981 cgttaatttc tgccctctgg cccggtgcaa accgggcctg tagacgccca tgtccagtca
    11041 atatttacgt atttttcaac agccgcgttc agccatattg ctgatcctgg gttttgcttc
    11101 cgggctaccg ctcgccctga catccggtac cttacaggcc tggatgacgg tcgagaatat
    11161 tgatctcaaa accattggtt tcttctctct ggtaggccag gcttacgttt ttaaattcct
    11221 ctggtcaccg ctgatggacc gctacacgcc cccatttttt gggcggcggc gcggttggct
    11281 gctcgccacg caaatcctgt tattagtcgc cattgcggcg atgggttttc tcgaacctgg
    11341 cacccaactc cgctggatgg cggcactggc ggtggtgatc gctttttgct cggcctccca
    11401 ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg gcagaagaac gtggtgcggg
    11461 cgcggcaatc agcgtgctgg gttaccgttt agggatgctg gtttccggcg gcttagccct
    11521 gtggctggca gataaatggc tgggctggca gggcatgtac tggttgatgg cggcgctgtt
    11581 gatcccttgt attatcgcga cgttgcttgc accagaaccg accgacacaa ttcctgtgcc
    11641 aaaaacgctg gaacaagcgg ttgttgcacc tctgcgagat ttctttggtc gcaataatgc
    11701 ctggcttatt ttgcttctta tcgtgctgta taagctgggc gacgcattcg ccatgagcct
    11761 gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg ggtgaagtag gcgtggttaa
    11821 caaaacgctt ggcttactgg cgaccattgt gggcgcattg tacggtggga ttttgatgca
    11881 gcgcctgtcg ctgttccggg cgctgctgat tttcggcatt ttacaaggtg cgtctaacgc
    11941 tggttactgg ctgctgtcga ttactgataa gcatctctac agcatgggcg cagccgtctt
    12001 tttcgaaaac ctctgtggcg ggatgggcac atcagccttt gtcgcgctgt taatgacgct
    12061 gtgtaataag tcattttccg ctactcaatt tgccctgctc tcagcgcttt ccgctgtagg
    12121 acgagtttat gtcggccccg tggcgggttg gtttgttgaa gcacacggct ggtcgacatt
    12181 ctatctattc tccgtcgccg ctgccgtacc ggggcttatt ttgctgctgg tttgccgcca
    12241 gacgcttgaa tatacacgag taaatgacaa ctttatctcc cgtaccgaat atccggcagg
    12301 ttatgccttt gccatgtgga cactggcggc gggcgtcagc ctgttggccg tgtggttact
    12361 gctgttgacg atggacgcgc tggatttgac gcacttctct ttcctgcctg ctctgctgga
    12421 agtcggggtt ttagtcgccc tttctggcgt cgtgcttggt ggtttgctgg attatctggc
    12481 tctacgaaaa acgcatctaa cgtaatctgt aaatattatt tagctgtagt aatcactcgc
    12541 cgtaattatt acggataaat aaacatcagt atcgcttaat aatatttgtt cttttgtgcg
    12601 gcttagcgtt tggaaattat tgacgcaatt tatgaattct attttttctt acacgattca
    12661 gctaacgagt ctttattttc cccccaccca attgtcacca taatgataat tatttgttaa
    12721 acaattattt tattccagat tggttatacc aattgcccat ccaaactcct tctgcactcc
    12781 cctttcgtta taacgccctt ttgcaacagc ttcttaaaat caacttgata tgttttgcaa
    12841 catatgtgac ctgacagcca aatccaagta acagtaattt aatcatgttt acagtaatgt
    12901 aaccttcccg taaaatgccc acacacttta aacgccacca gatcccgtgg aattgaggtc
    12961 gttaaatgag actcaggaaa tacaataaaa gtttgggatg gttgtcatta tttgcaggca
    13021 ctgtattgct cagtggctgt aattctgcgc tgttagatcc caaaggacag attggtctgg
    13081 agcaacgttc actgatactg acggcatttg gcctgatgtt gattgtcgtt attcccgcaa
    13141 tcttgatggc tgttggtttc gcctggaagt atcgtgcgag caataaagat gctaagtaca
    13201 gcccgaactg gtcacactcc aataaagtgg aagctgtggt ctggacggta cctatcttaa
    13261 tcatcatctt ccttgcggta ctgacctaga aaaccactca cgctcttgag cctagcaagc
    13321 cgctggcaca cgacgagaag cccattacca tcgaagtggt ttccatggac tggaaatggt
    13381 tcttcatcta cccggaacag ggcattgcta ccgtgaatga aatcgctttc ccggcgaaca
    13441 ctccggtgta cttcaaagtg acctccaact ccgtgatgaa ctccttcttc attccgcgtc
    13501 tgggtagcca gatttatgcc atggctggta tgcagactcg cctgcatctg atcgccaacg
    13561 aacccggtac ttatgacggt atctccgcca gctacagcgg cccgggcttc tcaggcatga
    13621 agttcaaagc tattgcaaca ccggatcgcg ccgaattcga ccagtgggtc gcaaaagcga
    13681 agcagtcgcc gaacaccatg tctgacatgg ctgcgttcga aaaactggcc gcgcctagcg
    13741 aatacaacca ggtggaatat ttctccaacg tgaaaccaga cttgtttgct gatgtgatta
    13801 acaagtttat ggctcacggt aagagcatgg acatgaccca gccagaaggt gagcacagcg
    13861 cacacgaagg tatggaaggc atggacatga gccacgcgga atccgcccat taaaggggtt
    13921 gaggaagaat aaagatgttc ggaaaattat cacttgatgc agtcccgttc catgaaccta
    13981 tcgtcatggt tacgatcgct ggcattattt tgggaggtct ggcgctcgtt ggcctgatca
    14041 cttacttcgg taagtggacc tacctgtgga aagagtggct gacctccgtc gaccataaac
    14101 gcctcggtat catgtatatc atcgtggcga ttgtgatgtt gctgcgtggt tttgctgacg
    14161 ccattatgat gcgtagccag caggctcttg cctcggcggg cgaagcgggc ttcctgccac
    14221 ctcaccacta cgatcagatc ttcaccgcgc acggcgtgat tatgatcttc ttcgtggcga
    14281 tgcctttcgt tatcggtctg atgaacctgg tggttccgct gcagatcggc gcgcgtgacg
    14341 ttgcgttccc gttcctcaac aacttaagct tctggtttac tgttgttggt gtgattctgg
    14401 ttaacgtttc tctcggcgtg ggcgaatttg cgcagaccgg ctggctggcc tatccaccgc
    14461 tatcgggaat agagtacagt ccgggagtcg gtgttgatta ctggatatgg agtctccagc
    14521 tatccggtat aggtacgacg cttaccggta tcaacttctt cgttaccatt ctgaagatgc
    14581 gcgcaccggg catgaccatg ttcaagatgc cagtatttac ctgggcatca ctgtgcgcga
    14641 acgtactgat tattgcttcc ttcccaattc tgacggttac cgtcgcgttg ttgaccctgg
    14701 atcgctatct gggcacccat ttctttacca acgatatggg tggcaacatg atgatgtaca
    14761 tcaacctgat ttgggcctgg ggccacccgg aagtttacat cctgatcctg cctgttttcg
    14821 gtgtgttctc cgaaattgcg gcaaccttct cgcgtaaacg tctgtttggt tatacctcgc
    14881 tggtatgggc aaccgtctgt atcaccgtgc tgtcgttcat cgtttggctg caccacttct
    14941 ttacgatggg tgcgggcgcg aacgtaaacg ccttctttgg tatcaccaca atgattatcg
    15001 ccatcccgac cggggtgaag atcttcaact ggctgttcac catgtatcag ggccgtatcg
    15061 tgttccattc tgcgatgctg tggaccatcg gttttatcgt cactttctcg gtgggcggga
    15121 tgactggcgt gctgctggca gtaccgggcg cggacttcgt tctgcataac agcctgttcc
    15181 tgattgcgca cttccataac gttatcatcg gcggcgtggt cttcggctgc ttcgcaggga
    15241 tgacctactg gtggcctaaa gcgttcggtt tcaaactgaa tgaaacctgg ggtaaacgcg
    15301 cgttctggtt ctggatcatc ggcttcttcg ttgcctttat gccgctgtat gcgctgggct
    15361 tcatgggcat gacccgtcgt ttgagccagc agattgaccc gcagttccac accatgctta
    15421 tgattgcagc tagcggtgca gtactgattg cgctgggtat tctctgcctc gttattcaga
    15481 tgtacgtttc tattcgcgac cgcgaccaga accgtgacct gactggcgac ccgtggggtg
    15541 gccgtacgct ggagtgggca acctcttccc cgcctccgtt ctataacttt gccgttgtgc
    15601 cgcacgttca cgagcgtgat gcattctggg aaatgaaaga gaaaggcgaa gcgtataaaa
    15661 agcctgacca ctatgaagaa attcatatgc cgaaaaacag cggtgcaggt atcgtcattg
    15721 cagctttctc caccatcttc ggtttcgcca tgatctggca tatctggtgg ctggcgattg
    15781 ttggcttcgc aggcatgatc atcacctgga tcgtgaaaag cttcgacgag gacgtggatt
    15841 actacgtgcc ggtggcagaa atcgaaaaac tggaaaacca gcatttcgat gagattacta
    15901 aggcagggct gaaaaatggc aactgatact ttgacgcacg cgactgccca cgcgcacgaa
    15961 cacgggcacc acgatgcagg cggaaccaaa atcttcggat tttggatcta cctgatgagc
    16021 gactgcattc tgttctcaat cttgtttgct acctatgccg ttctggtgaa cggcaccgca
    16081 ggcggcccga caggtaagga cattttcgaa ctgccgttcg ttctggttga aactttcttg
    16141 ctgttgttca gctccatcac ctacggcatg gcggctatcg ccatgtacaa aaacaacaaa
    16201 agccaggtta tctcctggct ggcgttgacc tgtttgtttg gtgccggatt tatcgggatg
    16261 gaaatctacg aattccatca cctgattgtt aacggcatgg gtccggatcg cagcgacttc
    16321 ctgtcagcgt tctttgcgct ggtcggcacg cacggtctgc acgtcacttc cggtcttatc
    16381 tggatggcgg tgctgatggt acaaatcgct cgtcgcggcc tgaccagcac taaccgtacc
    16441 cgcatcatgt gcctgagcct gttctggcac ttcctggatg tggtttggat ctgtgtgttc
    16501 actgttgttt atctgatggg ggcgatgtaa tgagtcattc taccgatcac agcggcgcgt
    16561 cccatggcag cgtaaaaacc tacatgacag gctttatcct gtcgatcatt ctgacggtga
    16621 ttccgttctg gatggtgatg acaggggctg cctctccggc cgtaattctg ggaacaatcc
    16681 tggcaatggc agtggtacag gttctggtgc atctggtgtg cttcctgcac atgaatacca
    16741 aatcagatga aggctggaac atgacggcgt ttgtcttcac cgtgctaatc atcgctatcc
    16801 tggttgtagg ctccatctgg attatgtgga acctcaacta caacatgatg atgcactaag
    16861 agcggcggtt atgatgttta agcaatacct gcaagtaacg aaaccaggca tcatctttgg
    16921 caacctgatc tcggtgattg ggggattcct gctggcctca aagggcagca ttgattatcc
    16981 cctgtttatc tacacgctgg ttggggtgtc actggttgtg gcgtcgggtt gtgtgtttaa
    17041 caactacatc gacagggata tcgacagaaa gatggaaagg acgaagaatc gggtgctggt
    17101 taaaggcctg atctctcctg ctgtctcgct ggtgtacgcc accttgctgg gtattgctgg
    17161 ctttatgctg ctgtggtttg gcgcgaatcc gctggcctgc tggctggggg tgatgggctt
    17221 tgtggtttat gtcggcgtct atagcctgta catgaaacgc cactcagtct acggcacatt
    17281 gattggttcg ctctccggcg ctgcgccgcc ggtgattggc tactgtgcag taaccggcga
    17341 gttcgatagc ggcgcagcga tcctgctggc tatcttcagc ctgtggcaga tgcctcactc
    17401 ctatgccatc gccattttcc gctttaagga ttaccaggcg gcaaacattc cggtattgcc
    17461 agtggtaaaa ggcatttcgg tggcgaagaa tcacatcacg ctgtatatca tcgcctttgc
    17521 cgttgccacg ctgatgctct ctcttggcgg ttacgctgga tataaatatc tggtggtcgc
    17581 cgcggcggtt agcgtctggt ggttaggtat ggctctgcgc ggttataaag ttgctgatga
    17641 cagaatctgg gcgcgcaagc tgttcggctt ctctgtcatc gccatcactg ccctctcggt
    17701 gatgatgtcc gttgatttta tggtaccgga ctcgcatacg ctgctggctg ctgtgtggta
    17761 acaaaacctc tctattaaaa aggtgctacg gcaccttttt tcttagcatt agaaacatat
    17821 ccctctcgaa atatttacta aaaaatccgc atgtttaccc cattcgtttg ccgctttaca
    17881 ctagtcgcga atttaaaaca gaggtggtaa tgaacgatta taaaatgacg ccaggtgaga
    17941 ggcgcgcgac ctggggttta gggaccgtat tctcgttgcg catgctgggc atgtttatgg
    18001 ttctgccggt tctgaccacg tatggcatgg ctctgcaagg tgccagcgaa gcattaatcg
    18061 gtattgccat tggtatttat ggtctgactc aggccgtttt tcagcttccg tttggcctgc
    18121 tttccgaccg tattggtcgc aaaccattaa ttgtcggtgg gctggcggtg tttgccgccg
    18181 gtagcgttat cgctgcgctc tctgactcca tctggggaat tattctgggc cgggcgctac
    18241 aaggctccgg tgcgattgcc gctgccgtta tggcgctgct ttccgatctc acgcgcgaac
    18301 aaaaccgcac caaagcaatg gcgtttatcg gcgtgagctt tggcattacc tttgccattg
    18361 cgatggtgct tggcccgatc atcactcaca aacttgggct gcacgcgctg ttctggatga
    18421 tcgctattct ggcaacgacc ggcattgcgt tgaccatttg ggttgtgccc aacagtagca
    18481 ctcacgtact taatcgtgag tccggaatgg tgaaaggcag tttcagtaaa gtgctggcgg
    18541 aaccgcggct gctgaaactc aactttggca ttatgtgtct gcatattttg ctgatgtcga
    18601 cgtttgttgc cctgcccgga caactggctg atgcagggtt cccggcggct gaacactgga
    18661 aggtctatct ggcgacaatg ctaatcgcct ttggctcggt cgtgcctttc attatctacg
    18721 ctgaagttaa gcgcaaaatg aagcaagtct ttgtcttctg cgtcgggttg atcgtggttg
    18781 cggaaattgt gttgtggaac gcgcaaacgc agttctggca actggtggtc ggcgtgcagc
    18841 ttttctttgt agcgtttaat ttgatggaag cccttctgcc ttcacttatc agtaaagagt
    18901 cgccagcagg ttacaaaggt acagcgatgg gtgtttactc caccagccag tttcttggcg
    18961 tggcgattgg cggttcactg ggcggctgga ttgacggcat gtttgacggt cagggcgtat
    19021 ttctcgctgg cgcaatgctg gccgcagtgt ggctggcagt cgccagtacc atgaaagaac
    19081 cggcgtatgt cagcagtttg cgcattgaaa tcccggcgaa cattgccgca aacgaggcgt
    19141 taaaagtgcg tttgctagaa actgaaggca tcaaagaagt gttgattgca gaagaagaac
    19201 attcagctta tgtgaaaatc gacagcaaag tgacgaatcg ctttgatgtt gaacaggcaa
    19261 ttcgctaggc ataaaagcga ggcgaaattg cctgctgcgc tacgctcatc aggcctacta
    19321 agctcctgat ttacaagcct ttgtaggccg aaccaggcgt tcatgccgca tctgacatga
    19381 acaaagcgca ggttgtcgcg attaatcgcg gaagtttttg aactggaacg gctgacccag
    19441 atcgccaccg cgcaccatcg ccatgacagc ctgcaaatca tcacgagatt tgccagtcac
    19501 gcggatctca tcgccctgaa tttgcgcctg cactttcagt ttgctgtctt tgatcatctt
    19561 gacgattttt ttctgggtcg cgctctcaat gccctgcttc agtttcgctt ccacaaacca
    19621 ggttttaccg ctatgaacga tattttccgg tacatccagt gaactgcctt caatgccgcg
    19681 cttcagcagc ttggcacgca gaatatccag caactgattg acctggaaat cggactcgct
    19741 caacactttg atggttttgc tggcgtcgtt cagctcaaat gaggcttcaa cgttacggaa
    19801 gtcaaaacgg gactccactt cgcggctcgc gttatcgacc gcgttacgtg cttcctgaag
    19861 atcaacttca gagacaatat cgaaagatgg catcttttcc tctcccttca tttttgatgc
    19921 gaagcataat acccgcaaag ttaaataacc gacagtcctg caccaggacg tatcccttaa
    19981 ttgtgcatag tcgcaacagc atgcacaatt aagggatagc cttattgaac acccggtgtg
    20041 gttgcgggtg aggaggaaca atgaaaatta ccgtattggg atgcggtgcc ttagggcaat
    20101 tatggcttac agcactttgc aaacagggtc atgaagttca gggctggctg cgcgtaccgc
    20161 aaccttattg tagtgtgaat ctggttgaga cagatggttc gatatttaac gaatcgctga
    20221 ccgccaacga tcccgatttt ctcgccacca gcgatctgct cctggtgacg ctgaaagcat
    20281 ggcaggtttc cgatgccgtc aaaagcctcg cgtccacact gcctgtaacc acgccaatac
    20341 tgttaattca caacggcatg ggcaccatcg aagagttgca aaatattcag cagccattac
    20401 tgatgggcac caccacccat gccgcccgcc gcgacggcaa tgtcattatt catgtggcaa
    20461 acggtatcac gcatattggt ccggcacggc aacaggatgg cgattacagt tatctggcgg
    20521 atattttaca aaccgtgttg cctgacgttg cctggcataa caatattcgc gccgagctgt
    20581 ggcgcaagct ggcaatcaac tgcgtgatta atccactgac cgccatctgg aattgcccga
    20641 acggtgaatt acgtcatcat ccgcaagaaa ttatgcaagt atgcgaagaa gtcgcggcag
    20701 tgatagaacg cgaagggcat catacttcag cggaagattt gcgtcattac gtgatgcagg
    20761 tgattgatgc cacagcggaa aatatctcgt cgatgttgca ggatatccgc gcgctgcgcc
    20821 acaccgaaat cgactatatc aatggttttc tcttacgccg cgcccgcgcg catgggattg
    20881 ccgtaccgga aaacacccgc ctgtttgaaa tggtaaaaag aaaggagagt gaatatgagc
    20941 gcatcggcac tggtttgcct cgcccctggt agtgaagaga ctgaagccgt caccacgatc
    21001 gacctcttgg ttcgcggcgg tatcaaagtc accactgcca gcgtagccag cgatggcaac
    21061 ctggcaatta cctgctcgcg cggcgtgaag ctgctggcgg atgcgccgct ggtcgaggtg
    21121 gctgatggcg aatatgacgt gatcgtactg cctggtggca ttaaaggcgc agagtgtttt
    21181 cgcgatagca ctctgctggt tgaaaccgtt aaacagttcc accgttccgg gcgtatcgtc
    21241 gcggctattt gcgccgcgcc agccaccgtg ctggtgccgc acgatatctt cccgattggt
    21301 aatatgaccg gcttcccgac gctgaaagac aaaattcccg ctgaacaatg gcaggacaag
    21361 cgcgtcgtct gggatgcacg ggtaaaattg ctgaccagcc aggggccggg gacagctatc
    21421 gactttggtc tgaaaattat cgacctgctg gttgggcgcg agaaagccca tgaagtggca
    21481 tcacaactgg tgatggcggc agggatttat aattattacg agtagtgtcg gatgcggtaa
    21541 gcgtcgcatc tgaccagatg cgacgttaca accacaaatt acgggcgata taccttcaca
    21601 ttgttaaagc cctgctcgcg cagatagagc gcctgcagac ggctcatcac cccgcgctca
    21661 caccacagta gccaggttct gttctggtcg agatcgccaa acttggtgct cagtttatag
    21721 aacggcagag aaactacatc aatcccttcg actttcagtg gcttatcttc ctgttcatcg
    21781 atagaacgga tatcgaggat cacgtcgttc gggccgaagc cattgacggt ttccacttcc
    21841 accacttcct gctcggtctg ctgggcgatt tcgcggatat caacgttatt cgcttcttca
    21901 accactttat cgagaatgct gaagtcgaat ttctcttctt ccgcttcaat cttcgactta
    21961 accgctttca ccgtcgggct tttggagata acaccgcaat attccggcat cgtgcgagca
    22021 aagtcttcgg tgccaatctg gcgagccagg ttgatgatgt gctctttgtc gtaagaaatc
    22081 agcggacgca ggatcagcgt atcggagacg ttatcaatca ggcgcaggtt ggtcagcgtc
    22141 tgactggaca cctggccgag cgcttcgccg gtgaccagcg cctgtacgcc gtaacgttca
    22201 gccactttag acgcggcacg caccatcata cgtttgagga taacgcccat ctgaccgtcg
    22261 tcgattttct cgagaatttc cccgacgacc ggttcgaaat taatagcgac aaaacgcacg
    22321 cggtgggagc tgccaaaacg gttccacaga taatgcgcca cctgacgcac gccaatttca
    22381 tgcgccgcgc cgccaaggtt aaagaaacag taatgcacgc ggcagccgcg acgcatcaac
    22441 atataactgg aaacaccgga gtcgaaacca ccggaaatga gcgacagcac atcttcctgg
    22501 gtgccgatcg ggaaaccgcc aataccttcg tagcggcctt taatcagcag gagacgatcg
    22561 tcttccactt ccagatggac agtcacttcc ggattggtca gcttcacgcg cgcggactca
    22621 atatgctgat ttaaaccgcc gccgacgtaa cgttccacat caatcgagct aaaatcatgt
    22681 ttgccacggc gcttcacgcg tacgcagaag gttttgcctt ccagctgatc gcgatactga
    22741 accaacgctt tctcaaaaat atcgtgcatg tcggtaaatg gcacgtcttc gacttcgaga
    22801 atatggtgga tacccggaat acgggtcagg gcgtcgcgaa tagccagacg ctggttttca
    22861 tcttttgcgc gaacttcgat gttatcccag tggcggacga cagcgagcgt ctcatcatag
    22921 tgctttaaaa cgttacgaat gttcccggta aggattttta taaagcgcaa gcgcacagat
    22981 tggcttttga tggtgatttc cgggaacaat ttaatgataa acttcatggc ggcaatggtt
    23041 cgttggcaag ccttaagcga cttgtatagg gaaaaataca gcagcccaca cctgcggctg
    23101 catccaggcg cggaagtata ccactaacat cgctttgctg cgcacatcac cttaccattg
    23161 cgcgttattt gctatttgcc ctgagtccgt taccatgacg gggcgaaaaa tattgagagt
    23221 cagacattca ttatgccgaa gaaaaatgag gctcccgcca gctttgaaaa ggcgctgagc
    23281 gagctggaac agattgtaac ccgtctggaa agtggcgacc tgccgctgga agaggcgctg
    23341 aacgagttcg aacgcggcgt gcagctggca cgtcaggggc aggccaaatt acaacaagcc
    23401 gaacagcgcg tacaaattct gctgtctgac aatgaagacg cctctctaac cccttttaca
    23461 ccggacaatg agtaatggac tttccgcagc aactcgaagc ctgcgttaag caggccaacc
    23521 aggcgctgag ccgttttatc gccccactgc cctttcagaa cactcccgtg gtcgaaacca
    23581 tgcagtatgg cgcattatta ggtggtaagc gtctgcgacc cttcctggtt tatgccaccg
    23641 gtcatatgtt tggcgttagc acaaacacgc tggacgcacc cgctgccgcc gtagagtgta
    23701 tccacgctta ctcattaatt catgatgatt taccggcgat ggatgatgac gatctgcgtc
    23761 gcggtttgcc gacctgccat gtgaagtttg gcgaagcaaa cgcgattctc gctggcgacg
    23821 ctttacaaac gctggcgttc tcgattttaa gcgatgctga tatgccggaa gtgtcggatc
    23881 gcgacagaat ttcgatgatt tctgaactgg cgagcgccag cggtattgcc ggaatgtgcg
    23941 gtggtcaggc attagattta gacgcggaag gcaaacacgt acctctgaac gcgcttgagc
    24001 gtattcatcg tcataaaacc ggcgcattga ttcgcgccgc cgttcgcctt ggtgcattaa
    24061 gcgccggaga taaagggcgt cgtgctctgc cagtactcga caagtacgca gagagcatcg
    24121 gccttgcctt ccaggttcag gatgacatcc tggatgtggt aggagatact gcaacgttgg
    24181 gaaaacgcca gggtgccgac cagcaacttg gtaaaagtac ctaccctgca cttctgggtc
    24241 ttgagcaagc ccggaagaaa gcccgggatc tgatcgacga tgcccgtcag tcgctgaaac
    24301 aactggctga acagtcactc gatacctcgg cactggaagc gctagcggac tacatcatcc
    24361 agcgtaataa ataaacaata agtattaata ggcccctgat gagttttgat attgccaaat
    24421 acccgaccct ggcactggtc gactccaccc aggagttacg actgttgccg aaagagagtt
    24481 taccgaaact ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc cgttccagcg
    24541 ggcacttcgc ctccgggctg ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca
    24601 acaccccgtt tgaccaattg atttgggatg tggggcatca ggcttatccg cataaaattt
    24661 tgaccggacg ccgcgacaaa atcggcacca tccgtcagaa aggcggcctg cacccgttcc
    24721 cgtggcgcgg cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca acctccatca
    24781 gtgccggaat tggtattgcg gttgctgccg agaaagaagg caaaaatcgc cgtaccgtct
    24841 gtgtaatcgg cgatggcgcg attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg
    24901 gcgatatccg tcctgatatg ctggtggtcc tcaacgacaa cgaaatgtcg atttccgaaa
    24961 atgtcggcgc gctcaacaac catctggcgc agctgctttc cggtaagctt tactcttcac
    25021 tgcgcgaagg cgggaaaaaa gttttctctg gcgtgccacc cattaaagag ctgctcaaac
    25081 gtaccgaaga acatattaaa ggcatggtag tccctggcac gttgtttgaa gagctgggct
    25141 ttaactacat cggcccggtt gacggtcacg atgtgctggg gcttatcacc acgctgaaga
    25201 acatgcgtga cctgaaaggc ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt
    25261 atgaaccggc agaaaaagac ccaatcactt tccacgccgt gcctaaattt gatccctcca
    25321 gcggttgttt gccgaaaagt agcggcggtt taccgagcta ttcaaaaatc tttggcgact
    25381 ggttgtgcga aacggcggcg aaagataaca agctgatggc gattactccg gcgatgcgtg
    25441 aaggttccgg catggtcgag ttttctcgta aattcccgga tcgctacttc gacgtggcaa
    25501 ttgccgagca acacgcagtg acctttgccg ccggtctggc gattggtggg tacaaaccca
    25561 ttgtcgcgat ctactccact ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg
    25621 caattcaaaa gcttccggtc ctgttcgcca tcgaccgcgc gggcattgtt ggtgctgacg
    25681 gtcaaaccca tcagggtgct tttgatctct cttacctgcg ctgcataccg gaaatggtca
    25741 ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc tatcactata
    25801 acgatggccc gtcagcggtg cgctacccgc gtggcaacgc ggtcggcgtg gaactgacgc
    25861 cgctggaaaa actaccaatt ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga
    25921 tccttaactt tggtacgctg atgccagaag cggcgaaagt cgccgaatcg ctgaacgcca
    25981 cgctggtcga tatgcgtttt gtgaaaccgc ttgatgatgc gttaattctg gaaatggccg
    26041 ccagtcatga agcactggtt accgtagaag aaaacgccat tatgggcggt gcaggcagcg
    26101 gagtgaacga agtgctaatg gcccatcgta aaccagtccc cgtgctgaac attggcctgc
    26161 ctgacttctt tattccgcaa ggaacgcagg aagaaatgcg cgccgaactc ggcctcgatg
    26221 ccgccggtat ggaagccaaa atcaaggcct ggctggcata atccctactc cactcctgct
    26281 atgcttaaga aattattcat ggcaggagtg gcagcatgca atacaacccc ttaggaaaaa
    26341 ccgaccttcg cgtttcccga ctttgcctcg gctgtatgac ctttggcgag ccagatcgtg
    26401 gtaatcacgc atggacactg ccggaagaga gcagccgtcc cataattaaa cgcgcactgg
    26461 aaggcggcat aaatttcttt gataccgcca atagctattc cgatggtagc agcgaagaga
    26521 tcgttggtcg cgcactgcgg gatttcgccc gtcgtgaaga cgtggtcgtt gccaccaaag
    26581 tgttccatcg cgttggtgat ttaccggaag gattatcccg tgcacaaatt ttgcgctcta
    26641 tcgacgacag cctgcgacgt ctcggcatgg attatgtcga tatcctgcaa attcatcgct
    26701 gggattacaa cacgccgatc gaagagacgc tggaagccct gaacgacgtg gtaaaagccg
    26761 ggaaagcgcg ttatatcggc gcgtcatcaa tgcacgcttc gcagtttgct caggcactgg
    26821 aactccaaaa acagcacggc tgggcgcagt ttgtcagtat gcaggatcac tacaatctga
    26881 tttatcgcga agaagagcgc gagatgctgc cactgtgtta tcaggagggc gtggcggtga
    26941 ttccggcgcg ggggcgactg acgcgtccgt ggggagaaac taccgcacga ctggtgtctg
    27001 atgaggtggg gaaaaatctc tataaagaaa gcgatgaaaa tgacgcgcag atcgcagagc
    27061 ggttaacggg cgtcagtgaa gaactcggtg caacacgagc acaagttgcg ctggcttggt
    27121 tgttgagtaa accgggcatt gccgcaccga ttatcggtac atcgcgggaa gaacagcttg
    27181 atgagctatt gaacgcggtg gatatcactt tgaagccgga acagattgcc gaactggaaa
    27241 cgccgtataa accgcatccg gtagtaggat ttaaataaga gtccaggcct gatgagacgc
    27301 aacaagcgtc acatcaggca tcggtgcaca actacgacag aatgcccagc ggccagtgat
    27361 gaccaataaa gtacaggatg ccagcggaga tcaccccggc gacaatatca tctatcatga
    27421 tccccatgcc gccatgcaca ttgcgatcaa accagcggat cggccacggt ttccacatat
    27481 ccagaatacg gaaaatcaca aacccggcgg caacccactg ccagtcattg gtcggcagcg
    27541 ccatgagcgt gatccacata ccaataaatt cgtcccagac aatgctgccg tgatcgtgca
    27601 cgcccatatc ttttgcggtc tgatgacaaa gatagacgcc gatacagatc cccagcatca
    27661 ccaccagcga gtagagctgc cagggcaaaa aggtcatcag ataccagaac ggaatcgctg
    27721 ccagcgagcc catcgtccca ggaacgatcg ggcttaatcc acttccgaat ccgacagcaa
    27781 gtagatgcca cggattactc atcttcaggc gacttttcgc gacatcttta tggcgtggca
    27841 aaatggtcat atcctttcca gtctaacgta acaggttcac cgtcacgaat aaaacaaagc
    27901 ccttcgatat cggcggtcat ttgcccgata caggtaaacg gtacgcccag gtgtccgaga
    27961 gccacatcca gcgctccacg gttcagttcc ggcaccgtga aacacaactc gtaatcttca
    28021 ccgccagaga gcgcccagcg cagcgcctgt tccggttcaa catggcgaga aagcgcatca
    28081 gaaaacggca gcaatgccag gtcaatacgt gcgccgcagt cgctggcttt cacgatatgc
    28141 ccgagatcgg aaatcagacc gtcagagaga tcgatagctg aatttgccag atcgcgcagt
    28201 gcctgcccct gtaaaatacg cggcgatgga cggagatgac gtttgatcaa gtagtccgca
    28261 tctttagcat cggcaacctg caaacggttt tgcaaaatcg ccagcccggc ggcgctatcg
    28321 cccggtgtac cggtcacata gatccagtca cccggtttcg ccccaaagcg cgttaaggct
    28381 cgtcccatcg gaacaaagcc gtggataccc aacgtcattg ataatggccc acgcgtggta
    28441 tcgccgccaa tgagttgcat atcgtaataa ttgagaagat caaacaaact gtcgctgaag
    28501 gactcaagcc acgcttcgtc tacgtccggc aaggttaatg ccagcgtcag ccaggccgga
    28561 tcggccccca tcgctgccag atcgcttagg ttcaccgcca gtgctttata cgccagatca
    28621 gcaggatcga tatcagggag gaagtggttg cccgccacca gcgtatcagt gctgatcgcc
    28681 agggtctgtt tctcggggat attgagaagt gcgcaatcgt cgccgatgcc cagttcgaca
    28741 tcaagacgag aacttcttac acggtcaaaa taacgggcaa tcagggagaa ctcgccacat
    28801 gccatacgtt atgcctcagc aggtaaaaaa gaaaaggccg gcgatcatgg aacggtcttc
    28861 cgtgaatcta ccggcctgga tattactttt tgttagggcg aatcacaggt gctgctttat
    28921 cgagtacacc gttgacgaac ttatggctgt cttctgcgcc gaacgatttc gccagttcga
    28981 tcgcttcgtt aatggccact ttgtatggca catcgctacg tttagacagt tcgtacagcg
    29041 caatgcgcag tactgctttt tctacctgtc ccagttcttc cagcagacgg gacaggtatg
    29101 gcttcatcag tccgtcgagg tatgcggtat tagtcgccac cccggccagc agctcacgga
    29161 agtacaggac gtcaacgtct tttacatcct gttcagccag gaactggtat tcaacatcag
    29221 cgatgtcgtt ctgggacaac tgccaggagt agagcgcctg gacggcacac tcacgagcgc
    29281 ggcgacgagc agcaggtttc acggatttcc ccttactaat ttcaggcctt gatggctttc
    29341 aatacattaa tcatttcaag cgcggtcagt gcagcttctg cacctttgtt gccagctttg
    29401 gtgccagcac gttcgatcgc ttgttcaatg ctttcagtgg tcagaacccc aaaagcaacc
    29461 ggaatttcgc tgtcctgggc aacatgcgcc aggccgttgc ttgcaccacc agcgacatat
    29521 tcaaaatggg cagtgccacc acgaataacc gtacccagcg caatcaccgc gtcgtattta
    29581 ccggttttag ccagtgcacc cgccgccagc ggcagctcat aggcaccagg cacccaaaca
    29641 acggtaatgt tttcatcttt tacctgaccg atacgtttca gtgcgtcaat tgcaccttcc
    29701 agcaggctgt cattgataaa gttgttgaaa cgcgcgatgg tgatggcgac gcgagcgtcc
    29761 ggggtagcaa cgttagcttc aataatgttc attttcttcc ttcgggttcg agtatggccc
    29821 cgcagggggg cggattttag cataatattt cgtgcgctgc ttccctttcg agccgggaga
    29881 tcatgcaccc actaaatgca ggcaaacatc cgggcctaca tgacgtatct ctttgaattt
    29941 aaattggggg gcgtcggcta atttctcaag ccctggcagg gtgcataatc cacgggcgtc
    30001 gctgcctaat agtttaggtg cgatatagac aatcagctca tcgactaaac ccgcctgcag
    30061 caatgcgcca gcgagcgttg gccccgcatc cacccagatg ctgttaatct gctgtttacc
    30121 cagttgcatc atcagtacaa ccagatccag atgaccttta tgctctggaa tcagcaaggt
    30181 acgcaccgtt tccggccact cacgagaatc ttcctgcgta cgcgcgaacc aggtttcgcc
    30241 gggctgctgc acaatgtgat gttccggcgt cacgcgattt tggctatcaa tcacaatacg
    30301 taccggctgg cggagatttt gttgcgggta gagcgcctga gtttgttcat ccagttcgga
    30361 ccaacgcacc gttaaggcag gatcatccgc cagcaccgtg gcgctgctgg ttaaaatggc
    30421 atgactttgc gcgcgcagtc gttgtacatc acgccgcgcc tggggcgaag tgatccactg
    30481 gctttcgccg ctcgccatcg ccgtgcgacc atcaagcgat gcgccaagtt ttaactgaat
    30541 ataaggaaag ccggtgcgca tccgcttgag aaagccttta ttcaattgct cggcttcact
    30601 catcatcagg ccgtggctga cgtcaatgcc agcctgttgc agacggtaaa gtccacgccc
    30661 agcgacctgc gggttaggat cttgcatcga ggcaaccacg cgcgccacac ctgcggcaat
    30721 taaagcatca cagcaaggcg gcgtacggcc gtgatggctg caaggttcga gtgtcacata
    30781 cgccgttgca cctttggctt tttcacccgc catacgcaac gcgtgtactt cggcatgtgg
    30841 ttcacccgct cgttgatggt aaccttcacc gacaatttcg ccatctttga caatgacgca
    30901 cccgacattt gggttggggt gggtggtaaa acgtcctcgt tgcgccagct ttagcgcccg
    30961 cgccatgtaa tactcgtcct gcacggctta gtcctccagg cgcgcgatct cttcgccaaa
    31021 ttctttgata tcttcgaaac tgcggtagac agaggcaaaa cggatatagg cgactttatc
    31081 gagctttttc aattgctcca tcaccagatt gccaatcatc ttgctcggta cttcgcgctc
    31141 accggtggcg cgcagctgcg atttaatatg attgattgcc atttcaacgt catcggaact
    31201 caccggacgt ttttccagcg cccgcagcat tccgctacgc aatttctctt cattaaacgg
    31261 ttcacgcacg tcgttgcttt ttacaacacg cggcataacc agctccgcca cttcaaaggt
    31321 ggtgaaacgt tcattacaca ccagacactg ccgacggcgg cgtacggatg aaccctcgcc
    31381 cacgagacga gagtcaatta ccttagtgtc cacggcgaaa cagaatgggc aatgcatacg
    31441 gcgtcctgac caggtggtta acggaaatct attttaccct gaactgacga tacaacaaag
    31501 gcgcaacgct tttgagataa aggatcgatg ctttcgctgc ttttaccctc taccattagg
    31561 ctaattgcga cgagaaaggg acgacctatg aatactaacg tttttcgact gctcctgctg
    31621 ggaagcctgt tcagccttag cgcctgtgtg cagcaaagtg aagtgcgaca gatgaaacac
    31681 agcgtcagca cgctgaacca ggagatgacg caactcaatc aagaaacagt caaaatcact
    31741 cagcaaaaca ggctgaatgc aaaatccagc agtgggggtt accttctgcc tggagcgaaa
    31801 acaccggcaa gactggaaag ccagatcggt actttacgta tgtcgctggt gaatattacg
    31861 cctgatacag atggcaccac ccttacacta cgtattcagg gtgagtcgaa tgatcccttg
    31921 cccgcattca gcggaactgt tgaatatggg caaattcagg gaacaataga caactttcag
    31981 gaaatcaatg tgcagaatca attgattaat gcccctgcca gcgtcctcgc ccccagcgat
    32041 gttgatattc cgttacaatt aaaggggatt tctgtggatc agttaggctt tgtacgtatc
    32101 cacgacattc aacctgttat gcagtaaacg tatttcggga cgattttgtg cgtcccgcaa
    32161 catctttccc cgtcattttg ttactctgct tacatcacct agattgatag taaaagtttg
    32221 caacaagggc gaaagtcagt acaatccccg ccctaatgtg tgtaaacgtg aacgcaatcg
    32281 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    32341 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    32401 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgtggtact
    32461 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    32521 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    32581 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    32641 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    32701 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    32761 caatactgat cttaacttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    32821 cgacatgggt cgtaataaaa atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    32881 catcgatact ggtctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    32941 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    33001 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    33061 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    33121 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    33181 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    33241 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tcgtaggtta
    33301 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    33361 gagctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    33421 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    33481 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    33541 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    33601 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga caaagcaatt cccagttact
    33661 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    33721 gttggaataa acaaactcgc gggaacagcg accgcagacc cacggatata tttttaatgc
    33781 tttttcgcga tagccgcttt ctaaccgcgc atagtttttt gggatgatag ccatgaagag
    33841 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    33901 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    33961 gattacggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    34021 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ccacatagat ggaagatgca
    34081 gtaccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    34141 aagaggtaca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    34201 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    34261 aagttttcac gaatacggtc cgataccacg atactgtcgt taagcgagta accgataacc
    34321 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    34381 agcgtaatga taacgtcgtg cgccagcgca ataacaaccc ctgccgccag tcgccactca
    34441 aagcgaaaac ctacgtacac aaggatagac agcagcgccg ccatcaacgc catcgcaccg
    34501 gtttgcgcaa ggtctgcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    34561 ttctgattgg tggattcgtt aatcaccttc agaacctggc tgcccagcac ctgaccgccg
    34621 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    34681 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    34741 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    34801 ccgcgcacgc ccataataac gatagcggcg attaacagca gaccagagat gccgaaagcc
    34861 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    34921 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    34981 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    35041 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    35101 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    35161 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    35221 gatcagtacg ttcgcatcga ccgccaccgc aagggttaag acgatacccg cgatacctgg
    35281 catactcagc gttgcgcctg gcaacagcga cataatgccg acgattaaga tcaagttggc
    35341 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    35401 agaaaccagc agaccggcaa ggcaagcctc cagcccctgt tcaatgttct gcatacccag
    35461 ggttgggcca atggtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    35521 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    35581 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    35641 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    35701 cggtttgccg atgttgtcct tagtgaagtt agacatgatg ttaccaccag cgctatcgag
    35761 cgagatgtta acctgcggtt ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    35821 gtcaccggtc agaattacgc gtttgtacag cacaactgac tgaccttcgc gggtctgttt
    35881 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctggtcaa cgttggtgtt
    35941 taccagacgg aattccagcg ttgccgtcgc acccagaatc tctttcgcac gcgcagtgtc
    36001 ctgaatacct ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    36061 cgctacgcca agttggttta cacggttacg caggatattg atgttctgct gcaccgcata
    36121 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    36181 gctactaatc actaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    36241 tttagcatcg cggaaagtaa tgctcaggcc gtagttgttt tctttacgaa cggtggtata
    36301 cgggatgccc ttttcacgca gatcgctgcg caggctatcg atattttgtt cctggagttt
    36361 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    36421 gccgagcttc atcggctcag cgtgaatagc tgccagccag cgcggcgttg ccggggcaag
    36481 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcag
    36541 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    36601 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    36661 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    36721 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    36781 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    36841 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    36901 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    36961 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    37021 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    37081 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    37141 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    37201 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatgg
    37261 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    37321 gcgcgcctaa tatttcgttg caacggtcaa gatgatgcaa gtaagcgcgt gaataattgc
    37381 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tacttcgcat
    37441 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    37501 gcattacgca gtcaaacata tcgataccac gacgtacgcc ttcaaccagg tcttctggtt
    37561 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcgggcat acatgctcca
    37621 gaatgcggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    37681 caaaaccgat atctaccaga cctttaacag aaatatcacg taaatcttcg taaacgctgc
    37741 cctgaatgat accaaacagc gcatttttgt ttccgagact gtcaaaacgc tcacggctac
    37801 gcttcgccca acgcagagac atatccatgg agcgttttgc gtaatcccag tcagcaggat
    37861 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    37921 ttgatttttc aggatcgagg aaaatcggat cgccgttgat cgggttacgg aagtgcacgc
    37981 cctgttcggt gattttacga atatcgccaa ggctgaagac ctggaagccg ccggagtcgg
    38041 tgaggatcgg ccccttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    38101 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    38161 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    38221 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    38281 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgtttt
    38341 aaaccagcgc cgcggaatta ctccccgacg cgctcattaa ttgcctgcgg attgtacgta
    38401 ataaacatcg catcaccgta actaaaaaag cgatatttct cttctaccgc tgctttatag
    38461 gcgttcatgg tgtgttgata accggcaaag gccgaaacca gcataatcag cgtcgactct
    38521 ggcaagtgga agttcgtcac cagcgcatcg accactttgt actggaagcc cggatagata
    38581 aagatttggg tatcgtcgaa gaacggttca atgagatcgt ttttcgctgc ctgagccgcg
    38641 ctttccagcg aacgtactga agtggtgcca accgcaatca cccggttacc gcgcgctttc
    38701 gccgccagta ccgcgtctac cacatcctgc ggtacttcag cgtattccga gtgcatgatg
    38761 tgatcttcaa tggtgtcgac gcgcaccggc tggaaggtgc ccgcaccaac gtgcaacgtc
    38821 acaaacgcca tctccacgcc tttggcgcgc aatttttcca gcaaaggctc gtcaaaatgc
    38881 agaccagcgg tcggggctgc aaccgcgccc ggtttttcgc tataaacggt ttgataaagt
    38941 tcgcggtcag cgtcttcgtc cggacggtcg atatacggcg gcagcggcat atggccgata
    39001 ctgttgagga tatccagtac cgaacgatca tcattgaatt cgacttcaaa cagtgcgccg
    39061 tggcgcgcgg tcattgttgc gttaatactt tcgtcatcgc ccagcagcag ttctgcgcca
    39121 ggttttggcg ctttcgaggc gcgaatatgc gcaagaatgc gtttgtcgtc gagcatccgc
    39181 tcaaccagca cttcaatctt gccgccgctg gctttacgcc caaacaggcg cgccgggatc
    39241 acgcgagtat tattaaaaac cagaagatcg ccggggttga gcttatcgag taaatcggtg
    39301 aaagtaccgt gcgtcagcgc gcccgtaggc ccgtccagcg acagtaaacg acagctactg
    39361 cgttcaggca tggggtagtg ggcaatcagg gattcgggca attcaaagga gaaatcggta
    39421 acgcgcatga cactgactca gactaaaata agaggcgggt agtctagtgc cggggcgctc
    39481 tccctgcaac ctttaccccc ctttggatag aatataatcg atgaattttt tagctcacct
    39541 gcatttagcc catctcgcgg aaagctcgct ttccggcaat ttactggctg atttcgtacg
    39601 cggaaatcct gaagaaagtt ttccgcccga cgtcgtggct ggcattcata tgcatcgacg
    39661 tatcgacgta ttgactgaca atctgccgga agtccgcgaa gcacgggagt ggtttcgtcg
    39721 tgaaacgcgc cgcgttgcgc ctattacgct ggatgtcatg tgggatcact ttctttcccg
    39781 ccactggtcg cagctgtcgc cggactttcc gctacaggaa tttatctgtt atgcccgcaa
    39841 gcaagtgatg acgattttgc cggactcacc gccacgtttt atcaatctaa acaattactt
    39901 gtggtcagag cagtggctgg tgcgctatcg cgatatggat ttcatccaga acgtgttaaa
    39961 cggcatggca agccgtcgcc cacgtctgga tgccctgcgt gactcctggt acgatttaaa
    40021 cgcccattat accgccctcg aaacccgctt ctggcagttt tatccgcgga tgatggcgca
    40081 ggcgtcacac aaggcgttat aaaacgttag ttcatccata ccgtagccga aatggcaggc
    40141 aaagccagaa tcccgtcagt cagttgccca tgcccttctt tgcattgcca ttgcacggca
    40201 ttgagaaacg gtgacgcggg tagcaccact tcacaggcct cgccacggtt gattgccacc
    40261 agtacacgtt gctgattcag cacgcggaca aataccacta cgttatcttc cgcatacagc
    40321 acctgacagc cgccacgacg cagcgcctga cttttcttac gcagcacaat cattcgccgg
    40381 tacagcgcga ataacgccgt atcctgcttt tccacctgcc aggggaacgg tttacggcaa
    40441 aacggatcgt ttttaccatc cagtcccact tcatcaccgt aataaatgca cggtacacca
    40501 ggccaggtga acagccagac caccgccagc ggcaggcgcg caatatcccg accgagcagc
    40561 gttttaaatc gcgcagtatc gtggctgtcg agctgattaa acatacgtaa ttgttgttga
    40621 tgagaaagcc ctgcgcggta attatccatc caggccatac aggtttgggc atcaatttgc
    40681 tgcggatcgt aagagatatc ggtattggca agaaatcccc acaacgggaa tgtgaagcca
    40741 cgatagttca tggcggcatc ttccacatcg gcctgtaacc attgccgtgc atcgccaaaa
    40801 tgttcgccga caatatacgc ttccggctgg gtttctttcg ccgcttcggt gatcccagca
    40861 acgtgctgca tattattgcg cgccccaccc gcctccccca gcatatgcac cacatccagc
    40921 cgccagccgt ccatactcca cggcgctttc agccaatggc ggacaatact gtcttccccg
    40981 cgataaattt cattcaccag actttccgac tgataatcca gcttcggcaa gctggcatag
    41041 ccaagccagt cgagcgccgt gccatcatca ctaaacgagt accagtcgcg ccagggcgat
    41101 tcagggttgt gacaagctcc ccccgtgcca cgattgtgcc tgtcaaacca ggcatgggaa
    41161 tcgccactgt ggttaaacac gccgtccagc accagccgca ttcccagctg ctgcgtattg
    41221 tgtcgcaaaa gcagcaacgc cccatcaccg ccaaactgcg gatcgacatg gcgataatcc
    41281 tcggtatcgt attgatgtac gctgggagct ttaaacaccg gattgagata cagcgctgtc
    41341 acgccaagct ttttcagata cggcagtttt tcgcttatcc cgtccagatc gccgccatag
    41401 aacgttgatc cgcccgcctg cgccgtgacc ggttcatccc agtcacgcaa gatgatctct
    41461 tgtccggctg catgatggta atagacatga tcctgttcag cttcacgagg aagactacgc
    41521 gcaaaacgat cagggaagat ctgataaaaa atctgatccg cagcccattg tgggccgata
    41581 tccggtacat cgacggcaaa ctgctccagt cgtgccggcg gcattcggct gaagccctgc
    41641 ggtgacgcct ggctgcggct gactgcgctg cttatgcatt ggtactgaca tttcttcgtt
    41701 atcgtgttct gtacgcagca taatgcgctg cggtgggtct tcgcccgtca gccacagtgt
    41761 aatgagcagt tgatctttgc tttgtttaac aaatgggggc accggcaggt gccatgcatt
    41821 taacatcata attcccctgt gatgaaaatc tgatcacctt gccatagcct aatgcaggat
    41881 cactcatcat ctgaaggata tttgggggag gaggacgggg gcggataact ttcggaatag
    41941 ccggatcagg atttcctcct gaaccggctt acagaacgca aggattactg gttttcagcc
    42001 agctgacgat cgtggcgacg cttaaacatc cagccaatca acagcacaac aatccacgcg
    42061 aaaccgacat acagcgagat acgcgtatcc gggtgataac caatcaaccc gataataaag
    42121 agcaggaaaa tcagcccgcc gatggtcgtt gctaccccac ccggcacttt aaatttcagc
    42181 gccttaactt cttctggcgg caaacggcga cggaaggcga tttgcgacag caggatcata
    42241 atccacaccc ataccgtggc gaaggttgcc agcgaagcga tcaccaggaa gacgttttcc
    42301 ggcatgatgt agttcagata caccgcaaac agcagcgcgg tagtcatcac cagcaccgta
    42361 acccacggaa taccgcgacg cgacgttttg ctgaaaattt tcggtgcgct gccctgctct
    42421 gccataccgt ggagcatacg gcctacgcca aatacgtcac tgttaattgc cgacagcgaa
    42481 gcggtcagca caacaaagtt aagaatgctg gcggcaaagg taatgcccat atgctggaac
    42541 gtcagcacga acgggctacc ggcagtgcca acctgattcc acgggtagat agacataatg
    42601 acgaacagcg taccgacgta gaacaccaga atacgcatcg gcacggagtt aatcgcacgc
    42661 ggaatcgatt tctcaggatc tttcgcttca ccggcggtaa tcccgataat ttcgatccca
    42721 ccgtaagcaa acatcaccat ttgcaacgac attaccatgc caagccagcc gttactgaag
    42781 aagccgccgt tgctccacag gttatgaata ccggtcggtt gcccgccgtt gccaatcccc
    42841 cagatgatga tgccgaaacc ggcgacaatc atgatgatga tggtggcgac tttaaagaac
    42901 gagaaccaga attccagctc accgaatacc ttcacgctca tcaggtttac ggcgcaaatg
    42961 atcagcacca cgctcagtac ccaaatccag tgcggcaccg tcgggaacca gacacccata
    43021 tagataccaa aagcggtcac atcggcgatg gcgacaatta ggatttcaaa gcagtaggtc
    43081 cagccggtaa tgtaacctgc cagcgggccg aggttttcct gcgcataacg cgagaaagag
    43141 ctggcggccg ggttatgtac cgacatttcc cccagcgcac gcataatgat atacgccgcg
    43201 ataccaccga taatataggc caacaacacg ctcggaccgg ccattttgat ggcgtctgcc
    43261 gaaccgtaaa acagcccggt gccaattgct gaacccagtg ccataaagcg tatgtggcgg
    43321 gtacttagcc cacgctttag cttgttctta ctttccatca attcctaacc catcaacaca
    43381 ataaaaaacc acggggcatc gcagccccgt ggttaaaaca aatgttcagt gatttagtga
    43441 gcgctggagg tcacctgacg acctgccgcg cgatcccaga taatggccag aaccaccatc
    43501 accactgttg gcattaacca cgccaggcct tgttcggcca gcggtaaacg ctgcgcccag
    43561 gacggtaaga tatcgctgaa tgcagatgct ttgatcccgt cgagaatacc aaaaagcagg
    43621 ctgataaaca tcggcggggc aatcacgcgg gacgaattat gccaccatga gcgtgtaaaa
    43681 cttaatacaa ccagtgcgat acacggcgga taaatagcgg tcagcaccgg tacggagatc
    43741 tggatcagct ggcttaagcc gaggttagac accaccatcg agaagccgcc gaggataaac
    43801 accagcgtac gataagagag cggtacgtac tgggcaaaga attctgcaca agcacaggtc
    43861 aggccaactg ccgttaccag gcaggcgatg aagattaacg ccgccagcag gaagctaccg
    43921 ccgccgccaa atgtgtgctg aacgtaagca tgcagaatag cagcgccgtt tgcagactga
    43981 tcgaccagcg acgcgctgtc tgaacccaga cggaacagcg ccaggtacag cagagtcaga
    44041 ccaacacccg ccatcaggcc agcccagacg gtataacggg tcagcagacg cgcttcggta
    44101 acgccacgag aacgcgccgc gttaacaata acgataccaa acaccattgc gcccagcgta
    44161 tccatggtca gatagccgtt aacgaagccg ttagaaaacg cagcgttttg ataagcctca
    44221 gtcgccgtgc tgatagaacc cgccggccag acaatagcgg caacagacag gatgaccagc
    44281 gcgataattt tcagcggcgc aaggaagttg cccacggtat ccagcagctt gcccggatag
    44341 agcgaaacca gaataacgat agcgaaatag accaggctgt agataaacag cggcagcgcg
    44401 gaatcacccg tcagcggcgt aatccccact tcaaaggaaa cggtagctgt acgcggcgta
    44461 gcgaaaagcg gccccaccgc caagtaacaa accgttgcca gcagcacgcc agcgacttta
    44521 ccgattggcg tgctgaggct gtcaacaccg ccgccaactt ttgccagcgc cactaccgtt
    44581 aataccggca ggccaacggc agtaatgagg aagccgaatg ccgcagtcca gacgtgttcg
    44641 cctgcctgca agccgaccat tggagggaaa ataatgttac ctgcgccgac gaacaacgca
    44701 aatgtcataa agcccagagc gatgatatcg cgcgatctta attgatgggt cataaatcat
    44761 actgcctgtg gatgtggtgt tgtaacgtta aatttttgtg ccaatccctg gggatgatac
    44821 agcggtatat ttaaacaatt ttagcgggaa atactcccaa ccaggcgcgg cgaagaatag
    44881 tttttcgatt taccgcgtta atgcagtcag tatgacagca cctgaagcgc aatttaaacg
    44941 cttataacgt tttaaagcaa ggagggatct aaaaaccaga ggtatatagc gataagaaat
    45001 caccaaccag tataaaacac tatttccgtg aaaaagacat ggctaatcac gcgaacaaaa
    45061 aatcaccaac cgttgtaaaa tctaacgccc gtattttgac tgcaaagaaa ataagccagc
    45121 atcgactggc ttatggcaat aaaagatgac aaaggcggat taatcgctgt ttttggcaat
    45181 taaacgttcc gggataacaa aactgaaacg tgttcctttt cctactgtac tctcaatatt
    45241 caggcgactt tcgtgatgat tcacagcatg tttcacgatc gctaacccta atccgctacc
    45301 gccggtttgc cgggaacgcg ctttatcaac gcgataaaaa cgctcggtca ggcgcggaat
    45361 atgctccggt gcaatgcccg gtccgttatc ttcaacgcta aattcggcac cgtgcggcac
    45421 tcgctgccag cgtacggtga tatgcgtgcc ttctggagta tgattcacgg cgttatagac
    45481 caggttcgaa atcgcactgc gtagctgatc ttcgttgcca gacactttga ggccgttatc
    45541 tatctcaaag gtaaatgtct gttttttctg actcagagtc tgagcctcgc gctcaacaac
    45601 gcgcagcatc atcggcacat caaccttttc attgagcaat tgcgtcggcg cggcttcaat
    45661 tttcgacagc gttagcaact gcttcaccag cccttccatc cgctgggtct gctcgcgcat
    45721 ggtgtgcaac gctttttcgc gtaccgcgcc ttccagcggc tgctcatcca tcatctccag
    45781 gtaaccctgt aacacggtca atggcgtacg taactcatgg ctcacgttgg caaaaaagtt
    45841 acgccgcgcc ccttccagtt gatgcatttg cgtgacatca cgcgccacca ttagcaactg
    45901 tttgtgggta taaggcatga cgcgaatttc cagatgccgc ccggtgttga gcaccagatt
    45961 gagcgggcga gaaaaatcac gcgttttcag atattgcgta aactccgggt aacgcagtag
    46021 gttaaggatg ttctgcccgt tatcttccgg ccagcgcaaa ccaagaattt gttgcgctag
    46081 accgttacac cagaaaatac cgccctcttc cgtggtcagc accaccgcgt cgggcagcga
    46141 ctccgcgccg ctacgaaagc gtttaatcag attgcccagt tcacggcggc gttttttatt
    46201 tcgcagctgc atctggtgta agccgtatag tagcggttcc cagctaccac gccccggtgg
    46261 cggggtcata atgcgatcca cccacaacca ccatgaaagg cgcaataaat tccagaaatg
    46321 ccagataagc agtcctgtta ccgatgccag caaaaaccag ggcaggtaac caaaaaatgc
    46381 acccaggatg aaagccggga ggcagcaaag tagcagctcc agcaccagcc ttttccacga
    46441 cagccgttcc agcaggtaag atactccagt taagaaatca taagccctgc tctgcgtccg
    46501 atgagcaagg cgttaaaagc gggttgaaaa acgatatcct gtaccgcgca cggtctgcac
    46561 catgcggtca tgcccgccgg gctccagtgc tttacgcagg cgacgaatgt ggacatcgac
    46621 cgtgcggtct tccacataca cgttagttcc ccagacgtgg tttaacagct gctcgcggct
    46681 gtacacgcgc tcaggatgcg tcataaaaaa gtgcagcagt ttaaattctg tcggccccat
    46741 ctccagcggc tcttcgcccg ccatcactcg gtgagatgtc gggtcgagac ttaatccctg
    46801 catctcaatc acctcttcca ccgccattgg cgaaatacgg cgcattaccg ctttgattcg
    46861 cgccaccagc tccttcggcg aaaacggctt ggtgatatag tcatccgcgc cggtttcaag
    46921 gccgcgcacg cgatcttctt cttcccctct ggcggtcaac atcaccactg gaatatcccg
    46981 ggtcatcgac tcgcgcttga ggtgtttgat gaactggata ccggagccgc caggtaacat
    47041 ccagtcaagg agaattaaat ccggccaggg ttcattcagt tgattcacag cactgtcata
    47101 atcttccgct tcgaccggct gaaagccatt ttgttcgagc acgaagcaga ccatttcgcg
    47161 aattggagct tcatcttcta cgaccagaat acgtctcgcc atgatttgcc ctgttgtaat
    47221 aaataggttg cgatcattaa tgcgacgtca ttatgcgtca gatttatgac agatttatga
    47281 aaagctcgtc gcacatatct tcaggttatt gatttccgtg gcgcagaaaa aagcaaatgg
    47341 cacatctgtt tgggtataat cgcgaccatg ctttttcgcc agggaaccgt tatgcgcatc
    47401 cttcacacct cagactggca tctcggccag aacttctaca gtaaaagccg cgaagctgaa
    47461 catcaggctt ttcttgactg gctgctggag acagcacaaa cccatcaggt ggatgcgatt
    47521 attgttgctg gtgatgtttt cgataccggc tcgccgccca gttacgcccg cacgttatac
    47581 aaccgttttg ttgtcaattt acagcaaact ggctgtcatc tggtggtact ggcaggaaac
    47641 catgactcgg tcgccacgct gaatgaatcg cgcgatatca tggcgttcct caatacaacc
    47701 gtggtcgcca gcgccggaca tgcgccgcaa atcttgcctc gtcgcgacgg gacgccaggc
    47761 gcagtgctgt gccccattcc gtttttacgc ccgcgtgaca ttatttccag ccaggcgggg
    47821 cttaacggta ttgaaaaaca gcagcattta ctggcagcga ttaccgatta ttaccaacaa
    47881 cactatgccg atgcctgcaa actgcgcggc gatcagcctc tgcccatcat cgccacggga
    47941 catttaacga ccgtgggtgc cagtaaaagt gacgccgtgc gtgacattta tattggcacg
    48001 ctggacgcgt ttccggcaca aaactttcca ccagccgact acatcgcgct cgggcatatt
    48061 caccgcgcac agattattgg cggcatggaa catgttcgct attgcggttc ccccattcca
    48121 ctgagttttg atgaatgcgg taagagtaaa tatgtccatc tggtgacatt ttcaaacggc
    48181 aaattagaga gcgtggaaaa cctgaacgta ccggtaactc aacccatggc ggtgctgaaa
    48241 ggcgatctgg cgtcgattac cgcacagctg gaacagtggc gcgatgtatc gcaggagcca
    48301 cctgtctggc tggatatcga aatcactact gatgagtatc tgcatgatat tcagcgcaaa
    48361 atccaggcat taaccgaatc attgcctgtc gaagtattgc tggtacgtcg gagtcgtgaa
    48421 cagcgcgagc gtgtgttagc cagccaacag cgtgaaaccc tcagcgaact cagcgtcgaa
    48481 gaggtgttca atcgccgtct ggcactggaa gaactggatg aatcgcagca gcaacgtctg
    48541 cagcatcttt tcaccacgac gttgcatacc ctcgccggag aacacgaagc atgaaaattc
    48601 tcagcctgcg cctgaaaaac ctgaactcat taaaaggcga atggaagatt gatttcaccc
    48661 gcgagccgtt cgccagcaac gggctgtttg ccattaccgg cccaaccggt gcggggaaaa
    48721 ccaccctgct ggacgccatt tgtctggcgc tgtatcacga aactccgcgt ctctctaacg
    48781 tttcacaatc gcaaaatgat ctcatgaccc gcgataccgc cgaatgtctg gcggaggtgg
    48841 agtttgaagt gaaaggtgaa gcgtaccgtg cattctggag ccagaatcgg gcgcgtaatc
    48901 aacccgacgg taatttgcag gtgccacgcg tagagctggc gcgctgcgcc gacggcaaaa
    48961 ttctcgccga caaagtgaaa gataagctgg aactgacagc gacgttaacc gggctggatt
    49021 acgggcgctt cacccgttcg atgctgcttt cgcaggggca atttgctgcc ttcctgaatg
    49081 ccaaacccaa agaacgcgcg gaattgctcg aggagttaac cggcactgaa atctacgggc
    49141 aaatctcggc gatggttttt gagcagcaca aatcggcccg cacagagctg gagaagctgc
    49201 aagcgcaggc cagcggcgtc gcgttgctca cgccggaaca agtgcaatcg ctgacagcga
    49261 gtttgcaggt acttactgac gaagaaaaac agttacttac cgcgcagcag caagaacaac
    49321 aatcactaaa ctggttaacg cgtctggacg aattgcagca agaagccagc cgccgtcagc
    49381 aggccttgca acaggcgtta gccgaagaag aaaaagcgca acctcaactg gcggcgctca
    49441 gtctggcaca accggcacga aatcttcgtc cgcactggga acgcatcgca gaacacagcg
    49501 cggcgctggc gcatactcgc cagcagattg aagaagtaaa tactcgctta cagagcacaa
    49561 tggcgcttcg cgcgagcatt cgccaccacg cggcgaagca gtcagcagaa ttacagcagc
    49621 agcaacaaag cctgaatacc tggttacagg aacacgaccg cttccgtcag tggaacaacg
    49681 aactggcggg ttggcgtgcg cagttctccc aacaaaccag cgatcgcgag catctgcggc
    49741 aatggcagca acagttaacc catgctgagc aaaaacttaa tgcgcttgcg gcgatcacgt
    49801 tgacgttaac cgccgatgaa gttgctaccg ccctggcgca acatgctgag caacgcccac
    49861 tgcgtcagcg cctggtcgcg ctgcatgggc agattgttcc ccaacaaaaa cgtctggcgc
    49921 agttaatggt cactatccag aatggatctc tggaacagac gcaacgtaat gtcgcactta
    49981 acgaaatgcg ccagcgttat aaagaaaaga cgcagcaact tgccgatgtg aaaaccattt
    50041 gcgagcagga agcgcgcatc aaaacgctgg aagcccagcg tgcacagtta caggcgggtc
    50101 agccttgtcc actttgtggt tccaccagcc acccggcggt cgaggcgtat caggcgctgg
    50161 agcctggtgt taatcagtct cgattactgg cgctggaaaa cgaagttaaa aagctcggcg
    50221 aagaaggtgc ggcgctgcgc gggcaactgg atgcattaac gaagcagctt cagcgcgatg
    50281 aaaacgaagc gcaaagcctc cgacaagatg agcaagcact tactcaacaa tggcaagccg
    50341 tcacggccag cctcaatatc accttgcagc cacaggacga tattcaaccg tggctggatg
    50401 cacaagatga gcacgaacgc cagctgcggt tactcagcca acgacatgaa ttacaagggc
    50461 agattgccgc gcataatcag caaattatcc agtatcaaca gcaaattgaa caacgccagc
    50521 agcagctctt aacggcattg gcgggttatg cactgacatt gccacaggaa gatgaagaag
    50581 agagctggtt ggcgacacgt cagcaagaag cgcagagctg gcagcaacgc cagaacgaat
    50641 taaccgcgct gcaaaaccgt attcagcagc tgacgccgat tctggaaacg ttgccgcaaa
    50701 gtgatgatct cccgcacagc gaagaaactg tggcgctgga taactggcgg caggttcatg
    50761 aacaatgtct cgcattacac agccagcagc agacgttaca gcaacaggat gttctggcgg
    50821 cgcaaagtct gcaaaaagcc caggcgcagt ttgacaccgc gctacaggcc agcgtctttg
    50881 acgatcagca ggcgttcctt gcggcgctaa tggatgaaca aacactaacg cagctggaac
    50941 agctcaagca gaatctggaa aaccagcgcc gtcaggcgca aacgctggtc actcagacag
    51001 cagaaacgct ggcacagcat caacaacacc gacctgacgg gttggctctc actgtgacgg
    51061 tggagcagat tcagcaagag ttagcgcaaa ctcaccaaaa gttgcgtgaa aacaccacga
    51121 gtcaaggcga gattcgccag cagctgaagc aggatgcaga taaccgtcag caacaacaaa
    51181 ccttaatgca gcaaattgct caaatgacgc agcaggttga ggactgggga tatctgaatt
    51241 cgctaatagg ttccaaagag ggcgataaat tccgcaagtt tgcccagggg ctgacgctgg
    51301 ataatttagt ccatctcgct aatcagcaac ttacccggct gcacgggcgc tatctgttac
    51361 agcgcaaagc cagcgaggcg ctggaagtcg aggttgttga tacctggcag gcagatgcgg
    51421 tacgcgatac ccgtaccctt tccggcggcg aaagtttcct cgttagtctg gcgctggcgc
    51481 tggcgctttc ggatctggtc agccataaaa cacgtattga ctcgctgttc cttgatgaag
    51541 gttttggcac gctggatagc gaaacgctgg ataccgccct tgatgcgctg gatgccctga
    51601 acgccagtgg caaaaccatc ggtgtgatta gccacgtaga agcgatgaaa gagcgtattc
    51661 cggtgcagat caaagtgaaa aagatcaacg gcctgggcta cagcaaactg gaaagtacgt
    51721 ttgcagtgaa ataactattc agcaggataa tgaatacaga ggggcgaatt atctcttggc
    51781 cttgctggtc gttatcctgc aagctatcac tttattggct acggtgattg gtagccgttc
    51841 tggtggttgt gatggtggta tgaaaaaagt cattttatct ttggctctgg gcacgtttgg
    51901 tttggggatg gccgaatttg gcattatggg cgtgctcacg gagctggcgc ataacgtagg
    51961 aatttcgatt cctgctgccg ggcatatgat ctcgtattat gcactggggg tggtggtcgg
    52021 tgcgccaatc atcgcactct tttccagccg ctactcactc aaacatatct tgttgtttct
    52081 ggtggcgttg tgcgtcattg gcaacgccat gttcacgctc tcttcgtctt acctcatgct
    52141 cgccattggt cggctggtat ccggctttcc gcatggcgca ttttttggcg tcggcgcgat
    52201 cgtgttatca aaaattatca aacccggaaa agtcaccgcc gccgtggcgg ggatggtttc
    52261 cgggatgaca gtcgccaatt tgctgggcat tccgctggga acgtatttaa gtcaggaatt
    52321 tagctggcgt tacacctttt tattgatcgc tgtttttaat attgcggtga tggcatcggt
    52381 ctatttttgg gtgccggata ttcgcgacga ggcgaaagga aagctgcgcg aacaatttca
    52441 ctttttgcgc agcccggccc cgtggttaat tttcgccgcc accatgtttg gcaacgcagg
    52501 tgtgtttgcc tggttcagct acgtaaagcc atacatgatg tttatttccg gtttttcgga
    52561 aacggcgatg acctttatta tgatgttagt tgggctaggg atggtgctgg gaaatatgct
    52621 aagtggcagg atttcaggac gttattcacc actgcgtatt gcagcagtga ctgactttat
    52681 aattgtactg gcactgctga tgctcttttt ctgcggcggc atgaaaacaa cgtcgcttat
    52741 ttttgctttt atttgttgcg cgggattatt tgccctttca gcaccgctac aaatattgtt
    52801 actacaaaac gccaaaggcg gagagttatt aggtgccgca ggtgggcaaa tagcgtttaa
    52861 cctcggtagc gccgtcggcg catattgcgg tggtatgatg ctgacgctgg ggctggcata
    52921 taattacgtg gcgctgcctg ccgccctgct ttcgtttgct gcgatgtcgt cgttgctact
    52981 gtatggtcgc tataagcgcc agcaagcggc ggatactccg gtgctggcga aaccactggg
    53041 gtaggttata gtctcggtgt tcattactta ttgccggatg cggcgtgaac gccttatccg
    53101 ccctacgcgg ttctggcaca ttttgcaggc ctgataagac gcggcaagcg tcgcatcagg
    53161 catgatgcgc caattgccta cgttttttac tcttgtggcc ataaccacgc agcgccgcgt
    53221 acgccgctgg aatcaccgtg cttcgcctta cgcaccggcg tttcacattc gccgccgaag
    53281 acaaattgtt taatcaactg cggaaccgtt tgatataaac ggtctacatt gctcatc
//
LOCUS       DAJTMK010000006        50353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTMK010000006 DAJTMK010000000
VERSION     DAJTMK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50353
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.006"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(208..414)
                     /locus_tag="OO135_000286"
     CDS             complement(208..414)
                     /locus_tag="OO135_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5396266.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            727..816
                     /gene="kdpF"
                     /locus_tag="OO135_000287"
     CDS             727..816
                     /gene="kdpF"
                     /locus_tag="OO135_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5396267.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            816..2479
                     /gene="kdpA"
                     /locus_tag="OO135_000288"
                     /pseudo
     CDS             816..2479
                     /gene="kdpA"
                     /locus_tag="OO135_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2502..4550
                     /gene="kdpB"
                     /locus_tag="OO135_000289"
     CDS             2502..4550
                     /gene="kdpB"
                     /locus_tag="OO135_000289"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR5396268.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFPRKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGEIFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVGDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4559..5102
                     /gene="kdpC"
                     /locus_tag="OO135_000290"
                     /pseudo
     CDS             4559..5102
                     /gene="kdpC"
                     /locus_tag="OO135_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5095..7779
                     /gene="kdpD"
                     /locus_tag="OO135_000291"
     CDS             5095..7779
                     /gene="kdpD"
                     /locus_tag="OO135_000291"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR5396269.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7776..8453
                     /gene="kdpE"
                     /locus_tag="OO135_000292"
     CDS             7776..8453
                     /gene="kdpE"
                     /locus_tag="OO135_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5396270.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMRHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8669..8926)
                     /locus_tag="OO135_000293"
     CDS             complement(8669..8926)
                     /locus_tag="OO135_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396271.1"
                     /translation="MQKPLPVRWLIIQELTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9049..11247
                     /gene="speF"
                     /locus_tag="OO135_000294"
     CDS             9049..11247
                     /gene="speF"
                     /locus_tag="OO135_000294"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR5396272.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPRISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCNLNSILFLLTPAEYMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11244..12563
                     /gene="potE"
                     /locus_tag="OO135_000295"
     CDS             11244..12563
                     /gene="potE"
                     /locus_tag="OO135_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5396273.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPCFELKNKHG"
     gene            12639..12803
                     /locus_tag="OO135_000296"
                     /pseudo
     CDS             12639..12803
                     /locus_tag="OO135_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="response regulator in two-component regulatory
                     system with KdpD; regulates the kdp operon involved in
                     potassium transport; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            12927..13577
                     /locus_tag="OO135_000297"
     CDS             12927..13577
                     /locus_tag="OO135_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR5396274.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(13618..14112)
                     /locus_tag="OO135_000298"
     CDS             complement(13618..14112)
                     /locus_tag="OO135_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396275.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     GLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(14327..15967)
                     /gene="pgm"
                     /locus_tag="OO135_000299"
     CDS             complement(14327..15967)
                     /gene="pgm"
                     /locus_tag="OO135_000299"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5396276.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(15993..16538)
                     /gene="seqA"
                     /locus_tag="OO135_000300"
     CDS             complement(15993..16538)
                     /gene="seqA"
                     /locus_tag="OO135_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5396277.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            16723..17487
                     /gene="ybfF"
                     /locus_tag="OO135_000301"
     CDS             16723..17487
                     /gene="ybfF"
                     /locus_tag="OO135_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5396278.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            17627..17920
                     /gene="ybfE"
                     /locus_tag="OO135_000302"
     CDS             17627..17920
                     /gene="ybfE"
                     /locus_tag="OO135_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5396279.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            18060..18590
                     /gene="fldA"
                     /locus_tag="OO135_000303"
     CDS             18060..18590
                     /gene="fldA"
                     /locus_tag="OO135_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5396280.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            18800..18886
                     /gene="uof"
                     /locus_tag="OO135_000304"
     CDS             18800..18886
                     /gene="uof"
                     /locus_tag="OO135_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5396281.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            18879..19325
                     /gene="fur"
                     /locus_tag="OO135_000305"
     CDS             18879..19325
                     /gene="fur"
                     /locus_tag="OO135_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5396282.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(19410..19736)
                     /locus_tag="OO135_000306"
     CDS             complement(19410..19736)
                     /locus_tag="OO135_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396283.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKAYCDKVWQE
                     IRSNNK"
     gene            complement(19786..21176)
                     /gene="chiP"
                     /locus_tag="OO135_000307"
                     /pseudo
     CDS             complement(19786..21176)
                     /gene="chiP"
                     /locus_tag="OO135_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(21623..23287)
                     /gene="glnS"
                     /locus_tag="OO135_000308"
     CDS             complement(21623..23287)
                     /gene="glnS"
                     /locus_tag="OO135_000308"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5396284.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(23490..25436)
                     /gene="nagE"
                     /locus_tag="OO135_000309"
     CDS             complement(23490..25436)
                     /gene="nagE"
                     /locus_tag="OO135_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5396285.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            25769..26569
                     /gene="nagB"
                     /locus_tag="OO135_000310"
     CDS             25769..26569
                     /gene="nagB"
                     /locus_tag="OO135_000310"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5396286.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26629..27777
                     /gene="nagA"
                     /locus_tag="OO135_000311"
     CDS             26629..27777
                     /gene="nagA"
                     /locus_tag="OO135_000311"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5396287.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            27786..29006
                     /gene="nagC"
                     /locus_tag="OO135_000312"
                     /gene_synonym="nagR"
     CDS             27786..29006
                     /gene="nagC"
                     /locus_tag="OO135_000312"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5396288.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            29054..29806
                     /gene="nagD"
                     /locus_tag="OO135_000313"
     CDS             29054..29806
                     /gene="nagD"
                     /locus_tag="OO135_000313"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5396289.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            30203..31867
                     /gene="asnB"
                     /locus_tag="OO135_000314"
     CDS             30203..31867
                     /gene="asnB"
                     /locus_tag="OO135_000314"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5396290.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKNILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            32248..32324
                     /locus_tag="OO135_000315"
     tRNA            32248..32324
                     /locus_tag="OO135_000315"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32282..32284,aa:Met,seq:cat)
     gene            32334..32418
                     /locus_tag="OO135_000316"
     tRNA            32334..32418
                     /locus_tag="OO135_000316"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32368..32370,aa:Leu,seq:tag)
     gene            32442..32516
                     /locus_tag="OO135_000317"
     tRNA            32442..32516
                     /locus_tag="OO135_000317"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32474..32476,aa:Gln,seq:ttg)
     gene            32551..32625
                     /locus_tag="OO135_000318"
     tRNA            32551..32625
                     /locus_tag="OO135_000318"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32583..32585,aa:Gln,seq:ttg)
     gene            32641..32717
                     /locus_tag="OO135_000319"
     tRNA            32641..32717
                     /locus_tag="OO135_000319"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32675..32677,aa:Met,seq:cat)
     gene            32766..32840
                     /locus_tag="OO135_000320"
     tRNA            32766..32840
                     /locus_tag="OO135_000320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32798..32800,aa:Gln,seq:ctg)
     gene            32878..32952
                     /locus_tag="OO135_000321"
     tRNA            32878..32952
                     /locus_tag="OO135_000321"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32910..32912,aa:Gln,seq:ctg)
     gene            complement(33106..34281)
                     /gene="ubiF"
                     /locus_tag="OO135_000322"
     CDS             complement(33106..34281)
                     /gene="ubiF"
                     /locus_tag="OO135_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5396291.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGAKSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFSSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            34427..35851
                     /gene="miaB"
                     /locus_tag="OO135_000323"
     CDS             34427..35851
                     /gene="miaB"
                     /locus_tag="OO135_000323"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5396292.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRVARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            36004..37044
                     /locus_tag="OO135_000324"
     CDS             36004..37044
                     /locus_tag="OO135_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5396293.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            37041..37508
                     /gene="ybeY"
                     /locus_tag="OO135_000325"
     CDS             37041..37508
                     /gene="ybeY"
                     /locus_tag="OO135_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5396294.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            37598..38476
                     /gene="corC"
                     /locus_tag="OO135_000326"
     CDS             37598..38476
                     /gene="corC"
                     /locus_tag="OO135_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5396295.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            38501..40039
                     /gene="lnt"
                     /locus_tag="OO135_000327"
     CDS             38501..40039
                     /gene="lnt"
                     /locus_tag="OO135_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5396296.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(40117..41994)
                     /locus_tag="OO135_000328"
     CDS             complement(40117..41994)
                     /locus_tag="OO135_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR5396297.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLTLSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            42449..43357
                     /gene="gltI"
                     /locus_tag="OO135_000329"
     CDS             42449..43357
                     /gene="gltI"
                     /locus_tag="OO135_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5396298.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            43527..44267
                     /gene="gltJ"
                     /locus_tag="OO135_000330"
     CDS             43527..44267
                     /gene="gltJ"
                     /locus_tag="OO135_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5396299.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            44267..44941
                     /gene="gltK"
                     /locus_tag="OO135_000331"
                     /pseudo
     CDS             44267..44941
                     /gene="gltK"
                     /locus_tag="OO135_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
     gene            44941..45666
                     /gene="gltL"
                     /locus_tag="OO135_000332"
     CDS             44941..45666
                     /gene="gltL"
                     /locus_tag="OO135_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5396300.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            45784..46719
                     /gene="rihA"
                     /locus_tag="OO135_000333"
     CDS             45784..46719
                     /gene="rihA"
                     /locus_tag="OO135_000333"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5396301.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            46803..48497
                     /gene="hscC"
                     /locus_tag="OO135_000334"
     CDS             46803..48497
                     /gene="hscC"
                     /locus_tag="OO135_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5396302.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLKDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIPLTRLPDAA"
     gene            complement(48533..49984)
                     /gene="djlC"
                     /locus_tag="OO135_000335"
                     /pseudo
     CDS             complement(48533..49984)
                     /gene="djlC"
                     /locus_tag="OO135_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
     gene            complement(49981..>50353)
                     /locus_tag="OO135_000336"
     CDS             complement(49981..>50353)
                     /locus_tag="OO135_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5396303.1"
                     /translation="GIKAWDYVRMGVLSRVGVLNKWLTEEESLWLQSRVYVRAHHYYH
                     GWMHYFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATL
                     PWQPLTVQPECPVTLKDVSDL"
BASE COUNT        12150 a        12869 c        12903 g        12431 t
ORIGIN      
        1 gctgacaggc aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata
       61 gaaacaatta ggaaaatatt tatatatttt tcctgaatat atttcagctg ggtaatacca
      121 tatctaaata tttacctgaa catcttccta ataaaaaaac cgccagcatc tccactagcg
      181 gttttcaatg tattcaaaac tcggcaatca gtaaaaatca ccggttgcct tttcggcctg
      241 ttccatccaa accggtttat cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa
      301 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      361 atatgtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      421 ctcccccttt tgtatctacc cggtgaatag caccggaaaa atgaatttgt ttatctgatg
      481 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      541 gatggtttat ttacattcac tctgcaatta agtcacacaa aagttaaatt aatgctaaac
      601 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      661 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
      721 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
      781 ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      841 ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      901 ggctgattaa tgacattcct cttccaggta cagcgggcgt tgaacgtata ctctttcgtt
      961 tacctggcgt ctctgaccat gagatgaact ggaagcaata tctttgtgcc attctcggcc
     1021 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
     1081 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     1141 ttgtcacgaa taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc
     1201 agatggcagg cttaacggta caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1261 ttgccttcat ccgtgcgttt actcgccaga gcatgagcac actcgggaat gcctgggtcg
     1321 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcactgtttt
     1381 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcagtgaat accgttgaag
     1441 gagcgcaaca gctgttaccc atgggaccag tagcttctca ggaagcgatc aagatgctcg
     1501 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1561 cactgaccaa cttcgtgcag atgctggcat cccaacagct ctgtgctttg cctttggtga
     1621 agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt
     1681 catctgcgta ggcgtggtta tgtgggcaga agttcagggt aatcctcatc tgctggcgct
     1741 cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag
     1801 tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg cgatgcatga
     1861 ttcgtttacc gctctcggcg gcatggtgcc gatgtggcta atgcaaattg gtgaagtggt
     1921 gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt
     1981 tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg
     2041 cgagatgaaa ctgactgcac tggcgattct ggttactcca acactggtac tgatgggcgc
     2101 ggcgttggcg atgatgaccg acgccggacg tagcgccatg cttaaccctg gcccgcatgg
     2161 ttttagcgaa gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca gcgcctttgc
     2221 cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg
     2281 tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag
     2341 ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg gcccgttaat
     2401 cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt
     2461 ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg
     2521 cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc
     2581 cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca
     2641 cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg tttagcgcgg
     2701 caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag
     2761 aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact gcctttcccc
     2821 gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc gatcaactgc
     2881 gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat ggtgaagtca
     2941 ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc
     3001 gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc
     3061 tggtgattga gtgtagcgtt aaccccggtg agatatttct ggatcggatg atcgcgatgg
     3121 tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc ctgctgattg
     3181 ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg
     3241 gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta
     3301 ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg
     3361 tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctactgg
     3421 ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc cctgcgcagg
     3481 gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc
     3541 cggaaggccg cagtattgtg atcctcgcca agcaacgttt taacctgcgc gagcgcgatg
     3601 tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca
     3661 acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg
     3721 ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg
     3781 gggccacgcc gctggtggtg gtggaaggtt cccgtgtgct gggcgttatt gcgctgaaag
     3841 atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg ggcattaaaa
     3901 cggtgatgat taccggcgat aaccgtctga ctgccgccgc cattgctgcg gaagcgggcg
     3961 tcggtgattt tctcgccgaa gcgacaccgg aggccaagct ggcattgatt cgtcagtatc
     4021 aggcggaagg ccgtttggtg gcgatgaccg gcgacggcac caacgatgct ccggcgctgg
     4081 cgcaagcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa gaggcgggca
     4141 atatggtcga tctcgactct aacccgacca agttgatcga ggtggtgcat attggcaaac
     4201 agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat
     4261 acttcgccat tattccggcg gcgttcgcgg caacgtatcc gcagttaaat gcgctgaaca
     4321 tcatgcgcct gcattcgccc gactccgcca ttctcagtgc agtcattttc aacgccttga
     4381 ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt
     4441 ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta
     4501 ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga ggtttaccat
     4561 gcgtggctta cgtccggcat tatcgacatt tatctttctg ttattgatta ctggcggcgt
     4621 ttacccgctg ctgaccaccg tgctggggca atggtggttt ccctggcagg ccaacggttc
     4681 gttgattcgt gaaggtgata cggtgcgggg ttcggcatta atcgggcaga attttaccgg
     4741 caacggcaga aatgccctat aatccacagg cttctggcgg gagcaatctg gcggtcagta
     4801 accctgagct ggataaacaa atagccgcac gcgttgctgc attacgggcc gctaacccgg
     4861 atgccagtac gaacgttccg gttgaactgg tgacggcatc ggcaagcggg ctggacaata
     4921 atatcacccc gcaagcggcg gcctggcaga tcccacgcat cgcgaaagcg cgtaatctca
     4981 gcgttgaaca gctcacgcag ctgatcgcaa aatacagcca acaaccgctg gtgaaatata
     5041 tcggccagcc ggtcgtcaac attgtcgaac tcaatctggc gctggataag cttgatgaat
     5101 aacgaaccct tacgtcccga ccccgatcgt ctgctggaac aaactgccgc gccgcatcgg
     5161 gggaagctga aagttttctt cggtgcctgt gcaggcgtcg ggaagacctg ggcgatgctg
     5221 gcagaagccc agcgactgcg ggcgcaaggg ctggatattg tggttggcgt ggtagaaacc
     5281 cacgggcgaa aagataccgc cgcaatgctg gaagggctgg ctgttctgcc gccaaaacgc
     5341 ctggcgtacc gtgggcggca catctgcgag tttgacctcg atgccgccct cgcccgccgt
     5401 ccggcgctga tcttaatgga cgaactggcg cacagtaatg cgccaggttc tcgtcaccct
     5461 aaacgctggc aggacatcga agagctgctg gaagcaggca ttgatgtttt cactaccgtc
     5521 aacgttcagc atctggaaag tctgaatgat gtggtcagcg gcgtcaccgg aattcaggta
     5581 cgggaaaccg tgcccgatcc ttttttcgat gccgccgacg acgtggttct ggtggacttg
     5641 cccccggacg atctgcgcca gcggctgaaa gaaggcaaag tctatattgc cgggcaggcg
     5701 gagcgcgcca ttgaacattt tttccgcaaa ggcaatctga tcgccctgcg cgaactggca
     5761 ctgcgccgta ctgccgatcg cgttgatgag caaatgcgcg cctggcgggg gcatcctggc
     5821 gaagagaaag tctggcacac gcgcgacgcg atccttttat gcatcggcca taacaccggt
     5881 agtgagaagc tggtccgcgc ggcggcgcgg ctggcgtcac ggctgggtag cgtctggcac
     5941 gcggtgtatg ttgaaacccc tgccctgcac cgcttaccgg aaaaaaaacg tcgggcaatt
     6001 ctcagcgcct tacgtctggc gcaggagctg ggcgcggaga cggcaacact ttctgatcca
     6061 gcggaagaaa aagcagtagt gcgttatgcc cgtgaacata atctcggcaa gattattctc
     6121 ggtcgcccgg cctcgcgccg ctggtggcgt cgggaaacgt ttgctgaccg actggcgcgc
     6181 atcgcccccg atctcgatca ggtgctggtc gcgcttgatg aaccacccgc acgcacgatt
     6241 aacaacgcgc cggatagccg ctcttttaaa gacaagtggc gggtacagat tcagggatgc
     6301 gtggttgccg ccgcgttatg cgccgttatc actttaattg ccatgcagtg gctgatggcg
     6361 tttgatgccg ccaacctggt gatgctgtat ctgcttggcg tggtggtggt ggcgctattt
     6421 tatggacgct ggccttcggt ggttgctacc gtcattaatg tggtgagttt cgatctcttt
     6481 tttatcgccc cacgcggcac gctcgccgtc tctgatgtgc aatatctgct gaccttcgca
     6541 gtgatgttaa ccgtcgggct ggtgatcggg aaccttactg ctggcgtgcg ttatcaggcg
     6601 cgggtagccc gttaccgcga gcaacgcaca cggcacttat atgaaatgtc gaaagctctg
     6661 gcggtgggcc gcagtccgca ggacatcgcc gccaccatcg aacaatttat tgcctccacg
     6721 tttcatgccc gcagtcaggt gttgttgccc gatgacaacg gtaaattgca gccgttaaca
     6781 catccgcaag gaatgacgcc atgggacgat accatcgcgc agtggagttt tgataaaggc
     6841 ctgcctgcgg gcgcgggcac cgacacgtta cccggtgtac cgtaccagat tttgccgcta
     6901 aaaagcggcg agaaaaccta cgggctggtg gtggtggagc cggggaatct gcgccagttg
     6961 atgatcccgg aacagcagcg cctgctggag acgtttacgc tgttagtcgc caatgccctt
     7021 gagcggctga cgctaaccgc cagcgaagaa caggcgcgga tggcaagcga acgtgaacag
     7081 atccgcaacg ccctgctggc agcgctttcg catgatttac gcacgccgct cacggtgctg
     7141 ttcggtcagg cagaaatctt aacgctcgat ctggcaagcg aaggatcgcc ccacgcccgc
     7201 caggccagcg agattcgtca gcatgtgctg aacactaccc gactggtgaa taatctactg
     7261 gatatggcgc gaattcagtc cggcggcttt aatttgaaga aagagtggtt aacgctggaa
     7321 gaagtggtcg gtagcgcgct gcaaatgctg gaaccgggtt tatcgtcgcc catcaatctt
     7381 tctctgccag aaccgctgac cttaatccac gttgacgggc cactctttga acgggtgctg
     7441 attaatctgc tggagaacgc ggtgaaatat gcgggtgcgc aggccgaaat tggtatcgat
     7501 gcccacgttg agggcgaaaa tctacaactg gatgtctggg ataacggccc cggtcttccg
     7561 ccaggccagg agcagacgat atttgataag tttgctcgcg ggaataaaga gtcggcagta
     7621 ccgggggtag ggcttggact ggcaatctgt cgggcgatag tggatgtaca cgggggcact
     7681 attaccgcgt tcaaccgacc ggaaggtggt gcctgttttc gtgttacact tccccagcaa
     7741 actgcccctg aacttgaaga atttcatgag gatatgtgac aaacgttctg attgttgaag
     7801 atgaacaggc tattcgtcgc tttctgcgca cggcgctgga gggcgacggg atgcgcgtct
     7861 atgaggccga aacgctgcaa cgcggcttgc tggaagcggc tacccgtaag ccagatttga
     7921 ttattctcga tctcggcctg cccgatggtg atgggattga gtttatccgc gacctgcgcc
     7981 agtggagcgc ggtgccggtg attgtgcttt ccgcacgcag cgaagagagc gacaaaatcg
     8041 ccgcgctgga tgccggagcg gatgattatc tgagtaagcc gtttggcatt ggcgaattgc
     8101 aggcccgtct gcgcgtcgct ttacgccgcc actccgccac cgccgcgccc gatccgctgg
     8161 tgaaattttc cgatgttacc gtcgatttag ccgcccgcgt gattcaccgg ggtgaggaag
     8221 aggtgcatct cacaccaatt gagttccacc tgctggcggt gctgctcaac aatgccggaa
     8281 aagtactcac ccagcgccag ctccttaacc aggtgtgggg gccaaacgcg gtcgaacaca
     8341 gtcactattt gcgtatttat atgagacatc tgcgacaaaa actggaacag gatcccgccc
     8401 gcccacgcca tttcattact gaaaccggta ttggctatcg gtttatgctt tgaatattaa
     8461 ttttaataca gcctgccttt tattaattaa agccgtaata ataaatacgg ctttttatct
     8521 taaacaacac acaaaaataa caattcaata ttttatatta ctgagtaaaa agctcatcat
     8581 taaataaatt aagattgatc attttttatt gatcaccttc acagttcaac cgtatttcct
     8641 ggatagaata tcttcacctt cattcacatc aggaaaggta aattaaatgg aaaataacag
     8701 ccgcactatg ccccatataa ggcggacaac tcatattatg aagtttgctc atcgcaatag
     8761 cttcgacttt cacttcttca atgcccgtta gtctaccgac taagggcact tcagcgtaca
     8821 ggtcttcctg actctctgta ttacaggcat atgcctgtat tccgcagtgc tcattctctg
     8881 ttaactcttg tattatcagc caccggactg gtaagggttt ctgcattctg aaaaaagcaa
     8941 tctacctgat ttatttatca gcggggtaac tttgcttttt tccggcacga tcgatttctc
     9001 attcgagaaa ttgaggacct gctattacct aaaataaaga gatgaaaaat gtcaaaatta
     9061 aaaattgcgg ttagtgattc ttgcccggac tgttttacca cgcagcgaga atgtatctac
     9121 attaatgaaa gtcgtaatat cgatgtggcg gcaatcgttt tatcgctcaa cgatgttaca
     9181 tgcggaaaac tcgatgaaat cgatgccacg ggttatggca tcccggtatt tattgctact
     9241 gaaaatcaag aacgtgtacc cgcagagtat ttgccccgta tttcgggtgt ctttgagaat
     9301 tgcgaatcgc gacgagaatt ttatggtcgt cagttagaaa ccgctgccag ccattatgaa
     9361 actcaactgc gcccaccttt cttccgcgca ctggtcgatt atgtcaatca aggtaacagc
     9421 gcgtttgatt gccctggtca tcagggcggc gaatttttcc gtcgccatcc ggcggggaat
     9481 cagtttgtgg aatactttgg tgaggcgctg ttccgttccg acttgtgcaa cgcagacgta
     9541 gcaatgggtg atctgctgat tcacgaaggc gcgccatgca ttgcacagca acatgcggca
     9601 aaagtgttca atgccgataa aacatacttc gttctaaatg gcacttcatc ttctaacaaa
     9661 gtggttttaa acgccctgct gacaccgggt gatctggtgc tgtttgaccg caataaccac
     9721 aaatctaacc accacggagc gttgctacag gctggtgcaa caccggttta tctggaaacc
     9781 gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga agaaagttac
     9841 ctgcgtgagc tgatcgcgga agtcgcaccg cagcggacaa aagaggctcg tcctttccgc
     9901 ctcgccgtta ttcagctagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg
     9961 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt cggctatgag
    10021 caatttatcc cgatgatggc tgactgttca ccgctgttgc tggatcttaa tgagaacgat
    10081 ccgggcattc tggttacgca atctgtgcat aaacagcagg ctggtttttc tcaaacttca
    10141 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
    10201 atgaacaacg cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca
    10261 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
    10321 gtggtaaatg gcattaatgc ccgcaagctg atcctcgata actgtcagca tattcgtccg
    10381 ttcgtacctg aactagtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
    10441 gttgatctgc gcttcttcca gtttgtacca ggggaacact ggcactcttt tgaaggctat
    10501 gcagagaatc aatactttgt cgatccatgc aaactgttgc tgacaacccc aggtattgat
    10561 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
    10621 cgcgaaaatg gcgtagtgcc ggaaaaatgc aatcttaact ccatcctctt cctgctgact
    10681 ccggcagaat atatggccaa acttcagcaa cttgttgccc tgctggtacg cttcgaaaaa
    10741 ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa acagcatgaa
    10801 gagcgctacg ccggttatac ccttcgtcag ttgtgccagg aaatgcatga tttgtatgcc
    10861 cgtcacaacg tgaaacaacc gcaaaaagag atgttccgta aggaacactt cccacgcgta
    10921 agcatgaatc cacaagaagc caactacgcc tatttacgcg gtgaagtgga gctggttcgt
    10981 ctgccggatg ccgaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg
    11041 ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg
    11101 gaagaaggga tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa
    11161 gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc
    11221 accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc
    11281 agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa
    11341 agcttgccga agttgggaca atctcaatta tctcctggct ggtgacagcc gtaggctcaa
    11401 tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg
    11461 gcggttatgc ggagtatgct ttcggtaagt ccggcaattt tatggcgaac tacacctacg
    11521 gtgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg
    11581 aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggagtgctgt
    11641 ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta
    11701 ctgtgtgggg agtcattatt ccggtcgtcg gtctgtgcat tattggctgg ttctggttta
    11761 gtccgacgct gtacgctaac tcatggaacc cgcaccatgt gccgttcttt agcgctgtag
    11821 gttcttccat cgccatgacg ttgtgggctt tcctcggtct ggagtcagcc tgtgccaacg
    11881 ccgaagtagt ggaaaacccg gaacgtaacg tgccgattgc agttcttggc ggtactttag
    11941 gtgcggcggt gatttatatc gtctccacca acgtgattgc cgggattgtg cctaatatgg
    12001 agctggcaaa ttcaacggca ccatttggcc tggctttcgc acagatgttc acaccagaag
    12061 tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcactg cttggctggc
    12121 agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt
    12181 tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattattc
    12241 agagtggatt ggcactgatg accattagcc cgtcactgaa cagccagttc aacgtgctgg
    12301 ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca
    12361 ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agtagcaaac tttgttgcct
    12421 ttgttggtgc gatgtacagt ttctatgcgc tgtactcatc cggggaagaa gccatgctgt
    12481 acggttctat cgtgaccttc cttggttgga cactgtatgg tctggtatca ccatgctttg
    12541 aactgaaaaa taaacacggt taataaaaca aagtaaaaaa gggcgattga atgatcgccc
    12601 tttctgtttg ttggcaaata gttcgttatt tatgccgcat ccggcagatg tagggtctaa
    12661 acgcgatcga acacagcaac tatttgcgta attatatggg gcatctgcga caaaaactgg
    12721 aacaggatcc ggcccggccc ggcccggccc ggcctcgcca ttttattact gaaaccggta
    12781 ttgggtatcg gtttatgctt tgaaaaaagt ggcaatgtat ggataaagtc attttttatt
    12841 caaatataag gaacttaata tttaaaaaat gttccataca attcctcatc ataaatgaca
    12901 ccgtagcaca caaggagcaa gtagcgatgc gtgaaatgaa tcagttagta aatactgaag
    12961 attcagcctg gccaataatc cagaactggt taaaagacgc cacaaaccat accgaactct
    13021 taccggttaa taaaaattta gccgaaaccg cactttatca attacaggtg acaacaaaat
    13081 caccgatggg tgcgcttgtc tatggctccg gaggtctgct gattgataat ggttggttac
    13141 gcatagcagg atctggtcac ccccggttgc cacgagatcc agtaagttgg acacaacgcc
    13201 ctgaattcgc aggcgtgcgg gctttaccca tcgctgacga tgtcgcaggg ggaatttttg
    13261 ctcttaatgg tggcgatctt ggggaagata ctggctgcgt ttattatttt gcgccagata
    13321 cgctgaattg ggaaagtctg gaagttggat attctgagtt tcttcagtgg gcattatccg
    13381 gtgatttaga taccttctat gaaaatgtac gatggcagca atggcgagaa gacgtaataa
    13441 aattatcggc aacggaagcc ttcacgttct accctttctt atgggtacaa agtgaagaag
    13501 caagaacaag aaaagttatt tcgcttactg aactctggga aatgcagtat caaatgaaag
    13561 aaacattcac gcaataagac atgttcaggt ccgggtttca ttcccggact cactatttta
    13621 atgtgtattt atcgctttca attcttgctg gcaattttct gactggcagc gtcgtaactc
    13681 aataatcccg acatgcatca tcgagccaaa aaatccgctt tcgctgacat aatcctcatt
    13741 gactatcggt cgagtgccgg gcttgccctg gcaaacccgc ataaaaaatg aggtgtagca
    13801 ttgccaaccg ggatttaaac cgaccttttc ttgtgctgac atattatgaa gataagtaag
    13861 ttctttttcg ttcccgcctg acaactgcca gcggcgggta ttatcaggta agttatcacc
    13921 acggcaattt gtcttacacc gggaaggtgc aatatttacc gcaatgctac ccgttaatcc
    13981 tctggtttta tctttagggg tcagtaacca gtcgccagct aatgatgatt gttcaggttg
    14041 ggcgcatgca gtcagcagta atgtggcggt aattagtgac actatgacga ccagactcct
    14101 gttggtcttc attgggattt ccttgagcta ctcagtttgt tgacaaagtg ttcgttgttt
    14161 ttgccggatg cggcgtgaac gccttatccg gcctacaaaa tctcgtaaat tcaatatatt
    14221 gcaccatcct cgtaggcctg ataagcgtag cgcatcaggc aattctgtgt ttgtcatacg
    14281 taaaaaaggg cgatcttccg accacccttt ttcttattta atgtgtttac gcgtttttca
    14341 gaacttcgct aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga
    14401 ggaagctttc gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc
    14461 agccgttgtc agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg
    14521 tcaggcgcgc ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca
    14581 gcttagacag cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg
    14641 gcgcaccaaa gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag
    14701 cggtgatttc cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg
    14761 tgccgtcgaa acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc
    14821 cgtcgaacag accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc
    14881 ccaagtcatt gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa
    14941 catctttgcc ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt
    15001 ggttcggatt catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt
    15061 tagcaaacgc cagatcgaac ttatcacgca gtgccagcag gcccgccatc gcacactcgg
    15121 aggagcagtc catacggatc gcgccgtctt tatcaaggtg cataaagcgg aaggtttgat
    15181 cgacctgatc gttaacgatg gtcaggttga ggttgtaata ctcgccaata cgcttccagt
    15241 attcgatacc ggaaccgccc agcggatcaa cgcccagcgt caggcccgct ttctgaatcg
    15301 ccgccatatc aacgatatcg gccagacctt ccacaaacgg ctgcaccaga tcctgctctt
    15361 tcacatgacc ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat
    15421 cggccagcag tgcgttggcc ctgtcttcca ccactttggt gacgttggta tcagccgggc
    15481 caccatttgg cggattgtac ttgataccac catcttccgg cgggttatgg gacggtgtaa
    15541 tcacgatacc gtctgccagc ggaccacctt ttttattgtg aaccaggatg gcattggaaa
    15601 cggcaggcgt tggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg
    15661 ccagcacttc cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca
    15721 catagcaagg gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg
    15781 ccagaatgtg cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac
    15841 cgaatttcac cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg
    15901 tcagttgggc gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga
    15961 ttgccattgc tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc
    16021 aattccgccg ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg
    16081 gtgttggtgt tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga
    16141 ttaccatttt tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc
    16201 aacgattccg ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc
    16261 agcataaagc gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca
    16321 cgcattgcgc gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt
    16381 gacgcaacgc gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc
    16441 aacatacgcc gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata
    16501 tagctgtaga gttcatcatc aacttcaatc gttttcatct taatccagtg cagtgtctta
    16561 gctgaataca agtcgccaag agtatatgaa taaatcccgg cagcggattg cgtcaagcct
    16621 ggcttgcaga aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa
    16681 cataataccc taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc
    16741 gcgcaaactg cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt
    16801 ggtagcctcg acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc
    16861 caggttgata tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg
    16921 atggcgcagg atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc
    16981 ggtcactcca tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc
    17041 gataaactgg tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag
    17101 attttcgcgg ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca
    17161 gcaataatgc gccagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt
    17221 gttgacgggg agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg
    17281 ggttgggaga aaatcccggc atgggatcac cctgccctgt ttatccctag cggcaattct
    17341 ccgtatgtta gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg
    17401 catgtgattg caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc
    17461 gctatccgtc gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc
    17521 tttccgcgcg tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat
    17581 ccgggcagag gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca
    17641 aacggaccgt acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac
    17701 taatccgctt tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga
    17761 caaagtacgt ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct
    17821 gaacgagctg gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat
    17881 gcagcaactg gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca
    17941 cagtgtgcag tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc
    18001 tttatccgtg ggcaatttcc cccccccatt tcaataagtt tcaagaggtt atttcactca
    18061 tggctatcac tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa
    18121 tgattcaaaa acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca
    18181 aagaagatct ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg
    18241 aagcgcagtg tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca
    18301 aactggttgc gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg
    18361 cattgggcac catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc
    18421 caactgcggg ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg
    18481 gtctggctat cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg
    18541 ttaaacagat ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg
    18601 cgtagactca tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat
    18661 ttgtaacttt tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt
    18721 aaatgtaagc tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt
    18781 taggcgtggc aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc
    18841 taatgaagtg aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc
    18901 taaagaaagc tggcctgaaa gtaacgcttc ctcgtttaaa aatcctggaa gttcttcagg
    18961 agccggacaa ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg
    19021 aagaaattgg tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg
    19081 tcacccgcca caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc
    19141 acgatcacct gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg
    19201 aagcgcgtca gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct
    19261 atctttacgg tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca
    19321 aataagccag cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa
    19381 ataagaaaag cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa
    19441 actttatcac aataggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac
    19501 tgctgataat ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct
    19561 ttcttcagca cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg
    19621 gtgttgatac aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat
    19681 tgcgcacaag ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca
    19741 aattcctcaa acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa
    19801 tggtgcgatt accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc
    19861 gccccagctt gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt
    19921 gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc
    19981 aatagtccgg tttttgtcgt agtacgcatc cggattgctc tgccaggtcg caggtttagc
    20041 atcccatgcg taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag
    20101 gtcatacatc gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt
    20161 atcccaccag atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag
    20221 gaagtatccc tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac
    20281 gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc
    20341 gttgacgctg cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa
    20401 cgggctaccg gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc
    20461 ttccgcctga ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc
    20521 aagggagtgc agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc
    20581 cagatgccac ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc
    20641 atcgccgtaa tcaaaattcg ccccggcttc cgccccctga taagtacctg cggcgctaac
    20701 agcgtttgac cagttggctg aatgtaacct gccctcgccc aaaccggacc gtatttaaat
    20761 ttggccgcag ctttatacag gcttataccg cttttgtcgc cggaccagtc ttcgtcatag
    20821 gctttattac tttttgaaaa cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc
    20881 atttcaatcg ccgtaaacac agcaatatca aggccgaaca tatcagcagc ataaccggac
    20941 tgaaaatcga ggttggcatt ccaggtggag tgagaaaggt tggttttgta tttgtcgccg
    21001 tcggtaacat ctttacggtc gcgttcacgc tgccagtaat atataccgcc ggttaaggtt
    21061 gaatcgtcga tgaatccttc ggcgcgagcc tccggcattg ccataaagcc cgacattgct
    21121 gtaacaccgg cgatagccag cgccagcgta ctacgtttgc cactaaacgt acgcatgggt
    21181 taatcctctt tgacgtataa attgctgcac caaaggtgaa cagcgaaaaa aataaaaatt
    21241 aaaaaagtag cattgagaaa agtctcgctg actacaggaa tagactattt ttcgcgacgc
    21301 gaattatcaa tctctttctg taaccagaat gtaagaacat gagcaaccgc acatattttg
    21361 atgatttttg ttacaaggct taagtttgcg gttgaaaggc ttgcagaaga ataaaagcgt
    21421 aaatttccag caagatgaaa gcggttacat tttatttaat tatggtttgt gattttaaat
    21481 ttattttatg gataaattgc gaagcggaat taattcgcga agaaaagcag aaaaaacgcc
    21541 gcagagtgcg gcgtttggga ttgtttgcta tttcttgccg gatgcgacgt aaatgcctca
    21601 cccggcctgg tagaacttaa aattactcgc ctactttcgc ccaggtatca cgcagcccaa
    21661 cggtgcggtt aaataccggt ttttccgctg tggaatggcg gctgtcgagg cagaagtaac
    21721 cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac ggttcagcaa
    21781 agccctgttt gatcaccagc gattccgggt taatcaccga caggaaatca tccgcagcac
    21841 ctgggttagg cacgctgaac agacggtcgt acagacggat ttcaaccggc agcgcatgtg
    21901 ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga tctttgctta
    21961 aggtgtcggc gtcataagta cagaagatgg tggtgatatt gccttcggca tctttctcca
    22021 cgcgttctgc cttaatgacg taagcattac gcagacgcac ttctttaccc agcaccagac
    22081 gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa atctcaccgc
    22141 taaacggcac ctggcggcta cccatttccg gtttgttcgg atggttcggc atggtaacca
    22201 tttcgccttc gccctgatag ttttcgataa ccagtttcac cggatcgata accgccattg
    22261 cgcgcggggc gttttcgttg agatcttcac ggatgcagga ttccagcgac gccatctcaa
    22321 tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata gaagcagcag
    22381 tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc cagccttcaa
    22441 cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga catcacagtg tattccagat
    22501 tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg tccagcaccc
    22561 agtcgtacag gcgacggttg tcctggaact caagcgtaca cagagagtgc gtaatacctt
    22621 ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac cacttgttgc
    22681 cagtctggtg gtgttcagca aatttaatgc ggtacagcac cggatcgcgc atcacgataa
    22741 acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt accttcttca aaaccaccgg
    22801 cacgcatttt ttcgaacagc gccaggttct cttcaacgct gcggtcgcgg tacgggctgt
    22861 ttttacccgg ttgcgtcagg gtgccgcggt attcgcggat ctgttccggc gtcagttcat
    22921 caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc tgatcaaaat
    22981 aatcggagga gtaacggacg ttaccagacc agtgaaaacc taaccactct acgtcgtttt
    23041 tgatcgactc aacatactcg atatcttctt ttaccgggtt agtgtcgtcg aaacgcaggt
    23101 tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat ttcgcatggc
    23161 caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg gtgtgcttac
    23221 cactggccag atcttcatcg atgatctgac ggataaagtt agtcgggcgg gcttctgcct
    23281 cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat cttataagcg
    23341 ggacaggctg acaactgtta gttttttaaa attgataatc taacaaggat tatggggatg
    23401 attgatgaaa aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg
    23461 ctcaagcgtc gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga
    23521 cccgccacaa catggccctg agctttaatg atcaagccac tgaaatcgtc gatattgctg
    23581 caaaccaccg ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc
    23641 agaatcggtt gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta
    23701 ccttccagcg ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt
    23761 tccaggcaga acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat
    23821 acgacgattt tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat
    23881 gcttcgtcag gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc
    23941 gcgatagata ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgacgga
    24001 gttgcttcag ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg
    24061 ccgatggatt ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact
    24121 acaccagaag cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg
    24181 gtcaggcgca gacgggtgat acaggcgtca attgctttca ggttgtcagt gccgccaacc
    24241 gcagcaatat agttggtcgc cagttgattg aggccttctt cagtgttgct gttggcttct
    24301 tcagtaacga tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg
    24361 ataaccaaac tgaacaccac gaagtagata gcgaagaaga caacacccat caccagcagc
    24421 atccagacgt tctggctggc ggccggcagg ttatacatca acgcgtagtc gatagccccc
    24481 gcagagaaag agaagccagc atggataccc agcagtgttg ccacaaacag gctgataccg
    24541 gtcagcagtg cgtgcaggag gtacagcagc ggtgcaagga acatgaacag gaattccagc
    24601 ggctcagtca caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc
    24661 atcggacgac gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg
    24721 aacatcataa tcgggaagaa gccggacatg aacatccccg cggtgccgtc accggcatag
    24781 aagcggttga tatcaccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg
    24841 aaccaggcga tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg
    24901 aaaccaaaga taccggaacc cagcgcgccc gcagaaacga tccactcgcc tcctgcatgg
    24961 atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag
    25021 aagccggtgg caatcggcac aaaacgtttg ccgccgaaga agctcaggaa gtccggcagt
    25081 ttaatatcgg accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc
    25141 agtacaccca tgtttatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa
    25201 cctaccgcac ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca
    25261 ccgatggcga agattaatgc gaggttatca aaaatcgcac cgcccgcctg ggcaataaac
    25321 gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca gtgccgccac cggcagcacc
    25381 gcgataggga gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta
    25441 ttccccctac gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat
    25501 tgtactgccg atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat
    25561 ttaattcgta tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta
    25621 tcatcactcc cttttactgg ctaaaccaga aaacttattt tatcattcaa aaaatcaggt
    25681 cggattgacg cctgtctgcg caaatccaag ttacgcttaa agatacctaa tccgccaacg
    25741 gcttacattt tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca
    25801 ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccgactgc
    25861 cgatcgtccg tttgttctgg gcctgccaac tggcggcacg ccgatgacca cctataaagc
    25921 gttagtcgaa atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat
    25981 ggacgaatat gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg
    26041 taatttcttc gatcacgttg atattccagc agaaaacatc aaccttctca acggcaacgc
    26101 cccggatatc gacgccgaat gccgccagta tgaagaaaaa atccgttctt acggaaaaat
    26161 tcatctgttt atgggcggtg taggtaacga cggccatatt gcatttaacg aaccggcgtc
    26221 ttctctggct tctcgtactc gtatcaaaac cctgactcat gacactcgcg tcgcgaactc
    26281 tcgtttcttt gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg
    26341 tacattgctg gatgccgaag aagtgatgat tctggtgctg ggtagccaga aagcactggc
    26401 actgcaggct gcagttgaag gttgcgtgaa ccacatgtgg accatcagct gtctgcaact
    26461 gcatccaaaa gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac
    26521 tttaagatat ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct
    26581 gccctggctc cttgctcagg gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta
    26641 acccagggcc ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct
    26701 gatggcctga ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt
    26761 tcactgaacg gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc
    26821 ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag
    26881 aaagccaatg agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat
    26941 gagctgatga aacagggcgt gcgcgttatg cgcgagtacc tggcaagaca tccgaatcag
    27001 gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat
    27061 ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac
    27121 gtcattacca aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg
    27181 gcaaatgccg ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcgaaa
    27241 gccggtttcc gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt
    27301 accggtcgtg aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt
    27361 attatcgctg atggcctgca tgttgattac gccaacattc gtaacgctaa acgccttaaa
    27421 ggcgacaaac tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag
    27481 ttcatttttg cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt
    27541 acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc
    27601 ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattggc
    27661 gttgagaaac gtctcggcac actcgccgca ggtaaagtag ccaacctgac cgcattcaca
    27721 cctgatttta aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag
    27781 aaagtatgac accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta
    27841 acagcgcggc ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg
    27901 ccgagcaaag ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac
    27961 gcgggctgat caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca
    28021 tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca
    28081 ccatcaccct gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg
    28141 aacgtaccca gcaaacgctg gaacatgccc tgttgaatgc cattgctcag tttattgata
    28201 gctaccagcg caaactgcgc gagctgatcg cgatttcggt gatcctgcca gggcttgttg
    28261 acccggacag cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg
    28321 tagaagctct ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc
    28381 tggcgctggc ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc
    28441 gtgtccatcg cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca
    28501 acggtaacgt cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact
    28561 gcggcaactt tggctgcctg gaaactatcg ccgccaacgc tgccattgaa caacgggtgt
    28621 tgaatctgtt aaagcagggc taccagagcc gcgtgccgct ggacgactgc accatcaaaa
    28681 ctatctgcaa agccgcgaac aaaggcgata gtctggcgtc ggaagtaatt gagtatgtcg
    28741 gtcgtcatct gggtaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg
    28801 ttattgccgg tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca
    28861 ttaataccca ggcgctgaag gcgtttcgca ctaatctgcc ggtggtacgt tctgagctgg
    28921 atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt
    28981 tgctccagca tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata
    29041 ttctgggtag tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca
    29101 cgataacgtc gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct
    29161 gccgctggtg ttgctgacca actatccttc gcagactggg caagatctgg cgaaccgctt
    29221 tgccaccgca ggtgtcgatg tacctgacag cgtgttttat acctctgcga tggcgactgc
    29281 cgatttcctg cgtcgccagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat
    29341 tcatgaactg tacaaagccg gtttcactat taccgatgtg aaccctgatt tcgtgattgt
    29401 tggcgaaacg cgttcctaca actgggacat gatgcataaa gcagcctatt tcgtcgctaa
    29461 cggtgcacgt tttatcgcca ccaatccgga cacccacggg cgcggttttt atcccgcgtg
    29521 tggcgcgttg tgtgcaggca tcgaaaaaat ctccgggcgt aaaccgttct atgttggtaa
    29581 accgagtccg tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac
    29641 ggttattgtc ggcgataacc tgcgtaccga tattctggcg ggcttccaag caggtctgga
    29701 aacgattctg gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg
    29761 cccaagctgg atttacccgt cggtcgctga aatcgacgtt atctgaatag caaccacgtc
    29821 tacagacgtg gttggctctg atgacaaacg ccaaactgcc tgatgcgcta cgcttatcag
    29881 gcctacgcag ctcctgcaat atattgaatt ttcatgcttt tgtaggccgg ataaggcgtt
    29941 cacgccgcat ccggcatgaa caaattgcac tttgtcagca atttggcccc cgtaaaatat
    30001 aaacattcaa taaaacccgc taaaaattat cgattcatca ataaatatat cctcacacca
    30061 gacctttttt cagcaatcga caatcaccat tacgtttttt attttttcac cgccaaatcg
    30121 cttgcgcaca gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa
    30181 taccaggtta acggagaagg ttatgtgttc aatttttggc gtattcgata tcaaaacaga
    30241 cgcagttgag ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc
    30301 ggactggtcc ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat
    30361 tgttgacgtt aacgcggggg cgcaacctct ctacaaccaa caaaaaaccc acgtactggc
    30421 ggtaaacggt gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca
    30481 gttccagaca gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga
    30541 atttctcgac gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc
    30601 ctacctgatt ggtcgcgacc atctggggat catcccactg tatatgggct atgacgaaca
    30661 cggtcagctg tatgtcgcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa
    30721 agagttcccg gcggggagct atttgtggag ccaggacggc gaaatccgtt cttattatca
    30781 tcgcgactgg ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg
    30841 tcaggctctg gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct
    30901 gctttctggt ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg
    30961 tcgcgtggaa gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt
    31021 aggtctgccg ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac
    31081 ggtgcatcac gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat
    31141 ttaccacatc gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat
    31201 gtcgcgtaag atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga
    31261 agtgtttggc ggttatcttt acttccataa agcgcccaac gctaaagaac tgcatgaaga
    31321 gacggtgcgt aaactgctgg ccctgcatat gtatgactgc gcgcgcgcca acaaagcgat
    31381 gtcagcctgg ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc
    31441 gatgcgcatt aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aaaacatcct
    31501 gcgtgaatgt tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt
    31561 ctccgatggc gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt
    31621 ttctaatcag caactggaaa ctgcccgctt ccgcttcccg tacaacacgc caacctcaaa
    31681 agaagcgtat ctgtaccggg agatctttga agaactgttc ccgcttccga gcgccgctga
    31741 gtgcgtgcct ggcggtcctt ccgtcgcgtg ttcttccgct aaagcgatcg aatgggatga
    31801 agcgttcaag aaaatggacg atccatctgg tcgtgcggtt ggtgttcacc agtcggcata
    31861 taagtaagaa atgcgaaaca gccccggtga aatccggggc tgtttcagtt attgaggtgc
    31921 ttgttgattc tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa
    31981 ctcatcactg taggccagta tttcatagat taatttccca ccactattgt tgaattccga
    32041 atttcatcct gtacgacaaa agaatggctg tagggtgggg aaaaaacaca aagtaaacaa
    32101 taattgacga atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa
    32161 ttttccttga aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg
    32221 ccgataaggt atcgcgaaaa aaaagatggc tacgtagctc agttggttag agcacatcac
    32281 tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc accatctttt tttgcgggag
    32341 tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa
    32401 gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc gccaagcggt
    32461 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatctt
    32521 cttcgagtaa gcggttcacc gcccggttat tggggtatcg ccaagcggta aggcaccggt
    32581 ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatagaa gaaacaatct
    32641 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    32701 tcccgtcgta gccaccaaat tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa
    32761 tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc
    32821 gaaccctcgt accccagcca atttattcaa gacgcttaca tcgtaagtgc acccagttgg
    32881 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    32941 gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgttcatt
    33001 caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga cgctgccgcg
    33061 tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca accctaacgc
    33121 atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta acccgagatt
    33181 acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca gatccatacc
    33241 actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga ggacaggata
    33301 actggcccac gcttcaccgt aactgcgggc attcaccaga acatcaatca gggcatcgac
    33361 atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat gcgcggcatc
    33421 gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa
    33481 cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gagaaatgct tcgcgatttc
    33541 cgcctggagt tgcgccatat tcatattctg caactggcga atacgcgccg gagagtcata
    33601 ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt
    33661 aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa tcaacatgca
    33721 cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca cctgagactt
    33781 tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct tcagttccag
    33841 ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac gtaacgttac
    33901 tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt tttccaccat
    33961 atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg ccgtttccca
    34021 ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac
    34081 ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc gcacgtccgg
    34141 ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc
    34201 gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga caatggcaat
    34261 ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag
    34321 gatttttacc cccaagggct gataacgctc atactggcca caacggcagc aaagcattac
    34381 actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact
    34441 ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct
    34501 gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctgct
    34561 gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttggggcg
    34621 ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg gctgcgtggc
    34681 atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc
    34741 gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc gcagcccggt
    34801 tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac cgcgcgccga
    34861 agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt
    34921 ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc tgtttgagat
    34981 tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga acgtgaacgc
    35041 ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc tgcgtctggt
    35101 tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac
    35161 cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc tgcatctgcc
    35221 ggtacagagc ggttccgatc gcattctgaa cctgatgggc cgtactcata cggcactgga
    35281 gtacaaagcg atcatccgta aactgcgtgt ggcgcgtcca gatattcaga tcagttctga
    35341 tttcatcgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga tgaagctgat
    35401 tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc cgggtacacc
    35461 agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc tgtatattct
    35521 gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg gcaccaccca
    35581 gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg ggcgtaccga
    35641 aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat tcgtcgatgt
    35701 agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta ctgaagatga
    35761 aatgggcctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc gcaaagaaaa
    35821 cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc ggtgggcctt
    35881 gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc ctgtggcgtg
    35941 aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga taagaggaac
    36001 ggtttgaaca tagacactcg cgaaatcacc ctagagccag cagacaacgc gcgtctgttg
    36061 agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct cggcatcgag
    36121 atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg
    36181 gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca gattcaggat
    36241 atcgaaccgg aacagatcca ccttacgatt aaagaagcgc gagtactgga gcaaagtgcg
    36301 gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag
    36361 ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc
    36421 ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc
    36481 ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga agccggtgag
    36541 aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccactg
    36601 tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat tgagcgcaac
    36661 gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc
    36721 attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct gacccgtatc
    36781 ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat
    36841 actaaatcag gcttacgtca cgccatcgaa gtactggccg atgtcgaaga gatcagcttt
    36901 aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat cgttaacgcc
    36961 tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcag cgctggcggc agaacgcaaa
    37021 cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag
    37081 ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg gtgatcccac
    37141 agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa agccacagtc
    37201 tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag
    37261 tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt caggtggttg
    37321 agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc
    37381 acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca gaagaaatgg
    37441 aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga
    37501 aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga ttaacgacgg
    37561 cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag
    37621 tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca
    37681 cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga
    37741 ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg
    37801 cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct
    37861 ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg tgattagcga
    37921 agacaaagat cacattgaag ggattctgat ggcaaaagac ttgctgccgt ttatgcgcag
    37981 cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag
    38041 taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt
    38101 cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc tggaactgat
    38161 tgttggtgag attgaagacg aatatgacga agaagatgat atcgacttcc gtcagctgag
    38221 tcgtcatacc tggaccgtgc gcgcactggc ttccattgaa gacttcaacg aagcgttcgg
    38281 cacccacttt agcgatgaag aagtcgacac tatcggtggt ctggtgatgc aggcgtttgg
    38341 gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca aagtggcgat
    38401 ggccgacagt cgacgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc
    38461 gaagctggat gaataaaacc gaaactggat agataactac atggcttttg cctcattaat
    38521 tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc
    38581 cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc ttcaggcgtt
    38641 gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg gatttggcct
    38701 ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg gaatgcctgg
    38761 cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt ataccggact
    38821 gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc
    38881 cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga caggcttccc
    38941 gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg caccgataat
    39001 gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg cactggcgtt
    39061 ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc
    39121 gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg tttcgatggt
    39181 tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta atacgctgaa
    39241 gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta tctggccgga
    39301 gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac tggacggtga
    39361 gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc tcaataagca
    39421 gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac cgtacagcta
    39481 cgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccgct
    39541 ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg
    39601 tgggccatat atccagccgc cgctgtcagt aaatggtatt gagcttactg cggctatttg
    39661 ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata ccgactatct
    39721 gctgactatc tccaacgatg cgtggtttgg taaatctatt ggtccatggc aacacttcca
    39781 gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg
    39841 cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcacccg
    39901 cgaggtgtta accactaatg tgacaccgac caccggactc acaccgtatg cacgtaccgg
    39961 caactggcca ctgtgggtgc tgacggcact gtttggtttt gccgctgtgt tgatgagtct
    40021 gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg tactttctca
    40081 ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga tacaagccac
    40141 attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg tcctgtccat
    40201 ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg aaaaacgaag
    40261 gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa tattgagcag
    40321 gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt ccgggctaat
    40381 accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg cttgttgatc
    40441 ccctacgctt atacgtgaaa tcccctgact atctgcaaaa ataacctcgc cgttttcagg
    40501 tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt cttctttctg
    40561 atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa ccagtaatgt
    40621 tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg tccccgcccc
    40681 gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat caatcgctga
    40741 cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg agaggataag
    40801 gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg ccacgttaat
    40861 gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat ccgggcttaa
    40921 tgtcagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg acttttcttt
    40981 cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga aaacaacaat
    41041 gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga tcgccatatc
    41101 atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac ttaccggagc
    41161 ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt tttgccgtcg
    41221 ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga cttgtaaaac
    41281 tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa gactgcccgc
    41341 aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg cgctcagcca
    41401 acccaacgcg ccacccgcaa tcaggccgcc aatgtgacaa gcattatcta ttcctgtttg
    41461 ccgggcacca tacaacagtg tcagcgccac catacccaac agagaaaata ctcgccgctg
    41521 gtttttatgg tatgtgcccg cacctgaagc aagctgtgtt gctatcgcag cgccagctat
    41581 tcccataatt gccccggatg ccccaacgct gatgactatc tgatcactat cgcgtaacgc
    41641 ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa tcaggagtcg
    41701 ccatttcccc agaatctgtt ccgccacacc tcccacgacc aggaatgcaa acatattcat
    41761 cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc agggttcacc
    41821 cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt gatcgacctc
    41881 ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg caaaaaggat
    41941 aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag acataaggtg
    42001 tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat catcccggca
    42061 aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc ctgaaattac
    42121 aatcgtaatt aatttgcccc taagtatttt ttggcatgta agttgcttta tttactgtgt
    42181 aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag cggtagcacc
    42241 aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg caaaaacaaa
    42301 caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac aggcacaaca
    42361 ctgcacaata aggttgcaga cgataacaac acaaacactc acaacgggta tccatgcgtt
    42421 cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca cagcaatcct
    42481 cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg cagcaggcag
    42541 tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg aatcttcagt
    42601 acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg attactccaa
    42661 cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg taaaactgat
    42721 tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg attttgaatg
    42781 tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg atactatttt
    42841 cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct
    42901 gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc tcaacaaact
    42961 gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcacg gtgactcttt
    43021 ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc tgctggccgg
    43081 tgaacgtgcg aaagcgaaga aaccagacaa ctgggatatc gtcggcaagc cgcagtctca
    43141 ggaagcctac ggttgcatgt tgcgtaaaga tgatccgcag ttcaaaaagc tgatggatga
    43201 cactatcgct caggtgcaga cttccggtga agcggaaaaa tggtttgata aatggttcaa
    43261 aaatccaatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc
    43321 actgttcaaa gaaccgaatg acaaagcact gaactaatta caagaaccag gggcggaaat
    43381 tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg tcgttcccca
    43441 tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag ttaacgctgc
    43501 taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt atttttttac
    43561 aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt tttcaggtga
    43621 cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca tttttcggca
    43681 ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat gtcgaactgt
    43741 tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc ccggagctgt
    43801 tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt cagtttttcc
    43861 tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg
    43921 ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg ggcctgacgc
    43981 taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc gtcccgccga
    44041 tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact attggtctgg
    44101 tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc tgggagtcgt
    44161 ttaccgctat tacgctggcg tatgtgttga ttaacgcgat tatcatgctg gtaatgacgc
    44221 tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt acgagtttga
    44281 ctggagttcc attgtccctt ccctgccata ttagctcgac gggctggtga tcacgctgaa
    44341 aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg cggtgatgcg
    44401 cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg tattccgctc
    44461 gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt ttctgcaaaa
    44521 cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg tggcgttttc
    44581 gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa gtatttctcg
    44641 tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga tgaaactgat
    44701 tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg gcatcgtact
    44761 gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc
    44821 aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg gatttgttta
    44881 tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata
    44941 atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc
    45001 tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg ttccggcaaa
    45061 tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga aatcaccgtc
    45121 gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc ccgcgtcggg
    45181 atggtgttcc agcattttga gttgttcccc catttgtcga ttattgaaaa cctgactctg
    45241 gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc attgaaattg
    45301 ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct ttccggcggt
    45361 cagcagcagc gtgtggcaat cgctcgcgcg ttgtgtatgg atcctattgc gatgctgttc
    45421 gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga cgtgatggtg
    45481 gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg ctttgcccgc
    45541 aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga ctcgccgaaa
    45601 gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc gaaaatcctg
    45661 cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta tcgtttcgcc
    45721 cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca
    45781 acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc tatcgcaata
    45841 gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac
    45901 cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct taatcgcacc
    45961 gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac
    46021 aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac gttcgcaccg
    46081 caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc ggaacctgtc
    46141 acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag ccatccggaa
    46201 ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct tggtaactgg
    46261 acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat tgtcttccag
    46321 tcagggatcc cagtggtgat ggccggtctg gatgttactc ataaagcgca aatccacatt
    46381 gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt tgccgaactg
    46441 ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg tgcaccgctg
    46501 catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac tgttgagcgc
    46561 tgggttggcg tggaaacaca ggggaaatat acccagggta tgacggttgt tgattattat
    46621 tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg tcagggcttt
    46681 gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt gattaagatc
    46741 accacgcgtt acctgggtgg tcttaatctg tttatctctt tttctttcaa gatataaatg
    46801 caatggataa tgcagaactc accattggta tcgatctcgg cactaccaat agtttaattg
    46861 ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa tatttaacac
    46921 catccataat tagcatggat gaaaataatc atattttagt cggaaaaccg gctgtatcac
    46981 ggcgtacttc gcatccggat aaaacggcag cgttatttaa acgcgcaatg ggcagtaata
    47041 ccaactggcg gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac
    47101 gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct
    47161 ccgttccggc ttatttcagc gatgaacaac gcaagcatac ccgtttagct gcggagttag
    47221 ccgggttaaa tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcatatggcc
    47281 tgcatacgca acaaaatacc cgctcgctgg tttttgatct cggtggcggc acgtttgacg
    47341 ttacggtgct tgagtacgcc acgccggtga ttgaagttca cgcctccgct ggcgatagct
    47401 tcctcggtgg cgaagatttt acccatatgc tggtcgatga ggttttaaaa cgcgcggaag
    47461 ttgccaggac cacgcttaac gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg
    47521 caaaatgtag caatcaatcg ccattgcaca ttcgctggca gtatcaggaa gaaacgcggg
    47581 aatgcgaatt ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc
    47641 gagtgccgat tgaacaggcg ttgcgcgatg cgcgtctgaa gccgagtcaa atcgacagtc
    47701 tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt
    47761 ttggcaaatt accgtatcaa agttacgatc cgagcaccat tgtcgcgctg ggcgcagcaa
    47821 tccaggccgc ctgccgctta cgcagtgaag atattgaaga ggtaatcctc actgatattt
    47881 gcccttactc gttgggcgtt gaagttaacc gccagggcgt ttccggcatt ttctcgccga
    47941 ttattgaacg aaacaccaca gtaccggtgt cgcgtgtaga aacttactcg accatgcacc
    48001 cggagcagga ttcaattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaata
    48061 acattctggt tgaatccttc gatgtgccgt tgaagaaaac cggggcttat cagtcgattg
    48121 atattcgctt tagttatgat atcaacgggt tgcttgaagt tgacgtgctt ctgaaagacg
    48181 gcagcgttaa gtccagagtg attaaccaca gcccggtaac attgagcgcg cagcagattg
    48241 aagagagtcg gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctcatcaatc
    48301 gcacctttaa agccaaactg gaagagttgt gggcgcgagc gctgggtgac gagcgagaag
    48361 agattggccg ggtgatcacc gattttgatg cggcgctaca gtcaaacgac atggcccgcg
    48421 tggatgaagt tcggcggcgg gcgagcgatt atttagccat tgagatccca ttaacacggc
    48481 tgccggatgc ggcgtaaacg ctgcatccgg catcggcgca aaaaacacat cgtcaactaa
    48541 acaacatcgc gattttgccg agagcataac ctatcaccag caccgttaaa ctgattttga
    48601 taatgctggt aatgctctgt ggtttttcat cctcatcatt tttttcttca tggttttcag
    48661 gtgttggctc atcgacatct tcatcctccg gctcgagttt cactttcgtc aacagcatcc
    48721 gggtgataat ggcctctaat ggatgagcat ctccgccttc cagccttgta ttaagacggg
    48781 ccagcatctc ttgtttcgga aacgcttcaa gcactacctg ttccgctacc cgatacaaat
    48841 ccagtaacgg tgaatcttgc tctgtctcgc taccgactct cgatttccac cacgcccatg
    48901 ctttgcgttt gaagaataac gactcaataa acgattgacc taacggcgat aattttccgc
    48961 tatgtaaaat ctccgcccat aacagccgcg tccgggcgct ctcaccagga acatatttca
    49021 atggtttacc ttgcgcatct accatgctct gcgcttcgat aaccatcact aataacgcga
    49081 aataatctgg gcaatgacgc ttactccact gcaacaacaa attatcagct tgtgtagatg
    49141 ggtaactttc ccggtacgcg cagagatcgg caagcaggct ttccccctcg ccgcagtaca
    49201 acctttgagc acacaagtaa tatcgggcat cttcgctttc tggttcttcc atttgccact
    49261 gtttggctac aggaatgagt tccgccatac ctgtttgcac cgagctgtac cagcgcaaca
    49321 gtttgcgatg taactttgca tcatcaggaa tgaaccacgg accttccatc gccaaaaacg
    49381 cagtgacata gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tactcaacag
    49441 tctggttctg caacgcaacg ggatattcca gaaggctaag gaaatcgaaa caatcaccag
    49501 cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc cattccttcg ctgtcattaa
    49561 acgaatgcca gttcaaacgc tctgccagca aagaagcaca attcaatgaa cgatatcgcg
    49621 cttctatagc gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg
    49681 tatttaattg ctgaataaat ttctgctagg cggagggatg aaaacgatca ctttctgaat
    49741 ccagtaatgt cctgaatgca cgcagtattt catgttcagc ggcggcatct ttttcttcat
    49801 catctgcttc ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta
    49861 atgctttaac cccctgcgga tcggtttcgg gatgacataa cggtaagcga gcaagataag
    49921 cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt
    49981 tcataggtcg ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggttg
    50041 ccagggaagc gtggcataga aacgatcgct tcccgctgcc agctcttcga acatccgact
    50101 cccggcgcta tcgtatttgt acagggttag cgcctcgcgt agcgaagtgt tgtcttcgca
    50161 ctgagaagat tgccagtaga gacgccctaa cgaataggca gaaaaatagt gcatccagcc
    50221 gtgatagtaa tggtgagctc gtacatagac gcgcgattgc agccataaac tctcttcttc
    50281 cgtcagccac ttgttgagca caccgacccg tgacagaacc cccattcgaa cataatccca
    50341 ggctttgatt ccc
//
LOCUS       DAJTMK010000007        49856 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTMK010000007 DAJTMK010000000
VERSION     DAJTMK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49856
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.007"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..505
                     /locus_tag="OO135_000337"
     CDS             <1..505
                     /locus_tag="OO135_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5396304.1"
                     /translation="NYALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGL
                     KTDWRGYTVVPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAG
                     ARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPD
                     QICHISY"
     gene            553..1410
                     /locus_tag="OO135_000338"
     CDS             553..1410
                     /locus_tag="OO135_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5396305.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDIDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYP"
     gene            1441..1623
                     /locus_tag="OO135_000339"
     CDS             1441..1623
                     /locus_tag="OO135_000339"
                     /inference="COORDINATES: protein motif:HMM:NF012635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5396306.1"
                     /translation="MVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVSTTGVPP
                     ETGRFEATATVRINFD"
     gene            1635..2177
                     /locus_tag="OO135_000340"
     CDS             1635..2177
                     /locus_tag="OO135_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5396307.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVIYFDLVYQ"
     gene            2185..2700
                     /locus_tag="OO135_000341"
     CDS             2185..2700
                     /locus_tag="OO135_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5396308.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            2666..3403
                     /locus_tag="OO135_000342"
     CDS             2666..3403
                     /locus_tag="OO135_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5396309.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            3514..4524
                     /gene="pyrD"
                     /locus_tag="OO135_000343"
     CDS             3514..4524
                     /gene="pyrD"
                     /locus_tag="OO135_000343"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5396310.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKDKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            4698..5240
                     /gene="zapC"
                     /locus_tag="OO135_000344"
     CDS             4698..5240
                     /gene="zapC"
                     /locus_tag="OO135_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5396311.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SSAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(5237..6346)
                     /gene="ycbX"
                     /locus_tag="OO135_000345"
     CDS             complement(5237..6346)
                     /gene="ycbX"
                     /locus_tag="OO135_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5396312.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            6590..8698
                     /gene="rlmKL"
                     /locus_tag="OO135_000346"
     CDS             6590..8698
                     /gene="rlmKL"
                     /locus_tag="OO135_000346"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5396313.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSRGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            8710..10617
                     /locus_tag="OO135_000347"
     CDS             8710..10617
                     /locus_tag="OO135_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5396314.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVGEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPYKTVMDNIAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQLAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            10747..12000
                     /gene="pqiA"
                     /locus_tag="OO135_000348"
     CDS             10747..12000
                     /gene="pqiA"
                     /locus_tag="OO135_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5396315.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            12005..13645
                     /gene="pqiB"
                     /locus_tag="OO135_000349"
     CDS             12005..13645
                     /gene="pqiB"
                     /locus_tag="OO135_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5396316.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            13642..14205
                     /gene="pqiC"
                     /locus_tag="OO135_000350"
     CDS             13642..14205
                     /gene="pqiC"
                     /locus_tag="OO135_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5396317.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNILVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            14461..14628
                     /gene="rmf"
                     /locus_tag="OO135_000351"
     CDS             14461..14628
                     /gene="rmf"
                     /locus_tag="OO135_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5396318.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(14698..15216)
                     /gene="fabA"
                     /locus_tag="OO135_000352"
     CDS             complement(14698..15216)
                     /gene="fabA"
                     /locus_tag="OO135_000352"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5396319.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(15285..17045)
                     /locus_tag="OO135_000353"
     CDS             complement(15285..17045)
                     /locus_tag="OO135_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5396320.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISSVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            17231..17683
                     /gene="matP"
                     /locus_tag="OO135_000354"
     CDS             17231..17683
                     /gene="matP"
                     /locus_tag="OO135_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5396321.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(17759..18799)
                     /gene="ompA"
                     /locus_tag="OO135_000355"
     CDS             complement(17759..18799)
                     /gene="ompA"
                     /locus_tag="OO135_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5396322.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDGLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(19156..19665)
                     /gene="sulA"
                     /locus_tag="OO135_000356"
     CDS             complement(19156..19665)
                     /gene="sulA"
                     /locus_tag="OO135_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5396323.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            19884..20513
                     /gene="sxy"
                     /locus_tag="OO135_000357"
     CDS             19884..20513
                     /gene="sxy"
                     /locus_tag="OO135_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5396324.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(20476..22638)
                     /gene="yccS"
                     /locus_tag="OO135_000358"
     CDS             complement(20476..22638)
                     /gene="yccS"
                     /locus_tag="OO135_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5396325.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(22648..23094)
                     /locus_tag="OO135_000359"
     CDS             complement(22648..23094)
                     /locus_tag="OO135_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5396326.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            23217..25271
                     /gene="helD"
                     /locus_tag="OO135_000360"
     CDS             23217..25271
                     /gene="helD"
                     /locus_tag="OO135_000360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5396327.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNKLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(25303..25761)
                     /gene="mgsA"
                     /locus_tag="OO135_000361"
     CDS             complement(25303..25761)
                     /gene="mgsA"
                     /locus_tag="OO135_000361"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5396328.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(25857..26519)
                     /locus_tag="OO135_000362"
     CDS             complement(25857..26519)
                     /locus_tag="OO135_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5396329.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSKLDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            26692..27105
                     /locus_tag="OO135_000363"
     CDS             26692..27105
                     /locus_tag="OO135_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5396330.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSESAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(27150..27467)
                     /gene="hspQ"
                     /locus_tag="OO135_000364"
     CDS             complement(27150..27467)
                     /gene="hspQ"
                     /locus_tag="OO135_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5396331.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(27525..28715)
                     /gene="rlmI"
                     /locus_tag="OO135_000365"
     CDS             complement(27525..28715)
                     /gene="rlmI"
                     /locus_tag="OO135_000365"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5396332.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            28810..29088
                     /gene="yccX"
                     /locus_tag="OO135_000366"
     CDS             28810..29088
                     /gene="yccX"
                     /locus_tag="OO135_000366"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5396333.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(29085..29414)
                     /gene="tusE"
                     /locus_tag="OO135_000367"
     CDS             complement(29085..29414)
                     /gene="tusE"
                     /locus_tag="OO135_000367"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5396334.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(29505..30164)
                     /gene="yccA"
                     /locus_tag="OO135_000368"
     CDS             complement(29505..30164)
                     /gene="yccA"
                     /locus_tag="OO135_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5396335.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQQPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(30371..30458)
                     /locus_tag="OO135_000369"
     tRNA            complement(30371..30458)
                     /locus_tag="OO135_000369"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30422..30424),aa:Ser,seq:tga)
     gene            30885..32003
                     /gene="hyaA"
                     /locus_tag="OO135_000370"
     CDS             30885..32003
                     /gene="hyaA"
                     /locus_tag="OO135_000370"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5396336.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            32000..33793
                     /gene="hyaB"
                     /locus_tag="OO135_000371"
     CDS             32000..33793
                     /gene="hyaB"
                     /locus_tag="OO135_000371"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5396337.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            33812..34519
                     /gene="hyaC"
                     /locus_tag="OO135_000372"
     CDS             33812..34519
                     /gene="hyaC"
                     /locus_tag="OO135_000372"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5396338.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            34516..35103
                     /locus_tag="OO135_000373"
     CDS             34516..35103
                     /locus_tag="OO135_000373"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5396339.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPADISAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            35100..35498
                     /gene="hyaE"
                     /locus_tag="OO135_000374"
     CDS             35100..35498
                     /gene="hyaE"
                     /locus_tag="OO135_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5396340.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            35495..36352
                     /locus_tag="OO135_000375"
     CDS             35495..36352
                     /locus_tag="OO135_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5396341.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQD
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            36486..38030
                     /gene="appC"
                     /locus_tag="OO135_000376"
     CDS             36486..38030
                     /gene="appC"
                     /locus_tag="OO135_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5396342.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHLLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            38042..39178
                     /gene="appB"
                     /locus_tag="OO135_000377"
     CDS             38042..39178
                     /gene="appB"
                     /locus_tag="OO135_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5396343.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            39191..39283
                     /gene="cbdX"
                     /locus_tag="OO135_000378"
     CDS             39191..39283
                     /gene="cbdX"
                     /locus_tag="OO135_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5396344.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            39363..40661
                     /gene="appA"
                     /locus_tag="OO135_000379"
     CDS             39363..40661
                     /gene="appA"
                     /locus_tag="OO135_000379"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5396345.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPSGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(40776..42956)
                     /gene="etk"
                     /locus_tag="OO135_000380"
     CDS             complement(40776..42956)
                     /gene="etk"
                     /locus_tag="OO135_000380"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5396346.1"
                     /translation="MTTKNMNTPPSSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(42976..43422)
                     /gene="etp"
                     /locus_tag="OO135_000381"
                     /gene_synonym="yccY"
     CDS             complement(42976..43422)
                     /gene="etp"
                     /locus_tag="OO135_000381"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5396347.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(43410..44549)
                     /locus_tag="OO135_000382"
     CDS             complement(43410..44549)
                     /locus_tag="OO135_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5396348.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLCQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(44595..46691)
                     /locus_tag="OO135_000383"
     CDS             complement(44595..46691)
                     /locus_tag="OO135_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5396349.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPLDLMTIGPNRNRTVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(46691..47437)
                     /locus_tag="OO135_000384"
     CDS             complement(46691..47437)
                     /locus_tag="OO135_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5396350.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPSALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(47434..48078)
                     /locus_tag="OO135_000385"
     CDS             complement(47434..48078)
                     /locus_tag="OO135_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5396351.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDSSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(48185..48490)
                     /locus_tag="OO135_000386"
     CDS             complement(48185..48490)
                     /locus_tag="OO135_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR5396352.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(48932..49144)
                     /gene="cspH"
                     /locus_tag="OO135_000387"
     CDS             complement(48932..49144)
                     /gene="cspH"
                     /locus_tag="OO135_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5396353.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            49430..49642
                     /gene="cspG"
                     /locus_tag="OO135_000388"
     CDS             49430..49642
                     /gene="cspG"
                     /locus_tag="OO135_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5396354.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            49653..49841
                     /locus_tag="OO135_000389"
     CDS             49653..49841
                     /locus_tag="OO135_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5396355.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
BASE COUNT        12303 a        12303 c        13114 g        12136 t
ORIGIN      
        1 gaactacgct ctttcaggca gtttagttgc ccattcacag ggcattaccc tggggcaatc
       61 gctgggggaa actaacgtgc tgattgcagc accaggcgca gagaatactc gtgtggcgaa
      121 cagcaccggg ctgaaaactg actggcgcgg atataccgtt gtcccctatg ccacttctta
      181 tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa cgtaacgtgg atcttgaaaa
      241 tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt ctggcggagt tcaatgccca
      301 tgcgggagca agggtattaa tgaaaacatc aaagcagggt ataccgctgc gttttggcgc
      361 gatagcgacg ctggacggca tacagactaa tagcggcatt attgatgatg atggctcgct
      421 ctatatgtcc ggtttgcccg ctcagggagc gataactgta cgctggggcg aagctcccga
      481 tcaaatttgt catatcagtt actagcttac cgaacaacaa attaactctg cgattacgcg
      541 aatggatgcc atatgcagat aatctttgga gaaaaatgcg tgttattact acgactattt
      601 tttgccgccg tcttaatgct atggtgcgct caaactgctg cttatagcgg gcagtgtcat
      661 accactcagg ggaatccgta tattggcgtc aattttggcg ttaaaaccct ggaggaagaa
      721 gaaaatacgg ctggagtagt taaagacaaa ttttatcagt ggaacgaatc gaatgattat
      781 tatgtttcct gtgattgcga tatagacaat gtcagaagtg gccgatgggc attcgccgcg
      841 gattcaccgt tagtctattt aggcgacaac tggtacaaaa ttaatgacta tcttgccgcc
      901 aaagttttat tgcaggttaa aggcagttct cctactgcgg ttcctttcga aaacgtgggc
      961 acaggggcag atacacgatg gcatatttgc gatcccggcg gtcaacgttt aggtggccag
     1021 ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat tgcagccgtt cgttggttcg
     1081 gtcgtcattc ctcctatggc gctggcgcga ttatttgaat gctacaacat acccgcaggt
     1141 gattcctgca cgactacagg tacaccggtt ttagtgtatt acctgtctgg tactatcaat
     1201 tcacttggct catgttccgt caatgccgga gaaacaatcg aggtcgatct gggcgacgta
     1261 tttgcggcta actttcgtgt tgtagggcat aagcctcttg gggccagaac ggcagaactt
     1321 gcaattccag tcaggtgtaa cacgggaaac gcggggttag ttaacgtcaa cctgagtctg
     1381 acggcaacta cagaccccag ctatccctag gcgattaaga cgtcacgtcc tggcgtgggc
     1441 gtggtggtga ccgatagcca gaacaacatt atttcccctg ctggtggaac attaccgctc
     1501 tctattcctg atgatgcaga cagtatcgcg cgaatgaatg tctatccagt cagcacgaca
     1561 ggtgtaccac cagaaaccgg gcgatttgaa gccacggcaa cggtgagaat aaattttgat
     1621 taacgcgtga acgtatgaag aaaaaaacga tatttcagtg cgttatcttg ttctttagca
     1681 ttcttaacat ccatgtcggg atggctgggc ctgaacaagt tagtatgcat atttatggga
     1741 atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc attacaaaat attcatattg
     1801 gtgattttaa tatcagtgat tttcaggctg cgaataccgt aagcactgct gctgatttga
     1861 atatcgatat caccggttgt gccgctggta ttactggcgc ggacgtcctt tttagcggcg
     1921 aggctgacac ccttgcgccg acactgctca aactaactga cacaggcgga agcggtggta
     1981 tggcaacggg gattgccgtg caaattcttg atgcgcaaag tcagcaagaa atcccgctca
     2041 atcaggtcca gcctcttacg cccttaaaag ccggggataa cacactcaaa tatcaacttc
     2101 gttataagtc cacaaaggcg ggagcaacgg gcggtaatgc gacggcggtt atctattttg
     2161 atctggttta ccagtgaagg agtgatgttg aaacggataa tctggattct gttcttactg
     2221 ggattaacgg ggggctgtga gctatttgcc catgatggca cggtcaacat tagcggatcg
     2281 tttcgccgta atacatgcgt gctggcacag gatagcaagc aaatcaatgt gcagttaggc
     2341 gatgtctcgc tgacacgttt ttttcatggg aattatggcc cggagaaatc tttcatcatt
     2401 aatttgcagg attgcggaac agatgtcagc acggttgacg tcaccttttc aggaaccccc
     2461 gacggagtgc agagcgaaat gctgtccatt gaaagtggta cggatgcagc cagcgggctg
     2521 gcaattgcga ttctggatga tgcgaagata ttgattccgc ttaaccaggc gagtaaagac
     2581 tacagcctac atagcggtaa ggtgccatta actttttatg cgcaattgcg acctgtcaat
     2641 agcgatgtgc agtcgggtaa agtgaatgcc agcgcgacat ttgtacttca ttatgactaa
     2701 tacgtggaac agattggcgc tcttgatttt cgccgtttta tcgctgctgg tggcgggtga
     2761 attgcgggca ggagtggtgg tcggcggaac gcgatttatc tttccggcag acagagaatc
     2821 gatatctatt ttactgacta ataccagtca ggaatcctgg ctgattaaca gtaaaatcaa
     2881 ccgcccaacg cgttgggccg ggggggaagc gtcgacagtg ccagcaccat tactggccgc
     2941 tccgccactt attctcctga agcccggtac tacaggcacg ttgcgcttgc tgagaacgga
     3001 aagcgacatc ttgcctgtgg atcgcgaaac gctatttgag ttaagcattg ccagcgtgcc
     3061 atccggcaaa gttgaaaatc agagcgtaaa agtggcgatg cgctcggtat ttaaactgtt
     3121 ctggcgaccc gaaggtttgc cgggcgaccc gctggaagct tatcaacaat tacgctggac
     3181 acggaattcg cagggtgtac aactcactaa cccaacgcct tactacatta acctgattca
     3241 ggtgagcgta aatggtaaag cgttaagtaa tgcgggagtt gtgccgccta aaagccagcg
     3301 tcagacaagc tggtgtcagg caatcgcacc gtgtcatgtc gcctggcggg cgataaatga
     3361 ttacgacggg ttgagtgcaa agaaggagca aaatctgccc tgaaacaggt tcggaaaacg
     3421 tttgcgtttt ttttgccgca ggtcaattcc cttttggtcc gaactcgcac ataatacgcc
     3481 cccggtttgc acaccgggaa tccaggagag ttcatgtact accccttcgt tcgtaaagcc
     3541 cttttccagc tcgatccaga gcgcgctcat gagtttactt ttcagcaatt acgccgtatt
     3601 acaggaacgc cgtttgaagc actggtgcgg cagaaagtgc ctgcgaaacc tgttaactgc
     3661 atgggcctga cgtttaaaaa tccgcttggt ctggcagccg gtcttgataa agacggggag
     3721 tgcattgacg cgttaggcgc gatgggattt ggatcgatcg agatcggtac cgtcacgcca
     3781 cgtccacagc caggtaatga caagccgcgt ctctttcgtc tggtagatgc cgaaggtttg
     3841 atcaaccgta tgggctttaa taatcttggc gttgataacc tcgtagagaa cgtaaaaaag
     3901 gcccattatg acggcgtcct gggtattaac atcggcaaaa ataaagatac gccagtggag
     3961 cagggcaaag atgactatct gatttgtatg gaaaaaatct atgcctatgc gggatatatc
     4021 gccatcaata tttcatcgcc gaatacccca ggattacgca cgctgcaata tggtgaagcg
     4081 ctggatgatc tcttaaccgc gattaaagat aagcaaaatg atttgcaagc gatgcaccat
     4141 aaatatgtgc cgatcgcagt gaagatcgcg ccggatcttt ctgaagaaga attgatccag
     4201 gttgccgata gtttagttcg ccataatatt gatggcgtta ttgcaaccaa taccacactc
     4261 gatcgttctc ttgttcaggg aatgaaaaat tgcgatcaaa ccggtggctt aagtggtcgt
     4321 ccgcttcagt taaaaagcac cgaaattatt cgccgcttgt cactggaatt aaacggtcgc
     4381 ttaccgatca tcggtgttgg cggcatcgac tcggttatcg ctgcgcgtga aaagattgct
     4441 gcgggtgcct cactggtgca aatttattct ggttttattt ttaaaggtcc gccgctgatt
     4501 aaagaaatcg ttacccatat ctaattattt cttcgaattc ctctttataa gataatcagg
     4561 gctttatttt cagccctggt tgttttatat tcatcgctgt tgcttattta gacattttgt
     4621 acttttattg ttgaggttat taagcgaagc gacaatggat tgtgttgctg cggttttata
     4681 gggtagggga gaggcagatg cgaattaaac cagacgataa ctggcgttgg tattacgatg
     4741 aagagcacga tcgaatgatg ctcgatttag ccaatggtat gctatttcgc tcacgttttg
     4801 cgcgcaagat gttgactcca gatgcctttt cttccgcagg cttttgcgtt gacgacgccg
     4861 cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa tttatctaaa gaacaaaaag
     4921 ccgaactggt gctgaatgca ctggtagcaa ttcgctatct caaaccgcaa atgccgaaaa
     4981 gctggcattt tgtttcccat ggtgaaatgt gggtgcccat gccgggcgat gcggcctgcg
     5041 tttggttgag cgatactcac gagcaggtca atttgctggt cgttgaatct ggcgaaaatg
     5101 ccgcactatg cctgctggca caaccctgcg ttgttattgc gggtcgcgcg atgcagttgg
     5161 gcgacgccat taaaatcatg aacgacaggc tgaaaccgca ggttaatgtt gacagcttca
     5221 gcctcgaaca ggcagtctaa cgcgccaact taagtgcagt cttcggtaca cagctacagc
     5281 aaagaatggt gccatcatcg cccattgctg attttttcag cggcgagact tcgccttcta
     5341 aaatctgaac acggcaactt ccacaaatgc ccgcgcggca agaataaggg atacgaattc
     5401 cctgattttc taattgctcc agcaacacct gttggttatt tccacgaaat gcctgtccct
     5461 gccagtcaat atctacattt gcgtccggtt gttgcgtgat gttggcggta tcatcagcgg
     5521 cagctgcgcc gtaaattttt gccggagccg ttgccagaat ttccacctca tcgccaacgc
     5581 gaatcacgcc gctattacgg gcaattaaat tctgaccaaa atcgacatcg ccgttatcct
     5641 gggcagtgcg gaaagattgc aatgttttta atggttcgcc tgccggatgt ttttgccctt
     5701 tttctgggct gacggtggtg aaaatacagc ggctacaagg tttaaccaca tcaaacacca
     5761 catcaccaat gcgaatcact ttccagcgat cttcttccca ggctgacgcg ccggaaacca
     5821 ccagattggg gcggaattgc tccattttta cactggccgg acaacgttgt tggagatcac
     5881 gtaacgaggc ttcgttagca agaaggtaag gatagccatc agcaaatgac agaggtacag
     5941 tgttgtggcg tttcacgcgc cgggtcattt gtggccccac ccagcgtaat tgcacttcgc
     6001 gggagaaaaa tccacttagc catttgttga tcgcgtctgg cgcaattcgc gcggtaaaat
     6061 gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa atcagcaaaa cgaacatatg
     6121 cgctactgcc atctggtgcg gtgagatgca agccatcatg cacaggtgaa ggagtaaacc
     6181 gtaccatctg gggaaactgg cgagcggtaa taaacgtacc gtcaggttcc gtgatcataa
     6241 agatgcgatc gaaggccaga ccactgacat cagccagagc atgtgtaaga ccaatgccgc
     6301 gcatcgattt aacaggatga ataaaaagcc gggttaatgt cgccacggtg cggtcctcaa
     6361 atgaaaataa gccctcaact ttatgacatg acgcgcttat tagctataat gcgcaacaat
     6421 tttcttagcg cctgtcccat taggctattt tatttgtcag tttggccctg ggttgtgctc
     6481 gaaatcctca cgtacttgtg tacgctccgg tttctccgcg ctgtccatgt ccagactgac
     6541 tgcaacaatt acgcctactg cggtaggctc ttagagtaaa agtgacgata tgaattctct
     6601 gtttgccagt acggcccgtg ggctggaaga gctgttaaaa actgaactgg aaaacctggg
     6661 ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc aagggcgaca cacggcttgt
     6721 ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt atgttgccgc tgggcgagtg
     6781 taaggtttac agcgatttag acctctatct cggtgttcag gcgatcaact ggacagagat
     6841 gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt ttgaatgaca ccatccgcaa
     6901 cagtcagtac ggcgcgatga aagtgaaaga cgcgatcgtc gatgctttca cgcggaaaaa
     6961 tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc cgcgttaacg tctggctgca
     7021 taaagaaacc gccagtatcg ctcttgatct gagtggtgat ggtttacatc tgcgtggcta
     7081 tcgcgatcgt gctggtattg cgccgatcaa agaaaccctg gcagccgcta ttgtgatgcg
     7141 atccggctgg cagcctggaa caccgctgct cgatccgatg tgtggttccg gtacgttgct
     7201 gattgaagcg gcgatgctgg cgaccgatcg cgcacccggc ttgcaccgtg ggcgttgggg
     7261 ctttagcggc tgggcgcagc atgatgaagc tatctggcag gaagtgaaag cggaagcgca
     7321 aactcgcgcc cgtaaaggcc tggctgagta tagctctcat ttttacggtt cggacagcga
     7381 cgcacgggtg attcaacgtg cacgcactaa cgcccgtctt gcggggattg gtgaactgat
     7441 cacctttgag gtgaaagatg tcgcgcaact gaccaatccg ctgccgaaag ggccgtacgg
     7501 cacagtgttg agcaacccgc catacgggga acgtctggac agcgaaccgg cgctgattgc
     7561 gctgcatagc ctgctgggtc ggatcatgaa aaaccagttt ggtggctgga atctctcttt
     7621 gtttagtgcc tcgccggatc tgctaagctg cttgcagctg cgtgcagaca aacagtacaa
     7681 ggcgaaaaac ggcccgctgg actgcgtaca gaaaaattac cacgttgccg aaagcactcc
     7741 agacagcaaa ccggcgatgg tagcggaaga ctacgccaac cgcctgcgta agaacctcaa
     7801 aaaattcgag aagtgggctc gccaggaagg aattgaatgt taccgcctgt atgacgccga
     7861 tctgccggaa tataacgttg ccgttgaccg ttattccgac tgggtggtgg tgcaggagta
     7921 tgcaccgcca aaaactattg atgctcacaa agcgcgtcag cgtctgttcg atattatcgc
     7981 tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg gtgctgaaaa cccgtgaacg
     8041 ccagaagggc aaaaaccagt accagaaact gggcgagaag ggcgagtttc ttgaagttac
     8101 cgaatataac gctcacttgt gggtgaacct gacggattat ctcgatactg gtctgttcct
     8161 cgatcaccgt atcgcccgcc gtatgctcgg tcagatgagc agaggcaaag atttcctcaa
     8221 cctgttctct tataccggta gcgccaccgt gcatgcggga ttaggcggcg cgcgcagcac
     8281 caccaccgtg gatatgtcgc gtacttatct ggagtgggca gaacgcaacc tgcgtctgaa
     8341 tggcctaacc gggcgtgcgc atcgcctgat tcaggccgat tgcctggcgt ggctgcgtga
     8401 ggcaaatgaa cagtttgatc tgatctttat cgatccgcca accttctcta actcaaaacg
     8461 aatggaagat gcgtttgatg ttcagcgcga tcatctggcg ctgatgaaag atttgaaacg
     8521 tctgctgcgt gcaggtggga cgatcatgtt ctcgaacaac aaacgtggct tccgtatgga
     8581 tctcgacggc ctggcaaaac tgggactgaa agcacaagaa attacgcaaa aaacgctctc
     8641 ccaggatttc gcccgtaacc gccagatcca caactgctgg ctgattaccg cagcctgaaa
     8701 ggaatagtaa tgtcattaat cagtatgcat ggcgcatggc tgtcgttcag cgacgcgccg
     8761 cttctcgata acgcagaact gcatatcgaa gataacgaac gtgtttgtct ggtgggccgc
     8821 aacggcgcgg gcaaatcgac gttaatgaaa atcctcaacc gtgaacaagg gctggatgac
     8881 ggtcgcatta tttacgagca agatttgatt gtagctcgtc tgcaacagga tccgccgcgt
     8941 aacgttgagg gtagcgttta tgatttcgtt gccgaaggca ttgaagaaca agcggaatat
     9001 ctgaaacgct atcacgatat ttcgcgcctg gtgatgaacg acccgagcga gaaaaatctc
     9061 aacgaactgg cgaaggttca ggaacagttg gatcaccaca acctgtggca gctggaaaac
     9121 cgcatcaacg aagtgctggc gcaactgggg ttagatccta atgttgcgct gtcgtcgctt
     9181 tccggcggct ggttgcgtaa agcggcatta ggacgcgcgc tggtgagtaa tccgcgcgtg
     9241 ctgttgcttg atgaaccgac aaaccacctg gatattgaaa ccatcgactg gctggaaggg
     9301 ttccttaaaa ccttcaatgg gacgattatt ttcatctccc acgaccgttc gtttatccgc
     9361 aatatggcga cgcgcattgt tgatctcgat cgcggcaagc tggtgaccta tccagggaat
     9421 tacgaccagt acctgcttga aaaagaagaa gccctgcgcg tggaagaact acaaaatgcc
     9481 gagttcgatc gcaaactggc gcaggaagag gtgtggatcc gccaggggat caaagcacgc
     9541 cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcaa tgcgtcgcga gcgtggtgaa
     9601 cgtcgcgaag tgatgggtac cgcaaagatg caggtgggag aggccagccg ctccggtaag
     9661 atcgttttcg aaatggaaga cgtttgctac caggttgacg gtaagcaact ggtgaaagat
     9721 ttttcggccc aggttctacg tggcgacaaa attgtcctga ttggtccgaa tgggtgcggc
     9781 aaaaccacgc tgctgaaact gatgctcggt cagcttcaag cggacagcgg gcgtattcac
     9841 gttggcacca aactggaagt ggcttatttc gatcagcacc gcgcggaact ggatccctat
     9901 aaaacggtga tggataacat tgccgaaggt aagcaagagg tgatggttaa cggcaagcca
     9961 cgccacgtat tgggctattt gcaggacttt ctgttccatc cgaaacgggc gatgacgccg
    10021 gtacgtgcgc tttctggcgg tgagcggaac cgcttgctgc tggcgcgttt gttcctcaaa
    10081 ccaagcaact tattgattct tgacgaaccg accaacgatc ttgatgtcga aacgctggaa
    10141 ctgctggaag aactgatcga cagctatcag ggcacggtat tgctggttag ccacgatcgt
    10201 cagtttgtcg ataacaccgt tacagaatgt tggatcttcg aaggcggcgg taaaattggt
    10261 cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag agcagtatgt ggcgctcaaa
    10321 cagcttgcgg tgaaaaaaac cgaagaagcc gccgcggcaa aagcagaaac tgtaaaacgc
    10381 agcagtagca aactaagcta taaattgcag cgcgaactgg agcagctacc gcaattgctc
    10441 gaagatctgg aggcgaagct ggaagcccta cagacgcagg tggcggatgc ttccttcttc
    10501 agtcagccac atgagcagac acaaaaagtg ctcgccgata tggctgctgc agagcaggag
    10561 ctggagcaag cctttgaacg ctgggagtat cttgaagcgt taaaaaatgg tggctgatcg
    10621 caaaatagtc gatttaaaca gagacagctg acaacggtaa aattgcctga tgaactgggt
    10681 ttatcaggca tgaaaaccaa cactttgtta gtcatctgta aaatagcgca tcattaagga
    10741 gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc tgtgctcgca gtgtgacatg
    10801 ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg catgtccccg gtgtggcaca
    10861 acgttaaccg tggcgtggga tgcccctcgg cagcgtccga ccgcctatgc gttggctgca
    10921 ctgttcatgc tgttgctgtc caacttgttt ccttttgtga atatgaacgt tgcaggagtt
    10981 accagtgaaa ttacattact ggaaattccc ggcgtgcttt tttctgagga ctacgccagc
    11041 ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg cgttttgtct gataaccatt
    11101 ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa aagagcaact ggcacgggtg
    11161 ctttttcaac tcaaaacctg gggaatggcg gagattttcc tcgcgggagt gctggtcagt
    11221 ttcgttaaac tgatggctta cggcagcatt ggggtaggca gcagctttct cccctggtgt
    11281 ttattttgtg tcctgcaact gcgcgctttt cagtgcgttg atcgtcgctg gttatgggac
    11341 gacatcgccc cgatgccaga actgcgccag ccgctaaaac caggcgtcac ggggatacgt
    11401 caggggctgc gttcgtgctc ctgttgtacg gcaatccttc ctgctgatga acccgtgtgc
    11461 ccgcgttgta gtaccaaagg gtacgttcgg cgtagaaaca gcctgcagtg gacactcgcg
    11521 ctgcttgtaa cgtccatcat gctgtatctt ccggctaata ttttgcccat catggtgacg
    11581 gatttattag gctcgaagat gccgtcgacg attctcgctg gggtcattct gttatggagc
    11641 gaaggctctt atcccgtcgc tgcggtgatc tttctggcca gtattatggt gccaacgtta
    11701 aagatgatcg ccatcgcgtg gctgtgttgg gatgccaaag ggcatggcaa gcgcgacagt
    11761 gaaagaatgc atttgattta tgaagttgtt gagtttgtag gccgctggtc gatgattgac
    11821 gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg gaggtttaat gagtatttat
    11881 ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa tgacaatgtt ttctgctatg
    11941 acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat cagagcatga ggagtcctga
    12001 cagtatggaa tctaataatg gggaagccaa aatccagaaa gtgaagaact ggtctcccgt
    12061 gtggatattt cctatcgtca cggcgctcat tggagcctgg gttctatttt atcattacag
    12121 ccatcaggga ccggaagtga ccctgatcac cgcgaatgcg gaaggaattg aaggtggcaa
    12181 aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt gaaagcgcca cactggctga
    12241 tgatttgacg cacgttgaaa tcaaagcgcg gctgaattcc ggtatggaaa aattgctgca
    12301 taaagacacc gtcttttggg tggtgaaacc gcagattggt cgcgaaggga ttagcggcct
    12361 gggaacgctg ctgtctggag tttatatcga actgcagcca ggcgcgaaag gcagcaaaat
    12421 ggataaatac gatttgctgg actcgccacc gttggccccg cctgatgcga aaggtatccg
    12481 tgtgattctc gatagcaaaa aagccgggca gctctcgcca ggagatccgg tgctgttccg
    12541 tggctatcgg gtaggttcgg ttgaaaccag caccttcgat acgcaaaaac gcaatatcag
    12601 ttatcaactg ttcatcaatg caccttatga ccgactggtg accagcaatg ttcgcttctg
    12661 gaaagatagt ggcattgcgg ttgatctgac gtcagcgggg atgcgtgtgg agatgggctc
    12721 attgacaacg ctgcttagtg gcggtgttag ctttgatgtg ccggaaggtc tggatttagg
    12781 gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat gatcagaaga gcattcagga
    12841 ttcgttgtac accgatcaca ttgattatct gatgttcttt aaagattcgg tacgcggtct
    12901 gcaaccggga gctccggtag aattccgggg tattcgcctg ggtaccgtaa gcaaagtgcc
    12961 attctttgcg ccgaatatgc gtcagacatt taacgatgat taccgtattc cggtactgat
    13021 tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa aatacggatg ttgttgagca
    13081 ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg aaaaccggaa acctggtcac
    13141 tggtgcactg tatgttgatc tcgatttcta tccaaatacg cctgcaataa ccggtattcg
    13201 tgaatttaat ggttatcaga ttatcccgac tgttagcggc ggcctggcgc aaatccagca
    13261 acgactgatg gaagcgttgg ataagatcaa caaactgcca ttgaatccga tgattgaaca
    13321 ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa aacctgcaaa cgacgctgga
    13381 tagcatgaac aagatcctcg ctagccagtc gatgcagcag ttgccgacgg atatgcagtc
    13441 aacgttgcgt gaattgaatc gcagcatgca gggcttccag cctggctccg cagcctacaa
    13501 caagatggtg gcggatatgc agcgccttga tcaggtgttg cgagaactgc aaccggtgct
    13561 gaaaacgctc aatgagaaga gtaacgcgct ggtatttgaa gcgaaggaca aaaaagatcc
    13621 agagccgaag agggcgaaac aatgaaaaag tggctagtga cgattgcagc actgtggctg
    13681 gccggatgca gctccggcga aattaataaa aactattacc agttacctgt ggtgcagagc
    13741 ggtacacaaa gtaccgccag ccagggcaat cgtctgttat gggtagagca ggtcactgtt
    13801 cctgactatc tggcggggaa tggtgtggtt tatcaaacca gtgatgtgaa gtatgtgatt
    13861 gccaacaaca acttgtgggc cagcccgttg gatcaacagt tgcgcaacat cctggttgcc
    13921 aacttgagta cgcaactgcc cggctgggtg gttgcctccc agcctctggg aagcgtccag
    13981 gacacgctca atgttaccgt aacggagttt aacggtcgct atgatggcaa ggtcattgtc
    14041 agtggtgagt ggctgttgaa ccaccaggga caactgatca aacgtccgtt ccgtctggaa
    14101 ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag tgctggccgg tgtctggagt
    14161 caggaagccg cttctattgc acaagagata aagcgtctac cttaattata aagatttgta
    14221 agtataaccg tctccggtat gttgcctgag gcggtttttt tgtttctaac gtgcggaaaa
    14281 atttgttcct cttcacattt tttgtacaac cgacatgccc gtgtagctca caaatatgac
    14341 agtggcgtga attttgcgca ttgacggcag ttatgattcg cggtattgct taactgtgat
    14401 tgcacattta gtaatcactg ttttcttttc caccagaaac cagtatgagg gaaacgaggc
    14461 atgaagagac aaaaacgaga tcgcctggaa cgggcacatc aacgtggtta tcaggccggc
    14521 atcgccggac gctcaaaaga aatgtgtccc tatcagacgc tgaatcaaag gtcacaatgg
    14581 ctgggaggct ggcgagaagc catggcggac agggtagtaa tggcctgatt ctgtctcttt
    14641 aaaaagaaac ctccgcattg cggaggtttc gccttttgat actctgtctg atgataatca
    14701 gaaggcagac gtatcctgga acagaccgac tttcaggtcg ctggcggtat agatcagacg
    14761 accatcaacc agcacttcgc catctgccag acccataatc agacgacgat taacaatgcg
    14821 tttaaagtga atacggtagg tcactttttt cgctgtcggc agtacctgac cagtgaattt
    14881 cacttcgcca acgcccagcg cgcggccttt accttcgccg cccagccagc cgaggtagaa
    14941 ccctactagc tgccacattg cgtccaggcc caggcatccc ggcataaccg gatcgccaat
    15001 gaagtggcat ccgaagaacc acagatccgg attgatatcc agttctgctt caacatagcc
    15061 tttgtcgaag ttaccacccg tttcggtcat tttgaccaca cggtccatca tcagcatgtt
    15121 cggtgctggc aattgcgggc ctttagcgcc aaacagttca ccgcgaccag aggcaagaag
    15181 gtcttctttt gtataggatt cgcgtttatc taccatgttc tctgtaagcc ttattttatt
    15241 gaagcacgca ggatagctaa cacgtgtacg ctgaacaagt ccgatcagtt cggaataaac
    15301 cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc cttcctgttg cgatgcttgc
    15361 gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc cttcgccatc ccacaccaga
    15421 tttaataata acggcagtgc gtcagtcaca tcgtctactg cccagatggt gaatttgcct
    15481 tcttctaccg ctttcaccag ttcactgtga agacttaaat ggcgaacgtt agctgtgggg
    15541 ataatgacac cttgtttccc ggttaactca cgttgctggc aaatagcaaa gaagccttcg
    15601 attttctcat ttaaaccacc gaccggctgg gcgcgaccga actgatcgac tgaacctgtg
    15661 atagcgatac tctgattcac cggcacatcg gcgagggcgc ttatcagggc gcagagttca
    15721 gccatcgagg cactatctcc atcaacttca ctgtatgact gctcaaatgt cagcgatgct
    15781 gagaagggga tctgttgctc aagctgtagt tccgacatca ggaacgcttg catgatcatc
    15841 atccctttcg catggatatt gccgccaagt tccgctttgc gttcgatgtc ggtgaattca
    15901 ccatcgccaa tatgcacaac gctgctaatg cgagaaggtt cgccaaaagc gcgtggatga
    15961 cccggaaatt caatgaccga aagggcgtta atttgcccga tgcgttcgcc ttcggtttca
    16021 atcaggattt gctcctgaag gatctcatcc tgcatacgtt cagcgaggaa accttcgcgc
    16081 cattcacgct gctgcagcat taagtttagc tgctcgccgg agaaggtgtc gccatcacac
    16141 agggaggcga cctctttaca ctggcggagg atccactgcg ggctaagcgg aagtgtttct
    16201 tgttcaccgg tgtagcgtgc tgcttcgcgg ataagtaccg gccaggcatc cgctcccggg
    16261 gcaggtaagt gattatgtct ggcggtaaat gtcacccagc gacaccactg gcttactgac
    16321 tccgcatcga caatctgcag agtatcttca aattcgctat aaatagcctg ctctgaaagc
    16381 tctggctcca tctcctggaa atcagccaat gattcgcgtt cgcctaccag aatgactttc
    16441 agcttcaatg gcatcgaagg cacagagacg gggagagggc gcgactcatc aaacgcaacc
    16501 cagtcaaaac gctcgcggtt aacgatattt ttcagccgca tccacagcag aggttgcgcc
    16561 agcagtgtac gcaaagagat gatgagaata ccgccatttg cctgatgcac cagaccaggc
    16621 tgcagggtaa tgtcgccatt aaactggcgc aggcagccaa agagttgctc cgcttctacc
    16681 cagtcggcag cgacaacttg cgttaaagtc gcaaaattat catctgcact cactgcgtga
    16741 cgtaagcgga tggagtggcc ggaaacctca tagtgatcgc ccaccagttg gcctgcatcg
    16801 ctttgtagcg tacgcgcggc atcggcaata agattcagat actcagactc ttccggggcc
    16861 ttagccagca taaaagagga ggatgctcgc gtatgcagca actgctccag cgcaaattgc
    16921 agccgcggtt gagtatcact gaataaagga tcgttttcgt caatcaaatg tggctgagca
    16981 aatatttcct gatagctatc ggtatcagga accaggtcac gccatgcaag tttcgtaatg
    17041 gtcaaagttg atgtttttta gtctgttgtc aaagccgcga ttataccgta accggcacta
    17101 cagcacacgt agaaagcacc gacaatactt ctggcatggg cgttaaagct cacaggatgg
    17161 agattctttt cttcactggc ctaaaaagct gatattctgt aaagagttac acggtaacat
    17221 tgagatcgct atgaaatatc aacaacttga aaatcttgaa agcggttgga aatggaagta
    17281 tctggtgaag aagcatcgcg aaggggagtt aattacccgt tacatagaag ccagtgccgc
    17341 ccaggaagcc gtcgatgtgt tgctctcgct ggaaaatgaa cccgtgctgg taaatggctg
    17401 gattgacaag catatgaatc cggaactggt caatcgcatg aagcaaacca ttcgggccag
    17461 acggaagcgc cattttaatg cggaacacca gcatacgcgc aaaaagtcga tcgatctgga
    17521 atttattgtc tggcaacgtc tggctggtct tgcgcagcgt cgcggtaaaa cgctttctga
    17581 aacgattgtt cagctgattg aagatgcgga aaacaaagag aaatacgcga ataaaatgtc
    17641 ttctctgaag ctggatctgc aggcattgct gggtaaggaa taactgacga aagtcagttc
    17701 aatttactaa aggcaaaaaa aaccccgcag cagcggggtt tttctaccag acgataactt
    17761 aagcctgcgg ctgagttaca acgtctttga tacctttaac ttcgatctct acgcgacgat
    17821 ccggagccag gcagtcgatc agtgcagcac gctgtttcac gttgtcacag gtgttgccag
    17881 taaccgggtt ggattcgccc ataccacgtg cggagatctt gtctgccggg atacctttgg
    17941 agatcaggta atcaacaaca gactgagcac ggcgctcgga cagaccctgg ttgtaagcgt
    18001 cagaaccgat gcggtcggtg taacccagaa caactacgga accgtctttc ggatccaggt
    18061 tgctcagctg gctgtacagc tgatccagag cagcctgacc ttccggtttc agggttgctt
    18121 tgttgaagtt gaacagaacg tcagacttca gagtgaagtg cttggtctgt acttccggtg
    18181 ccggagctgg agccggagca actactggag ctgcttcgcc ctgaccgaaa cggtaggaaa
    18241 cacccaggct cagcatgccg ttgtccggac gagtgccgat ggtgtgtgcg tcaccgatgt
    18301 tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg tactcaacac
    18361 cgccagcgaa gaccggagaa acgccggtgt cgtggttttt accataaacg ttggatttag
    18421 tgtctgcacg ccataccata ccacccagac gagtgtagat gtccaggccg tcagtgattg
    18481 ggtaacccag tttagcggtc agttgaacgc cctgagcttt gtatgcaccg ttttcaacgc
    18541 tgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaagcca acatacgggt
    18601 taacctggta accaccaaaa gcaccagcgc ccagttggtt ttcatgggtc gggccattgt
    18661 tgttgatgaa accagtgtca tggtactggg accagcccag tttagcacca gtgtaccagg
    18721 tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg
    18781 cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc catgaatatc
    18841 tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat taactctacc
    18901 gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa
    18961 cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag gtatgtttaa
    19021 tcttttttgt cagcgacaat ttacagaaga aaatcgcgga aaccgcttca gacaagcctc
    19081 cgcaaggaaa attagtcacg actgaaagca ttggctgggc gacaaaaaaa gttccaggat
    19141 taatcctaaa tttacttaat gatacaaatt agagtgaatt tttagcccgg aaagttgtct
    19201 cgtggcgtga gaggatgcgc ttaccggacg cataataaac cccatagcgt taccttcatt
    19261 tgccgcatca acaagttcag catgctcttc ttcagtcaaa tcatctgcca accaaccgat
    19321 caccacactg taattgcccg tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg
    19381 ggagagctgg ctaatctgca ttactttcgt taagggtagc ccagatgcct gaacccattc
    19441 ccgactcagt ttttgttgcg gtgttaacca gagttgccag cgcgattgct gaccgagttg
    19501 ctgtaacaat ggcaacagta gaagttgcgt catcatgggc tgatcttcgc gatagacaac
    19561 ttcactgata agcccggctg tagtgttttc cgtagagaca cgcgcaattt tacttgctgc
    19621 ggatgagaac gacgaagaac gatgtgcata gcctgaagtg tacataatca atccagcccc
    19681 tgtgagttac tgtatggatg tacagtacat ccagtgacaa caaagatcaa ccctattttc
    19741 ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa cataaattaa tcttgcccct
    19801 taagaataag ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc attcttttat
    19861 gttatgattt taaaaggaat tttatgaaaa gcctctccta taagcggatc tataaatcac
    19921 aagaatacct ggcaacgttg ggcacaattg aataccgatc attgtttggc agttacagcc
    19981 tgaccgttga cgacacggtg tttgcgatgg tttctgatgg tgagttgtat cttcgggctt
    20041 gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt
    20101 gtggccgatc cgttaccctc aattactatc gggttgatga aagtctatgg cgaaatcaac
    20161 tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag aaaagcacgc
    20221 gcaatacccg ggaaagactg aaagatttgc ccaatatgtc ttttcatctg gaagcgattc
    20281 tcggggaggt ggggattaag gatgtacggg cgttacgtat acttggggca aaaatgtgtt
    20341 ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa gattctgttt atgcttgaag
    20401 gtgccattat cggcattcat gaagctgcgc tcccggtggc acgccgccag gagcttgcag
    20461 aatgggctga ctcacttacg ccgaaacagg agtttcctgc ggaacttgag taatctggcg
    20521 ttgcagacga ccaatctcag gcagtaatgc aatcaataat ccaacttgtt gtacgaccag
    20581 aggttctttg ctgtctgccc gtggttcaag ttgctgcatc cgctgtttca ggctagctaa
    20641 tgcctcattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat caacatagca
    20701 aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc
    20761 gagggctgag atatagctgg taaacgtatg gttaaggcac agcaaccgaa acgcggcttc
    20821 gcgaatttgc ggggtaacgt tcggctcgct ggacatattt gataccaccg acgccagctc
    20881 agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc
    20941 ctgatggtat tgctccagta tggcatcgag ataccgacag ttggcctctg tggcgcgttc
    21001 gagcatgcgc ggcagattgc gaaactgcca gtcaggccag atgtagctca ctgccgccca
    21061 cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc
    21121 acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgagcgta
    21181 ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc
    21241 ttccagtgat ggcacaaacc acagcacagg aatgccaatg gcgataccta ccagcgtacc
    21301 aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa
    21361 acttgtcagc aagatccaaa acccgtgatg cattccggtt atttgaatga tggcgtagcc
    21421 gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa
    21481 gtgacggcta agacgcagcc agatatcact caacccgtgc gggctgtcat cagcgagctc
    21541 attttcgtca ttattatggg gtagtgcctg ggcctgttct gattcaattg ttgccagttg
    21601 ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg
    21661 tgcgccgtta tcgcgcatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa
    21721 atgcggatca tgttgataag gctgacgcaa caaaatacag cgtgacagtt gctggcacgc
    21781 ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg
    21841 ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc
    21901 gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg
    21961 ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agcagaccgt tggcgagagc
    22021 caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt
    22081 gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg
    22141 gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg caccggccag
    22201 cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat
    22261 ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa
    22321 aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag
    22381 caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa
    22441 acgccccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag
    22501 cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc
    22561 aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg
    22621 acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg
    22681 cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc
    22741 ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat
    22801 gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt
    22861 caataccgta ccgccagtat tcagcagcac atttttgcca gccgggttca gttcatcgac
    22921 atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg
    22981 tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag
    23041 agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc
    23101 tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca
    23161 cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg
    23221 aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga
    23281 tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaata
    23341 aattactggc gattcactgt aagcgtggtc tggtatgggg cgagctggaa tttgtactgc
    23401 cggacgaaaa agtggtgcgt ctgcacggca ccgaatgggg cgagacgcag cgtttttacc
    23461 atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg
    23521 ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc
    23581 gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc
    23641 cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct
    23701 ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc gaagccatgc
    23761 ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat ccggcgcagg
    23821 cccgggcagt cgttaatggc gagcattctc tgttagtgct ggcaggtgca ggaagcggaa
    23881 aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc acgtggtgaa gcgtcccctg
    23941 agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac gagcggattc
    24001 gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgctg gcgctgcata
    24061 ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg
    24121 cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg
    24181 cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact
    24241 tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc tgggtaagtc
    24301 tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc
    24361 gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg
    24421 cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg attgtgattc
    24481 tggagaaagg tcgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata
    24541 tctcgccgca gcgggcagcg ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt
    24601 tgttcgctgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc
    24661 tcaccaccgc tttccatgaa aactttggtg aaggcgaacg ttgtgattta gacacgactt
    24721 accgttttaa cagtcgtatc ggtaaggtgg caaaccgttt tattcagcag aacccaggcc
    24781 agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc acgttattgg
    24841 atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa ccggaagagc
    24901 gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa
    24961 cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg
    25021 cggattacgt catcatcgtt ggcttgcagg agggaagtga tggttttccg gctgcggcgc
    25081 gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag
    25141 aacggcggtt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta
    25201 acaaagagaa tccctctccc tttgttgaaa tactgaaaaa tctggatgtg ccggtggcga
    25261 gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtccgc
    25321 gagataacgc tgataatcgg ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg
    25381 gattatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgtcgccag
    25441 acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat cccagaagaa
    25501 aatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat
    25561 tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatta agttaccggt
    25621 agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct
    25681 catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa
    25741 agtgcgagtc gtcagttcca taatgtacat ccgtagtcaa ctttcctaca gattactgta
    25801 agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggtaca aaaatctcag
    25861 gaagatggtt gtttttccgc ccactgcagg aaagtatttc gcgtttgtgg gtcagccagt
    25921 ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc
    25981 agcttggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt
    26041 agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt
    26101 tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca
    26161 tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg
    26221 cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac caccagcggt
    26281 ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac
    26341 actaactgat gcggtccatt atccagttca atgctgtccg caccgcgcag cagagagctg
    26401 gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg
    26461 gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc ggttttcata
    26521 ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc tttaatcata
    26581 cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat tagctgcatg
    26641 gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat aatgaaagaa
    26701 accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg ggcaagcgac
    26761 aaacccgatc gtcccagcta tcgcgtgatg aaatatctac tcgaccaggg gtatcatgtc
    26821 attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg gtatggcacg
    26881 ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc agaatcggcg
    26941 tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat gcaactgggg
    27001 gtgattaatg agcaagcggc agtactggca cgggatgccg ggttgaatgt ggtgatggat
    27061 cgctgcccgg ctattgagat ccctcgcctg ggcctggcca aataaaaaat ccccggaagg
    27121 caaaaacctt ccggggattc gttcagggat cagttacgca gacgcggggc ctggagttgt
    27181 ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat
    27241 tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc
    27301 atcactacgt gataccacgg agcggcgcga agctcgtcat ttaccgccag ctcatcaggc
    27361 gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag ataacctaac
    27421 agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg
    27481 gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag
    27541 gcaaaccctt tcagatatag cccttccgga taggtagcga tcaccggatg atcggctgcc
    27601 tgacggaact gctctataaa ttgtacatca cggccggcat caattgcggc atccgcgatg
    27661 attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaataccg
    27721 ccttcattca gcaactgaat cgccagcatg ttgatatctt tatagccacg acacgcgccc
    27781 atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac gtcaaatttt
    27841 tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac aaactcagcc
    27901 ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctgg
    27961 gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca
    28021 ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg ggtagccagg
    28081 cggctgtctc gctggtccag gtagtagccc gttttgtgtc cgtgctgaat atccaccagc
    28141 agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg
    28201 cccagggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc
    28261 gcacattccg ggtacagcgt ttgcagggca ctaactaatg ccgcgcgctg atattctgcc
    28321 ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc
    28381 aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagc
    28441 cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac
    28501 tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg
    28561 cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta
    28621 ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt
    28681 tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag
    28741 aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg
    28801 agagcagcaa tgtcgaaagt ctgcataatt gcctgggttt acgggcgagt tcagggcgta
    28861 ggatttcgct acaccacaca gtacgaagcg aaaagactgg gtttaaccgg gtacgccaaa
    28921 aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca ggtggaaaaa
    28981 ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc
    29041 gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat acattttacc
    29101 ggttttggca ggccagcaat tttggtggct tgcttcgccg gacctttcgg aaacagtcgg
    29161 tataaatagc ggctgttacc tttttcttcg ccaaatttat tcgccatcgc ttttaccagc
    29221 atacgaatcg ccggagaagt attgaattcc agatagaaat cacgcacaaa acgcaccact
    29281 tcccagtgtt ccggtgacag cgaaatccct tcgttctctg caatcaccac tgccagcggc
    29341 tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct
    29401 tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta acaaaaaaca
    29461 aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc gcggctggcg
    29521 aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata caggctaacc
    29581 gtggcacgaa tatagttcgt ttcaccgcca tgaacgatgt tgctggtttc aaacaaaata
    29641 gcgccagagg agatcaaaat gaagaccgcg ctgatcgcca gatgcagagc aggctgctgc
    29701 aggaagatat tagcaaccat accaatcagc accaccacaa tacccgccat cagcataccg
    29761 ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca gaagaacact
    29821 aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga cagataggtg
    29881 ttcagaatag gtccgaggat ataacccaga aaaccggtaa aggcgaatgc ggagataatc
    29941 ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat acccaccagc
    30001 gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc
    30061 gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt atgggtgcta
    30121 agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc actctctatg
    30181 acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag aagttttacc
    30241 catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa
    30301 tataacgccc tgagaatttc gacaggcaaa agaaaaaggg gttagcattt agctaacccc
    30361 ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt
    30421 ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca
    30481 tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc
    30541 attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc cttctctttt
    30601 actcgtttag caaccggcta aacatcccca ccgcccggca aaaagaaaaa taggtccatt
    30661 tttatcgcta aaagataaat ccacacagtt tgtattgttt tgtgcaaaag tttcactacg
    30721 ctttattaac aatactttct ggcgacgtgc gccagtgcag aaggatgagc tttcgttttc
    30781 agcatctcac gtgaagcgat ggtttgcctt gctacaggga cgtcgcttgc cgaccataag
    30841 cgcccggtgt cctgccggtg tcgcaaggag aagagacgtg cgatatgaat aacgaggaaa
    30901 cattttacca ggccatgcgg cgtcagggcg ttacccggcg cagctttctc aaatattgta
    30961 gtctggctgc cacgtcgctg ggattaggcg cgggaatggc accaaagatt gcctgggcgc
    31021 tggagaacaa accccgcatt ccggtggtat ggatccacgg tctggaatgc acctgctgta
    31081 ccgaatcttt tatccgctcc gctcacccac tggcgaagga cgtcatcctt tccctgattt
    31141 ccctcgatta tgacgatact ttgatggctg ccgccggaac ccaggcggaa gaagtcttcg
    31201 aagacatcat cacgcaatac aatggcaaat atatcctcgc agtagaaggt aatccgccgc
    31261 tgggcgagca ggggatgttc tgtatcagca gcggtcgacc gtttattgag aaactcaaac
    31321 gtgccgctgc cggagccagc gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg
    31381 tgcaggccgc gcggcccaat ccgacgcagg caacgcctat cgacaaagtc atcaccgaca
    31441 aacccattat caaagtacct ggctgcccgc cgatcccgga tgtgatgagc gccatcatta
    31501 cttacatggt gacctttgat cgcttgccag atgtcgacag aatgggccgt ccgctgatgt
    31561 tctatggtca gcgaatccac gataaatgct atcgccgcgc ccacttcgac gccggagagt
    31621 tcgtccagag ttgggatgat gacgctgccc gcaaaggtta ctgcctgtac aaaatgggct
    31681 gtaaagggcc taccacctat aacgcctgtt cctccacacg ctggaatgat ggcgtttctt
    31741 tcccaatcca gtctggtcac ggctgcctag gctgtgcgga aaatggtttc tgggatcgcg
    31801 gttcgttcta cagccgcgtg gtcgatattc cgcaaatggg tactcattcc accgccgata
    31861 ccgtcggttt aaccgcgctt ggcgtggtgg cagcggctgt tggtgtgcac gcagtcgcca
    31921 gcgccgttga ccagcgcaga cgtcataacc agcaacctac agaaaccgaa catcagccag
    31981 gcaatgagga taaacaggca tgagcactca gtacgaaact cagggataca ccatcaataa
    32041 tgccggacgc cgcctggtgg tcgacccgat tacgcgcatc gaaggccaca tgcgctgcga
    32101 agtgaatatt aacgatcaga atgtgatcac caatgccgtc tcctgcggca ccatgtttcg
    32161 cgggctggag atcatcctac aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg
    32221 tatctgcggc gtctgtactg gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc
    32281 tatcggtatt aaagtgccgg acaacgccaa tatcatccgc aacattatgc tggcaacgct
    32341 ctggtgccac gatcatctgg tgcacttcta tcagcttgcc gggatggact ggatcgatgt
    32401 gttagatgcg ctgaaagccg acccgcggaa aacctccgaa ctggcgcaaa gtctctcctc
    32461 ttggccgaaa tcatcccctg gctatttctt cgacgtacaa aaccgcctga aaaaatttgt
    32521 tgaaggcggg cagttgggga tcttccgcaa tggctactgg gggcacccgc agtacaaact
    32581 gccgccagaa gctaacctga tgggctttgc ccactatctc gaagctctcg atttccagcg
    32641 tgaaattgtc aaaatccacg cggtctttgg cggtaaaaac ccgcatccaa actggattgt
    32701 cggcgggatg ccttgcgcca tcaacattga cgaaagcggc gcggtcgggg cagtcaatat
    32761 ggaacgcctg aacctggtgc agtcaattat cacccgcacg gcggacttca ttaacaacgt
    32821 gatgatcccc gacgccttag ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac
    32881 tggtctttct gataaatgcg ttctcagcta cggcgcattc ccggatattg ccaacgactt
    32941 tggcgagaaa agtctgctga tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt
    33001 gctgccagtg gatttggttg atccgcagca ggtgcaggag tttgtcgacc acgcctggta
    33061 tcgatatccc aacgatcagg tcgggcgtca tccgttcgat ggcatcaccg acccgtggta
    33121 caaccccggc gatgtcaaag gcagcgatac caacattcag cagctgaatg aacaggaacg
    33181 ctactcgtgg atcaaagcgc cacgctggcg cggtaacgcg atggaagtgg ggccgctggc
    33241 gcgcacgtta atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat
    33301 gatgtcggcg ttgaacctgc cgctttccgg tatccagtca acgttaggcc gcattttgtg
    33361 ccgcgcgcac gaagcgcagt gggccgcagg taagttgcag tatttcttcg acaagctgat
    33421 gaccaacctg aaaaacggca atctcgccac tgcttccacg gaaaaatggg aacctgcaac
    33481 ctggccgaca gagtgccgtg gtgtcggttt taccgaagcg ccgcgcgggg cgttaggcca
    33541 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
    33601 gaacgccagc ccacgcgatc ccaaagggca gattggagct tatgaagcgg cgctgatgaa
    33661 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
    33721 cccgtgcctc gcctgttcaa cacacgtgct gggcgacgac ggtagcgagc tgatctccgt
    33781 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
    33841 ccactatgtc tttgaagcgc cagtccgcat ctggcactgg ttgacggtgt tatgcatggc
    33901 ggtgttgatg gtcaccggat actttatcgg caagccgcta ccttccgtca gcggcgaggc
    33961 gacgtatctg ttctatatgg gctacatcag gttaattcac ttcagcgccg ggatggtttt
    34021 taccgtggtt ttgctgatgc ggatctactg ggcttttgtt ggcaatcgat actcccgcga
    34081 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
    34141 ctggtatctg tttctggcaa aacgtccgag tgccgatata ggccataatc ccatcgccca
    34201 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcactg gttttgcgct
    34261 gtacagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
    34321 ctactggacg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
    34381 gattggcgcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
    34441 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
    34501 taacaaggag tgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
    34561 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
    34621 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
    34681 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaacg
    34741 ctgcgaacct atgccggaga acgcattccg gctgatatca gcgcgaagaa aatgagcctg
    34801 catcagaaca gtttctccga agtgctggcg ctggcagata tccgcggaca tctgccagca
    34861 catattgccc tcgtcgggct gcaacccgcg atgctcgacg actacggcgg cagcctgagc
    34921 gaactggcac gggagcaact gctcgctgcg gaacaatcgg cgctggcgca gttggctgcg
    34981 tggggaagtg tgccgcaacc ggctaatgaa tcgcgttgtc tcaattatga ctgtctgtcg
    35041 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
    35101 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
    35161 cggtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtggtgttat
    35221 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
    35281 tactgcacga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
    35341 aagccatcgg cgatcgcttt ggcgtctttc gctttcctac cactttagtg tttaccggcg
    35401 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
    35461 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacttttt tccatctgct
    35521 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
    35581 tcaggtgaat gatgaaccga gtatggcggc cctggagcaa tgtgctcaca gcccgcaggt
    35641 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccagccgc cgttgggcga
    35701 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
    35761 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggat gacgacagta aaagtgaaat
    35821 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
    35881 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
    35941 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
    36001 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
    36061 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
    36121 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaacg ccacggggtt
    36181 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
    36241 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
    36301 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
    36361 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
    36421 tcaggataaa gagggagatc taacattatc gggttatttt tctctcttcg cctacaggag
    36481 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
    36541 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc atggaaacca
    36601 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
    36661 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
    36721 ctaactgggc attctattcc aactatgtgg gcgatatttt tggcgcaccg ctggcgatgg
    36781 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gcttttcttc ttcggctggc
    36841 agcggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
    36901 tctctgcatt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
    36961 ttgatatcga caccctacgt atggagatga ccagcttcag cgagctggtc tttaacccgg
    37021 tcagccaggt gaaatttgtg cacaccgtaa tggcgggcta cgtgaccggg gccatgttta
    37081 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtt gcattacgct
    37141 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggcacgcta caactcggag
    37201 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
    37261 agtggcaaac ggaacctgct cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
    37321 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgtt agggatcctc gccactcact
    37381 tattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
    37441 aacgcggacg tatggcttgg ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc
    37501 atgtgttgca ggcattccgg gaactggaag gtgacctggg ctacggcatg ttgctctccc
    37561 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
    37621 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
    37681 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
    37741 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
    37801 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
    37861 catactccgc gcattccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
    37921 tagggcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
    37981 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
    38041 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
    38101 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtctgctgc cgctggtggc
    38161 gcgtaatgat gatgaacgcc ggatagtgat aaacagcgtt ggtgcacact gggaaggcaa
    38221 ccaggtctgg ttgatcctcg ctggtggggc attatttgcc gcctggccca gagtgtatgc
    38281 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcac tgttcttccg
    38341 cccgctggcc tttgattatc gcggaaaaat cgccgatgca cgctggcgta aaatgtggga
    38401 cgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    38461 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atctcggcag
    38521 cttctggcaa ctgctgacgc cattcccttt attgtgcgga ttgctcagcc ttgggatggt
    38581 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    38641 acagctggcc accaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta
    38701 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    38761 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga
    38821 atcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    38881 ggcgatttat cgtggtcgcc caggttgggg atttttaatg gcgtcattga tgcaatttgg
    38941 cgtgattttc acggcaggca tcacgctgtt ccccttcgtc atgccgtcaa gcgtgagtcc
    39001 gatctccagc ctgacgttgt gggacagcac ttccagccag ctgacgctga gcattatgtt
    39061 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    39121 aatgtggggg cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
    39181 agcaggaaca atgtggtatt tactttggtt cgtcggcatt ttgttgatgt gttcgctctc
    39241 cacccttgtg ttggtatggc tggacccgcg tctgaaaagt taacgaacgt aggcctgatg
    39301 cggcgcatta gcatcgcatc aggcaatcaa taatgtcaga tatgaaaagc ggaaacatat
    39361 cgatgaaagc gatcttaatc ccatttttat ctcttctgat tccgttaacc tcgcaatctg
    39421 cattcgctca gagtgagccg gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg
    39481 gtgtgcgtgc tccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc
    39541 caacctggcc ggtaaaactg ggttggctga caccgcgcgg tggtgagcta atcgcctatt
    39601 tcggacatta ccaacgccag cgtctggtgg ccgacggatt gctggcaaaa aagggctgtc
    39661 cgcagcctgg tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg
    39721 aagccttcgc cgctgggctg gcacctgact gtgcaataac cgtacatacc caggcagata
    39781 cgtccagtcc cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataatg
    39841 cgaacgtgac tgacgcgatc ctcagcaggg caggagggtc aattgctgac tttaccgggc
    39901 atcggcaaac ggcgtttcgc gaactggaac gggtgcttaa ttttccgcaa tcaaacttgt
    39961 gccttaaacg tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac
    40021 tcaaggtgag cgccgacaat gtatcattaa ccggtgcggt aagcctcgca tcaatgctga
    40081 cggagatatt tctcctgcaa caagcacagg gaatgccaga gccggggtgg ggaaggatca
    40141 ccgattcaca ccagtggaac accttgctaa gtttgcataa cgcgcaattt tatttgctac
    40201 aacgcacgcc agaggttgcc cgcagccgcg ccaccccgtt attggatttg atcatggcag
    40261 cgttgacgcc ccatccaccg caaaaacagg cgtatggtgt gacattaccc acttcagtac
    40321 tgtttattgc cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact
    40381 ggacgcttcc cggtcagccg gataacacgc cgccaagtgg tgaactggtg tttgaacgct
    40441 ggcgtcggct aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac
    40501 agcagatgcg tgataaaaca ccgctgtcat taaatacgcc gcccggagag gtgaaactga
    40561 ccctggcagg atgtgaagag cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc
    40621 aaatcgtgaa tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc
    40681 agttacctga atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga
    40741 aaaggcgttc acgccgcatc cggccatttt cagttttact ctttctcgga gtaactataa
    40801 ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg
    40861 atagcgcctt taatattgac gcctgcctgt tccagacgct gcattgacaa actcacctct
    40921 ttggcggtgt tcaagccaaa acgcgcaacc agcaggctgg tgccaacaga acgccccacg
    40981 accgcggcat cactcaccgc cagcatcggc ggcgtatcga caatcaccag atcgtaatgg
    41041 tcgttcgccc attccagtaa ttgacgcatc cgatcgcgca tcagcagttc agacgggtta
    41101 ggtggcacct gaccgcgagt aatcacatca aagcctcctt tgccaaaatg ctggatcact
    41161 ttgttgagct catctttacc tgccagatat tccgacaagc catgttcatt actcacggta
    41221 aacaggttat gcgaataacc acggcgtaag tcggcatcaa taaataacac tttttgatcg
    41281 gactgggcga tcaccgctgc cagagttgaa ctgacaaacg ttttaccact gtctggcgtc
    41341 gcaccggtga tcatcagaat gttattctcc gtctccatca tagcgaagtg cagactggtt
    41401 cgtagcgcac gtacggcttc cacagcagaa tccgccgggt tatccaccgc caggaagggg
    41461 atatttttag tacgatggcg ctgctgatta gaaaataaat ttttcttacg cagacgggta
    41521 cgtttatcca gccactcgga cattgggata gtggcataaa cgctgatgcc gtgctcttcc
    41581 agttgttccg gggcttctac accacgacgc aacatcgcac gcgccagcac ggcacccacc
    41641 gaaataaaca ggccaagaat aaaaccaagc accacgttca acgctttttt cggtttcact
    41701 ggctgcggct gagtgactgc cgggtcgata atccgcacgt taccaatggc actggatttc
    41761 gaaatactca actcctgctg gcggttaagt aattgcagat ataccgcacg gcccgcttct
    41821 acgtcacggc ttaaacgtaa cacttcctgt tgagtggaag gcattgccga tacccgctta
    41881 ttcaggcgtt tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc acgataagtt
    41941 gggtgatctt tcttatacag ctgggagatc tctgcctcgc ggaaagtcag ctcattgagt
    42001 tgattatcaa cgttcacaat ctgctcaaga acggctttgg cttctaggtt aaggtcaacc
    42061 gaatcgcgct gctggcgata aacgttgagt ttttcttccg cctggtccag ctcgctgcgc
    42121 acttcaggca actggcgctg taagaattca aggctttgtg aatcctgcgc cgcctggcga
    42181 gcgatattct gttgcaaata gttgttagcg atgctgttca gaatacgagt aatcaactgg
    42241 ggatcatcac cagtcatggt aagttccagc atcccgcttt ctttactgcg ttcgctaacg
    42301 gtaaaggttt cctgcaatgc gttaatcgct tccagttcgg tacgctggct caggacaaac
    42361 tgtgttcctg gtttggcctt aatgtccgcg atagtcagcg caacgccatc tttttccaga
    42421 cgctggccga ccataccatt gacggtgaac tcttcacctt ccagtgtata gtggccgttt
    42481 tccccaaccg tgagtgtcag ttgctgatcc tgaccattca gttgtggaat atgcatccag
    42541 ctgatcgcca gctcacctgg tttttctttg gttaatctcg cccagccgcg acccacaatc
    42601 ggaaaatact tctgctcaac tatgtcgcgc agattcagtt cagcaatggt tttaccgaga
    42661 atcatgcgcg attgcagcag ttggatctcc ggtgcagact cgggcgatga gttagggatc
    42721 atatcactca ggccgctgag aatggcgttg ccctgttttt gctcaacctg gaccagagta
    42781 tctgcctgat aaattggtgt gcttaacagc gagtaagcga cagcgatcag cgtgaataac
    42841 gcggtcacgc tgataataaa cttacggtga tcccataact cgccgaccag acgaagcaga
    42901 tcgatctcat tttcctgagt gctgcttggt ggcgtattca tatttttagt tgtcatcgta
    42961 ttttataaac tcaaattacc ggctgaggcg cttcgcccat tcctgactgg cgcgctccaa
    43021 cataccgtag acatgttcaa atgcgtcctg acttttacga tagggatccg ggatctcttt
    43081 ctgttccagc cattgcccaa acagcattgt cttcccgcgg acctcgggag cgattgcggt
    43141 aacctgagca atatgctctg actccattgc cagaatcaga tcgtaatttc gtgccatctc
    43201 agcagtgagc ttacgtccgg catgtccttc aagagatacg ccatgattag cggcaacatc
    43261 tgctgctgtc gcatctgcag ggtgttttac cagaccatga acacctgccg atttgacttt
    43321 tacgcctggc agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc aaatgttgcc
    43381 ggtacaaacc accaggattg agttaaattt tagttgggcc atgtttgaat ccaccgtgaa
    43441 gtctctgtca ggtcatgcac cccggaaatc gtcggtacca gcagagaaat cacacggttc
    43501 caacgagcca gcggcgcggt agtgacataa acaatgtcgt aaggttcaag ctggaattcc
    43561 gttccgagta tcattgccga tgcgtccttc gcattcagct gataaatatt ggcgattttg
    43621 ccattctgct tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc agcgacatcc
    43681 tgattcatac cctcagcgtt acccagcgct tccgccaggg tcataccgct gcggtccatt
    43741 ttcagcgtgc tctgtttacc cacttcaccc atcacgaaga ctttcagatc atcgttgcgc
    43801 ggaataaata aaatatcgcc cgggtgcagc agtttgttct ggcacaagtc gccacgctgc
    43861 atcagcgcat agaggttcac tttggtcttt acgccattct gggtgagcac cacgttgcgc
    43921 cagtctgcat ctgcggtcag accgcccgcg gcgttaatcg catccatgat ggtcagagga
    43981 atattggtaa tcggctgctg acccgatttg ctgacttcgc cggtgacgta cgctttttgc
    44041 gatcggaacg ccgcgacgct gacatcgact tgcgggcttt caatgactga atcgagacgc
    44101 gctgtaatct cgttgcgtac ctgggtaagt gttttcccgg ccactttcag gcgaccaata
    44161 taggggtaga aaatcgcacc atcggcgtta acccagttgc cggtatcact ggcgctacgg
    44221 tattgccctg cgggggtggt tagttccggg tgatcccaga ccgtaaccat caacacatca
    44281 ccaatcccga tacgatattc atagtttgcg atttgctgct ccagctccgg gttagcctgc
    44341 gacataatcg gtttggcgcg caactgatca atcaatcctg gcgtgacggg atagatattg
    44401 accattttgt ccaggtcata ctgattgtca ggtagttcaa tgacatcctt gttactggtc
    44461 gataaattct gaccaggaac aagggtacat gctgtcaaag acagaacagc caatgtcagt
    44521 accgaaaact taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa tgtaatgtta
    44581 tgaattacgt caggttattg tccaaccggt acttctcgct ctgccgtcat tggatagagc
    44641 tggtatttgc gtgacagcat ttgtccacca tcacgcgtca atggtgtcca cgaaaccacc
    44701 gtgcggttgc ggttaggtcc aatggtcatc aagtcgagtg gaatcgagat ataaaaacct
    44761 ttactaaagc cgccttcgcc gtagtcatct ttagatacgt tactgattgc cgcccatacc
    44821 cctaccgcca cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcgcc tttatctttt
    44881 gccagatatt gcccaacgct aagtttcatc agtacgccat tgagcgtcgg cgggttccag
    44941 taagccgtca cgaaaccagt tggcgtggaa taatcggtga agcgcatcat gttgtcccag
    45001 tcacgttgct tcacgtagtt aacgtccaca cccagcgccc agctggcatc tagcgggcga
    45061 taaagcagct cggaaccgac accggcgtac atcgtttcca gataaccgcc atacacctgg
    45121 ccatagaaac cattgcctaa gtcggcaaag tagttcgcct gcaagttgtt gagataaacg
    45181 tcattgcgaa cgtaatcacg gatatgcgtg cgcacgcggg gcagggtaga gtccgcgggc
    45241 aacagcgaag acttaaactt gtcgtagttg ttgtaaatat tggtgaaaat accgccatca
    45301 agcagcaggt ggtcggtaaa ccagtagcgg gcactggaca tcagccccag ctggaacata
    45361 tagaaatctt ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga gtagctaaag
    45421 cgatcttcac gaatacggta gccccgacca aaggcagaaa gatcttctgc ttcaacacgt
    45481 tgttgttgaa gttgctctga ttgaccaggc gctgtaccag ccagctgttt gcgcaggctg
    45541 gctacgtcgg tttcggtagt caccatcgcc atatgctcgc gcttttgcgt cacgctaatc
    45601 ttctcaacgc cttgcggcag gttattcacc agaatccggt tggcacgatc gaccgcttcg
    45661 cgagaatcac ggtatttgta ttgttgacca gacatataca gcgtcttatc gcgcagctga
    45721 atttccggtg cttcaaaacc tgcgttgtac ttcagcgcgg taagttggtt cgctactgtg
    45781 gtgtactgca atccttcaga ttcaggcgca ggttgatatg ccggttttgg cgtatcgcgc
    45841 agggcagggc gcagatcgtt gaaattggtc cgtaacgtga agccaaacat caacgtgtta
    45901 ccgcgttcat aactcaggtt gagatctgcc cagctggcag cgcgataaac tgcgccgacg
    45961 ttgaaatggc ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt gttgccatcg
    46021 tattcgagtt tcagacgcag gggattccac ggcgtttgat actcaatgcc gccaaagatg
    46081 gaagccgggc cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg agactctgcg
    46141 cgatgacaat atttatcgct cacccggcaa aacgggttgg taatattgcc cgcattaccg
    46201 gcgtagcccc atgccatccc gagggtgaaa tcaaatggcc ccgcttgctt gctggccacc
    46261 agatactcac cgtcaaacag acccgtacca gcaatatcac gtttaccaaa cgccacttgc
    46321 ggtagccagt aaccttcttc ccacaggcga agtttaaaat cgaatgattt gtctttgtat
    46381 gactgatcgc cgctgaaatc ttcccactgg ctatatttgc gtgtgcgcac atccgtataa
    46441 cgaatggtgc cttccagcca tgggaacagc gccacggagg tggagtagaa ccggtattga
    46501 tcgttatccc ggtagttgac gctgaattca ccttccggtg cgatgcgcgc atttggcatc
    46561 tgcaacaatc ctgtgccgcc aaagtctgat tgcgacgaac ctagcggatc cgggtaggtt
    46621 aatacttcag catgacaggc actggagacg gcaatggcca ggcagcttaa aagataagaa
    46681 ttcttcttca tcagtcagga acacgttgcg ttaaaacaga cacaatctgg tcatttaaat
    46741 cagcatattt ttcgggcagt acatgcgcag aaaagcccaa ccaaagctgg ctgccaggcg
    46801 gtggttccac atgtctttta ttccacaggg caacaggggc gacgacagtc tcaccctcag
    46861 gcgtaatcac cataacgtta ttcttgtccg cgcctgcaag gcgggggtga tcttgcagat
    46921 aatcggtaac gctacggcct gcctgccagg gcaattgccc ggccccagaa accgcaccca
    46981 gcagagtaat agtcaccgga cgctgtacgg tatagagcgt atagtccccg accaacgggg
    47041 gattgctgtt ctcatccact cgaacaaaat cagggtccag tttgaccggt aagcgaccag
    47101 tgatattgag attgagtaac tgctggcgca cggatttaat cgttgccgcc acatcgtcgt
    47161 cagcttctgc ttcccaagaa gcaagctgtg ccatgacatg ttggtagtct ttaagcgctt
    47221 tagctttcgc cgcagagtcg gtcagcaagg cactaggcca ccagaggcga tcgcgcagtt
    47281 gcggttgtgt caccaactgg acaacatttt ccatggggcc aacggaaagt gtctgttgtt
    47341 cgccaggcag ataaatagtc accgttcctt gcgcaaaggc atggggtgtc attacgtaaa
    47401 gtacgctggc aatgaaatac gactgtaatt tattcatggt gcgggtttaa gaaacgtcat
    47461 ttctacggga ataacccctg cgcctaacat ttgccgactt tgacgaacct ggccagtagc
    47521 cgaatcgacc cagaatgtgt tttgccactg tttttctggc cgcgtggaag tcacctcttc
    47581 ctgccagaca ttacacagca cattgcgacc cgcaatattc agcgtctcta atccagcaaa
    47641 ggaaaaagta gaactcaggg tcgcagagcg aaaatggttg tcttcactcc agagaatatc
    47701 gcgcgtccag cggctgctat cttttattgc cagcgcgtta cgcagagggt cctgcccgga
    47761 atttgtcact tccagcagat tattattaag tttgacggtt tttaaaagac gtccattgtg
    47821 ggtaaccagc atggcgttat cctgggataa ccatttgctt tgttcttgtt ctatatatcc
    47881 cagtaccaca aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg aataaggcaa
    47941 cgcctggatc tgctgatccg ctacggtgat atcctgatta tcgaaaaggc tggcgcgaaa
    48001 tgtatcgacc atactttgct ggctgtgcgt acatcccgca agaaatagtg cgaaaatcga
    48061 taaaataaga gggcgcacct ttaatccttg aaaggttaat taaaaaaatg caaatcttta
    48121 aagcccggta cgaatatcgc aaccgggtat catttaactt taatttcgac gcgttaaagc
    48181 agtattagcg tgtagtggac gtggtggttg aggtgttatg agaaccgcca tcgccactgt
    48241 catttgctgc aacaactgcc aacgtcgcca caccaacagc cgttgccaca ccgacaactg
    48301 tcgccgtgcc gacagcgcca gcagccacgc caccagtggt agtagcagtc gtggttgcgc
    48361 cggtagtggt cgttgtggtg ccagtagtac ccgtcgttgc ctcggtcccg tttgttgctt
    48421 caggggcagc aaacgcagct ggcgtcgtca acatgaaggc cataaggatt gcagaaagct
    48481 tgtgtttcat aacttttcct ttattcatcg catggacaat acgtacgctt gagatcagga
    48541 ttgtctactt tgagtataac atccttgtga aatctccctt tcctcatacc gcttgcacta
    48601 tatactgcgg gtaggaaagg cgcgcagagg gaaatataag attgttaact atccccctct
    48661 ggaaatacga cttgtgagca taagataaat cttagcaaaa tacaaattaa agataaggaa
    48721 attgttacga aagctattaa ttattgctgg taatatagtt tcaagtggta ctataactgg
    48781 ctgttaaatg gcatttataa ctattaggtg cagagatatt cgcttaaatg gattagtttc
    48841 tttgtgaaaa agtaactgat gttataatat atggtgttga ctattgcact attccaataa
    48901 aaccagtcag ctttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg
    48961 gtcctcgcag gccatttacg cggcaaaatt ccacacgtaa tcctggtata agcacttctg
    49021 cgtcgcgggg agtgaatgcg gaaatatgga cctgaacttc tttacgaccg tcggagggga
    49081 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
    49141 acaaacaaat tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc
    49201 cagcgatatt taagaccgcc ggacggctaa aataaaattt gcttaatatc aattatcatg
    49261 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
    49321 tctcattatc aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc
    49381 tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa
    49441 aatgactggc ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
    49501 tgatggcagc aaagacgttt tcgtccattt cactgccatc cagagcaatg aattccgcac
    49561 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
    49621 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
    49681 catgttgtca tggttcacag taccgcacat cggcattcga tgtgacggag cgaaatcctt
    49741 tgggcgctaa gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc
    49801 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcg
//
LOCUS       DAJTMK010000008        48809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTMK010000008 DAJTMK010000000
VERSION     DAJTMK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48809
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.008"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..156)
                     /locus_tag="OO135_000390"
     CDS             complement(<1..156)
                     /locus_tag="OO135_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5396356.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVRMPY
                     SDEENSKM"
     gene            complement(282..587)
                     /locus_tag="OO135_000391"
     CDS             complement(282..587)
                     /locus_tag="OO135_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5396357.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(684..760)
                     /locus_tag="OO135_000392"
     tRNA            complement(684..760)
                     /locus_tag="OO135_000392"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(724..726),aa:Val,seq:gac)
     gene            complement(765..841)
                     /locus_tag="OO135_000393"
     tRNA            complement(765..841)
                     /locus_tag="OO135_000393"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(805..807),aa:Val,seq:gac)
     gene            1149..2405
                     /locus_tag="OO135_000394"
     CDS             1149..2405
                     /locus_tag="OO135_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5396358.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(2446..3819)
                     /gene="mdtK"
                     /locus_tag="OO135_000395"
     CDS             complement(2446..3819)
                     /gene="mdtK"
                     /locus_tag="OO135_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5396359.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAAGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            4034..4675
                     /gene="ribE"
                     /locus_tag="OO135_000396"
     CDS             4034..4675
                     /gene="ribE"
                     /locus_tag="OO135_000396"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5396360.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(4715..5863)
                     /gene="cfa"
                     /locus_tag="OO135_000397"
     CDS             complement(4715..5863)
                     /gene="cfa"
                     /locus_tag="OO135_000397"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5396361.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSDPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(6154..7365)
                     /locus_tag="OO135_000398"
     CDS             complement(6154..7365)
                     /locus_tag="OO135_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5396362.1"
                     /translation="MQPGKGFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAVQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRANVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRSEEAGCQNH
                     GNAEVAHSESH"
     gene            7478..8410
                     /locus_tag="OO135_000399"
     CDS             7478..8410
                     /locus_tag="OO135_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5396363.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(8407..9432)
                     /gene="purR"
                     /locus_tag="OO135_000400"
     CDS             complement(8407..9432)
                     /gene="purR"
                     /locus_tag="OO135_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5396364.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDSVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            9731..9820
                     /gene="ynhF"
                     /locus_tag="OO135_000401"
     CDS             9731..9820
                     /gene="ynhF"
                     /locus_tag="OO135_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5396365.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            9986..11155
                     /locus_tag="OO135_000402"
     CDS             9986..11155
                     /locus_tag="OO135_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5396366.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11301..11882)
                     /gene="sodB"
                     /locus_tag="OO135_000403"
     CDS             complement(11301..11882)
                     /gene="sodB"
                     /locus_tag="OO135_000403"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5396367.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(12010..12825)
                     /gene="mepH"
                     /locus_tag="OO135_000404"
     CDS             complement(12010..12825)
                     /gene="mepH"
                     /locus_tag="OO135_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5396368.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            13159..13506
                     /gene="grxD"
                     /locus_tag="OO135_000405"
     CDS             13159..13506
                     /gene="grxD"
                     /locus_tag="OO135_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5396369.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(13557..17905)
                     /locus_tag="OO135_000406"
                     /pseudo
     CDS             complement(13557..17905)
                     /locus_tag="OO135_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(17998..18645)
                     /gene="rnt"
                     /locus_tag="OO135_000407"
     CDS             complement(17998..18645)
                     /gene="rnt"
                     /locus_tag="OO135_000407"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5396370.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(18748..19155)
                     /gene="gloA"
                     /locus_tag="OO135_000408"
     CDS             complement(18748..19155)
                     /gene="gloA"
                     /locus_tag="OO135_000408"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5396371.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGSVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(19236..20333)
                     /gene="nemA"
                     /locus_tag="OO135_000409"
     CDS             complement(19236..20333)
                     /gene="nemA"
                     /locus_tag="OO135_000409"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5396372.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELLSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(20370..20969)
                     /gene="nemR"
                     /locus_tag="OO135_000410"
     CDS             complement(20370..20969)
                     /gene="nemR"
                     /locus_tag="OO135_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5396373.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            21072..21311
                     /locus_tag="OO135_000411"
     CDS             21072..21311
                     /locus_tag="OO135_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5396374.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            21360..22256
                     /locus_tag="OO135_000412"
     CDS             21360..22256
                     /locus_tag="OO135_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5396375.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            22337..22858
                     /gene="sodC"
                     /locus_tag="OO135_000413"
     CDS             22337..22858
                     /gene="sodC"
                     /locus_tag="OO135_000413"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5396376.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(22859..24870)
                     /locus_tag="OO135_000414"
                     /pseudo
     CDS             complement(22859..24870)
                     /locus_tag="OO135_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(24870..25727)
                     /locus_tag="OO135_000415"
     CDS             complement(24870..25727)
                     /locus_tag="OO135_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5396377.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(25793..25966)
                     /locus_tag="OO135_000416"
     CDS             complement(25793..25966)
                     /locus_tag="OO135_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5396378.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            26167..26601
                     /gene="slyA"
                     /locus_tag="OO135_000417"
     CDS             26167..26601
                     /gene="slyA"
                     /locus_tag="OO135_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5396379.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(26648..27115)
                     /gene="slyB"
                     /locus_tag="OO135_000418"
     CDS             complement(26648..27115)
                     /gene="slyB"
                     /locus_tag="OO135_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5396380.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            27389..28498
                     /gene="anmK"
                     /locus_tag="OO135_000419"
     CDS             27389..28498
                     /gene="anmK"
                     /locus_tag="OO135_000419"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5396381.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            28596..28925
                     /gene="mliC"
                     /locus_tag="OO135_000420"
     CDS             28596..28925
                     /gene="mliC"
                     /locus_tag="OO135_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5396382.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            28984..29640
                     /gene="pdxH"
                     /locus_tag="OO135_000421"
     CDS             28984..29640
                     /gene="pdxH"
                     /locus_tag="OO135_000421"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5396383.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLSLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDVWKIDRLAP
                     "
     gene            29769..31043
                     /gene="tyrS"
                     /locus_tag="OO135_000422"
     CDS             29769..31043
                     /gene="tyrS"
                     /locus_tag="OO135_000422"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5396384.1"
                     /translation="MASSNLIKQLQERGLVAQMTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            31102..31965
                     /gene="pdxY"
                     /locus_tag="OO135_000423"
     CDS             31102..31965
                     /gene="pdxY"
                     /locus_tag="OO135_000423"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5396385.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(32009..32614)
                     /gene="gstA"
                     /locus_tag="OO135_000424"
     CDS             complement(32009..32614)
                     /gene="gstA"
                     /locus_tag="OO135_000424"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5396386.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDDTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(32720..34222)
                     /gene="dtpA"
                     /locus_tag="OO135_000425"
     CDS             complement(32720..34222)
                     /gene="dtpA"
                     /locus_tag="OO135_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5396387.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(34833..35468)
                     /gene="nth"
                     /locus_tag="OO135_000426"
     CDS             complement(34833..35468)
                     /gene="nth"
                     /locus_tag="OO135_000426"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5396388.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(35468..36163)
                     /gene="rsxE"
                     /locus_tag="OO135_000427"
     CDS             complement(35468..36163)
                     /gene="rsxE"
                     /locus_tag="OO135_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5396389.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(36167..36787)
                     /gene="rsxG"
                     /locus_tag="OO135_000428"
     CDS             complement(36167..36787)
                     /gene="rsxG"
                     /locus_tag="OO135_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5396390.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(36791..37849)
                     /gene="rsxD"
                     /locus_tag="OO135_000429"
     CDS             complement(36791..37849)
                     /gene="rsxD"
                     /locus_tag="OO135_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5396391.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGS
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(37850..39976)
                     /gene="rsxC"
                     /locus_tag="OO135_000430"
     CDS             complement(37850..39976)
                     /gene="rsxC"
                     /locus_tag="OO135_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5396392.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAVPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(39969..40547)
                     /gene="rsxB"
                     /locus_tag="OO135_000431"
     CDS             complement(39969..40547)
                     /gene="rsxB"
                     /locus_tag="OO135_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5396393.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(40547..41128)
                     /gene="rsxA"
                     /locus_tag="OO135_000432"
     CDS             complement(40547..41128)
                     /gene="rsxA"
                     /locus_tag="OO135_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5396394.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDKWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(41205..41645)
                     /locus_tag="OO135_000433"
     CDS             complement(41205..41645)
                     /locus_tag="OO135_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5396395.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(41731..41946)
                     /gene="ydgT"
                     /locus_tag="OO135_000434"
     CDS             complement(41731..41946)
                     /gene="ydgT"
                     /locus_tag="OO135_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5396396.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(42219..42344)
                     /gene="blr"
                     /locus_tag="OO135_000435"
     CDS             complement(42219..42344)
                     /gene="blr"
                     /locus_tag="OO135_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5396397.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            42587..43627
                     /locus_tag="OO135_000436"
     CDS             42587..43627
                     /locus_tag="OO135_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5396398.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(43662..44663)
                     /gene="add"
                     /locus_tag="OO135_000437"
     CDS             complement(43662..44663)
                     /gene="add"
                     /locus_tag="OO135_000437"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5396399.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(44767..45939)
                     /gene="malY"
                     /locus_tag="OO135_000438"
     CDS             complement(44767..45939)
                     /gene="malY"
                     /locus_tag="OO135_000438"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5396400.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQERVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(45949..47535)
                     /gene="malX"
                     /locus_tag="OO135_000439"
     CDS             complement(45949..47535)
                     /gene="malX"
                     /locus_tag="OO135_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5396401.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVSIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHGLS
                     TKWYMVSVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGYNV
                     PAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQVVI
                     GPQVQSVKDEMAGLMHTVQA"
     gene            47710..48738
                     /gene="malI"
                     /locus_tag="OO135_000440"
     CDS             47710..48738
                     /gene="malI"
                     /locus_tag="OO135_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5396402.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSQVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     CKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT        12355 a        12280 c        12680 g        11494 t
ORIGIN      
        1 catttttgag ttttcctcat cactgtatgg catcctgaca ccggcctcgt cagcctgctc
       61 gaagatagaa atagaaagtg gagtttgttg ctgtagaagc gagaataagg tgtagatccc
      121 cgtaggcccg gcacccacaa tagcaatttt tttcattgtt acttacctgt acgcattctt
      181 tcgaatttag tgttatcaga taagtttggt tgagattttt agaatttgag aaatttcaga
      241 cagggatgaa aagggcgcta accgcccttg attaaattct gtcaggcgag ttttgcctga
      301 ttgatttgac tcagtggctc attgacatcg aaaactttgg cgacaacttc ctcaacgccg
      361 agatttttaa gtcgggcagt atgtttttcc agataggcaa gggcgctttt ttcatcagta
      421 aaaagataga tcccaccggc ttcgtggttc ttttcacttt ctgtccatac cttccacaga
      481 aaaccaggtt cctgattaat cgattcagca agcggtttaa gctgctcagc cattgcgtcg
      541 ccaaatgggc cattaaaagc aaaatgaagt tgtaacaaag ttgccatagc gattctcctt
      601 ctgataacgc atacgaagac agtaacgtat tggatacaga gaagaaacag agggaaaaag
      661 gagaagacca gattaagaaa atctggtgcg tccgagtgga ctcgaaccac cgacccccac
      721 catgtcaagg tggtgctcta accaactgag ctacggacgc aggatggtgc gttcaattgg
      781 actcgaacca acgaccccca ccatgtcaag gtggtgctct aaccaactga gctatgaacg
      841 caacgttgta ggtgacaacg gggacgaata ttagcggcag agtgggaagg tggcaagagg
      901 caaaacgtaa ttttctgcgc tatttcgacc gtttgcagag cttttaagca aattggctat
      961 attttgttga tttgcaaggg cgatttttat tcaggatcgc atttacatct gatgcaaccc
     1021 aataattcgt gtatcagagt ttaaacagag ttgtttgcca ccatctcaaa tctgtcgtgg
     1081 ataaattctc cgtagccttt acacttaggt agcaataaag gcacacagat gcagcaaagg
     1141 aataaatcat gggatctgat gcgaaaaact tgatgagcga cgggaatgtg caaattgtta
     1201 agaccggcga ggtcattggc gcgacgcaac ttactgaagg cgagttgatt gttgaagctg
     1261 gcggaagagc cgaaaatacc gtggtcacgg gggctggctg gttgaaagtg gcaaccggtg
     1321 ggatcgccaa atgcacacag tacggtaaca atggcacgct atcggtcagc gacggtgcca
     1381 ttgccacaga tattgttcag tccgagggag gcgcaattag tctctctacg ctcgctacgg
     1441 ttaatggccg ccatcccgaa ggtgaattca gcgttgatca gggttatgcc tgcggtttgt
     1501 tgctggaaaa tggcggtaac ctgcgtgtac tggaaggaca tcgcgcggaa aaaatcattc
     1561 tcgatcaaga gggcggcctg ttggttaatg ggacaacctc agcggtcgtg gtagatgaag
     1621 gtggtgaatt gttggtgtat ccaggtgggg aagccagcaa ttgtgagatt aatcagggcg
     1681 gcgtttttat gctggccggg aaagccagtg atacgttgct tgctggtggc accatgaata
     1741 atctcggtgg tgaagactct gacactattg ttgagaatgg atccatttat cgtctgggga
     1801 cggatggcct tcagctctac agttccggta agacgcaaaa cctgtccgtg aatgtgggtg
     1861 gtcgggctga agtgcatgcc ggtacgctgg aaaatgcggt aatacaaggt ggaacagtga
     1921 tcctgttgtc acccaccagc gcggacgaaa attttgtcgt agaggaagat cgcgcaccgg
     1981 ttgaactgac cgggagtgtt gcattactgg acggcgcttc aatgattatt ggctatggcg
     2041 cagatctgca acaatcaacg attactgtac agcagggcgg tgtgttgatt ctcgacggca
     2101 gtacggtaaa aggtgacggt gtcactttta ttgttggtaa catcaatctg aatggcggaa
     2161 aactgtggct gatcactggt gcggcaacgc atgtgcaact gaaagtgaaa cgcctgcgcg
     2221 gagagggagc gatttgcctg caaaccagtg cgaaagaaat ctcacctgac ttcatcaatg
     2281 tgaaagggga agttaccggt gatatacacg ttgagataac agatgccagt cggcaaactc
     2341 tgtgcaacgc tctgaaatta cagccagacg aagacgggat tggcgcaacg ctccagcctg
     2401 cgtaaaaaca caggcgcaac ccgtgaaggt tgtgccttgt ctttattagc gggatgctcg
     2461 ttgcagaatg ataactgacg gcagacgttg caggaaccgc atacgcaaca tcatcataat
     2521 ggctgccgat gtcaggccga taataaagcc tatccagaag cctgctggcc ccataggttc
     2581 aacgaccaga tcggtcagtg ccagaatata gccgcttggc aagcccagca cccagtaagc
     2641 tgtaaaggtg atatagaaaa tggaacgcgt atctttataa ccacgcaaaa tcccactgcc
     2701 aatcacctgg attgagtcag aaatctgata taccgccgcc agcaacatca aatgcgcagc
     2761 cagcgttaca acctcgggat tgtcgttgta caacagggcg atttgctccc ggagtgaaac
     2821 cgtgaaaatg gccgtcaggg ttgccataca gacacccacc ataagcccgg tccgcgcagc
     2881 ggtttgcgca tccagcgttg agccctgacc cagacgataa cctacgcgga tagttaccgc
     2941 tgccgccagc gacattggaa gcacaaacat cagtgaacta aagttcaggg caatctggtg
     3001 tcctgcgaca tcaacaatac cgagcggaga cactaacaga gccacgacgg caaacagtgt
     3061 cacttcaaag aatagtgcca gcgcaatcgg taaaccgagt tgaatcagtc gtttcataac
     3121 cgcaggatcg ggtttcgcgg tgcctttttc gttacgaata tcgcgcatgg agcgggcgcg
     3181 tttaatgtaa gaaaccatgg caaggaacat gacccaatac accgccgcag tagccacgcc
     3241 acaaccaacg ccaccgagct caggcatacc gaaatgacca taaataaaga tatagttcac
     3301 cggaatgttc accagcaggc cgataaagcc cattaccata cccggcttgg tttttgccag
     3361 accttcacat tggttacggg caacctggaa gaacagatag cccggcgcgc cccacaacaa
     3421 cgcacgcaga taacccgcgg ctttgtccgc cagagccgga tcgatgtttt ccatggagcg
     3481 gataatgtaa cctgcattcc acagcaccag cataatgaga acggaaacaa aacctgccag
     3541 ccagaaacct tgtcgcacct gatgcgcaat gcgctcacgt cgaccggaac catttaattg
     3601 cgcgataacc ggcgttaatg ccagcagcag tccgtgacca aagaggatcg ccggaagcca
     3661 gatagaagta ccgatagcga ccgccgccat gtcggtggca ctatagccgc ccgccatcac
     3721 ggtatcgaca aaacccatcg cagtttgggc gatttgcgcg agaatcaccg ggattgctaa
     3781 tgctaataac agacgcgctt cactgatata cttctgcacg tgaacacctt ttatttgtag
     3841 ttatatgaga gactaaaaag ccgcctgaat gggcagcaaa aagaagaagc aggggaaatt
     3901 cagtctattg tagcgaggat ttattaattc tccagtgaaa aaatagtcat tcttacccct
     3961 tcactggtaa gggcgtttgc cacctgctat tgtgctgaac agaatgtctt aactgatttc
     4021 aggagttgta agtatgttta cggggattgt acagggcacc gcaaaactgg tgtcgattga
     4081 cgagaaacca aattttcgta cgcatgtggt ggagttaccc gaccacatgc tggacggcct
     4141 ggaaaccggt gcttccgtgg cgcataacgg ttgctgcctg accgtgacgg aaattaacgg
     4201 caaccatgtc agttttgacc tgatgaaaga aacgttacgc attaccaatc ttggcgattt
     4261 aaaagtgggg gattgggtaa acgttgagcg tgcggcgaaa ttcagtgatg aaattggcgg
     4321 acacttaatg tcaggtcata ttatgaccac cgctgaagtg gcgaaaatat taacctcgga
     4381 aaataatcgc cagatctggt ttaaagtcca ggatagtcag ttgatgaaat atatcctgta
     4441 caaaggattt attggcatcg acggtattag cctgaccgtc ggcgaagtca cgccaacgcg
     4501 tttttgcgtc catttaattc cggaaactct ggaacgcacg actcttggga agaaaaaact
     4561 cggcgcacgc gtcaacattg aaattgatcc acaaactcag gcagtggtag atacggtaga
     4621 acgtgtgctg gcggcacgag aaaatgccat gaatcaacca ggtacagaag cctgatctga
     4681 aatcgccccc ggagaggggg cgatagaata gcctttagcg agcgactcga aggccgtttt
     4741 caacaccgcg tgagaacacg acctgccaga gctgaatatc acgggcgcgg aaagcacctg
     4801 cgcaggcatt cagataatag gtaaacatcc gtttaaagcg ttcactgtaa ttatccgcaa
     4861 tttctggcca tgcggcgagg aatcgttcat accacgccat caacgtagta tcgtaatcag
     4921 caccgaagtt atgccagtct tccatcacaa agtgggggtc gctggactga gcaatctggc
     4981 gtacagaggg caggcaaccg ttcggaaaaa tatatttatt aatccaggga tcaacattca
     5041 gatcggtttt tttcgaaccg atagtatgga gcaggaatat gccttccggt ttcaaattac
     5101 gatccaccac cgcaaaatag gtatcgtaat ttttcggtcc gacgtgctcg aacatcccca
     5161 cagaaacaat acgatcaaac tggtcgttca ggtcacgata atcttgcagc aaaatggtga
     5221 catccaggcc ttcacagcgt tcctgagcca ttttttgctg ttcggcagaa atggtgacgc
     5281 ccaccatgct tacgtcataa ttagatgcca tgtagtgtgc cagtccgccc cagccgcagc
     5341 caatatccag tacgcgcatc cctggtttta actgcaattt ttcacaaatc attttgagct
     5401 tcgcctgctg ggcagattcc agattatcgg catctttcca gtaagcgcag gaatattgca
     5461 tgaagggatc aagcatgcgg ctgaacaagt cattacccaa atcgtaatgc tctttgccga
     5521 ctatccaggc acgtttttta ctctgcagat tgaagagacg agcgccggca atacgcagcg
     5581 tgtctttgaa atgatggggg agttggttct cgagacctgc gcgtaagact ttgctaaaaa
     5641 acatatccag tcggtcacat tcccaccagc catccatata actttcgcct aaccccaaag
     5701 agccttcttg cagaacgcgt ttaaaaaaat cggggttttt cacacgaata tccgccgggg
     5761 cagaaccgtt aatggctata ccggcacggc taagtaattc gttggcgata cggtaccagt
     5821 tgtcatccgg tacgctgact tcttctatac acgatgaact catagtttct ccatcactgg
     5881 tcggtgatca gaaccttaaa acagcgtaga cgctttttgg gctttgtgag aaatctcgca
     5941 gaaaaaaccg cgagtctata atccgacgta gaacagaggg aaaggaggaa cccttgccga
     6001 aatgaccatc catagtgagt cgggaacgat cctgtccccg taaaaacata atatttatcg
     6061 tagctttaac aactcgaaat tagtataggc cgctaatttc aatcatacaa caaaattttg
     6121 ttagcagcct aagtataagt atatcgatat agatcagtgt gattcgctat gagcgacttc
     6181 ggcattgcca tgattctggc agccagcttc ttcactacgt tgcatcatgt aacccagcgc
     6241 gaccagcact actgttgata acatcacgct ggtggtggtg agcaatggcg tgctgatact
     6301 aataagccag gaaactacca gacttgcgag gaagcacaga cccagttgta gagtgttctg
     6361 caacgctgcg gcgcgaccag ttgcgtgtgg gaagggacgc agcgcctggg cgacaacaat
     6421 agggtagatc gcgccattgg caatcgccat cacacagaat gggatcagga tttcgaccag
     6481 cgacacatgg ctaatgaagc ccgcagccca ggtcgcaatg acgctgacag caaacagcac
     6541 cagcaaccac ggtaataact gcttgccttg ccatttctgc agcgcggcgc gacagccata
     6601 accaccaatc agaaacgcga tagtttgcgg gacataactt aaaccaataa ctgccgggct
     6661 gtagcccatt tcactaagga tgaacggtga accggtcagc catgcaaaaa aactggctga
     6721 acaggccgcg tatatcagca cgttggcgcg ataggtttta gaacgtagca ggtcggtaaa
     6781 ggtcagacca tcctgactat tgttaccggc cttcgtcgtg ggtttgagcc agaaaatagg
     6841 cagaatcagc accacggtaa tggcaaacag ggtggcgaaa atcgcctgcc aggaaaaatg
     6901 gaccagcagc cagcttccta acagaggagc cagggccgga gatagaccca ccagcggcat
     6961 gatggtcgca aaaattcggt taactttctg tgaaggataa taatccgtca ctaacgcttg
     7021 ccagataacc gccgcggcgc agacacccac agcctgtaca aaacgcaata ccagcagcgt
     7081 agcggcgttt tctacccaca gcatccccag actacctaac gcaaaaattg tcaggccgat
     7141 taataacacc ggtttacgac cataacggtc ggagagtggc ccccacagaa gctggactgc
     7201 ggcaaaaccg gcaaggaaca gactaaggct ggcactgaca gcagacgcag gcgtttgcag
     7261 gtcggcctgt atggcggcga aagcaggcag atacatatcg gttgccagaa aaccgagtac
     7321 gctcaaaccc gccagccaga ctaaaaatcc tttcccaggt tgcatgtata ttctctaaat
     7381 ctctcaatta aacaccgccg caaagtgtag ggagtgaaat tagtgttgtg aaacggtaat
     7441 atttgatgac tgatttcaaa aattttgcag gcagactatg tggtcagaat attcactcga
     7501 agttgttgat gcggtagcgc gtaatggtag ttttagcgct gcggcacagg agctgcatcg
     7561 cgttccttct gcggtcagct ataccgtgcg tcagctggaa gagtggctgg cggtgccgct
     7621 ctttgaacga cggcaccgtg atgtggagct gaccgctgct ggagcgtggt ttctcaaaga
     7681 agggcgctct gttgtcaaaa aaatgcagat cacccgccag caatgtcagc agatagcgaa
     7741 cggctggcgc ggtcagttag ctatcgcagt ggataatatt gtcaggccag aacgtacacg
     7801 gcagatgatc gttgattttt atcgccattt tgatgatgtc gaacttcttg tctttcagga
     7861 agtgttcaac ggtgtctggg atgcgctttc cgacgggcgc gtggaactgg cgattggcgc
     7921 aacacgcgcg attccggtag gcggtcgtta tgccttccgg gatatgggga tgctaagctg
     7981 gagttgcgtt gttgccagcc accacccgct ggcgttgatg gatggcccat tcagcgatga
     8041 tacgttgcgc aactggccgt cgttggtgcg cgaagacacc tcgcgaacgc tacctaaacg
     8101 tattacctgg ttgctggata accaaaaaag agttgtcgtg cctgactggg aatcatcggc
     8161 aacctgtatc tcggcaggat tatgcatagg gatggtgcca acacatttcg ccaaaccgtg
     8221 gcttaatgag gggaagtggg tagcacttga attggaaaac cccttcccgg attcggcatg
     8281 ttgtctgaca tggcagcaaa atgatatgtc gcctgcgtta acctggctgc ttgagtatct
     8341 cggcgatagt gagacgctga ataaggagtg gctgcgggag ccggaagaga ctcccgcaac
     8401 gggtgattaa cgacgatagt cgcggaacgg gccgtcagcc acggagcggc gttcaatcaa
     8461 gcgcggatgc acttcaatgg actgcggttc ttcacgtttg ttgacgatac gatccaacag
     8521 catgttgaac gctgtttcac ccagcgaatc ttttggctgg tggatcgtgg tcagcgccgg
     8581 cgtaaaatag cgggcgttgc gcacgttatc ataaccgatc agcgaaacat cctgcgggac
     8641 gcgcagaccc atttcatcag cagcacaaag tgcgcccatt gccatgatat cgccaccaca
     8701 gaagacggca gtagggcgat gcggctgcga cagtatttgc tgcatggcgc gataaccaga
     8761 ttcgggttca aagtcaccct gaacaatcca gctttccggc accttgatca tcgcttcttc
     8821 catcgccttc ataaaaccgg caaggcggcc tgcgccggtg ttacgttcca gcgggccggg
     8881 gatgacgccg atttcgcggt gaccgcgttc aatcagataa cgcccggcca tgtagccgcc
     8941 ttcgaacgcg ttatcaatga ccgaatcggt gaagtcagct tttgcttcac cccagtccat
     9001 caccaccatt gggatatggc gatactcttc cagcatcgcc agcaacggct ctgggtactc
     9061 agaacacatc accagcagac catcgacgcg tttttgcgcc atcatcgaca gataagcccg
     9121 ctgtttctca agattgttcc acgcattacc cagaatcagg gtgtaacctt tctggaagca
     9181 atttttttca accgcttcaa tgatctcggc aaaataggcc gcttcgctgc tggtcgccag
     9241 caaaccgata gacttggtgt ggttaacctt caggctacgc gccaccgcgc taggggagta
     9301 gtgtaattct ttaatcgctg cccacacggc gttgcgcgtt tcttcagcga cgaaacgtgt
     9361 tttgttgatt acgtgtgaca cagttgtagt ggaaacgttt gctcgtttcg ctacatcttt
     9421 tattgttgcc attccatttc actccagacc ctaacttcag ctcctgcaaa atcgcaaggt
     9481 aaacgtttgc ctttacacac cttacgcaca acaaaagtgt tgcggtacgc cggaaaaacg
     9541 acacggagcg tcaggaaggg gtcaatggcc agtacgcaaa tagtgtaagc gtggaatttt
     9601 ggccgatctt gacgaaaagg ggaagtggta aaaacactta tcattataaa tgacgggaga
     9661 ttttttcatc acagtgtgta aaaatgcgta atatcactat ttgtgtggta ataaaaaagg
     9721 agaaaacttg atgagcaccg accttaaatt ttcactggta acaacgatta tcgtcctcgg
     9781 tttgatcgtg gccgtgggtt tgactgccgc gctgcactga tttcctctcg agggagcgcg
     9841 ttctccctcg catctccttc ctgtttgtta gcaaaatggc aaaagtgttt tctctttttg
     9901 ttattttcat ttttttgtga ttgctgtcat gcttttgcca ctttaatgaa tatcgcgtaa
     9961 tgacgcgaag aggtggagat aaggcatgaa aattaactat ccgttgctgg cgctggcgat
    10021 tggcgcgttt ggtatcggga caacggagtt ctcgccaatg ggcttgttgc ccgtcattgc
    10081 gcgcggtgtg gatgtctcga ttcccgctgc cggaatgtta atcagtgcct atgcagttgg
    10141 cgtaatggtt ggcgcgccgc tgatgacgct tctactttct catcgtgccc gccgcagtgc
    10201 gttgattttc ctgatggcaa ttttcacgct cggcaacgta ctttccgcca tcgcgccgga
    10261 ttatatgacc ctgatgcttt cacgcatttt gaccagcctg aatcacggag cattttttgg
    10321 tttgggttca gtcgtggccg caagcgtgga gccaaaacat aaacaggcca gcgcagttgc
    10381 cactatgttt atggggttaa ccctggcaaa tatcggtggc gtgccggcgg cgacctggtt
    10441 gggtgaaacc atcggctggc ggatgtcatt tctggcaacg gcggggctgg gagtgatttc
    10501 aatggtaagt ctgttcttct cattacctaa aggtggtgca ggggcacgac ctgaagtgaa
    10561 aaaagagctg gcggtattaa tgcgtccgca ggtgctgtct gcattgctga cgacggtact
    10621 gggagctggt gcaatgttta ctctctacac ctatatctct ccggtactgc aaagtattac
    10681 ccacgcaaca ccggtgttcg tcacggcaat gctggtgctg attggtgtcg gattctctat
    10741 cggtaactat cccggcggca aactggcaga tcgttcagtt aacggcacgt tgaaaggctt
    10801 tttgttgttg ttgatggtga ttatgctggc aatcccgttc ctggcccgca atgagttcgg
    10861 cgcagctatt agcatggtgg tgtggggcgc agcaaccttt gcggtcgtac cgccgttaca
    10921 gatgcgcgtg atgcgtgtcg ccagtgaagc gccaggtctg tcttcatcag tcaatattgg
    10981 tgcctttaat cttggaaatg cgctgggagc agctgctggt ggtgcggtaa tttccgctgg
    11041 gctgggatac agctttgtgc cggtgatggg agcgattgtc gcgggactgg cattattgct
    11101 ggtgtttatg tcagccagaa aacaacctga aacagtttgc gttgctaaca gctaattaaa
    11161 caaaaacgca gaaggataac cttctgcgtt acagttgctg acaacgtgcg atttatcttg
    11221 ccgaatgcgc tgttaacgcc tcatccgcgt tggcatggta aacgaagcgc atcaggcaat
    11281 gctgcatttg ccataagtta ttatgcagcg agatttttcg ctacgaattc ccagttcacc
    11341 agcgcccaga agtgctccag atagccagga cgtgcattgc gatagtcgat gtaataagcg
    11401 tgttcccaga catcaacggt cagcagcgga gtcgcatcgg tggtcagcgg agtacccgcg
    11461 ttagaggttg aaacgatagc cagtttgcca tcgctgtttt tcaccagcca ggtccagcca
    11521 gaaccaaagt ttttgatcgc tgcatcagta aactgcgctt tgaaatcggc aaagctgcca
    11581 aaagatgcgg cgatagcttc agcgactttt ccagtcggtt cgccaccggc gttcggtgcc
    11641 aggcagttcc agtagaaagt atggttccag acctgagctg cgttgttgaa tacgccacct
    11701 tcagagctgc gaataatctc ttccagtgat ttaccttcaa acgcggtacc tttaatcagg
    11761 ttgttcaggt tagtgacata agtctgatga tgcttgccgt agtgatactc gatagtttcc
    11821 gcagaaatgt gcggtgccag agcatcttta gcatatggta gtgcaggtaa ttcgaatgac
    11881 attgctactc tcctttatta ttaatttgca tacgtacaat agccttattg tgcgtatgga
    11941 tagggtagca aattaaaagg tgagacaaaa gatgaactta ccctgttgcc gtaacaacag
    12001 ggtaaagttt tagcgaagtg tttttggggt cattacccga cgagcgccaa catagtggcg
    12061 ctgccagtag tcttcactga gagaagtgat ttgaatttcc tgacctgtgc gcggtgactg
    12121 aataaatttg ccgttgccga catacacgcc gacatgatcg gctgtgccgc gtccctgagt
    12181 acggaaaaag accaggtcgc cgtttttcag ttcactacgt tcgattggcg ctgcatcacg
    12241 caggtgatac atttcattcg ccgtacgcgg aatacgaatt ttcaccaaat ctttataagc
    12301 gtaataaacc aggccgctgc aatcaaaacc ggtacgcggt gagctgccac cccaacgata
    12361 tggcttacca atttgctgca tcagtttatt cattgccact ttcgtagctt tttgcacctt
    12421 cgctttgtgc gcgtcggcaa gagttcctga cgcggcattt ttgactttca cacaatgcga
    12481 tttatagccc ttacgtttgg tacacttttc ggtaaagctg gctgaggccg ttttattgac
    12541 ggcgtgtttg ctgcgagttt tggcgttttt agaagcggta atggaagatt ttttaacgga
    12601 agaagcggtt ttgcttttgg tcgtactttt actggcggct tttttcgctg ttttctgggt
    12661 ctttttggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat gagtggtagc
    12721 agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca gggtggtaaa
    12781 aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc gtccctctgt
    12841 taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct aatgcataga
    12901 agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgttggcg agcgcaaaaa
    12961 aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccggc atcagagaag
    13021 aaacgaatat ttgaaatgca acaaatagag caaagggata aaatagcaaa agcgtgatga
    13081 aaatgttatt ttccgatgcc gcactgaccc gctacaatgt cagattactg ccgtaaagaa
    13141 gtttgaggaa gcaagacaat gagcaccact atcgaaaaaa tccaacgcca gattgctgaa
    13201 aacccgatcc tgctgtacat gaaaggttca ccgaaactgc cgagctgcgg tttctctgcc
    13261 caggcagtcc aggcgcttgc cgcatgtggc gaacgttttg cctatgttga tattctgcag
    13321 aatccggaca ttcgtgcgga actgccgaaa tatgctaact ggccgacctt cccgcaactg
    13381 tgggttgacg gcgagctggt cggcggttgt gatatcgtga tcgaaatgta tcagcgtggc
    13441 gaactgcagc agctgatcaa agaaactgcc gctaaataca agtctgaaga gccggacgcg
    13501 gaataatccg tcctgcaata ctgacggatg cgaacgggca tccgtcagtc ctttctctat
    13561 ccccaatcca gcccttgtgg cgaacttgaa aatcccgcct catgcagcag cgtaaacatc
    13621 ggcgtttgcc ggaccggtag atcattcact tctgttagcg taaagcgcag ccgtggttcg
    13681 cgacgcagtg cggtagtcag cgcgtggaaa acctctgggg cgagtaattc ctctttttcc
    13741 tgccagacca gcattttttt gccaccttgc gccagataga gtaacaattt gccgctagaa
    13801 acgaccacca gcgcacccgc ccgacgcgtt ggaaccagcg ttgccggatg tgcaggccag
    13861 ggcagaagat ttccccacac attagccgga tcgttggcag agagcgccac tggtgtatag
    13921 tggcgcgttt gcgtggcttg tgtcgccaga tcgcgccatc ggtcaatagt cagacgttca
    13981 gcgaattgcg cgccacccag accttctaca aaacgacctc gcataattcg cccggagtct
    14041 tccatacttc gacaaagcgt ttgcatcgat ggaaacccgc cagggatatt ttcggctatc
    14101 accgcctgac gactgatgat gccgtagcag tcgagcatgt tttccgccag cgccagcatc
    14161 ctttcggtat cgtttagtgg ctccacctgc aataacgacc agcgtccagc cagatttggt
    14221 gtgttgtaag ataccagcgc gagacgggtt gcgcatagac aggacgtcca cgacgagccc
    14281 ggtgactgcg gcgagttgag gtgcgtgcgt tggaactgct gcgggtgagg gcgcgtaacg
    14341 gtgcccaaat gtcgctggtg atgacgcctt gccataccag cgcccataac gcctcttgca
    14401 gggcagagag atcaaccgat tcgccaattt tgtcgcgtat ccgttggctg atttgttgtg
    14461 caaaccaggc tcctccgtca gccagaacag cgactatcgc ttgttgcagc gccgaacgat
    14521 tcgcctgatc cgcttcggcg ggagtgaacg attctgcagc atattcctgt agatgcagtg
    14581 ccaccaggcc gtcatcttca cccagttttt tttgccccga ccagataacc gcaccggttg
    14641 ccagtaattc atcgagcatt tctgacgaat agtcgcgtac gcgggcaggc aggatctggc
    14701 tttcccagag tgaggcgggt aatacgccct gacgttccag cagcaatcgc gcataggtcg
    14761 tggctgcaac gggacgcgtc gcttctctgg cggcttgcag cgagcgcaaa cgcagacgac
    14821 gaaatacttc atcgctcacc cagatgtctt gttgcagatt catcaccaga ccctgttcac
    14881 gcagttgctg aagctgctct tcgacaatgg caataccgag actaaactca tgagccagtt
    14941 gttcagccgt gaccagagca tgagcccgga gatagcgcag aaagaggtcg cgaagcgggt
    15001 aactgactct atgtaaataa atctctggca atgactctgg tagtcgtacg ccgagggcat
    15061 cacgcagcct ggcggcatca tccatacagg caagacgctc ctgtccgcta atcatcgctg
    15121 ggaagattcg ttttactgca agaagatttt ccagatacga cgcaacctct tcactgctgc
    15181 ctgtatgctg ttgcgccagg tcttcaacgg tcattggccc cagttcgcgc agcaggtcga
    15241 acaatccttc ttcacctttc gctcttctgc caggagccag tcgttgcaac tcttcttcca
    15301 cctggcgaat gacctgcggg tcgagtaatt cccccggatc gacctgtccg agtagattgc
    15361 gcagtaactc gctgtccagc gacagtacgg atgcccggcg ctctgccagc ggggcgtcgc
    15421 tctggtacat aaattccgcg acatagccga acaataaact tgtggcgaaa ggcgagggcg
    15481 tagtggtcgt tacatcggat atttgaattt cgccaccgtt caggcgacgc atcaaacgtt
    15541 ccagtgcggg aagatcataa acatcttgca gacattcgcg taggatttcg agaatgaccg
    15601 gaaaatctgg atatccctga gcgatttcca gcaactgact ggcgcgcagc cgttgttgcc
    15661 aaagcggggt gcgatggccc ggagtgcgcc ccggcattaa taatgcccgc gcggcgcatt
    15721 cgcgaaaacg ggcggcgaaa agtgccgagc tgcctaccgc ctcgcggaca atttgcagca
    15781 acttttcggg ttcaaacaaa aaaatcgcgg cgtcgggcaa tttaccatca gtgtcaggaa
    15841 tgcgcgcaac aatgccgtca tcactggcga ccacagacgc gtcagcgccc catagggcat
    15901 gtattcgccc ggcaatcgcc agcgcccagg gttcatgcac ccgtcttcca tagggagagt
    15961 gcaaaataat acgccagtcg ccaatttcat cacggcaccg ttcgaggacc agatggcgac
    16021 tccccggaac gatgcccgtc gcgttgcgtt gctcgtcaat caacccctga atattggcgt
    16081 tcgtattttc ttctgccagc cacgggggaa tagtcccgga aaagaacgcg ccatccgcca
    16141 gtaaatgaag aaaatcgccg atcatctcgc ctaattcagc cggacgtccg ttaccttcac
    16201 cacgccagaa ggggagccgg gcagaacgac ccggagcagg agtcacaatc acctgatcgc
    16261 gggtgatttg ctggatccgc catgaggtag cgccgagagt gataatgtcg ttcacccgcg
    16321 actcatatac catctcctca tccagttcac ccacccgccg cgaaccggcc ttttcttcac
    16381 cttcgggtaa taacacgcta tacatgccac gatccggaat ggtgccgccg ctggtaaccg
    16441 ccgacaattg agcgccaggt cgggcggtca atatcccggt ctccctgttc cagaccagtt
    16501 tggggcgaaa agcagaaaaa tcgccagagg gatagcgccc ggaaagcata tccagcgtgg
    16561 cgtcaaaaac acgtcttggc agatctttcc acggtgcggc acggcgtacg cgggagtacc
    16621 attcgtctac ctgtaatgcc tccatcgccg cggcggcaac ggtttgctgc gcaaggacgt
    16681 cgagtggatt atgcggcggt gtcaggtttt ccagcctgcc tgcgaacata cactctacaa
    16741 tgactgcgga atcgactaag tcacgccggg tacgagggaa aaacagccct ttagatacac
    16801 cgccaacctg atgtccggcg cgaccaatgc gttgtaaccc actggcaaca gaaagcggcg
    16861 ttgccacctg aatcaccaga tccactgcgc ccatatcaat ccccagttcg agactggagg
    16921 ttgcgaccac gcagcgtaac tcacccgatt tcagcgcctg ctcggtgatt gctcgttgtt
    16981 ctttagagac ggagccgtgg tgtgagcggg caataaaaac gtcgctactt tgtacacggt
    17041 tagaggttgc gccggaggtc gactcgaaat gggccgcatc aacggcgata gacggggaac
    17101 gctgtaagcg tgcggcgtaa agctcattta atcgtgccgt cagtttttcc gccagcccac
    17161 gtgaattagt aaagacaatg gtcgagcgat ggcgcaacac ttcatcaagg atacccgttt
    17221 caatatatgg ccagatggag ccttcccggc cggcatggct gtcttcgccg gtgctgctgg
    17281 cgaccgatga gacatcatcc atattggcga ccggtacgac aattcgtatc tgcggatggc
    17341 gcattgcggg cgggttgact accgtaaccg ggcgatcgcc accaagaaag gctgccacat
    17401 cgctggctga gcgcacagtg gcagaaaggc caattcgctg tgctgaggtg tggagcagcg
    17461 catcgagccg ctccagactt aacgccagat gcgcaccacg tttactgccc gctaccgcgt
    17521 ggacttcatc aataattacc gtttcgccgc gccgccgccg ttcatcggca atacccttca
    17581 acgggagctg caagttgcgc tgaacgtcgg tgcccagagc ttttatcggt gaaatataga
    17641 ggatgcgtga ggttttacgc ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga
    17701 gccgatcgag ggcgtagaga aatgctgcca gcgttttccc ggagccggtc ggtgcaatca
    17761 ccagcgcatg ttcgcttcgc gccgccacat gccaggtttg cgactggaca gcggtcggct
    17821 gtttaaaggc gcgaagaaac cagtcgcggg tcgccggtga aaacacgtcc ggcaggagcg
    17881 atgaagggtc tggattatct gccattaacg ctattccccg tgaatatcat gcaggatgcg
    17941 tggaatagcc tcactataga tgcctgaatc attgcatgtc atcaggcatc gactcgatta
    18001 cacctcttcg gcggcaggta gcggccagcc tcccagacgt ttccagcggt tgacgatttc
    18061 acaaaacagc acagcagtgc gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc
    18121 gaagtccatg ccagcggtct ggcaagcctt tgacaatacg gtttgtccga gtgccagccc
    18181 ggccagtgca gcagtgtcaa aagtggcgaa agggtggaac gggttacgtt tcagtgaggc
    18241 gcgttctgcg gcggccatca taaagctgtg atcaaaattg gcattgtgcg ccaccataat
    18301 ggcgcggtta cagccgctcg ctttaatacc tttacgtaca actttaaaaa tttcgtgcag
    18361 cgcctcgtat tcgctgaccg cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc
    18421 gagggcttct ggttgcaaat ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt
    18481 gtccggcatc agccagcctt gttcatccat tttcagggtg atggcggcaa tctcaagcag
    18541 cgcatcggtt ttggcgttaa atccggctgt ttcaacatcg atcacaacag gataaaaacc
    18601 acgaaaacgg tcgcacagac cggtaagttg agcgttatcg gacatctgat tctcttaata
    18661 cggataaatt gcagcgcgca ttatgacaaa tattacttgt cgatgcagta aagatgcggg
    18721 cgcgatgagt tcacgcccgg caggagatta gttgcccaga ccgcgaccgg cgtctttctc
    18781 ttcgattaac tcaattttgt aaccgtccgg atcttccata aacgcgataa ccgtagtacc
    18841 gccttttacc ggacccgctt cacgggtcac gctacccccg ttttgacgga ttttttcgca
    18901 cgcttcagcg gcgttatcta cgctaagcgc gatgtgacca taagcagtgc cgagttcgta
    18961 tttatccacg ccccagttgt aggtcagttc aatcaccgct tcttcggttt ccgggccgta
    19021 gccaacaaac gccagtgagt atttgtattc cgggttttcg ctggtacgca gcagtttcat
    19081 gcccagcact ttggtataaa aatcgatgga gcgttgcaaa tcgccaacgc gcagcatggt
    19141 atgaagaaga cgcatctttt tatcctcaaa atggctaaca gattcaaaat gttgttttag
    19201 tatagcggcg gctttacgcc gctcgcaatg ttggattaca acgtcgggta atcggtatag
    19261 ccttccgcgc cgccaccgta gaaactttcg gcacgctgtg ggttaagctc agctttgcgc
    19321 tgcaagcggg cgaccagatc cgggttcgca atccagtcac gaccaaatgc caccgcatca
    19381 attaaccctt tgccgatcag cgtttcagct ttttctactg tgtatgcacc tgcgccgata
    19441 atcggaccgt ggaaacgggc gcgtactttt tcgcggaacg catcagtata cggttcaccc
    19501 cccgcccaat ctggttctga catatgcaga taagcaatgc cgcgtttacc cagttgttca
    19561 atcagataca gtgcatcggc ttcttcattc gggccgttat ccgtgttctg gaaagtaccg
    19621 attggcgaaa tgcgaatgcc aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc
    19681 acttccagta ccagacgtgc gcgattttcc acgctgccgc cgtactgatc ggtacgatgg
    19741 tttgaagaag gagaaaggaa ctgatgcagc aaataaccgt gagcagagag gagctctacc
    19801 agatcaaaac cggcttcacg cgcgttagca atggcctgac ggaaatcatt gacgatacct
    19861 ggaatctctt ctagttcaag cgcacgcggc atggatgttt caacacggat cgcctgacca
    19921 ttttcatcgc gcagagaagt acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc
    19981 tgaccgccag gttgcaggct ggcgtgagaa atgcgtccgg tgtgccacag ctgcacggcc
    20041 atatgaccat tttcagcatg aacgccagcg gtgatttttt tccatgcggc aatttgctcc
    20101 ggactatgga tgccaggcgc acctgcatat ccttttgcct gggcagaaat ttgcgtggct
    20161 tcactaataa tcaaaccggc actggcacgt tggcgatagt attccgccat caacggggta
    20221 ggaatgtcac ccggttcaat actgcgcaga cgcgtcagcg gtgccataaa aatacggttt
    20281 gccgccgtga tcgcgcccac tttcagtggg gaatacagtt tttcagatga cataaaggct
    20341 cccggtcgtc tggtgataaa gaatgcctgc taaacggcag gcgtcgcaat aatgtttttt
    20401 acatgggcca gcgcgttttc cagtggctcg aaactgcgcg aaattttggc ctgcagattc
    20461 gcacccagcc atagtgcgta aagcacttgt gcctgttgta gcggttcgcc acaaaaggtt
    20521 aaacaatggt tctcacggcc attttccagc gcctgagaga gcagggcgat cacgccgcga
    20581 gcacctttat ccatcgcgct gcgcatatct tctgacagat cgcacacttc ggcagagagt
    20641 tttactgtca ggcaaccact gatggttcca tgttggcaaa actggttcag tgtttgctgg
    20701 taataagcca gtatgcggtc gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt
    20761 cgctggtgat atgcggcgta atgacgctca agcatggcaa cgccaaacgc ttctttagag
    20821 cgaaagtagt gatagaagga ccctttcggc acttcagcgg tttttagtaa ttcgcttagc
    20881 cccatcccgg tgaatccacg ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg
    20941 cgagtatcat gttcggtgtg tttgttcatg cgttggagtg tagtagacca gtcggtctag
    21001 tgcaagcaaa gttgctactt tggacattaa cagcttcaat aatggtaata atcttatctg
    21061 aggagggcta agtggcggag caattagagt tctttcccgt ccagagcccg tgccggggaa
    21121 tttgccagtc tgacgaacgc gggttttgtc gcggctgttt tcgtagtcgg gatgagcgtt
    21181 ttaactggaa taaaatgagc gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc
    21241 gattaatgcg caaattacgc gcaaataagc cagcgtcatc agacgaaccg gagcaaccat
    21301 cactcttttg aacctaatct tgcgtatact cctctcatat ctgataagag gaagcgatta
    21361 tggttcagcg tattactatt gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct
    21421 actggcgatt gatggactgg aatatgtccg cccgccagct ggtcagtttt attgaagagc
    21481 atctggatct cggcgtgacc accgtggacc atgctgatat ttatggtggc tatcagtgcg
    21541 aagcggcgtt tggcgaggca ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg
    21601 tcagtaaatg cggtatcgcg acgaccgcgc gtgaagaaaa cgtcattggt cattacatca
    21661 ctgaccgcga tcacatcatt aagagcgccg aacagtcgct aattaatctc gcgaccgatc
    21721 atctggatct gctgttaatc caccgaccag acccgttaat ggatgccgat gaagtggcgg
    21781 acgcgttcaa acatctgcat cagagcggca aagtgcgtca ttttggcgta tcgaacttta
    21841 cgcctgcgca atttgccctg ttgcaatcac gtctgccgtt tacccttgcc actaatcagg
    21901 tggaaatatc cccggtgcat cagccgttac tgctggatgg cacgctcgac caactacaac
    21961 aactgcgtgt tcgtccgatg gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg
    22021 attatttcca gccgctgcgt gatgaactgg ctgtggtggc agaggagtta aacgcgggct
    22081 cgattgaaca ggtggtttac gcctgggtat tacgtttacc atcgcagccg ctgccaatta
    22141 tcggttcagg taaaattgag cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga
    22201 cccgtcaaca atggtttcgt atccgtaaag cggcactggg gtacgacgta ccgtaagcac
    22261 ttttaggaat agccgccgtt caaaaatgtg tcactggttt acacttattc aggaatgcac
    22321 aatgaacgga ggtcctatga aacgttttag tctggctatt ctggcgctgg ttgttgcaac
    22381 cggcgcacaa gctgccagtg aaaaagtcga gatgaacctc gtcacgtcgc aaggggtagg
    22441 gcagtcaatt ggtagcgtca ccattactga aaccgataaa ggtctggagt tttcgcccga
    22501 tctgaaagca ttaccccccg gtgaacatgg cttccatatt catgccaaag gaagctgcca
    22561 gccagccacc aaagatggca aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc
    22621 acaaaatacc ggtaaacatg aagggccaga aggtgccggg catttaggcg atctgcctgc
    22681 actggtcgtc aataatgacg gcaaagctac cgatgccgtc atcacgcctc gtctgaaatc
    22741 actggatgaa atcaaagaca aagcgctgat ggtccacgtt ggcggcgata atatgtccga
    22801 tcaacctaaa ccgctgggcg gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt
    22861 aagaggcctg cgtaccttgc ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc
    22921 gccagacaat tgccgccagc tcacgggcgg caggttgatg atgacgggca aggctgtcgc
    22981 aaatccgctg taattcttca agtgtggccg ccagtgattt ttgctgaacg ccacgctcac
    23041 tcatcactcc ccgcaacagt gaaatacagt tatcccgtac tcgcgataac ggatcggaac
    23101 gcgattccca gtcgcgcaat tgccagacaa catgagaaca gttcagcagc actacacccc
    23161 agcgtaataa ccaacggcga gccagcgcat cctgctgtta ctcaactgac tgacgtgatg
    23221 ataagtgagc gattcaaatt cgctttcact cagtgttgga tggcggctta gctgatcgac
    23281 aaaatcccgg cgcagcgcgc gaatatggcg gcggctttta cgagcatccg atcctggacg
    23341 cagaatggcg aacgctagcc aagccagcgc aacgccaacg atttttgcca gattatcgtt
    23401 aagaaaatca gcaaaatcat acaccggggg attagtgaca gcgataaacg aacccataaa
    23461 aacaatcagt tgcccccaca gtgcggcaaa tttgggcatc tgcaatttaa gaagctgcat
    23521 tgtcgccagc agtggaaaga gaaacagtaa aaattgccac agatcgctaa tctggaccat
    23581 caggccaaat ttgaccacaa agctgaatag cgaaagtaac accagcgtgc gcatcagcag
    23641 tgacaacgac ttaaacggtg ctgcgacggc ggagtagagt acgcagctaa ttgctgccag
    23701 cgttaatgca ttggcaccgg catcccattg cgatgcaata ctccatgcgc caatcatcat
    23761 caacgtacaa aatgtacgca gcccgctcca catagcttcg gcgttgtcgg tatgacgagc
    23821 cagagcggat gtccgtgcga gtctggcatg atcatctaca ccgctttgca gacgatgtaa
    23881 ttcctgacta ctttgcagat aaaggcggca aaaataacgt agtcgctgcc agaaggcgac
    23941 gtgccggtag tcggcgacgt tggtcgggcg tagcggggcg ataatacgtg cgacggtgta
    24001 agcatctgtt tgcgaactgg cgagcgccgt cagcaattgt tcgagaattt ctcgcgtggc
    24061 acctggcggt gagggccagt tgagcaacat acgtcgcagg ctggagatga tactggtcat
    24121 acgtaattgc tggtggagta gcgcattaag gcgcgcgttt tgctggcgaa aacgatagtg
    24181 gctccagaaa gcctggatac gcagcaaatt catggtcagt atctgcccaa tccccccctc
    24241 atgtgctgca cgaatggcat cgtttgtttc aggctgccag agtaaactgg catgttccag
    24301 taatcgggcg tgcatgtttt tcaatgcggt taaaagggct gtagcatcgg aactgctagg
    24361 caggatcatc atcatcatgc agccgcacaa aataccgact atcacctcgc aaacgcgcgc
    24421 ctgagcgata tcccacagct ggctggcctc agtaatatta accatcggaa aggcgatgat
    24481 ggcagccgtg tagcccgcca gttgaaatgc atacgcgacg ttattcgtga agtgcgcaca
    24541 ggcccaggta caaaagccaa gccacgccga catgctcaat agaaaaaacc acggctcatt
    24601 gagcgtatgc ccggcaagaa gcagtgccgc aatggctccc agtaaactgc cagcgatgcg
    24661 tccgaggctt ttgctgatga caccgccaac ggtgggaaag ctaaccactg cagccgaggt
    24721 catcgcccaa tagggttcat ccagattcaa ataataggca accgtcagcg ccagacacat
    24781 ggcaatggta ttgcgtaacg cataacgcca ttgcgccagc gtggccttga accagggcaa
    24841 attgcgcaag gaccatgacg atgcgttcat tattgaccta cggcaatgct acaggtggtg
    24901 cccgacacca gcgtgacatc gcctggaact ttatccagcg caaagcggac gggaacgcgt
    24961 tgggcgagac gaacccaggg gacgttaggt ttcacatccg gaataaggct tgagtcactt
    25021 tcaacgcttt gatcataaat cgcccgaccg atactggaaa cgtgaccccg taacgttttg
    25081 ttatcactat agagggtaat ttgtgctggt gcaccttcgc ggatatggcg caacttggtt
    25141 tcttcaaaat aaccaatgac gtaaaacgag tggctgtcga caagggcaaa cagcggtttc
    25201 ccggtgtcag cgtaatcgcc gatgcgggta gtgaggttag tcacccaacc actcaccggg
    25261 gcgcgaattt ccgtttgcgc cagttgccat tgtgtctgtt tcagcgtggc ctgtgcggca
    25321 ttaacactgg cctgcatcgc tttaacattg aggttagcgg tatcgagttc ttcggcagaa
    25381 ataaaatttt gtgagagatg acgacgacga ttcgcttcgt tattggcttt tgcgaggtca
    25441 gattgcgctt tcgccagctg agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta
    25501 tcgatcgtga gcagaagatc ccccgcgtta accagttggt tgtcttttat attcagctca
    25561 acaatacgtc ccgacacctg tggggtgata gaaacctttt ctgcgcgtat tttcccgtcg
    25621 cgtgtccacg gagattgcat gtaataattc cacagccacc atccggcaag aaccgcaact
    25681 atcgctacaa tgattgttga gaaatactta attgttttaa ttgacatagt taccacgcaa
    25741 tcagtattgc cagagcaagg caaacgcaaa tcgcaaacag cgataaatcc atttacaagg
    25801 gatgccagat gtcaccggcg tagatccagc cacgaagcag gcggtgtacg acaagccaga
    25861 tgacgaatcc aaacgcgaag gctttgaaaa acggaggaaa atagacggac gcaccgaaca
    25921 ccagatcttg taagggcaat cctgttgcat tgagcataaa cttcacaaag tagattcctt
    25981 tacgaccgaa tatattgcgt tagattaata aatattcttt aagtgcgaaa aatttacgcg
    26041 caatttctga aaaatatgtc tgacggtaac caaatgcagc aatacatttg ttttagcaat
    26101 aaaattgctg cacactactc taaaagccgc ataatatctt agcaagctaa ttataaggag
    26161 atgaaattgg aatcgccact aggttctgat ctggcacggt tggtgcgcat atggcgtgct
    26221 ctgatagacc atcgcctgaa accgctggag ttaacacaaa cccattgggt tacgttacac
    26281 aatatccatc agttacctcc agaccagtcg caaattcaac tggcaaaagc gattggcatc
    26341 gagcagccat cactggtccg tactctggac caactggaag aaaaagggtt aatttcgcgt
    26401 caaacttgtg ccagcgatcg tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca
    26461 ctgatcagcg aaatggaagc tgttattaac aaaacccgcg cggaaatatt acatggcatc
    26521 tccgcagagg aactggagca actgattacg ctcatcgcaa aacttgagca taatatcatt
    26581 gagttacagg ctaaagggtg aaatgaaggg ggcgtgtggt cagtaacctg accacatgca
    26641 aacttattta gcgcggagaa acggtcacct gactgccatt gctggccagt acgacacgtt
    26701 ggcccggaga gaaacgagtg ttgccttgtt tctgtaccac catgatggta ttaccatcgt
    26761 ctttacgaat ttccagctcg acaccctgcg ttttgttcat tgcactctgt acgccctgac
    26821 cagctacgcc acctgcaaca gcgcctgctg cagtagccag agaacgcccg gttccgccac
    26881 caacagtatt ccccaggaaa ccaccaagaa cagcaccgcc aattgcaccg ataacgttgg
    26941 aatcatcacc gccctgaatc tgtaccggac gtacgttaac gatggtgcca tagctgacat
    27001 tctgtacttg tttcgcttca gaagcggtat aaacatcccc tgacagggtg tcgttattaa
    27061 cacaaccgac aagagacaga cctaccattg aaacaaccaa tacgcgttta atcattgaaa
    27121 aatctcctgt tcaccatgaa acgccacgca agcatccctc atggtcaaag tatatggcat
    27181 atgtgaatcc atggttacat gttctaccaa atatgagcaa atcataatga aagtaggatc
    27241 aaccaattat aaacaaaaca ttcatgatga ttattgattt gaagataatg ttttactacc
    27301 aacggtaaaa atcggtcatt ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg
    27361 caaacgtcag attgaaaaag gatgaactat gaaatcgggc cgctttattg gcgttatgtc
    27421 aggcaccagc cttgatggtg ttgatgttgt gttggcgaca atagatgaac accgggtcgc
    27481 acagctggca agcttgagct ggccgatccc ggtgtctctg aaacaggctg tactggatat
    27541 ttgccagggc cagcagctta cactttcgca gtttggacag cttgatactc aactagggcg
    27601 actttttgct gatgcggtca atgccttgct taaggaacaa aacctgcagg cgagagatat
    27661 agttgcgatc ggctgtcacg gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca
    27721 cactttacag attggcgata acaatcaaat tgtggcacgc accggaatta cggttgtcgg
    27781 tgattttcgc cgtcgcgata ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt
    27841 ccatcatgcc ttgctggctc acccaaccga gcgacgaatg gtgctcaata ttggcggcat
    27901 cgccaatctg tcactgctca ttcctgggca gccggttggc ggctacgata ccggtcctgg
    27961 taacatgctg atggatgcct ggatctggcg tcaggccggt aaaccttacg ataaagatgc
    28021 cgagtgggca cgggcgggta aagttattct cccactgctg caaaatatgc tcagcgaccc
    28081 atatttctcg caacctgcac cgaaaagcac cggacgcgaa tactttaact acggctggct
    28141 ggagcgccat ttacgccatt ttccgggtgt tgatccccgt gatgttcagg ccacactggc
    28201 agaactcacc gccgtgacca tttctgaaca agttttgttg agcggtggct gcgaacgatt
    28261 gatggtatgt ggtggaggta gtcgtaatcc gctactcatg gcgcgtctgg cggcattact
    28321 gccaggcaca gaagtcacca ccaccgatgc cgttggcatt agtggcgatg acatggaagc
    28381 attggctttc gcctggcttg cctggcggac gctggcggga ttaccaggaa atctgccttc
    28441 cgtcactggc gcaagccagg agacggtact gggggctatt ttccctgcta acccgtgata
    28501 tcagagttaa ctgaattttt cttaccgcgt ctgccgttac acttggaacg ttagggaggg
    28561 cgtattgccc tccagaccag gaaagtcttc gggaaatgtc tatgaaaaaa ctgttactga
    28621 tctgtttgcc ggtgcttctc accggctgta gcgcgttcaa tcaactggtt gagcacatgc
    28681 aaaccgctac gctggaatac cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc
    28741 cgcgccagga agtcagtttt gtttacgata atcaactact gcatctcaaa cagggcattt
    28801 cagcgtctgg tgcacgttac actgacggaa tctatgtttt ctggtcgaaa ggcgatgaag
    28861 cgactgtcta taaacgcgac cgcatcgtat tgaataactg tcagttacaa aatccacagc
    28921 gttgagattt ttccaggggc ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa
    28981 accatgtctg ataacgacga attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa
    29041 ggcgggttac gccgtcgcga tcttcccgcc gatccattaa gcctttttga acgttggctc
    29101 tctcaggcgt gtgaagccaa actggcagac cctaccgcga tggtggtcgc taccgtggat
    29161 gaacatgctc agccttatca acgcatcgtt ttactcaaac attacgacga aaaaggcatg
    29221 gtgttttaca ccaacctcgg tagccgtaaa gcacatcaaa ttgaaaataa tccgcgcgtt
    29281 agcctgctgt tcccgtggca tacccttgaa cgccaggtga tggtgatcgg taaagcagaa
    29341 cgactttcga ctctcgaagt gatgaaatat tttcatagcc gcccgcgtga tagccagatt
    29401 ggtgcatggg tttcgaagca gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa
    29461 ttcctggagc tgaagcagaa gtttcaacag ggcgaagtgc cattgccgag cttttggggc
    29521 ggttttcgcg tcagccttga acagattgag ttctggcagg gcggtgagca tcgcctgcat
    29581 gaccgctttt tgtaccagcg agaaaatgat gtgtggaaga ttgatcgtct tgcaccctga
    29641 aaagatgcaa aaatcttgct ttaatcgctg gtactcctga ttctggcact ttattctatg
    29701 tctctttcgc atctggcgaa aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg
    29761 agattttgat ggcaagcagt aacttgatta aacaattgca agagcggggg ctggtagccc
    29821 agatgacgga cgaggaagcg ttagcagagc gactggcgca aggcccgatc gcgctctatt
    29881 gcggcttcga tcctaccgct gacagcttgc atttggggca tcttgttcca ttgttatgcc
    29941 tgaaacgctt ccagcaggcg ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc
    30001 tgattggcga cccgagcttc aaagctgccg agcgtaagct gaacaccgaa gaaactgttc
    30061 aggagtgggt ggacaaaatc cgtaagcagg ttgccccgtt cctcgatttc gactgtggag
    30121 aaaactctgc tatcgcggcg aacaactatg actggttcgg caatatgaat gtgctgacct
    30181 tcctgcgcga tattggcaaa cacttctccg ttaaccagat gatcaacaaa gaagcggtta
    30241 agcagcgtct caaccgtgaa gatcagggga tttcgttcac tgagttttcc tacaacctgt
    30301 tgcagggtta tgacttcgcc tgtctgaaca aacagtacgg tgtggtgctg caaattggtg
    30361 gttctgacca gtggggtaac atcacttctg gtatcgacct gacccgtcgt ctgcatcaga
    30421 atcaggtgtt tggcctgacc gttccgctga tcactaaagc agatggcacc aaatttggta
    30481 aaactgaagg cggcgcagtc tggttggatc cgaagaaaac cagcccgtac aaattctacc
    30541 agttctggat caacactgcg gatgccgacg tttaccgctt cctgaagttc ttcaccttta
    30601 tgagcattga agagatcaac gccctggaag aagaagataa aaacagcggt aaagcaccgc
    30661 gcgcccagta tgtactggcg gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac
    30721 aggcggcaaa acgtattacc gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag
    30781 cggacttcga acagctggcg caggacggcg taccgatggt tgagatggaa aagggcgcag
    30841 acctgatgca ggcgctggtc gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa
    30901 ctatcgcctc caatgccatc accattaacg gtgaaaaaca gtccgatcct gaatacttct
    30961 ttaaagaaga agatcgtctg tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt
    31021 actgtctgat ttgctggaaa taatgcatta agtggaaagg gggagtgaga aatcactccc
    31081 cctggttttt atacagggaa catgatgaaa aatattctcg ctatccagtc tcacgttgtt
    31141 tatggtcatg cgggtaacag tgcggcagag tttcctatgc gccgcctagg cgcgaacgtc
    31201 tggccgctga acaccgttca attttctaat cacacccagt acggcaaatg gactggctgc
    31261 gtgatgccgc ccagccattt aaccgaaatt gtgcaaggca ttgccgccat tgataaatta
    31321 cacacctgtg atgccgtgtt aagtggctat ctgggatcgg cggagcaggg tgaacatatc
    31381 ctcggtatcg tccgtcaggt gaaagccgcg aatccgcagg cgaaatattt ttgcgatccg
    31441 gtaatgggtc atccggaaaa aggctgtatc gttgcaccgg gtgtcgcaga gtttcatgtg
    31501 cggcacggtt tgcctgccag cgatatcatt gcgccgaatc tggttgagct ggaaatactc
    31561 tgtgagcatg cggtaaataa cgtcgaagaa gcggttctgg cagcgcgcga actcattgcg
    31621 caagggccac aaattgtgtt ggttaaacac ctggcgcgag ctggctacag ccgtgaccgt
    31681 tttgaaatgc tgctggtcac cgccgatgaa gcctggcata tcagccgtcc tctggtggat
    31741 tttggtatgc gccagccggt aggtgttggt gatgtgacga gcggtttact gctagtgaaa
    31801 ctgcttcagg gggcaacgct gcaggaggcg ctggaacatg tgaccgctgc agtctacgaa
    31861 atcatggtga ccaccaaagc aatgcaggaa tatgagctgc aagtggtggc tgctcaggat
    31921 cgtattgcca aaccagaaca ttacttcagc gcaacaaagc tctgaaatat ctcatttcgg
    31981 cccacaacga cctgtgggcc gatggctatt actttaagcc ttccgctgac agcgcgtctt
    32041 gtacttccgg acgttcagcc atacgttgca taaatgctgc aatgtgctct aacccttcca
    32101 gattcagttt caccgcgtat gcccagcgca gaaccgtaaa cagataggca tcagcaattg
    32161 taaatctttg cccgcagatc cagtgctcat ccttcagtgc ctcgttcaca tattgcagct
    32221 tcttctccag ctgcgcgcga actgtcggtt tgtactcttc cggtgtatca gggcgaaaca
    32281 gaggtgtgaa acctttatgc agctcggtgg cgatgtaatt cagccattcg atggttttat
    32341 agcgggaaat actgtttacc ggtgccagca actggcggtc ggggacgctg tcggcaagat
    32401 actgcataat cgctacgcct tccgtcagca aagtatcgtc atccagcagc aatgcaggca
    32461 cctgcccctt agggttaacg gcaaagtaat cgtcaccgtt ttcgagacgt tttttcatta
    32521 aatccacact gacgagggta aaatcctttc cgctctcacg cagggtgata tgggaagcga
    32581 gagagcaggc acccggtttg tagaacaatt tcatcggtaa ctccttttgt ctgtggttta
    32641 aagtatggta gtgcgttgct gaacaaaaaa aaagccgcta acgaaaagtt agcggcttgt
    32701 aaaagagttt ccctgaattt tacgctacag cagctttcgc cgctttgtct gcagcgtcat
    32761 cctgcgtcat acggtgcagt ttaggcgcgg tcagcagcat cagtactgca ataatggcag
    32821 tagcgacacc aatctgcaag aatacgcgac cataaacttc cagtgacatc agcgggtcgg
    32881 taacgttatc cggcacagcc atcatacccg caacataacc accaatcagg tttgcaccgg
    32941 cagtggtcag gaaccagcta cccataatga agcccatcag acgctgcgga acgagttgtg
    33001 caaccattgc cagacccaga ccagagatca tcagttcccc gatgctctgc aggccatagc
    33061 ttgcgaccag ccagcttaca gacacgatac cagcgtcaga cgcgaatttc gcacccagcg
    33121 gcagaatcag gaacgcacca gaacacatca ccatgccgat tgcaaacttg gttggcatcg
    33181 gcagggtatc gcccatcttg ttatagatag cggccagaat cggactaccg atgatgatcc
    33241 agaacgggtt cagtgcctga tactgctcag gttctacggc cagacccaga atggagtgct
    33301 caacgttacg aatcgcaaag aagttcagtg acgttggcat ctggctgtac agcacgaaga
    33361 agataatggc ttcgagcatc aggatgaagg caacgatcat tttacgacgc gcagcacctt
    33421 tcatcgcgaa ggcttcttta ccgaagataa ccacgatacc gaaggcaaca acgcccagcg
    33481 ccatacgcgc aacttcctga ttgtgcagca gccaggtggc gatagcgatc agtgccacaa
    33541 caccaataat ggtcagcagc aggttacggt agttgatagg ctcgaagtct ggttttgaac
    33601 cgtactgttt aacccagcgt tggcagaagg cgaagttaac gatagtgatc agcaggccta
    33661 caacgctcaa cgcaaacgca acactccagc cgtatttcgc ggccagccac ggcgtagcaa
    33721 tcatagagaa gaaagagccg atgttgacgg acatgtagta catggtgaat gcaccgtcca
    33781 gacgcgggtc gtttttctca tagcatgtag aaagcagaga agacgggtta gctttaaaca
    33841 ggccgttacc gaccgcaata gccgccatac ccatataaac gataccggcg tcgtgaccag
    33901 accaggcaac cagagcataa ccaatcgcca gcacaatagc gccgagcata attacgcgtt
    33961 tagtacccag taccttgtca cctaaccagc cgccgatagc gaccagacca taaaccaggg
    34021 cactaaagga agagaaaagg gtgattgaat ccgcttcaga catacccagt tgtttaacca
    34081 ggtaaacagc cataattcct tgtaggccgt aataaccaaa acgttcccat aactcaatcg
    34141 agaagatgag atagaacgcc ttcggttgtt tgaaagcgtt caaactgacg ctttcagttg
    34201 gtttttggtt tgcagtggac acttttacct ctttttttac atcccatatt aacgggggtg
    34261 ctttctcgcg ttatgtctaa aaaacatacg cgcaattgtt atagtgggag gggaaacggc
    34321 aggtaatgtt cactatcttg cactttcaga caagtccttt gtaataatct gttacatata
    34381 actgaggcgg ggaattcaac cattccggga aatgttatcc agcgttaaat ttttcaaaaa
    34441 acgtaacggc agattttttc actgcactga gaggggggtg atggcttaac ggcgatatgt
    34501 tctgctattt agcgttttta ctagtgagat agtccagttt ctgaaaaata gccagtataa
    34561 tgttttgtag gtcaatattt gttgcgtgtg taaatttaaa tgattttaac ctgattcttg
    34621 caatgttttt acattgcgac agcatcgtga tcttgatcac gaatgaatag aaaatttcgc
    34681 tattaaaaaa acaattaacc tgcattttta gttatccgca aacggattac ctgatgataa
    34741 cgctaagcaa tggcatcatt cacgttaaaa agatgtttta aaagaggata aagaaaggtt
    34801 atcaatgggg taatcggtgt tacccctttt cttcagatgt caactttctc tttgtattca
    34861 caaagatctt caataataca agagccacag cggggcttgc gggcaatgca ggtataacgc
    34921 ccgtgcagga tcaaccaatg gtggcagtcg actttaaact ctgccggaac cactttcagt
    34981 agcttttctt ctacctgttc aacgtttttc cccggcgcaa attgagtacg attacaaacg
    35041 cggaaaatgt gcgtgtcgac agcaatagtc ggccagccga atgcagtgtt taatacgacg
    35101 ttggctgttt tacgacctac gccgggcagg gcttcaagcg cagcacgatc ttccggaacc
    35161 tcgccattat gctgctccag taagatacgg caggttttga tgatattttc tgctttgctg
    35221 ttataaagcc caatcgtttt gatataggtt ttaacccctt caacgcccag ttcaagcatc
    35281 gctgcaggcg tattcgccac cgggtagagt ttcgccgtcg ccttattaac actgacatcg
    35341 gtcgcctgag cggaaagcag tacggcaatc agcaattcaa aaggcgaact gaaattaagc
    35401 tcggtggtgg gatgaggatt gttctcacgc aggcgagtga ggatctccag gcgttttgct
    35461 ttgttcatca gacattccct gtttcaccgt ttggcaatgc acgttcggca gctgcttcag
    35521 tacggcgttt tttcattctt tcatcaatca ggtattttcc tgccagcatc agtcccaggc
    35581 caataaatgc acctggtggc agcatcgcca gcaggaaagg ggagtcggtg tggaaaatct
    35641 ccacgcgtaa tacttttgcc cagctaccta acagcgcatc tgcaccgtca aacaatgtgc
    35701 cattgccgat aatttcgcgt agtgaaccca gcacgaacat ggcacaggtt gcgcccatac
    35761 caattgaaaa gccgtccagt gccgaaagcg ccggaccttt tttggcggcg aaggcttcag
    35821 cgcggcccac aacgatacag ttagtgacaa tcagcggaat aaaaatccct aatgattgat
    35881 acaggccaaa ggcgtaggcg ttgatcagca tctgtacagc gctgaccacc gaggcgatga
    35941 tcatcacgta aatgggaatg cggatctcgg ctggcgtcca gtgacgcagc gtcgaaatgg
    36001 tcaggttggt cagcgtcagt accagcgtag tcgcaagtcc taaacccaga gcattagtgg
    36061 cagtggacgt gaccgccaac agaggacaaa ggccgagcaa ctggaccagc gcagagttgt
    36121 ttttccacaa cccctgaaca ataacgtctt taatttcgct cacgctttat tctccacagg
    36181 caggaagttg agaaagttgt gccggtaacg tctgagcgta caatccggcg cgttttaccg
    36241 cattaaccac cgcgcgggga gtaatcgtcg cgccggtgaa ctggtcgaaa tcaccaccat
    36301 ctttcttcac cgcccagtgc gcatcatccg caccactgat ttttttaccc gcaaaatggg
    36361 tgatccagtc agaaaggcgc agttcgattt tatcgccaag ccctggcgtt tcgtggtgct
    36421 ctgtcacgcg cgtgccaagt accgtgccgt taaaatcggc tcccaccagc agctgaatcg
    36481 caccggaata gccatctggc gcggttgctt ccagaacggc ggctaccggt ttgtcatcct
    36541 gtttggcgat gtaaacccga tgctcacctt tacctaactc tggcgcagtt accagatagc
    36601 aactctgtgc cagcgcattg ttatagcgtt cggctggcag cacctgatca aataatgcct
    36661 tttgttgcag acttgcctgt tcagcaatcg tcgttttggt catctggttg atggccgcag
    36721 ttaaccctgt tgaacccgct gcaaatagcg ctaacgtaat gccgtgtttt cggatagttt
    36781 tcagcatggt ttaccctttg cgatggccgt agacgcgcgg acgcgtgtag taatcgatca
    36841 gaggaaccgt gatgttcgcc agcaggacgg caaaagccac gccgtcagga tagccgccga
    36901 aactgcggat caaccagact aataagcccg caagcgcgcc gaaaataaga cgaccacgat
    36961 tggtcgtaga agcggtaacc gggtcagtca aaataaagaa tgcgccgagc atggtcgcac
    37021 cagacagcag atgaatttgc ggtgctgcca gtgtttctgg tgagaacaac cagcccaacg
    37081 ttgcgcataa cgccagcgtt actaagaagc tgaggggaat atgccagcga atcgctttct
    37141 gccatagcaa ccacaggccg ccagccagcc aggcgagatt tacccattgc caaccagcgc
    37201 ccgccagaat accgctgtag atcggatatt gcataatctg ttcaaccgaa tgaccggcac
    37261 ggacagaggt tttaaatgta tccagcggtg tcgcctgact aatgccatca atacctaagt
    37321 gtagtgtgtt catatcagca ccactggcgg tatgcccgct gaaaataacc tgtatggcgt
    37381 cgataaaacc agggatgttg accgcaattt catgtggcgg taaccagctg gtcatctgca
    37441 ccgggaagga gatcagtaag accacataac caatcattgc cggattaaac gggttttgcc
    37501 ccagaccgcc atacaactgt ttagcgatga tcaccgcaaa caccgaaccc agcacgacca
    37561 tccaccatgg cgcgaggggg ggaatactta ccgccagcaa taagcctgtc agcaatgctg
    37621 agttatcttt caacgttgcg gctaccgact gcttgcgtag tttgagtacg agagcttcgg
    37681 ctaacagagt actaaccgat gccaacagga tctgaacgag agtaccccaa ccaaaaaacc
    37741 acagttgcgc tgcgattcct ggcacggctg cgagcaacac caacagcata atgcgcgatg
    37801 tctggcgctg gttatgggta taaggggagc tagctattct gaataccatt tagtcctcgt
    37861 ttacaacctt ttgctgggcg gcttttttgg cctgagcgcg ggcaatagcc gcggcaactg
    37921 ccgctttgcg cggatcaacc tgttcttctg gttctgcgtt agtctgttgc tgttccagct
    37981 tgcgcgcttt ggcacgggca atagcggctt cgacggcggc tttgcgcgga tcaacctgtt
    38041 cttcaggtac cgcattagcc tgttgctgtt ccagtttgcg cgctttggct cgggcaatag
    38101 cggcttcgat ggcggctttg cgcggatcaa cctgttcttc tggttccgca ttagcctgtt
    38161 gctgttccag cttgcgcgct ttggcacggg caatagcggc ttcgacggcg gctttgcgcg
    38221 gatcgacctg ttcttctggt tccgcattag cctgttgctg ttccagcttg cgcgctttgg
    38281 cgcgggcgat agctgcttca acggcagttt tacgtggatc agcaacggtt gctgcgtcgt
    38341 tagtttgctg cagttctgcc tgtttcgctc tggcttgcgc tttacgggct tcccgtgctg
    38401 caataattgc actgttatcc gggcgttcgc ccgctttaat cacaataggc tgtgtagcct
    38461 gggcctgttt ctctttcacc cgcgccagag cggcagcaat cgcatcttta tctttggctg
    38521 caggttgaac ggctgcgctc ttatgtcgtt caaggcgagc cgctttttcg cgctccagac
    38581 gagcctggcg cgcttcgaaa cgcgctttgg cttctgcggc gcgcttttct tcctgacgaa
    38641 tagccgcaat ttcagctttt tcctgacgga aatattgcac caggggaata ttgctcgggc
    38701 aaacccacgc gcaagcccca cattcaatgc aatcagcaat gttatgcgtg gtagctttat
    38761 cgtgttgctg acctttgctg aaccagtaca actgttgcgg caaaagatcc gcagggcagg
    38821 cgtcagcaca ggcgctacac cggatgcagc tttgttcttc ctgtggttcg ccaagttcat
    38881 tggcagaggg agccaacaga cagttggtaa tctttacgac cgggacatcc agccatggca
    38941 aggtaaagcc cattagcggg ccacccataa tcaccatttg atcggcagag gggcagaatc
    39001 gggcatcatt caataaatga cgcactggcg tccccagccg tgcccagacg ttgcccgggc
    39061 gagcgattgc ttcgccagtc agggttacaa cacgctcggt aatcggctcg ccatcaataa
    39121 cggcacgttt cactgcataa gcagtgccga cgttttgcat taatacgccg atatcggatg
    39181 aacgcccgcc atgtggaacc tgcttcccgg tcagaatgta ggttaattgt ttagcaccgc
    39241 cagaaggata tttggttgga atcacccgca gagaaatatc gttagagtcc gccagcaccg
    39301 cgcgcagcat ggaaatcgcc tgcggtttgt tatcttcaat gccgataaga atttcgcgtg
    39361 gctgcagaat atgcgcaaga atgcgaatac cctctacgac ctgagccgcg caatcctgca
    39421 tcaaacggtc atcggcggta atgtacggct cgcactcagc cgcgttgata atcaacgttt
    39481 caatcttatc tccgccaccc tgcaatttaa cgcctgtcgg gaatcctgca ccgcccagcc
    39541 cggcaacacc aaactgatgt atgcgctcga ttaactcttc gcgactgcga gtgcgataat
    39601 cggcccagcc gtcgcgcggg atccagcagt cttcaccatc ggcatcaata atcacgctta
    39661 attcagctaa agctgaagga tgagccgtag agtggggcgc aatagccgta acggtacccg
    39721 aggtgggcgc gtgaacaggc agcattttgc cgcgaccacg ggtaagcggc tggccgcgca
    39781 atactttatc gccgacgcta acgcacaact caccttcagc gccaatatgc tgtttcagtg
    39841 gaataacaaa acgctgcgcc agtggtacct ggcgcagggg tgtaccgttg gactgggttt
    39901 tcatctccgg tggatggatg ccgccgttga aatcccagat tttatttttt ctgaatgcag
    39961 agaataactt aagcatggtg ttccacggga atgatacgca cgggaatggt gttcagatcc
    40021 catttccagg agtcaggtgt ttctgcgacc ggttgcaacg agatgcagtg cgtcgggcac
    40081 ggatcaacac ataaattgca gcccgtacag agatcactca ttaccgtatg catggcacgg
    40141 gtagcgccaa cgatggcgtc taccggacaa gcctgaatac atttagtgca gccaatacag
    40201 ttattttcat caataaccgc caccatccgc gcaggcgtta gctcttgcgc ttcgccatcc
    40261 agcggctgcg gctcgacatt aagcaactcg gcaattttta gcatcacagc ttcgccacct
    40321 ggggcgcaac ggttgatttt ttcaccgtta cagctgatgg cttccgcgta ggggcgacag
    40381 ccgggataac cgcactgacc acactggctc tgcggtaaga tttcgtcaat tttctcaacg
    40441 accggatcgt cttccaccgc aaaacggcgg gaggcataac ccagaatggc gccaaacgcc
    40501 aggcccagca ggctcacggc ggcaacggca atccagatag cattcattac aacttcacca
    40561 aaccactaaa gcccataaag gccagagaca taagacctgc ggtaattaac gcaatggcat
    40621 taccgcgaaa aggtgccggg acatcagcca cagcaaggcg ttcgcggatg gcggcgaaga
    40681 gcaccatcac cagcgagaaa ccgacagcgg cggaaaaacc gtacagcgcc gactgcaaga
    40741 aattgtgccc gagattgata ttcagcaacg ccacgccgag cactgcacag ttggtggtga
    40801 taagcggcaa aaaaatcccc agcagacggt aaagcaccgg gctggttttg cgcaccacca
    40861 tctcggtgaa ctgcacgacc acagcaatca ccagaataaa tgccagggtg cgcaggtaaa
    40921 tcagattaag tgggatcaaa atccacttat cgataagcca ggcgcaaata gacgccagcg
    40981 tcatcacaaa cgttgttgcc agccccatgc ccatcgcggt ttccagtttt ttggaaaccc
    41041 ccataaacgg acagagaccg agaaacttga ccagtacaaa gttattgacc agtacagttc
    41101 cgacaaacag taacaggtag tcagtcattt ttcggcctga aataaaaaag ccgcctatta
    41161 tccgttaatc cagagcaggc gacaacaggt taactgtaag gttattacgg attcacaaac
    41221 gtcgctttta cccgcgacga gcgtttgaaa taggagacga tcaatgcggt tgccaacaaa
    41281 gtaaacagca actggcgaac ggctatgcca tcttccacgg gtgaaaaagc gaatgctttt
    41341 actgccagca atacggaaat aagcagccag ataatatagt gtttaggaac acaacgccga
    41401 cgtttaaaga aagcgatggt caaccacagt gtgtaatacc acatggcgat agcggtaata
    41461 aaagaaacgc cccataagat ttgtgtcgtc agtgcttgtc cgccgagcgt ttgaaatgtt
    41521 tgcggagaag ataacgcagc ggtatacagc aacaacgcca gcgtcgtact caacagagcg
    41581 actaaaagcc aggccaacgg gccaagcaac cagcctccaa tacgttgtgg cgttgtggtg
    41641 gtcataggtt ctccagaata gcgacgcaaa caggcaaatc taattttagg ggcgttatta
    41701 taaggctttt ataaccgatc gctactttct ttattggaca tagtgccaga cggacttcgg
    41761 tacctggccg aggtcaaaca aacgcccgcc agaaaccagc tctgcgcgac ggtgatcggc
    41821 agcacgatac atattgatca gttcctgatc gtccgtcagg gtgtaattaa gatggtcgta
    41881 gagtttttcc agactttcga gtgaactgat tttgcgaaat tttaataagt agtcctgaac
    41941 agtcataaaa ggtccataaa ataattaata ccaaaggggt aggtattatt agtgatgata
    42001 aaatttcacc aaaagtgaac gcttgtgtta ttacaataat aagaatgcgc tgatggcaaa
    42061 tattcaggga tgaaaaactg ctctgaaaag agtaacgcgc tttactatcc ctgacaatca
    42121 ccaacaacat cgaacaagat aataaattcc tggtttaata tccgacaagt gaaaacatgc
    42181 acccggacgg gcagcatggc gctctacaag cgcagagctt acttgtgttg taccgaagca
    42241 ctctgttcag gtggctgata gttgtcaatg tgactcgcca cgccaagaag aatgactgaa
    42301 acgacaagaa cgatccaacc tgttaattca ataagacgat tcattacagc ccacatctct
    42361 cttgattgat ccattaactt cagggggtaa atgttactta gcaataatag ctccagcaag
    42421 atttttactg aggtttttgc gatatttagc ttttgtcgtt ggaaaattcg ctattttttt
    42481 gacttaagtt aaacaacatc ccttattgct ggcaggttat taaactgttg agcgtgggta
    42541 aaggatagtg tcaaatagca atcatacttc aatgagaggc aatgacatga gcgacaacat
    42601 ccgtgttggg ttgattgggt atggttatgc gagcaaaacc ttccatgcgc ccctgattgc
    42661 gggcacgccc gggctggaac tggcggtaat ctccagcagt gatgaaacaa aagtaaaagc
    42721 cgactggcca acggttacgg ttgtctctga gccgaagcat ctgtttaacg atcccaacat
    42781 agacctgatt gtcattccta cacccaacga tacccatttc ccgttagcca aagcggcgct
    42841 tgaggcgggt aaacatgtgg tcgttgataa accctttacc gtgacactgt cacaagcgcg
    42901 agagctggat gcgctggcaa aaagcctggg gcgtgtgctg tctgtattcc ataaccgtcg
    42961 ttgggatagc gatttcttga cgctaaaagg tttgctcgcg gaaggcgtgc tgggtgaagt
    43021 tgcttacttt gagtctcatt ttgaccgctt ccgtccgcag gtgcgcgatc gttggcgtga
    43081 acagggcggc ccaggcagcg gtatctggta cgatttagca ccgcatcttc ttgatcaggc
    43141 cattacgcta tttggtttac cggtcagcat gacggtagat ttggcacagt tacggcccgg
    43201 agcgcagtcg accgattatt tccacgccat cttgtcctat ccacagcggc gagtcatttt
    43261 acacggtacc atgctggcag ccgctgagtc agcacggtat atcgtgcatg gatcccgagg
    43321 cagttatgtg aaatatggcc tcgatccaca ggaagaacgt ctgaaaaatg gcgagcgtct
    43381 gccgcaggaa gactggggct acgatatgcg tgatggcgta cttacccgcg tggaaggtga
    43441 ggaacgtgtc gaagaaacgc tgttgacggt acctgggaat tatccggctt actatgcggc
    43501 tattcgtgat gcgttaaatg gcgatggtga aaatccggtt ccggcaagcc aggcaatcca
    43561 ggtaatggag ttgattgagc tgggcatcga atccgccaaa catcgcgcga ctttgtgcct
    43621 tgcatgaaaa atggatggtg caatttgcac catccatttt gttacttcgc ggcgactttt
    43681 tctcgcagtg cgcgtttttc ctcagcgctg aggaaagcca tttccagacc attaatctgt
    43741 gcctggcgga tttgctcgcg ggataaccca gcagctggcg cggcaacggt atattcgtga
    43801 atgatatcca ctccctgtac gccgggatcg tcagtgttaa tgccggcacg aatgccatgc
    43861 tcaaggaacg ttttcagcgg atgtgcagcc agctctgcta cggtgctggt ctgaatattg
    43921 gaggtcagac aggattcaat accaatttgt tgctcggcga gaaaatccat cagcgcccga
    43981 tcttcaatgg cttttacgcc atgtccaata cgctccgcac ccagttcacg aatcgcctgc
    44041 cagatgcttt ccggcccggc agcttcgcct gcatggacgg taatatgcca gcccgcatca
    44101 cgcgcaaggt tgaagtgaga aaggaacaga cttcccggga aaccaagttc atcaccggct
    44161 aaatcaagtg cggtaatctg gtcacggtgg gctaaaaagg cctccagctc ttgctgacag
    44221 gcggcttcgc cgaaggttcg gctcataatg ccgataagct tcgcctgcac accaaaagtg
    44281 cggcaacctt cacgtacgcc atcgatcacc gcttcgacaa cacccgctac aggcagctta
    44341 tgtgccattg ccatgtagcc tggtgaaaaa cgcagctcga catagtgcag gccgttacgg
    44401 gctgcatctt caatgttttc aaatgccacg cggcggcagg catcaagaga ggcgagaact
    44461 ttaacgcccc agtcaagttt ggtcagaaag ctcaccagat cgggttcgtt ggcaatgacc
    44521 tgaacgtggg gaatcagtgt ttccagggat tgtgcaggaa gcgagatatt atactggcgg
    44581 ccaagttcaa gaatggtctg gggacgaatg ttgccatcaa ggtggcgatg gatatcagtt
    44641 aatggcaggg tggtatcaat catggtcgca ctctttttct ggatataaaa gtgcgcacca
    44701 ttataaaaac aaagcagggt aaaaatccac gattgcgcaa cgatttttta cgttgcgcaa
    44761 ttggggttaa cgaacagcgc ggatggcgtt aattaatcca gccacacctt tttccagttt
    44821 cgaacgtggg cagccggcat tgagacggac aaaaccacga ccttcttcac cgtaggtata
    44881 ccccggcatg atcgcgactc tttcttgttc gataagtgct ttttgcaacg cgttgtcgtc
    44941 aatattcaac ggacgtaaat caagccatgc cagataagtg gattgtggga tctgccagtt
    45001 gagttcagga aacgcggcgt tcattttatc tgcgatatac gtgaggttat ctttcagata
    45061 gatgcgtaag gcatccagcc acggcgcgcc ttgctgatag gcggcgatat gggcagttaa
    45121 cgccagtacc gaaggggaag aaagcccatc acggcctttc agtgccgata aataggcatc
    45181 gcggctactg ctattttcta taatcccgta agcaccggtc agggcgggaa tattgaaact
    45241 tttcgagccc gacgttagca acgcccagtc tccgcgagcc acattactcc agggaatatg
    45301 cggctgctcg ccccaaacca tatccatatg gatttcatcg gaaataaccc gcacaccatg
    45361 acgctcgcac aggtcagcca tgatctccag ctcatcgcac gtccacactt tcccggtagg
    45421 attctgtggg ctacacagga gcataatttt acattctggt ttcgccaaca cggcttccaa
    45481 cttgcccata tcgcaaaacc aaccatcagc ctgcttctct aaagcaacgg gcattactgt
    45541 gcgctggtta ccttcaatgg ccttgtaaaa tgcgtcatag gcgggtgtgt ggatcaccac
    45601 gccttcacct gtttcagacc actgacgaat cagttctgaa accatataga tgacagaagg
    45661 gccatacacc accgtctgag aatcgatggc ggtgtaatgc tgggtggaaa accagtgggc
    45721 aatagccgcg agaaactcat cgtttttcca gcggctgtag ccaaatacgc cgtgcatcag
    45781 gcgctgattc agcgcctcta taatgcaggg ggcagtggca aaatccatgt ctgaaatcgt
    45841 gaacggtaac aggtcagcag tgccgaaacg gtcagcgaca taatcccact gtgtacacca
    45901 tgtgccatga cgatccacga cctttgaaaa atcgaacata tcttatcctt atgcctggac
    45961 agtatgcatc agaccggcca tttcatcttt tactgactga acttgtggcc cgataacaac
    46021 ctgcaggtta tgttgattaa gttgtactac gccaattgcc cgattgtcct tcagtgcctg
    46081 cacattaaca agcgacatat ctttcacaga caaacgcaga cgggtaatgc agttatcgag
    46141 gctgacaata ttgtcggcac cgcctaatgc ttcgaggatt gcaggaacgt tgtaacctga
    46201 tttacccggc gcagcggcaa cggctttttc gattgagctg gcaacttcgc tatcgcgccc
    46261 cggggttttg agattgaagc gggtgatagc gaaacggaag atggcgtagt aaacgacaaa
    46321 ccagattgcc gccaccactg acaccatgta ccacttggtt gacagaccat gcaaaatacc
    46381 gaacaccacg aagtcgatga tattgccgtc ggtattaccg atggtgacgc cgagcacaga
    46441 catgacggtg aagccgaggc cggttaacag cgcgtggatg acatacagaa ctggcgctac
    46501 gaacaggaat tccagcggtt cggtagtgcc gccaacgacg caggcgatca ggccagaaat
    46561 cagcagacct ttaattttat ggcgattttc cgggcgcgcg cagtgataca tagctaacgc
    46621 tgcagctggc agaccgccga gaaacgcagg cattttacct tgcgaaagga aacgcgtggc
    46681 gctttcagaa aaaccgtgag tggtcgggca actcaattgc gcctggaaga tggtcagtgc
    46741 gccgctgacg gtttgaccgc agacttcctg cgtgccgcct gcgtcggtaa agcgaattaa
    46801 tgccaccaga atgtgatgca gaccaaacgg caacagcaga cgttcaccgg taccaaacag
    46861 catcggtccg aaatcacccg cgctgtttat catatggccc aagccgctaa tacccatggc
    46921 gaaaatcggc cagactaatg gaatcaccag gccgacaagg cccatcacca gcgaggagat
    46981 aattgatacg aagcgcgtac cgccgaagaa tgccagcgca tccggcaggc ggatattatg
    47041 gaaacgctca tgcagcatcc agacgataat accggcgatc accgcaccga ggatcccggt
    47101 atcgatcgac tggatcccaa gaatgctctg gatgttattg gctttcagaa ccgcggcatc
    47161 cgtggttggc agaatgcctt tattggtcaa ccagaagttt accgcgaggt tcattaccgc
    47221 ataaccgacg aagccagcga atgccgctac gcctttattt tcgcgtgcca ggcccagcgg
    47281 gatggcgata cagaacatga caggcaggaa actaaaagca aacgagccaa tcttactcat
    47341 ccaggtaaag atagcctgca acacggggtt gcccaggacc gggatcaggg ttatgacatc
    47401 atggctgcta agagaactac caatgccgag cataatgccg cagaacgaca ataatgccac
    47461 gggtaacatg aaggttttgc ctaactgctg gaagaactcc cacagcgtga ctttcggtgc
    47521 tgttttcgcc gtcataaaac gactcctcgt agagaacaat ctggattcag taaattgcgc
    47581 gagaagataa aacgttttat caaattttag tgaggcataa atcacattac gcaacgataa
    47641 tagcgggtat aagataaata aaaggtaaaa cgttttatct gtcacataat cagggagtag
    47701 gtcatctgca tggctaccgc caaaaaaata accattcatg atgttgcgct ggctgcgggc
    47761 gtgtcggtaa gtaccgtttc gcaggtgctt agtggcaaag ggcgaatctc taccgccaca
    47821 ggagaacgcg ttaacgccgc cattgaagag ctgggatttg tgcgcaatcg ccaggcgtcg
    47881 gcgctgcgcg gcgggcaaag cggcgtcatt ggtttgatcg tccgtgattt atctgcgccg
    47941 ttttacgccg aattgacggc cggattgacg gaagctctgg aagcgcaggg acggatggtt
    48001 tttttgcttc acggctgtaa agacggcgag cagctggcac agcggttttc actgttactg
    48061 aatcaggggg tcgatggtgt ggtaattgcc ggggctgcag gaagcagcga tgacctgcga
    48121 cggatggcag aagaaaaagc tatcccggtg attttcgctt cccgtgccag ttatcttgat
    48181 gatgttgata cggttcgccc ggacaacatg caggctgcac agttgttgac ggagcatctc
    48241 attcgcaatg ggcatcagcg gatcgcctgg ctgggagggc aaagttcctc attaacccgg
    48301 gcagaacggg tgggaggcta ttgtgcaact ctactaaaat ttggcctgcc gtttcacagc
    48361 gattgggtgc tggagtgcac atccagccag aagcaagctg cggaagctat cacggcgctt
    48421 ttacgtcata acccgaccat cagtgctgtg gtttgctata acgaaactat tgcgatgggg
    48481 gcatggtttg gtttgctgaa agcagggcgg caaagcgggg aaagcggagt cgatcgttac
    48541 tttgagcaac aagtttcgct ggcggcattt accgatgcga caccaaccac acttgatgat
    48601 atacccgtta cctgggccag cacgcctgcg cgggaacttg gtaccacact tgcggatcgc
    48661 atgatgcaaa aaatcaccca tgaagagacg cattcacgca atcttattat tcccgcccgg
    48721 ctcattgcag cgaaataatc ctctctttat ctgctatact tggtagtgtc ctttcctcaa
    48781 ggttaatgat aaaaaatgtg gcacacagg
//
LOCUS       DAJTMK010000009        44220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTMK010000009 DAJTMK010000000
VERSION     DAJTMK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44220
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.009"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..718
                     /gene="dgt"
                     /locus_tag="OO135_000441"
     CDS             <1..718
                     /gene="dgt"
                     /locus_tag="OO135_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR5396403.1"
                     /translation="DDISYCVADLEDAVEKRICTVEQLYHHLHEAWGQHEKGSLFSLV
                     VENAWEKSRSNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALL
                     EDASECSDLLKLYKNVAVKHVLSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTE
                     LVEKERVKRFPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYI
                     SGMTDLYAWDEYRRLMAVEQ"
     gene            848..2272
                     /gene="degP"
                     /locus_tag="OO135_000442"
     CDS             848..2272
                     /gene="degP"
                     /locus_tag="OO135_000442"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5396404.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2427..3584
                     /gene="cdaR"
                     /locus_tag="OO135_000443"
     CDS             2427..3584
                     /gene="cdaR"
                     /locus_tag="OO135_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR5396405.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNCISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3673..4059)
                     /locus_tag="OO135_000444"
     CDS             complement(3673..4059)
                     /locus_tag="OO135_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5396406.1"
                     /translation="MYDNLKSLGITNPEEINRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4221..5033)
                     /gene="yaeI"
                     /locus_tag="OO135_000445"
     CDS             complement(4221..5033)
                     /gene="yaeI"
                     /locus_tag="OO135_000445"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR5396407.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5088..5912)
                     /gene="dapD"
                     /locus_tag="OO135_000446"
     CDS             complement(5088..5912)
                     /gene="dapD"
                     /locus_tag="OO135_000446"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5396408.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5943..8615)
                     /gene="glnD"
                     /locus_tag="OO135_000447"
     CDS             complement(5943..8615)
                     /gene="glnD"
                     /locus_tag="OO135_000447"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5396409.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGVQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8677..9471)
                     /gene="map"
                     /locus_tag="OO135_000448"
     CDS             complement(8677..9471)
                     /gene="map"
                     /locus_tag="OO135_000448"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5396410.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9839..10564
                     /gene="rpsB"
                     /locus_tag="OO135_000449"
     CDS             9839..10564
                     /gene="rpsB"
                     /locus_tag="OO135_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5396411.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10822..11673
                     /gene="tsf"
                     /locus_tag="OO135_000450"
     CDS             10822..11673
                     /gene="tsf"
                     /locus_tag="OO135_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5396412.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11820..12545
                     /gene="pyrH"
                     /locus_tag="OO135_000451"
     CDS             11820..12545
                     /gene="pyrH"
                     /locus_tag="OO135_000451"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5396413.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12837..13394
                     /gene="frr"
                     /locus_tag="OO135_000452"
     CDS             12837..13394
                     /gene="frr"
                     /locus_tag="OO135_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5396414.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13486..14682
                     /gene="ispC"
                     /locus_tag="OO135_000453"
     CDS             13486..14682
                     /gene="ispC"
                     /locus_tag="OO135_000453"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5396415.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDLCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14871..15629
                     /gene="ispU"
                     /locus_tag="OO135_000454"
                     /gene_synonym="uppS"
     CDS             14871..15629
                     /gene="ispU"
                     /locus_tag="OO135_000454"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5396416.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15642..16499
                     /gene="cdsA"
                     /locus_tag="OO135_000455"
     CDS             15642..16499
                     /gene="cdsA"
                     /locus_tag="OO135_000455"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5396417.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16511..17863
                     /gene="rseP"
                     /locus_tag="OO135_000456"
     CDS             16511..17863
                     /gene="rseP"
                     /locus_tag="OO135_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5396418.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17893..20325
                     /gene="bamA"
                     /locus_tag="OO135_000457"
     CDS             17893..20325
                     /gene="bamA"
                     /locus_tag="OO135_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5396419.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGVVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20447..20932
                     /gene="skp"
                     /locus_tag="OO135_000458"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20447..20932
                     /gene="skp"
                     /locus_tag="OO135_000458"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5396420.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20936..21961
                     /gene="lpxD"
                     /locus_tag="OO135_000459"
     CDS             20936..21961
                     /gene="lpxD"
                     /locus_tag="OO135_000459"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5396421.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22066..22521
                     /gene="fabZ"
                     /locus_tag="OO135_000460"
     CDS             22066..22521
                     /gene="fabZ"
                     /locus_tag="OO135_000460"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5396422.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22525..23313
                     /gene="lpxA"
                     /locus_tag="OO135_000461"
     CDS             22525..23313
                     /gene="lpxA"
                     /locus_tag="OO135_000461"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5396423.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            23313..24461
                     /gene="lpxB"
                     /locus_tag="OO135_000462"
     CDS             23313..24461
                     /gene="lpxB"
                     /locus_tag="OO135_000462"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5396424.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24458..25054
                     /gene="rnhB"
                     /locus_tag="OO135_000463"
     CDS             24458..25054
                     /gene="rnhB"
                     /locus_tag="OO135_000463"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5396425.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25091..28573
                     /gene="dnaE"
                     /locus_tag="OO135_000464"
     CDS             25091..28573
                     /gene="dnaE"
                     /locus_tag="OO135_000464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5396426.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMASKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVYPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28586..29545
                     /gene="accA"
                     /locus_tag="OO135_000465"
     CDS             28586..29545
                     /gene="accA"
                     /locus_tag="OO135_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5396427.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVHLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            29644..31785
                     /gene="ldcC"
                     /locus_tag="OO135_000466"
     CDS             29644..31785
                     /gene="ldcC"
                     /locus_tag="OO135_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5396428.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPVHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     HREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLWAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFEADIHGAKQDEDGVYRVRVLKMAG"
     gene            31842..32231
                     /locus_tag="OO135_000467"
     CDS             31842..32231
                     /locus_tag="OO135_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5396429.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            32296..33594
                     /gene="tilS"
                     /locus_tag="OO135_000468"
     CDS             32296..33594
                     /gene="tilS"
                     /locus_tag="OO135_000468"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5396430.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIMPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(33643..33903)
                     /gene="rof"
                     /locus_tag="OO135_000469"
     CDS             complement(33643..33903)
                     /gene="rof"
                     /locus_tag="OO135_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5396431.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(33890..34090)
                     /locus_tag="OO135_000470"
     CDS             complement(33890..34090)
                     /locus_tag="OO135_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5396432.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            34256..34801
                     /locus_tag="OO135_000471"
     CDS             34256..34801
                     /locus_tag="OO135_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5396433.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAVQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            34798..35160
                     /gene="arfB"
                     /locus_tag="OO135_000472"
     CDS             34798..35160
                     /gene="arfB"
                     /locus_tag="OO135_000472"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5396434.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLA"
     gene            35234..35944
                     /gene="nlpE"
                     /locus_tag="OO135_000473"
                     /gene_synonym="cutF"
     CDS             35234..35944
                     /gene="nlpE"
                     /locus_tag="OO135_000473"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5396435.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(36144..36968)
                     /locus_tag="OO135_000474"
     CDS             complement(36144..36968)
                     /locus_tag="OO135_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5396436.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHIAIFLGDNN
                     VAEATGAGVHIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTR"
     gene            complement(37021..38739)
                     /gene="proS"
                     /locus_tag="OO135_000475"
     CDS             complement(37021..38739)
                     /gene="proS"
                     /locus_tag="OO135_000475"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5396437.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(38850..39557)
                     /gene="tsaA"
                     /locus_tag="OO135_000476"
     CDS             complement(38850..39557)
                     /gene="tsaA"
                     /locus_tag="OO135_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5396438.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(39554..39958)
                     /gene="rcsF"
                     /locus_tag="OO135_000477"
     CDS             complement(39554..39958)
                     /gene="rcsF"
                     /locus_tag="OO135_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5396439.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(40076..40891)
                     /gene="metQ"
                     /locus_tag="OO135_000478"
     CDS             complement(40076..40891)
                     /gene="metQ"
                     /locus_tag="OO135_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5396440.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(40931..41584)
                     /gene="metI"
                     /locus_tag="OO135_000479"
     CDS             complement(40931..41584)
                     /gene="metI"
                     /locus_tag="OO135_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5396441.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(41577..42608)
                     /gene="metN"
                     /locus_tag="OO135_000480"
     CDS             complement(41577..42608)
                     /gene="metN"
                     /locus_tag="OO135_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5396442.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            42796..43368
                     /gene="gmhB"
                     /locus_tag="OO135_000481"
     CDS             42796..43368
                     /gene="gmhB"
                     /locus_tag="OO135_000481"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5396443.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFTDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            43732..>44220
                     /locus_tag="OO135_000482"
     rRNA            43732..>44220
                     /locus_tag="OO135_000482"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        10762 a        10813 c        12049 g        10596 t
ORIGIN      
        1 cgacgacatc tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatgtac
       61 cgttgagcag ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct
      121 cttttcgctg gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag
      181 tacggaagat cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta
      241 tgcggcacaa cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc
      301 attattggaa gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt
      361 aaaacatgtg cttagccatc cagatgtcga gcagcttgaa ttgcagggct atcgggtcat
      421 tagcggatta ttagagattt atcgtccttt attaagcctg tcgttatccg actttactga
      481 actggtagaa aaagaacggg tgaaacgttt ccctattgaa tcgcgcttat tccacaaact
      541 ctcgacgcgc catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc
      601 tgagtttccg ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg
      661 tatgaccgac ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc
      721 aggcttttgt aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact
      781 tcgcgctata aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact
      841 gaaatacatg aaaaaaacca cattagcact gagtgcactg gctctgagtt taggtttggc
      901 gttatctccg ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat
      961 gccaagcctt gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga
     1021 aggtagcaca accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga
     1081 tgattctccg ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggtgg
     1141 ccagggcggt aatggtggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat
     1201 cattgatgcc gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt
     1261 gattaaagtc cagctgagcg atggccgtaa gttcgacgcg aagatggtcg gcaaagatcc
     1321 gcgctctgat atcgcgctga tccaaatcca gaacccgaaa aacctgaccg caattaagat
     1381 ggcggattct gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg
     1441 tctgggcgag acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc
     1501 tgaaaactac gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg
     1561 tgcgctggtt aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga
     1621 cggcggcaac atcggtatcg gttttgcaat cccgagcaac atggtgaaaa acctgacctc
     1681 gcagatggtg gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct
     1741 gaactccgaa ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca
     1801 ggttctgcct aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc
     1861 actgaacggt aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc
     1921 ggtgggcagc aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct
     1981 agaactgcag cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga
     2041 aggcgctgag atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaaaac
     2101 gggcactccg gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gcgcgaacca
     2161 gcaggcagtg aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct
     2221 ggcactgaac attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctcactc
     2281 aaccccttcc tgaaaacggg aaggggttct ccttacaatc tgtgaacttc accacaactc
     2341 catacatctt catcatcctt taggcatttg cacaatgccg tacgttacgt acttccttat
     2401 gctaagccgt gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc
     2461 aggatatcgt ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc
     2521 gtgggcgaat tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat
     2581 tgctggtact ttcacaggga cgagtcgtcg atatcgatga cgcggtggca cgtcatctgc
     2641 acggtgtgcg gcaagggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa
     2701 ttggcctgac aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg
     2761 ctgaaatgat gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc
     2821 gggaggaact ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttactgaat
     2881 gggcgcaacg gctggggatc gatctcaatc aaccgcgagt ggtggctatt gttgaggtcg
     2941 acagcggtca gcttggtgtg gacagcgcaa tggcggagtt acaacaactg caaaacgcgc
     3001 tgactacgcc cgagcgtaat aatctggtgg cgattgtctc gctaaccgaa atggtggtgt
     3061 tgaaaccggc gttgaactct tttgggcgct gggatgcaga agatcatcgt aagcgagttg
     3121 aacaactgat tacccgcatg aaagagtacg gccagctgcg ttttcgcgtt tcactgggca
     3181 actattttac cggtcctggc agtattgccc gatcctatcg tacggcgaaa acgacgatgg
     3241 tggtgggtaa acagcggatg ccagaaagtc gctgctattt ttatcaggat ctgatgttac
     3301 ctgtgttact cgacagtttg cgtggcgact ggcaggccaa cgaactggcg cgaccgctgg
     3361 cgcggctgaa agcgatggac aataacggct tgctgcgacg aacgctggcg gcgtggtttc
     3421 gtcacaatgt gcaaccgctg gcaacgtcaa aggcgttgtt tattcatcgt aataccctgg
     3481 agtatcggct taattgtata tcggaactga ccgggcttga tttgggcaat tttgatgaca
     3541 ggttgctgct gtatgtggcg ttacaactgg atgaagagcg gtaggttatg cgttaaggtt
     3601 gaggcggcgc tgtctcattc ccctcagccc gaaggggaga ggggaatgcc cgaattaacc
     3661 gtcaattcgg gattatttac gcgttagttt ttccagatcg gcttcaatct cgctgatctt
     3721 attggttacg actgactcca ggtgacgtaa gtcgtcgagg atcttacgct taagatcaac
     3781 ttcgctgcgg tcacgctggc agatttgatc aagctcatca atgatatacc gtagattcgg
     3841 gctgatttcc tggacttctt tataaccctg acccacacca tcagcgacga ccgttttacg
     3901 ctgacgcgga tatttaaact taacgctctt ggcgaaaaac tcgcctttgt ctttctggaa
     3961 atagattttc agaatatcgt tgttggcttc ctgccggagg ctgtaacgat taatttcttc
     4021 aggattggta atacccagac ttttcagatt gtcgtacata gcgttacctc aaaatgagtc
     4081 agtaaattgt gcttatttta gcatttggcc tggcccgccc ggctcgactg tgatcgcaga
     4141 aagccttttt cttccagact tctatcatgg cgtaaaaatc aaaaaattac ctgctttatt
     4201 gtggtgataa aattcacgtt ctacaccagt tccagcatcg ttacttccgg gcggcagttc
     4261 agacgcaaac catacaaact gcccacgcca cgggttgtgt agatgtgtcg ttcgccaaag
     4321 gcatttaatc cggcgacgta acgtttatct tcgacagggg caaaaggttc accaaccaac
     4381 ggtacgcgca gttgcccgcc gtgagtatgg ccgcacagca tcagatccca gggttcatca
     4441 cgcatgactt ctttgctgtc gggattatgt gccagcatca gtcttggcag atttgcttcg
     4501 ctggcgggag gtggtttgca ttgtccggcc cataagtcac cggtgcctac cagttcgaat
     4561 tgcctgttcg gcgtggcgat caccgtagcc tggttaaaca acaccgtgat gcccactgat
     4621 ttcaacgtct cgccaattaa gtgatttttt tctgtaccaa cagggcgatc gtggttgccg
     4681 aagcaggcaa acgtcggcgc acattcggca aggggggaga ggacatcact aaacgccgaa
     4741 aaattcagcg gcatatcaaa taatacgtaa tcgccgccca gtaatatcaa atcgggcttt
     4801 tgttctatgc caagagcaat cgcgtcagaa atcaggctta aaggaacaaa acgagagtaa
     4861 tggagatcgg ccagaaaaag aattttgaat ggtgctgcgt tgtctttaaa aaaggcgagg
     4921 cggtgacgga ttaattcaaa ccagcctggc tcccagtaat gcatataacc gaaacctgag
     4981 ctcgtggcta tcgtcgcggc agtagcctgc aacaatcggc ggcgtgaaat catcgctcat
     5041 ccctgcaaaa aaaatcgggc agcgtcgtgc tgcccgtgtg catactttta gtcgatggta
     5101 cgcagcagtt cgttaatgcc gactttgccg cgagttttcg cgtcaacttt cttaacgata
     5161 accgcacagt agaggctgta tttgccatct tttgacggca gattacctga aacaaccaca
     5221 gaccccgccg gaacgcgacc gtagtggatt tcgccggttt cacggtcgta aatacgggtg
     5281 ctctgaccaa tgtatacgcc catggaaatg acggaacctt cttcgacaat caccccttca
     5341 actacttcag agcgtgcgcc aatgaagcag ttatcttcaa tgatggttgg gttagcctgc
     5401 agcggttcca gcacgccgcc gatgcccacg ccaccggaaa ggtggacgtt tttaccaatc
     5461 tgcgcacaag aaccgacggt cgcccaggta tcaaccatgg tgccttcatc aacatatgcg
     5521 ccgatgttga cgtaagacgg catcagcacg gtgttacggg caataaacgc accctgacgt
     5581 accgccgctg gtggcacaac gcggaagcct tctttctgga aacgtgcttc gtcgtagtcg
     5641 gcgaatttca tcggcacttt gtcgaagtag cggctttctg ccccttcgat cacctgatta
     5701 tcattaatac ggaaagagag cagcaccgct tttttcaacc actgatgcgt cacccactga
     5761 ccgtcaattt tttccgctac acgcagtgca ccggaatcca gcagggcgat cacctgattt
     5821 accgcttcgc gggtaacggt gtctgcgttg gctggcgtga tctcggcacg gcgttcaaaa
     5881 gcggtttcaa taatgttctg taactgctgc attgttaaac tcttttcata tcagtaaaca
     5941 catcaccctt tatcgtttgg attgagggcc tctgtcaacc gctgatgcac ttcctgctgc
     6001 aactcgttat taagcgcacg ccggtcagcg gtggcaatta tgaataaatc ttctactcgc
     6061 tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc ccagatcggc aaaaattttc
     6121 ccgactctcg ccagcagtcc tggctggtcg agcgcgataa gctcaaggaa cgatttgcgg
     6181 tcggtatggg tcggcaaaaa cgttacttcg gtttcaacag taaaatggcg taatttggcg
     6241 ggttggcgac ggggctgcgg tggctgccag ctactttgcg tcagtacttg ctccagacca
     6301 aaccgaataa cctcatgacg atctgcggac agcgggctgc catcgggttc cagcacgata
     6361 aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt gtgcgtcgtg aacacttaaa
     6421 ttgcggcggt ctaattcggc acagacggcg gcaaacagat aggggcggtc cgggctccag
     6481 ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa gcaataccag tggtttgctt
     6541 aaatcatgct gtaataaatg gcgggcatgc caggccagtt gatttgggct atggcggaca
     6601 aaatagttag cacgacagcg tgaccaaatt tggtgcagcg cctcttcgtc gatgttatcc
     6661 atgcgcagta gtgccagtgc ctggagttgg tgatggcgaa cccgttcgcg catatccggc
     6721 gtgttttgca ttccgcgtcg tagctgcttt tcggtggcaa aatagagttc gcgcaacaga
     6781 ctttgcttcc agctattcca cagcgtttcg ttggtggcgc aaatgtcagc cactgtcagg
     6841 cataccagat agtgcagacg attttccgtt tgaacttctt cggcaaactg cttgatgact
     6901 tcagggtcct gaatatcgcg gcgttgtgct gtcaccgaca tcagcaagtg ctggcgaacc
     6961 agccaggcaa ccagctgtgt ttcgcgtgag ttcagcccgt ggagttctgc aaaatgcact
     7021 acatcctgaa caccgagaat ggagtggtcg ccgccgcgtc ctttggcgat atcgtgaaac
     7081 agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg gccagacgtc cacacacaac
     7141 ggatggcgct ggcgcgtttc ttcactggca aaactctcca gtttcagcat cacgcggata
     7201 gtatgttcat ccaccgtgta ggcgtggaac agatcaaact gcatctgccc gacgatatgc
     7261 gaccattgcg gcatatacgc gccgagcacg ctatggcgat gcattggcaa tagcccgcgc
     7321 cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt ttcgtgcttc cggaatatta
     7381 cacagcggtt gttgcagatg gcgacgggca tggcgtaact ggcgcagcgt ggtggagtaa
     7441 atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca tacgcaagat ggcttccggc
     7501 tggcgcataa atagtgtttc atcacgcagg tcgattagcg taccgcgtag ctgaaactca
     7561 tcgtcgattg gacgtggttt ttcgtcggca ggaagggcga ggatggcttc atcgaacagt
     7621 tgcagcagca tctggttgag ttcactgacg cggcgtgtaa cgcggaagta atccttcatc
     7681 atccgctcga ccggttcgtt gccttcacca ctgtaattca gacgctgagc gacgctaagt
     7741 tggcgatcga ataacaggcg attatcgtaa cggctgacga ccagatgcag ggcaaagcga
     7801 atacgccaca gtatatgcag acattcgttt aattccgccc gctccgctga ggttaagaag
     7861 ccaaacccaa ccatttcatc cagcgatgtt gcgccaaaat gacggcgggc cacccattgc
     7921 agagtgtgga tatcgcgcaa gccgccaggg ctacttttga tgtctggttc aagattgtag
     7981 ctggtgccgt ggtaacgctg atggcgctgg ttctgttctt caactttcgc cgcgtagaac
     8041 ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt gcagttcgag gaagagcgca
     8101 acatcgccaa ttaataagcg ggattcgatt aaattggtgg cgacggttaa atccgataac
     8161 ccttccagca tgcactcttc aagcgtgcgc acactatgac cgacttccag ctttacatcc
     8221 cagagcagcg ttaacagctc gcccactttt tgcgcctgat cgtccgggag ctttttacgg
     8281 cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct cgccacgacc gtagccaccg
     8341 acggcgacca atgccaggtc ggcaatctgg ctgaatcccg cttcaatcca taatcgttgc
     8401 aggagctggt cgataaactc ggtgcgcgcc tcaatcaact gttctgcaga gatcccattg
     8461 tcaaaggcat cacccagcca acgctggaaa gtatcgatat gggcttttat cccaccgacg
     8521 gttaattcat cacggggcca gacgcatgga ttttgcggtt gaccgggcag ggtggggaga
     8581 gcggtgtttg cgtactgttc tggaagggta ttcattgtgc gccactcata agattaaatt
     8641 atcgcattaa aaaagccggc attcgccggc ttcatcttat tcgtcgtgcg agattatcgc
     8701 cgggatggtg tcatccttgc gtagcgtcag aatttcgcag ccgttatcag tcaccacaat
     8761 agtatgctca tattgtgcag acaagctgcg atctttggtt tttaccgtcc agccatcttt
     8821 catggtgcgg atctcttttt tacccgcgtt gaccattggc tcgatggtga acgtcatccc
     8881 aggtttcagt acgacgttgg tttcacggga gtcatagtgc agcacctgcg gttcttcatg
     8941 gaagccgcga ccaataccgt gtccgcaata ttcacgaacg acggagaagc cttctgcttc
     9001 gacaaacttc tgaatcgccg caccgatttc gcgcaggtta atgcctggtt tgaccatgcg
     9061 cagcgccagg tacaggcttt cttgcgtgat gcggcacaga cgttcgccca tgatggtcgg
     9121 cttaccgacg ataaacattt tcgaggtatc gccgtggaaa ccatctttga ttacggtgac
     9181 atcaatgtta acgatatcgc catctttcag cagcttcgca tcgtccggga taccgtggca
     9241 caccacttca ttaatagaga tgcaaacgga tttcggatag ccgtgatagc cgaggcaggc
     9301 agaaaccgcg tgttgttcat taacaatgta atcattacag atgcgatcca gctcgccggt
     9361 gctgacgccc ggtttaacat acggttcgat catctccagc acttcggcag ccagtcggcc
     9421 agcgacgcgc attttttcga tatcttctgg ggtcttgatt gagatagcca ttaattctgt
     9481 ccatcagcgt cggtgatacc gacaatatat atgtaagtgc cgtcaatggt atcacacccg
     9541 ggcaaattga gaatcattct gaatttcgcc aaacgtgcca ctgaacgttt tctataatag
     9601 aaaattcaac gtctgatgct gtacacagcg ccaacaatta ttggtgtcca tgacgtattt
     9661 gtggtataaa gcgcgccgga cttccgatcc atttcgtata cacagactgg acggaagcga
     9721 caatctcact ttgtgtaaca acacacacgt atcggcacat attccggggt gccctttggg
     9781 gtcggtaata tgggatacgt ggaggcataa ccccaacttt tatatagagg ttttaatcat
     9841 ggcaactgtt tccatgcgcg atatgctcaa ggctggtgtt cacttcggtc accagacccg
     9901 ttactggaac ccgaaaatga agccgttcat cttcggtgcg cgtaacaaag ttcacatcat
     9961 caaccttgag aaaactgtac cgatgttcaa cgaagctctg gctgaactga acaagattgc
    10021 ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc gctgcaagcg aagcggtgaa
    10081 agacgctgct ctgagctgcg accagttctt cgtgaaccat cgctggctgg gcggtatgct
    10141 gactaactgg aaaaccgttc gtcagtccat caaacgtctg aaagacctgg aaactcagtc
    10201 tcaggacggt actttcgaaa agctgaccaa gaaagaagcg ctgatgcgca ctcgtgagct
    10261 ggagaaactg gaaaacagcc tgggcggtat caaagacatg ggcggtctgc cggacgctct
    10321 gtttgtaatc gatgctgacc acgaacacat tgctatcaaa gaagcaaaca acctgggtat
    10381 tccggtattt gctatcgttg ataccaactc tgatccggac ggtgttgact tcgttatccc
    10441 gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg ggcgctgttg ctgcaaccgt
    10501 acgtgaaggc cgttctcagg atctggcttc ccaggcggaa gaaagcttcg tagaagctga
    10561 gtaataaggc ttgataactc ccccaaaata gttcgagttg cagaaaggcg gcaagctcga
    10621 gaattcccgg gagcttacat cagtaagtga ccgggatgag cgagcgaaga taacgcatct
    10681 gcggcgcgaa atatgaaggg ggagagccct tatagaccag gtagtacacg tttggttagg
    10741 gggcctgcat atggccccct ttttcacttt tatatctgtg cggtttaatg ccgggcagat
    10801 cacatctccg aggattttag aatggctgaa attaccgcat ccctggtaaa agagctgcgt
    10861 gagcgtactg gcgcaggcat gatggattgc aaaaaagcac tgactgaagc taacggcgac
    10921 atcgagctgg caatcgaaaa catgcgtaag tccggtgcta ttaaagcagc gaaaaaagca
    10981 ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg acggcaacta cggcatcatt
    11041 ctggaagtta actgccagac tgacttcgtt gcaaaagacg ctggtttcca ggcgttcgca
    11101 gacaaagttc tggacgcagc tgttgctggc aaaatcactg acgttgaagt tctgaaagca
    11161 cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg gtgaaaacat caacattcgc
    11221 cgcgttgctg cgctggaagg cgacgttctg ggttcttatc agcacggtgc gcgtatcggc
    11281 gttctggttg ctgctaaagg cgctgacgaa gagctggtta aacacatcgc tatgcacgtt
    11341 gctgcaagca agccagaatt catcaaacca gaagacgtat ccgctgaagt ggtagaaaaa
    11401 gaataccagg tacagctgga tatcgcgatg cagtctggta agccgaaaga aatcgcagag
    11461 aaaatggttg aaggccgcat gaagaaattc accggcgaag tttctctgac cggtcagccg
    11521 ttcgttatgg aaccaagcaa aactgttggt cagctgctga aagagcataa cgctgaagtg
    11581 actggcttca tccgcttcga agtgggtgaa ggcatcgaga aagttgagac tgactttgca
    11641 gcagaagttg ctgcgatgtc caagcagtct taattatcaa aaaggagccg cctgagggcg
    11701 gcttcttttt gtgcccatct tgtaaattca gctaaccctt gtggggctgc gctgaaaagc
    11761 gacgtacaat gtcgctagta ttaattcatt tcaatcgttg acagtctcag gaaagaaaca
    11821 tggctaccaa tgcaaaaccc gtctataaac gcattctgct taagttgagt ggcgaagctc
    11881 tgcagggcac tgaaggcttc ggtattgatg caagcatact ggatcgtatg gctcaggaaa
    11941 tcaaagaact ggttgaactg ggtattcagg ttggtgtggt gattggtggg ggtaacctgt
    12001 tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt tgtgggcgac cacatgggga
    12061 tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc actgcaccgc gcctatgtga
    12121 acgctcgtct gatgtccgct attccattga atggcgtgtg cgacagctac agctgggcag
    12181 aagctatcag cctgttgcgc aacaaccgtg tggtgatcct ctccgccggt acaggtaacc
    12241 cgttctttac caccgactca gcagcttgcc tgcgtggtat cgaaattgaa gccgatgtgg
    12301 tgctgaaagc aaccaaagtt gacggcgtgt ttaccgctga tccggcgaaa gatccaaccg
    12361 caaccatgta cgagcaactg acttacagcg aagtgctgga aaaagagctg aaagtcatgg
    12421 acctggcggc cttcacgctg gctcgtgacc ataaattacc gattcgtgtt ttcaatatga
    12481 acaaaccggg tgcgctgcgc cgtgtggtaa tgggtgaaaa agaagggact ttaatcacgg
    12541 aataattccc gtgatggata aataagggta agattccgcg taagtatcgc gggggcgtaa
    12601 gtctggttat aaggcgttat tgttgcaggc agtttggtca cggccagcgc gcagcaaccg
    12661 gagcgtacaa aagtacgtga ggatggcgag cactgctcgg ggccaaaatg gcaaataaaa
    12721 tagcctaata atccagacga ttacccgtaa tatgtttaat cagggctata cttagcacac
    12781 ttccactgtg tgtgactgtc tggtctgact gagacaagtt ttcaaggatt cgtaacgtga
    12841 ttagcgatat cagaaaagat gctgaagtac gcatggacaa atgcgtagaa gcgttcaaaa
    12901 cccaaatcag caaaatacgc acgggtcgtg cttctcccag cctgctggat ggcattgtcg
    12961 tggaatatta cggcacgccg acgccgctgc gtcagctggc aagcgtaacg gtagaagatt
    13021 cccgtactct gaaaatcaac gtgtttgatc gttcaatgtc tccggccgtt gaaaaagcga
    13081 ttatggcgtc cgatcttggc ctgaacccga actctgcggg tagcgacatc cgtgttccgc
    13141 tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa aatcgttcgt ggtgaagcag
    13201 aacaagcgcg tgttgcagta cgtaacgtgc gtcgtgacgc gaacgacaaa gtgaaagcac
    13261 tgttgaaaga taaagagatc agcgaagacg acgatcgccg ttctcaggac gatgtacaga
    13321 aactgactga tgctgcaatc aagaaaattg aagcggcgct ggcagacaaa gaagcagaac
    13381 tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc agtagatcct tgcggatcgg
    13441 ctggcggcgt tttgcttttt attctgtctc aactctggat gtttcatgaa gcaactcacc
    13501 attctgggct cgaccggctc gattggttgc agcacgctgg acgtggtgcg ccataatccc
    13561 gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg tcactcgcat ggtagaacag
    13621 tgcctggaat tctctccccg ctatgccgta atggacgatg aagcgagtgc gaaacttctt
    13681 aaaacgatgc tacagcaaca gggtagccgc accgaagtct taagtgggca acaagccgct
    13741 tgcgatatgg cagcgcttga ggatgttgat caggtgatgg cagccattgt tggcgctgct
    13801 gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa ccattttgct ggccaataaa
    13861 gaatcactgg ttacctgcgg acgtctgttt atggacgccg taaagcagag caaagcgcaa
    13921 ttgttaccgg tcgatagcga acataacgcc atttttcaga gtttaccgca acctatccag
    13981 cataatctgg gatacgctga ccttgagcaa aatggcgtgg tgtccatttt acttaccggg
    14041 tctggtggcc ctttccgtga gacgccattg cgcgatttgg caacaatgac gccggatcaa
    14101 gcctgccgtc atccgaactg gtcgatgggg cgtaaaattt ctgtcgattc ggctaccatg
    14161 atgaacaaag gtctggaata cattgaagcg cgttggctgt ttaacgccag cgccagccag
    14221 atggaagtgc tgattcaccc gcagtcagtg attcactcaa tggtgcgcta tcaggacggc
    14281 agtgttctgg cgcagctggg ggaaccggat atgcgtacgc caattgccca caccatggca
    14341 tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatc tttgcaaact aagtgcgttg
    14401 acatttgccg caccggatta tgatcgttat ccatgcctga aactggcgat ggaggcgttc
    14461 gaacaaggtc aggcagcgac gacagcattg aatgccgcaa acgaaatcac cgttgctgct
    14521 tttcttgcgc aacaaatccg ctttacggat atcgctgcgt tgaatttatc ggtactggaa
    14581 aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt tatctgttga tgcgaacgcg
    14641 cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct gaggataatc cggctacaga
    14701 gagtctcgct atttgttagc gtagggcttc agtgatatag tctgcgccat ctgatcgtaa
    14761 gtagttggct ttataaggtc aggtatgccg tggttttgca cggctttttt ttgtataggc
    14821 ttcagtattc ctgagtaccg taaaccctgt cagggaataa aaaacgcgtg atgttgtctg
    14881 ctactcaacc acttagcgaa aaattgccag cgcatggctg ccgtcatgtt gcgatcatta
    14941 tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat tcgtgccttt gggcataaag
    15001 ccggggcaaa atccgtccgc cgggctgtct cttttgcggc caacaacggt attgaggcgt
    15061 taacgctgta tgcctttagt agtgaaaact ggaaccgacc agcgcaggaa gtcagtgcgt
    15121 taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa aagtctgcac cgacataacg
    15181 tgcgtctgcg tattattggc gataccagtc gctttaactc gcgtttgcaa gaatgtattc
    15241 gtaaatctga agcgctaaca gccgggaata ccggtctgac gctgaatatt gcggcgaact
    15301 acggtggacg ttgggatata gtccagggag tcaggcaact ggctgaaaag gtgcagcaag
    15361 gaaacctgca accagatcag atagatgaag agatgctaaa ccagcatgtc tgtatgcatg
    15421 aactggcccc tgtagattta gtaattagga ctggggggga gcatcgcatt agtaactttt
    15481 tgctttggca aattgcctat gccgaacttt actttacaga tgttctctgg cccgatttcg
    15541 atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa tcgagagcgt cgtttcggcg
    15601 gcaccgagcc cggtgatgaa acagcctgat ggggggcgct tttgctgaag tatcgcctga
    15661 tatctgcttt tgtgttaata cccgtcgtca tcgcggcgtt gtttctgttg ccgccggtgg
    15721 ggttcgccat tgtaacgctg gtggtctgca tgctggcagc gtgggaatgg ggacagctta
    15781 gcggttttac cactcgttcg cagcgagtat ggttggcggt gttatgcggg ttattgttgg
    15841 cgctgatgct ttttctgttg ccggaatatc accgaaatat tcatcaaccg ctggttgaaa
    15901 tctcactttg ggcttcgctg ggttggtgga ttgtcgcgct attgctggtg ctgttttacc
    15961 caggttccgc agcaatctgg cgtaactcta aaacattgcg ccttattttt ggcgtgctaa
    16021 ccattgttcc cttcttctgg ggcatgctgg cgttacgggc ctggcactat gacgagaatc
    16081 attacagtgg cgcaatatgg ctgctctatg tcatgatcct ggtatggggc gctgactccg
    16141 gcgcatatat gtttggcaaa ttgtttggta aacataagct ggcaccgaag gtttctccgg
    16201 gtaaaacctg gcaaggcttt ataggtggac tcgctactgc agcggtaatc tcatggggtt
    16261 atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt actcatttgc tctattgtcg
    16321 cagcgttagc ctcagtgctc ggcgatctga ccgagagtat gtttaagcgt gaagcaggaa
    16381 ttaaggacag cggtcattta attccaggac acggtggtat tttagatcgt attgatagcc
    16441 tgacggctgc ggtaccggtc tttgcttgct tgttgttact ggtattcagg acgctttaac
    16501 ggaaggtaat atgctgagtt ttctctggga tttggcttcg ttcatcgttg cactgggtgt
    16561 acttatcacc gtgcatgaat ttggtcattt ctgggttgcc cggcgttgtg gtgttcgcgt
    16621 tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg cgaactgata agctcggcac
    16681 cgaatatgtt atcgccctga tcccgctggg cggttatgtc aaaatgctgg atgagcgcgc
    16741 agaaccggtc gttccggaac tccgccacca tgccttcaat aataaatctg ttggccaacg
    16801 agcggcgatt attgccgcag gtccggttgc aaacttcatt tttgctatct ttgcctactg
    16861 gctggttttt attattggtg tgcctggcgt acgtccggtg gttggtgaaa tagcagccaa
    16921 ttcgatagct gcggaagcac aaattgcacc aggtacggaa ctaaaagccg tagatggtat
    16981 cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc gataaaattg gcgatgaaag
    17041 caccaccatt acagtagcgc catttggcag cgaccaacgg cgggatgtaa agctcgattt
    17101 acgtcactgg gcgtttgagc ctgataaaga agatccggta tcttcgctgg ggattcgtcc
    17161 tcgtgggctg caaattgaac ctgtactgga aaatgtgcag ccaaactcgg cggcaagcaa
    17221 ggcaggtttg caagcaggcg acaggatcgt taaagtcgat ggtcagccct taacgcagtg
    17281 ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa tccttagcgt tagaaatcga
    17341 aaggcaggga agtcccttgt ctttgacatt aatcccggag agtaaaccgg gtaatggtaa
    17401 agcgattggt tttgtcggta ttgagccgaa agtcattcct ttgccagatg agtataaagt
    17461 tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc acggacaaaa cgtggcagct
    17521 gatgaagctg acggtcagta tgctgggaaa attgatcacc ggtgatgtga aactgaacaa
    17581 cctcagtggg ccgatctcta tcgccaaggg ggctgggatg acagcggaac tcggggtagt
    17641 ttattacctg ccgtttcttg cgcttattag cgtgaactta gggataatta acctgtttcc
    17701 gttgcccgta cttgacgggg cgcatctgct gttccttgcg atcgaaaaga tcaagggcgg
    17761 accggtatcc gagcgggttc aagacttttg ttatcgcatt ggctcgattc tgctggtgct
    17821 gttaatgggg cttgcacttt tcaatgattt ctctcggtta tgagagttag ttaggaagaa
    17881 cgcataataa cgatggcgat gaaaaagttg ctcatagcgt cgctgctgtt tagcagcgcc
    17941 accgtatacg gtgctgaagg ggtcgtagtg aaagatattc atttcgaagg ccttcagcgt
    18001 gtcgccgttg gtgcggccct cctcagtatg ccggtgcgta caggcgacac ggttaatgat
    18061 gaagatatca gtaataccat tcgcgctctg tttgctaccg gcaactttga ggatgttcgc
    18121 gtccttcgtg atggtgatac ccttctggtt caggtaaaag aacgtccgac cattgccagc
    18181 attactttct ccggtaacaa atcggtgaaa gatgacatgc tgaagcaaaa cctcgaggct
    18241 tctggtgtgc gtgtgggcga atccctcgat cgcaccacca ttgccgatat cgagaaaggt
    18301 ctggaagact tctactacag cgtcggtaaa tatagcgcca gcgtaaaagc tgtcgtgacc
    18361 ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc aggaaggtgt gtcagctgaa
    18421 atccagcaaa ttaacattgt tggtaaccat gctttcacca ccgacgaact gatctctcat
    18481 ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag gcgatcgtaa ataccagaaa
    18541 cagaaactgg cgggcgacct tgaaaccctg cgcagctact atctggatcg cggttatgcc
    18601 cgtttcaaca tcgactctac ccaggtcagt ctgacgccag ataaaaaagg tatttacgtc
    18661 acggtgaaca ttaccgaagg cgatcagtac aagctttctg gcgttgaagt gagcggcaac
    18721 cttgccgggc actccgctga aattgagcag ctgactaaga tcgagccggg cgagctgtat
    18781 aacggcacca aagtgaccaa gatggaagat gacatcaaaa agcttctcgg tcgctatggt
    18841 tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg atgccgacaa aaccgttaaa
    18901 ttacgcgtga acgttgatgc gggtaaccgt ttctacgtgc gtaagatccg ttttgaaggt
    18961 aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc gtcagatgga aggtgcatgg
    19021 ctgggaagcg atctggtcga tcagggtaag gagcgtctga atcgtctggg cttctttgaa
    19081 actgtcgata ccgataccca acgtgttccg ggtagcccgg accaagtaga tgtagtctac
    19141 aaggtgaaag aacgtaacac cggtagcttc aacttcggga ttggttacgg tactgaaagt
    19201 ggcgtgagct tccaggctgg cgtgcaacag gataactggt taggtacagg ttatgctgtt
    19261 ggtatcaacg ggaccaaaaa cgattaccag acctatgctg aattgtcggt aaccaacccg
    19321 tacttcactg tagatggtgt aagcctcggt ggtcgtctct tctataatga cttccaggca
    19381 gatgacgccg acctgtccga ctataccaac aagagttatg gtacagacgt gacgttgggc
    19441 ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg gttatgtaca taactccctg
    19501 tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt actctatggg tgaacatccg
    19561 agcacctctg atcaggataa cagcttcaaa acggacgact tcacgttcaa ctatggttgg
    19621 acctataaca agcttgaccg tggttacttc ccgacagatg gttcacgtgt caacctgacc
    19681 ggtaaagtga ccattcctgg atcggataac gaatactaca aagtgacgtt agacacggcg
    19741 acttatgtgc cgatcgatga cgatcacaaa tgggttgttc tggggcgtac ccgctggggt
    19801 tatggtgatg gtttaggcgg caaagagatg ccgttctacg agaacttcta tgccggtggt
    19861 tccagcaccg tccgtggctt ccagtccaat accattggtc cgaaagcagt ttacttcccg
    19921 catcaggcca gtaattatga tccggactat gattacgaat gtgcgactca ggacggcgcg
    19981 aaagacctgt gtaaatcgga tgatgctgta ggcggtaacg ccatggcggt tgccagcctc
    20041 gagttcatca ccccgacgcc gtttattagc gataagtatg ctaactcggt tcgtacttcc
    20101 ttcttctggg atatgggtac cgtttgggat acaaactggg attccagcca atattctgga
    20161 tatccggact atagtgatcc aagcaatatc cgtatgtctg cgggtatcgc attacaatgg
    20221 atgtccccat tggggccgtt ggtgttctcc tacgcccagc cgttcaaaaa gtacgatgga
    20281 gacaaggcag aacagttcca gtttaacatc ggtaaaacct ggtaagtgtt ctccacaaag
    20341 gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat ataagatcgc cgggccacgc
    20401 aaagaactgc accctccggt gcaaatggga tggtaaggag tttattgtga aaaagtggtt
    20461 attagcggca ggtctcggtt tagcactggc aacttctgct caggcggctg acaaaattgc
    20521 aatcgtcaat atgggcagcc tgttccagca ggtagcgcag aaaaccggtg tttctaacac
    20581 gctggaaaat gagttcaaag gccgtgccag cgaactgcag cgtatggaaa ccgatctgca
    20641 ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc gatcgcacta agctggaaaa
    20701 agacgtgatg gctcagcgcc agacttttgc tcagaaagcg caggcttttg agcaggatcg
    20761 cgcacgtcgt tccaacgaag aacgcggcaa actggttact cgtatccaga ctgctgtgaa
    20821 atccgttgcc aacagccagg atatcgatct ggttgttgat gcaaacgccg ttgcttacaa
    20881 cagcagcgat gtaaaagaca tcactgccga cgtactgaaa caggttaaat aagtaatgcc
    20941 ttcaattcga ctggctgatt tagcgcagca gttggatgca gaactacacg gtgatggcga
    21001 tatcgtcatc accggcgttg cgtccatgca atctgcacaa acaggtcaca ttacgttcat
    21061 ggttaaccca aaataccgtg agcatttagg cttgtgccag gcgtccgcgg ttgtcatgac
    21121 ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta gtgaagaatc cctacctgac
    21181 ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag cccgcgcaga acattgcacc
    21241 cagtgcggtg atcgacgcga cggcgaagct ggggaacaat gtatcgattg gcgctaacgc
    21301 ggtgattgaa tccggcgttg aactgggcga taacgtgatt atcggtgccg gttgcttcgt
    21361 aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg gcgaacgtaa ccatttacca
    21421 tgagatccag atcggtcaga attgcctgat ccagtccgga acagtggtag gcgcagacgg
    21481 ctttggttat gccaacgatc gtggtaactg ggttaagatc ccacagattg gtcgcgtaat
    21541 tattggcgat cgcgtggaga tcggtgcctg tactaccatc gatcgcggcg cgctggatga
    21601 cactgttatt ggcaatggcg tgatcattga taaccagtgc cagattgcac ataacgtcgt
    21661 gattggcgac aatacggcgg ttgccggtgg cgtcattatg gcgggcagcc tgaaaattgg
    21721 tcgttactgc atgatcggcg gagccagcgt aatcaacggg catatggaaa tatgcgacaa
    21781 agtgacggtt acgggcatgg gtatggtgat gcgtcccatc actgaaccag gcgtctattc
    21841 ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa accgctgcac tggtgatgaa
    21901 cattgatgac atgagcaagc gtctgaaatc gcttgagcgc aaggttaatc aacaagacta
    21961 acgttccatc ttttgttcgc caaactttac ggcctgtctc attcttacga ttgcggcagg
    22021 ccgtgttatt attgtcgttt cttatatttt gacaggaaga gtatcttgac tactaacact
    22081 catactctgc agattgaaga gattttagaa cttctgccgc accgtttccc gttcttactg
    22141 gtggatcgcg tgctggattt tgaagaaggt cgttttctgc gcgcagtaaa aaatgtctct
    22201 gtcaatgagc cattcttcca gggccatttc cctggaaaac cgattttccc gggtgtgctg
    22261 attctggaag caatggcaca ggcaacaggt attctggcgt ttaaaagcgt aggaaaactg
    22321 gaaccgggtg agctgtacta cttcgctggt attgacgaag cgcgcttcaa gcgcccggtc
    22381 gtgcctggcg atcaaatgat catggaagtc actttcgaaa aaacgcgccg cggcctgacc
    22441 cgttttaaag gggttgctct ggtcgatggt aaagtagttt gcgaagcaac gatgatgtgt
    22501 gctcgtagcc gggaggcctg atacgtgatt gataaatccg cctttgtgca tccaaccgcc
    22561 attgtggaag agggcgcgtc aattggcgcg aacgcacaca ttggtccttt ttgtatcgtt
    22621 ggaccccatg tcggaattgg tgagggtacc gtactgaaat ctcacgttgt cgtgaatggt
    22681 catactaaaa ttggccgcga taatgagatt tatcagttcg cctccatcgg cgaagttaac
    22741 caggatctga aatatgctgg cgaaccgacc cgtgtggaaa tcggcgatcg taaccgcatt
    22801 cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg gtggattgac gaaggtgggc
    22861 agcgacaact tactgatgat caacgcgcac attgcgcacg attgtacggt aggtaaccgc
    22921 tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat cggttgacga cttcgcgatc
    22981 atcggcggca tgaccgcagt ccatcagttc tgcatcattg gtgcgcacgt gatggttggc
    23041 ggctgctccg gtgtggcgca ggacgtccct ccttatgtca ttgcgcaggg taaccacgca
    23101 acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg gattcagccg tgaggcgatt
    23161 accgctatcc gcaatgcgta taagctgatt tatcgtagcg gtaaaacgct cgatgaagtg
    23221 aaaccggaaa ttgctgaact ggcggaaaca tatccggaag tgaaagcctt taccgatttc
    23281 ttttcacgct caacgcgcgg tctgattcgt taatgactga acagcgtcca ttaacgattg
    23341 ccctggtcgc cggagaaacc tccggcgata tcctgggggc cggtttaatc cgcgctctga
    23401 aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg gccacgaatg caggctgaag
    23461 gctgcgaagc ctggtacgaa atggaagaac tggcggtgat gggcattgtt gaagtgctcg
    23521 gtcgtctgcg tcgcttactg catattcgtg ccgatctgac aaagcgtttt ggcgaactga
    23581 agccagatgt ttttgttggt attgatgcgc ctgacttcaa tattactctt gaaggtaacc
    23641 tcaaaaagca gggtatcaaa accattcatt acgtcagtcc gtcagtctgg gcgtggcgac
    23701 agaaacgtgt tttcaaaata ggcagagcca ccgatctggt gctcgcattt ctgcctttcg
    23761 aaaaagcgtt ttatgacaaa tacaacgtac cgtgccgctt tatcggtcat accatggctg
    23821 atgccatgcc attagatcca gataaaaatg ccgcccgtga tgtgctgggg atccctcacg
    23881 atgcccactg cctggcgttg ctaccgggga gccgtggtgc agaagttgaa atgcttagtg
    23941 ccgatttcct gaaaacggcc cagcttttgc gccagacata tccggatctc gaaatcgtgg
    24001 tgccactggt gaatgccaaa cgccgcgagc agtttgaacg catcaaagct gaagtcgcgc
    24061 cagacttttc agttcatttg ctggatggga tgggccgtga ggcgatggtc gccagcgatg
    24121 cggcgctact ggcgtcgggt acggcagccc tggagtgtat gctggcgaaa tgcccgatgg
    24181 tggtgggata tcgcatgaag ccttttacct tctggttggc gaagcggctg gtgaaaactg
    24241 attatgtctc gctgccaaat ctgctggcgg gcagagagtt agtcaaagag ttattgcagg
    24301 aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc gctgttggcg aacgggaaaa
    24361 tcagccacgc gatgcacgat accttccgtg aactgcatca gcagatccgc tgcaatgccg
    24421 atgagcaggc ggcacaagcc gttctggagt tagcacaatg atcgaatttg tttatccgca
    24481 cacgcagctg gttgcgggtg tggatgaagt cggacgcggg ccgttagttg gcgcggtcgt
    24541 caccgctgcg gtgatccttg acccggcgcg cccgattgcc gggctgaatg attccaaaaa
    24601 gctgagcgaa aaacgccgtc tggcgctctg tgaagagatc aaagagaaag cgttgagctg
    24661 gagtctgggc cgcgcggaac cccacgaaat cgacgagctg aacattcttc atgcgaccat
    24721 gctggcgatg cagcgtgccg tcgctgggct gcatattgcg ccggaatatg tgttgattga
    24781 tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct gtggtgaaag gcgatagccg
    24841 cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg acgcgtgacg ccgaaatggc
    24901 ggcgctggat attgttttcc cgcaatatgg ttttgcccaa cacaaagggt acccaaccgc
    24961 ttttcatctg gaaaaactgg ctgaacacgg cgcgaccgaa caccatcggc gcagctttgg
    25021 gcctgtcaaa cgcgcactgg gacttgcgtc ctgattcttg tgtcgagatt aagtaaaccg
    25081 gaatctgaag atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg actactcgat
    25141 gatcgatggc ctggccaaaa ctgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc
    25201 agcactggcg atcaccgatt tcaccaacct ttgtggtctg gtgaagttct acggagcggg
    25261 acatggcgca gggattaagc ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct
    25321 gggtgatgag ttaacccacc tgacggtact ggcggcgaac aataccggct atcagaatct
    25381 gacgttgctg atctcaaaag cgtatcagcg cgggtacggt gccgctgggc cgatcatcga
    25441 tcgcgactgg cttatcgaat taaatgaagg gttgatcctt ctttccggcg ggcgcatggg
    25501 cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt
    25561 ttatgaagaa cacttcccgg atcgctattt tctcgagctg atccgcaccg gcaggccgga
    25621 tgaagaaagc tatctgcacg cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt
    25681 ggcgactaac gatgtgcgct ttatcgacag cagcgacttt gacgcacacg aaatccgcgt
    25741 cgcgatccac gacggcttta ccctcgacga tcctaaacgc ccgcgtaact attcgccgca
    25801 gcaatatatg cgtagcgaag aggagatgtg tgagctgttt gccgacatcc ccgaagccct
    25861 tgccaacacc gttgagatcg ccaaacgctg taacgtaact gtgcgtcttg gtgaatactt
    25921 cctgccgcag ttcccgaccg gggacatgag caccgaagat tatctggtca agcgtgcaaa
    25981 agagggcctg gaagagcgtc tggccttttt attccctgac gaggaagaac gtgttaagcg
    26041 ccgcccggaa tatgacgaac gtctggagac tgaacttcag gttatcaacc agatgggctt
    26101 cccgggctac ttcctcatcg ttatggaatt tatccagtgg tcgaaagata acggcgtacc
    26161 ggtagggcca ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac
    26221 cgacctcgat ccgctggaat ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt
    26281 ctccatgcct gacttcgacg ttgacttctg tatggagaaa cgcgatcagg ttatcgagca
    26341 cgtagcggac atgtacggtc gtgatgcggt atcgcagatc atcaccttcg gtacaatggc
    26401 gtcgaaagcg gtgatccgcg acgtaggccg cgtgctgggg catccgtacg gctttgtcga
    26461 tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc
    26521 cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat
    26581 ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag cacgccggtg gggtggttat
    26641 cgcgccgacc aaaattaccg attttgcgcc gctttactgc gatgaagagg gcaaacatcc
    26701 ggtcacccag tttgataaaa gcgacgttga atacgccgga ctggtgaagt tcgacttcct
    26761 tggtttgcgt acgctcacca tcatcaactg ggcgctggag atgatcaaca agcggcgggc
    26821 gaagaatggc gagccgccgc tggatatcgc cgcgatcccg ctggatgaca agaaaagctt
    26881 cgacatgctg caacgctcgg aaaccacggc ggtattccag cttgaatcgc gcggcatgaa
    26941 ggacctgatc aagcgtctgc aacctgactg cttcgaagat atgatcgcac tggtggcgct
    27001 gttccgccca ggtccgttgc aatcagggat ggtggataac tttatcgacc gtaaacatgg
    27061 tcgcgaagaa atctcctatc cggacgtaca gtggcagcat gaaagcctga aaccggtact
    27121 ggagccaacc tacggcatca tcctgtatca ggaacaggtc atgcagattg cgcaggtgct
    27181 ttctggttat accctcggtg gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc
    27241 ggaagagatg gctaagcagc gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa
    27301 cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa ttcgcgggtt acggatttaa
    27361 caaatcgcac tctgcggcct atgctttggt gtcatatcaa acgttatggc tgaaagcgca
    27421 ctatccggcg gagtttatgg cggcggtaat gaccgccgat atggacaaca ccgagaaggt
    27481 ggtgggcctg gtggatgagt gctggcggat ggggctgaaa atcctgccac cagatataaa
    27541 ctccggtctt taccatttcc acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc
    27601 catcaaaggg gtaggtgaag gtccgattga ggccatcatc gaagcccgta ataaaggcgg
    27661 ctacttccgc gaactgtttg atctctgcgc ccgtaccgac accaaaaagt taaaccggcg
    27721 ggtgctggaa aaactgatca tgtccggggc gtttgaccgt cttgggccac accgcgcggc
    27781 gctgatgaac tcgctgggtg atgcgttaaa agcggcagat caacacgcga aagcggaagc
    27841 tatcggtcag gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc
    27901 ctacgccagc tgccaaccgt ggccggagca ggtggtgtta gatggggaac gtgaaacgtt
    27961 aggtctgtac cttacgggac accctatcaa ccagtattta aaagagattg agcgttatgt
    28021 cggaggcgta aggctgaaag acatgcaccc gacagaacgt ggtaaagtca tcacggctgc
    28081 ggggctcgtt gttgccgcgc gggttatggt caccaagcgc ggcaatcgta tcggtatctg
    28141 cacgctggat gaccgttccg ggcggctgga agtgatgttg tttactgacg ccctggataa
    28201 ataccagcaa ttgctggaaa aagaccgcat acttatcgtc agcggacagg tcagctttga
    28261 tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg atggatattg acgaagcccg
    28321 agaaaaatat gctagcgggc ttgctatctc gctgacggac aggcaaattg atgaccagct
    28381 tttaaaccga ctccgtcagt ctctggaacc ccaccgctct gggacaattc cagtacatct
    28441 ctactatcag agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtcta
    28501 tccgagcgat cgtttattaa acgatctccg tggcctcatt ggttcggagc aggtggaact
    28561 ggagtttgac taatacagga atactatgag tctgaatttc cttgattttg aacagccgat
    28621 tgcagagctg gaagcgaaaa tcgattctct gactgcggtt agccgtcagg atgagaaact
    28681 ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa aaaagcgtag aactgacacg
    28741 taaaatcttc gccgatctcg gtgcatggca gattgcgcaa ctggcacgcc atccacagcg
    28801 tccttatacc ctggattacg ttcacctggc atttgatgaa tttgacgaac tggctggcga
    28861 ccgcgcgtat gcagacgata aagctatcgt cggtggtatc gcccgtcttg acggtcgtcc
    28921 ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa gaaaaaattc gccgtaactt
    28981 tggtatgcca gcgccagaag gttaccgcaa agcactgcgt ctgatgcaaa tggctgaacg
    29041 ctttaagatg cctatcatca cctttatcga caccccgggg gcttatcctg gcgtgggcgc
    29101 agaagagcgt ggtcagtctg aagccattgc acgcaacctg cgtgaaatgt ctcgcctcgg
    29161 cgtaccggta gtttgtacgg ttatcggtga aggtggttct ggcggtgcgc tggcgattgg
    29221 cgtgggcgat aaagtgaata tgctgcaata cagcacctat tccgttatct cgccggaagg
    29281 ttgtgcgtcc attctgtgga agagcgccga caaagcgccg ctggcggctg aagcgatggg
    29341 tatcatcgct ccgcgtctga aagagctgaa actgatcgac tccatcatcc cggaaccgct
    29401 gggcggcgct caccgtaacc cggaagcgat ggcggcatcg ttgaaagcgc aactgctggc
    29461 ggatctggcc gatctcgatg tgttaagcac tgaagattta aaaaatcgtc gttatcagcg
    29521 cctgatgagc tacggttacg cgtaattcgc aaaagttctg aaaaagggtc acttcggtgg
    29581 ccctttttta tcgccacggt ttgagcaggc tatgattaag gaaggatttt ccaggaggaa
    29641 cacatgaaca tcattgccat tatgggaccg catggcgtct tttataaaga tgagcccatc
    29701 aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga ttatctggcc acaaaacagc
    29761 gttgatttgc tgaaatttat cgaacataac cctcgaattt gcggcgtgat ttttgactgg
    29821 gatgagtaca gtctcgattt atgcagcgat atcaatcaac ttaatgaata tctcccgctt
    29881 tatgccttca tcaacaccca ctcgacgatg gatgtcagcg tgcaggatat gcggatggcg
    29941 ctctggtttt ttgaatatgc gctggggcag gcggaagata tcgccattcg tatgcgtcag
    30001 tacaccaacg aatatcttga taacattacg ccgccgttca cgaaagcctt gtttacctac
    30061 gttaaagagc ggaagtacac cttttgtacg ccggtgcata tgggcggcac cgcatatcaa
    30121 aaaagcccgg ttggctgcct gttttatgat tttttcggcg ggaataccct caaggctgat
    30181 gtctctattt cggtcaccga gcttggttcg ttgctcgacc acaccgggcc acacctggaa
    30241 gcggaagagt acatcgcgcg gacttttggc gcggaacaga gttatatcgt taccaacgga
    30301 acatcgacgt cgaacaaaat tgtgggtatg tacgccgcgc catccggcag tacgctgttg
    30361 atcgaccgca attgtcataa atcgctggcg catctgttga tgatgaacga tgtagtgcca
    30421 gtctggctga aaccgacgcg taatgcgttg gggattcttg gtgggatccc gcaccgtgaa
    30481 tttactcgcg acagcatcga agagaaagtc gctgccacca cgcaggcaca atggccggtt
    30541 catgcggtga ttaccaactc cacctatgat ggcttgctct acaacaccga ctggatcaaa
    30601 cagacgctgg atgtcccgtc gatccacttc gattccgcct gggtgccgta cacccatttt
    30661 catccgatct accaggggaa aagtggtatg agcggcgagc gtgttgcggg aaaagtgatc
    30721 ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat cgcaggcttc gctgatccac
    30781 attaaaggcg agtatgacga agaggcgttt aacgaagcct ttatgatgca taccaccacc
    30841 tcgcccagtt atcccattgt tgcttcggtt gagacggcgg cggcgatgct gcgtggtaat
    30901 ccgggcaaac ggctgattaa ccgttcggtg gagcgggcgc tgcattttcg caaagaggtc
    30961 cagcggttgc gggaagagtc tgacggttgg tttttcgata tctggcaacc gccgcaggtg
    31021 gatgaagccg aatgctggcc cgttgcgcca ggcgaacagt ggcacggctt taacgatgcg
    31081 gatgccgatc atatgtttct cgatccggtt aaagtcacta ttttgacacc ggggatggac
    31141 gaacagggca atatgagcga ggaggggatc ccggcggcgc tggtggcaaa attcctcgac
    31201 gaacgtggga tcgtagtaga gaaaaccggc ccttataacc tgctgtttct ctttagtatt
    31261 ggtatcgata aaaccaaagc aatgggatta ttgcgtgggt tgacagaatt taaacgctct
    31321 tacgatctca acctgcggat caaaaatatg ctacccgatc tctatgctga agatcccgat
    31381 ttctaccgca atatgcgtat tcaggatctg gcgcaaggga tccataagct gattcgtaaa
    31441 cacgatcttc ccggtttgat gttgtgggca ttcgatactt tgccggaaat gatcatgacg
    31501 ccacatcagg catggcagag acagattaaa ggtgaagtag aaaccattgc gctggaacaa
    31561 ctggtcggta gagtatcggc aaatatgatc ctgccttatc caccgggcgt accgctgttg
    31621 atgccaggag aaatgctgac caaagagagc cgcacggtac tcgattttct actgatgctt
    31681 tgttccgtag ggcaacatta ccccggtttt gaagcggata ttcacggcgc gaaacaggac
    31741 gaagacggcg tttaccgcgt acgagtccta aaaatggcgg gataacttgc cagagcggct
    31801 tacgggcgag taacgtgctg ttaacaaata aaggagacgt tatgctgggt ttaaaacagg
    31861 ttcaccatat tgcgattatt gcgacggatt atgcggtgag caaagctttc tactgcgata
    31921 ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc gcgcgactcg tggaaagggg
    31981 atttggcgct taatgggcaa tatgtgattg agcttttctc ttttccgttc ccgccggagc
    32041 gccccagccg accggaagct tgcggcctgc gtcatctggc ttttagcgtt gatgacatcg
    32101 atgcggcagt ggcgcacctt gaaagccata acgtgaagtg tgaagccatc cgtgtcgatc
    32161 catacacgca aaaacgcttc accttcttta acgatccgga cgggctgccg ttggaactgt
    32221 atgagcagta aggcttgtca tcgccgcatt tgcccggtaa cgtgccgggc attgctactg
    32281 taaaatcgca ccatcatgac actcacgctc aatagacaac ttctcacctc acgccagatt
    32341 ttggtcgcct ttagcggcgg gcttgactcc accgttctgc tgcatcagtt ggtgcagtgg
    32401 cggacggaaa atccgggcgt cactctgcgc gctattcatg tgcatcacgg tttaagtgcc
    32461 aatgccgatg cctgggttac gcattgcgaa aacgtctgcc aacagtggca ggtgccgctg
    32521 gtggtcgaac gcgtacaact tgcgcaagag gggctgggga ttgaggccca ggcgcggcag
    32581 gcgcgttatc aggcatttgc ccgtacccta ttgcccggcg aagtgctggt caccgcgcaa
    32641 catctcgacg atcaatgtga aacctttctg ctggcgctaa aacgcggtag cggccctgcc
    32701 gggctttcgg ctatggcgga agtttcggag tttgccggaa cacggcttat ccgcccgttg
    32761 cttgcccgca cgcgggggga actggcgcag tgggcattgg cgcatggttt acgctggatt
    32821 gaagacgaaa gtaatcagga cgactcatac gatcgtaact ttctgcgcct gcgcgtagtg
    32881 ccgttattgc agcagcgttg gccgcatttt gcggaagcaa cggcccgcag cgccgcactt
    32941 tgtgctgaac aagagagtct gttggatgaa ctgctggcgg atgatttagc acactgccaa
    33001 acgtcgcagg ggacgctgca gattgcgcca atgctggcga tgagtgatgc ccgccgcgcg
    33061 gcgattatcc gtcgctggct ggcagggcag aatgcaccaa tgccttcccg cgacgcgctg
    33121 gtgcggatct ggcaggaagt ggcgctggcg cgggaagatg cctccccctg tttacgcttg
    33181 ggcgcgtttg aaatccgccg ctatcagtcg caactgtggt ggattaaatc cgtcaccggg
    33241 caaagtgaaa ccattatgcc gtggcagacg tggctacaac cgctggaatt accggcgggg
    33301 ctgggaagcg tacagcttac tgcgggaggc gatattcgcc ctccgcgtgc agacgaagcg
    33361 gtcagcgtgc gtttcaaatc ggcaggattg ctgcatattg tcgggcgcaa cggcggacgt
    33421 aagctgaaga aaatctggca agagttaggc gtgccgccgt ggctacgtga caccacgcca
    33481 ctgctgtttt atggcgaaac gctgattgcg gcggcagggg tatttgtgac gcaagaaggt
    33541 gtggctgaag gtgagaatgg cgtaagtttt gtctggcaga aaacgcttag ttaagtgaaa
    33601 gccggataag acgcatcaaa cgtcgcatcc ggcgaaagtc aatcaggact cgctcaccac
    33661 caccgtaccg atttccgggt ggctaaagct ggtaatttta tccagacgta gctcacgggt
    33721 ttcgccagcg gcctcgacga ccagatactc cacatttttg cgggagacta aatcgctggc
    33781 ttttgcctgc aatttttcgc catctttcag ctcaagtgtc agcattaaat gatgctggca
    33841 ggcgagctca agattatcgt aatcatcaca attgattggt tgatacgtat cattcattga
    33901 cataatcgct caccagtaag tttgccgcag cgtatgctgc tttttccctg acagcctcag
    33961 aaagggcgtc gtcggcagcc atttcattca gcactttcaa aacgcagccc agcgcgtccg
    34021 gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg cttgcgtatt aactcacaat
    34081 atttttccac atgccctcct gtcagcacac tgacttaacc gtggatgcaa gtctaagcct
    34141 acgaagataa actctgtttc gcaaggtgac tataccacac tcatttctgc aatatcagcg
    34201 ccgcaactgc acgtattccg ttacaatggc ctcctgattc gaaaggagtt ttcttatggc
    34261 gcttaaagcg acaatttata aagcgacggt taatgtggcc gatctcgacc gcaaccagtt
    34321 tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa acccaggagc gtatgatgct
    34381 gcgcctgctg gcgtggctga aatatgctga tgaacgtctg caatttaccc gtggtttgtg
    34441 tgccgatgat gagccggaag cgtggctgcg taacgatcac ctgggcattg atttgtggat
    34501 tgagctgggg ctgccggatg agcggcggat taagaaagcc tgcacccagg ccgcagaagt
    34561 ggcgctgttt acctataata gtcgggcggt gcaaatctgg tggcagcaaa atcagagcaa
    34621 atgtgtgcag tttgccaatc tttccgtctg gtatctggac gatgaacaac tggcgaaagt
    34681 aagcgccttt gccgatcgta ccatgacgct gcaggcaacg attcaggatg gcgtgatctg
    34741 gttatcggac gataagaata atctggaagt taacttaacc gcctggcaac aaccttcatg
    34801 attgtgattt cccgacatgt tgctattccc gatggtgagc ttgagatcac cgccattcgt
    34861 gcgcagggcg cgggcgggca gcatgttaat aagacctcaa cggccattca tctgcgtttt
    34921 gacattcggg cgtccagcct gccagagtat tacaaagagc gtctgctcgc cgccagccat
    34981 catttgatca gcagtgatgg cgtgattgtc attaaggcac aggaataccg cagtcaggaa
    35041 ctgaaccgcg aagccgcgct agcccggctg gtggcagtga ttaaagattt aacaacagaa
    35101 caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag gctggcatag
    35161 aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg tcgggaataa
    35221 aaagaaggaa tagatggtga aaaaagcgat agtgacagcg atggctgtaa tcagcctctt
    35281 tactctgatg ggatgtaata accgggccga agtcgatacg ctttctccag cacaggctgc
    35341 cgaactgaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgctg attgtgaagg
    35401 aatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg agcgttatct
    35461 cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc gaaccgctga
    35521 caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga aaggcgatgc
    35581 gctggagatg ctcgatcgtg aaggcaatcc gattgaatcg cagttcaact atacgctgga
    35641 accggcacaa tccagtttgc ctatgacgcc gatgaccctg cgaggcatgt atttttatat
    35701 ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg tagcgaataa
    35761 cgcagagctg gagcgtggct acctggctgc gcgcggtcac agtgaaaaac cggtgttact
    35821 gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc caaccaaagt
    35881 attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta gtttggggca
    35941 gtaaacctgc ttaaggcaga aacaaacgca aaacaggcca gaaggatata tttcaacatt
    36001 ttgaatttgc acgttttttg taggccggat aaggcgttta cgccgcatcc ggcaatggtg
    36061 ctcaacgcct gatgcgacgc tggcgcgtct tatcatgcct acaacccccc tcatacctta
    36121 aggcggataa ggcatttact ttatcaccgg gtcaaaccaa caaagcgacc cgccttgata
    36181 taaatccctg gcttcaggtg cccaacatac tgtaactgcg tttctggctt aaacgcagag
    36241 acatcaccaa tacgcatatg catcagatcg gcagggctaa tccagcccga ttgcgccagt
    36301 gtaagtggat gtccggcttt ggcaaatgca tcagtgacaa attccgaaca aaaccacgac
    36361 tttttgtctc cttcgcccac actgcttaac tgcgctttcg ccaggccgct gacgcactgt
    36421 tggcgaaaat cctcggagaa cggattcagt gagcacatct ggcgagtcac cataaaggga
    36481 ataaattcga caatgccgcg atagttataa ccgctatctt tgattttatt ggcaaacgcg
    36541 gtgatatctg tggcttgttg cggggtaaga tccgggactc gtaagacgaa aagcttatca
    36601 ctatgcttca tcgctttttt aagggaaaca atatggacgc cagcgcctgt cgcttctgca
    36661 acgttattat cacccagaaa gattgcaatg tgactcacag aggaagtgct gaagacgcgg
    36721 attccaaatg aggttacccc aaggcttgag gagaacagca aatcgccggg tttgagatca
    36781 ggtgccgtta tttcttttat tgattgttcg gtgaaagagc tttgatgctg gaatttgaca
    36841 gcccatgttt ttgcctcggc atctactgct gtggctgatg ggtctggctg gctgatatca
    36901 acggtacaag ccgaaagtaa aagaaagctg gggagaagca ggcggcagta cgcctttggt
    36961 ttatccattt tatacaatcc atgtaaaaaa gggccctgaa attcaggccc ctttctggca
    37021 tcagccttta atctgtttca ccagatattc gacgatgtca ccagtcttaa ttaactgttt
    37081 ctcgccgttg cgacgatatt tatattcgat atcgtcgttg tcgaggttac ggtcgcccag
    37141 cacaatagtg tgcggaatac cgatcagttc catatcagca aacatcacgc ccggacgctc
    37201 tttgcggtca tccagcagca cttcgatacc ttgtgcacgc agttcgctgt acagtttctc
    37261 agcaagctct tgtacgcgga aggatttgtg catgttcatc ggcagaatcg ccacctggaa
    37321 cggcgcgata gcgtcaggcc atacgatgcc gcgttcgtcg tagttctgct caatcgccgc
    37381 agctaccaca cgcgttaccc cgataccgta gcaacccatc gtcaggattt ggttacggcc
    37441 atcttcaccc tgtacggagg ctttcagtgc ttcggagtac ttggtaccca gctggaagat
    37501 gtgaccaact tcgataccac gtttgatcag cagcgtaccc tggccatccg ggcttggatc
    37561 gccagccacc acgttacgga tatctgcaac ttccggggta gcgacatcgc gatcccagtt
    37621 gataccgaag tagtgtttac catcgatgtt agcaccagca gcgaaatcac tcatcgccgc
    37681 aacggtacgg tcaatcacca ccggaatcgg catgtttacc ggacccagtg aacccggacc
    37741 ggctttaacc acagcacgaa tttcttcttc ggtcgcgaaa gtcagcgggc ttgcaacctg
    37801 cggcagtttt tctgctttaa cttcgttcag ctcgtgatca ccgcgcacca gcagcgcaac
    37861 cagcgggaag ctgctgcctt caaccgcttt aaccagcaga gtcttaaccg ttttctcaat
    37921 cggcagattg aactgttcaa ccagttccgc gatggttttc gcgttcggcg tatcaaccag
    37981 cgtcatttcc tgggtagcag cagcgcgcgg ttctttcggc gcgatagctt ctgccagttc
    38041 aatgttcgct gcatagtcag aggtgtcgga gaagaccaca tcgtcttcac cgctctgcgc
    38101 cagcacctgg aattcgtgag aggcgctgcc gccgatagaa ccggtgtcgg cttgcacggc
    38161 gcggaaatcc agccccatgc ggctgaagat tttgctgtag gccgcataca ttgcatcgta
    38221 ggtttcctgc agggattcct gagaagtatg gaaagagtaa gcatctttca tcaggaattc
    38281 gcgggaacgc atgacgccga aacgcggacg cacttcgtcg cggaacttgg tctggatctg
    38341 atagaagttc agcggcagct gtttgtaaga gctaagctcg ttacgaatca ggtcagtgat
    38401 aacttcttca tgagttgggc cgagtacgaa cggacgctcg ccacggtcaa caaaacgcag
    38461 cagttccgga ccgtactgtt cccaacgacc actctcttgc cacaaatcgg ctggctgaac
    38521 caccggcatc gacacctcga ttgcaccggc gttgttcatc tcttcacgca cgatgttttc
    38581 gacttttttc agaacgcgca cgccggtcgg cagccaggta tataacccgg aggccagctt
    38641 gcggatcatc ccggcgcgca gcatcagctg atggctgatc acctcggcgt cggcaggtgt
    38701 ctccttgaga gtggagagca ggtattggct agtacgcatg ttgttacggt tccagttgga
    38761 aggtagaaca ggctcaaggc gagcctggga caaaaaaagt gatttagttt accagtgcaa
    38821 aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa gacttcaaaa
    38881 cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc ataggttttg
    38941 cccgtttcct cacctttatg ataggccggg cgcgggtcct gcgccagtac ttcgcggata
    39001 aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc gacctctgcg
    39061 gtaaaactca ccgccatctc tgcagctggc gcactttgcg catagctggc actggcatcg
    39121 ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt accatcgacc
    39181 agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac ctctttcagc
    39241 tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc gaaaaccccc
    39301 attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc gccttccatc
    39361 gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc gcgaacggcg
    39421 tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt taccagacct
    39481 ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc tatttgctcg
    39541 aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat acagcctgac
    39601 gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc gcattggctt
    39661 tcattttaga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc ggcggagagt
    39721 cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg aacggtttgc
    39781 cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc gctttcggtt
    39841 ttgcaggctc cgctttcggc tggggtgcag tgctttgaac gggttcgaca ggggatctgc
    39901 ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa gcacgcatag
    39961 tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta aaaatgacaa
    40021 gacgggcaag tgcccatcct gaatgatatt acaaattgtg gaaacagcct aaaaattacc
    40081 agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact tcatcagact
    40141 gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca cgcgttacga
    40201 tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc gctggagtca
    40261 ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgc gcgtcgtcca
    40321 gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt gggttctcaa
    40381 caacatcaag tacggtcggc agcaggccaa cgccatcttt cagtttgatc aagcccactt
    40441 tttgcagcag cagcagtgaa cgaccaaggt tagttggatc gtttggcacg gcaacctgcg
    40501 aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca atcggataaa
    40561 caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc tgatcaaggt
    40621 acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct tcgtttggca
    40681 gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct ttcgcaactt
    40741 tctgcgcgac ttctgcaacc tgctgttcgg caccaacaat cacgccgact ttaatgtggt
    40801 ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc agggctccca
    40861 ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat tatttatgtt
    40921 gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg aactgaatta
    40981 aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg ttatagccga
    41041 tgtagccata ctgatagcca atctgaccta aaccaccggc accgacagca ccacccatcg
    41101 cggaataacc gaccagggta atcagggtga tagttgccgc attcaccaga cccggcaatg
    41161 cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt gcacgggaag
    41221 cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg gcaataaacg
    41281 gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat gtaccaacaa
    41341 taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggata gaacggaaaa
    41401 tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt tgccccggac
    41461 gcgtgacata aagcagaacg ccaaccggca gaccaatcac aaagccaaaa aaaccggata
    41521 cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag cagccacatc atcggctcag
    41581 acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat ggcggcttgc
    41641 gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac gccaccggcg
    41701 tagtccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc ggtttcagaa
    41761 agcagtgggg catcgaccga ttgaccggta aactccagac gcagcatcgg cacgcagtca
    41821 gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag atgcagggtc
    41881 gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc acttaccgtg
    41941 tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat acgcttcaca
    42001 acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt aatgtctttc
    42061 agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc ttcatcacac
    42121 agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg tttctgccca
    42181 ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa tgacagcaat
    42241 tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag cggcagagcc
    42301 acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat cataccaatc
    42361 tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg gccatcgacc
    42421 agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag cgtactctta
    42481 cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg caggctgacg
    42541 ttgttcaacg cctggatggt gcgggtgccc tggtggaaca ctttggtgat attcgaaagt
    42601 tttatcattg attatttatt atcgtcatta agttagtcgt ggcatctcga atgcctgaaa
    42661 cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat caatcaggtt
    42721 tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtcac atgccttatt
    42781 aaggagctat aaaaggtggc aaagagcgta cccgcaattt ttcttgaccg tgatggcacc
    42841 attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttac cgacggtgtt
    42901 attgacgcca tgcgcgagct aaaaaaaatg ggctttgtgc tggtggtagt aaccaaccag
    42961 tctggcattg ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac cgagtggatg
    43021 gactggtcgc tggcggaccg agatgtcgat ctggatggta tctattattg cccgcatcat
    43081 ccgcagggta gtgttgaaga gtttcgccag gtctgcgact gccgtaaacc acatccgggg
    43141 atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta tatggtgggc
    43201 gataaattag aagatatgca ggcagcggct gcggcgaacg tgggaacaaa agtgctggtg
    43261 cgtacgggta aacctattac gcctgaagca gaaaacgcgg cggattgggt gttaaatagc
    43321 ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcataatg attaaaagat
    43381 gagcgattga aataaaaatg catttttcca cttgtcttcc tgagacgact ccctataatg
    43441 cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt cggttgaaga
    43501 gaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata cgccacctcg
    43561 cgacagtgcg ctaaagcgcg ttgcaactgc tctttaacaa tttatcagac aatctgtgtg
    43621 ggcgctcgaa gatacggatt cttaacgtcg caagacgaaa aatgaatacc aagtctcaag
    43681 agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt taaattgaag
    43741 agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca agtcgaacgg
    43801 taacaggaaa cagcttgctg tttcgctgac gagtggcgga cgggtgagta atgtctggga
    43861 aactgcctga tggaggggga taactactgg aaacggtagc taataccgca taacgtcgca
    43921 agaccaaaga gggggacctt cgggcctctt gccatcggat gtgcccagat gggattagct
    43981 agtaggtggg gtaacggctc acctaggcga cgatccctag ctggtctgag aggatgacca
    44041 gccacactgg aactgagaca cggtccagac tcctacggga ggcagcagtg gggaatattg
    44101 cacaatgggc gcaagcctga tgcagccatg ccgcgtgtat gaagaaggcc ttcgggttgt
    44161 aaagtacttt cagcggggag gaagggagta aagttaatac ctttgctcat tgacgttacc
//
LOCUS       DAJTMK010000010        42574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTMK010000010 DAJTMK010000000
VERSION     DAJTMK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42574
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.010"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..753)
                     /locus_tag="OO135_000483"
     CDS             complement(<1..753)
                     /locus_tag="OO135_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5396444.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQEFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSTLTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFN"
     gene            complement(753..2336)
                     /locus_tag="OO135_000484"
                     /pseudo
     CDS             complement(753..2336)
                     /locus_tag="OO135_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(2368..3192)
                     /locus_tag="OO135_000485"
     CDS             complement(2368..3192)
                     /locus_tag="OO135_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5396445.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
     gene            complement(3452..4705)
                     /gene="amiC"
                     /locus_tag="OO135_000486"
     CDS             complement(3452..4705)
                     /gene="amiC"
                     /locus_tag="OO135_000486"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5396446.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            4937..6268
                     /gene="argA"
                     /locus_tag="OO135_000487"
     CDS             4937..6268
                     /gene="argA"
                     /locus_tag="OO135_000487"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5396447.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(6330..8156)
                     /gene="recD"
                     /locus_tag="OO135_000488"
     CDS             complement(6330..8156)
                     /gene="recD"
                     /locus_tag="OO135_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5396448.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(8156..11698)
                     /gene="recB"
                     /locus_tag="OO135_000489"
     CDS             complement(8156..11698)
                     /gene="recB"
                     /locus_tag="OO135_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5396449.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVTSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVVTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(11691..14579)
                     /gene="ptrA"
                     /locus_tag="OO135_000490"
     CDS             complement(11691..14579)
                     /gene="ptrA"
                     /locus_tag="OO135_000490"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5396450.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(14755..18123)
                     /gene="recC"
                     /locus_tag="OO135_000491"
     CDS             complement(14755..18123)
                     /gene="recC"
                     /locus_tag="OO135_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5396451.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(18136..18459)
                     /locus_tag="OO135_000492"
     CDS             complement(18136..18459)
                     /locus_tag="OO135_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5396452.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(18444..18851)
                     /locus_tag="OO135_000493"
     CDS             complement(18444..18851)
                     /locus_tag="OO135_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5396453.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQLAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(18848..19411)
                     /locus_tag="OO135_000494"
     CDS             complement(18848..19411)
                     /locus_tag="OO135_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5396454.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(19402..19872)
                     /locus_tag="OO135_000495"
     CDS             complement(19402..19872)
                     /locus_tag="OO135_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5396455.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20057..20851)
                     /gene="thyA"
                     /locus_tag="OO135_000496"
     CDS             complement(20057..20851)
                     /gene="thyA"
                     /locus_tag="OO135_000496"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5396456.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(20858..21733)
                     /gene="lgt"
                     /locus_tag="OO135_000497"
     CDS             complement(20858..21733)
                     /gene="lgt"
                     /locus_tag="OO135_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5396457.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(21884..24130)
                     /gene="ptsP"
                     /locus_tag="OO135_000498"
     CDS             complement(21884..24130)
                     /gene="ptsP"
                     /locus_tag="OO135_000498"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5396458.1"
                     /translation="MLTRLREIVEKVASAPCLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLWRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(24143..24673)
                     /gene="rppH"
                     /locus_tag="OO135_000499"
     CDS             complement(24143..24673)
                     /gene="rppH"
                     /locus_tag="OO135_000499"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5396459.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGITPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            25358..26047
                     /gene="mutH"
                     /locus_tag="OO135_000500"
     CDS             25358..26047
                     /gene="mutH"
                     /locus_tag="OO135_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5396460.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLQEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            26116..26829
                     /locus_tag="OO135_000501"
     CDS             26116..26829
                     /locus_tag="OO135_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5396461.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            26967..27185
                     /gene="ygdR"
                     /locus_tag="OO135_000502"
     CDS             26967..27185
                     /gene="ygdR"
                     /locus_tag="OO135_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5396462.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            27293..28333
                     /locus_tag="OO135_000503"
     CDS             27293..28333
                     /locus_tag="OO135_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5396463.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(28365..29558)
                     /gene="lplT"
                     /locus_tag="OO135_000504"
     CDS             complement(28365..29558)
                     /gene="lplT"
                     /locus_tag="OO135_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5396464.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLIALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(29551..31710)
                     /gene="aas"
                     /locus_tag="OO135_000505"
     CDS             complement(29551..31710)
                     /gene="aas"
                     /locus_tag="OO135_000505"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5396465.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            32099..33130
                     /gene="galR"
                     /locus_tag="OO135_000506"
     CDS             32099..33130
                     /gene="galR"
                     /locus_tag="OO135_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5396466.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(33137..34399)
                     /gene="lysA"
                     /locus_tag="OO135_000507"
     CDS             complement(33137..34399)
                     /gene="lysA"
                     /locus_tag="OO135_000507"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5396467.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGPVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            34521..35456
                     /gene="lysR"
                     /locus_tag="OO135_000508"
     CDS             34521..35456
                     /gene="lysR"
                     /locus_tag="OO135_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5396468.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(35443..36135)
                     /gene="ygeA"
                     /locus_tag="OO135_000509"
     CDS             complement(35443..36135)
                     /gene="ygeA"
                     /locus_tag="OO135_000509"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5396469.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(36264..37682)
                     /gene="araE"
                     /locus_tag="OO135_000510"
     CDS             complement(36264..37682)
                     /gene="araE"
                     /locus_tag="OO135_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5396470.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSHLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMITTLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGI"
     gene            complement(37997..38758)
                     /gene="kduD"
                     /locus_tag="OO135_000511"
     CDS             complement(37997..38758)
                     /gene="kduD"
                     /locus_tag="OO135_000511"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5396471.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(38788..39624)
                     /gene="kduI"
                     /locus_tag="OO135_000512"
     CDS             complement(38788..39624)
                     /gene="kduI"
                     /locus_tag="OO135_000512"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5396472.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVRYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(39911..41092)
                     /locus_tag="OO135_000513"
     CDS             complement(39911..41092)
                     /locus_tag="OO135_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5396473.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            41347..>42574
                     /locus_tag="OO135_000514"
     CDS             41347..>42574
                     /locus_tag="OO135_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5396474.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNPLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        10771 a        11383 c        10134 g        10286 t
ORIGIN      
        1 attaaaatta tcgtcagttg aaatcatata tgatccggtt agcgcaggaa cattaaatgt
       61 tttcgaaatc gaattaaaca ccaccacttt gtcatctttt attgttagcg ttgacgtgaa
      121 actatgatta aaaacaaaat cactatgtat ttcatctgaa cataaccaaa tattatgcct
      181 tgcacagcat gctgcgattc tgtgaagatc gtcttccgac cagaccgttc ctgtcgggtt
      241 atgaggatta cataataaca ccatcttcgc gcttttaatt ttaagctcaa actcttgcca
      301 gttgataacg tagcctctcc cattttcttt catcaaagag gttgttagca tgtttcgacc
      361 attatgctta atcacatcaa aaaatgcgtt ataaacaggc gtgaaaatga gaatattatc
      421 tccattcaat gtcaatattt ctattgcctt agatattgaa tacatcacgc ttggactata
      481 ggtgatccat tctggttgaa tctctgtttt atattgttga tgataccaat tgactatcga
      541 attcagatag tcatcatgct gccaacgact gtaccctaaa atcgggtgtt ccagacgttt
      601 ttttaatgtt ctgatgatag cttccggtat cggaagatcc atatctgaaa tagagaatgg
      661 taatacatct tttacaccaa atctgtcttc acaaaagtcc cattgtgtgc aatatgttcc
      721 tttacgagaa attgaggtat taaaaatatc catcaacata tccccatgta attatccata
      781 tcccgacgta aggcgatgac ttttgtaccg ataatgactt gtaaggtatt ttgatcaagt
      841 ttcacaaccg caattcctcc ggcccttttt attgcagcct catctatcaa tcccatatcc
      901 gcgatagtta aacgcaaacg agttacacaa ttatccagtg atttaatatt ttctttacca
      961 cccagagcct taaccatttg ctccgctgcc tgcaactctg atttatcatg aactgggatg
     1021 ttagctattg cagtttcttc gtgagcgttc agcgtgacag gactggactc ttcacggcct
     1081 acagtcatca ggttaaattt gagaatggtc caacggaaca caaagtaata aatgaagaaa
     1141 taagccactt caacacatac cgctaatggc catcccgttc ttgtgccacg taagacacca
     1201 aagctaataa attgaatcag atcgccagta aagcccatct tcacgcccat atatggcaga
     1261 accatgttag ccaatccatt cataaaggca tggaagacaa acaacagcgg gctgacaaat
     1321 aagaacagaa attcaatcgg ctcgctgacg ccgccaataa ttaccgtgag cacaccggaa
     1381 atcagcaatg ctttgatagc ccttctgttt tgagcatgag cagtgcgata catcgccagc
     1441 gcggctccag ccagaccata ctgaatcatc agtttgccct gctccatttt ccccgccaaa
     1501 tccagcggaa tgaccgcatt ctctttgacg tatgccataa acatgttcag ggtgccgtaa
     1561 tactcttcac cgccaatcac tgccgatccg ccgattgggg taaagcggaa aactgatgtc
     1621 accaagtgat ttaagccaaa aggtatcgtc acgcgttcgg caacagcata gaagaaataa
     1681 ccaacaggac cggaagttga aatccagtga ccaatgagca tgaaaagatt gaaaaatggc
     1741 ggccagataa aaggaatgat cagaccaaat ccactcatca caatcagtgt aatgataggc
     1801 acaaggcgtg ggccactata aaaccctaat gattcaggaa tacgtaagtt aacgatcttt
     1861 ttgctggcga ctaataaccc tgccacaatc ccacccaaca cgctggtatt gtaggactgg
     1921 atcccgagta tgatggtttg cccatgtgtc gacatttggt cagcagctac caataagtcg
     1981 tgctgtttaa gataaaagtt cgttccaaga tgcatcgcca taaagccaat taagccaatt
     2041 aagccagaaa aagcgccata ggctttatca tctttctctt ttaataatcc caaaggtata
     2101 gctatcgcaa acaatacagg taaattaacg aaggcaaaca aaccaaggct aacaataaaa
     2161 tcaagtatgg ttttaattaa cggaatagcc aacactggaa tgaattttgc catatcatcg
     2221 ctggctaaac cacttcccag ccctaacatc ataccacata cacttaacag agcgatggga
     2281 tacataaatg ccttccccag gctctgaaaa aaactctagg atttcttttg tttcatactg
     2341 gttatctcat gaaaatgtta tgtataatta gtccattaat actttgatgc gaatagaaag
     2401 atatagtttc tcttccagaa gagaatcata tttaaaagtt gcgtacagat atctttcaat
     2461 ttcatgaatg gcacgttgta actgaaaatc tttttcaaga aactgctcat agatatcacc
     2521 aatatttttc tgttttattt tttcattaca taacacacga ctagaaaaat atttaaggtg
     2581 catcataaat ctggagtagt taaatgaact tctgtccacc cttctgttat aaacaacttc
     2641 aataagctcc gtgatttttt gagacagctc aataatcctt actgattcat aatcaactga
     2701 attttcataa ttatttaaaa tatgcaatgc tataaaacca atctcatcat ccggtagttg
     2761 aacgctaaat tgctcattaa tgattgttaa tgcatcacga cttattgcat actctgcttt
     2821 gtacaacgct ttgatttcct caaggaaaac attattaatt tgtatgcctg agacgactcg
     2881 ggatattgca ttcgaaatgt gatcagcaag aatcagtagt agcgatggat tgagtttttt
     2941 ttcgagatta ctctccgcat aatgcactat cttttcggca acaaaaatat actcaatacc
     3001 cacatgctca atcaatttgt agagtttatt tttttgctca tttctgacat aaaaaatccg
     3061 gtttgcagga tgctcaggga ccgacatacc atatttttta ttgtaaccaa cgccaggccc
     3121 agaaatgata acctcctgtc cgttcaccga tgcctgtaca caattattgt tcatgacttt
     3181 ttcgatgatc attccatggc tcccaaaaaa aaaggcaagg catgtcagaa ataataatta
     3241 cttcaaacat tgccttgcct gatttaacag ttacaagata attaactaat gcgtatacct
     3301 tacacggaat tgtgcttaat aaaagagatg acagacttat tttttaagcc gatattcaaa
     3361 tattcgacag cagtcacaaa accatcgttc aacatttatg taaataatat ttattcaatg
     3421 aaacataaat tcattgaata aattaaaaac atcatcccct tctcgccagc gtcgccccat
     3481 cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc tggaaagtcg
     3541 ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg accagaatgg
     3601 agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga tttttatgca
     3661 gtttgttgat tttaccgagc ttattcagta ccgctttacc aaacttcagg ctgtcggcaa
     3721 tggtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc
     3781 tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt gccgcagtac
     3841 tggttgcacc tttggttgag agcgcaaaca cagaggaacc gctcggctga cgactggtaa
     3901 aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct tttgctacgc
     3961 gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc atattgccct
     4021 ctttctcgat cagagagcgc agacggcgag ctatttgcaa taccacgtct ttttcgcgcg
     4081 ttttgtattt ccccaccgca ccggagtctt cgccgccgtg gccagggtca agcataataa
     4141 caataggacg atcgcgccct gctttacccg gttgtggacc actttgtgct ggcggcacct
     4201 gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat
     4261 cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg gcgaccggcg
     4321 caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatc cgtacggtct
     4381 gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg
     4441 ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc accacgcgtt
     4501 cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct accgtcacac
     4561 gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctgacc gcagccagac
     4621 tgacctgact tacgctcaat agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc
     4681 tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa
     4741 caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct gtcatctaat
     4801 aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg ctctcgcata
     4861 aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acactaattt agaataataa
     4921 tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg gattccgcca
     4981 ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga
     5041 agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt tgcacagcct
     5101 cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa atctggccgc
     5161 gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca aaacactgga
     5221 actggtgaag caggctgcgg gaacattgca actggacatc actgctcgcc tgtcgatgag
     5281 tctcaataac acgccgctac agggcgcgca tatcaacgtc gtcagtggca attttattat
     5341 tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc gtatccggcg
     5401 gattgatgaa gacgcgatcc atcgtcaact ggacagcggt gcaatagtgc taatggggcc
     5461 ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga ttgccactca
     5521 actggccatc aaactgaaag ctgagaagat gattggtttt tgctcttcac agggcgtcac
     5581 taatgacgac ggtgatattg tctcagaact tttccctaac gaagcgcaag cgcgggtaga
     5641 agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc gtggcgcagt
     5701 gaaagcctgc cgcagcggcg tgcgtcgctg tcatttaatc agttatcagg aagatggcgc
     5761 gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga tggaaagcgc
     5821 cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt tgattcgccc
     5881 gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga tggaaatcga
     5941 caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc tctatccgtt
     6001 cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt accgcagttc
     6061 atcacggggc gaggttctgc tggaacgaat tgccgctcag gcgaagcaga gcggcttaag
     6121 caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg gatttacccc
     6181 ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc agcgtaaatc
     6241 caaagtgttg atggcggatt tagggtaagc gatgaaaatt gtcggatgcg acatgcgtaa
     6301 cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa cgccgccaga
     6361 ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc atcggcatac
     6421 agcgacagac ggcgacgcgc gcgggtcacc gcggtgtata ccagctctcg cgttactacc
     6481 ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc ctgcgattta
     6541 tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg cacagactta
     6601 atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata
     6661 ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat caccggtcga
     6721 ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg cataaactgc
     6781 tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc gcacaaaagt
     6841 tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg cagcagatcc
     6901 agataacgtc cataacccgc aagagcttcc tcaagcatcg caatataatc ttcgccgctc
     6961 tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt
     7021 ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct gccgaaacga
     7081 tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt gccggttcct
     7141 gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc ggtaaagccc
     7201 gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga ggccagttga
     7261 tcacgatcgc cgagaaagat cactcgcgca tgatcgggca aggcgtcgat cagtctcgac
     7321 atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag atgcagcggg
     7381 ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa
     7441 gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa
     7501 gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc agccagacgg
     7561 atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt cgccacggtg
     7621 gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt cagcgccact
     7681 gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt gtccagggtt
     7741 tgcgccagta gagcttcatc aacctcaatg gcatgattca cctcattgaa gaagcgcgct
     7801 accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca gaggatcatc
     7861 ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc ttcccaattt
     7921 tgtagctcac cgatttcact gacacaggtc gccaacagcg gatgcgacgc ctcgttattt
     7981 tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact taacagtgcc
     8041 gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa ttgcacatcc
     8101 agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa tttcattacg
     8161 cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac ccggcgttgg
     8221 gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc aggaacagat
     8281 agataacgcc gccaaagtga cgatcatagt cgtaatcagc aatgcgatgg cgcagataac
     8341 gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc tgcatcgctg
     8401 ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg gatttatagt
     8461 cgagcaggta ataacgccct tcgtggcgga atactaggtc gataaagcct tttaacatgc
     8521 cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca aactggcgaa
     8581 ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa aactccatct
     8641 ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccttttca ttgagaggtg
     8701 cctggaggac agccgtgatc cactcggtca ccaccggttc ccactgcgat tcaaagccgc
     8761 cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt
     8821 caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga tgcggtgtta
     8881 acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc cgtggcatca
     8941 gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg acacgccagt
     9001 tatcgccggg caatcgttgt agtgtcctcg cattcagttc tgcagttaaa gcatcattaa
     9061 cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata
     9121 acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt tgcagcaaac
     9181 gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga cggcgcacca
     9241 gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac
     9301 gcagatcttc cgccagacgt tcgacctccg cgaggtcgac gctttttggc gcagcattaa
     9361 gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga
     9421 aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat ttatggatcg
     9481 tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg gaggcattac
     9541 tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca ctttccagct
     9601 gcgttccggc ttcttgtagc agttcgctga tatgcaagat atcggtaaga cgccgctcac
     9661 cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca
     9721 tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct tcaactaccg
     9781 catcccacgc atgttcgtca ttattcagcg tttcgatatc cagcgcgttc agccccatca
     9841 ttgacgttgc cagcgcacta cgcagggtgt tctcacgttc gggcgtcatc accgcctgca
     9901 ataaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt
     9961 aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc tcctggcggc
    10021 tgcgcaccag cacactgatg tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca
    10081 gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca gcacatacct
    10141 gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc agccacattt
    10201 tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt ttcccggctg
    10261 atgtcactgg aataaacggt atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa
    10321 gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct aaagtgtagt
    10381 gggcgtgaac ttcactacgc gccttcatat aagtgaagat atccgccccc cggaatgcat
    10441 atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga tggtgccaga
    10501 tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg atcattgcca
    10561 ccgggaatcg cgtacggatc gccgctgcca acacctcacc gctttcgcta cgcagcgcgg
    10621 aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg
    10681 ctactgtttc gcggatctca gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg
    10741 gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt tcccccccgg
    10801 ctttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc ggcaactgat
    10861 aactgttggt ctcttcttct gcccaggcgc tgatcttgtc gatccattta gcctgattgc
    10921 tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc agttcaccca
    10981 ctgcgtcgcg ccactgctgt tttaccgtag caatacgcgc cacaatttgc gcgtggcggg
    11041 aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcgcttcg ccttgcagat
    11101 aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag acgacctggg
    11161 ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag gcctggtaac
    11221 gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat tcaaaggcat
    11281 tcaggttgag catgcgctgg caaaaaccgt gaatagtaaa gactgccgct tcatccatct
    11341 gccgttcggc taacaacaac cactgagcgg cttgcgcttt atcgtcgatc tcttccagca
    11401 gacgttcgta cagtggattg tcggtagttt cacgcagaca ggcgatgcgc aactcatgga
    11461 tattgctacg gatacgacca cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca
    11521 gcagttcttc aacggtcagc gggcggggaa aggcggcgga accgcctagt ccaagtaaca
    11581 ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag gcttcaatca
    11641 ggcgctcacc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca tcactcattc
    11701 ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca cactttccag
    11761 ccttcagggt gtacatattc ggctttcccg ttctggctgc cggaaatctg cgacagaata
    11821 gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt ttgcggcgtc
    11881 agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt gccgcgatcg
    11941 aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg
    12001 gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg caattttgcc
    12061 tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg tttatcattg
    12121 ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa cgcaaacacg
    12181 gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg tacgatctgc
    12241 cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg tacaaatacc
    12301 gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga ttgtttttta
    12361 tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc
    12421 acatcgcgtg cctgcgttgt tgcctgggcc tcggtcatgt tgccgataac cataaactct
    12481 ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa cgtaatggag
    12541 ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag catctgcgcg
    12601 ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat ctggttatac
    12661 caagacttcg cctgctcaag ctgatcttcc gtagcggtat agctaaagta cccctcgagc
    12721 aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaac cataaggccg
    12781 ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct taactgatca
    12841 agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg ggcgctgtcc
    12901 atggctttcg gattacgcaa aatcaggctg acatcagctt tgggttcgct ggaaaaataa
    12961 cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc tggatggtca
    13021 tatttcttct ctgacttaat cagcgagaaa tcgtcaggaa tataggggtt aagctccggt
    13081 aaggagagcg caatattggc agctttttgc tgccagtcag cgaaagtttg ttcgctgatt
    13141 ttatcgacct gatacggcgc atcgacaaaa taagccgttt tattgtgcgg ctctttcggg
    13201 ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc ttttactgct
    13261 ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac cggaacgcga
    13321 atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgatcga cggataacgg
    13381 aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat gcctttttca
    13441 cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt agccaggcct
    13501 ttatcggtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac gataggatcg
    13561 gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcgga aagtgtacct
    13621 gggctgcgat taccaatcag ataggtaatc aattcatccg ttttactacg gaacttcgct
    13681 gagttgttat cgatgcgaaa ctcaacgcgc agaactttac gcggcagtgc cgggacgtaa
    13741 tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc cggttttttg
    13801 ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa ctccggcagc
    13861 ggtttattgc tgtaaataac cgccttcatc agattggcgg aatagtactt ctcgtggaaa
    13921 tctttcagcg cctgctgcac cggattacca ggtttatcgc ttaaagtttc gaggttacca
    13981 ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg
    14041 cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcaccgcatt acgctcacgt
    14101 tcggcatatt tcttgtcgag caaaggttca gcaatagcat cggccaggcg gtctaccgca
    14161 ccaggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg cgcagtgctg
    14221 gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc ctgcgggtac
    14281 tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg gtacgcctcg
    14341 ggatcttcca gcgacccaac gggcaccacc agcgccgaga gcgatttaac tgcctgcgga
    14401 tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata ctggcggtta
    14461 tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc tgcctgactt
    14521 aagggtgccc aaagggcaac taacaacaat aatgctttga accaagtgct gcggggcatt
    14581 cacggacctc ataatcaact taattttctg tccagattca acacgttaac gcaaatcatc
    14641 tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc atcagtccgt
    14701 gactggtgcg catcataaag taagcggata gattgcgcaa tttttataca gcactcatga
    14761 ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga tggcctccat
    14821 tgtctctggt gttaattgcc gccagagcct ttgataccag atatcatcac cttcgccacg
    14881 caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt gcaacgtgga
    14941 atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc acgcgccgcc
    15001 actttcaggt aacaccagca atggcgcgga catcccttca cgatacccct caatcagttg
    15061 tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact cgccgtcttt
    15121 gcgtagaaaa aggcgacttt caccattacc accgctggca cagtagacaa ggtgttccag
    15181 ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca acaggccatc
    15241 cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga gatcaatttc
    15301 catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct gcatctcctg
    15361 gcactgagtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg
    15421 gaagcgacgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca ataactgctg
    15481 attgatttga taacggctaa gcccttccag aataaatggc tcggtgtcgg ggatttcgct
    15541 gtcttcggta cggaagttca cctgcaaacg catctggaaa aatgcccgca ccggatgtgc
    15601 ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg gcagcggctg
    15661 aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt cacgagcata
    15721 gctttgtcgt tcgcctggct ggtagttttg tggatcaaac ggcatccggg tatggaggca
    15781 ggtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg
    15841 tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg acgggaaacg
    15901 ttcactgtta tcctgaatgg aaagcccgat ataactgata tagagttttt gctgcgcgga
    15961 aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt cgccacgctt
    16021 cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc
    16081 gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca tcagagtaca
    16141 aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc
    16201 cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc
    16261 ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc
    16321 cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt ctagcggacg
    16381 ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag ccagatgccc
    16441 caccagttct gcaattaagc cgctcgactc atcataaggt agaaccgatt gccactcgcc
    16501 ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc gccaggtgtg
    16561 ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat
    16621 gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg
    16681 cgccgccagc accggcacat ccagtaatgc cagcacgtct tccgacacaa agcggctgtc
    16741 tggcaacgat aacaggctga taaacgcttc cagtaccgga tgcgactgcc gcgcccgacg
    16801 gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat
    16861 aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg gagtaagtgt
    16921 cgggtcttct tccagcatcg ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg
    16981 gctatggcaa acgtggaagg tgatactgct atccagtgga tcaagcgggc gtttgttatc
    17041 gctacgggaa aactcttcga tgttcacacc agcaacggca cggttttcca gttccagaat
    17101 gtcagactga atgttatgca gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc
    17161 ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct tcccccatga
    17221 ggccagcagc gggttgccga catcctgttc accatcgctg ttaaagagct gtccggcatt
    17281 ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt
    17341 cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac ggcaggggtt
    17401 ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct ggagataaac
    17461 aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt
    17521 cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg
    17581 ttgtccgagt tcatgggtat attccaccag cgccttccac aacggcgctt gccaggcctg
    17641 tgcttctccc agcccttcaa ccaaatgtcc tgtttcccat tgtgccagcc actccgggcg
    17701 atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga acagttttcg
    17761 cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgt gctccaacag
    17821 ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg cgctctcttt
    17881 ggggatctcc ggtaacaccc ggacgaacat atcccagata aagctcgcag gcagcggaaa
    17941 atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc actgtgccat
    18001 accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc
    18061 gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt agacccttaa
    18121 catagcggct cctgactact gacgattcgg gcaatgcagg cgggtcatct cgccttctct
    18181 gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg atgtctgcaa
    18241 tcggttgacc tgccagttag ctggtggcga aatcgcgcgc agttgcgttt gctgccagcc
    18301 atgccgccag agctgttggt actggtttct gctggcaaga ctgttcatta atgttcgctg
    18361 gaaacccgat aacgcagtga caatcatcac catcaacacc atcgccaaca ttacctccgg
    18421 caggctaaag ccttgttgat tcttcaggga agctgacata acgccctctc tttcagcgga
    18481 caaaaatcgc tccagccgct tggcgaaaaa acaatgtttc catcaatgac ttcgcctgtt
    18541 cgccacaacg aaacgccttc ataaccggca atcaataagg cttcattatc tgccagtaaa
    18601 cgcaaacaaa cctgggcatc ggtttcagcg tactgcgagc actgaactgc tagctgcgtc
    18661 tgccaggagt gcatttttcc ccacgccagc gccgactgaa cgatggcctg gcggcgcaat
    18721 gactgacttt ccatgctcac gcgagaggca aaactgcgat cctgttggct cattccttgt
    18781 aatagcaaac tacccaaaac cagcagcatc aggaccagag ccagtgacga aacacccttt
    18841 tcgcggttca caggttgaat cctgtcacgc tatagctggc attcaccacg gtttgcggtt
    18901 cagacttact ggcagcacgc atattaaccg tcaacaccgg cgagaagccg ctgacatcct
    18961 gacgtacgac ctgaaaagtg tcgataatga tggcatccgg attagtgact ttatcccagc
    19021 ccttaccttc acaggatgtc gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa
    19081 atccaatctg gtcagactct tttaccggtt cgcgatccca gataccgtta ctgttcgcat
    19141 cccactgcac aatgatacag tcaccctgtc cgacaatttc cagcccttcg ccggtacaga
    19201 tgccatgaca ataacccgcc ctctggagat gcttcgcgac ggtaaatacc cgcagccaga
    19261 tttcatcttc cagcgccagc ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca
    19321 gaaagcgtgc agcccccagc aacaatacgc tactgatcgc catagcaatc aacacttcca
    19381 gcagagaaaa accttgctct tttacaggca tccttctgtt tctccttgct gacaaagccg
    19441 gagtcttccc cacggcgaaa ccaccagcca ccactcgccc gttgagtttt tgaagcgaat
    19501 atgcccggcc catgcggtat tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct
    19561 catttcgact tcgggccagc gtggcacaaa gaccaatggt gaactgccat gacaggtatt
    19621 ggccccagca gcggaactca caaggcacca taacgtcccc tccctgataa cgctgatact
    19681 gtggtcgcgg ttatgccagt tggcatcttc acgtaaatag agcaaatagt cccgcgcctg
    19741 gctggcggtt tgccatagcc gttgcgactg ctgccagtat tgccagccat agagtccact
    19801 tgcgcttagc atgaccaaaa tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg
    19861 ttgtgttttc atgccggcag tatggagcga ggagaaaaaa agacgagggc cagtttctat
    19921 ttcttcggcg catcttccgg actatttacg ccgttgcagg acgttgcaaa atttcgggaa
    19981 ggtgtctcga agaatttaat ggtgggtaaa aaaaaccgac gcacactagc gtcggctctg
    20041 gcaggatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgc ggatcgtagc
    20101 cttcaatctc aaaatcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa
    20161 tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatgat
    20221 tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc gcccacttcc agatcacact
    20281 gctgcgccat catatgcacc agtaacgcgt agctggcaat gttaaacggc aggccgagga
    20341 aaacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact
    20401 ggaagaacgc atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg
    20461 aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc gtagtgatct
    20521 ggtcaatatg acgaccatcc ggcgttggcc aggcgcgcca ctgtttacca tacactggcc
    20581 cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat
    20641 aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc atggatgatg gaacgcaggt
    20701 ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac
    20761 caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca
    20821 ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc
    20881 tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa
    20941 tttgtcccat gctgatgtac tgcacccagg caccagtaaa ctgcgcgtcg ggctggcgga
    21001 aaaactcaac aatgatgcga aacgcgccgt aaccaatcag gaacaaacct gagacagctc
    21061 ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcact acaccttcca
    21121 gcaacagctc gtaaagctgt gatgggtggc gcggcagcac accgtaagta tcgaaaatgg
    21181 attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag ccagggaaca
    21241 gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc
    21301 ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca gagacctgga
    21361 agaaggaacg tttagtacgg cgggcgaaga taatcatcac cacgataacg ccaatcaggc
    21421 cgccgtggaa ggacatgccg ccgtcccaga cacggaacag atacagcgga tcggccataa
    21481 actgcgggaa gttgtagaac agaacataac caatacgtcc accgaggaag acgccgagga
    21541 agcccgcata gagtaagttt tcaacttcat ttttggtcca gccactgccc ggacgattcg
    21601 cccgacgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc
    21661 agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat
    21721 agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg
    21781 aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg
    21841 gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac cctccgcgaa
    21901 tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatgacga acttcggtcg
    21961 ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcaaaa tcaatgcgcc
    22021 acagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg cgatacccaa
    22081 gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacggagat
    22141 cgataccatg tatttccgct tcccgggcga tcattgccag agctcgtaac attgcaggat
    22201 gaaggctgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc agaatgtatt
    22261 gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca
    22321 taaataccat tgacggtact tccagcatga tgccgatacg tggtttggga atttcgtaac
    22381 cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct tcgtcaactt
    22441 catcgaggct ggtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcattagcac
    22501 gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc
    22561 cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg
    22621 ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc agcatcccct
    22681 gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg aatgggattt
    22741 cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcctca tgttccgggc
    22801 ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactga gcgggtaaat
    22861 tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc cgctgatatt
    22921 cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca tcaacgatca
    22981 gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta gggatcccca
    23041 gcgcacgtac catgatcgca gcatgggagt tggctgcacc atctcgcacg acaacaccga
    23101 ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct gccaccagaa
    23161 tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc
    23221 gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg cttagcgcgg
    23281 caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt
    23341 tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa
    23401 gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc
    23461 gaaactcgtt tgccgcttct tccagcgccc cggtcaatcg ttcgcgttcc agagccggat
    23521 ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt
    23581 cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg cgatattgcc
    23641 caaacaaggc agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca
    23701 ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta
    23761 caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt
    23821 cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg
    23881 gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc
    23941 gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat
    24001 cggccaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa
    24061 tatttaacgc ctcattcagg catggtgcgc tggctacctt ttcgactatt tcgcgcaggc
    24121 gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat gtgttttgtg
    24181 gttttggcgt attttcctgc agtgacatca ccacactcgc gaactctttc attaccctac
    24241 ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa cttacccatc
    24301 gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca tcgccgctca
    24361 ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac
    24421 gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa
    24481 cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg
    24541 attctccggg ggtgattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg
    24601 cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat
    24661 cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat tgtttcatac
    24721 tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac
    24781 tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct
    24841 tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct
    24901 gaataagccc gatataggca ttttaaaata ttcattatca gtcaatgcat tacatgtaat
    24961 ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
    25021 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
    25081 ctaaaaactg gagtttcgtc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
    25141 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
    25201 ccagatttgg aaaacctatt gcagtgctgc gcaactcgat tatcggcagc ctaaaaagca
    25261 atataaatca gagaattgaa caacacatgt ggaaaaagtt acactgcgaa tattcggcac
    25321 ataattgctg tttgtttttt aaccaaggta tcatgacatg tcccaacctc gcccactgct
    25381 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
    25441 attgggagaa ctggcggcac ttgccgggct ggttacgcca gagaatttaa aacgcgataa
    25501 gggctggatt ggcgtgttac aggagatctg gctaggtgcc agcgcaggga gtaaacctga
    25561 gcaagatttt gctgctctgg gtgtggaact taaaactatc cctgtggata gtcttggtcg
    25621 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg
    25681 ggaaaccagc cacgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga
    25741 gcgcagcatc ccgctggcgc agcgtcgcgt aggatccccg ttgctgtgga ccccgaatga
    25801 agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
    25861 tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc
    25921 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
    25981 acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat
    26041 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
    26101 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
    26161 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
    26221 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
    26281 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
    26341 ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt
    26401 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
    26461 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
    26521 gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt
    26581 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
    26641 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctttcttt cctgattctg
    26701 gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
    26761 ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa caaaaagaat
    26821 ccgctctgat actccgtacg ctctcctgct ggagggcgta ctcactcctt ttaccctcac
    26881 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
    26941 taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
    27001 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
    27061 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
    27121 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
    27181 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
    27241 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
    27301 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
    27361 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
    27421 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
    27481 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
    27541 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
    27601 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
    27661 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa
    27721 actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc
    27781 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
    27841 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccga gtattgtcac
    27901 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
    27961 ccagtatgaa ggggtcgaac tgctggccta ttcatgcctg ggtttcggca cgctgaccgg
    28021 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
    28081 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
    28141 tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc
    28201 cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg aaagtttgca
    28261 tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg tttatactta
    28321 tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
    28381 cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat gccaatgggc
    28441 acgaccggga tgcctaccat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
    28501 ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
    28561 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac gccaatcagc
    28621 atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag taccaccacg
    28681 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac taacttcgct
    28741 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtgggcgta
    28801 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
    28861 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcgc
    28921 catagcgagg tgcaggcatt caggaaactg cgggtcatgt tgatgagatt ccaggactgc
    28981 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
    29041 gccagtgcgc atgcggccag ggcgatgagg acatgccagt cagccagcac accaccggct
    29101 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agctttcact
    29161 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
    29221 gcaccaaccc ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
    29281 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
    29341 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
    29401 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
    29461 agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
    29521 gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
    29581 ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag
    29641 atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg
    29701 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct
    29761 ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
    29821 ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc
    29881 ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt cataccagcc
    29941 gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc
    30001 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
    30061 gataccagga accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
    30121 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcactcgg ttacaccata
    30181 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtag
    30241 tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt tggcgaaacg
    30301 cgcgtagtga ccgaggaaag tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac
    30361 caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt ctgcacctgt
    30421 aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga
    30481 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cgttcgccag
    30541 aatgctttta tggctatgga cgacgccttt tggatggcct tcagaaccgg aggtaaagag
    30601 gatcagcgcc tcttcttccg gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg
    30661 agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
    30721 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
    30781 gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca gcccttttac
    30841 cccagcagtg tagttcatca ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac
    30901 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
    30961 tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat aggagtctgg
    31021 ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcactcag
    31081 taaagattcg tacagagttt cacgcgggcg caccgccata cgcgcttcca tcattatttg
    31141 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcgacgcat ccggcatcgc
    31201 cacctgcgtt ggaggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
    31261 acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag gaataaccgt
    31321 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagcctgt
    31381 agtggtgatg cgtccttcag ggaaaatcac cactggtcga ccctgttcca ccagacgtac
    31441 cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
    31501 cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca ctggacgcac
    31561 aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag gcgtaattag
    31621 aacgcgctcg cccttcagtg cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg
    31681 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
    31741 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
    31801 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
    31861 aatctcacca atcaatacct ctgggattac cactttagca acctgaagcc aaacgccacc
    31921 agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt
    31981 gtcttactta tttgcgtttg gctcacattc ccacgatgaa aacacgcccc cccttgaacc
    32041 aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acccactaag gtattttcat
    32101 ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt
    32161 cattaataat tcacccaaag ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga
    32221 gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac
    32281 ggtcggtctg atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt
    32341 cgaacaggtg gcttatcaca ccggtaattt cttattgatt ggcaacggtt accacaacga
    32401 acaaaaagag cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt
    32461 ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa tgcccggtat
    32521 ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg
    32581 ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta
    32641 tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc
    32701 ccttgctgaa agtggtattg cggccaatga ccggctggtg acatttggcg aaccagacga
    32761 aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggaagaaatt tcactgcggt
    32821 agcctgttat aacgattcaa tggcggcggg tgcgatgggc gttctcaatg ataatggtat
    32881 tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt
    32941 gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgaccc aggctgccga
    33001 actggctttg gcgctggcgg ataatcgccc tctcccggaa atcactaatg tctttagtcc
    33061 gacgctggta cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac
    33121 cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac
    33181 ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc gaacggctat
    33241 tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac
    33301 ctgccttcac ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga
    33361 catcgcccga ttcacataac ggtccggcga cgacggtttc caccgttggc gcgtgttcca
    33421 gagaacgacc atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc
    33481 gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctcccc atttgtttga
    33541 cgctccgcac ctgagtaatt aatacgccag actgcgctac caggaagcga cccggttcaa
    33601 tttccagttt cacagggtgg cccaaatggc gggcgatttg ctcacgcgcg gcattccaca
    33661 gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga acagaaagcc
    33721 caccgcccgc agaaatagcc tgtaaatcct gaccgaattc gatgacctga cgcaccatag
    33781 caccacacac ctgttccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc
    33841 cgaccagctg cagatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc
    33901 agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa
    33961 accccggatt aacgcgcagc catacccgat gccctggcga aaccgggccc agttggtcga
    34021 gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg
    34081 cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac
    34141 ccgccgccaa cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct
    34201 cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccacat
    34261 caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg
    34321 ggcagccaaa ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc
    34381 tgaacagtga atgtggcata acaaactcca gataagtgct tttttatgat tacgccacat
    34441 cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga tatggattac
    34501 cggattgcga gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg
    34561 cggtaatgac cgccggaagc ctgactgagg cggcacacct gctacacacc tcacagccaa
    34621 ccgtcagccg cgaacttgcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg
    34681 tacgtgggcg attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat
    34741 cctggtacgg actggatcga attgtcagcg ctgcagaaag tctgcgcgag tttcgccagg
    34801 gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc
    34861 aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc
    34921 tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgag acactccata
    34981 cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc
    35041 cgggtcatcc gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaact
    35101 acatcagcct ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac
    35161 atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcaatc tgcgcaatgg
    35221 tacgggcggg ggtaggtgtt tcggtggtta acccgctcac cgcactggat tatgcggcaa
    35281 gcggtttagt ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc
    35341 ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag tgagcattta caagcggggt
    35401 taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc
    35461 gacagcatcc tcggcatgga tcgccgcagt atcaaacaca ggcaaaacac tgcgctcttc
    35521 tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc
    35581 aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca
    35641 cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca
    35701 attgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg
    35761 cgtacccagc agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc
    35821 cgcaatgtgt aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat
    35881 cgtattggta cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc
    35941 agccagaatg tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg
    36001 aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat
    36061 gccttcattt atcaaacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa
    36121 accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa
    36181 cgagacaaac gcctcaacgg ccaagtgccc aatctctatt aacgaaaaaa gggccggatg
    36241 tacagcacat ccggcccgtg aaatcagatg ccgatatttc tcaacttctc gcctgccatc
    36301 agtttgcgtt cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagtg
    36361 atgcccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg
    36421 ctgtcgagca gcgtcaggaa ggtcgcgccg ataatcatat tcgacaccca gttcgtggtg
    36481 gtcgaacagg taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc
    36541 cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca
    36601 gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc
    36661 agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgccct
    36721 gctttatcta ccgtaaacac cgcaataaag gtggcgaaca taaaggtcag ccctacgacc
    36781 agagtcgtaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc
    36841 gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg
    36901 agaaacacag cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt
    36961 aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc
    37021 agcatacgca atacttcttc cgcctcaata tgacgcccct tttccgccaa ccagcgcggg
    37081 ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc aagaaccccc
    37141 aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg
    37201 atgccgagtg tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt
    37261 gccatttcag aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc
    37321 accacacgag cggcgattaa catctctaca ctggtcgcaa aagcggaccc tatagaaccg
    37381 agtacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc
    37441 caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactaac cacccattcc
    37501 tgcaaatgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg
    37561 ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc
    37621 atacgccgcg tatcccgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc
    37681 attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca actcacgcag
    37741 gtgtcaggtc ggaaacagca taaatatgga ttaaattgct gcgacatgtc gttatgtgat
    37801 ggatattcca attttcaaat taagttgaat tattgagatt attattaacc acctaatttt
    37861 acagcagata aaattcataa agttcattaa ttgataatta atatggatta tttcataacc
    37921 atgatatgga ttatgatgat ctacaggtat aaaaaaccct gccatgcggc agggtcataa
    37981 aagtaagaag aatgaattaa cgcgccagcc aaccgccatc cacggcaatg gtataaccat
    38041 tcacataatc tgaagcgctg gaggcaagga acactatcgg ccccatcagg tcactcggca
    38101 gtccccaacg accagctgga atgcggtcga gaatttccgc gctacgttgt tcatctgccc
    38161 gtagttgttg agtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt
    38221 tgtgtttagc ccattcgttc gccatcaatc gcgtcacacc catcacgccg ctttttgatg
    38281 cggtataaga aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa
    38341 tcttgccgcc attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata
    38401 cgctcttgat attcaggttc atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt
    38461 cttcgcggcg aatcaatccg gcgttattca ccaggatatc aatatgacca aattcagcta
    38521 ccgcgcgatc cagcagtcca ggaatgccat caatctttcg cagatcggcg gtcaggctta
    38581 aaaaacgacg ccccagcgcc gtgacctgct cgatggtttc agtcggttca acgatgttaa
    38641 tgccaacaat gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag
    38701 tatcacaacc agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa
    38761 caatacctta ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat
    38821 atcatcaaag acctggtttt caccaaccat gccccagata aaggtataag ctttggttcc
    38881 gacgccggaa tggatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat
    38941 gtgacgcgtt tcttgcggtt gccccatcat gtggaaaacg caggcgtcat catccatatt
    39001 gaaatagaaa taaacttcca tccggcgctc gtgggtgtga cacggcatgg tgttccacaa
    39061 gttacctgga gccagctccg tcagccccat actcaattgg caggtttcca gcacatccgg
    39121 gacaaaatat ttgttaatag tgcgacggtt actggtgagg ttatcgccta acgtgactgg
    39181 agatacttcg tccggggtga cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt
    39241 gtaataaaac tttgccggag tggcggtatc aatactggca aagacaactt cttttgcacc
    39301 tttaccaaca tacagggcat cgcggtgacc gatttcatag cattggccat cgacagtaat
    39361 cgtaccggca ccgccaatat tgataacacc taactcgcga cgttcaagga aatagcgtac
    39421 gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc
    39481 gccaacaata attcggtcaa tgtggctgta aaccatggtg tactcatcgg cgacaaatac
    39541 cttttcaacc aaaaattcat tgtgcagccc ttgggtatcc agcgtttttg cgtgcgcact
    39601 gtggatgctc tgtcttacgt ccacatcaac ctccgataat cagtgaagcg tgaaaataga
    39661 aaacgatagt gaaacactgt ttcgttttct ctataaaggg atgatatgcc catgggctag
    39721 ggttttcaat taaaaataaa acaatgtttt atttttaagt taaggattaa aaaaagtgca
    39781 gtgtcgatca cgaatatgtc atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc
    39841 gacttgccag ccatcattat tcaacagatt aatgacggca gatatttatc ggtaaggagg
    39901 aaatgcagag ctactcgtca cgttcaatgg tcaatgccac gccctggccc ccgccgatac
    39961 aaagcgttgc cagccctttg cgggcattac gtttcaccat ttcatgaacc agagaaacca
    40021 ggattcggca accggaagcg cctatcgggt gaccgagtgc gatcgcgcca ccattgacat
    40081 tgacccgacg ctcatcccac tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg
    40141 cttcattagc ctcgataaga tcgacatcag ccaactgcca gcctacacgc tccaggcaac
    40201 ggcgggtcgc atacaccggc gcaattccca tcaatgccgg atctacacca acgctggcaa
    40261 atgcgcggat gcgggccagc acgggtaaat tcaacgctcg tgctttggct tcgctcatca
    40321 tcattaccgc agctgcgcca tcgtttatgg atgatgcatt ccccgcggtc acagaaccga
    40381 gactatcaaa tgaaggattt aaacgggcta aaccttctgc actggcgtca gtgcatggct
    40441 gttcatcggt atcaacaacc aacgtctgac cgttactttg gatcattacc gggacgatct
    40501 catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt
    40561 aagcatcctg caactgacgg ctgatgccat attcgcgagc cagattttcg gcggtgacac
    40621 caatatgata atcattgaag gcatcccaca acccatcatg caccagactg tcaaccaact
    40681 ggctattgcc aagctgtgca ccggtgcggc tatcagtcag aacatgtggt gcgcggctca
    40741 tgttttcctg gccaccggcg atgacaatat cagcctcgcc acactgtatc gcctgagtag
    40801 ccagatgcag tgctttaagc ccagaaccgc aaacgtcatt aatagtgatt gcagaaacgc
    40861 tattaggaag accaccttta atagccgatt gccttgccgg attctgccct gcccctgcag
    40921 tcaacacctg accaagaatt acttcatcca ccgcatatgc aggaacgccg gtacgttcta
    40981 ttaacgcttt cacgaccaaa ctaccaagtt ccacggcgga atgacccgct aacgcaccac
    41041 gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac aacgtctttc atcacaacct
    41101 cgcaccaatt aacagtgcaa tagtaaatga ttgttaatta cagttatctt aattgtttaa
    41161 aaaaagtgat ttttatcaca aaggaaatat gcctgagcag cagtcagaga cataactggc
    41221 acgtaaggtt tgcaaccact aacccaccaa tagaggggta gatggggcgt taatctccca
    41281 tacttaacct ggtttatggt aaattgccct ccattttgtt taatttgtag atgatacgtt
    41341 cagataatgt ctaatatttg gtctaaagaa gaaactctgt ggagtttcgc gctctacggc
    41401 acagccgttg gtgcaggcac gctcttcctt cctattcagt taggttcggc aggggctgtg
    41461 gtcctgttta ttactgctct ggtcgcctgg cctttaacat attggccaca taaagcttta
    41521 tgccagttca tcctctcatc gaaaacatca gcaggtgaag ggataacggg cgcggtaaca
    41581 cactactatg gcaagaagat tggtaatctg attaccacgc tgtacttcat cgcctttttt
    41641 gtcgtcgtgt tgatatatgc agtggcaatt accaactcac ttacagaaca gctggcaaag
    41701 catatggtta ttgatcttcg catccgtatg ttggtgagtc tgggtgttgt attaatcctg
    41761 aatctcattt ttctgatggg acgccatgca accattcggg taatgggttt tttggtattc
    41821 ccattgattg cctatttctt atttctttcc atttaccttg tcggtagttg gcaacctgat
    41881 ctattaacga cccaggtaga gttcaatcag aatccccttc accagatatg gatatcgatt
    41941 cccgtgatgg ttttcgcctt tagccatacg cccattattt ctacgtttgc catagacaga
    42001 cgtgaaaaat atggcgaaca cgctatggat aaatgcaaaa aaattatgaa agtcgcttat
    42061 ctcatcatct gcataagtgt actgttcttt gtctttagct gcctgctttc tattccacct
    42121 tcgtatattg aagcggctaa agaagaaggg gtcaccattt tatcggcgct ttctatgctg
    42181 ccgaacgccc cagcatggtt gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg
    42241 aaatcattcc tgggtacgta ctttggcgtt attgaaggtg ccacggaggt cgtcaaaaca
    42301 acactacagc aggttggtgt aaagaaaagt cgtgcattta accgcgcact atcaattatg
    42361 ttggtatcgc tgattacctt cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt
    42421 tatgcgatca gcggcccgct cattgccatg atacttttca tcatgcctac gttgtcaacg
    42481 tatctgatcc cggcgcttaa accctggcgc tccatcggaa atctgattac gctgatcgtg
    42541 ggtatcctgt gcgtatcggt aatgttcttt agct
//
LOCUS       DAJTMK010000011        42560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTMK010000011 DAJTMK010000000
VERSION     DAJTMK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42560
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.011"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..311
                     /locus_tag="OO135_000515"
     CDS             <1..311
                     /locus_tag="OO135_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5396475.1"
                     /translation="AFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGGAVGAAAGSMV
                     NDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRIQPNTKCPEKS
                     "
     gene            327..866
                     /locus_tag="OO135_000516"
     CDS             327..866
                     /locus_tag="OO135_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5396476.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQVAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(961..2538)
                     /gene="guaA"
                     /locus_tag="OO135_000517"
     CDS             complement(961..2538)
                     /gene="guaA"
                     /locus_tag="OO135_000517"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5396477.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2607..4073)
                     /gene="guaB"
                     /locus_tag="OO135_000518"
     CDS             complement(2607..4073)
                     /gene="guaB"
                     /locus_tag="OO135_000518"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5396478.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGCLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4235..5605
                     /gene="xseA"
                     /locus_tag="OO135_000519"
     CDS             4235..5605
                     /gene="xseA"
                     /locus_tag="OO135_000519"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5396479.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5602..5817)
                     /locus_tag="OO135_000520"
     CDS             complement(5602..5817)
                     /locus_tag="OO135_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5396480.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGELIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5886..7358)
                     /gene="der"
                     /locus_tag="OO135_000521"
     CDS             complement(5886..7358)
                     /gene="der"
                     /locus_tag="OO135_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5396481.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQELSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7476..8654)
                     /gene="bamB"
                     /locus_tag="OO135_000522"
     CDS             complement(7476..8654)
                     /gene="bamB"
                     /locus_tag="OO135_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5396482.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8665..9285)
                     /gene="yfgM"
                     /locus_tag="OO135_000523"
     CDS             complement(8665..9285)
                     /gene="yfgM"
                     /locus_tag="OO135_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5396483.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9303..10577)
                     /gene="hisS"
                     /locus_tag="OO135_000524"
     CDS             complement(9303..10577)
                     /gene="hisS"
                     /locus_tag="OO135_000524"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5396484.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10688..11806)
                     /gene="ispG"
                     /locus_tag="OO135_000525"
                     /gene_synonym="gcpE"
     CDS             complement(10688..11806)
                     /gene="ispG"
                     /locus_tag="OO135_000525"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5396485.1"
                     /translation="MHNQAPIQRRKSTRIYVGYVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11833..12846)
                     /gene="rodZ"
                     /locus_tag="OO135_000526"
     CDS             complement(11833..12846)
                     /gene="rodZ"
                     /locus_tag="OO135_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5396486.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13131..14285)
                     /gene="trmG/rlmN"
                     /locus_tag="OO135_000527"
     CDS             complement(13131..14285)
                     /gene="trmG/rlmN"
                     /locus_tag="OO135_000527"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5396487.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14435..14866)
                     /gene="ndk"
                     /locus_tag="OO135_000528"
     CDS             complement(14435..14866)
                     /gene="ndk"
                     /locus_tag="OO135_000528"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5396488.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15015..17318)
                     /gene="pbpC"
                     /locus_tag="OO135_000529"
     CDS             complement(15015..17318)
                     /gene="pbpC"
                     /locus_tag="OO135_000529"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114026.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5396489.1"
                     /translation="MLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARVVVA
                     QDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARAAWQ
                     DLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNR
                     APFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAARNK
                     VLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAG
                     LQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMVNAI
                     RSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEALVR
                     SLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYT
                     AFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWK
                     TGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVDQFGFASAVPLLNQVNNILLSRSA
                     NLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEGING
                     IRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASESRAVRLPPASTSCPPYG
                     HDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTL
                     HLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17328..22288)
                     /gene="yfhM"
                     /locus_tag="OO135_000530"
                     /pseudo
     CDS             complement(17328..22288)
                     /gene="yfhM"
                     /locus_tag="OO135_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
     gene            22495..23340
                     /gene="sseA"
                     /locus_tag="OO135_000531"
     CDS             22495..23340
                     /gene="sseA"
                     /locus_tag="OO135_000531"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5396490.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(23833..24609)
                     /gene="sseB"
                     /locus_tag="OO135_000532"
     CDS             complement(23833..24609)
                     /gene="sseB"
                     /locus_tag="OO135_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5396491.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24751..26034)
                     /gene="pepB"
                     /locus_tag="OO135_000533"
     CDS             complement(24751..26034)
                     /gene="pepB"
                     /locus_tag="OO135_000533"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5396492.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26212..26412)
                     /gene="iscX"
                     /locus_tag="OO135_000534"
     CDS             complement(26212..26412)
                     /gene="iscX"
                     /locus_tag="OO135_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5396493.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26424..26759)
                     /gene="fdx"
                     /locus_tag="OO135_000535"
     CDS             complement(26424..26759)
                     /gene="fdx"
                     /locus_tag="OO135_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5396494.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26761..28611)
                     /gene="hscA"
                     /locus_tag="OO135_000536"
     CDS             complement(26761..28611)
                     /gene="hscA"
                     /locus_tag="OO135_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5396495.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGADVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28628..29143)
                     /gene="hscB"
                     /locus_tag="OO135_000537"
     CDS             complement(28628..29143)
                     /gene="hscB"
                     /locus_tag="OO135_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5396496.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTPFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29239..29562)
                     /gene="iscA"
                     /locus_tag="OO135_000538"
     CDS             complement(29239..29562)
                     /gene="iscA"
                     /locus_tag="OO135_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5396497.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29579..29965)
                     /gene="iscU"
                     /locus_tag="OO135_000539"
     CDS             complement(29579..29965)
                     /gene="iscU"
                     /locus_tag="OO135_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5396498.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29993..31207)
                     /gene="iscS"
                     /locus_tag="OO135_000540"
     CDS             complement(29993..31207)
                     /gene="iscS"
                     /locus_tag="OO135_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5396499.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31319..31807)
                     /gene="iscR"
                     /locus_tag="OO135_000541"
     CDS             complement(31319..31807)
                     /gene="iscR"
                     /locus_tag="OO135_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5396500.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32077..32817)
                     /gene="trmJ"
                     /locus_tag="OO135_000542"
     CDS             complement(32077..32817)
                     /gene="trmJ"
                     /locus_tag="OO135_000542"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5396501.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32936..33739
                     /gene="suhB"
                     /locus_tag="OO135_000543"
     CDS             32936..33739
                     /gene="suhB"
                     /locus_tag="OO135_000543"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5396502.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33884..34738
                     /locus_tag="OO135_000544"
     CDS             33884..34738
                     /locus_tag="OO135_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5396503.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWLLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34929..36224
                     /gene="csiE"
                     /locus_tag="OO135_000545"
                     /pseudo
     CDS             34929..36224
                     /gene="csiE"
                     /locus_tag="OO135_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
     gene            complement(36216..37355)
                     /locus_tag="OO135_000546"
     CDS             complement(36216..37355)
                     /locus_tag="OO135_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5396504.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37515..38405)
                     /gene="hcaR"
                     /locus_tag="OO135_000547"
     CDS             complement(37515..38405)
                     /gene="hcaR"
                     /locus_tag="OO135_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5396505.1"
                     /translation="MELRHLRYFVAVAQALNVTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASITA"
     gene            38541..39902
                     /locus_tag="OO135_000548"
     CDS             38541..39902
                     /locus_tag="OO135_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5396506.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVCALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39899..40417
                     /locus_tag="OO135_000549"
     CDS             39899..40417
                     /locus_tag="OO135_000549"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5396507.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40417..40737
                     /locus_tag="OO135_000550"
     CDS             40417..40737
                     /locus_tag="OO135_000550"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5396508.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40734..41546
                     /gene="hcaB"
                     /locus_tag="OO135_000551"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40734..41546
                     /gene="hcaB"
                     /locus_tag="OO135_000551"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5396509.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41556..>42560
                     /locus_tag="OO135_000552"
     CDS             41556..>42560
                     /locus_tag="OO135_000552"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5396510.1"
                     /translation="MKEKTIIIVGGVQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLLPPPWFWSDQYSDNLQFIGDMRG
                     D"
BASE COUNT        10045 a        11757 c        10713 g        10045 t
ORIGIN      
        1 aagccttcgg cgcgcttatt ggcgcagtcg ctggcggtgt tatcggccac aacgtcggtt
       61 ctggcagcaa ttccggaacg acggcagggg cagttggcgg cggagctgta ggcgcggcag
      121 cgggttctat ggtgaatgat aaaaccttag tggaaggtgt ttctttaaca tataaggaag
      181 gcaccaaagt gtatacctct acacaggtgg gtaaagagtg ccagtttacg acaggtttag
      241 ccgttgttat taccacaacg tataacgaaa cgcgtattca gccaaatact aaatgtcctg
      301 aaaagagcta ataatcagga ggagtcatga agaaagtttt tctttgcgcc atcttggcct
      361 ccttaagcta tccggctatc gcctcatcat tgcaggatca actctctgct gtagcagaag
      421 cggaacagca aggtaaaaat gaagagcaaa ggcagcatga cgaatgggtc gcggagcgca
      481 acagggaaat ccagcaagag aagcaacgtc gcgcaaatgc ccaggtcgcc gctaacaaaa
      541 gagcggcaac ggcagcagca aataagaaag ctcgtcagga taaactggac gccgaagcct
      601 ctgcggacaa aaaacgcgat caaagttatg aagatgagct acgcagctta gagattcaga
      661 aacaaaaact ggcgctggca aaagaagaag cccgcgttaa gcgagaaaac gaatttatcg
      721 atcaggaact gaagcacaaa gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcca
      781 acagaaatat gactgaaggc ggtcgcgatc tgatgaaaag cgtgggcaaa gcagaagaga
      841 acaaatcgga cagctggttt aattaatcga tgttagtaac ttcaagccta tgattcttga
      901 agataaaaaa ccctctgtag taacagaggg ttttgttcat tcatagtgca gggattaaaa
      961 tcattcccac tcaatggtag ctggcggctt gccgctgatg tcatacacca cgcgggaaat
     1021 accgttcact tcattgataa tgcggttgga aacgcgaccg aggaaatcgt acggcagatg
     1081 cgcccagtgt gcggtcataa agtcgatggt ttcgacagca cgcagagaga caacccagtc
     1141 atacttacga ccatcgccca ttacgccaac ggaacgtacc ggcaggaaca cagtgaacgc
     1201 ctggctgact ttgttgtaca ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc
     1261 acggcgcagc aggtcacagt actctttctt cacttcaccc agcacacgaa cgccaaggcc
     1321 tggtcccggg aacgggtgac ggtacagcat gtcgtacggc aggcccagct ccagaccaat
     1381 cttacgcact tcgtctttga acagctcttt cagcggttca accaggccca tcttcatctc
     1441 tttcggcagg ccgcccacgt tgtggtgaga tttgatgacg tgtgctttac cggttgcaga
     1501 agccgcagat tcgataacgt cagggtagat ggtgccctgc gccagccact tcacgtcttc
     1561 cagtttcagc gcttcttcat cgaacacttc aacgaaaacg cgcccgatga ttttacgttt
     1621 tgcttccgga tcgttttcgc cagccagcgc tgacaggaag cgatcttctg ccggtacgtg
     1681 aacaatgttc agaccaaagt gatcgccaaa catatccaga acctgctctg cttcgttgag
     1741 acgcagcagg ccgttgtcga cgaatacgca agtcaggttt ttaccgatag cgcggtgcag
     1801 cagcattgcg gttacggagg aatccacacc accagagagg ccgaggatga ctttatcgtc
     1861 gcctacctgc tcgcggatgc gagctacagc atcgtcgata attttcgctg gcgtccacag
     1921 ggcttcacac tggcagatat cacgcacaaa acgctccagc atgtgcatac cctggcgggt
     1981 gtgagtcact tccgggtgga actgtacgcc atagaagcgt ttttcttcgt tagccataat
     2041 ggcaaacggg cagctttcgg tgctggctac ggtgacgaag tcggacggga tagcggtaac
     2101 tttatcgccg tggctcatcc agacatcgag cagcggttta ccgtctgcgg tcagcgcatc
     2161 ttcgataccg cgaaccagtg cgctgtcgtt tacgacttca acctgcgcgt agccaaattc
     2221 acgttcgtta gaggcttcaa cgtgaccgcc caactgcatt gccatggtct gcatgccata
     2281 gcaaacgccg aataccggta cgcctgcttc aaagacatac tgcggcgcac gcggactgtt
     2341 ttcttcagta gtactttccg ggccgccgga aagaataatg ccgcttggat tgaagtcacg
     2401 aatttgtgct tctgtcacat cccacgccca cagttcgcag taaacaccca gctcacgcac
     2461 gcggcgcgca accagttgag tgtactgaga accgaagtcc agaatgagga tgcgatgctt
     2521 atgaatgttt tccgtcattg acgcttattc cgaggcaagt gaaacagata atataaatcg
     2581 cccgacatga agtcgggcga agagaatcag gagcccagac ggtagttcgg ggactcttta
     2641 gtaatggtca cgtcgtgaac gtggctttcc tgaatgcccg caccgctgat acgtacaaac
     2701 tccgctttag tacgcagttc gtcgatagta ccacagccgg tcaggcccat acaggagcgc
     2761 aggccgccca tctgctggtg aatgatctct ttcaggcaac ctttataggc tacgcgacct
     2821 tcgatacctt ccggcaccag tttgtcggca gcgttgtcac tctggaagta acggtcagaa
     2881 gaacctttgg acatcgcacc cagagagccc ataccacggt aagatttgta agaacggccc
     2941 tggtagagtt caatttcacc cggagattct tcagtacccg ccagcatgga acccaccatt
     3001 accgcgcttg cgccagcggc gatagctttg gcgatgtcgc cggagaagcg aataccacca
     3061 tctgcgataa ccggaatacc tgtgccttcc agcgcttcaa ctgcgtcagc aacggcagtg
     3121 atctgcggaa cacctacgcc agtaacgata cgagtcgtac agatagaacc agggccgata
     3181 ccgactttaa ccgcgctgca accagcttct gccaaagcgc gtgcacctgc agctgttgcc
     3241 acgttgccgc cgatgatttg cagatccgga tatttagcac gggtttcacg gatacgctgc
     3301 aaaacgcctt ctgagtggcc gtgggaggag tcgatcagca gaacgtcaac gccagcggca
     3361 accagcgcgt caacacgctc ttcgttaccc gcacctgcgc caactgcagc accaacacgc
     3421 agacggcctt gctcgtcttt acaggcgttc ggtttacgtt ccgctttctg gaagtctttc
     3481 acggtgatca tgccgatcag gtggaattcg tcatcaacca ccagcgcttt ttcaacgcgt
     3541 ttttcgtgca tttttgccag caccacttca cgggcttcac cttcacgcac ggtgaccaga
     3601 cgctctttcg gcgtcatgta aacgctaacc ggctggttca ggtcggtaac aaaacgcacg
     3661 tcacgaccgg tgataatacc caccagttcg ttttcttcgg taacaaccgg atagcccgca
     3721 aaaccgttac gctcggtcag ttctttcact tcgcgcagcg tcgtggttgg cagaacagtc
     3781 tgcggatcag tcaccacacc agattcgtgt tttttcacac ggcgaacttc ttctgcctgg
     3841 cgttcaatgg acatgttttt gtggataaag ccgataccgc cttcctgagc cagagcaata
     3901 gccaagcgcg cttccgttac ggtatccatt gctgcggaaa gcatagggat attcagacga
     3961 atagttttcg tcagctgggt gctgaggtca gcagtattcg gcagaacggt agaatgagca
     4021 ggaacgagga gaacgtcgtc aaacgtcaga gcttctttag cgatacgtag catgggcaat
     4081 atctcgacca gagtggttaa taaatattgc cgcggcatta tacagagcgt aaccgattgc
     4141 atctacccct ttttgcaaaa aatgcttgct atccccgaag ggcgggttac tatcgactga
     4201 ataacctgct gatttagaat ttgatctcgc tcacatgtta ccttctcaat cccctgcaat
     4261 ttttaccgtt agtcgcctga atcaaacggt tcgtctgctg cttgagcatg agatgggaca
     4321 ggtttggatc agcggcgaaa tctctaattt cacacaaccg gcttccggtc actggtactt
     4381 tacactcaaa gacgacaccg cccaggtacg ctgcgcgatg ttccgcaaca gcaaccgccg
     4441 ggtgaccttc cgtccacagc atggacaaca agttttagtt cgcgccaata ttacgctcta
     4501 cgagccgcgc ggtgactacc agataatcgt tgagagtatg cagccggccg gtgaagggct
     4561 gctgcaacag aagtacgaac agctcaaagc gaagttgcag gctgaaggtt tgttcgatca
     4621 gcaatacaaa aaaccacttc cctcccctgc gcattgcgtt ggtgtgatca cctcaaaaac
     4681 cggtgctgcg ctacatgata ttttgcatgt gttaaaacgt cgcgatcctt ctctaccggt
     4741 gatcatctac cccaccgccg ttcagggcga tgacgcaccg gggcaaattg ttcgcgccat
     4801 tgaactggcg aatcagcgca atgagtgcga cgtgttgatc gttgggcgcg gcggcggttc
     4861 gctggaagat ttatggagtt ttaacgacga acgcgtagcg cgggcgattt ttgccagccg
     4921 cattccggtc gtcagcgccg tcgggcatga gacggatgtg accattgccg attttgttgc
     4981 cgatctgcgt gcgccaacgc cgtctgccgc cgctgaagta gtgagccgta atcagcaaga
     5041 gttactgcgc caggtgcaat cgacccgtca acggctggag atggcgatgg attattatct
     5101 cgccaaccgc acgcgtcgct ttacgcagat ccatcaccga ttacagcaac agcatccgca
     5161 gctccggctg gcacgccagc aaaccatgct tgagcgcctg caaaagcgga tgagctttgc
     5221 gctggaaaat caacttaagc gtaccgggca acagcagcag cggttaacac agcggctgaa
     5281 tcagcaaaat ccacagccga agattcatcg cgcgcaaacg cgcattcagc aactggaata
     5341 tcgtttagca gaaaccctgc gcgtacagct tagcgccacg cgtgaacgtt tcggtaatgc
     5401 agtaacgcac ctcgaagccg taagcccact gtcaacgctg gcgcgtggat acagcgttac
     5461 tactgctact gacggcaatg tactgaaaaa agtgaagcaa gttaaagcgg gtgaaatgct
     5521 aaccacacgt ctggaagacg gctggataga aagtgaagtt aaaaacatcc agccagtaaa
     5581 aaaatcgcgt aaaaaggtgc attaagccag cacgaattca acgcgttttt tcgatatcaa
     5641 gccatgcccg tgctgacaga agtaatctac tgcaccgcag gcttttaaca cctgcaacgg
     5701 ttgatggcag tctggacaaa gagctttcat ttcaataagt tccccgcagg atgggcaacg
     5761 cgcatggccg ttatcctgat caagcacatg ctgacattgt ggacaatgca gttccatatg
     5821 aattcctccg gtagccatcc ctctacattc atagagggaa tggcagataa aatacttacg
     5881 gataattatt tatttttctt gatgtgcttc atcagacgct tacgtttacg catctgggtt
     5941 ggcgtcaggg tgttacgctt gttcgcatac gggttttccc cttctttgaa ctgaatacga
     6001 atcggcgatc ccattacgtc cagcgatttg cggaagtagt tcatcaagta gcgcttgtag
     6061 gaatcaggca ggtctttcac ctgattaccg tgaatcacca caatcggcgg gttataacca
     6121 ccagcgtggg catatttcag cttcacacga cgaccgcgta ccagcggcgg ttggtgatct
     6181 tcaacagcca tcgtcatgat gcgcgtcagc atagaggtcc ccacacgacg ggtggagctg
     6241 tcatacgctt cacgtactga ttcaaacaag ttaccaacac cactgccgtg taaggcagag
     6301 ataaagtgca cacgagcaaa atcgataaag cccagacgga agtccagcgt ttctttcacc
     6361 tgctctttca cttcctggct caggccatcc cacttattca ccacaatgac aagtgagcgc
     6421 ccactattga gaataaagcc cagcagcgag agctcctgat cggaaatacc ttcgcgcgca
     6481 tcaatcacta acatcaccac gttggcatct tcaatggcct gcaacgtttt gattacggag
     6541 aatttctcta cagcatcggt gattttgccg cgtttacgta cgccagcggt gtcaatgagc
     6601 acatactcac gtccatcgcg ttccattgga atgtagatac tgtcacgcgt cgtgccaggc
     6661 atgtcataaa caacaacgcg ctcttcacca agaatacggt tagtgagtgt agacttacct
     6721 acgttcggac gacccacaat cgccagtttg atcggcagac tttgcgggtc gaagtcgtct
     6781 tcctcttctt cttcgccgtt ctcttccgct tcaaattgcg cccagtattc agcgtcttcg
     6841 tcgacttcct cttgcggtgc gagatcttcc atccacggca gcagcacatg ctccagcaga
     6901 cttaatacgc cacgaccgtg agacgcggcg atcgggtaga tttcacctaa gccaagcgag
     6961 tagaaatcaa ccactgcctg atcgggatcc agaccgtcag ttttgtttgc caccaagaag
     7021 gtcggttttt cacgggagcg cagatgtttg gcaatcgctt catctgccgg catcaggccc
     7081 gcgcgcgcat ccaccataaa cagtacgacg tccgcttctt caatcgccag cagcgactgt
     7141 tccgccatgc gggtttctac accgtcttct gtgccatcaa tcccgccggt atcaatacag
     7201 ataaactcac ggccttcaat ttccgcacga ccgtacttac ggtcacgagt cagacccggg
     7261 aaatccgcaa ccagcgcatc tcgggtgcga gttagacggt taaataacgt ggattttcct
     7321 acgttagggc gcccgacaag cgcgaccaca ggtaccatgt ttaaagcctc atttttataa
     7381 atcatcagac aacgcacgct atattcgcgt cgttgttaaa aacaggaaaa cggcccctgt
     7441 ccaggagccg ttttcaaagt gaacgacaga gacgattaac gtgtaataga gtacacggtt
     7501 ccgtcttttg cctggatcag cagtttgccg tcagcggcaa ccggttcagt ctggaaaccg
     7561 gaactatcaa ctttttgctg ggcaacgaaa cgaccatctt cgacattaat ccagtgcaga
     7621 taaccttcac tgtcaccgac caccaggttg ccatcataca gcaccggaga agtcagcagg
     7681 cgatgcaaca gatcgctttg tgtccacagc gtaacgccgc catcaatggt caacgccatc
     7741 acccggtcat tttgatcgac cagatagatg cgattgccgt cgacgatgaa atcattcacc
     7801 gaacccagtt cgcgtttcca cataatctga ccactgcgca gatcaagcgc cgtcaggtta
     7861 ccattatagg ccagcgcgaa aacaacgccg ttaacaacga cgggagtcgt gtcaacatcg
     7921 ctcagacggt caatttcggt agaaccggtc gcctgggaaa tacgctgctg ccaaatcatc
     7981 tggccctgtt ccatcagcac tgcgctgacg cgaccattat cgccccccac gacggccgca
     8041 ccaaaagccg ttgccggcgc agactcgcca cgcaaagaga gcgaaggcat atcgaggtta
     8101 actgtccatt tgacagcgcc gtcagcttcg ttcagcgctt gtaactgacc gttactggtg
     8161 tggattaaca ccagaccgtc gctgaccacc gggcgcgaaa gtgcttcacc cgcgacttta
     8221 gtttgccatg ccacagtacc atcgctggta ttcagcgcgt aaacctgcgc cttttcgctg
     8281 ccaatgtaga catgcccacc agacacggtc acaccgccag aaagtaatgc aggctcttta
     8341 gagaaccagc catctttctc ggccaggctg acagaccaga tttctttgcc atcatccgca
     8401 ttcagcgctt ttaccaaacc agcgcggtcc gctgcataga caacgttgtc cgccagtgcc
     8461 ggatgaagat tggaatagaa gttgccaatg ccgctaccaa cggaagtact ccacgccgtg
     8521 gtcggcgtaa actggttttc aacggttggc aatggggaca tctttaccac atcttcttcg
     8581 ctgttaaaca gcgaacagcc gcttaaaagg gtaacggaaa gcagtcctgg cagcagtaat
     8641 ttacgcaatt gcatcgggtc cctctcagat ggacaaatta ttaattttca tctgcatcat
     8701 ttcgctcagt gccggagtaa catcgctttt cacgcctgct tcccatgcac tacgcgcacc
     8761 ttgcttatca cctttgctca gcaatgcttc accacgcagg tcggcaacaa tggcagccca
     8821 cccttcacct ttgatggtat caagggtttt cagcgcggca tcagcctgct tgagctgtac
     8881 ctgaacgcga gcaagacgca gatttatcac ggctttgaga ttttcatcgc tcgtgtctgc
     8941 cagcccctgt tgtaactggg cggcagcttt ctctagttca tttttgtcaa caaattgctg
     9001 cgcaagttcc aaagaagcca gcgcaccata agtattttta ttttcagcag caaatttttc
     9061 cgccgccggg atgctatccg gtttgccttc gctcactgcg gtaaccgcat tttgataggc
     9121 aagagaagcg gagcgtgcag aatcaacctg atggctgttc cagtagcgcc agccaatcag
     9181 tgcgccaacg cccaaaatca ccccaacagc cagtgctttg ccattttcag caaaaaagcg
     9241 tttaaccgct tctacctggt cgttttcgtt ctcgtaaatt tccacgctgt ccttctcctt
     9301 ccttaaccca gtaacgtgcg caaatgcgcg gctacgctat cctgcgcaac tgccgtttgc
     9361 tcaccagagc gcaaatcctt cactactgct gtgccgttag tcacttcaga ctcacccagc
     9421 accacagcaa cgcgggcacc ccatttatca gcacgggcaa actgtttctt aaagttgccg
     9481 ccgccgtggt tggtcatcaa tttcacgccc tgtaattcat cacgcagacg ctcagctaat
     9541 gccatagccg cagattgtgt atcagcacct gaagccacca ggtatatatc gacaatagga
     9601 tcggctttaa attccggatt aacggcctgt actaacaata caagacgttc gaggcccatc
     9661 gcaaaaccga cagccggtgt tgcacgaccg cccagttgtt ccacaagacc gtcataacga
     9721 ccgcctgcac acacggtgcc ctgggagccg agactgttag tcacccactc gaaaacggta
     9781 cggttatagt aatccagacc acgcaccaga cgctggttta cggtgtaagc gatccccgcg
     9841 ctctcaagca gtttgcacag accggcaaaa tgctcacgag attcctcgtc cagataatca
     9901 cctaatgccg gagcgtcgtt gagaagcgcc tgcacttccg gattttttga atccagcacg
     9961 cgcagcgggt tagtgtacat gcggcgtttg cagtcttcgt ccagcttttc tttatgctgc
    10021 tcaaggaatg ccaccagcgc atcgcggtaa ttggcgcgtg cttccagcga accgatagaa
    10081 ttcagctcaa gagttacgtg ttcggaaata cccagcgcgc gccaccagcg ggcggtgagc
    10141 ataatcagtt cagcgtcgat atccggacct tgcagaccga aaacttcgca gcccaactga
    10201 tggaactgac gataacgccc tttctgcgga cgctcgtgac ggaacatcgg cccgatatac
    10261 cacagacgct gttcctgatt gtacagaaga ccatgctcga tgccggcgcg tacacagccc
    10321 gccgtccctt cagggcgcag agtcaggctg tcgccattgc gatcctcaaa ggtgtacatc
    10381 tctttttcaa ccacgtcggt gacttcacca atcgcacgtt tgaatagcgg ggtctgctct
    10441 acaatcggca agcggatttc actgtaaccg tagctgccga gcacgttttt cagtgtgcct
    10501 tcaatgcgct gccagatggc cgtttcgcca ggcaggtaat cgttcatgcc gcgaatggct
    10561 tgaatgtttt ttgccacgtt tattctcttt ctgaatataa aaatgaaccc tcaacgcttc
    10621 cctcaatgtt tggggagcca tgcgggttca atcatacacg ggaagcgagg cgcttcccat
    10681 cacgttatta tttttcaacc tgctgaacgt caattcgacg cgcttcatcc agctgactgg
    10741 ctttcgcacg aatgcgtgct tccagctggt cgatcatatc gttgttgtcc agacggtctt
    10801 tgcgcacgcc atcttcatag aggccgcttt tcttgttgcc gccggtgacg ccgagtgtag
    10861 aaaccagcgc ctcacctggg ccattcacca cgcagccgat aatcgaaacg tccatcggag
    10921 tgatgatatc ttccaggcgt tgctccagcg cgttaaccgt accgataaca tcaaattcct
    10981 gacgcgaaca ggtcgggcag gcgatgaagt tgatccctcg cgaacggata cgcagcgatt
    11041 tcaaaatatc gaaaccgact ttgatctctt cgaccggatc ggccgccagc gatacgcgca
    11101 gcgtgtcgcc gatgccttca gacagcagca gacctaaacc aatggcggat tttactgccc
    11161 cgctgcgtgc accaccggct tcggtgatcc ccagatgcag cggctgatcg atctgttttg
    11221 ccagcaaacg ataagactca acagcgagga agacgtcaga cgctttcacg ctgactttga
    11281 actgatcgaa gttcaggcga tcgagatgat caacatgacg catggcggat tccagcaacg
    11341 cctgcggcgt cggttcgcca tacttttctt gcagatcttt ttccagcgat ccggcgttaa
    11401 cgccaatacg gatcggaatg tttttatcgc gcgcacagtc aaccaccatg cgaatacgct
    11461 cttcattacc gatattgcca gggttaatac gcagacaatc gacgccgtat tccgctactt
    11521 tcagcgcaat gcgatagtcg aagtggatgt cagccaccag cggcacgtta acccgctgtt
    11581 tgatgagttt gaacgcttct gccgcgtcca tcgtcggtac ggagacacgg acgatatcag
    11641 cgccaacgcg ttccagcgcc ttgatttgat tgaccgttgc ttcgacatct gtcgtacgcg
    11701 tattggtcat ggactgtacg gcgatgggag caccatcgcc aatcggcaca tacccaacgt
    11761 aaatacgtgt tgattttcta cgttgaattg gagcctggtt atgcatgaaa aatctcccgc
    11821 gttacccgtc tgttactgcg ccggtgattg ttcggcattg agggtcagac gcgcaacctg
    11881 gttagttctg ataaaacgac tcagatcgac aggtttccct tgatactgga tctgtactgc
    11941 ggctggcgca ccaattttca gtttgtacgg tgcctggccg gttaagttca aattaccgtc
    12001 tttacgctgc ataccgctaa acaatttttt accggtagca tcagtgacct ccagccagca
    12061 atcggcagta aagttcatca ccagcgcatt cggatcagcc accggcgtgg tcacgccagc
    12121 ctgatcggtt ggcaacggcg cagcaccatc tggcgttgtt gctgccgttg gtgccggggt
    12181 cgcggcggta tcaacatttg cctgcgaagg cgaaacaacc gcattctgtt gcggatcaac
    12241 agctggtgct ggcgcagtta cggcaggtgt ttgcgtgttg gttgcggtag tatccacaga
    12301 cgctggcggc gtggacgttg tagccgggtc tgtagttgtc gacgtattta acggaacact
    12361 ctgcccctgc tcgctattac tgctcagttc cgccgaagat tgatcggcca tagtggtgat
    12421 ctcttcctgc tgagctttgt ggtcttgcca ccaccaggca ccgctcaggc cgataaccac
    12481 aaacaacacc agccaggtga aggtcatcag ccagccgtca cgttttttgc ggcgtttacc
    12541 gagggaaaaa ctctgcatcg gcgcaacttt cgctgcccgt agtggagcct gcttttccag
    12601 ccctggcagc agttcttctt ctggaatatg taccagacgc gcataagagc ggatatatcc
    12661 gcgcaggaat gttgaagcaa gatcggcggg tgccttatct tcttcaatgt cgcgtaccgt
    12721 ggaaaccttc aggcaaagtc gctcggcaac ggcctgctga ctaagtccta gttgttcgcg
    12781 agcattacgc aggcgagcgc cggtagtaag tgcttcattt tggtcgtgcg tggcttcagt
    12841 attcattcgc tgcaggtacg tttaaatgag aattgagatg ccggtgaacc atcatgctca
    12901 cccacaccgc gaaacatccg ttaagttaac cgttatcata cagtataaga ccgtctgtgt
    12961 gctcgtgaca aagcccacat tcactaaggc taacttactg ttgcatcgtt acatactgcc
    13021 ttaaagtcag caaaaacgca ccgttaatat tgaccagaca attgcaactt aatacttcat
    13081 tcataaaaga ttacgccacg gtacataaag taaccgtggc gtaatggcta tcagaccgct
    13141 ttaatgtcga tggcttcacc ctgcatccgt ttacgcaggg tacgtttcgt acggtcgata
    13201 acatcgcccg ccaactgacc acaggcagca tcgatatcat caccacgagt tttacgcaca
    13261 atagtggtga aaccgtagct catcagcact tttgagaaac ggtcgatacg gctgttcgaa
    13321 ctgcgtccat acggcgcgcc cgggaacggg ttccacggga tcaggttgat cttacacggc
    13381 gtatctttca gcagctccgc cagttggtgc gcgtgttcag tgccgtcgtt aacgtggtca
    13441 agcatcacgt attcaatagt gactcggccc tgattggcgt tggatttctc cagataacgg
    13501 cgcaccgcag caaggaacgt ttcgatattg tactttttgt tgatcggcac aatttcgtca
    13561 cgaatttcgt cgttcggcgc gtgcagggaa attgccagtg caacgtcgat catatcgccc
    13621 agtttatcca gcgccggaac tacaccggaa gtagaaagcg tgacgcgacg tttagacagg
    13681 ccaaaaccga aatcatcaag catgatttcc atcgccggaa cgacgttgtt caggttgagc
    13741 agcggctcgc ccatgcccat catcactacg ttagtgattg ggcgctgacc ggtgactttt
    13801 gctgcgccga cgattttcgc cgcacgccac acctggccga taatttccga cacccgcagg
    13861 ttgcggttaa agccctgctg ggcggtggaa cagaatttac actccagcgc acaccccacc
    13921 tgagaagaga cgcagagcgt ggcacggtcg tcttccggga tatacaccgt ttcgacgcgc
    13981 tgatcgccaa cggcgatcgc ccatttaatg gtgccgtcgg atgaacgctg ttcttcaacc
    14041 acttccggtg cgcggatttc cgccacctct ttcagtttgc cgcgcaacac tttgttgatg
    14101 tcggtcatct catcaaagtt gtcgcagcaa tagtgataca tccacttcat cacctgatcg
    14161 gcgcggaagg gtttttcacc taaatcttta aaaaactccc gcatctgctg acggttgaga
    14221 tccagcaggt tgatttttcc atctttcgtg gtgacgtttt caggtgtgac taattgttca
    14281 gacatatgct attccggcct cgttattaca cgttatggcc cctggagggt tgaaaaaaga
    14341 aacgccccgg tgagcggctg ctcgtccggg ggcgctgcat tgtacaaatt ctggcgcacg
    14401 gatgccacgt ttgcacgcgg catttacgaa attattaacg ggtgcgcggg cacacttcgc
    14461 cttcgccaaa gaaataagcg atttcgcgag cggcagattc gacggaatca gaaccgtggg
    14521 taccgttttc ggtcaggctg tcagcgtaat cagcgcgcag agtaccagcc agtgcgtttg
    14581 ccggattggt cgcgcccagc agatcgcggt gacgctgaac ggcgttttca ccttccagca
    14641 cggaaaccac gatcgggcca gaggtcatga attcaaccag accatcaaag aacggttttc
    14701 catcgtgttc agcataaaag ccacgtgcct gttcaacggt caggtgcagc attttggtgc
    14761 cgacaatttt gaaccctgca gcttcaaagc gcgcaaagat attaccaatg acgttttttg
    14821 ctaccgcgtt cggtttgatg atggaaaaag tacgttcaat agccattttt acctctgtaa
    14881 attgttctgt tgttgtctgt accagcgtac gagatggcgc ggattataat gagcaacagg
    14941 gccgttgact attgatgaag gtaacatttt tttaaaataa aatgagtttt agcaacaatc
    15001 agggacaaaa cagactattg catgacaaat ttcactgtcg cgatttgccc cacgtcatcc
    15061 atcaccagca attgataatc acctttatcc gtcaaatgca gggtcacgtt gcgcccgcgt
    15121 tcagttaacg gttcgccatt cagaaaccac cagcgttcac ctgcccctcc acttgattgc
    15181 aacggcaaag tcgcttccgc tgcgcccggt aaacgtttaa taatcgcgcc atcgcgaacg
    15241 cctgtcagct gcagcgggag ctgggcatcg tgaccgtatg gcgggcaact tgtcgaggcc
    15301 ggtggtaagc gcacagcgcg actttctgat gcgggcagcc agggttccag cggtagcggc
    15361 cagacattta tcatttcttg tcgcgcttgc gggcaatcag cggcaacacg tttgccattt
    15421 tcatccagcc agatggggaa acgaatgcca ttaatgcctt cctgctccgg cagtaataga
    15481 gttggcggct gactcccgtc cagcagccag gttgccaggc ggcggcgaca gttaccgtca
    15541 ccttccggca aagactgtcc gcccggccag cagataacgc cacgagtgac tgagttcggg
    15601 cgcgggtctt ccggcagatt cgcactgcgc gacagtaaga tattattgac ctgattcaac
    15661 aatggtacgg cactggcaaa gccaaactga tcaacaacgg gcgtgccgtc cggtctgcca
    15721 gtccagatcc caatgacata gcgagcgtta accccaatcg cccaggcgtc acgatagcca
    15781 tagctggtgc ccgttttcca tgccagtggg gcgacgcgcg gcaaggcact atccggcaag
    15841 ggttgcgctt catcagccat aatccggcga atgatccacg ccgcccccga cgacattaaa
    15901 ggccgttcaa gcagcggatc gtcaggctgt aagcgcaatt tgcctgcctt gccgtggcga
    15961 gcaaacgcgg tatacgctgc cgccatatct tccagttttg caccagcgcc gccgagaatg
    16021 agtgaaagat tcggcgcagc accgttgggc aaatataacg gcaatccaac attgcgtaac
    16081 tttgccgcaa accgtttcgg tccataggct tccagcacct gcacagcagg taagttcagc
    16141 gagcgcacca gcgcctcgct catgctgatc gggccatgaa aaccgctatc aaagttacct
    16201 ggtcgataat caccggtgcg ccgggggacg tcttgcagca gtgatgccgg gtggatcaag
    16261 ccttcatcca gcgccagacc ataaacaaac ggtttgagca ccgatcctgg cgatcggatc
    16321 gcattgacca tatcaacatg accaaagcgt gaatcatcgt tgagatcaac cgatcccacc
    16381 cagccgcgaa cacgcatatc ggtatgatca accacgatca tcgccagtga gctgcgcggt
    16441 ggcaaccgcc ctttccagtt ttgcgccagt tcttccagac gtcgttgaag accggcatcc
    16501 agcgtagtaa cgattttgtc gcttttgctt ttaccgagca tcatgcgcga aaacagcggt
    16561 gccagttgcg gcatttgtcg gggtgccagc cagatgggtt cttcccttga ctcttttacc
    16621 tgctcacggg accacacacc ttgcacggcc atccgttcga gcactttatt acgcgcggct
    16681 tcggcacgct ccggccaacg atccgggcgc agtcggctgg gcgcttgcgg taacaccgcc
    16741 agcattgccg cctcggaata gcttaaattc gcaggcgatt ttccgagata agcccaactt
    16801 gccgcaccga tcccctgcaa cgtaccgcca aacggagcgc ggttaagata caagatcaga
    16861 atttcacgct tagacaaatg ccactccagt tgcaacgcgc gccagagctg gagaattttg
    16921 ccaccaaatg ttttggggtg aggatcaagc agacgagcaa cctgcatagt gagcgtgctg
    16981 ccaccagaaa taacccgtcc cgaagtgaga tcttgccatg ctgcgcgcgc caccgagaat
    17041 ggattcaccc ccggatgctt ccagaaccag cgatcttcat aattgatcag cgcttcaagg
    17101 taacgtggag acacatcttc gattgttacc ggataacgcc agatgccatc agcatcggcg
    17161 aagcgccaga gcggcgtacc atcctgcgcc acgaccactc gtgcgggatt gacttcatgc
    17221 agcggtagag gccagagttt atctgctccc cacgctgcaa gaaaaaggag aaatggcaca
    17281 gctgccagcg ttatccagca gccgcgtttg gttaacaagc gaagcgttta cggtcggaca
    17341 atcagcagat cttcagccgc gccggtcgcc cgccattggg gaacatacat tgattccacc
    17401 atcggttgcg gtacctgata cgttcccggc gtcaccgccc gcgccagata caccagcgtt
    17461 accggttggt attcatcaac ggcaaccgcc gccacaaagc gatcgtcacg gaactcaatg
    17521 tgctaatgct cgcctgctgc atctggttca gtaagttttg cacttcgcca ccactttgct
    17581 ccaggctggc gctaccgttc gccagattct gattttccag ttccagaccc gcaggcagca
    17641 gatccacgac taacgcatcc ggcacgctgt tactggcttt tacctgcaac cagaccagca
    17701 ccagatcgcc gctacgtaac gagtccagcg atttgctctt accatcagta ccaagaatat
    17761 gacgctcgat ttgcagcaca ttgttcgccg gtaaaggtgc ggattgcgga taaccgctgg
    17821 catccatacg caaccataac ggctgatcgc cactgtttct cacctgcaag gtggcaagtt
    17881 gatcgctatt cagattgctg tttagcgttt tctcgcctgt cagcggctca gctgagaaag
    17941 aggtttgcgc ctgccattta cccggtaaat cctgaatcgt acgggcagcc aggaacaacg
    18001 cgttactttc ctgcgtcgat agccagcgtt caccaaacgc ctgctgcgaa agtgtgttca
    18061 gcaaagtgta ctgctcatcg ggtagcagtt tattttcttc cagcaaggag agcattaacg
    18121 cgttgtcgcg cagtggacta ccgtaatcac ccagccatat ccgctcatca ctatttctcg
    18181 gcgttttcag cgccagcaca atcgcttctt caccacgcgt tgcatcaccc atggttttca
    18241 gcgcaatgcc aagttgcagc agcggtaaac cagaagcggc atctgcacga tgctcccaga
    18301 tttcacgcag cgcacccagt ggtgcttttt gctgacgggc cagcaccagc gcagcgtaag
    18361 actgaacggc gaatttactg gctttgagat tatccgcgta cgggatcgac atcatgcccg
    18421 gatcttgtaa atagcgcagc agacgctcat taccccggtt aatggcgtct gtcggcacgc
    18481 tgtaaccctg ttcgcctgcg cggaccagga aatccatcac gtaagccgtc agccagtact
    18541 cttcgtcacc gtttttatcc cacagcgcaa agccgccgtt atcacgttgc atttgcagca
    18601 aacgggaaat gccgatatcg accgatgcac ggcgtttctc atcactgtcg cctttgatgc
    18661 ccaacgcctg cagttgggct gcgttggtat aaagtgacgg gaacaggccg ctggcggttt
    18721 gctcaagaca gccgtacgga tacgctttta actctttgat ataacgtgca atgttcagcg
    18781 gtggtttgcc gctcaacaac aattgccctt ccagcgtaac aggcgagaag ttttgcaatc
    18841 catccgccgg aatcgcccat gtctcaccaa gctgtaacgc cgtaccgtaa ttaaccgttt
    18901 gtgccgggaa cgccggacgg acgccgattt tccactgctt atgctgatcg gcaacggttt
    18961 cacccggtaa cgctaacccg ctaatggtgg cctgaatttc tccatcgcca taacccggca
    19021 atgctcgcac cgggataaac agcgtagtac gcacacctgg cgctaattca acggctgcgg
    19081 gtgaatcgct gaccagttca agcaaaccac tggcggtcag ggcaacgttc agtttttgcg
    19141 gtttatcggt aagattagtg atatccagcg tcagacgcga ggtatcgcca ctcgccataa
    19201 agcgcggcat gttcagttca gcaatcaccg gtgcggcaac tatcactttg ctttcgttgc
    19261 taccgaaatc atctgccgtc caggcttgcg ccatgacgcg caattcaccg ttaaaatcgc
    19321 caatcggcag tgtaaccgag ccttcgccct gttcgttgag cgttaccggc agcgcctgct
    19381 gcgcgacaat attgacgtga ttgaccggcg gtttaccacc acgtttcagc tcatcaccat
    19441 cgccaccgaa acgcagagct gccagacgcc cctgaccttc aataacctga ccgtaaatat
    19501 cgtaaatgtc tgcgccattg cgtttctgac caaagaacgc ctgccacgga tctggcgtga
    19561 cgtagtcagt aatattcagc acaccactat caacggcaga caccagcaca ttcacctgtt
    19621 taggcttctc gccattttta gtgctggctt taattttcac ggttaatggt tgattgggac
    19681 gcatttttgc tggtgtttcc agcgccagat cgaggcgacg gttttcatcg ccaagcggca
    19741 gatgcaacac accaaccgcg cgttttggcg tcgcggagcg agatttatcg ccaggacgta
    19801 ccaccagcgt acttaaatac agatcatgac gattccaggt tttatcgacc ggaatcgtca
    19861 gatccagccc ttgagccgga acatcaatct cttgccacca cagcggccct tcactggact
    19921 cgaccatcgc ataaccttta cccgccgttg gcgcggcgat atgcaactta atggtgtcgc
    19981 cagggcgata actggcttta tccagtttca gcgtgacacg gtcgggtcgc actgcgccgc
    20041 taccgtcgct gttgtcctgc cagctatagc cagcccagaa acgaacacta ctgaccgctt
    20101 cattcggcgc tttgacttcc agacgataag cgccccactc taccggaaaa ctgaccttgc
    20161 cggtttcgtc cgctttcaga tccagagttt gttcattttc gatcagatct ttttgatcaa
    20221 actgtgactg ccagccttca tcttctgacc agttccagta gtaatcgcgg cgttcgcgaa
    20281 tcaggcgcac ctgcaagccc gacacggctt ttttcacgcc ttgtgcatcg ctataaacga
    20341 tgtcaaaagc ggcgttactg ccttcatcaa caatgggctg tttcaccgtg ctgtcagtgc
    20401 gataatcgta aaccgatttc aaggcgaact gcggacggag ccccggcaat gcatcggcag
    20461 gccagatagc ctgctcagcg cggcgcgtca ccgggcgacc gcccgattcc agcaaactgc
    20521 cctggaaaat aacctgcaat ggggaatgtg tttccttcca ctggctttct gtagaaactt
    20581 cgccgcgccc tttatcatcc agcgtcaact gaacttcatc cagcgtgcgg gaaaggtttt
    20641 cggcagctat atcgccgaat tcaaaaccag gtaaggctga cacagcctca cgcagtgggc
    20701 gcaggaaaag ttgcccttgc aaagtattac cattagcagg cgcaccgtac aggtagtatc
    20761 ccaccacgga gaatttcact tcatcattcg gcgtaagcgg ggttttctca ccggtcagat
    20821 tcagcgccat acgctctggc ataaaatctt cgacgtggaa atcccacatc cggtactggt
    20881 tatcgcccgt gttggcgcga atatgccaca taccggttgc cgcattgcta tcgagtggcc
    20941 aggtaaagtg gtagaggcca ttctccggct gactaacgac gctcctgagt acctgcccgt
    21001 caggtttaat cacgtctaac ttgatgggtt gatcgggcaa tgctttaccg tctgcatcgc
    21061 gcagtaaacc attgaggatt accgtttcac ctgggcgata aagatcgcgt gggccaaaca
    21121 tgaaaaactg tttgctatag cctggcgcgc cagcaatgtt aaattctgct aagtccagcg
    21181 ccggaagttt taaatcgagt agcgttgtct gaccgtcttt acgtgccagc agtaatgccg
    21241 catttttatc attttccagc tgcacatgcc cctgagcgtc actggttgcc tgagtcagag
    21301 tctgcccttt ctcatttaat aaagagactt caattccttg ctgggccgcg ccgttttcca
    21361 gactttgggt aaagatatcc agacgattgt gataacggtg agctgaaacg ccgatatcac
    21421 ttaacgtaaa cagcgtcgcg ggattactgt aatcgtaacg tccagcctga ttcatcacag
    21481 ccagatacac gcccgcctgc tgaagcggtt tgatatcgcc cagcggcagc aataattttt
    21541 cacgggtgtt acgcgcagga ttgagatcaa accgtccggt gtagaccaga tccgccatct
    21601 gcagcagttt gtctgactgc cagttcgcca gcgaattgcg gtattcccat tggctaatga
    21661 atgctggcag agattctggc ttaacgcgga agaagttaac atcgacatta ttaacgttga
    21721 gcgccattac cggcagccct tcaacgactt tgccaggcag cagcgaacca cggctggcaa
    21781 aaccgacgct gggttggatg tcgcgggtag ttatagtttt ttcgtaatct ttactgaagg
    21841 ttgcgttgtt gagtgctttg acctccttgc caatagtaac gatcaaatca cgtttcggtt
    21901 cgaggtggcg taaacgtagc tctttaagat tatctgacag ctcccaggca ccatccactt
    21961 tgccgctttt tttatcgacg acatgaataa cgcgtgagaa atcctgatcc gggtcgagag
    22021 ggatggagaa cgtcagcacc agcgtggctg caccatcaag ttggacttca gagagatcca
    22081 gcaatgtcag caccttaccg gcactctgtt gggccagttt ttgtctctcc ggtacggaga
    22141 gttttgctga actcgcgttt tctgaagagg ccgctttagt aacttcagaa ggcgcatctt
    22201 ttttcaccgc tgttggcgcg ttatcgttgt tgtcgcaccc tgccagcgcc agcattagca
    22261 tgcaggcggc tacgcgtaac tttttcattt ttcatccctg ccacaatggc ccgttagcaa
    22321 cgtcgaataa ttattatgcg tgagaattta cgttatgtaa ttcagtatca cgcccataca
    22381 atccgaattt tagtgagaat tgtggctcag acagccaaat cagcctcttt cgcttgtcgc
    22441 ttttcactaa acatccgaca atttagccta accccggcaa aaatggagat gcctatgtcc
    22501 acgacatggt ttgtaggagc cgactggctc gccgaacata ttgatgaccc ggaaattcag
    22561 attatcgatg cccgcatggc gtcgcctgga caggaagatc gtaacgttgc tcaggagtat
    22621 ctgaatggac atattcccgg cgcagtgttt tttgatatcg aagcgctttc tgatcacact
    22681 tccccgcttc cgcacatgct gccgcgcccg gaaacgttcg ccgtggcgat gcgtgaatta
    22741 ggcgttaacc aggataagca cctgattgtc tatgacgaag gtaatctttt ctcagcccca
    22801 cgagcatggt ggatgctgcg cacctttggt gtagagaaag tgtcgattct ggggggtgga
    22861 cttgcaggct ggcagcgcga tgatctgctg ttagaagaag gtgcagtaga gctgccggaa
    22921 ggagagttta acgccgcgtt taatcctgaa gccgtggtga aagtaaccga tgtattattg
    22981 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
    23041 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggagc actgaatgtt
    23101 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
    23161 ttttttggtc gcggcgtcag ctacgacaaa ccaattatcg tcagctgcgg ctctggtgta
    23221 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
    23281 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
    23341 gtattttaca ggcaataaaa aaccgccgaa tttggcggtt ttttattgct ggtctggttc
    23401 gcggcctttc cagcaggttg tattaccgta gtaatgcaag cgcgtctcag cggagacaat
    23461 actcgccagt aactctcttt ttgtcaagca aaagagagtt attattgttc tgttagtata
    23521 ttatccactg cggccctttc cgccgtctcg caagcgggcg ctggcttaag gaaaggatgt
    23581 tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat cgcggcaata tcgccagtgg
    23641 tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa gatacggtgc ttctgtatcg
    23701 tacttattgt ttctggtgcg ctgttttccc aagtaaataa taaccggagt ctctccggcg
    23761 acaatttact ggtggttaac aaccttcaga gcagcaagta agcccgaatg ccgcccttta
    23821 gggcggcata ttttagatta tccggttttg tttaaagtca cgcaaaaaac ctccccagcg
    23881 acgttcatag aatggcacaa tatgttcggt aataaagtgg ctaattcctt tttccccttt
    23941 tttcacctga caaatatcga ttggttcatc gccaggtaat gtatcggtcg ctacacttcc
    24001 cgtcgcctga ataatttctt cgatatcacc atcggcttca atgccaataa ataaattagg
    24061 ctgtgcctct tcgttctctt taattgaaca aataaaagca cgcttcaccg gcttaatggt
    24121 tttaaataag gtggtgagtg aatcaatcat ttgtgctggc ggctctgcga cttccgataa
    24181 tatcagcgat tcaccgcctt ccaggatttc ctggctgctc agcggatttc cctcttcacc
    24241 aatcaacaaa ctgatttcac gcggcataaa ttctttaccg gtcggcagtt tggcattaag
    24301 gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga acgggcatta cgacaaatgc
    24361 ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag gtgaaaaaag gaatgacgct
    24421 ggtgccgtct tctttttccc agtgctgtaa atcaagcgca ctatcttcaa ccacagcctc
    24481 gccctgcgcc gccgtaccag gcacccagac ggtggattcc agtagagtac ggaaaaaggc
    24541 cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt
    24601 ttcggacata agaaccacaa ttcattcaac gttcggggcg caaatgcctg atgcgctacg
    24661 cttatcaggc ctacaaggga ttcgcaattt gttgaatttg cagaatttgt aggccggata
    24721 aggcgtttac gccgcatccg gcatattagt ttacgccgtt aacagattag ctatcgtgcg
    24781 cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc
    24841 cgagcagtcg atatgcagcc agccttgctg atagttctca acaaagtgcg acaggaagcc
    24901 cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg gtattgttca gttcggcaaa
    24961 gttagacggc agctggctgc ggtggaattc cgccagcggc agacgccaga atggttcgtt
    25021 ctcttgtgag gcactcgcca gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc
    25081 gtgataatca ttacccagcg cagttttcgc cgccccggtg agggtcgccg catcaatgat
    25141 catttccggt ttctgcgcac tggcatcaat cagaccatcg gcaagcacca gacgcccttc
    25201 ggcatcagtg ttcatcactt caactttttt accgttgcga taggtgatga tatcgcccag
    25261 cttgaacgca ttgccgctaa tcaggttatc cgcacagcag aggaacagct tcacgcgctt
    25321 gttcagtccg cgcgtaatgg caaatgccag cgccccggta accgttgccg cgccgcccat
    25381 gtccgacttc atcgagtcca taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa
    25441 agtgatacct ttacctacca ggcacgcgta cactggcgct tctttatcgc cagttgggtt
    25501 gtaatccagc gccagcaata ccggagaacg ttctgaagcg cgtccaacgg tgtgcagccc
    25561 catataacct tgctcacgca gatcttcgcc tttggtgatc cgataagtca cacgatcgcc
    25621 cgcgacgttg ctgatcagat caacagcacg ctgtgccagt tgcgatggtc ccaattcttc
    25681 tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc atcaggcggt tatccagttc
    25741 ctggcgctgg gcatcgtcca gatccggcca ctccacttta cgcgtgcctt tcggggcttt
    25801 gtaaccttgc cagaatgccc agcagcgatc cgcatcccag ccttcaccgc ttaactgaac
    25861 atgcttgatg cccagaccgt caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc
    25921 tgccccgttc aaatgcaggg taatgccgtc attattaatg ctgtaagttg ctttttctcc
    25981 ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc atcgcttctg tcattttagt
    26041 tatccttctt tgtaaaaggg ccgccagttg gcagccccag gatttttccg ctaaatgatt
    26101 cgcgttgcag gaaggcggca agtgagtgaa gccccaggag catagataac tatgtgactg
    26161 gggtgaacga gcgcagccaa cgcatctgcg gcgtgaagca tgacgcggaa attattcggc
    26221 ctcgtccagc cagactaaca aaatcgcttc gaggattttc tcgttggatg cctgcgggtc
    26281 gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt
    26341 cggatcaaga tcaggatacg catcgtacag tgcttcgcca atttcgcggc tatcggtcca
    26401 cttaagtccc atactaacct ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg
    26461 atttcgacta ctaaatcttc gtcggtaacg cgtgcctggc agcttaaacg gctttcgggc
    26521 tccagtcccc aggctttgtc cagcatgtcg tcttcctgct ctgagctttc tggcagcgag
    26581 tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag cacaggattt ttcacaggcg
    26641 tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa tggtttcacc gctattagct
    26701 tccagaacag cgccatcagg gcagaggtcc tgatgaggca aaataacaat ctttggcata
    26761 ttaaacctcg tccacggaat ggcctttcag cgcacgacga accgactggt ccatgcggcg
    26821 agcggcgaaa tcctgggttt gtttgtctac gtttttaatc gcttgttcga tggcgtcaac
    26881 atcagcgccc tgagccactt cactcaggtg agcggcagca ttgtcaatga cctgacgctc
    26941 tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg tgcagacttt ccagcacacg
    27001 cgccgcttct actttttgtt ctgccagcat acgggctttt acgtcctgct cggcatagct
    27061 cattgaatct ttgatcatcg aagcgatttc gctatcggtc agaccgtaag aaggtttgac
    27121 ctgaatagac gcctcaacgc cggtggattt ctccatcgcc gtcacgctca aaagaccgtc
    27181 ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca gccggtagcg ccggaatacc
    27241 acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc agttcgcgct caccctgcat
    27301 tacatggata gacatcgccg tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc
    27361 caccggaata gtggtattac gcggaatcac tttctccacc aggccgccca tcgtttcaag
    27421 gcccagcgac agtgggatca catcaagcag cagcatttcg ctgtccggct tgttacccac
    27481 cagaatatcc gcctgaatcg ccgcgccaat agcgacgact ttatccgggt cgatggaagt
    27541 cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc accagcggca cgcgagtaga
    27601 accgccaacc atcaccactt ccagcacttc atcagcttct acgcccgcgt ctttcagcgc
    27661 acgacgacaa gccagtaagg ttcgttttac cagtggcgcg atcagttcat tgaattgttc
    27721 acggctgatt tcgccctgcc agcccgcaac gttaacggtc acggagtccg catcgctcag
    27781 cgcgattttg gctgcaatgg cggcatccag cagttcacgc tgaacgcggt tatcgctacg
    27841 atcaggaatg tccgcctgct cgcgaatgta atccgccagc agatggtcga aatcatcgcc
    27901 gccgagcgcg gaatcaccgc cggttgccag cacttcaaac acgccgcgac ttaagcgcag
    27961 aatggaaata tcaaacgtcc cgccaccgag gtcataaacg gcgatcacgc cttcctgacc
    28021 ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg
    28081 caaccccgcc agacgcgccg cgtctttggt gccctgacgc tgggcatcgt caaagtacgc
    28141 cggaacggtg ataacgacac catccagctc gcctgccagg gcttcagttg cccgcgccgc
    28201 cagtgctttg aggatgtccg cagaaacgcg caccgggttc agcagccccg ccgccgtttc
    28261 aatcattggc aggccgtttt cgctggcctg gaattgataa ggcaaatgcg gatagcgttg
    28321 ctggatatca gccagcgagc gtcccatcag gcgtttaaca gaactaatgg tgttggcggt
    28381 atcgagcgct gcgttagtac gcgcgtcata acccaccgaa tgcccttgct gttgatagtg
    28441 aacaacagat ggcagcaggt gacggccttc atggtcggct aacgtttcgg cctgaccgct
    28501 gcgcactgtc gctaccagcg agttggttgt gcccaggtca ataccggccg ccagacgacg
    28561 ctgatgcggc gcagcactca aaccaggttc actaatttgt aataaggcca tgtttagctt
    28621 ccagaaatta aaaatcgagc agtttttctt cgagttgttc ggcactgctt cgcagtttat
    28681 cgagaaaacg cagcttacgc acggtatccg ccgccgcgtc ccacgtctcg ttgtctaact
    28741 gttcaaccat caactgatgg cgggtatcaa acatcttttt cacacgtttg ataaagcttt
    28801 ccagccgcgc ttcatctttc gcctgttcga tctcgtccag ttcttcgcgc agctccagct
    28861 gttccatcag gaacggggtg tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt
    28921 gcaaagaaag caaatattcc gcgcgcatta acggatgacg cagcgtttgc caggcctggt
    28981 taatggttgc agattgctgt acggcggcga gttgttccgc ctggcttccg ctggcgaatt
    29041 tatcaggatg atactgacgt tgtagatcct gaaaacgcag gctcagtgcc tgggtatcga
    29101 gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc cataacattc tcagggctgc
    29161 gatccgccgc gataacggcg aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg
    29221 gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg caaccacact catctttaac
    29281 gttcgggttg gtgaatttaa acccttcatt caggccttct tttacgaagt ccagctgcgt
    29341 accgtccaga aattgcaggc ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa
    29401 cacgatgtct tccggcgtcg gttcgtcaac aaattccagt acataagcca tacctgaaca
    29461 tccggaggtt ctcacgccca gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt
    29521 atttactcgc gctgctgcac tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt
    29581 attttgcttc acgtttgctt ttatagtccg caatggcggc tttgatcgcg tcttctgcca
    29641 gaatagaaca gtgaattttc accggcggca gttcaagttc ttcagcaata tcggtgtttt
    29701 tgatcgcctg cgcttcgacg agagacttcc ctttcaccca ttcggtgacc agggagctgg
    29761 aagcgatagc agaaccgcag ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat
    29821 cgttgacttt aatctgcaac ttcatcacgt cgccacaggc cggtgcaccc accatgccgc
    29881 tgccgacgtt ctcgtcgttg ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt
    29941 cgataacttt ttcgctgtaa gccattataa attctcctga ttccgatacc gattaatgat
    30001 gagcccattc gatgctgttc agatccacgc cctgcttgta catttcccac agcggagaaa
    30061 ggtcacgcag acgaccgatg gatttacgaa ctaactcgat ggtgtagtcg atctcttctt
    30121 cagtagtaaa acgacctaaa gagaaacgga tagagctatg tgccagctcg tcgttcagcc
    30181 ccagcgcgcg cagcacgtag gacggttcga ggcttgctga cgtacaggcg gaacctgaag
    30241 aaactgcgag gtctttcagc gccataatca gcgactcacc ttcaacgtag ttgaagctga
    30301 cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat
    30361 ctttgatgcc gttccacaga cggttacgca ggccgcgcag acgttccatc tcggtcgcca
    30421 tctcttcttt tgcgatgcga taggcctcgc ccattccgac gatctggtga acaggcagag
    30481 tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc
    30541 gcggtttacg acgtacatac agcgcaccga tacctttcgg gccatagatt ttgtgaccgg
    30601 agaaagacat caggtcaact ttcaactggc tcaggtcgat aggcagtttg cccacgctct
    30661 gggttgcatc aacgtgatag ataatgccac gagcacggca catttcgccg atagccgcga
    30721 tatcctgcac cacgccgatt tcgttattta cgtgcatgat ggaaacaagg atggtgtcgt
    30781 cacgcatcgc tgcttcaagt tctttcaggt cgataatgcc gttacgctgc ggtgccaggt
    30841 aggtgacttc aaaaccttcg cgctccagct gacggcaggt atccagtacc gctttgtgtt
    30901 cggttttgct agtgatgatg tgcttgcctt ttttctgata aaagttggct gcacctttga
    30961 tcgccaggtt gtcagattcg gttgcaccag aggtaaagac gatttcacgc ggatcagcgc
    31021 cgaccagatc ggcaatctga ttacgggcga tatctaccgc ttcttcagcc tgccagccga
    31081 aacggtgaga acgagaggcc gggttaccaa aggttccgtc catcgtcatg aactgcatca
    31141 ttttctcggc aacacgcggg tccaccggcg tggttgcgga gtagtcgaga taaatcggta
    31201 atttcattgc tctataaact ccgtacatca ctcaatgcaa ggaatcaggc tagcggctgg
    31261 atgtacgacc gtgtttacgg agtatttagc actccggcct gattctgaat tctttttatt
    31321 aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat
    31381 gctgacgacc agacacatcc agcacttcct ggttattaac cagttcgcct aaagtaatgt
    31441 tgttgagaaa accggtgaga cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt
    31501 tatcgccgcc ctggcagccg cctttaccct gacaacgggt ggcatctaca gattcgtcaa
    31561 cggcgctaat cacttcgcca acggcgatgc tgctggcatc tttgcctaac agataaccac
    31621 cgcctggtcc acgtacgctg gaaaccagac catttttacg cagacgggaa aacagttgtt
    31681 ccagataaga aagggaaatt ccctgacgtt cggaaatatc agccaacggt accgggcccg
    31741 cttcagagtt gagcgcaacg tcaagcattg cggtcacggc atagcgccct ttagatgtca
    31801 gtctcatgtc ttacttcacc tcaaactcgc ccctgcccag ggttttttat tgtaaaagtg
    31861 ggggtattga atagcagggc caagtctgac attcccgagt aaattggtca actatttact
    31921 tgactgattt agtcgggtat ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc
    31981 tgttgtcgga tgcggtgtga acaccttatc cgacctacgg ttctgtacat tgtaggcctg
    32041 ataagacgcg ttaagcgtcg catcaggcaa ctactgttat tcggccttgt tacctttatt
    32101 ctgctgctca atagaagcca gaatcccgcg caggatattc aattcctggc tttccgggcg
    32161 cgcacgggta aacagacggc gcaatttatt catcacctgc cccggatggt tttcacggat
    32221 aaaaccggtt gccagcagcg tttgttccag atgaccataa aaacgctcca gatcatcgac
    32281 cagcggatac ggcgtctctt catgctcgac ctgctcgccg ttttcctgag ttgccagcca
    32341 ggccatgcgc acttcatagg caataacctg aaccgccatc gccaggttca gcgagctgta
    32401 ttccgggtta gccgcaatcg cgacatgata atggcatttc tgcaactctt cattggtcaa
    32461 gccgacgcgc tcgcgaccaa acaccagcgc caccggggta tttgccgctt cagcgacgct
    32521 tttcaggccg cattcgcgcg ggtcgagcat cggccatggc agcgtgcggg aacgtgcgct
    32581 ggtgcccacc accaagctac aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc
    32641 attaccaatc acatcgctgg cccctgctgc cagggcaatc gcctgggagt cgggtttcac
    32701 cagtggatta accagccaca gattggttaa tcccattgtt ttcatggcac gggcaacaga
    32761 acccatattg ccggtgtgtg acgtctccac cagcacaatt cgaatatttt gcagcattgt
    32821 ctttcttcat ctaaagatta ttcgcgcatc ttatcataaa acgaagacag atgccgatct
    32881 cgctgctata ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga gaccgatgca
    32941 tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa
    33001 ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc agtaacgatt tcgtgaccaa
    33061 cgtagataaa gctgccgaag cggtgattat cgacacgatt cgtaaatctt acccacagca
    33121 caccatcatc accgaagaaa gcggtgaact tgaaggtact gatcaggatg ttcaatgggt
    33181 tatcgatcca ctggatggta ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc
    33241 tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg gtatacgatc ctatgcgtaa
    33301 cgaactgttc accgccactc gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg
    33361 tagcaccgct cgcgatctcg acggtaccat tctggcgacc ggcttcccgt tcaaagcaaa
    33421 acagtacgcc actacctaca tcaacatcgt cggcaaactg ttcaacgaat gtgcagactt
    33481 ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc gctgcgggtc gtgttgacgg
    33541 tttctttgaa atcggtctgc gtccgtggga tttcgcggca ggcgagctgc tggttcgtga
    33601 agcgggcggc atcgtcagcg acttcaccgg tggtcataac tatatgctga ccggtaacat
    33661 cgttgctggt aacccgcgtg ttgtgaaagc catgctggcg aacatgcgtg acgagttaag
    33721 cgacgctctg aagcgttaat gactcaggcg ggtgatgtca ctcacccgcc ctcgcctttc
    33781 aggcgctatt ccgaaatact tcctcaccgc tttactttct ttcgtcactc tcccaccatc
    33841 tttccccgat taatggatga taaagaagtc acaaggaaag gttatggcgc tgccagtgaa
    33901 caaacgcgtt ctcaaaattc tgtttattct ctttgttgtt gccttctgcg tttatttagt
    33961 gccgcgcgtt gccatcaact tcttctatta tcctgacgat aaaatttacg gtcccgaccc
    34021 ctggtcggcg gaatccgtcg aatttacggc taaggacggt actcgtctgc aaggctggtt
    34081 tattccttct tcgacgggcc ctgctgacaa tgccatcgca accatcattc atgctcacgg
    34141 caatgccgga aatatgtccg cccactggct gctggtcagt tggttacccg agcgtaattt
    34201 caacgttttt atgtttgatt atcgcgggtt tggtaaatca aaaggcacgc cgtcccaggc
    34261 cggattgctg gacgatacgc aaagtgccat caatgtggtg cgccatcgta gtgatgtaaa
    34321 cccacaacgg ctggtgctgt tcgggcagag tattggcggg gcgaatattc ttgctgttat
    34381 tggtcaggat gatcgtgaag gcatacgtgc ggtgatcctc gactccacat ttgcctctta
    34441 tgcaaccatc gccaatcaga tgatccccgg cagtggctac ttacttgatg agagttacag
    34501 cggcgaaaat tatatcgcca gcgtcagccc gatcccgctt ttactcattc acggtaaagc
    34561 tgatcacgtt atcccatggc agcacagcga aaagttgtat agcctggcaa aagagacaaa
    34621 acggttgatc ctaatcccgg atggcgaaca cattgatgct ttttccgatc gtcacggcga
    34681 tgtttatcgc gaacagatgg tggactttat ccttagcgcg ttgaatccgc agaactaacc
    34741 catgatcgct agcacgataa tcattcacaa aaccacctta agacatgcta atccactggt
    34801 cagaacagtt taagatgaga aaaattctgt gacgcttgcc aacatttctg atgattagca
    34861 ttcccttcgc catttccctg agcaaacttt agctattctt atcaattatg cttatgggag
    34921 atctacagat gatgcctacg cttgctccac catctgtcct ttcggctccc cagcgccgct
    34981 gccagatctt gctgacgctc tttcagccgg ggttgaccgc caccatggca accttcagcg
    35041 agcttaatgg tgtggatgat gatattgcca gtcttgatat cagcgaaaca ggacgggaga
    35101 tcctgcgcta tcatcaactc acactgacga ctggttatga cggtagctac cgggttgaag
    35161 gtacagtgct taaccaacgt ttgtgtttat ttcactggct acgacgtggt ttccgtctgt
    35221 gtccgtcatt tattaccagc caattcaccc ccgccctgaa gagtgaactg aagcggcgcg
    35281 gaattgcgcg taacttttac gacgatacca atctacaagc gttagtgaat ctctgctccc
    35341 gacggctgca aaaacgcttt gaaacgcgcg atattcattt cctgtgtctg tatctgcaat
    35401 attgtttgcg gcaacaccac gctggaatta cgcctcagtt taatccgctc caacgtcgct
    35461 gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat tggacgccac tggcagcgtc
    35521 gggcgctcca gcctgtaccg cctgatgagc cactgtttat ggcattactt ttttccatgt
    35581 tgcgggttcc cgatccattg cgtgatgcgc atcagcgaga cagacaattg cgtcagtcta
    35641 tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg
    35701 ggttatgcga tcagctttat acccacctcg cccaggcgtt aaatcgcagt ttgtttgcca
    35761 tcggtattga taataccctg ccagaagagt tcgccagact gtacccacgc ctagtgcgca
    35821 ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg
    35881 aaagtggtct ggtcgcggtg attttcggtg cctagctaat gcaggaaaac gatctgcatg
    35941 aaaaacagat tattcttctt accgggaatg atagcgagcg agaagcgcag attgagcagc
    36001 agttacgcga actaacgtta ctgccgctca acattaaaca tatgtcggta aaggcatttt
    36061 tgcagacagg cgctccgcgc ggcgcggcgc ggcgcggcgc ggcactgatt attgcgcctt
    36121 ataccatgcc gttaccgctc ttttcaccac cgctgatcta tacggacctg acactgacaa
    36181 cacatcaaca ggagcagatc cgcaaaatgc tcgaatcagc atgagggaac aactttcggg
    36241 cgcaaaaaca ttgccggaag cgccaccagc gccattaccc agaacacgcc gtggcccaga
    36301 tattgataca ggaaaccggc gaaaacggtc atgatagcta tactgccgcc catcgcgacg
    36361 gcagagtaaa ccgcctgtaa acggatgact tcgctaccct ggcgagcggc aatatagcgc
    36421 atggcggcca ggtggcagac cgtgaaggtg ccgcaatgca gaatttgcac cactatcaac
    36481 cacggcaacg ccgtagttgc tcccataatg ccccagcgca ctacgccgca aatcgccgag
    36541 atcaacagca tatcgcgtgc actacaacgg cggaaaagtt tattactcag cgcaaagata
    36601 atgacttccg ccaccacgcc cagcgaccac aaatacccca ccgccgaggc cgagtagcca
    36661 gctgcctgcc agtaaatggc gctaaaaccg taataggccg catgtgcccc ctgcaataaa
    36721 caaacgcagg ccagaaagcg ccagttctga agaaccagcg ccaaccacgc tgaccaaccg
    36781 gtgctctcct gctggcggct tgccccttgt ggctgaatcg tcggacggat gagaaagccg
    36841 agcagcatgg atgccacgcc caatgttaac agcgcgagga tcacctgata atcaaacata
    36901 gtgaccagtt tgcccgtcag cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc
    36961 actttgccgt aatcaagcgg gaactgcttt tgccacgtat tcgccagtgc atcggtcaac
    37021 ggtaccagcg gtgagaaaaa gaggttaaag ccaatcatca ccagcatcag ccacgctacg
    37081 tgcgcccccg cccagaaggc gacagcaaag agaagtgtca tcattgccag cacgcgcaag
    37141 gcggaaatca ggcgagaagg atcgctgacg cggggcgcga tgagcaaact cccgagaaaa
    37201 cgggcaacca gacctgcccc caataacagg ccgatggttt ctggcgttaa accaatccct
    37261 ttaagccaga cgctccagaa aggtagaaaa atgccataac taaaaaagta tgtgaaatag
    37321 ccgagcgcca accagcgcgt ggattgcaaa accatgagtc cctcccgtca ggaggcgtta
    37381 gtctggggtt aatcgaatgt tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc
    37441 cgatgtctgt aacgccagtt cacaagcact aaaaaactgc gatccgcgac gcatgttgca
    37501 aaaataatgc gtaattatgc cgttatgctt gccaaacgtt cctgcacaat ggcgatgaaa
    37561 tcgcgtaatg ccggcttcat ctcccctttc ttccacgcca tcagtaaagc aatggaaggc
    37621 acatttccgg caatgggacg aaaaacaacc tgtccggtat taaaattatt catataaccg
    37681 ggtatcaaag tgacgcccag ccccatgccc accagattca tggtcaccag aatattcgtt
    37741 gccacctgga cgatatttgg ctggctgttt tcttgcgcaa accacgcttt aacgatcggc
    37801 gcaagcgaac cggaatacgc cggatcggta ctgacgaaat tcacgccatc cagttgcgcg
    37861 gcggtgatct ctttttcatg tgctaaaggg tgatcaaccg gtaacacaac cactaatggt
    37921 tcgtcaaaaa gctccagata atcaatctcc gggctataaa caggatggcg cattaagccg
    37981 acgtcgagtt caccacggcg aattttttcc tcctgttgcg tggtgattaa actcaccagc
    38041 tcaatcaagg tgtctggctg tctgagacga aacatcggta atacttttgg cagtaaattc
    38101 acttccgccg atggcacaaa gccaatggtt aattgtctgt cttcctgaac agttttccgc
    38161 gcccgtaatt tggcattttc cgcttgttcg agaattgcca gcgcgtcctg gagaaaacat
    38221 tctcccgccg ccgtcaacgc gactttgcgc ttatccctca ccagtaacgg aacaccgaca
    38281 cagttttcaa gatcgcggat ctggctgctt aacgaaggct gtgaggtatg cagtttttcc
    38341 gccgcacggg taacgttcag tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt
    38401 tccatcacct tccccttgtt atcgaaaaaa cgtctcagct ggtagaaatt aactatttca
    38461 catattagca accaaccagc aatatcctta tggcacaaaa atagaaggtc aatacatctt
    38521 atctttcagg attaaaaaat atgaccacac cctcagattt gaacatttac caactgattg
    38581 acacccaaaa tggtcgggtc actccgcgta tttataccga cccggatatt taccaactgg
    38641 agcttgagcg tattttcggt cgttgctggc tatttctcgc ccacgaaagc cagatcccaa
    38701 aacccggtga tttctttaac acctacatgg gagaagatgc ggttgtcgta gtgcgtcaga
    38761 aagacggcag catcaaggcg tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt
    38821 atgcagattg cggcaacact cgcgccttta cctgcccgta tcacggctgg tcttatggca
    38881 ttaacggcga gttgatcgat gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta
    38941 aatcccactg ggggctaaac gaagttcctt gtgtggagag ttataaaggg ctgatttttg
    39001 gcaactggga taccagcgca ccgggcctgc gtgattacct tggtgacatt gcctggtatc
    39061 tggatggcat gctggatcgt cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt
    39121 gggtgatcaa ctgtaactgg aaatccccgg cagagcagtt cgccagtgac caatatcatg
    39181 ctctgttcag ccatgcttct gccgttcagg tattaggggc gaaagatgat ggcagcgata
    39241 agcgtctcgg tgatggacaa accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc
    39301 aatttggtca ggacggtcac ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg
    39361 tctgggtcga tggcgcggtt tccagctatt accgcgaaac ctatgccgaa gcagaacaac
    39421 gtttaggtga agtttgcgcc ctgcgcctgg cgggtcataa caatatattc cccacgcttt
    39481 catggctaaa cggcactgcc acactccgcg tctggcatcc gcgcggccct gatcaagttg
    39541 aagtgtgggc gttctgtatt actgacaaag ccgcctccga tgaagttaaa gccgcttttg
    39601 aaaacagcgc cactcgtgct tttggtcctg ctggttttct cgagcaggat gactcggaga
    39661 actggtgtga aatccagaaa ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc
    39721 tggaaatggg gcttggtcag gaaaagcgcc gcgacgacgg cattcctggc attactaact
    39781 atatcttttc agaaactgcc gctcgtggaa tgtaccaacg ctgggccgat cttctgagta
    39841 gcgaaagctg gcaggaagtg ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat
    39901 gagtgcgcaa gtttcactag agttacatct ccgcattagc cagtttctct ttcacgaagc
    39961 cagcttactg gacgactgga aatttcgtga ctggctggcg cagctcgacg aagagattcg
    40021 ttacaccatg cgcaccacag ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc
    40081 accgacaacc tggattttta atgacaccaa agaccagctg gagcggcgaa tcgcccgtct
    40141 ggaaacgggc atggcctggg cagaagagcc gccgtcacgc acccgtcact taatcagcaa
    40201 ctgccagata agcgaaaccg acatcccaaa cgtatttgct gtgcgggtaa attatctgct
    40261 ttatcgggca caaaaagagc gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt
    40321 tcgccgtctg gaagatgaca actggcgctt gctggaacgg gatatcgtcc tggatcaagc
    40381 ggtaatcact tcccataacc tgagtgtact gttctgatga atcgaattta tgcgtgtccc
    40441 gttgcggatg tgccggaggg tgaggctctc cggatcgata cctcgcccgt catcgccctg
    40501 ttcaacgttg gcggcgagtt ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg
    40561 atgtcagaag ggtatctgga agatgacgcc acggtggagt gcccgctaca cgccgccagt
    40621 ttttgcctga aaacggggaa agcgttatgc ctgcccgcca ccgatccgct caccacttat
    40681 ccagtacacg ttgaaggtgg tgacattttc atcgacttac cggaggcgca gccatgagcg
    40741 atctgcataa cgagtccatt tttattaccg gcggcggatc gggattaggg ctggcgctgg
    40801 tcgagcgatt tatcgaagaa ggcgcgcagg ttgccacgct ggaactgtcg gcggcaaaag
    40861 tcgccagtct gcgtcagcga tttggcgaac atattctggc ggtggaaggt aacgtgacct
    40921 gttatgccga ttatcaacgc gcggtcgatc agatcctgac tcgttccggc aagctggatt
    40981 gttttatcgg caatgcaggc atctgggatc acaatgcctc actggttaat actcccgcag
    41041 agacgctcga aaccggcttc cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg
    41101 gcgcaaaagc ctgcgctccg gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt
    41161 caaatgccgc ctggtatcct ggcggcggtg gcccgctgta caccgccagt aaacatgccg
    41221 caaccggact tattcgccaa ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg
    41281 tcggcccgtg tggtatggcc agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa
    41341 cctcgataat gcagtctctg acgccggaga aaattgccgc cattttaccg ctgcaatttt
    41401 tcccgcaacc ggcggatttt acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc
    41461 gcgcattaag cggtgtgatg atcaacgctg atgcgggttt agcgattcgc ggcattcgcc
    41521 acgtagcggc tgggctggat ctttaaggaa gcacgatgaa agaaaaaacg atcattattg
    41581 tcggtggcgt gcaagcggcg gcaatggctg cggcctcgct acgccagcaa gggttcaccg
    41641 gtgagctgca tctgttttcc gatgagcgac atcttcctta tgaacgacct ccactctcga
    41701 aatccatgtt gctggaagat tccccgcagt tacagcaggt gttacccgct aactggtggc
    41761 aggaaaacaa tgttcatctg cattccggtg taaccatcaa aacgctgggt cgcgacacac
    41821 gagagttagt gttaaccaac ggcgaaagct ggcactggga tcagcttttt atagcaaccg
    41881 gcgcggcagc tcgaccgctg ccgttgcttg atgcactggg agaacgctgc tttaccctac
    41941 gccatgccgg tgatgccgcc agactgcgag aagttctgca gcccgaacgg tcagtcgtga
    42001 ttgtcggtgc cggaactatt ggtctggaac tggctgccag cgccacgcag cgcagatgta
    42061 aggtgacagt gattgaactg gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc
    42121 aacgctatct tttacagcgc caccagcagg ctggtgtgcg cattctgctc aataatgcca
    42181 ttgaacatgt ggtcgatggt gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc
    42241 aggctgatgt ggtgatttac ggtattggta tcagcgccaa cgagcaactg gctcgcgagg
    42301 ccaaccttga tactgccaat ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg
    42361 cgatctttgc cggtggcgat gtggcaatca ctcgtcttga taatggtgca ctacaccgct
    42421 gcgaaagctg ggaaaacgcc aataaccagg cgcaaattgc cgctgccgca atgttagggc
    42481 taccgctact gccgccgccg tggttctgga gcgatcagta cagtgataac ttacagttta
    42541 ttggcgatat gcgtggcgat
//
LOCUS       DAJTMK010000012        41823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTMK010000012 DAJTMK010000000
VERSION     DAJTMK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41823
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.012"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..527
                     /locus_tag="OO135_000553"
     CDS             <1..527
                     /locus_tag="OO135_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5396511.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="OO135_000554"
     CDS             893..1960
                     /locus_tag="OO135_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5396512.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASEAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="OO135_000555"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="OO135_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5396513.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGIGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="OO135_000556"
     CDS             4692..5816
                     /locus_tag="OO135_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5396514.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6164
                     /locus_tag="OO135_000557"
     CDS             5889..6164
                     /locus_tag="OO135_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5396515.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6161..6520
                     /locus_tag="OO135_000558"
     CDS             6161..6520
                     /locus_tag="OO135_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5396516.1"
                     /translation="MIKLKTPNTMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OO135_000559"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OO135_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5396517.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OO135_000560"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OO135_000560"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5396518.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGYISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OO135_000561"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OO135_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5396519.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADELTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OO135_000562"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OO135_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5396520.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OO135_000563"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OO135_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5396521.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OO135_000564"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OO135_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5396522.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OO135_000565"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OO135_000565"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5396523.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13764)
                     /locus_tag="OO135_000566"
     CDS             complement(12715..13764)
                     /locus_tag="OO135_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5396524.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13863..15107
                     /locus_tag="OO135_000567"
     CDS             13863..15107
                     /locus_tag="OO135_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5396525.1"
                     /translation="MKHCCKNVVILIPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGT
                     RLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPAL
                     GVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIA
                     LLLTWRLKKRPPEHVPEAASSS"
     gene            complement(15111..15668)
                     /gene="dcrB"
                     /locus_tag="OO135_000568"
     CDS             complement(15111..15668)
                     /gene="dcrB"
                     /locus_tag="OO135_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5396526.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSHDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15741..16406)
                     /locus_tag="OO135_000569"
     CDS             complement(15741..16406)
                     /locus_tag="OO135_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5396527.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILNVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16627..16872
                     /gene="tusA"
                     /locus_tag="OO135_000570"
     CDS             16627..16872
                     /gene="tusA"
                     /locus_tag="OO135_000570"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5396528.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16974..19172)
                     /gene="zntA"
                     /locus_tag="OO135_000571"
     CDS             complement(16974..19172)
                     /gene="zntA"
                     /locus_tag="OO135_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5396529.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCATCARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDGQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19246..19872)
                     /locus_tag="OO135_000572"
     CDS             complement(19246..19872)
                     /locus_tag="OO135_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5396530.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20013..20372
                     /locus_tag="OO135_000573"
     CDS             20013..20372
                     /locus_tag="OO135_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5396531.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRCVSFVGEQ"
     gene            complement(20375..20644)
                     /locus_tag="OO135_000574"
     CDS             complement(20375..20644)
                     /locus_tag="OO135_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5396532.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20634..21230)
                     /gene="rsmD"
                     /locus_tag="OO135_000575"
     CDS             complement(20634..21230)
                     /gene="rsmD"
                     /locus_tag="OO135_000575"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5396533.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21380..22882
                     /gene="ftsY"
                     /locus_tag="OO135_000576"
     CDS             21380..22882
                     /gene="ftsY"
                     /locus_tag="OO135_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5396534.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFI
                     SLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKE
                     EMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDT
                     FRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQN
                     KSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTK
                     LDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22885..23553
                     /gene="ftsE"
                     /locus_tag="OO135_000577"
     CDS             22885..23553
                     /gene="ftsE"
                     /locus_tag="OO135_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5396535.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23546..24604
                     /gene="ftsX"
                     /locus_tag="OO135_000578"
     CDS             23546..24604
                     /gene="ftsX"
                     /locus_tag="OO135_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5396536.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRVKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24849..25703
                     /gene="rpoH"
                     /locus_tag="OO135_000579"
     CDS             24849..25703
                     /gene="rpoH"
                     /locus_tag="OO135_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5396537.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25974..27077
                     /gene="livJ"
                     /locus_tag="OO135_000580"
     CDS             25974..27077
                     /gene="livJ"
                     /locus_tag="OO135_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5396538.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27276..27659)
                     /gene="panM"
                     /locus_tag="OO135_000581"
     CDS             complement(27276..27659)
                     /gene="panM"
                     /locus_tag="OO135_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5396539.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVILSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKH"
     gene            28083..29192
                     /gene="livK"
                     /locus_tag="OO135_000582"
     CDS             28083..29192
                     /gene="livK"
                     /locus_tag="OO135_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5396540.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29240..30166
                     /gene="livH"
                     /locus_tag="OO135_000583"
     CDS             29240..30166
                     /gene="livH"
                     /locus_tag="OO135_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5396541.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30163..31440
                     /gene="livM"
                     /locus_tag="OO135_000584"
     CDS             30163..31440
                     /gene="livM"
                     /locus_tag="OO135_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5396542.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            31437..32204
                     /gene="livG"
                     /locus_tag="OO135_000585"
     CDS             31437..32204
                     /gene="livG"
                     /locus_tag="OO135_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5396543.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32206..32919
                     /gene="livF"
                     /locus_tag="OO135_000586"
     CDS             32206..32919
                     /gene="livF"
                     /locus_tag="OO135_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5396544.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            33316..34633
                     /gene="ugpB"
                     /locus_tag="OO135_000587"
                     /pseudo
     CDS             33316..34633
                     /gene="ugpB"
                     /locus_tag="OO135_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            34731..35618
                     /gene="ugpA"
                     /locus_tag="OO135_000588"
     CDS             34731..35618
                     /gene="ugpA"
                     /locus_tag="OO135_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5396545.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            35615..36460
                     /gene="ugpE"
                     /locus_tag="OO135_000589"
     CDS             35615..36460
                     /gene="ugpE"
                     /locus_tag="OO135_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5396546.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAAWIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            36462..37520
                     /gene="ugpC"
                     /locus_tag="OO135_000590"
     CDS             36462..37520
                     /gene="ugpC"
                     /locus_tag="OO135_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR5396547.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEVELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSN
                     LDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEV
                     YEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKMTLGI
                     RPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGSTLWL
                     HLAENQLHLFDGETGQRV"
     gene            37517..38260
                     /gene="ugpQ"
                     /locus_tag="OO135_000591"
     CDS             37517..38260
                     /gene="ugpQ"
                     /locus_tag="OO135_000591"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5396548.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(38247..38687)
                     /locus_tag="OO135_000592"
     CDS             complement(38247..38687)
                     /locus_tag="OO135_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5396549.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSKPQPDIPLKTIGP"
     gene            38807..40540
                     /gene="ggt"
                     /locus_tag="OO135_000593"
     CDS             38807..40540
                     /gene="ggt"
                     /locus_tag="OO135_000593"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5396550.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIHSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(40578..40862)
                     /locus_tag="OO135_000594"
     CDS             complement(40578..40862)
                     /locus_tag="OO135_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HCR5396551.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(41062..41217)
                     /locus_tag="OO135_000595"
     CDS             complement(41062..41217)
                     /locus_tag="OO135_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396552.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
     gene            complement(41312..41806)
                     /locus_tag="OO135_000596"
     CDS             complement(41312..41806)
                     /locus_tag="OO135_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396553.1"
                     /translation="MMTKTQINKLIKMMNDLDYPFEAPLKESFIESIIQIEFNSNSTN
                     CLEKLCNEVSILFKNQPDYLTFLRAMDGFEVNGLRLFSLSIPEPSVKNLFAVNEFYRN
                     NDDFINPDLQERLVIGDDSISIFTYDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIM
                     DSCS"
BASE COUNT         9806 a        10668 c        11616 g         9733 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgcgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctgaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgatagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctagatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ccgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accttggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tagggctgct gatctccacc tttatgaaaa gccagattgc cgcaattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggaatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattta ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaacactatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata tacccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcagttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggcgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccaccatc
     9541 atcagcgtaa agcactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagc gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataatcc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctggccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 cagtcgatgg cccatcatcc gtggttctaa aatattgccg atgaccatat ggaccactaa
    12961 aaacaatgcg ccgaccagaa tacattcgta aacaccatta aacagcagca cctgaatcat
    13021 tggcggtacg gcggaaatta ccgcgccgat attgggcacg tagttgagca aaaacgccag
    13081 tactgcccac atcagcgcaa actgtacgcc cattagcgcc agccccagcc agacgatgac
    13141 acctgtccat aaactgagta gcgtctttaa cgccagataa tgcgaaacgc ctttaagtgc
    13201 gcggtgtaat cctgcgatgt gaatctgtgg gttattcagc gcaaaacgca ttttgtaagg
    13261 gacgtggcgc acttcaaaca gcataaaaac tacggtcatc accagcaaaa gcacgctcgc
    13321 cattgccccg gaaagcccgg tcattagcgc tgtggtgaag gtaaccactt tttccgagtc
    13381 catccgctgc agcattcgct ccggcgacat atgcaaatta agaaaaggca acatctcctg
    13441 caatttaaaa agtttgcgcg tcagctcctt attaaacttc ggcagcatag agataaattc
    13501 gttaaacgat gccgccagta cgccgaccag cgcggttagt gcgatcagca tcaccaccac
    13561 tacaatcgta atggcaacgg ggcgttgtac tccccgacga ataaaccagg taacgagcgg
    13621 gttgaggacg atggcaaaaa acagcgccag taacagctgc acaatgatat ctgccgctgc
    13681 gtgaatgccc gcgaggatca ctaccagcga ggccaacttg agcagaatgt gcatgcccgt
    13741 tttatcgggt tgaggggttt ccattggggc ttccttgtga ctttttgtgt taagtgtagc
    13801 gggagtcacg ccagcaatat tctgattctt actgctgatt ttcacgtcag cacgtggtaa
    13861 aaatgaaaca ctgttgtaaa aatgtggtga tcctcatacc cgaacccgta gccgaacccg
    13921 cgctaaacgg attgcgcctg aatttgcgca ttgtctccat tgtcatgttt aacttcgcca
    13981 gctacctcac catcgggttg ccgctcgctg tattaccggg ctatgtccat gatgtgatgg
    14041 gctttagcgc tttctgggca ggattggtta tcagcctgca atatttcgcc accttgctga
    14101 gccgtcctca tgccggacgt tacgccgatt tgctgggccc caaaaagatt gtcgtcttcg
    14161 gtttatgcgg ctgctttttg agcggtctgg gatatctgac ggcaggatta accgccagtc
    14221 tgcccgtcat cagcctgtta ttactttgcc tgggacgcgt gatccttggg attgggcaaa
    14281 gttttgccgg aacgggatcg accctgtggg gcgttggcgt ggttggctcg ctgcatatcg
    14341 ggcgagtgat ttcgtggaac ggcattgtca cttacggggc gatggcgatg ggtgcgccgt
    14401 taggcgtcgt gttttatcac tggggcggct tgcaggcgtt agcgttaatc attatgggcg
    14461 tggcgctggt ggccattttg ttggcgatcc cgcgtccgac ggtaaaagcc agtaaaggca
    14521 aaccgctgcc gtttcgcgcg gtgcttgggc gcgtctggct gtacggtatg gcactggctt
    14581 ctgccggatt tggcgtcatc gccaccttta tcacgctgtt ttatgacgct aaaagttggg
    14641 acggtgcggc tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat
    14701 tccctaacgg cattaaccgt atcggcggct taaacgtagc gatgatttgc tttagcgttg
    14761 agataatcgg cctgctactg gttggcgtgg cgactatgcc gtggatggcg aaaatcggcg
    14821 tcttactggc gggggcaggg ttttcgctgg tgttcccggc attaggtgta gtggcggtaa
    14881 aagcggttcc gcagcaaaat cagggggcgg cgctggcaac ttacaccgta tttatggatt
    14941 tatcgcttgg cgtgaccgga ccactggctg ggctggtgat gagctgggcg ggcgtaccgg
    15001 tgatttatct ggcggcggcg ggactggtcg caatcgcgtt attactgacg tggcgattaa
    15061 aaaaacggcc tccggaacac gtccctgagg ccgcctcatc atcttaaaac ttactgaata
    15121 accagcgtat taatgatgtt ttctgcggtg gtctgcgctt tttgctgatc gtcagcgggc
    15181 agcgtaattt gcatggtcag cagttgatta cccacgttac ccagaataac ggaagagtac
    15241 gccgtctggc ctttcgcgga gataatactg tctaactgct gcattttgtg acctttcagc
    15301 tcaatggctt tattggttac cacttgcagc tgcggatcgt ggctacgttg ctgatcttcc
    15361 agacgcttcg ccagcaccgc cagatcttct ttcggatcat cgcccatgat gacgatgact
    15421 gctttctgcc cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt tcccagctta
    15481 ccgctctggt cggtcatatc cgctggcagc gagaaactta acttgccatc aagcaggttg
    15541 acgggattcc cggtagcgtt actttccgcg accgaaccct gcgccgtagc gttagtgtct
    15601 ttatcatcac aggccgcaag ccccataacc agcaggccaa ttccgacata tttaaccaga
    15661 ttgcgcattg acttcttcct ttcgataaac ggccataacg gctcattcat ccatcttatc
    15721 acaactctga taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc
    15781 agtcttttca acagcatatt gagtaatacg ccatacattg gcaggaagaa aacgatactg
    15841 attaacactt tgaaacagta atcgaccagc gcgatttcca tccagtgttc agccataaag
    15901 gcatccgggc tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg
    15961 aacagtgtgg acgctgtcgg tgccagccac cagcggcgac tctgacgcag gcggttaaaa
    16021 acgtgcacgt tgaggatctg tcccagcgcg taggccatga aactggcggt ggcgatacgg
    16081 gcgacaaaca ggttgaagtg ggcgagtgcg ccgaatccct gccaggaacc catatagaat
    16141 agcgacgaga tgacgtagga gatcagcaac gccgggatca tcaccgcaaa gatgatacga
    16201 cgggccagcg gtgcgccaaa aatacgcacg gtcaggtcgg tagcaaggaa aataaacggg
    16261 aagctaaacg cgccccaggt ggtatggaaa cccaaaatgg agacgggaag ctgaaccaga
    16321 tagttactgg aggtgatcac cagcagatga aataacgata accagaacaa cgccttatag
    16381 cgttgagttt gcgagaaaac gttcatattg tacctttttg attaaccatt ggggtgaggg
    16441 aacccaatac gtacgacacg tctgttacct taacataata acgtgcttac ccttttttcg
    16501 agccgcagca tgatactgct ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt
    16561 aacctggttt gcttacggac tcacagggcg taaactagcg ccgttttttt atgtgatgag
    16621 aagaaaatga ccgatctctt ttccagccct gaccacacac tcgacgcact tggcctgcgc
    16681 tgcccggaac ccgtaatgat ggtgcgcaaa actgtgcgca atatgcagcc cggcgaaacg
    16741 ttgctgatta tcgccgacga tccggccact acccgcgata ttcctgggtt ttgtaccttt
    16801 atggaacacg aactggttgc gaaagagacg gatggactgc cttatcgtta tttgattcgt
    16861 aaaggcggtt gatgggggct gattggcttc gatgccgcct tttcccctca ccctaaccct
    16921 ctccccagag gggcgagggg accgatcgcg ctcaatgttg cgatcggttt gccttatctc
    16981 ctgcgcaaca atcttaacgc attcgctgtc accagcaccg tcgcacccgt atctgccagc
    17041 accgccagcc atagtccggt catccctaaa agcgtggtga cgaggaagat ccctttcagt
    17101 cccagcgcaa tggtgatgtt ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc
    17161 atttgcacca ggccgcgcag gtggttatgg gttaatgctg catcagcggt ttccagcgcc
    17221 acgtctgtgc cactgcccat tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg
    17281 ttaataccgt caccgaccat cgccagcggc gcatgttgat tcagctcggt caccgctttg
    17341 actttatctt ccggcaacag gcccgcttta aactccagcc ccagctcccc ggcaattgcc
    17401 gccgctgcgc gtggattatc gccggtgagg atcacccctt tgacgcccag cgcgttcagt
    17461 tcactgatgg cagttgcagc atcggcgcgc agggtatcct gcaatgcaat gatacccagc
    17521 acgtcatcat tacgtactac cagcacaacc gtttgcccgg cgctttccag ttcattaatc
    17581 aaaccagcaa atgcatcagc gggatgtttc ccggcagcgc atatcaacac gcgctcaccg
    17641 ttaacctgtg cttcaatgcc agacccgacc agcgcccgct gtgattcggc ggcgggaatg
    17701 gcgagtgcag caacctgtgc ttcgcgcacg atggcctgcg ccagtggatg cgtcgcgctc
    17761 tgctcgaccg ccgccgccag tgtcagcagt tcagattcac taatacccgt tgccggatga
    17821 atcgcggtaa cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa cgccacctga
    17881 gtaacacgac ctagttgttc cagcgccgct ccgcctttaa tcaacgcccc acgacgcgct
    17941 gctgccgcca gcccggaggt aatcgccgcg ggcgtggaga taactaacgc acacgggcag
    18001 ccaatcagca gcagcgtcag gcctttataa atccactcct gccagctggc ggcaaacagc
    18061 agcggtggca ccagcgtcac cagcagggcg acggccataa tcgctggcgt ataaatacgg
    18121 ctgaaacggt cgataaaccg ctcaatgggg gcgcggcgct cttcggcttc ttcgatcagt
    18181 ttcagaatcc ggtcaatggc gctggcaccc ggttctgaca gcacttccag cgttaccaga
    18241 cggtctacgc tggtggctcc tgcaggaact ttatcgcccg tcgcgcgctc caccggaatg
    18301 gattctccgg tcagggcgct ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg
    18361 gcaggcaaac gcccacctgc ggcgacttca atcacatcgc caggccgcag gctgttaatc
    18421 gccacctctt cccgctcacc gttacgcagg cgcgtggcgg tttctggttt cagcgccatt
    18481 aacgcgctaa cgccctgacg cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc
    18541 aaaaacagca gcaacaccat cgcagcttca gccgttgcgc caataaacag tgcaccaata
    18601 gcggctacgc tcattaaggt ttcaatggcg aagtagctgc cggatttgat cagccgtaat
    18661 gcctgatgag caatcgggta cagcccaacc agcgtggtcg cgataaacgc cagttgcccg
    18721 aacggatgat tgaactgctc cagaccccag ctgattgcca tcatcacgat tagtgtaatc
    18781 agcggcagat tctctttcag gcgcgatgct tgtggctcgt cggcggcctg cccatcgcgc
    18841 agggaatagc ccgctttttg caccgcagat tcaacttgtg cacgaatgtc attgtcggca
    18901 tcaaccacca gtttttcggt ggcgaacaac acctgcacct gattcacacc tgcaagctgg
    18961 cgcacggcat tttctacctt gcgcgcacag gtggcgcagt ccatgccact gactttccag
    19021 ctatagcggg tgccggagac gttttcagag agcgttggcg agctggagca tgcgccgtcg
    19081 cagcaacagt cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg
    19141 gctttcttgc cgtgattgtc aggtgtcgac atggcatact ccggttaagt tttttctcat
    19201 taaccgaagg atacactctg gagtcgactc cagagtcaag ttttatcaga gatacagtga
    19261 gcggacgatg aggaagtgac cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg
    19321 gaagcgtcgg cgatagtggc tccccagcca gacaaagtta ctgataaaca gcaacgaagc
    19381 gccaacaaac gcagagagcg ccggagcggt cggacggaag aaccacagtt cacctgccag
    19441 ccacaccatc accagcgtca tgccgataaa cgtgcagata ggccaacgat actcttccag
    19501 ccgcgtccag ataatcgcca gtaacagcgc acccagaacc agcaacacca gtggcagagg
    19561 ccagaagaaa gagagcgtca tctgactggc gaaatagatg gtgtacagca ggtgcgagag
    19621 gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc aacagggtta gcgcatcgcc
    19681 cagcagtgag gcgcacagcc ctgccagcac cagatagcta atggcgtcga acatcggcgc
    19741 ttgccaggcc agtaacagca ggagaagaag ggttaacggt ttaaacaccc agcgttgcca
    19801 ggttggccca cgatacgatg catccacaga tagccatgcg gaaagacaga cagcgataaa
    19861 cgaccaaagc atcttagttc cttgaattcg ttattttttc gtaagcggaa tgcctacggt
    19921 cttgcttcca gtttagtggc gagggtggac ggatgacaac accataactg gcagggtatg
    19981 cttatttccg cattttccga tgagggataa agatgagcaa accacctctt ttctttattg
    20041 ttatcattgg cttaattgtc gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa
    20101 aagctgataa tgatatggct ccgctccagc aaaagctggt ggtggtgagc aacaagcggg
    20161 aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt gactccggca ggcaccagta
    20221 tgcgctatga ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg
    20281 acgcccagca gtaccatgcc ctgacagtgg gcgataaagg tacgctgagc tataaaggaa
    20341 cgcgctgtgt tagctttgtt ggcgaacaat aaggttattt tttaacttta aatttcttct
    20401 gccagaccag cagttcaaac acgccgaaaa ggaaaatccg caccttttcg gcggtggtca
    20461 tctgcgggcc atctttcggt aaagtggatt tcaatagcgc cagctgcata ccatgcatca
    20521 gcacggtaaa aatcagcgcc acgttaacga aaatattcag cgggcgcggg aagggatgca
    20581 ccagattgag gattaaaaat ccccaaacgc agagcattaa caaacgacca atattaatca
    20641 gcatcacttt ctccttgtgc ttcgcgttga tacagccgat aagccacctg acccgccact
    20701 ttttcccgat gtaatgacca gtttgccgga acagtgggca gaccgttttc gacttcgctt
    20761 tcgacataaa tcagggcttc gtcagccagc cagccgttat cttccagtaa atttatcgtc
    20821 tcttctaaca agccacggcg gaacggtgga tcgacaaaca cgatattatg cggtgtacct
    20881 ttttgcgcca ggaatgacat cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt
    20941 gtcgccagat tcttaattaa ctgctgagaa accgcgcgat ccatctcaat caacgtggcc
    21001 cccgcagcgt agcgcgataa cgcttccagt cccagcgcgc cgctcccggc gaagcaatcc
    21061 agacattggg cgtcaacaat gaccggagcc agccagttaa acaacgtttc gcgtacgcgg
    21121 tcggtggtgg ggcgcagacc tgggctatca ggaaccggga gtttacggcc tcgccactgc
    21181 ccgccaataa tgcggatttg gccgctgccg gaatgattcg gttttttcat gataaattgc
    21241 tcaatccgcc agataacaca tacttgcagg cggtgatgtg aattaagtta agtgatagac
    21301 tatttcatca tttttttagc tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca
    21361 gcgaggagtg tagtcgcaaa tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg
    21421 ctttggtcaa aaagagcaga ccccggaaaa agagacagaa gttcagaatg aacaaccggt
    21481 tgtagaagaa atcgttcagg cgcaagagcc tgcgaaggct tctgaacaag ccgtcgaaga
    21541 gcaaccgcag gcgcatactg aagccgaggc ggaaactttt gctgccgacg ttgtggaagt
    21601 cactgaacag gttgctgaaa gtgaaaaagc gcagcctgaa gcggaagtcg ttgcacagcc
    21661 ggaaccggtc gtagaagaaa cgccggagcc agtggctatc gaacgtgaag agctgccgtt
    21721 gccggaagac gtcaacgccg aagaggtttc gccagaagag tggcaggctg aagcggaaac
    21781 cgtagagatt gtcgaagcgg cggaagaaga agcggctaaa gaagaaatca ccgacgaaga
    21841 gctggaagca caggcgctgg ctgccgaagc ggcagaagag gcagaagagg cggtgatggt
    21901 ggttcctccg gtagaagagc agccggtgga agaaatcgct caggagcagg aaaaaccgac
    21961 caaagaaggt ttcttcgcgc gcctgaaacg cagcctgtta aaaaccaaag aaaatctcgg
    22021 ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt ttgaggagct
    22081 ggaagaacag ctgttgatcg ccgatgtggg cgtggaaacc acacgtaaaa ttatcaccaa
    22141 tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct atggcctgct
    22201 gaaagaagag atgggcgaga ttctggcgaa agtcgatgag ccgctgaatg ttgaaggtaa
    22261 aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacta cgacgattgg
    22321 taagctggca cgtcagtttg agctgcaggg taaatcggtg atgctggcgg cgggcgatac
    22381 tttccgtgca gcagcggttg aacagcttca ggtctggggt cagcgcaaca atattccggt
    22441 gattgcccag cataccggtg cggattccgc ctctgttatc ttcgacgcca ttcaggcagc
    22501 taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc agaacaaatc
    22561 gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg acgttgaagc
    22621 gccgcatgaa gttatgctga ctattgatgc cagtaccggg cagaatgcgg taagccaggc
    22681 caaactgttc cacgaagccg ttggcttaac cggcatcacg ctaacgaaac tggacggcac
    22741 ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta tccgctacat
    22801 tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact ttatagaggc
    22861 actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa ggcttatctc
    22921 ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga gatggcgttt
    22981 ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg tgggattgag
    23041 cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct gaaaaaccgt
    23101 gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca tctactgatg
    23161 gaccgtactg tctacgataa cgtggcgatc ccgctgatta tcgctggtgc cagcggtgac
    23221 gatattcgtc gccgggtgtc ggcggcactg gataaagtcg ggctattgga caaagcgaag
    23281 aacttcccta ttcagctttc cggcggtgaa caacagcgtg ttggcattgc ccgcgcggtg
    23341 gtgaataagc ccgcggtact gctggcggat gaaccgaccg gtaacctaga cgacgcgttg
    23401 tcggaaggga ttctacgtct gtttgaagag tttaaccgcg ttggggtaac cgtattgatg
    23461 gcaacgcacg acatcaacct gatttcacgg cgttcctatc gcatgctcac cctgagcgat
    23521 ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc atattcggca
    23581 gtttggtggg cgtctggatc gcttccgtaa atcggtcggc ggctcaggcg acggcggtcg
    23641 taacgcaccg aaacgcgtga aatcctcgcc aaaaccggta aatcgcaaaa ccaacgtttt
    23701 caacgaacag gtgcgctatg ccttccacgg cgcattgcag gatctgaaaa gcaaaccgtt
    23761 cgccacgttt ttaacggtga tggttatcgc catctccctg acgctgccca gcgtctgtta
    23821 tatggtgtac aaaaacgtta atcaggcggc gacgcagtat tatccgtcac cgcaaatcac
    23881 tgtttacttg caaaaaacgc tggacgatga cgccgctgcg ggcgtggtgg cacagttgca
    23941 ggccgagcaa ggcgtggaga aagtgaacta tctttctcgt gaagacgcac tgggcgagtt
    24001 ccgtaactgg tctggttttg gtggtgcgct ggatatgctg gaagaaaacc cgcttccggc
    24061 agtggcggtg gtgatcccga aactcgattt tcaggggacg gaatcactca atacgctgcg
    24121 tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca gctggtttgc
    24181 ccgtctggcg gcgttgaccg ggctggtcgg gcgcgtttcg gcgatgatcg gcgtgttgat
    24241 ggtggcggca gtgttcctcg tcatcggcaa cagcgtacgt ctgagcatct ttgctcgccg
    24301 tgactccatc aacgtgcaga aactgattgg tgcgacggat ggtttcatcc ttcgcccgtt
    24361 cctgtatggt ggcgcactgc tgggattttc tggcgcattg ttgtcattaa ttttgtcaga
    24421 aattctggtg ctgcgattgt catcggcggt tgcggaagtg gcacaggttt tcggaacgaa
    24481 ttttgatatc aatggtttat cgttcgatga atgcctgcta ttgctgctgg tatgctcgat
    24541 gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact ttacgcctga
    24601 ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg aaagagatcc
    24661 cgttgtctct tcccggtatt tcatctctat gtcacatttt gtgcgtaatt tattcacaag
    24721 cttgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga taacctcgtt
    24781 gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata tcgattgaga
    24841 ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc aacctggatt
    24901 cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag cgggcgctgg
    24961 ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc ctgtctcacc
    25021 tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca caggcggatt
    25081 tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgtttcaac ccggaagtgg
    25141 gtgtgcgcct ggtctccttc gccgttcact ggatcaaagc agagatccac gaatacgttc
    25201 tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcagcgcaaa ctgttcttca
    25261 acctgcgtaa aactaagcag cgtctgggct ggtttaacca ggatgaagtc gaaatggtgg
    25321 cccgtgaact gggcgtaacc agcaaagacg ttcgtgagat ggaatcacgt atggcggcac
    25381 aggacatgac ctttgacctg tcttccgacg acgattccga cagccagccg atggcaccgg
    25441 tgctctatct gcaggataaa tcatctaact ttgccgacgg catcgaagat gataactggg
    25501 aagagcaggc ggcaaaccgt ctgaccgacg cgatgcaagg tctggacgaa cgcagccagg
    25561 acatcatccg cgcgcgctgg ctggacgaag acaacaagtc cacgttgcag gaactggctg
    25621 accgttacgg tgtttccgct gaacgtgtac gccagctgga aaagaacgcg atgaaaaaac
    25681 tgcgcgctgc tatagaagcg taatttccgc tattaagcag agaaccctgg atgagagtcc
    25741 ggggtttttg ttttttgggc ctctacaata atcaattccc cctccggcaa aacgtcaatc
    25801 cccacgcaga ttgttaataa actgtcaaaa tagctattcc aatatcataa aaatggggta
    25861 tgttttagca gagtatgctg ctaaagcacg ggtagtcatg cataaaacga aataaagtgc
    25921 tgaatcacaa caccacaaca cacgtaataa ccagaagaat ggggattctc aggatgaata
    25981 taaagggtaa agcgttactg gcaggatgta tcgcgctggc attcagcaat atggctctgg
    26041 cagaagatat taaagtcgcg gtcgtgggcg caatgtccgg tccggttgcg cagtacggtg
    26101 accaggagtt taccggcgca gagcaggcgg ttgcggatat caacgctaaa ggcggcatta
    26161 aaggcaacaa actgcaaatc gtaaaatatg acgatgcctg tgacccgaaa caggcggttg
    26221 cggtggcgaa caaagtcgtt aacgacggca ttaaatatgt gattggtcac ctctgttctt
    26281 catcaacgca gcctgcgtct gatatctacg aagacgaagg cattttaatg atcaccccag
    26341 cggcaaccgc gccggagctg accgcccgtg gctatcagct gatcctgcgc accaccggcc
    26401 tggactccga ccaggggcca acggcggcga aatatattct tgagaaagtg aaaccgcagc
    26461 gtattgctat cgttcacgac aaacagcaat acggcgaagg tctggcgcga gcggtgcagg
    26521 acggcctgaa gaaaggcaat gctaacgtgg tgttctttga tggtatcacc gccggggaaa
    26581 aagatttctc aacgctggtg gcgcgtctga aaaaagagaa tatcgacttc gtttactacg
    26641 gcggttatca cccggaaatg gggcaaatcc tgcgtcaggc acgcgcggca gggctgaaaa
    26701 ctcagtttat ggggccggaa ggtgtggcta acgtttcgct gtctaacatt gcgggcgaat
    26761 cagcggaagg gctgctggtg accaagccga agaactacga tcaggttccg gcgaacaaac
    26821 ccattgttga cgccatcaaa gcgaaaaaac aggacccaag cggcgcattc gtttggacca
    26881 cctacgccgc gctgcaatct ttgcaggcgg gcctgaatca gtctgacgat ccggctgaga
    26941 tcgccaaata cctgaaagcg aactccgtgg ataccgtgat gggtccgctg acgtgggatg
    27001 agaaaggtga tctgaaaggc tttgagttcg gcgtatttga ctggcacgcc aacggcacgg
    27061 cgaccgatgc gaagtaatca ttaatcggca actttgggtt gccgccaaat tgctaatatc
    27121 gagtacgttg cttcatgccg gatgcggcgt aaacgcctta tccggcctac aagatccaaa
    27181 gaaatcagta aattgcaaca cacattgtag gcctgataag cgtagcgcat caggcaattt
    27241 ctcgttggtc atcacttttg aaatcaactt gacgattaat gcttctccca gccgccctgt
    27301 tgcgccgtaa atcccagtgc ctgcataaac gccgtcatca caccgcgatc ttccacgcct
    27361 gcatccgcca tccaccagca tgaaacgcca ggattgttac gcaaaacctc ttccagcaga
    27421 tattgcccca caccgcgacg tcgggtgact tcccgcacac gcagggaatc cagtgctccc
    27481 tcggtgccgc ttaagattac ccgtacggca gcgagcaggc gctcgttaaa acgcgcggcg
    27541 tagatacggt ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt
    27601 tgcaggtcaa tccggtcctg gtcgctaaag ttttctaatc gaatgatggt cagcttcatg
    27661 ggtaacccgt gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct
    27721 ttctcttttt tatttctttt ggcaggtgat taatttttta acagcaataa ttacaaagtt
    27781 aaaacattag agaatgaaaa atacccagca taatcccctg aatggtagtg aattattccg
    27841 ccctttgtgc cgttatttta tgctgacaaa ggcacttttt tctgtttatc tatcaataaa
    27901 ttcagaatat tatctgttct taatcgactg aaagataggg attttaatca ctattatcac
    27961 aaaatactgc gctaaaccct taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac
    28021 agtctgattt gttaacacat aaaaacaaag caacacaaca tcacgaatgg ggatttttga
    28081 ctatgaaacg gaatgcgaaa actatcatcg cagggatgat tgcactggca atttcacaca
    28141 ccgctatggc tgacgatatt aaagtcgccg ttgtcggcgc gatgtccggc ccgattgccc
    28201 agtggggcga tatggaattt aacggcgcgc gtcaggcgat taaagacatt aatgccaaag
    28261 ggggaattaa gggcgacaag ctggttggcg tggaatatga cgacgcctgc gacccgaaac
    28321 aagccgttgc ggtcgccaac aaaatcgtta atgacggcat taaatacgtt attggtcatc
    28381 tgtgttcttc ttctacccaa cctgcatcag atatctacga agacgaaggt attctgatga
    28441 tctcaccggg agctaccaac ccggagctga cccaacgcgg ttatcaacac attatgcgta
    28501 ctgccgggct ggactcttcc caggggccaa cggcggcaaa atacattctt gagacggtga
    28561 agccccagcg catcgccatc attcacgaca aacaacagta tggcgaaggg ctggcgcgtt
    28621 cggtgcagga cgggctgaaa gcggctaacg ccaacgtcgt cttcttcgac ggtattaccg
    28681 ccggggagaa agatttctcc gcgctgatcg cccgcctgaa aaaagaaaac atcgacttcg
    28741 tttactacgg cggttactac ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg
    28801 gcctgaaaac tcagtttatg gggccggaag gtgtgggtaa cgcatcattg tcgaatattg
    28861 ccggtgatgc tgccgaaggc atgttggtca ctatgccaaa acgctatgac caggatccgg
    28921 caaaccaggg catcgttgat gcgctgaaag cagacaagaa agatccgtcc gggccttatg
    28981 tctggatcac ctacgcggcg gtgcaatctc tggcgactgc ccttgagcgt actggcagcg
    29041 atgagccgct ggcgctggtg aaagatttaa aagctaacgg tgcaaacacc gtgattgggc
    29101 cgctgaactg ggatgaaaaa ggcgatctta agggatttga ttttggtgtc ttccagtggc
    29161 acgccgacgg ttcatccacg gcagccaagt gatcatccca ccgcccgtaa aatgcgggcg
    29221 ggtttagaaa ggttacctta tgtctgagca gtttttgtat ttcttgcagc agatgtttaa
    29281 cggcgtcacg ctgggcagta cctacgcgct gatagccatc ggctacacca tggtttacgg
    29341 cattatcggc atgatcaact tcgcccacgg cgaggtttat atgattggca gctacgtctc
    29401 atttatgatc atcgccgcgc tgatgatgat gggcattgat accggctggc tgctggtggc
    29461 ggcgggattc gtcggcgcaa tcgtcattgc cagcgcctac ggctggagta tcgaacgggt
    29521 agcctatcgc ccggtgcgta actctaagcg cctgattgcg ctcatctcgg caatcggcat
    29581 gtccatcttc ctgcaaaact acgttagcct gactgaaggt tcgcgtgacg tggcgctgcc
    29641 cagtctgttt aacggtcagt gggtggtggg gcatagcgaa aacttctctg cctctattac
    29701 caccatgcag gcagtgatct ggattgttac tttcctcgcc atgctggcgc tgacgatttt
    29761 cattcgttat tcccgtatgg ggcgcgcctg ccgtgcctgc gcggaagatc tgaaaatggc
    29821 gagcctgctt ggcatcaaca ccgaccgggt gattgctttg acctttgtta ttggcgcggc
    29881 gatggcggca gtggctggcg tactgctcgg tcagttctac ggcgtcatta acccctatat
    29941 cggctttatg gccgggatga aagcctttac cgctgcggtg ctcggtggga ttggcagcat
    30001 tccaggggcg atgattggtg ggctgatcct ggggattgcg gaggcgctct cttctgccta
    30061 tctgagtacg gaatataagg atgtggtttc atttgccctg ctgattctgg tgctgctggt
    30121 gatgccgacc ggtattctgg gtcgcccgga ggtagagaaa gtatgaaacc gatgcatatt
    30181 gcaatggcgc tgctctctgc cgcgatgttt tttgttctgg cgggcgtctt tatgggcgtg
    30241 caactggagc tggatggcac caaactggtg gtcgacacgg cttcagacat ccgttggcag
    30301 tgggtgttta tcggcacggc ggtggtcttt ttcttccagc ttttgcgacc ggctttccag
    30361 aaagggctaa aaagcgtttc cggaccgaag tttattctgc ccgcgattga tggctccacg
    30421 gtgaagcaga aactgttcct cgtggcgctg ttggtgcttg cggtggcgtg gccgtttatg
    30481 gtgtcacgtg ggacggtgga tatcgccact ctgaccatga tctacattat cctcggtctc
    30541 gggctgaatg tggttgttgg tctttctggt ctgctggtgc tggggtacgg cggtttttac
    30601 gccatcggcg cttacacctt tgcgctgctc aatcactatt acggcttggg cttctggact
    30661 tgtctgccga ttgccgggtt aatggcagcg gcggcgggct tcctgctcgg ctttccggtg
    30721 ctgcgtctgc gcggtgatta tctggcgatc gttaccctcg gcttcggcga aattgtgcgc
    30781 atattgctgc tcaataacac cgaaattacc ggcggcccga acggaatcag tcagatcccg
    30841 aaaccgactt tcttcggcct tgagttcagc cgtacagccc gtgaaggcgg ttgggacacg
    30901 ttcagtaatt tctttggcct gaaatacgat ccctccgatc gcgtcatctt cctgtatctg
    30961 gtggcgttgc tgctggtggt gctaagcctg tttgtcatta accgcctgct gcggatgccg
    31021 ctggggcgtg cgtgggaagc gttgcgtgaa gatgagatcg cctgccgttc gctgggctta
    31081 agcccgcgtc gtatcaagct gaccgctttt accatcagtg ccgcgtttgc cggttttgcc
    31141 ggaacgctgt ttgctgcgcg ccagggcttt gtcagcccgg aatccttcac ctttgctgaa
    31201 tcggcgtttg tactggcgat agtggtgctc ggcggtatgg gatcgcagtt tgcggtgatt
    31261 ctggcggcaa ttttgctggt ggtgtcgcgc gagttgatgc gtgatttcaa cgaatacagc
    31321 atgttaatgc tcggtggttt gatggtgctg atgatgatct ggcgtccgca gggcttgctg
    31381 cccatgacgc gcccgcaact gaagctgaaa aacggcgcag cgaaaggaga gcaggcatga
    31441 gtcagccatt attatctgtg aacggcctga tgatgcgctt cggcggcctg ctggcggtga
    31501 acaacgtcaa tcttgaactg tacccgcagg agatcgtctc gttaatcggt cctaacggtg
    31561 ccggaaaaac cacagtcttt aactgcctga ccggattcta caaacccact ggaggcacca
    31621 ttttactgcg cgatcagcat ctggaaggtt taccggggca gcaaattgcc cgcatgggcg
    31681 tggtgcgtac cttccagcat gtgcgtctgt tccgtgaaat gacggtgatt gaaaacctgc
    31741 tggtggcgca gcatcagcaa ctgaaaaccg ggctgttctc tggcctgttg aaaacgccat
    31801 ccttccgtcg cgcccagagc gaagcgctcg atcgcgccgc gacctggctt gagcgcattg
    31861 gtttgctgga acacgccaac cgtcaggcga gtaacctggc ctatggtgac cagcgccgtc
    31921 ttgagattgc ccgctgcatg gtgacgcagc cggagatttt aatgctcgac gaacctgcgg
    31981 caggtcttaa cccgaaagag acgaaagagc tggatgagct gattgccgaa ctgcgcaatc
    32041 atcacaacac cactatcttg ttgattgaac acgatatgaa gctggtgatg ggaatttcgg
    32101 accgaattta cgtggtcaat caggggacgc cactggcaaa cggtacgccg gagcaaatcc
    32161 gtaataaccc ggacgtgatc cgtgcctatt taggtgaggc ataagatgga aaaagtcatg
    32221 ttgtcctttg acaaagtcag cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc
    32281 ctgcatatca atcagggcga gattgtcacg ctgattggcg cgaacggggc ggggaaaacc
    32341 accttgctcg gcacgttatg cggcgatccg cgcgccgcca gcgggcgaat tgtgtttgat
    32401 gataaagaca ttaccgactg gcagacggcg aaaatcatgc gcgaagcggt agcgattgtc
    32461 ccggaagggc gtcgcgtctt ctcgcggatg acggtggaag agaacctggc gatgggcggc
    32521 ttttttgccg aacgcgacca gttccaggag cgcataaagt gggtgtatga actgtttcca
    32581 cgtctgcatg agcgccgtgt tcagcgggca ggcaccatgt ccggcggtga gcaacaaatg
    32641 ctggcgattg gtcgcgcgct gatgagcaac ccgcgtttgc tgctacttga tgagccatcg
    32701 ctcggtcttg cgccgattat catccagcaa attttcgaca ccatcgagca gctgcgcgag
    32761 caggggatga ctatcttcct cgtcgagcag aacgccaacc aggcgctaaa gctggcggat
    32821 cgcggctacg tgctggaaaa cggccatgtg gtgctctccg ataccggcga cgcgctgctg
    32881 gcgaacgaag cagtgagaag tgcgtattta ggcgggtaat aacacgttga ttgataagga
    32941 gtcaaaagac tcctttgaga caggtgacaa atgtaaaatt gcctgatgcg ctgcgcttat
    33001 caggcctact gggtgagttg caatatattg aatttgcacg atcttgtagg ccggataagg
    33061 cgtttacgcc gcatccggca tgaaacgatg agtaatctgt agagtttgat tcagaccttc
    33121 tatttttccg cttatccgtg ccccatctcc cattttccct cacccacgcc gtcaccgcct
    33181 tgtcatcttt ctgacacctt actatcttac aaatgtaaca aaaaagttat ttttctgtaa
    33241 ttcgagcatg tcatgttacc ccgcgagcat aaaacgcgtg aattcgcgca ttcggtacaa
    33301 caagagagat aaacgatgaa accgttacat tatacagctt cagcactggc gctcggactg
    33361 gcgttaatgg ggaatgcaca ggcagtgacg accattccgt tctggcattc tatggaaggg
    33421 gaactgggta aagaggtgga ttctctggcc caacgtttta acgccgaaaa tccggattac
    33481 aaaattgtac cgacctataa aggcaactac gaacagaatt taagcgcggg gattgccgca
    33541 tttcgtaccg gcaacgctcc ggctattttg caggtttatg aagttggcac cgccaccatg
    33601 atggcatcga aagccattaa accggtaata tgacgtgttt aaagaggcgg ggattcaatt
    33661 cgatgagtcg cagtttgtgc cgacggtttc cggttactac tccgacagca aaacgggcca
    33721 cttactctcc cagccattca acagctcgac tcccgttctc tattacaaca aagacgcctt
    33781 caagaaagcg gggttagacc cggcacagcc gccgaaaacc tggcaggatc tggcggacta
    33841 tgccgcgaaa ctgaaagcct ccggcatgaa gtgcggctac gccagcggct ggcagggctg
    33901 gatccaactg gaaaacttta gcgcttggaa cggtctgccg tttgccagca aaaacaacgg
    33961 ctttgacggc acagatgcgg tgctggagtt caacaagccg gagcaggtga aacacatcgc
    34021 catgctcgag gagatgaaca agaagggcga cttcagctac gtcggtcgta aggatgaatc
    34081 caccgagaag ttctataacg gtgattgcgc gatgaccacc gcctcttccg gttctcttgc
    34141 caacattcgc gagtacgcca aatttaacta cggcgtaggc atgatgcctt acgacgccga
    34201 tgcgaaagat gcgccacaaa acgccattat cggcggagcc agcctgtggg tgatgcaggg
    34261 taaagataaa gaaacgtata ccggtgtggc gaagttcctc gatttcctcg cgaagccaga
    34321 aaacgctgcc gagtggcatc agaaaaccgg ttatctgcca atcaccaaag cagcgtatga
    34381 cctgacccgt gagcagggct tttatgagaa aaacccaggg gcggataccg cgacgcgtca
    34441 gatgctgaat aagccgccgt tgccgttcac caaagggctg cgtctgggca acatgccgca
    34501 gatccgcgtg attgtggatg aagagctgga gagcgtgtgg accggtaaga agacaccaca
    34561 gcaggcactg gataccgccg ttgagcgtgg gaaccagtta ctgcgccgct ttgagaaatc
    34621 gacgaagtct taatcagtgt aatgtcggat gcgtttcgct tatctgacct ggcatcgcgt
    34681 gtaggccgga taagcgaagc gcatccggca cagttcagga attaaccgta atgtcatcat
    34741 cccgtccggt gttccgctcg cgctggctgc cttatctgct ggtcgcgccg cagctcatca
    34801 tcaccgttat cttttttatc tggcctgcgg gcgaagcgtt gtggtactcg ctgcaaagcg
    34861 tcgatccgtt tggtttctcc agccagtttg tcggtctgga taacttcgtc acgctgttcc
    34921 atgacagcta ctatctagac tccttctgga cgacgataaa attcagcacc tttgtcaccg
    34981 tcagcggttt gctggtatcg ctgttctttg cggcgctggt ggagtacatc gtgcgcggca
    35041 gccgtttcta tcaaaccttg atgctgctgc cgtatgccgt ggctcccgcc gttgccgccg
    35101 tattgtggat cttcctgttt aaccccggtc gcgggctgat cacccatttt ctcgctgagt
    35161 tcggctacga ctggaaccac gcgcaaaaca gcggtcaggc aatgtttctg gtggtgtttg
    35221 cctcggtgtg gaagcaaatc agctacaact ttctgttctt ctatgccgcg ctgcactcca
    35281 ttccccgttc gttgatcgaa gccgcagcca tcgacggtgc agggccgatt cgccgcttct
    35341 ttaagattgc gctgccgctt atcgccccgg tgagtttctt cctgctggta gtgaacctgg
    35401 tgtatgcctt cttcgacacc ttcccggtga tcgacgccgc cacgtccggc gggccggttc
    35461 aggccaccac gacgctgatt tataagatct accgcgaagg ttttaccgga ctggatctgg
    35521 cttcgtctgc cgcacagtcg gtggtgttga tgttcctcgt catcgtgctg acggtggtgc
    35581 agttccgcta tgttgaaagc aaggtgcgtt accaatgatt gagaaccgtc cgtggctgac
    35641 gatattcagc cataccatgc tgatcctcgg gatcgcggtg atcctcttcc cgctgtacgt
    35701 ggcgtttgtc gcggcgacgc tggataaaca ggccgtctat gccgcgccga tgacgctcat
    35761 ccccggcaca catctgctgg aaaatatcca caacatctgg gttaacgggg taggtacgaa
    35821 tagcgcgccg ttctggcgga tgttgcttaa cagctttgtg atggcgttca gcattacgct
    35881 cggcaaaatt accgtctcga tgctctcggc atttgccatt gtctggtttc gttttccgct
    35941 acgtaacctc ttcttctgga tgatttttat caccctgatg ctgccggttg aagtacgtat
    36001 cttcccgacg gtggaagtca tcgccaacct gaagatgctc gacagctacg ccggtttaac
    36061 gctgccgctg atggcctcgg cgaccgccac gtttctgttt cgccagttct ttatgacgct
    36121 gccggatgaa ctggtggaag cggcgtggat cgacggcgca tcgccaatgc gcttcttttg
    36181 cgacatcgtt tttccgctct ccaaaactaa tctggcggcg ctgtttgtga tcacctttat
    36241 ttacggctgg aaccagtatt tgtggccgtt gttgattatt accgacgtgg atctcggcac
    36301 caccgtggca gggatcaaag ggatgatcgc cactggcgaa ggcaccacgg aatggaactc
    36361 agtgatggcg gcgatgttgt taaccctgat cccaccagta gtgattgttt tagtgatgca
    36421 gcgtgccttc gtgcgcggcc tggtcgatag tgagaaataa gatggcagga ctgaaattac
    36481 aggcagtaac caaaagctgg gatggcaaaa cccaggtcat taaaccgctg acccttgatg
    36541 tggctgatgg cgaatttatc gtgatggtcg ggccgtctgg ctgcgggaaa tcgacgctgc
    36601 tgcgtatggt tgccgggctg gagcgggtga ccgaaggcga tatctggatc aacgaccagc
    36661 gcgtgactga aatggagccg aaagatcgcg ggattgcgat ggtgttccag aactacgcgc
    36721 tttatccgca tatgagcgtc gaagaaaaca tggcgtgggg gcttaaaatt cgcggcatgg
    36781 gcaagcagca aattgccgag cgcgttaaag aagtggagct ggacggtctg ctcaaacgtc
    36841 gcccgcgtga gctatccggc ggtcagcgtc agcgtgtagc gatgggccgc gcgattgtgc
    36901 gcgatccggc ggtgttcctg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg
    36961 tgcagatgcg tcttgaactg caacagttgc accgtcgcct gaaaacgact tcactctacg
    37021 ttactcacga tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggcg
    37081 gcgttgccga acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg
    37141 tagcgagttt tatcggcagt ccggcgatga acctgctgac aggccgcgtg aataacgaag
    37201 gcacgcattt cgaactggac ggcggtattg agctgccgct aaacggtggc taccgtcagt
    37261 atgccgggcg taaaatgact ctcggcattc gcccggaaca tattgtgtta agctcgcagg
    37321 cagaaggcgg cgtaccgctg gtgatggaca cgctggagat cctcggcgca gataacctgg
    37381 cgcacggacg ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga
    37441 cggcaggcag cacgctgtgg ctgcatctgg cggaaaatca gctgcatctt tttgatggtg
    37501 aaacaggaca acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg
    37561 cggtaagctg gccccggaaa acaccctggc ggcaatcgac gtcggggcaa aatacggtca
    37621 taagatgatc gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga
    37681 cgacaatctc gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga
    37741 tttactgcgc gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc
    37801 gttgctttcg caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat
    37861 caaacccacc actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca
    37921 actgtgggcc ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc
    37981 tgcacagctg gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga
    38041 ctggcgcgaa ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact
    38101 cgataaagcg cgagtgatgc agttgaaaga cgccggatta cggattctgg tttataccgt
    38161 caataaaccc cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga
    38221 tgcgattgac gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc
    38281 tgcggcttgc tgggctgatt cagcatgtcg ccgtttctcc tctccggcag catatgctgc
    38341 tgattcaacg aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc
    38401 agcatttgct gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa
    38461 ttattgttta tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc
    38521 tgattcagca tccctttttg ctggatttgc tgagtttgca tctgggtttg catccgctgc
    38581 tggctgggta tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca
    38641 aagccgacaa acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct
    38701 ctgaagatat cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat
    38761 ataccaggct tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac
    38821 gtttttacgc cgggtagcca ttgctgctct gctctcagga agttgtttta gcgccgtcgc
    38881 cgcgcctcct gtttcttacg gcgtggagga agatatcttc catccggtac gcgcgaaaca
    38941 ggggatggtg gcgtctgtcg atgccactgc cacccaagtg ggggtagata ttctaaagga
    39001 gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg taacgcatcc
    39061 gcaggcaggg aatctgggcg gtggtggttt tatgctgatc cactcgaaaa atggcaatac
    39121 caccgctatc gatttccgcg agatggcacc cgctaaagcg acccgcgata tgttccttga
    39181 tgaccagggc aacccggaca gcaaaaaatc actcacttcg catctagctt ccggcacgcc
    39241 cggtacggtc gctggtttct cgctggcgtt ggataagtac ggcactatgc cgttgaacaa
    39301 agtggtgcag cccgcgttca aactggcacg cgatggtttt atcgttaacg atgctttggc
    39361 agacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca gcaaagctat
    39421 cttctggaaa gagggcgaac cgctgaaaaa gggcgacaag ctggtgcagg cgaacctggc
    39481 aaagagcctg gagatgattg ccgaaaacgg cccggacgaa ttctataaag gcacgatagc
    39541 ggaacagatt gcccaggaga tgcagaaaaa cggtggcttg atcactaaag aagatttagc
    39601 ggcgtataaa gcggtcgaac gcacaccgat aagtggcgat tatcgcgggt atcaggttta
    39661 ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca atattctgga
    39721 aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa tcatggcaga
    39781 agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg attttgtcaa
    39841 agtaccgtgg caggcgctga ccaataaagc ctatgccaaa tctattgccg atcaaattga
    39901 tatcaataaa gcgaagccat ccagcgaaat tcgccccggc aagcttgcgc cttatgagag
    39961 caatcaaact acccattact cagtggtgga taaagatggt aacgcggtgg cggtgaccta
    40021 tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta ttctgcttaa
    40081 taatcagatg gatgatttct ccgctaaacc gggcgtaccg aacgtttacg ggctggtggg
    40141 cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcgtcaatgt caccaacgat
    40201 tgtggtgaaa gacggtaaaa cctggctggt caccggtagc cctggcggga gccggatcat
    40261 cactacagtg ctgcaaatgg tggtgaatag catcgattat ggcatgaacg tcgccgaagc
    40321 gaccaatgcg ccgcgtttcc accatcagtg gttgccggac gagctgcgtg tcgaaaaagg
    40381 gtttagcccg gatacgctca agctgctgga agcaaaaggt cagaaagtgg cgctgaaaga
    40441 ggcgatgggc agtacacaaa gcattatggt tgggccggac ggtgagttgt acggcgcatc
    40501 cgacccgcgc tcggtggatg atttaacggc ggggtactaa ggttagcggc cctcttcgtg
    40561 ggaagagggc tattttgtca gggcaagccg aaggtagcct tttttatttc gtaatcctgt
    40621 agatattctt ccagcggctt tgccgttccc agagaatgaa tttcaccact gtctttatca
    40681 ataataaaag gtgcgttacc agctaagcgc gcggcctcat ctccagtttc gagaaattct
    40741 cgtgcttcga aacagaaata ccagccctgg ctaaagcgtc catgtagagt aatgacgacc
    40801 gggagatctg catcatcaag gtaatggttc gctttcgcga atgcgtcgtg ataagtaatc
    40861 ataattataa taaatattat tgttgagtgt tatattatta ttcctgtgtg atacattgag
    40921 caaagacgcg ttcatcttcg tcaaagatat tattaaatgc tggttttgaa aaaagttcat
    40981 cggataagat atagtcacga aataagtatc tgctaatatt ttatgatttc tttttcagaa
    41041 cgtcgaacgg gattttctta tttaagatat cagaatctta tgattcgggt aaaattctaa
    41101 ataaatcaac atgtcattaa aatcatcata gccattagct atttcggcta aaatagagac
    41161 tacatgtctt cggtccatct cacttaagga gtgtagttcc gttgtaagtt tttccatagc
    41221 ttgcactgct aaatttcgaa caaggaattt tctgctggta atctctaaaa agatggcatg
    41281 gtttacaatg atttttgttt ccttttgatt attatgaaca actgtccatg atttcgttta
    41341 agaatgaaga gaaatcacta aacgaactga atatattttc tgtgccaata ttatctctaa
    41401 tttcaaaaaa gttactttta atgtcataag taaatattga aatgctatca tccccgatca
    41461 ctaaccgttc ttgtagatca gggtttatga aatcatcatt atttctataa aattcattta
    41521 cggcaaaaag gtttttaact gaaggttctg gaatcgagag gctaaataat cgtaatccat
    41581 taacttcgaa tccatccatt gctcttaaaa aagtaagata atcaggttga ttcttaaaaa
    41641 gaatactaac ttcattacat aacttctcca ggcaattagt tgaattagaa ttaaattcta
    41701 tttggattat actttcaata aatgattcct tgagcggtgc ttcaaatgga tagtctaaat
    41761 cattcatcat ttttattaat ttatttattt gggttttagt catcattttg tgtggtccat
    41821 aat
//
LOCUS       DAJTMK010000013        40046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTMK010000013 DAJTMK010000000
VERSION     DAJTMK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40046
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.013"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="OO135_000597"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="OO135_000597"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5396554.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OO135_000598"
                     /pseudo
     CDS             complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OO135_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2717..2907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3021..4121
                     /gene="trmA"
                     /locus_tag="OO135_000599"
     CDS             3021..4121
                     /gene="trmA"
                     /locus_tag="OO135_000599"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5396555.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4161..4520)
                     /locus_tag="OO135_000600"
     CDS             complement(4161..4520)
                     /locus_tag="OO135_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5396556.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OO135_000601"
     CDS             complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OO135_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5396557.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5491..6891
                     /gene="sthA"
                     /locus_tag="OO135_000602"
     CDS             5491..6891
                     /gene="sthA"
                     /locus_tag="OO135_000602"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5396558.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OO135_000603"
     CDS             complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OO135_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5396559.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8056..9429)
                     /gene="argH"
                     /locus_tag="OO135_000604"
     CDS             complement(8056..9429)
                     /gene="argH"
                     /locus_tag="OO135_000604"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5396560.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDSLKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9490..10266)
                     /gene="argB"
                     /locus_tag="OO135_000605"
     CDS             complement(9490..10266)
                     /gene="argB"
                     /locus_tag="OO135_000605"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5396561.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLMLLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10274..11278)
                     /gene="argC"
                     /locus_tag="OO135_000606"
     CDS             complement(10274..11278)
                     /gene="argC"
                     /locus_tag="OO135_000606"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5396562.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11432..12583
                     /gene="argE"
                     /locus_tag="OO135_000607"
     CDS             11432..12583
                     /gene="argE"
                     /locus_tag="OO135_000607"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5396563.1"
                     /translation="MKNKLPPFIDIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLTSIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKESYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12935..15586
                     /gene="ppc"
                     /locus_tag="OO135_000608"
     CDS             12935..15586
                     /gene="ppc"
                     /locus_tag="OO135_000608"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5396564.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTITGIAAGMRNTG"
     gene            15769..17502
                     /locus_tag="OO135_000609"
     CDS             15769..17502
                     /locus_tag="OO135_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5396565.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRHVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17717..18568
                     /locus_tag="OO135_000610"
     CDS             17717..18568
                     /locus_tag="OO135_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5396566.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLI
                     EKPTTPE"
     gene            complement(18555..18896)
                     /locus_tag="OO135_000611"
     CDS             complement(18555..18896)
                     /locus_tag="OO135_000611"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5396567.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVDLLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18898..19776)
                     /locus_tag="OO135_000612"
     CDS             complement(18898..19776)
                     /locus_tag="OO135_000612"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5396568.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQCYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLISG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19742..22039)
                     /locus_tag="OO135_000613"
     CDS             complement(19742..22039)
                     /locus_tag="OO135_000613"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5396569.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22090..22410)
                     /locus_tag="OO135_000614"
     CDS             complement(22090..22410)
                     /locus_tag="OO135_000614"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5396570.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22425..23504)
                     /locus_tag="OO135_000615"
     CDS             complement(22425..23504)
                     /locus_tag="OO135_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5396571.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23813..26314
                     /gene="ptsP"
                     /locus_tag="OO135_000616"
     CDS             23813..26314
                     /gene="ptsP"
                     /locus_tag="OO135_000616"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5396572.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     IQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26326..26988
                     /gene="fsa"
                     /locus_tag="OO135_000617"
     CDS             26326..26988
                     /gene="fsa"
                     /locus_tag="OO135_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5396573.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            26999..28102
                     /gene="gldA"
                     /locus_tag="OO135_000618"
     CDS             26999..28102
                     /gene="gldA"
                     /locus_tag="OO135_000618"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR5396574.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWFVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28376..28993
                     /locus_tag="OO135_000619"
     CDS             28376..28993
                     /locus_tag="OO135_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5396575.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29020..29925)
                     /gene="yijE"
                     /locus_tag="OO135_000620"
     CDS             complement(29020..29925)
                     /gene="yijE"
                     /locus_tag="OO135_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5396576.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGIHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVALL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30019..32198)
                     /gene="katG"
                     /locus_tag="OO135_000621"
                     /pseudo
     CDS             complement(30019..32198)
                     /gene="katG"
                     /locus_tag="OO135_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
     gene            complement(32527..33417)
                     /gene="metF"
                     /locus_tag="OO135_000622"
     CDS             complement(32527..33417)
                     /gene="metF"
                     /locus_tag="OO135_000622"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5396577.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33766..36198)
                     /gene="metL"
                     /locus_tag="OO135_000623"
     CDS             complement(33766..36198)
                     /gene="metL"
                     /locus_tag="OO135_000623"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5396578.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLIRAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDGFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36201..37361)
                     /gene="metB"
                     /locus_tag="OO135_000624"
     CDS             complement(36201..37361)
                     /gene="metB"
                     /locus_tag="OO135_000624"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5396579.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRIVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLSENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37638..37955
                     /gene="metJ"
                     /locus_tag="OO135_000625"
     CDS             37638..37955
                     /gene="metJ"
                     /locus_tag="OO135_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5396580.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38139..38747
                     /locus_tag="OO135_000626"
     CDS             38139..38747
                     /locus_tag="OO135_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5396581.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38892..39567
                     /locus_tag="OO135_000627"
                     /pseudo
     CDS             38892..39567
                     /locus_tag="OO135_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39739..40011)
                     /locus_tag="OO135_000628"
     CDS             complement(39739..40011)
                     /locus_tag="OO135_000628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396582.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAVLYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
BASE COUNT         9848 a        11041 c         9999 g         9158 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacta gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccactactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acgggtatcg agcaacgatg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaccatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcacgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2341 gccagattca gccgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2401 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2461 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2521 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2581 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2641 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2701 ttcatcaata ttacgcgatg atgagacgtt ggccggcagg tcttcgggct tggaggggta
     2761 tctaagatac taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca
     2821 tcgcaccttt ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact
     2881 cacaggaccg gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac
     2941 aaataggaat tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact
     3001 ttaatttttc aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc
     3061 agttagccga aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc
     3121 cggaagtgtt tcgctcgccg gtcagtcatt accggatgcg tgcggagttc cgcatctggc
     3181 acgatggcga tgacctgtat cacatcattt tcgatcaaca aaccaaaagc cgtatccgcg
     3241 tggatagctt ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg
     3301 gtgtgcgtaa taatcccgtt ctgcgccaca agttgttcca gattgattac ctcaccacgc
     3361 tgagtaatca ggcggtggtt tccctgctat accataagaa gctggatgat gagtggcgtc
     3421 aggaagcgga ggccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg
     3481 gtcgggcaac gaaaaccaaa atcgagctgg atcaagatta catcgacgag cgtctgccgg
     3541 tcgcagggaa agagatgatc taccgtcagg tagaaaacag ctttacccag ccgaacgcgg
     3601 cgatgaatat tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt
     3661 tactggagct gtactgcggc aacggtaact tttcattagc gctggcgcgc aattttgatc
     3721 gggtattagc caccgaaatc gctaagccgt cggttgctgc tgcgcaatac aacatcgcag
     3781 ctaaccatat tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga
     3841 tgaatggtgt gcgcgagttt aaccgcctgc aagggatcga cttaaagagt tatcagtgcg
     3901 aaaccatttt tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc
     3961 aggcgtatcc gtgtattttg tacatctcct gtaacccgga aacgttatgc aagaatctgg
     4021 aaacattaag ccagacgcac aaggtcgaac gtctggctct gtttgatcag ttcccctaca
     4081 cgcaccatat ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgcc
     4141 agcatccggc ataataccga ttactcagca acttcctgct tacggttacg catcttcgcg
     4201 ccaatccaga acaccataat gacggagagt actgccggga agaagttaga gccgatatcc
     4261 ggatattccg cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaggcagct
     4321 gccagacccg gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa
     4381 accgtcagca ccagggaaat aatcgggaat acagaaaatg gcacaatgga gctaaacagc
     4441 gctgcgaaag taccattaat cgataagcca gcaactaacg ccagcagcag cgtacctcta
     4501 tcttgatttg cttgtttcat tactcgtcct tcacatttcc cggaataatt gcggttttct
     4561 cttgttcacg gcgataccag taataagccc ctttcgaaat cattcgcagt tgcagtacca
     4621 gtcgctcttc taattgccga cgttgttcga cgccgacgtc caacgcctcg gcacccgcac
     4681 tgaagacaat tgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt
     4741 tttcgagttc cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg
     4801 cggcacgaaa cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag
     4861 gattattacc gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt
     4921 tggcgatacg ctgacgcgcc tggcgcatga gttggcgtag cattaaaccg ctctcatcaa
     4981 ccatggtcag acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa
     5041 tgcccgcttc acgcgccact tcacgcaaac tcaggctggc gaagctgcgt tcagcactta
     5101 attggctaaa tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta
     5161 tgcccatcac gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga
     5221 acatactgga ttgctccagt attgtgaatg aacggtaacg caataaaaca tatttactgg
     5281 ccggttcttt tacgtacagc ggaaacctgc cgcttaaacg gagagtatcg tcgataaaaa
     5341 tccaataaaa cgtcagggca aaagtaagaa acagacaaag caaaggccgc tcaggatata
     5401 gccagataaa tgacggggat caattggctt acccgcgata aaatgttacc attctgttgc
     5461 ttttatgtat aagaacaggt aagccctacc atgccacatt cctacgatta cgatgccata
     5521 gtaataggtt ccggccccgg cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg
     5581 cgcgtcgcag ttatcgagcg ttatcaaaat gttggcggcg gttgcaccca ctggggcacc
     5641 atcccgtcga aagctctccg tcacgccgtc agccgcatta tagaattcaa tcaaaaccca
     5701 ctatacagcg accattcccg actgctccgc tcttcttttg ccgatatcct taaccatgcc
     5761 gataacgtga ttaatcaaca aacgcgcatg cgtcagggat tttacgaacg taatcactgt
     5821 gaaatattgc aggggaacgc tcgctttgtt gacgagcata cgttggtgct ggattgcccg
     5881 gacggcagcg tcgaaacact aaccgctgaa aaatttgtta ttgcctgcgg ctctcgtcca
     5941 tatcatccaa cagatgttga tttcacccat ccacgcattt acgacagcga ctcaattctt
     6001 agcatgcacc acgaaccgcg ccatgtactt atctatggtg ctggagtgat cggctgtgaa
     6061 tatgcgtcga tcttccgcgg tatggatgta aaagtggatc tgatcaacac ccgcgatcgc
     6121 ctgctggcat ttctcgatca agagatgtca gattctctct cctatcactt ctggaacagt
     6181 ggcgtagtga ttcgtcacaa cgaagagtac gagaagatcg aaggctgtga cgatggtgtg
     6241 atcatgcatt tgaagtcggg taaaaaactg aaagctgact gcctgctcta tgccaacggt
     6301 cgcaccggta ataccgattc gctggcgtta cagaacattg ggctggaaac tgacagccgc
     6361 ggacagctga aggtcaacag catgtatcag accgcacagc cgcacgttta cgcggtgggc
     6421 gacgtgattg gttatccgag cctggcatca gcggcctatg accaggggcg cattgccgcg
     6481 caggcgctgg taaaaggcga agccaccgca catctgattg aagatatccc taccggcatt
     6541 tacaccatcc cggaaatcag ctctgtgggc aaaaccgaac agcagctgac cgcaatgaaa
     6601 gtgccatatg aagtgggccg cgcccagttt aaacatctgg cacgcgcaca aatcgtcggc
     6661 atgaacgtgg gcacgctgaa aattttgttc catcgggaaa caaaagagat tctgggtatt
     6721 cactgctttg gcgagcgcgc tgccgaaatt attcatatcg gtcaggcgat tatggaacag
     6781 aaaggtggcg gcaacactat tgagtacttc gtcaacacca cctttaacta cccgacgatg
     6841 gcagaagcct atcgggtagc tgcgctaaat ggcttaaacc gcctgtttta acactttatc
     6901 gaaatggcca tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag
     6961 cggtgagcca ggacgataaa ccaggccaat cgtgcggcga ggttccggct taatacacgg
     7021 caaataaaca accccatcgc gtttgcgctc cggcggcaca gccagcgcag gcagtaaagt
     7081 gatcccgcta cctgccgcca ccatgttacg cagtgtctcc aggctggtcg cgcggaagtg
     7141 tgtatcttca tccgccccgg cttcaaagca gaaacccatt gcctgatcgc gcaaacagtg
     7201 accatcttcc agcatcagca gtttttcccc tgccagatcg gccatcggta cgcattcgcg
     7261 gttcgcccac gggtgatctt catagatagc cagcaacatt ggctcatcaa acaacggcac
     7321 ttcaatgaat gcttcgctct ctttcaccag cgcgaggatc acgcaatcga gtttgccgct
     7381 gtccagttgc gccagtaact ggtgggtctg tgcttcatgc agatacattt ccagctttgg
     7441 aaatgtctgg tgcagcatcg ggataatatg cggcagcagg tacggtccaa ccgtaggaat
     7501 caaaccaata tgcagcggtc cggacatcgt ctcgccctgc tggcttgcca tctctttaag
     7561 gactttcacc tcacgcagca cggtacgcgc ctgatccacc agcagcattc ccgcctgggt
     7621 gaacaacact ttacggctgg tccgctccag caacatcacg cccagctcat cttccagctt
     7681 acgaatttgg ccgctaagcg tcggctggct aacgtggcag gaatctgccg cacgccgaaa
     7741 atggcggtgt tcagccaatg ccaccaggta ctcaagatca cgaatattca ttatccatcc
     7801 tccatcgcca cgatagttca tggcgatagg tagaatagca atgaacgatt atccctatca
     7861 agcattctga ctgataattg ctcacagaaa cggagcggca cctcttttaa cccttgaagt
     7921 cactgcccgt ttcgagagtt tctcaactcg aataactaaa gccaacgtga acttttgcgg
     7981 atctccagga tccgcttttt tgccataaaa aagcccggcg ataagccagg ctcaaatttg
     8041 tacatataaa tgttcttacc ccaaccgcgc ctgcgcaaaa gcaatcgctt gggccacctg
     8101 ctgcggtgag acgccgcctt ttgccgcacg cttgtcgagg cacgattgta gcgacagaat
     8161 cggatagaca tcttcgccaa tcacgtggct gaatttctgc aactcgtcga gcggcagatc
     8221 ttccagcggt ttgccctgac gaatggcttc caccaccgct tcacccacga tatggtgcgc
     8281 ctcgcggaaa ggtacgcctt tcgccaccag gtaatccgcc agttcggtgg cgttggcgta
     8341 accctgctga gccgcttcct ggcaacgtgg acgtttcacc tgaatgccgt ccagcaccag
     8401 cgccgccata tgcaggcagt ccagccaggt atcgagcgcg tcgaacagac cttctttgtc
     8461 ttcctgcata tctttgttgt aagccagcgg caaacctttc agcgtcatca tcatgccggt
     8521 taacgcaccc tgcacccgac cgcatttacc gcgaatcagc tccagcgcat ccgggttttt
     8581 cttctgcggc attaatgatg aaccggaagt cacgcggtca gaaagctcca caaaccccgc
     8641 ttcgccggtg ttaaagaaaa tcagatcttc agcaaaacgc gacagatgca ccatgccgat
     8701 agcggcagcc gacagcagtt ccaacacatg gtcacggtca gaaacgctgt ccagactgtt
     8761 acgggtcgcc gaagcaaagc ccagccagcc tgctaactgt tcacggtcga tttcataggc
     8821 cgttcccgcc agcgcgccac agcctaatgg gctgacatcc agacgcttaa gcgaatcctg
     8881 caaacggctt tcatcacgcg ccagcatctc aacataggcc aggcaccagt gcgcgaacgt
     8941 caccggctgg gcgcgttgca ggtgagtgta acctggcatt accgcgtcct gattgttttg
     9001 tgcggtttcc accagcgccg attgcagctg ccggttagcc gtcagtaact cgctaacggt
     9061 atctttgcac cacagtttca ggtcagtcgc tacctgatca ttacggctac gcccggtatg
     9121 cagctttttg cctaactggc ccactttgtc gatcagtttg ccttccaccc agctatggat
     9181 atcttcggcg tcgctttcaa ggatttgttg tggcctagcg cgaacatctt ccagcaacac
     9241 gttcagcgcc tcttccagtt gcgcctgctc ttctgcggtt aacacgccga ccgttaccag
     9301 ggctttggac caggccacag agccaacaat atcctgctcc gccagacggt aatcaaagcg
     9361 cagtgagtcg ttgaattgtt tgaaccgttg atctgctgcc tgggtaaaac gcccgcccca
     9421 aagtgccata actctgtttc cttattttga aattcaatgc cggaaagctg cgcctccggc
     9481 taacaaaact taagctaaaa tccgcgtacc catcggcata ccgttaaaca gtgccggaag
     9541 ctgctccgca tgacgccagg aggcgatatc taccggacgg cccagcgtgc gggccgcatc
     9601 cagcgccgcg ttcactttca ctatcatgcc gtcagtaata atgccctgct caatcagttg
     9661 ttctgctttc gcggcggtca tttcggcaat gcgttgccct ttgccgtcga gaatgccgct
     9721 gacgtcggag agcaacatca gatccgcgcc cagcgttgcc gccagcgccg ttgccgcctg
     9781 gtcggcattg acgttcatca gttgcccttc gtctgttacg ccaatggagc tgaccaccgg
     9841 cagataaccg ttctccagca aggagttgat aagcttaggc gaacctggct gcgccagtcc
     9901 aacatgacct aactcttcat caagctgggt cactttgacg ctgtcgccat caccgagaaa
     9961 caaacctacg gccgcaatct gatgtttctt cgcccatgcc aacagggttt tatttgccgt
    10021 tcccgccagt gctccggtga taatgtctat ctgatcagca ggcgtcaccc gcaggccgtt
    10081 tttctttttc accggcagat tcagcccttt catcagctca tccaccacgc aaccgccgcc
    10141 gtgcacaatc accagcggac gctgatgtga ctcacgataa ttcaccagtg cgctaaacag
    10201 acgttccagc gcctcttcac tatccagcag tacgccgccc agtttgataa ttaatggatt
    10261 catcattgca cccttaaata agagactgcg tttccgcata gccgaaacga atattggcgc
    10321 actgtaccgc ttgtgccgcc gcgcctttca gtaagttgtc ttcggtcgcc acaataatca
    10381 gatgctcgcc ctgaacggca aacccgatat cgcaaaatgg cagcccaacg acatttttca
    10441 gcgccggaac gcctttgtca tacagccgca ccagcggttt atgggcatac gcctgctgta
    10501 acacttgcgc gacttgcgcc tgggtcacac ccgatttcag gcggcaggta atggtttcga
    10561 gaatgccgcg cgggaaattg cccagatgtg gggtgaagat aacgtcagca ccgaggtgtg
    10621 tggcgatctc tggttgatgg cgatgagtaa agacgccata cggttgcagg ctaacttcac
    10681 aaaaactatt tgaaatcgct gccttacgtc ctgcaccgct cacgccgctg gtggcgttga
    10741 tcgccggcca ctgattgagg tcaagaagat cggcatcaat caacggtttc agcgccagct
    10801 gtgccgccgt cggataacag cctggcaccg caatcaaatt cgcttctttt aatttattac
    10861 cgcaccactc cgccagaccg taggctgcct gttccagcag ttccgggtat tgatgggtaa
    10921 agccgtaata tttttcatag aaggtggcgt cgttaacacg aaacgcgccg gaaaggtcga
    10981 acaccacgca gcccgcttca agaaactgcg gcgctaaatc gtggctaact tcatgggcgg
    11041 tggcgagaaa cactacgtcc acccctgggc taaactcgct gatatccgac atcggctgca
    11101 acggcagctc aacgatgcct tttagctgcg gatgcaaatc ggagattaac tttcccgcat
    11161 cattgctttg cgctgaaaca gtcaaagcgg ttatgttcat atgcggatgg cgatttacat
    11221 aggtcactag ctctgcgcca gcgtagccgc tggcacccac aatcagcgta ttcaacatcg
    11281 gggctattca ccttcttatg tctggttgcc aggttaaacg taaaacattc accttacggc
    11341 tggtgggttt tattacgctc aacgttagtg tatttttatt cataaatact gcatgaatat
    11401 tgatactatc atgaccagag gtgtgtcaac aatgaaaaac aaattaccgc catttatcga
    11461 tatttaccgc gctctgattg ccacaccttc aataagcgct acggaagagg cactcgatca
    11521 aagcaatgca gatttaatca ctctgctggc ggactggttt aaagatttgg gcttcaatgt
    11581 ggaagtgcag cctgttccag gaactcgcaa caaattcaat atgctgacaa gtatcggaca
    11641 gggggctggc ggcttgttgc tggcggggca taccgatacg gtgccatttg atgacggtcg
    11701 ctggacgcgc gatccgttta cactgacgga gcatgacggc aagctttacg gcttaggcac
    11761 cgccgacatg aaaggctttt ttgcgtttat ccttgatgcg ctacgcgatg tcgacgtcac
    11821 gaaactgaaa aaaccgctct acattctggc gactgctgat gaagaaacca gtatggccgg
    11881 agcgcgttat tttgccgaaa ctaccgccct gcgcccggat tgcgccatca ttggcgaacc
    11941 gacgtcacta caaccggtac gcgcacataa aggtcatatc tctaacgcca tccgtattca
    12001 gggccagtcg gggcactcca gcgatccagc acgcggagtt aacgctatcg aactaatgca
    12061 cgacgccatc gggcatattt tgcaattgcg cgataacctg aaagaaagtt atcactacga
    12121 agcatttacc gtgccatacc ctacgctcaa cctcgggcat attcacggtg gcgacgcttc
    12181 taaccgtatt tgcgcttgct gtgagttgca tatggatatt cgtccgctgc ctggcatgac
    12241 actcaatgaa cttaatggtt tgctcaacga tgcattggct ccggtgagcg aacgctggcc
    12301 gggtcgtctg acggtcgacg agctgcatcc gccgatccct ggctatgaat gcccaccgaa
    12361 tcatcaactg gttgaagtgg ttgagaaatt gctcggagca aaaaccgaag tggtgaatta
    12421 ctgtaccgaa gcgccgttta ttcaaacgtt atgcccgacg ctggtgctcg gtccgggttc
    12481 catcaatcaa gctcatcagc ctgatgaata tctggagacg cggtttatta aacccacccg
    12541 tgagttgatt atccaggtaa ttcaccattt ttgctggcat taaaacgtag gccggataag
    12601 gcgctcgcgc cgcatccggc accgttgcca aactccggtg ccgcaataat gtcggatgcg
    12661 atgcttgcgc atcttatccg acctacacct ttggtgttat gtatggcgat tttttaacat
    12721 ttccataagt tacgcttatt taaagcgtcg tgaatttaat ggcgtaaatt cctgctattt
    12781 attcgtttgc tgaagcgatt tcgcagcatt tgacgtcacc gcttttacgt ggctttataa
    12841 aagacgacga aaagcaaagc ccgagcatat tcgcgccaat gcgacgtgaa ggatacaggg
    12901 ctatcaaacg atcagatggg gtgtctgggg taatatgaac gaacaatatt ccgcattgcg
    12961 tagtaatgtc agtatgctcg gcaaagtgct gggagaaacc atcaaggatg cgttgggaga
    13021 acacattctt gaacgcgtag aaactatccg taagttgtcc aaatcttcac gcgctggcaa
    13081 tgatgctaac cgccaggagt tactcaccac cttacaaaat ttgtcgaacg acgagctgct
    13141 gcccgttgcg cgtgcgttta gtcagttcct gaacctggcc aacaccgccg agcaatacca
    13201 cagcatttcg ccgaaaggcg aagctgccag caacccggaa gtgatcgccc gcaccctgcg
    13261 taaactgaaa aaccagccgg aactgagcga agacaccatc aaaaaagcag tggaatcgct
    13321 gtcgctggaa ctggtcctca cggctcaccc taccgaaatt acccgtcgta cactgatcca
    13381 caaaatggtg gaagtgaacg cctgtttaaa acagctcgat aacaaagata tcgctgacta
    13441 cgaacacaac cagctgatgc gtcgcctgcg ccagttaatc gcccagtcat ggcataccga
    13501 tgaaatccgt aagctgcgtc caagcccggt agatgaagcc aaatggggct ttgccgtagt
    13561 ggaaaacagc ctgtggcaag gcgtaccaaa ttacctgcgc gaactgaacg aacaactgga
    13621 agagaacctc ggctacaaac tgcccgtcga atttgttccg gtccgtttta cttcgtggat
    13681 gggcggcgac cgcgacggca acccgaacgt cactgccgat atcacccgcc acgtcctgct
    13741 actcagccgc tggaaagcca ccgatttgtt cctgaaagat attcaggtgc tggtttctga
    13801 actgtcgatg gttgaagcga cccctgaact gctggcgctg gttggcgaag aaggtgccgc
    13861 agaaccgtat cgctatctga tgaaaaacct gcgttctcgc ctgatggcga cacaggcatg
    13921 gctggaagcg cgcctgaaag gcgaagaact gccaaaacca gaaggcctgc tgacacaaaa
    13981 cgaagaactg tgggaaccgc tctacgcttg ctaccagtca cttcaggcgt gtggcatggg
    14041 tattatcgcc aacggcgatc tgctcgacac cctgcgccgc gtgaaatgtt tcggcgtacc
    14101 gctggtccgt attgatatcc gtcaggagag cacccgccat accgaagcgc tgggcgagct
    14161 gacccgctac ctcggtatcg gcgactacga aagctggtca gaggccgaca aacaggcgtt
    14221 cctgatccgc gaactgaact ccaaacgtcc gcttctgccg cgcaactggc aaccaagcgc
    14281 cgaaacgcgc gaagtgctcg atacctgcca ggtgattgcc gaagcgccgc aaggctccat
    14341 tgccgcctac gtgatctcga tggcgaaaac gccgtccgac gtactggctg tccacctgct
    14401 gctgaaagaa gcgggtatcg ggtttgcgat gccggttgct ccgctgtttg aaaccctcga
    14461 tgacctgaac aacgccaacg atgtcatgac ccagctgctc aatatcgact ggtatcgcgg
    14521 cctgattcag ggcaaacaga tggtgatgat tggctattcc gactcagcaa aagatgcggg
    14581 cgtgatggca gcgtcctggg cgcaatatca ggcacaggat gcattaatca aaacctgcga
    14641 aaaagcgggt attgagctga cgttgttcca cggtcgcggc ggttccattg gtcgcggcgg
    14701 cgcacctgcc catgcggcac tgctgtcaca accgccagga agcctgaaag gcggcctgcg
    14761 cgtaaccgaa cagggcgaga tgatccgctt taaatatggt ctgccagaaa tcaccgtcag
    14821 cagcctgtcg ctttataccg gggcgattct ggaagccaac ctgctgccac cgccggagcc
    14881 gaaagagagc tggcgtcgca ttatggatga actgtcagtc atctcctgcg atgtctaccg
    14941 cggctacgta cgtgaaaaca aagattttgt gccttacttc cgctccgcta cgccggaaca
    15001 agaactgggc aaactgccgt tgggttcacg tccggcgaaa cgtcgcccaa ccggcggcgt
    15061 cgagtcacta cgcgccattc cgtggatctt cgcctggacg caaaaccgtc tgatgctccc
    15121 cgcctggctg ggtgcaggta cggcgctgca aaaagtggtc gaagacggca aacagagcga
    15181 gctggaggct atgtgccgcg attggccatt cttctcgacg cgtctcggca tgctggagat
    15241 ggtcttcgcc aaagcagacc tgtggctggc ggaatactat gaccaacgcc tggtagacaa
    15301 agcactgtgg ccgttaggta aagagttacg caatctgcaa gaagaagaca tcaaagtggt
    15361 gctggcgatt gccaacgatt cccatctgat ggccgatctg ccgtggattg cagagtctat
    15421 tcagctacgg aatatttaca ccgacccgct gaacgtattg caggccgagt tgctgcaccg
    15481 ctcccgccag gcagaaaaag aaggccagga accggatcct cgcgtcgaac aagcgttaat
    15541 ggtcactatt accgggattg cggcaggtat gcgtaatacc ggctaatctt cctcttctgc
    15601 aaaccctcgt gcttttgcgc gagggttttc tgaaatactt ctgttctaac accctcgttt
    15661 tcaatatatt tctgtctgca ttttattcaa attctgaata taccttcaga tatccttaag
    15721 gaattgtcgt tacattcggc gatatttttt tcaagacagg ttcttactat gcattccaca
    15781 gaagtccagg ctaaacccct ttttagctgg aaagccctgg gttgggcact gctctacttt
    15841 tggtttttct ctactctgct acaggccatt atttacatca gtggttatag tggcaccaac
    15901 ggcattcgcg actcgctgtt attcagctcg ctgtggttga tcccggtatt cctctttccg
    15961 aagcggatta aaattattgc cgcagtaatc ggcgtggtgc tatgggcggc ctctctggcg
    16021 gcgctatgct actacgtcat ctacgggcag gagttctcgc agagcgttct gtttgtgatg
    16081 ttcgaaacca acaccaacga agccagcgag tatttaagcc agtatttcag cctgaaaatt
    16141 gtgcttatcg cgctggccta tacggcggtg gcagttctgc tgtggacacg cctgcgcccg
    16201 gtctatattc caaagccgtg gcgtcatgtt gtctcttttg ccctgcttta tggcttgatt
    16261 ctgcatccga tcgccatgaa tacgtttatc aaaaacaagc cgtttgagaa aacgttggat
    16321 aacctggcct cgcgtatgga gcctgccgca ccgtggcaat tcctgaccgg ctattatcag
    16381 tatcgtcagc aactaaactc gctaacaaag ttactgaatg aaaataatgc cttgccgcca
    16441 ctggctaatt tcaaagatga atcgggtaac gaaccgcgca ctttagtgct ggtgattggc
    16501 gagtcgaccc agcgcggacg catgagtctg tacggttatc cgcgtgaaac cacgccggag
    16561 ctggatgcgc tgcataaaac cgatccgaat ctgaccgtgt ttaataacgt agttacgtct
    16621 cgtccgtaca ccattgaaat cctgcaacag gcgctgacct ttgccaatga aaagaacccg
    16681 gatctgtatc tgacgcagcc gtcgctgatg aacatgatga aacaggcggg ttataaaacc
    16741 ttctggatca ccaaccagca gacgatgacc gcccgcaata ccatgctgac ggtattttcg
    16801 cgccagaccg acaagcagta ctacatgaac cagcaacgta cgcagagtgc gcgtgaatac
    16861 gacaccaacg tgctgaagcc gttccaggat gtgctgaatg accctgcgcc gaagaaactg
    16921 atcatcgttc atctgctggg tacgcatatc aaatacaaat accgctaccc ggaaaatcag
    16981 ggcaagtttg atggcaatac cgatcatgtt ccgccaggat taagcgcaga agagctggaa
    17041 tcatataacg attatgacaa cgctaacttg tataacgatc atgtggttgc cagcctgatt
    17101 aaagacttta aagcggcaga cccgaacgga ttcctggttt acttctctga ccacggtgaa
    17161 gaggtttacg acacgccgcc gcacaaaact caggggcgta acgaagacaa cccgacgcgc
    17221 cacatgtaca ccattccgtt cctgctgtgg acgtcggaaa aatggcaagc gactcatccc
    17281 cgtgatttct cacaggatgt cgatcgtaaa tacagcctgg cggaactgat ccacacctgg
    17341 tcagatttgg cgggcttatc ttacgacggt tacgatccaa cccgttcagt ggtgaatccg
    17401 cagttcaaag aaactacccg ctggattggt aacccgtaca agaaaaacgc gctgatcgat
    17461 tacgacactc tgccgtatgg cgaccaggta ggtaatcagt aattatcagc caggcgctat
    17521 ttgccggatg cgacgcttga cgcgtcttat ccggcctaca gatcgcggcg gtaggctgga
    17581 taaggcgttt acgccacatc cggcaataca actgccgcaa tgtttacatc gaactcccct
    17641 caggccacac ttttcaggtg gcctttttta ataatgccag ttactgtttt cccgtctaca
    17701 tacctaaggg aataacatgt atcacgacat cagctatctg ctctcccgcc tgatcaacgg
    17761 cccgctgtcc ctgcgccaga tttactttgc cagcagtaac ggcccggtgc ccgatcttgc
    17821 gtatcaggtc gattttccac ggctggaaat tgtgctggaa ggtgagtttg ttgataccgg
    17881 cgctggagca acgttagttc ccggcgatgt gctgtacgtt gccgctggtg gctggaattt
    17941 cccacaatgg aaaacacccg ctaccacctt tagcgtgctg tttggcaaac agcaactcgg
    18001 cttcagcgtc gtgcaatggg atggcaaaca atatcaaaat ctggcgaagc aacacgtcgc
    18061 ccgacgcggc ccacgtatag gttcttttct gctacaaacg ctcaatgaaa tgcaaatgca
    18121 gccgcaggag cagcaaacgg caaggcttat cgtagccagc ctgcttagcc actgccgcga
    18181 tttgctcggc agccaaatcc agaccgcctc acgcagccag gcactatttg aagctattcg
    18241 cgattatatc gacgaacgct atgcctccgc gcttacccgc gaatctgtcg cacaggcatt
    18301 ctatatttca ccaaactatc tctcgcacct gtttcaaaaa acgggggcca ttggttttaa
    18361 cgaatacctg aatcacacgc gactggagca cgctaagacg ttattgaaag gctatgacct
    18421 gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac agcaactact tctgccgtct
    18481 gtttcgtaaa aacaccgaac gctcgccgtc ggaatatcgc cggcagtacc acagccaact
    18541 gatagaaaaa ccgactactc cagaataaga tgggtttgct gcggcgcaga aagcactttg
    18601 cgcaccgcgc tcattacccg ctgcggctca cgcaggaatg cgtagatgct gcattgcaca
    18661 tagcggcaat gttcaaatcg ctcggcaccg gcaagctcga tatccgtaat caacagatca
    18721 acatccgccc gacggatatc ctcgtccgct aaacgattct ccgttcccag cgcgccctga
    18781 gtttcaatgc tgacgcccca cttctctaac tggcacaact tttccagccg ttccgccgcc
    18841 atataagtat gcgccacgcc gcttacgcag gcggttactg ccaccaggta tgccatttta
    18901 acctcccacg gtaacctgaa atccggcccg ttctgccatt tcgcgcatcg ttgccacatc
    18961 ggctgatgac ggcgcagcaa catccttcat cgaccatgtt ttccccagca ggcggtattt
    19021 cggctcgccg tactgatgaa acggcaacag atggatctgc ctgatattca gcgggatcag
    19081 cacgtccagc gcctgctgca tattctcccg gctgagtgtg aaaccggaga tcagcggcaa
    19141 acgcgggatc acgttgacac cctcactcac cagcaaacgc aggttctcca gtacgcgtgg
    19201 caggttcatc ttcaccacat cccgcgcctg agtcgcgtcc ataattttta aatcgaacaa
    19261 cacttcatcg cacaatttcg ccagcggtaa cagcttgctg gctggtgcgt ctccggcagt
    19321 ttcaatggca catgagacac cccacagccg cagtcgctgt aaaaaacggg tagcaaactc
    19381 cgcctgcatt aacacttcgc cgccagaaag cgtgacgccg ccgccggacg tgcgaaaaaa
    19441 aatgtcatct ttcatcactt cccgttccag agcgtcaagg ctgatatcgc gaccaatccg
    19501 ttcaaacgcc ccggaggggc attcatccgc atcacgcaaa catttcgcac agtgcagaca
    19561 tttcgcctct ctgcgtaccg tctggatttt gccggagatc gactccggat tagcacacca
    19621 cgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta cgaatgccct cgccgtcatt
    19681 cagtgaataa cactgaatgt tgaaaatgcg cgccacatca ttgcggcgcg tttccacaac
    19741 gttacagctg atgcgctgtc cggcggatga tgtcatcctg gatctccttc gacagttcga
    19801 caaagaaggc gctgtatccg gcaacgcgca ccaccagccc ggcataatct tgtgggcgct
    19861 gttgcgcttc ccacaacgtg tcggcgttca ccacgttaaa ctgaatatgc tgcaacttaa
    19921 gctgggtaaa cgcccgtaag aagtcggcca gtttgcgtaa tcccgcttca ccttccaggg
    19981 tcgccggagt gaatttcacg ttcagcaacg taccgttaga cagcagcgta ttatcgagct
    20041 tactgactga cttcagtacc gctgttggcc cttgttcgtc ctggcccagc ataggtgaca
    20101 agccgccgtc tgccagctgt tctccggcaa aacgaccgtc tggcgtcgcg ccaaccaccg
    20161 accccaacgg gacgtgagca gaaacggtat acgatcccgg cgtgaagtaa ccaccgcgcg
    20221 ggttctggta tttttccact tctttgcagt agtggcgcaa cagttcggcg ctaatgttat
    20281 ccacctcgtc gatatcgtta ccatatttct caaagcggtt aattaagcga gcgcggactt
    20341 tttcgccttc tggcgttgca aagttggctt ttaataccga cagcaattcg tcaaaactta
    20401 aacgctgttg ctcaaaaacc atccctttca acgcatggag agaatcgctc aggttggcga
    20461 taccgatccc ctgtacgccg gagaagttat aacgcgcgcc gccatcggta atatcgcgac
    20521 ctttttccag acaatcgctg ataaaagacg agagcagcgg tacaggtgcc cagtcgcgat
    20581 ggccgatatc acaaatattg ctgccctcaa ccatcagggt gatgtagtgg ctgatcttgg
    20641 cgcggatctg ctccagtaaa ccttcataag tcagcgcagc attgccttca ttttcatgca
    20701 ggcagatttc catcactttc agcagattaa acatcgcgat gtcatgcaag ccgtaggttc
    20761 tgccgggaat agataattcc acacagccca ctacggaata gtcgcgcgca tcttccagcg
    20821 aaacgccacg gttgaggaac gctggtacca ccacttcatc gttaaagatt tgcggaatac
    20881 cggtgccgag gcgaatggtt tcggcggttt tcatcaggaa tggcgtgtcg atcaaggcgt
    20941 tagtgcgcac gccgaggttc ggttgcggta attgcacgct ttgataggcg tcaaggcaaa
    21001 ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt taacccgccg agcagtgcgg
    21061 tatagccggt cgggaaacct gcgaaataac gcgcgctgct ggtggagcgc aacagcacga
    21121 tgtcgttgca tttcacccat aaagattcga gcagttcttt caggaacgcc ggatcttcgc
    21181 cctgggttaa agatgcctga tagaacggca acatatactg gtcgaagcgc cctaaagaca
    21241 gcgaactggc gttggattcg tattgcagaa tgatgttcat gtaccagaat aactggcacg
    21301 cctgccagaa cgtctgcggc ttatgttcgg cgttatggcg agagatctcc gcaatagtca
    21361 gcagttcttc gcgacgctgg gcatctgtgc agtttgccgc catcgtttcc gccagtacgg
    21421 cgtaacgcaa aatgtgtttc tgcgaggctt ccagcagtaa cagcgctgcc tgataaaagt
    21481 gattctccgg ctgttgctga caatgttgct gcatctgtgc caccaactcc cccagcccgt
    21541 gattcaacag gcgtgggtaa tcaataataa tgtgcccctg gcctttatcc gtctggttaa
    21601 tgctgaaaat ctgcgtgctg gtcgcggctt ttacttcatc cgtcatctgc ccgttgatga
    21661 aatctttcat cgaacgtttt tcccagtacg ggaacaactc ttcacgatag atacgtttat
    21721 cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg gaattgatcc agctctttca
    21781 gcagccagta agggtccatt tccggcgaca taatcccggc gcgcggtttt acggtgcggt
    21841 taccggcaat cagttcttca tcacgaatcg aaatttcaac atgttcaagg atatacgctg
    21901 ttgctttcgc ccggcgcaat atcaccggtt cgccttcggt ttgccgatgg ctggcggtat
    21961 aaagcagcgc ccgctccagc gagatttcac gggtattggc aaacagtgca gttttgaggc
    22021 gagagatacg attcgtcatg aggaactcct gtgggaaagc gaacccgccc ttgcgagcgg
    22081 gagaagaact taagcggttt gcgccaggtg agcttcaatt ttgctcataa tggcgtcagc
    22141 acgttttact gcatcgctga tgttgacgcg cacgatggtc ttgccagcaa aacgctcttc
    22201 aaacttgatg ccgatatctt tggtcagaat caccatatca gcgctcgcca catcttccgc
    22261 agtcagttca ttttccagac caattgaccc ctgggtttct actttcactt cccagccttt
    22321 cgctttcgcg gcactttcca gcgcctctgc tgccatatag gtatgggcaa cacctgaagg
    22381 acatgcggtt actgcaataa ttttcgtcat ggctcgttcc tcaattaatt aatttcaaaa
    22441 tccaaatcca ggtcgtcttc tttttcatca gtcgaagacc catttttacg cgccagactt
    22501 ttcagcacgt tcacacaaac agccgtcacc actgctccga cagccactgc tgcgatataa
    22561 cccagcttgc cttcaaccac cggcagcaca atcagaccac cccagcctgc atagcactgc
    22621 gcaccgacca gcgccgcagt tactgcacca caaactgaac cgaccatgat ggaaggaatg
    22681 acacgcagcg gatcggcagc ggcgaaagga atcgcccctt cagtaacgcc aacacagccc
    22741 atcaccagtg ccgctttgcc ggtttcgcgc tcttctgcgg agaaattttt acgaccaatc
    22801 agcgtcgcca gccccatccc cagcggcggg atacaaatac ccacggcagc gatgaccacc
    22861 acggtataaa caccctgagc aacgcaaatc agcatgaagg cataggccac tttgttaacc
    22921 ggaccgccca tatcgaacgc cagcatcaga cccatgatca ccgccagcat aacaatgctg
    22981 ccctgctgca tcccctgaag ccactgagtc aggctgttgg tcaacgcccc tacaggctcg
    23041 cccaagcccc acatcatgat gcctgcggta atcagtgtgc cgacgatagg aataatgaag
    23101 ataggcatca ccgagcgcag aactttatgc accggaattt tcttcaggta atgcaccacg
    23161 atgccgccga taatcccggc gatcagtgca ccaaagaagc ccgcaccaaa gctgttacca
    23221 acccaggcac cgatagcgca cggagccagc gcagaacgct ctgcaatgga gtaaccaatg
    23281 taagcggcga ggaaaggcac catcagcgtc aaacctgcaa cgccgatatc aaacagtttt
    23341 ttcaaattcg gatcggctac ggcatccggc actgcgcctt tgccatacaa catgacggaa
    23401 accgccagca aaataccgcc cgataccacg aagggaatca tgtgtgaaac gcccgtcatt
    23461 aaatgctgac gggtattttt caggatctgc accaactcat tcataagctg ctcccgggct
    23521 tgcgaaagcc catgtccata tttttgatgg aggaaacaat aggcaatcgc aggcggagca
    23581 aacagtggat aaaaaggcca tttactggac ttttgtaata tcagtacaaa aatgcgatcc
    23641 gcctcataac ttgcgataaa ccggaagcat gccgcaaaac ggcgtcttgt gagacgcgcc
    23701 gcaaattgac ggttacatta catttgtcca gcttttggca gatttgtcac atggcaggcc
    23761 gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc gggagagagg caatggccct
    23821 gattgtggaa tttatttgtg agctacctaa cggcgtacat gcgcgtccgg caagccacgt
    23881 tgaaacgctg tgtaatactt tttcatcaca aattgagtgg cataacctgc gcactgaccg
    23941 caagggcaac gccaaaagcg cccttgcgct gattggcacc gatacgctgg cgggcgataa
    24001 ctgccagtta ctgatttccg gggccgacga acaggaagcg caccagcgtt taagccaatg
    24061 gctgcgcgat gaattccccc actgcgatgc gccgctggcg gaagttaaat ctgacgaact
    24121 ggaaccactg ccggtttcac tgaccaatct gaatccgcaa attatccgcg cccgcaccgt
    24181 gtgcagcggt agtgcaggcg gcattctgac gccgatctct tctttagatc tcaatgcgct
    24241 gagtaatctt cccgcagcca aagacgttga cgccgagcaa tccgcactgg aaaatggcct
    24301 gacgctggta ctgaaaaaca ttgagtttcg tctgctggat agcgacggtg ctaccagcgc
    24361 gatactggaa gctcaccgat ccctggctgg cgatacatct ttacgtcagc acttactggc
    24421 aggcgtcagc gagggattaa gctgtgccga agcgattgtc gccagtgcga atcacttttg
    24481 cgaagagttc gcccgttcca gcagcagcta cctgcaagaa cgtgccctgg acgtacgcga
    24541 cgtctgcttc cagttactcc agcaaatcta cggtgagcaa cgcttcccgg caccgggcaa
    24601 actgacgcag cccgccattt gtatggctga tgaactgact cccagccaat tcctcgaact
    24661 ggataaaaat cacctcaaag gtctgttact gaaaagtggc ggcaccacct cacataccgt
    24721 aattcttgcc cgttcgttca acattccgac actggttggt gtagatattg atgcccttac
    24781 tccgtggcag catcaaacga tttatatcga cggcaacgcc ggggcgattg tggttgagcc
    24841 aggggaagcc gtggcccgtt attatcagca agaagcccgc gtacaggacg cccttcgtga
    24901 gcaacagcgt gtctggctga cccaacaagc ccgtaccgct gacggtatcc gcattgaaat
    24961 tgccgctaac atcgctcact ccgtggaagc gcaggccgca ttcggcaatg gtgcggaagg
    25021 cgttggtttg ttccgcactg aaatgctcta tatggatcgc accagtgcac cgggcgaaag
    25081 cgagctgtac aacatttttt gtcaggcgct ggagtctgcc aacggacgca gcattattgt
    25141 gcgcactatg gacattggcg gcgacaaacc cgttgattat ctgaacattc cggcagaagc
    25201 taacccgttc ctcggttatc gcgctgtgcg tatttatgaa gaatacgcct cgctgttcac
    25261 cacacaacta cggtcgatcc tgcgcgcctc cgctcacggc agcctgaaaa tcatgatccc
    25321 gatgatctcc tcaatggaag agatcttatg ggtgaaagaa aaactggcgg aagccaaaca
    25381 gcaactgcgt aacgaacaca ttccgtttga tgagaagatc cagcttggca ttatgctgga
    25441 agtaccgtcg gtgatgttca tcatcgatca atgctgcgaa gagattgatt tctttagtat
    25501 tggtagtaat gacctgacgc aatatctgct ggcagtggat cgcgataacg ctaaggttac
    25561 tcgtcactac aacagcctga atccggcatt cttgcgggcg ctcgattacg ccgtgcaggc
    25621 ggtgcatcgc cagggcaaat ggattggtct gtgcggtgag ctgggagcga aaggttccgt
    25681 gctgccgttg ctggtcggct tagggctgga tgaactcagc atgagcgcac catcaattcc
    25741 ggcggcgaaa gcgcggatgg cgcaacttga tagccgtgag tgccgcaagt tgctcaacca
    25801 ggcaatggcc tgccgtactt cgctggaagt ggaacacctg ctggcgcaat tccgcatgat
    25861 ccaacaggac gcaccgctgg tcaccgccga gtgcatcacg ctggaaagtg actggcgcag
    25921 caaagaagaa gtgctcaaag gcatgaccga taacctgctg ctggcgggcc gctgccgcta
    25981 tccgcgtaaa ctggaagccg acttatgggc gcgcgaggcc gttttctcta ccggtctggg
    26041 ctttagtttt gccattccac acagcaaatc agaacacatt gagcaatcca ccatcagcgt
    26101 ggcgcgtctg caagcgccgg tgcgctgggg cgatgatgaa gcgcaattca tcattatgtt
    26161 aaccctgaac aaacacgctg cgggcgatca gcacatgcgc attttctcgc gcctcgctcg
    26221 ccgcatcatg cacgaagaat tccgtaacgc gctggttaac gccgcctctg ccgacgctat
    26281 cgccagcctg ctgcaacatg aactggaact gtaaaaggaa acatcatgga actgtatctg
    26341 gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca tattccccat tgccggggtg
    26401 acaactaacc cgagcattat cgctgccagc aaggagtcca tctgggaagt tctgccgcgt
    26461 ctgcaaaaag cgattggtga tgagggcatt ctgtttgctc agactatgag tcgtgacgcg
    26521 cagggtatgg tggaagaagc gaagcgcctg cgcgacgcca ttccgggcat tgtggtgaaa
    26581 atcccggtga cttccgaagg tctggccgcc attaagatgc tgaaaaaaga gggtattact
    26641 acgcttggca ccgcagtgta cagtgctgca caagggttac tggccgcgct ggcaggggca
    26701 aaatacgttg ctccgtatgt taaccgcgta gatgcccagg gcggggacgg cattcgtacg
    26761 gttcaggagc tgcaagcgct gttagaaatg cacgcaccag aaagcatggt gctggcggcc
    26821 agctttaaaa cgccgcgtca ggcgctggac tgtttactgg caggatgtga atccatcacc
    26881 ctgcccttag atgtagcgca acaaatgctc aacacccctg cggtagagtc agctatagag
    26941 aagttcgaac acgactggaa tgccgcattt ggcactactc atctctaaag gagcaattat
    27001 ggaccgcatt attcaatcac cgggtaaata catccagggc gctgatgtga ttaatcgtct
    27061 gggcgaatac ctgaagccgc tggcagaacg ctggttcgtg gtgggtgaca aatttgtttt
    27121 aggttttgct caatccactg tcgagaaaag ctttaaagat gctggactgg tagtagaaat
    27181 tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgat cgtctgcgtg gcatcgcgga
    27241 gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga aaaaccctcg atactgccaa
    27301 agcactggca catttcatgg gtgttccggt agcgatcgca ccgactatcg cctctaccga
    27361 tgcaccgtgc agcgcattgt ctgttatcta caccgatgag ggtgagtttg accgctatct
    27421 actgttgcca aataacccga atatggtcat tgtcgacacc aaaatcgtcg ctggcgctcc
    27481 tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca acctggtttg aagcacgcgc
    27541 ctgttcccgt agcggcgcta ccactatggc gggcggcaag tgcacccagg cggcgctggc
    27601 actggcagaa ctgtgctaca acactctgct ggaagaaggc gaaaaagcga tgcttgccgc
    27661 cgaacagcat gtagtaaccc ctgcgctgga acgcgtgatt gaagcgaaca cctatttaag
    27721 cggtgttggt tttgaaagcg gcggactggc ggcggctcat gcggtgcata acggcctgac
    27781 cgctatcccg gacgcgcatc actattatca cggtgaaaaa gtggcattcg gtacgctgac
    27841 gcagctggta ctggaaaacg cgccggttga ggaaatcgaa accgtagctg cacttagcca
    27901 tgcggtaggt ttgccaataa ctctcgctca actggatatt aaagaagatg tcccggcgaa
    27961 aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa accatccaca acatgcctgg
    28021 cggcgcgacg ccagatcagg tttacgccac tctgctggta gccgaccagt acggtcagcg
    28081 tttcctgcaa gagtgggaat aacctactcc aaactcccgg cttgtccggg agtttgaacg
    28141 caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa tctctgcaat atattgaatt
    28201 tgcgtgcttt ttaggccaga taaggcgttc acgccgcatc cggcactctg tgccagtaat
    28261 ctgaaactcc tacgttgtcc aggagttttt tatccgcttc tggacatttt ctctacagaa
    28321 caatcgttag cccggaaggt cgaatctgtg actcttattt tcacggacga accagatgaa
    28381 agcttcactg gcgctgctca gcctgctaac ggcatttacc agccactcct taaaatctcc
    28441 cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc aaccttgcca tcgcagacgg
    28501 tgccagacag caaatcggca acacgctatt ttacgatccg gcgtatgtgc ggcttaccta
    28561 tcctggcggt gatgtcccgc aagaacgcgg tgtatgttcc gatgtggtga tccgtgcatt
    28621 gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa gatatggcga agaattttgc
    28681 cgagtacccg caaaaatgga agttaaagcg cccggacagc aacatcgatc accgtcgggt
    28741 gcctaatctg gaaacctggt ttagccgcca caacaaaaca cgccccatca gtaaaaatgc
    28801 cggtgactat caagcgggcg atattgtctc ctggcgatta gataacgggc tggcgcatat
    28861 tggggtggtg tcagatggct tcgcccgcga cggtacgcca ctggtgatac ataacattgg
    28921 cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg atggtggggc attatcgtta
    28981 ttttgttaaa taagcgatcg ccccacatga agaaaaaatt cagatgcttt ttacactgac
    29041 agcttctttt ttcttacggc tcaccagcgc cagtgccagc acaatcagca caataccgct
    29101 accttcaacg gcccccggat tctcaccgag cagccaccag gaaaagagta cgccgcaaac
    29161 gggaacggcc agcgtgctta agctggcaat actggcaggc aagtttttca atacaaacaa
    29221 ccataagctc cacgccagcg ccgtcgccag aatcgcactg taggccagcg cccagaacac
    29281 ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa gcgaccacac tcatcaccag
    29341 cgccgcatag agcatctgcc aggatgttaa cgataataaa tccacgcgcg gatgacgggt
    29401 gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta acaccggaga ggattgccag
    29461 catggcactt ttcatcgaag agaaatcgag ttgccacggc tgcaacacca aaaataagcc
    29521 gaaagcggca atcagaatcg cgaaatattg cccacgtcgc aggcgttcac cgagaaacaa
    29581 cgcggcgaaa atcaccaccc agaacggcat ggtatagctc aggagcgcca ccttccccgc
    29641 acctccgctg accaacgccc actgcgccag accaaccatc ccgcaggttt gtaacagggc
    29701 aatggctaag gtgtatttaa acggcgtcgg gtgaattccg cgaccacgta ataaaaggac
    29761 gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg gtaaagtcga aggcaccgat
    29821 gtaactggtg acttgcttca tgaaaatcca gctataactc cagataagtg tgagcacaac
    29881 caggccactg attgccagtg ggttgctctt tcctgcggca gacatgagag atccagtgtg
    29941 tagttttcct gtggacacta tacgccggta aagaaacttc agcgactgcc tgaaagatag
    30001 tcactgaaga aggtcagatt acagcaggtc gaaacggtcg aggttcatca ctttcaccca
    30061 tgccgctacg aagtctttaa caaacttctc gtgggcatcg ctgctggcgt aaacttccgc
    30121 taccgcacgc aggacagagt tagaaccaaa taccagatcc gcacggctgg ctgtgtattt
    30181 cacttcgccg gtttcgcggt cacgtccttc gaacagctct ttcgattcgt cagtcgcttt
    30241 ccactcgtaa cgcatatcca gcaagttcac gaagaagtca ttgctcaata cgccaacgcg
    30301 gtcagtgaag acgccgtttt tgctgctatc gaagttggca cccagtacac gcatcccgcc
    30361 caccagcgca gtcatttccg gcgcggtcag cgtcagttgc tgtgctttgt cgatcagcag
    30421 tgactcggtg gtggaaacgt ccagacgagc gcgatagtta cggaaaccgt cagcaattgg
    30481 ctccagcagc tcaaacatct caatgtcagt ctgatcctga cgcgcatcaa cgcgacccgg
    30541 cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg gctttctcaa caccaaccac
    30601 accagccagc acgatgatat ccgccagcga ggctttacca gactctttct ggattttctc
    30661 cagaacaggc agagcacgaa cggctgcggc gttcacatcc cagtcgcgct gcggcattaa
    30721 tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca ccacggaagg tagaagcaga
    30781 tgcccaggcc accgatacca gctcactaac agacagacca gaatccgcaa tcgcgaattt
    30841 cagatcgata atgtcctgct cggtcgggtt gtagatcggc tgcggcagcg gatcttgcca
    30901 gatcagatct tctttcggca cttccggccc gatgtagcga gatttcggcc ccatatccct
    30961 gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg aacgcctgcg gatcgttgag
    31021 gaaacgacga gagatcttct cgaactcagg atcaaaacgc agcgtcaggt cggtcaccag
    31081 cattgtcggt ttacgtttct tcgacggatc gaacggatcc gggataattt ccggtgcgtc
    31141 taccgcttcg aactggattg ctccagccgg actgcgggtc tgtacccact catatttgaa
    31201 caggttctcg aagaaaagtt gctccactgg gtcggcgtct gggtccagac aacttccaga
    31261 ccagaggtaa tggcatctgc gccaacgccg ctgccgtaag tgctcgccca gcctaaacct
    31321 tgttcttcaa tcggtgcagc ttctggatca ggacctacat ttgatgtcgg accggcaccg
    31381 tgagttttac ccagcgtatg accacccgca atcagcgcca cggtttcttc gtcgttcatg
    31441 cccatgttgc cgaaggtcgc acggatagct gctgccgcag aaagcggttc gccgctgtga
    31501 tccgggcctt ccgggttaac gtaaatcaga cccatctcgg ttgcacccag cggtgctttc
    31561 gccagcgctt ccggatgacg gtgagtcagc caggcttttt catcacccca gttaacatcc
    31621 agatccggtt cccagacgtc ttcacgaccg gcaccaaaac cgaaggtacg gaagccggag
    31681 ttttccagcg ccacgttacc tgcgaggata aacaggtcgg cccaggagat tttctgacca
    31741 tatttctgtt tgattggcca caacaggcga cgcgctttat cgaggcttac gttatccggc
    31801 caggagttca gcggtgcaaa acgttgctga ccacgacccg cgccgccgcg tccatcgatt
    31861 gaacggtaag tacctgcgcc gtgccaggcc atacgaataa acagaccggc gtagctgccc
    31921 cagtcggctg gccaccacgg ctgagattct gtcagcaggg ctttcagatc ttttttcagg
    31981 ccgtagtaat ctaatttgct gaattctttg cggtagtcaa agtcctcacc cagcgggtta
    32041 gaacgattag aatgttggtt taacaggtca acacggagtt gatttggcca ccagtcgcga
    32101 gtggttgtgc ccgcccccgc gctctggtcg tgaccgccct gatggaacgg gcatttgcca
    32161 gtggctgtcg tgttatggat atcgtctgac gtgctcatca atgtgctccc ctctacagtg
    32221 ttaccgttac gatacacagc gttagagaga agttataatt gaattaatcc acggatgcga
    32281 tagttgagat cttacatatt gttggttaaa gagatgtagg tcaaattgat cgtaattaag
    32341 cttttttgat tatgtccgaa ttcggacaaa tgctttataa aaaagggttt tagcttcata
    32401 tccttcaagt aatgagggag aaaacaaggc tgacaacctt attttcccgc cctcatttcg
    32461 aggcagcatt ttgtgctctg tttaaaattt gtgatcactg tgtgattttc acaaaagcca
    32521 cactatttat aaaccaggtc gaacacccag cgtatggcaa atcgcgtaac tcatttcagc
    32581 gcggttaagc gtatagaagt ggaaatcttt cactccttca cggcttaaaa tcttcaccat
    32641 atccatggca atattcgcgc ccaccagttt gcgggtttcg gcatcatcat ccaggccgtc
    32701 gaacatttgc gccatccacg ccggaatacg cacgttggtc atatcggcaa atttcttcgc
    32761 ctgtttaaag ttagataccg gcaaaattcc cggaataatt tccacatcaa tgcccgccga
    32821 tacacagcgg tcacgaaaac gcaggtagct ttcgacatcg aagaagaact gagtaatcgc
    32881 gcggttggct ccggcatcga ctttgcgttt cagattaagc aaatccgcct gagcgctttt
    32941 tgcttccggg tgaacttccg gatacgccgc cacggagata tcgaaatctg ccacttcttt
    33001 taatagcgtc accaggtcag aagcatacat ttctggctta ccacttcccg gcggcagatc
    33061 gccacgcagc gccacgatat gacgaatacc gttattccag tagtcgcgtg caatggtgcg
    33121 caactcgtcg ggcgtcgcat caatgcaagt aagatgcggt gccgcttcca gaccagtgcg
    33181 atctttaatg cctttaataa tgctgtgcgt acggtcgcgc tcgccggagt tcgcgccata
    33241 ggtcaccgat acaaacttcg gtttcaggct gctaaggcga tcgatggagt tccacagggt
    33301 ctgctccatt tcactggtac gcggcgggaa aaactcgaac gaaacgttaa tctgcccctg
    33361 gacttctgcc aggctctgat tcagggcatc ccgctggctg gcgtgaaaaa agctcatacc
    33421 ttacctcatc aatcgcttgt agttatatgt tgtgttgtgc acatctatcc gtttagacgt
    33481 ccagatgtaa agatgaagga aaagccgaag ggcgtcaacc gaaaaatgac caatgatggt
    33541 gaggaaaaat cagggaagat gaaaaaactt caggatggta agaaaaagaa aatccggtat
    33601 gcaaagcaaa ccggactgca aatataaact aaccatgtac ttattgcttc ttgccggatt
    33661 cgtcgtaaac gcttaatccg gcctaatcct atgataattc aatatatttc attagaaacc
    33721 ataaacccga aaacatgagt accgggcatt attaaatttc tgaaattaca acaactgtgc
    33781 cagccggttg atatccgact gaatcgcccc ggcggtgacg tcgcgccccg cgccaggccc
    33841 acggatcacc agcgggttgt cgcgatacca gcggctttcg atggcaaaga cgttatcgca
    33901 cggcagcagt gatgccagcg gatggtcttc acgcaccgcc tccacgccca cacgcgcttt
    33961 gccgttggca tcgaaacgcg ccacgtagcg cagcaccagc cccatttcgc gggccgcttc
    34021 cagccgttgc accatctgct cgttaagttc atcgccattt tcaaagaaat ggtcgatgct
    34081 gccgccttcg caatgagcag gcaccagcga ttccacacgt acctggtccg gttcaatgtt
    34141 gtaacccgct tcacgcgcca gaattaccag cttgcgcatt acgtctttgc cggagaggtc
    34201 gtcacgcgga tcaggctcgg ttaagccctg ctgccacgcc tgatccacca gctcggtaaa
    34261 cggcacgcta ccgtcgaatt gcaggaacag ccaggagagc gtgccggaga agatcccgct
    34321 gatcgacaaa atagtatcgc cgctgtcgat cagatcgcgc acggtgtggt tgatcggcaa
    34381 gcccgcaccg acggtggcat tgtacagcca gtgacgcccg gttttttcga agccgtcgtg
    34441 gatctggcga tatttattgc tgtcgctggc tcccgccagt ttgttggcgc tgataacgtg
    34501 gaaaccgtgg ctggcgaaat caagatactg atcagcaagc tgctggctgg cggtaacgtc
    34561 cagcaccact aaatcatcat acggatgggc gcgcatccac aggaacaacg actcttcatc
    34621 ctgctcaacc gcttcatcgt tgaagaaggc taacgcgcgg ctggcgtcca gcccgtcata
    34681 gctcaacagg ctgcggcggc tgtccaccac acctgccagc acaaactcaa agccggtacg
    34741 tgccgaaagc gtgctctgct cacgggcgaa cagttccagc caacgggaac cgatattgcc
    34801 cttaccgaac aataccaggc cgatgcgttt ttctgcgcgg aagacggact gatgcagccc
    34861 ctgaatcagg ctttcggtcg ggccggtgcg cagtactgcc accaggctga tgccgtcatc
    34921 ggactgccag gtaaattcga ccggctggcc tttcagttgc tgccagaagc ggtggcaatg
    34981 cagcgggtta cgggtgacgc ctgcaccgac catcgccacc agcgccagcc cctgacgcag
    35041 gcgcagttcg ccaggtaatc ccgcttcgtc gaggattttc agcgcactgt cggccacttc
    35101 tgaggtgtag caaaattgca gcaactggcg atcgttatgt acgccaaccg ccagcgggcg
    35161 tacctgcgcg cgtttcagga tttggtcgat ctctttatgc gccagtttga aatcctgact
    35221 ggcgggcacc tgaaactcaa tcaaacagac atcatcgtgg ctggtgacaa tacgcgcacc
    35281 agtaccggag gccagcacgc gttcaatgcg cgtggaacct tgatccggcg tgtagctaca
    35341 gcgcagttgc aggtcgattt cgctgccaga aaccggctgt aaagtacggg cgtgaagaac
    35401 gggagccgcc aggcgcgcca gttcgctggc ctcatccaga cgcagcaacg gcagcaggca
    35461 ggcatctttc actttacgcg ggtcggcact gtataccccg gcgacgtcgc tccagatggt
    35521 tacgcgagaa acacccgcca gcgcaccgat ttgtgtcgcg gaatagtcgg aaccgttacg
    35581 ccccagcagc accgtttcac cggcgttgtt gcggctgata aatccggtca ccaccagacg
    35641 tttgcccgga tgttgcacca gcagctgttg cagcaacggg taagaaagcc cttcatcaac
    35701 ctgcggttgt gcggcgcgtt cagcgcgtaa aaactcgcgg gcatcaagcc aggccgctgg
    35761 cagcccttgt tgattaagta ccgcagacat caggcgtgcc gaccatactt ccccgtggcc
    35821 caccacttcc gcatacactg cgtcgttaat accgctgtcg agcagcgccg ccaggcgctc
    35881 aaggtcgctg acaaaagcgc gaatgaggct atcagcttct tcagcgggta gcagaccgct
    35941 aatcagatcg cactgatagc gacgcagcgt ttgttgaacc tgatgcgcag agagacgatc
    36001 ggtctggctt agtttcaacc agttaatcaa ctggttagtg gtgctaccgg cggcggaaac
    36061 caccatcata tcgtcaggct gagagtactc cgccataatg cccgcaacac gcaaataaca
    36121 cttcacatca gccagactac tgccaccaaa tttatgcagt tgacgacctt tcgcccctgc
    36181 ctgcgcaatc acactcattt ttaccccttg tttgcagccc ggaagccatt ttccaggtcg
    36241 gcaattaaat cttcgccatc ttcaataccg gtggagatac gcagcagcgt ctcggagatc
    36301 ccggcggcag cacgcgcttc tggtgccatg cctgcatgtg tcatggttgc ggcgtgagag
    36361 attaaacttt ccactccccc taatgattcc gccagcgtaa acaacgacag cccgcccagg
    36421 aaacgacgca gcgtctgctc atcgccatcc agttcaaaac tcaacattgc gccaaagcct
    36481 ttttgctggc gcgcggcaat ttcatgcccc tgattttccg acagcgacgg gtgatacagt
    36541 tttttcacca acggctgggt ttgcagatat ttcacaatcg cctgcgcgtt gcgctgggcc
    36601 agctccatac gcggcaccag cgttcgcaac ccgcgtagca gcagatagct gtcaaacgcg
    36661 ccgcccgtca cgccaatatt gtttgtccac caggcaagtt cagtgacaac gtccgggtct
    36721 ttagcaatca ccacgccagc caccacgtca gagtgaccgt tcagatattt cgtacatgaa
    36781 tgcaacacca gatcggcacc caatgccagc ggattttgta atgccgggct taagaaggtg
    36841 ttatccacca cgctcaccgc cccgacttcc cttgccagat ggcagatttt cgcaatatcc
    36901 acgatgcgta acaatggatt acttgggctt tctaccagta ccagtttggg tttttctgcc
    36961 agcgctgccc gtaatgcctg ttcatcgcct tgatcaacaa acaacacgcg atagcaaccg
    37021 cgtttcgcca gactgtcgaa caggcgatag ctaccgccgt agcagtcgtg cggcgcaacc
    37081 agcagatcgc caggtttcaa aaagacggtc gttaccaagt gaatcgcgga catgccggta
    37141 ttagtaagta ctgcgccagc accgccttcc agttctgcca gcgcacgctg aaccacatcg
    37201 cgcgttgggt tgccgcgacg cgagtaatca tgcgcgcgcg gttcattaaa tccggtaaag
    37261 ttataggtgc tggaaagatg gatgggaggg acaacgcaac catactgttc gtcgtcattt
    37321 aacccgctac gcactgcgat ggtggcctgt ttacgcgtca tgtgatgaag tttcctgggc
    37381 tttgtcggtg aaatgtcagg caccagagta aacattgtgt taatggacgt caatacatct
    37441 ggacatctaa acttctttac gtatagattg agcaaatccc aaatagccgt taaaattata
    37501 tgcattatca cgccgacagg tgcattacac gatgtcacgg taacgcctgt acggtaaact
    37561 atgcgggttt acggtcagta cccacataaa ttgtgtggtc tggtctcaat ttattgacga
    37621 agaggattaa gtatctcatg gctgaatgga gcggcgaata tatcagccca tacgctgagc
    37681 acggcaagaa gagtgaacaa gtcaaaaaga ttacggtttc cattcctctt aaggtgttaa
    37741 aaatcctcac cgatgaacgc acgcgtcgtc aggtgaacaa cctgcgtcac gctaccaaca
    37801 gcgagctgct gtgcgaagcg tttctgcatg cctttaccgg gcaacctttg ccggatgatg
    37861 ccgatctgcg taaagagcgc agcgacgaaa tcccggaagc ggcaaaagag atcatgcgtg
    37921 agatggggat taacccggag acgtgggaat actaaagcaa aaaagagcgg cgcggagtgg
    37981 aatcgcctga tgcgctacgc ttatcaggcc tacgtcatat tgcaatttat tgaatttgca
    38041 cgaacttgta ggccggataa ggcgttcacg ccgcatccgg cataaacaac gagcacgttg
    38101 tctgcgaccc accgcttttt atacatggac gtttaactat gaaaaacagg ctgctgatcc
    38161 tcagcctgct ggtttctgta cctgcctttg cctggcagcc acaaaccggc gacatcatct
    38221 ttcagatctc tcgctcatcg caaagtaaag cgatccaact ggcgacccat tccgattata
    38281 gccacaccgg tatgctggtg atacgcaaca aaaagcccta cgtttttgaa gcggtcggcc
    38341 cggtgaaata caccccgctc aagcagtgga tcgcccatgg tgaaaagggc aaatacgttg
    38401 ttcgccgcgt tgaaggcgga ctgagtgttg aacaacagca aaaactggcg caaacggtaa
    38461 aacgttatct cggtaaaccg tatgatttca gcttctcatg gagcgacgat cgccagtact
    38521 gttcggaagt ggtgtggaag gtttaccaga acgcgctggg aatgcgcgtg ggcgagcagc
    38581 agaagttgaa agagttcgat ctcagcaacc cgctggtcca ggctaagctc aaagaacgct
    38641 acggcaaaaa tattccgctt gaggaaacgg tagtttcacc acaagccgtt ttcgacgcgc
    38701 cacaactcac cacggttgcc aaagagtggc cgctgttttc gtggtaatgg atgatgaaaa
    38761 aaataattcc cgccgtagtc tgtttgttgc tttgtagttg cacgaccttt ccgcaaactg
    38821 tcgctccggt aaacggtgat gcctccaatt agttgaatta aggatataat actcgctttt
    38881 aaggttgttt catggtcaca gttaaccttc attgcccccg ttgccagtcc gttcaggttt
    38941 accgtcacgg gcagaaccca aaaagacatg atcgattccg cggccgtgac tgtcaccggg
    39001 tatttcagct cagttactgt tacgagcccc gtaaaccggg agttaaagac caaatcgctg
    39061 aaatgacttt caacggtgcc ggagttcgcg atactgcccg tacgctgaaa atcggcatta
    39121 atacagtcat ccgaacttta aaaaactctc gccacgaaga ataacgtcat ccccggtcgc
    39181 tcatgctgat gtggcactca tctgcgaact tagtcagcaa tggagctttg tcggcagtaa
    39241 agcgcgacag cactggctct ggtacgtgta caatacgaaa actggtggtg ttctggccta
    39301 cacttttggc ccccggactg acgaaacctg tcgtgaattg ctggcgctgc ttaccctttt
    39361 accatcggca tgttgaccac tgacgaatgg ggtagttaca ccagagagtt accgaaggaa
    39421 aagcatctga ctggtaagat ttttactcag cgcattgagc gcaacaatct gaccctgaga
    39481 acccgaatca aacgtctggc ccgcagaacc atctgtttct cgcgctctgt agaacttcat
    39541 gaaaaagtca tcggtgcttt tatcgaaaat atatgttcta ctaattggag tcatcaccct
    39601 tctgatgagc tttccttgga tgattaccag actgaataca cagccaaaga tttttacctc
    39661 attctgctct tttttctcaa atgacatata ttatatttta catcatattc ataggcatat
    39721 gacattcccc cgcccccatt atgtcattgt gggttgatgt tgggctttca taaaaaaaac
    39781 atcttttttt gaactagcat taattatatt tactttatat attaaaatat tttctccatc
    39841 tggaaatatt ttagccacac aatataaaac agctgaatcc gaattactta aaaatatatt
    39901 atcattaccc ataactccag ccattaaatt actggagact aaagcaagaa aataacaaca
    39961 ttgtattgcc ttcattttaa ttactccctt gtctgataat tgtaactcca ttccctttag
    40021 ctttgggatt atattttaga taggtg
//
LOCUS       DAJTMK010000014        39779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTMK010000014 DAJTMK010000000
VERSION     DAJTMK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39779
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.014"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            6..437
                     /locus_tag="OO135_000629"
     CDS             6..437
                     /locus_tag="OO135_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5396583.1"
                     /translation="MALNLASGEGYFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFS
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            520..1422
                     /locus_tag="OO135_000630"
     CDS             520..1422
                     /locus_tag="OO135_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR5396584.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1467..3629)
                     /gene="uvrD"
                     /locus_tag="OO135_000631"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1467..3629)
                     /gene="uvrD"
                     /locus_tag="OO135_000631"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5396585.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVDTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3713..4429)
                     /gene="yigB"
                     /locus_tag="OO135_000632"
     CDS             complement(3713..4429)
                     /gene="yigB"
                     /locus_tag="OO135_000632"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5396586.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLSKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4429..5325)
                     /gene="xerC"
                     /locus_tag="OO135_000633"
     CDS             complement(4429..5325)
                     /gene="xerC"
                     /locus_tag="OO135_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5396587.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRSFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5322..6029)
                     /locus_tag="OO135_000634"
     CDS             complement(5322..6029)
                     /locus_tag="OO135_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5396588.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6026..6850)
                     /gene="dapF"
                     /locus_tag="OO135_000635"
     CDS             complement(6026..6850)
                     /gene="dapF"
                     /locus_tag="OO135_000635"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5396589.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6887..7090)
                     /locus_tag="OO135_000636"
     CDS             complement(6887..7090)
                     /locus_tag="OO135_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5396590.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            7553..7873
                     /gene="cyaY"
                     /locus_tag="OO135_000637"
     CDS             7553..7873
                     /gene="cyaY"
                     /locus_tag="OO135_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5396591.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7913..10459)
                     /gene="cyaA"
                     /locus_tag="OO135_000638"
     CDS             complement(7913..10459)
                     /gene="cyaA"
                     /locus_tag="OO135_000638"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5396592.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHMAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10846..11787
                     /gene="hemC"
                     /locus_tag="OO135_000639"
     CDS             10846..11787
                     /gene="hemC"
                     /locus_tag="OO135_000639"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5396593.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLGDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11784..12524
                     /gene="hemD"
                     /locus_tag="OO135_000640"
     CDS             11784..12524
                     /gene="hemD"
                     /locus_tag="OO135_000640"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5396594.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRTLILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12546..13787
                     /gene="hemX"
                     /locus_tag="OO135_000641"
     CDS             12546..13787
                     /gene="hemX"
                     /locus_tag="OO135_000641"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5396595.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            13790..14986
                     /gene="hemY"
                     /locus_tag="OO135_000642"
     CDS             13790..14986
                     /gene="hemY"
                     /locus_tag="OO135_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5396596.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15666..15827
                     /locus_tag="OO135_000643"
     CDS             15666..15827
                     /locus_tag="OO135_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396597.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15911..17146)
                     /locus_tag="OO135_000644"
     CDS             complement(15911..17146)
                     /locus_tag="OO135_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5396598.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDMLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17293..17369)
                     /locus_tag="OO135_000645"
     tRNA            complement(17293..17369)
                     /locus_tag="OO135_000645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17333..17335),aa:Pro,seq:tgg)
     gene            complement(17412..17498)
                     /locus_tag="OO135_000646"
     tRNA            complement(17412..17498)
                     /locus_tag="OO135_000646"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17462..17464),aa:Leu,seq:cag)
     gene            complement(17519..17594)
                     /locus_tag="OO135_000647"
     tRNA            complement(17519..17594)
                     /locus_tag="OO135_000647"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17559..17561),aa:His,seq:gtg)
     gene            complement(17653..17729)
                     /locus_tag="OO135_000648"
     tRNA            complement(17653..17729)
                     /locus_tag="OO135_000648"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17693..17695),aa:Arg,seq:ccg)
     gene            complement(17832..19217)
                     /locus_tag="OO135_000649"
     CDS             complement(17832..19217)
                     /locus_tag="OO135_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5396599.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19408..20148)
                     /gene="rffM"
                     /locus_tag="OO135_000650"
     CDS             complement(19408..20148)
                     /gene="rffM"
                     /locus_tag="OO135_000650"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5396600.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20151..21503)
                     /gene="wzyE"
                     /locus_tag="OO135_000651"
     CDS             complement(20151..21503)
                     /gene="wzyE"
                     /locus_tag="OO135_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5396601.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21500..22579)
                     /gene="rffT"
                     /locus_tag="OO135_000652"
                     /gene_synonym="wecF"
     CDS             complement(21500..22579)
                     /gene="rffT"
                     /locus_tag="OO135_000652"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5396602.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPG
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22576..23826)
                     /gene="wzxE"
                     /locus_tag="OO135_000653"
     CDS             complement(22576..23826)
                     /gene="wzxE"
                     /locus_tag="OO135_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5396603.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23828..24958)
                     /gene="rffA"
                     /locus_tag="OO135_000654"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23828..24958)
                     /gene="rffA"
                     /locus_tag="OO135_000654"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5396604.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHIFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24963..25637)
                     /gene="rffC"
                     /locus_tag="OO135_000655"
                     /gene_synonym="wecD"
     CDS             complement(24963..25637)
                     /gene="rffC"
                     /locus_tag="OO135_000655"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5396605.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25615..26496)
                     /gene="rfbA"
                     /locus_tag="OO135_000656"
     CDS             complement(25615..26496)
                     /gene="rfbA"
                     /locus_tag="OO135_000656"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5396606.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26515..27582)
                     /gene="rffG"
                     /locus_tag="OO135_000657"
     CDS             complement(26515..27582)
                     /gene="rffG"
                     /locus_tag="OO135_000657"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5396607.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27582..28844)
                     /gene="wecC"
                     /locus_tag="OO135_000658"
     CDS             complement(27582..28844)
                     /gene="wecC"
                     /locus_tag="OO135_000658"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5396608.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28841..29971)
                     /gene="wecB"
                     /locus_tag="OO135_000659"
     CDS             complement(28841..29971)
                     /gene="wecB"
                     /locus_tag="OO135_000659"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5396609.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30027..31073)
                     /gene="wzzE"
                     /locus_tag="OO135_000660"
     CDS             complement(30027..31073)
                     /gene="wzzE"
                     /locus_tag="OO135_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5396610.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31085..32188)
                     /gene="wecA"
                     /locus_tag="OO135_000661"
     CDS             complement(31085..32188)
                     /gene="wecA"
                     /locus_tag="OO135_000661"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5396611.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32428..33687)
                     /gene="rho"
                     /locus_tag="OO135_000662"
     CDS             complement(32428..33687)
                     /gene="rho"
                     /locus_tag="OO135_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5396612.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33772..33873)
                     /gene="rhoL"
                     /locus_tag="OO135_000663"
     CDS             complement(33772..33873)
                     /gene="rhoL"
                     /locus_tag="OO135_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5396613.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34014..34343)
                     /gene="trxA"
                     /locus_tag="OO135_000664"
     CDS             complement(34014..34343)
                     /gene="trxA"
                     /locus_tag="OO135_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5396614.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34474..35739
                     /gene="rhlB"
                     /locus_tag="OO135_000665"
     CDS             34474..35739
                     /gene="rhlB"
                     /locus_tag="OO135_000665"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5396615.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35873..37357
                     /gene="gppA"
                     /locus_tag="OO135_000666"
     CDS             35873..37357
                     /gene="gppA"
                     /locus_tag="OO135_000666"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5396616.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVDQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37404..39425)
                     /gene="rep"
                     /locus_tag="OO135_000667"
     CDS             complement(37404..39425)
                     /gene="rep"
                     /locus_tag="OO135_000667"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5396617.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9869 a        10813 c        10058 g         9039 t
ORIGIN      
        1 agaaggtggc gttaaatctc gcttcaggtg aagggtattt ctttatccgt cctggcggcg
       61 tgttttatgt cgcgggagat aaagtcggca tcattcgtct ggatgccttt aaaaccagta
      121 aagagattca gttttcggtg cagtcagggc caatgttatt agaaaacggc gtaattaatc
      181 cacgcattca cccgaatgtt gcctcgcgta aaattcgtaa cggtgtaggg attaataaac
      241 aagggaacgc cgtgtttttg ttgagccagc aggcgacaaa tttttatgat tttgcctgtt
      301 atgccaaagc gaaactgaac gttgagcagt tgctttatct tgatggcaca atttcacata
      361 tgtatatgaa aggcggggca atcccgtggc aacgctatcc ttttgtcacc atgatttccg
      421 tcgagcggaa aggttaagca ttgtacactc gaggaggcca gacaaactgg gactggctac
      481 acagctggaa aatcatcaga taaatgaagg gaaacaaaaa tgaaaaaaac gctcgcaacg
      541 ttatttttat taacgtgtct gggatcatca tccgcctatg cggataacgc tttgatttta
      601 caaactgatt ttagcctgaa agatggtgcc gtctcggcga tgaaaggggt cgcattcggc
      661 gttgaccata atcttaaaat ctttgattta acacacgaaa ttccaccgta taacatctgg
      721 gaaggcgctt atcgcctgta tcagactgcc agttactggc cgcaaggttc ggtgtttgtt
      781 agcgtggtcg atccgggcgt aggcactgac cgtaaatcgg tagtgctgaa aaccaaaaac
      841 ggtcaatact tcgtttcacc agataacggt acgctcactt tggtggcaca aactctgggt
      901 attgatagcg tccgggaaat cgacgaaaaa gctaaccgcc tgaaaggttc tgaaaagtca
      961 tataccttcc acggtcgtga cgtgtatgcc tacaccggtg cacgtctggc ttccggggcg
     1021 attacgttcg aacaggtggg gccagagctt ccagcaaaag ttgtcgaact ttcttatcag
     1081 aaagcaaaag caacaaaagg tgaagtgaag ggcaatatcc cgattctcga tattcagtac
     1141 ggcaacgtct ggagcaatat cagcgacgag ttgctgaatc aggcagaaat caaactcaac
     1201 gacacgttgt gtgtgaccat ttccgaaggt tcccagcaaa aatacgcagg gaaaatgccg
     1261 tatgtcgcaa gcttcggcga tgtgccggaa ggccagccga tggtgtatct gaacagctta
     1321 ttgaatgttt ccgtggcgct gaatatggat aatttcgcgc agaaacatca ggttgcgtct
     1381 ggtgctgact ggaatattga tgtgaagaag tgcgctaagt aaggtgctga tgcggaatgc
     1441 ggtgtggcgc accgcatccc gcaacgtcac accgtctcca accgggcata agccgccacc
     1501 agccatttaa taccctggcc ctggaatgcc acctgcaaac ggctatgttc accgctgcct
     1561 tccatattga cgatggtgcc ttcaccaaac ttagcgtggc gtacacgctg gccgagcttg
     1621 tagccgctgt cgttctcgac catcggcgta cccatccgct gatggctgac cgggcggctt
     1681 accgtggcgc gcaggcgcac ctcttccaca cactcttccg gcagctcgcc aataaagcgc
     1741 gacgggcgat ggtaaacctc tttgccatac agacggcgag tttccgcgta ggtcagcgtc
     1801 agtttctgca tcgcgcgggt tacgccaacg taggccagac ggcgttcttc ttccagacgc
     1861 ccgccttcat ccagcgacat ctggcttggg aacatgccct cttccatacc gacgataaac
     1921 acctgcggga actccagacc tttcgccgag tgcatggtca tcagttgtac cgcatcctgc
     1981 caggtatccg cctgcccttc gcctgcttcc agcgccgcat gggataggaa cgcctgcagc
     2041 ggcattaaat cttcgtcttc ttcgttgtag ctgaactggc gcgttgccgt caccagttcc
     2101 tctaagtttt cgatacgcgt ctgacctttt tcgcccttct cctgctcgta catggtacgc
     2161 aggccggagt ctttaattac ccggtcagtc tgtacatgca gcggcatatc ggcagtttcc
     2221 tgcgctaagg cgtcgatcag ttccataaac cgctgtaagg cgctggcagc acgtccggcg
     2281 agggcttttt cctgcaacag ttcacgacat gcctgccaga gtgttaactg gcgatcgcgc
     2341 gatgtctgac gtaccacgtc cagcgtccgg tcaccaatac cccgcgttgg cgtattcacc
     2401 acacgctcaa aggccgcgtc gtcgttacgg ttggcaatca ggcgcagata cgagagcgca
     2461 tctttgattt cctggcgttc gaagaagcgc atcccgccgt aaatacggta cggcatactc
     2521 gcctgcaaca gtgcttcttc cagcacacgc gactgggcgt tactgcggta gagaatggcg
     2581 cactcggcaa gcgcccctcc gttgtcctgc caggttttga tgcggttaac cacaaaacgc
     2641 gcttcatcga gttcgttaaa agcgcaatag agggaaatag gctcaccgtc cgcgccatcg
     2701 gtccacagtt ttttacccag acgcccgtta ttgttttcaa tcagggcgtt agcggcgctc
     2761 agaatattgc tggtagagcg gtagttttgc tccagacgaa tagtttcggc accggggaaa
     2821 tcattaagga aacgctgaat attctccacc tgcgccccgc gccagccgta gattgactgg
     2881 tcgtcatcac cgacgatcat cactttgccg gtgtcgcccg ccagcaggcg gatccacgcg
     2941 tactgaatgt tgttggtatc ctggaattcg tccaccagga tattggtaaa acgttcgcgg
     3001 tagtgttgca ggatatgcgg cttgttaagc cacaactcgt gagcgcgcag cagcagctcg
     3061 gcgaagtcca ccaggcccgc gcggtcacac gcttcctgat acgcctgata caccttctgc
     3121 caggtctgct ccaccggatt accgtagctt tgaatatgat gtggacgcag gccttcatct
     3181 ttctggctgt tgatgtacca cattgcctgg cgcggcggcc actgcttctc gtcgaggttc
     3241 atcgccttga ttaggcgttt gagcaggcgt agctggtcct cgctgtcgag gatctgaaaa
     3301 tcctgcggca gattggcgtc catatggtgc gcacgcagca gacggtgcgc cagcccgtgg
     3361 aaggtgtcga cccacatgcc gccctggctg gtgcccatta gctgcccgat acgatgacgc
     3421 atctccgccg ccgctttgtt ggtaaacgtc accgccataa tcgagtatgg cgagcagttt
     3481 tccacgctca tcagccaggc gatacgatgc accagtacgc gcgtcttacc actgcccgcg
     3541 cccgccagca ccagaaggtt gctgcgtggc gcggccaccg cttcgcgctg tttgtcatta
     3601 aggctgtcga gcaggtaaga aacgtccatt ggcaccgccg cgtaaaaata ggttgggcgg
     3661 agaagcgcaa cgccctccgc caaaaagctg ggtatatata cagatttgct gattatatca
     3721 gcgaggtcag agatgccaac cgggaaattt ccagatgcgg cagtaaacgg ctgtcccagg
     3781 tttgcatcag atcgccattt tccggtctga tccaacaggc ctgcattccg ctgcgaattg
     3841 ccccacccac gtcagtggtg agatcgtccc caacatgtaa gatctcgccg atcggcacgt
     3901 tgagtttttc cgcagccaga aagtacatat cgctgaacgg ttttgagcgc ccgtgcgggc
     3961 cagcgcgcag cacaaactca aaataatccc ccaaaccaaa cagttccggc tgggcgttac
     4021 cgttggtgat cgccaccagc ggccatttct tcgacagctg ttttaaggtg tcgtgagttt
     4081 gctgcgggac gtcgattcgg ctgcgccatt tggcaaagtt gatcattgct gcgtgtgcgc
     4141 ctgcactggc ttcttcggca ctcagcccgg cgtcgagcat cgcttgttca atcgaacgaa
     4201 aacgccagcg cgtcacgtcg tgataaatct cgggttccgc ttcccgtacc gcctggcgca
     4261 ggcgttgcag atcttcattc tggaagctgc gcagcgccgg atgataattt tgcacaaagg
     4321 taagcgcctc tcgctcggtg cgcaaaatca ccggacggtt atcgtaaagg gtatcatcca
     4381 ggtcaaaggt gagcgccgag atgcgcccca aaggccggta aaaacgcatt atttcccccg
     4441 tttggcgcgt ggatgcgccg catcgtacac cgaggcaagg tgttgaaaat caagatgagt
     4501 atagatttgc gtggtggaga ggttggcatg acccagtagc tcctgcacac cacgaagatc
     4561 gccgctcgac tccagcatat gcgtagcgaa cgagtgacgt aatttatgtg ggtgaacgtg
     4621 attattcagc ccttgtttta tgccccattc ggcaaagcgt ttctgcacat tacgcgcgga
     4681 gatacgcttg cccagtttcg acagaaaaag cgcgtcgtct tcgctaccaa acaggtcgcg
     4741 caaatcaagc cagtgctcaa tccacgccac agcgttgcga ccaatcggca ggcggcgctc
     4801 tttgctgcct ttccccatta cccacacttc accagactcc aggtcgaggt gtttgatatc
     4861 cagccccacc agctcggaaa gacgcagacc agcgccgtac atcacttcca gcattgcacg
     4921 gtcgcgtaca gcgaggggat cgttgatatc aatatccagc agccgattca tatcgtcgac
     4981 gtcgatattt ttcggcagat gacgcggcgc tttcggtgcc gaaacacctt tcgccgggtt
     5041 agctttgagt tcgttctggc tgaccagcca gtcaaaaaag ctacgtagcg cagaaagccg
     5101 taacgccaga cttgctgctc ccagcccttt acggcgactg cgtacagcaa aactgcgcac
     5161 catcgtcaca tcacattgct gccagctttg caggccgttt tcgctggcaa aattgatgat
     5221 cgcctcaagc tgacgctggt agttaagcag ggttatcggg ctaagctggc gctccacgct
     5281 cagataacgt aggtagcgtt ctacatcggt gtgtaaatcg gtcatacgcg ttcaatccaa
     5341 cgctccagaa gctccggcaa catcagcgca atttcatgaa gtaactgcgt tccttgccct
     5401 tgttgatagt gactggcatc gcgactggta aacagcacga cacccaaatc agcatcgctt
     5461 cccagcatcg acatcgccac cgatcccacc gctttcgctt ccggtagcac caccagcagc
     5521 tctggtccgt taagcggccc aagatagtgc tgttcctgcc ccaaacgctg aatacgcagc
     5581 ggttcgaaag actgacggct taatgccaga tgagtgtggt tcgacggcgc acctaagcgc
     5641 cagcgatccg gaaacaggcg cagactcgca cctgccaggc cgagatcgcg cgcccagcgg
     5701 tgaaagcgca tcagcatatc gtcgagactg ctggcggcgg tgagactgcg ctgcaggtag
     5761 agtaggcgat aaaacaggcc ttcgttggcg atagcctgtt ccatcaacag cgccatgttc
     5821 tcttccagaa catgaatatg attacgtgcg cgggccatgt gccactcgac caacgaaacg
     5881 gtgccgcgta ccggatgcgg cacacgtatc gcttctactg cgcgcgcatt acggataaaa
     5941 aactcaggat ttttaatcag ataatcgaca accgcccggt catcaagctc cgtgagtgtt
     6001 tcctgcagtt cttcccctgg ttgcttcata gatgaataaa tccgtcgtag acatgtaccg
     6061 ccgggccagt catatataac gggtgacccg gacctttcca ggcgatatca agacgaccgc
     6121 cggggagttc cacgcgtact tcttcggcca gcaaaccttg ctgaatccct actgcaaccg
     6181 ccgcacacgc gccgctgccg caggcctggg tttctcctgc cccacgctca taaacgcgta
     6241 aacgaatatg ctcgcgctta accacttgca taaaaccgat attggcgcgc tccggaaaac
     6301 gctcgtggct ttccagaaca ggaccaagcg tttctaccgc cgcggtatcg acatcatcga
     6361 cctgaatcac gcaatgcgga tttcccatcg acaccacgcc gcataagatt gtctgctcgg
     6421 cggcgcgcat aatataggtc ttttccgctt tgttagcgcg aaacggcaca gcggaaggtt
     6481 cgaagttggg ttcgcccata tttacgcgga ccagatcatc atcggtgacg gtcagaacca
     6541 tccgcccgtt ggcggtgctg acgcggatat cgcgcttatt ggtcagccct ttcaggcgca
     6601 caaaacgggc aaagcagcgc gcaccgttgc cgcactgcgc cacttcactg ccatcagcat
     6661 tgaaaatgcg atagtgaaaa tccagttcag gatcatacgg cggctcaacc accagcagct
     6721 ggtcaaaccc tacccccagg tgccgatccg ccagccgacg aatcagctcc ggtgaaaaaa
     6781 agacattctg cgttaccgcg tcgacgacca taaaatcgtt gccaaggcca tgcattttcg
     6841 agaactgcat catttactcc aatcacgcgg gtacagaaac tgacttttag taattcacct
     6901 gggatggacc atcgccagtg gcgcgatcgt ttttatccgg caccgtggat tgcgtttgcg
     6961 tctctaccgg tttggtcggc ggcggtgcat ttttatctgc aggcgggaaa tagagcggac
     7021 ctttcagacc gcagcccgtc aggctgaaga gagtaagtaa tacagtgagt accttaaaca
     7081 cgtttttcat tatggattgc ctgtaaagtt catcgctttc tgctttctat catcgcagga
     7141 gaaggcgtaa aagcaagcgt ttagcgactc tttatgaagt ctgaaaagtg aggaaatgct
     7201 gcgagtataa cgcttactcg tgaggattta tgttctgctg gcagtttcgt gatcggcacc
     7261 tcgcgccagg atggctttat gcactatcgc aatgtggttt gtttgttggc ttttttagtt
     7321 ccgtcagcgt gaggatgctc gctcggtaaa cctgatcata atgtttacca gaaacaggga
     7381 aaggcgtggt gtaactgcgc cctaatgaag agacaaatct ttcgccacac agagacatca
     7441 ttattccggt gacggatgaa tcatgccgga acgcagcagc agaggtgcaa actttgaagc
     7501 cctgacccca tgccgctata ctgccgccat cacttaaaaa caggatacaa caatgaacga
     7561 cagtgaattt catcgcctgg ctgatcaact gtggctgacc attgaagagc atctggacga
     7621 ctgggatggc gacagcgata tcgactgcga aatcaacggc ggcgtgctga ccattacctt
     7681 tgaaaacggc agcaaaatca ttatcaaccg ccaggaaccg ctgcaccagg tatggctggc
     7741 aaccaaacaa ggcggctacc attttgatct gaaaggcgat gagtggattt gcgatcgcag
     7801 cggcgaaacc ttctgggatt tgctggaaca ggcggcgacg cagcaggcgg gtgaaacagt
     7861 cagtttccgc taagattgca tgccggataa gcctcgcttt ccggcacgtt catcacgaaa
     7921 aatattgctg taatagcggc gtatcgtgat cctgattggc aggcggcatg ttaccgatag
     7981 attttgtgcg gaacggaatc acctgttcac gaccatcaac cttcacaatc tgatagaact
     8041 gcggcaggtt gaagttgatg aagcttgagc cgtaggtgaa gcggtcgtgc gatgacgagt
     8101 agaagcgact gacgtcacgc accagttcct ctttgctgcc ttcgcagtgg tgatacacct
     8161 caacccggtt gctttcgtca agaatgtaga tattaaagcc attctcgtct tgcgtttctt
     8221 cgaagaagaa ctggatgatc ccttcactag caaagccgtc caccaccgcc ggtaatttga
     8281 cgtgattggt ttcaacctgc actgacaggc cgtgcagttt gttatgcgaa atagcgccat
     8341 aaaactcgat ggcgttctcc agcttctgta ccgatacatt caggcgttcg aagaacaagc
     8401 cccaggtttg accagaaacg cgcagcgcct tgaaacgccc ggtttcctgg cgggtgctgg
     8461 aaaggcgcag ttcaatacac tcagaaacca gttgctgcac gcgagtacgg attaacccgc
     8521 gcagatgctg gctataacag aagacttcca cgctatctgg cggtgcggcg tcctgatgca
     8581 ttttgccgag aatagttttc agggcttcga tcatcgattg ctcgccgttg aagtgcagcg
     8641 tacgcacttc gttccacgag ttgcggtaca gcaggtcaac gctacctacc aggcaatttt
     8701 gattctcgcc aaagctgaag acatccagct tacggaaatc aaaatgcacc acctgattgc
     8761 ggaacgccgc tgtcgggtca tattccaggt taacgataat cgccatatgg cggatctcac
     8821 acgggctgta gagcgccttc ggtgtcggtg caggtaagcg cagcgggaaa tggtgcgaca
     8881 cgtcggcgac catctcctgc aacttaggca aatcgacaat gccgttacct ttaatataca
     8941 aacgggtgcg cgaggtcagc aggccgttaa accatgccca cgccaccagt ttgttcaggt
     9001 aacggttata ttccagcggc tgatggctga tgatcgactc aatatttggc gcgcggttat
     9061 acagatacca acctgaacgg ttagcacggc ccggcggcac ataaataaag gtcagattcg
     9121 gttccgagag atcgggtgaa atctgcgggt ttaccagcgt cactttacct ggtaatgctt
     9181 caaacgcggc atacagctta cgcgtcagca cgccgatatc ctgcggactg gcggagacgc
     9241 taaggttatt gcgacgcgca aagcggatca gattacggta gctctgcatc atcgcgtcga
     9301 gcaactcgtt gtgcgcctca cgcacctgat caatcttcca gttagcgcgg ttatcgagca
     9361 ttgccagacg agcttcgtcc caaccccact cgctcactaa ctggctcaac actgcgcgac
     9421 gccagcctac gcaggcgcgt tcacggctga gcttttcgca cacttttaaa tagaagcagc
     9481 gacgtactaa atccagacgg gtaaaatctt caatcgccgt caggtattca gtaacacgct
     9541 ccagcatcat gcagtatgga tcgagaccaa acgatacaat ctcaccgtcg tgcaaacgct
     9601 gtttgatatc tttcgccagc agacgtgggt tcgggtattc ccaggaatag gcttccagca
     9661 gcagtgtttt cagtaccgct ttgtatgggg aatcgatact cttgtagagc tgccaaaggc
     9721 tggcaccaaa gtactcttca gcagaaagcg agcttaagcc accgagatcc agccattcat
     9781 ttggcgtcag cacgccctgc gcgtaaagcg tcatcacata gtcgtcgtaa tgctcttctt
     9841 cgtcgcacgg caccatattc cacagaatac gcttaccggc gagacgcacg gcggtacgat
     9901 aaaattcgtc aagcagcagt atatgctggg tggagccaca atcttcgccc cccaggctgc
     9961 cgctttcatt atgacggaag cggttttcat caatcaggaa gaagctgact tccacaccca
    10021 gcgaggcggc ccagctttcc agcagactac atttacgttg tagtaactgg cgctcttcgc
    10081 tatcgagcca ggattgatga cagacccaga tatccaggtc agaggaacaa ctttgcccta
    10141 cggacgaggt gctgcccatg gtgtatacac cagtaattgg aagctcacct ttcggtggat
    10201 cctgtactga cattccacga tacagttcaa gctcgttcag gtagtggcgt tgagtttcat
    10261 caggcgtgta aaggcaaatg cctttgggaa cgttaccatc aaggtaaccc ggcattagcg
    10321 gatggtgata gtgcaacaat gtcggcagta gactgtagac ctgttggaat gcaggcccca
    10381 tagcagcaag cgcgcgatcc acacgcaatt gatttatggc atccagtctc tgtttcagag
    10441 tctcaatata gaggtacaag acgtatcgcc tgatttgcta cccgtcatga ctgtgattcc
    10501 gccaacatca acggtaacac gcggcattcg ggatatttcg tatgtcaaag gtaaccgtta
    10561 ccacttttcg cgcctggttt ttttagtttc acgacgaaaa aatggtctaa aacgtgatca
    10621 atttaacacc ttgctgattg accgtaaaga aagatgcgct acatacaagt gtagcaccgt
    10681 ttattctctg taaattcctt attacgacgg cgtgaaacgc ctgtcaggat ccactgccag
    10741 acctcatttt acggtttgcg caggcgtcta cgtttcacca caacactgac atcactctgg
    10801 caaggatgtt aggatggacc acggatgata atgacggtaa caagcatgtt agacaatgtt
    10861 ttaagaattg ccacacgcca aagcccactt gcactctggc aggcacacta tgtcaaagac
    10921 aagttgatgg cgagccatcc gggcctggtc gttgaactgg taccgatggt gacgcgcggc
    10981 gatgtgattc ttgatacgcc gctggcgaaa gtaggcggaa aaggcttatt tgtaaaagag
    11041 ctggaagtcg cgctcctcga aaatcgcgcc gatatcgccg tacattcaat gaaagatgtg
    11101 ccggttgaat tcccgcaagg tctgggactg gtcactattt gtgagcgtga agatcctcgc
    11161 gatgcctttg tgtccaataa ctatgacagt ctggatgcgt taccagcagg cagtatcgtc
    11221 gggacgtcca gtttacgtcg ccagtgtcaa ctggctgaac gccgcccgga tctgattatc
    11281 cgctccctgc gcggcaacgt cggcactcgc ctgagcaaac tagataacgg cgaatacgat
    11341 gccatcattc ttgccgtagc cggactaaaa cgtttaggtc tggagtcccg cattcgcgcc
    11401 gcgttaccac ccgagatttc tcttccagcg gtaggacaag gtgcggtcgg tattgaatgc
    11461 cgccttggtg atgcacgcac tcgcgagctg cttgccgcgc tgaatcacca cgaaactgca
    11521 ctgcgcgtta ccgcagaacg cgccatgaat acccgtctcg aaggcggatg tcaggtgcca
    11581 attggtagct acgccgagct tattgatggt gaaatctggc tgcgcgcgct ggtcggcgcg
    11641 ccggacggtt cgcagattat tcgcggtgaa cgccgcggtg cgccgcaaga tgccgaacaa
    11701 atggggattt cgctggcaga agagctactg aataacggcg cgcgcgagat cctcgctgaa
    11761 gtctataacg gagacgcccc ggcatgagta tcctggtcac ccgcccgtct cccgctggag
    11821 aagagttagt gagccgtctg cgcacactgg ggcaggtggc ctggcatttt ccgctgattg
    11881 agttttctcc gggtcgacaa ttaccgcaac ttgctgatca actggcggcg ctgggggaga
    11941 gcgatctgtt gtttgccctc tcgcaacacg cggttgcttt tgcccaatca cagctgcatc
    12001 agcaagatcg taaatggccc cgactacctg attatttcgc cattggacgc accaccgcac
    12061 tggcgctaca taccgtaagc ggacagaaga ttctctaccc acaggatcgg gaaatcagcg
    12121 aagtcttgct acaattacct gaattacaaa atattgcggg caaacgtacg ctgatattac
    12181 gtggcaatgg cggtcgtgag ctaattgggg ataccctgac agcgcgcggt gctgaggtca
    12241 ctttttgtga atgttatcaa cgatgcgcaa tccattacga tggtgcagaa gaagcgatgc
    12301 gctggcaatc ccgcgaggtg acgatggtcg ttgttaccag cggtgaaatg ttgcagcaac
    12361 tctggtcgct gatcccacaa tggtatcgtg agcactggtt actacgctgt cgactattgg
    12421 tcgtcagtga gcgtttggcg aaactcgccc gggaactggg ctggcaagac attaaggtcg
    12481 ccgataacgc tgacaacgat gcgcttttac gggcattaca ataactctca taacaggaag
    12541 ccataatgac ggaacaagaa aaaacctccg ccgtggttga agagaccagg gaggccgtgg
    12601 acaccacgtc acaacctgtc gcaacagaaa aaaagagtaa gaacaatacc gcattgattc
    12661 tcagcgcggt ggctatcgct attgctctgg cggcgggcgt cggtttgtat ggctggggta
    12721 aacaacaggc cgtcaatcag accgccacca gcgatgccct ggctaaccaa ctgacggcat
    12781 tgcaaaaagc ccaggagagc caaaaagccg agctggaagg cattattaag caacaagctg
    12841 cacagcttaa gcaggcgaat cgtcagcaag aaacgctggc aaaacagttg gatgaagtcc
    12901 aacaaaaggt cgccaccatt tccggcagcg atgctaaaac ctggctgctg gctcaggccg
    12961 attttctggt gaaactcgcc ggacggaagc tgtggagcga tcaggacgtc acgaccgctg
    13021 cagcgttgct gaaaagtgca gatgccagcc tggcggatat gaatgacccg agtctgatta
    13081 ccgttcgtcg ggcaattacc gatgatatcg ccagcctttc tgcagtttcg caggtggatt
    13141 atgacggcat catccttaag cttaatcagc tttcaaatca ggtagataac ctgcgtctgg
    13201 ccgataatga cagcgatggt tcgccgatgg attccgacgg tgaagagctt tccagttcca
    13261 tcagcgaatg gcgtatcaat ctgcaaaaaa gctggcagaa ctttatggac aacttcatta
    13321 cgattcgccg tcgtgatgac accgccgtac cgctgttagc gccaaatcag gatatctatc
    13381 tgcgcgaaaa tattcgctct cgcctgctgg tcgcagcaca agctgtaccg cgtcaccagg
    13441 aagagactta tcgccaggcg ctggagaacg tctccacctg ggtacgtgct tactacgata
    13501 ctgatgatgc caccaccaaa gcgttcctcg acgaggtgga ccagttaagc cagcaaaata
    13561 tctcgatgga tcttccggaa accctgcaaa gccaggcgat gctggaaaaa ctgatgcaga
    13621 ctcgcgtgcg taacctgctg gcacaaccgg cagcagggac aacggaagct aaacctgcac
    13681 ctgcacctgc acctgcacct gcacctgcac ctgcacctgc acctgcacct gcacctgcac
    13741 ctgcaccgca aggtgatact ccggcagccg cgccgcaagg agaataacca tgctaaaagt
    13801 gttattactc tttgttttgc tgattgcggg gatagtggtt ggcccgatga ttgccggtca
    13861 tcagggttat gtgctgatcc agaccgacaa ctacaatatc gaaaccagcg tcacgggcct
    13921 ggcgatcata ttgattctgg cgatggtagt gctgtttgcc attgagtggc tactgcggcg
    13981 gatcttccgc actggcgcgc acacccgtgg gtggtttgtc gggcgtaagc gtcgccgtgc
    14041 acgtaagcag accgaacagg cgctgctgaa actggcggaa ggcgattatc agcaagttga
    14101 aaagctgatg gcgaaaaatg ccgatcacgc tgaacaaccg gtggtgaact atctactggc
    14161 tgccgaagcc gcgcaacaac gtggtgatga agcgcgcgcc aaccaacatc tggaacgcgc
    14221 agcggagctg gccggcaatg acaccattcc ggtagaaatc acccgtgtac gtctgcaact
    14281 ggcccgtaat gaaaaccatg ctgcacgcca cggcgtggat aagctgctgg aagttacgcc
    14341 acgccatccg gaagtattac gtctggcgga acaggcgtat atccgcacag gtgcatggag
    14401 ttctctgctg gatatcatcc catcaatggc gaaagcccat gttggtgatg aagaacatcg
    14461 tgcaatgctg gaacaacagg catggattgg cctgatggat caggcgcgtg ccgataacgg
    14521 tagtgaaggt ttgcgtaact ggtggaaaaa ccaaagccgg aaaacgcgtc atcaagtagc
    14581 gttgcaggtg gcaatggcgg aacatcttat tgaatgtgac gatcatgata ctgcccagca
    14641 aattatcatc gatggcctga aacgccagta cgacgatcgc ctactgctgc cgattcctcg
    14701 actgaaaacc aacaatccgg aacagctgga aaaagtgctg cgccagcaga tcaaaaacgt
    14761 cggcgatcgc ccgctgttgt ggagcacact gggccagtca ctgatgaagc acggagaatg
    14821 gcaggaagca tcgctcgcct tccgcgcagc gctgaaacaa cgtccggacg cctacgatta
    14881 cgcatggctt gccgacgcgc tggacagact gcacaagccg gaagaagctg cagcgatgcg
    14941 tcgcgacggt ttgatgttaa cgttgcagaa caacccacca cagtagttcc ttctcacccg
    15001 gaggcaagca cctccgggac ctttctgatg cataaaaaaa cgcctgctct tattacggag
    15061 caggcgttaa aacaggtctg tatgacaaca agtgggtgct tcactcaacg ttgtgtccat
    15121 ggtgtctgat gaggcataag cgacatctgt cagtggacga taagcaccgt aaacggctct
    15181 gcgtcattcc ggagtttatg aggcactaag gcgaacataa gagatggaat gagcatctac
    15241 tcgtttatta tgccacagag aatcgggaaa taacatccct taacacttgt tatgagataa
    15301 ttctgtaatc ctctttgctt cctgagtaat aacttcctga gtgaatattt aacctgagct
    15361 tgatcctaca catacttatt atgaatgata aaattcattc aattaataac acatatatta
    15421 attgccgtta aaactaaaaa aaagcatcaa taatcaacgc gatataataa acctgcctta
    15481 catatcaact gcgccagagg taggattgaa aacgctctcc tgattttcca attcattttc
    15541 tggataaata aataatttat ttttgtcact attatttatg taatcatcct gtcagggaga
    15601 gggatctcaa ttatcaatgc ttaattacgt catcattttg atgacatgaa accggagcat
    15661 tttgcatgga attttcgttt tcacccaaac ttcttgttgt tgctgtcgcc gccgctcttc
    15721 ctctcatggc cagcgcagca gataccccgt caactgccac cgcacgcaaa ggctttgccg
    15781 gatacgatca cccaaaccag tatccacaga acgctgcaat ttattgaatt tgcacgatca
    15841 tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca accgcaggcg gccacaaaat
    15901 ggccgctatt ttacttattc accaccagca aatgtgcctg cataatgtca ctggccgggc
    15961 gaccgtgcgc cagcaaatca gccattgctt taagatatgg cggtagatgg cggaaataac
    16021 gctgataccc ggcacacaaa taattcagcc ccggtttgcc gctggcatcg agcatgaagc
    16081 ggtgtttcgg gcagcctccc cagcacgctt ttaacacgtt acaactgcga cactgcgccg
    16141 gtaattgctt aaatttatct tcaccaaaca cctgctgttg cggggaatcg atcatttctg
    16201 caattgtttg ctgatgcata ttccccagcc gatattgcgg ataaacatag tgatcgcagg
    16261 cgtaaacgtc gccgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc
    16321 aaaccgcacc cggcgcaccg acaaaattgg caaacgccca ttcgatattc atcacgaaaa
    16381 tcttgccgac gtcgcgtttg atccagtggt caaaaatcgc caccagaaat tcaccaaatt
    16441 catcggggcg cacagaccat tccgtcagtt ccccctgaat atcaccaggc gcatgtagtt
    16501 tcagtccatc gctggcagct gtttcatcag ccaggcgctc gaccaccgga ataaactgga
    16561 tgaattcgac tcccgcatcg cacaaaaaat cataaacctg caacggttgc tgcgcgctgg
    16621 tgcggttgac gcagaccagc acgttatagt cgacatgatg tttttgcagc agcgtcaggg
    16681 cacgcatcac cagcttatgc gttggtctgc cacctttggt cacgcgatat tgattgtgga
    16741 tctcagccgg accgtccagc gataacccaa caagaaaatg attttctgcc agaaatgcac
    16801 accatttatc atcgagcagc acgccgttag tctggaagct gttacttatc ttcctgccag
    16861 caccgtattt cgcctgtaac tttacggcac ggcggtaaaa atccagcccg agtaacgttg
    16921 gttcgccgcc ctgccaggta aaagccactt cgttttgcgg ttcgctggca gcgatatagt
    16981 gacggacata cgcttccagc atgtcatcgt ccatatgcgt gactggcttt tcgcggtaaa
    17041 gggattgttt ttcgagataa aaacagtagt cacagttcag attacaatcg gaaccactcg
    17101 gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc actccttgcc
    17161 tgaagtcatc cttcaatacg ttaagggcga aatcataccg tggggcaatg agttattacg
    17221 ggcgtttatt gcgaagggag ggtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc
    17281 ggggttcaag attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca
    17341 gttgcgctac caagctgcgc tactcgccga aatactgctt tttaaatttt tagttcaatt
    17401 ctttaacgtc gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca
    17461 cctgaagcta gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg
    17521 gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac
    17581 tgagctatag ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc
    17641 gccgggacta aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc
    17701 agcgctctat ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga
    17761 cttcccaccc ctctgtctag tcccttttta aataaattgc gcgtttggtt acggtttgtg
    17821 cgttttgctg cttattcctc cagtttatgc gctttcccgt ggcgattaag gccaaaaact
    17881 ttataaatcg ccgtcaccgc cagcataaag atgatgccaa caaacagcga catacgcgta
    17941 tcttcattaa agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg
    18001 gcccacggga acaggattga gcggaacgga tggctggcaa tcgccgcttt atgtgcacgc
    18061 cgaaaacgca gctggcttat caatatcaca aaccatggca ccatccccgg aagcacgctg
    18121 gcactgtaga cgtagacaaa cacacgctgc ggattgggaa tgatgtagtt caggcatgag
    18181 ccaattagca gaatagcaat agatactgcc acacccgcaa ctggtacgcc gtgacgggaa
    18241 actttcgcca tcgccgccgg taactgacgg tttttcgcca gtgcgtagag catacgtccg
    18301 caactgtaca tgccgctgtt acagccagag agcgcagccg tcagcaccac aaagttgata
    18361 atgcccgccg ctgcggtaat accgattttg gcaaaagtca gtacgaacgg gctgccgttg
    18421 ctgccacttt cattccacgg gaagatggtg acgataacga aaatcgcgcc tacgtagaaa
    18481 atcaggatcc gccacagcac tttgcctacg gcactgcgca acgtcacctg cggattcttc
    18541 gcttcaccgg cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac
    18601 agcgcggtca ggaacccttt ccagccaccc gcaaagaaac cgccatgctc tgtgagattg
    18661 ctaaaaccaa tcgactgccc gccattgcca aagccaaaga aaatcacgcc caggccaatg
    18721 acaatcatca cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg
    18781 tacaaccgca ccgccgccag attcgccaac gccaccagcg ccactgcgat caatgcgggt
    18841 atccactgcg ccatctccgg gaaccagaac tggacataaa cgccaatggc ggtgatttct
    18901 gagatcccca ccgccatcca cataaaccaa taagaccagg cggtgagata accaaagaac
    18961 gggctcatat aacgatgcgc ataaacggcg aacgaaccgg taaccggttc gaggaacaac
    19021 atttcgccca ttgaacgcat gatgaaaaag acgaatagcc cggcgatgat ataggccaac
    19081 aatacggatg gcccggccca tttcaaggta ctggcggccc ccataaacag gccgacgcca
    19141 atggtgcccc ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct
    19201 ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt
    19261 atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata
    19321 aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac ggagtgacca
    19381 ctccgtcgct ttacaaagag aggaaaatca gaggttgccg gtgtagtgcc agcgtaaata
    19441 acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca
    19501 ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg tgaaaacatc
    19561 gtaagtcccg ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat
    19621 gaagatctcc tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg
    19681 aatgcgttca aacagcgcct gacgctgctc gggtttaaaa taaccatcct gactgccaac
    19741 gatattcaca ttccactggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt
    19801 accgcccaca agaaataccg gcgtcccttc tttgcctgcg cgcgccatca gctcttccca
    19861 gagatcggca ccggcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg
    19921 tacaacgctg atgccatccg catatttaaa ttcggcagcg ttaattaact ccctgacctc
    19981 ggcgttatct tcaatggtca gcattttttc agcattaatg gcaaccagcg ttccctgctt
    20041 aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc atatcacgcc aaccaatcaa
    20101 ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg ttattcattg ttatccttca
    20161 acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga gtccggcgct ttcaaaaagc
    20221 cagtacaaca gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagactacg
    20281 cgtgagacaa acgaatccag cccttcacgt gccagcacga tcatattgaa gatcgcccca
    20341 aagcagaaac tgtgcaatat cgcagcctta tagcgattag gctcgcggtt gcccagctca
    20401 tacagccagt cgaaccattt gatgatcaga ccaaccacga tcgccccgag cgggatgaac
    20461 aacgcgccgc ccatcaccac cagtgagcct ataagcgtag gcgagatcgc cagtccggag
    20521 tggttattca gcacttccct ggtaaagtag ttggctgagt tcagcaccat actcgggcga
    20581 cccggccaca gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc
    20641 tggaagtcga tgttgtcgta gttctgcaac agcaacgcca gattctccca cggcgagaag
    20701 gtgtcgcgag tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca
    20761 tagcgtttta gcgccagcca gaacatgccc acaatcccca gcacgcccgc cgccgccagc
    20821 atccacaacg aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg
    20881 atgatattgg cgcgagtgcc gccgacaatc atataagtca gcaagccaaa ggcgaccgtg
    20941 ctgacgagga aaaacagcca cgctttgcta tcctggcgca gaaagtagac caccagcatc
    21001 gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc actggagaag
    21061 atctgactgt aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg
    21121 acgcttacca gcgcgatgcc catcaggatc acccacgtaa gattggtctc cacgcggttc
    21181 atggtaaaca gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg ggttttgtag
    21241 gtgacatagt aaaccgcgta gaagcagccc gcagaaagca acgcctgcaa caagatttct
    21301 ggcggcgcga caccaacatc aaagcgaaat accagcacgc tggtcagcgg gaagccgaag
    21361 aaaaaggtga gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga
    21421 aactcaaacc aagtcagcgt ggcaataaac agcgtgcaga gcagccagac cacaaacagg
    21481 ccactgaatt gcagcagact catgcgacct ccccggcggc aatcgccaac gcccgctgcc
    21541 agccttgtag atagttaggg ctaaagaagg caatggtgtt tttatccacc gacgccaact
    21601 ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc ggtagtaaac agcaccggca
    21661 aatgctgttc cgtcatatcc tgccagaacg gattttcccg gttaagcaca caaggaatgc
    21721 ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg gcgggcaaaa ataaagtaac
    21781 caagatcgca ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt tcgctcaaaa
    21841 tttgcagatt ttcttcgctg aataactcca gccccgcctg acgaacttcc tcaatgtacg
    21901 cttcgttatt aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct
    21961 gatgaacggc gcgcaaggca gcaatatggt cattgctgcg gtcaccggag ttccccacca
    22021 gaatggtcat tttcccttca cgttgccgat cgttcgccat cgtattgaga gaagggtcca
    22081 tccgcgtcgg gaagtacagc agttcgcccc gcacctttgg atgcgttttg gcaaaaaagc
    22141 tcaaatcacc gcgggtggca aatacacagc cgactcgctt ttgcgccagg cgacgtagcg
    22201 ggtaaaaaag cttatatctc aagccactgg aaagctcata caggtctgcc ccccagatat
    22261 gccagaaaaa ctggccgggc ttaatgccac cactcagcag agccagccac agtgtgggat
    22321 tgaactgacc gtggaaaaaa aatcgctgct gacggttagc ttttgctttc gcgatgaccg
    22381 cttccgccag cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac
    22441 ttaagccgtc gtccttgcca acaaccataa actcgcgcgc gtgctcgctc gtcgcggcca
    22501 gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg agggatatcc gatcccagta
    22561 cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga
    22621 aaaatagacg atataagttg ccatatatgc ctgcgccgcg cccagtgcac catgcgcagg
    22681 gattagccag tgggcaaata ccatcaataa agtgaactgg ctgacttccg ccagaatata
    22741 aaaccgcagt gacgctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa
    22801 cacatcaccc actaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa
    22861 cagcaaccag atagcaaaat cacgcagcaa ccagacggta aaactcgccg ccgccactgc
    22921 cggtaagacg aattttagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag
    22981 ccgcgacaac gtgggcagca aatatacgct gaacgatgcc gtaatgaatt gcaggtaggc
    23041 atcggaaata ctgctcaccc cttgccagat ccccacttca tcccagctat actgcgccgc
    23101 cagcagttta cgcatcatga tgtaagcaac aggcaaggtc accgacgtaa tcaacgccat
    23161 gagcgtaaat ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct
    23221 taacgggatg acaccacgtt tgatcaacat gatggcggca ggaattacca ccagcgcggg
    23281 aatcagcgcc agacccagca acgccccttc ataaccgccc aaacggtaac tgacgtagta
    23341 cgcgagaacg ccaatcaagc tgccgacaat cagcgataac gcattacctg cggcatcgcg
    23401 aaagcctttc atcagcgcca gtaacaggtt gccccaggcg atccccattt gcaccagcgc
    23461 cactaaacgc accagcccct gatagtcggt attaccaaac aatccctggc tgattggcgc
    23521 agctgccagc acaaaaacca gcgccatcag cgtagagaaa ccaagtacca tcgctgatga
    23581 agtgccgacc acgcggcgca gctgttgcgg attatcatgg tactgggcaa cgtatttggt
    23641 tacaccgtta aagatgccag ccccggcaag cacgccgagc acggtaatca actggcggaa
    23701 atttgccgcc agcccaagcc ccgccggacc aaatgacacc gccagcaact taccgaccag
    23761 taacccggca ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa
    23821 cgacatatca ggagaagtag ttcaacaaag tcgcaattac cgtacgctga ttgacgggcg
    23881 acaggttgta gaacagcggc aggcgcagca ggcgctcgct ctctttggtg gtgtagcgat
    23941 cttcaccgtg gaactcacca aagtgttccc ccgcagggca accgtgcagc ggaatgtaat
    24001 gaaacaccgc catgatttcc gcttctttca gaaagttaat caacgcgctc cggtcatcaa
    24061 tatcccgcag tttaatgtag aatatatgcg cgttctgcac gcagccatcg ggaatcgacg
    24121 gcagctcgat acgcccggct ttcgccagag gcgctaacgc atcgtagtag ttttgccaca
    24181 gcgccagacg ttgctggttg atacgatccg ctgcttccag ttgcgcccac aggtatgcag
    24241 cttgcagatc ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct
    24301 gaccacggaa gaactggctg cggttagtgc ccttttcacg gatgatctcg gctcgttcga
    24361 ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc acccgccgtg tagtttttgg
    24421 tttcatggaa gctaaagcag ccaatatgac caatggttcc cagtgcacgc cctttgtaag
    24481 tggacatcac gccctgagcg gcatcttcca ccacaaacaa attatgcttt ttcgccaacg
    24541 ccataatggt gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg
    24601 ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat gttcatggtg tccgggcgaa
    24661 catccacaaa gacgattttc gcgccacgca gcacaaaggc attggcggtg gagacaaagg
    24721 tgtagctcgg catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct
    24781 ccagcgaagc ggtgcaggac ggcgtcagta acactttggc gctgccaaaa cgttgctcca
    24841 gccactgctg gcagcgacgg gtaaaaccgc catcgccaca cagtttgccg ctacccattg
    24901 ccgactgcat atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt
    24961 gatcacctgt ataaccagta cgcggtgctt tctacattcg caccactttg tatgtatcgt
    25021 ttaagcgcgg cggtgttgcc catttgggtc gccacccgca aagttgtttt accgcgagca
    25081 tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg caccgcgtcc agccagcagg
    25141 ccaattcgcg catctgtcgc attgagttcc cgtaaagaga catagccgcg aatatcgccg
    25201 gacgccgcac gtagaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc
    25261 cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc acggcgcacg aaaacggctt
    25321 tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa tatcggtctc ttgtgccacg
    25381 acagcaccgc tatcactgac attgttcacg ggtagcgcca aatcaacttc accttctacc
    25441 agggagaatc ccagctgttg cagggcatcc agttcacccg tatttgatgc cgcaattttg
    25501 gcctgcaccc gtgaccacgg cgctaacgcg tctggcgtca ggagcggtgc ttcagacgta
    25561 atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata
    25621 ctggcgcgga cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgctaa
    25681 tgaactggca gcacgcttca caccctcgtc atcgagccag ccgttacgcc aggcgatctc
    25741 ttccaggcag gcaatcttaa agccctggcg tttttctact gtctgcacaa aggtactggc
    25801 ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca aatccgcgcc cgagcagttc
    25861 aacggtcagg ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc
    25921 acgctccgac ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag
    25981 cccggtcacc gcccagtttg acttcggctg ttttggcttt tcttccagcg agatagcgcg
    26041 gaaattgtcg tcaaactcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa
    26101 taccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc ttcggactga agccctgacc
    26161 gaagaagatg ttatcgccca gcaccagaca agaaggttca ccgttgagga aggtttcacc
    26221 gatgataaag gcctgcgcca gaccgtccgg gctgggctgt tcggcgtatt ccagctggat
    26281 accgaactca ctaccatcgc ccagcaggcg ctggaaataa cctttatctt ccggcgtagt
    26341 gatgatgaga atttcgcgga taccggccaa catcagcacc gacagcggat agtaaatcat
    26401 tggcttatcg taaatcggca acagttgctt cgatacgccg cgcgtaatcg gatgcaatcg
    26461 ggtgccggaa ccgcccgcca ggataatacc tttcatgttt gcctccgctg aaaattagcc
    26521 tttcagacct aaacgctcgc cctgatagct gccgtcctgc acctgcttcc accagctttc
    26581 attagccaga taccactgca ccgttttacg cattccactt tcaaaggttt cctgtggcag
    26641 ccagccaagt tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg
    26701 acggtcagcg acaaaggtga tcaagtcacg atagtgcacc acgccgtgcg gcttgttcgg
    26761 agccagttct tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt
    26821 gtgaccacca atattatagg tttcaccgac tttcccggtg gtcgccacgc aatacagcgc
    26881 gcgggcgtga tcttccacat acagccagtc acggatttgc tgcccgttgc catataccgg
    26941 cagcggttta cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg
    27001 gtaaggaccg tagttattcg agcagttggt gataagcgtc ggcagaccgt aggtccgcag
    27061 ccaggcgcgc accagatggt cgctgctggc ttttgacgcg gaataagggc tgctcggcgc
    27121 atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc
    27181 ggtggagata tgatgaaaac ggaacgctga ttttttatct tccgtcagcg tattccagta
    27241 agcccgcgcc gcttcaagca atgtataagt cccgacaatg ttggtttcaa taaacgctgc
    27301 cgggccgtca atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg
    27361 ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg cagatatcaa ctttctcaaa
    27421 ggcaaagcga tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg cgtaggtcag
    27481 cttatcgacc actaccaccg cgtcgctcgt ttcgttgatg atataacgca ccagcgccga
    27541 gccaataaac ccggcaccac ctgttatcag aatttttctc atcagcgcca gactcctttg
    27601 gcatcgacga catactgctg atggacattg tcgccattga taactttaaa ctgactatga
    27661 tcgaccagca tcaccagcac gtctgccgtt gccagcgcct cgtcaagctg cgccagagta
    27721 caaagcccgg tcagtttttt cggcaactgg tggatgttag gctcaacaac cagagtttcg
    27781 ccgctatgcc actgggcgat cagttcagcg atttccatcg ccgggctttc gcgcaggtca
    27841 tcaatattcg gtttaaacgc cagaccaaag caggcgattt tcagttcact ggcgcgttta
    27901 tcggtagccg ccaggcaatc agccaccgcc gctttcacct gatcgataac ccagaacggt
    27961 ttgtgatcgt tcacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc
    28021 acgataaacc acggatcaac agcaatgcag tgaccgccca cgccagggcc aggctgaaga
    28081 atattaacgc gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga
    28141 tcggcacaaa tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt
    28201 tcggtgagct tacacatttc cgccgtccgc gagttagtga cgacacactc accttcgagg
    28261 aaaattttgt acagttcgct ggcgcgggtc gaacaaaccg gcgtcatacc accaatcacg
    28321 cgatcgtttt taatcagctc gaccataacc tggcccggta acacgcgttc cgggcagtaa
    28381 gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga aagtgagatc cggacgcatc
    28441 tctgctaacc attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc
    28501 accagcgcgc cttttttcag cactggcgca atggagcgag cagccgattc aacgtaggtc
    28561 atatctggct catgatcgcc tttaaacggc gtgggtacag caatcagcca ggcatccgct
    28621 tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc
    28681 gccaaatcag gctcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg
    28741 ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa acgctgctgc tgtaggcagc
    28801 ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat
    28861 tttttaacgc ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc
    28921 ggctcatagt ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc
    28981 gctgcttatc cgtgcctacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag
    29041 tggtatcgcg catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc
    29101 ctgagtcggt caaaatcagc caggcgtggt tcatcaacca gacaaacggt aaatactcct
    29161 ggggatcgat cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga
    29221 cgttcgggtt gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcaatgt
    29281 ctgccaacgc gtggcagatt tcttcaaaac cacgaccgaa actctcacgc ctgtgaccgg
    29341 tcaccagaat catcttttta tcggggtcga taaacgggta atttgccgcc agttctgaac
    29401 gcagcgtgtc gctgctcatc acctgatcac gcacccataa cagtgcatca atgactgtat
    29461 taccagtaat gaagattcgg ctatccgcaa cgttttcacg cagcaagttt tgccgggaag
    29521 tttcggttgg agagaagtga tacatcgcca gatgcccggt caatgtacgg ttagcctctt
    29581 ccggccacgg cgaatagaga tcgcccgtgc gtaaaccagc ctcaacgtga ccaacaggaa
    29641 tacgctgata aaacgccgcc aggctggttg ccagcgtcgt tgttgtgtcg ccgtgaacca
    29701 gcacgacgtc tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg
    29761 ttatctctgt caggccctgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg
    29821 aaaagagttt cagcacctga tcgagcatct cccgatgctg cgcagtgacg caaactttag
    29881 cctcaaaaaa aggagctttt gccaacgcat gcaccaacgg agccatcttg atggcttccg
    29941 ggcgcgtacc aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagcg
    30001 cctttgcgct cgccgcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca
    30061 gccccgatca gccccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta
    30121 tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc
    30181 agggttggac caacattcag ggtgtttaac atggcccgat tctgatcata gtcgagatca
    30241 aaggccggac cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc
    30301 aggaacattt ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata
    30361 ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt tcatccggcg gtcgtagatg
    30421 gctttcgcca cctcttcctg acgcttcacc tgagctttca tctggatggt gcgtgccgcc
    30481 catgcgcctt tcagctcatc attcagatgg ctggctgcac gctggctggc aaaagcaaca
    30541 tactgacgta acaggttatt agcgtcaggc gcggtttcgg caataagctt cacgctgtca
    30601 ttgaccgcgc gggtaaagtc tccggggata aactggatgt tgttaatcat ttcatccagc
    30661 aacgccgcat cggctttgct gttgcccacc atccgctgtt tgtaatagtc ggtttgcagc
    30721 cagaactctc tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataggcttcg
    30781 tccatgaccg atggttgatc ggcagaagcc atgtttgaac ggacatccag gttacgcaaa
    30841 aattgctgct gcgagtaata tccccccagc atattcaccg ttggacgatc ggtaatcgcc
    30901 gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca gcgcgattaa cgcaaacgcc
    30961 agccccatgc caataatcca tagcttccca gcccacaagg tacgaaacaa cccacgaata
    31021 tccagttcat tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc
    31081 tcatttattt ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc
    31141 gcttaataaa gcgagcaact ttccaggcac gcttaatgca atatccatag aggaagaatg
    31201 ctagcaaaaa gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca
    31261 cgccaatgga agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc tgacgggaag
    31321 taaacccggc acgcatgatc aaatggtgaa tatgctgacg gtcaggagag aatgggctca
    31381 tgcctttacg caggcgacgg tacataatcg ccaccatatc cattagtgga atggcgatta
    31441 tccacaaagc ggtaaccggg ctgatgggat gggttttgcc ctgggtcgtt tcgagcagga
    31501 tccagataac ggtaaaacca atcagcgtac tgcccgcatc acccataaag actttgtagc
    31561 ggcgacccag gataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa
    31621 agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa aatcataccg attgctgcaa
    31681 acgagacgca ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat
    31741 taatggccgc ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct
    31801 cccaggagcc aaagatataa cccaggctac tgagataaag tttgccgaac accatcataa
    31861 caatgccaac agcggcctgt atggtggcac ggatttttac gctgatatca aaacggtcat
    31921 ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag atagagagat gcatgcggaa
    31981 tatagtaatc gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa
    32041 cgagaggtat caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt
    32101 tttttgccac cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga
    32161 gatcagtact cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga
    32221 agtataacca cgaagacctt tattcagaag ggaaagtccg ttccgaaccc accagcactg
    32281 tgcaaaaatt aaccatttga tatgcaaatt gcataacatg ccagcaaatt ccaggaattt
    32341 tcctaacatc gccagcgcgg cattaagatt acagatataa aagcaaaacg ccacgtaaac
    32401 acgtggcgtt tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc
    32461 attggtcttg gtcatggcca gtttattaat gaggaattcc attgcatcga tttcgcccat
    32521 cgggtgaatg attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag
    32581 ctcttcttta cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc
    32641 gatcttacga gagaggtgca gttccatgtt gcctgtacct ttaaactctt cgtaaataac
    32701 ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc
    32761 ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga cgatgaaggg cattggcgtc
    32821 cacaccaccg gtcaacactt tacctgacgc cggaacaaca gtgttgtaag cgcgcgccag
    32881 acgagtgatg gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttggc
    32941 cttctcgatc accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga
    33001 agcaacaact tcacctttta ccagacgctg catctcggtt acttcttccg gacgttcgtc
    33061 gatcagcaga accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt
    33121 ctgcagcagc atagttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg
    33181 accgataggt gatgccagat ccagtacgcg agcggttaaa tcttccgtag aaccgttacc
    33241 acgttccata cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt
    33301 gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata
    33361 gcgttcacct tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt
    33421 gaaacggcgg atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct
    33481 gtctgcggaa cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa
    33541 gatatcttcg ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg
    33601 catacgagcc aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg
    33661 cgtattcttt aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct
    33721 cgaacttaat gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact
    33781 catgtctttt cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt
    33841 gagtgacgag ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa
    33901 ttacaagatt caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga
    33961 ctcacgacgg gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc
    34021 aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac tttggttgcc
    34081 gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc
    34141 gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg
    34201 tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag
    34261 aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc
    34321 aggtgaataa ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt
    34381 cgttgttggt gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg
    34441 taaagcaata gtaagctgat attctaccac actatgagca aaacacattt aacagaacag
    34501 aagttttccg acttcgccct gcatccgaag gttgtagaag tccttgaaaa aaaagggttt
    34561 cataactgta cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta
    34621 gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat
    34681 tatcttctct ctcatcctgc gattgccgat cgcaaggtga atcaaccacg tgccttaatt
    34741 atggcaccga cgcgtgaact tgccgtgcag atccatgccg acgcagaacc gctggcggaa
    34801 gctaccggcc tgaagctggg tctggcttac ggtggtgatg gctacgacaa acagctgaaa
    34861 gtgctggaaa gcggcgttga cattctgatt ggcactacgg ggcgtttaat tgactacgcc
    34921 aagcagaacc acattaacct cggtgccatt caggtggtgg tactggacga agccgatcgc
    34981 atgtacgatc tgggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca
    35041 aaccagcgcc tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg
    35101 ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac
    35161 cgtattaaag aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg
    35221 ctgatcgaag aagagtggcc agacagagcg attattttcg ccaataccaa acaccgttgt
    35281 gaagagatct ggggccacct ggcggcagat ggtcatcgtg tcggtttgtt gacaggcgat
    35341 gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta cccgtggcga tctggatatt
    35401 ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc cggcagtgac gcacgtcttt
    35461 aactacgatt tacccgatga ctgtgaagat tacgttcacc gtattggtcg tacaggtcgc
    35521 gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag agtatgcatt gaatttgcct
    35581 gctattgaga cctatattgg tcactcaatt ccggtaagca aatacaatcc ggacgcattg
    35641 atgaccgatc tgccaaaacc gctgcgcctc acgcgcccac gtacaggcaa tggtccgcgt
    35701 cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaatgtca tactcaaaat
    35761 cattcaaact gtatcaagat ggcaagagaa tgaatcccga tgagcatact gtagtaagtg
    35821 attcaggtga aagaatgcag ccaacacaga gacagattga aggatgaaga gtatgggttc
    35881 cacctcgtcg ctgtatgcag ccattgatct cggttcgaat agttttcata tgctggttgt
    35941 gcgcgaggtg gctggaagca tccagacgct gacgcgaata aaacgcaaag tgcgtctggc
    36001 tgctggcctg aatagcgaaa atgccctgtc taatgaagca atggagcgcg gttggcaatg
    36061 tctgcgcctg tttgctgaac gtctgcaaga tatccctccc tcgcaaattc gcgttgtcgc
    36121 tacggcgaca ttacgtcttg ccgtcaatgc gggtgatttt attgccaaag cgcaggaaat
    36181 cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa gcacgtctga tttatcaggg
    36241 cgttgctcac acaactggcg gtgccgatca gcgcctggtg gtggatatag gcggtgccag
    36301 tactgaactg gtaaccggca cgggtgcaca aaccacctcg ttgttcagcc tgtcgatggg
    36361 ctgcgtcacc tggctggaac gctattttgc cgatcgtaat ctggggcagg aaaattttga
    36421 tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt gccgatgaat tacggtatca
    36481 cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag gcattacagg aaatcatgat
    36541 ggcacagggg atggatgaac gcattaccct ggaaaagttg cagcaattga aacagcgagc
    36601 cattcattgc ggtcggctgg aggaactgga gattgacggg ctgacgctgg aacgtgcgtt
    36661 agtgttcccg agtggtctgg cgatcctgat cgccattttt accgaactga atattcagtg
    36721 tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc tacggcatgt tgcatctggc
    36781 cgtagaccag gatattcgca gccgtacgct gcgtaatatt cagcgccgct ttatgatcga
    36841 tattgatcag gcgcagcgcg tagccaaagt tgcggctaac ttcttcgatc aggtggaaaa
    36901 ggaatggcat cttgaagcaa taagccgcga tttgctcatc agcgcctgtc agcttcatga
    36961 aatcggcctg agcgttgact ttaaacaagc gccgcaacac gctgcttatc tggtgcgtaa
    37021 tctggatctt cccggtttta cccccgcaca gaaaaaattg ctggcgacgc tactgctcaa
    37081 ccagactaat ccggtcgatc tctcatcgct gcatcagcaa aatgccgtac cgccgcgcgt
    37141 cgcagaacaa ctctgccgtt tactccgcct ggccatcatt tttgccagcc gtcgccgtga
    37201 cgatctcgtg ccagagatga cattacaggc taaccatgaa ctgttgacct tgacgcttcc
    37261 gcaaggttgg ctaacccaac atccgctggg taaagagatt attgctcagg aaagccagtg
    37321 gcaaagctat gtccactggc cgctggaagt gcattaatga gtaagtgccg gatgcgatgc
    37381 tgacgcatct tttccggcct tgattatttc cctcgttttg ccgccatcat cgctttcaga
    37441 ttcgccagat ggctttgccc tttctgcatc cgttcttcgg cgctgaccac tttgcgctcc
    37501 tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa agcggctcgg ctccgggcgc
    37561 accagctcgc cgtactgacg gcgttcttta cacagcgtaa aggtcaattc cttctgggcg
    37621 cgggtaatgc cgacataggc cagccgccgc tcctcatcga tattatcttc atcgatgctg
    37681 ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt agacataagg aaactccagc
    37741 ccttttgacg cgtgcagagt catcagttgc acctgatcca gctcttcttc actctcacca
    37801 cgctccatca tgtcgcgcaa agtaaagcgc gtcaccacct gggtgagcgt catcggctca
    37861 tccagttcgc tgccttccag catctccgtc atccagctaa acagttggtt gacgttcttc
    37921 atgcgcattt cggcggcttt cgggctgggc gatgtttcgt acagccagga ttcataatcc
    37981 atgccgtgga tcagatcgcg caccgcggca atcggctccc gctccgccag acgctggatt
    38041 tctgccaacc agtgagtaaa gcgggtcaat gcttcataac cacgtccgct aagcgtctga
    38101 ctcaggccca tatcaaagct ggcggtaaac atgcttttat tgcgcgtcat cgcccactca
    38161 cccagctttt tcagcgtcgc cgggccaatc tctctcttcg gcgtgttaac gatacgcaga
    38221 aatgcgctgt catcgtccgg gttagtcagc acgcgcagat aagccagcaa gtccttgatt
    38281 tcaggacgag agaaaaacga cgtaccacca gaaattttgt acgggatgcg gttttgcatc
    38341 aggaattttt caaacactcg cgactgatgg ttaccgcgat aaagaatagc gtaatatttg
    38401 tactgcgttt tattgacgaa gtgatgcgca atcagttcac cagtgacgcg ctcggcttca
    38461 tgttcttcgt tattcgcgct taaaacttta agctctgtgc cgtaacccag ttcggagaac
    38521 agacgcttct caaagacgtg cgggttattg gcgatcagga tgttcgccgc tttcaaaata
    38581 cgtccggaag agcggtagtt ctgctcaagc ttaatcacct tcagcgccgg aaaatcctga
    38641 ctcagcagca ccaggttttg tggacgtgcg ccgcgccagg agtagatcga ctggtcgtca
    38701 tcgcccacca cggtaaagcg cgcgcggctg cccaccagca gtttcaccag ttcatactgg
    38761 ctggtgttgg tgtcctgata ctcatccacc agcagatagc gaattttgtt ctgccagcgc
    38821 tcgcggactt cttcattgcg ttgcagcagc aacgtcggca ataaaatcag atcatcgaag
    38881 tcgagaacat tgcacgcttt caggtgtgca tcatacagcc cataacaatg ggcaaaaata
    38941 cggtcccgct cgccaatcgc actggctgcc gcctgggacg gtgttttgag atcgttcttc
    39001 cagttagaga tggtcgaaat caactgttgc aggagaactt tgtcatcttc aatcagccct
    39061 tcggtcaact ctttgagcaa cgccagctga tcggtatcgt caaacagcga aaagttcgcc
    39121 ttcatcccca gcgccgcata ctcacgcttg atgatatcca gccccagcgt gtggaaggtt
    39181 gagatcatca gcccacgcgc ctctttgcga cccagcgtct gtccgacacg ctctttcatc
    39241 tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat gtcgcgcctg ataaccgcaa
    39301 ccgcggatca gatgggcgat tttattggtg atcacacgag ttttaccgga acccgcgccc
    39361 gccagcacca ggcaggggcc ggtaacgaat tcgacagctt gttgttggcc ggggtttaga
    39421 cgcataggtg tattgctcaa tcttcgaacg gggggaggat tgtagcagaa tcgcatttga
    39481 atgtgaccga gtgtaagccg ctaaccggtt ggctgcggat gtttaaatca gagagggaag
    39541 accgaaaccg gagttcatcg tgaatccatt agacttagaa aatatcgggt cgtaacgttg
    39601 tgttattact ttttcagagt ttactaagga gaggtgtact cgtggcgatt aatttcagtc
    39661 ctaaggttgg tgaaatactg gaatgcaact ttggtaacta tcctgttagc cagaatggac
    39721 agttcagcac cacgtattac gatggccgca ttcctcctga aatgataaaa aacaggtta
//
LOCUS       DAJTMK010000015        39591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTMK010000015 DAJTMK010000000
VERSION     DAJTMK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39591
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.015"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..351)
                     /locus_tag="OO135_000668"
     CDS             complement(<1..351)
                     /locus_tag="OO135_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiE"
                     /protein_id="HCR5396618.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLC"
     gene            complement(810..896)
                     /locus_tag="OO135_000669"
     tRNA            complement(810..896)
                     /locus_tag="OO135_000669"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(860..862),aa:Leu,seq:gag)
     gene            complement(911..1243)
                     /gene="secG"
                     /locus_tag="OO135_000670"
     CDS             complement(911..1243)
                     /gene="secG"
                     /locus_tag="OO135_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5396619.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1471..2808)
                     /gene="glmM"
                     /locus_tag="OO135_000671"
     CDS             complement(1471..2808)
                     /gene="glmM"
                     /locus_tag="OO135_000671"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5396620.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2801..3649)
                     /gene="folP"
                     /locus_tag="OO135_000672"
     CDS             complement(2801..3649)
                     /gene="folP"
                     /locus_tag="OO135_000672"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5396621.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3739..5673)
                     /gene="ftsH"
                     /locus_tag="OO135_000673"
     CDS             complement(3739..5673)
                     /gene="ftsH"
                     /locus_tag="OO135_000673"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5396622.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPSFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGTSNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5773..6402)
                     /gene="rlmE"
                     /locus_tag="OO135_000674"
     CDS             complement(5773..6402)
                     /gene="rlmE"
                     /locus_tag="OO135_000674"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5396623.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6528..6821
                     /gene="yhbY"
                     /locus_tag="OO135_000675"
     CDS             6528..6821
                     /gene="yhbY"
                     /locus_tag="OO135_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5396624.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6978..7454)
                     /gene="greA"
                     /locus_tag="OO135_000676"
     CDS             complement(6978..7454)
                     /gene="greA"
                     /locus_tag="OO135_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5396625.1"
                     /translation="MQTIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7702..9135
                     /gene="dacB"
                     /locus_tag="OO135_000677"
     CDS             7702..9135
                     /gene="dacB"
                     /locus_tag="OO135_000677"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5396626.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9175..10347)
                     /gene="cgtA"
                     /locus_tag="OO135_000678"
     CDS             complement(9175..10347)
                     /gene="cgtA"
                     /locus_tag="OO135_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5396627.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWIEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(10363..11328)
                     /locus_tag="OO135_000679"
     CDS             complement(10363..11328)
                     /locus_tag="OO135_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5396628.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSASLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYSLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11455..11712)
                     /gene="rpmA"
                     /locus_tag="OO135_000680"
     CDS             complement(11455..11712)
                     /gene="rpmA"
                     /locus_tag="OO135_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5396629.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNHKFISIEAE"
     gene            complement(11733..12044)
                     /gene="rplU"
                     /locus_tag="OO135_000681"
     CDS             complement(11733..12044)
                     /gene="rplU"
                     /locus_tag="OO135_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5396630.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12303..13274
                     /gene="ispB"
                     /locus_tag="OO135_000682"
     CDS             12303..13274
                     /gene="ispB"
                     /locus_tag="OO135_000682"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5396631.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13501..13779
                     /gene="sfsB"
                     /locus_tag="OO135_000683"
     CDS             13501..13779
                     /gene="sfsB"
                     /locus_tag="OO135_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5396632.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13827..15086)
                     /gene="murA"
                     /locus_tag="OO135_000684"
     CDS             complement(13827..15086)
                     /gene="murA"
                     /locus_tag="OO135_000684"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5396633.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(15141..15395)
                     /gene="ibaG"
                     /locus_tag="OO135_000685"
     CDS             complement(15141..15395)
                     /gene="ibaG"
                     /locus_tag="OO135_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5396634.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15555..15848)
                     /gene="mlaB"
                     /locus_tag="OO135_000686"
     CDS             complement(15555..15848)
                     /gene="mlaB"
                     /locus_tag="OO135_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5396635.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15848..16483)
                     /gene="mlaC"
                     /locus_tag="OO135_000687"
     CDS             complement(15848..16483)
                     /gene="mlaC"
                     /locus_tag="OO135_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5396636.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16502..17053)
                     /gene="mlaD"
                     /locus_tag="OO135_000688"
     CDS             complement(16502..17053)
                     /gene="mlaD"
                     /locus_tag="OO135_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5396637.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17058..17840)
                     /gene="mlaE"
                     /locus_tag="OO135_000689"
     CDS             complement(17058..17840)
                     /gene="mlaE"
                     /locus_tag="OO135_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5396638.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17848..18657)
                     /gene="mlaF"
                     /locus_tag="OO135_000690"
     CDS             complement(17848..18657)
                     /gene="mlaF"
                     /locus_tag="OO135_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5396639.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18867..19844
                     /locus_tag="OO135_000691"
     CDS             18867..19844
                     /locus_tag="OO135_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5396640.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKFARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19858..20844
                     /gene="kdsD"
                     /locus_tag="OO135_000692"
     CDS             19858..20844
                     /gene="kdsD"
                     /locus_tag="OO135_000692"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5396641.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSTTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20865..21431
                     /gene="kdsC"
                     /locus_tag="OO135_000693"
     CDS             20865..21431
                     /gene="kdsC"
                     /locus_tag="OO135_000693"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5396642.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21428..22003
                     /gene="lptC"
                     /locus_tag="OO135_000694"
     CDS             21428..22003
                     /gene="lptC"
                     /locus_tag="OO135_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5396643.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21972..22529
                     /gene="lptA"
                     /locus_tag="OO135_000695"
     CDS             21972..22529
                     /gene="lptA"
                     /locus_tag="OO135_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5396644.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22536..23261
                     /gene="lptB"
                     /locus_tag="OO135_000696"
     CDS             22536..23261
                     /gene="lptB"
                     /locus_tag="OO135_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5396645.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRHLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23309..24742
                     /gene="rpoN"
                     /locus_tag="OO135_000697"
     CDS             23309..24742
                     /gene="rpoN"
                     /locus_tag="OO135_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5396646.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24765..25052
                     /gene="hpf"
                     /locus_tag="OO135_000698"
     CDS             24765..25052
                     /gene="hpf"
                     /locus_tag="OO135_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5396647.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25170..25661
                     /gene="ptsN"
                     /locus_tag="OO135_000699"
     CDS             25170..25661
                     /gene="ptsN"
                     /locus_tag="OO135_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5396648.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25707..26561
                     /gene="rapZ"
                     /locus_tag="OO135_000700"
     CDS             25707..26561
                     /gene="rapZ"
                     /locus_tag="OO135_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5396649.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26558..26830
                     /gene="npr"
                     /locus_tag="OO135_000701"
     CDS             26558..26830
                     /gene="npr"
                     /locus_tag="OO135_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5396650.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27044..27676
                     /gene="yrbL"
                     /locus_tag="OO135_000702"
     CDS             27044..27676
                     /gene="yrbL"
                     /locus_tag="OO135_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5396651.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27673..28401)
                     /gene="mtgA"
                     /locus_tag="OO135_000703"
     CDS             complement(27673..28401)
                     /gene="mtgA"
                     /locus_tag="OO135_000703"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5396652.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(28398..29051)
                     /gene="elbB"
                     /locus_tag="OO135_000704"
     CDS             complement(28398..29051)
                     /gene="elbB"
                     /locus_tag="OO135_000704"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5396653.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADATELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29281..31617)
                     /gene="arcB"
                     /locus_tag="OO135_000705"
     CDS             complement(29281..31617)
                     /gene="arcB"
                     /locus_tag="OO135_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5396654.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDILMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31713..32642)
                     /locus_tag="OO135_000706"
     CDS             complement(31713..32642)
                     /locus_tag="OO135_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5396655.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLEHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            33316..37776
                     /gene="gltB"
                     /locus_tag="OO135_000707"
     CDS             33316..37776
                     /gene="gltB"
                     /locus_tag="OO135_000707"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5396656.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRTGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37789..39207
                     /gene="gltD"
                     /locus_tag="OO135_000708"
     CDS             37789..39207
                     /gene="gltD"
                     /locus_tag="OO135_000708"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5396657.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            39391..>39591
                     /locus_tag="OO135_000709"
     CDS             39391..>39591
                     /locus_tag="OO135_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396658.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLI"
BASE COUNT         9775 a        10737 c         9806 g         9273 t
ORIGIN      
        1 acatagataa gggatataca tccccagcat tttgacaact tcatccggat cactctggag
       61 cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg aatagaggct
      121 aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca ggcgaagcgg
      181 aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca atcgctgaac
      241 catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa cacaaagata
      301 caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca tccagaaatc
      361 caagttcaca accaatataa acaattatca acaatgttca attttatatc agacatgttt
      421 ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta aatgatgcgg
      481 ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat tacgcagaac
      541 gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt aaacatatcc
      601 gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag cagaagggaa
      661 aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac gagtaaagca
      721 actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa acgaaaaaag
      781 acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg aacccgtaag
      841 ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac ctcggcacgg
      901 atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct ggttgagttt
      961 gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg gttttgttgc
     1021 tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc agcgccgtca
     1081 tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa gcgcctgctc
     1141 cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga ccaacaaggc
     1201 caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac ctgttccttg
     1261 cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat ctttcccact
     1321 gaagcgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt ttatcgcatt
     1381 gtatcaactg cggaaaaaag cagcaaaacc gatgtgttcg ctgagaaaaa aggcgacaat
     1441 cgccgccttt ttagccagtc atttatcgtt ttaaacggct tttactgcat cggcgatgcg
     1501 gtgtgcaaat tcagtcacct gcgcttcgtc ttcgccttcc accatcacgc gaattaacgg
     1561 ttcggtgccg gatttacgca gcaacacgcg tccacggttg cccagcgcag cttcaacctc
     1621 tgcggtcacg gctttaactg actcatgctc aagcggatcg ccgctacctg cggtgtaacg
     1681 cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt gcaggctcat
     1741 atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt caccggtagt
     1801 ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc agcctttctc
     1861 ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg gaatacctaa
     1921 ctgtttcagc gccagctcaa gccccatgtt actcatcaat gtacccacag caccaccacg
     1981 cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat caccatcgac
     2041 tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga aggcaatacc
     2101 gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg tagcccccac
     2161 ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt tcgcccccag
     2221 ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat ccaccacaat
     2281 cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact cgatatagcg
     2341 acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa cacagctgat
     2401 ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt tggtgccgtc
     2461 gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag atatcacaat
     2521 tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg gcatcgggcc
     2581 agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg actccagcat
     2641 atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt ggcgcgccag
     2701 cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg gcgcatcccc
     2761 tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag cgtttgtttt
     2821 cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct ttgacgtcat
     2881 gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc agactaccgc
     2941 tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt cgtgacatac
     3001 ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt aatgaatagt
     3061 tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc tcttttgcga
     3121 tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact tctgcaaaga
     3181 catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata tgcatcagac
     3241 aaaccggtaa accggtttct gccgccgcct ccagagcgcc aggttcggaa agggagcgga
     3301 tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct ggtttggatg
     3361 tatctaccga gagccagact tcgaagcgtt gagcaattgc ctcaaccaca ggaataacac
     3421 gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac tcgccaccaa
     3481 cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc gcatctatca
     3541 gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg atccccatta
     3601 cgtgaggatg gctaaggtcc agtgaagtgc cttgggcaaa gagtttcatg gtgttatccc
     3661 tgctatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg tacaaataca
     3721 gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt tacccgggtt
     3781 cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt cgccagaatt
     3841 gttagaagtg cctggttctt cccagcccgc tggcggacgt acatcgcgac gtgccatcag
     3901 gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt tcatcgcatg
     3961 cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac gctcaatcag
     4021 tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt tcgctttcgc
     4081 tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg gacccaattt
     4141 ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa ctttaatatc
     4201 gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg ccagacgacc
     4261 accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga ttgcgtcgcc
     4321 ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg tcactttgtg
     4381 caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt cgtggtaagc
     4441 cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac ccatcatgat
     4501 tttatctttc gctttttcga actcaatcat cgacacaacg cgtttgttgc cacgagcagc
     4561 gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac taggagtacc
     4621 acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca tgtgaacttt
     4681 caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac ggtcgaaacg
     4741 gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg cgataacgat
     4801 gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca gagtctgttc
     4861 acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg cgtcgatttc
     4921 atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt cacgaacacg
     4981 ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag taaagaacgg
     5041 aactttcgct tcgcctgcaa tcgctttcgc cagcaacgtt ttaccggtac ccggaggacc
     5101 gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc tcggctcgcg
     5161 cagatactct accagttcag caacttcttc ttttgcttcg tcgcagcccg caacgtcagc
     5221 aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac caaacgacat
     5281 ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaga caccaatcag
     5341 caacagcatc gggaaccaag agatgaagat agaagccagc aggcttggtt cttcaggcgg
     5401 ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg gatcctgaac
     5461 cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt cacgtccgtt
     5521 gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag agtaatccac
     5581 cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca gcacaacggc
     5641 aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa cctcttatta
     5701 caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa actttcgtct
     5761 gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc gcgaacgtgc
     5821 acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa tttcccttag
     5881 atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac ctggcgctaa
     5941 tacatcacga cacatttcta acgccagttc caccagatac atggcacggg ggatatccac
     6001 cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga ctttgctgtc
     6061 gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc cctgaagaaa
     6121 gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc ggcctttgcc
     6181 gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa ggtcgacaac
     6241 cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt taaaccaggc
     6301 acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa agtgttcctg
     6361 aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt cccatgggga
     6421 taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata tatgggagat
     6481 ggcggtagaa tgacccgttt tcaatcccaa cgtaagcaaa aaatacgatg aatctgagta
     6541 ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt gttctgcttg
     6601 gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta gagcaccatg
     6661 aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg atcgtggaag
     6721 ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg ctggtgcttt
     6781 atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct aaagttacac
     6841 acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg ccacgctctg
     6901 ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca tcaacatctt
     6961 gatgtattgg gtaattctta caggtattcc accttaatta cttcaaattc tacttcgccg
     7021 cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc acgagcaata
     7081 ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc aacgatgcga
     7141 taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc aaaaataacg
     7201 cgcccattgt tgggcatttt ggtgacatca atcacctgcg cgttcgacag cttggcttcg
     7261 atgtctttaa tacggccttc gcagaaaccc tgctgttcac gagctgcgtg gtattcggcg
     7321 ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat ttcaggacgg
     7381 cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg taaggtcatc
     7441 ggaatagttt gcatttgtta tacctcttga atattcctga tggggcaagt cttcacccac
     7501 cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa aaaaataccg
     7561 acccgggtac aagtcctagg tcagctacaa ttcacatttt gatagtcatt ttaccctgaa
     7621 gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga cggctcgatt
     7681 ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt gaccagctgt
     7741 atagcgttca gtgttcaggc cgcgaatgtt gatgagtacg ttactcaact ccccgctggt
     7801 gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat tgattaccac
     7861 agtcagcaga tggcgctgcc cgccagtacg cagaaagtga ttactgcgct ggcggcgttg
     7921 attcaactcg gccccgattt tcgtttcacc acgacgcttg aaaccaaagg caatgtggaa
     7981 aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac gttaaaacgt
     8041 caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca aatcgatggc
     8101 aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg ctggccatgg
     8161 aatgacatga cacaatgctt tagcgctccg cctgccgctg ccatagttga ccgcaactgt
     8221 ttctccgtct cgctctacag tgccccaaaa cctggtgata tggcttttat acgcgtggca
     8281 tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgagg ttctgccgaa
     8341 gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac gctgacggga
     8401 tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga tggagccagc
     8461 tatgccgggg caattctgaa agatgagtta aaacaggcag gtatcacctg gagcggaaca
     8521 ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa acagtcggcc
     8581 ccgttgcacg atctgcttaa gattatgctg aaaaagtcgg acaacatgat cgccgatacg
     8641 gttttccgca tgataggcca tgcgcgcttc aatgtgcctg gaacatggcg ggccgggtcg
     8701 gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa caccattatt
     8761 gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat gatgcaggtg
     8821 ttgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct gccgctggcg
     8881 ggctatgatg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt ggatggaaaa
     8941 gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt cattaccaca
     9001 gcgagcgggc aacgaatggc gtttgtacaa tatctttctg gctatgcagt agaaccggcg
     9061 gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta taaagatatt
     9121 tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggttttg ctgattaacg
     9181 cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcatcat cccagtcctc
     9241 atcatcctct tcagcaatct cttcaagctg ctggcgatga taatcatccc acatgaattc
     9301 gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa tgataaaggt
     9361 catcacatcc cagcagagat ctttcacgcc cagtccactc gccgcagaga tcagataata
     9421 tttatcttcc cagcccagcg cttcagcaat cgctttcgct ttctcttcag cttctgcctt
     9481 atccagcaga tcgatcttat tgaacactaa ccaacgcggt ttcgccgcca gatcctggct
     9541 gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg tgccgtcaat
     9601 cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg
     9661 gatacccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac
     9721 gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag tggtaaacgg
     9781 ataatccgcc actttcggtt tagccgccga taccgcgcga ataaaggtcg atttacccgc
     9841 gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca gcagctcacg
     9901 cttatcaccc ggcgtaccgt tggttttctg ccgtggtgta cggttaacgg acgatttgaa
     9961 acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct gaccgtgttt
    10021 agtcatatcg cccatggttt caccagtacc ctggtcgatt acacgcgtac ctaccggcac
    10081 tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc
    10141 gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca ggttttcgtc
    10201 ggcttctatc catacgtcac caccatcacc accgtcgccg ccatccgggc cgcctttcgg
    10261 aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg caacgaccag
    10321 aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc ctgcgcgggg
    10381 ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac caatggcgga
    10441 atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt ttaacatcgg
    10501 tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca gcgtaaacag
    10561 tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc
    10621 cagggcgcca tatcctacca atgtattcag tccgcaaaaa attaaacatg cgagctgcca
    10681 gtggctaagc tgcgctatca ctcctggctt tgccagaggg aagagcgcaa ttgtacataa
    10741 actgtacagc aaaaacagga tctgcggtga ggccagccga cgcaataaaa ccttttgcgc
    10801 cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagatgactc cccaggtgta
    10861 atcagtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca ccaggccgct
    10921 caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca ggataaatac
    10981 gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag cggtcggact
    11041 caggtattgc aaggatgcgc tgaacaggat gaagttccca aacagcccgg cggtcgccac
    11101 tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca acctcttctt
    11161 caccgcaaga atggcaccca ggccaatact cgccatcaag aaacggtaaa acacgattgt
    11221 cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc cccagcaaat
    11281 tgctgtggtg agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac cgtattccct
    11341 gcaaagcgca tttgcggatt aacgtcgcac catgctcatt tttaccggtt atcgaatgta
    11401 aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa aaacttattc
    11461 agcttcgatg ctgataaatt tatggttttt cgggccttta acttcgaatt tcactttacc
    11521 gtctgcttta gcaaacagag tgtggtcacg accgcaaccc acgttagcgc cagcgtggaa
    11581 tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc caccgaaacg
    11641 cttaacgccc aggcgtttag cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt
    11701 tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt aattttcaca
    11761 tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg acgaaactta
    11821 acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat tacgccgcca
    11881 tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag cacttcagcg
    11941 aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg
    12001 cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa ctccgcttcc
    12061 gcgcacacct tttcaatgat tcagagtgcg ctataaatat tcacaatagg gcgcgaatat
    12121 tacgcaaaac gcacgccttt gacaagtgct acagtcaata tacgaagaaa aaaaacacaa
    12181 cttgtacggt aacgtttatc tgtgccattt tttcagtaca atcaccctat attcctaacc
    12241 ataaacccta agttgctttt gttcacagta aggtaatcgg ggcgagaagc ccggcttttg
    12301 cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg
    12361 caatccttga gcagcttaat tccgacgtcc aactgatcaa tcagttaggc tattacatcg
    12421 tcagcggcgg cggtaaacgt attcgtccga tgattgctgt actggctgca cgagctgttg
    12481 gctatgaggg aaatgcgcat gtcaccattg ctgccctgat cgagtttatc cacacggcga
    12541 ctctgctaca cgacgacgtt gtggatgaat cagatatgcg caggggtaaa gctaccgcca
    12601 acgctgcatt tggcaatgcc gccagcgtgc tggtaggcga ttttatttat acccgcgcgt
    12661 tccagatgat gaccagcctc ggttcactca aagtgctgga agtcatgtca gaagctgtaa
    12721 acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt taacgatccg gacatcactg
    12781 aagaaaacta catgcgcgtt atctatagca aaaccgcgcg tctgtttgag gctgccgcgc
    12841 agtgttccgg gattctggct ggctgtacgc cggaggagga gaaaggcctg caggattatg
    12901 ggcgctatct cggcactgct ttccagttga tcgacgattt actcgattac aatgccgatg
    12961 gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga aggtaaaccg acgctgccgc
    13021 tgctgcatgc gatgcatcat ggcacaccag aacaggcaca gatgatccgt accgccatcg
    13081 aacagggtaa cggtcgccat cttctggaac cggttctgga agcaatgaac gcttgtggat
    13141 ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga caaagccatc gcagcgttac
    13201 aggtgctccc ggacacccct tggcgagaag cactcatcgg cctcgcgcac atcgctgttc
    13261 aacgcgatcg ttaatcccct cccttcatcc cgcgcagtgc gtgggatgat tccagtaaat
    13321 tccataaaaa cactaattca gcgctataca cctgaaaaaa agggcgatga atctttatat
    13381 attctgaaca ttcacactct ttacaggaac tttttagagc aataggccat caggagtata
    13441 gtgatgcccg acagaagaag tgttctgaat gaaagcgact aacttaagga gtgaggaaag
    13501 atggaaagta atttcattga ctggcatccc cctgacatca ttgcaggttt gcgcaagaaa
    13561 ggaacgtcaa tggcggcgga atctcgcaga aatggtttga gttcctcaac gctggcaaac
    13621 gcattatcgc gcccatggcc gaaaggagag atgattattg cgaaagccct gggaactgac
    13681 ccctgggtta tctggccatc acgctaccat gatccgcaga cccatgagtt tatcgacaga
    13741 acgcagttga tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg gtagccccgc
    13801 gaacggggct gccagctctc agacgattac tcgcctttca cacgcttaat atttgcacct
    13861 aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat cgatgtgata aatacgatca
    13921 accaccgtcg tcccttccgc aatacagcca gccagcacca ggcttgctga tgcacgcaga
    13981 tcggttgcca taacctgtgc accagaaagt ttttcaacac catgacaaat aacggtattg
    14041 ctttcgattt cggcatgcgc gcccatacgg ctaagctccg gcacatgcat aaagcggttt
    14101 tcaaagaccg tttcggtgat gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac
    14161 tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc
    14221 ggacgtttgc catgcatatc caggctaatc cagtcttcgc cgacttcgat gtccgctcca
    14281 gcgtcacgca gtttcgccag cacggcgtcg agagtatctg gctgcgcgtt acggcagata
    14341 attttgccgc gagaaatcgc cgccgccacc aggaaagtac cggtttcgat acgatccggt
    14401 agcacgcgat agacaccgcc gcctaaacgt tccacacctt cgatgacgat acgatcggtg
    14461 ccctgaccgc taattttcgc acccagcgta atcaggaagt tcgcggtatc gacgatttcc
    14521 ggttcacgcg ctgcgttttc aataatcgtg gtaccttccg ccagggttgc agcacacatg
    14581 atggtcaccg ttgcgccaac gctgacttta tccatcacga tatgtgcgcc tttcagacga
    14641 ccatcgacgg aagctttaac gtaaccttct tccagtttga tggtcgcgcc taattgttcg
    14701 aggccagaaa tgtgtagatc aaccggacgc gcaccgatcg tacaaccgcc aggcagtgaa
    14761 acttgcccct gaccaaagcg cgctaccagc ggccccagtg cccagataga agcacgcatg
    14821 gttttaacca gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc aatatgcaca
    14881 gaaccattac gttctacttt cgcacccagc tgacttagca gcttcattga tgtatcgacg
    14941 tctttcagtt tcgggacgtt ctggatctct accggttctt ccgccagtag agcggcaaaa
    15001 aggataggca gagcagcatt tttagcgccg gaaattgtga cttcgccctg gagcttcgtt
    15061 ggcccctgaa cacgaaattt atccatttag tttgttctca gttaacaatt catatccgct
    15121 accggcgaat cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca
    15181 ggggtatacg ctttgatcga cacagcatga atgcggttat ccgcaatata ttccatcagc
    15241 gggccataga ccgtctgctg ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg
    15301 gcaataacct gaaagtggct gccatcgccg gaaacgtgga cttcctggag ggagagagcg
    15361 ttcatcaaca cgctctgaat ttcattattt tccatgggat cttcaatcat cagttaataa
    15421 accagcgaaa catcttagag caaagttgcg ctggcataaa taagcaaaaa gcctcgctga
    15481 taaatcagac aaggctcgac ttgcaggcag gtttgccgga caggcggtta acgccatatc
    15541 cggcctgaaa aaatttaacg aggcagaaca tcagcaggca aattatacaa tttcgccagg
    15601 gtatacactt tgtcgtttac cccctgaagc gtcacattgt tgccctgctt tttcgccaga
    15661 tcgataagat ggagcagcag tgccagtccc cccgtatcca cgcgggacac acggctaaga
    15721 tcgatgcagg taatcccctt caccgcttcc tcacgcattt cccaaagcgg tagcaaaacg
    15781 tcctgatcca gctctccgga taacgccagc gtgtcacccg tctgcatcca gctcagtgac
    15841 tcgctcatta ttttttctct tccagagtga ttttctgttg agaaatcgat ttcagttgcg
    15901 cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg
    15961 tgatcatact gacgccttca gcaatcatgt cgtaagcctg ccagttgccc gtctgggagt
    16021 ttttacgcca ctggaagtcc agacgcaccg gcgggcggcc attcggatca ataatggtaa
    16081 cgcgaatcgg cacaatggtt ttatcgccca gcggctgttc tggcgcaatc tgataggttt
    16141 gaccgtgata catcgccagc gcctgaccgt aagcctgctt caggtactca cggaaagcgg
    16201 caaagtaggc ttcacgttga gcaggggtcg cactcttgta atactggccc agcaccagcg
    16261 caccggcgta tttcacctgt acgtatggca gcagttcctg atcaacaatg gtacgcagat
    16321 aatccgggtt ggcccgaatt tgcggttgct cattcttcag gcgatcgaac gttttctgcg
    16381 ccgcctcgtc catcagctta taaggattgg tctggtctgc cgcggttgcc gcactcagcg
    16441 gtgcaatcac cagcaaagcg accatcatta aacgtttaaa catgcgtcgg ttctcctgaa
    16501 attatttcgt tgtacccaca ggttcagtgg tttcattatt acctggcgca gcagctggcg
    16561 catcgccact attcttattg tcatcgcctt tactaccgta aaggaactga ccaatgagat
    16621 cttccagcac catcgcagac ttagtgtcct gaattgtatc gccatccttc aggatagcag
    16681 tccccagttc cgggtcttca aaaccgacgt ttaatgccag atattgttcc cccagcaggc
    16741 cggaagtacg aatgctcagc gaactggtat ctggaatgtg gttataacgt tgttcaattt
    16801 ccagcgttac gcgtggcaga taggttttcg ggtccagcgt aatatccgcc acccggccca
    16861 caacaacgcc accaatactg accggagagc gcgctttcag gccgccaatg ttatcgaacg
    16921 tcgcataaag cgtgtaggtc ggttcagtac gtatggacgt cacgttcgcc gccttcaggc
    16981 aaacaaacag agccgccagc agtgctgcta ataaaaagat acccacccaa atttcatttt
    17041 ttttcgtttg catgaactca attcccaaac atcaatgcgg tcagcacaaa atccagcccc
    17101 agaacagcca gagacgagtg gacaacggtg cgagtggttg cccggctaat cccggcagac
    17161 gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc
    17221 acgctcttaa tcagacagtt gaccagatcc atacgccagt cgacggcatt ttgcattgcc
    17281 gaccagaaga acccgctatc aatgcctttc caactgacgc cgaccagaga tccgccccag
    17341 atccccacgg cgacgaaaat aaccgtcaac agtggtaatg aaataacccc agcccagaaa
    17401 cggggagaaa taacccgacg cagcggatcc accgccatca tctccatact ggagagttgc
    17461 tctgtagcgc gcatcaggcc gatttctgcg gttagcgccg aaccagcacg cccggcaaac
    17521 aacaacgcgg caaccaccgg ccccagttca cgcagtagcg ataacgccac cagcataccc
    17581 agactggttt ccgcactata agtggtcaga accagataac cttgcagccc caacaccatt
    17641 ccgatgaaca cgccagaaac cacaataatc agcatcgaca ggacgccgac attatagagc
    17701 tggcgcacca gcagcggcgc atgtttgcga aattccggtt tgccgaccag cgcattgaat
    17761 aacattaacc cggcccgccc gaacgttctc agggttttaa tccctttatg tccgagcgac
    17821 gccagcgcat ttaacagcat gagtggctta actccctggt aaaagatcag cgtgataatc
    17881 gccggcagga tagcggaacg gaacaggccc gtcagctatc ccgtccagaa actgacgtac
    17941 gcgcggatca ggattcgcct gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc
    18001 cgccaggatc caggcgtgat ccgcaatact taacacttcc ggcacatcgt gagaaaccac
    18061 cacacaagtc acgcccagcg cgctgttcag ctcagaaatc agcttcacca gtacgcccat
    18121 ggtgatggga tcttgcccaa caaaaggttc atcaaacatg atgagatccg gctccagcgc
    18181 aatcgcacgc gccagcgctg cacgccgcgc catcccaccg gaaagttcag aaggcattag
    18241 tttagccgct ccacgcagcc ccacggcctc cagcttcatc atcaccgtac tatgcaacaa
    18301 tggcgcggga agttgggtat gttcgcgcag tggataggcg acgttgtcaa atacgttcat
    18361 atcagtgaac aacgccccgg actgaaataa catgctcatc cgtttgcgca ctgtatacag
    18421 gcgcgaacga gacatcgccg gaatattctc accatcgaaa aggatctcac catgatctgg
    18481 tgcgatttgc ccgccaatca gacggagtag cgtcgtttta ccgatgcccg atggccccat
    18541 gatcgccgtg atcttccctc gcggcacggt cagggaaata ttatcgaaga tgcagcgatt
    18601 gccacgcgta aaactgacat cgcgcatatc gactaaattc gccacagact gctccataat
    18661 tcacccttcg tcttgcgttg attttctaag catggcgctc aatttaacct tgaacccaac
    18721 atatttacag aatattaccc gccgtggtta gcgaaagctg gcatttgttt tactttttag
    18781 ccgcataaag tcaaaattaa gaatccgtta cggctttctg aaaatcttca gcggaccggc
    18841 gagtatacct gaagaaagga cgttagatgc ttttagctac ggcactgtta attgttggtt
    18901 tacttttggt cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa
    18961 cctttggcat cccgccgctg atcatcggca tgacggtggt cagtattggt acatcgttac
    19021 cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta gccgttggta
    19081 cagccctcgg ctcaaacatt atcaatattt tgctgatcct cggtctggct gcgctggttc
    19141 gtccttttac cgtccattcc gatgttctgc gtcgtgaatt acccttaatg ttgttggtca
    19201 gcgtagtggc cggttccgta ctctatgacg gacaacttag tcgcagcgat ggtatctttc
    19261 tcctctttct ggctgtgcta tggctgctgt tcattgttaa atttgcacgt caggctgaac
    19321 gtcaggggac tgacagcctg accagagagc agcttgcaga gctgccgcgt gacggcggat
    19381 tgcccgtcgc gtttttatgg ctcggcattg cgcttatcat catgccagtg gccacgcgga
    19441 tggtggttga taacgccacg gtgctggcga attactttgc catcagcgag ttgacgatgg
    19501 gtctgacggc aattgctatc ggaaccagcc tgccggaact ggcaaccgca atagcggggg
    19561 ttcgcaaagg tgaaaacgac attgctgtcg gaaatatcat tggcgcaaac atttttaata
    19621 ttgtcatcgt gttgggttta cccgcgctga taacgccagg agagattgat ccattggcgt
    19681 acagtcgtga ctacagcgtg atgttgctgg tgagcattat ttttgcgttg ctgtgctggc
    19741 ggcgctcccc gcaaccgggc cgtggtgtag gggtattatt aactggcgga tttatcgtat
    19801 ggctggcgat gttgtactgg ttatcgccaa tactcgttga ataactggaa acgcattatg
    19861 tcgcacgtag agttacaacc gggttttgac tttcagcaag caggtaaaga agtcctggcg
    19921 attgaacgtg aatgcctggc ggagcttgat caatacatca atcagaattt cacgcttgcc
    19981 tgtgaaaaga tgttctggtg taaagggaaa gttgtcgtca tggggatggg gaaatcgggg
    20041 cacatcgggc gcaaaatggc cgcaacgttt gccagcaccg gtacaccttc atttttcgtc
    20101 catccaggtg aagccgcgca tggtgattta ggcatggtca ccccacagga tgtggtgatt
    20161 gctatctcta actcaggtga atccagcgaa atcacggcct taattccagt gcttaagcgt
    20221 cttcacgtac cgttaatctg catcaccggt cgcccggaga gcagcatggc gcgcgccgca
    20281 gatgtgcatc tgtgtgttaa agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc
    20341 agcaccacca ccgccacgct ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca
    20401 cgcggcttta ctgctgaaga ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa
    20461 cttctgctgc gcgtaaacga tattatgcat acgggcgatg agatcccgca tgttaagaaa
    20521 acggccagtc tgcgtgacgc attgctggaa gttacccgca aaaatcttgg tatgactgtc
    20581 atttgcgatg acaatatgat gattgaaggc atctttaccg acggtgattt acgccgtgtc
    20641 ttcgatatgg gcgtggatgt tcgtcagtta agtattgccg atgtgatgac gccgggggga
    20701 atacgcgtgc gccctggcat tctggccgtt gaggcactga acttaatgca gtcccgccat
    20761 atcacctccg tgatggttgc cgatggcgac catttactcg gtgtgttaca tatgcatgat
    20821 ttactgcgtg caggcgtagt gtaaagattc aaggataaac aacaatgagc aaagcaggtg
    20881 cgtcgcttgc gacctgttac ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca
    20941 ttcgtctgct gatcctcgac gttgatggcg tactgtcaga tggcctgatt tatatgggca
    21001 ataacggcga agagctgaaa gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc
    21061 tcacctctga tattgaagtc gctatcatta ccgggcgaaa ggctaaactg gtagaagatc
    21121 gttgtgccac attggggatc actcacttgt atcaggggca gtcaaacaaa ctgatcgcct
    21181 ttagcgatct gctggaaaaa ctggcgattg ccccggaaaa tgtggcttat gtaggcgatg
    21241 atctcatcga ctggccggta atggaaaaag tgggtttaag cgtcgctgtg gccgatgctc
    21301 atccactgtt gatcccgcgc gccgattatg tgacgcgcat tgctggcggt cgtggcgcag
    21361 tgcgagaagt ttgcgactta ttactcctgg cgcagggcaa actggatgaa gccaaagggc
    21421 aatcgatatg agtaaagcca gacgttgggt tatcattgtg ctatcactgg cggttctggt
    21481 gatgatcggc attaatatgg ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa
    21541 tgatcccacc tataaaagcg agcatacgga cacgctcgtc tataacccag aaggggcact
    21601 aagctatcga ttgattgctc aacacgttga atattattcc gatcaggccg tttcgtggtt
    21661 tacgcagccg gtacttacca cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc
    21721 agataaagcc aagctgacca atgaccggat gctctattta tatggacacg ttgaagtcaa
    21781 cgcacttgtg ccggactctc aacttcgcag aatcacgacg gataacgcgc agatcaatct
    21841 ggtgacgcag gatgttacct ctgaagacct cgtcacgtta tacggaacaa catttaactc
    21901 cagcggtctg aaaatgcgcg gcaacttacg cagcaagaac gccgagctga ttgaaaaggt
    21961 tagaacatcc tatgaaattc aaaacaaaca aactcagcct taatcttgtg cttgccagct
    22021 cacttctggc cgccagcatt ccggcatttg ccgtaaccgg agacactgat cagccgatcc
    22081 acattgaatc ggaccagcaa tctcttgata tgcaaggcaa cgtggttacc tttaccggta
    22141 atgtcatcgt cacccagggc accatcaaaa ttaatgccga caaagtggtc gttacccgtc
    22201 cgggcggcga acaaggtaaa gaagtgattg acggctacgg taaaccggca acgttctacc
    22261 agatgcagga caacggtaaa cccgttgaag gtcatgcttc ccagatgcac tacgaactgg
    22321 caaaagattt tgtcgttctg acgggtaatg cttatctgca gcaggtcgat agcaacatta
    22381 agggcgataa gatcacttac ctggtgaaag agcagaaaat gcaggctttc agcgacaaag
    22441 gcaagcgcgt aacaaccgtt ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc
    22501 agaccccggc acagaagaag ggtaattaat tcgttatggc aacattaact gcaaagaacc
    22561 ttgcaaaagc ctataaaggc cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg
    22621 gggaaattgt cggtctgctg gggccaaacg gtgccggtaa gaccaccact ttctatatgg
    22681 ttgttggcat tgtgccgcgc gatgcgggca acatcattat tgatgatgac gatatcagtc
    22741 tgctgcctct gcatgcacgc gcgcgccgcg gtatcggcta tctgccacag gaagcctcca
    22801 ttttccgtca cctcagcgtt tacgataacc tgatggcggt actgcaaatt cgtgacgact
    22861 tgtctgctga acaacgtgaa gaccgcgcga acgagctgat ggaagagttt cacattgagc
    22921 acctgcgtga cagcatgggg cagtcactct ccgggggtga acgtcgccgt gtagaaattg
    22981 cccgcgcact ggctgcgaat ccgaaattta ttctactcga cgaaccgttt gccggggttg
    23041 acccgatctc ggttatcgac attaaacgca tcattgaaca cctgcgcgac agcggcctgg
    23101 gcgtactgat cactgaccac aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata
    23161 tcgtcagtca ggggcatttg attgcccacg gcacgcctac agaaatctta caagacgaac
    23221 acgttaagcg tgtatacctt ggggaagact tcagactctg atagggtaga agtttgcgac
    23281 gttttagcag gagagtacga ttctgaacat gaagcaaggt ttgcaactca ggcttagcca
    23341 acaactggcg atgacgccac agcttcaaca ggcgattcgt ctgttgcagt tgtcgacgct
    23401 ggaacttcag caggagctac agcaggcgct ggagagtaat ccgctgcttg agcaaatcga
    23461 cactcatgaa gaaatcgaca cccgcgaaac gcaagacagt gaaacgctgg acaccgccga
    23521 cgcgctcgaa caaaaagaga tgccggaaga gctgccgctc gatgccagtt gggacaccat
    23581 ttacaccgct ggtacaccat ccggcaccag cggtgactac attgacgatg agctgccggt
    23641 ctatcagggc gaaacgacgc agaccttgca ggattacctg atgtggcagg tcgagctgac
    23701 accgttttcc gacactgacc gcgctattgc tacctctatc gtcgatgccg ttgatgacac
    23761 cggttatctg actgtcccgc tggaagatat tctcgaaagt atgggcgatg aagagatcga
    23821 catcgacgag gttgaagccg tccttaagcg gatccaacgg tttgatccgg tcggtgtagc
    23881 ggcaaaagat ctgcgtgact gcctgctaat ccaactctcc caattcgata aaaccacgcc
    23941 gtggctggaa gaggccagac tgatcattag cgatcatctc gatctgttag ccaatcacga
    24001 cttccgcact ttaatgcgcg tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa
    24061 tctgatccag tcgctcgatc cgcgccccgg gcagtcgatc cagactggcg aacctgagta
    24121 tgtcattcca gatgtgctgg tgcgtaagca taacggtcac tggacggtag aactcaacag
    24181 tgacagcatt ccgcgtctgc aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg
    24241 caacgatggt gacagccagt ttatccgcag caatctgcag gatgccaaat ggttgatcaa
    24301 gagtctggaa agccgtaacg atacgctact gcgcgtgagt cgctgtatcg ttgaacagca
    24361 gcaagccttc tttgagcaag gtgaagaata tatgaaaccg atggtactgg ccgatatcgc
    24421 ccaggctgtc gaaatgcatg aatcgacgat atctcgcgtg accacgcaaa aatacctgca
    24481 tagtccacga ggcatttttg aactgaagta tttcttttcc agtcacgtca ataccgaggg
    24541 cggcggcgaa gcttcctcca cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga
    24601 aaacccagcg aaaccgttga gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat
    24661 catggtggca cgccgcactg ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa
    24721 ccagcgtaaa cagctcgttt gacccaaccg ataaggaaga cactatgcag ctcaacatta
    24781 ccggaaataa cgtcgagatc accgaggcac tgcgcgaatt tgttagagcc aaatttgcca
    24841 aacttgagca atattttgac cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag
    24901 tcacccacac ctcagatgca acactgcatg taaacggtgg tgaaattcat gccagcgcgg
    24961 aaggtcagga catgtacgct gccattgatg gcttaattga taagctggca cgccagttga
    25021 ccaaacataa agataaactg aaacaacact aattgtccgg gcaattagca tgtgcatggc
    25081 ggcctgttgt gcggcacaac gggccatttg tacggttaat gctccgagcc tgttccactg
    25141 tttgagtggg caggttctta ggtgaaatta tgacaaataa tgatacaact ctacagctta
    25201 gcagtgttct taacagggaa tgtacgcgaa gccgcgttca ctgtcagagc aaaaaacgcg
    25261 ccctggaaat catcagcgag ctggcggcga aacaacttag cctgccgcct caggtggttt
    25321 ttgaagctat cctgacgcgg gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca
    25381 ttccgcatgg caaactggaa gaagatactc tgcgcgccgt tggcgttttc gttcagctcg
    25441 aaacgcctat agctttcgat gccatcgaca accaaccggt ggacttactt tttgccctgc
    25501 tggtgccggc agaccaaact aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc
    25561 tggcggacaa aaccatctgc cgccgtttac gcgcagccca gagcgatgaa gagctgtatc
    25621 aaatcattac ggataccgaa ggtactccgg atgaagcgta gttattcggt aatgtctctt
    25681 ttagacgttg tgaggagaaa cagtacatgg tactgatgat cgtcagcgga cgttcaggtt
    25741 caggtaaatc tgtcgccctg cgtgcgctgg aagatatggg tttttactgc gtggataacc
    25801 ttcccgtagt gttgttaccc gatctggctc gaaccctggc cgatcgcgag atttctgccg
    25861 ccgtcagcat tgatgttcgt aatatgccgg agtcaccaga aatattcgaa caggcgatga
    25921 gtaacctgcc tgacgctttc tcaccgcaac tcctgttcct ggatgccgac cgtaatacct
    25981 taattcgtcg ttacagtgac acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc
    26041 tggaaagtgc tatcgacaaa gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc
    26101 tgattgtcga tacctcagaa atgtccgttc acgagctggc tgaaatgctg cgtacccgtc
    26161 tgctgggtaa acgtgaacgc gaactgacca tggtctttga gtctttcggc ttcaaacacg
    26221 gtatccctat cgatgcagat tacgtctttg acgtgcgctt cttgccgaac ccgcactggg
    26281 atccgaaact gcgtccaatg acaggtcttg ataaacctgt cgccgcgttc ctcgaccgcc
    26341 acacagaagt acacaatttt atctaccaga cgcgaagcta tcttgagcta tggttaccta
    26401 tgctggaaac caacaaccgt agctacttga cggtcgccat tggttgtacc ggcgggaagc
    26461 accgttcggt gtatattgca gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg
    26521 tccagtcacg ccatcgtacg ctggaaaaac gtaaaccatg accgtcaagc aaactgttga
    26581 aatcacaaac aagctgggca tgcatgcccg gcctgcaatg aagctgtttg aattaatgca
    26641 gggttttgac gctgaagtgc tgttacgtaa tgacgaaggc accgaggccg aagccaacag
    26701 cgttattgcg ctgctgatgt tggattctgc caaaggacgg cagattgaag ttgaagcgac
    26761 cggcccacag gaagaggaag cactggccgc cgttatcgcc ctctttaact ctggttttga
    26821 tgaagattaa tcttcatcac tttgacatac aactcccttc aaactccccc cgacaataag
    26881 aaaatcacgt actgaaatcg ttctcaatca acgtcatttg tacattttgt gcgcttttca
    26941 ctttcagaag aaccttaaga aaaccttaag aggcattgtt taggttttgt ttaagttaat
    27001 cgaccatact ggagatcgtc agaaaatatt tccaggagat ggcatgattc gcttatctga
    27061 acaaagtccc ctgggcaccg gaagacatcg gaaatgttat gcgcatccgg aagatgccca
    27121 acgctgtatc aagattgtct accatcgtgg cgatggtggc gataaagaga tccgccgtga
    27181 gttaaagtac tacgcgcatc ttggtcgccg gttaaaagac tggagtggaa taccgcgcta
    27241 tcacggtacc gttgagacgg attgcggaac cggttatgtc tacgatgtga ttgcagattt
    27301 tgacggcaaa ccctccatta cgctgaccga atttgctgaa caatgccgtt acgaagaaga
    27361 cattgcacaa ctgcgccagc tactgaaaca gctgaagcgt tatttgcagg ataaccgtat
    27421 cgtgacgatg tcgctgaagc cgcagaatat cctctgtcac cacatcagtg agtcagaggt
    27481 tatcccggtc gtctgcgaca acattggtga aagcacattg attccactgg cgacctggtc
    27541 gaagtggtgt tgtctgcgta agcaagagcg gttatggaaa cgatttatcg cccaaccggc
    27601 gctggctatt gccctgcaga aagatctgca acctcgtgag agcaaaacgc tggccctcac
    27661 ttcgcgcgaa gcttaatcca gctggtgttg ttgcataaac ggctcaccgc ctaactgata
    27721 catctgccgt aaaatccacg cctgacggct acgcacgtag cccgatggcg aggagacttt
    27781 gaaacgaagt ggattaggta atacagctgc cagtaatgca gcttccgacc gggtaagttt
    27841 gctcgcgggt ttgtggaaat aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc
    27901 aaattcggcg atattcaggt aaaccgtcag gatacgcttt ttgctccaga ctgtttctat
    27961 ccccagcgtt aatccagctt ccagcccttt tcgtacccag ctacgcccat cccataaaaa
    28021 gagatttttg gccgtctgct gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg
    28081 ctcgttgtgc gccagggctt tctcaatgga agcgacatca aagccccagt gctcaggaaa
    28141 tttctgatct tctgcggcaa tcaccgccag tcccatccac ggcgagattt gatccatact
    28201 gacccagtca gaatgtgcca cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg
    28261 ctcgaccatt accgctgaga agggaacagg cgcaacacta aacaacgcga tgcccccgcc
    28321 ccagaaaacg gcgaggacaa ccattaaccg caaaagaaaa cggcgaacga aactaaacac
    28381 cgttaagcgg cttttactca ttcagccaga accagcacgc gggaaaccag cttatcaatg
    28441 ccgctcgccg cttctgcaat gttctgcgcc agcatatatg ctggggtggt gacaatctta
    28501 ttgtcttcat caaccacgat atcatcgaca ggacagggca catgctccgc gcccatctct
    28561 tccagcactt ctgcggtatc gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa
    28621 attttcggca gcatcgccgg ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc
    28681 atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc attcgctacc aagagtggca
    28741 aaattgctta aattcttcgc cgcgccaaac ccccccggca caatcaacgc atccagttca
    28801 gtggcatcgg cctgggccag aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc
    28861 agcacgttgc gcgtttccgt catcgcttcg ccagttaaat ggttgataac atcaacctgc
    28921 tgcttatccg gtgcaaagca gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc
    28981 aacaccgctt catgaatttc agaaccgtca tagacgccgc atccgctcag aattacgcca
    29041 attttcttca ttgtgatcat cctttcgcaa ttgactgaaa ctcattaatt tttttaataa
    29101 aaatggtacg catcacacat ttaactgatt catgtaacaa atcatttaag ttttgctatc
    29161 ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt aactcatcat aataatttac
    29221 ggcgcagcca agatttccct ggtgttggcg cagtattcgc gcaccccggt ctagccgggg
    29281 tcatttttta gtggcttttg ccacccacgc tttcagcact tctacgtcgt gacgccactc
    29341 ttctttcatc tcttcaatcc attcaccgac gttatcttcc caggcaggaa ggtcaggaga
    29401 ctgaatttgc tgacccagct gttgcagatg gcgtaacccc actgaccccg ccgcaccttt
    29461 aattttatgt ccttcctcaa caatgccttt tttatcctgc gccgtcagat tcgactccag
    29521 cacgctgaca tagcccggca tcatcttctc aaacaccgct aacccgtcgg tgatcagctt
    29581 cggtcctaca agttcgagat actgttccag catgagaata tcgagcaatg cttctgattt
    29641 actgttctct tctgtcgtca ccgtactctc ctcatcatcc tgggtatccc agaatttctt
    29701 gatcatcgcg gttagcgccg gaaccgaaag cggcttactc agcacatcat ccattccagc
    29761 attgaggtac tcttgtttgt ctttcagcac gttagcggtt aaggccacca gcggcggtaa
    29821 atcctcgcgc ggataacgct tcgtcagttc acgagagata tccagcccgg tcatatctgg
    29881 caactgaata tccagcaaca ccaggtcgta ttcgcccggt ttaaacatct ccagcgccgc
    29941 cttgccggtc atggcgacat caacgctgtt acctaatttt tccagcacag aacgcgcaac
    30001 aatcacgttc agttcaatgt cttccaccag cagcacattc agcgccggta aaggcatatc
    30061 gtcttcatca aacgcatcat cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt
    30121 aaaggttgaa cctttgccct gttcgctggt aaccgtaata tcgccgccca tatttttcgc
    30181 cagacgacga gaaacggcca gaccaatacc ggtgccggtg gcaggtttac cgccatgact
    30241 gtctttcacc tggtaataca tggcgaaaat tttatccagc tcatcctgcg gaatgccgat
    30301 accagagtct tccacttcaa aatgcagcat atcgccttca tcgtagcgca cgcgcacggt
    30361 aacctggcct tgctgggtga atttgacggc gttactgatg aggttccaca ggatctgccg
    30421 taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc agcgtcggct ccaggttaaa
    30481 gcgcaatcct ttttgttgcg cctgcaatgc ggagagattt tccagatcgg caaggaagct
    30541 ggtgaaatca accggttgat tatcaagctg gaccttgcgc cgttccatct tatccatgtc
    30601 gataatatcg ttaaagatat tccccagcgt gacggccgaa acatggatgg tcttgagata
    30661 tttttcctgc tcggcggtga gttcggtatc cagcagaatg cggctcagac cgacgatacc
    30721 gttcagcggt gtacgcaatt cgtgactgat ggtggagata aacgtcgttt tgtcgcggct
    30781 ggcccgttca agcgcatcct gataccgctt acgctcggta atgtcgcgac caaagcccat
    30841 caaaccgtga cgtttaccca cgcggtcgta gtacggcact ttacggattt caaagcaggc
    30901 tttgcgcccg tccgggtaat ccagccactg ttcataggtc agtgacacat tatgacggaa
    30961 cactttttca tcggtttcaa tgacttttgc ggcggcttcc ggtgagtaaa catcagcagg
    31021 tttcaggtga accagttgtt tttcgctttt tccggtcagc agctccatcg cgcggttaca
    31081 gccggaaaac tctttatctt cgttacgata aaaaaccagg tcgggtgaag catcaaggaa
    31141 ggaacgtaag aatgaggatt gctgctcgag ctgaatttgt gtctcttcgc gctctttgat
    31201 ttcaattttc agttggccga aggtttcctg cagttctgct tccgcttttt cacgaacggc
    31261 aatttcctga tttagctggg caatattatc ttttaactga acgttgaggc tcaaatcgcg
    31321 ctcgcgcatc tcctccagtt tttgcaccag ccgtgacaga cgttgtcgtg actcctccag
    31381 ttgctcgacg accaccgata gaaagtagac cgcccacggc gtaatcagca aaccaaagaa
    31441 gatagaacga ataacatcaa tgctttcgac ctgaccatgc agcaccatgg ttaccgccat
    31501 ttgtaccaca atggcaagaa cgacgagggc cagcgccagc aacattgaga agcgcaccag
    31561 acctaacttc atcatcaggt caacataata ctgcgccagc agacgaattt gcttcattag
    31621 ggaattcctt cacgacaacc tgccccaata atacccaatt ctgacagtta cgttataaat
    31681 tgtgtgagaa atgcggaatt cccctcaacg acctactccg ttggaggtag ccacggacgt
    31741 cccagcgccg atccctgtac gccatgttca ttcagatagc gatccagctc gaccattccc
    31801 gtccatcgat tttcgcacca cagcggagca agcagcgtcg ggcgacgggc gctggcggaa
    31861 atgcggtggt agatcacttc cggtggcgta tggcgaatca tttctccggc agtgagcgtg
    31921 taatcctcca gttcaatacc gttcaaacgc cccgcttccc aggctttcgc cataatgctg
    31981 cctttcacaa tatgcagcgg atgcagcttt atgccgtcca cgccggtttc aaccactcgc
    32041 tccagcgttt gcaaacattc agcctgccct tcgccaggca ggccgacaat taagtgggaa
    32101 cacaccttca gcccgcgctg acgtgccagc tgggttgtac gctgataaca ggcaaaatca
    32161 tggccgcggt tgatgcgatg cagtgttttg tcgtgggcgg tttgtagccc cagctccagc
    32221 cacacttcgt aaccctggtc cttatattcg caaagcagat ccagcaccgc atccggcacg
    32281 cagtccgggc gggtaccaac acacaaaccg acaatattgg cctggctcac cgcctgctga
    32341 tacatagaac gcagcacctg aacttccgca aaggtgctag tatacgcctg aaagtaggcc
    32401 agatagcgtt tagcgcggtt aactaaattc gcctggtgct ccagttgctc ggcaatggaa
    32461 cgatgctgct gcgcttcatc ggcaaacgag gcaacattac agaatgtgca gccaccacgc
    32521 ccgatggtac cgtcacggtt agggcagcta aaaccgccgt gcagcgtcag cttatgcacc
    32581 ttttgcccat aacgacgggt gagatcacca ccaaacatat tgactaattt ctgtaactgc
    32641 ataatctgat agaccgcgcc ttgaaaagag gccaaagcct gccattttta gctcaattcg
    32701 gcgatgacct ggatcaatcg tcccggcctg cttttatcaa ctgcataatc aatcaaaatt
    32761 accgaaattt catgcataat cacataaatc acttttgctt atcttgtgtc agattttttt
    32821 atctccttat ggattttagg caaaaacagt agcatgaaac gtcattacca attaaggcag
    32881 tataaaatgc tggttttctc gtcagttcaa ggcaggataa gggttaacac gcctttatga
    32941 cagtcaggaa ttgactgttt ctctaacgac ttccctttta gccttaaaga taaaattcat
    33001 ttaatttcag ttagttaata aagaattttg cgctaaagca catttctgta ccaataagct
    33061 tgccatttga cctgtatcag ctttcccgat aagttggaaa tccgctggaa gctttctgga
    33121 tgagcagcct gctcatcata tttatgcagt aattgagatc ccctcttcac cgtattaacc
    33181 gatgcgaaaa ggacaacaag ggggcgaatg cgaggcgcgc gtatgacacg caacccccgt
    33241 cgccacgctc tttctgtgcc cgtgcgcagc ggttcggaag tggggttccc gcagagcctg
    33301 ggggaggttc acgatatgtt gtacgataaa tcccttgaga gggataactg tggtttcggc
    33361 ctgatcgccc acatagaagg cgaacctagc cacaaggtag tgcgtactgc aatacacgca
    33421 ctggcccgca tgcagcaccg tggcgcgatt ctcgccgatg gtaaaaccgg tgacggttgc
    33481 ggcttgctgt tacaaaaacc ggatcgcttt tttcgcatcg ttgcgcagga gcgcggctgg
    33541 cgtttagcaa aaaactacgc tgtcgggatg ctcttcctga ataaagatcc tgaactcgcg
    33601 gctgccgcac gccgcatcgt tgaagaagag ctgcaacgcg aaaccttgtc gattgtgggc
    33661 tggcgtgatg tccccactaa cgaaggcgtg ctgggtgaaa tcgccctctc ctctctgccc
    33721 cgcattgagc aaattttcgt taacgctccg gcaggctggc gtccgcggga tatggagcgc
    33781 cgtctgttta tcgcccgccg ccgcattgaa aagcgtctcg aagccgacaa agacttctac
    33841 gtctgtagcc tgtcgaatct ggtgaacatc tataaaggtc tgtgtatgcc ggcggatctg
    33901 ccgcgctttt atctggatct tgcggacctg cgtctggaat cggccatctg cctgttccac
    33961 cagcgcttct ccactaacac cgtaccgcgc tggccgctgg cgcagccgtt ccgctacctg
    34021 gcgcataacg gtgaaatcaa caccatcacc ggtaaccgcc aatgggcgcg tgcgcgtacc
    34081 tataaattcc agacgccgct tatccctgat ctgcacgacg ccgcaccgtt cgtcaacgaa
    34141 accggctctg actccagttc gatggataac atgctggaac tgctgctggc aggcgggatg
    34201 gatatcatcc gcgccatgcg tctgttagtg ccacccgcct ggcagaacaa cccggatatg
    34261 gacccggaac tgcgtgcctt ctttgacttt aactccatgc atatggagcc gtgggatggc
    34321 ccggcgggca tcgtgatgtc cgacggtcgt tttgccgcct gtaacctcga ccgtaacggt
    34381 ctgcgtccgg cgcgctacgt catcaccaaa gataagctca tcacctgcgc ctctgaagtc
    34441 ggtatctggg attaccagcc tgacgaagtg gtcgaaaaag gccgcgtcgg gccaggcgaa
    34501 ctgatggtta tcgacacccg cagtgggcgt attctgcact cggcagaaac cgatgacgat
    34561 ctgaaaagcc gccatccata taaagagtgg atggagaaaa acgtccgccg actggtaccg
    34621 tttgaagatc tgcccgatga agaagtgggt agccgcgaac tggacgacga cacgcttgcc
    34681 agctaccaga aacagtttaa ctacagcgcg gaagagctgg actccgtaat tcgcgtactg
    34741 ggcgaaaacg gtcaggaagc ggtcggttcg atgggcgatg ataccccatt cgccgtgctc
    34801 tccagtcagc cgcgcattat ttacgactac ttccgccagc agtttgccca ggtgactaac
    34861 ccgccaatcg acccgctgcg tgaagcgcat gttatgtcgc tcgccaccag tatcggtcgt
    34921 gaaatgaacg tcttttgcga agcagagggc caggcgcacc gtttaagctt taaatcgccg
    34981 attctgctct actccgattt caaacagctc acgacgatga aagaggagca ctaccgcgca
    35041 gatacgctgg atatcacctt tgacgtcact aaaaccacgc tcgaagcgac agtcaaagag
    35101 ctgtgcgaca aagccgaaaa aatggtacgt agcggcaccg tgctgctggt gctctccgac
    35161 cggaatatcg ctaaagatcg cctgccggtt ccagccccga tggcggttgg cgcgatccag
    35221 acccgtctgg tcgatcaaag cctgcgttgc gatgccaaca tcatcgtcga aaccgccagc
    35281 gcccgcgatc cgcaccactt cgccgtgttg ctgggcttcg gcgcgacggc tatttatcca
    35341 taccttgcct atgaaacgct gggccgcctg gtagacaccc atgcgattgc caaagattat
    35401 cgtaccgtga tgctcaacta ccgtaacggc atcaacaaag gcttgtacaa aatcatgtcc
    35461 aaaatgggca tctccaccat cgcctcttac cgctgctcga aactgtttga agcggtcggt
    35521 ctgcacgatg atgtagtggg cctgtgcttc cagggggcgg tcagccgcac tggtggggcg
    35581 agctttgaag acttccagca ggatctgctg aacctgtcga aacgtgcctg gctggcgcgt
    35641 aagcccatca gccagggcgg cctgctgaaa tacgtccacg gcggcgaata ccacgcttac
    35701 aacccggacg tggtgcgcac gctgcaacag gcggtacaaa gcggcgagta cagcgactat
    35761 caggaatacg cgaagctggt taatgagcgt ccggcaacca cgctgcgcga tctgctggca
    35821 attacgccgg gtgaaaacgc ggtcaacatt gctgatgttg aaccggcaag cgaactgttt
    35881 aaacgctttg ataccgccgc gatgtctatc ggcgcgttaa gcccggaagc ccacgaggcg
    35941 ctggcggaag cgatgaatag catcggcggt aactcgaact ccggtgaagg cggcgaagac
    36001 ccggcgcgct atggcactaa caaagtgtcg cgcatcaagc aggtggcttc tggtcgcttc
    36061 ggcgttactc cggcgtatct ggtcaatgcc gacgtcattc agattaaagt cgcccagggc
    36121 gcgaagccgg gcgaaggcgg tcagttgccg ggcgacaaag tcactcctta catagccaaa
    36181 ctgcgctatt cggtgcccgg agtgacgctg atctccccgc cgccgcacca cgatatctac
    36241 tctatcgagg acttagcgca gctcattttc gacctcaagc aggttaaccc gaaagcgatg
    36301 atctccgtga agctggtttc cgaaccgggc gtaggcacca tcgcgactgg cgtggcaaaa
    36361 gcttatgccg atttaatcac catcgcaggc tatgacggtg gcaccggcgc aagcccgctg
    36421 tcgtcagtga aatacgcagg ttgcccgtgg gagctggggc ttgttgaaac ccagcaggcg
    36481 ctggttgcca atggtctgcg tcataagatc cgtttgcagg tcgatggcgg cctgaaaacc
    36541 ggtgttgata tcatcaaagc ggcgattctc ggcgcagaaa gcttcggctt cggcactggc
    36601 ccaatggtcg cgctcggctg taaatatctg cgtatttgcc acctgaacaa ctgcgcaacg
    36661 ggtgtggcaa ctcaggatga caaactgcgt aagaatcact atcacggcct gccgttcaag
    36721 gtgacgaatt actttgagtt tatcgcccgt gaaacccgcg agctgatggc acagctgggg
    36781 gtaacgcggc tggtggatct gattggtcgc accgacctgc tgaaagagct ggacggtttc
    36841 accgccaaac agcagaaact ggcgctgtcg aagctgctgg agaccgccga accgcatcca
    36901 ggtaaggcac tctactgcac cgaaaacaac ccgccgtttg ataacggcct gctgaacgcg
    36961 cagttgctgc aacaggcgaa accgtttgtc gatgaacgcc agagcaaaac cttctggttc
    37021 gatatccgca ataccgatcg ctccgtgggc gcgtcgcttt caggctatat cgcccagacg
    37081 cacggcgatc aggggctggc agccgatcct atcaaagcgt acttcaacgg caccgcaggc
    37141 cagagtttcg gtgtatggaa cgcgggcggc gtggaactgt acctgaccgg ggatgccaac
    37201 gactatgtcg gtaaaggcat ggcgggcggc ttaatcgcca ttcgtcctcc ggttggttcc
    37261 gccttccgca gccatgaagc aagtattatc ggcaacacct gcctgtatgg cgcgaccggt
    37321 ggtcgtctgt atgccgcagg ccgcgcgggt gaacgtttcg gcgtgcggaa ctccggtgct
    37381 atcaccgtgg tagaaggcat tggcgacaac ggctgtgaat atatgacggg cggtattgtc
    37441 tgtattctgg gtaaaaccgg cgttaacttc ggtgcgggca tgaccggcgg cttcgcttac
    37501 gttctcgatg aaagcggtga tttccgcaaa cgcgttaacc cggaactggt cgaggtctta
    37561 aacgttgacg atctggcgat ccatgaagag catctgcgcg gtcttatcac cgagcatgtt
    37621 cagcatactg gctctcagcg cggtgaagag attctggcga actggtcaac cttcgccact
    37681 aaatttgcgc tggttaaacc gaagtccagt gatgtaaaag cactgctggg tcaccgtagt
    37741 cgtagcgcag cagagttgcg cgtgcaggcg cagtaagggg tagcaacaat gagtcagaat
    37801 gtttatcaat ttatcgacct gcagcgcgtt gatccgccta agaaaccgct gaagatccgc
    37861 aaaattgagt ttgttgaaat ttacgagccg ttttccgaag gccaggccaa agcgcaggct
    37921 gaccgctgcc tgtcgtgcgg caacccatac tgcgagtgga aatgtccggt acacaactac
    37981 atcccgaact ggctgaagct cgccaacgag gggcgtattt ttgaagcggc ggaactgtcg
    38041 caccagacca acaccctgcc ggaagtttgc ggacgagtct gcccgcaaga ccgtctgtgc
    38101 gaaggttcct gcactctgaa cgatgagttt ggcgcggtga ccatcggcaa cattgagcgt
    38161 tatatcaatg ataaagcgtt cgaaatgggc tggcgtccgg atatgtccgg cgtgaagcaa
    38221 accggtaaaa aagtggcgat tatcggcgca ggcccggcag gtctggcgtg tgcggatgtc
    38281 ctgacgcgta acggcgtaaa agccgttgtc ttcgaccgtc atccagaaat tggcgggctg
    38341 ctgaccttcg gtattccggc cttcaagctg gaaaaagagg taatgacgcg tcgccgtgaa
    38401 atcttcaccg gcatgggtat tgaattcaaa ctcaatactg aagtgggccg cgacgtgcag
    38461 ctggacgatc tgctgagtga ttacgatgcc gtcttccttg gcgtcgggac ttatcagtca
    38521 atgcgcggcg ggctggaaaa cgaagacgcc gatggcgtgt acgcagcgct gccgttcctt
    38581 atcgccaaca ccaaacagtt aatgggcttt ggtgaaaccc gcgacgaacc gttcgtcagc
    38641 atggaaggca aacgcgtggt ggtccttggc ggtggcgaca ctgcgatgga ctgcgtgcgt
    38701 acgtccgtgc gccagggagc gaagcacgtt acctgtgcct atcgtcgtga tgaagagaac
    38761 atgccgggtt cccgccgcga agtgaaaaac gcgcgggaag aaggcgtaga gttcaaattc
    38821 aacgtccagc cactggggat tgaagtgaac ggtaacggca aagtcagcgg cgtaaaaatg
    38881 gtacgtaccg aaatgggcga accggatgcc aaaggccgtc gccgcgcgga gatcgttgca
    38941 ggttccgaac atatcgttcc ggcagatgca gtgatcatgg cgtttggttt ccgtccacac
    39001 aacatggaat ggctggcaaa acacagcgtc gagctggatt cacaaggccg catcatcgcc
    39061 ccggaaggca gcgacaacgc cttccagacc agcaacccga aaatctttgc tggcggcgat
    39121 atcgtccgtg gttccgatct ggtggtaacc gctattgccg aaggtcgtaa ggcggcagac
    39181 ggtattatga actggctgga agtttaagct ctgtcccagc gccgcctccc cagaaaagag
    39241 tcggcgctga aattctcctt tctttctgga aagcgcctcg cataatctga agaaaaccaa
    39301 tgtaatacgc tcatctggat tgttaccggt agatgcccgg cgtatagctg attccattat
    39361 tcaacgcagc gccatataaa gggacagata atggaatcgc tctcagagag aacctcaaca
    39421 ggctaccagc aaatccacga cggtattatt cacctggtcg atagcgcccg gacggaaacg
    39481 gtacgtagcg ttaacgcgct gatgaccgcg acatactggg aaattggccg acgaattgtc
    39541 gaatttgaac aaggtggcga ggcaagggct gcgtatggtg cgcagctaat c
//
LOCUS       DAJTMK010000016        38629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTMK010000016 DAJTMK010000000
VERSION     DAJTMK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38629
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.016"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..379
                     /locus_tag="OO135_000710"
     CDS             <1..379
                     /locus_tag="OO135_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5396659.1"
                     /translation="ICSNKHPRITDNSTYEQFMAEEFTQLISKSAGVDDIQMEIDEKF
                     MNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYI
                     SYNKSSLNNLVFSRFIDRLNESF"
     gene            526..1746
                     /locus_tag="OO135_000711"
     CDS             526..1746
                     /locus_tag="OO135_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5396660.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGSKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQAPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1719..2348
                     /locus_tag="OO135_000712"
     CDS             1719..2348
                     /locus_tag="OO135_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5396661.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2349..3498)
                     /gene="ybdL"
                     /locus_tag="OO135_000713"
                     /pseudo
     CDS             complement(2349..3498)
                     /gene="ybdL"
                     /locus_tag="OO135_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3607..4695
                     /gene="hcxA"
                     /locus_tag="OO135_000714"
     CDS             3607..4695
                     /gene="hcxA"
                     /locus_tag="OO135_000714"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5396662.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4705..4902)
                     /locus_tag="OO135_000715"
     CDS             complement(4705..4902)
                     /locus_tag="OO135_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5396663.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5084..7189)
                     /gene="cstA"
                     /locus_tag="OO135_000716"
     CDS             complement(5084..7189)
                     /gene="cstA"
                     /locus_tag="OO135_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5396664.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWTPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7370..7783)
                     /gene="entH"
                     /locus_tag="OO135_000717"
     CDS             complement(7370..7783)
                     /gene="entH"
                     /locus_tag="OO135_000717"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5396665.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7786..8532)
                     /gene="entA"
                     /locus_tag="OO135_000718"
     CDS             complement(7786..8532)
                     /gene="entA"
                     /locus_tag="OO135_000718"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5396666.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8532..9389)
                     /gene="entB"
                     /locus_tag="OO135_000719"
     CDS             complement(8532..9389)
                     /gene="entB"
                     /locus_tag="OO135_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5396667.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYSKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9403..11013)
                     /gene="entE"
                     /locus_tag="OO135_000720"
     CDS             complement(9403..11013)
                     /gene="entE"
                     /locus_tag="OO135_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5396668.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQRLASRASA"
     gene            complement(11023..12198)
                     /gene="entC"
                     /locus_tag="OO135_000721"
     CDS             complement(11023..12198)
                     /gene="entC"
                     /locus_tag="OO135_000721"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR5396669.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHIPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12573..13529
                     /gene="fepB"
                     /locus_tag="OO135_000722"
     CDS             12573..13529
                     /gene="fepB"
                     /locus_tag="OO135_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5396670.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13533..14783)
                     /gene="entS"
                     /locus_tag="OO135_000723"
     CDS             complement(13533..14783)
                     /gene="entS"
                     /locus_tag="OO135_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5396671.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLVLFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14894..15898
                     /gene="fepD"
                     /locus_tag="OO135_000724"
     CDS             14894..15898
                     /gene="fepD"
                     /locus_tag="OO135_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5396672.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGCGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15895..16887
                     /gene="fepG"
                     /locus_tag="OO135_000725"
     CDS             15895..16887
                     /gene="fepG"
                     /locus_tag="OO135_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5396673.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16884..17699
                     /gene="fepC"
                     /locus_tag="OO135_000726"
     CDS             16884..17699
                     /gene="fepC"
                     /locus_tag="OO135_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5396674.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17696..18829)
                     /gene="fepE"
                     /locus_tag="OO135_000727"
     CDS             complement(17696..18829)
                     /gene="fepE"
                     /locus_tag="OO135_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5396675.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKALDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAY
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19044..22925)
                     /gene="entF"
                     /locus_tag="OO135_000728"
     CDS             complement(19044..22925)
                     /gene="entF"
                     /locus_tag="OO135_000728"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5396676.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCEVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22922..23140)
                     /gene="ybdZ"
                     /locus_tag="OO135_000729"
     CDS             complement(22922..23140)
                     /gene="ybdZ"
                     /locus_tag="OO135_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5396677.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(23143..24345)
                     /gene="fes"
                     /locus_tag="OO135_000730"
     CDS             complement(23143..24345)
                     /gene="fes"
                     /locus_tag="OO135_000730"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5396678.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24588..26828
                     /gene="fepA"
                     /locus_tag="OO135_000731"
     CDS             24588..26828
                     /gene="fepA"
                     /locus_tag="OO135_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5396679.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSRVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27003..27623
                     /gene="entD"
                     /locus_tag="OO135_000732"
     CDS             27003..27623
                     /gene="entD"
                     /locus_tag="OO135_000732"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5396680.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27745..27897)
                     /gene="hokE"
                     /locus_tag="OO135_000733"
     CDS             complement(27745..27897)
                     /gene="hokE"
                     /locus_tag="OO135_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5396681.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(28248..28400)
                     /gene="hokE"
                     /locus_tag="OO135_000734"
     CDS             complement(28248..28400)
                     /gene="hokE"
                     /locus_tag="OO135_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5396682.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28854..29972
                     /locus_tag="OO135_000735"
     CDS             28854..29972
                     /locus_tag="OO135_000735"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5396683.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYYLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITGPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLEKKHCEIWAGD"
     gene            30038..30286
                     /locus_tag="OO135_000736"
     CDS             30038..30286
                     /locus_tag="OO135_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5396684.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            30351..30719
                     /locus_tag="OO135_000737"
     CDS             30351..30719
                     /locus_tag="OO135_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5396685.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSEPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRALIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30813..31466
                     /gene="nfsB"
                     /locus_tag="OO135_000738"
     CDS             30813..31466
                     /gene="nfsB"
                     /locus_tag="OO135_000738"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5396686.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADSRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31574..32821
                     /gene="ybdG"
                     /locus_tag="OO135_000739"
     CDS             31574..32821
                     /gene="ybdG"
                     /locus_tag="OO135_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5396687.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(32889..34265)
                     /gene="pheP"
                     /locus_tag="OO135_000740"
     CDS             complement(32889..34265)
                     /gene="pheP"
                     /locus_tag="OO135_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5396688.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34367..37510)
                     /gene="cusA"
                     /locus_tag="OO135_000741"
     CDS             complement(34367..37510)
                     /gene="cusA"
                     /locus_tag="OO135_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5396689.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37522..>38629)
                     /gene="cusB"
                     /locus_tag="OO135_000742"
     CDS             complement(37522..>38629)
                     /gene="cusB"
                     /locus_tag="OO135_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5396690.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         9306 a        10755 c         9811 g         8757 t
ORIGIN      
        1 aatttgtagt aataagcatc cgcgaattac tgacaatagc acttatgaac aatttatggc
       61 agaagagttc acccaattaa tatccaaatc agcaggagtt gatgatattc aaatggaaat
      121 agacgagaag tttatgaatc gcaaaatttc atttcgtggt tcctctttgc tgacaataat
      181 taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt ataactccca
      241 ccgtgacttt cttaatttaa aagagataaa actagaacat ccgttacctt caataaagct
      301 ttacatttcc tataataaat cttctttaaa taatttggtg ttctcccgat ttattgaccg
      361 tctaaatgag agtttttgat atataactac agacagctac tataactttc atctatttat
      421 tcacagcgcg aatgaaaaat aaaacactct gctttacagt gtttacttta taattaacag
      481 tcttatctca ctgttcaccc tgtaacgttc agagagcgtc ttcagatgtc tatttataaa
      541 attcctcttc cgctcaatat tctggaagct gcacgagagc gtattacctg gacactcaac
      601 accctacccc gcgtttgtgt ctcattttct ggcagcaaag actctggttt aatgctgcat
      661 ctgacagccg aacttgcccg acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg
      721 gaggcgcaat tctcttgcac tattaactat gttcagtccc tgcgcgagtt gtacaccgat
      781 gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa
      841 taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca ggccccgcaa
      901 gatgcgataa ccgatcctga ctttttctgc ttttaccagc caggcatgac cttcgaacaa
      961 tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat gatgatcggc
     1021 atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt
     1081 tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg gtacatttac
     1141 cccatttacg actggaaagt ggctgatatc tggacctggt atgcaaatca tcaaagtctc
     1201 tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt
     1261 tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt tatcgaaccg
     1321 gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc
     1381 ggacatgaca atcatttcta tgggcatcgg aaaatcctca agccagaaca tttagactgg
     1441 caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga gcattaccgc
     1501 aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgaggt tccacaaacc
     1561 cagcaagggg acattggcgc gaaagatatc ccctcctggc ggcggatatg caaagtttta
     1621 ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc gaagaactat
     1681 cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac
     1741 gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat cgcatcaaag
     1801 cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa gagccagtgg
     1861 attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac ccaaacaacg
     1921 tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga aatagatggt tttacccaac
     1981 ccattgtggt cacacatacg gataaaaatg ccatggaaat cgtggatggt tttcaccgcc
     2041 atgaaattgg taaaggctca tcatcattaa aattacgctt aaaaggttat ttgccggtga
     2101 cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat aatcgcgctc
     2161 gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg tgaacttagc cagttgggat
     2221 gggacgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg ctgcgcctga
     2281 agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca atattcccgc gcctggacgg
     2341 ttaaatagct aaagctggcg caggcgttca gctgctgcca gcaacgtcga ttccttcttg
     2401 gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac cgacagcgga
     2461 atcgccgcca cgccgtgctc ccgcgtcagc cactggcaaa actcaacatc atccagggta
     2521 gaaacctcgc tgtaatccac cagcaaaaag tatgtacctt cacacggtaa aatctccagc
     2581 cggctttcat ttaaagcatt caccagaata tcgcgcttct ggcgataaaa gtccggtaac
     2641 gcaagataat gctcaggttc tgcacgtagc atatcagcaa gcgccagctg tgccggggta
     2701 ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt cggcgctgat gggcgctggc
     2761 gcaacacaat aacccacttt ccagccggtc atatgataga tcttgccaaa tgaagaaacc
     2821 gccactgccc gctcacgcag ctgcggatgc gccagcacac tggcatggcc ctgttgtgaa
     2881 aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc
     2941 tgccacaaag cggcgaaatc agcctgctgc cagacagttg cactggggtt atgcggagtg
     3001 ttgaggatca ccagtctggt gcgctcgctt aataatgcgg caaattcctg ccagtcaagg
     3061 tggttgcagt gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact
     3121 gtcatagctg ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc
     3181 cgcgtataac gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg
     3241 atagccatac aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt
     3301 catgggcgcg tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata
     3361 gcgcggacca tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc
     3421 cagcgcgctc atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat
     3481 cagagggtta tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa
     3541 cacgatgcta gcatttggaa atcaagacgt ttagatgtct aaataaaaca ataaggacaa
     3601 cacaacatgc ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat
     3661 ccaagaagtt tcaatcacct gaacgatttt ttcactgatg aacaactttc tcgcgcggtg
     3721 tggatctacg gcaaacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga
     3781 cttccagggg caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa
     3841 ctggcggctg agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcactg
     3901 ctcgacaccg cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgttccgacg
     3961 atcgccgcta cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag
     4021 gcgctgcatt atgagatttt cgacgacgcc aattttatgg tgctggtgga accggagatt
     4081 atcctcaatg caccgcaaca atatctgctg gcggggatcg gtgacacgct ggcgaaatgg
     4141 tatgaagcgg tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcggctgggg
     4201 atcaataatg cgcaggccat tcgcgacgtc ttgttaaaca gtagcgaaca ggcacttgcc
     4261 gatcagcaaa atcagcagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct
     4321 ggtggtggga tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggctcatgcc
     4381 gtgcataacg gtctgactgt cctgccgcaa accgagaagt ttcttcacgg caccaaagtc
     4441 gcttacggaa ttctggtgca aagcgccttg ctgggtcagg atgatgtgct ggcgcaatta
     4501 accggggcgt atcagcgttt tcatctgccg actacgctgg cggagctgga agtggatatc
     4561 aataatcagg cggagatcga caaagtgatt gcccacacct tgcgcccggt ggagtccatt
     4621 cattacctgc cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa
     4681 tcgtttaaag cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg
     4741 cgcgtcctgc cgctcacgga aaaactcctc gtaggtcatc ggcgtttgat cgggatggtt
     4801 aacccgcata tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc
     4861 ctgacctaaa tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca
     4921 tgttgctgaa agagtgccgg atgcggcgtg aacgctttat ccggcctaca aatgctatca
     4981 acgcactcgg tcagtcccct cgccccatcg gggagagggt tagggtgagg gggaaccctc
     5041 ggcactatgc caacattcgc caacatcccc cctcactctg actttagtgc gcgcctttcg
     5101 cctgcgctac gatctcctcg acattttccg gcatcggctc atacggcgtt tctttcgccg
     5161 ttggcttcgg atctttcaat gccgcaagtg ccgtcttaat cgagaacagt gccagaacca
     5221 ccacgaccac cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt
     5281 gcgccagctg tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga
     5341 acttgttagc aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc
     5401 cagcggtcag ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac
     5461 gttgacgttt catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca
     5521 tctggttggc aataccaaac agcggccaca gagtgttaat accgcccagc ggatcgacca
     5581 cgccctgatg gaggaagtag ccccacgcca gtacgcacag cgccgttgcc agcaggttag
     5641 caggcagtga atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca
     5701 taaagcgcgc agcacgcgta cccgcatcta ccgccgtcag aataaacaga gcttcaaaca
     5761 gaatggcgaa gtgataccag aacgccacat ccatcatacc acccagcgca ccgtgcagga
     5821 tgtaagccat ccccaccgcc aacgtcggcg caccgcccgc acgggaaatg atcgactgtt
     5881 cacccacttc gctggcaatc tggttgagcg tatctggtgt aatgctaaag ccccagctac
     5941 tcaccacctg cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca
     6001 ttgggctgtt catggcaaag taaacgcccg gatcgatgat acaggcggaa accagcgcca
     6061 taatcgccac gaaggattcc attaacattc caccgtagcc gataaaacaa gcctgccctt
     6121 cgttcgccag catcttcggc gtggtgccgg aagagatcag cgcatggaag ccagacaccg
     6181 cgccacaggc gatggtgata aacaggaacg ggaacaggtt accggtccat accgggccag
     6241 tgccatcgac aaatttggtc agcgcaggca tggtcagcgt cgggcgcata atcaaaatgc
     6301 ctaccgccag accgacgatc gtcccgattt tcaggaaggt agagaggtag tcacgcgggg
     6361 ccagcagcaa ccacaccggc agcaccgccg ccacaaaacc gtaacccacc agcatccagg
     6421 tcagctgcac gccggtaaag tcaaagtacg gtgtccaggt cggactttct gccacccagc
     6481 cgccagagat aatggcgaaa atcaggaata ccagaccgat gaccgacact tcaccaatac
     6541 gccccggacg cagatagcgc aggtagatcc ccataaacag cgccagcgga atggtgaacg
     6601 caacggtata cgttccccac gggctatgag ttagggcttt caccacgatc atcgccagta
     6661 ctgcaaggat aatgaccatg atcataaagc aggccaccag cgctatcacc ccggcggttg
     6721 gccccatctc ttctttgacc agctcaccca gcgatcgacc gtcacggcgc gtagaaacaa
     6781 acagcaccat gaaatcctgc accgcaccgg cgagaaccac cccggcgagt agccagatca
     6841 tccccggcag gtagcccatt tgcgctgcca gcaccggccc aaccaacgga cctgctccgg
     6901 cgatggccgc aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc
     6961 cgtcgttatg gcgcaccgct ggcgtcatac gcttcgggtc aaccgccagc acatttttgg
     7021 cgatatacaa cccataaaaa cggtaagcga tcagatagat acagaccgat gccaccacaa
     7081 tccacagcgc gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc
     7141 ccattacaga gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc
     7201 gtgagcgtgt ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca
     7261 acacggtaac tatgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga
     7321 tcactccgtt aaccgagttt tcctgcaatc tcatttaatt ctgtcgggct catcccaaaa
     7381 ctgccgtacc cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgacgattt
     7441 cccagctctg attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt
     7501 cagacaccgg gcgatgatgg gtggcgttaa gttccgtgcc caccacacac tgcccgtcac
     7561 gggtcatcat aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc
     7621 catgcagcag gccaaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca
     7681 catcatctcc cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg
     7741 tggcgttcag ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt
     7801 tgagccgcca tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag
     7861 gaacaaaatc gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag
     7921 tttaaactgc tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag
     7981 cgtgcgttgc atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct
     8041 acccgccagt tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc
     8101 ataagcactc ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc
     8161 cccgccccgc tgacggcgaa actggttcat ggtttgctgg aacaggttaa acgcaccgcc
     8221 gacgttaacc gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat
     8281 gcgcaaaatt cccgccgcat tgatcagcac gtccagtcgc tccgtttcag ctaacagtcg
     8341 ctgacatacc tgcgccacct gcccggcgtc ggcaacatcc attacttcgg tcgcaaaggg
     8401 atattgctcc tgagtgaacg cttgatcaaa acctgtaact ttcgctcccg cctcaacaaa
     8461 cgccagcgcc gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc
     8521 gctgaaatcc attatttaac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg
     8581 tttttcgcca gcatgacaaa gtcgatatca ccatgcactt tgcgccagcg cgccgccagc
     8641 gccatcatgc gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca
     8701 tcggactcgt ccagcaacgg caggataacc tcacgcagcg ccgctttgct ggcggggact
     8761 ggtgctggca gtaattcttc agtcatcacc acccggccag accgcccggc cacatatttc
     8821 agcgacatca aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt
     8881 ttaatatcgc gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc
     8941 ccggtaataa tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga
     9001 tgaaacgcgc tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga
     9061 tccaccacct tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac
     9121 agcgcccgat cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg
     9181 tgctgtttgc tgtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg
     9241 cagttctcgc cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca
     9301 cgttgcagtt caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt
     9361 gcgtaagcct gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga
     9421 cgccagcctc tgacttaatt gttttttatc gactttcccg accgccgtca gcggaagtga
     9481 atctacacac tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa
     9541 acgacgcacc tgcaccgcgc gcagcggctc ttttaccacc agataagcac agcttttttc
     9601 gcccatcagc tcatcttcca tgctcaccag tgcggcatag atcaccgccg gatggcgcag
     9661 cagcaggttt tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt
     9721 ctcgcgcccc tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca
     9781 gtaaaaaccg ttggcatcaa aggcgctggc attgtgctgc ggacttttgt aataaccgcg
     9841 gaaggtgtac ggcccgcgcg tcatcaggcg tccgacttcc ccttgcggca gtggatttcc
     9901 ttcggcatcg gcaacccaca cttcatcgtc cggacacatt gggtaaccct gggtatggat
     9961 aattttctcc gcgctatcat caagacgggt gtagttcacc agcccttccg ccatgccaaa
    10021 cacctgctgc aactggcagc caatctcagc gggaatacgc gccgcaaggg tggcagaaag
    10081 acgtgcgccg ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc
    10141 agccagcgcc tgcaaccaca ggctgactgc cggcggcacc agcgcggtga cgttcacctg
    10201 atgtttttca atcaatggga agcaaagcgt agcgctggga tcggccgcca gaacaaccgt
    10261 tcctccggca agaaagacgc ccagcgatcc tggcgaactc atggcgtagt tatgagccgc
    10321 cgggatcgcg cacaagtagc gcgtctgttg tgtgaactga caaatctcga cgctacgacg
    10381 cacgctgtag tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc
    10441 gccggaaagc tggaaatagg ccacttcatc agcaggtgat ggcgtggcag taaaaccgtc
    10501 tgccggatgg ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttgt gcagttgcac
    10561 cacgcgaatg gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag
    10621 cgcatgttgg cgatcggcaa tcagcaatgc gggttcaatc tggctggcat aggcgttcag
    10681 ttcactacgc tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagcgc
    10741 gaaaaaggta atatacaatt cagcgacgtt acccagttgt accagcgcgg tttcaccagg
    10801 tttaatgccc tgacggcgta aagtacacgc gaggttatcc gccgcctgat tcagctcccg
    10861 gtaactcaac tgtagctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt
    10921 cagaatgtcg gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa
    10981 ctcttccggc cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac
    11041 attcaacatg gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc
    11101 aggcacaatc cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcacagcg
    11161 gatggtcacc acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa
    11221 cagttcgcgg tcgaacggct ccagttcagc aataacctga gtcgcggcct gatgcgggaa
    11281 accgctcagc gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga
    11341 attcgcttta ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg
    11401 tggagaggag ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg
    11461 agtcaccagt tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc
    11521 gcgatcgagc acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa
    11581 acgctcgccg tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc
    11641 agccagcggg atatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa
    11701 tacgccacta tcaatggcgg cgtcagtggt gatatcaatc aaccgtgaca acaccacttt
    11761 gtcgacctgc ggcgtggcgg taagtgcggc agcgcgggca accatctgtt caaacgtggt
    11821 ttgttccgga attgcctggc gttccaccac attcagcgac tggctgcggg tgaaacggcg
    11881 ggcggaagct tgtttttcct gacgggagaa cgactgccag gattcaggaa tatacagcga
    11941 cgaaggctga cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg
    12001 cactttggca tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc
    12061 cccgttcaca gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta
    12121 cggcgacata aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc
    12181 agccagtgac gtatccatat catcctccac aaaataataa aggctttaat aatgattatc
    12241 atttatattt tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa
    12301 cgcaatctgc aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat
    12361 tgatggattc gcataagcgc aatgtgatgt cctgcgccgt tcttccccct ctcccttcca
    12421 gggagagggc tggggtgagg gtcaatattc gcaccagtgc tggctgttcc catcacccta
    12481 accctctccc caaaggggcg agggtacgga tagtgcgctt tgtcgaattt gtcattacgc
    12541 ccttaacctt attaataaca ggaagctgat ttgtgagact cgccccgctc taccgcaacg
    12601 cccttctatt aacaggactt ttgctttccg gaatagccgc agttcaggcc gccgactggc
    12661 cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg
    12721 tttccaccag cgtcaccctg accggctcac tgctggcgat tgatgctccg gtgattgcca
    12781 gcggcgcgac cacgccaaat aaccgcgtcg cggatgacca gggcttttta cgccagtgga
    12841 gcaaggtggc gaaagaacgc aaactacaac ggctctatat cggcgaaccg agcgccgaag
    12901 ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc
    12961 tggcgctgta tgatcagctt tctaccatcg ccccgacatt aatcatcaat tacgacgaca
    13021 aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg
    13081 cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac
    13141 cgccgcagcc ggtcactgct attgtctata ccgccgctgc acacagtgcc aatctctgga
    13201 cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaaattgc
    13261 ccacagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg
    13321 gagaaaatct ggctgcaggg ttaaatggcg agtcactatt cctgttcgcc ggtgatcaga
    13381 aagacgccga tgcgatttat gccaatccac tgctcgcgca cctgcccgca gtacaaaaca
    13441 agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag
    13501 tgctggaacg acttaaggcg ctgttttaag cattaactgt cggacgctgt cacctgcggc
    13561 ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac gccgataatc
    13621 aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc tcccagaccg
    13681 cctaacagcg ccgcgcctat ggcatcgccc gtcacgtttt gtgccgtcca caaaccgttg
    13741 attcgcccta acattgcttc cggcgtttgc gtttgcagca ttgtatattg cagcaacgaa
    13801 ctgaccgcac tcagccagcc gaacagcacc agacaaacca cgcctaaaat ccacatcggc
    13861 atcaggccaa ataaaccaat ggcgaggaac gatcccaacg tggagagcaa catcaacaat
    13921 cctggtcgcg cgctatgcgc cagtttccca ctggttaatg cgccaatagc cgcaccgagc
    13981 gggatcgccg catagagaaa accaatctct gccgccgaca tctgccagtt gtcagccagc
    14041 gccggataca gtacccgcac cgcgctcgcc atcgtcaata aaccacccag caacgcaatc
    14101 ccgcccacca gcgggctggc gagcagaaaa cgaaatcctg ccagtaatga tttcaacgga
    14161 tgctcgcgcg gctgcggtgg cggtggcaac gccggaaggc ttaacaacgg tagcaaggta
    14221 ataaacgtgc ccgccgccgc cagcccgtag ttccaggcta cgccaccggt cgccagcaat
    14281 aaaccgccaa tcatgggcga aatcaccgac cccagacgca cggtcaacat ggtgatcgcc
    14341 ccggcctgca ttaagttttc acgccctacc agtgctggtg tcgccgccaa tagcgctgta
    14401 acgccaagcg atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac
    14461 ggctccggca gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc
    14521 gccagcaaaa tcactttttt gcgctcatag cgatccgcca gcacaccgcc aaccatcagg
    14581 ccaacaaaca tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgc
    14641 gtcatcatct ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag
    14701 ataaaacgag cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta
    14761 agcagccagg attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt
    14821 cttaggctgc acatgctaac atatccaaat aagatcgata acgataatta atttcattat
    14881 catggaagtt cgtatgtctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt
    14941 gctgttatta ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc
    15001 cgcttccgta gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt
    15061 gctcgacgcg cgactgccgc gcacccttgc cggtttactg gcaggcggcg cgcttggcct
    15121 tgccggggcg ttgatgcaaa ccctcacccg aaatccactt gccgaccccg gcttgcttgg
    15181 cgtgaacgcc ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc
    15241 cgcgcaggaa caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc
    15301 ctttaccggc agtcagggct gcgggcagtt aagtccggtg cgtttaaccc tggcgggcgt
    15361 ggcgctggcg gcagtgctgg aagggctgac cagcggcatc gccctgctta atcctgacgt
    15421 ctacgatcaa ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt
    15481 aaaagtggtg ctgatcccgg tgctgatcgc cggagcaacc gcgctattac tgagccgcgc
    15541 gctgaacagt ttaagcctcg gcagcgacac cgcgacggcg ctgggcagtc gcgtggcgcg
    15601 cacacagtta attggtctgc tggcgattac cgtgctttgt ggtagtgcga cagcggtagt
    15661 tggcccgatt gcctttattg gcctgatgat gccgcatatg gcgcgttggc tggtgggtgc
    15721 cgatcatcgc tggtcgctgc ccgtaacgct gcttgctacc cctgccctgc tgctgtttgc
    15781 cgatatcatc gggcgggtga ttgtgcccgg tgaactgcgc gtttctgtgg tcagtgcctt
    15841 tattggtgca ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt
    15901 tacgtctctc gccgattact catcacctgt ttgctgctta tttccgcctg tgtggttgca
    15961 ggtatctggg gattacgcag cggtgccgtc acgctggaaa cctcgcaggt attcgccgcg
    16021 ctgatgggcg atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc
    16081 gtgctgatgg cgctgttgat tggcgcggca ctgggcgtca gcggtgcgat ttttcagtcg
    16141 ctgatgcgta acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc
    16201 ggcgtgctgg tggcgatggt gctgtttggt caggacctga cggctatcgc gctggcagca
    16261 atggtgggcg gcattgtcac ttctctgttg gtctggctgc tcgcctggcg taacggtatc
    16321 gacacctttc ggttgattat tatcggcatc ggcgttcgcg ccatgctggt ggcctttaat
    16381 acttggctgt tgctgaaagc gtctttagaa acggcgctaa cagcgggttt gtggaatgcc
    16441 ggatcgctca atggcctgac gtgggcaaaa acttcgcctt ccgcacccat cattatattg
    16501 atgctcattg ccgccgcctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat
    16561 accgcctgcg cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca
    16621 gtggtgctta ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc
    16681 gcgccgcaca ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg
    16741 ctgtgcggag cgctgttact gctggcggcc gatctctgcg cccaacaact gtttatgccg
    16801 tatcaacttc cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg
    16861 ttaattcagg agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt
    16921 taaccctggg atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg
    16981 gtcacttcac ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct
    17041 taagccgcct gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac
    17101 attacgccag taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc
    17161 cgggcgatat caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt
    17221 ttacccactg gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa
    17281 taactcatct ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt
    17341 ggatcgcgat ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct
    17401 ggctggatat cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga
    17461 aaggctatac cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc
    17521 atttgattgc attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca
    17581 ctgctgaact gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag
    17641 ccggaacgcc gcttgtggtg ccgctcgaac gaacggcacc ttcaaccgca aatagttaaa
    17701 ctaagtggtc tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca
    17761 cgctaccaca agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac
    17821 ccggaccgtc ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat
    17881 tcacatcgtt gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt
    17941 caccgttcag ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac
    18001 cgtatgcgcc gagagaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt
    18061 atacgggctt tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa
    18121 tgtttgcatc aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa
    18181 actgggtttt gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg
    18241 cagagatata atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg
    18301 taaaacttag cgtccaggag gtatacagtg acggttcatc ttttttctta ctggcattgt
    18361 catcaagcgc tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat
    18421 cgattttcgc ctcttttaat tggtccatca cataaggtga tgaacgcaag tactcttcca
    18481 gcaagctaac agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat
    18541 taacatcgag atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa
    18601 tggcttcggc gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga
    18661 tcagtaaacc tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc
    18721 ataaaacttc gattagatta agtaaatcaa tttcattact actgggcgac gccagaggat
    18781 actcggaaaa atgagcgtca cttccctgtt taatattcag tgatgacatg ggtataacct
    18841 gaaaatgagt ccaatagggg gaaatctcca cggcgctcac tttaccagtt ggtgagaatt
    18901 atccgaagct gaagtttgta aattccttcc actgaatgca acattcactc tatttaagag
    18961 aattattcat caaacctata aatcaaacaa ataaaaaacc cataattatt ttctgtaatt
    19021 atgggtttat agataatatt aatttacctg tttagcgttg cgcgaataat cggcccaatt
    19081 tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg atagatatcc
    19141 agctcggcaa tccagggcga ccaagcgcgt tcggggctca taccttcctg aagcgtgcgt
    19201 tcagcaacaa acagcgtcgc tttaccatca aacggtacgc tatgagcagt cgtcagcaga
    19261 cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga agtacttccc
    19321 tgctgtgccg ccaggaaggc ctcgcgttcg cggttaatct ccgccagcac ttccgggtcc
    19381 agaccattag cctctttttc ctgccagttt tgcgtttctg gcggccaggt atccagcaaa
    19441 ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatgcc ctgcgccagc
    19501 gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg ttcaagtaac
    19561 gttgccagat gcgcttcaca gacttcatcc agatttgccg cggtatgcat ggggccattg
    19621 gggcgcggcg actgaatgcc gataatcgac cattgtggat cgatataacg cgagagcacg
    19681 ctaaactgcc aggcaaaacc ggatgccgga tggaaacaaa acagcgtcgg gccattacct
    19741 tcacgcaacg gcagaatggt ttcgaatccc atacgccggg tgctgtcttc ttcagcatca
    19801 ataatcgttg ccagtttagc gacggttgac gcgaccatca cttgccccgg cgtcacctgg
    19861 cgggcaacct gccgacttaa ctgcgctgcc agtttcattg ccagtagcga atgaccgcca
    19921 agcgcaaaga aatcagcatc ggcatcctgc acctcacaac ccagcaacga cgcgaatgcc
    19981 gcggcaataa tcgtttcact gcccgctttt ggcgcacgcc ccggcgcttg tgccttcagt
    20041 tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagagg taacagtgga
    20101 agttgcagca gaaccacggg caccatatgt ggtggcaatg tttcgcgaag ctgcgcctgt
    20161 aatgcgctgg tatccaacgg cagacctgat tgcgacacca gatagcccac caactgacgc
    20221 gcatcaccac cggtggctgc cgcctggtta atcacacagg cgtgggtaac ggcttgttcg
    20281 acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcaatacg ctgcccgcga
    20341 atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc cagccagcgg
    20401 gcaacgtctc cggtacggta catccgttca ccagggacaa aaggatcggc aataaagcgg
    20461 ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttgaatgcc ggtgagatag
    20521 agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat acgcagaccc
    20581 gtattccata ccggataacc aatcggcaca ctgctgccgc gcacctgtgc cagttcctcg
    20641 ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata tagattatgc
    20701 aacggcacgc tggttaactg ttgccattcg cggcataaat cagccggtaa ggcctcacca
    20761 ctacagaaaa cctgtttcaa cgtcgcgcaa ctctggcgag cggtttgcgg cgtcagcgat
    20821 gcaacaaatg ctgccagcat cgacggcaca aagtgcgtgg tcgttacgcc atattcggca
    20881 aagaattgct gcatagcgag cgggtcgcgg tgcgcttccg gttcagccat caccagttta
    20941 gccccggcaa taaacggcca gaaaaactcc cacaccgaga catcaaaact gcacggcgtt
    21001 ttttgggcaa cgacatcttc gcctgtaagc agataatgat tttgcatcca caacaggcgg
    21061 ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga gccagaggta
    21121 aagatgatat aagccgtgtg atgcggttgt gaaagttgca gcggcgcact gccctgcggt
    21181 ttaagcgggg cgttatagca aaggttggtt aaatctggaa catcggcaaa gcgcggcagt
    21241 tgatcgtcgg tggtgattaa cagcgacgga cgcgcatctt ccagcatcat tttcaggcga
    21301 tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac tatcgcatgt
    21361 agcgccaggg tcaaaaagac cgagcgcggt agcgccaccg ccacgctgtc gcccggttta
    21421 acgccacgct cacgcagcag attcgccagc gccaccacct gctcgcgcat ttcccgatag
    21481 ctgaactggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc cgcttgttct
    21541 gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt gatctgcgcc
    21601 agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag cgccggatcc
    21661 gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa cgttggctca
    21721 tcgtaacgct gtttattggc gaggatctca atactcaaat caccgtgttc atccgggaac
    21781 agggccagtt caaggtcatt aaccggaccg gttgccaagg tatgggtttg cgcctgaaca
    21841 ccaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc aaacagcggt
    21901 tcatcacctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta acgttgatga
    21961 cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag cgtttcttgc
    22021 gccgcaatgt gaatacccaa cggcaaaacg ttgagcacgg gtccggtagc cgtcagcgcc
    22081 gccgagccca gtcgacgcat aaagataaat ccagcggcgt agtccatgcg attgcacaat
    22141 cgccccagcc acaaagccgt cagcgcaagg gctaaatcgg tacgctgcac acctgaaagt
    22201 tgcgtagcca actggcggaa ttccccgtcg gtaaattcca ttttcagacg taaaatatct
    22261 gccgaagcgc tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg cggcagttga
    22321 cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc gcggtattgc
    22381 tggtactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt tggttcgcca
    22441 cgcagccatg tgcagtaaat attggcgatc tggcgggtaa tggccgggaa actgaagcca
    22501 tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc cacctgtatc
    22561 agctgatgaa agaccagtgg tttaccgcta tcgacgcgca gatcttgttg caaatccgcc
    22621 tgcattaatg cctgcgcagt accgtgcgga tcaatgttgg ttcgcaggtc gataatttct
    22681 ggcagttcga acgtcagcgc atcatcgacc cactgccaga cttcgccgtt atcttccgta
    22741 aaacgcatcc gcagcgtatc tgcttgcgct agtccgtcaa ccaccgcgcg ggccagtaat
    22801 ggcgcatcaa cctctccggt taactcaacg taatgcgcta cgctccaggc ggagggtaaa
    22861 tccgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg taaatgctgg
    22921 ctcattgcgc ctcctgcaac tgggtgaaat tcgccggtgt cagagtacgc cagtgggctt
    22981 caagccactg ctggcaggac tcttgtgact gcggctgaca cacaatgtcc cagcctgccg
    23041 gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc aaaatgtaaa
    23101 acgctccctg tggatcatcg aagggattac tgaatgccat attcaactcc tgtcatggaa
    23161 aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac aaagcgcatc
    23221 atgtccgccg tcaacctgac gccagaaaat ggattctttt atggggtgta attgcgcata
    23281 cagcgcctga ttggcccgca taatcatcgg ctcgcgaata cccgcttcca gcacaatgcg
    23341 cagaccttcg gcgctaactt caccagcttt aagcttttca agtaacacgc cctcttgctg
    23401 cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacaac caaaacgttc
    23461 aggccagtgc agtccggcat acagcgcgga aagtccacca aaactctgcc cggcaaccac
    23521 ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc accaggggta ataactcttg
    23581 ctgtactgcg agccagaaat ccgcattaca cggcagttcg tgagcgcggt gcgtggtgtc
    23641 gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg tcagcgaagt
    23701 cagcactggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca aaactgccag
    23761 cgggcgttct tctgctgttg catcgccggt ggtaaaaatc catacacgac gtgaattttt
    23821 caaccgttca cttttccaga taatttcttt ggctggtatt tctggcgctt gcggacaatc
    23881 ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt gccctcgccc
    23941 gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata gttttcgcca
    24001 gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat cgcgttcggt
    24061 gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc actgccagac
    24121 atcagtacct gcaatgcgct gcatcgactg gggctggctg ttctgatgat gatcggtcac
    24181 accagtgatg tagacccata cgcgctttat cggcgagtat tcttcagaac cttggggatc
    24241 acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt ccgggccatg
    24301 tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat aaccccatgt
    24361 ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg ataactattt
    24421 gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acattaatta accaactgca
    24481 ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg ttgaggacga
    24541 ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg aacaagaaga
    24601 ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag gcacaagagc
    24661 cgaccgatac tcctgtttca catgacgata ccattgtcgt taccgccgcc gagcagaact
    24721 tgcaggcacc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac ccggttgccc
    24781 gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt aactccacca
    24841 gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg gaaaacacgc
    24901 tgattttaat tgacggcaaa ccggtcagca gccgtaactc ggtgcgtcag ggctggcgtg
    24961 gcgagcgcga tacccgtggt gatacttccc gggtgccgcc tgaaatgatt gaacgtattg
    25021 aagttctgcg tggtccggct gcagcgcgtt atggcaacgg cgcggcgggt ggcgtagtta
    25081 acatcattac caaaaaaggc agcggtgagt ggcacggttc ctgggacgct tatttcaatg
    25141 cgccagaaca taaagaggaa ggtgccacta aacgcaccaa cttcagtctg accggcccgc
    25201 tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag gctgacgcgt
    25261 gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg acgttaccag
    25321 ccgggcgcga aggggtgatc aacaaagata ttaatggcgt ggtgcgctgg gacttcgcgc
    25381 ctctgcaatc actcgaactg gaagcgggtt acagccgcca gggtaacctg tatgcgggcg
    25441 atacccagaa caccaactct gacgcttaca ctcgctcgaa atatggcgat gaaaccaacc
    25501 gcctgtatcg ccagaactac tcgctgacct ggaacggtgg ctgggataac ggagtgacca
    25561 ccagcaactg ggtgcagtat gaacacaccc gtaactcgcg tattccggaa ggtctggcgg
    25621 gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat atcgatcttg
    25681 atgacgtgat gctgcacagc gaagttaacc tgccgattga tttcctcgtt aaccaaacgc
    25741 tgacgctggg tacggagtgg aatcagcaac ggatgaagga cttgagttcc aatacccagg
    25801 cgctgaccgg ggcgaacacc ggcggcgcta ttgatggtgt gagtgccacc gaccgtagcc
    25861 cgtattcaaa agcagaaatt ttctcgctat ttgccgaaaa caacatggag ctgactgaca
    25921 gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc aataactgga
    25981 gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctgaaa atgggcattg
    26041 cccgcgccta taaagcgccg agcctgtacc agactaaccc gaactacatt ctctacagta
    26101 aaggtcaggg ctgctatgcc agcgcgggcg gctgctatct gcaaggtaac gatgacctga
    26161 aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac ggctggctgg
    26221 cgggcgtcac ctggttccgt aacgattatc gcaataagat tgaagcgggc tatgtggctg
    26281 tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg aaagcagtgg
    26341 ttgaaggtct ggaaggatcg ttaaacgtac cagttagcga aacggtgatg tggaccaata
    26401 acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg tcgatcatcc
    26461 cggagtacac gttgaactca acgctgagct ggcaggcgcg ggaagatttg tcgatgcaaa
    26521 cgaccttcac ctggtacggt aagcagcagc cgaaaaaata caactacaaa ggtcagccag
    26581 cggttggccc ggaaaccaaa gaaatcagtc cttacagcat tgtgggtctg agcgcgacct
    26641 gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caacctgttc gacaaacgtt
    26701 tgtggcgtgc gggtaatgcc cagaccacgg gcgatctggc aggggccaac tatatcgcgg
    26761 gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtacatgagc gtaaacactc
    26821 acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc ggatgcggcg
    26881 tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag gaccatgtag
    26941 gcctgataag cgtagcgcat caggcagttt agcgtttgtc atcagtctct aatatggtcg
    27001 acatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat tttgttgagt
    27061 tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcattac gcgcaactgc
    27121 aacacgctgg acggaagcgc aaagccgaac atttagccgg gcggatcgct gcaatttatg
    27181 cgctgcggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc caaccggtgt
    27241 ggcctgcgga ggtatacggc agtattagcc actgcggggc tacggcattg gccgtggttt
    27301 cccgtcaacc gattggcgtt gatatcgaag aaattttttc tgcgcaaaca gcgaccgaat
    27361 tgacagacaa cattattaca ccagcagaac acgagcgact cgcagactgc ggtttagcct
    27421 tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag gcaagtgaga
    27481 tccaaactga tgcaggtttt ctggactatc agataattag ctggaataaa cagcaggtca
    27541 tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa aaaatagtca
    27601 taactctgtg ccaacacgat taattgacaa catctggtac gattcgcccg cagccatcac
    27661 tgaccgcggg cgaaagtgta aagcaggtgc cttaccactc tgacctgaca accggatatg
    27721 cggggattgc tccccgcaca acggctactt cttcggttcg taagcgagaa cagccttaaa
    27781 ctcaatatta cgttccttta cggtgaactc acacagcgag tctccgacca gaagcgtaaa
    27841 tcccagtacc gtcaaacaca gcactatgac tgccacaagg gcatatttcg tcagcatgtt
    27901 tatattgcct ccatgcgaag aagggactac catccgagtt gttgaggctc ggagtggcag
    27961 ccccaggatt gatagaaata tctcctgggg cttcatcttt ccagacctca gagttaacct
    28021 gaaaccggaa agctttaggc acccgccgtt atcttaccct tcctcaacaa aatagctgtt
    28081 tatttataca gtaaatcctg tttcggactt ctgtcggcaa aactcatcat tgctcttaaa
    28141 acgaaatcgc ccgcagccat cactgaccgc gggcgaaaat gtaaagcagg tgccttacca
    28201 tcctgacctg acaaccggat atgcggggat tgctccccgc acaacggcta cttcttcggt
    28261 tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa ctcacacagc
    28321 gaatccccga ccagaagcgt aaatcccagc accgttaaac acagcactat gactgccgca
    28381 agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac taccatccaa
    28441 attgttcagg tttgggtagg tagccccagg gttgatagaa atatcgtcct ggggcttcat
    28501 ctttccagac ctcagagtaa acctgaaacc ggaaagcttc aggcacccgc agttatctta
    28561 cccttgctta gcaaaatagc tgtttattta tacagtaagt cctgtttcgg acttctcact
    28621 ccccatttga tcaactgaag ctgaacccgt ttttttctgc atgcctcaat cgtcaaaatt
    28681 cttcgccacc agtcagcact gccataactt gtcacattgc aaaagtagcc aaattgcttc
    28741 tgtcataatc agttcaccat caccaaaaaa aagaaacatt ccagaacttc cgcatctcgc
    28801 ccagccgctc ccacttctat actgaataga aaacgccaac ataagagaaa cctatgccat
    28861 tacccgattt tcatgtttct gaacctttta cactcggtat tgaactggaa atgcaggtgg
    28921 ttaatccgcc gggctattac ttaagccagg actcttcaat actgattgac gcggttaaaa
    28981 ataagatcac ggccggagag gtaaagcacg atatcaccga aagtatgctg gagctggcga
    29041 cggatgtttg ccgtgatatc aaccaggctg ccgggcaatt ttcagcgatg cagaaagtcg
    29101 tattgcaggc agccgcagac catcatctgg aaatttgcgg cggtggcaca cacccgtttc
    29161 agaaatggca gcgacaggag gtatgcgata acgagcgcta tcaacgaacg ctggaaaact
    29221 ttggttatct cattcaacag gcgaccgttt ttggtcagca tgttcatgtt ggctgcgcca
    29281 gtggcgatga cgccatttat ttgctgcacg gcctgtcacg gtttgtgccg cacttcatcg
    29341 ccctttccgc cgcgtcgcca tatatgcaag gaacggatac gcgttttgcc tcctcacgac
    29401 cgaatatttt ttccgccttt cctgataatg gcccgatgcc gtgggtcagt aactggcaac
    29461 aatttgaagc cctgtttcgc tgtctgagtt acaccacgat gatcgacagc attaaagatc
    29521 tgcactggga tattcgcccc agtcctcatt ttggcacggt ggaggttcgg gtgatggata
    29581 ccccgttaac ccttagccac gcggtaaata tggcgggatt aattcaggct accgcccact
    29641 ggttactgac agaacgcccg ttcaaacatc aggagaaaga ttacctgctg tataaattca
    29701 accgtttcca ggcctgtcgc tatgggctgg aaggcgtcat taccggtccg cacactggcg
    29761 atcgtcgacc gctaacggaa gacaccttgc gattgctgga aaaaatcgcc ccttccgcac
    29821 ataaaattgg tgcatcgagc gcgattgagg ccctgcatcg ccaggtcgtc agcggtctga
    29881 atgaagcgca gctgatgcgc gatttcgtcg ccgatggtgg ctcgctgatt gggctggaga
    29941 aaaagcattg tgagatctgg gccggtgact aagtagggaa ttgccttaca gaagaatatc
    30001 cctgacaatg gtgttttaac ttacatttaa caatgttatg aaacaccctt tagaaacctt
    30061 gactaccgca gcaggcattt tgctgatggc tttcctctct tgcctgctgc tgcccgcccc
    30121 cgcactgggg ctgacgctgg cacaaaaact ggtgaccatg ttccatctga tggatcttag
    30181 ccagctttac actttattgt tttgtctgtg gtttttagtg ctgggcgcta ttgagtattt
    30241 tgttctgcgc tttatctggc gacgctggtt ctcgctggcg gattaaacgt cgattatcgc
    30301 cgtacttcgc ggcatacttt gcttattctc cttcgcctaa aggaacgttt atggataagc
    30361 aatcactgca cgaaacggcg aaacgcctgg cccttgagtt accctttgtc gagctttgct
    30421 ggccttttgg cccggagttc gatgttttta aaattggcgg caaaattttt atgctgtcgt
    30481 cggagctgcg cggcgtcccc tttatcaatc tgaagtccga accacaaaaa tccctgttaa
    30541 atcagcagat atacccgagc attaagccag ggtatcacat gaataaaaag cactggattt
    30601 cggtgtatcc cggcgaggaa atctccgaag cgttacttcg cgctctgatc aacgattcgt
    30661 ggaatctggt ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt ccaggctaaa
    30721 gcggaaatct atagcgcatt tttctcgctt accatttctc gttgaacctt gtaatctgct
    30781 ggcacgcaaa attactttca catggagtct ttatggatat catttctgtc gccttaaagc
    30841 gtcattccac taaggcattt gatgccagta aaaaacttac cccggaacag gccgagcaga
    30901 tcaaaactct cctgcaatac agcccatcca gcaccaactc ccagccgtgg cattttattg
    30961 ttgccagcac ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt aattatgtgt
    31021 tcaacgaacg taaaatactt gatgcctcgc acgtcgtggt gttctgtgca aaaaccgcga
    31081 tggacgatgc ctggctgaag ctggttgttg accaggaaga tgctgatagc cgctttgcca
    31141 cgccggaagc gaaagccgcg aacgataaag gtcgcaagtt cttcgctgat atgcaccgta
    31201 aagatctgca tgatgatgca gagtggatgg caaaacaggt ttatctcaac gtcggtaact
    31261 tcctgctcgg cgtggcggct ctgggtctgg acgcagtacc catcgaaggt tttgacgccg
    31321 ccatcctcga tgcagaattt ggtctgaaag agaaaggcta caccagtctg gtggttgttc
    31381 cggtaggtca tcacagcgtt gaagatttta acgctacgct gccgaaatct cgtctgccgc
    31441 aaaacatcac cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc ggctatttcc
    31501 cctcagattc tcctgatttg cataaccctg tttcagccgt catcataggc tgctgttgta
    31561 taaaggagac gttatgcagg atttaatatc ccaggttggg gatttagcgg gtattgagat
    31621 cgatcacacc acctcgatgg tgatgatttt cggtattatt tttctgaccg ccgtcgtggt
    31681 gcatattatt ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca tcgccagttc
    31741 acggctttgg ttgcaaatca ttacccagaa taaactcttc caccgtttag cttttaccct
    31801 gcaggggatt atcgtcaata ttcaggcggt attctggctg caaaaaggca ccgaagcggc
    31861 agatattctg actacctgcg cgcagttgtg gatcatgatg tatgcgctgc tttcagtctt
    31921 ctcgttgctg gatgttattt tgaatctggc gcagaaattc ccggcggcat ctcagttacc
    31981 gctgaaaggg atatttcagg ggattaaact gatcggcgca atcctggtcg gcattttgat
    32041 gatctcgctg ctgattggtc agtcgccagc gattctgatc agcggtcttg gtgcaatggc
    32101 tgccgtgctg atgttggtat ttaaagatcc gattcttggt ctggtggcag gtattcagct
    32161 ttccgcgaac gatatgctga aactgggcga ctggctggag atgccgaaat acggcgcgga
    32221 tggcgcggtg atcgatattg ggttaaccac cgtcaaagtg cgtaactggg acaataccat
    32281 taccactatt cccacctggt ctctggtttc tgactccttt aaaaactgga gcgggatgtc
    32341 agcatctggc gggcgacgta ttaagcgcag tatcagtatt gatgtcacca gtattcgttt
    32401 tcttgatgaa gacgaaatgc aacgtctgaa taaagcgcac ttgttaaaac cttatttaac
    32461 cagccgccat caggaaatta atgagtggaa tcgccagcaa ggttctacgg agtcgatatt
    32521 aaacctgcgc cgaatgacca atattggaac ctttcgtgcc tatctgaacg aatatctgcg
    32581 taaccatccg cggattcgta aagatatgac cttaatggta cgccaactgg ctccaggtga
    32641 taacggttta ccgctcgaga tctacgcgtt taccaacacc gtggtgtggc tggaatatga
    32701 aagcattcag gctgatatat tcgatcacat atttgcgatt gtcgaagagt ttggtctgcg
    32761 acttcatcag tcgccagccg gcaatgatat tcgctctctg gcgggtgcat ttaagcagta
    32821 attaaaaaaa ccgctctcat cgaatggatg agagcggaca tcgttttgtc ggatgtagcg
    32881 tgaatgcctt atttccgacg cagcgtttta aatgccacaa acaggaatac aatccacacc
    32941 ggcagcagga tcgctgacaa gcgcatatca tccatcgtgc acatcagcag caaaatcatg
    33001 ccgaggaagg caatgcaaag atagttgccg aacggataaa gcagcgcctt aaactgtgtt
    33061 tcacgtccct gacgtcgcat cgccgcgcga aaacgcagat gcgccaggca gatcataatc
    33121 cagttcaaca gcagcgttgc taccaccagc gccatcagca gaccaaacgc tttttgcggc
    33181 agcagatagt tgattaacac caccagcgaa gtgatcgctc cggaaagcat cagcgagtta
    33241 atcggcacgc cgcgatggct gacgcgagtc aaaaacttcg gcgcattacc ctgcacagaa
    33301 aggccaaaca gcatgcggct gttagagtaa accccgctgt tatacactga cagcgatgct
    33361 accagaatga cgaagttcag cgcagaagct acctcgttgc tgtcgagatt atggaaaatc
    33421 atcacaaacg ggctactgtt ggatttcact tccacccacg gatagagcgc cagtaaaacc
    33481 accagtgaac cgatgtaaaa cagcaggatg cgatacacca cctgatttac cgcttttgga
    33541 atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc cagaccgccg
    33601 aaggagaaca taattaccgc cagcgacaaa atcagcccat tccagccggt ggcgaagaaa
    33661 ccaccgtagc gccagaggtt gtcgatactg gctttctcgc cgccgtgacc agaaaacagc
    33721 agccacaggc caaagccgat cataccgatg attgccagca ctttaatcaa cgcaaaccag
    33781 aactcggttt cgccatataa gcgcacgttc accaggttaa cggcgttgat gataataaag
    33841 aaggcggcag cccaaatcca cgttggaaca tccgggaacc agtactgcat atagatgccc
    33901 gcagcggtca gctctgccat tcccaccagc acgaacatta cccagtagtt ccagccagag
    33961 aggaagcccg caaacggtcc ccagtattta taggcaaagt gggcaaatga accggatacc
    34021 ggctcttcga ccaccatctc gccgagctgg cgcataatca ggaaagcgat gatcccggcg
    34081 acgccgtagc ccagcaatac agccggaccc gccatctgaa tcgccgggcc aatgccaaga
    34141 aacagaccag taccaattgc gccacccagc gcaatcagtt gaatatgacg gttatgtaat
    34201 ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt ccgatacggt tgacgcgttt
    34261 ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca ccttttaaca tttagtgccc
    34321 atcgtcgcaa gacacaatcc acacggttaa acggggtatc ctgcttttat ttccgtaccc
    34381 gatgtcggtg cagccacatc agcttatacg ccgccgggat aataaacagc gacagcaaag
    34441 gcgcggtgat catgccgcca atcatcggtg cggcaatccg gctcatcacc tctgaaccag
    34501 cccccgttcc ccacagaatc ggcagcagac cggcgataat caccgccacc gtcatcgctt
    34561 tcgggcgcac gcgcagtacc gcgccgtgat acaacgcctc atccagcttc tgctcactga
    34621 atgtttgcgg attattcaac gacggctcgg cctctatggc gtgacgtaaa tacatcagca
    34681 tcaccacgcc aaattcggcg gcgcccccgg caagggcaat aaaaccggtg cccgttgcca
    34741 cagaaagatg aaagcccatc caccacagca accagatgcc gcccaccagc gcaaacggta
    34801 cgctgctgat aatcagcaac gcttcgccca cccgacggaa tgccagatac aacagcacga
    34861 agatgatcat cagtgtcatc ggcaccatga gtttcagctt atggttggcg cgctccagta
    34921 gctcgaactg cccggagaat gccacgctgg tgcccggttt taactgcact ttttcaacta
    34981 tcgctttttg caaatcgtga accaccgaca ccatgtcacg atcgcgggca tcgatataaa
    35041 tccagctcgt cgggcgcgca ttctcggttt tcagcatcga cggtccggtt gagactttaa
    35101 cgtcggccac gtctgccagg gtgatttgct gcttcatcgg cgtcaggatc ggtagctggc
    35161 gcagtgcctg cgggctatcg cgccagcttt gcggataacg cagattaatg ggataacggg
    35221 caatcccttc caccgtttcg ccaaccatcg ccccgcccac cgcagaagtc acaaacaact
    35281 gcacatccgc caccgtcata ccgtaacgcg cggctttttc acggttaatc tcaacgttga
    35341 tatagcgccc accttccaga cgctcggcaa gcgcagaagc cacgcctggc accgttcgcg
    35401 ctacttcttc aatttgctca gccatcgcgt cgatatccgc cagcacagtg ccggaaactt
    35461 taatgccgat ggggctttta atgccggttg agagcatatc gatacggtta cgaattggcg
    35521 gcacccacag attcgccagc cccggcagcc gcacggtgtt atccagttcc tcaatgattt
    35581 tgtccatcgt catgccaggc cgccactgat cctgcggctt aagctggatg gtcgtttcaa
    35641 ccatctcgag cggagcagaa tcggtggcgg tttccgcttt cccggttttg ccaaataccc
    35701 gcgccacttc aggtacgctc ataattagct tgtcggtttt ttgcagcata ctcgccgcct
    35761 ctgctgcgga aatccccggc agcgtcgatg gcatatacaa caagtcgcct tcattgatct
    35821 gcggtaaaaa ttccccgcca actttattga gcggccagag aaccgtcagc accgaaagcg
    35881 ctgccaccaa cagcgtggtt ttcggccagt gcagtacttt cagcaataga ggatgataaa
    35941 cacgaatcaa aaagcgattg agcgggttac tgctttccgg cggaattttg ccacggatcc
    36001 agtaacccat caggatcggg atcactacga tcgccagcag cgccgcaccc gccatcgcat
    36061 acgttttggt gaacgccagc gggccaaaca gacgcccttc ttgcccttcc agggtgaaga
    36121 ttgggataaa cgacagcgtg ataatcagca gactgataaa cagcgccggc cccacttcaa
    36181 cagatgcatc ggtaatcacc tgccagcgcg ttttattatc cagcgtggcg tcaggatgct
    36241 ggtgctgcca ctcttccagc cgtttatgcg cattctcgat catgacgata gcagcatcga
    36301 ccatcgcccc gacggcaatc gcaatgccac ccaacgacat aatattggca ttcagtccct
    36361 ggaagtgcat gacaataaaa gcaatacaca accccagcgg caacgaaata atcgccacca
    36421 gcgccgagcg cacatgccag agaaacagcg cacagactac cgccaccaca ataaactctt
    36481 ccagcaactt gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg cgatcgtatg
    36541 ttgtaactat ctccacgcct tccggcagac tacttttcag cgtttccagt ttgtccttca
    36601 cggcggcgat cacttctcgg gcgtttttgc cggagcgcag gatcaccacc ccgcccgcca
    36661 cttcgccttc gccgttcagt tcggcaatgc cccggcgcat ctccgggcca acctggacct
    36721 tcgcaacatc gcgcagataa acgggcacgc cattttcact ggcttttaaa acgatgtgat
    36781 taaagtcgtc gagcgtttgc agatagccgc tggcgcgcac catatattcc gcttccgcca
    36841 gttcgatcga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt tttacttcgg
    36901 cgagactgat gccatactgc gccaggcgct ggggatcgat aaccacctga tactctttca
    36961 ccacaccgcc caccgacgcc acttccgcaa cgtcagggat ggttttcagc tcatatttga
    37021 gaaaccagtc ctgtaatgag cgtaaatcgg ccagatcgtg cttaccgctg cgatccacca
    37081 gtgcatattc atagatccag ccaacacccg tggcatctgg ccccagctcg gcgctgactc
    37141 ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgaa cgcgcccagt
    37201 acggatcggt accatcttcg aaaatgacat acacataaga gtcgccaaac tgcgagaaac
    37261 cacgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga taagtcacct
    37321 gattttcaac gatttgcggt gcctgcccgg gatagctggt tttaataatc acctgcacat
    37381 cggagagatc cggcagcgca tccactggcg tattaatgat ggtccaggtg ccccagatgc
    37441 taagaaacaa cgcgcccatc agcaccaaaa aacggttcgc caccgagcga cgaataatcc
    37501 attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc gcatccgctc
    37561 cagtgcgcca gaaatattgg cttcggaatc aatcaggaaa aggccgctgg aaaccacctt
    37621 ttcaccttcc gccagaccag agcgtaatgc ggtgacacct tgcgatgcct ggaaaacggc
    37681 aacgcgtttc ggtacaaagc gcccgtcggc atcaatggta atcacccgct gttcgctacc
    37741 ggtatcaatc agcccttgtg acggaatgag cagcatcggt tcgctggcgg tattgagttg
    37801 caaccaggcg ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt ccagacgcag
    37861 ctgcagcgtg cgggtcgcgg catccacgcc gggtagcagc gtccatttgc ggatggtgag
    37921 tgttttatcc ggtcgtgccg gaacggtgag cgtaaactgc gaggcatctt tcaccagcca
    37981 ggcgatagac tccgggatcg cagcagtgac ccacaccggg tccataccct gaattttcgc
    38041 taccacgtta tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg tgatcacgcc
    38101 atcaatgggc gctttgagcg taaagcgagt ctggattttt tgcgtggcga tcaggcggcg
    38161 aatatccgcc tccggcattc ccgccagtcg cagccgctca agaatgcctt cagtctgggt
    38221 cgccgtaccg ccggtttcgc gcagcagtaa atactcactc tgcgcttcca cccagtcagg
    38281 aatggtcagg tcgagaagcg gtgtgccctt ttgtacttta tcgcccacgg taagcggata
    38341 caccttgtcg ataaacccgg cagcgcgagc ctgcacaatg gcatactgat actcgttgta
    38401 actgacattc gccgggaaac tctgggcaaa agtcagcggt ccgcgcgtga cggtagccgt
    38461 tttcaccccc agattctgcg tctgagtcgg gtcaatgcgc acaccagacg cagaactctc
    38521 ttcatcggca tatttcggca ccagatccat atccataaac ggcgatttac ctggtttatc
    38581 gaaccgcgta ttggggtaca tcgggtcgta ccagaataag attttacgt
//
LOCUS       DAJTMK010000017        38363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTMK010000017 DAJTMK010000000
VERSION     DAJTMK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38363
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.017"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..727
                     /locus_tag="OO135_000743"
     CDS             <1..727
                     /locus_tag="OO135_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5396691.1"
                     /translation="LEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPP
                     GWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHM
                     RFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQ
                     KQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLG
                     HKVQATTDRYNDTRGQEWVKLVI"
     gene            894..1688
                     /gene="potC"
                     /locus_tag="OO135_000744"
     CDS             894..1688
                     /gene="potC"
                     /locus_tag="OO135_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5396692.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            1685..2725
                     /gene="potD"
                     /locus_tag="OO135_000745"
     CDS             1685..2725
                     /gene="potD"
                     /locus_tag="OO135_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5396693.1"
                     /translation="MKKWSRHLLAAGALALGAAHADDNNTLYFYNWTEYVPPGLLEQF
                     TKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKSKL
                     TNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYKGS
                     LLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPYME
                     GEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLINFL
                     LRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAASSI
                     YEEYYQKLKAGR"
     gene            complement(2881..3701)
                     /gene="cobB"
                     /locus_tag="OO135_000746"
                     /pseudo
     CDS             complement(2881..3701)
                     /gene="cobB"
                     /locus_tag="OO135_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
     gene            complement(3717..4628)
                     /gene="nagK"
                     /locus_tag="OO135_000747"
     CDS             complement(3717..4628)
                     /gene="nagK"
                     /locus_tag="OO135_000747"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5396694.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYTANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERAHHGDAGGMRGAAFLHLTD"
     gene            complement(4657..5901)
                     /gene="lolE"
                     /locus_tag="OO135_000748"
     CDS             complement(4657..5901)
                     /gene="lolE"
                     /locus_tag="OO135_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5396695.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLYVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(5901..6602)
                     /gene="lolD"
                     /locus_tag="OO135_000749"
     CDS             complement(5901..6602)
                     /gene="lolD"
                     /locus_tag="OO135_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5396696.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(6595..7794)
                     /gene="lolC"
                     /locus_tag="OO135_000750"
     CDS             complement(6595..7794)
                     /gene="lolC"
                     /locus_tag="OO135_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5396697.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8056..9129
                     /locus_tag="OO135_000751"
     CDS             8056..9129
                     /locus_tag="OO135_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5396698.1"
                     /translation="MKQKELWINQIKGLCIYPVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9257..12703
                     /gene="mfd"
                     /locus_tag="OO135_000752"
     CDS             9257..12703
                     /gene="mfd"
                     /locus_tag="OO135_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5396699.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            12847..13808
                     /gene="ldtC"
                     /locus_tag="OO135_000753"
                     /pseudo
     CDS             12847..13808
                     /gene="ldtC"
                     /locus_tag="OO135_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(13891..14148)
                     /gene="bhsA"
                     /locus_tag="OO135_000754"
     CDS             complement(13891..14148)
                     /gene="bhsA"
                     /locus_tag="OO135_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5396700.1"
                     /translation="MKNVKILIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14389..15021
                     /gene="comR"
                     /locus_tag="OO135_000755"
     CDS             14389..15021
                     /gene="comR"
                     /locus_tag="OO135_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5396701.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVKSALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15083..15622)
                     /locus_tag="OO135_000756"
     CDS             complement(15083..15622)
                     /locus_tag="OO135_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5396702.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(15849..17153)
                     /gene="ndh"
                     /locus_tag="OO135_000757"
     CDS             complement(15849..17153)
                     /gene="ndh"
                     /locus_tag="OO135_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5396703.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(17553..18095)
                     /locus_tag="OO135_000758"
     CDS             complement(17553..18095)
                     /locus_tag="OO135_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5396704.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18118..19143)
                     /gene="nagZ"
                     /locus_tag="OO135_000759"
     CDS             complement(18118..19143)
                     /gene="nagZ"
                     /locus_tag="OO135_000759"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5396705.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19154..19978)
                     /gene="thiK"
                     /locus_tag="OO135_000760"
     CDS             complement(19154..19978)
                     /gene="thiK"
                     /locus_tag="OO135_000760"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5396706.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(19962..20600)
                     /gene="lpoB"
                     /locus_tag="OO135_000761"
     CDS             complement(19962..20600)
                     /gene="lpoB"
                     /locus_tag="OO135_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5396707.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(20614..20991)
                     /locus_tag="OO135_000762"
     CDS             complement(20614..20991)
                     /locus_tag="OO135_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5396708.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(20994..21353)
                     /gene="hinT"
                     /locus_tag="OO135_000763"
     CDS             complement(20994..21353)
                     /gene="hinT"
                     /locus_tag="OO135_000763"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5396709.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            21687..23812
                     /gene="fhuE"
                     /locus_tag="OO135_000764"
                     /pseudo
     CDS             21687..23812
                     /gene="fhuE"
                     /locus_tag="OO135_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(23872..25305)
                     /gene="ptsG"
                     /locus_tag="OO135_000765"
     CDS             complement(23872..25305)
                     /gene="ptsG"
                     /locus_tag="OO135_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5396710.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILVGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(25600..26397)
                     /locus_tag="OO135_000766"
     CDS             complement(25600..26397)
                     /locus_tag="OO135_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5396711.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(26408..27412)
                     /gene="holB"
                     /locus_tag="OO135_000767"
     CDS             complement(26408..27412)
                     /gene="holB"
                     /locus_tag="OO135_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5396712.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(27409..28050)
                     /locus_tag="OO135_000768"
     CDS             complement(27409..28050)
                     /locus_tag="OO135_000768"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5396713.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(28040..29062)
                     /gene="yceG"
                     /locus_tag="OO135_000769"
     CDS             complement(28040..29062)
                     /gene="yceG"
                     /locus_tag="OO135_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5396714.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQELELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(29065..29874)
                     /gene="pabC"
                     /locus_tag="OO135_000770"
     CDS             complement(29065..29874)
                     /gene="pabC"
                     /locus_tag="OO135_000770"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5396715.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(29994..31235)
                     /gene="fabF"
                     /locus_tag="OO135_000771"
     CDS             complement(29994..31235)
                     /gene="fabF"
                     /locus_tag="OO135_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5396716.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(31323..31559)
                     /gene="acpP"
                     /locus_tag="OO135_000772"
     CDS             complement(31323..31559)
                     /gene="acpP"
                     /locus_tag="OO135_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5396717.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(31770..32504)
                     /gene="fabG"
                     /locus_tag="OO135_000773"
     CDS             complement(31770..32504)
                     /gene="fabG"
                     /locus_tag="OO135_000773"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5396718.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(32517..33446)
                     /gene="fabD"
                     /locus_tag="OO135_000774"
     CDS             complement(32517..33446)
                     /gene="fabD"
                     /locus_tag="OO135_000774"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5396719.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(33462..34415)
                     /gene="fabH"
                     /locus_tag="OO135_000775"
     CDS             complement(33462..34415)
                     /gene="fabH"
                     /locus_tag="OO135_000775"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5396720.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLMLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(34483..35553)
                     /gene="plsX"
                     /locus_tag="OO135_000776"
     CDS             complement(34483..35553)
                     /gene="plsX"
                     /locus_tag="OO135_000776"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5396721.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIREASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(35634..35807)
                     /gene="rpmF"
                     /locus_tag="OO135_000777"
     CDS             complement(35634..35807)
                     /gene="rpmF"
                     /locus_tag="OO135_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5396722.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(35859..36380)
                     /gene="yceD"
                     /locus_tag="OO135_000778"
     CDS             complement(35859..36380)
                     /gene="yceD"
                     /locus_tag="OO135_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5396723.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            36579..37163
                     /gene="yceF"
                     /locus_tag="OO135_000779"
     CDS             36579..37163
                     /gene="yceF"
                     /locus_tag="OO135_000779"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5396724.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(37275..38234)
                     /gene="rluC"
                     /locus_tag="OO135_000780"
     CDS             complement(37275..38234)
                     /gene="rluC"
                     /locus_tag="OO135_000780"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5396725.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         9466 a        10266 c         9407 g         9224 t
ORIGIN      
        1 actggaagta agagactttg cattaatact ggatgagtgg ctggataaag acatggtcag
       61 cacagcgaga gtaaatcgtg gattatgggt tgatatttat aaagaagcac agcatgcagg
      121 ggaagttcct cctggatgga atcctccgga ggctacccgt aaaccgatcc ctaaagtaac
      181 cagagccagg ctcaccatgg aagactggca aaaaatttac aatgcaacgc ctgaaaaaca
      241 ctttatccgt aacgcaatgc ttcttgcgat tgttactggt cagcgccgtg atgacatttg
      301 ccacatgcgt ttttcagatg tgtggaacga acacttgcat atcacccagg gaaaaaccgg
      361 aatgcgtctg gcgttaccgc ttacactacg ctgtgatgcc attgggataa cgttaaaaga
      421 agttattgat gggtgccgag acagaatatt aagtccatat ctaatccata gtcggcacca
      481 gaaacaaccg aagccgatga gtaaagacaa cctgagcgac tactttgcca aagcacggga
      541 tctggctggg ataattccac cagcaggaaa aactccgcca acatttcatg aacaacgctc
      601 tctatcagaa cggctgtacc gtgcacaggg tatcgataca aaaacattac taggacataa
      661 agtccaggca accaccgatc gctataacga tactcgaggt caggaatggg ttaagctggt
      721 tatttgacaa acaccatatg gaatatccgt caccatctca ggaaaaaaat gttgtaaaat
      781 gcgtggccta gttttgggga aacgttttgg agagattttg gagaaaggaa aaaattgaat
      841 aaattcaaaa tcctgaaagt gctccacctt atgaataaga aggtggaact cgaatgatcg
      901 gtcgactgct tcgcggcggt tttatgaccg ctatctacgc gtacctgtat atcccaatca
      961 ttattttgat tgtgaactcc tttaacagct cgcgctttgg catcaactgg cagggtttta
     1021 ccaccaaatg gtatagcctg ctgatgaaca acgacagcct gttacaggca gcgcagcatt
     1081 cactaacaat ggcggtgttt tcggcgacgt ttgctacgct tatcggttca ctgacggcag
     1141 ttgcgctgta ccgttatcgc tttcgcggta agccgttcgt tagcggaatg ctgtttgtgg
     1201 taatgatgtc accagatatc gtgatggcaa tttcgctgct ggtgctgttt atgctgctgg
     1261 gtattcagct tggcttctgg tcgctgctgt tctcgcatat caccttctgc ctgccatttg
     1321 tggtggtgac ggtgtattcg cgcctgaaag gttttgacgt gcggatgctg gaagcggcga
     1381 aagatctcgg tgccagcgaa tttaccattc tgcgaaaaat catactgcca ctggcaatgc
     1441 cagcggtggc agcgggctgg gtgttaagct ttaccctgtc gatggacgat gtggtagttt
     1501 cttcgttcgt caccggacca agttatgaaa ttctgccgtt aaaaatttat tcgatggtca
     1561 aagtcggcgt atcgccggaa gttaacgcgc tggcaaccat attactggtg ctgtcgctgg
     1621 tgatggttat tgccagtcag cttattgctc gtgataaaac gaaaggtaac acaggggacg
     1681 ttaaatgaaa aaatggtcac gccacctgct cgcggcgggt gctctggcac tgggcgccgc
     1741 tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg
     1801 actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc
     1861 gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcgtatg acctggtggt
     1921 tccttcaacc tattacgtcg ataaaatgcg taaagaaggg atgatccaga agatcgacaa
     1981 gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc cttttgaccc
     2041 gaataacgac tactccattc cgtatatctg gggggcgacg gcgattggtg ttaacggtga
     2101 tgcggtggat ccgaaatctg tcaccagctg ggccgatctg tggaagccag agtacaaagg
     2161 cagcctgctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc gtaagctggg
     2221 ctattccggt aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa
     2281 actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg
     2341 cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac
     2401 gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat
     2461 cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc
     2521 ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg
     2581 taagctgtta agtccagaag tggcgaacga taaaacgctc tacccggatg ctgaaaccat
     2641 taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta
     2701 tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt
     2761 ttatttagca aggatgtggc taatgagtca ggacagtaga gttttatccc actctttcgt
     2821 cggtattgtg gtccgctcgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
     2881 ttataatccc ttcagcaact tttcaacaaa ctcaggcacc acctggcttg ccgggccgtt
     2941 atatttctcg gcaaattcat taccgacctg acttggttcg agattcagtt ccacggtgtg
     3001 cgcgccatgc agtttcgctt cgtgaacaaa cccagccgcc ggataaacat gcccggaagt
     3061 accaatggca atgaaaatat cagccatcga caacgccata taaatttcat ccatgccgag
     3121 tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg
     3181 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
     3241 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
     3301 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt
     3361 agccagcgca agatgcgcgg cgttaggctg aatttttggc tgctgcagct gtcgacgacg
     3421 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
     3481 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
     3541 ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
     3601 caccacttta tctctgaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt
     3661 ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa
     3721 tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gtgcgcgcgt
     3781 tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc
     3841 gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca
     3901 atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc
     3961 cttgcctgct catcgccttg attataaagc gcaataattt cgggagcctg caactgttga
     4021 tgataatagt gttgatacag ccacgcaaaa ccgcgaccag acagataatt ctcaatgcag
     4081 ccatgctgac cacagccgca gcgacgtaac gggaaatcca accccatcat ggttaacgca
     4141 tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt
     4201 ttgccgttga aaatcagccc accgccaacg ccggtaccaa gaatcaaccc catcaccagt
     4261 ggatattgcg taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta
     4321 tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg
     4381 gctgcgggga cattggcggt atacagcgta ccatcttctg tttccggcat tcccggtata
     4441 ccaatgccga cagaacctct acagccaaaa cgttgatcag cttcggctac cagctcgcac
     4501 actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc
     4561 aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg
     4621 taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc
     4681 agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa
     4741 tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat
     4801 aaaatagata tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat
     4861 cggggtaagt tgcagtgaaa caactacgcc gataatcagc ccacacaggc tgccgaacag
     4921 ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag
     4981 cgttcttaat actgcgatat cgccactctt gtctttcacc gccatcacta aggtggagac
     5041 gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat
     5101 catctggata tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata
     5161 gctgttggtc atttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat
     5221 tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag
     5281 cgggatcatg gcaaaactgt gatcgagttg gccactcaac tgcaaaatac ccgcaacgta
     5341 caaacgcaca cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcatgat
     5401 cgacacccaa tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat
     5461 ttgctgttcg cccgctttaa aattgcgcca cgcatccccc tgaacaaacg agggtaatgc
     5521 gctcagacgc tgttcctgtt gtgggttaac gcccttcacc tggattgcgc gcagattcgc
     5581 tccactttcc accagcccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac
     5641 tttctgcact ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcttcgat
     5701 ttcaccatgc ggcaccaccg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat
     5761 cgcgcttaag ccgacgatca ataccgccac accgagggca atgccaatgg tagaaatgac
     5821 ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga ctaaaacgca ggccaattaa
     5881 taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt
     5941 ccatcacgca tttctaactg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc
     6001 accaggaagg cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg
     6061 gcgttacgcg catcgaggtt accggtaggc tcatccgcca gtaccagacg cggattattg
     6121 accagcgcac gggcaatcgc tacacgctgg cgttcgccgc cagaaagttc agatgggcgg
     6181 tgattcgcac gatgctccag ccccaccgct tttaacatct ccagtgcacg gctgttgatt
     6241 tcagcgggct ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcggtaaaa
     6301 tccggcagca ggtggtgaaa ctgataaata aagcccagtt tctggttgcg cagttctgct
     6361 tttgctgccg acgacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt
     6421 ggcgtatcca gtccgcccag caggtgcagc aaggtgcttt taccggaacc agagctaccg
     6481 acgatcgcca tcatttcacc ttcgccgaca ctgaaactga cattgtgcag tacatcggtt
     6541 tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc
     6601 ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga
     6661 cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc
     6721 catcggcagc gccgcgccat caagcaggac gccgattatt ggcatcagat tatttaactg
     6781 gctggcaagc agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc
     6841 cccttgcacc ataaagatca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat
     6901 cgctacttcg ccctgcttct ccatcaccat cagccccagc gaggtaataa tgttaaacgc
     6961 cgcaacggcg acaatcaggc tcagcagtaa acccatcata tttttttcca tgcgtacggc
     7021 ctggaacaac tcgcctttac gatcacgcca gtcctgccat ttgctgtctt caggcagttt
     7081 ttgctgactt aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc agccggtaat
     7141 attgcctgcc ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcatt
     7201 gccatcgact tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg
     7261 aatacgcccc atcggcgtga actggctggc agatggtacc atcatgcgga tttgatcacc
     7321 gcgattaacg cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg
     7381 ctcgaggtca gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg
     7441 atcgataccc agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc
     7501 ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg
     7561 ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat
     7621 gttgttttgc agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat
     7681 caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc
     7741 tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc
     7801 cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct
     7861 gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt
     7921 gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga
     7981 cagataccca ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa
     8041 gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt
     8101 atttatccgg tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt
     8161 cagcatccgt tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgcc
     8221 ccctttcgta tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac
     8281 agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg
     8341 ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag
     8401 cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac
     8461 gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg
     8521 gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt
     8581 gtggcggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg
     8641 ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggta
     8701 ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg
     8761 ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagcttttt
     8821 tatcagtacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc
     8881 aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg
     8941 cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg aactgacact cctgctggtt
     9001 tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca
     9061 cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac
     9121 tcccgctaag ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg
     9181 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
     9241 gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga
     9301 gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc
     9361 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
     9421 gcatgatgaa atcagtcagt tcaccgatca gatggtgatg aatctggcgg actgggaaac
     9481 tcttccctac gacagctttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
     9541 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
     9601 gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg
     9661 tctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca
     9721 ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
     9781 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
     9841 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
     9901 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
     9961 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    10021 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    10081 tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt
    10141 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    10201 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    10261 ccgggtgcag ctaaaaacta aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    10321 ccagaaactg ccagacctgg ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg
    10381 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    10441 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    10501 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    10561 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    10621 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    10681 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    10741 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    10801 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    10861 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    10921 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    10981 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    11041 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    11101 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    11161 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    11221 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    11281 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    11341 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    11401 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    11461 ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    11521 tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    11581 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    11641 cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct
    11701 ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga
    11761 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    11821 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    11881 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    11941 gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggctgct
    12001 gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    12061 gctggaagat ctcggtgcag gctttgcgct ggcaacgcac gatctggaga tccgcggcgc
    12121 gggtgaactg cttggcgaag aacaaagtgg ctcaatggaa accatcggtt tctcgctgta
    12181 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    12241 agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga
    12301 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    12361 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga
    12421 tccggcgcgt accctgctgg atgttgcccg tctgcgccag caagcgcaga aactggggat
    12481 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    12541 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    12601 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    12661 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    12721 ttctgcgccc ccatcggcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    12781 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
    12841 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    12901 gccgtggcgc tggcgctacc ggcaaaagcc aacacttggc cgctgccgcc agcgggcagt
    12961 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    13021 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
    13081 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    13141 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    13201 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    13261 acaccgacaa tggtgaccac cgtttcagac aaacggcaaa cccaacctgg acgccaacgg
    13321 caaacacccg cgcccgttat aaagcacagg gaattgagtt gcctgcggta gtgccggctg
    13381 gaccggataa cccaatgggc catcatgcga ttcgtctggc ggcctatggc ggcgtttatt
    13441 tgcttcatgg tacgaacgcc gatttcggca ttggcatgcg ggtaagttct ggctgtattc
    13501 gtctgcggga tgacgatatc aaaacactct ttagccaggt caccccaggc accaaagtga
    13561 atatcatcaa cactccgata aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag
    13621 tacatcagcc gctgtcagag aagattgatg acgatccgca gctgctgcca attacgctga
    13681 atagcgcaat gcaatcattt aaagatgcag cacaaactga cgctgaagtg atgcaacatg
    13741 tgatggatgt ccgttccggg atgccggtgg atgtccgccg tcatcaagtg agcccacaaa
    13801 ctctgtaaat cctggataaa aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca
    13861 gcagtagcag gcattaatga gggttaatgc ttatttataa attactgctg ttccatggag
    13921 ggtattcgga ccggttacag aagtaatacg gaaagatttt gcgcccatct catccgcttt
    13981 ttgcgccagc tgctcttcca gcgatcccag atttgtcccc gctttagcac tgattgtacc
    14041 gactttttgt tggccttctg gcgttgactg aacttcgaca gccgcaaagc tggcaaatga
    14101 catggagctt aaaatcgccg cagcgatgag gatttttacg tttttcataa tagtggcctt
    14161 atgcagatga atgacgtcgc aagcaattac ttaacgatcg ataactaaat gacatatgtg
    14221 atctggatca catacaagat attttttata acaatcatta attaatttaa aaatctttaa
    14281 atttcagtta aatagaaagc aattattttt atgggatctg gactggtgaa cggaggtgat
    14341 tatttttata ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac
    14401 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
    14461 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
    14521 ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
    14581 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
    14641 cagctgtttt gtgaagagaa aagcgtgaag tctgcgctgg ctgactattt tgctgccatc
    14701 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
    14761 accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa
    14821 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
    14881 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
    14941 gcacgcgaag gtgcatcgct ggaaaaacta atgcagattg ccggaacgac tttgcgttta
    15001 tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc
    15061 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccattactat
    15121 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
    15181 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
    15241 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
    15301 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccgcga ccaccgccaa
    15361 actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt
    15421 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
    15481 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccacgttcaa
    15541 acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac
    15601 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
    15661 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata
    15721 cttaatgatt cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag
    15781 gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg
    15841 tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc
    15901 atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag
    15961 atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caagttaccc
    16021 atcaggctac caacggtgga gaagttcgac agcgatacca gtgaaccatg atctttatac
    16081 tgataatttt tcagcggctt accgtttatc tgcgccagaa tattgttcat tgcgcaagtc
    16141 gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgtgcat
    16201 gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc
    16261 accaccagct ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc
    16321 gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcacc atctttagtg
    16381 tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt
    16441 ttcgttaact cactatgggc cgcagcagag atgcgtggcg gtaatgcagg caaaatacgt
    16501 tctcccgctt ctactagcgt tacgttcagg gcttcgttgg tcagaccttt ataaccgtag
    16561 ctgtgcagtt gcttgaccgc gttatgcaac tcagcggaga gttctacacc cgtcgcgccg
    16621 ccaccgacaa tcgcaatgtt cactttgcca ttcgcgccca ggttggcgga gtatttcagg
    16681 aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg
    16741 cagttctctt tgacgcctgg cgtattgaaa tcgttagagg tgctacccag cgccatgacc
    16801 agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc gtcgcgtagt
    16861 tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc caactggaac
    16921 tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc
    16981 gagccagtcg ccacttcgtg cagcagcggt ttccacaggt ggctgtggtt acgatcgacc
    17041 agcgtaattt tggctttttt cttacgtccc agcttatgcc ccagctgtgt tgccatttcc
    17101 agcccaccag caccgccgcc gacaatcaca atctttttca atggcgtagt caacgtgacc
    17161 cccttaaatt tattaaccaa ttgttaatta atggttatta acatagctct taattaacaa
    17221 caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc
    17281 ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact ttaccgggtg
    17341 agatcaaaaa atagagttta ttgtttatta tttgagcgaa tttgtgagag tgatatagag
    17401 tgaggggtgt atgcagggcg ttcggtaggc ctgataagag gcgcatcagg cattgatgct
    17461 cgactgtcgg atgcggcgta aacgccttat ccgacttaca gaattcggag cgcatcaggt
    17521 accggcaagg ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg
    17581 cgctgcaaat gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc
    17641 tcgtaataat gatgcaactc ttcagatgtc cgctggctgt taagcgcttc atcattacgc
    17701 gacaaaatca ccaggcaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg
    17761 gcaatatcgg catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac
    17821 aaattagggt tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag
    17881 tatccgccca gaccaacgcc gcaaatcagc ggacgctcgt cgacgttcag ttgcaacatt
    17941 ttgtccactt ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt
    18001 atcaagcgta catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga
    18061 ctgttagagt caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta
    18121 gtgacctgct ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgctcgc
    18181 cttccagcga gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg
    18241 tgtaacacgc tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg
    18301 attattgcag accaggatca tatcgcaacc tgcgtccagt gatgcctgcc cgcgttcggc
    18361 ataactgccc ataatcgcgg caccttccat cgataaatcg tcagagaaaa ttacaccgtc
    18421 aaaacacagt tcctgacgca aaacggtttt cagccagtag gaagaaccgc tcgccggacg
    18481 cggatcaaca tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg
    18541 gattaacgtg ctgaaaaccg acatatcttt ggcacgaatt tccgcttgcg ggcgtgggtc
    18601 gcacggcgtc tctttgtgtg aatctgcagt tactgcaccg tgtcctggga agtgtttccc
    18661 ggtcgttttc attccggctt catgcatccc atcaataaac cgactggcga ttgctagggc
    18721 tttttctggg tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gtcctacatc
    18781 cagcactggc gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca
    18841 acccgcctct tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg
    18901 tgctgccggt aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc
    18961 taccgccacc accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa
    19021 ctgggcagga tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc
    19081 cagtatttca cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc
    19141 cacactgctc tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga
    19201 taaattgttg atcgccggtt tgtcgccagc ggtactcaaa ccaccctgct ttgagcatca
    19261 gcagccaggg aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc
    19321 gagtggcata gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg
    19381 ccagttccag cgcggtatca ccatctccgg catactccca gtcgatgagt tttaaccctg
    19441 atgcgctatg cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag
    19501 gtcgtggttc ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc
    19561 gcgccggatc gctttgctgc cagtacagtt ccagtaacgg caacagcgtt attcgccagc
    19621 caaaacgtgg ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca
    19681 aatacgtttt taccgcgccg ggcagatagt cgactaccat ccagtcacgg agatataaat
    19741 gcggcttcgg tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg
    19801 cggactgcgg cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt
    19861 caatgagaaa actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg
    19921 ggaaaaagcg cgacagcaat tcgtcgcgcg tgatgggatt attactgcga aacggcacct
    19981 ttacctgacc agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga
    20041 gcgttaacgt tgccagaggc gctggagtac agcacgtaat gagcgccgac attgcgggca
    20101 atgcctatgg ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc
    20161 ttagccatcg acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca
    20221 tttcgcagcg tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg
    20281 ctatcaacca gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc
    20341 atcggctgca ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt
    20401 cgatcttcgt gctcaattgg gcctggttgc tgcgggatcg tcggcaccga gggcactgtg
    20461 gggacaggct gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt
    20521 gcaggttcac gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg
    20581 tagcgactca tttttgtcat caagattcac cccttacaaa tatagataaa gtctgacttt
    20641 gtgcgccccc agaaaattgg cgctgccgta cagcgttacc gccgaatgtg cgggaatggt
    20701 aacgctgcgt ggcctttcca gaggatgcat ctccagccct ctggcgtcat accagtaaaa
    20761 acgataatga acggtaacgg gtttttgcct ttcgttataa agcgttgagg atgctgaagg
    20821 ttgaatatca gacgtcgaaa ggacgggctt ttctgcactg atgcccgcag ccaataaaga
    20881 tgactccatc accagcgatt gctcatcatt caccggaatt tccggatgtg aacgacagcc
    20941 caccagcagt aacaacacca gagacaccag cccaaagcat ccttttctca tcgttacaga
    21001 cctttatgcg ccagcattgg tcccagcgga cggccaccca acaagtgcat atggatgtgg
    21061 taaacctctt gtccgccatg gcggttggta ttcatgatca gacgatagcc atcttcggca
    21121 ataccttctt gctcagcaat ttttgccgct acggtgatca tgcgtcccag cgcctgctca
    21181 tgctcagctg agacgtcgtt cacagtcgga atgaggatat tcggaatgat cagaatatgc
    21241 gttggcgctt gcggcgaaat atcgcgaaac gccgttacca gatcatcctg gtagacgata
    21301 tcggagggga tctcacgacg aataattttg ctgaatatag tttcttctgc cacgacattt
    21361 tcctttttca aatagccatg cgttgcgcca tgctacgccg cgccacactg cgagtataaa
    21421 gtatggtcga gttaaacctg ctctttcaac cttaactcac gatttcttaa gcaaaaaatc
    21481 gttttgctgc cgtatatatc gccattattc ccatttctgc ctgcgaatgc gctgaaaaga
    21541 cattctctgt ttggtttaca cgacaaatat gaatgcgtat atttctcatt tgcatttaca
    21601 aacaaaatta ttcgcacact aaatataatc gctcatcacc ctcctccggc atgagcctga
    21661 cgacaacata aaccatgaga tttcaaatgc tttcaacaca atttaacagg gataatcaat
    21721 atcaagccat caccaaaccg tcactacttg ccggttgcat agcactggca ctattacctt
    21781 ctgccgcttt tgctgcacca gccactgaag aaacggtgat tgttgagggt tcagccacag
    21841 ctccagatga tggcgaaaat gattacagcg taacgtctac ctctgcgggt accaaaatgc
    21901 agatgactca acgtgatatt cctcagtcgg tcactattgt tagccagcag cggatggaag
    21961 atcagcagtt acaaacgctg ggcgaagtga tggaaaacac gctggggatc agcaaaagtc
    22021 aggcggattc cgatcgtgct ctttattatt cccgcggatt ccagatcgat aactatatgg
    22081 ttgatggtat ccccacctat tttgaatcgc gctggaatct gggcgatgca ctttctgata
    22141 tggcactgtt tgaacgcgta gaagtagtgc gtggcgcgac aggactcatg accgggacgg
    22201 gtaatccatc tgcggcaatt gatatggttc gaaaacacgc gaccagtcgt gaatttaaag
    22261 gcgatgtctc ggcggaatac ggtagctgga acaaagaacg gtatgtggcg gatttacaaa
    22321 gcccactcac cgaagacggt aaaatccgcg cgcgaattgt cggcggctac cagaataacg
    22381 actcatggct ggaccgctac aacagtgaaa agaccttctt ctccggcatt gtcgatgctg
    22441 atttaggcga tcttacgacg ctttcagccg gttacgaata tcagcgcatt gatgttaata
    22501 gccctacctg gggcggttta ccgcgctgga atactgatgg cagcagcaac agttacgatc
    22561 gtgcacgcag taccgcacct gactgggcgt acaacgataa agagatcaac aaggtcttta
    22621 tgaccctgaa gcagcggttt gctgatacct ggcaagcgac actgaatgcc acccactctg
    22681 aagtcgaatt tgacagcaaa atgatgtatg tcgatgccta tgtaaacaaa gcggatggta
    22741 tgctggttgg gccatacagt aattgtggac ctggctttga ttatgtcggc ggcaccggtt
    22801 ggaacagtgg caaacgtaaa gttgatgcgc tggatttgtt cgctgacggt agttatgaat
    22861 tgtttggtcg tcagcacaat ctaatgtttg gtggcagtta cagcaaacaa aacaatcgtt
    22921 acttcagttc atgggccaac atcttcccgg atgaaattgg cagtttctac aactttaatg
    22981 gcaatttccc acaaaccgac tggtcaccac agagcctggc acaggacgat accacacata
    23041 tgaaatcgtt atatgctgcc actcgagtca cccttgccga tccgctgcat ctgatcctcg
    23101 gcgcacgtta taccaactgg cgggttgata cgctgactta cagcatggag aaaaaccaca
    23161 ccacgcctta cgctggtctg gtgtttgaca tcaatgacaa ctggtcgacc tacgccagct
    23221 atacctctat tttccagccg caaaatgatc gtgacagttc aggcaaatat ctggctccaa
    23281 tcaccggtaa caactacgag ctgggtctga aatcggactg gatgaatagc cgtctgacca
    23341 ccacgttagc catcttccgt attgagcagg ataatgtcgc tcagtccacc ggtacaccta
    23401 tccccggcag caacggcgca attaccgaca actggcagct gacatttggc gcaacgcgct
    23461 atattgcaga ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc accacggtta
    23521 aaatgttcac cagctatcgg ttgcctgtca tgcagagttg acagtcggcg gtggtgttaa
    23581 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
    23641 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
    23701 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacatgg aaggttctat
    23761 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
    23821 tgtaaaaaag gcagccatct ggctgccttt gtctccccaa cgtcttacgg attagtggtt
    23881 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
    23941 aacaccagaa cccgcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
    24001 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
    24061 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
    24121 tgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
    24181 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcgggaacag
    24241 ccacagtttg ctgctgttac cagacagaac gataaagtcg atcagaccgt gcgagaacga
    24301 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggccta ccagaatcgc
    24361 gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat
    24421 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt
    24481 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    24541 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    24601 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    24661 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    24721 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    24781 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    24841 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    24901 gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga gtacgccagt
    24961 atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    25021 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    25081 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    25141 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    25201 cgcggaaccg acgcccagca gaatacctgc gataggtagt acggataccg gcagcatcag
    25261 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    25321 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    25381 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    25441 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    25501 atatgtcaca atatttcaaa cgcggttatt caccgcccaa ttttcggcgg tgaactttac
    25561 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    25621 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    25681 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    25741 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    25801 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
    25861 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    25921 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    25981 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    26041 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    26101 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    26161 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    26221 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
    26281 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    26341 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    26401 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    26461 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtgacagac attaactgtt
    26521 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    26581 ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgtgcagcg ccatgatggc
    26641 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    26701 gattaagggc cgccagcagc gaataccagt cgcccgatgg tacgctatat gccaacgcct
    26761 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    26821 gcgaaccggc acttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    26881 gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt aaccgacaac
    26941 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    27001 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    27061 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    27121 cggtgacctc acgtaccgca tcaatgccca gcgtattttt ccctttttcg ggagccaggg
    27181 tgtagtaatc gggatgcgtg ccagcctgca ttaactgaca tccacgacag tgaccgcaac
    27241 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    27301 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    27361 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    27421 ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    27481 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    27541 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    27601 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa
    27661 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    27721 gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg tgccgttagc
    27781 cagagctggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    27841 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    27901 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    27961 cagttgctca agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    28021 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    28081 gcacagactt gttatggctg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    28141 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    28201 ccggcgtagc tatcgcacct ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    28261 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    28321 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    28381 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    28441 gctggttttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    28501 ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta ttggcggtat
    28561 acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtt
    28621 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    28681 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    28741 cttctttacc gctttccagc aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    28801 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcaac cattgaaaaa
    28861 cccgtgggcg attgatgatc ttatcggcat aaagctgttc accaagcgcc agacgtccgg
    28921 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    28981 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    29041 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    29101 atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat
    29161 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag aggcttgcac
    29221 ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc
    29281 gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc gccagaacaa
    29341 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    29401 gtttgtctgc tcaagatgag agcggatcaa tacttgctca agccgattaa gatgtttaat
    29461 acctgcaaga tggggattgc gccccagccg caccgggctt agcgccaacg tcatcccttc
    29521 gttacgcaaa cggtcgtaat gtgcaggata aaccgtaacg gagagaatcc gcgttgccgg
    29581 tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    29641 cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gaggccagaa
    29701 gtcacagaaa atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa
    29761 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    29821 tgcccgatcg cttactgcca gcgattcctg cttatgaccg ttaattaaga acataccggc
    29881 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    29941 gctaagaaaa aaggcccgca agcggacctt ttatatgggt gggaaatgac aacttagatc
    30001 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    30061 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    30121 aggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    30181 atagactcta ctgcccccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    30241 accaacacac ggcttgcagc ttcaccgaag atggctttca ccgcctgcgc ttcagcttta
    30301 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    30361 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    30421 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    30481 gcaccgcgtt ttttcgcgtg ttcgtactct tctagtacca gcataccggc accatcgccc
    30541 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    30601 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    30661 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatga
    30721 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    30781 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    30841 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    30901 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    30961 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    31021 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    31081 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    31141 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    31201 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa
    31261 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    31321 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    31381 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    31441 gtgtcaagag aatccgcgcc taggtcttca acgaaagaag cattgttggt aacttcttcc
    31501 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    31561 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    31621 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    31681 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    31741 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    31801 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    31861 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    31921 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    31981 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    32041 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    32101 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    32161 aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt cattcgcatt
    32221 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    32281 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    32341 cctttgccgt tagcacctaa ataatcactg atcgcctgag cgccattttc actggtcgca
    32401 gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    32461 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    32521 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    32581 acaatgcgtt tcgtcaggcc agttagcact ttgcccgggc cgacttcata gagatgttct
    32641 acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga
    32701 cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    32761 acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    32821 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt
    32881 tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcctcttt atgaccggca
    32941 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct
    33001 tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcgccc
    33061 gtgccttctg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca
    33121 tcagcgaaat caatcacgcc agcgcaaacc aacgcggagt attcccccag gctgtgaccg
    33181 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat
    33241 gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc tggcccctgc
    33301 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    33361 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    33421 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg
    33481 gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    33541 cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg
    33601 tggcgatcca gcgtcaccac gacattgtcc atagacatac cgagtttttt cgccgttgca
    33661 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    33721 ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag
    33781 acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt
    33841 ggcagcatca gcaattcacc ataactaccg tcggcatgca gatgggtgga gatgattccc
    33901 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    33961 ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    34021 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg
    34081 caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct ttgaatctga
    34141 caagctgcgc tcgggaaagc gtgcgtagca gacgtcgttg ccacaacgat caggccaatc
    34201 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    34261 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    34321 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    34381 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    34441 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    34501 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    34561 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    34621 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    34681 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    34741 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    34801 agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc ctgtaaagcc
    34861 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    34921 ataattgata gaagggattg ttttaagcac cgctgaggcc tcccgaatac tgtcgagacc
    34981 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac
    35041 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc tgtcacaatc
    35101 gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt
    35161 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    35221 cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    35281 cagggccata cgcattgaac tcccacgact agcgcggata gcttgcgaag gccgggcatc
    35341 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    35401 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    35461 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    35521 catgacatct aacgccaagg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    35581 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    35641 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tatggtgacg caggtgtttt
    35701 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    35761 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    35821 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    35881 aatacggcaa atgggtttgg cttttgcact tcttcaggca gttcaccaaa gaccatgtcc
    35941 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    36001 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    36061 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    36121 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    36181 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    36241 cattccacat cactgtctac actgactacg gattcggcga cgcgctcaac ctgatcaggg
    36301 gtatagatcc cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    36361 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    36421 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    36481 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    36541 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    36601 ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    36661 caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    36721 tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt
    36781 cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg
    36841 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    36901 tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc
    36961 gtcacctgag cgaggcggag attgataatt acgtgcgtaa ggagcatccc ctgcactgcg
    37021 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gagggacgtg
    37081 accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag
    37141 ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg catccggcaa
    37201 tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg
    37261 cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca
    37321 tccatcggcg cttcgatacg catcacctca ccggtccccg gatgggtaaa cttcaacgct
    37381 gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat
    37441 tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg tgtatgcaca
    37501 cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc aaatgcatag
    37561 cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac acgcacgata
    37621 cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt cacatgtgac
    37681 tgccactgac tgcgcaccag cgccaggtaa tctttttgca ttcctttttc acgtaattgc
    37741 tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc
    37801 cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct
    37861 tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc
    37921 agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc
    37981 agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc
    38041 tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttcg
    38101 cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa
    38161 aagttgtcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga
    38221 gtctctgttt tcatggtgag cgattctaat tagccaacag gattcgcgcc actcattttt
    38281 ctatgcttat atttagtttt acaccttatt acttcactac gtgatcactt tattgatggt
    38341 tattaaacca atcaccagca aga
//
LOCUS       DAJTMK010000018        37634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTMK010000018 DAJTMK010000000
VERSION     DAJTMK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37634
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.018"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..1987
                     /locus_tag="OO135_000781"
                     /pseudo
     CDS             1..1987
                     /locus_tag="OO135_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            2029..2706
                     /gene="ybiX"
                     /locus_tag="OO135_000782"
     CDS             2029..2706
                     /gene="ybiX"
                     /locus_tag="OO135_000782"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5396726.1"
                     /translation="MMYHIPGVLSPQEVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2780..3046
                     /gene="ybiI"
                     /locus_tag="OO135_000783"
     CDS             2780..3046
                     /gene="ybiI"
                     /locus_tag="OO135_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5396727.1"
                     /translation="MASGWANDEAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3311..3571
                     /locus_tag="OO135_000784"
     CDS             3311..3571
                     /locus_tag="OO135_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5396728.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3799..4884)
                     /gene="hcxB"
                     /locus_tag="OO135_000785"
     CDS             complement(3799..4884)
                     /gene="hcxB"
                     /locus_tag="OO135_000785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5396729.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5025..5987)
                     /gene="ybiB"
                     /locus_tag="OO135_000786"
     CDS             complement(5025..5987)
                     /gene="ybiB"
                     /locus_tag="OO135_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5396730.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6015..8165)
                     /gene="dinG"
                     /locus_tag="OO135_000787"
     CDS             complement(6015..8165)
                     /gene="dinG"
                     /locus_tag="OO135_000787"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5396731.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCAGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8285..8767
                     /gene="ybiA"
                     /locus_tag="OO135_000788"
     CDS             8285..8767
                     /gene="ybiA"
                     /locus_tag="OO135_000788"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5396732.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTR
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8999..10363)
                     /gene="rhlE"
                     /locus_tag="OO135_000789"
     CDS             complement(8999..10363)
                     /gene="rhlE"
                     /locus_tag="OO135_000789"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR5396733.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10592..11263
                     /gene="cecR"
                     /locus_tag="OO135_000790"
     CDS             10592..11263
                     /gene="cecR"
                     /locus_tag="OO135_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5396734.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     LQRSL"
     gene            11266..12261
                     /gene="hlyD"
                     /locus_tag="OO135_000791"
     CDS             11266..12261
                     /gene="hlyD"
                     /locus_tag="OO135_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5396735.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12254..13990
                     /locus_tag="OO135_000792"
     CDS             12254..13990
                     /locus_tag="OO135_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5396736.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13983..15116
                     /locus_tag="OO135_000793"
     CDS             13983..15116
                     /locus_tag="OO135_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5396737.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIHAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15127..16233
                     /locus_tag="OO135_000794"
     CDS             15127..16233
                     /locus_tag="OO135_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5396738.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16195..16605)
                     /locus_tag="OO135_000795"
     CDS             complement(16195..16605)
                     /locus_tag="OO135_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5396739.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16738..17499
                     /locus_tag="OO135_000796"
     CDS             16738..17499
                     /locus_tag="OO135_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5396740.1"
                     /translation="MPDQTQQFSFKVLTINIQKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17496..18737
                     /gene="clsB"
                     /locus_tag="OO135_000797"
     CDS             17496..18737
                     /gene="clsB"
                     /locus_tag="OO135_000797"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5396741.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18737..19693
                     /locus_tag="OO135_000798"
     CDS             18737..19693
                     /locus_tag="OO135_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5396742.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVA
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALTQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(19729..20442)
                     /locus_tag="OO135_000799"
     CDS             complement(19729..20442)
                     /locus_tag="OO135_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5396743.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVGIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20648..21352)
                     /locus_tag="OO135_000800"
     CDS             complement(20648..21352)
                     /locus_tag="OO135_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5396744.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21489..21941)
                     /gene="moaE"
                     /locus_tag="OO135_000801"
     CDS             complement(21489..21941)
                     /gene="moaE"
                     /locus_tag="OO135_000801"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5396745.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALSLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21943..22188)
                     /gene="moaD"
                     /locus_tag="OO135_000802"
     CDS             complement(21943..22188)
                     /gene="moaD"
                     /locus_tag="OO135_000802"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5396746.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22181..22666)
                     /gene="moaC"
                     /locus_tag="OO135_000803"
     CDS             complement(22181..22666)
                     /gene="moaC"
                     /locus_tag="OO135_000803"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5396747.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22669..23181)
                     /gene="moaB"
                     /locus_tag="OO135_000804"
     CDS             complement(22669..23181)
                     /gene="moaB"
                     /locus_tag="OO135_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5396748.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIALQLDARTC
                     PCNFHPHLKK"
     gene            complement(23203..24192)
                     /gene="moaA"
                     /locus_tag="OO135_000805"
     CDS             complement(23203..24192)
                     /gene="moaA"
                     /locus_tag="OO135_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5396749.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24180..24324)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24589..25497
                     /gene="yvcK"
                     /locus_tag="OO135_000806"
     CDS             24589..25497
                     /gene="yvcK"
                     /locus_tag="OO135_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5396750.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25689..27710)
                     /gene="uvrB"
                     /locus_tag="OO135_000807"
     CDS             complement(25689..27710)
                     /gene="uvrB"
                     /locus_tag="OO135_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5396751.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(28289..28966)
                     /gene="bioD"
                     /locus_tag="OO135_000808"
     CDS             complement(28289..28966)
                     /gene="bioD"
                     /locus_tag="OO135_000808"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5396752.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQYAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28959..29714)
                     /gene="bioC"
                     /locus_tag="OO135_000809"
     CDS             complement(28959..29714)
                     /gene="bioC"
                     /locus_tag="OO135_000809"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5396753.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29701..30855)
                     /gene="bioF"
                     /locus_tag="OO135_000810"
     CDS             complement(29701..30855)
                     /gene="bioF"
                     /locus_tag="OO135_000810"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5396754.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30852..31892)
                     /gene="bioB"
                     /locus_tag="OO135_000811"
     CDS             complement(30852..31892)
                     /gene="bioB"
                     /locus_tag="OO135_000811"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5396755.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVHGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31979..33268
                     /gene="bioA"
                     /locus_tag="OO135_000812"
     CDS             31979..33268
                     /gene="bioA"
                     /locus_tag="OO135_000812"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5396756.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGKVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            33327..33803
                     /locus_tag="OO135_000813"
     CDS             33327..33803
                     /locus_tag="OO135_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5396757.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33955..35238
                     /locus_tag="OO135_000814"
     CDS             33955..35238
                     /locus_tag="OO135_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5396758.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35379..37616)
                     /locus_tag="OO135_000815"
                     /pseudo
     CDS             complement(35379..37616)
                     /locus_tag="OO135_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
BASE COUNT         8819 a        10186 c         9740 g         8889 t
ORIGIN      
        1 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgtgg tgccgatacc tctaacagta tttatattga tggcattcgc gatatcggca
      121 gcgcctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccgtccggca
      181 ccgactacgg gcacagcgca ccgacgggct cgatcaatat gatcagcaaa cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacca ccgcggtgcg cctgaatgta atgggcgaaa
      361 aaacgcacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tcgcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcacccagc
      481 acaacacgcc agacggcggc attccgacca tcggtttgcc gggctattct gccccatctg
      541 cgggaacggc ggccctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
      601 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
      661 acgataacac caccattcgc aatactaccc gttggtcgcg cgtaaagcag gattacctga
      721 tgacggcgat tatgggcggg gcgtcgaata ttactcagcc caccagcgat gtgaatagct
      781 ggacctggtc acgcacgcga ataccaaaga tgtgagtaat aaaattctca ccaaccagac
      841 caacctgacc tcgacgttct ataccggttc tatcggtcat gatgtcagta ccggcgtgga
      901 atttacccgt gaaacgcaga ctaactacgg cgttaatccg gtgacgttac ccgcggtaaa
      961 tatttatcat cctgacagca gcattcatcc cggcggcctg acgcgcaacg gcgcaaacgc
     1021 caatggtcag acggatacct tcgcaattta cgcctttgat acgctgcaaa tcacccgtga
     1081 ttttgagctg aacggcggga tccgtctgga taattatcat actgaatatg acagtgccac
     1141 cgcctgcggc ggcagcggac gcggtgccat cacctgccca gctggtgtgg caaaaggttc
     1201 tccggtcacc accgtcgaca ccgccaagtc gggcaatctg gtgaactgga aagccggggc
     1261 gctgtatcac ctgacggaaa acggtaatgt ctatattaac tatgccgttt cccagcagcc
     1321 tccgggcggc aacaacttcg cccttgcgca gtctggcagc ggtaacagtg ccaaccgcac
     1381 cgattttaaa ccgcaaaaag ccaacaccag cgagattggc accaaatggc aggttctgga
     1441 taaacgcctg ttgctcaccg ccgcactgtt ccgtactgat atcgaaaatg aagttgagca
     1501 aaatgatgac ggaacttact cgcaatacgg taagaaacgt gtcgaaggct atgagatatc
     1561 cgtggccggg aatatcactc ccgcgtggca ggtgattggc ggctataccc agcaaaaagc
     1621 aaccatcaaa aacggcaaag atgttgccca ggatggttcc tcatcgctgc cgtatacccc
     1681 ggagcacgcc ttcaccttat ggagccagta tcaggcaacc gatgatatct ctgttggcgc
     1741 gggcgcacgc tatatcggca gtatgcataa aggttcagac ggcgcggtgg gaacgccagc
     1801 gtttaccgaa ggttactggg tcgccgatgc caaactgggg tatcgggtta atcgcaatct
     1861 cgactttcag ctaaacgttt acaacctgtt tgataccgat tacgtcgcct caatcaacaa
     1921 gagcggctac cgttatcacc cgggcgagcc aagaaccttc ttgctcacag ccaatatgca
     1981 tttctgattc agatgtgggg cgcaggcccc actttttgga gaaattgtat gatgtaccac
     2041 attcccggcg tgttatcgcc acaggaggtc gctcgttttc gcgaacaact ggaacaagcc
     2101 gaatgggtgg atggacgcgt caccaccggc gcacaaggtg cgcaagttaa gaacaatcaa
     2161 caggtagaca cccgcagcac gttatacgcc gccctgcaaa atgaggtgct gaacgcggtt
     2221 aaccaacatg ctttattctt tgccgcggcc ttgccgcgta ccctttccac gccgctgttt
     2281 aatcgctatc agaacaatga aacctatggt tttcatgtgg atggcgcagt acgcagccat
     2341 ccacaaaacg gctggatgcg cactgacctt tctgccacgc tgtttttaag cgatccacaa
     2401 agctacgacg gcggcgaact ggtcgttaat gacaccttcg gacaacatcg ggtaaaactc
     2461 ccggcaggtg atctcgtgtt gtatccctcc agcagcctgc attgcgtgac acccgtaacc
     2521 cgcggcgtac gagtggcatc atttatgtgg atccagtcga tgatccgcga tgataaaaag
     2581 cgcgccatgc tgtttgaact agacaaaaat attcagtcgc tgaaaagccg ctatggtgaa
     2641 aatgaagaga tcctgtcgct gctcaatctt tatcataatc tgctgcggga atggtcggag
     2701 atctgacgct tgaactgccc aggttcgtcc ctatattagc tacacttaac tgtacaagca
     2761 ttgatatggg gaggacgata tggcatccgg ttgggctaac gatgaagccg tcaacgaaca
     2821 gatcaacagt acaattgaag atgcgattgc ccgcgctcgg ggtgaaattc cgcgtggcga
     2881 aagcctgtat gaatgtgaag agtgcggtgc ccccatcccg caagcccgtc gggaagccat
     2941 tcctggcgtg cgcttatgta ttcattgtca gcaggagaaa gatttacaaa aaccagcgta
     3001 tacaggatat aatcgcagag gttcgaaaga cagccagtta cgttaactac aactggcgag
     3061 atgcatagcg agtataattt gtattttgcg tggcattccg tgcctttaac gctgctgtga
     3121 ctgccacgcg gcataaaaca agtccataag tacaacaaat aaatggttta tcagtatgtt
     3181 agatatcaat caatttattt gaacaaggcg gtcaattctc ttcgatttta tctctcgaaa
     3241 aaaaacgtga tactcatcac atcgacgaaa caacgtcact tatacaaaaa tcacctgcga
     3301 gagattaatt atgaaaacta tcaatactgt tgttgctgct atggctcttt caactctatc
     3361 atttggcgtg ttcgcggcgg aaccggtaac ggcatcccag gcacagaaca tgaataaaat
     3421 cggcgtggtt tctgccgatg gcgcatccac cctcgatgcc ctggaagcga aactggctga
     3481 gaaagccgcg gcagccggtg ccagtggata cagcatcact tctgccacca acaacaataa
     3541 attaagcggt actgcggtaa tttataagta attcgtagga cggataaggc gtttacgccg
     3601 catccggcaa cagatgcctg atgcgacgct accgcgtctt atcaggccta caaaatccga
     3661 accgttggac ggatcaggcg gttacgctac gtccagcaca agtatttccc tgtctgtttg
     3721 ccgacagacg catatgctct aaccctcatt gatcctatat tacccttgtt tgcccgtccg
     3781 ttactggacg ggcttttttt agctggctaa ctgctgacag aaagcctgca acgtctcttc
     3841 cgacatacca atctgccgcg ccgcatcaca aatggcctgc cagcttcccg catccaatgg
     3901 aatcccctgc tcctgccgtt cgcgacgcgt gttcacttcc cactcgcccg gtagcaaaat
     3961 cggcttatcg tcatcatgcg gcgaggcttt cacccactcg gcaaaggctt cagtctgcgc
     4021 gctacaatcc ggcgcgccga acagttccgg gttgatgatg atagtggtca tgcagttaag
     4081 aatggcatcg ggactggttt gtaacgtttc ctgatgcgtc gttttaccgc cagaaagcgc
     4141 cccgccaaga atttcacaca tcgctgcaag ggcgtagcct ttatgttcgg caaaggtcaa
     4201 cagcgacccc aacggcgact cctgcattac cgccggattg gtcgtcggca cgccgttaac
     4261 gtcaatcagg caacctggcg gcacggggac tcctttatgc caggcgacgc gagttttacc
     4321 aaatgcgatg gcgctggtgg cgtaatcaag caacagcggg aaattatctt tacgagggaa
     4381 aaccacacag aacggattgg tgccaaagcg gctgtcgcga ccgtggaacg gtgcgaccat
     4441 tggaataccg accacgctaa caaagtggat agagacaaac cccgccgctg cacactgctc
     4501 cgcccagtaa ccgatacggc cgatatgatg cgagttatgt agcgccacgg cagcaatacc
     4561 gtgctgatgc gctttctcaa tccccagcgc catcgcttcg tgtgccgcga cctgaccgaa
     4621 tgcgcgatcg ccatcgagcg tgactgccgc ccccgcctct ttaacgattt tggcatgatg
     4681 gttaatttgc agatgcccct gactccagga acgcacatag cttgggatca tgccaatacc
     4741 atgagaatca tgccctgcca ggtttgccgc gattaaatga tcggccacta atttcgcttc
     4801 ttgttcctcg ctacccatct gacgaaatac agcctgaata aaactgtgca gcgtctgagc
     4861 atcaaagcga tgaccacttt ccatggttaa ctcctgttta tgttatgtgt ttgttgtttt
     4921 tttatgctgc acgccgggcg cgggaaggtc aataaagatg caggaatttc atattgcggt
     4981 ggggtaaaag ccggatgaca tggctcatcc ggctgagaaa agaattaaaa tgcctgatta
     5041 acgcgcgcca ggccgtcact gatagttgcc gcttcacccg tagcctccag gcagcaagcc
     5101 atctggattt tcagcgattc gggaatcggt tcgctgccag caaggcaacg ctcaatccac
     5161 tgcgccgtgg tttctgggtc ttttgcttgt ggcagtaact cgctaccagc agtgtcctgt
     5221 ttttcataca gcacccgcat cccttcacgg tcaatgagat tgatctgcgg gcagcgctgc
     5281 ggattagcat acacttcgcc ttcagtacca tgcattagta gcgcccgccc gccgatatcg
     5341 ctaaagaact tcgcgactcg cccgatgtat tccggatgcg aaacgctgga aagacgcagc
     5401 gcctcacctt cggcaaatgg cgtcgccagt ttcgccaggg tatgtgcact gttacgcacg
     5461 cccatccgcc agcgcatcgc cagttgtttt tccagcggcg ggcaaaacgc gccgacaggc
     5521 ataaacaccg gttgatgttc gtcgagcttc gcctgcgcct gcccgccgtg caacgttggt
     5581 gtaataccca tcagttcgaa aatggtttct gtcagcacgc gagttggatc ttcgctaacc
     5641 ccgtgaacca ccacaggaaa accgagtttg tgcaggagaa tagccaacaa cggcgtcagg
     5701 ttggcctgtt tacgcgcgcc gttgtaactg gggatgacaa tcggcatcgg cttgcctgca
     5761 ggcggcgtca gcttgatggt gtgattttgc atggcttcgt aaaagccgag catctctgct
     5821 tccccttccc ctttgatacg tagcgcaatc aatacgccgc ccaactcaag gtcagggact
     5881 tcaccattga gcatatgggc atacagaccg cgcgcagtat cccggtctaa atcgcgcgcg
     5941 tggtttttcc cgcgcccgat ctctttaatg attttgcgat agtccattta cgactcctta
     6001 cctgactcac atcattaacg ccgccgacgg cgtggggatt tcgttttttc tttcttttta
     6061 actatacctt cagggacttc cggttgctct atcggaaata ccggtaatgc atccagtagt
     6121 cgcttgccat aatttttggt cagcaagcgt ttgtcgtaga taaccacttc gccccagcaa
     6181 ccgtggcttc gaatcagtcg cccaacctgc tgaatcaggt taaacgaggc gctcggcagg
     6241 ctttgcacct caaacggata gcggttgagg cttttcagcc attccccttc ggtgatcacc
     6301 accgggctgt cgatgggcgg aaaagcgatt ttgtggatat gcacctggct gagcagatca
     6361 cctttcaaat caagcccttc ggcaaatgac tgtaagccca ccagcacact acgctcaccg
     6421 ttggcaacgc gtttgcggtg cagttcaact aagcgataac gcggctgatc gccctgcacc
     6481 agcagcatca ggcgtaaatc cgtcacatag tcgagaaagc gctgcatcgc ccgtccgctg
     6541 gcaaacagta ccaacatacc gagatgtttt ttgctctcca cctgctcacg gaaaaaggcc
     6601 gccatttcgg caatatgctg ctcttcgttg tcgatggaag gctcaaagcg catccgggga
     6661 ataacaattt tgccctgttc gcagtggtta aagggggaat ccagcgccac aaaacggtcg
     6721 cccgctttct ctttcagccc gctcatctcc tgcaaacgcg aaaaactgtt cagcgaacgc
     6781 aaggttgcgg aggtgacaat aatgtgcgga atactgcgcc acagcagcct ttccagctga
     6841 tcgctgacac gtattcccgc gcagtgaaac cagagatgca gctgcccttc gcgctcttcc
     6901 cgcgtcgccc atttggtcac cggcgcaccg gaagattgcg ccagagacgc cagccgccag
     6961 agtttgcttt gcgcctcgaa catccccaac gcgcggttca tctgcaaaat caaccgatgc
     7021 agacgcacaa tatcgtggct gccggttttc tcacttaaat cgttaagaaa taactccgcc
     7081 agaccacgca gcatctcggt gagttttgcc agccgctggc agatctccag cacttcatca
     7141 ggcagttcgc ccatcgcaaa acggtgctct gcctcctgcc cggcaggcat gtagagattg
     7201 agaatgttgt ttaacgaggc gataagctca tacaactctt cacaatgcgc attcaaacgt
     7261 tcagggatcg ccagcggtgg gatggtcttc gggcgaaact gctccatgca ggtagcgacc
     7321 agtttcgtga acaagtccag ctgtagtcga taccagggcg cggtgatttc agcgctcatc
     7381 tccagcgcat cccgggccac atctggcagg tgatgacctt cgtccagcac cagcagcaga
     7441 tttttcgggt caggcagtac ggcttcgctt tccatcgccg ccatcaccag cgcatggttt
     7501 gccaccacca cttccgcttc ctgaatctcc cgacgagcaa caaaaaacgg gcattcacga
     7561 tagtagtaac agttacggtt gaggcagctg gctttgtcgg tactcaaacg acgccagaga
     7621 tcatcatcta tagcgatatc cgtatgatcg cgcagaccat cccatttata agtgtcgaga
     7681 tcgcccttca gcttcgcaca acgtttttgc tcttcctgat tatttggcgt cagttcgtcg
     7741 tcaagaaacg ccagcagatc ctgttgcgtg ggttcagtgc tggcgagcgc cgtcagatta
     7801 cgcggacaaa cgtagcgccc acgcccgaaa gcggcagtga atttaagatc gggaatgatc
     7861 tttttcagca gcggtaaatc tttgctgtaa atctgatcct gcaacgccac gttggcggta
     7921 ctcaccacca gcgttttttg ctcttcgcgg gcaatggcga tgccggggat caaataggag
     7981 agcgttttcc caacgccggt gggggcttca atcgccagat gccgcccttc ttctccggcc
     8041 agcgttttgg cgacgtccgc aatcatctgc cgctgcggcg cacggggaat aaagtcgggg
     8101 atctgttcct gaagcgcctt ataccaggcg gcaatttgcg ctttaagcgc ggcggttaat
     8161 gccatgagaa aacctgaaat actgtataaa cagccaatat tgtggcattt ttcggttttc
     8221 aggggtaatg gtttttctgt ttctggaaag cggctcgcaa taaccaccaa cataatgcgg
     8281 tttaatgccc gttcgagcac aaaggatcca acacgtcatg caagacacca tcataaattt
     8341 ctacagcacc agcgatgact acggtgattt ttctaacttt gccgcctggc ccatcaaggt
     8401 tgacgggaaa accaggccca cctccgaaca ctattttcag gcacaaaaat tccttgatga
     8461 aaaataccgc gaagagatcc gtcgggtttc ttcccccatg gtcgccgctc gcatggggcg
     8521 taatcgttcc aagccacttc gtaaaaactg ggagtcggtt aaagaacagg tgatgcgtaa
     8581 agcacttcgt gctaaattcg aacagcatgc agaactgcgt gcgctcttgc tggcaaccgc
     8641 gcccgcaaaa ctggttgagc atacggaaaa cgatgcttac tggggagacg gtggtaatgg
     8701 taagggcaag aatcgactgg gctacctttt aatggagttg cgcgaacaat tggctataga
     8761 gaagtaacgt ctcggtggct tatgggcggt aaaacagata aattttttcc cgtgttctca
     8821 aattgctgac aagtgcttgt tgtgaaatgc cggatgcggc gtaaacgcct tatccggcct
     8881 acaaaagcat gcaaattcaa tatattgcag agttaacgta ggcctgataa gcgtagcgca
     8941 tcaggcagtt ttgcgtttgt tcatcagcct gatgccgggc atagcccggc ataaaagatt
     9001 actgcgcagc ggcaggttta cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg
     9061 catcgccgag gcggcgagac ggtttttctg caggtttcgc gcttgcagat tttgcgccac
     9121 cttccccacg gcgtggttgc tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc
     9181 cacgttgctg gcgaccgttc tggatcggtt cggctttgat tgacgggtcc ggctcatagc
     9241 ccggaatcgc aatgcgcggg atctcttttt tcagcagttt ttcgatatca cgcagcagtt
     9301 tgtgttcatc aacacacacc agcgacaacg cttcaccggt agcagccgca cgaccggtac
     9361 gcccgatacg gtggacataa tcttccggta cgtttggcag ttcatagttg acgacgtgcg
     9421 gcaactcttc gatatccaga ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt
     9481 cactcgattt aaaatcagcc agcgcacggg tgcgcgcacc ttgcgattta ttcccgtgga
     9541 tcgccgcact acggatgcca tctttattga gctgctcagc cagatggtta gcgccgtgtt
     9601 tggtacgggt aaacaccagc acctgctgcc agttcccttt accaatcatg tgcgacagca
     9661 attcgcgttt gcgtttctta tcgacaaagt gaacgtgctg ggtcacctga tcagacgcgg
     9721 tattgcggcg tgccacttcg atttccagcg ggttgtgcaa cagtttttcc gccagggctt
     9781 taatatcgtc agagaaggtc gcagagaaca gcaggttctg gcgtttcgct ggcagttttg
     9841 ttaacacgcg acggatatcg tggataaagc ccatgtcgag catgcggtca gcttcatcga
     9901 ggacgaggat ttcaacctga tccagcttca ctgcattttg atgttccagg tccagcaaac
     9961 gtcccggtgt tgccaccaga acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa
    10021 tgctgacacc gccaaacacc accagcgaac gaatgttcaa atatttgctg taatcacgga
    10081 cgttttcgcc aatctgcgca gccagttcac gggtcggggt aagaatgagc gcacgtaccg
    10141 gacgacgccc tttggcgtgc ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa
    10201 agcccgctgt tttaccggta ccggtctggg cgctagccat caggtcgcgg ccttccagca
    10261 ccgcagggat cgcctgctgc tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg
    10321 cgcgcaggat atcagggctt aaacccaaag aatcgaaaga cataactact ccgaaccgcc
    10381 ccgaccgtta ccggtgtagt tttcagggag atactgaaaa ggaaaatgac aaaaaccaca
    10441 atgtcatgag ggagcggagt gtagcagttt ttgtgacgca gcgcataaat tatccctgtg
    10501 actggcgttg ctaaaattct tagccatact ggacaactcc cctatggagt cataatctta
    10561 atcaatcatt tgattaagaa tggactgtgc gatgaataat cctgccatga caatcaaggg
    10621 tgaacaggcg aaaaaacagc tgattgctgc cgcactggcg cagtttggtg aatatggaat
    10681 gaacaccacc actcgcgaga tagccgccca ggccgggcag aatatcgctg ccatcaccta
    10741 ctacttcggt tcgaaagaag atttgtacct cgcctgcgcc cagtggattg ccgattttat
    10801 tggcgagcag ttccgtccgc atgccgagga agccgaacgc ttgttcgcgc aaccacagcc
    10861 tgatcgggct gccatccgtg aactgatcct tcgcgcctgc aggaacatga ttaagctgct
    10921 cacccaggat gacaccgtca acctcagcaa gtttatctcc cgtgagcagc tctctcccac
    10981 ggcagcctac cacctggtgc acgaacaggt gattagtccg ctacacagcc acctgacacg
    11041 cctgattgcc gcctggaccg gctgcgacgc caatgacacc cgcatgatcc ttcataccca
    11101 tgcgttgatt ggtgagattc tggcgttccg tcttggcaaa gaaacaattc tgttacgtac
    11161 cggctggacc gcgttcgatg aagaaaagac cgaactgatc aaccagacgg tgacctgtca
    11221 tatcgacctg attttgcaag gattattgca aaggagtttg tagtgatgaa aaaacctgtc
    11281 gtgatcggac tggcggtagt ggtacttgcc gccgtggttg ccggaggcta ctggtggtat
    11341 caaagccgcc aggataacgg cctgaagctg tatggcaacg tggatattcg tacggtgaat
    11401 cttagtttcc gtgttggggg gcgcgttgaa tcgctggcgg tggacgaagg tgatgcgatc
    11461 aaagcgggcc aggtgctggg cgaactggat cacaagccgt atgagattgc cctgatgcag
    11521 gtgaaagcgg gcgtctcggt ggcacaggcg cagtatgacc tgatgcttgc cgggtatcgc
    11581 gatgaagaaa tcgctcaggc cgccgcagcg gtgaaacagg cgcaagccgc ctatgactat
    11641 gcgcagaact tctataaccg ccagcaagga ctgtggaaaa gccgcaccat ttcggcaaat
    11701 gacctggaaa atgcccgctc ctcgcgcgac caggcacagg caacgctgaa atcagcacag
    11761 gataaattgc gtcagtaccg ttccggtaac cgtgaacagg acatcgctca ggcgaaagcc
    11821 agcctcgaac aggcgcaggc gcaactggcg caggcggagc tgaatttaca ggactcaacg
    11881 ttgatagcac cgtctgatgg cacactgtta acgcgcgcgg tggagccagg cacggttctc
    11941 aatgaaggtg gcacggtgtt taccgtttca ctaacgcgtc cggtgtgggt gcgcgcttat
    12001 gttgatgaac ggaatcttga ccaggcccag ccggggcgca aagttctgct ttataccgat
    12061 ggtcgcccgg acaagccgta tcacgggcag attggtttcg tttcgccgac tgctgaattt
    12121 accccgaaaa ccgtcgaaac accggatctg cgtaccgacc tcgtctatcg cctgcgtatt
    12181 gtggtgaccg acgccgatga tgcgttacgc cagggaatgc cggtgacggt gcaattcggt
    12241 aacgaggcag gacatgaatg atgccgttat cacgctgaac ggcctggaaa aacgctttcc
    12301 gggcatggac aagcccgccg tcgcgccgct cgattgtacc attcacgccg gttatgtgac
    12361 ggggctggtg gggccggatg gtgcaggtaa aaccacgcta atgcggatgc tggcgggatt
    12421 actgaaaccc gacagcggca atgccacgat gattggcttt gatccaatca aaaacgacgg
    12481 cgcgctgcac gccgtgctcg gctatatgcc gcagaaattt ggtctgtatg aagatctcac
    12541 ggtgatggag aacctcaatc tgtacgcgga tttgcgcagc gtcaccggcg aggcacgtaa
    12601 gcaaactttt gctcgcctgc tggagtttac gtctcttggg ccgtttaccg gacgcctggc
    12661 gggcaagctc tccggtggga tgaaacaaaa actcggtctg gcctgtaccc tggtgggcga
    12721 accgaaagtg ttgctgctcg atgaacccgg cgtcggcgtt gaccctatct cacggcgcga
    12781 actgtggcag atggtgcatg agctggcagg cgaagggatg ttaatcctct ggagtacctc
    12841 gtatctcgac gaagccgagc agtgccgtga cgtgttactg atgaatgaag gcgagttgct
    12901 gtatcagggg gaaccaaaag ccctgacaca aaccatggcc ggacgcagct ttctgatgac
    12961 cagtccacac gagggcaacc gcaagctgtt gcaacgcgcc ttgaaactgc cacaggtcag
    13021 cgacggcatg attcagggga aatcggtacg tctgatcctc aaaaaagagg ccacaccaga
    13081 cgatattcgc catgccgacg ggatgccgga aatcaacatc aacgaaacta cgccgcgttt
    13141 tgaagatgca tttattgatt tgctgggtgg tgccggaacc tcagaatcgc cgctgggtgc
    13201 aatattgcat acggtagaag gcacacccgg cgagacggtg atcgaagcga aagaactgac
    13261 caagaaattt ggtgattttg ccgccaccga tcacgtcaac tttgccgtta aacgtgggga
    13321 gatttttggt ttgctggggc caaacggcgc gggtaaatcg accaccttta agatgatgtg
    13381 cggtttgctg gtgccgactt ccggccaggc gctggtgctg gggatggatc tgaaagagag
    13441 ttccggtaaa gcgcgccagc atctcggcta tatggcgcaa aaattttcgc tctacggcaa
    13501 cctgacggtc gaacagaatt tacgcttttt ctctggtgtg tatggcttac gcggtcgggc
    13561 gcagaacgaa aaaatctccc gtatgagcga ggcatttggc ctgaaaagta tcgcctccca
    13621 cgcgaccgat gaactgccat taggttttaa acagcggctg gcgctggcct gttcgctgat
    13681 gcatgaaccg gacattctgt ttctcgacga accaacgtcc ggcgttgatc ccctcacccg
    13741 ccgcgaattt tggctgcata tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac
    13801 cactcacttt atggatgaag cagaatattg cgaccgcatc ggcctggtgt accgtgggaa
    13861 attaattgcc agcggcacgc cggatgattt gaaagcgcag tcggccaacg acgagcaacc
    13921 cgatcccacc atggagcaag cctttattca gttgatccac gactgggata aggagcatag
    13981 caatgagtaa cccgatcctg tcctggcgtc gcgtacgggc gctgtgcgtt aaagagacgc
    14041 ggcagatcgt tcgcgatccg agtagctggc tgattgcggt agtgatcccg ctgctactgc
    14101 tgtttatttt tggttacggc attaacctcg actccagcaa gctgcgggtc gggattttac
    14161 tggaacagcg tagcgaagcg gcgctggatt tcacccacac catgaccggt tcgccctaca
    14221 tcgacgccac catcagcgat aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa
    14281 ttcgcggtct ggtggttatt ccggtggatt ttgcggaaca gatggagcgc gccaacgcca
    14341 ccgcaccgat tcaggtgatc accgacggca gtgagccgaa taccgctaac tttgtacagg
    14401 ggtatgtcga agggatctgg cagatctggc aaatgcagcg agcggaggac aacgggcaga
    14461 cttttgaacc gcttattgat gtacaaaccc gctactggtt taacccggcg gcgattagcc
    14521 agcacttcat tatccccggt gcggtgacca ttatcatgac ggtcatcggc gcgattctca
    14581 cctcgctggt ggtggcgcga gaatgggaac gcggcaccat ggaggctctg ctctctacgg
    14641 agattacccg cacggaactg ctgctgtgta agctgatccc ttattacttt ctcgggatgc
    14701 tggcgatgtt gctgtgtatg ctggtgtcag tgtttattct cggcgtgccg tatcgcgggt
    14761 cgctgctgat tctgtttttt atctccagcc tgtttttact cagtaccctg gggatggggc
    14821 tgctgatttc cacgattacc cgcaaccagt tcaatgccgc tcaggtcgcc ctgaacgccg
    14881 cttttctgcc gtcgattatg ctttccggct ttatttttca gatcgacagt atgcccgcgg
    14941 tgatccacgc ggtgacgtac attattcccg ctcgttattt cgtcagcacc ctgcaaagcc
    15001 tgttcctcgc cgggaatatt ccagtggtgc tggtggtaaa cgtgctgttt ttgatcgctt
    15061 cggcggtgat gtttatcggc ctgacgtggc tgaaaaccaa acgtcggctg gattagggag
    15121 aagagcatgt ttcatcgctt atggacgtta atccgcaaag agttgcagtc gctgctgcgc
    15181 gaaccgcaaa cccgcgcgat tctgatttta cctgtgctta ttcaggtgat tctgttcccg
    15241 ttcgccgcca cgctggaagt gactaacgcc accatcgcca tctacgatga agataacggc
    15301 gagcattcgg tggagctgac ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg
    15361 ctgctgaaaa gcccacagga gatccgccca accatcgaca cacaaaaggc gttactcctg
    15421 gtgcgtttcc cggctgactt ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag
    15481 ttgatcctcg acgggcgtaa ctccaacagt gcgcaaattg ccgccaacta cctgcaacag
    15541 atcgtcaaaa attatcagca ggagctgctg gaaggaaaac cgaaacctaa caacagcgag
    15601 ctggtggtac gcaactggta taacccgaat ctcgactaca aatggtttgt ggtgccgtca
    15661 ctgatcgcca tgatcaccac tatcggcgta atgatcgtca cttcactttc cgtcgcccgc
    15721 gaacgtgaac aaggtacgct cgatcagcta ctggtttcgc cgctcaccac ctggcagatc
    15781 ttcatcggca aagccgtacc ggcgttaatt gtcgccacct tccaggccac cattgtgctg
    15841 gcgattggta tctgggcgta tcaaatcccc ttcgccggat cgctggcgct gttctacttt
    15901 acgatggtga tttacggttt atcgctggtg ggattcggtc tgttgatttc atcactctgc
    15961 tcgacacaac agcaggcgtt tatcggcgtg tttgtcttta tgatgcccgc cattctcctt
    16021 tccggttacg tttcgccggt ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt
    16081 aaccctattc gccactttac ggacattacc aagcagattt atttgaagga tgcgagtctg
    16141 gatattgtgt ggaatagttt gtggccgcta ctggtgataa cggccacgac agggtcagcg
    16201 gcgtacgcga tgtttagacg taaggtgatg taacttctta tctttcgcca gcaaagacac
    16261 taccgccggg ccggcaagga ttgccagccc tgcaattgcc agcaaaaagt tgttttccag
    16321 tacgcgggac aacagccaca gcacaatcag cgctgcgcca aaataccagg tcgtggtcag
    16381 ctcttccagc acgtctgcca cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa
    16441 catcatcact accgtgacac aatacagtgc gcataatccc acgccgacgt gaaccagagt
    16501 cttactcatc cagcccacca ccagcagcgc cactaccagt aaatgcaaca taatctgcca
    16561 gcaacttaga cccgttgcga cacgaacacg ttgttgccac ttcatggctt ctctcctgaa
    16621 gaatttatct ctacttactc agagtaccga actttacgga aaattccgta agaccgcacc
    16681 tttttgtgac gccgactaca ctatttcaat gagataagag gcaaagacag gaaaattatg
    16741 cccgatcaaa cacaacaatt ttcgttcaag gtgctcacca tcaacattca aaaaggtttt
    16801 accgcgttta accgacgctt cattttgccg gaacttcgcg acgcggtgcg taccgtcagc
    16861 gccgatattg tttgcctgca ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg
    16921 gaaaactggc ccgatacctc gcactacgag tttctcgccg acactatgtg gagcgatttt
    16981 gcctacggtc gcaatgccgt atacccggaa gggcatcacg gcaacgccgt actgtcgcgt
    17041 tatcccattg aacattatga gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc
    17101 gtgctctact gccgcattgt gccgccgatg accggaaaag cgattcatgt gatgtgcgta
    17161 catctgggcc tgcgtgaggc gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg
    17221 aatgagctac cggacggcga accggtattg gtggcgggtg atttcaatga ctggcggcaa
    17281 aaagctaatc atccgttaaa agtgcaggcc ggactggatg agatttttac ccgcgcccac
    17341 ggacgcccgg cgcgcacgtt tccggtgcaa tttcctctac tacgactgga caggatctac
    17401 gtcaaaaatg ccagcgccag cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt
    17461 tctgatcatg cccctttaag tgcggagatt catttatgaa atgtagctgg cgcgaaggca
    17521 ataagatcca gttgctggaa aacggcgagc aatattatcc cgcggtgttt aaggcgattg
    17581 gcgaggcaca agaacgcatc attcttgaaa cgtttatctg gtttgaggat aacgtcggca
    17641 aacagctaca tgcggcacta ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc
    17701 tggatggcta cggttcgccg gatctcagcg atgagtttgt caataaactg acggcagctg
    17761 gcgtagtgtt ccgctactac gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt
    17821 ttcgccggat gcatcgcaaa attgtggtga tcgacgcgcg tatcgccttt attggcgggc
    17881 tgaattactc cgccgagcat atgtccagct acggtccaga ggctaaacag gattacgcgg
    17941 tacgccttga agggccgatt gtcgaagata ttctccagtt tgagctggaa aacctgcctg
    18001 gacagagcgc ggcacggcgc tggtggcgac gtcatcacaa agcggaagag aaccgccagc
    18061 cgggagaagc gcaggtattg ctggtctggc gcgataacga agaacatcgc gatgatattg
    18121 aacgccatta tctgaaaatg ctcactcagg cgcggcggga agtgattatc gccaacgcct
    18181 acttcttccc cggctatcga tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc
    18241 ggatcaaact gatcattcag ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct
    18301 tgctgtataa ctatctggtt aaaggcggcg ttcaggtttt tgagtaccgc cgccgcccgc
    18361 tccacggcaa agtggcattg atggacgatc actgggcgac agtagggtcc agtaatctcg
    18421 atccgctcag tttgtcactg aatctcgaag caaatgtcat catccacgat cgtcatttta
    18481 accagacgct gcgcgataat ctgaacggca ttattgccgc agattgtcag caggtggatg
    18541 aaaccatgct gcccaaacgc acctggtgga acctgaccaa aagcgtgctg gcgttccact
    18601 ttttacgcca cttcccggcg ctggttggct ggcatccggc acacacacca cgtctgacgc
    18661 aggttgatcc ccccgcacaa ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca
    18721 cgggggtaaa accctgataa gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct
    18781 cacctggctg ttttttatcg cggtgattgt gttgctggtg gtctacgcca aaaaagtgga
    18841 ctgggaagag gtctggaagg tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt
    18901 cgggctggtg gtcgtcagct atctgattta cggctgctat gacctgctcg cccgctttta
    18961 ctgcggtcac aaactggcga agcgccaggt gatgctggtg tcgtttatct gctacgcctt
    19021 caacctgacg ctcagtacct gggtcggcgg cattggtatg cgctatcgtt tgtactcccg
    19081 actagggtta ccgggcagca ctattacgcg gattttctcg ctcagtatta ccaccaactg
    19141 gctgggctat attttactgg cagggattat ctttaccgca ggcgtggtgg agttgccgga
    19201 tcactggtat gtcgctcaaa ctacgctgcg tattctcggc attggcttac tgatgattat
    19261 cgcggtttat ttgtggtttt gcgctttcgc gaagcaccgc catatgacca tcaaagggca
    19321 aaagctggtg ctgccttcat ggaaattcgc cctcactcag atgctgattt ccagcgttaa
    19381 ctggatggta atgggggcga ttatctggct gttacttggt caaagcgtga actatttctt
    19441 tgtactgggc gtgttactgg ttagtagtat tgctggcgtc atcgtgcata ttccggcggg
    19501 gatcggtgtg ctggaagcgg tgtttatcgc actactggct ggggagcata cctcgaaagg
    19561 gagcattatc gccgcgctgc tcgcttatcg cgtcctgtat tactttatcc cgctgctgct
    19621 ggcgctgatc tgttacctgc tactggaaag ccaggcgaag aagctacggg cgaaaaatga
    19681 agcggcgatg tgaagtttag ttaagttctt tagtatctgc attcacgact aatgaaaaaa
    19741 acgcgtatgc ctttgccaga caagcgttat agctatcgcg gcaagtatcc caaagaagcc
    19801 gataaaatag taatagagtg ttaatgcacc tgtaaaagcg caacgaaccg gactccacaa
    19861 ctcttcacta tggcattttc ctgccagttc aaggagctta tttcgtccat gcgagataat
    19921 gcctggccat aacacaatca ctaagcaact tattacccaa atgggccgtt cactcattaa
    19981 tgccgcattc acgatgatta ccagggccaa ccctgtcaac gtcatcataa tgataaaacg
    20041 gtgagaacca ggatcaacat taaataataa accggccagc atactgatta aaaacatcgc
    20101 gcccgcagtg ctgaacacaa tgataatggc atatacagga actaaacttg caacgacaat
    20161 cgccaccaaa gagagtaaaa acagatatat tacgcctgcg atgacggcat ttatcttgtt
    20221 gatatcctta cttaagccat acatacaaac taataataac cctatgccga catacattgt
    20281 tggtgaatca tatttggttg ccgtaatttc gagatactta gctccccagg ccagtcctgc
    20341 ggttgccacc aaacctaaag cggtaatgag ccatataccg tttaatatcc tggtctcgtg
    20401 ctgaaaatct aatttattgc tgttttgtga gtatgattcc attcttttag ttgtccttag
    20461 aaaaaatatt aatattcacc atcaataaat ttaaacccac gcatacaaaa tacttttgta
    20521 tttatggata cggaaaacgc ttccgctcgg ccataaggcg tattttatac ctggtacttg
    20581 ttaagtgtcg tttttagtaa gaaaagaaat cccgacacat ggccgggata ttgatggtga
    20641 aaaagaatta acggcggttg ccaaaaattc gcagcaacat caggaacagg ttgatgaagt
    20701 ccagatacaa ggttaacgcg ccaagaatgg aatatttgcg caggttcgac gtgtcgcggg
    20761 tatcaatctg ctcacccata tttttcaatt tctgagtgtc atacgccgtc aatccgacaa
    20821 agacaatcac gccgatgtag gtaactgccc acatcaatgc ttcgcttttc agccagaagt
    20881 tgaccagcga tgccagcaca atgccgatta acgccataaa cagcatattg ccaaagccac
    20941 ttaaatcgcg cttcgtggtg taaccgtaca ggctcattgc accgaacatc ccggcagtaa
    21001 cgacgaaagt actggcgata gaagcagcgg tatagacaat gaatatactg gaaagcgtaa
    21061 gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt
    21121 gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa
    21181 agacacggtt agtgaacaac agctccatca cggccgcgga attagccgca taccaggcaa
    21241 caaatgcggt cagcaacaag ccaacggtca tccagccata gacttgcgcc atataagttt
    21301 gcaagccagc ccggggttgt acgattgaat cagaacgtgg gaatctgtcc atgacgatct
    21361 cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc
    21421 agcgtaaacg catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta
    21481 acaaaaaact accagcgttt tgccgcctgc tgatcgctct cccgagcttc aacccagcgg
    21541 tcgccttccg gcgtggcttc gcgcttccag aacggtgcgc gggttttgag ataatccata
    21601 ataaactgcc cggcttcaaa cgcactgctg cgatgcgcac tggtgacacc gacaaaaacg
    21661 atttcatcgc ccggccataa ttccccgatg cggtgaatca cagtgacgcg ccccagcggc
    21721 cagcggttac gcgcttcatc aacaatttct gccagtgctt tttcagtcat ccccggatag
    21781 tgttcgaggc ttaatgcttt gacgctgtca cccaggttat ggttgcgcac cttaccagta
    21841 aaggtgacta ccgcaccgtc ttcgtcacgc tccgccagcc acgggtactc ttctcctacg
    21901 ctgaacggct gcggaccaac aacaattttg gtttctgcca tcttaacctc cggttaccgg
    21961 cgggaagaaa gctacttcgt cgccgtcagt cagcggatgg tcaaaactca ccagcgtctg
    22021 gttgacggca gccagtaatt tgccatcttc cagcgccagc gcccagcgat cgctctgcgc
    22081 agccatgtgc tggcgtaacg cttcaacagt tgggaaatcc gcagccactt cggttgcatc
    22141 tgttcccacc aactcgcgca cctgtgcgaa aaaaagaact ttaatcatcc gcttccacct
    22201 taaagtcacc cgacttgccg ccgctcttcg ccagcaaacg taccggacca atcaccatat
    22261 ctttttgcac cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta
    22321 atgcttccat ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgcaccc
    22381 gattgtgctc cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcggatgac
    22441 agagcgggat cagatcccag gtgcgttttg ccgcctgaat accggcaata cgcgcagtgg
    22501 caaatacgtc gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca
    22561 tggtgacaaa ggcttccgcc cgcgcttcac gcacggtttc cgctttggcg gagacatcca
    22621 ccatgtgcgc ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa
    22681 atgtggatgg aaattacacg gacacgtacg ggcatccagc tgcagcgcga tgatattttc
    22741 ccatgcggta cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttggc
    22801 gacgcccgct accgcacgag attgcaacgt ggaagtgcca atctcttcaa acgacaacat
    22861 ccggaacact tcaccaaaac cttcaacttc acggtcgaac aacggcagca atgcttcggg
    22921 agcctgatca ccttccgtca ggccagtacc acccgtaatc aataccactt gtacatcgtc
    22981 gctggcgatc cacgcagata cctgagcgcg aatagcgtag cggttttctt tcacaatggc
    23041 tttatcgaca acgtgatggc ccgcttcttg cgccgaatcg cgcagatagt gaccggaggt
    23101 atcgtcttct tcaccgcgac gattagaaac cgtaagaata gcaatacggg tcgggataaa
    23161 ttcagtgctt acctgactca tctgatctct ccttttgacg ttttagccgc caatgtacga
    23221 taaattttgc gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt tcttctcccg
    23281 cagcgccgct gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt ccagcagatc
    23341 gcgcaggtta acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac
    23401 gcgcaggcgg ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa ggccaatctc
    23461 tccggcgtaa tctggatggc aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg
    23521 gtggatccag ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga tgtgatgctt
    23581 acggaagagc tcgctgccct cgcccgtttc catcagttcg atgaaacgca gctggatagg
    23641 gcgatgctgg atccagttca gaaaggtgtc gagctggtga tgattaacat cacgcatcag
    23701 cacggtattg actttgacct tctcaaaacc ggcctcaaat gcagcatcaa tccctgccat
    23761 gacctggttg aatttatcct gcccggtaat agcgtgaaac tggcgggcgt ccagactgtc
    23821 gacactgacg ttaatgccag taagtcccgc atcgcgccag ttcgccacat cgcgttccag
    23881 acggtaacca ttggtggtga ccgcaatctg gcggatagcg tcgttttccc gcacagcggc
    23941 gatgatatcg gtaaagtcgc ggcgtaaaga cggctcacct cctgtcaggc gcactttttc
    24001 ggtgcccaga ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg taagaaagcc
    24061 tttattggtg acgccgctcg gtttgtagcc atccggcagg cagtaggtgc aacgaaagtt
    24121 acacacatcg gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag
    24181 ttgtgaagcc atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct
    24241 ggtggcaatc tttacgagca ttgtcacggc caaagcaccg tatcagttga cccaggtgca
    24301 gaggctagag tgtttagtgg ttaagccgat actagcgtgt aaatgagatt tttaccatcc
    24361 acactttcgc tatgcaatca tgtatatagc gtcatgatcg tgctatttcg ctacagaata
    24421 agggaaaatg gcagttttac tttgatatac atcatcaggg aggcatagcg tgcatcgtcc
    24481 tttagaggta gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttccc
    24541 cgcaatttgc gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt
    24601 acgctggctg atcttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt
    24661 ctctcatcac tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac cacggataat
    24721 ggtggctcga cggggcgtat tcgccgttca gaaggcggca ttgcctgggg cgatatgcgc
    24781 aactgcctca accaactgat aacggaaccg agcgtcgcct ccgcgatgtt tgaataccgt
    24841 tttggcggca atggcgaact ttccgggcac aaccttggaa acttgatgtt aaaggcgctg
    24901 gatcacctta gcgtgcggcc tctggaagcc atcaatttaa ttcgtaatct gctgaaagtg
    24961 gatacgcatt tgattccaat gtcagagcat cccgtcgatc tgatggcgat tgacgatcag
    25021 gggcatgaag tttacggcga ggtcaatatc gaccagttaa ctacgccgat tcaagagtta
    25081 ttattaacgc cgaatgtacc cgcaacgcgt gaggcagttc acgctatcaa tgaagcggat
    25141 ctcatcatta ttgggcctgg cagtttttat accagcctga tgccaattct gctgctgaag
    25201 gaaatcgccc aggcattacg ccgcacgcca gcgccgatgg tttatatcgg caatctgggg
    25261 cgtgagttga gtttacctgc ggctaatttg aagctggaaa gcaagctggc aattatggag
    25321 cagtatgttg gtaaaaaagt cattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg
    25381 gcggtgaaag agcggattgt gatccaggag gtactggagg ccagcgatat tccgtatcgt
    25441 catgaccgcc agttgttaca taacgcgctg gaaaaggcgt tacaggcttt aggttaacgg
    25501 aaatccgaag gaaaattccg gcttcctatt gaagacaaag tgcgcgttgt ttatgccgga
    25561 tgcggcgtga acgccttatc cggcctacaa accgcgcaaa ttcaatatat tgcggagaaa
    25621 atgtaggcct gataagcgta gcgcatcagg ctgttttccg tttgtcatca gtcttcttcg
    25681 ctatcctgtt acgatgcggc aataaacaac tcacgcaact gatgcaactg gtcacgaatt
    25741 tgtgccgctt cttcgaactc cagattctgc gcgtgttgca tcatcagccc ttccagttca
    25801 tggattttct gctgcaacgc tttcggcgac atatccatcg gcacattatc cggctcaaca
    25861 atcgggcgcg attttcctct gcccttcgct ttggttttgg caatgttctg ccccagcgcc
    25921 aggatatcga ccactttctt gttcaagcct tgcggcgtaa tgccgtgttc ctcgttgtac
    25981 ttctgctgtt tctcacggcg acgttcggtt tcgccaatcg ctttcgccat tgatggggtg
    26041 atcttatcgc cgtagagaat cgctttaccg ttaacgttac gtgccgcacg accaatggtc
    26101 tggatcaacg aacgttcgga acgcaggaag ccttctttgt cagcgtcgag gatcgccacc
    26161 agcgacactt ccggcatatc cagaccttcg cgcagtaagt tgatccctac cagcacgtcg
    26221 aactcaccca gacgcaagtc gcggataatc tccatacgtt cgacggtgtc gatatctgag
    26281 tgaagataac gcacgcgctc gccatgttct tcgagatatt cggtaagatc ttccgccatc
    26341 cgcttggtca gtgtggtgac cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc
    26401 gaaagaagat catcaacctg tgtcgccacc ggccgcactt cgataatcgg gtcaagcaat
    26461 ccggttggac gcaccacctg atccaccaca tcgccgccgg atttttccag ctcgtaatta
    26521 cccggcgtcg ccgaaacata gatggtttgc ggcgctaatg cttcgaactc ttcaaactta
    26581 agcggacggt tatccagcgc tgatggcagg cggaagccgt actccaccag tgtctcttta
    26641 cgcgcccggt caccgcgata catgccgcca atttgtggaa tggtgacgtg agattcatcg
    26701 acgaccagca gcccatcggc aggcaggtaa tcaaacagcg tcggcggtgg ctcacccggt
    26761 ccacgaccgg agaggaagcg cgagtagttt tcaatccccg aacagtagcc cagctcgttc
    26821 atcatctcca gatcaaactg ggtacgctgg gtcagccgct gctcttccag cagtttgttg
    26881 ttttccaaca gcactttgcg tctggcggcc agttcttctt tgatctcctc catcgcctgt
    26941 acgatgcgct cgcgcggtgt gacgtaatgc gttttcgggt agatggtaaa acgtggaata
    27001 gtggaaacaa tctgcccggt cagcgggtca aataacgaca atcgttccac ttcctcgtca
    27061 aacagttcca cgcgaagtgc aatgtcatcc gattctgccg ggaagatatc aatcacctcg
    27121 ccacgaacgc ggaaagtacc acgctggaat gcttgatcat tacgagcgta ttgcagctcc
    27181 gccagtcggc gcagaatcgc gcgctgatcg ataatcatac cgaccgtgag atggagcatc
    27241 atcttgagat ataaatcagg atcgcccaga ccataaatcg cggaaacaga ggccactaca
    27301 accacatcac gccgctccag catcgctttg gtggcggata aacgcatctg ctcaatatgt
    27361 tcgttaaccg aggcatcttt ctcaatgaaa gtgtcggaac tcggtacata ggcttccggc
    27421 tgatagtagt cgtagtagga gacgaaatac tccaccgcgt tttccgggaa gaactctttc
    27481 atttcgccat acagctgggc cgccagcgtt ttgttgggcg caagtaccat ggttgggcgc
    27541 tgaaggtcag caatgacatt ggcaatggtg aaggttttcc ctgagccagt cacgccaagt
    27601 aacgtctggt gcgccaggcc atcttccagc ccctcttcga gacgtcgaat cgcctctggc
    27661 tgatcgccag aaggtttaaa agcggaattc agtttgaacg gtttactcat gagtcgctac
    27721 ctgaaggagt tgggcgggca ggtatgtaat tttactcgtc gtacttaatt atgccaacaa
    27781 attatactgg ataaaaaaac agttcatcac cataatattt ctgatacagc gtaaactccg
    27841 ctgtcaatca tgagcaaaat ttactctgtg gcgagataaa actccggcct taccgggtta
    27901 tccccaaatc aacggctttt ttaacatttg tcaagatgag tgatgacagt tttatggcaa
    27961 gagatgcctg ttcagtgact gccattgcat ttatctaacc agttaaaaaa taaaagatat
    28021 ttctttgagc cgtctttaac gccaattcgc gtgcaagccg cgtattctct cgcttgcctc
    28081 gtgttttcta actctaatac acatggttat ccacaggaat agtggataac tgtctccagc
    28141 ccctatcccc cgccgcctgg gcaattccca cattcgccgc atgaggcgga agtaaaattt
    28201 ttttcgtgat tattttagaa tttaattggt taaattgcag tcaatcgaag acgcgatctc
    28261 gctcgcaatt taaccaaata cagaatggct acaacaaggc aaggtttatg tactttccgg
    28321 ttgccgcatt ttctggattt tctgcaagcc aggggatctc tcccagcagc ggcgcgggaa
    28381 tcatgcgggt gagcgtggtc atatattcag cgtgacgttt tcccggaggc gtaacatcgt
    28441 tcgccaccca acccgccaga gtcagtccgg cgtattgtat tgcctgcgca gtcaacatcg
    28501 cgtgattaat acagccgagt ttcacaccaa ctaccagtat caccggcagt tgttcctgtg
    28561 ttacccaatc tgcaaaagtg aaagtgtcag aaagcggcgt aaaccagccg ccagcacctt
    28621 ccactaacac ccagtcagcc tgttgttcaa gcgcgcgtaa tccggcgctc attaccaatg
    28681 attctatcgg cctgccctct tgcgcgctga tgatgtgcgg cgaagtgggt tctgcgaagg
    28741 tgtaaggatt tactgttgcg taatccagct gcaggctgct gttgtgctgt aacgccagcg
    28801 cgtcactatt acgtaagcct tccggggtct tttcgctgcc agaggcgacc ggtttataac
    28861 ctgccgtccg ataacctact gccttcgcgg cttgtaaaag tgcacaactg gcgacggttt
    28921 tccccacttc ggtatccgtt ccggtgacaa aataacgttt actcacgagc aatcactccc
    28981 aaaaaaagat gatacgtcag aggatatcgc ccctgttgtt gcggccaggc cagttgcaat
    29041 cgctgcaatt gcgaacgcgt taatattcgc gggtcgcgcc cttcatgaag atgcgtagca
    29101 ccgatgcctt tcagcgaacg catggcactg agcgcatcat caaaccatag tgtgatgggc
    29161 tgaatatgat gttgataatg cacgccgttc agcgactgtt cgatttcatc tggcggtaaa
    29221 aagcgattag catgcggacg ctcgtccacc gcctgccacg cctgatgcag ttcgggtaac
    29281 gatccctgca ccagcgtggt aaacgccacc acgcctccgg ggcgcaccac ccgatacagc
    29341 tcgcggagtg ccgtggataa attaccgcac cactgcaccg cgagattgct ccatgcaaga
    29401 tcgaacgtcg cagtcgctaa cggcagggat tcgatatctc ccgccagata atggtctgca
    29461 gcatctttct ggcgggcttg cacaagcatt ggcggtgaga gatctaacgc cgtcacctgt
    29521 gcgtgacgct ctcgccagtg gcggctcatc cagcctggcc cacaccccgc gtccagtacg
    29581 tgggtgtatt tacgctgtgg gagcattgcc agtaagacgt cagcactccg gcgctgtaac
    29641 tctgcgtgtt gctcatagtg cgcggctgcc cgaccaaatg ccgctgcaat ggcttgttta
    29701 ttaaccgttg ccatgcagca cctccagcag acggtcgata tcctgcattt catgcgctgc
    29761 ggttagcgtt aagcgcagtc gcgcagtacc agcgggtacg gttggcgggc gaatcgccgt
    29821 gacccagcag ccttgctggc gcagtttttc tgccagttgt aacgcacggc tgttatcacc
    29881 gacgatcaat ggctggatgg cgctgcatga atcagcaagc gtaaacggca aatcctgtac
    29941 tccggcacga aaacgcgcaa ttagtgacac cagtttttcg cgccgtgcat ccccctcatc
    30001 acggcgaatg accgccagcg acgcacgtaa tgcctgcgcc tgagcgggcg gcatactggt
    30061 gttgtagata aggtggcggg cgaattgcag cagataatcc gccaccgtac tggagcaaag
    30121 caccgctgcc ccgctgacgc caaatccttt gccaaaagtc accaccagca attctggttt
    30181 taccttttgc agccagcagc tgccgcgccc ctgctccccg ataacgcccg tgccgtgggc
    30241 atcatcgacc atcaaccagc cattgtgctg ttgcgttacc tgctggattt ccgccagtgg
    30301 cgcactatcg ccgtccatgc tgaacacgcc ttctgtcacc accagttgct gccccggaca
    30361 gggggaagca agcagtcgcg ccagatgagt gacatcgtta tgaacaaaac ggcgaagctg
    30421 cgacgggctt aaactggcag cctccagcaa tgaggcatgg ctaagccggt cagcgacaat
    30481 gcggtcctct tttgccatca tcgcggcaat gaccgcctga ttagcggcaa aaccagagat
    30541 aaatagcagt gcccgcgaat agcccagcca ctcggccagt tcttcttcca gcgcctgatg
    30601 cgcaacgcta taaccgctga cgtgaccgga accgccgcta ccgacgccaa attgctccgc
    30661 accctgctgc caggcacgga taatttgcgg atgatggctt aaacctaaat aatcgttact
    30721 ggaaaagttc agatactggc gatcatccgc caccagccag cgtccggctc cttgcgccac
    30781 tggataacgg cgacgcaggg catcggcagc acgccgcgca tcgagcgccg cgttgatttt
    30841 ctcctgccag ctcatagtgc tgccgcgttg taatattcgt cagtatccgg ggtcatcagc
    30901 gcctgttcaa gacgttgctg ttgttcgtta tcccctgcca gcacggcagt ttgctgcgga
    30961 tttagcccca gtttgcggaa cagttgcagg tctttatctt cttccggatt cggcgtggtc
    31021 agcagtttgc aaccgtagaa aatcgagttt gcgcctgcca taaagcacat cgcctgagtc
    31081 tgttcgttca tctgctcgcg tccggcagaa aggcgcacgt aagaggtcgg catcatgatc
    31141 cgcgcgaccg caatggtgcg aataaaatca aaggcatcaa cgtcatcgtt atcggcaagc
    31201 ggcgtgcctt tcaccttcac cagcatgttg attggcacgc tttccggcgg cgtcggcagg
    31261 tttgccagtt gcagcaataa tccggcgcga tcttttaccg tttcgcctaa gcccacaatg
    31321 ccgccagaac agactttgat cccggcatcg cgcacttttt ccagcgtatc gaggcgttcc
    31381 tgataagtgc gggtggtgat gatattgccg taaaactccg gcgaggtgtc gaggttgtgg
    31441 ttgtagtaat ccagcccggc gttcgcgagg cgctgcgcct gagattcact caacgtgccc
    31501 agcgtcatac acgcctccag ccctaaatct tttaccccat gcaccatctg ctccaggtac
    31561 ggcatatcgc gttcgttggg attcttccac gccgcgccca tgcaaaaacg cgtcgatccc
    31621 gccgctttcg ctttgcgcgc cgactccagc acctgttcaa cttccatcaa ccgctcggct
    31681 tccagcccgg ttttatagcg cgagctttgc gggcaatatt tgcaatcttc cgggcaagca
    31741 ccggttttaa tcgacagcaa cgtgctgacc tgcacctgac gaggatcgaa atgctggcga
    31801 tgcacctgct gcgcttcaaa cagcagatcc agcaacggtt tttcaaataa ttctgcgact
    31861 tgcgacaatg tccagcgtgg gcggtgagcc atggggcttc tccaaaacgt gttttttgtt
    31921 gttaattcgg tgtagatttg taaacctaaa tcttttcaat ttggtttaca agtcgattat
    31981 gacaaaggac gatcttgcct ttgaccaacg ccatatctgg cacccataca catccatgac
    32041 ctccccgctg ccggtttatc cggtggtgag cgccgaaggt tgcgagctga ttttgtctga
    32101 cggcagacgc ctgattgacg gtatgtcgtc ctggtgggcg gcgatccacg gctacaatca
    32161 cccgcagctt aatgcggcga tgaagtcgca aattgatgcc atgtcgcatg tgatgtttgg
    32221 cggtatcacc catgcgccag ccattgagct gtgccgcaaa ctggtggcga tgacgccgca
    32281 accactggaa tgcgtttttc tcgcggactc cggttccgta gcggtggaag tggcgatgaa
    32341 aatggcgttg cagtactggc aagccaaagg caaagtgcgc cagcgttttc tgaccttccg
    32401 caatggttat catggcgata cctttggcgc gatgtcggtg tgcgatccgg acaactcaat
    32461 gcacaacctg tggaaaggct atctgccaga aaacctgttt gctcccgccc cgcaaagccg
    32521 catggatggt gaatgggatg aacgcgatat ggttggcttt gcccgcctga tggcggcgca
    32581 tcgtcatgaa atcgcggcgg tgatcattga accgattgtc cagggcgcag gcgggatgcg
    32641 catgtaccat ccggaatggt taaaacgaat ccgcaaaatg tgcgatcgcg aaggtatctt
    32701 gctgattgcc gacgagatcg ccaccggatt tggtcgtacc ggcaaactgt ttgcctgtga
    32761 atatgcagaa atcgcgccgg acattttgtg cctcggtaaa gccttaaccg gcggcacaat
    32821 gaccctttcc gccacactca ccacgcgcga ggttgcagaa accatcagta acggcgaagc
    32881 cggctgcttt atgcacgggc caacttttat gggcaatccg ctggcctgcg cggctgcaaa
    32941 cgccagcctg gcgattctcg aatctggcga ctggcagcaa caggtggcgg ctattgaagt
    33001 acagctgcgc gagcaacttg cccccgcccg tgatgccgaa atggttgccg atgtgcgcgt
    33061 gctgggggca atcggcgtag tcgaaacaac tcgtccggtg aatatggcgg cgctgcaaaa
    33121 attctttgtc gaacagggag tatggatccg gccttttggc aaactgattt acctgatgcc
    33181 gccctatatt attctcccgc aacagttgca gcgtctgacc gcagcggtta accgtgcggt
    33241 gcaggatgaa acattttttt gccaataacg ggaagtcagc gtgtgggttt ctggctacac
    33301 tttctgcaaa ctagaaagga gggttcatga aactcatcag taatgatctg cgcgatggcg
    33361 ataagctgcc gcatcgtcat gtctttaacg gcatgggtta cgatggcgat aatatttcac
    33421 cgcatctggc gtgggatgat gttcctgcgg gaacgaaaag ttttgttgtc acctgctacg
    33481 acccggatgc gccaaccggc tccggctggt ggcactgggt agttgttaat ttacccgctg
    33541 atacccgcgt attaccgcaa gggtttggct ctggtctggt agcaatgcct gacggcgttt
    33601 tgcagacgcg taccgatttt ggtaaaaccg gatacgatgg cgcagcgccg ccgaaaggcg
    33661 aaacccatcg ctacattttt accgttcacg cgctggatgt agagcatatt gatgtcgatg
    33721 aaggagccag cggcgcgatg gtcgggttta acgttcattt ccactctctg gcaagcgcat
    33781 cgattaccgc gatgtttagt taatcactct gccagatggc gcaatgctat ctggtatcac
    33841 ttaaaggtat taaaaacaac tttttgtctt tttaccttcc cgtttcgctc aagttagtat
    33901 aaaaaagcag gcttcaacgg attcattttt ctatttcata gcccggagca acctgtgaac
    33961 acattttcag tttcccgtct ggcgctggca ttggcttttg gcgtgacgct gaccgcctgt
    34021 agctcaacac cacccgatca acgtccttct gatcaaactg cgcctggtac ctcttctcgc
    34081 ccgattctgt cggcaaaaga agcgcagaat ttcgatgctc aacactattt tgcatccctg
    34141 acaccaggtg ctgcagcgtg gaatccttcc ccgattaccc tgcctgcgca acctgacttt
    34201 gttgtcggcc cggcgggcac tcaaggtgta acgcatacca cgattcaggc ggcggtagat
    34261 gcggcaatta tcaagcgtac caacaagcgc cagtatattg ccgtgatgcc tggtgagtat
    34321 cagggaacgg tgtatgtccc tgctgctccg ggtggaatta ctctgtacgg tacaggtgaa
    34381 aaaccgattg atgtgaagat tgggctttcc cttgatgggg gcatgagccc tgccgactgg
    34441 cgtcacgacg tcaacccgcg cggcaaatat atgccaggta aaccagcgtg gtatatgtac
    34501 gatagctgcc agagcaaacg cagcgacagt atcggtgttc tctgctctgc ggtcttctgg
    34561 tcacaaaaca atggcctgca actgcaaaac ctgaccatcg aaaacacgct gggcgatagc
    34621 gtagatgcag gtaaccatcc ggcggtggca ctgcgtactg atggtgacca ggtacagatt
    34681 aacaacgtta acattctcgg tcgtcagaac accttctttg tcaccaacag cggtgtgcag
    34741 aaccgtctgg aaacgaatcg tcagccgcgt acgctggtga ccaacagcta cattgaaggg
    34801 gatgtggata tcgtttctgg tcgcggcgca gtggtgttcg ataacaccga attccgcgtg
    34861 gtgaactcac gtactcagca agaagcgtat gtgtttgcac cggctacgct gtccaacatt
    34921 tactacggtt tcctcgccgt aaacagccgt ttcaatgctt tcggtgatgg tgtggcgcaa
    34981 ctgggccgct cgctggatgt tgatgccaat accaacggtc aggtggtgat ccgtgatagc
    35041 gccatcaacg aaggttttaa cacggctaaa ccgtgggccg atgcggtgat ctctaatcgt
    35101 ccgtttgcgg gtaataccgg cagcgtagat gataacgacg aaatacagcg caatctgaat
    35161 gacactaact acaaccgcat gtgggaatac aataaccgcg gcgtgggtag taaagtggtt
    35221 gcagaggcga agaagtaaga gcaattaact atttgccgga tgcggcgtaa acgccttatc
    35281 cggcctacgg ttcgatgcga tttgtaggtc ggataagatg tgcaagcatc gcatccgaca
    35341 ataagtgccg aatgcgacct acattcacat ggcgcttttt acatctgacg gtttttattg
    35401 aagttaatca aactacccgc cttgataatc tcgcgctctt cagcagtcag actttccata
    35461 cagagcgtaa tttccgttac cggcgcatct tcatggatca cataaccttt aaacgtcgta
    35521 cccggattat ccagcgccgc tttaatgcca gggatgtaaa tgtaatcccc cacttcaaag
    35581 gttggcactt ccgccatctg cagcggtaac atcccccagt tgatgacgtt agaacggtag
    35641 cgtttagtcg cgtactcttc ggcaatattc gccagaccgc caatcacacg ctggcagctc
    35701 gccgcctgtt cacgcgcaga accatcgcct ggtttcaccg catataccat gctgccaatt
    35761 tcagtttgca gcggatcaat atgctcctga ccagcaatct gcttaatgcg cgcaaacacc
    35821 tctgtcagct cgctgacatt ccccgccaga cgctgatttt ccagctcagc agtcgcttta
    35881 cttctgccaa cataaccggg atcgcggcgt gacagggtaa actccgccag accaatcgga
    35941 tttgaacgat aagaagaggt ttcaccggaa ggaatcagtt cgtcggtggt ggtcacttcg
    36001 tcgaggatct tcgagcacac tttcaggacg atattgtcag tcagcgcacc caattccggc
    36061 cagtctttaa tgttcggtcc gtaaatcagc ggttgctgag ttgccccttt cacaaagccc
    36121 tgataaacac ggtttttata cggcgttaca tcgaaggcgt actccggcac gttgtcccag
    36181 caatcaagtt cgctggcaga ggttaaatag ccaccgtttg ccgcagtcgc agcgatagaa
    36241 cgagcgtcca tcaacgccac cgctgacatc tgcccattag ctggcttaga gccttcgcgg
    36301 ttcgggaagt tacgcgtggt gtggcgaata cttaatccgt tgttgattgg cgtatcgccc
    36361 gcgccaaagc atgggccgca gaacgcggtt ctgatgattg cgcctgcgcc aatcaaatct
    36421 gctaccacac cttttttggc taaatccata aacaccggct gtgatgacgg gtaaaccgcc
    36481 agcgagaagg tgtcattgcc acaggattga ccgcgcagtg cattcgccgc cgcgatgacg
    36541 ttttcgtagt taccgccaga acagcccgcg ataatcccct gctgcacttt caggcgacca
    36601 ttttccactt tatccagcag cgagagtttg gctttaccgt gcgccacgcg ttcggactca
    36661 atttcaatct cacgcagaat gtcggtcagg ttctggttca gtgtgtcgat ttcatacacg
    36721 ttgctcgggt ggaacggcag cgcaatcatt ggtttgatgg cgcttaaatc aacgctgatg
    36781 cagccatcgt agtacgccat cggttgaggg ttaagctggc agtaatcctg gccgcgaccg
    36841 tgcagcgcca gccagttatg cacttcttca tcagtttgcc agacggagct taaacaggtg
    36901 gtttccgtgg tcatcacgtc aacgctgtta cggaaatcgg tagagagcgc gctaacgccc
    36961 ggtccaacga actccatgac tttgtttttg acgtaaccgt ttttgaacac cgcgccaatg
    37021 atagccagcg ccacatcctg tggccccaca tacggcgcag gttttccggt cagatgcacc
    37081 gcaaccacgc ccggatagtc gatatcctag gtgtcattaa gcagctgttt taccaactca
    37141 ccgccgccct caccgactgc cattgtccct aatgcaccgt aacgggtgtg gctgtctgac
    37201 ccgaggatca ttttgccccc gcctgccatc atctcacgca tatattgatg gatgaccgca
    37261 atatgcggag gcacaaaaat accgccataa cgctgagctg ccgataaacc aaaaacatgg
    37321 tcatcaccgt taatggtgcc gccgacggcg cagagtgagt tatggcagtt ggtcagcaca
    37381 tacggcagcg ggaaacgttc cattccggac gctttagcgg tctgtacaat accaacaaag
    37441 gtaatatcgt gagaggctaa tgaatcaaac ttaattttaa gtttatccat atttccggac
    37501 gtattatgag aagagagaat agaccaggca atagtgcctt ttttggcttc ttctttttta
    37561 atttcgccgg tgaaatgttc ttcggcaatt atttcgttat tactggcgag aaacacgcct
    37621 ttttcaggta atga
//
LOCUS       DAJTMK010000019        36514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTMK010000019 DAJTMK010000000
VERSION     DAJTMK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36514
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.019"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(29..1753)
                     /locus_tag="OO135_000816"
     CDS             complement(29..1753)
                     /locus_tag="OO135_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5396759.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIVWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            2132..3754
                     /gene="pckA"
                     /locus_tag="OO135_000817"
     CDS             2132..3754
                     /gene="pckA"
                     /locus_tag="OO135_000817"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5396760.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(3830..5182)
                     /gene="envZ"
                     /locus_tag="OO135_000818"
     CDS             complement(3830..5182)
                     /gene="envZ"
                     /locus_tag="OO135_000818"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5396761.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGSSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5179..5898)
                     /gene="ompR"
                     /locus_tag="OO135_000819"
     CDS             complement(5179..5898)
                     /gene="ompR"
                     /locus_tag="OO135_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5396762.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            6126..6602
                     /gene="greB"
                     /locus_tag="OO135_000820"
     CDS             6126..6602
                     /gene="greB"
                     /locus_tag="OO135_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5396763.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            6699..9020
                     /locus_tag="OO135_000821"
     CDS             6699..9020
                     /locus_tag="OO135_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5396764.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSSKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLCLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILTATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            9458..9685
                     /gene="feoA"
                     /locus_tag="OO135_000822"
     CDS             9458..9685
                     /gene="feoA"
                     /locus_tag="OO135_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5396765.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            9702..12023
                     /gene="feoB"
                     /locus_tag="OO135_000823"
     CDS             9702..12023
                     /gene="feoB"
                     /locus_tag="OO135_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5396766.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGAHQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            12023..12259
                     /gene="feoC"
                     /locus_tag="OO135_000824"
     CDS             12023..12259
                     /gene="feoC"
                     /locus_tag="OO135_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5396767.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            12462..13340
                     /gene="rpnA"
                     /locus_tag="OO135_000825"
     CDS             12462..13340
                     /gene="rpnA"
                     /locus_tag="OO135_000825"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5396768.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTPNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(13369..14139)
                     /gene="bioH"
                     /locus_tag="OO135_000826"
     CDS             complement(13369..14139)
                     /gene="bioH"
                     /locus_tag="OO135_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5396769.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            14177..14860
                     /gene="gntX"
                     /locus_tag="OO135_000827"
                     /pseudo
     CDS             14177..14860
                     /gene="gntX"
                     /locus_tag="OO135_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
     gene            14919..15494
                     /gene="nfuA"
                     /locus_tag="OO135_000828"
     CDS             14919..15494
                     /gene="nfuA"
                     /locus_tag="OO135_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5396770.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            15855..17171
                     /gene="gntT"
                     /locus_tag="OO135_000829"
     CDS             15855..17171
                     /gene="gntT"
                     /locus_tag="OO135_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5396771.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQMLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(17216..19300)
                     /gene="malQ"
                     /locus_tag="OO135_000830"
     CDS             complement(17216..19300)
                     /gene="malQ"
                     /locus_tag="OO135_000830"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5396772.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(19310..21703)
                     /gene="malP"
                     /locus_tag="OO135_000831"
     CDS             complement(19310..21703)
                     /gene="malP"
                     /locus_tag="OO135_000831"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5396773.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLIGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            22315..25020
                     /gene="malT"
                     /locus_tag="OO135_000832"
     CDS             22315..25020
                     /gene="malT"
                     /locus_tag="OO135_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR5396774.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQTTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(25063..26079)
                     /gene="rtcA"
                     /locus_tag="OO135_000833"
     CDS             complement(25063..26079)
                     /gene="rtcA"
                     /locus_tag="OO135_000833"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5396775.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLANIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(26083..27309)
                     /gene="rtcB"
                     /locus_tag="OO135_000834"
     CDS             complement(26083..27309)
                     /gene="rtcB"
                     /locus_tag="OO135_000834"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5396776.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            27498..29096
                     /gene="rtcR"
                     /locus_tag="OO135_000835"
     CDS             27498..29096
                     /gene="rtcR"
                     /locus_tag="OO135_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5396777.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(29078..29836)
                     /locus_tag="OO135_000836"
     CDS             complement(29078..29836)
                     /locus_tag="OO135_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5396778.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(29853..30683)
                     /gene="glpG"
                     /locus_tag="OO135_000837"
     CDS             complement(29853..30683)
                     /gene="glpG"
                     /locus_tag="OO135_000837"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5396779.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(30728..31054)
                     /gene="glpE"
                     /locus_tag="OO135_000838"
     CDS             complement(30728..31054)
                     /gene="glpE"
                     /locus_tag="OO135_000838"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5396780.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            31244..32749
                     /gene="glpD"
                     /locus_tag="OO135_000839"
     CDS             31244..32749
                     /gene="glpD"
                     /locus_tag="OO135_000839"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5396781.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDTRLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(32801..33406)
                     /locus_tag="OO135_000840"
     CDS             complement(32801..33406)
                     /locus_tag="OO135_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396782.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(33409..34176)
                     /locus_tag="OO135_000841"
     CDS             complement(33409..34176)
                     /locus_tag="OO135_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396783.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLAGLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(34179..34889)
                     /locus_tag="OO135_000842"
     CDS             complement(34179..34889)
                     /locus_tag="OO135_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396784.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVVKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(34905..36410)
                     /locus_tag="OO135_000843"
                     /pseudo
     CDS             complement(34905..36410)
                     /locus_tag="OO135_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT         8890 a         9858 c         9132 g         8634 t
ORIGIN      
        1 tacagcgtcg tatccgataa aagcgttgtc acttacttaa gtttatttcc agcgtttcac
       61 catctaccgt cagatcgcgc cacgctttcg gcctcgtccc cagttttcca tccttcgcag
      121 ctgtgagtaa tttttctttc gttatttcgt tcggaagtaa actcatatca agcgcgtccc
      181 attcttttct gtctgagtca aagccataga caatgactct gtcatcatta aaagcaaaca
      241 ggatccgctc cgcctgacca tcactattca gatcttgctc gaccaggacg caggcgtctt
      301 tttcaataca ggtcatcacg ttatagcgat cctggattaa ggccgaccag aatgtcgcat
      361 ccggtttaac agaaccaggg gcaattaaca cattttcggc taatactttt tccgatacct
      421 gttcctgaag atgttgctct ccatctaacg ccatcagcag atcccgcgcg cgtttcgggt
      481 ctttcataaa cccggcatcg cttttcagcg actcaagcgc cgcacgtcca tagcgaccgc
      541 tctgttcgag catgtagata gttacctggt ctggcgtgtt tttgccgctc tgataacgcg
      601 ccatatgact gttcacgcta atacgcatac tgtccagcac cggcgaatta agaagcacca
      661 gtatcaccaa caccaataac gaaaccgcaa ggttcacttt gccctgaaga acaaggggat
      721 tttgcccatt acgccagacg atactgacaa aataccccag cgaccacacc agtaacacca
      781 gcaccgccag cgcgccctgc aagcggtcga cagtccagcc gtactgagcg acccgcagcc
      841 ataacgccca ggcggcgacg aacacataca gcggagcaac cagcaaagcg gttttaatca
      901 ggcaacgcaa cggccccgtc cagggaagtg acgctttttg cggatcgcgg acaatagcca
      961 ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc ggcggcggag atgtggcgag
     1021 aaatcgcgct caggcccgta aacggcagag tgatgataaa catcagggtt agcaatgata
     1081 ctaacggcag caaccccgtg gcgattaacg tgaacaactt ttgaatagag tcgattaaac
     1141 gtgactgtgt gcgcgcgaga atcaccgcca gtgcggtaac cagacctaat gtaagataca
     1201 taaaccagtc ggttgcaaaa aagagcgtat tgaaaaactt gatgccaaca agtttaaaca
     1261 actcacccca cagtaaaagc accagccacg tcaggccatt ggcgagaaaa atcaccagta
     1321 atataagtac gttatgccat actgactggt aaaagtagct atagcggcta ctgccattgc
     1381 ggatacgaag gctttgttgt atccacggta acaacaacat tgccatcaac agcagatagc
     1441 agccaaaatc ccaaatcgcc ttttcagctc tccaggggtt catgccatca gtttgccact
     1501 tcagccaacc gctcatccct gtcgtggcaa taaacaccag cgccagccat ccccaaaggc
     1561 gcttctgttt aaaagagatc acggagaaga gtaaaacgga tgaaaacgca acggtcgcag
     1621 gcacgccata aacaatccaa ctgtgatttt ttccggcaag ccagacgatc aacaggtagc
     1681 aaaccagccc ctgaagtaat ccggtagcaa tcatccccca gcgtgttgcc ggtgaaagtt
     1741 caacgttatc catgttggtt atccagaatc aaaaggtggg ttaattatcg catccgggca
     1801 gtagtatttt gcttttttca gaaaataatc aaaaaaagtt agcgtggtga atcgatactt
     1861 taccgattga atttgcatca atttcattaa ggaatgcgat tccactcaca atattcccgc
     1921 catataaacc aagatttaac cttttgagaa cattttccac acctaaaatg ctatttctgc
     1981 gataatagca accgtttcgt gacaggaatc acggagtttt ttgtcaaata tgaatttctc
     2041 cagatacgta aatctatgag ccttgtcgcg gttaacaccc ccaaaaagac tttactattc
     2101 aggcaataca tattggctaa ggagcagtga aatgcgcgtt aacaatggtt tgaccccgca
     2161 agaactcgag gcttatggta tcagtgacgt acatgatatc gtttacaacc caagctacga
     2221 cctgctgtat caggaagagc tcgatccgag cctgacaggt tatgagcgcg gggtgttaac
     2281 taatctgggt gccgttgccg tcgataccgg gatcttcacc ggtcgttcac caaaagataa
     2341 gtatatcgtc cgtgacgata ccactcgcga tactttctgg tgggcagaca aaggcaaagg
     2401 taagaacgac aacaaacctc tctctccgga aacctggcag catctgaaag gcctggtgac
     2461 caggcagctt tccggcaaac gtctgttcgt tgtcgacgct ttctgtggtg cgaacccgga
     2521 tactcgtctt tccgtccgtt tcatcaccga agtggcctgg caggcgcatt ttgtcaaaaa
     2581 catgtttatt cgcccgagcg atgaagagct ggcaggtttc aaaccagact ttatcgttat
     2641 gaacggcgcg aagtgcacta acccgcagtg gaaagaacag ggtctgaact ccgaaaactt
     2701 cgtggcgttt aacctgaccg agcgcatgca gctgattggc ggcacctggt acggcggcga
     2761 aatgaagaaa gggatgttct cgatgatgaa ctacctgctg ccgctgaaag gtatcgcttc
     2821 tatgcactgc tccgccaacg ttggcgagaa aggcgatgtt gcggtgttct tcggcctttc
     2881 cggcaccggt aaaaccaccc tttccaccga cccgaaacgc cgcctgattg gcgatgacga
     2941 acacggctgg gacgatgacg gcgtgtttaa tttcgaaggc ggctgctacg caaaaactat
     3001 caagctgtcg aaagaagcgg aacctgaaat ctacaacgct atccgtcgtg atgcgctgct
     3061 ggaaaacgtc accgtgcgtg aagatggcac tatcgacttt gatgatggtt caaaaaccga
     3121 gaacacccgc gtttcttatc caatctatca catcgataac atcgttaagc cggtttccaa
     3181 agcgggccac gcgactaagg ttatcttcct gactgctgat gctttcggcg tgttgccgcc
     3241 ggtttctcgc ctgactgccg atcaaaccca gtatcacttc ctctctggct tcaccgccaa
     3301 actggccggt actgagcgtg gcatcaccga accgacgcca accttctccg cttgcttcgg
     3361 cgcggcattc ctgtcgctgc acccgactca gtacgcagaa gtgctggtga aacgtatgca
     3421 ggcggcgggc gcgcaggctt atctggttaa cactggctgg aacggcacag gcaaacgtat
     3481 ctcgattaaa gatacccgcg ccattatcga cgccatcctc aacggttcgc tagataatgc
     3541 agaaaccttc actctgccga tgtttaacct ggcgatccca accgaactgc cgggcgtaga
     3601 cacgaagatt ctcgatccgc gtaacaccta cgcttctccg gaacagtggc aggaaaaagc
     3661 cgaaaccctg gcgaaactgt ttatcgacaa cttcgataaa tacaccgaca cccctgcggg
     3721 tgccgcgctg gtagcggctg gtccgaaact gtaatgattt gaagctggag aatatctatc
     3781 cagtatctta tagaaagcaa aacgggaggc accttcgcct cccgtttatt tacccttctt
     3841 ttgtcgtgcc ctgcgcccgc gttaccggca ctggcagcca ggcgcgaatg gaaagcccgc
     3901 cccgctcgct gctgccaagc tccagcatcc cgttatggtt atccacgata cgctgcacaa
     3961 ttgccagccc taatcccgtg ccgctaatgg tgcgtgcact gtcgccgcgg acaaacggct
     4021 ggaacaggtg cttacgttgt tccggcgcaa ttcccggacc gtcatcttcc acctggaacc
     4081 aggcgcgatt cggctccgtc ccgctgctga ctttgatcca gccattgccg taacgggcgg
     4141 cgttgaccac catattcgcc accgcgcgtt tgatcgacag cgggtgcatt ttcacttcaa
     4201 tgctgccggg gtaaagcgcg gtttcaattt cccgctcata gccactttcg gcagcaataa
     4261 cctcaccgag tactgcgttg agatccgcca tttccatcgg catctcctgc ccggtgcgca
     4321 ggtagtcgat aaactgctca atgatggcgt tgcactcttc gatatcttta ttgatcgatt
     4381 cggccaggta gccatcctgt tcgctcatca tctcggtcgc caggcgaata cgcgtcagcg
     4441 gcgtgcgtaa gtcatgactt acccccgcca tcagcagcgt gcggtcatcc gccagttgct
     4501 taacaccagc cgccatatgg ttaaaggcgc gggtaaccga acgtacctcg gacgcgccat
     4561 attcacgcag cggcggtgga ataatccctt taccgacctg caaagcagca tgttcgagat
     4621 cgaccaacgg tcggttctgg atacgaataa acagccacgc cccgcctatc gccaatagca
     4681 taatcgccag cgtatagcgg aacagcggag agaaatcgcc ctgatgaatt tcggtcagcg
     4741 gcacgcgtac ccagatattg ggcgacagcc aggttttcag ccagacgaca ggcgaacttt
     4801 tgttgacctc aacgcgcact tccgtcgggc cgcccagttg ctgtgccatc tgatggctta
     4861 agaattcata gtgttgcgcc caacgcagac ctgcctcttc ggcagcctcg ttggagtaga
     4921 gagagatccc cagctcacgg tagatctccc gacggaaagc gggaggcaca accaactgcg
     4981 tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg cacttcgtac gcgaggactt
     5041 tattaaactg ctggaggctc ggcaaaatcg cgaagttcag caccaccaga taagtcgtca
     5101 ccaggctggc gaacagcaag gtgacgatga gcaataacgt acgggcaaat gaacttcgtg
     5161 gcgagaagcg caatcgcctc atgctttaga gccgtccggt acaaagacgt agcccaggcc
     5221 ccaaacggtc tgaatgtaac gcggatgcgc tggatcttct tctaccatgc ggcgcagacg
     5281 cgaaatctgc acgtcgatgg agcgttccat tgcggaatat tcacgaccac gggcaaggtt
     5341 catcagctta tcgcgggaga gcggctcacg cggatggctg accagtgcct tcagtaccgc
     5401 aaactcaccg ctggtgagcg gcatcggctc atcttcgcgg aacatttcgc gcgtaccgag
     5461 gttaagtttg aacttaccga aagcaattac cgcctcttcc tgtgacggtg cgcctggcag
     5521 ttcgttcgcc tgacgacgca gcaccgcacg aatacgggcc agcagttcac gcgggttaaa
     5581 cggttttgga atgtagtcgt cagcgccaat ctccaggcct acgatacggt ccacttcttc
     5641 ccctttcgcc gtcaccataa tgatcggcat cgggttgctc tgactacgaa gacgtcggca
     5701 aatcgacaag ccatcttcac caggtaacat taaatccagt accataagat ggaaagattc
     5761 acgagtcagc aggcgatcca tctgttctgc attagcgacg cttcgaacct ggaagccttg
     5821 ttcggtgaga taacgttcca gcagcgcacg caggcgcatg tcgtcatcga ccaccagaat
     5881 cttgtagttc tcttgcattg tttgtactcc caaaggttcg caacaatttg taagcgtgta
     5941 ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt atgaatttca gcctaaattg
     6001 ttacaaagca tattaaacag cagcttaagt atacaattta ttcggcgaaa cattattgat
     6061 tctgttgata tgatcacgtt atacccaatg tgcgcattat caaacagaca aagggaatca
     6121 acgagatgaa aacgcccctg gttacccggg aagggtatga aaaactcaaa caagagctta
     6181 attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa ggtgacctgg gccgcaagtc
     6241 tgggcgaccg cagcgaaaat gctgactatc agtataataa aaagcgtctg cgtgaaatcg
     6301 accgtcgcgt gcgctatctc actaaatgca tggaaaatct caaaatcgtc gattactccc
     6361 ctcagcagga aggcaaagtc ttttttggcg cgtgggtgga gattgaaaac gacgatggcg
     6421 tgactcaccg tttccgtatt gtcggctacg atgaaatttt tggccgtaaa gattacatct
     6481 ctatcgattc cccgatggcc cgcgcattgc tgaaaaaaga agtcggcgat ctggcggtgg
     6541 tgaatacccc tgccggggaa gcgagctggt atgttaatgc tatcgagtac gtgaaaccgt
     6601 aaggaagagt cttaacctcc tgccgatggc tggcattttt gccagccagt ccgtataact
     6661 atcccctgat ttttgatccg aaaagatgaa ctcaaaccat gatgaatgat tcgttctgcc
     6721 gcattattgc gggtgaaatt caggcgcgcc cggaacaggt tgacgctgcc gttcgcctgc
     6781 ttgacgaagg gaataccgtg ccgtttatcg cacgttatcg taaggaaatc accggcggtc
     6841 tggatgacac gcagctgcgt aatctggaaa cgcgtctgag ctatctgcgc gagctggaag
     6901 agagacgtca ggcgatcctc aagtccattt ccgagcaagg caaactcacc gatgatctgg
     6961 cgaaggccat caacgccacc ctaagcaaaa ccgaactcga agacctctac ctgccctaca
     7021 aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc agggcttgag ccgttggctg
     7081 acctgctgtg gagcgatccg tcacacacgc cagaagtcgc cgctgcacaa tatgttgatg
     7141 ccgataaagg cgtggcagat accaaagccg cgctggatgg cgcgcgctat atcctgatgg
     7201 aacggtttgc cgaagatgcc gcgctgctgg cgaaagtgcg tgattatctg tggaagaacg
     7261 cgcatttggt ttctacggtg gtgagcagta aagaagagga aggggcgaaa ttccgcgact
     7321 atttcgatca tcacgaaccg ttgtccacgg tgccttctca ccgcgcgctg gcgatgttcc
     7381 gtggacgtaa cgaaggcgta ctccagcttt cgctgaatgc cgatccgcag ttcgatgagc
     7441 cgcccaaaga gagctattgc gagcaaatca tcatggatca cctttgcctg cgcctgaaca
     7501 atgccccggc ggatagctgg cgcaaaggcg tagtgagctg gacctggcgc atcaaggtgc
     7561 tgatgcatct ggaaaccgaa ctgatgggta ccgtgcgcga acgcgcggaa gatgaagcaa
     7621 tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc ggcccctgcc ggactgcgtg
     7681 caacgatggg cctcgatccg ggtctgcgta ctggggtaaa agtggcggtg gtcgatgcca
     7741 ctggcaaact ggtagcgacc gataccattt acccgcacac cggacaagcc gcaaaagcag
     7801 cgatgaccgt tgctgccttg tgtgaaaaac ataacgttga actggtggcg atcggcaacg
     7861 gtacagcttc ccgcgaaact gaacgtttct atctcgacgt gcagaagcag ttcccgaaag
     7921 tgaccgcaca gaaagtgatc gtcagcgaag ctggcgcgtc ggtttactca gcttccgagc
     7981 tggcagcgca ggagttcccg gatctcgacg tttcgctgcg tggcgcggtg tctatcgccc
     8041 gccgtttgca ggatccgctg gcggagctgg tgaaaatcga tccgaaatct atcggcgtag
     8101 gtcagtatca gcatgacgtc agccagacgc aactggcccg caaactggac gcagtagtag
     8161 aagactgcgt aaacgccgtt ggcgtcgatc tcaacactgc ttctgttccg ctgttaaccc
     8221 gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt ggcctggcgc gatgagaacg
     8281 gccagttcca gaaccgtcag caactgctga aagtcagccg tctggggccg aaagccttcg
     8341 agcagtgcgc gggcttcttg cgcattaacc acggtgataa cccgctggat gcttctaccg
     8401 tccacccgga agcctatccg gtggtggaac gcattctgac agcaacacag caggcactga
     8461 aagatctgat gggtaacagc agcgaactgc gtaacctgaa agcgtctgac tttactgatg
     8521 aaaaattcgg tgtgccgaca gtaactgaca tcatcaaaga gctggaaaaa ccgggtcgcg
     8581 atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg cgtcgagaca atgaacgacc
     8641 tgcaaccggg tatgatcctc gaaggtgcgg tgaccaacgt caccaacttt ggcgcgtttg
     8701 tcgatatcgg cgtgcatcag gacggcctgg ttcacatctc ttcattgtcg aacaagtttg
     8761 tggaagatcc gcataccgtg gtgaaagcgg gcgacattgt gaaggtgaaa gtgctggaag
     8821 tggatcttca gcgtaaacgt atcgccctga ctatgcgtct ggacgagcag cctggcgaaa
     8881 ccaacgcccg tcgcggcggc ggtaatgaac gcccgcaaaa caaccgcccg gcagccaaac
     8941 cacgcggtcg tgaagcgcag cctgccggta atagcgcgat gatggatgcg ctggcggcgg
     9001 caatgggcaa aaaacgttaa acgcccggac tggcccctaa acctgcgcga taaggcgttc
     9061 acgccgcatc tggcaacgaa cgccttgcct gacataaaag tgccggagaa tatctccggc
     9121 ctttttattc cacaaccaaa ctcataatat attccggcaa tatttatcac ttcattaaca
     9181 actgaaacct taattaaaca ttagccagtc cgggtaattc actattcgaa ttatattttc
     9241 gttgcgatat aaccttgagc cacatcaaca ttgagtcaga ttattattca aaccaacatt
     9301 cgcacacatt ttaagtattg ctgatagaaa ccattctcat tatcattgtg ttgttaatta
     9361 tttaatctct cctttgttgg caaatcatct ggtctcatgt cgctgtcaaa cgccccatga
     9421 ggtagttatc cagttaatga gaaacaagta ggcacctatg caatacactc cagatactgc
     9481 gtggaaaatc actggctttt cccgtgaaat cagcccggca tatcgccaaa aactgctttc
     9541 tcttggcatg ttacctggct cctcttttaa tgtggtgcgc gtcgctccac tcggcgaccc
     9601 cattcatatc gaaacccgtc gtgtgagcct ggtattacgc aaaaaagatc tggccttatt
     9661 agaagtggaa gcggtttcct gttaatacgg tgataacaac aatgaaaaaa ttaaccatag
     9721 gcttaattgg taatccaaat tctggcaaga caaccttatt taaccagctc actggcgcac
     9781 atcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg taaagaaggg caattctcca
     9841 ccaccgatca tcaggtcacg ctggtggacc tacccggcac ctattctctg accaccatct
     9901 catcgcagac ctcgctcgat gagcaaatcg cctgtcacta cattttgagt ggcgacgccg
     9961 acctgctgat taacgtggtg gatgcgtcta accttgagcg taacctgtac ctgacgctac
    10021 aactgctgga actcggcatt ccctgcattg tggcactgaa catgctcgac attgccgaga
    10081 agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg tctgggctgt ccggtgatcc
    10141 cgctggtttc aacccgtggt cgcggtattg aagcgctcaa gctggcgatt gatcgctata
    10201 aagctaacga gaatgtggaa ctggtgcatt acgcacagcc gctgctcaac gaagcagatt
    10261 cactggcaaa agtgatgcct tccgacatcc cgctgaaaca acgtcgctgg ctgggcctgc
    10321 aaatgctgga aggcgatatc tacagccgcg cctacgccgg tgaagcgtcg cagcatctgg
    10381 atgccgccct cgcccgtctg cgtaatgaga tggacgatcc ggcgctgcac attgccgatg
    10441 cgcgttacca gtgcattgct gccatctgtg atgtggtaag caacaccctg acggcagaac
    10501 ccagccgttt caccactgcg gtagataaaa tcgtgctcaa ccgtttcctc ggtctgccga
    10561 ttttcctctt tgtgatgtac ctgatgttcc tgctggctat caacatcggc ggggcgttac
    10621 agccgctgtt tgacgtcggc tccgtggcgc tatttgtgca tggtattcaa tggattggct
    10681 acacgctcca cttcccggac tggctgacta tcttcctcgc ccagggcctg ggtggcggca
    10741 ttaacaccgt gctgccactg gtgccgcaga ttggcatgat gtacctgttc ctctccttcc
    10801 ttgaggactc ggggtatatg gcgcgtgcgg cgtttgtgat ggaccgtctg atgcaggcgc
    10861 tgggcttgcc ggggaaatcc tttgtgccgc tgatcgtcgg tttcggttgt aacgtaccgt
    10921 cggtaatggg tgcacgtacg cttgatgcac cgcgtgaacg tctgatgacc atcatgatgg
    10981 caccgtttat gtcctgcggc gcgcgtctgg ctatcttcgc agtattcgcg gctgccttct
    11041 tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat gctgggtatt gtgatggcgg
    11101 tgctgactgg cctgatgctc aagtacacca tcatgcgcgg tgaagcgacg ccgtttgtca
    11161 tggagttgcc ggtctatcat gtaccacacg ttaaaagcct gattatccag acctggcagc
    11221 gtctgaaagg cttcgttctg cgtgctggta aagtgatcat catcgtcagc attttcctga
    11281 gcgctttcaa cagcttctcg ctgagcggga aaatcgtcga taacatcaac gactcggcgc
    11341 tggcgtccgt cagccgggtg atcaccccgg tcttcaagcc aattggcgtg catgaagata
    11401 actggcaggc aacggttggc ctgtttacag gtgccatggc gaaagaagtg gtagtgggta
    11461 cgctcaacac cctctacacc gcagaaaata ttcaggacga agagttcaat ccggcagagt
    11521 ttaacctcgg tgaagagctg ttcagtgcga tagatgaaac ctggcagagc ctgaaagaca
    11581 ccttcagcct tagcgtactg atgaacccca ttgaagccag caaaggcgac ggcgaaatgg
    11641 gtaccggggc gatgggcgtg atggatcaga aattcggtag cgcagcagca gcttacagct
    11701 acctgatttt cgtcctgctg tatgtaccat gtatctcggt gatgggagcc atcgcccgtg
    11761 aatcaagccg tggctggatg ggcttctcca tcctgtgggg gctgaatatc gcttactcgc
    11821 tggcaacatt gttctatcag gtcgccagct acagtcagca cccaacttac agcctggtgt
    11881 gcattctggc ggttatcctg tttaacatcg tggttatcgg tctgctgcgc cgcgcgcgta
    11941 gccgggtgga tatcgaactg ctggcaaccc gcaagtcggt aagcagttgc tgcgcagcca
    12001 gcaccaccgg tgattgccat taatggcttc acttattcag gtgcgcgatt tgctggcgtt
    12061 acggggccgt atggaagcgg cccagataag ccagacattg aacactccac agccaatgat
    12121 taacgccatg ctgcaacaac tggaaagtat gggcaaagcc gtgcggattc aggaagaacc
    12181 tgacggctgc ctctctggca gttgtaaaag ctgcccggaa ggaaaagcct gtctgcgcga
    12241 gtggtgggcg cttcgttaac cttactccat cgcctgtttt ggaaagcccg gtatgcgtct
    12301 gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc aactcttgca gcacggcgta
    12361 agttcctttg aaagcatctc gcagggatga aaactcgcta atacacaggt gtggagtggc
    12421 gcgtagagtc gcggcattca aacaacaggt gaaggaacgc catgagcaaa aagcagagtt
    12481 ccaccccaca cgatgcgctg ttcaaactct ttttacgcca accggacacg gctcgtgatt
    12541 ttcttgcgtt tcatttaccg gcacccattc acgcgctttg tgatatgaaa accctcaagc
    12601 tggagtcgag cagctttatt gatgacgatc tgcgtgaaag ctattccgat gtgctgtggt
    12661 cggtgaaaac ggaacaagga ccaggataca tctattgtct gattgaacat caaagcaccc
    12721 caaacaaact gatcgcattt cgcatgatgc gttacgctat tgccgcaatg caaaatcacc
    12781 ttgatgctgg atacaaaacg ttgccgatgg tggtgccatt gttgttttac cacggtattg
    12841 aaagccccta tccctattcg ctgtgttggc tggattgttt cgccgatccc aaactggcaa
    12901 ggcagcttta tgcctccgca tttccgctga ttgatgtcac cgtcatgcct gatgatgaaa
    12961 tcatgcagca ccgacgtatg gcgctgctgg agttaattca aaaacatatt cgtcaacgcg
    13021 acctgatggg gctagtagag caaatggcct gcttattaag tagtggatac gctaatgaca
    13081 gacaaatcaa agggctgttt aattacatac tgcaaaccgg cgacgcggta cgttttaacg
    13141 attttatcga cggcgttgcc gaacgttcac cgaaacacaa ggagagttta atgacaattg
    13201 cggaaagatt gcggcaggag ggggaacaat ccaaagccct gcatatagcc aaaataatgc
    13261 ttgaatccgg agtccctctt acagacatca tgcgctttac cggggtgtca gaagaagagt
    13321 tggctgcggc gagccagtaa atttctgtct cgccatttca aaagttgcct acaccctctg
    13381 cttcaacgcc accagcaggt gacaaaactc gaccggatgc gaaataaacg gcgcatgggc
    13441 cgctttggcg aagatatatg atttgctgcg aggccagagt ttatccagca tcggcaccac
    13501 tttgcgcggc accagaccat cgagatagcc atacaatcgc aaaaacggca tgggcacgtt
    13561 ttgcagcggc tgacggagat caaccgtttt caggatttcc agcccgccat taagcacgtc
    13621 aacctccggc atcggtaacg ccagaacggt tttcttcaac gcccgcgcat cctggcgcgc
    13681 cgtttcagtc cccatggttt gtaacgccag gaaccgctcc actgtacgct gaaaatcatc
    13741 actgagttgc tgctgaaatc ccgccagcac gtccggtttt atccccggcc aatcgtcacg
    13801 agcactaaaa caaggtgacg acgccacggt gaccagcgcc tgaacacgct cgggatgggt
    13861 taacgcaatc tggcttgcca ccagcccgcc cagactccag cctaaccaaa tggctttatc
    13921 aggtgcctgt tgcagcacgg cttcggccat atcagcaagt gacagcgcac caaatccctg
    13981 gctgcgcccg aagccgggca gatcaacaag gtgcagcgta aaatgcgagc taagttcctc
    14041 gtcaatgcaa cgccacactt cggcattcag tccccatccg tgcagcagca caagatgaac
    14101 attcccctga cctttggtct gccaccagat gttattcatc cgctattgtt ctcttttgac
    14161 ttacaaggat gaacatatgc taacagtacc gggattatgc tggctatgcc gaatgccgct
    14221 ggcgttaggt cattgaggga tttgttcggt ctgctcacgc gccgcccgta cagataaaac
    14281 gttatgccca caatgtggat tacccgccac acactcacat cttccctgcg gtcgctgcct
    14341 gcaaaaacca ccgccctggc aaagactggt cacggttgcc gactatgcgc cgccgttaag
    14401 tccgcttatc caccagctta aattttcccg gcgcagtgaa atcgccagcg ccctgtcacg
    14461 tctgttacta ttggaagtct tacacgctcg tcgcaccacc gggttgcaat tgccggatcg
    14521 catcatcagc gttccgttat ggcagcggcg tcactggcgt cggggattta atcagagcga
    14581 tttgctgtgt cagccgttat cacgctggtt gcactgccga tgggatagcg aagccgtcac
    14641 acgaacacgg gccactgcga cccagcattt tctcagtgcc cggctacgca agcgcaacct
    14701 gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc catatggtga ttgtggatga
    14761 tgtcgttacc accggaagta ccgtcgcaga gattgcgcag ttgcttttac gcaatggtgc
    14821 ggcgactgtc caggtctggt gcctttgtcg aaccttgtag agcctcgatg atgggcgtat
    14881 tataaccaac taaaatagtc aactattagg ccattactat gatccgtatt tccgatgctg
    14941 cacaagcgca ctttgccaaa ctgctggcaa accaggaaga agggacacaa atccgcgtat
    15001 ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt ttcttattgt ccgccggatg
    15061 ccgtggaagc caccgacact gccctgaaat ttgacctgct gaccgcgtat gttgatgagt
    15121 taagcgctcc ttacctggaa gatgcagaga tcgattttgt taccgaccag ttgggttctc
    15181 agctaacact gaaagcgcct aacgccaaaa tgcgtaaagt ggcagacgat gcaccactga
    15241 tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc acagcttgct ggtcacggtg
    15301 gccgcgtttc actgatggaa atcaccgaag acggttacgc cattctgcaa tttggcggcg
    15361 gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga agggatcgag aagcagctgc
    15421 taaacgaatt cccggagctg aaaggtgtgc gcgatctcac cgaacaccag cgcggcgaac
    15481 actcctacta ctaagttatc ccctcttttg tagattgccc gatgcgacgc taaagcgtca
    15541 tttcgggcct acgttgaaca tgtgccagac gttggcgcag cattgctttg gcaaatccct
    15601 cccttcgttt ctccgcgata atatgaccaa cctctcataa tttaaattta ctccgctctg
    15661 gtgattctca aacgccagat gttacccgta tcattcacat gggtaccaaa catactcctg
    15721 acatctgact acaataatta gttttagtgg gtatcagtcg tggtgccgca atatctctgt
    15781 tcccgattgg gataattaga gtttgtcgtc agaaaattga cgttacccat aacaaatgaa
    15841 aggccaggta aatcatgcca ttagtcattg ttgctatcgg tgtaatcttg ttgttgctcc
    15901 tgatgatccg tttcaaaatg aacggcttca tcgctctcgt cctcgtggcg cttgctgttg
    15961 gattaatgca agggatgccg ctggataaag ttattggctc catcaaagcc ggtgtcggcg
    16021 ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc gatgctgggc aaaatgctgg
    16081 cagactgcgg tggcgcacaa cgtatcgcca ccaccctgat tgccaaattt ggtaaaaaac
    16141 acatccagtg ggcggtggta ctgaccggtt ttaccgttgg ttttgccctg ttctatgaag
    16201 tgggctttgt gctgatgctg ccgctggtgt ttaccatcgc ggcttctgcg aatattccac
    16261 tgctgtatgt tggtgtacca atggcagctg cactgtctgt gacccacggc ttcctgccac
    16321 cgcatccggg tccgactgcg attgccacca ttttcaatgc cgacatgggt aaaaccctgc
    16381 tgtacggtac tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg
    16441 tgttgaaagg tatcgataag ccgatccctg aaggtctgta cagcgcgaaa accttcagcg
    16501 aagaagagat gccgagcttt ggcgtcagcg tctggacctc tctggtgccg gtagtgctga
    16561 tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg tcatgctttc ctgccggtag
    16621 cggagttcct cggtgacccg gtaatggcta cgctgattgc cgtgctgatt gcgatgttca
    16681 cctttggtct gaaccgtggt cgttcaatgg atcagattaa cgacacgctg gtttcttcca
    16741 tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg cggtgcgttc aagcagatgt
    16801 tggtagacag cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc
    16861 cgctgctgat ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg
    16921 ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc
    16981 cggagctgat ggttattgcg gtgggttccg gtagtgtgat tttctctcat gtgaacgatc
    17041 cgggcttctg gctgttcaaa gagtacttta acctaactat cggcgagacc atcaaatcct
    17101 ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata
    17161 tggtgatttg ataacactga ctgccggatg catcgcatcc ggcatcaacc gcactctact
    17221 gcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt cagcaactta ttcacgccat
    17281 catcggcaaa catcgtctca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt
    17341 aactggtgcc aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc
    17401 ctaacagagc actgttactg tcggcaatgt agcgctgcaa accacgatta agcgtcggcg
    17461 tcatcgacat caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca
    17521 gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca
    17581 cttcatccgg atacagcccc agggttttgc ccaacgttag atccccgctc tcccagtaac
    17641 cgcgcagcgt aggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt
    17701 tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taggagtaca
    17761 caccgctgct gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa
    17821 tcaccataca gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg
    17881 gatagtgaac atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca
    17941 gcatcgacat cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca
    18001 gctcgataaa cggttcatag gcacgcgcgg tgatgatatg cgggtccatt ggcggtaatc
    18061 cccagttctg ccccaacggg ccgaggatat ccggcggcgc gccaaccgac gctttcaggc
    18121 aatatagttc acggtcacac caggtttccg ccccaccttc cgctacgcca accgccagat
    18181 cacgatacaa gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact
    18241 ggctgtaagc cagccactgc aaccagagat aaaaatcgac gtcatcacga tgttcttcgc
    18301 agaactgacg cacttctggt gaatccacgt tctgatacat ctctggccat gcaggccagc
    18361 cccagcgcat ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc
    18421 agaacaggct gtcgccctgc tctgcaacaa actggcgaaa cgcggtcatc tgctcatcat
    18481 cacgttgcgc gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg
    18541 tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag cgtctgttgc gtggtcggca
    18601 actgccacca ggcctgagcc tcttcgctaa gatggaaatc ttcaacggcg ttaacgtcga
    18661 tataaatcac attcagccaa cggcgagaag acgggctgta tgggctggca ctctccggat
    18721 ttgccggata gagcgcatga atcgggttca ggccgatgaa cgacccgcca cgttttgcca
    18781 catccaccag catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc
    18841 gcagcgtata aagctgaacg caggcacccc acagcttttg tttattcagc aacgcctgcg
    18901 gttcgtaaca gcgtttcggg gcgacaatca cccggcaatg cgcgcgctgg tcgtcctggg
    18961 tgagtgtcag cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc
    19021 ccgttacatg gcctttgtac tgtgttcctt cttcggtggt cagcagccag ctatattcgc
    19081 cgctgccctc caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga
    19141 ctggcgttac cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt
    19201 tggtttcggc gctaatcgac tgcggtttac cgtgggcatt gatgtaattg gggctaatcc
    19261 ccgccgccag cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg
    19321 cctgccagat acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc
    19381 gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca
    19441 cctgcttttg tgcctcaaca taggctgcga aatccgccat caccagatac ggatcgccgc
    19501 cttgtttgcc gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt
    19561 taccgctttc cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca
    19621 ccgggtcgta gcctttggcc agaattgcct tcacttgttc cacggtatga ccaaaaataa
    19681 agatattttc ttcaccgact ttctcggcga tttcaacgtt cgcaccatcc agcgtaccga
    19741 cagtaagcgc accattgagc gccagtttca tattgccggt accggaagct tctttacctg
    19801 cagtcgaaat ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc
    19861 aataatccgg caggaacacc accttcaact tatcgccaat cagtggatcg ttgttgatca
    19921 cgtcagccac tttgttgatc gcaaagataa tattcttcgc caggtagtag cccggtgccg
    19981 ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc ctgcgggttt tcacgaattt
    20041 ctttgtacaa cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac
    20101 gtttaatctg aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca
    20161 caaactccgc cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt
    20221 tcgcatcatc agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt
    20281 tttgcagtaa tttatccaac agagccgcca gtgcagggtt gcactgtttg atccagcgac
    20341 gtggggtaat accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg
    20401 ggaacagatc tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac
    20461 cgccaaccac acacaggttc gccatatgca cttgtttatc gtgcacaacc gccagtttgg
    20521 cccacacttt ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa
    20581 tttcgttaat aatctgcata tggcgcggca gtaagccttt caccagtttc acatcccagc
    20641 gttccagcgc ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtaatgg
    20701 cccaggcgtc atcccagctc atctggtgct catcgatcag cacgcgcagc agttctggaa
    20761 tcgcgatagt tgggtgggta tcgttcagct gaataacttc ataatccgcc agttcgtgca
    20821 gtttacgccc cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga
    20881 agtactgctg catcaagcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa
    20941 ctttggtcag tttttccgca ttgatgccct gctgttcggc acgcaggaaa tcaccgtcgt
    21001 taaatttagt cagatcaaac ggatgcgcgt gcgtcgcctg ccacagacgc agcggctgcg
    21061 ccacgccatt acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact
    21121 ccggctccca gcgtccgtct ttcgtcactt taccgccaat ccctacctgc acatccagtg
    21181 cttcgttgtg gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct
    21241 gtttgccatc gacaaaagac tggcggaaca aaccatattg atagttcaga ccgtaacccg
    21301 tcgcagactg accgatagtt gccattgagt cgaggaagca cgccgccaga cgtcccagac
    21361 caccgttacc cagcgccggg tcgatctctt cttccagcag gtccgtcaga ttgatgtcat
    21421 aagccttcaa cgaatcctgt acatcctgat accagccgag attcaacagg ttgttgcccg
    21481 tcaggcgacc aatcaaaaac tccattgaga tgtagttaac atgtcgctga ttcgccaccg
    21541 gcttggcgaa tggctgagca cgcagcattt cggccagtgc ttcactcact gctagccacc
    21601 actggcgagg agtcatttca gccgcagaat ttaagccata acgctgccac tgacgtgaaa
    21661 gcgcttcctg aaattgctta tcgttaaaaa taggttgtga cataggagtt ccacttttct
    21721 tagattttca acacaacgtt atcgctagtt tgccaggctc gatgttgacc ttcctcatcc
    21781 tgcgggggat taggcaggga ggagttgcgg ggatgagcaa ggaaatgtga tctcaaccac
    21841 ttaaagctag tgcaaaccac aggattagca gcaaatcaat gcaatacagc gcagaaaatc
    21901 tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa aagcggaagg taactctata
    21961 aattaagtaa aggagtgaaa cagtttcaca agtaaaatat ccagtgtgct ccatctcatt
    22021 cttaatagat ttattaagat catcttttta gatggcactt tcatcaggaa tgaagaagaa
    22081 acccttgctt aaatgaatct gatgaacata agggaaacca gtattcacgc tggatcagcg
    22141 tcgttttagg tgagttgtta ataaagattt ggaattgtga cacagtgcaa attcagacac
    22201 ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc cgaccttata accattaatt
    22261 acgaagcgca aaaaaaataa tatttcctca ttttccacag tgaagtgatt aactatgctg
    22321 attccgtcaa aactaagtcg tccggttcga ctcgaccata ccgtggttcg tgagcgcctg
    22381 ctggctaaac tttccggcgc gaacaacttc cggctggcgc tgatcacgag tcctgcgggc
    22441 tacggaaaga ccaccctcat ttcccagtgg gcggcaggca aaaacgatat cggctggtac
    22501 tcgctggatg aaggtgataa ccagcaagag cgtttcgcca gctatctcat tgccaccgtg
    22561 cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat
    22621 gccagcctga cgtcactctt cgcccagctt ttcattgagc tggcggaatg gcatagccca
    22681 ctttatctgg tcatcgatga ctatcatctg atcactaatc cagtgatcca cgagtcaatg
    22741 cgcttcttta ttcgccatca accagaaaat ctcaccctgg tggtgttgtc acgcaacctt
    22801 ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac tgctggaaat tggcagtcag
    22861 caactggcat ttacccatca ggaagcgaag cagttttttg attgccgtct gtcatcgccg
    22921 attgaagccg cagaaagcag tcggatttgc gatgacgttt ccggttgggc gacggcacta
    22981 cagctaatcg ccctctccgc ccggcagaat acccactcag cccataagtc ggcacgccgc
    23041 ctggcgggaa tcaatgccag ccatctttcg gattatctgg tcgatgaggt tttggataac
    23101 gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat
    23161 gcgctcatca cccgtgtgac cggcgaagaa aacgggcaaa tgcgtctcga agagattgag
    23221 cgtcaggggc tgtttttaca gcggatggat gataccggcg agtggttctg ctatcacccg
    23281 ctgtttggca acttcctgcg ccagcgctgc cagtgggaac tggcggcgga gctgccggaa
    23341 atccaccgtg ccgccgcaga aagctggatg gcccagggat ttcccagcga agcgattcat
    23401 catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg
    23461 agtttgttca accatagcga actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg
    23521 gacagtttgc tggaaaatcc gcagttagtg ttattgcagg cgtggctaat gcaaagccaa
    23581 catcgctatg gcgaagttaa caccctgcta gcccgtgctg aacatgaaat caaggacatc
    23641 agagaaggca ccatgcacgc agaatttaac gctctgcgcg cccaggtggc gattaacgat
    23701 ggtaatccgg atgaagcgga acggctggca aaactggcac tggaagagct gccaccgggc
    23761 tggttctata gccgcattgt ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc
    23821 gaattgaccc gctcactggc gctaatgcag caaaccgaac agatggcacg ccagcacgac
    23881 gtctggcact acgccttatg gagtttaatc cagcaaagtg aaattctgtt tgcccaaggg
    23941 ttcctgcaaa ccgcgtggga aacacaggaa aaagcgttcc agctgatcaa cgagcagcat
    24001 ctggaacagc tgccgatgca tgagtttctg gtgcgcatcc gagcacaact gttatgggcc
    24061 tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct
    24121 tatcagccac agcaacagct tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt
    24181 ggtgatttag ataacgcccg tagccagctg aaccgtctgg aaaacctgct ggggaatggc
    24241 aaatatcaca gcgactggat ctctaacgcc aacaaagtcc gggtgattta ctggcaaacg
    24301 accggcgata aagccgccgc tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg
    24361 aacaaccact tcctgcaagg tcaatggcgc aacattgccc gcgcacaaat cttgctgggc
    24421 gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg
    24481 ttgatgagcg atctcaaccg taacctgttg ctgcttaatc aactgtactg gcaggccgga
    24541 cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga
    24601 tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt
    24661 caactcaata cgctgccgga actggaacag catcgcgcgc agcgtattct gcgagaaatc
    24721 aatcaacatc atcggcataa attcgcccat ttcgatgaga atttcgttga acgtctgcta
    24781 aatcatccgg aagtgccaga actgatccgc accagcccgt tgacccaacg tgaatggcag
    24841 gtcctggggc tgatctactc cggttacagc aatgagcaaa ttgcgggcga gctggaagtg
    24901 gcggcaacca ccatcaagac gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac
    24961 cgtcaggccg cggtacaaca cgcgcagaaa ctgctgaaga tgatgggata tggggtgtga
    25021 gtttagccgg ataatgcatt ggatccggtt tacatcttta catcattcaa tactcacccg
    25081 cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc aagaaacgct ccaccacggc
    25141 gatattggcc agcagatggc atgaaggatg ggcgaccgta aattctcccg cgcccgccag
    25201 cgccatcggt agcaccagct ggtcggcgag atattccccc actgccgccg ggcttgccag
    25261 gtagcgtttc acctctttca ctaactgcgc ggctaccacc tcggcactga cgcgcttttc
    25321 accgacgacg aaaaagcgtt cagtgatatt ttcactttcg acttccaacg agacggtatt
    25381 acccggcccc tggtcgcgcg gcaggttatg aatattctgt tcatgcaggg aaaaactccc
    25441 cgccagtgta gcgatttcac gctcagcaac atggcgcggc acaccagcta atagaacttc
    25501 tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc aaggtgttaa acgatgccac
    25561 gggcgagact tccgttgcca ccacgccgcc tccggcagga taaaaaccgt gacgtaatag
    25621 cgtggtttgc tgatgaattc ctattttcgc cagcagcggc tccagcaccc ggcggataaa
    25681 atccgcaggc ggcgccgacg ggttatcggt gcctccgctc acttcaacac gcgaaggtcc
    25741 atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc agcgtacagc ttccggcact
    25801 accgatagca aagcggtact cgccgccgcg cacggcgcca ggccggaaga ccagacgctg
    25861 cgaccccagc tccgcgcctt ccaccgttgc cctacaaatt tccgcagccg cttttaccgc
    25921 ggtcagatgc tggcgcaaca gccccggttt cgcccgcccg gcacgaatgc tggtgatggt
    25981 aaatggctgg ccggttatca tcgacaggct cagcgccgag cgcagaatct gcccgccacc
    26041 ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc atttatcctt ttacgcacac
    26101 cacctgacgc agggtataga taacttccac cagatcgctt tgtgccgtca tcaccgcatc
    26161 aatatcttta tacgccatcg ggatttcgtc gatcacttcg gcatctttac ggcattccac
    26221 atgcgcggtg gcacgaattt gatcttccac gctgaacagt tttttcgctt tagttcggct
    26281 cattacccgc ccggcaccgt ggctgcacga acagaacgac tcttcatttc ccagcccacg
    26341 gacgataaag ctttttgctc ccatcgaacc gggaataatt ccatattgac cagcacgcgc
    26401 agacaccgcg cctttacgcg tcacgtagat ctcttcacca aagtgctgtt ctttttgcac
    26461 atagttgtgg tgacagttga tctcttccat cgccagcgtt tgtggctgtc tgaccgtttt
    26521 ctgcgtaatg ctctgcaatg ccgttaccac gttttccatc atcgcatcgc ggttaaggct
    26581 ggcaaaaagc tgcgcccagg ccacggcttt caggtaatca tcaaagtatt ccgtaccttc
    26641 cataaagtac gccagatcac gcgacggcaa cgtctcaagc gtttcctgca tctctttttg
    26701 tgccagatcg ataaagtaag tcccgatggc gttaccaatt ccgcgtgaac cggagtgcag
    26761 cataatccac acctggtccg actcatcaag gcagatttta ataaagtggt taccggttcc
    26821 cagcgttccc aggtgtttat agttattggt attcaggaaa cggggatatt tttgcgttaa
    26881 ccactgataa ccggcttcaa gctcagccca tttcgcatcg acgttaacag gcgggttttc
    26941 ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc ccgtgcggca cggccgtttc
    27001 aatcgcctga cgcagctctg ccaggttttc aggcaggtct tccgccgtta acgcggtacg
    27061 cagcgcgttc attccacagc caatatccac gcccaccgcc gccggaataa tcgccccttt
    27121 ggtcgggatc acgctaccaa tggtggaatc tttacccagg tgtacatcag gcattaccgc
    27181 aatatgtttg aaaataaacg gcatcttcgc cgtattaata agttgctgac gcgcatcggc
    27241 ttctaccggc acgcctttgg tccacatttt taccggggca ttttcagtgg tcagtaattc
    27301 gtaattcatt ttgtttttct cttttcgttg tttgctgtcc tgataattgc aaccgtcgtg
    27361 ccagaaaatt aaataagtaa attaattttt taattcattg tttttaaaaa gattatatct
    27421 ttatgtccgt aaccggagat ttcccgcaaa gccgatttac cgataatgaa atatcgtctt
    27481 ttataaggat atctaagatg cgtaaaacag tagcttttgg ctttgtcgga acggtgctgg
    27541 attatgccgg gcgcggcagt cagcgctggt caaaatggcg tccgacactc tgtttatgcc
    27601 agcaagaatc gttggtcatc gatcgactgg aattattgca tgacgcccgc tcgtgctcgc
    27661 tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc agaaacagaa gtggtgagca
    27721 ttgagattga actgcataac ccgtgggatt tcgaagaggt ttacgcctgc ctgcatgatt
    27781 tcgcccgtgg ttacaagttt cagccagaaa aagaagacta tttaattcac atcaccaccg
    27841 gcacccacgt cgcgcagatt tgctggtttc tgctggcgga agcgcgttat ctacccgccc
    27901 ggttgataca aacttcacca ccgcgcaaaa aagaacaacc gcgcggtgcc ggtgaagtga
    27961 cgattatcga tctcgatcta agccgttata acgccatcgc cagccgcttt gccgaggaac
    28021 gccagcaaac gcttgatttt cttaagtccg gcattgccac gcgtaacccc cacttcaacc
    28081 gcatgattga gcagattgaa aaagtggcga ttaaatcccg cgcaccgatc ctgcttaacg
    28141 gcccaaccgg tgcgggaaag tcatttctgg cgcgacgtat atttgaatta aaacaggcgc
    28201 ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc caccctgcgc ggcgataccg
    28261 ccatgtcgac gctgtttggt catgtgaaag gcgcgtttac cggggcgcgg gaatctcgtg
    28321 aaggtttatt acgcagcgcc aacggcggaa tgttgttttt tgatgagatt ggcgaactgg
    28381 gcgcagacga acaggcaatg ctgctaaaag ccattgaaga gaaaaccttt tacccgtttg
    28441 gcagcgatcg ccaggtgagc agtgattttc agcttatcgc cggaacggtg cgcgatttgc
    28501 gccagctggt tgccgaaggc aaatttcgcg aagatctgta cgcgcggatc aatctctgga
    28561 ccttcaccct gccgggtcta cgccagcgcc aggaagatat tgaaccgaac cttgattatg
    28621 aagtggagcg ccacgccaca ctcactggcg acagcgtgcg ttttaacacc gaagcgcggc
    28681 gcgcctggtt ggcttttgcg acctctcccc aggcaacatg gcgcggtaac tttcgcgaac
    28741 tttctgccag cgtcacgcgg atggccacct ttgccactag cggacgcatc actctggacg
    28801 tggttgaaga tgagataaac cgtctgcgct ataactggca ggagagccgc ccctccgcgc
    28861 ttacggcgtt gctgggcgca gaggcagaaa acatcgatct cttcgaccgt atgcaactgg
    28921 aacacgttat cgctatctgc cgccaggcaa agtcgctttc tgcagccgga cgacagcttt
    28981 ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc ggatcggcta cgcaaatacc
    29041 tggcgcgttt tggtctgacg tgggaagccg tgcaggatca gcacagctcc agttgaatat
    29101 gatggtccgt cagcacctgc attacgctta ctggcggcgg ggcgtcggtg tagacggcat
    29161 ctaccatgct gatgctgccc atattgacca tcgcgttacg gccaaatttc gagtgatcga
    29221 caaccagcat aacgtggcgc gagttctcaa taatggcgcg cttggtgcga acttcgtggt
    29281 aatcgaactc cagcagcgag ccgtcgctat cgatgccgct tatccccaga atgccgaaat
    29341 caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc aatgatcccg ccatcgcggc
    29401 tgcgtaattc gccaccggcg agaataatgc ggaaatcttc ttttaccatc aacgtgttag
    29461 caacattgag attgttggtg acaatacgca aattgctgtg attaagcagc gcgtgcgcca
    29521 ccgcttccgg cgtggtgccg atatcgataa acagtgtcga gccgttgggg atttgctccg
    29581 ccactttgcg ggcaatcctc tctttttctt cggtctgggt ggctttgcga tcgtgccacg
    29641 gcgtgttaac cgaactggaa ggcagcgccg caccgccatg gtgacgcagg atcaggtttt
    29701 gctccgccaa ctcattgagg tcgcggcgaa tagtctgcgg gctgacggag aaatgctcta
    29761 ccagctcttc agtactgaca taaccctgct gtttaaccag ttcgataata ccgttgtgac
    29821 gttgtgtttg tttcatttat aaatccctgg aattattttc gttttcgcgc attgagcgaa
    29881 tcaacaaaag ccatcgctaa acccacggct aacccggcga tgtgtgctcc gttcgccatc
    29941 gacatcccaa acaaatcaaa ccatccggcg acaatccaga tcagcgcaaa gataattaac
    30001 ccacgttgca ggtaaatgcc actttgcgga tcgcgctcac cacgtagcca gacgtagccc
    30061 atcagcgcat acaccacgcc agaaagcccg ccaaaccacg gcccgctgaa tttttgctgc
    30121 acatagccgc ttaacagggc gctaatgaga gtaataacaa ttagcttacc gctaccgagg
    30181 cgtttttcca ccgcaccgcc gagataccac caccagagca ggttaaagag gatatgcatc
    30241 agcgagaagt gcattaacgc gtgggtgaag taacgccaga actcaaattt cagtgttgga
    30301 tcgaatggcc aggccagcca taacatcact tcctgatcgc cgagaatttg catggcgata
    30361 aacaccacca cgcaggcgat catcaccacc caggttaccg gacctgcgcg ttcacgcaag
    30421 gcagcaaaga aaggataacg gcgataatgc agaccactgc cggtatggcc cgcctgccag
    30481 ctggccgcca gataacgcgg atctgccggg ttttcgagaa aacgcgccag ctccgcccgc
    30541 acgcgctcgg cctgggactc atccgccagc cagacatcgc tttggttatg ttgttgaatc
    30601 gtaaggataa caccctgcgt cgccatgtaa tcaacaaacg cctgcgccac gcgggggtta
    30661 gcaaaagagg taatcatcaa catcgttgct gtcgcttatt ccacacaaaa ggggacagta
    30721 taaagcgtta cgcgccgtac gccacctctg cgggaaactg acgctgccag gcttcaaaac
    30781 cgccgtcaat gctatagacc acatcgtagc cctgttgcag cagatactgc gccgcgcctt
    30841 tgctgctatt gccgtgataa cacatcacca tcaccggagt gtcaaagtcg ttatcacgca
    30901 taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg caccgcatgt cccattgcga
    30961 aactctgtgg atcgcgaata tcgaccagca ccgcctcttt ttcctgcaac ttctggtgcg
    31021 cgtcggcaac gttaatacat tcgaacagat ccatgcgtct ctctttcttt acaaacaagt
    31081 gggcaaattt accgcacagt ttacgtcgaa gcggcagata aacgccataa tgttatacat
    31141 atcactctaa aatatttttt caatgttacc taaagcgcga ttctttgcta atatgttcga
    31201 tagcgaacat ttatgagctt taacgaaagt gaatgagggc agcatggaaa ccaaagatct
    31261 gattgtgata ggtggcggca tcaatggtgc tggtatcgcg gcagacgccg ctggacgcgg
    31321 tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc gcgacctctt ccgccagttc
    31381 aaaactcatt cacggtggcc tgcgctacct tgagcactat gaattccgcc tggtcagcga
    31441 ggcgctggct gaacgtgaag tgctgctgaa aatggctccg catatcgcct tcccaatgcg
    31501 ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg atgattcgga ttggtctgtt
    31561 tatgtacgat catctgggta aacgcaccag cctgccggga tcaaccggtt tgcgttttgg
    31621 cgcaaactca gtgttaaaac cggaaattaa gcgcggattc gaatattctg actgttgggt
    31681 agacgacacc cgtctggtac tcgccaacgc ccagatggtg gtgcgtaaag gcggcgaagt
    31741 gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac ggcctgtgga ttgtggaagc
    31801 ggaagatatc gataccggca aaaaatatag ctggcaagcg cgcggcctgg ttaacgccac
    31861 cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat ctgccttcgc cttatggcat
    31921 tcgcctgatc aaaggcagcc atattgtggt gccgcgtgtg catacccaga agcaggccta
    31981 cattctgcaa aacgaagata aacgtattgt gttcgtgatc ccgtggatgg acgaattttc
    32041 catcatcggc accaccgacg tagagtataa aggcgatccg aaagcggtga agattgaaga
    32101 gagtgaaatc aactatctgc tgaaagtgta taacgcgcac tttaaaaaac agcttagccg
    32161 tgacgatatc gtctggacct actccggtgt gcgtccgctg tgtgatgatg agtccgactc
    32221 gccgcaggct attacccgtg attacaccct tgatatccat gatgaaaacg gcaaagcacc
    32281 gctgctgtcg gtattcggcg gtaagctgac cacctaccga aaactggcag aacatgcgct
    32341 ggaaaaactg acgccgtatt atcagggtat tggcccggcg tggacgaaag agagcgtgct
    32401 gccgggtggt gccattgaag gcgaccgcga cgattacgca gctcgtctgc gccgtcgcta
    32461 tccattcctg actgaatcgc tggcgcgtca ttacgcacgc acttacggca gcaacagcga
    32521 gctgctgctc ggcaatgcgg gggcgataag cgacctcggg gaggatttcg gtcatgagtt
    32581 ctacgaagcg gaactgaaat acctggtgga tcacgaatgg gtccgccgca ccgacgatgc
    32641 cctttggcgt cgcacaaaac aaggcatgtg gctgaatgcg gatcaacaat ctcgtgtgag
    32701 tcagtggctg gtggaatata cgcagcagaa gttgtcgctg gcgtcgtaag gatgatgccc
    32761 ggttgaagtg caaccgggct atttggattc cttagactat tcattgtaaa taattgaata
    32821 acggcccctg agctaaaatc gccaaactta agcacgttac ccaatcgccc cacttcttcc
    32881 tctggtttct attttttctg caccaatggc aataatatcc caatgcaaaa ccaagctgtg
    32941 cactaatcgg gccgacaaaa tcagtccaag gctgcggcca aaaaggggaa aaattcaggg
    33001 gaggccagac aaacgtcaga aagaaatttc ggatattttc cagcaacgct gatttctctg
    33061 gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccggc gaaaagacca
    33121 gccagcaatg cagtctgttt tttaattcag gaggcgttct gcgggagaat tgccaaccga
    33181 tgccagccat tacactgaaa aaaagtgcat attcaaccat taactcaagt tgtaattcct
    33241 gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttctgcc agaaaagtta
    33301 acgtgaatat aagtacggct aagacaataa ccgtcggcca taccaataat ggcacgataa
    33361 caaaaccgag aattacaggg ggaatataaa gccggaatct aatcattttt attttcctgg
    33421 tgattcggat gatgcgtcat acatcgcgca taaacactca tcaattatcc agtcatcttc
    33481 ccagacctga tattctgtca ttggttcggg ctgttttagc gggttctcaa tcgcttgccg
    33541 cgctctttct ttcagtaaag gatgattatt tctagcccag gcattgattt gggtggtaat
    33601 gacgttatcg atatcgtcct ggttatctct gatataatcc tcacaatatc tgttcaataa
    33661 accggcaaga gaaacggtcg accaaaagcc gggaactgaa ttaatggcat ccagcaatgg
    33721 cgcaagagcg atatcggcac ggaaaaacat ttccagcata tactgtaatt cgacagcttc
    33781 atcgccggat acccaatcag tcatatactg gcaggcaaat cgctgtaaaa ttgcccgttc
    33841 atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa aaactacgtg
    33901 aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa aatagcggac
    33961 ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga ttaaggaata
    34021 cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac tcaggcaata
    34081 ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt gataaatctg
    34141 ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc ccgctgatcg
    34201 cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat aagggcgcta
    34261 cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct ttcctctgcg
    34321 caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt atcgcgaaat
    34381 ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taacgacctg aaagatatag
    34441 acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat cacccgctca
    34501 tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt caggtcacat
    34561 tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc acaggcacaa
    34621 agacggctaa tggtggcggt atcgagggaa aatcccggcc atactttgct ggaggttaag
    34681 ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc accgacgaac
    34741 ttttcccctt catccagggc ctggttaata agttgctgaa caatgccttc atcccagtat
    34801 gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt accggcaagg
    34861 cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg tagcgtctta
    34921 tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt taaaaaccac
    34981 tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa ggcatcgatg
    35041 atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc gttaatcgct
    35101 agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa ggtgtaacct
    35161 attgcgttgt ttgtattacg gtattcggca acgactttaa tcattccctc catcatactt
    35221 gccacttctg tttcctgcgg cgagatcatg cggacatttt tcatgacctg ggattgcatc
    35281 accgtctggc taccagagtc ttccgggcgt tgctaggtct ggatctcctg atcgttaccg
    35341 ccaacagtac gccagttggt aattgcacca ctgaagatgt cacgcacctg ttgttcagtc
    35401 agggaattaa ccggattatc cgcattgacg atgaaaacaa acgcttcacg ggcaaaaggc
    35461 gtgtacagca aagtaatgcc cgattcctca gcgcgttttt tctgcccacc ggaaggttgc
    35521 gccacaaaaa taatatcagc atcaccttta acaatcctgt tatacgcatc gggggtacga
    35581 gagctctcca gatattccct ggtgtgaaaa tcttccggta taacgattaa agcataaaat
    35641 gcagaggcat aaattgggta cgccgccgta gcaccatcga gtcgcggcca gttttgcgta
    35701 aactgaattt gcggtttacc acgcagtggt gtcagttgat tattgagttt atcgggtcgc
    35761 caggcccagg tatcaatatt ttcgcgcaca ccgacgccgg gattgagctg tttatataaa
    35821 taaccctgat aaccagcgac aaaaccaagc atgagaagga taaacaggag ggaattacgg
    35881 caacgtagcc cggatgtacc ggttaaagga tgtctggttg gccggccgta ccccagcgaa
    35941 aaactgatat gcgcgccaac gggtataatg actatccacc agtactcacc tccgactact
    36001 gagactaaca aaacgcccag ccaggggagc ataagatcag ccaaccaacc cgatcccatc
    36061 tggtgaaatt gataatctgc cagatgcagc gcgattatcc agccaaccag gattaataaa
    36121 ccgggtacta aaaaaagaca ttgtcgccag acggctattt ctcttttttc tggtgcacta
    36181 tcgcctgccc atactcgccc cgagaaaaat agcattaatg cgcccagcgc cagcataaaa
    36241 atgttgagca gaaggtaact tgttggcgtg ataaggatat tacaaacttc aataataccg
    36301 gcaagtccga cacccagcat agcaacaacc gccgccaaaa attgcgtcag tatggggatg
    36361 ccgaaaaaag tcattaccag cgaggacaaa atccattttc tgttttgcat tattctttcc
    36421 attctttttg aatggtgaaa ttatactccc cgagtcccct tgccccttct ggacactttt
    36481 ccgaaatgat ggcggaaaaa aacgggaccc tttg
//
LOCUS       DAJTMK010000020        35262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTMK010000020 DAJTMK010000000
VERSION     DAJTMK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35262
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.020"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..182
                     /locus_tag="OO135_000844"
     CDS             <1..182
                     /locus_tag="OO135_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5396785.1"
                     /translation="TRSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
     gene            208..921
                     /gene="msyB"
                     /locus_tag="OO135_000845"
     CDS             208..921
                     /gene="msyB"
                     /locus_tag="OO135_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5396786.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIPCLRRMVSATQV"
     gene            1070..1636
                     /gene="satP"
                     /locus_tag="OO135_000846"
     CDS             1070..1636
                     /gene="satP"
                     /locus_tag="OO135_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5396787.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1671..2258)
                     /gene="mog"
                     /locus_tag="OO135_000847"
     CDS             complement(1671..2258)
                     /gene="mog"
                     /locus_tag="OO135_000847"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5396788.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2373..3326)
                     /gene="tal"
                     /locus_tag="OO135_000848"
     CDS             complement(2373..3326)
                     /gene="tal"
                     /locus_tag="OO135_000848"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5396789.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3605..5029
                     /locus_tag="OO135_000849"
     CDS             3605..5029
                     /locus_tag="OO135_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR5396790.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLI
                     QKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRICTF
                     ATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPV
                     FDPLRYPDIGRQLSPDAWDDVSQE"
     gene            5099..5875
                     /gene="yaaA"
                     /locus_tag="OO135_000850"
     CDS             5099..5875
                     /gene="yaaA"
                     /locus_tag="OO135_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5396791.1"
                     /translation="MLILISPAKTLDYQGPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6028..6324)
                     /locus_tag="OO135_000851"
     CDS             complement(6028..6324)
                     /locus_tag="OO135_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5396792.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGLGKHHR"
     gene            complement(6538..7824)
                     /gene="thrC"
                     /locus_tag="OO135_000852"
     CDS             complement(6538..7824)
                     /gene="thrC"
                     /locus_tag="OO135_000852"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5396793.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(7825..8757)
                     /gene="thrB"
                     /locus_tag="OO135_000853"
     CDS             complement(7825..8757)
                     /gene="thrB"
                     /locus_tag="OO135_000853"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5396794.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(8759..11221)
                     /gene="thrA"
                     /locus_tag="OO135_000854"
     CDS             complement(8759..11221)
                     /gene="thrA"
                     /locus_tag="OO135_000854"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5396795.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11247..11366)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11302..11367)
                     /gene="thrL"
                     /locus_tag="OO135_000855"
     CDS             complement(11302..11367)
                     /gene="thrL"
                     /locus_tag="OO135_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5396796.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(11581..12267)
                     /locus_tag="OO135_000856"
     CDS             complement(11581..12267)
                     /locus_tag="OO135_000856"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5396797.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(12667..12807)
                     /locus_tag="OO135_000857"
     CDS             complement(12667..12807)
                     /locus_tag="OO135_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396798.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            12903..13619
                     /gene="arcA"
                     /locus_tag="OO135_000858"
     CDS             12903..13619
                     /gene="arcA"
                     /locus_tag="OO135_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5396799.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(13679..14977)
                     /gene="creD"
                     /locus_tag="OO135_000859"
     CDS             complement(13679..14977)
                     /gene="creD"
                     /locus_tag="OO135_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5396800.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLGTSVL
                     VVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK"
     gene            complement(15035..16459)
                     /gene="creC"
                     /locus_tag="OO135_000860"
     CDS             complement(15035..16459)
                     /gene="creC"
                     /locus_tag="OO135_000860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5396801.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLYDSANKAVGQDYSRWNDVWLTLHGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVVAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(16459..17148)
                     /gene="creB"
                     /locus_tag="OO135_000861"
     CDS             complement(16459..17148)
                     /gene="creB"
                     /locus_tag="OO135_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5396802.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17161..17634)
                     /gene="creA"
                     /locus_tag="OO135_000862"
     CDS             complement(17161..17634)
                     /gene="creA"
                     /locus_tag="OO135_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5396803.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            17845..18714
                     /gene="robA"
                     /locus_tag="OO135_000863"
     CDS             17845..18714
                     /gene="robA"
                     /locus_tag="OO135_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5396804.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(18711..19358)
                     /gene="gpmB"
                     /locus_tag="OO135_000864"
     CDS             complement(18711..19358)
                     /gene="gpmB"
                     /locus_tag="OO135_000864"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5396805.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTQRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSGRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19410..19931
                     /gene="yjjX"
                     /locus_tag="OO135_000865"
     CDS             19410..19931
                     /gene="yjjX"
                     /locus_tag="OO135_000865"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5396806.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(20016..20342)
                     /gene="trpR"
                     /locus_tag="OO135_000866"
     CDS             complement(20016..20342)
                     /gene="trpR"
                     /locus_tag="OO135_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5396807.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(20432..22369)
                     /gene="sltY"
                     /locus_tag="OO135_000867"
     CDS             complement(20432..22369)
                     /gene="sltY"
                     /locus_tag="OO135_000867"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5396808.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATSAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22580..24247
                     /gene="ettA"
                     /locus_tag="OO135_000868"
     CDS             22580..24247
                     /gene="ettA"
                     /locus_tag="OO135_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5396809.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(24554..25786)
                     /gene="nadR"
                     /locus_tag="OO135_000869"
     CDS             complement(24554..25786)
                     /gene="nadR"
                     /locus_tag="OO135_000869"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5396810.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(25807..27189)
                     /gene="radA"
                     /locus_tag="OO135_000870"
     CDS             complement(25807..27189)
                     /gene="radA"
                     /locus_tag="OO135_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5396811.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(27238..28206)
                     /gene="serB"
                     /locus_tag="OO135_000871"
     CDS             complement(27238..28206)
                     /gene="serB"
                     /locus_tag="OO135_000871"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5396812.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            28312..28956
                     /locus_tag="OO135_000872"
     CDS             28312..28956
                     /locus_tag="OO135_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5396813.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGTSWFSQNHQRQRNPQ
                     LEELTRPLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTLWQQSPFLLTASKPVPEEEESEKKE"
     gene            28984..30000
                     /gene="lplA"
                     /locus_tag="OO135_000873"
     CDS             28984..30000
                     /gene="lplA"
                     /locus_tag="OO135_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR5396814.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            30032..30181
                     /locus_tag="OO135_000874"
     CDS             30032..30181
                     /locus_tag="OO135_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396815.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(30456..31175)
                     /gene="deoD"
                     /locus_tag="OO135_000875"
     CDS             complement(30456..31175)
                     /gene="deoD"
                     /locus_tag="OO135_000875"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5396816.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(31255..32478)
                     /gene="deoB"
                     /locus_tag="OO135_000876"
     CDS             complement(31255..32478)
                     /gene="deoB"
                     /locus_tag="OO135_000876"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5396817.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(32530..33852)
                     /gene="deoA"
                     /locus_tag="OO135_000877"
     CDS             complement(32530..33852)
                     /gene="deoA"
                     /locus_tag="OO135_000877"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5396818.1"
                     /translation="MFLAQEIIRKKRDGHALNDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(33979..34758)
                     /gene="deoC"
                     /locus_tag="OO135_000878"
     CDS             complement(33979..34758)
                     /gene="deoC"
                     /locus_tag="OO135_000878"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5396819.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            35016..>35262
                     /locus_tag="OO135_000879"
     CDS             35016..>35262
                     /locus_tag="OO135_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCR5396820.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
BASE COUNT         8356 a         9598 c         8827 g         8481 t
ORIGIN      
        1 ccacccgtag cgccagtcag agtctgaata ttccttcaga aataaaagaa gggcaaacca
       61 ctgactggat caacattaac agtgataacg acaataaacg ctgcgtctca aaaatcacct
      121 tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc ggcgacgact
      181 aacgccagaa tatagaagcc acaaaaaatg aatgttaatt acctgaatga ttcagatctg
      241 gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt gctcacccat
      301 aatgaaaaag gcaaaactcg cctctccagc gtactgatgc gcaacgaact gtttaaatcg
      361 atggaagggc atcccgagca acatcgccgc aactggcagc tgattgctgg agaattacag
      421 cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt gtatcgggcc
      481 attttgctcg atgtttcaaa acgattgaag ctgaaagccg acaaagagat gtctacgttt
      541 gaaattgagc aacagttact ggaacaattt ctgcgtaata cctggaagaa aatggacgag
      601 gaacataagc aggagtttct gcacgcgttc gatgcgaggg tgaatgagct ggaagagctg
      661 ctgccgctgc tgatgaaaga caaattattg gcaaaaggcg tgtcgcattt gctttccagc
      721 caactgaccc gcattttacg cacccacgca gcaatgagcg tacttgggca tggtttgctg
      781 cgcggcgcgg ggctgggagg ccctgtaggt gcggcactaa atggggttaa agcggtcagc
      841 ggcagcgcct atcgcgtgac gattccagcc gtactgcaaa tcccctgcct gcgccggatg
      901 gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa ttttcatttt cccctcaaaa
      961 gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac actacagtta
     1021 ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta tgggcaacac
     1081 taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca ccattctgct
     1141 taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca tgggcatttt
     1201 ctacagcggc atcgcgcaaa tttttgctgg tctgctggag tacaaaaaag gcaacacttt
     1261 cggtttaacc gcattcacct cttacggttc tttctggctg acgctggttg cgattctgct
     1321 gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg tctacctggg
     1381 tctgtggggc gtatttacgc tgtttatgtt cttcggcacg ctgaaaggcg cacgcgttct
     1441 gcaattcgtt ttctttagcc tgactgtgct gtttgccctg ctggcgatcg gtaacattgc
     1501 cggtaacgcc gcaatcatcc actttgccgg ctggattggt ctgatctgtg gtgccagtgc
     1561 aatctatctg gcgatgggtg aagtactgaa cgagcagttt ggtcgcaccg ttctgccgat
     1621 tggtgaatcc cactaattgt tttgagatcc ccccgctcgg ggggattttt ttattcgcta
     1681 acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc cgtttcaacg
     1741 tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc gtgtaccaca
     1801 acgttacccg cagcgtcctt cacaccttcc agcgtctctt taatagactt cggctgaccg
     1861 ggtaagttaa ggatcagcgc ctgtttgcga atcacaccaa cctgacgcga aaggatcgca
     1921 gttggtacaa aatgcaggct tatctggcgc atctgttcac caaagccagg catctcgcgg
     1981 tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc agttccgccc
     2041 gtggtgagca ccagatggca actcatttca tccactagct cacacaacgt ttgctcgatg
     2101 atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt tagcgccgat
     2161 gtcagccatt cttccagcgc agggatgcct ttatcctgat aaacgccgct ggatgcgcga
     2221 tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc gtttaaacag
     2281 tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca gaagaggtag
     2341 cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat cattttttcc
     2401 agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc tactgccatt
     2461 ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg acgcgctttc
     2521 acttcgccgg tgtaagacag tttacgttcg atagcccctt cgctctcagc cagctctttc
     2581 agcagtgtag gtgcgatggt aagacggtcg cagcctgcca gttccagaat ttcgccgatg
     2641 ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta gtactggtag
     2701 atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt cttatcagta
     2761 ttcgctttgt accagtcaag aatacggcca acaaatggcg agatcaggaa cacgcctgct
     2821 tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca gttgatgcct
     2881 tctttttcca gctgttctgc agcacggata ccctgccagg tagaagccag tttgatcaga
     2941 atacgatcgt tgctaatacc agcatcgttg tagagtttga tcaggcgttt tgctttcgca
     3001 attgacgctt cggtgtcata ggaaagacgc gcatcaactt cagttgagat acgacccgga
     3061 accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc gtccacgatc
     3121 tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc aatcaactta
     3181 cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt ggcatcctgc
     3241 ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt ggtgtactga
     3301 cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct tgttaggggg
     3361 atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg ccgacaagac
     3421 ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc gcccgccatt
     3481 tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta gcgtatatac
     3541 ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa taagagggat
     3601 atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg taatgattta
     3661 cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc agtttcgcta
     3721 catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag gcggtttaac
     3781 ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca acctggccgg
     3841 cgttgcgctg gctattaccg ccggtggacc tagagccgtc ttttggatgt gggttgccgc
     3901 gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt ataaagaacg
     3961 tgacgtcaat gggcagtttc gtggcggacc ggcatggtat atggcgcgcg ggctggggat
     4021 gcgttggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca taattttcag
     4081 cggagttcag gcgaacgccg ttgcccgagc cctgagtttt tcttttgatt ttcccccgct
     4141 ggtgacaggc attattctcg ctgtctttgc tctgctggca atcactcgcg gtcttcatgg
     4201 cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg ataatctggg tgctgaccag
     4261 catggtgatt tgcgtaataa atatcgggca acttccccac gtcatttggt ctatttttga
     4321 gagtgctttt ggctggcagg aagcggcagg cggcgcggcg ggatatacct taagccaggc
     4381 gattactaac ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg gttcgacgcc
     4441 aaacgcggca gcggcgtcct ggcctccgca tccggcagcg caagggattg tccagatgat
     4501 tggcattttt atcgacaccc tggtcatctg tacggcaagc gccatgctga tattactggc
     4561 gggtaacgac acaacctaca tgccgctgga aggtattcag cttatccaga aggcgatgcg
     4621 ggtgctaatg ggttcctggg gtgctgagtt tgttaccctc gtggttattc tgtttgcctt
     4681 cagctccatc gttgccaact acatttatgc cgaaaacaat ctcttctttt tacgcctgaa
     4741 cacccctaaa gcgatctggt gtttgcggat ctgcaccttc gcaacggtca tcggcggcac
     4801 cttgctaagc cttccgctga tgtggcaact ggcagatatc ataatggcct gcatggctat
     4861 taccaatttg accgccattt tactgctctc gcctgtggtt cataccattg ccagtgatta
     4921 tctacgccag cgtaaactcg gcgtgcgccc ggtgtttgat ccgttgcgtt atccggatat
     4981 cggtcgccag ctttctccgg acgcgtggga tgatgtttcg caggagtaat cacaactatc
     5041 gatcaactca ttctcttttt ttgctaaagt cggcataaat ttcctgcaag gactggatat
     5101 gctgattctt atttcacctg cgaaaacgct tgattaccaa ggcccgttga ccaccacgcg
     5161 ctatacgctg ccggagctgt tagacaattc ccagcagttg atccatgaag cgcggaaact
     5221 gacgcctccg cagattagca cgctgatgcg catcagcgac aaactggcgg gtatcaacgc
     5281 cgctcgcttt catgactggc agccagattt cacgccggcg aatgcccgcc aggcgattct
     5341 ggcgtttaaa ggtgatgtct acaccggctt gcaggctgaa accttcagcg aagacgattt
     5401 cgattttgcc caacagcatt tgcgaatgct ttccggcttg tatggcgtac tgcgcccgct
     5461 cgatttaatg cagccttatc gtctggaaat ggggatccgt cttgagaatg cccgagggaa
     5521 agatctgtat caattctggg gagatatcat caccaacaag ctgaacgagg cgctcgcagc
     5581 acaaggcgat aatgtggtga ttaatctggc gtcagatgag tattttaaat cggtgaagcc
     5641 gaagaaattg aatgccgaga ttatcaagcc ggtgttcctc gatgagaaga acggcaaatt
     5701 taagatcatc agcttctacg ctaagaaagc acgcggtctg atgagtcgtt tcattattga
     5761 aaatcggctg accaaaccag agcaactgac tggttttaat agcgaaggtt acttctttga
     5821 tgaagcttcc tccagcaatg gcgaactggt gtttaaacgc tacgagcagc gttaagtttg
     5881 cagtcaatgc cggatgcggc gtgaacgcct tatccggcct acaaaagcat gcagattcaa
     5941 tatattgcag aattcacgta ggcctgataa gacgcgccag cgtcgcatca ggcgctgaat
     6001 gccggattgt tacccggcat ttgtcattta gcggtgatgt ttgcctagac catgaccgcc
     6061 gtgatgatca tgaggagctt tcttatggtg gcgcggcggt ggcggcggtc cgtgtgggtg
     6121 ccagcgattg cctcgccatt cataatgttg tttccaccag ccgtggtcgc gccagtgacc
     6181 tccatcccag taatagccac gattatcacg atcgcctatc tgtaatttta ctgacgggac
     6241 taacgtaatt tccgcagcct gtgctgccat gggagcgacc agaaccaggg aaagtgcgag
     6301 tacgatagat tgcatctttt tcacaggtta ctccttttat cattgcccgt tatgggtcga
     6361 tatccggaga ttacgcgatg agaacgacgg ttgttattct ccgcaatcct aatctctaag
     6421 ttaccgcatt tattgagaat ttctcctttt tccctgtcgt ttttctccat aatttcttca
     6481 taaaaaagcc gggatgcata aaagcaaacc cggcctgatt gagataatga atagatttta
     6541 ctgatgattc atcatcaatt tacgcaacgc agcaaaatcg gcgggcagat tatgtgaaag
     6601 caagggtaaa tcagcacgtt ctgccagctc ttttggcaga tccaacgttt caccgagaat
     6661 cgcttccacg ctctctttaa atttcgccgg atgcgcggtg ccgaggaaca agccatattc
     6721 gcctggattc aactggtcac gcagcgcacg atacgctacg gcagcgtgcg gctccgaggt
     6781 gtagcccagt tcttttaact cacgcattgt ctgttgcgtg gtttcatcat ccacggctgc
     6841 ataacccagc tctttcagtt gccagatttt gcggcggaac aactcttcca cacgcggcca
     6901 gttgttcggc tgactcacgt ccatcgcgtt ggataacgtc gcctgagtcg ctttgggtga
     6961 ccactgaccg tcgtgcagga aacgtggcac ggtatcgttc acgttggtcg cagcaataaa
     7021 acgtttcacc ggcagaccga gtgacttcgc cagcagaccc gccgtcaaat cgccgaagtt
     7081 tccgcttggc accgagacaa ccagctggtt gcgcgcttcc tgcggcagct gcgcaactgc
     7141 ttcaaagtag tagcaaatct gcgccagcaa acggctgatg ttaatcgagt tagctgagtt
     7201 taaccccagc gccactttca gctcttcatc atcaaacgcc tgcttcacca gcgcctgaca
     7261 ggcatcgaaa tcgccgtcga tggcaacagt ttcgatattg ccgcccaatg tacagaacag
     7321 tttttcttgc agtggactga ttttgcctcg tggatagagg ataaccactt tcacattcgg
     7381 taaaccgtag aaagcatgag ccactgccgc tccggtatca ccggaggtcg cggtcagaat
     7441 ggtcactggc ttatcgcccg caatatgggt cagcatttgt gccataaagc gaccgccgaa
     7501 atctttaaat gccagcgttg gcccgtggaa caattccaga caaccgacat cgctttcaac
     7561 attggcgacc ggagccggga aggcaaacgc cgcgcgcacg cgctcttcca ggatttcctg
     7621 cgggatttca tcaccaataa acgccgagag gatcttcgca ctgcgggtga caaaatccag
     7681 cttcagcatc tcatcaattt cagtcaggct gaattccggc aggtcgtgcg gaaaaaacag
     7741 cccctgattt ttgcccaacc cctgggttac ggcttgcgca aagctgacct gctcattgtg
     7801 atctttcaga ttgtagagtt tcatttagtt ttccagtact cgtgcgcccg ccgtatccag
     7861 ccggcaaata tgaacaaaac cttcctgatt ttgcaggtag ttcttaccca accagtcggc
     7921 aacgcgctgg gcggtatccg gcttgtcaca cagagcgaac aaggtcgggc cggagccgga
     7981 gataccgctc gctaccgcgc cgatttccgc aaccgcctgc cgcgcctgcc ggaagccagg
     8041 cagtaaccgt tcacggtagg gttcagcgat aacatctttc atcagcttcg cggcaagctc
     8101 aggctgacgg gaatagcagg cgtgaatgaa gccagccaga tgtcgcccgt gcgcaatgca
     8161 atcctggcgg cgatactgcg ccggtaaaat agccctggct tctgccgttg agactttaat
     8221 ccccggatac gccagcaccc acagccactc atcaaaccct ggcacttgct ggctgatgat
     8281 gtcgttttct tcgatcatca actgcatacc accaagaaaa cacggtgcca cgttgtcgta
     8341 atgaatgctg ccggagattc gtccttccag ctcgcccatc aaagccagca aacgagtgtc
     8401 attaagcggc ttgccacagt gttcattcat cgccatcagc gccgcgacca ccgaacaggc
     8461 gctggagcct aagcccgaac cgattggcat attcttttcc agagtcatcg ccactggaat
     8521 ttgcttgcca agctcctggc aaaaacgctc ccagcactga taaacgatat tttcccgtgg
     8581 ttctgacggc agcttatcgg caaagcgtcc gaggttgttg agactgaatg tctctgccgc
     8641 ctcaaccgtg actacatctc cgagcaatgc accatcaaca ggtgtcaccg ccgccccgag
     8701 cacatcaaac ccgacgctca tattggcact ggaagccggg gcataaactt taaccatgtc
     8761 agactcctaa cttccatgag agggtacgta gcagatcggc aaagacaccg gcagctgtaa
     8821 cgtcattgcc cgcaccatat ccgcgcagca ccaacggcag cggctgataa tagtggctat
     8881 aaaaggccag ggcgttttcg ccatttttca ctttgaacag cggatcatta ccatccactt
     8941 cggcaatctt cacgcggcag gcaccatctt catcaatatt gccaacatag cgcaaaactt
     9001 ttccttcatc acgggccttc gccacgcgcg cggcaaagag atcgtcgagc tgtgacagat
     9061 tcgccataaa agcggcaaca tcaccctcag cgttaaactc tgcgggcagc acaggttcaa
     9121 tttcaatatc cgccagctcc agttcacgtc ccgtttcacg agcgagaatc aatagcttac
     9181 gcgctacatc cataccagaa agatcatctc gcggatccgg ttcggtataa cccatttccc
     9241 gcgccagagt agtcgcctcg gagaaactca tgccttcgtc taacttgccg aagatataag
     9301 aaagcgaacc tgaaagaatg ccggagaact tcatcaattc atcaccagca ttgagcagat
     9361 tttgcaggtt ctcaataacc ggtaatccag ccccaacgtt ggtgtcatag aggaatttac
     9421 gccgcgattt ttccgccgca tgacgcaaca gatggtagta atccatcgac gaggtgttgg
     9481 cctttttgtt cggcgtgaca acgtggaaac cttcgcgcaa gaagtcggca tattgatccg
     9541 ccactgcctg gctggaggtg cagtcaacaa tgaccgggtt cagcagatga tattctttca
     9601 cgaggcgaat taagcgcccg agattaaacg gctctttggc ttgcgccagt tcttcctgcc
     9661 agttttccag atttaggcca tgcacattgg tgagcagtgc cttcgagttg gcaacaccgc
     9721 agacacgtaa gtcgatatgt ttattcttca gccagctttg ttgacgcttc agttgctcca
     9781 gcagcgcacc gccaacgcca ccgacgccaa tcacaaacac ttcgataacc tgatcggtat
     9841 tgaacagcat ctgatgagta acgcgcacgc cagtggtcgc atcatcgtta tttaccacga
     9901 cagagattga gcgttcagaa gatccctgag caatggcgac aatgttgata ttggcgcggg
     9961 ccagcgcggc aaagaatttc gccgagatcc cacgcaaggt gcgcatacca tcacctacca
    10021 ccgagataat ggccagccgt tccgtcaccg ccagcggctc cagtaagcct tctttcagtt
    10081 ccaggtagaa ctcttcctgc attgcccgtt cagctcgcac acagtcgctt tgtggaacgc
    10141 agaaactgat actgtattcg gaagatgatt gcgtaatcag caccacggaa atacgggcgc
    10201 gtgacattgc agcaaagacg cgcgccgcca tgccgaccat tcctttcatc cccggaccag
    10261 aaacgctgaa cattgccata ttattcagat tggaaatgcc tttgaccggt aattcgtctt
    10321 catcacggct ggcaccaatg agcgtacctg gtgcttgagg atttccggta tttttaatca
    10381 ggcaagggat ctggaactgg gcgatggggg taatggtgcg ggggtgaaga actttagcgc
    10441 cgaagtagga aagctccatc gcttcctggt aggacatcga cttcaacaac ctcgcatcgg
    10501 gcacctgacg cgggtcgcag gtatagaccc cgtcaacgtc cgtccaaatc tcgcaacaat
    10561 cggcgcgtaa acaggcagcc agcaccgccg cggagtagtc ggaaccgtta cgtccaagta
    10621 ccaccagttc gcctttttca ttaccggagg tgaaacctgc catcaacacc atgtgatcag
    10681 ccggaatgcg gcttgccgca atacggcggg tggactcagc aatatcgacg gtagattcga
    10741 ggtaatgccc cactgccagc agtttttcga ccggatcgat aacggtaacg ttgtgaccac
    10801 gcgcttctaa cacgccggcc ataatggcga tcgacatttt ctcgccacgg caaatcagcg
    10861 cagcgttgat gctatccggg cactgcccca acaaactaat gccatgcagg acatgtttta
    10921 tttgggcaaa ttcctgatcg acgaaagttt tcaattgcgc cagcgggaac cccggctggg
    10981 cggcggcgag tcccgtcaaa agttcggcaa aaatacgttc ggcatcgctg atattgggta
    11041 aagcatcctg gccgctaatg gttttttcaa tcatcgccac caggtggttg gtgattttgg
    11101 cgggggcaga gaggacggtg gccacctgcc cctgcctggc attgctttcc agaatatcgg
    11161 caacacgcag aaaacgttct gcatttgcca ctgatgtacc gccgaacttc aacactcgca
    11221 tggttgttac ctcgttacct ttggtcgaaa aaaaagcccg cactgtcagg tgcgggcttt
    11281 tttctgtgtt tcctgtacgc gtcagcccgc accgttacct gtggtaatgg tgatggtggt
    11341 ggtaatggtg gtgctaatgc gtttcatgga tgttgtgtac tctgtaattt ttatctgtct
    11401 gtgcgctatg cctatattgg ttaaagtatt tagtgaccta agtcaataaa attttaattt
    11461 actcacggca ggtaaccagt tcagaaactg ctatcagaca ctcttttttt aatccacaca
    11521 gagacatatt gcccgttgca gtcagaatga aaagctgaaa aatacttact aaggcgtttt
    11581 ttatttggtg atattttttt caatatcatg cagcaaacgg tgcaacattg ccgtgtctcg
    11641 ttgctctaaa agccccaggc gttgttgtaa ccagtcgacc agttttatgt catctgccac
    11701 tgccagagtc gtcagcaatg ccatgactcg ttcgcgtaaa gcttgcagtt gatgttggtc
    11761 tgccgttgtg tcacttttcg tcggttgttg tattaatgtt gctaattgat agcaatagac
    11821 catcaccgcc tgccccagat tgagcgaagg ataatccgcc accatcggca caccagtaag
    11881 aacgtcagcc aacgctaact cttcgttagt cagcccggaa tcttcgcgac caaacaccag
    11941 cgcggcatgg ctcatccatg aagatttttc ctctaacagc agcaccagtt ctactggcgt
    12001 ggcgtagtaa tgatatttcg cccgactgcg cgcagtggtg gcgacagtga aatcgacatc
    12061 gtgtaacgat tcaaccagtg tcgggaaaac tttaatatta tcaataatat caccagatcc
    12121 atgtgcgacc cagcgagtgg ctggctccag atgtgcctga ctatcgacaa tccgcagttc
    12181 gctaaaaccc atcgttttca ttgcccgcgc cgctgcccca atattttctg ctctggcggg
    12241 tgcgaccaga ataatcgtta tacgcatatt gccactcttc ttgaccaaat gaccgcgaac
    12301 agggtgatca ctgtcaactt attacgcggt gcgaatttac aaatccttaa cgtaagtcgc
    12361 agaaaaagcc ctttacttat cttaaaaaag gctaaactat ttcctgactg tactaacggt
    12421 tgaattgtta aaaaatgcta catatccttc tgtttactta ggataatttt ataaaaaata
    12481 aatctcgaca attggattca ccacgtttat tagttgtatg atgcaactag ttggattatt
    12541 aaaataatgt gacgaaagct agcatttaga tacgatgatt tcatcaaact gttaacgtgc
    12601 tacaattgaa cttgatatat gtcaacgaag cgtagtttta ttgggtgtcc ggcccctctt
    12661 agcctgttat gttgctgtta aaatggttag gatgacagcc gtttttgaca ctgtcgggtc
    12721 ctgagggaaa gtacccacga ccaagctaat gatgttgttg acgttgatgg aaagtgcatc
    12781 aagaacgcaa ttacgtactt tagtcatgtt acgccgatca tgttaatttg cagcatgcat
    12841 caggcaggtc agggactttt gtacttcctg tttcgattta gttggcaatt taggtagcaa
    12901 acatgcagac cccgcacatt cttatcgttg aagacgagtt ggtaacacgc aacacgttga
    12961 aaagtatttt cgaagcggaa ggctatgatg ttttcgaagc gacagatggc gcggaaatgc
    13021 atcagatcct ctctgaatat gacatcaacc tggtgatcat ggatatcaat ctgccgggta
    13081 agaacggtct tctgttagcg cgtgaactgc gcgagcaggc gaatgttgcg ttgatgttcc
    13141 tgactggccg tgacaacgaa gtcgataaaa ttctcggcct cgaaatcggt gcagatgact
    13201 acatcaccaa accgttcaac ccgcgtgaac tgacgattcg tgcacgcaac ctgctgtccc
    13261 gtaccatgaa tctgggtact gtcagcgaag aacgtcgtag cgttgaaagc tacaagttca
    13321 atggttggga actggacatc aacagccgtt cgttgatcgg ccctgatggc gagcagtaca
    13381 agctgccgcg cagcgagttc cgcgccatgc ttcacttctg tgaaaaccca ggcaaaattc
    13441 agtctcgtgc tgaactgctg aagaaaatga ccggccgtga gctgaaacca cacgaccgta
    13501 ctgtagacgt gacgatccgc cgtattcgta aacatttcga atctacgccg gatacgccgg
    13561 aaatcatcgc caccatccac ggtgaaggtt atcgcttctg tggtgatctg gaagattaat
    13621 cggctttacc accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt
    13681 atttccagat acgtaattga tcgtccattg taacttcttt actggctttc attttcggca
    13741 gtgaaaacgc ataccagtcg atattacgag tcacaaacat catgccggcc agcgccacca
    13801 ccagcacact ggttcccaac aacagcgcga gggtaaacaa catgctgttg cgccagcctt
    13861 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
    13921 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacatcacca
    13981 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
    14041 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
    14101 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt
    14161 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
    14221 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggt agaaaatcac
    14281 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
    14341 gcaccacaga aagatcgccg gtaccgctta aattcagtgc catattcagc tgcaggttct
    14401 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
    14461 cggtaccggg ttcaatggtc agtgccgttc cgttaacttc aggcgctttc accacaccaa
    14521 taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg atatttggcg
    14581 cgttgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
    14641 agacctgacc ggtataaatc cctatcttgc gttcttccac gttttgattg ccatcaacca
    14701 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
    14761 cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca acgagttttt
    14821 gcggcccgct ggtactttgg cgaatcgcgt cttccacatc gctacggtaa tcggcacgtt
    14881 cgacaatcac ttgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
    14941 aggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt gcaatggcag
    15001 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
    15061 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
    15121 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
    15181 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
    15241 tatccagcac cttaagggcg acgtgttcct gatccacttc ggcgcttagc gttatgcgac
    15301 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgctccagta
    15361 acgccggttc agcaacaaca ttaacctcag tgggcataac atgtagagtg atgttttttt
    15421 ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa taatgccgcc acatcaacaa
    15481 cagtcagaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttctacca
    15541 atgcctgcat acgcgcattt tgcgtcagaa tgttgtcggt aaaacgagcc accacttccg
    15601 gtggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
    15661 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
    15721 gcatactttc cagcgcctgc gcaagcttac gcaactcgct actgccgagt tcaggaagag
    15781 gaacgggctt attgtcagtg acggaatcgg catagcgagt gagccgggca atggagcggt
    15841 tgatccacca aaccatgcct gcgccaatca ccagtgcaat ccccagcaaa atggcgctgg
    15901 cccataaaat tcgccgctcg ctacgcttaa tgaccggagc catcgccgcg ttcggtttgc
    15961 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    16021 ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac tgaccatgca
    16081 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    16141 cgtacaatac tttaccctgc gaatcggtca tatagacatg atattcattg cgcactttgt
    16201 taatgccacc gatattggcg cgaaacggac gatgttgtag ctgattaaac gcctgcgcca
    16261 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    16321 ttgcggtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    16381 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    16441 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    16501 aatcggtgaa agatcggggt tgatggcacg cagcttggca tgcaacgttt taatgtgggt
    16561 atcaacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca tcagttgctg
    16621 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    16681 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    16741 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    16801 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    16861 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    16921 taacgccgga tggagcgcca gtaactggct gcacaattca aagccgctga tatcaggcaa
    16981 accaacgtcg agaatcatga cgtcgggaac cagctggcga gctttatcca gcaccggcaa
    17041 gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    17101 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgtataa atcgcctctg
    17161 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    17221 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    17281 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    17341 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatagc
    17401 cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg ttttcgcccg
    17461 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    17521 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    17581 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    17641 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    17701 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    17761 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    17821 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    17881 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    17941 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    18001 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    18061 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    18121 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    18181 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    18241 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    18301 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    18361 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    18421 gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg ctatgtactg
    18481 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    18541 ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc aatgctcaac
    18601 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga cgccaaagcg
    18661 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg ttaacgctgc
    18721 agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacgacccag
    18781 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    18841 cgtaagcggc gcgctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    18901 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    18961 agtgccgcat tgacgcgacc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    19021 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    19081 atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    19141 tcacagccgc aggcctgggc gatgatttcc gccgtacgct gggtgcgtcc taaatcgcta
    19201 ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    19261 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    19321 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    19381 taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc
    19441 ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    19501 atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc agtgaggaaa
    19561 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    19621 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    19681 aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct gcggtgattc
    19741 tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat accggtattg
    19801 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    19861 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat gccgtgtatc
    19921 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    19981 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    20041 tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    20101 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc gccacgcaac
    20161 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    20221 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    20281 cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    20341 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    20401 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    20461 tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa gccagcacgt
    20521 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    20581 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    20641 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    20701 caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    20761 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    20821 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    20881 agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg taagccagcg
    20941 ggaatcgctc ttccagatga tcccaaagct tcccggcgct cgttgcctga acgctaagat
    21001 cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt tttgacttgc
    21061 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    21121 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    21181 tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc gggtagaaac
    21241 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    21301 gcaataaatc cgcctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    21361 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt cgcacacggc
    21421 gttcaatcaa cgacgtggat tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct
    21481 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    21541 gatcttcatt aagctgctgc gcctgggcaa gcgacgggat catcagccgt gcattttcag
    21601 catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta aaatcggtcg
    21661 ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    21721 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    21781 tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga tcttgtttac
    21841 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    21901 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    21961 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    22021 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    22081 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    22141 gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    22201 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    22261 aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    22321 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    22381 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    22441 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    22501 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    22561 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    22621 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    22681 aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca ttatggcggg
    22741 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttacct
    22801 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg aagaagcggt
    22861 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    22921 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga tcattcaggc
    22981 tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    23041 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tagcgttgtg
    23101 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    23161 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca ccgtggtggc
    23221 gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aacttgaccg
    23281 cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga aagatcagcg
    23341 cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    23401 gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc tggcacgctt
    23461 tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac tgtttattcc
    23521 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    23581 tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa tcgtcgggat
    23641 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg gtcaggaaca
    23701 gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcatcgg ttgatcagtt
    23761 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    23821 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    23881 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    23941 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    24001 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    24061 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacacaca tcctggacta
    24121 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg aagagtacaa
    24181 gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca agcgtattgc
    24241 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    24301 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    24361 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaat agccttagta
    24421 tccgcaattt cgtagcgggt cacccgtgct gattctttta aaccttgttt aatctcgaca
    24481 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    24541 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    24601 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    24661 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    24721 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    24781 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    24841 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    24901 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    24961 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    25021 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    25081 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    25141 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    25201 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    25261 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    25321 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    25381 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    25441 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    25501 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    25561 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    25621 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    25681 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    25741 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    25801 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    25861 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    25921 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    25981 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    26041 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    26101 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    26161 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    26221 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    26281 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    26341 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    26401 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    26461 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    26521 tttggttacg tgaccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    26581 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    26641 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    26701 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    26761 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    26821 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    26881 gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta cgcggtcgaa
    26941 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    27001 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    27061 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    27121 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    27181 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    27241 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    27301 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    27361 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    27421 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    27481 gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    27541 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    27601 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    27661 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    27721 cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    27781 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    27841 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    27901 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    27961 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    28021 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    28081 cagcaaccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    28141 tgaaagaggc agaccaggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    28201 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    28261 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    28321 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    28381 gtggcgctga tgcagggaac gtcatggttt agtcaaaacc accagcgaca acgtaatcca
    28441 cagctggaag aactgacccg ccccctggcg cgtcaggtaa cgctgaacgt tgcaccgctg
    28501 atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    28561 gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcacgttct
    28621 ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc gggcggctat
    28681 tttaaccagc agattgtcga accaattgcc gggaaaaacg gaccgctcgg ctatctgcgc
    28741 ctgacgctcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    28801 attttacgcc tgatgttgct gctttcactg gcaatcggcg tagtgctgac ccgcacgctg
    28861 ctacagggta aacgtaccct ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    28921 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    28981 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    29041 gtggaggagt gtatttttcg ccagatgccc gccacgcagc gcgttctgtt tctctggcgc
    29101 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    29161 cggatggaag aagataacgt ccgcctggca cggcgcagta gcggtggcgg cgcggtgttc
    29221 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    29281 atctccacgt cgattgtgct caatgcgctg aacgcgcttg gcgtcagcgc cgaagcgtcc
    29341 gggcgtaacg atctggtggt gaaaaccgcc gaaggcgacc gcaaagtctc aggatcggcc
    29401 tatcgcgaaa ccaaagatcg tggcttccac cacggcacct tgctgctcaa tgccgacctt
    29461 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    29521 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    29581 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    29641 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaatctt tgcccgtcag
    29701 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    29761 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    29821 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    29881 ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    29941 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    30001 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    30061 gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    30121 taaattatcg cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    30181 gatcgaaaag acggaacggt ttagctcaaa aatcaaaagc aaaatggcag aaatttatgg
    30241 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    30301 ttcgtgaata gatacttgaa tagattttat tttctatcca tatgaaaaat aatagtaaat
    30361 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc tcccactccg
    30421 gcttcacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    30481 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    30541 tgctcgtgag tgcggatgtg gtcagacacg gtacagatgg tcagggcttt cgcgccaaat
    30601 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    30661 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    30721 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    30781 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    30841 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    30901 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    30961 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttgtaa
    31021 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    31081 gtttcagcaa tatacttcgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    31141 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc aacatgtttt
    31201 caccctctcc ctgtgggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    31261 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    31321 atccgcgaag gtttcacggt gacccagtga acccggtttt actttcgggc cgtaaaccag
    31381 taccggaatg tgttcacgcg tgtggtcagt accggtccag gtcggatcac aaccgtggtc
    31441 agcagtgagg atcaggatgt cgtcatcgcg cagcaaagac atcagctccg gcagacggcg
    31501 gtcgaacagc tccagacccg cagcataacc ggcgacgtcg cgacggtggc cccaggaaga
    31561 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    31621 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    31681 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    31741 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    31801 acgctggaag ttaccggctt tgtcaccgat aaacggacga gcgataacac gaccgatgtt
    31861 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccag
    31921 gccaaaggtt tcttcgtggc aggcaatctg gaacacggag tcagcggagg tatagaaaat
    31981 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    32041 gtggcagtta ccgaggtaac ccggcagatt agcgcgttcg accagtttat ccagcagctc
    32101 ttgcgggaag ctgttttcgt gatcggagaa gtatccccac tcaaacagaa ccgggacacc
    32161 ggcgatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    32221 gtacgcgccg ataacttcag cgttgccgtc cattcccgcc ggaatgaaac cggtagaacc
    32281 ttcgtgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg ggcctttacg
    32341 accgttatca gcttcgcctt tggcacaagc ttctgcgata tgacccaggg tgtcagcccc
    32401 gacgtcacca aagcgttcag catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    32461 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa tgatcagatc
    32521 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    32581 ggcaagttta attgccgctt tcaccgcttt cgccgcttcc tgccagctgt tttcgtcttt
    32641 cgcgtggata accgccagcg gacgctgacc gtctacttgg tcgcccagac gcgccatatc
    32701 agtaaagccg acgctgtaat cgatggtgtc agatgcctga cgacgaccgc cgcccattgc
    32761 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    32821 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    32881 ggtcgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    32941 cagcaccgcc tgcaatttcg cgcgcgcttc agcgtcatct ttcgccagtt tgccggagat
    33001 cagcatctcc acgcacagcg ccatcgtgac atcaaacaga cgcgggttac gatattcacc
    33061 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    33121 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    33181 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    33241 tttcacgtcc atcaccagcg catccagacc ttccgccagt ttcttcgcca ggatcgaggc
    33301 agtgatcagc gggatggagt ccacggttgc ggtaatatca cgggtcgcgt agaaacgttt
    33361 atccgccgga gccagtgagc tggtctgacc gataatcgcc acgccgacgt ctttaataat
    33421 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    33481 gagcgtaccg ccagtatgac cgaggccgcg accggagatc atcggaatat agccgccgca
    33541 ggctgcgacc atcggcccca acatcagcga agtgacatcg ccgacgccgc cggtggaatg
    33601 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    33661 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    33721 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatgc cgttgataaa
    33781 gaaacgaatt tcttcatcgt tcagcgcatg accatcacgt tttttacgaa taatttcttg
    33841 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    33901 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    33961 aggcgtaaag catcttgctt agtagctgct ggcgctctta ccgtcaccgt ggcccagcgc
    34021 tttcaacagg cttgccagca ggctggaagc accaaagcgg tagtgacgcg catctgccca
    34081 gtcagcaccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    34141 cacgccgccc gccggtttaa aaccaacggt tttttctacg cccatatcac ggatcacttc
    34201 catcatgatg cgcgcgcttt ccggcgtcgc gttcactgcc actttaccgg tagaggtttt
    34261 gatgaagtca gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    34321 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    34381 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcgcggta
    34441 cgggaacaca acgtcaactt catcagcacc gtaggcgatt gccgcacggg tttctgccag
    34501 cgcgatttcg atgtcatcgt taccgtgtgg gaagttggtt accgtagcga ttcggatttc
    34561 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    34621 gatagcggcg gtattgccga ccggagtttt ggtctgatga cacagagcaa ttactttctc
    34681 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    34741 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    34801 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    34861 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    34921 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    34981 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    35041 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    35101 attttctggc tctggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    35161 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    35221 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cg
//
LOCUS       DAJTMK010000021        34953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTMK010000021 DAJTMK010000000
VERSION     DAJTMK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34953
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.021"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(234..1493)
                     /locus_tag="OO135_000880"
     CDS             complement(234..1493)
                     /locus_tag="OO135_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5396821.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEVTFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALSKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(1682..2545)
                     /locus_tag="OO135_000881"
     CDS             complement(1682..2545)
                     /locus_tag="OO135_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5396822.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            2672..3388
                     /gene="rph"
                     /locus_tag="OO135_000882"
     CDS             2672..3388
                     /gene="rph"
                     /locus_tag="OO135_000882"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5396823.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            3454..4095
                     /gene="pyrE"
                     /locus_tag="OO135_000883"
     CDS             3454..4095
                     /gene="pyrE"
                     /locus_tag="OO135_000883"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5396824.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(4132..4728)
                     /gene="slmA"
                     /locus_tag="OO135_000884"
     CDS             complement(4132..4728)
                     /gene="slmA"
                     /locus_tag="OO135_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5396825.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(4835..5293)
                     /gene="dut"
                     /locus_tag="OO135_000885"
     CDS             complement(4835..5293)
                     /gene="dut"
                     /locus_tag="OO135_000885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5396826.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(5271..6491)
                     /gene="coaBC"
                     /locus_tag="OO135_000886"
     CDS             complement(5271..6491)
                     /gene="coaBC"
                     /locus_tag="OO135_000886"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5396827.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            6663..7331
                     /gene="radC"
                     /locus_tag="OO135_000887"
     CDS             6663..7331
                     /gene="radC"
                     /locus_tag="OO135_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5396828.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7548..7784
                     /gene="rpmB"
                     /locus_tag="OO135_000888"
     CDS             7548..7784
                     /gene="rpmB"
                     /locus_tag="OO135_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5396829.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            7805..7972
                     /gene="rpmG"
                     /locus_tag="OO135_000889"
     CDS             7805..7972
                     /gene="rpmG"
                     /locus_tag="OO135_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5396830.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            8070..8879
                     /gene="mutM"
                     /locus_tag="OO135_000890"
     CDS             8070..8879
                     /gene="mutM"
                     /locus_tag="OO135_000890"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5396831.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(8918..9397)
                     /gene="coaD"
                     /locus_tag="OO135_000891"
     CDS             complement(8918..9397)
                     /gene="coaD"
                     /locus_tag="OO135_000891"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5396832.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(9405..10682)
                     /gene="waaA"
                     /locus_tag="OO135_000892"
     CDS             complement(9405..10682)
                     /gene="waaA"
                     /locus_tag="OO135_000892"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5396833.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     LLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            11095..12153
                     /gene="rfaQ"
                     /locus_tag="OO135_000893"
     CDS             11095..12153
                     /gene="rfaQ"
                     /locus_tag="OO135_000893"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5396834.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            12150..13274
                     /locus_tag="OO135_000894"
     CDS             12150..13274
                     /locus_tag="OO135_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5396835.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            13267..14064
                     /gene="rfaP"
                     /locus_tag="OO135_000895"
     CDS             13267..14064
                     /gene="rfaP"
                     /locus_tag="OO135_000895"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5396836.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            14080..15096
                     /gene="waaO"
                     /locus_tag="OO135_000896"
                     /gene_synonym="rfaI"
     CDS             14080..15096
                     /gene="waaO"
                     /locus_tag="OO135_000896"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5396837.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            15113..16108
                     /locus_tag="OO135_000897"
     CDS             15113..16108
                     /locus_tag="OO135_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR5396838.1"
                     /translation="MNEFIKERFSYLADNKKENVPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            16118..16810
                     /gene="rfaY"
                     /locus_tag="OO135_000898"
     CDS             16118..16810
                     /gene="rfaY"
                     /locus_tag="OO135_000898"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5396839.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            16836..17861
                     /locus_tag="OO135_000899"
     CDS             16836..17861
                     /locus_tag="OO135_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR5396840.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQDSCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            17946..18929
                     /locus_tag="OO135_000900"
     CDS             17946..18929
                     /locus_tag="OO135_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5396841.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNTC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRLVRLVNRKKNKR"
     gene            18975..20228
                     /locus_tag="OO135_000901"
     CDS             18975..20228
                     /locus_tag="OO135_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5396842.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(20298..21278)
                     /gene="rfaC"
                     /locus_tag="OO135_000902"
     CDS             complement(20298..21278)
                     /gene="rfaC"
                     /locus_tag="OO135_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5396843.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(21282..22328)
                     /gene="rfaF"
                     /locus_tag="OO135_000903"
     CDS             complement(21282..22328)
                     /gene="rfaF"
                     /locus_tag="OO135_000903"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5396844.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(22338..23270)
                     /gene="rfaD"
                     /locus_tag="OO135_000904"
     CDS             complement(22338..23270)
                     /gene="rfaD"
                     /locus_tag="OO135_000904"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5396845.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            23484..24680
                     /gene="kbl"
                     /locus_tag="OO135_000905"
     CDS             23484..24680
                     /gene="kbl"
                     /locus_tag="OO135_000905"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5396846.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            24690..25715
                     /gene="tdh"
                     /locus_tag="OO135_000906"
     CDS             24690..25715
                     /gene="tdh"
                     /locus_tag="OO135_000906"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5396847.1"
                     /translation="MKALSKLKAEEGIWMADVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            25954..26988
                     /gene="waaH"
                     /locus_tag="OO135_000907"
     CDS             25954..26988
                     /gene="waaH"
                     /locus_tag="OO135_000907"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR5396848.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(26975..27934)
                     /locus_tag="OO135_000908"
     CDS             complement(26975..27934)
                     /locus_tag="OO135_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5396849.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAAGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(27938..29221)
                     /gene="envC"
                     /locus_tag="OO135_000909"
     CDS             complement(27938..29221)
                     /gene="envC"
                     /locus_tag="OO135_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5396850.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADV
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(29231..30775)
                     /gene="gpmM"
                     /locus_tag="OO135_000910"
                     /gene_synonym="pgmI"
     CDS             complement(29231..30775)
                     /gene="gpmM"
                     /locus_tag="OO135_000910"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5396851.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            31020..31451
                     /locus_tag="OO135_000911"
     CDS             31020..31451
                     /locus_tag="OO135_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5396852.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            31593..31844
                     /gene="grxC"
                     /locus_tag="OO135_000912"
     CDS             31593..31844
                     /gene="grxC"
                     /locus_tag="OO135_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5396853.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            31907..32374
                     /gene="secB"
                     /locus_tag="OO135_000913"
     CDS             31907..32374
                     /gene="secB"
                     /locus_tag="OO135_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5396854.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTATLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            32374..33393
                     /gene="gpsA"
                     /locus_tag="OO135_000914"
     CDS             32374..33393
                     /gene="gpsA"
                     /locus_tag="OO135_000914"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5396855.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            33473..34294
                     /gene="cysE"
                     /locus_tag="OO135_000915"
     CDS             33473..34294
                     /gene="cysE"
                     /locus_tag="OO135_000915"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5396856.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(34347..34820)
                     /gene="trmL"
                     /locus_tag="OO135_000916"
     CDS             complement(34347..34820)
                     /gene="trmL"
                     /locus_tag="OO135_000916"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5396857.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         8746 a         8289 c         8721 g         9197 t
ORIGIN      
        1 gccgtcgtag ccgcaaaggt acgcgctcaa atatatagat tctgccgccg ccataatcaa
       61 aagatcatca atatctattt ctaataattc ggctgctctg ttaagcctga aaaaaggggc
      121 gggtggtaat tttttcattg cgtcaccttt tacgctgcgc cttattttgt gattgagcca
      181 ggcggtaagg cttcccgctt ttcgccccgt cgagctaggc tcaatctatt cagttagttc
      241 gcccttctga gaggcgttac gttgtagtct tttccgcttt ccagtgccac cagcagatca
      301 gcccactgtg ccagtgctgc tttacgctca tcaaaatact gatggcggtt ataaatcccc
      361 tctacgccct taatgcgatg attgaggcat cgttccgcca caacaggatc aacacccaac
      421 gctgctaaat gagtacgtgc ggtgcgtcga aagtcgtgaa tcgtgaaatt aggcacatcc
      481 ggcatttcgg cgcgaacctt agacagtgct acgggtaaag tgctttcctg aatatgtggg
      541 atcattctgt tttgcatctt cctggccgga agcacccatg cgctattaca tgaaaaggtg
      601 tgtagctctc tcaaccattc aacggctggg gaaggtagag gtatatcaat agggtctccg
      661 tttttgctgc gttcttccgg caaatgccat accgcaccat ctaaatcaaa ctcttcccat
      721 cgtgcggcgc ataattccat tttgcggacg cataacgcta gcagtaattt gaacgtgact
      781 tcgttctggc gactgaatcc cttagccgtg cgcatggctt tgaatagctg gattaactca
      841 tcgcgggtta accagcggtc acgggcagct tctttccctc cggcgtctgc cacctcaaag
      901 gctgaacagg ggttaatctc tagcgcgtgc cgtttgattc cgtagtcgaa tatgcggcgc
      961 gtccatctca gcacgtccgt tgctatggtc ggcgctccac ggtcaacaat acctttcagc
     1021 atgtcatcga tatggcgcgg tttcacgtcc tctaccttca tgctgccaat gcaggggttt
     1081 atatctttgt cgatacggcg gcggagtata tcggggtgct tccagcgcgg gaggatctga
     1141 cgctcaaagt attcagcggc aagctctgaa acgcgcatag cgttcttctc tgcttccatc
     1201 ttcgccagtg cttcggtttt gcgcttctgc ttctctcctg caacgtcata gcccagcgca
     1261 acgcgagccg atagctcttt ggctgtctct ctggcctttg atagtgatag ctcgctatac
     1321 gaaccaatta gcatggcgcg ggacttccct gcgagtttat agcggaatct ccatgtgggg
     1381 gttttgtcgg cttcacggta acgtaggtat aaaccgttac cgtctgcccg accctcgaat
     1441 cgctctccgc tcttaatcca tgcgcggatc tgcatgtctg ttagttttgg cattttgcga
     1501 atatccaaac tttgaatagg gggtgtacac aaaggtgtac ccaaacctca ttgggtacac
     1561 ctttgtgtac accataatca tgcgctttgt tgcgtcttta tgatagccca tgataacaaa
     1621 aaagcccgct gggttgcggg cttggtgtga tttatgcgtc gtcctaaatg ttgctgatag
     1681 cttactcgat attctgaatc tgctcgcgca tctgctcaat caacactttc agctcaatcg
     1741 cggagtttgt cacttcggca ttgatagact tcgacgcaag agtgttcgac tcgcggttga
     1801 attcctgcat cataaagtcc agacgacgac caacggcttc ttttttcttc agaatgttgt
     1861 aggtctcttt gacatgcgct tccaggcggt ccagttcttc ggcaacgtca attcgttgtg
     1921 ccagcagaac cagctcctgc tccagacggt tgttttccaa ctgaacctga gcatcttcca
     1981 gcttcgcgac cagacgctca cgctgccatt gcaggatttc cggcatatgg gcgcggactt
     2041 tgaccacttc ggcggtgacg ccttccagac gctgctcgat caatgctttc agtgcctgac
     2101 cttcggtttc acgcgcgaca ataaagtcgt ccagcgtacc atccagcgcc gcgagaattt
     2161 cagcggcaat ggcgtcaaga tcctgctcct gggctgccat cacgcccggc cagcgtagaa
     2221 tatcaaccgg gttgatttcc ccttcgtcac tctgcatttt tacccagttc gcggcagtta
     2281 ccagctgttt agccagtttt tcgttgagga tcagctcacc ttgcgcgcta atatctggct
     2341 catagcgcag ggtacattcc actttaccgc gcgtcaggcg agaacgaata cgctcgcgaa
     2401 cgacaggttc aaggctacgg aactgctccg gcagacgaaa gtaagtttcc agataacgct
     2461 ggtttaccga gcgcatttcc caggttgcgc tcccccattc acccttgatt tcacgccggg
     2521 cgtaggcggt catactgcgg atcatagacg ttcctgttta taaaaggaga ggtggaagga
     2581 ttatagccat cgatgccttg tcaggatagg aataaccgcc ggaagtccgt ataatgcgca
     2641 gccacatttg tttcaagccg gagatttcaa tatgcgtcca gcaggccgta gcaataatca
     2701 ggtgcgtccc gttaccctga ctcgtaacta tacaaaacat gcagaaggct cggtgctggt
     2761 cgaatttggc gataccaaag tgttgtgtac cgcctctatt gaagaaggcg tgccgcgctt
     2821 cctgaaaggt cagggccagg gctggatcac cgcagagtac ggcatgctgc cacgttctac
     2881 ccacacccgt aacgctcgtg aagcggcgaa aggtaagcag ggtggacgca caatggaaat
     2941 ccagcgtctg atcgcccgtg ctcttcgcgc ggcagtagat ttgaaagcgc tgggtgagtt
     3001 caccattacg ctggactgcg acgtgcttca ggctgatggt ggcacgcgta ccgcgtcgat
     3061 tacgggtgcc tgcgtggcgc tggcagatgc gctacagaag ctggtggaaa acggcaagct
     3121 gaaaaccaac ccgatgaaag ggatggtagc cgcagtttct gtcggaattg tgaatggcga
     3181 agcggtttgc gatctggaat acgttgaaga ctctgccgca gagaccgaca tgaacgtagt
     3241 gatgaccgaa gacgggcgca tcattgaagt gcaggggacg gcagaaggcg agccgttcac
     3301 ccatgaagag ctactcacct tgttggctct ggcccgaggg ggaatcgaat ccattgtagc
     3361 gacgcagaag gcggcgctgg caaactgatt tttaaggcga ctgatgagtc gccttttttt
     3421 tgtctgtaga aaagtaagat gaggagcgaa ggcatgaaac catatcagcg ccagtttatt
     3481 gaatttgcgc ttggcaagca ggtgttaaag tttggcgagt ttacgttgaa gtccgggcgc
     3541 aaaagcccct atttcttcaa cgccgggctg tttaataccg ggcgcgatct ggcactgtta
     3601 ggccgttttt acgctgaagc gttggtggat tccggccttg agttcgatct gctgtttggc
     3661 cctgcttaca aagggatccc gattgccacc actaccgccg tggcgctggc ggagcatcat
     3721 gaccttgacc tgccgtactg ctttaaccgc aaagaggcaa aagaccacgg tgaaggcggc
     3781 aatctggttg gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt gatcaccgcc
     3841 ggaacggcga ttcgcgaatc gatggagatt attcaggcta atggcgcgac gcttgctggc
     3901 gtgttgattt cgcttgatcg tcaggaacgg gggcgcggcg agatttcggc gattcaggaa
     3961 gttgagcgtg attacaactg caaagtgatc tctatcatca ccctgaaaga cctgattgct
     4021 tacctggaag agaagccgga aatggcggaa catctggcgg cggttaaggc ctatcgcgaa
     4081 gagtttggcg tttaaagaaa ctcgccggat gaaaagtcat ccggcgtcat attactgcaa
     4141 ctgtgccgca attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt atttaaattc
     4201 gctgcggaca aaacgtgaca gcataccttc acagaaggcc aggatctggc ttgccagcag
     4261 ggtttcatcg gtggtgtaac cttcaccctc acgcattctc ttttcacgca atacctgacg
     4321 cagctgcgct tcaatacgct cgaacagctg gttgatgcgc ccttgcaggc gatcctgttc
     4381 aaacattagc gcatgaccag tgaggatgcg ggtcaggcca ggattacgct caccaaaacc
     4441 gagaagcagc aacacaatca gacgcaggcg cgctgtggtg tctttctcat ctttcagaat
     4501 caggttgatg cgagtaatca ggctatcttc gataaactca atcaggctat cgaacatgcg
     4561 ggtcttactg gggaagtggc gatacagtgc cgcttcggaa acgccgacag aggcggccag
     4621 ttttgccgtc gtgatacgtt ggcttccatc gctggattcc agcatcagcg ccagagactg
     4681 aagtatttcc tcgcgacggt tccttttcgc agtttgtttt tctgccatgt tacaaaatac
     4741 ccctgaaaat aagcacttgc caggcggcac ccacgctatg accgcaaacg aaatgtttgc
     4801 ggctatgtta tgacgttatt cggatgcgta tgtgttactg acgaccagag tgaccaaagc
     4861 cgccttcacc gcggtcggtg gcgtcgaaat cttccaccag attaaattca gcctgtacta
     4921 ccggaacaaa aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag ctgtcctgac
     4981 cacggttcca cacggaaatc atcaactggc cctgatagtc ggaatcgatc aatcctacca
     5041 ggttaccaag cacgataccg tgcttatgtc ccaatccgga gcgcggcagc atcattgccg
     5101 ccagtgaagg atcggcaata tgaatcgcca gcccggtcgg aaccagcgta gtgtcacccg
     5161 gagccagttc tacggcgtcg tcgagacagg cacgcaggtc aagtccggca gagccagagg
     5221 tggcataagt cgggagcgga aattccttcc caacgcgcgg gtccagaatc ttaacgtcga
     5281 tttttttcat cataacgggt cacgatctcg tcgagtaata gttggccaag gagctctttg
     5341 cgctcaagcg gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc gttgttgtcg
     5401 ctgttaaatc cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc aaggttttta
     5461 cggatacgtt tttgccgggc gtattcttcc acattatttg tttcggcggc aaatccaacg
     5521 acgtagggtc gatggtcttt tagtgcggca acgcctgcga cgatatcggg gtttttaacc
     5581 atttttattg ttaattcatc accctgcgtg gcctgctttt tgattttttc tggggccacg
     5641 gtagctgcgc gataatccgc cacggcggcg cagccgataa aaatattttg ctgctgtaca
     5701 gaagcattca cggcggcttc catttccagc gcggtcatca catcaacacg tttaacaaac
     5761 ggtggcgtcg gtagtgaaac cggacctgat accagcgtga cgttcgcgcc acgacgggca
     5821 gcggcggcgg cgatagcaaa acccatcttg ccggagctgt gattagagat ataacgcacg
     5881 ggatcgagcg gttcacgcgt cggaccggcg gtaatcataa tgttcagatg tttcaggtcg
     5941 ttgacgggcg aaaaatgcgc caccgccata tccacaatgg ttaacggatc gagcattcgc
     6001 ccaggaccga tatcaccaca agcctgactg ccactgtctg gcccccagat gagcaaacca
     6061 cgggaagcaa gcccctctaa attatgctgc gtggcagcgg cacggtacat ctgctggttc
     6121 atggcgggga gcacggctac aggcgcaggt gtagccagac aaatcgtcga taccaggtca
     6181 ttcgccattc cggcagcaac acgggcaatc aaatctgccg tggcaggggc gagaatcact
     6241 aaatcagccc atttacccag ctcaatatgg cccatagcgg cttcggctgc cgggtccagc
     6301 aaactgtcgg aaacgggata accagaaacc gcctgcaagc taagtggggt gataaaggct
     6361 tttgccgctt cggtcatggc tacgcggacg tcggccccgc gatcgcgcaa acgacgcacc
     6421 agttcagggg ttttataggc agcaataccg ccgctaacgc cgagaacgat ttttttaccg
     6481 gccaggctca tgatgatttt tcctgttggg tgacaccaga agttggcgat tttatcacaa
     6541 tacttttgtt gtcgtgcctt cacctgagaa tcactttgcg aggcgctttc caggattgaa
     6601 aactggccgt cgatttaacg gaacggctat gacaggatgc gagcaccaca aaggaggtga
     6661 aggtgaaaaa caattcacag ctgttgatgc cgcgcgaaaa aatgctgaag tttggtatta
     6721 gcgccttaac ggatgtcgag ctgctggcgc tatttctgcg taccggaacg cgcggtaaag
     6781 atgtattaac cctggcaaaa gagatgctgg agaatttcgg ctctctttat ggcttgttaa
     6841 cctctgaata tgaacaattt agtggcgttc atggaattgg cgtggcgaaa tttgcccagt
     6901 taaaggggat tgctgaactg gcgcggcgtt actacaacgt gcggatgcgt gaagaaagcc
     6961 ctttactcag cccggagatg acgcgggaat ttttacaaag ccagcttacg ggtgaggagc
     7021 gggagatctt tatggtgatc tttctcgact cacaacaccg ggttataacg catagccgtc
     7081 ttttttccgg cacgctaaac catgttgaag tccatcctcg ggaaattatc cgcgaagcga
     7141 taaaaataaa cgcctcggcg ctgatccttg cacataatca cccttcgggt tgtgctgaac
     7201 ccagtaaagc ggataaactc attactgaac ggataataaa gagttgtcag ttcatggatt
     7261 tacgcgtgct cgaccatatc gtgattgggc gtggagagta tgtttctttt gctgaacgcg
     7321 gctggattta acccgctatg cgcgatcctt cgggatcttt gtctgttcgg gacttgagca
     7381 catcgctgag tcagcgtata ctacgccacc tttgagaatc tcgggtttgg catttgggcc
     7441 tggcaatcga gagttcacat agaactgcga tgactgggct gtaaagcctg acgaggcgcc
     7501 aataccccat acgaagctcg agctaatttg atttttggag aatagacatg tcccgagtct
     7561 gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca ctgaacgcga
     7621 ctaaacgccg tttcctgccg aacctgcact ctcaccgttt ctgggttgag agcgagaagc
     7681 gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa aaaggcatcg
     7741 atacagttct ggctgaactg cgtgcccgtg gcgaaaagta ctaagtactt agaggaaata
     7801 aatcatggct aaaggtattc gtgagaaaat caagctggtt tcttctgctg gtactggtca
     7861 cttctatacc actacgaaga acaaacgtac taagccggaa aaactggaac tgaaaaaatt
     7921 cgatccagtt gttcgccagc acgtgatcta caaagaagcg aaaatcaaat aattctcgct
     7981 ttgatgtaac aaaaaacctc gctccggcgg ggttttttgt tatctgcttg cccccatatt
     8041 gactgcatct gttcattcct ggagatgcta tgcctgaatt acccgaagtt gaaaccagcc
     8101 gccgcggcat agaaccgcat ctcgttggtg caaccattct tcatgcagtg gtgcgcaacg
     8161 gacgcttgcg ctggccggtt tcagaagaga tctaccgttt aagcgaccaa ccagtgctta
     8221 gcgtgcagcg gcgggctaaa tatctgctgc tggagctgcc tgagggctgg attatcattc
     8281 atttagggat gtctggcagc ctgcgcatcc ttccagaaga acttccccct gaaaagcatg
     8341 accatgtgga tttggtgatg agcaacggca aagtgctgcg ctacaccgat ccgcgccgct
     8401 ttggtgcctg gctgtggacc aaagagctgg aagggcataa tgtgctggcc catcttggac
     8461 cggagccgct tagcgacgat ttcaatggtg agtatctgca tcagaagtgc gcgaagaaaa
     8521 aaacggcgat taaaccgtgg ctgatggata acaagctggt ggtaggggta gggaatatct
     8581 atgccagcga atcactgttt gcggcgggga tccatccgga tcggctggcg tcatcactgt
     8641 cgctggcaga gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg cgttcgattg
     8701 agcagggtgg tacaacgctg aaagattttc tgcaaagtga tggtaaaccg ggctatttcg
     8761 ctcaggaatt gcaggtttac gggcgaaaag gtgagccgtg tcgggtgtgc ggtacgccga
     8821 ttgtggcgac taaacatgcg cagcgggcaa cgttttattg tcggcagtgc cagaagtaat
     8881 tcatgcacgc cggatcgcat accatccggc ataaacgcta cgctaacttc gccatcagcg
     8941 cctgatggac attctccggc aggaaatggg tgacatcgcc ctgatggcgc gccacctctt
     9001 tcaccaacga tgaagagata aacgaccact ctttcgacgg catcagaaac acactttcca
     9061 gttccggcat taagtggcga ttcatatgcg ccagctgcat ttcatattca aaatccgcca
     9121 ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt acgggcgaag ttcgccatca
     9181 aatcactaaa cccaactact tccacgttcc ccagatgcgc ggttgcctgc tgtgccagcg
     9241 ccacacgctc ttccagggta aacatcggtt ttttactggg gctggcggca atcgccagaa
     9301 taacgtgatc gaacatttgc gtggcgcgcg tcacgatatc gatatgacca ttggtaatgg
     9361 gatcgaaagt acccggataa atcgcccgtt tttgcataac aacctcaatg cgttttcggt
     9421 ggcaggtaag gttccagcag ttgaagcagg cgctgtagcg cgccctggtt ttgatacagt
     9481 acttcaacgg catgacggcc atagaaacta cggtaatcgg cgtcggtgag taaagaggaa
     9541 acctcttttg caagcgtagt ggcatcggta acggtaatca gcccgcttgc ctgctccagc
     9601 cgcgcgcaaa tgtctttaaa attaaaagta tgcggcccca tcaataccgg aatagcgtgt
     9661 gcggcagctt ccagcggatt atgcccacca cgttcaacca gtgaaccgcc aacaaaggcg
     9721 agatcggcaa tgccgtacag taacatcaac tcgcccatcg tatcgccaac cacaacctgc
     9781 gtgctggtgg aggggacttc ccctgaagag cgtgtgatat agcttagtcc agcctggcgg
     9841 acaaggttaa tcgcatccgg gaagcgttct ggatgacggg gtaccaggat gagcaataaa
     9901 ttcgggaatt gctgtaacaa tacctgatgt gcggcgatca ccacactctc ttcaccttcg
     9961 tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc actggcggcg cagcgtcacg
    10021 gctttagcag ccaactgcgg cgttacagaa atatcgaatt tcaggctacc ggtaacggtt
    10081 acctggttat tttttgcgcc cagcgccaca aaacgtgcac catcttcttc attttgggcg
    10141 gcaatcagcg taatacgacg cagcaagcga cggacgaatt tacccagttt ggcataacct
    10201 gcggccgagc gggcagagag tcgcgcgttc gcgatcacca gcggaatttt acgtttatgt
    10261 agcgccgcaa tcaggttagg ccatagttcg gtttccataa tcaacaccag tttagggtcg
    10321 actttattca ggaaacgatt aagtgcatcg ggcaggtcat acggcagata aacgtgctga
    10381 acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat ggttgtaacg
    10441 gtaatcggta aatcaggata acgatgacgc agcgcgcgca ccaacgggat cgccgccaga
    10501 gtttcaccga cggagacgga gtgcagcata atgccgcctg gttttagcag atggcggtaa
    10561 aaaccgtaac gttcacccca gcgttttcga taggccggag ccttacgtcc gcgcacccag
    10621 agccgtatcc agatcagcgg ctgaataagg tagagaaggg cggtgtaaag caattcgagc
    10681 atagtaaata gctgacttat ggatgtgctg gggattctat gtatttagct gtggctttac
    10741 cattactttt cccgtttttg acttaaatag caatttaccc gccaattctt gctgaacatc
    10801 tatacttttt ttgtattttt atgcgattca ttgatttctg ctgtacgtgc aggaaggaat
    10861 cgcagagcaa cagtgaaaga atatggttgc taatgatttt atttgattga tttctttatt
    10921 gaaaatcaac aatgtgctta aaaatcattc gggtcggatt gccgcgaaag aagtgataca
    10981 ctagcgcgtc aatatcagtg cggtatcagt attcaggtag ctgttgagcc tggggcggta
    11041 gcgtgctttt tctgcttcac cttaatcgga taatctcaac aaaaagagtt acttgtggat
    11101 aagctatttc gaagaatttt gctcattaag atgcgttttc atggggatat gttattaact
    11161 actcccgtca ttagttcgct gaaaaaaaat taccctgacg caaaaatcga tgtgctgctt
    11221 tatcaggaca ccatcccgat cctgtctgaa aatccagaga ttaacgcgct ctacggcata
    11281 aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact tttttcatct catcaaggta
    11341 ttacgtgcca ataagtatga ccttatcgtc aatctcaccg atcaatggat ggttgctata
    11401 ctggttcgct tattaaatgc ccgtgtgaaa atttcccagg attatcatca tcggcagtct
    11461 gctttttggc gtaaaagttt cacccatttg gtgccgttgc agggtggaaa tgtggtggaa
    11521 agtaacttat ccgtgctgac cccattggga gttgattcgt tggtgaagca gacaaccatg
    11581 agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcacgc tggtgttgga
    11641 caaaattatg tggttatcca acctacggcg cggcaaatct tcaaatgctg ggacaacgcc
    11701 aagttttccg ctgtgattga tgccttacat gctcgtggtt atgaagttgt tctgacgtcc
    11761 ggcccagata aagacgatct ggcctgcgtc aatgaaattg cgcagggatg ccagacgcca
    11821 ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt aatcgatcat
    11881 gcgcagctgt ttattggcgt tgattccgca ccggcgcata ttgccgctgc agttaatacg
    11941 ccgctgatat cgctgtttgg tgcgacagac catattttct ggcgtccctg gtcaaataac
    12001 atgattcaat tctgggcggg agattaccgg gaaatgccaa cgcgcgatca gcgtgaccga
    12061 aatgagatgt atctttcggt tattccggcg gcagatgtca ttgctgctgt cgataaatta
    12121 ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc tttttgttta tataaatatt
    12181 ttccctttgg cggtttgcag cgcgatttta tgcgtattgc tcagacagtc gccgcccgag
    12241 gtcatcatgt tcgggtttat acccagtcgt gggaaggcga ataccctgat gtatttgaac
    12301 tgatcaaagt gccggttaaa tcgcatacca atcacgggcg caatgcggag tattttgcct
    12361 gggtgcaaaa acatttacgc gaacatcccg tcgataaagt cgttggattc aacaaaatgc
    12421 cggggctgga cgtttattat gccgctgatg tttgttatgc cgagaaagtc gcgcaggaaa
    12481 aaggcttttt ctatcacctg acgtcacgtt atcgccatta tgccgccttt gagcgggcaa
    12541 ccttcgaaca gggcaagccg acacagctgc tgatgctgac agataagcaa atcgccgatt
    12601 tccagaaaca ttatcagact gaagcggagc gttttcatat tctgccaccg gggatttatc
    12661 ctgatcgtaa atatagccag cagccagcta atagccgtga aatcttccgt aagaagaatg
    12721 gaataaccga acaacaatat ttattgttgc aggtcggttc agacttcacg cgtaaaggtg
    12781 tcgatcgttc cattgaagca cttgcttcgt taccggattc gctgcgccac aacacattgc
    12841 tatatgttgt tggacaggat aaaccgcgaa aatttgaggc gctggcagaa aaacgcggcg
    12901 tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt ctcggaatta atggcggcgg
    12961 cggatttatt actgcatcct gcctaccagg aagcagcggg aattgtgcta ctggaagcga
    13021 ttactgcagg attaccggta ctaacaacag ccgtttgtgg ctatgcgcat tatattgtcg
    13081 acgccaattg cggcgaggct attgctgagc cattccgcca ggaaacattg aatgagattt
    13141 tacgcaaagc gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat gcgcgacatt
    13201 atgctgatac acaagattta tacagtctgc cagagaaagc ggcggatatc ataacgggtg
    13261 gtctggatgg ttgaacttaa agagccgttt gccacgttat ggcgcggtaa agatcctttt
    13321 gaggaagtta aaaccttgca gggtgaggtt tttcgtgaac tggaaactcg ccgcactctg
    13381 cgatttgaaa tggcgggcaa aagctatttt ctcaaatggc atcgcggcac gaccctgaaa
    13441 gagataatca aaaatttact ctcattgcgg atgccagtat taggcgcaga ccgcgaatgg
    13501 agtgcgattc atcgactgcg ggatgtcggc gttgatacta tgtatggggt ggcatttggc
    13561 gaaaaaggta ttaatccgct gagcagaacc tcgtttatta ttaccgaaga tctgacccca
    13621 accatcagtc tggaagatta ctgtgctgac tgggcgacca acccaccaga tgttcgcgta
    13681 aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc tgcgggcatt
    13741 aaccaccgtg actgttatat ctgtcatttc ctgctgcact tgcctttttc cggtaaggaa
    13801 gaggagttaa aaatttcggt aattgaccta caccgggcgc agcttcgcac gcgcgttcca
    13861 cgtcgttggc gcgataaaga tcttataggg ctttattttt cttcaatgaa tattggcctg
    13921 actcagcggg atatctggcg gtttatgaaa gtgtattttg ccgccccgct taaagacatt
    13981 ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa aatcagggaa
    14041 agaacgattc gaaaatcgtt gtaatttccg gagagaaata tgagtgccca ctattttaat
    14101 ccacaagaga tgatcaataa gacaatcatc ttcgatgaaa ggccagcggc gtcagtggca
    14161 tcatcattcc atgttgctta tggcattgat aaaaactttc tttttggttg tggtgtttca
    14221 atcacgtcag ttttgttaca taacaacgac gtgagttttg ttttccacgt ttttattgat
    14281 gatatccctg aagccgatat ccagcgttta gcccaattgg cgaaaagcta tcgtacctgt
    14341 atccagatcc atctagtaaa ttgtgaacgg cttaaggcat taccgacgac caaaaattgg
    14401 tctattgcca tgtatttccg ttttgtaatt gcagattact ttattgatca acaagataag
    14461 atcctttacc tggatgctga tatcgcctgt cagggaaact taaagccgct gataacaatg
    14521 gatcttgcca ataacgttgc tgctgttgtt actgaacgcg atgctaactg gtggtcgtta
    14581 cggggtcaaa gtctgcagtg taatgaactt gaaaagggct actttaattc aggtgtcctg
    14641 ttaattaata cactagcgtg ggcgcaggag tccgtttctg ctaaagcgat gtcgatgctt
    14701 gctgataaag ccatcgtttc ccgtttcacc tatatggatc aagatatcct taatcttatc
    14761 ctgttaggga aagttaaatt cattgatgct aaatacaata cgcaatttag tttaaattat
    14821 gaattaaaaa aatcatttgt ttgtccaatt aatgatgaaa ccgtattaat tcattatgtc
    14881 ggcccgacaa aaccctggca ttactgggcc ggttatccaa gtgcgcaacc ttttatcaaa
    14941 gccaaagaag catcgccctg gaaaaatgaa ccgttaatgc ggccagttaa ctcaaactat
    15001 gctcgttatt gcgccaagca taattttaaa caaaataaac caattaacgg gataatgaat
    15061 tatatttatt atttttattt aaagataata aaatgaaatt gagaaaagtt aaatgaatga
    15121 atttataaaa gaacggtttt cgtatttagc agataataaa aaagaaaacg tcccagagct
    15181 aaatgtttcc tacggtatcg ataagaattt tttgtatggt gctggcgttt caatttcttc
    15241 cgttttgatt aataattcag atattaattt tgtctttcat gttttcactg attatgtgga
    15301 tgatgattat ttaaagtcat ttaatgaaac agcaaaacaa tttaatacct caattattgt
    15361 atatttaatt gaccccaaat actttgctga tctgccgacg tcacagtttt ggtcatacgc
    15421 gacatacttc agggtattgt cttttgaata tctgagtgaa agtatttcca cactgctgta
    15481 tctggatgcc gatgttgttt gtaaaggaag cctgaaacct ctcacagaaa ttatatttaa
    15541 agatgagttt gctgcggtca ttcctgacaa tgatagtact caggcggcat gtgcaaaacg
    15601 cctcaacatt cccgaaatga atggacgtta tttcaatgca ggcgttatct atgtcaatct
    15661 taaaaaatgg catgaagcaa atttgacacc gtatttactc aaacttttac gaggggaaac
    15721 taaatatggc tctcttaaat atttagatca ggatgcgttg aatatcgcat ttaatatgaa
    15781 taatatctac cttgcgaagg attttgatac tatttatacc ctgaaaaacg aactttatga
    15841 tcgtagtcat cgaaagtatc agcaaaccat taccgataaa acagtgttga ttcactatac
    15901 agggataact aaaccatggc atagctgggc tggatatccg tctgcatcat actttaatat
    15961 cgcgcgtgaa caatctccct ggaagaaata tcctcttaaa gaggcgcgga ctgttgcaga
    16021 aatgcagaaa caatataagc atctgtttgc ccatggtgag tatataaaag gcataacttc
    16081 attaattaag tacaagctta agaaataaat tactcttatg attacaagta tacgttatcg
    16141 cggcttctca ttttattata aagataatga taataaatat aaagaaatct ttgatgaaat
    16201 actggcctat aattttaaaa cagtaaaagt attatgtaat attgatgata ccaaagtatc
    16261 gttgattgat acgaaatatg gtcgctatgt ttttaaagtc tttgctccga aaacgaaaag
    16321 gaatgaacgt tttttaaaat cttttgtaaa aggtgactac tatcagaatt tgatcgttga
    16381 aaccgatcgt gtcagaagtg caggtcttac ttttcctaat gatttttatt ttctggctga
    16441 gcgtaaaatt ttcaactatg ccagtgtttt tatcatgctt atcgagtatg tcgagggcgt
    16501 agagctcaat gatatgccga ttattccaga aaatgttaaa gcagaaatta aggcttcaat
    16561 ggaaaaattg catgccttaa atatgctatc gggcgaccca caccggggta actttatcgt
    16621 tagtaaagat ggtgtcagga taattgacct atccggtaaa agctgtacgg cagagcgtaa
    16681 agctcgtgat cgtctggcca tggaacgtca cctgggtatt gccaacgaaa taaaagatta
    16741 tggctactat tcagtaatct acagaaccaa actacgcaaa tttattaaaa aattaaaagg
    16801 caaagcgtaa accacacagt caaaacggaa ccaacatgga tttattagct gagagtatta
    16861 ctgaagtcgc tgtctctggg gaaattgcta acaccgatcg tgtgttaaat atcgcttacg
    16921 gtattgaccg taacttttta tttggtgcgg cagtatctat gcaatcagtt gttatgcata
    16981 acccggacct tgcggttaag tttcatctct ttaccgacta cattgatgaa gattatctac
    17041 aacgtgttaa tgcttttacc agcaaaaatg ctaacgttga agtaataatt tataaagtct
    17101 ccaatgcctt tattgatatc ttccccagcc tgaaacagtg gtcttatgca acattcttcc
    17161 gtttagttgc gttccagtat ttgagtgaaa ctattgaaaa tctgttatat atcgatgctg
    17221 atgtcatctg taaaggctca ttagctggat tgcttgatat taattttgat gaggataagt
    17281 tcgcagctgt tattaaagat gtgcctttta tgcaggaaaa accagcgaag cgtctggcta
    17341 tagagggact tccagggaat tatttcaacg ccggtgtagt atatctgcag cttgaagcat
    17401 gggcgaaaaa tgattttatg aataaagcca ttgctatgct ggcaagtgac ccgcagcaca
    17461 cgaagtataa atgccttgat caggatattt taaatattct gttctttggt cattgtaatt
    17521 ttattagcgg cgattatgat tgcttttatg gcattgacta tgagttaaaa aataaaagcg
    17581 atgaagatta taaaaagacc attaccgatg atactaagct gattcattat gttggcgtaa
    17641 cgaagccctg gaacgactgg acgaattatc cctgccagaa gtattttaat gaggcttatc
    17701 aggattcttg ctggaatgat gtggcgttta ttccagccac gaatgaaaag cagtatcaag
    17761 tgaaatatca acatgcaaag aaaaatggtg atacgtttaa cgcttttatt tacttcatta
    17821 aatttaaatt aaataagtat aaaagaaaac tatttggata ataataaaaa tcatggatgt
    17881 ctgagttaca ataataatta aggatgagta ctatcatatt ttattagttc aggcaagaga
    17941 acacgatgag caatgattac cctttagtat ccataataat accgacgtat aattcatctg
    18001 attacattac tgaaactcta acgaaattag aaaaacaaac ttacccaaat tttgaaattg
    18061 ttattgttaa tgatggttct aaagataaca catcaaacgt tttgagagag tatgggttaa
    18121 cccactctcg attaattatt atcaataaag aaaatggcgg tgtttcgtct gccaggaata
    18181 caggtatccg caaggcgcaa ggacagttta tatgttttat ggatgatgat gatgagatag
    18241 atcctaacta tctgctgaag atgtatacca gacaacatga gacgggaggg gatgcgattt
    18301 attgtgggct ttatggccat tatataaaag atggtgttac ttactcacct ataaatacag
    18361 agtttaatga aggatcttta cttttcgact ttttttataa aaaggttaga ttccatatag
    18421 ggtgcttgtt tataagaaaa caacttctgg aagagaataa tctttttttt gatgaagatt
    18481 tacgactagg agaagatctt gattttatct atcgactgct aattacatgc gatatgtatg
    18541 cggttccata ttatatgtat aagcataact atagagaaaa ttccttaatg aacacatgta
    18601 gaaccatcac tcattatcga catgagtcat ttgcgcacga aagaatctac tcttctgtga
    18661 tgcagttata taaaggtaac cggaaagaag aaattcatac attattgagt caaaatagag
    18721 cttatcataa aactcgttat ttatggaatg ttctacttaa tggtgatttt aaactattga
    18781 atcaattagt tgaaagcaat gaaaaagaat taagagattg taatcttcct ggcaagagag
    18841 ataagagacg agcaaaaata ttagcatcaa aaaattatat tctctggagg ttggtaagac
    18901 tggtaaatag aaaaaagaat aaacgttaga tcttgcatta aataacccat cctcgtagca
    18961 taggttgaaa ttatatgtcg ttttgttgga atgaaattaa ttctggtata aagtctttaa
    19021 ttctcatatt atgtattttt tctttaatga ctttgtcttt atgggatgat gttgcaacaa
    19081 agtttcttca tgcagctgga attatatctg cattgtattt tcttgcgaca ccaaaaaaaa
    19141 caataactaa taatcctact ttgttaattt tcatctcatt atgtcttttg ggtatcgtaa
    19201 atatcatctg gtattcacat tataaagttt caggctctgt ttataccaat gcatatcgtg
    19261 gcccaatgga aactggaaaa attgccttgt gtagcgcttt tattttctta gttctttttg
    19321 ctaaaaatga gatgagaaca aaaataaaat ttgggaaact aattctgttc gcatccctgg
    19381 caacgcagtt actttttttt gcgcatgcca tgtggcaaca tttctattta aacgtcgacc
    19441 gtgttgcatt atcagcttcc cacgctacaa cagcaggcta catcatcctt tttccttctt
    19501 tactggcatc aattctcatt ttaaaatccg actttagaca taaaacaaca ttatatacaa
    19561 ttaacttcat gcttagctta tgtgctgtca tagtaactga gacgcgtgca gccatattag
    19621 tgtttccatt ctttgcgtta atattaatcg taatggatag ttatattaat aagcgaatta
    19681 attataagtt atattgtttt attgcgattg cattattagc aggtgtattt tcttttaaag
    19741 atacattgct tatgagaatg aatgacttaa ataacgattt agttaattat tcgcatgata
    19801 acaccagaac ttcagtcggt gcccgtttgg caatgtatga agttggctta aaaacatatt
    19861 ctccaatagg acaatcactg gaaaaacgtg cagaaaaaat acatgagcta gaagaaaaag
    19921 agcctagatt gagtggcgct ttaccctttg tagattctca tttgcataac gatctcatag
    19981 atacgttatc aacgcgtggt attcctggag ttgtattaac aattttagca ttttcagcaa
    20041 tactcatata tgccttaaga actgctaaag aaccttatat tttaatcttg cttttttcac
    20101 tactggtagt aggcctaagt gatgtaatac tcttttctaa accggttccg actgctgtgt
    20161 ttgtcaccat aatattgctt tgtgcttatt ttaaagcaca atcagaccaa tatttattag
    20221 ataagtaatg aatatatgaa ataaaatcag caaggaggct gattttattt caaaattatc
    20281 tattaagggg ttgtaattta aatcatagca gcgttttcag cgttttcttc aatgaatcgc
    20341 ttaacagcat ttgctgtcaa ttgagacaac tcattccccg gagccctaca aaccatctga
    20401 ttcttcccat acccaccaat taatcccgga tcggttggtc cataaaccgt gatattgggt
    20461 ctatccagtg ccgccgttaa atggcttaac cccgtatcca ccgacactac aaatttagcc
    20521 ccggccagca cgcgggcaac gccttccaga ctcatcttcg gcaatacttc aacataatca
    20581 aatccttccg ccagtcgttt cgcccgttct tcctcatgcg gcgcgcccca cggaagttta
    20641 atccgtattc ctgaatcagc cagtaaacca atcaattctc gccagtgttc ttccggccag
    20701 tgtttatcat cacgggtcgt cgcatgaaga aatacggcat attcgccagc atctgtaggc
    20761 aggttcgtca gaaaatgctg tgcgatagca taatcgccct gggtttgcgg tttgctatag
    20821 cccaaacttt tggcaaacag ttcgcgggtg cgttctacgg cgtgctgctg ttttgcaata
    20881 tgatgcttac aattgtaaaa caggctggct aaaggttcgc gagcggtttg ccagtccaat
    20941 ccatgcttta cgccatgcgc cagacgtgtc accagtgccg cgctttttac cagcccctga
    21001 gcgtcgataa ctgcgtcata gttctctgct tgtagcgctt cacgaaacgc tttgcgttca
    21061 gcttttatgg gggccgagaa ccaggcttta cgccagcgac gtattgccac aggaataact
    21121 cgctcaacgg cagcgtgcca ggaaggaatc tgtgcgaacc cttcttccac cacccagtca
    21181 aacttaatcc ctgggattgc ctgctgggca tcagtgagtg cgggcaacgt atggagaaca
    21241 tcgcccatcg acgatgtttt aacgatcaaa acccgcatcc gtcaggcttc ctcttgtaac
    21301 aatagcgtgt tgagttcttc cagtacgcgc tggggagtaa tgtcgatcaa gctctggtga
    21361 taaccctccg cagcgtcacc tttacgcact ttgtgatagc cggtaatcag acggatcacg
    21421 cgcgctttat gggatagcgg cggtgtgaag tccgggctac ttggaccata cagggcaacc
    21481 agcggacgat tgagcgccgc cgcaacgtgc atcaggccag aatcgttagt gacaatggct
    21541 ttacaggctg caatcaggat aaccgcttga tcaagctgtg tttcccccgc caggttccga
    21601 caccatgcct gctgctcggt attcaaagcg gcaagaatct cattgcccgc ttcatgatct
    21661 ttcgcagagc caaacagaac cacctgataa ccttcatcaa tcagctgctt tgccagttcc
    21721 gcatagtggt agtgtggcca gcgttttgcc ggaccaaact ccgcacccgg gcaaaagcca
    21781 atcatcggac gttctgatga aagcgaaaat tgattacagg tatatgattt ttcaccttcg
    21841 ctcacctgca actgcggcca taacaatggc tgcggcagat cttgtgcggt acgcataatg
    21901 cctttgtcat aggccagcgc gacatagcgt tccaccatta gcggccaggc ttctttatcg
    21961 agcacgcgta catcgttgag taaaccgtag cgcatctcgc cgcgccagcc ggtgcgatga
    22021 ggaatacccg cgaagaaagg cactaatgca gatttgaagg agttgggtaa gacgtaggcg
    22081 cggtcgtagc gcttttcacg caggctatga cccagtttgc ggcgttcgcc gatttccagc
    22141 gctccgtgac cgagaggcat agggatagct tcgttaactt ccggcatccg cgataataat
    22201 ggacggcacc atgccggtgc catcacatcg attatcgcct ggggatagcg cgcctggagc
    22261 gtgcgataga gactttgcga catcatcatg tcgccaaccc aagacgggcc gatcaccagt
    22321 attttcatgc agagctctta tgcgtcgcga ttcagccagg ccatgtattc cgttacacct
    22381 tcagcaacgg ttttgaacgg tttgtcgtaa cccgccgcgc gcagatttgt cagatctgcc
    22441 tgagtgaacg cctggtagcg gcctttcagc ttatccggga acggaatgta ttcgatctgg
    22501 cctttcttgt gataagccag agtagcatct gctacggcct ggaaggattc cgcacgacca
    22561 gtaccgaggt tgaagatacc ggaaacgcca ttttccagga accacaggtt tacatctgcc
    22621 acgtcgccca catagacgaa gtcacgtttg aagttctcgc taccttcaaa cagcttcggt
    22681 gattcaccgt tgttaagctg agtgttgaga tggaaagcga cgctcgccat gctgccttta
    22741 tggccttcac gcggtccata aacgttgaaa tagcggaagc caacaatctg cgagttcgct
    22801 tcaggcagga tttggcgaac atattcatca aacaggaatt ttgagtaacc gtagacgttc
    22861 aacggttttt cgtactcgcg ggattcaata aagtcggagg tgcgtccgcc gtaggtggct
    22921 gcggaagaag cgtacaggaa cgggatttcg cgctccaggc agtagtgcag cagctccttg
    22981 gagtattgat agttgttatc catcatatac ttgccgtccc actcggtggt ggaagagcat
    23041 gcgccttcgt ggaaaatcgc ttcgacatcg ccgaactctt cgccagccat aatctggatc
    23101 aggaagtctt ccttatccat atagtccgcg atatccagat ccaccaggtt cacaaacttg
    23161 gtgccgtctt tcaggttgtc caccaccaga atatcggtga tgcctttatc attcagggcc
    23221 ttaacgatgt tgctgccgat aaagcccgcg ccgccggtaa cgatgatcat aactgtaacc
    23281 ttcgaattat ggagtcagag acaatctcag acacgaatac ttctatcata tcactatacg
    23341 gcgttggctt cagccattcg ccaaaagtga gattgatgga cgttatttat cctgaatttt
    23401 gcagaagtgt taacgcgtca tctcgtcgcg acgtataagt ttgggtaata tgtgctggaa
    23461 tttgccctgt ctggagaatc gcaatgcgtg gagaatttta tcagcagtta accaacgatc
    23521 tggaaaccgc acgggcggaa gggttgttta aagaagagcg cattattacg tctgcgcagc
    23581 aagcagatat cactgtggct gatggaagcc acgtcattaa cttttgtgcc aacaactatc
    23641 tcgggctggc gaatcatccg gatctgattg cggcagcaaa ggcgggaatg gattctcacg
    23701 gtttcggcat ggcttcggtg cgttttattt gcggcactca ggacagccat aaagagcttg
    23761 aacaaaaact ggcggcattc ctggggatgg aagatgcgat tctctactct tcctgctttg
    23821 atgctaacgg tggactgttt gaaacgctgc tgggtgcgga agacgctatt atctccgacg
    23881 cactgaacca cgcgtctatt attgatggtg tgcgtctgtg caaagctaaa cgctatcgct
    23941 atgccaacaa cgatatgcag gagctggaag cacgtctgaa agaagcgcgt gaagccggtg
    24001 cgcgtcatgt gctgattgcc accgatggtg tgttctcaat ggacggcgtg attgccaacc
    24061 tgaaaggcgt ttgcgatctg gcagataaat acgatgcctt ggtgatggtg gatgactccc
    24121 acgcggtcgg ttttgtcggt gaaaatggtc gtggttccca tgaatactgc gatgtgatgg
    24181 gccgggtcga tattatcacc ggtacgcttg gtaaagcgct gggcggggct tctggtggtt
    24241 ataccgcggc gcgcaaagag gtggttgagt ggctgcgcca gcgttctcgt ccgtacctgt
    24301 tctccaactc gctggcaccg gccattgttg ccgcgtccat caaagtactg gagatggtcg
    24361 aagcgggcag cgaactgcgt gaccgtctgt gggcgaacgc gcgtcagttc cgtgagcaaa
    24421 tgtcggcggc gggctttacc ctggcgggag ccgatcacgc cattattccg gtcatgcttg
    24481 gtgatgcggt agtggcgcag aaatttgccc gagagctgca aaaagagggc atttacgtca
    24541 ccggtttctt ctatccggtc gttccgaaag gtcaggcgcg tattcgtacc cagatgtctg
    24601 cggcgcatac ccctgagcaa attacgcgtg cagtagaagc attcacgcgt attggtaaac
    24661 aactgggcgt tatcgcctga ggatgtgaga tgaaagcgtt atccaaactg aaagcggaag
    24721 agggcatctg gatggccgac gtccctgtac cggaactcgg gcataacgat ctgctgatta
    24781 aaatccgtaa aacagccatc tgcgggactg acgttcacat ctataactgg gatgagtggt
    24841 cgcaaaaaac catcccggtg ccgatggtcg taggccatga atatgtcggt gaagtggtag
    24901 gtattggtca ggaagtgaaa ggcttcaaaa tcggcgatcg cgtttctggc gaaggccata
    24961 tcacctgcgg tcactgtcgc aactgtcgtg gcggtcgtac ccatctgtgc cgcaacacga
    25021 tcggcgtcgg cgttaaccgt ccgggctgct tcgccgaata tctggtgatc ccggcgttca
    25081 acgccttcaa aatcccagac aatatctccg acgacctggc ttccattttt gatcccttcg
    25141 gtaacgccgt gcataccgcg ctgtcgttcg atctggttgg cgaggatgtg ctggtctctg
    25201 gtgcaggtcc gataggtatt atggctgcgg cggtggcgaa acacgttggt gcacgcaatg
    25261 tggtgatcac tgatgttaac gaataccgcc ttgagctggc gcgcaaaatg ggtatcaccc
    25321 gtgcggttaa cgtcgcgaaa gaaaacctta atgatgtgat ggctgaactg ggcatgaccg
    25381 aaggctttga tgtcggtctg gaaatgtccg gtgcgccgcc agcgtttcgt accatgcttg
    25441 acaccatgaa ccacggcggc cgtattgcga tgctgggtat tccgccgtct gatatgtcta
    25501 tcgactggac caaagtgatc tttaaaggct tgttcattaa aggtatttac ggtcgtgaga
    25561 tgtttgaaac ctggtacaag atggcggcgc tgattcagtc tggcctcgat ctctcgccga
    25621 tcattaccca tcgtttctct atcgatgatt tccagaaggg ctttgacgct atgcgttcgg
    25681 gccagtccgg gaaagtaatt ctgagctggg attaacacga acaagggctg gtattccagc
    25741 ccttttatct ggggataatc tgttgaatat gtaaaatcct gtcagtgtaa taaagagttc
    25801 gtaattgcgc tgatctctta tatagctgtt ctcattatct ctctaccctg aagtgactct
    25861 ctcacctgta aaaataatat ctcacaggct taatagtttc ttaatacaaa gcctgtaaaa
    25921 cgtcaggata acttcagagg tcgtcggtaa tttatgatga acagcaccaa taaacttagt
    25981 gttattattc cgttatataa tgcgggcgat gatttccgca cttgtatgga atctttaatt
    26041 acgcaaacct ggactgctct ggaaatcatt attattaacg atggttcaac ggataattct
    26101 gttgaaatag caaagcatta cgcagaaaac tatccgcacg ttcgtttgtt gcatcaggcg
    26161 aatgctggcg catcggtagc gcgtaatcgt gggatcgagg tggcgacggg caaatatgtc
    26221 gcttttgtcg atgctgacga tgaggtctat cccaccatgt acgaaacgct gatgaccatg
    26281 gcgttagagg acgacctcga cgtggcgcag tgcaacgctg actggtcttt tcgtgagacg
    26341 ggagaaacct ggcaatccat ccccagcgat cgccttcgct caaccggcgt attaaccggc
    26401 ccggactggc tgcggatggg gctttcttcg cgccgttgga ctcacgttgt ctggatgggg
    26461 gtttatcgcc gtgatgttat tgttaaaaat aacattaaat ttattgccgg attacatcat
    26521 caggatattg tctggacaac agaattcatg tttaacgcgc tgcgtgcgcg atataccgag
    26581 caatcattat ataaatatta tctgcataat acgtcagtga gtcggttgca tagacaaggg
    26641 aataaaaacc ttaattatca acgtcactat attaagatta cccgcctgct ggagaaatta
    26701 aatcgaaatt atgccgacaa aattacgatt tatccggaat ttcatcagca aataacttac
    26761 gaagcattgc gtgtttgcca tgcggtgcgc aaagagccgg atattcttac ccgccaacgg
    26821 atgattgccg agatatttac ttccggtatg tataagcgcc tgattaccaa tgtgcgcagc
    26881 gtgaaggtcg gttaccaggc gttactgtgg tctttccgct tatggcaatg gcgcgacaaa
    26941 acgcggtcgc accatcgcat tacgcgtagc gcctttaatt tgcgctagcg ttaaacttcg
    27001 ccgcttcggg ctgtttgccc cagccttgcc actgatgctg gaagtaaacg atcagcgtgc
    27061 tctggctgat gctttcgctt aatacttcaa agaagcgatt agcataaaca ggttctatcg
    27121 gtttcttcgg tttgcacagc ttcacgccac ggaacggatt acgcggtgca tcaggcacag
    27181 cgtttttcgg cggtgtagaa gtatcaacct gcggttcatt cagcaagctg ctggctttca
    27241 ccagcgtaat gtctggcggc aggttataaa ccatctgttg tagcacgcgt accgttaaag
    27301 ggtgaggatg cccaatggca atggttgaac cgttgcgacg cgccagatca attgcgcgat
    27361 taaattgcac acggatgtcc gcttcgttct gcgaatcgtc gaggaacacc ttccgtttaa
    27421 taaccttcac gccagtacct tgcgcggcgc gcatcgcctg ggtattaccg atggttacgc
    27481 tgtcgaggaa gtaaagattg taacgctcca gtgcctgcat cactttctgc ataccaaaca
    27541 ggttagaggt catcttgcta cccatgtggt tgttgatccc tgcggcatag ggcacgttat
    27601 tgaccgcact acgaataatg cgctcaattt cgtcgctgct catctccggg cgcagcgtat
    27661 ttttctccag cggctgttta ctcagtggtg ccatcgggag atgaatcaac acttcgtgcc
    27721 cgctgttatg cgctttggtc gccatctctc tggcgtgcgg tgaatcgggt aatacagcga
    27781 cggagatagc ggagggcatc gccagcacct ggttttcgtt gtgcgggcga tacccaaaat
    27841 catcaatgac gatggcaagt ttgccagcaa gtacggggga ggagagcgcc aacagagcgg
    27901 caaatgcaag aacgttacga cgaaatggaa acaaaactta tcttcccaac cacggctgtg
    27961 gattgaccgc ctgaccctgg cggcgaattt cgaaatagag tgaaggccga ccctgaccgc
    28021 cactgctgcc caccagtgca attggctggc ccgcgcgaac ctgcgaacca acgctcacca
    28081 gtgcgctctg attatagccg taaagactca tgtcgccttt accgtgctca accaccacca
    28141 ccagaccgta accttgcagc cagtcagcca gaatcactcg accgtcggca attgctttaa
    28201 cttcagtacc ttcagaggca ccgataacca tccctttcca gcgtaattca ccctgtagct
    28261 gctcgccata gcgatgcagc gtcggcccgc gaaccggcca gaatgcctga ccacgcggcg
    28321 cacccagacc accagttcgg gacatcagcg atttttcgct ttcggtcggt ttgtaggtgg
    28381 tgcctttgcg cgtcgcttct ttctggcggt cgcgaaccgc ctgggcctca cgcgcttctc
    28441 gttcagcacg cgctttcgcc gcggcttccg cacgggcaat gctgttacgc agacgggatt
    28501 cgttggcacg cagttcactt agctgctgct gaccttgctg gatggaagat tccagccccg
    28561 ccagcgtctt tttacgctcg ttcagagcct gagtcagttt cgcctgttgg gcgcgctgtt
    28621 catataacag cgtttgttgc tcgctttgtt tctcttccag ttcagcacgc tgcatggcga
    28681 cttcttcacg cgtttgcttc aactgggcaa tggtttcttg tcgcgcctgg ttgagatagc
    28741 cgaaataagc ctgtaaacgc tgtccacact ggctttcttc accgctgaga attagctgaa
    28801 taccggtatg ctcgccctga cggaatgcgg catccagttg tgcggcgagg ctgcgctcct
    28861 gggcggcttt ttgctgctcc agtttggcaa tcgacgcgtt catctcatca atctgtttat
    28921 tgagttgatt gagcgtgttt tgcgtttcgc gcagcttacg ggtggcttca gagatcgctt
    28981 cttcctgctt tttcaattgt gcgagcaggc ttgcgcgttg ttgttgcttt tggcgtaccg
    29041 cgcgctcttt tgcggcgata tcggcctgaa tagatttgag ttggtcacgc acatccgcgt
    29101 gggcggaaaa ggcgcacaac aatacgccag cgctaagaac gctggcgtag atgatgggcc
    29161 tgattgcaaa cctgcgcggt ttcacggccc gtgtcatggt attaatcgcc tttcccctca
    29221 tggggaggga ttattccacg atgaacagcg gcttaccagt catctcttgc gggatttcca
    29281 tacccatcag cgacaacatg gtcggcgcga tgtcagaaag tttgccgcct gcaaccgctt
    29341 tcacgttctt atcaccaacg taaatcagcg gaactggcag gttggtgtgt gccgtgtgtg
    29401 cctgacccgt tgccggatcg cgcatctgtt cagcgttacc gtggtcagcg gtgatcagca
    29461 gttgtccacc cacggactca accgctttcg cgacttcttc cacgcagtga tccagtgctt
    29521 caaccgcttt aaccgccgct tccatcaccc cggtgtgacc taccatgtcg ccgttcggat
    29581 agttacagat aatggtgtcg tatttgccgc tcttgatggc cgcaaccagt ttttcggtca
    29641 gctctgcgga gctcatttcc ggttgcagat cgtaggtagc cactttcggt gagttgatca
    29701 gaatgcgatc ttcgcctttg aatgactctt ctacgccacc gttgaagaag aaagtaacgt
    29761 gggcatattt ttcggtttcg gaaatacgca actgagtttt gtcgtttttc gccatccact
    29821 cgccgaaggt gttaaccagg gaagcgggtg ggtaagcaac cgcagttttg atgtcagcgg
    29881 cgtattcggt cagcatcacg aaatcgacgt taaccacttt cttacgcgcg aagccgtcga
    29941 aatctgcgtt tacgaatgca cgagtgattt cgcgcgcgcg gtcagcacgg aagttcatga
    30001 aaatcagcgc atcgccgtct tccattgccg catctggctg accttctgca cgaataacgg
    30061 tcgctttcac gaactcgtcg ttttcgtcgc gagcataagc tgcctgcaaa ccagcaacgg
    30121 cagtatcggc ctggaactca ccctgcgcca gagtcagcag gtcataggct ttttcaacgc
    30181 gatcccaacg gttatcgcgg tccatcgcgt agtaacgacc aatgatggac gctacgcggc
    30241 ctttacccag cgcagcaaat ttctcttcaa attttttcag cgaggattca gcactgcgcg
    30301 gcggggtgtc gcgaccgtca aggaatgcgt gcaggtagat tttttctgcg ccgcgttcag
    30361 ctgccagttc taccatcgcc atgatgtgat cttcgtggct gtgtacgccg cctgcggaga
    30421 gcagacccat aatgtgtact gctttacctg cgtttttcgc tttatctacc gcgccagtca
    30481 gcactggatt agcaaagaaa gcgcgatctt tgatttcaac gtccagacga gtcaggtcct
    30541 gatacacgat gcggccggca cccaggttaa cgtggcctac ttcggagtta cccatctgac
    30601 ggtcaggcag accgacttcc agaccggaag cgtcgatcag ggtatgcgga cgattggccc
    30661 acagtgcatc cattaccggg gttttagcac taaaaatggc gttatcctgc tgttcttcac
    30721 gatagccata gccatccaga atcaccagta ccataggttt tttagaaacc gacattgcga
    30781 caacctcata ctcaagagtc aaaatttgcg taattttact acagctgaat cgataaaata
    30841 gcctctgaag atcaaaatat acgaccgctc gcggcaagcc gatgccagat ttgcgctttt
    30901 ttttcgtcgt gccccgcaaa attgcattcc agttaacgcg ctggctgtat ttgccgcacc
    30961 gcgcaggtat actcctttcc tggttttttt aatcactacg tcgggagttg ttacccccca
    31021 tgcaagaaat tatgcaattt gttggccgtc atcccatact gagtatcgcc tggatcgcgt
    31081 tactggtggc ggttcttgtg actacgttta agagcctgac ctcgaaagtg aaggtgatta
    31141 ctcgtggtga agctacgcgt ctgatcaaca aagaagacgc tgtggttgtg gatttacgtc
    31201 agcgtgatga cttccgtaaa ggccatatcg caggttctat caatctgttg ccgagcgaaa
    31261 tcaaagccaa caatgttggt gagcttgaga agcacaaaga caaaccggtt atcgtggtag
    31321 acggttctgg catgcagtgc caggagcctg caaacgcgct gacgaaagct ggttttgcgc
    31381 aagtattcgt gctgaaagag ggcgttgctg gctgggctgg cgaaaacttg cctctggtgc
    31441 gcggcaaata atttacctgt cgtctttcat gatgcaggcc gttagctgca attaggcccc
    31501 cagcgactta ttgttataag cttctgggga cactccattt tgcagccgcc ctgcaacatg
    31561 aaatttcagg gtaaaaaaac aggagctaaa ttatggccaa tgttgaaatc tataccaaag
    31621 aaacctgccc gtattgccat cgtgcaaaag cactgctgag cagcaagggc gtgagttttc
    31681 aggagttgcc gatcgatggc aatgccgcca agcgtgaaga gatgatcaaa cgcagcggtc
    31741 gcaccacggt tccccagatt tttattgacg cacagcacat tggcggctgt gatgacttgt
    31801 atgcattgga tgcacgtggt ggactggatc ccctgctgaa ataacgtgtg aacgttggca
    31861 ttacattgcg cagtatttaa ggacaacact taagggtttt ctacacatgt cagaacaaaa
    31921 caacactgaa atgactttcc agatccaacg tatttatacc aaggatatct ctttcgaagc
    31981 gccgaacgcg ccgcacgttt tccagaaaga ttggcaacca gaagttaaac ttgatctgga
    32041 tacggcatct tcccaactgg cagatgacgt atacgaagtg gtactgcgtg ttaccgtaac
    32101 ggccaccctg ggcgaagaaa ccgcgttcct gtgtgaagtt cagcagggcg gtattttctc
    32161 catcgcgggt atcgaaggca cccagatggc acattgcctg ggcgcatact gcccgaacat
    32221 tctgttcccg tatgctcgtg agtgcatcac cagcatggta tcccgcggta cattcccgca
    32281 actgaacctt gcgccggtta acttcgatgc gctgttcatg aactatttgc agcagcaggc
    32341 tggcgaaggt actgaagaac atcaggatgc ctgatgaacc aacgtaatgc ttcaatgact
    32401 gtgatcggtg ccggctcgta cggcaccgct cttgccatta ccctggcaag aaatggccac
    32461 gaggttgtcc tctggggcca tgaccctgaa catatcgcaa cgcttgaacg cgaccgctgt
    32521 aacgccgcgt ttctccccga tgtgcctttt cccgatacgc tccatcttga aagcgatctc
    32581 gccactgcgc tggctgccag ccgtaatatt ctcgtcgtcg tacccagcca tgtctttggt
    32641 gaagtgctgc gccagattaa accgctgatg cgtcctgatg cgcgtctggt gtgggcgacc
    32701 aaagggctgg aagcggaaac cggacgtctg ttacaggacg tggcgcgtga ggccttaggc
    32761 gatcaaattc cgctggcggt tatctctggc ccaacgtttg cgaaagaact ggcggcaggt
    32821 ttaccgacgg ctatttcgtt ggcctctacc gaccagacct ttgccgatga tctccaacaa
    32881 ttgctgcact gtggcaaaag tttccgcgtt tacagcaacc cggatttcat cggtgtgcag
    32941 cttggcggtg cggtgaaaaa cgtcattgcc attggcgcgg ggatgtccga cggtatcggt
    33001 tttggtgcga atgcgcgtac ggcactgatc acccgtgggc tggctgaaat gtcgcgtctt
    33061 ggcgcggcgc tgggtgccga gcctgccacc tttatgggca tggcggggct gggcgatctt
    33121 gtgcttacct gtaccgacaa ccagtcgcgt aaccgccgtt ttggcatgat gctcggtcag
    33181 ggcatggatg tacaaagcgc gcaggagaag attggtcagg tggtggaagg ctaccgcaat
    33241 acgaaagaag tccgcgaact ggcgcatcgc ttcggcgtag aaatgccaat aaccgaggaa
    33301 atttatcaag tattatattg cggaaaaaac gcgcgcgagg cagcattgac gttattaggt
    33361 cgtgcacgca aggacgagcg cagcagccac taaccccagg gaacctttgt taccgctatg
    33421 acccggcccg cgcagaacgg gccggtcatt atctcatcgt gtggagtaag taatgtcgtg
    33481 tgaagaactg gaaattgtct ggaacaatat taaagccgaa gccagaacgc tggcggactg
    33541 tgagccaatg ctggccagtt tttaccacgc gacgctactc aagcacgaaa accttggtag
    33601 tgcactgagc tacatgctgg cgaacaagct gtcatcgcca attatgcctg ctattgctat
    33661 ccgtgaagtg gtggaagaag cctacgccgc tgacccggaa atgatcgcct ctgcggcctg
    33721 tgatattcag gcggtgcgta cccgcgaccc ggcagtcgat aaatactcaa ccccgttgtt
    33781 atacctgaag ggttttcatg ccttgcaggc ctatcgcatc ggtcactggt tgtggaatca
    33841 ggggcgtcgc gcattggcaa tctttctgca aaaccaggtt tctgtgacgt tccaggtcga
    33901 tattcacccg gcagcaaaaa ttggccgtgg cattatgctc gaccacgcca cgggcatcgt
    33961 tgtgggtgaa acggcggtaa ttgaaaacga cgtatcgatt ctgcaatctg tgacgcttgg
    34021 cggtacgggt aaatctggtg gtgaccgtca cccgaaaatt cgtgaaggtg tgatgattgg
    34081 cgcgggcgcg aaaatcctcg gcaatattga agttggtcgc ggcgcgaaga ttggcgcagg
    34141 ttccgtggtg ctgcaaccgg tgccgccgca tacaaccgcc gctggcgttc cggctcgtat
    34201 tgtcggtaaa ccagacagcg ataagccatc aatggatatg gaccagcatt tcaacggtat
    34261 taaccataca tttgagtatg gggatgggat ctaatgtcct gtgatcgtgc cggatgcgat
    34321 gtaatcatct atccggcata cagtaactaa tctctcaaca acgctcccgg atatcccagc
    34381 tgccgccagg cttcatacac cactaccgac accgcattgg acagattcat gctgcggctg
    34441 tccggcacca tcggaatgcg aattttttgt tcagcgggca gggcatcaag aatgctcgct
    34501 ggcaggccgc gtgtttccgg gccgaacatc agataatcgc catcctgata gcttacggcg
    34561 ctgtgagcag gcgtaccttt cgtggtgagg gcaaacaggc gctggggatt ttctgcttcg
    34621 aggaacgcgc gatagtcatg atgacgcgta acggcggtaa actcgtgata gtccagcccc
    34681 gcgcggcgca ggcgcttatc gtcccaggca aatcccatcg gttcgatgat atgcagacga
    34741 aagccggtat tagcgcaaag acggatgatg ttgccagtat ttggcggaat ttctggttcg
    34801 taaagtacga tgtttagcat actgccccct tagtgcgggg gcaggatagc agaaaattgg
    34861 tgaaccgtgc ggccttttcc cctcaccccg gccctccccc tgtctcttga tcaggagatc
    34921 tgatcaagag atagctccct gagggagagg gtt
//
LOCUS       DAJTMK010000022        33960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTMK010000022 DAJTMK010000000
VERSION     DAJTMK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33960
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.022"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..202
                     /locus_tag="OO135_000917"
     CDS             <1..202
                     /locus_tag="OO135_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5396858.1"
                     /translation="FIRQGLGIALQPVLTLKSIAGELCSVPLEPTFYRQISLLAKEKP
                     VEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(244..1320)
                     /gene="glpQ"
                     /locus_tag="OO135_000918"
     CDS             complement(244..1320)
                     /gene="glpQ"
                     /locus_tag="OO135_000918"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5396859.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1325..2683)
                     /gene="glpT"
                     /locus_tag="OO135_000919"
     CDS             complement(1325..2683)
                     /gene="glpT"
                     /locus_tag="OO135_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5396860.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2956..4584
                     /gene="glpA"
                     /locus_tag="OO135_000920"
     CDS             2956..4584
                     /gene="glpA"
                     /locus_tag="OO135_000920"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5396861.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAANPALIGAVKVPDGTVDPFRLTAANMLD
                     TKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4574..5833
                     /gene="glpB"
                     /locus_tag="OO135_000921"
     CDS             4574..5833
                     /gene="glpB"
                     /locus_tag="OO135_000921"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5396862.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLDDDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLCPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5830..7020
                     /gene="glpC"
                     /locus_tag="OO135_000922"
     CDS             5830..7020
                     /gene="glpC"
                     /locus_tag="OO135_000922"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5396863.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7213..8115
                     /locus_tag="OO135_000923"
     CDS             7213..8115
                     /locus_tag="OO135_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5396864.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8156..8959)
                     /gene="yfaU"
                     /locus_tag="OO135_000924"
     CDS             complement(8156..8959)
                     /gene="yfaU"
                     /locus_tag="OO135_000924"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR5396865.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAVGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8977..10266)
                     /locus_tag="OO135_000925"
     CDS             complement(8977..10266)
                     /locus_tag="OO135_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5396866.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALEAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10323..11527)
                     /gene="rhmD"
                     /locus_tag="OO135_000926"
                     /pseudo
     CDS             complement(10323..11527)
                     /gene="rhmD"
                     /locus_tag="OO135_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11542..12324)
                     /locus_tag="OO135_000927"
     CDS             complement(11542..12324)
                     /locus_tag="OO135_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR5396867.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHLENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12544..13746)
                     /locus_tag="OO135_000928"
     CDS             complement(12544..13746)
                     /locus_tag="OO135_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5396868.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEENLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVCFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(13846..14388)
                     /locus_tag="OO135_000929"
     CDS             complement(13846..14388)
                     /locus_tag="OO135_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396869.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14667..15092
                     /gene="nudI"
                     /locus_tag="OO135_000930"
     CDS             14667..15092
                     /gene="nudI"
                     /locus_tag="OO135_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5396870.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPCQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15131..15733)
                     /locus_tag="OO135_000931"
     CDS             complement(15131..15733)
                     /locus_tag="OO135_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5396871.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            16041..17180
                     /gene="arnB"
                     /locus_tag="OO135_000932"
     CDS             16041..17180
                     /gene="arnB"
                     /locus_tag="OO135_000932"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5396872.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKCTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17184..18152
                     /gene="arnC"
                     /locus_tag="OO135_000933"
     CDS             17184..18152
                     /gene="arnC"
                     /locus_tag="OO135_000933"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5396873.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYRQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGDFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18152..18781
                     /locus_tag="OO135_000934"
     CDS             18152..18781
                     /locus_tag="OO135_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-L-Ara4N formyltransferase/UDP-GlcA
                     C-4'-decarboxylase"
                     /protein_id="HCR5396874.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLE"
     gene            18809..20134
                     /gene="arnA"
                     /locus_tag="OO135_000935"
     CDS             18809..20134
                     /gene="arnA"
                     /locus_tag="OO135_000935"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF005998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5396875.1"
                     /translation="MVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIA
                     PLLIACGDGALEIVTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLIL
                     GVNGFIGNHLTERLLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYH
                     VKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYG
                     MCSDKYFDEDHSNLIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMG
                     PRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIEN
                     AGNRCDGEIINIGNPENEARIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGK
                     GYQDVEHRKPSIRNAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20131..21021
                     /locus_tag="OO135_000936"
     CDS             20131..21021
                     /locus_tag="OO135_000936"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5396876.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCDYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIADSR"
     gene            21021..22673
                     /gene="arnT"
                     /locus_tag="OO135_000937"
     CDS             21021..22673
                     /gene="arnT"
                     /locus_tag="OO135_000937"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5396877.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYFLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEITQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLMLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22670..23005
                     /gene="arnE"
                     /locus_tag="OO135_000938"
     CDS             22670..23005
                     /gene="arnE"
                     /locus_tag="OO135_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5396878.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            23005..23391
                     /gene="arnF"
                     /locus_tag="OO135_000939"
     CDS             23005..23391
                     /gene="arnF"
                     /locus_tag="OO135_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5396879.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23385..23651)
                     /gene="pmrD"
                     /locus_tag="OO135_000940"
     CDS             complement(23385..23651)
                     /gene="pmrD"
                     /locus_tag="OO135_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5396880.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(23761..25116)
                     /gene="menE"
                     /locus_tag="OO135_000941"
     CDS             complement(23761..25116)
                     /gene="menE"
                     /locus_tag="OO135_000941"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5396881.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(25113..26075)
                     /gene="menC"
                     /locus_tag="OO135_000942"
     CDS             complement(25113..26075)
                     /gene="menC"
                     /locus_tag="OO135_000942"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5396882.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(26075..26932)
                     /gene="menB"
                     /locus_tag="OO135_000943"
     CDS             complement(26075..26932)
                     /gene="menB"
                     /locus_tag="OO135_000943"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5396883.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26947..27705)
                     /gene="menH"
                     /locus_tag="OO135_000944"
     CDS             complement(26947..27705)
                     /gene="menH"
                     /locus_tag="OO135_000944"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5396884.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMVACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27702..29372)
                     /gene="menD"
                     /locus_tag="OO135_000945"
     CDS             complement(27702..29372)
                     /gene="menD"
                     /locus_tag="OO135_000945"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5396885.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTTAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29461..30756)
                     /gene="menF"
                     /locus_tag="OO135_000946"
     CDS             complement(29461..30756)
                     /gene="menF"
                     /locus_tag="OO135_000946"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5396886.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIACHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30835..31140)
                     /gene="elaB"
                     /locus_tag="OO135_000947"
     CDS             complement(30835..31140)
                     /gene="elaB"
                     /locus_tag="OO135_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5396887.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31195..31656)
                     /locus_tag="OO135_000948"
     CDS             complement(31195..31656)
                     /locus_tag="OO135_000948"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5396888.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            31721..32638
                     /gene="rbn"
                     /locus_tag="OO135_000949"
     CDS             31721..32638
                     /gene="rbn"
                     /locus_tag="OO135_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5396889.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            32826..33960
                     /gene="elaD"
                     /locus_tag="OO135_000950"
                     /pseudo
     CDS             32826..33960
                     /gene="elaD"
                     /locus_tag="OO135_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8421 a         9060 c         8352 g         8127 t
ORIGIN      
        1 ttttattcga cagggattag gcatcgcctt acaaccagtg ttaacgctga aaagcattgc
       61 aggtgaattg tgttccgttc ctctcgaacc aactttctat cgacagattt cgttgctggc
      121 taaagaaaag ccggtagaag gcagtccact gtttttacta caaatgtgca tggaacaatt
      181 agtggcgatt ggaaaaattt gatatgagac aggatggcgc atgaacacca tcctgcagta
      241 acattactct ttattaagaa attttactgc cttatcaggg aaatcagtaa acagcccatt
      301 tacacccgct ttgttataca gagcatcata taactgattc acatcaggag tgtattcagg
      361 cagtttatct gaccgcacgg tataaggatg cactaccagt ttattctgct gagcatcttg
      421 caccatgcca gtgagtttga tattacccgg ctgcgatgtc tcctcaatca acatatggta
      481 atccggacca ataccatctg catattccgc cacctgtttc atggcacccg gcttaaacat
      541 ccagtcgtag ttgtaattaa cccagcttcc atccggctgt ttctgctgcg tttcattcca
      601 gtcggtatag gcaatcagct gtaccagatt gagctccatg cccattttgg gttccagctc
      661 attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt tatcgtcttt
      721 accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat ccttcccttc
      781 ctgatgatgg aaccacggcg ctttgatttc tggataaata ccgatatttt tcccggtaga
      841 gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgcaccc ggaagtcgga
      901 cttacccatt gggaaacgcc ccggataagt ctgcactttt ttaccgtttt caatatcgaa
      961 accttcggta aatttcaacg acttaatttc atccagcgtg aaatctatcg cgtagtaacg
     1021 accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac gatcgagata
     1081 atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct gttccagata
     1141 atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg gcaaatatcc
     1201 actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca ttgcactgct
     1261 tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagcg tcaatttcat
     1321 gccattagcc tccgttgcgt ttttgtagta attgttcatg gcgacgtttt tcgccaatca
     1381 tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc ataaagccgc
     1441 catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc accgaaccgc
     1501 ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt ttcggtgcca
     1561 gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag ccgataacaa
     1621 tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa acgatagtcg
     1681 cgatggtcac cagtgtcata aagaaaacgc cggttgcccc acggttgcca cggaagactt
     1741 tatccgacat ccagccgcac agcagagtgc ccggaatacc tgcatattca taaaggaagt
     1801 aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa gtcggtgacc
     1861 agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg atataccaca
     1921 gcagtttgtt cggcagtacg tactgcatga agatttgctt cgccgtcagc tcctgttccg
     1981 ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc ggcaagccac
     2041 aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga atggcgcaga
     2101 aagcaggcat atagagcgcc gcatgccagt cattgaacca ggccatcccc agcaggaaca
     2161 gcagcggcgg aataccacca ccaacgttgt gcgcacagtt ccacactgac acaatgccgc
     2221 cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc caccccatcc
     2281 cctggaacca accgcagagg aacaaaagta caaacatcac cgcaatgctc gacgtcgccc
     2341 atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct gcggggagga
     2401 aaacgcgcgg attcgagcga tctgataccg aacccattat gaatttcgaa aatccataag
     2461 cgatcgagat ccccgaaagc gcgaaaccta aatcaccgcg agagaatccc tgctcgacca
     2521 gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca tagccaaaga
     2581 atatccccaa gaaaatttgc cagcgcaatc gacgataagt cggatcgatc tccgcagcag
     2641 gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgatagc ctccgtggcc
     2701 cgtggtctta tttatgatta acagcctgat tcagtgagag aacctgccgt ttcttgagtt
     2761 gccgcgatgt taagaaaaca ttcataaatt aaatgtgaat tgccgcacac attattaaat
     2821 aagatttaca aaatgttcaa aatgacgcat gaaatcacgt ttcactttcg aattatgagc
     2881 gaacatgctc gaaatcaaac aattcatgtt tttactatgg ctaaatggta aaaaacgaac
     2941 ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat tatcattggc
     3001 ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct gcgcgtgatt
     3061 ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg cctgctgcac
     3121 agcggtgcgc gctatgcggt aaccgatgcg gaatcggccc gcgaatgcat tagtgaaaac
     3181 cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct gtttatcacc
     3241 ctgccggaag atgacctctc attccaggcc acttttattc gcgcctgcga agaagcaggg
     3301 atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc cgccaacccg
     3361 gcactgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg tctgaccgca
     3421 gcaaacatgc tggataccaa agaacacggt gccgttatcc ttaccgctca tgaagtcacg
     3481 gggctgattc gtgaaggcgc gacggtgtgc ggtgttcgtg tacgtaacca tctcaccggc
     3541 gaaactcagg cccttcatgc acctgtcgtg gttaatgccg ctgggatctg ggggcaacac
     3601 attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc gctgctgatc
     3661 atggatcacc gcattaacca gcatgtgatt aaccgctgcc gtaaaccttc cgacgccgat
     3721 attctggtgc ctggcgatac catttcgctg attggtacca cctctttacg tattgattac
     3781 aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct gcgtgaaggg
     3841 gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc tggcgtgcgc
     3901 ccgctggttg ccagcgatga cgatccgagc ggacgtaacg tcagccgtgg catcgtgctg
     3961 ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg tggcaaactg
     4021 atgacctatc ggctgatggc tgaatgggct accgacgcgg tatgccgcaa actgggcaac
     4081 acgcgcccct gtacgactgc cgatctggca ctgcctggtt cacaagaacc cgctgaagtt
     4141 accttgcgta aagtcatctc cttgcctgcc ccgctgcgcg gttctgcggt ttatcgtcat
     4201 ggcgatcgca cgcctgcctg gctgagcgaa ggccgtctgc accgtagcct ggtatgtgag
     4261 tgcgaagcgg taactgcggg tgaagtgcag tacgcggtag aaaatttaaa cgttaatagc
     4321 ctgctggatt tacgccgtcg tacccgtgtg gggatgggca cctgccaggg cgaactctgc
     4381 gcctgccgcg ctgccggact gctgcaacgg tttaacgtca cgacgtccgc gcaatctatc
     4441 gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat cgcctgggga
     4501 gacgcactgc gcgaaagcga atttacccgc tgggtttatc agggactgtg tggtctggag
     4561 aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg ccggattact
     4621 ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc gtggtcaaag
     4681 cgcgttgcat ttctcatccg gttcgctgga tttgctgagc catctgccag atggtcaacc
     4741 cgtggcagac attcacagtg gactggaatc tttgcgtcag caggcacctg cacatcctta
     4801 ctctcttctc gggccacaac gcgtgctcga tctcgcttgc caggcgcagg cattaatcgc
     4861 tgaaagcggt gcgcaattgc agggcagcgt agaactggct caccagagga ttacgccgct
     4921 cggcactctg cgctctacct ggctaagttc gccagaagtc cccgtctggc cgttgcccgc
     4981 gaagaaaata tgtgtggtcg ggataagcgg cctgatggat tttcaggcgc atcttgcggc
     5041 ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc tgccggaact
     5101 ggatgtcctg cgcaataacg ccaccgaatt tcgcgctgtg aatatcgccc gtttccttga
     5161 taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca atacctgcga
     5221 aatgatcctg atgcctgcct gcttcggcct ggacgatgac aaactgtggc gttggttgaa
     5281 tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg tgctgggcat
     5341 tcgtctgcaa aaccagttac agcgccagtt tgtgcgccag ggtggcgtgt ggatgccggg
     5401 cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct ggacccgcaa
     5461 tcacgccgat attccgctac gtccacattt cgcggttctc gccagcggca gtttctttag
     5521 tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc ttgatgtgct
     5581 acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc aaccgtggca
     5641 gcagttcggt gtaaccactg atgagacgct atgcccgtcg caggcagggc aaaccattga
     5701 gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc agggatgcgg
     5761 cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc aacgcgcagg
     5821 aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt ctgcaccacc
     5881 gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc cgggccggat
     5941 ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa atattgcatc
     6001 aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga tattatccag
     6061 cgcgcgcggg caaaatatga caccacgcgc ccgtcgctgc gtaattttgt gttgagtcat
     6121 accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac cgctacctcg
     6181 ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg tcgcacgcta
     6241 ccgaaatact ccttcggcac gttccgtcgc tggtatcgca gcatagcggc tcagcaagca
     6301 caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa ccatccgcag
     6361 ttaggtaaag atttaattaa agtgctcaac gcaatgggta ccggtgtaca actgctcagc
     6421 aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa agcacgcaaa
     6481 caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg cattccggtg
     6541 attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga agtgctgaat
     6601 gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct gtggcgcaag
     6661 ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt ttatcacact
     6721 ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt gcgtaaaatc
     6781 ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg tacttacggt
     6841 ttcaaaaaag agaactaccc cacctcacaa gccatcggcg caccactgtt ccgccagata
     6901 gaagaaagcg gcgcagatct ggtggtcacc gactgcgaaa cctgtaaatg gcagattgag
     6961 atgtccacaa gtcttcgctg cgaacatccg attacgctac tggcccaggc gctggcttaa
     7021 actcctttct gatgcccggt aagcatgtgg ttaccgggca tttttgcgta cacgattccg
     7081 tgcccaatgt atgcgttgca acgcagtgaa aattcctctg aaaacgtctc gcaaaggctg
     7141 aaactggcag atgtcaaagg cctgggataa ccgtaatgtc acgtcatcat aaatatcagg
     7201 tgacggacaa ccatgaccga atcaacaacc tcctccccgc atgatgcggt atttaaaacc
     7261 tttatgttca cacccgaaac cgcacgggat tttctcgaaa tacatttacc agaaccactg
     7321 cgcaagcttt gcaacctgca aaccttacgc ctggaaccca ctagttttat tgaaaaaagt
     7381 ttacgcgctt actactcgga tgttttgtgg tccgtggaaa ccagcgacgg tgacggctat
     7441 atctactgcg tgattgaaca tcaaagctct gcagaaaaga atatggcttt tcggctaatg
     7501 cgctatgcca ctgccgccat gcagcgtcac ctggataaag gctatgacag agttccgctg
     7561 gtggtgccat tgctgtttta tcatggcgaa acatcgccct acccgtactc acttaactgg
     7621 ctggatgagt ttgacgatcc gcaacttgcc cggcagttgt acaccgaagc ttttccgttg
     7681 gtggatatca ccatcgtacc tgacgatgag atcatgcaac atcggcgtat agctctgctg
     7741 gaactgattc aaaagcatat tcgcgaccgc gatttaatcg gcatggtcga caggatcacc
     7801 acgcttttgg ttagaggctt cactaatgac agccagctac aaacactgtt taattatctg
     7861 ctgcaatgcg gcgatacctc ccgtttcacc cgttttattg aggagattgc cgaacgttca
     7921 ccactacaaa aggagagatt aatgactatt gctgaacggc tacggcagga agggcatcaa
     7981 attggctggc aggaaggtat gcatgaacaa gccattaaaa ttgctttgcg catgctggaa
     8041 cagggcattg atcgtgacca ggtgctcgcg gccacccagc taagcgaagc cgatctggca
     8101 gcgaataacc actaattaac acaggcccac agccgatccc catgggcctt tgatatcaat
     8161 aactaccttt tatgcgtggc ccatttttgc ctgatttaaa catcgccagt cgttgatcca
     8221 gggcatcgct gtagagcatc gtgtcaacgc caacagcgac aaagttcgct ccccacgcca
     8281 ggcattgctg cgccatatca ggagccacag ccagaaaacc agccgcttta cccacagcac
     8341 ggatccgccg aatgctggtt tcaataattc gctgcacttc cgggtgcccg gcgttatccg
     8401 ggtagcccaa cgacgcagaa agatccgctg gtccaataaa cacgccatca atcccttcga
     8461 cgtcgaggat ctcgtccagg ttatccagtg ccgttttact ttccacctgc actaacagac
     8521 aaagcgaatc gttgacttgc gccatgtaat tctcaatgcg tccccagcgc gcagcccgtg
     8581 ccacactggc cccaacacca cgctcaccgt agggaggata gcgcgtggca gacgccacct
     8641 gacgagcctg atcggcagta tcgaccatcg ggatcaatag agtttgcgcg ccaatatcca
     8701 gaacttgttt aatcagcgat ttactgcctt ccaccggacg gatcacgggt tggctggcat
     8761 agggcgctac cgcctgtagc tggtgataaa gatcctgaat ggtgtttggc gcgtgctccc
     8821 cgtcaatcag caaccagtca taaccggaag tagcggcaat ttctgccata taagaagtag
     8881 tcgagcttaa ccacagacca atttgcactt cgcccttgcg taaacgttct ttaaagggat
     8941 tggttaataa tgcgttcatc gtgttcctta acctatttaa tgatgtgcca cgtcggtctg
     9001 cgcaacagta cgatttacac gcagagtgaa aataatcagc gaaccgatga ccgccaccgc
     9061 tgccagtgtc agtaatcctg ccgcatcgct ggcaaataac gtttctgctt tcacgcgcag
     9121 gatcggggca ataaacccgc ccactgcacc aaacaggttt acaaaaccaa tccccgcagc
     9181 cagcgccgta ccggaaagca gttgtgtcgg catcgtccag aacaccggct gcacggcaat
     9241 aaacccgata gctgcaacac acagcgctac gatcgccatt actggagaaa gcagcccgga
     9301 cagaccaata ccaatgcccg ccgccagtaa tgtcagcgct gcgacattac gccgttcgcc
     9361 ggttttatcg gaatagcgcg gaataagcca ggtcccaaac aaggccgcaa cccacggaat
     9421 ggcggtgacc accgacgctg taaagcccac ttttgttccc agcaatgccg caacctgggt
     9481 cggcaggaag aaaattaatc cgtaaaccgc cacctgaatg gtcaggtaga taatcgctag
     9541 ttgccagact cggccattac gcagcgcatc gctcagccga gaagtcactt tctgttgttc
     9601 ttcacttgcc agttgattga taagcaacgt tttttcttgt ttactcagaa aacgtgcctg
     9661 ctccggtgtg tcatcaagcc aaaagaatgt gaatacccca gcaccgactg ccaacaatcc
     9721 ttcaatcaca aacatccaga accagccggg atgccccata aatccatgca tctccaacag
     9781 cgcgccggaa agcggtgatc ccagtgttaa cgccagcggt gcgcccatat agaacagccc
     9841 cataatgctg gcgcgattac gctgcggaaa ccattgcgag gtgagataaa tcataccagg
     9901 gaaaaatccg gcctccgcag caccaagcag agtgcgaact atcagaaatt tcgcttcagt
     9961 atcggcccat gccatggctg ccgaaagaaa tccccacagc agtgttgtcg taccaatcca
    10021 ggttctggcc cccagtttgc gcatcaaaag attcgccgga acacccagaa acgcatatac
    10081 cacaaagaaa atgcctgctt ccagcgcata agcttcatta ctcaacccgg tatcaatctg
    10141 gtaggtctgt ttggcaaaac cgatattcga acggtcgaga aacgccagca catacagcgc
    10201 caacataaac ggaattaaac gcacacggtt tttcttcacc acggcgtcaa gcaaagcggt
    10261 gctcataaaa agctccttag aatatgtggg cgttaccgtt gtcacagcaa cgcccggagt
    10321 gattagtggc tgtaggggcg tttcaaattg cagtcacgat tgagttcgac gccaaaaccg
    10381 ggtttatcaa gcactgattt atgaatacga ccattcaccg gaaccggctc attgagcaga
    10441 atcgggtcaa actgcggacg catcgttgaa caatccgggc tggtcatcag gaattcgctg
    10501 aatggcgtat tggtgaaggt gatcaccgca tggtgagagt aaacagacga accgtgcggc
    10561 accaccagtt gccccctgga tttggcgatt gcggcaattt ccaccagcgt ggtaaaccac
    10621 cgcaccagcc aacatccggc tgcataatgt cgataccggt ttctgaaagc gtacgaaaag
    10681 attgcagtgt gccgtggtgc tcaccgctgg tgaccatcat tccgactggc gcgttgcgtt
    10741 tcagttcgcg ataaccttca tactgctgtg gcggcaggca ctcttcgatc catttcaggt
    10801 tatagggcgc gcaagcgtgg gctagtttgg tcgcatagtt cacgtcctga ctcatccagc
    10861 agtcgagcat taaccagaaa tcttcaccgc atttttcacg catatccgcg accatagcgg
    10921 catctttgcg gatccccgca tcgccatcat gtggccccca gtgcgtcggc attttgccac
    10981 cgataaagcc catctctttt gccagatccg gacgcgcacc tgtggcgtag aactgaatct
    11041 catcacgaac agcgccgcct aaaagtttat aaaccggaag cccgaccact ttgccgaaca
    11101 gatcccacag agccagatcg acacaggaaa tcgtattcat caccaagcca ccagagccgg
    11161 agtaatacag ggtggcattg agcatttgat cgtggatcag tttgatatca ctgacacatt
    11221 taccctcaat gaaacagtta aggtgttttt cgacgataaa acagcccatt tcaccggctg
    11281 tcgaaacagc gaatccggtc tggccgtttt ctgcttcgac ttcaacaacc aacgtgccaa
    11341 gaacgttaat gccaaatgac tggcgtgact gctcgtaatc gcggtattta ctcatcgggg
    11401 tggcaatatg atcgtctatc cagtgattcg ccccctggtc gttataatca ccgccgccag
    11461 cgcctttttc tgctgtcgca ccgccagtaa accaggcgcg aacctgttta atttttggta
    11521 gggtcatgat gttctccatt gttatgaggc ttgtaagtca aagggacttt tccatcccaa
    11581 cagacgtgaa atatccctgg cgcaggcaat ggccttgccc gccagataat cacggtattc
    11641 ttcattgatt tgtaagcggg taccgaccac cgagatcgca gcggtaagct cgttattggc
    11701 gttaaacacc ggcgcagcga cacaacggac atcggggtaa tcttcgccgt tgtcatagct
    11761 ccagccctga cggcgaatac gcgccagttc ttcgtgaagt tgctgtggat gagtaatcgt
    11821 tgtgggtgtc gcctgctccc agaccagccc ttcgataata ctttgctgta ccgctgcagg
    11881 ttgccaggca agcaggcatt tacctattcc ggagcgataa agcgaaaggc tttttccttc
    11941 atgggaacgc acgctgatag tggctgatga ctccactttt aaaatgtaat aagcgctgcc
    12001 gttatcaata atccccagat gacataacag cccggtggta tccatcagtt gcgtcaaacg
    12061 cggtcgagcc agttcccgga gatccatctt gcttaacgca tgaccagaaa gctccaccag
    12121 cctggtccac aaacaaaaat tttccaggtg atcaaggcta agaaaacgct gacgcctgag
    12181 ttcattaagc agcaaatagg ccgtgctttt agggattccc aatgtgtcaa tgatcgtcgc
    12241 agcactacag gggccaatgc gggcaatcaa attgagaata tcaatagccc gtgtcagggc
    12301 gggaactttg cttgattcca acatactgga ctccagtctt agatactgga atcagtctcg
    12361 ttgctcaggg ggagtaaaat tgtgaagcgc atcaaatccc gctcctcgtc cagcggactg
    12421 gagaaattac gcacaaattt gtgaatcagt gcacggattt tttcttattg agtgcaacag
    12481 gttggacaag ttttgccgga gggggaatgt gccggtgcag taaaccggca cagagtggcg
    12541 gaatcagaca gataaggtca tggactcaat aacctcaatc cagccatgct cactggcgat
    12601 atcctggccg tttaaccaac ggcgcagcat attcagtgcc atcattgcgc acacttcctg
    12661 acggatagcc aggctgtagc gcgtagtgct gaaacacaca cgcagagcga aagtgccgtc
    12721 tggtgtggct agcgcaaagt tgagatgttc gttctcgaaa cccgaaacag ccagtgccag
    12781 cccggcaaaa tggttggccc gccgttctgt aatccagtgc gcagtttgcg ccaggttttc
    12841 ttcctgtgaa ggaaccactt cacacgccag caatggagca cctgcgcgag aaagttgcaa
    12901 agccaataaa ccaccggtga attgctcgct caacgtcagg ctgaactggc ggttttgcaa
    12961 ttcgcgactg atctgcgcgg gcagtccttc ggtgccttcg aaaatcacgc tctgtccggc
    13021 aacacgttta acgtccagcc acagtttttc catcgcctgt tgctcgcttg ccggtccggt
    13081 gagtttcagt tcgataatcg gcattgaaga gcgatagccc attgttacgc ccggcggcag
    13141 ttgtagagtg tccaggcttt gtgccagatc gctttccgaa cgaccaaagg tagttaaacg
    13201 cagacaaacc ggcggctgtg gtaaagaaaa acgctcgcgc aggcgcggca ggatttcgtg
    13261 ctcgaccatc accttaaatt ctgacggtac gccgggagta aagaacatca ggcaacgatt
    13321 aagctgcacg gcaaaaccac aggcggtgcc taccgggtta ttgataaatt cagcactggc
    13381 aggcagctcc gcttgtttac ggttgctcgg tgccattact cgtccacgtt cgtggaaata
    13441 gcgttccatc tctttgagcc aggcttcatg cagcaccagg ccttcacctt ttgctgtcgc
    13501 agcggcgagt gcgcttaaat catcgctggt cggtcccagc ccgccgttaa cgatcagcac
    13561 atcggcgtgc tgactacgtt cgcgaagaat ggtgactaag tcatcaaggt tatcccccac
    13621 cgtattgcgg cgagataatg gcagcccctg atgaaagaaa aaatcggcca gccaggcagc
    13681 gttagtgtca acgatttgcc cgtgtaacac ttcatccccg gtggataaca tttccacttt
    13741 taacatggtt atctcctgct tttgttatcg aatcactata acgcaaaggg agataaggcg
    13801 tagtaagaaa ctgaatggcg cgacagaatg ccgcgccgga agggatcaga aactggcgtt
    13861 aaccccaaca tacgggccgt cagccacggc gttgtcgcgg ttaccatctt tacccgacaa
    13921 attcaggtag cgataacccg cctcaatact gactggacgc ataatggtgt aacgcgcgcc
    13981 agcattcgct tcctcataac tttgaatacc gctggagagt gaatccggag agtagtaata
    14041 ctcgccaaac aaacggaagc tgttgccaat tttccactgc aaaccacctc ctaccgctgc
    14101 ggcataacct tcatcgccgt aattcgggtt ggtgtaaacg ccttttccgc caacggtcgc
    14161 cattaacggc cctagaggca gattcaaccc cagccccacg ccagcgacgt caccgtcgtc
    14221 gtcgttatgt gtccagttac cgcttaacgc caggcccgtc gattcagtcc caaaaccgac
    14281 accaatattg gtgtattctt tacccgcctg accgctgatg ctcattgcat tgaccgatgc
    14341 agaaaccaac agcattccgg ccagacctgc tagcgcaatt tttttcatta tcccctttcc
    14401 cacaagcaat taatcaaaat tcccaaagct gcgggattgt actgcctggc acagaggatt
    14461 caatgagcga aaaaagggat ctagtcactt attgtaacta aatacttctt aaataattaa
    14521 acgccgaaaa aataaacaca ctttaccgat acacacaaaa ataataataa gtgcagttat
    14581 catcgcaaaa gagattactg tcactttcac atatttctga gttaccgtat tctcatggtt
    14641 tctttttatt tataaggaat acccctgtgc gacaacggac tattgtatgc cctttgattc
    14701 aaaatgatgg tgcttatctg ctgtgcaaaa tggccgacga tcgcggcgtt ttcccctgtc
    14761 aatgggctct ttcgggtggc ggcgtggagt atggcgaacg aattgaagag gcactacgcc
    14821 gcgaaattcg cgaagaactg ggagaacagc tgcttttgac agaaatcacg ccgtggacct
    14881 tcagcgatga tattcgcacc aagacgtatg cagatggtcg caaggaagag atttatatga
    14941 tttacctgat ttttgactgc gtttctgcca accgagaagt gaaaataaac gaagagtttc
    15001 aggactacgc gtgggtaaaa cctgaagatc tggtgcatta tgatttgaat gttgccaccc
    15061 gaaaaacgtt acgtttgaaa ggtcttcttt aacaatgatg acagccatca ctggctgtca
    15121 gattgaaaat ttagtggtta acgaattccc catccagata aactttgcct ttctccacat
    15181 gcatgactaa accatccaga taatcaggtt taaatgtcgc atcacgctta tttttagcaa
    15241 tatatgtcag gcaatgatta tgggtgaaaa taacgatatt tttatcaggc gctttgcttt
    15301 gtaagtcctt aattgcacta taaatctcat taccgcactg aagaagtcgt ttatctaccg
    15361 tcagcttttt acccgcggaa aaccaggtcg ccgactgaat ggtccggacg gtattactgg
    15421 aataaagatc gaaatcaggg atgtcagcgc taaaagcgtt gcccagttca cgggcatcct
    15481 gggtaccttt aaccgtaata cctgttttat ctgacaagca ttgattggtt gaacgatcgc
    15541 aacgttcagc atggcgaaac aaaacgacaa ccgggtgctg ctgtgccagt ctggccaatg
    15601 ttttattgtc gatacgtggc aaaccattgc tactccaggc ggcatgagta cccagtccgg
    15661 caattgcagc gagcgccagt aaaatgatga aatatttttt tgacttcaat gatgagcggc
    15721 aaaaagctaa cacgtaaact ccacctatag acaagcgcaa cctgacaatt accgtgaaat
    15781 tgagcgacat ttctggcgat aattagcagt tggtgtaata ttaaaaatcc tacgatgtcg
    15841 gcaaaatgcc tcaaaatttt gccaaatgca aagcctaaat aagaaaaaat ataaaaactt
    15901 caatatttac gtctaatatt agtttcttaa ggttaagtta atattctatc cttaaatttt
    15961 cgctccaaat ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt
    16021 caatggcgga aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg
    16081 tggaggaact cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga
    16141 agaatcaggc gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg
    16201 tcagttcagc caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg
    16261 atgaagtgat tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgttgg
    16321 gtgcaacgcc ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga
    16381 tcgagtcagc cattacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc
    16441 cagcagatat tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag
    16501 atgctgccca tgccgtcggt acgtattaca aagggcgaca tattggcgca aaatgtaccg
    16561 ctattttttc atttcatgcc attaaaaata ttacctgtgc ggaaggtggc ctgattgtaa
    16621 ctgataatga aaaccttgcc cgccagctac ggatgctgaa atttcacggc ctgggtgtcg
    16681 atgcctatga cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct
    16741 ataagtacaa tctgaccgat attaacgccg cgattgccct gacacagtta gcaaaattag
    16801 agcacctcaa cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc
    16861 tcccctttca gccattaagc cttcccgcct ggccgcacgt tcacgcctgg catctgttta
    16921 ttattcgtgt cgatgaacaa cgttgtggta tcagtcgcga tgcgttgatg gaagcgttaa
    16981 aagaaagagg cattggtacc gggttacatt tccgcgccgc tcacacacaa aaatattatc
    17041 gcgagcgttt tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt
    17101 ctttgccgct gttcccggat atgactaccg cagatgccga ccgtgtcatc acagcccttc
    17161 agcaactcgc aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg
    17221 ttattcccgt ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag
    17281 cctgtgaatc gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata
    17341 attccgcgca tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt
    17401 tgcttaaccg caattacagg caacattcag cgattatggc gggattcagt cacgttactg
    17461 gcgacttaat tattaccctt gatgccgatc tccagaatcc gccagaagaa atcccccgcc
    17521 tagtggcaaa agccgatgaa ggttacgacg tggtagggac tgtacgccag aaccgccagg
    17581 acagctggtt tcgtaaaaca gcttcgaaga tgattaaccg gcttattcag cgcaccaccg
    17641 gcaaagcgat gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg
    17701 cgatgttgca ctgccatgaa cgtagcacct ttatcccgat tctggcgaat atcttcgccc
    17761 gccgtgccat tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa tccaaataca
    17821 gttttatgcg cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc
    17881 tacgtatgct gagtctgctc ggcagcatta ttgcgattgg agattttagc attgcggtgc
    17941 tgctggtgat tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc
    18001 tatttgccgt gctgtttact tttattggcg ctcagtttat cggcatggga ttactcggtg
    18061 aatatatcgg caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag
    18121 ttatccgtcc atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga
    18181 tatgggatgc ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt
    18241 tacccatacc gataatcccg gtgaaaaagc cttttatggt tcggtggctc atctggcggc
    18301 ggaaagaggc attccggttt atgcgccgga taacgttaat catccgctgt gggtggaacg
    18361 cattgcccaa ctgtcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga
    18421 cgagattttg cagctcgctc ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa
    18481 atatcgtggt cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt
    18541 tacattgcac cgaatggtaa aacgtgccga tgctggggcc attgtggcgc aactgcgcgt
    18601 tgccattgcg ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg
    18661 ccagctactg gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca
    18721 gcgcgaaaac gaagccacct gttttggtcg cagaacgccg gatgacagtt tccttgaatg
    18781 acataaaccg gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg
    18841 tgccttcagc tatgttggca atcagaaatt caccgtctgg tcgtcgcgtg ttcatcctca
    18901 tgccagcaaa gcacagccgg ggagcgtgat ttctattgca ccgctgttga ttgcctgtgg
    18961 cgatggcgcg ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc
    19021 gcaattagcg cagacgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg
    19081 caccgcccga cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca
    19141 tctgacagaa cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag
    19201 cgatgcgata agccgttttc tgaatcatcc gcattttcac tttgttgaag gcgatatcag
    19261 tattcattcc gaatggattg agtatcatgt caaaaaatgt gatgtcgtct tgccgctggt
    19321 ggcgatagcc acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt
    19381 tgaagagaat ctgcgcatta tccgctactg cgtgaagtac cgtaagcgaa tcatcttccc
    19441 gtcaacttca gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa
    19501 tttaatcgtc ggcccggtga ataaaccacg ctggatttat tcggtatcaa aacaattact
    19561 tgatcgggtg atctgggcct atggcgaaaa agagggttta cagttcaccc tcttccgccc
    19621 gtttaactgg atgggaccac gactggataa ccttaatgcg gcacgaatcg gcagctcccg
    19681 cgctattacg caactcattc tcaatctggt agaaggttca ccgattaagc tgattgatgg
    19741 cggaaaacaa aaacgctgct ttactgatat tcgcgatggt atcgaggcgt tataccgcat
    19801 tatcgaaaat gcgggaaatc gctgcgacgg tgaaattatc aacattggca atcctgagaa
    19861 cgaagcgaga attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct
    19921 gcgccatcat ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg
    19981 caaaggatat caggacgtag agcatcgtaa accgagcatc cgcaatgccc accgctgcct
    20041 ggactgggag ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct
    20101 gcgcaccgtt gatcttacgg ataaaccatc atgaccaaag taggcttacg cattgatgtc
    20161 gatacctttc gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat
    20221 aatattcagg ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg
    20281 cgactggtga agccacagtt tttgtggaag atgctgcgct caaacgcggc atcgctttat
    20341 ggctgggata ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat
    20401 gccgatatca ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac
    20461 catgcctggc aagcccgtag cggtaactgg gatcggcaaa caatgatcga cgatattgca
    20521 cgcggtcttc gcactctgga agagattatc ggtcaaccgg taacctgttc tgccgctgcg
    20581 ggctggcgtg ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac
    20641 agcgattgtc gtggggccat gccgttccgt ccattgctcg aatcaggaaa ccctggcact
    20701 gcgcaaattc cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaaaca
    20761 gaagatttta acggttggtt actcaaccgc atcctgcgag ataaaggcac gccggtttat
    20821 accattcatg cagaagttga aggctgcgat tatcagcata attttgtgga tctcctcaaa
    20881 cgcgcagctc aggaaggcgt gacattttgc cctttaagcg aactgttatc agagacgttg
    20941 ccgctcggac aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa
    21001 caaattgcgg atagtcgctg atgaaatcgg tacgttacct tatcggcctc ttcgcgttta
    21061 ttgcctgcta ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc
    21121 gttatgcgga aatcagtcga gaaatgctgg catccggcga ctggattgtg ccccatctgt
    21181 tagggctacg ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat
    21241 ggctatttgg cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa
    21301 ctgccgcgct ggtgacctgg tttactctgc gcttatggcg cgataaacgt ctggctctac
    21361 tcgccacagt aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc
    21421 tcgatccgtt tattgcattc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc
    21481 aggcacagac gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca
    21541 tgggggtgat gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat
    21601 gggtagcgac gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca
    21661 gttgcgtact gacggttctc ccttggggac tggcgatagc gcagcgggag cctgacttct
    21721 ggcactattt tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata
    21781 gagctccgtt ctggtactac gtgccggtca tcattgccgg tagcctgccg tggctgggat
    21841 tactccccgg tgcactgtac acaggctgga aaaaccgcaa gcattccgca accgtctatt
    21901 ttttgagctg gacgataatg ccgctgctgt ttttctccgt cgctaaaggt aaattgccca
    21961 cctatattct ttcctgcttt gcatctctgg caatgctgat ggcgcattac gctttgctgg
    22021 cagcaaaaaa taatcctctg gcgctgcgga ttaatggctg gattaacatc gcttttggcg
    22081 tcactggcat tattgccaca tttgtggtct ccccgtgggg accaatgaac acgccggtgt
    22141 ggcaaacctt cgagagctat aaagtctttt gtgcctggtc gattttttcg ctatgggcat
    22201 ttttcggctg gtacacctta acaaacgtcg aaaagacctg gtctttcgcc gcgctttgcc
    22261 cgctggggct ggcgttgctg gtaggatttt caattcctga cagagtcatg gaaggaaaac
    22321 atccgcaatt ttttgtcgag ataacacaag aatcacttca accaagccgc tatattctta
    22381 ctgatagcgt cggtgttgcc gcaggtctgg catggagcct gcaacgcgat gacatcatca
    22441 tgtatcgcca gacaggtgag ttgaaatacg gccttaatta tccggatgcg aaagggagat
    22501 ttgtcagcgg tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta
    22561 cactcatgct ttcggttgac cgcgatgaag atatcaacag tctcgccatt ccgcccgcag
    22621 atgccatcga tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa tgatctggct
    22681 aacattagtc tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac
    22741 ctgctttgtg gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct
    22801 ggcttgtctt ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg
    22861 cattgcctac ccgatgttaa gtctgaattt tgtctgggtg acgctggctg cagtaaaact
    22921 gtggcacgaa ccggtatcgc cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat
    22981 tgtgatcctc gggagtacgg tgtaatgggc ctgatatggg gattattcag cgtcattatt
    23041 gcctcagttg cgcaattaag cctgggtttt gcggcaagtc atctgccgcc gatgacgcac
    23101 ttatgggatt ttatcgcggc tctgttagct tttggcctgg atgcccggat actgttactc
    23161 gggctgctgg gttatctgct ttccgttttt tgttggtata aaacgctaca taaacttgcg
    23221 ctaagcaaag cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg
    23281 gtacttcccg gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt
    23341 atgagcgggt tgatgctgat tttcctgccc acgacaaaac aacgttactg agttttccct
    23401 gccgctgtac actgccgttc ccactcatcg gggctataac tgcttgccga gaggactttc
    23461 accgtatgca gcttttcacg attaatacaa taaagcgcat tctgcaaagg agagagtaaa
    23521 tcccccactt tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca
    23581 gcatcgcaga gcataagtac atgtctgtta tcttgtttat tgcagcacga ttttttgacc
    23641 agccattcca ttgcattatc ctgtttgcta agagttttca catcaattta cagattaaca
    23701 gataaactct ttatcaacgg caatggaatg gcagaaatga gaagtgaaac ggagggttaa
    23761 ttattgctga cgttgcaccc actcttttag cgcctgacgt gaaattttaa taccgccgtt
    23821 tttcagctcc ggcggtaaag tcagccagcg caccggttgt tgaaaacgag ccagcttatc
    23881 tttcacccat tcgccaagat caacggtttg ctggtcatac tccaccaccg ctaccggccg
    23941 atgaccaaac tccttgtcgg caacgggaac gataaacacc tgcaaaaccg caggatgtgc
    24001 agcaataaca cgctcgactt cttccggctg aataccctca ccgccactga ataacagatt
    24061 gtctaaacgt ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc
    24121 gtaccagcct tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt aaccttctgc
    24181 catactggca gcccgcagcc acacttcatt attaacgatt ttcacttccc gacccggcag
    24241 cggcgaacca acatctgcca ggccgtcggc ttctttcgca cataccgtgg aggcaaactc
    24301 ggtcagacca tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc
    24361 gaccgggata gccgcgccgc caagtaacac cgctttcagg gaaacggaac tgcggttaac
    24421 cagcaaacgc cagagttgtg ttggcaccag tgaagcgtga gtacagcctg ccagcatttg
    24481 ctccaatggc tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc agcgccacat
    24541 aattccctga ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc
    24601 aaacggaatc agcgataaca caccttgcgc actggcaaga tgggcctgat aagtatggac
    24661 agcggctttc ggtaaaccgg tggagccgga ggtcaatgtc attgaacaaa gacgtgttgg
    24721 ttgccacaga gctgaatgtg tgccttcaac catctgaata tgcagtgaag ttaacgccgg
    24781 gaaagtattt tctccttcca gcaccagcgc atattgcagt gtaagatttg gcagtaattc
    24841 ctcaagcaac ggttgcggaa gctgtggatt tacgggcagc acccgcgccc cgcattgcag
    24901 taacgccagc caggcgagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct
    24961 gccctcaacc acaccctgcg ccgcaaagcc cgtagctaac tcatcgacgc gagcacaaag
    25021 ctcgcgccag ttgagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg
    25081 ccgccagtga cgccacggcc agtcagagaa gatcataaca accgctccag tgcatcaact
    25141 tccacgacag gcagcgtgct acccggccag cgacgtacct gctgcgcctg catcagatcc
    25201 agcgtgtcca gccctggaat ggtgtccggc gttaaccagg cggcaatccg cgccagttgc
    25261 gttaagccta agctcgactc aatagaagaa ctgatcaccg ccgtcagcct caacgcgtgc
    25321 gccgcctgta cctgctcgcg tactttttcc agactgcccg tgagcgtggg tttgataact
    25381 accgcgcgca cgccctcttc agccacaaag gcaaaatccg gctcgcgcag gctttcatcc
    25441 caggcaatgg caatgccggt ttcacgggca aacgctcgcg aatcatcgcg ggttttgcac
    25501 ggctcttcga gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc
    25561 tgacctttca gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt
    25621 gcctccagca acagattaac caccatgccg tcgcgcacgg cttcgtacaa tcccactttg
    25681 accttcgcca ctttctcgcc tggcatatca gcaagtttga ggatcagatc gtcaggatcg
    25741 ccattacaca gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc
    25801 gccaatgcac agcttacgcc aaaggcaacg gaaggcatct gcggtagctc gcaatcgcct
    25861 gccagccagt tatttaccca ggcaagcagc acgctttgcg cctcttccca ggtttcctgg
    25921 ctgaagcccg gcagtgggga gatctccccc cacccttcgc gctcgccctc acgcaggcaa
    25981 acgtacagcc cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg
    26041 gggatctgcc agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga
    26101 aatcaggctg acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt
    26161 agaacagcat ggtggcgttg cccgccaact cctgcagccc cgcctgcccg tcacagtcgg
    26221 cgttcagtgc agctttcagg cagcgcagcg ccatcggact gttttgcagc atttcgcggc
    26281 accagcgaac ggtctctttt tccagatccg ccagcggtac cacggtgttg acaaggccca
    26341 tatccagcgc ctgtttcgcg tcgtactgac ggcacaggaa ccagatttcc cgcgcttttt
    26401 tctgtccgac gatgcgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt
    26461 tcgggccagt ctgaccgaag atggcattat ctgccgcgat agtcaggtcg cacatcatgt
    26521 gcagaacgtg accgccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac
    26581 aggtacggat ctgacgctgg aagtccagca cgttcaggtg atgtacgccg gaatcatctt
    26641 tatagccgcc gtaatcacca cgcactttct ggtcaccgcc ggagcagaac gctttatcac
    26701 ctgcgccagt cagaatgatc acgccgacgt tgtcgtcata acgcgcatct gccagtgcct
    26761 ggatcatctc ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg
    26821 tgatttttgc gataccatcg gtggattttt cataacgaat gtcctcgaaa ccttcggagc
    26881 agtcgtgcca ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc
    26941 ctttagtcag aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg
    27001 atgcgcgtta tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag
    27061 ggcgcggaat ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct
    27121 aaggttagca cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag
    27181 ggttgcgcca ttattgttgc tgcgcagcgc caccagctcc cggcgttgat cgtcattgag
    27241 cgaggcaaaa acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgcg
    27301 aaaacgctgc gcccattggc gatcggaacg ctgacgttcc gcacgttgtt cagcattttg
    27361 cagccccgga tgcccgcctt cgacaataac cccacaaagc cccgccagcc cctggcaagc
    27421 caccatcatc gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt
    27481 gtaactaacc aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat
    27541 cgccgccgaa ccaccatgac ctgggagatc aacatacaac cgtgagtagt cggcaaacgc
    27601 ctcgcccact tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca
    27661 gggtaaacct ggttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc
    27721 gccagaagtt gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc
    27781 gtggtggttg gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc
    27841 ggacgatgat atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc
    27901 atcagataga aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg
    27961 ccgttgttgt tcaccacaat taataccagc ggcgcagaaa cctgacgcaa taacgccagc
    28021 gcgttgagat cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt
    28081 gcccgctgaa cgcctgcggt ggtcgaaagc agtccgtcga taccgctggc ccctcggttg
    28141 ctgtacaccg ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg
    28201 ctgttaccga caaacaattg cccctgttca ggcagatagt cgctgatgcg atgcgccagt
    28261 tgcgcttcgc caaacgcatc gcgacgggca ataaccgcct gcattgcctg ttccgccagg
    28321 cgcgggattt caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg
    28381 gcaatattgg caattaagcg gcgtccacga tggtgcgccg gatcaagccg cccttcgatg
    28441 tcatccacaa tccagtactc ttcgggttca cagcttgcct gccattgcag gagccgtttg
    28501 cccgtcaggc tgcttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct
    28561 ttggcattgc ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca
    28621 tcgccaatca gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc
    28681 gcactcatgc gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc
    28741 tgtttttcac tttccagact aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag
    28801 tcacccagac gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca
    28861 gcaaacgggc agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg
    28921 gtagaaacca gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc
    28981 gtggggtgag aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt
    29041 agctccggcg ggcgatcggc ggttaagaga atcagttttt ctccggttaa cccggcttca
    29101 atcagtgccg gatagagatt tgccaccgcc gtgccggagg tcacaatcac cgccaccggc
    29161 tgcttgctga ctttcgccag ccccagcgcc agatgcccca acccacgctc atcgaaatgg
    29221 gtgtggtgaa tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag
    29281 cctggggcga tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc
    29341 gcccagcgtc ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct
    29401 aagtataagg agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta
    29461 ctattccatt tgtaataaag tacgcaaccc tgccgctttg ttgtcgattt cctgccactc
    29521 ttgctcgggg tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc
    29581 gctaattttt gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc
    29641 cgcagaaccg gcgtaccatt ctcgggtgaa cggttcgtga caggcgataa actgtcgcgc
    29701 cagatcgcgc ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac
    29761 atcatccgct ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag
    29821 acgcagtacc tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca
    29881 gatatcttcc accaccagca tgttctcgcg ctggttttta tcatccgcca tcagccactc
    29941 ttctaactgc tgcgcctgct tatcatcagg atgatttgct actgttcccg ccagcgcttc
    30001 agtacgcagc gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc
    30061 attttcgcca tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc
    30121 agccatcatc gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag
    30181 caccactttg tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt
    30241 ccagcccgtt ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa
    30301 gggcttgata ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag
    30361 ggagctttcg ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc
    30421 caggcggggt aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg
    30481 taagtcggcg tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa
    30541 acgggtaatc gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa
    30601 ttgcgggtac gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg
    30661 gaaaggaata tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg
    30721 cagtagattt tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc
    30781 tcacattata cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg
    30841 gcgtgccagc aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca
    30901 aggtttttcg tggacgtagt catcagcacg ataaacagcc tgcttcgccc gatagtaata
    30961 actgtctgaa acctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc
    31021 tttcagctca acatatttct gatcagcggg atcgccagag gagcggagca cctcttccag
    31081 tgtttcacta agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat
    31141 tctcgttctc catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc
    31201 ctgaattacc tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt
    31261 cacagggata aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta
    31321 aacaggctta tcaggccagt gatgcgtgca ggtgtcgagg gttttactca tcagttgctg
    31381 ccccactttt tcgccacgca acgcttcact gacaatcacc cgaccaataa cgaccggctc
    31441 aagatcatca tcacttttca gaatcctcgc atatgccacc agttcatcat ttttccaacc
    31501 gagaatatgg cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt
    31561 ctgctcgaca acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga
    31621 taattcagag tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc
    31681 cttatactag ccagaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg
    31741 tacttcagcc ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca
    31801 acatccgacc cagagcggac tttggttgtt tgactgcggt gaaggcaccc agcatcagct
    31861 actgcatacc gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg
    31921 cgatcatctt tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca
    31981 acccttaacg atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat
    32041 tagcggctca tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct
    32101 cgatgatggc ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg
    32161 ctatcgtatt gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc
    32221 tggcgtgccg cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga
    32281 agatggaagg cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct
    32341 cgctattttc ggcgataccg gcccctgcga tgccgcactt gacctggcta aaggtgtcga
    32401 tgtcatggtg cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg
    32461 ccatagctct acacgccagg ctgcgacact agcccgtgag gctggagtcg gtaagctaat
    32521 cattacccac gtcagctcgc gctatgatga caaaggttgt cagcacctgt tacgtgaatg
    32581 caggtcaatt ttcccggcga ctgaactagc gaatgatttc accgtgttta acgtttaact
    32641 ctgcttatta ttaaaaacag ggcgaaactt gccctgttat cgcaacccgc gctacatata
    32701 tccacccaca cgggcatatt gagcgatttt tgatatttat tcatctcctt acgcactata
    32761 ttcagagcaa taagttgcct ctttttatct tcattcagca caacctttgc aactaaaagg
    32821 agcccatgat ggttacagtt gtcagcaatt attgtcaatt atctcaaacc caactcagtc
    32881 agacatttgc agaaaaattt actgtgaccg aggaattact gcagtcttta aaaaaaacag
    32941 cgttatccgg agatgaagaa agtattgagt tactgcataa tattgcgtta ggttatgatg
    33001 aatttggaaa aaaagctgaa gatattcttt accatattgt tagaaaccca acaaatgaga
    33061 ccctatcgat tatccgactt attaaaaatg cctgtttaaa attatataat ttggcacata
    33121 ccgcaaccaa cttccacctt aaaccaactg gtccggataa ttcagatgtt ctcctgttca
    33181 aaaagctatt ctccccttcg aaattaatga caattatcgg tgatgaaatt cctcttatat
    33241 ctgaaaaaca gtcgctttca aaggtgcttt taaatgatga gaataatgaa ctgagtgatg
    33301 gtacaaactt ctgggataaa aatcgtcaat taaccacaga tgaaatagct tgctatcttc
    33361 agaagatcgc cgctaatgca aaaaatactc aagtcaatta tcttactggt ctctacgtcc
    33421 cctactccac cagaactcac ctggaagacg ctctcaatga aaatattaag agcgatccat
    33481 catggccgaa tgaagtccag ttattcccca taaatactgg cggacactgg atattagttt
    33541 cgctacagaa aatagtaaat aaaaaaataa taaactacaa ataaaatgtg tcatattcaa
    33601 ctcattgcgt gcactaggcc atgataaaga aaattcactt aagcgtgtca ttaatagttt
    33661 taattctgaa ctcatgggag aaatgtcgaa taataatata aaagttcatt taaatgaacc
    33721 agagataata tttttacatg ccgatcttca gcaatactta agccaaagtt gcggtgcgtt
    33781 tgtgtgcatg gcagcccaag aagtgattga acaaagggaa agcaattctg acagcgcccc
    33841 ctatacgtta ttaaaaaact atgctgacag atttaaaaaa tattcagcag aagagcagta
    33901 caaaattgat tttcaacatc gactggtaaa caggaattgt tatttagata aatatggcga
//
LOCUS       DAJTMK010000023        33829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTMK010000023 DAJTMK010000000
VERSION     DAJTMK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33829
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.023"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..368
                     /locus_tag="OO135_000951"
     CDS             <1..368
                     /locus_tag="OO135_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5396890.1"
                     /translation="RYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINPTI
                     IYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIRNH
                     RYTATMLQHIAEDRDGTNH"
     gene            complement(365..1842)
                     /gene="scpC"
                     /locus_tag="OO135_000952"
                     /pseudo
     CDS             complement(365..1842)
                     /gene="scpC"
                     /locus_tag="OO135_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(1866..2651)
                     /gene="scpB"
                     /locus_tag="OO135_000953"
     CDS             complement(1866..2651)
                     /gene="scpB"
                     /locus_tag="OO135_000953"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5396891.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(2662..3657)
                     /gene="meaB"
                     /locus_tag="OO135_000954"
     CDS             complement(2662..3657)
                     /gene="meaB"
                     /locus_tag="OO135_000954"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5396892.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(3650..5794)
                     /gene="scpA"
                     /locus_tag="OO135_000955"
                     /pseudo
     CDS             complement(3650..5794)
                     /gene="scpA"
                     /locus_tag="OO135_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(5998..6891)
                     /gene="argP"
                     /locus_tag="OO135_000956"
     CDS             complement(5998..6891)
                     /gene="argP"
                     /locus_tag="OO135_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5396893.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSLIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7274..7933
                     /gene="rpiA"
                     /locus_tag="OO135_000957"
     CDS             7274..7933
                     /gene="rpiA"
                     /locus_tag="OO135_000957"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5396894.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8188..9420
                     /gene="serA"
                     /locus_tag="OO135_000958"
     CDS             8188..9420
                     /gene="serA"
                     /locus_tag="OO135_000958"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5396895.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            9649..9708
                     /gene="ibsC"
                     /locus_tag="OO135_000959"
     CDS             9649..9708
                     /gene="ibsC"
                     /locus_tag="OO135_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5396896.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10041..10100
                     /locus_tag="OO135_000960"
     CDS             10041..10100
                     /locus_tag="OO135_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5396897.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10201..10803)
                     /locus_tag="OO135_000961"
     CDS             complement(10201..10803)
                     /locus_tag="OO135_000961"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5396898.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(10824..11007)
                     /gene="ssrS"
                     /locus_tag="OO135_000962"
     ncRNA           complement(10824..11007)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO135_000962"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11049..11378)
                     /gene="zapA"
                     /locus_tag="OO135_000963"
     CDS             complement(11049..11378)
                     /gene="zapA"
                     /locus_tag="OO135_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5396899.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            11546..12124
                     /gene="ygfB"
                     /locus_tag="OO135_000964"
     CDS             11546..12124
                     /gene="ygfB"
                     /locus_tag="OO135_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5396900.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12150..13475
                     /gene="pepP"
                     /locus_tag="OO135_000965"
     CDS             12150..13475
                     /gene="pepP"
                     /locus_tag="OO135_000965"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5396901.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            13472..14650
                     /gene="ubiH"
                     /locus_tag="OO135_000966"
                     /gene_synonym="visB"
     CDS             13472..14650
                     /gene="ubiH"
                     /locus_tag="OO135_000966"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5396902.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            14673..15875
                     /gene="ubiI"
                     /locus_tag="OO135_000967"
                     /gene_synonym="visC"
     CDS             14673..15875
                     /gene="ubiI"
                     /locus_tag="OO135_000967"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5396903.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(15949..16032)
                     /locus_tag="OO135_000968"
     CDS             complement(15949..16032)
                     /locus_tag="OO135_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396904.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16322..17416
                     /gene="gcvT"
                     /locus_tag="OO135_000969"
     CDS             16322..17416
                     /gene="gcvT"
                     /locus_tag="OO135_000969"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5396905.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17440..17829
                     /gene="gcvH"
                     /locus_tag="OO135_000970"
     CDS             17440..17829
                     /gene="gcvH"
                     /locus_tag="OO135_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5396906.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            17948..20821
                     /gene="gcvP"
                     /locus_tag="OO135_000971"
     CDS             17948..20821
                     /gene="gcvP"
                     /locus_tag="OO135_000971"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5396907.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21087..21830
                     /locus_tag="OO135_000972"
     CDS             21087..21830
                     /locus_tag="OO135_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5396908.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGELGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(21887..23320)
                     /gene="bglA"
                     /locus_tag="OO135_000973"
     CDS             complement(21887..23320)
                     /gene="bglA"
                     /locus_tag="OO135_000973"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5396909.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23365..23676
                     /locus_tag="OO135_000974"
     CDS             23365..23676
                     /locus_tag="OO135_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5396910.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            23840..24499
                     /locus_tag="OO135_000975"
     CDS             23840..24499
                     /locus_tag="OO135_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5396911.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(24576..25556)
                     /gene="ygfZ"
                     /locus_tag="OO135_000976"
     CDS             complement(24576..25556)
                     /gene="ygfZ"
                     /locus_tag="OO135_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5396912.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            25799..26065
                     /gene="sdhE"
                     /locus_tag="OO135_000977"
     CDS             25799..26065
                     /gene="sdhE"
                     /locus_tag="OO135_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5396913.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26046..26453
                     /locus_tag="OO135_000978"
     CDS             26046..26453
                     /locus_tag="OO135_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR5396914.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(26493..27014)
                     /gene="fldB"
                     /locus_tag="OO135_000979"
     CDS             complement(26493..27014)
                     /gene="fldB"
                     /locus_tag="OO135_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5396915.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27126..28022
                     /gene="xerD"
                     /locus_tag="OO135_000980"
     CDS             27126..28022
                     /gene="xerD"
                     /locus_tag="OO135_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5396916.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28047..28757
                     /gene="dsbC"
                     /locus_tag="OO135_000981"
     CDS             28047..28757
                     /gene="dsbC"
                     /locus_tag="OO135_000981"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5396917.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            28763..30496
                     /gene="recJ"
                     /locus_tag="OO135_000982"
     CDS             28763..30496
                     /gene="recJ"
                     /locus_tag="OO135_000982"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5396918.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            30587..31685
                     /gene="prfB"
                     /locus_tag="OO135_000983"
     CDS             join(30587..30661,30663..31685)
                     /gene="prfB"
                     /locus_tag="OO135_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5396919.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            31695..33212
                     /gene="lysS"
                     /locus_tag="OO135_000984"
     CDS             31695..33212
                     /gene="lysS"
                     /locus_tag="OO135_000984"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5396920.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33255..33803)
                     /gene="idi"
                     /locus_tag="OO135_000985"
     CDS             complement(33255..33803)
                     /gene="idi"
                     /locus_tag="OO135_000985"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5396921.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
BASE COUNT         7969 a         8579 c         9153 g         8128 t
ORIGIN      
        1 gccgttatga agagcgtcaa gagcaagagc tgcttcagga gctgccctgg ctacaaatgc
       61 gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc gaatatgcga ccttgtggga
      121 tcaatcccac cattatttat cgtccctaca gttttatggc caaaatcagc gccgtggagc
      181 gcggacattt tctgacggtg atcccgcatt ttgcctggcg actggtgaat ccggcaacgc
      241 tgaaatattt tgatgctccg cacaggccga tgtatatgca ggaatatctt tattcaatca
      301 gaaatcatcg ttataccgcc acgatgcttc agcatattgc tgaagatcgt gacgggacaa
      361 accattaacc ctgcatcgag ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc
      421 taagatcgtg gtgaatatgt ccgccgggcg cattttccag atagcgatgc agataatccc
      481 gatacatagg atgtgcacaa ttatcaatga tagtgcgggc gcgttgaagc ggggaaagac
      541 cgcgcagatc ggcgatccct tgttcggtga tgatcacttt gacgctgtgt tcgctgtgat
      601 caacatggct gcacattggc acgacggttg agatcttgcc ttctttagca atcgacggag
      661 ccataaagat cgacagatac gcgttgcgtt caaaatcacc gctgccaccg atgccgttca
      721 tcagatcgac cccggctacg tgcgttgaat ttgcatgccc gtaaatatca aactccaggc
      781 cgacgttcag agcgatgacg cccagacgac ggatgatttc cgggttattg gaaatctcct
      841 gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt gtcgtaaatc ttgcgcaggg
      901 aatcggccga gattgtcagg ctggaggcgc tggccccgct gattttgtcg gtttccagta
      961 aatgcaccac cgattcctgt agcacttccg aatacatcat gaacggagga atttccgggt
     1021 tttcccccag acgcgccatt accgcattat tgatattgcc cacgccactt tgcagcggca
     1081 gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa taagaacgtg accacgttat
     1141 cggcaatctg ctggcacatg ggattttgct tatccagcat attaccggcg tcgggcaagt
     1201 tggtttccac gacggcgaca atctttttcg gatcgatttg cacatagcgg gtaccgacgc
     1261 gatccattgc atggaagatc gacacgctat tgcgccgcgg tggcgcgcca ggaatcacaa
     1321 tatctgccag tactgcaacg cgcggatcgt gatagtgatt aagttcaatg atcactttct
     1381 tcgctcgcag cagccaggtc ggcgcattac cgatcccgct ggttaaccag actcgaccat
     1441 ccggtgccag tgccgatgct tcaatgacgg caacatcaat gtcgccgaag aaaccgtaat
     1501 tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa actcaccgcg ccctgattga
     1561 tctttttacg taaaccggac gatgttgata tggcgcacgc caggaaacag catcggcgtc
     1621 agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc gtcagcaggc gaatttgata
     1681 cggctttttg gcctcatgct gttcgttagc tctgcgggca atcgcggtgg gtagggcttt
     1741 cggcgaaccc gccggggtaa agctgctaaa tgccaccatg tcgttatgct ggataatttc
     1801 tgccgcttca tcggcggtca tccttgtcca ctgagtttcc atttttactc cttcgttcgc
     1861 agggattaat gaccaacgaa attaggttta cgtttttcga ggaaagcgtt cattccttcc
     1921 tggtaatctt cgctgtcata aaccgcgcgt cgcatcccct gaatacgttc aaattcatcg
     1981 gagttcatgg tgtgcgcttc gcccagtaca cgcagctctt ctttgataac agcaatggct
     2041 aacggcgctt tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc cagttcttcc
     2101 acttccacaa catggttgag gatgccgaca gccagcgcgc gctgggcggt gattggcgaa
     2161 gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg
     2221 ccgaccaggt tatacgggac gccgaggttt acaggcgtca ttgagaaggt tgaggtactg
     2281 gcggcgatga tcagatcgga actcatgatc atttcaaatg cgccacccca aacactacct
     2341 tccaccatcg aaatgatcgg tttcgggaat ttttggatca tgcgggtgat ttgacgcaat
     2401 ggatcatcat aggagagcgg atcgcgaccg ccagacggca gttcgtgaat atcgtgacct
     2461 gcggagaaga ctttggatcc actcggcgcg cgcaaaatga tacagcgaat ttccggccgg
     2521 ttgagatcgc ttaacgcctg cataagatca tcaataaaga ctttacttaa ggcattaagt
     2581 tttcggccat agttaaactc aatgaccgcc actttgttga tagtggcaac gttcacatac
     2641 tgataagaca taaaaattcc tttaatcaaa atattgcgtc tggataaatt cactgagctg
     2701 ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg tctggcggta
     2761 atagcgatcg aaatcttcat tcgcgaacag gtgattcagt acttcttctt cggtctgctt
     2821 acgcagccat tccaccgatt gttgttgccg cacttgttgt aaacgaccac tggcagttag
     2881 cgcggttttg aactcgatga tggcgtgcca gatctcatcg attccacgtt tttccagtgc
     2941 gctacaagtc agaacccgtg gctgccattc gtcgtattta cgtcgcagaa tatgcagggc
     3001 actctcgtac atatgccggg caatggcgac attggtatgg ttatcgccat cgtctttgtt
     3061 gataacgatc agatcagcca cttccatcag ccctttttta atgccctgca gatcatcgcc
     3121 gccaccggca atttgcaacg agataaaaca gtccaccatg cgggcgactt ctgtttccga
     3181 ctgcccgacg ccaaccgttt cgacaatcac tacgtcataa cccgccgctt cgcataacag
     3241 cattaattcc cgcgctcgct gactggcacc gcccagatga ccggaggatg gtaccgggcg
     3301 aataaacgcc gcttcggcac gcgccaggtc attcatgcgg attttatccc cgagaatgct
     3361 accgccagtg accgggctgc tgggatcgac cgcaataacc gcgaccttta atccctctcg
     3421 aatcaacaac atgccaaagg cctcaagaaa ggtacttttc cccgcgctgg gggtgccggt
     3481 aacgcccagt cgcagggtgt taccgcagta cggcataatg gcatcaagca gctgcgtact
     3541 tagtgcctga tgacgcgggt gacggctttc caccagcgtc atggcctggg cgagtgtggc
     3601 acgctcaccc tgacgtaagc ggcgaatact ttctgccagc gtggcttcat taatcatgat
     3661 gctggcttat cagattcagt acgtcgcgca cactgtcgag cataggtgta cctggaccat
     3721 aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc ctgcggtgga atgacgccac
     3781 cggcgactac gcagatatct tcgcgtcccc attttttcag cgcctcgacc agttccggga
     3841 tcagcgtttt atgaccggca gccagtgagg atgggcccac tacgtgaacg tcattttcta
     3901 ctgccaggcg ggcgatctct tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac
     3961 cgagatcgga ataggcgctg gcgatcactt tcgagccgcg atcgtgccca tcctggccca
     4021 ttttggcgat cagaatgcgc gggcgacgac cattgtcggc aaggaactgc tccgtttgcg
     4081 caacaatggc atcgaactcg gaggccgatt tctcagattg atgatagctt tgcgcaatca
     4141 cgccggtaac acactggctt ggcaccagat aacggtcgaa agcggcttcc agcgcatcgg
     4201 aaatttcacc cagggtggcg cgaacgcgag cggcattaac agcggcagcc agcaggtttt
     4261 cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc tgttacggcg gcatcatcac
     4321 gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga
     4381 tctcaagtac atcggtttcg tcttcgtgat ccagtttgta cttgttgaca ccaacgatga
     4441 cacgtttgcc ctggtcgata agcgactgtt cgcgcgctga ggcctattcg atcattcgtt
     4501 ttggcagacc tgcttcgatc gctttcgcca taccaccggc ttcgtcgatc tgttggataa
     4561 tagctctggc ttgtttgacg atttgatcgg tcagcgactc aatgtaatag gatccggcca
     4621 gtggatcgac ggtgcggcag agttctgatt cttcctggat gatgatctgg gtgttgcggg
     4681 caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca
     4741 gtgactgagt accgcccagc gtcgcagcca gtgcttcaat ggtggtgcgg ataacgttgt
     4801 tatacggatc ctgttcagtc aggctccagc ctgaggtctg gcagtgggta cgcagcgcca
     4861 gtgatttcgg gtcctgtgcg ccaaatccac tgaccgcttc gctccataaa taacgtgccg
     4921 cacgcaacat ggcgacgttc ataaacagat ccatgccgat gccgaagaag aacgacaggc
     4981 gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc tgctttgatg tactcaatcc
     5041 catcagcgag cgtaaatgct acctgctgca cgcagttggc acccgcttca cccatgtggt
     5101 aaccgctgat actgatggta ttaaatcgcg gcatgttgcc ggaacaccag gcgatgatgt
     5161 cggcgataat gcgcattgac ggttttggcg ggtaaatata ggtgttgcgg cagaggtact
     5221 ctttgaggat atcgttctga atggtgccgg tcagtttatc aggtgtaaca ccttgctctt
     5281 ccgcggcgac gatataaaac gccagtactg gtagcactgc gccattcatg gtcatcgaaa
     5341 ccgacatttt atccagcggg atctggtcga acaggacttt catatcttcc acggtgtcga
     5401 tagcgacgcc cgctttgccg acgtcgcccg ccacgcgcgg gttatcggag tcgtagcctc
     5461 ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg cccggcggcc aggttacggc
     5521 gataaaaagc gttggactct tttgctgttg aaaaaccagc atactgacgg atggtccacg
     5581 gttgggcggt atacatagtg gcacgcgggc cacgaacgta gggcggcaaa ccaggaaggg
     5641 tacctgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg atggtgatcc
     5701 cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc aattccttgt
     5761 tggcaagctg ttgccactcc tgcacgttag acattatttg ctccgtaaaa cgcaatcaaa
     5821 atgagggtat ttgcgcctac ggtgagcttt ttacgtgggt ttgtcgccag caaaaaaaat
     5881 gcattcagac gtcgtttttt gccgcctgag atttgtctat tttgtgatgt gattcaagga
     5941 atacaaaaag aagtgaggac aacgcctgat atgtgctatc aggcattatt tgatggatta
     6001 atcctgacga aggactttgt gaccataatc gagcaacgca tcagtgactt tacgcatcat
     6061 gcggctttca ggagcaaagc ggtgccagta gagcatccgg cgttgaaata gccctggcgt
     6121 taagtcaatc agttcaccgc tggctagttc tttctcgatt tgcaggtgcg ggatcataca
     6181 gcaggtggtg ccctggcgag caagttgcac gaacgcttct gaagaattaa cgatatggca
     6241 gggcacgctg cctggaggca gatcgaagtt ttgctgcaaa aaggcctggt gcatatcgtc
     6301 aagatggtca aaggcgacca caggtgcttt caataatgcc gaacgcgtta cgccgttagg
     6361 gaaatatttt tcggcaaacg gttttgagct gacgaacaga tagtcaagcg caccgagctt
     6421 atcgacaagg caactcggca gcgcctgatg ttgaatactc accgcgccga ccacttcgcc
     6481 gcggcgcaga cgttcctgag tacgggtttc atcttctacc tgcaagttga ggcggataag
     6541 cgaatcagcc aatacaggag ccagtgccgg aagcaaccac gtcgccagac tgtcggcgtt
     6601 gaccgccagt gaaagcagca gcggagtcga accggtttgt tcatcgccca gccactcttc
     6661 ttccagcaac tccacctggc gcagcagtgc cagcagtttt tgcccttgtt ccgtcgggcg
     6721 cggcggtacg gtacgcacca acagcggctg cccgaacata ttttccagtt gcttaatgcg
     6781 ctgtgagacg gctgattgtg taatgcacag cttttgtgcc gcgcgctcaa atcctcgttc
     6841 acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc gggcgtttca ttgctgttgt
     6901 ctgctcctgg taaatgcttt ttcctgcact atgacacaat tttgtgtcag gttgcatata
     6961 ccgcagccgt cggcgggtaa catttgtatc aatggttaaa cgcgcagctt aaaagatcag
     7021 ggatttgcgc tattgcattt cgcttagcga cttcgcgccc tggttatgtt gaatggcgtg
     7081 gcgttattgc ctctgttcgc accgtggagc ggcataccca gaccgttgta tgctttattt
     7141 gcggggcgaa aggggatgcc cgccattgcg cgatatttta tggatgagtt aaccacccgg
     7201 cttgccaacg gggtctgaat cgcttttttt gtatataatg cgtgtgaaat ttcataccac
     7261 aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca gtaggatggg cggcacttca
     7321 gtatgttcag cccggcacca ttgttggtgt aggtacaggt tccaccgccg cacactttat
     7381 tgacgcgctc ggcacgatga aaggccagat tgaaggggcc gtttccagtt cagatgcttc
     7441 cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat ctcaacgaag tcgacagcct
     7501 tggcatctac gttgatggcg cagatgaaat caacggccac atgcaaatga tcaaaggcgg
     7561 cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt gcggaaaaat ttatctgtat
     7621 tgccgacgcc tccaagcagg ttgatattct gggtaaattc ccgctgccag tagaagttat
     7681 cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa ctgggcggtc gtccggaata
     7741 ccgtcagggc gtggtgaccg ataatggcaa cgtgatcctc gacgtccacg gcatggaaat
     7801 ccttgacccg atagcgatgg aaaacgccat aaatgcgatt cctggcgtgg tgactgttgg
     7861 cttgtttgct aaccgtggcg cggacgttgc gctgattggc acacctgacg gtgtcaaaac
     7921 cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa aaaattctct tcattaaatt
     7981 tggtgacatg tgtcacgttt ttacctggct attgtcgatt gctctaaata aatcctctaa
     8041 accagcatat tcaaccaaga aatacctttg cgtgatattt cctcaacatc gcgacgcaaa
     8101 cgttcatatt gccgcaatat tattttttga tatgttgaaa ggcggatgca aatccgcaca
     8161 caacatttca aagacaggat tgggtaaatg gcaaaggtat cgctggagaa agacaagatt
     8221 aagtttctgc tggtagaagg cgtgcaccaa aaggcgctgg aaagccttcg tgcagctggt
     8281 tacaccaaca tcgaatttca caaaggcgcg ctggatgatg aacaattaaa agaatccatc
     8341 cgcgatgccc acttcatcgg cctgcgatcc cgtacccatc tgactgaaga cgtgatcaac
     8401 gccgcagaaa agctggtcgc tattggctgt ttctgtatag gaacaaacca ggttgatctg
     8461 gatgcggcgg caaagcgcgg gatcccggta tttaacgcac cgttctcaaa tacgcgctct
     8521 gttgcggagc tggtgattgg cgaactgctg ctgctattgc gcggcgtgcc ggaagccaat
     8581 gctaaagcgc accgtggcgt gtggaacaaa ctggcggcgg gttcttttga agcgcgcggc
     8641 aaaaagctgg gtatcatcgg ctacggtcat attggtacgc aattgggcat tctggctgaa
     8701 tcgctgggaa tgtatgttta cttttatgat attgaaaata aactgccgct gggcaacgcc
     8761 actcaggtac agcatctttc tgacctgctg aatatgagcg atgtggtgag tctgcatgta
     8821 ccagagaatc cgtccaccaa aaatatgatg ggcgcgaaag aaatttcact aatgaagccc
     8881 ggctcgctgc tgattaatgc ttcgcgcggt actgtggtgg atattccggc gctgtgtgat
     8941 gcgctggcga gcaaacatct ggcgggggcg gcaatcgacg tattcccgac ggaaccggcg
     9001 accaatagcg atccatttac ctctccactg tgtgaattcg acaacgtact tctgacgcca
     9061 cacattggcg gttcgactca ggaagcgcag gagaatatcg gcctggaagt tgcgggtaaa
     9121 ttgatcaagt attctgacaa tggctcaacg ctctctgcgg tgaacttccc ggaagtctcg
     9181 ctgccactgc atggtggtcg tcgtctgatg cacatccacg aaaaccgtcc gggcgtgcta
     9241 actgcgctga acaaaatctt cgccgagcag ggcgtcaaca tcgccgcgca atatctacaa
     9301 acttccgccc agatgggtta tgtagttatt gatattgaag ctgacgaaga cgttgccgaa
     9361 aaagcgctgc aggcaatgaa agctattccg ggtaccattc gcgcccgtct gctgtactaa
     9421 ttccccttct ctgaaaatta acgggcaggt cactgacttg cccgtttttt tatcccttct
     9481 ccacaccgtt gccgcgttgc gcattctact ttgcgagtct gctccagaaa ataaatcaaa
     9541 gtcagatcaa tgcgttgtgt ttatttaatg gggatttacg atggcgggac agcatggggc
     9601 tgtaacggta aagccctcac cgaagcgagg gcttaaagga gaagggttat gatgcgactt
     9661 gtcatcatac tgattgtact gttactgata agtttcggag cttattaaca gtcagtctct
     9721 ggggaggagt catcctccct tacccttact cactaaatta ggtcaaagaa tcaacgatgt
     9781 caatcagggc gatgcggggt gcatcgccct gaaccgaact ttttgatagc cacttcatcg
     9841 cccacgttta ctgccgtaat agatcctttt tcccaaaaat tatctctgtt ttgcatgggg
     9901 tttttcgaac acgctcgaag aaaagattta aaggtagatc aatgcgttgt ggttatttaa
     9961 agagagctta cgatagctgg gcagcatggg gctgtaacgg taaagccctc accgaagcga
    10021 gggcttgaag gagaagggtt atgatgcgac ttgccatcat actgattgta ctgttactca
    10081 taagtttccc agcttattaa cagtcagtca caggggagga gcaatcctcc cttaccctta
    10141 ctcactaaat taggttaaag aatcaacgat gtcaatcagg gcgatgcagg tgtatcgccc
    10201 ttaccactcc cagactttcg acggtgtaac caccgcagga agagggatat cccactcttc
    10261 aacggggagt ttttccacca actgacaatc atgcgcataa cccaccggtt gcgttttata
    10321 gtgctgccag ttttgtaagg tccgatcata aaaaccaccg cccattccca ggcgctgacc
    10381 gtactcatca aaggcgacca gcggtgtgat cagcacgtct aatcgggaaa ggggtagcac
    10441 gtcacgcaca tccagttttg gctcatggat tttcaacctg ttcatcacca gttcgctttg
    10501 cggatggtaa ttcaggaaca gcaaattacc agcactaaag ggatgcaaaa ctggaagata
    10561 tacgcgctta ccggcgcgcc agagttgttc tattagcggc tgggtgtcga gttcaccatc
    10621 aaaagagagg aatacagcga ccgtatgcgc catcaccacc gggggataag tcatcatccg
    10681 ggtagcggct tgttgaccca tttcctgctg ttgttccggc gttaacgcac gacgacgttg
    10741 ccgaatcatt ttgcggattt cttgtcggga taatgtcagt gggagttctg gtagttgcgt
    10801 catggctggt gccagataag aagggaatct ccgagatgcc gccgcaggct gtaacccttg
    10861 aacccttggt tcaaggtgaa tgtgtcgtcg cagttttaag gcttctcgga cggaccgagc
    10921 atgctcacca accgcggagc gccacattct tgtggtatga aatatcggct caggggactg
    10981 gcccgcttgc gaacatctca gagaaatttt gtcttcacgg ttactctacc acagtaaacc
    11041 gaaaagtgtc attcaaagtt ttggttagtt ttttcggtga tgcgaccttg ttcaagtaac
    11101 gcttgttcta tggtctgctg cagcatccga atacgctgtt ccatacttgc cgcgtagtca
    11161 cgagtcttcg ctttttcttg cgctaactca tagctgatat tcaatgcggc aatgaagacc
    11221 aactgttcag tatttgtgac tctagtgcgt tctttcagat cttgcaaccg ttggttcaga
    11281 tcgtccgctg cctgattcaa cgcatccctt tggtcaggcg ggcagttcac acgcagtgaa
    11341 cggccaaaaa tttggatatc gacgggttgt gcagacatgc caccttcctg ctgattgact
    11401 gcgctgcctt cgtctttaga cccatggtct gcgaaggggc gacactatag ctaccctgat
    11461 gagaagagac aagccctttt ctggtccacc agggcccaaa gtggtagcat atcatgaata
    11521 ttcctccctt tgacgacgaa tgcttatgtc tatacagaac gaaatgcctg gttacaacga
    11581 aatgaaccag tatctgaacc aacaagggac gggtctgacc cctgccgaga tgcatggttt
    11641 aatcagtggg atgatatgtg gcggtaacga tgacagctca tggctgccgc tacttcacga
    11701 cctgacgaac gaaggcatgg ctttcggtca tgagctggca caggcactac gtaaaatgca
    11761 ctctgccacc agcgatgccc tgcaggatga cggcttcctt tttcagcttt atctgcctga
    11821 tggcgatgat gtcagcgttt tcgatcgggc tgatgcattg gcaggttggg tcaatcactt
    11881 cctgcttggt cttggcgtta cgcaaccgaa gctggataaa gtgaccggcg aaaccggtga
    11941 agctatcgac gatctgcgta acattgcgca actgggttac gacgaagacg aagatcagga
    12001 agagcttgaa atgtcgcttg aagagatcat cgaatacgtt cgtgttgccg cgctgttatg
    12061 ccacgacacc tttactcatc cgcaaccgac cgcgccagaa gtacaaaaac cgactctaca
    12121 ctaaaaacaa aaaacgtaag gagagtgtta tgagtgagat atcccggcaa gagtttcagc
    12181 gtcgccgtca ggccctggtg gagcaaatgc aacccggcag cgccgcgctg atttttgctg
    12241 caccagaagt aacacgtagc gccgacagcg aataccccta tcgtcagaac agtgacttct
    12301 ggtacttcac cggctttaac gaaccggaag cggtgctggt gctgattaaa agcgatgaca
    12361 ctcataacca cagcgttctg tttaaccgcg ttcgcgacct gacggcggag atctggtttg
    12421 gccgtcgctt aggccaggat gccgcgccag agaaactggg cgttgaccgc gcactggcat
    12481 tcagcgaaat caatcagcaa ctttatcaac tacttaacgg cctggatgtg gtttaccatg
    12541 cccagggcga atatgcatat gctgatgaaa tcatgaacag tgcgctggaa aaactgcgta
    12601 aaggttcgcg gcaaaatctc accgcaccgg caacgatgat cgactggcgt cctgttgttc
    12661 atgaaatgcg cctgttcaaa tcgccagaag agattgccgt actccgccgc gcgggagaaa
    12721 tcaccgccat ggcacataca cgggcgatgg aaaaatgccg tccgggaatg ttcgagtacc
    12781 atctggaagg cgaaattcac cacgaattta accgccacgg tgcgcgctat ccgtcctata
    12841 acaccattgt cggcagcggt gaaaacggct gcattctgca ctacaccgaa aacgagagtg
    12901 aactgcgcga cggtgacctg gtgttgattg acgcgggttg tgaatacaaa ggttacgcgg
    12961 gcgatattac ccgcaccttc ccggtcaacg gcaaattcac ccaggcccag cgtgaaatct
    13021 acgacattgt gctggagtct ctcgaaacca gcctgcgcct gtatcgtccg ggaacttcca
    13081 ttctggaagt cactggtgaa gtggtgcgca tcatggttag cggcctggta aaactcggca
    13141 ttctgaaagg tgatgttgat gaactgatcg ctcagaatgc ccatcgtcct ttctttatgc
    13201 atggccttag ccactggtta gggctagatg tccatgacgt tggcgtttat ggtcaggatc
    13261 gctcgcgcat tctggaaccg ggcatggtac tgaccgtaga gccagggctg tatattgcgc
    13321 cggatgcaga agtgccagaa caatatcgcg gtatcggcat tcgtattgaa gacgacattg
    13381 tgattaccga aaccggtaac gaaaacctca ccgccagcgt ggtgaaaaag ccggaagaaa
    13441 tcgaagcgtt gatggctgct gcgagaaagc aatgagcgta atcatcgtcg gtggcggcat
    13501 ggcgggcgcg acgctggcgc tggctatttc ccggttaagt cacggggcgc tgccggtaca
    13561 tttgattgaa gcgactgcgc cagagtcaca tgctcatccg ggctttgatg gacgagcgat
    13621 agcgctggcg gcgggtacct gtcagcaact ggcgcgcatc ggcgtctggc aatctctggc
    13681 ggattgcgca actgccatca ccaccgtgca tgtcagcgat cgtggtcacg ctggatttgt
    13741 caccctcgcc gcagaagatt accaactggc ggcgctggga caggttgtcg aattgcacaa
    13801 tgtcgggcaa cggctgtttg cattgctgcg taaagcacct ggcgtaacgc tgcattgccc
    13861 tgatcgcgtg gctaacgttg cccgtactca gagtcacgtt gaagtgacgc tggagagtgg
    13921 cgagacgctg acgggccgcg tgctggtagc agctgatggc acccattcag cgttagccac
    13981 cgcgtgcggc gttgactggc agcaggagcc ttacgaacaa ctggccgtga ttgccaacgt
    14041 tgcgacttcc gttgcgcacg aaggccgcgc ttttgaacgc ttcacgcaac atggcccgct
    14101 ggcgatgttg ccaatgtctg acggacgctg ttcgctggtc tggtgtcatc cactggaacg
    14161 gcgcgaagag gtgctgtcgt ggagcgacga gaagttttgc cgtgaactcc agtcggcctt
    14221 tggctggcga cttggaaaaa ttacccacgc cggtaaacgc agtgcttatc cgctggcgtt
    14281 aacccgcgcc gccagagcta ttacccatcg taccgtgctg gtgggcaatg cggcgcaaac
    14341 tctgcacccg attgccgggc aagggtttaa cctcggtatg cgagatgtca tgagccttgc
    14401 ggaaaccctg actcaggcgc atgagcgcgg agaagacatc ggtgattacg gcatattgtg
    14461 ccgctatcag cagcgtcgac agagcgatcg cgaagcaacc atcggcgtca cggacagcct
    14521 ggtacatctt tttgccaacc gttgggcacc gctggttgtc gggcgcaaca tcggactgat
    14581 gacgatggaa ttattcaccc cggcacgcga tgtgctggcg cagcgcaccc tcggttgggt
    14641 ggcgcgttga aggcggtttt aaggaataaa caatgcaaag tgttgatgta gccattgttg
    14701 gtggcggcat ggtggggctg gcggttgcct gcggcttaca ggggagcggc ttacgcgttg
    14761 ccgtactgga gcagcgcgta ccggaacctc tggcggcgga tgcaccacca caactgcgcg
    14821 tttcggctat caatgccgcc agcgaaaaat tactcacccg tcttggcgtc tggcaggaca
    14881 ttctctcccg tagggccagt tgttatcacg gtatggaagt gtgggacaaa gacagttttg
    14941 gtcacatttc gtttgacgat caaagcatgg gctatagcca tcttgggcat atcgttgaaa
    15001 actcagtgat tcactacgcg ctgtggaaca aagcgcagca gtcgtcagat atcactctgt
    15061 tggcccccgc agaattacag caggtcgcct ggggagaaaa tgaaactttc ctgacgctga
    15121 aagatggcag catgttaacg gcgcgtctgg tgattggcgc ggacggtgct aattcctggt
    15181 tgcgcaacaa agcagatatt tcgctgactt tctgggatta tcagcatcac gcgctggtag
    15241 cgaccattcg cacggaagaa ccgcatgatg cggtggcgcg gcaggttttc catggcgaag
    15301 gcattctggc ctttttaccg cttagcgatc cgcatctttg ctcaattgtc tggtcactgt
    15361 cgccagagga agcgcagcgg atgcagcagg caagtgaaga tgaatttaat cgcgcgttaa
    15421 atatcgcttt tgataatcgc ctgggcttat gcaaggttga gagcgcgcgt caggtgttcc
    15481 cactgacggg gcgttatgcg cgccagtttg ccgcgcaccg tctggtgcta gtgggcgacg
    15541 ccgcgcatac cattcacccg ctggcggggc agggagtaaa tctcggcttt atggatgctg
    15601 cagagctgat tgctgaactg aaacggttgc atcgtcaggg gaaagacatc gggcagtaca
    15661 tttacctgcg ccggtatgag cgtagccgca agcacagtgc agcgctgatg ctggctggta
    15721 tgcagggatt ccgcgatctg ttttccggta ccaatccggc gaaaaaactg ctgcgtgata
    15781 ttggtttgaa actggccgac acgcttcctg gcgttaaacc acaacttatc cgccaggcaa
    15841 tgggattaaa cgatttgcct gaatggctgc gttaaaaatt tctcttctgc tgtttatttg
    15901 atacccatca cactttcatc tcccggtttt ttcgccggga gattttcctc atttgaaata
    15961 aactaatttc acctccgttt tcgcattata ttttctaatg tcattatttt ttgatttagt
    16021 gttttttgac atttttttag ctcttaatat tgccttattc aaattagctt tctaatggca
    16081 tcatctttat atagaaactg gtgtgttttt tggtttttta ttctgtcgcg attttggcgt
    16141 tttttaacca taagctaatg tgatgatcaa ttttacctta tggttaacag tctgtttcgg
    16201 tggtaagttc aggcaaaaga gaacgattgc gttggggacc gggagtggct ccgatgctgg
    16261 gtttcgtggt gataatttca ccatgaaaag ttgtcagccc cgcttattca atgaggacaa
    16321 gatggcacaa cagactcctt tgtacgaaca acacacgctt tgcggcgctc gcatggtgga
    16381 tttccacggc tggatgatgc cgctgcatta cggttcgcaa atcgacgaac atcatgcggt
    16441 acgtaccgat gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc tccgcggcag
    16501 ccgcacccgg gagtttctgc gttatctgct ggcgaacgat gtggcgaagc tcaccaaaag
    16561 cggcaaagcc ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag atgacctcat
    16621 cgtctactac tttactgaag atttcttccg cctcgttgtt aactccgcca cccgcgaaaa
    16681 agacctctcc tggattaccc aacacgctga acctttcggc atcgaaatta ccgttcgtga
    16741 tgacctttcc atgattgccg tgcaagggcc gaatgcgcag gcaaaagctg ccacactgtt
    16801 taatgacgcc cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg tgcaggcggg
    16861 cgatctgttt attgccacca ctggttatac cggtgaagcg ggctatgaaa ttgcgctgcc
    16921 caatgaaaaa gcggccgatt tctggcgtgc gctggtggaa gcgggtgtta agccatgtgg
    16981 cttgggcgcg cgtgacacgc tgcgtctgga agcgggcatg aatctttatg gtcaggagat
    17041 ggacgaaact atttctcctt tagccgccaa catgggctgg actatcgcct gggaaccggc
    17101 agatcgtgac tttatcggtc gtgaagccct ggaagtgcag cgtgagcatg gtacagaaaa
    17161 actggttggt ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc cggtacgctt
    17221 taccgatgcg cagggcaacc agcatgaagg cattatcacc agcggtactt tctccccgac
    17281 gctgggttac agcattgcgc tggcgcgcgt gccggaaggt attggcgaaa cggcgattgt
    17341 gcaaattcgc aaccgtgaaa tgccggttaa agtgacaaaa cctgtttttg tgcgtaacgg
    17401 caaagccgtc gcgtgattta cttttttgga gattgattga tgagcaacgt accagcagaa
    17461 ctgaaataca gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac ttacaccgtt
    17521 ggtattaccg aacatgctca ggagctgtta ggcgatatgg tgtttgttga cctgccggaa
    17581 gtgggcgcaa cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt aaaagcggcg
    17641 tcagacattt atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc actgagcgat
    17701 tccccggaac tggtgaacag cgaaccgtat gcaggtggct ggatctttaa aatcaaagcc
    17761 agcgatgaaa gcgaactgga atcactgctg gatgcgaccg catacgaagc attgttagaa
    17821 gacgagtaac ggctttattc ctcttctgcg ggagaggatc agggtgagga aaatttatgc
    17881 ctcaccctca ctctcttcgt aaggagagag gttcacaatt cactgcacgt ttcaggaacc
    17941 atcgctcatg acacagacgt taagccagct tgaaaacagc ggcgctttta ttgaacgcca
    18001 tatcggaccg gacgccgcgc aacagcaaga aatgctgaat gccgttggcg cacaatcgtt
    18061 aaacgcgctg accggccaga ttgtaccgaa agatattcag cttgcgaccc cgccgcaggt
    18121 tggcgcaccg gcgaccgaat acgccgcact ggcagaactc aaggctattg ccagtcgcaa
    18181 taaacgcttc acgtcttaca tcggcatggg ttacaccgcc gtgcagctac cgccggttat
    18241 cctgcgtaac atgctggaaa atccgggctg gtataccgcg tacactccgt atcaacctga
    18301 agtctcccag ggccgccttg aagcactgct caacttccag caggtaacgc tggatttgac
    18361 tggactggat atggcctctg cttctcttct ggacgaggcc accgctgccg ccgaagcaat
    18421 ggcgatggcg aaacgcgtca gcaaactgaa aaatgccaac cgcttcttcg tggcttccga
    18481 tgtgcatccg caaacgctgg atgtggtccg tactcgtgcc gaaacctttg gttttgaagt
    18541 gattgtcgat gacgcgcaaa aagtgctcga ccatcaggac gtcttcggcg tgctgttaca
    18601 gcaggtaggc actaccggtg aaattcacga ctacactgcg cttattagcg aactgaaatc
    18661 acgcaaaatt gtggtcagcg ttgccgccga tattatggcg ctggtgctgt taactgcgcc
    18721 gggtaaacag ggcgcggata ttgtttttgg ttcggcgcaa cgcttcggcg tgccgatggg
    18781 ctacggtggc ccacacgcgg cattctttgc ggcgaaagat gaatacaaac gctcaatgcc
    18841 gggccgtatt atcggtgtat cgaaagatgc agctggcaat accgcgctgc gcatggcgat
    18901 gcagactcgc gagcaacata tccgccgtga gaaagcgaac tccaacattt gtacttccca
    18961 ggtactgctg gcaaacatcg ccagcctgta tgccgtttat cacggcccgg ttggcctgaa
    19021 acgtatcgct aaccgcattc accgtttgac cgatatcctg gcggcaggcc tgcaacaaaa
    19081 aggtctgaag ctgcgccatg cgcactattt cgacactctg tgtgtggaag tggccgacaa
    19141 ggcgggcgta ctggcgcgtg ccgaagcggc tgaaatcaac ctgcgtagcg atatcctgaa
    19201 cgcggttggg atcacccttg atgaaaccac cacgcgtgaa aacgtaatgc agcttttcag
    19261 cgtgctgctg ggcgataacc acgggctgga gatcgacacg ctggacaaag acgtggctca
    19321 cgacagccgc tctattcagc ctgcgatgct gcgcgacgac gaaatcctca ctcatccggt
    19381 gtttaatcgc taccacagcg aaaccgaaat gatgcgctat atgcactcgc tggagcgtaa
    19441 agatctggcg ctgaatcagg cgatgatccc gctgggttcc tgcaccatga aactgaacgc
    19501 cgccgccgag atgatcccaa tcacctggcc ggaatttgcc gaactgcacc cgttttgccc
    19561 gccggaacag gctgaaggtt atcagcagat gattgcgcag ctggctgact ggctggtgaa
    19621 actgaccggt tacgacgccg tttgtatgca gccgaactcc ggcgcacagg gcgaatacgc
    19681 gggcctgctg gcgattcgtc attatcatga aagccgcaac gaagggcatc gcgatatctg
    19741 cctgatcccg gcttctgcgc acggaactaa ccccgcttct gcacatatgg caggaatgca
    19801 ggtggtggtt gtggcgtgtg ataaaaacgg caacatcgat ctgactgatc tgcgcgcgaa
    19861 agcggaacag gcgggcgata acctctcctg tatcatggtg acttatccct ctacccacgg
    19921 cgtgtatgaa gaaacgatcc gtgaagtgtg tgaagtcgtg catcagttcg gcggtcaggt
    19981 ttaccttgat ggcgcgaaca tgaacgccca ggttggcatc acctcgccgg gctttattgg
    20041 cgcggacgtt tcgcacctca acctgcataa aactttctgc attccgcacg gcggtggtgg
    20101 tccgggtatg ggaccgatcg gtgtgaaagc gcatctggca ccgtttgtac cgggtcatag
    20161 cgtggtgcaa atcgaaggca tgttaacccg tcagggcgca gtttctgcgg caccgttcgg
    20221 tagcgcctct atcctgccaa tcagctggat gtacatccgc atgatgggcg cagaagggct
    20281 gaaaaaagca agccaggtgg caatcctcaa cgccaactat attgccagcc gcctgcagga
    20341 tgccttcccg gtgctgtata ccggtcgcga cggtcgcgtg gcgcacgaat gtattctcga
    20401 tattcgcccg ctgaaagaag aaaccagcat cagcgagctg gatattgcca agcgcctgat
    20461 cgactacggt ttccacgcgc cgacgatgtc gttcccggtg gcgggtacgc tgatggttga
    20521 accgactgaa tctgaaagca aagtggaact ggatcgcttt atcgacgcga tgctggctat
    20581 ccgcgcagaa attgaccagg tgaaagccgg tgtctggccg ctggaagata acccgctggt
    20641 gaacgcgccg cacattcaga gcgaactggt cgccgagtgg gcgcatccgt acagccgtga
    20701 agttgcggta ttcccggcag gcgtggcaga caaatactgg ccgacggtga aacgtctgga
    20761 tgatgtttac ggcgaccgta acctgttctg ctcctgcgta ccgattagcg aataccagta
    20821 attcactgat tcgactattt tctaaaggcg cttcggcgcc tttttagtca gatgacaaag
    20881 tacaaaagtg ctcagacagt cccctcgccc ctttggggag agggttaggg tgaggggaac
    20941 aggccggcac tggcgcgaat atttaccctc accccggcca tctccctgaa agggcgaggg
    21001 gaaaagcgtg ccaacattga agattgagcc agtttgttag caatctcaaa gatacgtcaa
    21061 cgaattaatt tttctcggaa aaacaaatgg ctatagcact tgtgaccggt ggcagtcgcg
    21121 gcatcgggcg ggcaactgca ttactgttgg cgaaagaaga gtatacggtg gcggttaatt
    21181 atcaacaaaa cctccatgcg gcgcaggaag tggtgaactt aatcacgcaa gccggtggca
    21241 aggcattcgt gctccaggcg gatatcagcg acgaaaatca ggtcatcgca atgtttacag
    21301 caatcgatca gcacgatgaa ccgctagcag cgctggtcaa taacgccggg atcttgttta
    21361 cccagtgcac cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt tccaccaacg
    21421 tgacgggata ttttctctgc tgccgcgagg cggtaaaacg catggcgctt aaaaatggtg
    21481 gcagtggcgg cgctatcgtc aatgtctctt cggtggcctc acggttgggt tcgccagggg
    21541 aatatgttga ttacgcggca tcgaaagggg cgattgatac gttaaccacc ggactatcgc
    21601 tggaagtcgc cgcgcagggg atccgcgtta actgcgtgcg gccagggttt atttataccg
    21661 aaatgcacgc cagcggcggc gagcttggac gcgtcgatcg cgttaagtcg aacatcccca
    21721 tgcagcgtgg tggacaggca gaagaggtcg cgcaggccat tgtctggcta ctaagtgata
    21781 aagcctctta cgtcacggga agttttatcg atttggcggg cgggaaataa aacagggaag
    21841 ttgtctgacc ggatgcaaca agtattgcat ccggtacttc atcgacttaa agcttctcgc
    21901 cgttgctggc aatcacctct ttgtaccagt taaagctctt cttacgtgaa cgcgacatat
    21961 cgccagtacc gtcgtcatgt ttattcacat agataaagcc gtagcgtttg ctgtactgcc
    22021 cggtagtgaa tgacacgcag tcgatgcagc cccacggtgt gtagcccatc agatccacac
    22081 catcgtaagt cactgctttt ttcatctctt caatatgggc gcgcaggtag tcaatgcggt
    22141 agtcgtcgtt gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa ccgttttcga
    22201 caataaacag cggcctctga taacgctcat acagttcgca aagggcatag cgcagaccta
    22261 ctggatcaat ctgccagccc cagtcagacg ctttaacata cgggttcggt acgctgcctt
    22321 caaaaccaga gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg atcatgtaat
    22381 agctgaaacc aagataatcg caggtgcctt cacgcagtac atccagatcg ccatcttcca
    22441 ttttgatgtt aaatccgcga cgctcccact cgttcaacac ataggacggg taatagccgc
    22501 gtagctgcac atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg aacatcacat
    22561 cgtccgggtt acaggagtaa ggatagagcg gcaccatcgc cagcatacag ccgactttca
    22621 tctccgggtt aatgcgacgc gcggctttca ctgccagggc gctggcgaca aactggtgat
    22681 gcagcacctg gtacatcgtc tcttccgggt tgtcatgctc ggtatacacc acgccggagc
    22741 agcagtaacc aaacagcggc gcacgccagt tacgctggtt gtttatttcg ttgaaggtca
    22801 tccagtattt gactttatgc ttgtagcgtt cgaataccac ttccgcgaaa cggacaaaga
    22861 aatcaacgac tttacggttg gtccagctac cgtattgctg caccagatgc agcggtattt
    22921 caaagtggga gagggttatc accggttcga tgttgtattt cagcagttca tcgaacagag
    22981 agtcgtaaaa cttcagccct tcttcgtttg gctgagcttc atcacctttt ggaaaaatac
    23041 gcgtccaggc gatggaagtc cgaaaacatt tgaagcccat ttcggcaaat agcttgatgt
    23101 cttccttgta gtgaccataa aaatcaacgg cttcatggtt tggatagtat tttcctggca
    23161 agacttcttt ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga acgtcacaaa
    23221 tgctcggtcc tttgccgcct ttgttccagc cgccttcgac ctgatgagcg gcaacggcgc
    23281 caccccataa gaaatctttc ggtaaggtga gttttttcac tatcataggc tccagatgag
    23341 tcaattctta caggaaatag taccatgcag ccaaacgaca tcactttttt tcaacgtttc
    23401 caggatgaca ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc tgaatcgcac
    23461 ttcaaaacgg gtgatgtgct tcgtgtcgga cgttttgaag atgacggtta tttttgcacg
    23521 attgaagtca ccgcaacctc aaccgtaacg ctggatacgc tgacggaaaa acatgcagag
    23581 caggaaaata tgaccctgac tgaactgata aaggtcattg ccgacatcta tcccggtcag
    23641 acacagtttt atgtgattga atttaaatgt ctttaaattg aactcacgcc tgttagttga
    23701 aatttctcat ttaatttcat gatttttagg agattagtct caaatcagaa tttattttag
    23761 ctaacaggtg ttcactggaa ctattctcag ttacgctgga gaggtataca tgtgcgtgta
    23821 cgtttctccg gagtaagtta tggttcagaa gcccctcatt aagcagggat attcgctggc
    23881 agaggaaatt gccaacagcg tcagtcacgg cattgggttg gtgtttggta tcgttgggct
    23941 ggtgttgcta ctggttcagg cggtggatct caatgccagc gccacagcga taaccagcta
    24001 cagcctctat ggcggcagta tgatcctgct gttcctcgct tcgacgctct atcacgccat
    24061 tcctcatcag cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta tttacctgtt
    24121 gattgccgga acctacacgc cgtttttgct ggtggggctg gattctccgt tagcgcgcgg
    24181 gttgatgatt gttatctgga gcctggcatt gctggggatt ctgtttaaac tgaccatcgc
    24241 gcaccgattc aaaattttat ctctggtgac ctatctggcg atgggctggc tgtcgctggt
    24301 ggtaatttat gaaatggcag ttaagctcgc ggcgggcagc gttaccttac tggcggtagg
    24361 cggcgtggtt tattcgctcg gagtgatttt ctatgtctgc aaacgcattc cctacaatca
    24421 tgccatctgg cacggcttcg tgctcggcgg cagtgtgtgc cactttctgg cgatctattt
    24481 gtatattggg caggcgtaat gggtatgcct ttgtaggcct gataagacgc gtcagcgtcg
    24541 catcaggcga taaatgcctg atgcgataca gagatttact cttcgagtga atacggcagc
    24601 ggctcgatat gcagtgtatt cgcatcgtcg cgtacgcgga agatgctgtc cggttccata
    24661 tcgttattca tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc
    24721 accgtaccgg tacgacgcca gttctcgccc attttcagct ctaagtcttc accagcttcc
    24781 ggcagtcggc tggcgctacc tgccagcaac cagagcgcac gtttattggc accacggaat
    24841 tttgctcgcg ccaccatctc ttgtccggta taacagcctt tcttaaaact gataccgccc
    24901 agcgcctgga gattggtcgc ctgtgggata aactgcccgc tgttcgcggc atcaatcacc
    24961 gggaaacccg cttcaatgtt taatgccagc cactgttggc tattgttcag ttccgcttca
    25021 ccgcgcagtt tatcggtcag catattggca gtagcttcat cggttacgat caggaaacgt
    25081 tctgccgggt gttcaaacca tagcaaagtg gtcgcgcctt ctttaactac ctgtttttct
    25141 ctcgaaggta gttcactaaa gagattggcg agcgcggcgc gcgcctgaaa accggcaaca
    25201 ccaagcagca cacgctcgtc gtctggcgcg atggtcactt tagagaacac cgcatatttt
    25261 ttcagttcag tcagctgcgg ttcacgcacg ctgcgccgtt caatccacgc aaagccatcg
    25321 ccgtcgcgga acagacgtaa attgctccac attttacctt tggcgtcgca atgggcggcg
    25381 agcaggtgct gattttctgt catctggctg acatctgctg tcacctgacc ctgcatatat
    25441 ttttcgctgt ccgcgccagt aatggtggca agcgcccagt catcaagcgt catcagcgtc
    25501 agcggtaaac gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata
    25561 gagtcctgag ttgcttaacg gcgtattaat aactaatggt aaaagagcta tgaggcaatg
    25621 caagtgcttt cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagct
    25681 cttgaattgt tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa
    25741 gaagtcgaaa aacacgttac aatagccggg tactggattt tcgtgagaca ggaagaagat
    25801 ggacattaac aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga
    25861 tatttcaatc atgccgtttt tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg
    25921 catctttatt cgtctgctgg aatgtgacga tccggacctg tttaactggc tgatgaatca
    25981 cggtaaaccg gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg
    26041 ggaacgtggt cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag tggctttcct
    26101 tgctgattca tgggctggtt gccgctgtta ttttactcat gccctggccg ctcagttaca
    26161 ccccgttatg gatggtgtta ctttcgctgg tggtatttga ttgcgttcgc agccagcggc
    26221 gtattaatgc tcgccagggg gaaattcgat tgttgatgga cgggcgtttg cgttggcaag
    26281 ggcaggagtg gagcatcgtc aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt
    26341 tacgttctga tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag
    26401 ctgaatggcg ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaatatgac
    26461 ggcaaagcat atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg
    26521 aggatttgct cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca
    26581 tccagcgcca gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat
    26641 tcatatcctt ccgttggcca gtagccgacg aacttcacgc ctttggtcga gagtttgtca
    26701 tgcagcatac cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca
    26761 tacagcgcaa caattttacc ttcaaggttc aggtcgtcga gctgatccca gacggcttcc
    26821 cagtcttcct ggatttcgcc aaaatcccag gtcgggatac ccagaatgag cacatcgtac
    26881 tgctccatta atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg
    26941 ataatatcgc ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa
    27001 agacccatat tcataacgta agcttctcga ttctgctgtg gcttttgcgg ctagtgtatc
    27061 agaatcgctt tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg
    27121 cgcaagtgaa acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa
    27181 aaaatctggc tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt
    27241 ggttgcatca ccgcgggttg acgctggcga cggcgcaaag tgacgatttg caggcattac
    27301 tggcagaacg gctagagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag
    27361 tgcgccgatt gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc
    27421 atctcgcttc accgaaattg ccacagcgtt tgccaaaaga tttaagcgaa gcgcaggtcg
    27481 aacgtttatt acaggcacca ttaattgatc agccactgga gctacgcgat aaagccatgc
    27541 ttgaagtgtt gtatgctacc gggctgcgtg tctctgaact ggtcgggctg acaatgagtg
    27601 atatcagcct acgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg
    27661 tgccgttagg tgaagaggcc gtttactggc tggaaaccta tctggaacat gggcgtccgt
    27721 ggctgttgaa tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga
    27781 cgcgacagac cttctggcac cgcattaaac attatgccgt gctggcgggt atcgacagcg
    27841 aaaagctgtc accgcatgtg ttgcgtcacg cttttgccac tcatttatta aatcatggtg
    27901 cggatttacg cgtggtgcag atgctactgg gccacagcga tctctccacc acgcaaattt
    27961 atacgcatgt cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt
    28021 gatgtctgaa aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag
    28081 cggcgttttc aggctttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa
    28141 tgggcattaa aagcagcgat attcagccag cacccgtggc tggcatgaag actgtgctga
    28201 ctaacagcgg tgtgttgtac atcaccgatg acggtaaaca tatcattcag gggccaatgt
    28261 atgacgttag tggcgcggct ccggtcaatg tcaccaataa aatgctgcta aagcagttga
    28321 atgcgcttga aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg
    28381 tgtttactga tattacctgt gggtactgcc acaaactgca tgagcaaatg gcagactaca
    28441 acgcgctggg gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gagagccagg
    28501 ctgagcaaca aatgaaagcc atctggtgtg cgaaagataa aaacaaagcg tttgatgatg
    28561 tgatggctgg taaagctgcc actccggcaa gttgcgatat cgatattgcc gatcattacg
    28621 cacttggcgt ccagcttggc gttagcggca ctccggcaat tgtgctgagc aatggcacac
    28681 ttgttccggg ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa
    28741 tgaccagcgg taattaattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga
    28801 tgaaacggca gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg
    28861 gggagtacgc agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca
    28921 actaagcggt gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg
    28981 gattattgtg gtcggtgatt tcgacgccga cggcgcgacc agcacggctc taagcgtgct
    29041 ggcgatgcgc tcgcttggtt gcagcaatat cgactacctg gtaccaaacc gtttcgaaga
    29101 cggttacggc ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg cgcagttaat
    29161 tgtcacggtt gataacggta tttcctccca tgcgggggtt gaacacgctc gctcgttggg
    29221 cattccggtt attgttaccg atcaccattt gccaggcgaa acattacccg cggcggaagc
    29281 gatcattaac cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg
    29341 tgtggcgttt tatctgatgc tggcgctgcg cacctttttg cgcgatcagg gctggtttga
    29401 tgagcgcggc atcgcaattc ctaacctggc agaactgctg gatctggtcg cgctggggac
    29461 agtggcggac gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag
    29521 tcgcatccgt gccggaaagt gccgtccagg gattaaagcg ctgctggaag tggcaaaccg
    29581 tgatgcacaa aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc
    29641 tgccgggcga ctggacgata tgtccgtcgg tgtggcgctg ttgctgtgcg acaacatcgg
    29701 cgaagcgcgc gtgctggcaa atgaactcga tgcgctaaat cagacgcgaa aagagatcga
    29761 acaaggaatg cagattgaag ccttgaccct gtgcgagaaa ctggagcgaa gtcgcgacac
    29821 gctacccggc gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct
    29881 ggcttcgcgc atcaaagagc gttttcaccg tccggttatc gcctttgcgc cagcaggtga
    29941 tggtacgctg aaaggttccg gtcgctccat tcaggggctg catatgcgtg atgcgctgga
    30001 gcgattagac acactctacc ccggtatgat gctgaagttt ggcggtcatg cgatggcggc
    30061 gggtttgtcg ctggaagagg ataaattcga actctttcaa caacggtttg gcgagctggt
    30121 taccgagtgg ctggcccctt cgctattgca aggcgaagtg gtgtcagacg gcccgttaag
    30181 cccggccgaa atgaccatgg aagtggcgca gctgctgcgc gatgctggcc cgtgggggca
    30241 gatgttcccg gagccgctgt ttgatggtca tttccgtctg ctgcaacagc ggctggtggg
    30301 cgaacgtcat ttgaaagtca tggtcgaacc ggtcggcggc ggtccgctgc tggatggtat
    30361 tgcttttaat gtcgataccg ccctctggcc ggataacggc gtgcgcgaag tgcaactggc
    30421 ttacaagctc gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa
    30481 tatctggcca atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc
    30541 gctcttatca ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa
    30601 tccggtaaat aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct
    30661 ttgactacga cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg
    30721 atgtctggaa cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag
    30781 ccgttgtcga caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg
    30841 aactggctgt agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg
    30901 ccctggaaga aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca
    30961 gcgccgactg ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg
    31021 cgagcatgct tgagcgtatg tatttgcgct gggcagaatc gcgtggtttc aaaactgaaa
    31081 tcatcgaaga gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg
    31141 gcgattacgc ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaga
    31201 gcccgtttga ctccggcggt cgtcgccaca cgtcgttcag ctccgcgttt gtttatccgg
    31261 aagttgatga tgatattgat atcgaaatca acccggcgga tctgcgcatt gacgtttatc
    31321 gcgcgtccgg cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattaccc
    31381 acatcccgac cgggatcgtg acccagtgcc agaacgaccg ttcccagcac aagaataaag
    31441 accaggccat gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg
    31501 ctgagaaaca ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt
    31561 cttatgtcct tgatgactcc cgcattaaag atctgcgcac cggggttgaa acccgcaata
    31621 cgcaggccgt gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt
    31681 tatgaggaac caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta
    31741 acaatgaact gaaaacgcgt cgtgagaagc tggcgaacct gcgtgagcag ggaattgcct
    31801 tcccgaacga tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggta
    31861 aagagaacga agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga
    31921 cccgccgtat tatgggtaaa gcctcttttg ttaccctgca ggacgttggc ggtcgtattc
    31981 agctgtacgt tgcccgtgac gatcttccgg aaggcgttta caacgagcag ttcaaaaaat
    32041 gggatctcgg cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac
    32101 tgtctatcca ctgcaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggaca
    32161 aattccacgg cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct
    32221 ctaacgatga atcccgcaac acctttaaag tgcgttcgca gatcctctct ggcattcgcc
    32281 agttcatggt aaatcgcggc tttatggaag ttgaaacgcc gatgatgcag gtgatcccag
    32341 gcggtgccgc tgcgcgtccg tttatcaccc atcataacgc gctggatctt gacatgtacc
    32401 tgcgtatcgc gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat
    32461 tcgaaatcaa ccgtaacttc cgtaacgaag gtatttccgt acgtcataac ccagagttca
    32521 ccatgatgga actctatatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat
    32581 cgctgttccg tactctggca caggatattc tcggtaagac ggaagtcacc tacggcgacg
    32641 tgacgctgga cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat
    32701 atcgcccgga aaccgacatg gcggatctgg acaacttcga ctctgcaaaa gcgattgctg
    32761 aatctatcgg catccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct
    32821 tcgaagaagt ggcagaagca catctgattc agccgacctt cattactgaa tatccggcag
    32881 aagtttctcc gctggcgcgt cgtaacgaca ttaacccgga aatcacagac cgctttgagt
    32941 tcttcatcgg tggccgtgaa atcggtaacg gctttagcga gctgaatgac gcggaagatc
    33001 aggcgcagcg cttcctggat caggttgccg cgaaagacgc aggtgacgac gaagcgatgt
    33061 tctacgacga agattacgtc accgcactgg aacacggctt accgccgaca gcaggtctgg
    33121 gaattggtat cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta
    33181 ttctgttccc ggcgatgcgt ccagtaaaat aagcattacg ttatgctaat aaccccgaca
    33241 aatgtcgggg ttttttaatt gtgctgcgcg aaagcagaca acaattttct tgcttcacta
    33301 ttggctgcct gcattaccat ccacggactg aacgcccacg gcgtggcatc aataccgtgt
    33361 aatacatctg ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac
    33421 gcactgttgg tgcgtgcggc aaataccgga cacacttcat tttccacaat gccattcgga
    33481 tcggtggcgc gatagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca
    33541 agctcataac ggcaacggcg gatcactgcg tcttcgttgc tttctcccag ttgtgggtgc
    33601 ccacaaaccg agttagtcca cacgccaggc catgcttttt tgctcagtgc gcggcgggta
    33661 actaataatt gccccttggc attaaacagc caactggaga acgcgagatg taagagggtg
    33721 tctgccgtgt gtgcggcata cttttccagc gtacccgtgg gaactccctg tgcattcaat
    33781 aaaatgacgt gttccgtttg cataatttct cacatgtaat tctgatcac
//
LOCUS       DAJTMK010000024        33544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTMK010000024 DAJTMK010000000
VERSION     DAJTMK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33544
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.024"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            529..666
                     /locus_tag="OO135_000986"
     CDS             529..666
                     /locus_tag="OO135_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396922.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO135_000987"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO135_000987"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5396923.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAF"
     gene            complement(2991..3920)
                     /gene="virB"
                     /locus_tag="OO135_000988"
     CDS             complement(2991..3920)
                     /gene="virB"
                     /locus_tag="OO135_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5396924.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4137..4373)
                     /locus_tag="OO135_000989"
     CDS             complement(4137..4373)
                     /locus_tag="OO135_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5396925.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4377..6278)
                     /gene="ipaA"
                     /locus_tag="OO135_000990"
     CDS             complement(4377..6278)
                     /gene="ipaA"
                     /locus_tag="OO135_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5396926.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6287..7285)
                     /gene="ipaD"
                     /locus_tag="OO135_000991"
     CDS             complement(6287..7285)
                     /gene="ipaD"
                     /locus_tag="OO135_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5396927.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSNK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKDKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLDNLGGNDEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7336..8427)
                     /gene="ipaC"
                     /locus_tag="OO135_000992"
     CDS             complement(7336..8427)
                     /gene="ipaC"
                     /locus_tag="OO135_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5396928.1"
                     /translation="MEIQNTKPAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8447..10189)
                     /gene="ipaB"
                     /locus_tag="OO135_000993"
     CDS             complement(8447..10189)
                     /gene="ipaB"
                     /locus_tag="OO135_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5396929.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTKLSELDPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10195..10662)
                     /gene="ipgC"
                     /locus_tag="OO135_000994"
     CDS             complement(10195..10662)
                     /gene="ipgC"
                     /locus_tag="OO135_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5396930.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="OO135_000995"
     CDS             complement(10720..11346)
                     /gene="ipgB1"
                     /locus_tag="OO135_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5396931.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="OO135_000996"
     CDS             complement(11362..11751)
                     /gene="ipgA"
                     /locus_tag="OO135_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5396932.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="OO135_000997"
     CDS             complement(11764..13248)
                     /gene="icsB"
                     /locus_tag="OO135_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5396933.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSTESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHPPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEE
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13562..15178
                     /locus_tag="OO135_000998"
     CDS             13562..15178
                     /locus_tag="OO135_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5396934.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNHLNNKSWEPINKNINY
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKGLNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKNLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15188..15550
                     /gene="ipgE"
                     /locus_tag="OO135_000999"
     CDS             15188..15550
                     /gene="ipgE"
                     /locus_tag="OO135_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5396935.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15550..16008
                     /gene="ipgF"
                     /locus_tag="OO135_001000"
     CDS             15550..16008
                     /gene="ipgF"
                     /locus_tag="OO135_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5396936.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LTYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16017..17132
                     /gene="mxiG"
                     /locus_tag="OO135_001001"
     CDS             16017..17132
                     /gene="mxiG"
                     /locus_tag="OO135_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5396937.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICRFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDYKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17140..17391
                     /locus_tag="OO135_001002"
     CDS             17140..17391
                     /locus_tag="OO135_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR5396938.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTAALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17404..17697
                     /gene="mxiI"
                     /locus_tag="OO135_001003"
     CDS             17404..17697
                     /gene="mxiI"
                     /locus_tag="OO135_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5396939.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17703..18428
                     /gene="mxiJ"
                     /locus_tag="OO135_001004"
     CDS             17703..18428
                     /gene="mxiJ"
                     /locus_tag="OO135_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5396940.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEYLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18425..18952
                     /locus_tag="OO135_001005"
     CDS             18425..18952
                     /locus_tag="OO135_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5396941.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18894..19589
                     /gene="mxiN"
                     /locus_tag="OO135_001006"
     CDS             18894..19589
                     /gene="mxiN"
                     /locus_tag="OO135_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5396942.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19582..19989
                     /gene="mxiM"
                     /locus_tag="OO135_001007"
     CDS             19582..19989
                     /gene="mxiM"
                     /locus_tag="OO135_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5396943.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDENQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19973..20401
                     /gene="mxiM"
                     /locus_tag="OO135_001008"
     CDS             19973..20401
                     /gene="mxiM"
                     /locus_tag="OO135_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5396944.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCVLKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20531..21163
                     /gene="mxiE"
                     /locus_tag="OO135_001009"
     CDS             20531..21163
                     /gene="mxiE"
                     /locus_tag="OO135_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5396945.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21150..22850
                     /gene="mxiD"
                     /locus_tag="OO135_001010"
     CDS             21150..22850
                     /gene="mxiD"
                     /locus_tag="OO135_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5396946.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNNNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22869..23936
                     /gene="mxiC"
                     /locus_tag="OO135_001011"
     CDS             22869..23936
                     /gene="mxiC"
                     /locus_tag="OO135_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5396947.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23949..26009
                     /gene="mxiA"
                     /locus_tag="OO135_001012"
                     /gene_synonym="virH"
     CDS             23949..26009
                     /gene="mxiA"
                     /locus_tag="OO135_001012"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5396948.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26022..26423
                     /gene="spa15"
                     /locus_tag="OO135_001013"
                     /gene_synonym="spaK"
     CDS             26022..26423
                     /gene="spa15"
                     /locus_tag="OO135_001013"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5396949.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26427..27719
                     /gene="spaL"
                     /locus_tag="OO135_001014"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26427..27719
                     /gene="spaL"
                     /locus_tag="OO135_001014"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5396950.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27812..28150
                     /gene="spa13"
                     /locus_tag="OO135_001015"
                     /gene_synonym="spaM"
     CDS             27812..28150
                     /gene="spa13"
                     /locus_tag="OO135_001015"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5396951.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28137..29015
                     /gene="spa32"
                     /locus_tag="OO135_001016"
     CDS             28137..29015
                     /gene="spa32"
                     /locus_tag="OO135_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5396952.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSNQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29009..29890
                     /gene="spa33"
                     /locus_tag="OO135_001017"
                     /gene_synonym="spaO"
     CDS             29009..29890
                     /gene="spa33"
                     /locus_tag="OO135_001017"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5396953.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29895..30545
                     /gene="spa24"
                     /locus_tag="OO135_001018"
                     /gene_synonym="spaP"
     CDS             29895..30545
                     /gene="spa24"
                     /locus_tag="OO135_001018"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5396954.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30560..30820
                     /gene="spaQ"
                     /locus_tag="OO135_001019"
     CDS             30560..30820
                     /gene="spaQ"
                     /locus_tag="OO135_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5396955.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30820..31590
                     /gene="spa29"
                     /locus_tag="OO135_001020"
                     /gene_synonym="spaR"
     CDS             30820..31590
                     /gene="spa29"
                     /locus_tag="OO135_001020"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5396956.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31597..32625
                     /gene="spa40"
                     /locus_tag="OO135_001021"
                     /gene_synonym="spaS"
     CDS             31597..32625
                     /gene="spa40"
                     /locus_tag="OO135_001021"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5396957.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32638..32880
                     /locus_tag="OO135_001022"
     CDS             32638..32880
                     /locus_tag="OO135_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396958.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10486 a         4859 c         6707 g        11492 t
ORIGIN      
        1 ttgttgactg cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atctccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt gacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tttaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg ggggcataac
     2101 aaatgatatt actgagtaca aatttagcta tattgggcgg attagttgtg tgggaagacg
     2161 aaatgtttct cttgaaataa gtgaactccc tcaaggaatg attgtgtgct taaatattta
     2221 agtgctggta agtttgagaa ggctgacata ttttgcagag caaacagtga ttgacttgga
     2281 tgatattttt attttagtca ctcgttgtaa ttggagtatg ctttatttta tttgaaaata
     2341 gatgtatttt atacccgcaa tttaaactgt aagccctatg aggggtatgg atattataag
     2401 ttactctttg actaagcata acttttgggg ttagaaaaat aaatctcttg ttaataatta
     2461 atatgatttt aaaatcaaac ttgtttataa ttataaaata atgtaagtcg gtcattttat
     2521 gggaagatta ggagaataaa acagtaatta tatttgagaa agctgctaat atattcttaa
     2581 agcttttttg atactgtgaa cgaagtctgt taatggtgga ttttttgttt atcaccatgt
     2641 aaaaaaattg agtagtaaaa gcgttgactg ctaatcctcg ctgtttttat tgaaacgatt
     2701 aaaaatacgc tgaatatttt cggccagtca ctcatcttca cataaaattt tgaccatcat
     2761 gccgcatccc ttagttgcac aattcatttt caatgatatg aatattacta atttcaccag
     2821 ataatgttct cgtggtcagc agggtatgag ttgtcaccgt aattttaagg aaacacagtg
     2881 cgtggggatt gaataacaga tagccgacgg aattacccca ccggctgaaa gtcaagttca
     2941 acgaagattt ttattatctg aattgggcag tttacatcag tgttcgatgt ttatgaagac
     3001 gatagatggc gagaaattat atcccgaata gcttcatcta tttctctctg aactgttttg
     3061 tttattttag ataatgtaaa tattagagtt ctgtttgttt tattctcttt tctttttatg
     3121 taagtccgtt tgtctttaga aataaataac gagtcaactt ttagcgaagg gttttgcttt
     3181 ctgtttttgc ttttctttat gatgtttaaa atttcttttt tatatatgtc cgggggcaaa
     3241 tttgtatcaa ggtcttttat ttcttccttt aatattggta gtgtagaact aagagattca
     3301 ttagcctttt caagtccttt ataataattg aataatatct tgtagtcatt aaagttaagt
     3361 tctgacgcga ttggaaatag agatattatt tcctgtggaa cgcttgctgc ctgaaaggca
     3421 cgagtgactt tcgcgcgaga cagattctct tttttggcta tgtctttata ggacatccct
     3481 gatactttca gaaaattaag accaatacca agttctcgga tgctatgctc ttttgctgtt
     3541 tgtatatcgt ttgctagttt tctggcatca agagtagata tatattcttt tgaatataga
     3601 acttcaagat ctgctcctgc atatattgca gatgctcttc tacgagtgcc atccagaatt
     3661 tcaattctac catcaatctc ccttcctatt acagggaaga attgttgtag ctttatagtt
     3721 tttatgatat cggctaatga ttcttctgtt agggattctt gatcgcgtac attcgttttt
     3781 tggttgacga aggttaaatc ttggatcctt ttatgaggaa tcttggcttt gataaaggaa
     3841 actttattac cagaatggag tgtaaactct ttcttttggc cttcctttat acttaacaag
     3901 tcgttgcaca aatccaccat atcacaccct gtttattcat attgatttat attgacaaaa
     3961 gttaaatgca gtgtgagcaa gattgtatgt aactgttgat gatagtcaac ggtagtctta
     4021 aaaattacta ttttaatagc tggattgatg caatttatct ttattggata aaagaataat
     4081 attcttgtgg agctctgatg tgagagctcc atagatgaaa aaatgcttat caattactat
     4141 attgattttg tactattttt gactacagaa ataagatctc caatagtaat aatgtgttct
     4201 aatgcagatt catctatacg tagattgaac tcaaaactaa tcatatccag catttcaatc
     4261 agagaaagag aatcggcaga aagatcttca ataaatttag tttcttcaga tagtttgctg
     4321 tacgctatac catagcaaaa ggccactatt gataatattt tttcttttat catattttaa
     4381 tccttattga tattctttaa tacttttgat agggaagtgg ttacatcttt tgctgcttta
     4441 tatatattat tgttttcagc tgtaatatca ttgttatttt ttaaatcaga tatatcatct
     4501 gtaagtaatt ctgcagaggt atcgtcaatt tttgacaata ccttgctgag ggcgctagat
     4561 acttcttttg ccttttcata gatcgcatga tttttatcaa ttgtatctgc gttggtttct
     4621 gttacgtagt ttattgtatt tgcatctgta acttttaatt tgttgtttat tctgtgttct
     4681 gttgtggatt ttgtaatgtc ctttgatttt tcctgtaact ctcgttcttg tgttcctgat
     4741 ttagttccaa tgagagatat aagattactt agggagtttg ttactttttt tgaagcttca
     4801 aatatcgttt cccgtgttaa atatgtgtct ccatttcttg ataaaaaatc agtctgaagt
     4861 acttttgaat cagtgtttga gttgaattta ttgatttgcg aagctactct atgagaaaga
     4921 gaagcgctac ttttagttgt atctgatgag ttatcattac tgcttgtcac tccttccggg
     4981 tggagagggg gggtggttga agagttctgt atgttcagag gcctgtgtcc ccgagaaaga
     5041 gagttatttg tagatctttc ttgactactc gtagttaaca ggtctgaacc tattccatcc
     5101 gaattatctg gagtatggtt gaaaaccttt tctaaagctg aattatttgc aaatgtctgg
     5161 acagtatctg atatagagtg tggtagaacg gaagttactt tttgaactag tgctctttgt
     5221 ggatttagta atgagtttcc gttacgggat aatagatcgt ttgtctgaga agtaggagac
     5281 tgtgcatcat tttctgggtt ctcgtgatag ctattgtcat atactctgtt gtcaaaaact
     5341 ctattatcgt aagttctatt atcattattt atatgatagt gaattacagg ttgggatgtt
     5401 ttctcattgg ctccgggggt tggtccgcca tctggcatat cttcgggagg tgtagggagg
     5461 ccatcttgga taggatgcga atcttttata tcttctaaaa tatcattaag gacttttctc
     5521 aacagctcag gaaacacggc tactgtattt aagtctcttg acagtttacc tataccaaat
     5581 cctttactat caatagtatt tttacgttga ttgcgcaaac tatcaaaggc aagttttgcc
     5641 tgtgcgtcaa ctaaatcatc tagtttgaga gatgaaacag tttcattact taactttaat
     5701 tcagcaagaa tattattctt gataaatgat gatacttcag tattgaggag tttttgtccc
     5761 aatgataaag cttcaccaga tgtgtgttgt tgtgctaata gtgcaagtct gtgctcaatg
     5821 ttttgtcgta attgatggag tttcttattc tttgccgacc atggtccaaa ggcagaatct
     5881 tgggataatt tatcttttat aattagttcc gaatactgtg cgacaatttt ttgaagtccc
     5941 ttttcgtttt caaaattaag accattttga tgtactaagg agtacaaaac atttgaaaat
     6001 tctagaatct cgcttttggc atacgcattc attgagttgg aataaatgct tcttaacaca
     6061 tcttctttcc ctgaggaaga aaataaaaaa tcaagacatt tctgattaaa agacgttgca
     6121 aagttttctt tttcagacac tgatgctggt gtttttagag aagtttgcga actatggata
     6181 tcggatattt tgctttttaa ctcgctgtat tctgttgatg atggtgaagt tgccttatat
     6241 aagaatgttg gcgcttgagt attatttaca ttatgcatgg cgcacctcag aaatggagaa
     6301 aaagtttatc tgtatctgta catgagctta ttgtactact caaaaccttt actaaattat
     6361 caaaaatact attggcatta ctgtattttt gaactaaagt ttgaagatta tttttcattg
     6421 tttcatcttc ggcagagaat ccggcattcc atgcctgata ttttgcatta tctagcacaa
     6481 cctcgtcatt tccacctaga ttatctaagc tttttaacat attgtctatt ggggtcatgt
     6541 ttatattgac aacatatccc ccgttttttt tagatacctt gccgattgtt ccacctaatt
     6601 ctgtaagcca tttatttgct tgttccttac taacagtatt atttgctgga tatagcggtt
     6661 tatctttata tttatccttg agctttgtca attcgtcttt aagcgatttg acttgtaatt
     6721 tcacggagtt tccgtcgtta cctccgggag agatccagcc ggcaagactg gaaagaacag
     6781 cgctaaaatc ttgatacatt tgagtatatg aactaacggc atgttcatat actttcagat
     6841 actgttcatt aatattattg attgactttg caattttatc ccacagttct ctgtgagata
     6901 tcatttcata tccatcaagc tcggcttctt tcggggctga atgtaataat tctcttgcgt
     6961 ctttattaat tggatattct ttattggaaa gaatatccaa tagttgagcg gatttgttta
     7021 catccatgct aatctgagat gaatgtaatg ctatttcttc tagcgatgtt ttagtcaacg
     7081 ttttttgtga gagatctttc tttaatgcct gatttgttgt tctgatatta tgaagggtgt
     7141 cgttgagcat agtaagggaa cttacaggat gagaactggt cgttgttttt atatcagaat
     7201 taactgtttc ggttgatgaa ccgttggtat tgtttgggct gaatgatgag gtggaaatac
     7261 tattagtcag agttgttata ttcatggtta tttccttata ggaagagcca tatattatta
     7321 tggctcttgg atatattaag ctcgaatgtt accagcaatc tgactggctg tagaattcct
     7381 tgattggttg atgttgtcaa ttatattcaa taatttttgt attaattgat ttgtcgcttg
     7441 ggatgcttct ttagatactt gggatgcttc ctctgcttgt ttactgctgg cctgactgat
     7501 tagttgttct tcttcttcaa gagcagatgt ataacgccct cctgatgtgg atatattacc
     7561 agcaacggct gatgtctcca ttgttgcacg gccaatgttt tgtttttgct gagcagaaag
     7621 ggtattgagc tcgtaagctc ttctctgggt gtcaatttta tcctgcaaag aaatatcggg
     7681 agaactaaga gaagttttat gttcagttga cagggatata ttatctggtg ccagtttatt
     7741 tttacctaaa atttttgtgc tagagttagt ttgtggtgag gtgatatttg tatctatttg
     7801 tttatttaac cctaatttag aacctgcaag ctctttttca agagattgag cagtggcaag
     7861 gttctttctt aaggctcctt tttgttcgct aatccctgaa tgcgtttttt tggcaccgat
     7921 acccgttata cctacttgtg tgaccgctcc agtaatgctt gatgataggg ctgctaggcc
     7981 ttgccgaaca atgttctctg cagctgattt tgtagcatcg aacgcaatca atgacaattg
     8041 agagcccaat ctagtttctg cagtgcggag agcagaaagt agaacggcta cactaataat
     8101 taaagaaaca gcattagaag aaagactgga aatatccagg gtgttctctg gagtaattgt
     8161 gtgactcatt tctgaaagat ctttcatctt tttgtcagta aggcgagcaa tgatttctga
     8221 atcatgctga acctgctctg ataactttaa aagttgatca tcatttaatg tagttgttaa
     8281 tacgggattt ttaccgacat taagtggaat atgtgctgct aactgctgat aattttgtga
     8341 tttttgtgtt tcggaagaac tttgagtttg ttttgtggat atatctgtat ataaaatcgg
     8401 ggctggtttt gtgttttgaa tttccataac attctcctta tttgtatcaa gcagtagttt
     8461 gttgcaaaat tgcttttgca acatcagttc tattagcctg agagttggac attgaggcta
     8521 acagatctgc aattacttcc tgcaattggc cgaatttttc tattgcgtca ccgatgtatt
     8581 ttgacagttg ttcaacttga tatttcgata atgtcaggtc tgctagattt ttcgacgcgt
     8641 tgttttggaa aacagcagaa gcgacatttc ctcccgcttg tgtggcagag ttaacacttt
     8701 ctcctaataa aactgcttgg tttaaatggg tggaaataat ttgtttggat attacttcat
     8761 cacccgctgc accaagaaat ttatttaatc tggcaacagc attagtgatt aaatcgtcca
     8821 gttgagaaga aaaattcttg agaaactttg gtataaggtc tgtgagggtt ttacctatta
     8881 ttttgccaat attctctgca agttttgctg ctgcctgttt accaacagtg gctacgagaa
     8941 cgactgctgc aaccaagaca agagcgcctg cgattgcccc cagaatagag ccaatcattt
     9001 tggctttttt cgagtcgacg cccaagcctt cgagcatttt tgtaaatgca tctgaaagga
     9061 gtttgattaa gggttcaatg actgctttca tgatcggatt cagggcttgt tccatgaagg
     9121 aattgccggt cgctgcttgt actatagcat ccgtaaccat aagagctaaa ccaacagctg
     9181 ccagtgctag agatgctcct ccagaaaaag ctgctgcaac aacactaacg atagttaaaa
     9241 gtgccccaag tattttccca acacaaccca ttactctgtt gagttcttct gctttacgta
     9301 cttcagcagc atactcatca gattttctct ccatttcagt ttttcttgat tcttggagag
     9361 actggaatag agccagatca ttttttaaag attcttcatt attttttcca actagttgaa
     9421 taaaggttgc catcaattga gtaacactgg caagtccggt taatgatttc tgctgggttg
     9481 atagctgttc agcagatgct gtgtttgaaa atgcagaaaa agagtctatt tctttttcga
     9541 gttgcattga tttatctgta agtttgctat gaattgacag ggttttctgc tcaatcaatg
     9601 tcctgtcttt aactgctgcg tcttttttga tagtgagttg tatttcttcc cgtcttaatt
     9661 ttttattttc tggtgaatct gggtcgagtt cggataattt tgtttgaatt tggttaattt
     9721 tattttctag gtcttttatt ttagaatctg cgttttttag tttattaatt tgtttttcat
     9781 agtctctggt tagtccttca gtttcagata gaagagtatt aattttatca gagaattcta
     9841 ggtttttttg ctgtcttgcc tgttgctggg acttccaggc agtaatttta tttgttaatg
     9901 cagttaaaga tttttcaccg agtatttgaa taaggtttcc aattaaaagc gttagttggg
     9961 aacttgcatt taatgacttt ggtgctttaa gttcagggat taatatattc gaagttgagt
    10021 gtgcattagt tgtattaata tttttgttag cagtaagatc agcaattgta agagaaaata
    10081 atttgttagc tgcatcattt gcagcctgga tagtattgtc tccaagctca gtggaagcca
    10141 atattttggc aagaggcaaa ccagtggttg tggtgcttac attatgcata ataattactc
    10201 cttgatatcc tgaattgcgt ctaagtatga ttgtgctttt atttttaatt tttcatcatt
    10261 gctgtgttga attacgagtt cgaagcactc tttggctttc aagggggctt tcaaccgaag
    10321 ctggcattgt ccagtatgga ataccggtgt atagtcattt tttcctaatg caaaagcgac
    10381 agcataaagg tctgctgctt gttggaactg ttcttttatc tgataaatag ctgcgagtcc
    10441 cataatgtag tctacattgt aaaagtcgta tatacataaa aatctgaaga aaacttcagc
    10501 ttcctctatt cttcctttgt tgtaaaagtc ataagcatat gaataaatgt catccatcat
    10561 atcatcagga attgcattaa tatctttcag tgtagcgcca gagttaattg catcaattac
    10621 tgcagtagag atgctttcat tttcagtgat atttaaagac ataaggtctc ctttttgtct
    10681 gcattacttt aataagtata agatttaata taaacagatt taatttgtat tgctttgacg
    10741 gtatacagcc tttagaacct ctgcgcttat ttctatttgt aaaacttttg ttgattcatt
    10801 aaatatttta tcgtagacgg aactggatac tagatttgta agatccttca tttgtgtgtt
    10861 aaacaaatcc atgtcactgc tataacgttt taaaatcttt gctctgaggc cagatgaggc
    10921 aatctcgttt agtgctgtat gactgataga tgtttgtgct gatttttcat caagttttat
    10981 atccgggaat tttttttcaa tagctgcaaa tatgtttttt ttgttatctg aggtcaaaac
    11041 aactccattg tgtatggcca tttcatctat acttctgttt atcattgaag agacataaga
    11101 ggttcgctct tggctcatcc aacaaaatag ttgattcccc gaattactat cttttatttt
    11161 gtctatgacg ctgtccctga ttttatttat attcaccggg catccaaaat tcttctgagg
    11221 aaagcgttgt tttaagttaa aaacagataa tattttcttt acaagcttat tgtgacttaa
    11281 cgaatcaaat gatgggcgag gaatcgcgaa ttcaacctgt ggaagtattt tgtttagaat
    11341 ttgcatgata ccccctatat gttagttcac ttctgaagtg atgtttgcaa tggttagttt
    11401 aatacaaaat aaaaattcgt acaaagattc aatgagttca tcaggtgaaa gatatttttt
    11461 gtagtgtttc ttggtcaaaa cacattgatg agtatcaggg agaagacaga tagaagaacc
    11521 ataatctcta ctcaaagagt agttaaggat gagtatgtgt tctggattaa taatattttc
    11581 tgtaaaaagt tcatgttcat taagaagtcc ggtcaaaaag atgtattcat cattatctgt
    11641 tatacaaaca ggtatttgtt cttcgagcaa tataaaggct tgatttttat cgttaaaatc
    11701 aagctttgca cctgttattt cataaagttt atcatatagt ttgcgacaca tttctatggc
    11761 cttctatata ttagaatgag agttattcaa taaaagctta ttgacctgta ataagtcgtg
    11821 aatgtaaagt ttggttccaa gatctggcga tttaagagaa ttgtaataat caatagtttt
    11881 tattgacttt tcgataattt cttctttttt atctgttgct ccggttttta attcgtttaa
    11941 tacacgagac atattgctgg gtgctgattt tactaagtat gagtggcaag cgttgatgac
    12001 cgcatcaaag tcttctgaag atacattttc agtaggatgt ttgtaaagtg aaattgataa
    12061 aggcttgaat gtttttgggg ggtggataag gttaatttta ttgaaacttt taagatagct
    12121 acgtcgtaat tgagtcaatc tggaaagatc aaaatcagat tcaatatact tcattatctc
    12181 gttataagac tgattgagac tttcaatgcg ttgcctaact ttatttgcat acgcgtatac
    12241 attgttaggt gtagcaacca atttaacgtc agggagtgga aagtatattt cagaatttcc
    12301 tgctttaaga acattaagtg ccataccagc acagttttca gatttactga ttaatttata
    12361 atttgccaat tgtctgaaag catctgcttt cttttggcat ataaattttg acatgtttgt
    12421 ttcatcaagg ccaaaaaggt tatagctgat tttaccatcc tttgttttat tatcacctga
    12481 taatggaatg tatactttat cagcactgac cccccaataa ccataggggt cttttttttg
    12541 attagctctg ggaaaaaata tttttttgtt gttattttga tcttgttttg aatttaataa
    12601 gtttctaacc tcttggcctg catcaagtct ttcggctgtt tggtctgaaa tcatatttag
    12661 tttgtcttgt ttataggtat cgacactgta acctgagcgt ttttcaaaaa ggtagtttat
    12721 ttctgtatct tttgtaacat tcccgccggg ataccagctg agataggtat gagttggttc
    12781 ctgtagtaaa ttgtttttta tactgatact ggtatgccct aaatgggtta tgcctctatt
    12841 tccccagcaa cgaaacacta aatgtctttc aagatttttg gctgtaccga tgccatgaaa
    12901 actctctgcg gtattcaaca ttaatttgat gtctttacct gtaaaagaat gttgaattga
    12961 atttttgact gcttcatagg cggcaaacgc actttctgta gatttttcgc tagctaatgt
    13021 cttgaggaaa agcaataagg atttttgatt cgctagtcga taatttttta gttgttcatt
    13081 taatatctgt gaatttatct ttgcattaat agtgcttaat gttctgaaaa agagatcctt
    13141 taggttgaat ttctgtgcta ccaatatagg tccatgctct gtatcttcaa cgcgtatagg
    13201 cccttttgta ttgcttgcgt caatgaaatt gctaattttg agaatcatac tttattaact
    13261 ctccattacg tggtgattgt ggggatgtaa aatgtatttg atatataata tattcaatta
    13321 ataaatttag aaacttgagc ctgttaacat aatcaaattt tcttttgctg tacataatat
    13381 gtacctcgtg agcatatgta gtgctggttt catcatgaaa tcccacaagt taaagtgtct
    13441 gatatatcag gctcggagtg ttatagaaaa agagagaacc ctgttgaata agtatctttt
    13501 ttgttgtaaa acgaggtaca aaactgcaat caatgcccca tttatgactg aggataatta
    13561 aatgcacata actaatttgg gattgcatca ggtttcattt caaagcggag attcctataa
    13621 aggcgcagaa gaaaccggaa accaaaaagg tgtaagcgtg atttcatatc aaagagttaa
    13681 aaatggagag agaaataagg gggtcgaagc gcttaatcga ttatatttac agaaccaaac
    13741 aagcttaaca gggaaaggcc ttttgtttgc gcgtgacaga gctgcagttt tttacgaggc
    13801 aataaagttg gcaggtggtg atacgtcaaa aattaaagcc atgatggaac aattagatac
    13861 ctataaactt ggtgaagttg ataaaagaaa tattaatgag cttaataaag taataagtga
    13921 agagataaga gcacagctag gcattaaaaa taaaaaggaa ttgcaatcgg aaataaaaca
    13981 aatatttact aatcatttaa ataataagag ttgggaaccg atcaacaaaa atattaatta
    14041 tcatgggaaa aattatggtt ttcaattaac acccgcctct catatgaaga tagggaataa
    14101 aaatatattt gtcaaagagt ataatggaaa aggaatttgt tgtgcttcta cgagagagag
    14161 taagcatatt gcgaatatgt ggctttcaaa agtggtggat gacgagggga aggaaatttt
    14221 ttcgggtatc agacatggag ttatatcagc ctatggattg aaaaaaaact catctgaaag
    14281 agctgttgca gctcgtaata aagctgagga gttagcaagc gcagcattat atagcagaca
    14341 agaattatta tcacaggctt tgtctggtaa aacagtagat ttaaagattg tttcgacttc
    14401 tcttctgacg ccgaccagtt taactggggg agaggagagt atgttaaaag atcaggtaaa
    14461 ggcattaaaa gggttaaact ctaaaagggg ggaaccaacg aaattattaa ttcggaatag
    14521 tgatggtctt ctgcaagaag taaaggttaa tctaaaagtg gtgacattta actttggtgt
    14581 aaatgaatta gcgctaaaaa tgggcttagg ctggaggaat gttgacaagc ttaatggtga
    14641 atcaatatgt tctttgttgg gggataattt cctcaaaaat ggtgtgattg gaggttgggc
    14701 tgcagaagct atagaaaaaa atccaccatg taagaatgat gtgatatatc tggctaacca
    14761 gataaaagag attataaaca agaatttaca aaaaaatgat aatggagagc cgtataaatt
    14821 gtcacaaaga atggctctat tggcttatac tataggtgct gtaccttgct ggaattgtaa
    14881 gagtgggaag gacagaacag gtatgcagga tgctgaaatt aaaagagaaa taataagaaa
    14941 acatgaaaca gggcagtttt ctcaattaaa tagtaaatta tcctcagaag agaaaagatt
    15001 attttctact attctaatga atagtggtaa tatggaaatc caagagatga atactggtgt
    15061 gcctggaaat aaagttatga aaaaattacc gctatcttcg ttagagctgt cttatgctga
    15121 aagaataggg gaccccaaaa tatggaatat ggtgaaaggg tattcgtcat ttgtataaga
    15181 ggaatatatg gaagatttag cagatgttat ttgccgagcc ttgggtatcc cttcaattga
    15241 taccgatgat caagcaatca tgcttgatga tgatgtgtta atatatattg aaaaagaagg
    15301 agattcaata aatttattat gccctttttg tgcattgcct gaaaatataa atgatcttat
    15361 atatgcgttg agcctaaatt actcagaaaa gatatgttta gctactgatg atgaaggtgg
    15421 aagtctaatt gcgcgattag atcttaccgg gataaatgaa ttcgaggata tatatgttaa
    15481 tacagaatat tatatttcgc gtgtgcgttg gctgaaggat gaatttgcgc gaagaatgaa
    15541 ggggtattaa tgtcccgttt cgtatttatt tttctgtgtt ttatcccata tttagggaga
    15601 gctgattgtt gggataaggc tggtgaaagg tacaacattc catcgagttt gttaaaagcg
    15661 attgcggaaa aagaatccgg atttaataaa tttgcagtca atgttaataa caatggaagt
    15721 aaagattatg gtataatgca aataaatgac tttcattcta aaagacttag agaaatggga
    15781 tatactgagg aaatgctaat tagtcatcca tgtctttctg tacattatgc agcaaagtta
    15841 ttaaatgagt ttatgctgac gtatggaaga gggtgggagg ctgttggtgc ctataatgca
    15901 ggaacatctc cgaaaaagaa aaaagaaaga ttaaagtacg cagaggatat ctataaacgc
    15961 tatttgcgga tagcagcaga gagcaagcag aataatcgaa ggatataaga ggatttatgt
    16021 ctgaggcaaa gaactcaaat cttgcaccat tcaggttatt agtgaaactc acaaacggtg
    16081 tcggggatga gtttccgtta tattatggga ataacttaat tgttctcgga cggacgatag
    16141 aaacacttga gtttggaaac gataatttcc cagaaaatat tattccagta acagatagta
    16201 aatcggatgg tataatttat ttggccataa gtaaagataa tatctgtcga tttagtgatg
    16261 aaaaaggtga acaaattgat atcaattcac aatttaactc atttgaatat gacggagttt
    16321 cttttcaact taaaaatatg atggaggata aatcgagagt tcatatacta aacggaatgt
    16381 ataataaacc ctcggtgttt tttttctttg ctgttattgt tgtgttaatt ataatttttt
    16441 tcctctcttt aaagaaagat gaagtaaaag aaattgctga aattatagat gataagagat
    16501 atggaatagt caacacagga cagtgcaatt atattcttgc cgaaacacaa aatgatgcgg
    16561 tatgggcatc tgtagcctta aataaaaccg gatttaccaa atgcagatat attctggtgt
    16621 ccaacaaaga aataaataga attcaacaat atatcaatca acgattccca tatatcaatt
    16681 tgtatgtgtt gaatttagtg agtgataaag cggaattact ggtattttta agtaaagaaa
    16741 gaaatagttc aaaagatacc gaacttgaga aattgaaaaa tgctctgata gttgaatttc
    16801 cgtatatcaa aaatattaaa ttcaactatc ttagcgatca taatgcaagg gaggatgcta
    16861 aaggtatttt cacaaaggta aatgtccagt ataaagagat ttgtgaaact aataaagtta
    16921 catattctgt cagagaagaa ctgacagatg aaaagcttga gctcattaat agactgattt
    16981 ctgattataa aaatgtatat ggagatcagt atattgagtt ttctgtactt ttgatagatg
    17041 atgattttaa aggtaaatca tatcttaaca gcaaagacag ttatgtaatg ttgaatgata
    17101 aacactggtt ttttttagat aaaaataagt gaggataaaa tgagtgttac agtaccggat
    17161 aaagattgga ctctgagttc attatctgaa acttttgatg atggaactca aacattacaa
    17221 gggcaactaa cagcggcact aaatgcatta gctgaaaatc cttcgaatcc acagttgctg
    17281 gctgaatacc aaagtaaatt atctgaatat acattatata ggaacgcgca atctaatact
    17341 gtgaaagtga ttaaggatgt tgatgctgca attattcaaa acttcagata atagggagca
    17401 ttcatgaatt acatttatcc agtcaatcag gttgatatta tcaaagccag tgattttcaa
    17461 tctcaagaga tatcaagcct ggaagacgtc gtgtcggcta aatatagtga tattaagatg
    17521 gatacagata ttcaagtatc acaaataatg gagatggtaa gcaatccaga atcattaaac
    17581 ccagaatctt tggccaagtt acagacgacg ctctcaaatt attcaatagg agtatcatta
    17641 gctggcacgt tagcaagaaa aacagtttcg gctgttgaaa ctttattaaa gtcttaattt
    17701 atatgattag gtataaaggt tttattttat tcttgttgct gatgttgatt ggatgtgagc
    17761 aacgtgaaga gttaatttct aatttatctc aaagacaggc aaatgaaata atatctgtgc
    17821 tagaacgcca taatattact gctagaaaag ttgatggagg taaacagggg atctcggtac
    17881 aagtcgaaaa ggggacattt gcatcggcag ttgatttgat gcgcatgtac gatttgccaa
    17941 atccggagag agttgatatc tcacaaatgt ttcctacaga ttcattagtg tcttctccaa
    18001 gagctgaaaa ggcccgttta tatagtgcta ttgagcaacg gctggaacag tctttagttt
    18061 ctattggtgg tgttatttcg gcaaaaatac atgttagcta tgatcttgaa gaaaaaaata
    18121 tatcttcaaa accgatgcat atatcagtaa tcgctatata tgactcaccg aaagagtctg
    18181 aactattagt tagtaatatt aagcgatttt tgaaaaacac cttttctgat gttaagtatg
    18241 aaaatatatc tgtcatatta actccgaaag aagaatatgt ttatacaaat gtacaacctg
    18301 ttaaggaaat taaatcggaa tatttaacaa atgaagtaat atatgtattt ctcgggatgg
    18361 ctgtactagt tgtcattctt ttagtatggg cattcaaaac agggtggttc aagagaaaca
    18421 aaatatgata agaatggatg gaatttataa aaaatatctt tcaataattt ttgatccagc
    18481 gttctatata aatagaaatc ggttgaattt gccttctgaa ctgttagaaa atggcgtaat
    18541 cagaagtgag attaataatc tcataattaa taaatatgat ctaaattgcg atattgaacc
    18601 tttaagcggg gtaaccgcta tgtttgttgc caactggaat ttacttccag ctgttgctta
    18661 ttttatcggt agtcaagagt ctcggttaat taatcatagt gagatggtta ttagttatta
    18721 tgggggcaaa atcagtaagc aaggggaggc cgctattcga agtggttttt ggcatcttat
    18781 tgcgtggaag gaaaatatat ctgttggtat atatgaaaga ataaacttat tatttaatcc
    18841 aattgctttg gagggaaatt atacgcctgt agaaagaaat ttgtcccgat taaatgaagg
    18901 tatgcaatat gcaaaaaggc actttaccgg tatccagaca tcatgcctat gatggtgttg
    18961 ttataaagag aattgaaaag gaattatgta aaaccataaa agatagagac actgagagta
    19021 agaagaaagc aatttgtgtt ataaaggatg ctacgaaaaa agctgaatca ctgcgtatag
    19081 atgctgtatg cgatggttat caaattggta tacagactgc ttttgagcat attattgact
    19141 atatttgtga atggaagtta aagcaaaatg aaaacagaag aaatattgag gactatataa
    19201 ctagtttgct ttcagaaaat ttacatgatg agaggatcat tagcacttta ttggagcagt
    19261 ggcttagttc gttgcgtaat acagtaacag aattaaaagt ggttttgccc aagtgtaatt
    19321 tagcactgag gaaaaagtta gaacttgatc tccataaata tcgctcagat gtcaagatta
    19381 ttttaaaata ttctgaaggg aataattata tattctgtag tggtaatcaa gtagtggagt
    19441 ttagtcctca ggatgtaata tcaggtgtta aaatagaact tgcggaaaaa ttaacaaaaa
    19501 atgataaaaa atatttcaaa gagttagcac ataagaaact gagacaaatt gcagaagact
    19561 tgttaaaaga gaatcctgtt aatgattaat caaataaatg caagcaatgc tctgcagcaa
    19621 agattaaata gtgaagaatt tgttaattta aatgagagat tatcatcttc gcaatcattt
    19681 gatgaggata taatctatga aataatgcaa tatttctcac agagtgaatt aaattccata
    19741 gataatgatg aactgcacaa taagattgaa caattattca attcacgttt tccttatctt
    19801 acagctgcac aaaaaagttc acttcttaat aaactcatag atgaaaatca atatgtcgat
    19861 ttgcatgagg gattttatgc atcattgtcc atatataaca atattgattt ctatattaag
    19921 actacaacct tcgattcatt aattagtgtc tttgaagcag ggagagaggc agatgattcg
    19981 acatggtagt aataagttga aaatatttat tttaagtata ttgctattaa cactgagtgg
    20041 gtgtgtttta aagtcatcat ctaattctga aaaagaatgg catattgttc ctgtaagtaa
    20101 ggattatttt tctattccaa atgatttatt atggtcgttt aatacaacca ataaaagtat
    20161 aaatgtttac tctaaatgta ttagtggtaa ggcggtttat agttttaatg caggtaaatt
    20221 catgggcaac tttaatgtta aggaagtaga tgggtgcttc atggatgcac aaaagatagc
    20281 tatagataaa ctattttcta tgctgaaaga cggggttgtt ttaaaaggta ataagataaa
    20341 tgataccatc cttatagaga aggatgggga agttaaatta aaattaattc gagggatata
    20401 attgtattgt gagtaaatat aaaggtctaa atacaagtaa tatgttttac atttactcta
    20461 gtggacatga accagttaac gttgagcttg taaaagataa agaacgtaac ataattgagc
    20521 tggctccagc atggaagggt ttttttttgt ccgaaatcaa aatataaaat tcagtgataa
    20581 tgtgaactat cattatcgct ttaacattaa tagttgtgca aaatttcttg ctttttggga
    20641 ctatttcagt ggtgctctag tagagcatag ccatgctgaa aaatgtattc atttctatca
    20701 tgagaatgat cttcgggata gttgtaatac cgaatcgatg cttgataagt taatgttaag
    20761 attcatcttt tcatctgacc agaatgtaag taatgcactg gctatgatac gaatgactga
    20821 gtcatatcat ttggttcttt atcttctgag aactattgag aaagaaaagg aggtaagaat
    20881 aaagagtctg acagagcatt atggtgtgtc ggaggcttat tttagatctt tatgtagaaa
    20941 agctttaggg gcaaaagtca aagaacaact gaacacttgg cggttagtaa atggtttatt
    21001 agatgtattt ttgcacaatc aaacaatcac ttctgctgcc atgaataatg ggtatgcatc
    21061 aacatcccat ttttcaaatg agataaaaac acgactaggc ttttctgcaa gagaattatc
    21121 gaatattact tttttagtga aaaaaataaa tgaaaaaatt taatattaaa tctttgactc
    21181 tcttgattgt attgttacca ttgatagtta atgctaataa tattgactct catttattag
    21241 aacaaaatga catagcgaaa tatgttgctc aaagtgatac ggtaggatct ttttttgaac
    21301 gtttttctgc gttattaaat tatcctattg ttgttagcaa acaagctgca aaaaagagaa
    21361 tatctgggga gtttgattta tctaatccag aagaaatgct tgaaaaaatg acattacttg
    21421 tgggactcat atggtataaa gatggaaatg cattatacat atatgactct ggagaattaa
    21481 taagcaaagt tattttgctg gagaatatat cacttaatta tcttattcag tatctgaagg
    21541 atgctaacct ttatgatcac agatatccta taagaggaaa cattagtgat aaaacattct
    21601 atatatctgg tcctccggca ttggttgaac tggtagctaa tacagcaact ttgcttgata
    21661 agcaggtttc ttctattggg acagataaag ttaattttgg ggttataaaa ttaaaaaata
    21721 catttgttag tgacagaact tataatatga gaggggagga tattgtcatc ccaggggtgg
    21781 ctactgttgt agaacgttta ttaaataatg gtaaggctct gagtaacaga caagccaaaa
    21841 atgatccgat gccacccttt aatattacac agaaagtatc tgaggatagt aatgactttt
    21901 catttagttc agttaccaat agttctatat tagaggatgt atcacttatt gcatatccag
    21961 aaaccaattc tatattagtc aaaggtaatg atcagcaaat acagattatt cgcgatatta
    22021 tcactcagtt agatgtagcc aaacgacata tagaattatc attatggata atcgatattg
    22081 ataaatctga attaaataac cttggcgtta attggcaagg aacggcttca tttggtgatt
    22141 cgtttggtgc atcatttaat atgagctcat cagcatcgat aagcactttg gacggcaata
    22201 agtttattgc ttcagttatg gcattaaatc agaaaaagaa agcaaatgta gtttcaaggc
    22261 cggtgatttt gacacaagag aatattccgg ctatatttga taataacagg actttttatg
    22321 tgtcattagt tggcgagcgc aattcttcgt tggagcatgt tacgtatgga acattgataa
    22381 atgttatacc tcgcttttca tctcggggac aaattgagat gtctttaacg atagaggatg
    22441 gaacaggaaa tagtcagtca aattacaata ataataatga aaatacatct gtccttccag
    22501 aagttggaag gaccaaaatc agtacaatag cgagagttcc acaaggcaaa agtttgttga
    22561 taggtggtta tacgcatgaa acgaactcta acgaaatagt atctattcct tttctctcct
    22621 ctattcctgt aatcggtaat gtgttcaagt ataaaacaag taatatcagt aatattgtaa
    22681 gggtttttct gattcaaccc cgtgagataa aggagtcaag ttattataat actgcagaat
    22741 ataagagcct catatctgaa agagaaatac aaaagactac acaaataatc ccatctgaaa
    22801 ctactttgct ggaagacgaa aaatcattgg tttcatactt aaattactaa ctataaagta
    22861 ggtgatgtat gcttgatgtt aaaaatacag gagtttttag ctctgcattc attgataagt
    22921 tgaatgcaat gacaaattca gatgatggag atgagactgc tgatgcagag cttgattctg
    22981 gcttggctaa tagcaagtat attgactcat ctgatgagat ggcttccgct ctttcgtcat
    23041 ttataaacag aagagacctt gagaaactga aaggaacaaa tagtgatagt caggaacgta
    23101 ttttagatgg ggaagaagat gaaattaatc acaagatttt tgatttaaag agaactttaa
    23161 aagataacct tcctctagat caggatttta tagacagact aaagagatat tttaaagatc
    23221 caagtgatca agtcttagca ttaagggagc ttttgaatga aaaagatctt actgctgaac
    23281 aagtcgaatc attaactaaa attattaatg agataatatc aggtagtgaa aaaagtgtta
    23341 atgctggaat aaattcagct atacaggcta aattatttgg caacaaaatg aaacttgaac
    23401 cacagctttt gcgtgcatgt tatcgtggtt ttatcatggg gaacatatca acaacagatc
    23461 agtatataga atggcttggt aattttggtt ttaatcacag acatacaatt gtgaattttg
    23521 tagagcagtc actgattgta gacatggatt ctgagaaacc gagctgtaat gcttatgagt
    23581 ttggttttgt gttatctaaa ttaattgcaa ttaagatgat tagaacttca gatgtaattt
    23641 ttatgaagaa actggaatcc tcaagcttgc taaaagatgg cagtttaagt gcagagcagc
    23701 tattgctaac tttattatat atttttcaat atccaagtga aagtgagcaa attcttactt
    23761 ctgttataga cgtatcacga gccagtcatg aggattctgt agtgtatcaa acatatctat
    23821 cttctgttaa tgaaagtcct catgatatat ttaaaagtga aagtgaaaga gaaattgcga
    23881 tcaatattct acgagagctt gtcacaagtg catacaagaa agagctttct agataacagg
    23941 agatgaaagt gatccagtct tttcttaagc aagtaagtac taagcctgaa ttaattatat
    24001 tagtgcttat ggttatgatc attgctatgt tgatcatacc attacctaca taccttgttg
    24061 attttttaat agggcttaat attgtacttg ctattctcgt ttttatggga tcattttata
    24121 ttgaaagaat attgagtttt tctacattcc cttcggtttt gttgataacg acattatttc
    24181 gtctcgcttt atcaataagt actagtcgac taattttagt tgatgcggat gcgggtaaaa
    24241 ttattactac atttggccag tttgtcattg gtgatagtct ggctgtaggt tttgttattt
    24301 tttcaatagt aactgttgtg cagttcattg ttataacaaa aggatctgaa agggttgcgg
    24361 aggtagctgc tcgcttttct cttgacggta tgccggggaa acaaatgagc attgatgcgg
    24421 atttaaaggc tggaattatt gatgccgcag gagctaaaga aagacgcagt attctggaac
    24481 gtgagagtca attgtatggt tcatttgatg gagcaatgaa gttcattaaa ggtgatgcaa
    24541 ttgctggcat cattattatt tttgtcaatt taataggagg tatttctgtt ggtatgagtc
    24601 aacatggaat gtctctatct ggtgcattat ctacttatac catactaact attggtgacg
    24661 gattggtctc tcagattcct gctctgttaa tttctataag tgcaggattt atcgttacaa
    24721 gagttaatgg tgatagcgat aatatgggac gtaacataat gtcccagata tttgggaatc
    24781 cttttgttct cattgttaca tcagctcttg ccttggcgat agggatgttg ccaggttttc
    24841 cgttttttgt ttttttcctg atagcagtta ctttgacggc tttattctat tataaaaagg
    24901 tcgtagaaaa agaaaaaagt ctgtctgagt ctgattctag tggctatact ggtacatttg
    24961 atattgataa tagccatgac tcatctttgg caatgataga aaatctggat gctattagtt
    25021 cagaaaccgt tcctttaatt ttattatttg ccgaaaataa gataagtgca aatgatatgg
    25081 aaggtcttat tgaaaggata agaagtcagt tctttattga ttatggtgtc aggcttccaa
    25141 ctattttata tagaacaagc aatgagctta aggtcgatga tattgttttg ttaataaatg
    25201 aggtgcgtgc cgatagtttt aatatatact ttgacaaagt gtgtattaca gatgaaaatg
    25261 gagatataga tgctctaggt attcctgttg tttcaacttc atataatgaa cgtgttattt
    25321 cttgggtaga cgtttcatat acagaaaatc taactaatat tgatgctaaa attaaaagtg
    25381 ctcaagatga attttatcac cagttgtcac aagctttatt aaacaacata aatgagattt
    25441 ttggtataca agaaacaaaa aatatgttgg atcagtttga aaatcggtat cctgatctat
    25501 taaaggaagt cttccgacat gtgactatac agagaatttc tgaggtatta caaagattgc
    25561 ttggagaaaa tatttctgtt cgcaatttaa aacttattat ggagtctttg gcgctttggg
    25621 ctcccagaga aaaagatgtc ataacattag ttgaacatgt ccgtgcatca ctttctaggt
    25681 atatttgtag taaaatagct gtttctggtg agattaaagt tgtgatgctt tccggatata
    25741 ttgaggatgc aataagaaaa gggataaggc aaacctctgg tggctctttc ttgaatatgg
    25801 atatagaggt ttcggatgag gtaatggaaa ctttagcaca tgctttgaga gaattgagaa
    25861 atgcaaaaaa aaatttcgtt cttttggtat cagtagatat acgtaggttt gttaaaagac
    25921 ttatagataa cagatttaag agtatactcg ttatatcgta tgctgagatt gatgaagcat
    25981 ataccattaa tgtattaaag actatttagt gaggtttaaa tatgagtaac attaatttag
    26041 ttcaattagt tagagatagt cttttcacga ttggttgtcc tccatcaatt attacagatt
    26101 tagacagcca ttcagcgatt accatttctt tggatagtat gcctgctatt aatatagctc
    26161 ttgtcaatga gcaggttatg ttatgggcta atttcgatgc accaagtgat gtaaaacttc
    26221 agtcatctgc ttataacata ttaaatttaa tgttaatgaa ctttagttat agcattaatg
    26281 agctggtaga gcttcatcgt tctgatgagt acttacagtt aagagttgtt attaaagatg
    26341 actacgttca cgatgggatt gtttttgcag agattctgca tgagttttat cagaggatgg
    26401 aaatcttaaa tgaggtctta taatcaatga gctatataaa attgctcact caattatctt
    26461 ttcctaatag aatctcgggg ccaatcttgg aaacaagtct tagcgatgtt tcgattggtg
    26521 agatttgtaa cattcaggct ggaattgaaa gtaatgaaat tattgcaaga gctcaggttg
    26581 taggatttca tgatgaaaaa acaatattaa gcttgattgg aaattctcgt ggactttcac
    26641 ggcaaacgtt gattaagccc actgcccagt ttcttcatac gcaagtcggc cgtggattat
    26701 tgggagcagt agtcaatcct ttaggggagg ttactgataa gtttgctgtt acggataaca
    26761 gtgaaattct ttatcgacct gtagataatg ctcctccgct atatagtgaa agggctgcaa
    26821 ttgagaagcc ttttttaaca ggtgttaagg ttattgattc tttactcacg tgtggtgaag
    26881 gacagcgaat ggggattttt gcgtcagctg gttgtggcaa aacttttctc atgaatatgc
    26941 tcattgaaca tagtggtgct gatatatatg ttattgggtt aattggtgag cgaggtcgag
    27001 aggttacaga aacggttgat tatttgaaaa actctgagaa aaaaagcagg tgtgttttag
    27061 tatatgcaac ttcggattac tcttcggttg atcgttgtaa tgctgcatat atagccactg
    27121 ctatagccga attttttagg actgaaggac ataaagtagc gctttttatt gattcattaa
    27181 caaggtatgc cagagcatta cgtgatgtgg ccttagccgc tggagaatca cctgccagaa
    27241 gaggctatcc ggtttcggtt tttgatagct tacccagact tcttgaaagg ccaggaaagt
    27301 taaaggcagg tggctctatt actgcatttt acactgttct tttggaggat gatgattttg
    27361 ctgatccatt agctgaagag gtaagatcca ttttagatgg acatatatat ttgagcagaa
    27421 atctagccca aaaaggacaa tttcctgcaa ttgattcctt aaaaagtata agcagggtat
    27481 ttacacaggt tgttgatgaa aaacatcgta ttatggccgc tgcatttcgg gagctacttt
    27541 ctgaaataga agagctaagg acaattattg actttggtga atacaaaccg ggggagaatg
    27601 cctctcagga taaaatctac aacaaaatat ctgttgttga gagttttctg aagcaagatt
    27661 atcgactcgg ttttacttat gagcagacaa tggagcttat tggtgaaaca attagataag
    27721 gttttaaaaa tcaaagataa atatcaacgg tctgttaaat taattgaagc acacatatta
    27781 acactaaaaa aaaaactctt tgtatcgaga tgtggaggca ttagataaaa ggattatata
    27841 cttcctgcaa ctggagaatg acttggaacc tgtaggcgct cagagtgtta gtcagctttt
    27901 caatacaaga cgtaagatcg ctatagtaaa aaagcatatt atccaatatc agtctgaacg
    27961 aattcttcta aagggaagaa ttgaagaaat tcaaaaagat attgatgagg caaatgcttc
    28021 taaaagaaaa ctgcttcata aagagagtaa aatatgtaaa cggattggct tgattaaaag
    28081 aaataacttt gccaaacagt tgatacttga tgagttatca caagaggata tgaagtatgg
    28141 cattagataa tataaaccta aattttagta gtgacaaaca gatagaaaaa tgtgagaaac
    28201 tatcttcaat agataatatt gatagtctcg ttttgaagaa gaagaggaag gttgaaattc
    28261 ctgagtactc tttaattgca tctaactatt ttactattga taagcacttt gaacataagc
    28321 atgataaagg agaaatttat agtggcatta aaaatgcgtt cgaacttaga aacgaacgag
    28381 cgacatattc tgatattcca gaatcaatgg ccattaaaga aaatattttg ataccagatc
    28441 aagatatcaa agcaagggaa aaaataaata tcggcgatat gagggggatc ttttcatata
    28501 ataagagtgg aaatgcagac aagaacttcg aaagaagtca tacttcttct gtaaaccctg
    28561 ataatctgct agaatctgat aatagaaatg gtcaaattgg tttaaaaaat catagcttgt
    28621 ctattgataa gaatattgct gacatcattt ctttactaaa tggaagtgtt gctaaatcat
    28681 ttgagctgcc tgtaatgaat aaaaatactg cagacataac cccatccatg tcattgcaag
    28741 aaaaatcaat agttgaaaat gataaaaatg tttttcaaaa aaatagtgaa atgacttacc
    28801 actttaaaca gtggggggct ggacattctg ttagtatttc agtggagtct ggttcttttg
    28861 ttctaaaacc gtcagatcaa tttgtaggaa ataaacttga cttaattttg aaacaagatg
    28921 ctgagggtaa ttacagattt gatagcaatc aacataataa ggggaataaa aataatagta
    28981 caggatataa tgaacagagt gaagaagaat gctaagaatt aaacattttg acgctaacga
    29041 aaaactacag attttatatg caaagcaact ctgcgagcgt ttttcgattc agacattcaa
    29101 aaataaattt acaggcagtg aaagtttagt cactcttact tccgtgtgtg gggattgggt
    29161 aattcgtatt gatacattat cttttttgaa aaaaaaatac gaggtatttt caggattttc
    29221 tacacaagaa tctttactgc atttatcaaa atgtgtcttt atagagtcgt catctgtatt
    29281 ttcgattcca gaactgtctg ataagattac tttccggatc acgaatgaaa tccagtatgc
    29341 aactactggg agtcatttat gctgtttttc ctcttcttta ggtattattt attttgacaa
    29401 gatgccggta ttacgtaatc aagtttctct tgacttattg catcatcttt tagagttttg
    29461 cttaggttca tctaatgtaa ggctggctac tttaaaaaga attcgtactg gtgatataat
    29521 catagttcag aaactttata atttattatt gtgtaatcaa gttattattg gggattatat
    29581 tgtgaatgat aataatgagg caaaaattaa tctgtcagaa agtaatggtg agtcagatca
    29641 cacagaagtt tctttggcat tattcaatta tgatgatatc aatgtaaaag tggactttat
    29701 ccttttagaa aaaaagatga caatcaatga actaaaaatg tatgtagaaa acgaattatt
    29761 caagtttccc gatgacatag ttaaacatgt aaatattaaa gtaaatggtt ctttggttgg
    29821 gcatggggaa cttgtttcta ttgaggatgg ttatggtatc gagattagtt cttggatggt
    29881 aaaggagtaa tctcatgctg agtgatatgt ccctcatcgc tacactttct ttttttacgc
    29941 tattaccatt tttagttgct gctggtactt gttatataaa gttttctatt gtttttgtaa
    30001 tggtacgaaa tgcacttggt ttgcagcagg ttccatcaaa tatgactctc aatggtattg
    30061 cattgattat ggctttgttc gtgatgaaac ccattattga agctggttat gaaaattatc
    30121 taaatggccc gcaaaaattt gatacaatat cagatattgt ccgtttttct gattctggct
    30181 tgattgaata caagcaatac ttgaaaaagc atacagatct tgagttggca cgttttttcc
    30241 aaaggtctga agaagaaaat gcagatttaa agagtgcaga gaataatgat tattcattgt
    30301 tttccctctt gcctgcatat gcgctaagtg aaattaaaga tgcgttcaaa ataggttttt
    30361 atttatattt gccatttatt gttgttgatc ttgttatttc aagcatttta cttgcacttg
    30421 gtatgatgat gatgagtcca atcacaatat cagtaccaat aaaacttgta ttatttgttg
    30481 cgcttgatgg gtgggggatc ctgtctaagg ccttgattga acaatatatc aatattcctg
    30541 cttagatgat ggaggtaatg tgtctgatat agtttatatg ggtaataagg ctctttattt
    30601 aatccttatc ttttccttat ggccagtagg tatagctacg gttattggat taactattgg
    30661 tttattacag acagtgactc aacttcaaga gcagacactt ccttttggta taaagcttat
    30721 aggtgtctca atatctttgc tacttctttc tggatggtat ggtgaggttt tattgtcttt
    30781 ttgtcatgaa ataatgtttt taattaagag tggggtttga tggacatttc aagctggttc
    30841 gaaagtattc atgtgttttt aatactcctg aacggcgttt tttttagatt ggctccatta
    30901 tttttctttc ttccattttt aaataacggt ataatttctc catctattag aatacctgtg
    30961 atttttcttg ttgcatcagg attaattact tctggtaagg tagacatagg ttcttctgtt
    31021 tttgaacatg tttatttcct tatgttcaag gaaataattg ttggtctcct tctctctttt
    31081 tgcttgtctc ttcccttttg gatatttcat gctgttggta gcattattga caaccagcgt
    31141 ggggcaacgc ttagtagttc aattgatcct gccaatggtg ttgatacgtc tgagttggca
    31201 aaatttttca atcttttttc tgcagttgta tttctataca gtggtggaat ggtctttatt
    31261 ttagaatcca tacaattgtc ttataatata tgcccgttat tttctcaatg ttctttccgt
    31321 gtctcaaata tattaacatt tctgacttta ttggcaagtc aggctgttat tttagccagt
    31381 cctgttatga tagtattgtt actatcagaa gtattacttg gtgtattatc gagatttgct
    31441 ccgcagatga atgctttttc cgtatcatta actattaaaa gtttacttgc aatatttatt
    31501 attttcatct gttcttctac tatttacttt tctaaagttc aatttttcct cggtgaacat
    31561 aaatttttca caaatctatt tgttagataa aatattatgg caaataaaac agaaaagccg
    31621 acacctaaaa aactaaagga tgccgcaaaa aaaggacagt catttaaatt taaggattta
    31681 acgacagttg ttattattct ggtagggaca tttactataa tatcattctt ttccttaagt
    31741 gatgtaatgc tcttatacag atatgtaata attaatgact tcgaaattaa tgagggtaaa
    31801 tacttttttg cagtggttat tgtctttttt aagataattg gcttcccact ttttttctgt
    31861 gttctttcgg ctgtgttgcc aacattggtt caaacaaagt ttgttcttgc gactaaagct
    31921 atcaagattg atttttcagt attaaaccct gttaaagggt taaaaaaaat atttagtata
    31981 aagacaataa aagaattttt caaaagcatt ctgcttctta ttattctagc attaacaacc
    32041 tatttctttt ggattaatga ccgaaaaata attttttctc aggtgttttc tagtgttgat
    32101 ggcttatatc ttatttgggg ggggctgttt aaggatataa tattattttt cttagcattt
    32161 tctatttttg ttattattct tgactttgtg attgagttca ttttatacat gaaagatatg
    32221 atgatggata aacaggagat aaaaagagaa tatatagagc aagagggaca ctttgagaca
    32281 aagtcgagaa ggcgtgagtt gcatatcgag attctttcag agcagactaa atctgatata
    32341 cgtaattcaa aattagtggt aatgaacccg actcatattg caattggtat ttatttcaat
    32401 ccagaaatag cgcctgcacc ttttatttct ctcattgaaa ctaaccagtg tgccttggct
    32461 gtcagaaaat atgcaaatga agttggtata ccaactgtgc gtgatgtgaa attagctaga
    32521 aaactatata aaacacatac aaaatatagt tttgttgatt ttgaacactt ggatgaagtc
    32581 ctacgtctta ttgtttggct tgagcaggtt gaaaacactc attaataagt aaaggagatg
    32641 tgttatatgg gagttaattt ctgtaataaa ataggtattg atcagtctga atttgaaata
    32701 gaatcttcca tcattaactc cattgctaat gaggtattga acccaatatc tttccttagc
    32761 aacaaggata taataaatgt tttactcaga aagatttcat ctgaatgtga cctggttaga
    32821 aaagacattt atcgttgtgc tcttgagttg gtcgttgaaa aaacacctga tgatttataa
    32881 tcattcataa taactctttt gctgatataa atatttttgt tgaagatatt tatgtagtca
    32941 caggatcaat tgtagaaaat tgttatgttt ttgttaggaa ctgaactaaa taatatatga
    33001 taagacagca aaaacgactg accataatcc ttcttttatt aggagttgat aagagagact
    33061 attcatcctg taatgttaaa actctgttat atagtattcg cgattatgca aagtctgtaa
    33121 atgataatga gatactaacc gagagtaatc gtttattatc tcattgcatt tcagatagta
    33181 acggagcttt ctttaaaagt agtaaatacg ttccccttaa gtacttacgt aaaaggagaa
    33241 tagcacgaaa gataccaaat gattagcact ttgctagtaa gcttgctggt aaactatatt
    33301 gaccgttggg tttgttttga gactgtaatt taaattgtgt aattgcctgt ttttgatatg
    33361 tttactccaa caacggagac aggtaaatta tggacgaaaa gaagcttaaa gcccttgcgg
    33421 ctgaactggc taaaggcctc aaaactgagg ccgacctcaa tcagttttcc cgtatgctga
    33481 cgaaatatcc cggctcttaa aacgatagtg gctcaggctt tcatttttca gcgttcccca
    33541 gaag
//
LOCUS       DAJTMK010000025        32593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTMK010000025 DAJTMK010000000
VERSION     DAJTMK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32593
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.025"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            522..761
                     /locus_tag="OO135_001023"
     CDS             522..761
                     /locus_tag="OO135_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5396959.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(949..1926)
                     /gene="intI2"
                     /locus_tag="OO135_001024"
                     /pseudo
     CDS             complement(949..1926)
                     /gene="intI2"
                     /locus_tag="OO135_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2258..2731
                     /locus_tag="OO135_001025"
     CDS             2258..2731
                     /locus_tag="OO135_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5396960.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2826..3351
                     /gene="sat2"
                     /locus_tag="OO135_001026"
                     /pseudo
     CDS             2826..3351
                     /gene="sat2"
                     /locus_tag="OO135_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
     gene            3420..3917
                     /locus_tag="OO135_001027"
     CDS             3420..3917
                     /locus_tag="OO135_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396961.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3978..4349
                     /locus_tag="OO135_001028"
     CDS             3978..4349
                     /locus_tag="OO135_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396962.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4759..5136
                     /locus_tag="OO135_001029"
     CDS             4759..5136
                     /locus_tag="OO135_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5396963.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5159..5311
                     /locus_tag="OO135_001030"
                     /pseudo
     CDS             5159..5311
                     /locus_tag="OO135_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5367..6983)
                     /locus_tag="OO135_001031"
     CDS             complement(5367..6983)
                     /locus_tag="OO135_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5396964.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6984..8510)
                     /locus_tag="OO135_001032"
     CDS             complement(6984..8510)
                     /locus_tag="OO135_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5396965.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8513..10180)
                     /locus_tag="OO135_001033"
     CDS             complement(8513..10180)
                     /locus_tag="OO135_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5396966.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10177..12285)
                     /locus_tag="OO135_001034"
     CDS             complement(10177..12285)
                     /locus_tag="OO135_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5396967.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12272..13093)
                     /locus_tag="OO135_001035"
     CDS             complement(12272..13093)
                     /locus_tag="OO135_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5396968.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13253..15082)
                     /gene="glmS"
                     /locus_tag="OO135_001036"
     CDS             complement(13253..15082)
                     /gene="glmS"
                     /locus_tag="OO135_001036"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5396969.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRIGHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTELLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15244..16614)
                     /gene="glmU"
                     /locus_tag="OO135_001037"
     CDS             complement(15244..16614)
                     /gene="glmU"
                     /locus_tag="OO135_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5396970.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILMF
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16964..17383)
                     /gene="atpC"
                     /locus_tag="OO135_001038"
     CDS             complement(16964..17383)
                     /gene="atpC"
                     /locus_tag="OO135_001038"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5396971.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17404..18786)
                     /gene="atpD"
                     /locus_tag="OO135_001039"
     CDS             complement(17404..18786)
                     /gene="atpD"
                     /locus_tag="OO135_001039"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5396972.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18813..19676)
                     /gene="atpG"
                     /locus_tag="OO135_001040"
     CDS             complement(18813..19676)
                     /gene="atpG"
                     /locus_tag="OO135_001040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5396973.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19727..21268)
                     /gene="atpA"
                     /locus_tag="OO135_001041"
     CDS             complement(19727..21268)
                     /gene="atpA"
                     /locus_tag="OO135_001041"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5396974.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21281..21814)
                     /gene="atpH"
                     /locus_tag="OO135_001042"
     CDS             complement(21281..21814)
                     /gene="atpH"
                     /locus_tag="OO135_001042"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5396975.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21829..22299)
                     /gene="atpF"
                     /locus_tag="OO135_001043"
     CDS             complement(21829..22299)
                     /gene="atpF"
                     /locus_tag="OO135_001043"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5396976.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAYKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22361..22600)
                     /gene="atpE"
                     /locus_tag="OO135_001044"
     CDS             complement(22361..22600)
                     /gene="atpE"
                     /locus_tag="OO135_001044"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5396977.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22647..23462)
                     /gene="atpB"
                     /locus_tag="OO135_001045"
     CDS             complement(22647..23462)
                     /gene="atpB"
                     /locus_tag="OO135_001045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5396978.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23471..23851)
                     /gene="atpI"
                     /locus_tag="OO135_001046"
     CDS             complement(23471..23851)
                     /gene="atpI"
                     /locus_tag="OO135_001046"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5396979.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24468..25091)
                     /gene="rsmG"
                     /locus_tag="OO135_001047"
     CDS             complement(24468..25091)
                     /gene="rsmG"
                     /locus_tag="OO135_001047"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5396980.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQRPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25155..27044)
                     /gene="mnmG"
                     /locus_tag="OO135_001048"
     CDS             complement(25155..27044)
                     /gene="mnmG"
                     /locus_tag="OO135_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5396981.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALDGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSHRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27423..27866)
                     /gene="mioC"
                     /locus_tag="OO135_001049"
     CDS             complement(27423..27866)
                     /gene="mioC"
                     /locus_tag="OO135_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5396982.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWVNLLK"
     gene            complement(27956..28414)
                     /gene="asnC"
                     /locus_tag="OO135_001050"
     CDS             complement(27956..28414)
                     /gene="asnC"
                     /locus_tag="OO135_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5396983.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28566..29558
                     /gene="asnA"
                     /locus_tag="OO135_001051"
     CDS             28566..29558
                     /gene="asnA"
                     /locus_tag="OO135_001051"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5396984.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(29563..31014)
                     /gene="viaA"
                     /locus_tag="OO135_001052"
     CDS             complement(29563..31014)
                     /gene="viaA"
                     /locus_tag="OO135_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5396985.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEKPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQLFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31008..32504)
                     /gene="ravA"
                     /locus_tag="OO135_001053"
     CDS             complement(31008..32504)
                     /gene="ravA"
                     /locus_tag="OO135_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5396986.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNCIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         8309 a         8235 c         7561 g         8488 t
ORIGIN      
        1 ggtcggcagc cctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt
       61 tttaaggatt atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag
      121 ctaatacgaa aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttaa
      181 ctaatctagc taacctgccg ctcagcttag tacgatccta tatccaactg atcataatcc
      241 tttcatcagt atcccaaatt aaatccccct cctagactgc ctcaccaaca ctttacagtg
      301 atcccgtatt tccggacagg tgattgattt ttttcgcttg tacaggtgtt aagccacagt
      361 tatcttgatg tggacattat gcattgtaat agcccattaa catgcgctaa gagcataaat
      421 taatatacag taacaagcat tacgaggtgc taatgtttgg aaagcagcct cttccaacta
      481 caataataag ttaactatcc tccacccaca aataattaca aatgacccct ttccgctata
      541 actccgatct caccagcggc tcgctacaaa cccgagagtg tcgcattatt actggcttgt
      601 tactccaaga gcttgatgaa gctgcctggg ataaagccat gtataaggaa aatgtgcttc
      661 agaaacgcac gcaatctacg gtaagacgta tttcttcagc gcttagaaaa cgcctagaac
      721 acctaagctc tgacttttgg gcttttgcgt ttttatgtta ggcattgtct ggcaactgga
      781 ggaagtgtaa tgaccatgaa tggtcactgt gattgacagt atcttgctta aacttatctg
      841 aacaatggta gaactggctc atatttatcc cctctatgat tgtttatctt tttcaccata
      901 aaaagtatca ggggctactc agggctacat aaagatattt atctactttt actgattgat
      961 aagtagcatc agtccatccg caggactggt ggtgccggca aaatgctgac ccaacacatg
     1021 cgtatagatt tgcgtggtct taacatcgtt atgccctaag agttcttgca cagtgcgaat
     1081 atcacgcccc gcttgtaata gatgcgtagc aaacgagtga cgaaatgtat gacaagtgac
     1141 acgcttgcta acgatgcctg ctttttgtac ggctgccttc aatgcctttc gcgcaacgga
     1201 gtcatgcaga tgatggcggc ataatttgcc gttatacggg tggttgcaga gcgtgctgga
     1261 gggaaagaca aacatccacg ccgcttgtcg ataagcagaa gggtatttgt gatctaaagc
     1321 aaaaggcagc gatggcccta cgccttgtaa gttgtcgtct tgctgaataa gccgcgcttg
     1381 ctcaatgagt tattttattg ctgggattag gcgcgtgggc agtaggctgt ttctgctttt
     1441 cccaccctta ccgtcatgca cagtgatgca gccattatca aaatcaaaat ctttaacccg
     1501 caaacgcaag cattcattaa tgcgcaaacc tgcaccatac agcagcgtaa aaataacttg
     1561 gttgcgagta tccataacct gcaaaatgcg ttgcacttca tttgcagaga taacagaggg
     1621 tagccgtcta ggcttgcttg cagggatata atcaatatcg cccaacggct gttgtaaaaa
     1681 cctgttgtac aaaaaagcta gggcatttaa agcgattttc tgcgtgttta tggctacatg
     1741 tctgctgttt gctaagctgg ataaaaacag cctgacctct tcactgccca tggtctgagg
     1801 atgacgtttt ttgtgaaaca gaataaaacg cttaatccag tgcaggtaag ttttttcagt
     1861 tttcagcgca taaccttttt gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt
     1921 agacataaaa cgctccttgt cttgcaactg tctgcctata cagcctattc tagctgggat
     1981 ttaaaaaagt gcctgttttt tacgcctaga gatgcttgtt taccggtaga gttttaattt
     2041 aatgctaaat aaattaaaat gttatgagtt ctttgggtga gataatgtgc atcgtgcaag
     2101 caggatagac ggcatgcacg atttgtaata acagagtgtc ttgtattttt aaagaaagtc
     2161 tatttaatac aagtgattat attaattaac ggtaagcatc agcgggtgac aaaacgagca
     2221 tgcttactaa taaaatgtta acctctgagg aagaattgtg aaactatcac taatggtagc
     2281 tatatcgaag aatggagtta tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga
     2341 acagctcctg tttaaagcta ttacctataa ccaatggctg ttggttggac gcaagacttt
     2401 tgaatcaatg ggagcattac ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac
     2461 atctgacaat gagaacgtat tgatctttcc atcaattaaa gatgctttaa ccaacctaaa
     2521 gaaaataacg gatcatgtca ttgtttcagg tggtggggag atatacaaaa gcctgatcga
     2581 tcaagtagat acactacata tatctacaat agacatcgag ccggaaggtg atgtttactt
     2641 tcctgaaatc cccagcaatt ttaggccagt ttttacccaa gacttcgcct ctaacataaa
     2701 ttatagttac caaatctggc aaaagggtta acaagtggca gcaacggatt cgcaaacctg
     2761 tcacgccttt tgtaccaaaa gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc
     2821 gtcatatgaa gatttcggtg atccctgagc aggtggcgga aacattggat gctgagaacc
     2881 atttcattgt tcgtgaagtg ttcgatgtgc acctatccga ccaaggcttt gaactatcta
     2941 ccagaagtgt gagcccctac cggaaggatt acatctcgga tgatgactct gatgaagact
     3001 ctgcttgcta tggcgcattc atcgaccaag agcttgtcgg gaagattgaa ctcaatctca
     3061 acatggaacg atctagcctc tatcgaacac attgttgtgt cgcacacgca ccgaggcaaa
     3121 ggagtcgcgc acagtctcat cgaatttgcg aaaaagtggg cactaagcag acagctcctt
     3181 ggcatacgat tagagacaca aacgaacaat gtacctgcct gcaatttgta cgcaaaatgt
     3241 ggctttactc tcggcggcat tgacctgttc acgtataaaa ctagacctca agtctcgaac
     3301 gaaacagcga tgtactggta ctggttctcg ggagcacagg atgacgccta acaattcatt
     3361 caagccgaca ccgcttcgcg gcgcggctta attcaggagt tagagagctg gggaagacta
     3421 tgcgcgatct gttgaaggtg gttctaagcc tcgtacttgc gatggcatcg gggcaggcac
     3481 ttgctgacct gccaattgtt ttagtggatg aagctcgtct tccctatgac tactccccat
     3541 ccaactacga catttctcca agcaactacg acaactccat aagcaattac gacaatagtc
     3601 catcaaatta cgacaactct gagagcaact acgataatag ttcatccaat tacgacaata
     3661 gtcgcaacgg aaatcgtagg cttatatata gcgcaaatgg gtctcgcact ttcgccggct
     3721 actacgtcat tgccaacaat gggacaacga acttcttttc cacatctggc aaaaggatgt
     3781 tctacacccc aaaagggggg cgcggcgtct atggcggcaa agatgggagc ttctgcgggg
     3841 cattggtcgt cataaatggc caattttcgc ttgccctgac agataacggc ctgaagatca
     3901 tgtatctaag caactagcct gctctctaat aaaatgttag gcctcaacat ctagtcgcaa
     3961 gctgagggga accactagtg tcatacgaac ctccaagaga cggttacaca aacgggtaca
     4021 ttgttgatgt catgtatgac aatcgcccaa gtaagtatcc agctgtgttc agaacgtacg
     4081 tccgaattcc agactatgtc gaactagata acgcggagga gctggccgac gcaatcattc
     4141 gagaggttga gttaaaggag gcatggctca caagggtctt ggggaactca gccgatcctg
     4201 acgcttctgt gggcaagtgg gaccggcgcc tagcgcagtg cggtggtatt gatgagatgt
     4261 caagtggggt gcaggcaaca tacttcagtc caaacggtca gctggtcggc ttagtggcat
     4321 acattgatgc cgaatggatg gatgaatagc tctgacgtta cgattcaggt gagacaacca
     4381 gatgggcatt gcgtacaagc tcagttgcca ccgatgtggc tacgagtctg atcttcttta
     4441 cctaggccaa ggtatggcga tgcttcctga gcaaatagtc ggaacgtgca catgctgcga
     4501 caatctaacg acgatttcta taaatgaaag ctacggagta tgctcacttt gtggggctaa
     4561 ggatcgtgtg atatttacaa gctatcagca tgaggcaagg ccaagaaatc cctgctatct
     4621 agagagaagt ttcaaatatc aatgcccaag atgccatgcc ttcagtatgg agcctccgga
     4681 tctaccggag atgctttggg actaatgctc cgctttgcct cgggtatccg ttcgacctat
     4741 gtttaagagg aaacgcacgt gtctgccgcc aaagctcttg cctatgcact ttgtgacaag
     4801 ttggaaagca aaaaagttga tgaagtcgta cgaaatcttg tttctagcgg ctggaatatt
     4861 agggaagtgg cctgggaagc ttcgtccttt gatcatgaaa tgccctactt tgacacgggg
     4921 cgggacttcg atattggatt tcctgacacg ctgtcatcca agattgagtt tttgaccgcg
     4981 aatggatcaa tcgtccaagc acgtgctcgc ctgcacttca agcaggctct aatttttgcc
     5041 aaggcgtcca aacatgtcgc cgcgctcaag aactcattgg attactacta tggatcgtct
     5101 gtaatccccc ctcaaaacca cggaggctat ttgtagaaaa ttccattaat atgaactcaa
     5161 ggtgttccct acggacgaat cagcgatgaa ggtggtgtac ctggctatcc aggcggcgac
     5221 caagaaatgg acgatgccga tccgcaactg gaagccggcc atgaaccgtt ttatgatcga
     5281 atttggtgac cgtttaaatg gccaccttta actgaccggc aaaagcactt acacaaaagg
     5341 gtttacaggc tctttcagtc attcctttaa tgcgtaaatt gctctctaac cactctctca
     5401 gcccaccctt ccatatcctc tctcgatact tgcagctccc ccaacccctt tggatgattc
     5461 acccctctat gcccgtcttg tccatataat tgatcaaaca aactatttgg ccaattcaat
     5521 gagctcttca ccactccacg ccgtatcttt tcaaaatcat tccgccatgt ttcgctatca
     5581 acgccactca acactttggt agaaagcatt ttcaccccat ccgatgcatc cacctctagc
     5641 aatacaaatt cactgccatt acgttgcacc ccaacggctt taatcactct tggtgagcca
     5701 tctttacaca gatgtaatcg gctacgccca acttttggca aaaccaaggt ttcttcaaaa
     5761 agcacccgac atgcaaattt agtttttaga attgaaagta gctcatcaaa agcagcaaat
     5821 cgattagcaa aaatagagtt gtaattggtt gcatcctgct tcccacccac atctgccgct
     5881 gctaggacac cacctaagtg tggctcatca gtactaacca aattactatt ttcacttgtt
     5941 gttgcttctt ctttttctaa aatagtttgg ctactttttc ttgccctgcg agatttttca
     6001 attcgactag gtctactaaa acttatccat gtcggctcag cctctatgac tcgtgtgtct
     6061 gtgtctatat tggctgtttc ctcatcgtcc aactgtagat gctcatcatc aacaactgac
     6121 tctgttgaaa ccgctatgtg ctgcgtacta ccatcacctg cctccttttc ctgaaaagag
     6181 gcatggctaa tagctgttgt gctaggaagc atagcgttga tttctaaacc tactatttcc
     6241 tcaactaaat aatccttatc ctcgttagaa gaacgtcctt ttacatgtaa tttccaaccc
     6301 tgcatgggcg gagggtcaaa gctaaagcac cagctttcaa cgccgttctt aatttctcta
     6361 ttttgttgat agtgcctaaa aatactttca tacgaatcca taacatcttg atccgaaaac
     6421 aaccaaacca aaagctgcac tacggccgac tgctctaacg cccctttagg aaacgatgaa
     6481 ctgggtaaga tccttatctc taaatgatct cgctcaacct catactgaac gtcgaactct
     6541 tgctgtaaag cggtactgct caaacagctt cgacagaaat aggagttaat taaaaataat
     6601 gaccgagcta gctctaactg tgggatgtga taacgagtct tgctatatgg tgtttcagct
     6661 tcgtaaataa atgaactctg cgctccatcc ttgttgcgta cggaagcaaa ggacgagaga
     6721 ttaggaaaat cgattatttt ggctcgttga agatccgatg cttcaaactc aatcacacga
     6781 tccgccttat ttatttgctt tgttgtactg ttaaccacct tagctcgact taataaaggc
     6841 aaatgagtaa atcgagtcca tttccgttct tgcattggat taaaccaaac aaaaatacgc
     6901 cactccttat gacccgagtt acggaataaa tgaccaatat gtacaacctg cacattgtca
     6961 ttaaatgtag ctagcctaac cactcactct tccccataaa ccatttccaa gaatctttgt
     7021 gcttcctcag ttatccgctc tttagataac gttgcacttc ttaataatcg ccagcgccta
     7081 agctcaacat cttcctgttt aagcttaata aaagcttggc taatccgtct aatttgataa
     7141 tcttccacac tctctgagta acgctttaag caaagcgcta ccaaaggcag tttctgtaga
     7201 ttttttgcaa gagaggttcc gttaggagtt tgctttaaca gccagctcga tgtcgctctt
     7261 gggtgatcaa ggctactatc tagacgcttt atgattctta atagttgtcg tacagcaatt
     7321 cgatctcttt ggttccaatc aactctaggg gcgggtgcca gacgctcttg ttgatgctgt
     7381 tgattccagt gaactagcca atccctgtca tgtcggtaaa gccaagcgta tagcacccca
     7441 ccctctaaag actgtcttgc cgccttaatt ccttggtatt tatgcactag ttgctgccag
     7501 tctttatgct taacagataa atcttcagag ttaggttgca cagactggct aacaggtctt
     7561 gtcgttatag agtgctcagt aagagcactt gcctgctgta gcgcttctat aaccgttagt
     7621 tttggcaata aggcttgcca cacaatacta tgctgtaaat aactaaaggc ttttctatgc
     7681 ttacggaata tacttttcag ccaacacgtg tccttgttct ctgccaactt taaatccagt
     7741 ttctctagtg cctcatcact aaaagtctgc ctcactctct ccgccaccaa gtcatgacga
     7801 atgtgcttgc ttttggttag ccctagatcc tgcgctaagc gctgataaaa cagcgtccac
     7861 tgctcaaggc ttggggaaag ctcttgcgct cgtggagcat ctaacagagg ggctatataa
     7921 gcagctagtg ctgttaattg agatagggag tctttggggt agtctgaaag cagctcagta
     7981 tgacccaaag cccaaaattg atgtcggtga tcatctacag ctctatcaaa gaagactaaa
     8041 gcaccgtgtt ttggacaata tggcaaagcg ggcaaatacc aatctcgttg ccaaaaggct
     8101 tccccatacc tatttagctg aagagcaacg caatcagggc agtatctaaa gcggttatcg
     8161 ctcttaactc tagaagcagc gactcctagc attaaatgca ccgcaccttg cgcttggtac
     8221 tccattaacc gaatagcttc gtctcggcgc tccttgccta caaacggagc atataaaggg
     8281 aataaggtat gctcataaat aagctgctga acagcgtaac gtcctgtttg atgtagatgt
     8341 cttgctatca cacctaaatg cgagggcaga cctaaggtag cgaccacctt gcggttgcca
     8401 tacacctcat ccaacagctg cttaggacta acaatccctt gataaacgcc tgcccgtgca
     8461 atagtgctat aaatcagctc attcgagtac ggaacaggaa agtttctcat ggctaacctg
     8521 cttgtttaaa taagctcgcc atatccacta ttaccccttt cccttttaac cgttcatgca
     8581 tggttttttc aggttggcgt tgtgaataga tataacgtaa atccgtatca ggcaagctat
     8641 cccagtcgct gggcttgact acctttatag ccgaaacctt tttactcttg gagggctttt
     8701 ctagttctga cactaccgtt tcgccttcca tcaaccactg caaaacaaga ggcagtaact
     8761 tttgtcttgt catcgttgga ttctggctaa acgctttttt aatagtggga attaacaggc
     8821 ttgaatcgta atcctctttc agcatcagat ataaatgacg ctgatcttcg gtatctaact
     8881 cttgaagggc tttttcttct ggtgtttgtt cttgtatcgc tgcgatatct agctgaagtt
     8941 ggattaaccg tttatcaatc tcgggaacga ctagatcaga ataacgagca atgcgttctg
     9001 ggatacccga gcgtaatgcc tctagcatgg ggtgcacagg ctttaactca tcttgataca
     9061 cttgccgcaa taaaccagcg gtaatacgct cattgcctaa agctagcgca cggagctgag
     9121 cgagtacaaa aagttttact acaatgtcca tcactccttg gcttagctca taccacacat
     9181 cacggacctc atccgataac agcgcatctt tgcgttgtaa aagctgtaat tgccaaagat
     9241 tatccgtaaa agcgatccac tcttgattgg gctttccacg ttgcgtttgt tgtataggat
     9301 cccagaatat agctccaaac cctgcccctc tacgtgcaga ccgcaaatca gcctcaaaaa
     9361 tctctcgtgc tttaggggta ccaatcaaca tcactggtac gccaataata ttcaccatcg
     9421 tcacaaaaaa gttcagcatc tcttgagatc cacccgaacg agagcggctt aaatgctgaa
     9481 tttcatcaat aaccaacaac cctaaagcat gtgcattggc tatttgcgac atcaaagcca
     9541 acatggtttc tataccatga cgttttaagc cataacgacg ctcatagttc gagcccaagg
     9601 ctcgatccaa cgctctgaaa aaattcaagc agatttcttt tagcgaacca ttatgcgagc
     9661 agtctatttt caaatacacc acctgctcta cattgagttc acgatggtaa atcacctgag
     9721 gatacgtggc tagaatacga tgaagagagg tcgtcttccc actaccagaa caaccaatta
     9781 acaataagct ttgtgccgta gatcgtgcct cctcaaagcg aaatgtctcc aactctcccg
     9841 tttgaacacg ctcataacca ttttgtaaat gcttttgtaa atctcctgtt ttaggatttc
     9901 tgcctacgta gccacctcga atcatgaccg aaatacgctc acttagtagc aaatgcgtac
     9961 ctaatggctg aaaatagtca tctggaatac gacaaatggt atgagctctg ataacacggg
    10021 acttttgcaa gtcagaggaa gtaagctgta aagaggattt cagtgatgca gcactattca
    10081 ctgactcttg taatggtggt aaggcctcga taaaaggatt atcacgataa gcctctaccc
    10141 ccgtatcacg ataaactgct tgaatccggg tagcactcat gactcatcct tttctggtgg
    10201 atcctgaaat aattcaggca cgtatgtggg taggctgtaa tcttcttgat catccgcttc
    10261 cactgcgttg aaaggaataa ctttagcctc atcacctgaa gagcttggct tcaaatgctc
    10321 cgcacgtttt ttacgctccg aggtcacggc ttctttttta ttagttttaa tctgcttaat
    10381 gcgtgttgat ttgggctcag tagtactggg cgttaactta ttcgctttct gaatggtttg
    10441 ctgaataaac gcctcaagct ccctgcgttt agttaactca tcctgcttcg cattggcttt
    10501 attgtgtttt tcttgtgctt gtatatccca aacctcccaa aatgagagac ctttaaactg
    10561 ccgactacgt tccgtcagat tacagcgcca aaatacacgg ctgccaactt gcggaaacaa
    10621 ataaatcgta tcaaccagca ctgggtcata agccgcttct aaatgttgag gtctagctat
    10681 atcagtgctc cgctgcaacc aaccctcacg cagaatctct gaccccgagt aatacaaacc
    10741 ccacaaatta acgccaaatg aagaaataga gacctttcgg cgaggcagta acgctactcg
    10801 caactgctct tgctccacag cccttaaact acctgtacga tgctgcatac cccattgcca
    10861 tagctggaca ggaatagacg gtaaatctgt aggaaaatca gcatctcgat cgtatttatc
    10921 catcaccaga tggttatttc tgaataagat cgtacgcaaa ataatttgtg tgaactcaaa
    10981 taccgacaga gatgcatcta acctatagtc tgtttcacca tggcttttga tccgactgcc
    11041 ctctacaatg ccaggtgcaa aggacttaaa ctcggcttgt agtgttctaa aagtgctttc
    11101 cactatgcct ttagcatcgc cacgtctagg tggagcactt tccactcgca cattaaaact
    11161 agaaactaag gcttcgacct gatgactcat taattcgcca cggtccgcta gcaacacatc
    11221 tggcaaacct acacacggcc agtctgagct actaatctca atatcatgct gggcacaaat
    11281 ggccgttttg tcagagcaag catttacaaa agcctgcatc gccaccacat aagacggatt
    11341 ttcaaagccg atataaaagc ccgtgatcat ccgactaaac acatcaatca caatgtaaag
    11401 cgttggtctt cctatgattt tttggcgatc atgatgatcc actaaataaa tatcagcaat
    11461 cgtggcatca atctcataac gactcccagg gcctaacgcc tgagaagtgg ctgtactact
    11521 taagggtcgt acgtcttttt tatatacccc tgctttaact ctagacttta agcgctgagc
    11581 tttagggtat tctcgatcat aaaaataacg aaactgacgt agtgttgggt aatcctcttg
    11641 gggaatgcga ggaaaatact gagcaaacaa gtccacaaat cgtctatagg caacggtggt
    11701 ctttgtacct ttttgattta acaggtgctt ttctatggtc aacctaaaaa ggcgttcaat
    11761 ctcgggcgtt accttggttc cttcaccctt accatattct ctggctcggc caatctttgc
    11821 tgttcctgtc gctgaacgtc tttcccctgg tgcaccgctg tttttgtagt caggaattaa
    11881 tgcattaggc gtttgaccac gctgccagta acggcgtaac aacttataaa ctgtagcctt
    11941 agtaacctta tgttcttgga ccacatgctc aacgagttcg cttctgactt tagggtcgaa
    12001 acgatcctta ctattaataa taggaagaat ttttcgataa tcctcctcgc gcttctggaa
    12061 gctgacagaa tctacagacg gctcttctaa atcaagatgt acataagggt catcagttct
    12121 aactaagcgg ccttcatcta aatactgcat tagcaacaac tcagccctag cttgtggaac
    12181 tcctttatca gcgcttattt gcatccagac aacttggcca tcctctatag ccaaaatgcg
    12241 atacggatca ttatcaaata gcacaacctc attaatttgc cacatagcgc aactcctcca
    12301 tattcactac ttggctaata cagagatctg cacacttatt tgccctgaaa gacttataaa
    12361 tattgaactt aataaaacca tttgcggtta aggctcgtat ctcactcaat gttttgccta
    12421 actccaaatc ataagcaata tcaacctgct tacagacatt gataatgttt tcatctcctt
    12481 tttcttgcag gatatgggcc aatggggata gttgtgctaa aagctccgca gaaacttctt
    12541 ctgttttcac tgaataaagc cattcaatat tttcttttac tacgggattt atttctttat
    12601 cagtaaaaat gaaccaagga atttgctttt gctgccaata gcgacgctct agttctagtt
    12661 tttctaaggt acgctcgtct tgtaaggctg ctgcaggttt gacttgaata gcaaactgct
    12721 caaaaggacc atctttgcag tccactaaaa aatcagtaga cataacctga tctacaccac
    12781 gaataacagg atgcttaata ccactatcta ttgcaatctg cctggtatca ctaggtaata
    12841 aggggaactg ctcgcgtata tctagcacgc tgctctccca ctcaagactg agaaaaacag
    12901 caagctctaa gtcagatagc aaatgatgga ctcgtcccgt cttatgagaa taaatacggt
    12961 gggaacgacc tgaagaagga acttcttgta ctgttagcca tggaatatag tctttaccat
    13021 gcccttggcc acgcccctct ttaatacgac gggcaatttg cacttcagaa aatgaagagt
    13081 ttgctttagc cataacaaaa gtccagtatg ctttttcaca gcataactgg actgatttca
    13141 gtttacaact attctgtcta gtttaagact ttattgtcat agtttagatc tattttgttc
    13201 agtttaagac tttattgtcc gcccacaagc ccttttttat ctatgatagt gattactcaa
    13261 ccgtaaccga ttttgccaag ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga
    13321 catggtaagc cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca
    13381 catgcggcat ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg
    13441 cgaagacata caactgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt
    13501 ccaacagttc gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca
    13561 gcgggccgtg tttcagctcg ccagcagcgt aggcttcagc gtgaatgtag gagatctctt
    13621 tcagcttcaa tgcgccttcc agtgcgattg ggtactgatc gccacggccc aggaacagtg
    13681 cgtgatgttt gtcagagaaa tcttctgcca gagcttcaat gcgtttgtcc tgagatagca
    13741 tctgctcaat acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg
    13801 catccagacc tttcaggcga gacagcttcg ccaccagcat caacagctca gttaactgag
    13861 tggtgaatgc tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca
    13921 gatcggattc gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt
    13981 aacccagctc tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg
    14041 acaaggtgat catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg
    14101 cgatttcgac gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac
    14161 cggagttata agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt
    14221 cgtcggcgtt cggtcccagc tcgcttaaat caacctgacc gtggccgatg cgcccggtaa
    14281 gggtgttttt gatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa
    14341 tacctttatc gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg
    14401 cgccagtttt atcgaagatg tttaccgaac ggcgagtaat ttccgcaata tcgccctctt
    14461 caaggaagat aaagcgacgg gtcaccggca gcagcgccag ctggtcagaa gcgataaagt
    14521 tttcgcccat acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat
    14581 ccgggtgacg ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac
    14641 gcagaacagc ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa
    14701 tcacttcggt atcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca
    14761 gcggttcatg gttttcgatg atgccgttat gcaccaccat aatgtgttca gaaacatgcg
    14821 gatgcgcgtt agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg
    14881 tgccgccatg cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc
    14941 gacgcaggcg ggtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc
    15001 cgcggtattc cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga
    15061 tcgcgccaac aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct
    15121 gtatgcccgt tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta
    15181 ctgcttgtgg gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca
    15241 gaattacttt ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct
    15301 gatagctaat gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc
    15361 gcctttgcct actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc
    15421 gccgataatg gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc
    15481 gatgttaacg ttatcgccaa tttccgcatc gcccaggtaa gtcagatgac cagctttcga
    15541 gcctttaccc agacgcgctt ttttcatctc aacaaagtta ccgacgtgtg caccttccag
    15601 caactcagca ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt
    15661 cacgtcttcc acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt
    15721 aatcacgcaa ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat
    15781 aacgttagta tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg
    15841 cgccggatcg cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta
    15901 aacgcgctcc agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg
    15961 ttgcggatga acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt
    16021 gatgtagtat tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatatc
    16081 tgcgccgtta gcgatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt
    16141 ggcatctttg tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata
    16201 accggtcgga tcatccagtt tcaccgtcag cagaccaatg ccaccctgcg gtttagcatc
    16261 acgcaggcgc tggagtgttt cgacagagat cagcggcacg tcgccgtaga acattaaaat
    16321 gtcttcatca tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg
    16381 ctctgcctga agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc
    16441 gccgtgaccg tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac
    16501 atgctgaacc atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat
    16561 gcgcgtgcct ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt
    16621 cctgactgta atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt
    16681 catcgtgaaa tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt
    16741 gccattttcg accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt
    16801 aagcgtcagg tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg
    16861 tgttttatat cagcaggatc tatgtgaacg ctcttcagga cggatcacgc gtaaaaaaaa
    16921 ccagcctgtt tccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg
    16981 tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt
    17041 aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg
    17101 ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc
    17161 cagactgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt
    17221 tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga
    17281 tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt
    17341 gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag
    17401 gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc
    17461 ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc
    17521 tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg
    17581 ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag
    17641 ttcatccata cccaggatgg cgatgatgtc tttcagttcc tgataacgtt gcaggatgga
    17701 ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg
    17761 gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct
    17821 cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc
    17881 atccgcaggt acgtataccg cctgtacgga ggtgatagaa ccagttttgg tggaggtgat
    17941 acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg
    18001 catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc
    18061 aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt
    18121 cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac
    18181 tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct cacgagtacg
    18241 ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag
    18301 ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc
    18361 cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga
    18421 gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc
    18481 gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc
    18541 tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag
    18601 accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac
    18661 ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac
    18721 ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt
    18781 agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc
    18841 tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct
    18901 ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc
    18961 tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc
    19021 agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc
    19081 agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac
    19141 atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag
    19201 gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg
    19261 ccggtgacct gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag
    19321 ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc
    19381 agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc
    19441 aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta
    19501 ccatgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc
    19561 atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc
    19621 ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc
    19681 ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg
    19741 ttgctttgaa ggaatcgagg atgcctttca gtttgccttc gatttcgtcg ttgtagccac
    19801 cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag
    19861 cggcttcgaa gctgccaatt ttcgacagtt caacatccgc caggtaacca cgttctgctg
    19921 cgaacagaac cagagactgc tgcgcaacgg acatcggcgc atactgtttc tgtttcagca
    19981 gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg
    20041 caaactgaga gaacgctgct agttcacgat actgtgccag agcggtacgg ataccaccgg
    20101 acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg
    20161 ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat
    20221 cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc tgagtttcga
    20281 taatcggcag tgcggtcaga gaaccggttt tccctttcac ttcacctttg gtgaaggctt
    20341 caacgtattc ggcgttaaca cgtgcagcac gctccagcag acgagagtgg aggtagaaaa
    20401 cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag cagcagggag atctgacggt
    20461 aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac
    20521 ggaagtattc gcccattgcg caaccggcat acggtgccag gtattgcagt gcagcggatt
    20581 cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac
    20641 gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac
    20701 cggaatcacg ctggttgatg atggcatcga tagccagtgc ggttttacct gtctgacggt
    20761 caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca acggctttat
    20821 aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt
    20881 ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag
    20941 tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac
    21001 acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc gcacctacag
    21061 agtcgcgctc gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac
    21121 cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag
    21181 taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt
    21241 cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg
    21301 caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga
    21361 taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac
    21421 gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa
    21481 tgacgtctac ctcagcggta gcctcactca cggcacgaag gtgaataaac tgctccagaa
    21541 catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg ttctgaccgt
    21601 tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt tctggcgcaa
    21661 gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca
    21721 gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg
    21781 cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc
    21841 gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt
    21901 ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt
    21961 acgctcggct tcaatttctg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc
    22021 ctcagctttc gcttcgtcca gaatctgcga gcggcgtttg ttcgcctgct cgatgattac
    22081 ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag
    22141 gtccttatat gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc
    22201 gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa
    22261 cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt
    22321 taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca
    22381 cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa
    22441 agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaagaatt
    22501 tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag
    22561 cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc
    22621 agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg
    22681 atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc
    22741 cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa
    22801 atcagctcac cggcatacat gttaccgaac agtcgcaaac cgagtgaaac tggtttggac
    22861 agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc
    22921 tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgct gtagaacaga
    22981 atcaggataa atacgcccaa tgccatagac agcgttacgt tcacgtccgc agacggaacc
    23041 acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcag gtcgataggc
    23101 agtaaatcca tcaggttcat caggaatacc cagacgaaga tcgtcagggc cagcggagca
    23161 atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc
    23221 agctcaatcg cggtctgaaa cttacctggc acaccgctgg tcgccttttt ggctacgcta
    23281 cggaataaaa ccaggaacaa cagacccagc accaccgaga agaacatgga gtcaatattg
    23341 attgtccaga aggtggctgg ggggttttgt ggatccacca gcgagaatgt acgcaggtcc
    23401 agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc
    23461 atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca
    23521 ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca
    23581 ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat gtccaggcca
    23641 cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt
    23701 taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga
    23761 ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag
    23821 caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg
    23881 tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagaggtc
    23941 aaattatacg gcgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt
    24001 ttaaatattt ttccagtgca tacaattgcg acttttctgc taaccctgtt cgatcatgaa
    24061 aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca
    24121 tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt
    24181 tcactctaaa aacagatact gttttaataa atgacattta cacaacaaaa accacccatt
    24241 gacattttta ataatgtttt agcagccaat gatggttctt agcgccgatt tttagcagac
    24301 tgatattttc actaatgact tattttctgc ttaccaaaaa aagccacgtt atcttgttga
    24361 tgcaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa acctggttgt
    24421 tgttaacagt ataaccggtc aattttttat gatttttttg ataaaaatta aattttattt
    24481 gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag tttaaccact
    24541 gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcct ttgccctttc
    24601 agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac catatcgttc
    24661 agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgaagg aaactcttct
    24721 accctgctct gtactggttc aatattctcc agtttaagct catgttgcac ctgacgaagg
    24781 aaacgcacgc gtttaccaag gctatccaac agagtgaaat gggcttcagg acgcacgata
    24841 gagagtggaa tgcctggcag tcctggtccg gtgccgacat cgataaaccg ttcaccttgc
    24901 agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc attaggatcg
    24961 cggaccgaag tcaggttgta cgctttgttc catttatgca gcatattcac gtaggcaata
    25021 agctggtttt tctggtgatc ggtaagcgaa atacctgcgt ctttcagcag taaggagagt
    25081 ttgttgagca cggtgattac ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag
    25141 acatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag ccacaccagc
    25201 agaatggaga tggccgcagg cgtgacgcca gaaatacgcg aagcttggcc gatagaggct
    25261 ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac ctggcggtaa
    25321 tccagtgtcg cgggtagcag ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc
    25381 tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg
    25441 tctgtcaacg caggggcaaa cggcgtcagc gtggttaatt tttcataagt catttccgga
    25501 cgacgcagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag gtgagcattc
    25561 acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg ctgacgctca
    25621 cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc
    25681 agttcacgac cgatttcagt caaacgcaga tccgcattat cttcgcgtag catcagacga
    25741 tattctgcgc gcgaagtaaa catacgatac ggttctttgg ttcctaaagt gcacaggtca
    25801 tcaactagta cgccgagata cgcctgagaa cgtgccggag cccaaccttc tttgtcagca
    25861 gacagacggg cagcgttaag accggccagc aaaccttgcg cagcggcttc ttcgtaaccg
    25921 gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct ctccagcgtc
    25981 ggtttcaggt cgcgaggatc gaagaagtca tactcaatgg cataacccgg acgcacgatc
    26041 ttcgcgtttt ccatcccctg catagagcgg acgatttgca tctgcacatc gaacggcagg
    26101 ctggtggaga taccgttcgg ataaatttca ttagaggtca gtccttccgg ttcaaggaag
    26161 atctgatgct gatttctgtc ggcgaagcgc atgactttgt cttcgatcga cgggcagtag
    26221 cgtgggccga caccttcgat cacccctgca tacattgggc tacgatcgag gttactgcgg
    26281 atcacatcat gggttttctc gttggtatga gtgatataac acggcacctg ctggggatgc
    26341 tgggacgcat tgcccataaa cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc
    26401 agcacactaa agtcgatagt tcgagcatca atacgcggtg gtgtcccggt tttcagacga
    26461 ccaacgcgca gcggcagttc acgcaaacgg tgagaaagcg gaatggacgg cggatcacca
    26521 gcacggccac cgctgtaatt atccagaccg atatgaattt taccgtcgag gaacgtccca
    26581 acggtgagca cgacggcttt ggcacggaac ttcagtccca tttgggtaac agcaccgacc
    26641 acgcgatcgt tttcgacaat aagatcttca accgcctgct ggaagatcat caggttcggt
    26701 tggttctcca gcgccgtacg taccgcctga cggtagagca cacgatccgc ctgagctcgg
    26761 gtagcgcgaa ccgccggtcc tttgcttgcg tttagtatcc taaactggat acccgcttga
    26821 tcgatcgctt tcgccatcag accgtcgagt gcatccactt cttttaccag atgtcccttc
    26881 ccaataccgc cgatcgccgg gttgcagctc atctgtccca gagtgtcgat attgtgtgtc
    26941 aaaagcagag tctgttgacc catacgcgcc gcggccatcg cggcctcggt gcctgcatga
    27001 cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat tgcctcgcat
    27061 aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact ttgtcggctt
    27121 gagaaagacc tgggatcctg ggtattgaaa agaagatcta tttatttaga gatctgttct
    27181 attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg gcttttaaga
    27241 tcaacaacct tgaaaggatc attaactgtg aatgatcggt gatcctggac cgtataagct
    27301 gggatcagaa tgaggggtta tacacaactc aaaaactgaa caaccgttgt tccttggata
    27361 actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca cgatctgtat
    27421 acttatttga gtaaattaac ccacgatccc agccattctt ctgccggatc ttccggaatg
    27481 tcgtgatcaa gaatgttgat cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg
    27541 agttctgcct cgagtttatc gatagcccca caaaaggtgt catattcacg actaccaata
    27601 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    27661 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aatcagccag
    27721 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    27781 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    27841 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    27901 cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg
    27961 gcttgatagt acgcatgatc gggttttgca ggacgatcaa tgtctcggtg gactgaattt
    28021 catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc gaacggcaca
    28081 ttacttttat aaagatgctg tagtggcctg ttgtgtaata ggcttcagtg acttcatcaa
    28141 ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat ataatgccga
    28201 taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc gccccggtaa
    28261 tgatccccgc ctgcttcatt ttctctaccc gaacgtgaat cgtccccgga ctgacgccaa
    28321 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    28381 cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca gattgatgat
    28441 tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggctt tttattgaat
    28501 gattattgca tatgtgtcgg tttttattgc ttaatcataa gcaacaggac gcaggagtat
    28561 aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg aaatctcact
    28621 tttctcgtca actggaagaa cgtctggggc tgatcgaagt ccaggcaccg attcttagcc
    28681 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    28741 tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag tggaaacgtc
    28801 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    28861 ttcgccccga tgaagaccgt ctttctccgt tgcactcggt ctatgtagac cagtgggact
    28921 gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc acggtagagg
    28981 cgatctgggc gggaattaaa gcaaccgaag ctgcggttag cgaagagttt ggcctggcac
    29041 cgttcctgcc ggatcagatc cacttcgtac acagccagga gttactgtct cgttatccgg
    29101 atctcgatgc caaagggcgt gagcgggcga tagcgaaaga tcttggcgcg gtattccttg
    29161 tcgggattgg cggcaagctg agcgatggtc atcgccacga cgtgcgcgca ccggattatg
    29221 atgactggag caccccgtca gagctgggtc atgcgggtct gaacggcgat attctggtgt
    29281 ggaaccctgt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    29341 acacgctgaa gcatcagctg gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc
    29401 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc ggccagtctc
    29461 gtttgactat gctgctgctg caactgccgc atatcggcca ggttcagtgt ggagtatggc
    29521 cagctgctgt tcgcgagagc gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    29581 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    29641 gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    29701 cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat
    29761 caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    29821 acaactggca agatcggtgc cgccacgaaa cagctggctt aaaaaacgga ttgcttgttc
    29881 gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata gcataatata
    29941 gcagcgccgg ttttctgcga gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca
    30001 ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa acggcccgcg
    30061 cggctgttca tcgtaatctt tatgtaccac cggacgttcg atcacttttt cacgccacga
    30121 ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    30181 ctccagttcc gttatcccta gtgtcgccag ttctggcggc aggagacgta aaatatcatc
    30241 gctttgttgc agaccatcaa cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg
    30301 gaaggtttcc atctgcgcat cgttgcgcgg tattgatttg gcttcccgag aacgccccag
    30361 ctgctctgcc aggcgtttca gttccggctt ttcgttaaga aattcaccgt atttcacaat
    30421 caactgatag tcgccacgtt taagctggcc ggcgctcata tcccacagac gaccagctgc
    30481 ggtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac
    30541 ttcacttaac agttgttcgc gttcttcttc taatagctgt tgatttaacg tcgttgcttg
    30601 cacgatcaga cttaaacgcc agcgctggag aaaaagcgtg tgtaacgctg aagttattgt
    30661 gctgttagca tcaaccaact gccgggcttg ttctgcccat ggggaattca gacgatgcag
    30721 taagtccagg atctgtggta gctgcacaat aaactgcggc gtggagagga gctggctttg
    30781 ctgatagcac atcacctctt cggtgagttc tggcgggact cgggcatctt tcagccgact
    30841 gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gctgccttca gtcgtgggaa
    30901 tttttcaaag aagaccgcca gctgcggtga ggccagcagt gcgatgatca tctcttcgat
    30961 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    31021 gcctggcgaa tctgtgcacc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    31081 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg
    31141 ctttcaagct cttccagttg ttgttttatt tcacctggca gaccttcagc cgatgaaccg
    31201 ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgta caacaagatg ttgggtgcta
    31261 tcaacttcca gattcagttt ctgggcaaag ccgatacagt tcagtttgcc gcgaatttca
    31321 ccacctttac tcagccactg ttccagcgcg ctacgctcaa aggagatatg aaccacttcc
    31381 atatcatgca gttttaacgg tttttgcagc agcagagtca gagtggaagc agtaacgtta
    31441 acagggagtt gatactgctg acgacggcta aaaatgccgc ccagacgaat taccgttaag
    31501 gctgttttat cgctttgttg ctgctgaagt tgcaggtgac gttgcacaat cgcgcccagg
    31561 cgggtcaaca tcccttgctg ttgccaggcg tgaccggtca tcaatacatc aatttgttgt
    31621 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatct
    31681 accggggcaa cagcactgcg accgctaaaa aaggcgctgg cctgcaataa tcggatcgct
    31741 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    31801 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    31861 cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt gtcgttttca
    31921 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cctgcacttt atctaaccac
    31981 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    32041 ttggaggccg ccaccagcag acgcatcggg attttttcta cgtgtgcacc gttgcggaac
    32101 tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc tttccagatc
    32161 tcatccagaa agacaatttc tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    32221 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    32281 cgggtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    32341 atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc acttaatgcc
    32401 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    32461 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    32521 ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    32581 tctcaaaggg ggg
//
LOCUS       DAJTMK010000026        32285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTMK010000026 DAJTMK010000000
VERSION     DAJTMK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32285
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.026"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            197..991
                     /gene="panB"
                     /locus_tag="OO135_001054"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="OO135_001054"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5396987.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="OO135_001055"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="OO135_001055"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5396988.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OO135_001056"
     CDS             complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OO135_001056"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5396989.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="OO135_001057"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="OO135_001057"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5396990.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4899)
                     /locus_tag="OO135_001058"
     CDS             complement(3700..4899)
                     /locus_tag="OO135_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013991358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5396991.1"
                     /translation="MLFTASVSAALPARYMQTIENAAIWAQIGDKMVTVGNIRAGQII
                     AVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSNQNLITWKDTP
                     VYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYISALDAQPDNGL
                     PVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMVQLEGYVKNKIN
                     LPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKWDPKSLQFMSV
                     SELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRALAQFNPHVWY
                     LSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILRTDSLETMSRL
                     VSNQPQG"
     gene            complement(4994..5434)
                     /locus_tag="OO135_001059"
     CDS             complement(4994..5434)
                     /locus_tag="OO135_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5396992.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLLLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5538..6308)
                     /locus_tag="OO135_001060"
     CDS             complement(5538..6308)
                     /locus_tag="OO135_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5396993.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6305..7231)
                     /locus_tag="OO135_001061"
     CDS             complement(6305..7231)
                     /locus_tag="OO135_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5396994.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7340..8002
                     /gene="can"
                     /locus_tag="OO135_001062"
     CDS             7340..8002
                     /gene="can"
                     /locus_tag="OO135_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5396995.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVIQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8043..8579)
                     /gene="hpt"
                     /locus_tag="OO135_001063"
     CDS             complement(8043..8579)
                     /gene="hpt"
                     /locus_tag="OO135_001063"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5396996.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFVADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8785..11175
                     /gene="gcd"
                     /locus_tag="OO135_001064"
                     /pseudo
     CDS             8785..11175
                     /gene="gcd"
                     /locus_tag="OO135_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
     gene            complement(11222..12772)
                     /gene="cueO"
                     /locus_tag="OO135_001065"
     CDS             complement(11222..12772)
                     /gene="cueO"
                     /locus_tag="OO135_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5396997.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12938..13285
                     /locus_tag="OO135_001066"
     CDS             12938..13285
                     /locus_tag="OO135_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5396998.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13391..14257
                     /gene="speE"
                     /locus_tag="OO135_001067"
     CDS             13391..14257
                     /gene="speE"
                     /locus_tag="OO135_001067"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5396999.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14273..15067
                     /gene="speD"
                     /locus_tag="OO135_001068"
     CDS             14273..15067
                     /gene="speD"
                     /locus_tag="OO135_001068"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5397000.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15104..15466)
                     /locus_tag="OO135_001069"
     CDS             complement(15104..15466)
                     /locus_tag="OO135_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5397001.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15641..18238)
                     /gene="acnB"
                     /locus_tag="OO135_001070"
     CDS             complement(15641..18238)
                     /gene="acnB"
                     /locus_tag="OO135_001070"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5397002.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18593..20243
                     /locus_tag="OO135_001071"
                     /pseudo
     CDS             18593..20243
                     /locus_tag="OO135_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20314..21738)
                     /gene="lpdA"
                     /locus_tag="OO135_001072"
     CDS             complement(20314..21738)
                     /gene="lpdA"
                     /locus_tag="OO135_001072"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5397003.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22063..23955)
                     /gene="aceF"
                     /locus_tag="OO135_001073"
     CDS             complement(22063..23955)
                     /gene="aceF"
                     /locus_tag="OO135_001073"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5397004.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPLSLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(23970..26633)
                     /gene="aceE"
                     /locus_tag="OO135_001074"
     CDS             complement(23970..26633)
                     /gene="aceE"
                     /locus_tag="OO135_001074"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5397005.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDDLLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26794..27558)
                     /gene="pdhR"
                     /locus_tag="OO135_001075"
     CDS             complement(26794..27558)
                     /gene="pdhR"
                     /locus_tag="OO135_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5397006.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERICELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28099..29472
                     /gene="aroP"
                     /locus_tag="OO135_001076"
     CDS             28099..29472
                     /gene="aroP"
                     /locus_tag="OO135_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5397007.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29515..30369)
                     /gene="ampE"
                     /locus_tag="OO135_001077"
     CDS             complement(29515..30369)
                     /gene="ampE"
                     /locus_tag="OO135_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5397008.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30366..30917)
                     /gene="ampD"
                     /locus_tag="OO135_001078"
     CDS             complement(30366..30917)
                     /gene="ampD"
                     /locus_tag="OO135_001078"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5397009.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31005..31898
                     /gene="nadC"
                     /locus_tag="OO135_001079"
     CDS             31005..31898
                     /gene="nadC"
                     /locus_tag="OO135_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5397010.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32101..>32285
                     /locus_tag="OO135_001080"
     CDS             32101..>32285
                     /locus_tag="OO135_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5397011.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGG"
BASE COUNT         7785 a         8769 c         7939 g         7792 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccaag taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactactcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gctttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctca gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccaaa
     4561 tggcgcactg cccgcactcg gcgcgttata gaccggcgta tctttccagg taattaagtt
     4621 ctgattactc agcggcttgt tgaggtcacc caaaccgtct tcaacttttt gtcgcccctg
     4681 aactggctcg agatgccctt tatcgataaa ccctttacca aagccaaaat taaatgcgta
     4741 ataacttgcg gcagtgggct ccacggcgat gatttgcccg gcccgaatat tccccacggt
     4801 caccatcttg tcgccaattt gcgcccagat cgcagcattt tcgatggttt gcatataacg
     4861 ggcaggtaac gcggcactaa cgcttgcggt aaacagcatc agcaggatga ataacagctt
     4921 gtttatacat gaaaaaataa ccgagggtga aacaacgctt gtattgtagc aaaagcgcca
     4981 tcataatcgc gggttaatac tcatacaaat tatgtttgag gacaaaaggc ggattttttt
     5041 gttgttggtg ccaaagacta ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa
     5101 actctgaact ggtcatggtt tcacggcaag ccatcatctg ttcaattaac agtaacgtga
     5161 caccagaaat cacttcacaa cgggagtgtt tatggcttaa taatgaagcc acgcggtacg
     5221 gtggcgctcc ggctatatcg gttaagaaga tgacaccctc accagagtcc gcttcatgca
     5281 gagcatcgca catcatgcgg ctgagcatat tagagcttaa tccgcgccag aaattcacgg
     5341 cccggcactg aagaagtgcc ccatgttttt tctccagcgc atccagtatc tcttgcgccc
     5401 ggtcatcgtg acaggtaatt acccaaccta acatcgcctt cctccttagc cgaaatcagt
     5461 ctatcctgac cattgttgat aaagggtgat agccgtcaaa actcctctcc cccgaatcca
     5521 gaggagagga aatagcctta gctacgcaaa ccacgtccac gttggatcag cgaccaacag
     5581 atcaaataaa acaccacaat aaagaccacc agtacgccaa aggtagtggc cagcggcaca
     5641 tcatttatac cgaggaagcc gtagcggaaa ccactgatca tataaacgat tgggttcagg
     5701 tgcgacagcc cttgccagaa cggcggcaac aaagtcaggg agtaaaacac cccgcccaaa
     5761 tacgtgagtg gcgttaacac aaaggttggc accaggctga tgtcatcgaa cgttttggca
     5821 aacacgccgt tcagcaaacc cgcaagggag aatagcaccg ccgtgagcac tagggttaag
     5881 gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat tgccgtcacc
     5941 agaatgccaa caaacagacc acgcgccacg ccaccgccga catatccggc aataatgacg
     6001 tgagtcggaa ccggcgctac cagcagctct tcaatattac gctggaactt ggcaccaaaa
     6061 aatgatgacg cgacgttagc gtaggcattg gtgatcaccg acatcatgat cagccccggt
     6121 acgatgaact gcatatagct gaagccatgc atatcgccaa tacgcgaacc tatcaggtta
     6181 ccgaagataa taaagtaaag ggtcatggtg atgactggcg gcaccagcgt ctgcacccag
     6241 atacgcataa agcgatggat ctctttcgcc cagatgcttt ttagcgccac ccagtaaaga
     6301 tgcatcatgc gcgatctcct tgtttttcat taaccagtga aacaaacagc tcttccagac
     6361 ggttagcttt gttacgcata cttaatacct gaatgccctg ctcacttaac tgcgtaaata
     6421 cgctgttgat cccctgctca cgcagcactt caacttccag cgtcgcggta tcgaccagtc
     6481 gatactgata gccatcgagc ttcggtaacg ggcttttcgg tgcgagatcg agaataaagg
     6541 tttccgattt cagcttcgcc agcagcgcct tcatcgaggt attttccacc agctcaccgt
     6601 gttgaataat gccgatattg cggcacagca tttctgcttc ttccaggtag tgtgtagtga
     6661 gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt gagcggcgaa
     6721 gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt tcatgcatta
     6781 acgcacgggc aatcattaaa cggcgcttca tcccgccaga taacatacgc gcacgttcgt
     6841 tgcgttttcc ccataaatcg agttgtttga gatatttttc gctgcggatg tacgcttctt
     6901 tgcgctccac gccgtagtac cctgcctgat tcaccacaat ttgctgcacg gtttcaaacg
     6961 ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg tttagcgttc acgacatcct
     7021 tctcgagatc gtaaccaaat acgctgaccc gcccggaggt tttatttacc agagagctga
     7081 taataccgat agtggtcgat ttcccggccc cgttcggccc gagaagcgca taaaaatcac
     7141 ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac gccgcctgga taggtttttt
     7201 taagctgttg aagttccagt gcaatggtca taaattttta cttaccttac gttcttacac
     7261 tttatctgtg gtttaaatcg tcccggagtt gccctatatt agccaaacgt aattatttgg
     7321 ttacaggtcg ttaacctcca tgaaagacat agatacactc atcagcaaca atgcactatg
     7381 gtcaaaaatg ctggtggaag aggatcccgg gttttttgag aaactggcac aagcgcaaaa
     7441 accgcacttt ctatggattg gatgttccga cagtcgcgtt cctgcagaac gtttaaccgg
     7501 tcttgagccg ggcgaactct ttgttcaccg taatgttgct aacctggtca ttcacaccga
     7561 cctgaactgc ctttccgtga ttcagtatgc agtggatgta ctagaagttg aacacattat
     7621 tatctgtggc cactacggtt gcggcggcgt acaagccgca gttgaaaacc cggaactggg
     7681 gcttatcaac aactggctgc tgcatatccg cgatatctgg ttcaaacata gctcattgct
     7741 cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt gaactgaacg tcatggaaca
     7801 ggtgtataac ctaggccact ccaccattat gcaatcagcg tggaaacgcg ggcagaaagt
     7861 taccattcac ggctgggcct atggcattca cgacggcttg ctgcgtgatc tggatgttac
     7921 tgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca acctcaagct
     7981 gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc aacacatccg gcaacttcac
     8041 acttactcgt ccagcagaat cactttgccg atatacggca gatgacggta acgctgtgcg
     8101 taatcaatgc cgtaacccac cacaaactca tccgggatcg agaaaccgat aaattctacc
     8161 gggacgttca cttcacgacg ggacggttta tccagcagcg tacaaatcgc cagcgacttc
     8221 ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg tattccccga gtcgatgata
     8281 tcttcaacaa tcagcacgtc cttgccacgg atatcttcat ccagatcttt gaggattttc
     8341 acatcacggg tggtggacat gccgctaccg tagctggagg cggtcataaa gtcgacttca
     8401 tgagatacct gaacttcacg gcacaggtcc gccacaaaca taaatgagcc acgcagcagc
     8461 cccaccagca ccatatcgct gccgctgtct ttgtaacgct cagtaatctg acgacccagt
     8521 tcggcgatac gcgctttaat ctccgcttcg gggatcatta cttctacagt atgtttcata
     8581 tctctaacca tatgattaaa aaacaaatca cttcgcacgg gcaggttttg gcaccgcaac
     8641 aaagcgcaag acacgcagta taccagtaaa actattcatt cgcggaatct gttaataaag
     8701 ctcgaattgt gatgacgatc acacatgtta aaacctataa ttaattgcta ctaaaatatt
     8761 aatgaattga aatggtgtct ctttatggca attaacaata caggctcgcg acgattactc
     8821 gtcacgctaa cagtcctttt tgcagcgctt tgcgggctgt atctactcat tggcggaggc
     8881 tggctggtcg cgattggcgg ctcctggtac taccctatcg ctggccttgt gatgctcggc
     8941 gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt ggctatacgc cgccctgctg
     9001 ctcggcacca tgatttgggg cgtctgggaa gttggtttcg acttctgggc gctgactccg
     9061 cgcagcgata ttctggtctt cttcggcatc tggctgatcc tgccgtttgt ctggcgtcgc
     9121 ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg tggcactgct gattagcggt
     9181 agtatcctga cctgggccgg atttaacgat ccgcaggaaa tcaacggcac cttaagcgcc
     9241 gatgccacac ctgcggaagc tatctcaccc gttgccgatc aggactggcc tgcctatggt
     9301 cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa ttaacgccga taacgtccat
     9361 aatctgaaag aagcctgggt gttccgtact ggcgatgtga agcagccgaa cgatccgggc
     9421 gaaatcacca atgaagtgac gccgattaaa gtgggcgaca ccctttacct gtgtaccgct
     9481 caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag agaaatggca ttacgatcct
     9541 aagctgaaaa ctaacgagtc tttccagcac gtaacctgcc gtggcgtctc ttatcatgaa
     9601 gccaaagcag aaacggcttc gccggaagtg atggcggatt gcccgcgtcg tatcattctt
     9661 ccggtcaatg atggtcgact gattgcgatt aacgctgaaa acggcaaact gtgcgaaacc
     9721 ttcgccaata aaggcgtgct caatctgcaa agcaatatgc cagacaccaa accgggtctg
     9781 tatgaaccga cttcgccacc gattatcacc gataaaacca tcgtgatggc aggttcagtt
     9841 accgataact tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga tgtcaacacc
     9901 ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc cgaacgcaat cccgtctgac
     9961 gaacacacct ttacctttaa ctcgccaaac tcctgagcac cagcggccta tgacgcgaag
    10021 ctggatctgg tctatctgcc gatgggcgtg accacgccgg atatctgggg cggtaaccgc
    10081 acaccggaac aggaacgtta tgccagctcg attctggcgc tgaatgccac taccgggaaa
    10141 ctggcgtgga gctaccagac cgttcaccac gacctgtggg acatggatct tccggcacag
    10201 ccgacgctgg cggacatcac cgttaatggt cagaaagtgc cagttattta cgctccggcg
    10261 aaaaccggca acatttttgt gctcgatcgt cgcaatggcg aactggtggt tccggccccg
    10321 gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg ttactccgac ccagccgttc
    10381 tctgaactga gcttccgtcc gaagaaagat ttgagcggtg cggatatgtg gggagccacc
    10441 atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga aggcattttc
    10501 acgccgccat ctgaacaggg cacgctggtc ttcccgggta acctgggaat gtttgaatgg
    10561 ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat ggcactgccg
    10621 tttgtttcga aactgatccc acgcggtccg ggtaacccga tggagcagcc gaaagatgcc
    10681 aaaggcacgg gtacggaatc cggcattcag ccacagtacg gtgtaccgta tggtgtcacg
    10741 ctcaacccgt tcctctcacc gtttggtctg ccgtgtaaac agccagcatg gggttatatc
    10801 tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga aacgtattgg tacgccgcag
    10861 gacagtatgc cgttcccgat gcctgtaccg gtgccgttca atatgggtat gccgatgctg
    10921 ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg ccgctacggc agataactac
    10981 ctgcgcgctt acaacatgag caacggtgaa aaactgtggc agggtcgttt accagcgggt
    11041 ggtcaggcga cgccaatgac ctatgaagtg aatggtaagc agtatgtggt gatctccgca
    11101 ggcggtcacg gttcatttgg tacgaagatg ggcgactata ttgtggctta tgccctgccg
    11161 gatgatgtga aataaccata atgcccggag agatccgggc atatttccga atacggtctt
    11221 tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga tggcagtgcg
    11281 ccatataagc atgttctttc ggtgcgtcgt gattaaactt caccagcact tcactgacat
    11341 tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc ggtttgccat
    11401 tttctgacaa gatacggaac tgcgtgccgt gaatatggaa cggatgcagc atcatgtcgc
    11461 ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac atcggcttat
    11521 tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac ttcccgccgt
    11581 ggttcatatg attcatatta ccgtgcccca tatggcccat catctggctg tgatccattc
    11641 cggccatcgc ctgatcgcca tatttctcca ttagcatctg catacccatc atatcgagca
    11701 tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa ggtaacgcag
    11761 gcaggctact taatgtgtct ggcaaagcac cggaggcact aatagcaatc ggctgaatcc
    11821 gcattaccgg atgcggcttg tcaaacggcg caatcgccat ccccatctgg ctgaccggta
    11881 gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca aaacgctcgc
    11941 ccatcagcac cggcagctcg ctcaccttca ccggttcagg tagcagacca ccgtcgctgg
    12001 caatcacata cagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg gcattacagc
    12061 cattgagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgtggg tagattgcac
    12121 cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc ggtcatcaca tccagttgat
    12181 aatcaatctg cccatcagcg ttaaattttt tatcctgaac aatcaccgga acatcatcga
    12241 taccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatt accaccagcc
    12301 cagccaaccc catcgccacc tgtcgcccag tcttgccatg ttgatgtgga tggaaccagc
    12361 aagtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct ggtggaataa
    12421 ttccctgcgg gccgccgtcg acttcacccg gtacttccag cccgtgccag tgcaacgtcg
    12481 tctcttccgt cagttggtta tagatatcaa ccgttaccgc tttgccgcgc tgtaatttca
    12541 ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc tcgccaaagg
    12601 tagactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc gtgagcaaat
    12661 cagggatcgg taacgttggg cgttccgccg caaagactgc gcggctccac agcggcaagg
    12721 ctgaagccac acccagcgcg acggaatatt ttaagaaatc acgacgttgc atagttattt
    12781 ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc cccttacggg
    12841 aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg ctcgcagtgt
    12901 gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt tcagaacagt
    12961 gttattcggc agtctgatgg ccgtttgcgc aaacagttac gcgctcagcg agtctgaagc
    13021 cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt gtggttacca
    13081 gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc aaaaccagtg
    13141 ggatctcagt aattacgaca ccttcgacat gaaagccctc ggtgaagaca gctaccgcga
    13201 tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa gccctggccc gcgattcctt
    13261 aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata ttgaccgtgc
    13321 atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt aacaaaggag
    13381 gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc agtttgggca
    13441 gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccaag atctgatcat
    13501 ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac aaaccaccga
    13561 gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg cccacggtca
    13621 cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg aagtaacccg
    13681 acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg tgtcgttctg
    13741 ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct ttaagctggt
    13801 gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg tcattatctc
    13861 cgactgcacc gatcctatcg gtcccggcga aagtctgttc acctcagcat tttatgaagg
    13921 ctgcaaacgt tgcctgaatc ctagcggtat cttcgtcgca caaaacggcg tctgcttttt
    13981 acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca gcgacgttgg
    14041 cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg catgggcgac
    14101 agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt ttctcgcttc
    14161 tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg ccttacctca
    14221 gtatctgcaa gatgcactgg cttcacagcc gtcctaagga gaagataaga gattgaaaaa
    14281 actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta tttacgatat
    14341 ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg atgaactcta
    14401 taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg gggccaatat
    14461 tcttaacatc gcccgccaag attacgaacc acagggtgcc agcgtcacta ttctggtgag
    14521 tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc cactgccaga
    14581 aacggtcgtt gcccatctcg ataaaagtca tatttgcgta catacctacc cggaaagcca
    14641 tcctgaaggt ggtttatgta ccttccgcgc cgatattgaa gtctctacct gcggcgtgat
    14701 ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata tcgtaaccat
    14761 tgattatcgc gtgcgcggtt ttacccgcga cattaacggt atgaagcact ttatcgacca
    14821 tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt atgacatggt
    14881 ggatgtaaac gtctatcagg aaaatatctt ccataccaag atgttgctta aagagttcga
    14941 ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg agcgccagga
    15001 aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca atatgccagc
    15061 tgtttaacgg ctctggcgga gctccaggct ccgccagatt tatttacttc tgctgcacga
    15121 aattgcggta agccgccacg acctgcagaa aatcctcaac gccgcatagc gacaggcttt
    15181 cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg
    15241 cgcgaaccat cacctcttca ccgtccatcc acagggtgta ttcatgccct gcccgttgcc
    15301 aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc cacttcatca agcaaggcca
    15361 ggttttcttt cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatac
    15421 gcacctttac cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg
    15481 ctcccggcaa tgcgccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg
    15541 agaaaaacag cggaacggcg atggtttaga aataaaaaat gcccgggaat ctcattcacc
    15601 gggcattgtg tcgtttatgc gcagcgcgtg cgcttactta ttacaccgca gtctggaaaa
    15661 tcaccccatc ggctttttcg gtgtactgag aaagttggtt gaagttcaga taacgataag
    15721 tatcaacggc agttttatct acttgcgcaa cgtaggtctg gtactcttcc ggcgtcggca
    15781 gtttgccaat cagcgccgca acagccgcca gttctgcaga agccaggaag acattcgcgc
    15841 cagtccccag acggttcggg aagttacggg tagaggtgga aaccaccgtc gcaccgtctg
    15901 ctacacgcgc ctggttaccc atacacagag aacagcccgg gatctcgata cgagccccgc
    15961 tcttaccgaa gacgctgtag tagccttctt cagtcaactg tgcggcgtcc atacgggttg
    16021 gcggtgccac ccacaggcgg gtcggcaact ggcctttatg cgcatccagc agtttacccg
    16081 cagcacggaa gtgaccgatg ttagtcatgc aagaaccgat aaatacttcg tcgatcttct
    16141 cgccctgtac cgcagacagc ggacgcgcgt catccgggtc gttcggagca cacaggattg
    16201 gctctttaat atccgccaga tcgatgtcaa tcactgccgc gtattccgca tctgcatcgg
    16261 cttccagcag ctcaggattc gccagccatt tttccatgcc ctgaatacga cgttccaggg
    16321 tacgacgatc gccgtaacct tccgcgatca tccacttcag caggacgata ttagagttaa
    16381 ggtattcgat gattggttct ttgttcagct tgatagtaca accagcggca gaacgctcag
    16441 cggacgcatc ggtaagctca aaggcctgct caactttcag atccggcaga ccttcaattt
    16501 ccagaatgcg gccagagaag atgtttttct tgcctttctt ctcaacggtc agcagacctt
    16561 gtttgatggc atacagcgga atggcgtgta ccagatcgcg cagggtgatg cccggctgca
    16621 ttttgccttt gaagcgcacc agaacggatt ccggcatatc cagcggcatt acgcctgttg
    16681 cggctgcaaa tgccaccaga ccagaacccg ccgggaaaga gataccgatc gggaaacggg
    16741 tatgggagtc acctccggta ccgacggtat ccggcagcag catacggttc agccaggagt
    16801 ggattacgcc gtcacccgga cgcagcgaca caccgccacg gttcataatg aagtccggca
    16861 gcgtgtggtg cgtgttcacg tcaactggct tcggatacgc cgcggtgtgg cagaaagact
    16921 gcatcaccag gtcagccgag aagcccaggc acgccaggtc tttcagttca tcacgggtca
    16981 tcgggccggt agtgtcctgt gaacctacag aagtcatttt cggttcgcag tacgcgcccg
    17041 gacgaatgcc tttcacgcca caggcacggc ctaccatttt ctgcgccagc gagaagccgc
    17101 gatcgctctc agcgacatct ttcgcctgac ggaacacatc actgtgcggc agaccaagtg
    17161 cttcacgcgc tttggtggtc aggccacgcc cgataatcag cgggatacgg ccgccagcac
    17221 gcacttcatc aatcagcacg tcggttttca gttcgaaggt cgccagcagt tcgcctgttt
    17281 cgtggttacg cacttcacct ttgtacgggt aaacgtcaat cacgtcgccc atgttcaggt
    17341 tagagacgtc gacttcgatt ggcagtgcac ccgcgtcttc catcgtgtta aagaagatgg
    17401 gtgcaatttt accgccgagg cacaaaccac cgccgcgttt gttcggcaca tgtggaatat
    17461 catcgcccat aaaccacaga acggagttcg tggcggattt acgcgatgaa cccgtaccca
    17521 caacgtcacc gacgtacgcc agcgggaaac ctttctgttg cagagcttca atctgtttga
    17581 tcgggccaac aacacctggc tggtctggct caataccttc acgggcgttt ttcagcatcg
    17641 ccagcgcgtg cagtgggata tccgggcgcg accacgcatc cggtgccgga gaaaggtcat
    17701 cggtgttggt ttcgccagtg actttgaaga cggtaacggt cagtttttca gccagcgccg
    17761 ggcgattcag aaaccattcg gcatccgccc aggactgcat tacctgcttc gcatattcgt
    17821 tgcctgcttt cgctttctct tctacgtcat agaagttatc gaacatcagc agtgtgtgag
    17881 aaagtgcttt ggcagcgatc ggtgccaatt tggcatcatc cagcgcgtcg atcagcggat
    17941 gaatgttgta accaccctgc atggtgccca gcagttcgat ggctttttcc ggagtcagca
    18001 gaggggattt ggcttcgcct ttcgcgatag cagccaggaa gcctgctttg acataggcgg
    18061 cttcatcgac gccaggggga acacggttgg ttaacagatc taacaggaat tcttcttcgc
    18121 ccgcgggcgg gtttttcagc agctctacaa gtgcggccat ttggtttgca tccaggggtt
    18181 tgggcgcaat cccctcagcg gcacgctcag ctacgtgctt acggtattct tctagcacga
    18241 cggttctcct cgctctcatt gtcatagtgc ggcaggcgat tctcttcacg ctcctgtgag
    18301 acagcagttt gtagggtaaa tgcccagtac cgcaacgggc agaatagcag gattttgacg
    18361 acgtgttaat ctgtttacaa aaaagcaaca taaaaaagtg gctgaatcgt taaggatggt
    18421 ctagagattg tttccctccg taaaaacttc gttaattcgc atggtgataa tcacctttct
    18481 caacgaatcc caagattagt caaaatttaa actaccgcct ctttatactc ggattaacag
    18541 cacctgcggg tggcagttcg ccgaccattg cgatttcctt gagatccaaa ttatgaaaat
    18601 gactttgccg tttaaacccc atgtgctggc actaatttgc agtgccgggc tttgtgccgc
    18661 ctctgccggg ctatatataa aaagccgcac agtggaagcg gctgtggaaa cgcaatcgac
    18721 acaactggct gtgtctgacg ctgccgcggt tacgcttcct gcaacggttt ccgcgcctcc
    18781 cgtaacaccc gccgtcgtta aatccgcatt cagcactgca caaatagatc aatgggtcgc
    18841 gcccgtcgcg ctgtatcccg acgccctact ttcgcaggtg ctgatggcat caacctatcc
    18901 ggcaaacgtt gctcaagcag tgcaatggtc gcacgataat ccacttaaac aaggcgatgc
    18961 tgctattcag gcggtatctg accagccgtg ggacgccagc gttaaatcac tggtggcctt
    19021 tccacaattg atggcattga tgggcgaaaa cccgcaatgg gtgcaaaacc tgggcgatgc
    19081 ttttctggcg cagccgcagg acgtgatgga ctcggtacag cgattgcggc aactggcgca
    19141 acaaaccggt tcgctgaagt catcaaacga acaaaaagtt attaccacaa cgaagaaagc
    19201 tgtaccggta aaacagacag tcactgcacc cgtcatacca tccaataccg ttttaactgc
    19261 cagccccgtc attacagagc ctgcaacaac cgtcatttcc attgagcccg ccaatcctga
    19321 tgtggtctat attcccaact acaacccaac cgtggtttac gggaactggg ccaatactgc
    19381 gtatccgccg gtttatctgc caccaccagc cggagaaccg tttattgaca gctttgtgcg
    19441 cggattcggc tatagcatgg gtgttgctac cacgtacgca ctattcagca gcatcgactg
    19501 ggatgacgac gatcatgacc atcatcatca tgacgatgat gattatcatc accacgatgg
    19561 cggtcatcgt gacggtaatg gctggcaaca caacggcgac aacatcaata tcgacgtcaa
    19621 caatttcaac cgtatcaccg gtgagcatct tactgataag aatatggcat ggcggcacaa
    19681 tccaaactac cgtaatggta tgccctatca tgatcaggat atggcaaagc ggtttcatca
    19741 aaccgatgtc aacggcggaa tgagtgccac gcagctacct gctccaacac gcgacagcca
    19801 gcgtcagacg gcagcaagtc agtttcagca acgaacacac gccgcccccg tcattacacg
    19861 agatacccaa cgtcaggcag cggcacagcg gtttaatgaa gcggagctat gacgacttcc
    19921 gcgacttcag ccgtcgccaa cccctgaccc agcaacaaaa ggacgccgct cgtcagcgtt
    19981 atcagtcagc ttctcctgag cagcgccagg tgtttaagga aaaagtacag cagcgcccac
    20041 tgaaccaaca gcaacgtgat aacgcccgcc agcgtgttca atcagcatca cctgaacaac
    20101 gtcaggtttt tcgggagaga gttcaggaga gccgcccaca acgtctaaac gacagtaacc
    20161 gtactgcaag attgaataac gatcaacggt cagcagtacg cgaacgtctc tctgagcgcg
    20221 gagcaaggcg actggaaagg taaattacag acgtaaaaaa agcggcgtgg ttagccgctt
    20281 ttttaattgc cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg
    20341 caggtcggta atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt
    20401 cgggtgcgca tggatggtca gcgcgatgtc ttcagcgtca caacccattt cgattgccag
    20461 gccgatttca cccagcagct cgccgccgtt ggtaccgaca atcgcaccac cgatcacacg
    20521 gtgagattct ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcgg aagcgatagc
    20581 acgaccagaa gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc
    20641 ttctttctca gtcagaccta cccatgcaac ttctggttcg gtataggcga tagacgggat
    20701 aactttcgga tcgaagtagt gttttttacc ggcgataact tcagcggcaa cgtgaccttc
    20761 gtgaacacct ttgtgtgcca gcatcggctg accaacgata tcgccgatag caaagatgtg
    20821 cggtacgttg gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt ccacttccac
    20881 gccagctttg cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac
    20941 ggcgtcgtaa cgctgcggtt cagcgggtgc ttttttgcct tccatcgtca cataaatacc
    21001 gtcttctttc gcttcaacag cggtaacttt ggtttccagc atcaggttga atttcttgct
    21061 gatacgcttg gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa
    21121 catttcaacc acgtcaatct gtgaacccag cgcgtggtat acggtgccca tttccagacc
    21181 gatgataccg ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc
    21241 agtggagtcc cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga
    21301 acccgctgca atgatcgcgt tgtcgaagtt gatcacggtt ttaccgttct caccttcaac
    21361 ttccagggtg ttagccccgg taaatttacc cagaccgttg accactttga ctttgcggcc
    21421 tttcgccata ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg
    21481 aatcttgtca atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt
    21541 ggcttcttcg ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt
    21601 caggcaaaca ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc
    21661 agcgcaacgg aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt
    21721 tttgatttca gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt
    21781 ctttcaggcc gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc
    21841 ccctggggat tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag
    21901 taatacccta accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa
    21961 tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg
    22021 agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg
    22081 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
    22141 cacgcggtgg tcgaaggaga gagaaagcgg cagcatcaga cgcggcacga actctttacc
    22201 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt
    22261 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc
    22321 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
    22381 ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag
    22441 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
    22501 accgtcttcc gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc
    22561 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc
    22621 gttctgctgt ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac
    22681 atgcgggatc atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc
    22741 cagttccact tcttcgattt caccaaactt gctgaagtcc accttcggcc acggcagcat
    22801 gcctgggata ccgccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt
    22861 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
    22921 cgccaggtta acaccaaact cgcgtgccag acggcggatc agcggagtcg cgtgaacata
    22981 agcgtcgttt tcagcaaatt cagatttgcc ttccgctttc gcagctggtg ctgctgccgg
    23041 ggcttcagct tttgctgccg gtgccggcgc tgccgcttcc tgtttcgcag gagctgccgc
    23101 aggcgctgcg ccttcaactt cgaagatcat aatcagcgag ccagttttca ctttatcgcc
    23161 aacgttgact ttcagttcct tcacgacgcc tgcaaacggc gccggaactt ccatagaagc
    23221 tttgtcgcct tctacggtga tcagtgactg ttcagcggca actttgtcgc ccactttcac
    23281 catcacttca gtcacttcaa cttcgtcacc gccgatatcc ggaacgttaa cttctttcac
    23341 gccagctgct ggtgcagggg ccgctgccgg agccgcttcc tgtttagccg ccggagctgc
    23401 cgcgcctgct tcacccgcga cttcgaagac cataatcagc gagccggtag acactttgtc
    23461 acccacgttc actttgatct ctttcacggt gccagcaaac ggagccggaa cttccataga
    23521 agctttatcg ccttctacgg tgatcagcga ctgttcagct tcaactttat cgccaacttt
    23581 caccaggatt tcggtcactt caacttcgtc gctgccgata tccggaacgt taacgtcttt
    23641 tgccgccgca gccgcgggtg ctgctgccgg agctgcttct ttcttctctt ctgcctgagc
    23701 aggtgcagcg tctgctgcac cgtcggcgga atcgaaaatc ataatcagtg cgccggtctg
    23761 ggttttatcg ccaacagaga ctttgatctc tttaacgata cccgcctgcg gagacggaac
    23821 ttccatagag gctttgtcgc cttctacggt gatcagcgac tgttcggctt caactttgtc
    23881 gcccactttg accaggatct cggtgatttc aacttcatca gccccgatgt ccggtacttt
    23941 gatttcgata gccattattc ttttacctct tacgccagac gcgggttaac tttatctgca
    24001 tcgatgttga atttggcgat tgcgtcagca accactttct tatcgatttc gccacgttta
    24061 gccagttcgc ccagcgccgc aaccacgaca taagaagcat caacttcgaa gtggtgacgc
    24121 aggttctcac ggctgtcgga acgaccgaag ccatcagtac ccagtacgcg gtagtcgtca
    24181 gccggtacgt aagtacggac ctgctcagcg aacagtttca tatagtcggt agatgccact
    24241 gccggagcgt cgttcatcac ctgagcgata tacggaacgc gcggagtttc cagcggatga
    24301 agcatgttcc agcgttcaca atcctgacca tcacgcgcca gctcggtgaa ggaggtcacg
    24361 ctataaacgt cagaacctac gccgtaatct ttcgccagga tctctgccgc ttcacggacg
    24421 tgacgcagga tagaaccgga gcccagcagc tgaactttac ctttgctacc ttcaatggtt
    24481 tcgagtttgt agataccttt acggatacct tcctcagcac cttccggcat tgccggcatg
    24541 tggtagtttt cgttcagcgt ggtgatgtag tagtaaacgt tctcttgttt ttcaccgtac
    24601 atacgctcca gaccgtcatg cataatgaca gcaacttcgt aagcgtaagc cgggtcgtaa
    24661 gagatacagt tcggaatagt cagcgactga atgtggctgt gaccatcttc atgctgcaga
    24721 ccttctccgt tcagggtggt acgaccggaa gtaccgccga tcaggaagcc acgcgcttgc
    24781 tggtcgccag ccgcccagca cagatcgcca atacgctgga agccgaacat cgagtaatag
    24841 atgtagaacg ggatcatcgg cagattgttg gtgctgtaag aggtcgccgc tgccagccag
    24901 gaacaacctg cgcccagctc gttgatccct tcctgcagaa tctgaccttt ctcgtcttct
    24961 ttatagtaag caacctgctc gcggtcctgc ggggtgtact gctgaccgtt cgggctgtaa
    25021 ataccaatct gacggaacag accttccata ccgaaagtac gcgcttcgtc ggcgatgatc
    25081 ggtaccagac gatctttgat cgacttgttc ttcagcatta cgttcagggc acgaacgaaa
    25141 gcgatagtgg tagagatctc tttgctctgc tcttccagca gcgcgccgaa gtcttgcagg
    25201 ctcggcagct caagcttctc ggtgaagttc ggctgacggc ttggcagata accgtgcagt
    25261 ttctgacgct gtgcgtgcag ataggtatgc tcttcagaac cttccgggaa ggtgatgtac
    25321 ggcagttttt cgatatctgc atcagacacc ggcacattga aacggtcgcg gatgtgacgc
    25381 acgccgtcca tgttcatttt cttaacctgg tgcgcgatgt ttttaccttc agccgcgtcg
    25441 cccatgccgt aacctttaat ggaatgagca aggattactg tcgctttgcc tttggtttcc
    25501 tgcgctttct tgaatgcagc gtagattttc ttcggatcgt gaccaccacg gttcagtgcc
    25561 cagatctgct cgtcagtcca gtctgcaacc agtgctgcgg tttcaggata tttaccgaag
    25621 aagtgttcac gaacgtacgc accatctttc gatttgaagg tctggtagtc gccgtcaacg
    25681 gtttcgttca tcagctggat cagtttaccg ctggtatctt tacgcagcag atcatcccaa
    25741 cggctacccc acatcacttt gatcacgttc cagccagcac cttcgaagat gccttccagt
    25801 tcgttgatga tcttgccgtt accggtgacc gggccgtcaa gacgctgcag gttacagttg
    25861 ataacgaaga ccaggttatc cagtttttca cgggtagcga tggtgatcgc acctttagat
    25921 tccggttcgt ccatttcacc gtcgccgagg aacgcgtaaa cggtctgttt agaggtatct
    25981 ttcaggccac ggtgttccag atatttcagg aatttagcct ggtaaatagc accaatcggc
    26041 cccagcccca tagatacggt cgggaactgc cagaattccg gcatcagttt cgggtgcgga
    26101 taggaagaga ggccattgcc gtgaacttcc tgacggaagt tatccagctg ctcctgagtc
    26161 agacgacctt ccaggaaagc acgtgcgtac acgcccgggg agatgtggcc ctggaagtaa
    26221 accaggtcgc cgccatcctg ctcgttgcgt gcacggaaga agtggttaaa gcacacatca
    26281 taaatggttg cggaagactg gaaggacgcc atatggccgc ccagttcgag gtcttttttc
    26341 gacgcacgca gcaccgtcat gatggcgttc cagcggatag ctgaacgaat acggcgttcc
    26401 agttccagat tacccggata ctccggttgt tcttcaacgg ggatggtgtt gatgtagttg
    26461 ctgatacctg tgcctgcggc tacgtttaca ccgcctttgc gggcttcagc aagcagttgg
    26521 tcgatcagat actgagcacg ctcaacacct tcttcacgga tgaccgattc gatcgcctgg
    26581 agccagtcgc gagtttcgat cggatccacg tcatttggga aacgttctga catgggttat
    26641 tccttatcta tctaataacg ttgagttttc tggaacctgt cccattgaac tctcgccgga
    26701 aagctcaata agacaggttc tacgtttagt tgccgcgctt ttatatgcgc ttgatttaca
    26761 acatcttctg gataattttt accagaaaaa tcactaattc tttcgttgct ccagacgacg
    26821 cagagaacgc tcacggcggc tctcttcacg acttctgtcg agcaaaattt cttcgataaa
    26881 ggccagatgg cgatgcgatg cttcgcgcgc ttcttccggc ttaccggcca taatcgcttc
    26941 aaatatgcgg gtgcggtgac tactcaccag cggcagcatc tcgcgacgcg aatagagcaa
    27001 ttcgaagttc tggcggacat tctgggccaa catcggctcc atacacctta gcagatgaag
    27061 cagaaccaca ttgtgggccg cttcagtgac agcaatctga tactggagta cggcgtttga
    27121 ttccgcgtcc agatcgccag actgctgcgc cagttctatg gcgtgatgga gttcacagat
    27181 gcgttccttg tcttcatcgg tactacgcag cgcagcgtaa taagcggcga taccttccag
    27241 ggcgtgtcgt gtttcgagca agtcatactg tgactcagga tggtcggaga gcagctccac
    27301 cagcggatcg ctgaagcttt gccataggct gctctggaca aaagtgccgc caccctggcg
    27361 acgaagcaac aagcccttcg cttcgagacg ttgaatcgcc tcacgcaagg agggacggga
    27421 gacgtcaaac tgttttgcca gttcgcgttc cggtgggagt ttttcgcccg ggcggagagt
    27481 gccttcgagg atcaaaaact ccagttgctg ctcaatcaca tcggagagtt ttggttggcg
    27541 gattttgctg taggccatga gttcctgtct taagccactt gccgaagtca attggtctta
    27601 ccaatttcat gtctgtgacg ctaaagtaac aaagtattca ccttatgtcc atacaggttt
    27661 tgattgaaat catgaaactg tgcacatttt aacaacttga catatataac gtttcaaagt
    27721 tgtaactatg cacaaatgta gactttacgt aggaaaggga ttttttaacg attattaaac
    27781 tataaaaatc cgaattgaac cgattcactt accaattttg tgatttttaa ttcaattaaa
    27841 acgaatttaa attcattcta catattgaga ggggttgagg ctgagcttta caaacggttt
    27901 ctttttaagc aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca
    27961 ttattcttga tctgacggaa gtctttttgt aacaattcaa acttctttga tgtaaacaaa
    28021 ttaatacaac aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca
    28081 accaccgcac gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc
    28141 cttaaaaacc gccatattca gcttatcgcg ctgggtggcg cgatagggac cgggttattc
    28201 ctgggtagcg cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt
    28261 gctggtttta tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct
    28321 gtcgcaggct cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc
    28381 tctggctgga actactgggt actgtacgtt ttagttgcca tggctgagct gactgccgtg
    28441 ggtaaataca ttcagttctg gtatccggaa atccccacct gggtttctgc cgccgtattc
    28501 tttgtggtga ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc
    28561 tggtttgcca ttatcaaagt tatcgcggtg gtagcgatga tcatcttcgg cggctggctg
    28621 ctattcagtg gcaacagcgg cccgcaggcg accgttagca acctgtggga tcagggtggt
    28681 ttcctgccgc acggcttcac cgggctggtg atgatgatgg cgattatcat gttctcgttc
    28741 ggtggtctgg aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtata
    28801 ccgaaagcaa ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc
    28861 gttctgctct cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg
    28921 atcttccacg agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact
    28981 gcggcgctct ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg
    29041 gcacaacagg gtaatgcgcc aaaagcgctg gcgtctgtcg ataaacgtgg tgtaccagta
    29101 aataccattc tggtgtctgc actggtaacg gcgttgtgcg tactgattaa ctaccttgcc
    29161 ccagagtccg ctttcggact gttaatggcg ctggtggtat ctgcactggt aatcaactgg
    29221 gcgatgatta gcctggcgca tatgaaattc cgtcgcgcca agcaggaaca aggcgtggta
    29281 actcgcttcc ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg
    29341 gcggtactgg tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg
    29401 gtatggctga tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta
    29461 aaagcgcatt aatctctcta cgccctcacc cgtacagggt gagggcaata atctttacac
    29521 caacgccccg taaatcgtca gtagtgcaat caccaccacg accacgaacg aggttttctt
    29581 cgccattgaa accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag
    29641 agagaactgc gccagacgcg ttaacacctg atactgcgaa gtatggaagt cacccagcga
    29701 agcaaaccag gccggtaacg ctttctcccc atgaccgatc aaggcatata ccacacccgc
    29761 aagacgaacc ggcacccaat ccagtacatg aagcacggca tcaatgccgg actgtaaacg
    29821 atgatgcggc gtctggtatc gcgccagcca gtattgccat gcacgcaaaa acgcataccc
    29881 catcagcgta acgggtcccc agattccccc cacaatcaac cagaacagcg gagcaagata
    29941 aaaacgaaag ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcatc
    30001 gcagcctgcc gggacgccgt gaatcatggt gagttcgcca gccatcgtga cacgggcatg
    30061 gctatcatta cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc
    30121 gccaatacac agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac
    30181 tccctgcaat gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg tcatgcctaa
    30241 cgtgcgcccg agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag
    30301 ctgccagtgc tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa
    30361 tagcgtcatg ttgtctcctt gctgaccagc gcacgaaacc gcgcccaatc aaatgcaggg
    30421 ccgggatcgg ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata
    30481 tctggatagc gatcaatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg
    30541 gtatacgcca gcgtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt
    30601 tcgcgcccct gatactgaga gactcccgca tgccatgcac gtttatcgaa aggaacatac
    30661 tggactattt caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc
    30721 tcagcaaaga aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac
    30781 ggaccgccaa actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt
    30841 tcgtcatccg ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac
    30901 ccctgttcta acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta
    30961 gcatgtttct accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct
    31021 ataaccctga cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg
    31081 cggtggccca ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta
    31141 cggcaaaact tttaccggaa aactctcgct ctcatgccac ggtgatcacc cgcgagaatg
    31201 gcgtattttg cggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg
    31261 tcaccataat ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa cccttgttcg
    31321 aacttgaagg cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc
    31381 aaaccctttc aggagttgcc agtaaggtac gccactatgt cgaattactg gaaggcacca
    31441 acacgcagtt gttggatacg cgcaaaacct tacctggcct gcgttcagct ctgaaatacg
    31501 cggtactttg cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca
    31561 aagaaaatca tattattgcc tccggctcag tgcgccaggc cgtcgaaaaa gcgtcctggc
    31621 tgcacccgga tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaagccc
    31681 tgaaagcagg agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag
    31741 ccgtcaaacg caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa
    31801 cactgcgcga atttgccgaa acgggcgtgg actttatctc cgtcggtgcg ctaactaaac
    31861 acgtacaagc actcgacctt tcaatgcgtt ttcgctaatt gctgacagat ttatccgctc
    31921 catcgcgggc ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt
    31981 gtctttcgct gcaacgaaat aaaaaacctc ggaagctgct ttcccgtttc gttttttgcc
    32041 cctcgcttgt gctgatggcg tttgccaaag tagcgccaac caaatcaagg agcgaaacag
    32101 atggacaagc aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc
    32161 attttaagcg ccattggtat tcccgcttat caaaactacc tgcgcaaagc tgcactcacc
    32221 gacatgctac aaacctttgt gccttaccgt accgccgtag agttgtgcgc gctggaacat
    32281 ggtgg
//
LOCUS       DAJTMK010000027        31629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTMK010000027 DAJTMK010000000
VERSION     DAJTMK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31629
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.028"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..555
                     /locus_tag="OO135_001081"
     CDS             <1..555
                     /locus_tag="OO135_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCR5397012.1"
                     /translation="GRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWA
                     VTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEP
                     KNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALG
                     YRSPREYLRQRACNGLSDNRCLEI"
     gene            complement(676..1554)
                     /gene="hslO"
                     /locus_tag="OO135_001082"
     CDS             complement(676..1554)
                     /gene="hslO"
                     /locus_tag="OO135_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5397013.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(1579..1980)
                     /gene="hslR"
                     /locus_tag="OO135_001083"
     CDS             complement(1579..1980)
                     /gene="hslR"
                     /locus_tag="OO135_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5397014.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASETALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(1991..2659)
                     /gene="yrfG"
                     /locus_tag="OO135_001084"
     CDS             complement(1991..2659)
                     /gene="yrfG"
                     /locus_tag="OO135_001084"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5397015.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(2724..4859)
                     /gene="igaA"
                     /locus_tag="OO135_001085"
     CDS             complement(2724..4859)
                     /gene="igaA"
                     /locus_tag="OO135_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5397016.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTLVLQVPSPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            5179..5739
                     /gene="nudE"
                     /locus_tag="OO135_001086"
     CDS             5179..5739
                     /gene="nudE"
                     /locus_tag="OO135_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5397017.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(5904..8456)
                     /gene="mrcA"
                     /locus_tag="OO135_001087"
     CDS             complement(5904..8456)
                     /gene="mrcA"
                     /locus_tag="OO135_001087"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5397018.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            8576..9363
                     /gene="hofM"
                     /locus_tag="OO135_001088"
                     /pseudo
     CDS             8576..9363
                     /gene="hofM"
                     /locus_tag="OO135_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            9363..9860
                     /locus_tag="OO135_001089"
     CDS             9363..9860
                     /locus_tag="OO135_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HCR5397019.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQSQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDAQGRWQFEYQLTRKVS
                     DEHVL"
     gene            9844..10284
                     /gene="hofO"
                     /locus_tag="OO135_001090"
     CDS             9844..10284
                     /gene="hofO"
                     /locus_tag="OO135_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5397020.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            10274..10678
                     /gene="hofP"
                     /locus_tag="OO135_001091"
     CDS             10274..10678
                     /gene="hofP"
                     /locus_tag="OO135_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5397021.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            10590..11828
                     /gene="hofQ"
                     /locus_tag="OO135_001092"
     CDS             10590..11828
                     /gene="hofQ"
                     /locus_tag="OO135_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5397022.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            12229..12750
                     /gene="aroK"
                     /locus_tag="OO135_001093"
     CDS             12229..12750
                     /gene="aroK"
                     /locus_tag="OO135_001093"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5397023.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            12807..13895
                     /gene="aroB"
                     /locus_tag="OO135_001094"
     CDS             12807..13895
                     /gene="aroB"
                     /locus_tag="OO135_001094"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5397024.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            13987..15273
                     /gene="damX"
                     /locus_tag="OO135_001095"
     CDS             13987..15273
                     /gene="damX"
                     /locus_tag="OO135_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5397025.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAVTDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            15380..16216
                     /gene="dam"
                     /locus_tag="OO135_001096"
     CDS             15380..16216
                     /gene="dam"
                     /locus_tag="OO135_001096"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5397026.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLLGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            16234..16911
                     /gene="rpe"
                     /locus_tag="OO135_001097"
     CDS             16234..16911
                     /gene="rpe"
                     /locus_tag="OO135_001097"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5397027.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            16904..17662
                     /gene="gph"
                     /locus_tag="OO135_001098"
     CDS             16904..17662
                     /gene="gph"
                     /locus_tag="OO135_001098"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5397028.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            17655..18659
                     /gene="trpS"
                     /locus_tag="OO135_001099"
     CDS             17655..18659
                     /gene="trpS"
                     /locus_tag="OO135_001099"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5397029.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            18928..19833
                     /locus_tag="OO135_001100"
     CDS             18928..19833
                     /locus_tag="OO135_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397030.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            19850..20212
                     /locus_tag="OO135_001101"
     CDS             19850..20212
                     /locus_tag="OO135_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5397031.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            20296..21459
                     /locus_tag="OO135_001102"
     CDS             20296..21459
                     /locus_tag="OO135_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5397032.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            21459..22685
                     /locus_tag="OO135_001103"
     CDS             21459..22685
                     /locus_tag="OO135_001103"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5397033.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            22682..23560
                     /locus_tag="OO135_001104"
     CDS             22682..23560
                     /locus_tag="OO135_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5397034.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            23571..23924
                     /locus_tag="OO135_001105"
     CDS             23571..23924
                     /locus_tag="OO135_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5397035.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            23936..25240
                     /locus_tag="OO135_001106"
     CDS             23936..25240
                     /locus_tag="OO135_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5397036.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERMSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            25252..26337
                     /locus_tag="OO135_001107"
     CDS             25252..26337
                     /locus_tag="OO135_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5397037.1"
                     /translation="MKTFTLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(26489..27220)
                     /gene="phoB"
                     /locus_tag="OO135_001108"
     CDS             complement(26489..27220)
                     /gene="phoB"
                     /locus_tag="OO135_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5397038.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(27320..28105)
                     /gene="frlD"
                     /locus_tag="OO135_001109"
     CDS             complement(27320..28105)
                     /gene="frlD"
                     /locus_tag="OO135_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5397039.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLDENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(28102..28932)
                     /gene="frlC"
                     /locus_tag="OO135_001110"
     CDS             complement(28102..28932)
                     /gene="frlC"
                     /locus_tag="OO135_001110"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5397040.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(28982..29839)
                     /gene="frlB"
                     /locus_tag="OO135_001111"
                     /pseudo
     CDS             complement(28982..29839)
                     /gene="frlB"
                     /locus_tag="OO135_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
     gene            complement(29831..31159)
                     /gene="frlA"
                     /locus_tag="OO135_001112"
                     /pseudo
     CDS             complement(29831..31159)
                     /gene="frlA"
                     /locus_tag="OO135_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            complement(31455..31622)
                     /locus_tag="OO135_001113"
     CDS             complement(31455..31622)
                     /locus_tag="OO135_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5397041.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         7427 a         7987 c         8751 g         7464 t
ORIGIN      
        1 ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc
       61 tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca
      121 ctgcactggg cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg
      181 ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg
      241 gataatggtt catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt
      301 gaaccgaaga acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg
      361 aaaacgataa agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca
      421 aagaaccttg cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctgggt
      481 tatcgctcgc cacgggaata tctgcggcag cgggcttgta atgggttaag tgataacaga
      541 tgtctggaaa tataggggca aatccagtat ctgaccaaaa taatttgcga tttatttcat
      601 aggcgctggt aagcgcagca catcaggcca ttaagttgta aaatccggca gagccctctc
      661 tgccggacat actcattaat gaacttgcgg atctgccgga gacgcgttgt tgcggatttc
      721 agcaatatcc atcgcattga acagatagtg gttaccgcag taatcacaat gcatgtcaat
      781 ttcgccatct tccgccagga tgctatcaac ttcttcatca ggcagcgttt tcagcgcatc
      841 ggcgcaatgt tcacgcgagc aggtgcattt gaactccaca tcctgcggat cgtaaaccgt
      901 cacctcttct tcgtgataca aacgccacaa cacttcgttt gccggtaagg tcagcagttc
      961 ttcggttttg atggtttcgg ttagcgtcgc caggtggtca aagtcgtcct gctgggcatt
     1021 ttgcgcaggc attacctgca acaacatacc gcctgcagcc ggtttgccgt ctacgtcgcc
     1081 ggtgcgaata aacaggcgcg tcggcagctg ttcagaacgc ataaagtaat cttccaggca
     1141 ggccgccagg gtatcacctt ccagaccaac tacgccctga tagcgttcgc cttcgctcgg
     1201 ggtaatggtg atcaccacgt aaccattgcc gaccagcgtt ttcaggtcgg cattttctgg
     1261 aatttcgccc tgcacgcgcg ccacaccgcg catttgctgg ttattgttac cgttaataac
     1321 cgccagattc atcggaccgt cgccctgcag ctgtacggtg atatcaccat caaacttcag
     1381 cgtagcggtt aacaggctgg tcgcaaccag cagttctgcc agcacgtttt taacgggctg
     1441 cggataatcg tggttctcaa ggatctgttg cagggtttcc gaaacggtta ccagttcgcc
     1501 gcgaacggca aaattttcaa acagatagcg atgtaattgg tcatgttgcg gcataatcat
     1561 ctctcttgca ggtgacagtt attcactgtc gccgtgttta aatcgtaaca ggtcgcggcg
     1621 ctcttttttg tccgggcgtc ggtccgggtg cggcatggtt aaggcattaa gtttacgtgc
     1681 catcgccatt ttttcgcgtt tctctacact ttccgcagtc tcttcataca gcaaggctgt
     1741 ctcgctggcg gggcgacgct gttcagtaat cgcctttaca atcaccgtgc gttcgtcatt
     1801 tccctggcgc agagtgagcg tggcattcag ctcgacgatt ttgctcggct tgctgcgctg
     1861 cccgttgtaa tgcaccttac cgccttcaat catttcacgg gccagcgcgc gggttttata
     1921 aaaacgggca gcccatagcc atttatccag tcgaacctca acagcaggtt tctctttcat
     1981 ggcgtctcct tcacattagc gaggggatca ggcggcggta gtcattcagt gacggatggc
     2041 gttgatactg tttctcggca atcccggaat caggattagt cacgccgagg cagtaacgaa
     2101 taccaaattg cgcggcagca tcgagaatcg cttcgctgtc atcaataaac agcgttcttt
     2161 cagctttcag acccgtagct tcggccaccg catgccataa ccgctgatcc tctttcggat
     2221 aaccaaatgt gtgggtggaa agtaataaat caaggtgtgc gtccagaccc gtatgctcaa
     2281 gttttaccgc caggttgtgc ggatgagcat tggtgagcaa aattcgctgc ttaccgctgg
     2341 ctttcagtgc ctcaagaaac ggaatggtat cttcacgcag tacggcgcgc ggtcccatct
     2401 cggtggtcat tgcacagata tccagaccca gttgctcact ccagtaatca agacagtacc
     2461 agtttagcgt atgctgtacg tcgtgatatt gctggcgcat atattccatc gcttcctgtg
     2521 gcgtaacccc gtttttcgcg ccccatgttt caggcaccag cttttgccag aaatagttat
     2581 cgaaggcgag gtcgagcaac gtgccgtcca tatccagcag aacggtatct acgtcctgcc
     2641 aggcaatgtt gatatgcatg agggaaatct ccagagtgaa gcaatttgcg cgacagggta
     2701 gcataacctg ccgcgcaaac gtgttattcg ataaggcttt ctgaaggggt gatcagttgc
     2761 gggttcaggc agctttcata ataggcctga atctccatca tgcgagtgcg atgacgctgg
     2821 taacgacgcc aggcctgtac gccattgtaa atggcgctac ccagcatcgt cagtagcagc
     2881 aatgtggtgc tgagatagcg ccacaggccg gaacgatccg ggatcggatg aaggccaata
     2941 tgctgcgtac cattggcgtc agtgaagatt tttgtgacga taccttcggc gttaaacggc
     3001 gtatgcatca gcatttgtgc cagtttctgg aaagcgttcc actgttcttg cggcgggtag
     3061 tcgtaaagtg atgccgaagg ccagggctga tcaacaaaat cgctgccttc gtcgctgaca
     3121 atcaggaatc cgccgggggc cggactgttt agtgattgtg ccgctcgcgc cgtttcatgc
     3181 gtgataaacg gcgcggtgga ggttgccacc aggttatcca gcgattccgc actcaccggg
     3241 cgtaataaca cattcacgcc atcgagcttc ccggcgttgg cgcgttttac cagcgcgtcc
     3301 cagtctttac tgttgccgag attgaccagc gcatttttca gtcgcacaca gtcatctttg
     3361 gcagaacata aatccgctgt cttcagtaca atgtcgccaa aatcatcaag caataccatg
     3421 ccggattttt gaatcgctga acgtaatgag gcactgacgc gagattcatc ttccggttta
     3481 gggtgcagct ggcgattaac tgcttcagtc aatgccgtcg ctttgttgac cagttcagat
     3541 tctggtaatg gtaatgagcg ggcgtcattc cagatgatct gcgagcagtc aaacggtaaa
     3601 aaaggtgaat tggttttcgc gctccaggtt ccggaagtgc gaatattaca cattcccgta
     3661 ccgctaatac gcaatgtatc gcctacccgc acgccagcgt cagccagttg ttttacgctg
     3721 gtggcttcaa tggtctgcgc gcctttcatc catgagagag tgaatttcag cggcatatcc
     3781 agcgggatcc agaataacag cataaacagc accagcagcg agcccgcagc gataatcgta
     3841 ctgcgcagcc agtgctgtaa cggaaagttt tttacttcat catgcagcga aagatatcgc
     3901 ccctgacgca ctacgtggcg gtcgagatag atatcaatat cggtttgttg atcgagatcc
     3961 tgagcaatgt atggctgcca gtgtgcggga tagaccaggt cgataatacc gagtgaaata
     4021 ttgttgatct gttcctgatc gttttcgcca aacaatcccc aacgacgggg tgtaccgcgc
     4081 agacaatgaa tttcccgcag ggaggatttt gccgggggcg cgaatagccc ccacagacct
     4141 gcgcccagca gcagtaatgc gccgcctgcc agccacggaa caaatacatc cggggtaatc
     4201 aggcagaaaa agaacatcag gaaggaggcg acgatcagca acgcttcacg cagcccgcgc
     4261 ggacgactca gcgcatattc ttcatgcgtt tctttgcgaa tattgagtag ctcgatttgc
     4321 tcactctctt ccccgcgaat cgacgcctgc gtggacggaa caggttgcaa ggcatagccg
     4381 cgcgtttcct gcatgtactc ctgcagcgta tgaccgttga gtgaaataac caacggcagc
     4441 gaatcggtat gaatcagttc aacggtattc tcatcgttga tgtactgttc ccagaagggg
     4501 ggcaggtgga cttctaccga atcgaggtag taacgccatt tattcggatc gtcggtagag
     4561 atgccgtaac gtgtgatagc gtgtgtcagc atcatgacgt tgttgctttc ggcattcagc
     4621 gccagagaga tcggtgccgc gctagctccc gttgggctag gcacctgtaa taccagcgtc
     4681 aggctctcaa gataattttc aacggcgcta cgttcttcag gtgtgagttt acgcgtttgc
     4741 gcatccgcga aggcgttggt ccagggcagc tgacgccgtc tggatcgcac ttttatcagc
     4801 catcccgcaa gtagtgagca ggccagcaaa gcagctaaaa aaatcacaat ggtgctcatg
     4861 ctttccccat cttacttagt ctgtcaggcg tggtcagtcg caccagacac aatacatgat
     4921 tctgcggttg gctatcggca agcgtggagt ttaacttgag catcattaag cacatcacag
     4981 taaccgctga tcatagcaaa ccctctgaag gcggaatatc agcaaattcc tggagttatt
     5041 tcaacctatt gacttttaat ttgatttgag gacgattatt tattaagtta cataaatgta
     5101 aataacatgc aattcacatt gccgaaagcg tgtccgatat cgcacaataa ccgctgattt
     5161 cacagcatag atcccacgat gagcaaatca ttacaaaaac ccaccattct gaatgttgaa
     5221 actgtagccc gttcccgact gtttaccgtc gagagcgtgg atctggagtt cagcaatggc
     5281 gtgcggcgtg tttatgaacg aatgcgtcca accaaccggg aagcagtgat gattgtgccg
     5341 attgtggacg atcacctgat cctgatccgc gaatacgcag tgggaactga atcctacgaa
     5401 ttaggttttt cgaaaggatt aattgatccg ggtgaaagcg tctacgaagc cgctaaccgc
     5461 gagctaaaag aagaggttgg atttggtgcg aacgatctga cttttttgaa gaagctcagc
     5521 atggcaccgt cttacttttc cagcaaaatg aatatcgtgg tcgcgcaaga tctctacccg
     5581 gaatcgctgg aaggcgatga gccagagccg ctaccacagg tgcgctggcc gctggcgcat
     5641 atgatggatt tgctggaaga ccctgacttc aacgaagcgc gtaatgtcag cgcgctgttc
     5701 ctcgtgcgcg aatggttgaa agggcagggg cgagtgtaaa tgcgtgaagt ttgcctgatg
     5761 cacaacgctt atcaggctta tggggttcct gcaatatatt gatttttcac gattttgtta
     5821 gccggataac gcgttcaccc gcatccggca taaacaaatg cactttgtca gccaactgaa
     5881 aaggcgccga agcgcctttt taatcagaac aattcctgtg cctcaccatt atcgataatg
     5941 gtcgttccca cctcgtgcac cgcctgttgt gtcggctgcg taccttcgat gaaatactct
     6001 tcgcggctgt tgccaccatt agctaactgc ccggtgctgc gatcgatatt caccgtcaca
     6061 atacccggtg gcggcgtcag cggctgctcc ggcacacctt caagaacggc tttcatataa
     6121 gcgtcccatg caggctgggc actcttggct ccgccttcgt aacctgagat ctgatcttta
     6181 atcgctccgg aagccgttgt atgaccgaga ttacgacggt gatcatcaaa gccaatccag
     6241 accgaggtca caacgcccgg accgtaaccc gagaaccacg catctttcga actgttagtg
     6301 gtaccggttt tcccgccgat atcgcgacgc tgcaaatcac gacctgcacg ccagccagta
     6361 ccctgccagc ctggctcacc aaagatattg gtgttcaaag cactcttaat caggaatgcc
     6421 agcggagtgt tgatgacgtg cggtgcgtac tcctgcgcgc cagtcttcgc cactaacgcc
     6481 tgatttgcct gctccagctg cggcattggt acagaaacat tctgctgctc gcgggagata
     6541 gcgacatctt caacatcgtt attttccagc acgttcgatt tctgcgtatc accgtaaatc
     6601 accggaatat cgcattccgg gcaggctact ttcggtttcg cttcgaaaat cacgccgccc
     6661 tgatcgtttt caattttgct gataaaccac gggtccacca ggaagccgcc gttcgccatg
     6721 accgcgtagc cgcgcgccac ctgcattggg gtgaaggacg ctgaacccag cgccagcgat
     6781 tcggtgtgga caatgttttg cgccgggaag ccgaagcgtt gcagatattc tgcagcgtag
     6841 tcgacgccca tagcccgcat tgcacgtacc atcaccacgt ttttcgactg acccagcccc
     6901 tgacgtaagc gaattggacc agcatattgt ggcggcgagt ttttcggctg ccagtcagaa
     6961 ccggcacctg cgtcccagcg agaaattggc acatcgttca acatacttgc cagcgtcaga
     7021 cctttatcca tcgccgcggt gtagaggaac ggtttgatgt tggaacccac ctgacgcagt
     7081 gcctgggtgg cgcggttaaa cttgctctga ttgaaatcaa agccaccgac cagcgccata
     7141 acggcaccgt tttgcggatt gatcgacacc agcgccgagt tcacttccgg cacttgtgcc
     7201 agccaccatg catcgccaac ctgacgaacc cagatttgct gacccgtttg cagaacatcg
     7261 gtcactttac gcggcgtcgg tccctgctga gtatccgaac ggtaaggacg cgcccagcga
     7321 acgccttcca tactcaatgc gacggtcgac ccgtccgcca gcatcgccgt cgctccctga
     7381 ggattggcgc tggtgactgc ggcaggcagc agcggaccat aggtcggcag cgccttcagc
     7441 gtatcggtaa tcttgttgtt atcccacgcc gactcgccca ctttccacag cacatttgcc
     7501 gggccgcgat agccgtggcg catgtcgtag tccagcacgt tattacgtac cgcctgctgt
     7561 gcggcctgct gcactttgcg ggtgatggtg gtgtaaatgc gataaccgtc ttcataggca
     7621 ctttcgccat aacggttata catctcctgg cgcaccattt cgctcaggta cggcgcagag
     7681 aaagcaatct ccggcgcgtg atagttagcg ttaatcgcct cagtgcgtgt ctgatcgaac
     7741 tgttgttggg tgatataccc ttcatccagc atccgcgaca gtacgacgtt acgccgcgcg
     7801 acggcacgat ccattgagta gagcggatta aaggtggaag gcgctttcgg cagcccggct
     7861 atcacagcca tttcgttcag cgtcagttgg tcgaccgttt taccgaaata gacttgtgcc
     7921 gcagcaccga caccataggc gcggtaaccg aggtaaatct tgttcagata aagctcgagg
     7981 atctcgtctt tcgtcagcaa ctgttcaatg cgaatcgcga ggaagacttc cttaatctta
     8041 cgcatcagcg tgcgttctgg actgaggaag aagtttctcg ccagctgctg ggtaatggta
     8101 ctcgcccctt gtgacgcgtg gccggagaac agcgccacac ttgccgcacg gaagatcccc
     8161 accgggtcaa cgccgtgatg ctcgtagaag cggctgtctt ccgtcgcgat aaaggctttc
     8221 accatctccg gcgggatttg atccaacgta accggaatac gacgtttctc accgtattga
     8281 gcaatcagct cgccatcggc gctgtaaatc tgcatcggaa tttgcaggcg aacatctttt
     8341 aatgtcgcca catccggcag ttgtggctcg atgtagcggt ataggccata aatcgagcct
     8401 gctcccagca gaatgcaaca gactgcaagg atcaaaaaat actttacgaa cttcactgga
     8461 aatttctcat ttagtttcat ttgggcagtt tataaacaaa cgcgcggtag tataaaggca
     8521 agccagacgc attgatatac ccgtcagagt gacgggtgat aaggagatca tcacaatggc
     8581 atttaagatc tggcaaattg gtttgcattt acaacagcaa gaagcggtag cggttgcgat
     8641 cgtgcggggc gcaaaagaat gcttttcgca acgctggtgg cggttgccgc tggagaacga
     8701 cattatcaaa gatgggcgga ttgttgatgc gcagcagctg gctaaaacgt tgttaccttg
     8761 gagtcgcgaa ctgccgcagc gtcatcacat tatgttggcg tttcccgcca gtcgcacatt
     8821 acagcggtac agcggtcatt tccgcgcccg tcgatgtccc ttggtgagcg ggagcaaacg
     8881 gcctggctgt cagggacgat ggcccgcgag ctggatatgg atccggactc cctgcgcttc
     8941 gattacagcg aagactcact cagtcccgcc tataacgtga ctgccgcgca aagcaaagag
     9001 ctggcaacgc tgcttactct ggcagaaagg ttgcgtgttc atgttagtgc gatcaccccg
     9061 gatgccagtg cattacagcg attcctgcct tttttacctt ctcatcagca atgtctggcc
     9121 tggcgtgata acgaacagtg gctgtgggcg acgcgctata gctgggggcg caaactggcg
     9181 gtggggatga ctagcgcgaa ggagctggcg gcagcgttat tcgttgatcc cgacagcgtc
     9241 gcgatatgtg gcgaaggcgg attagacccc tgggaggccg tttctgttcg tcagccgctg
     9301 ctaccgccgc ctggtggaga ctttgccatc gcgctggggc tggcgcttgg gaaggcgtac
     9361 tgatgaaccc gccaattaat tttttgccct ggcgacagca acgccggacc gcttttctgc
     9421 gtttctggtt gctgatgttc gttgcgcctc tgctgctggc cgtcgggata acgctaatac
     9481 tgcgtctgac aggcagcgcc gaagcgcgca tagacgcagt tttgcttcag gcggaacaac
     9541 aactcgcccg cagcttacag ataacgaagc cacgtttgct ggagcaacag cagttacgcg
     9601 aacagcgttc gcaacggcag agccagcgac aatttacccg cgactggcaa tctgcgctgg
     9661 aagcactggc ggcgctttta cccgagcacg cctggctgac aacgataagc tggcagcagg
     9721 gaacgctgga gatcaagggg ctgacaacaa gcattaccgc gttaaacgca ctagaaacgt
     9781 cactccgcca ggatgcgcag ggacgctggc aatttgagta tcagttaaca aggaaggtta
     9841 gcgatgaaca tgttctttga ctggtggttc gccacatcac cccgcctccg ccagttttgc
     9901 tgggcattct ggttgctgat gttagttacg ctcatttttc tgtcatcgac acaccatgaa
     9961 gagcgcgacg cattaattcg attacgggca agtcatcacc agcagtgggc cacactgtat
    10021 cgcctggttg acaccactcc cttcagcgaa gaaaaaacgc tgcccttttc gccactggat
    10081 tttcagttgc ccggcgcgca actggtttcc tggcatccat ccgcgcaggg aggtgagttg
    10141 gcgttgaaaa cgctgtggga agcagtgccg tcggcattca cacggctggc agagcgcaac
    10201 gtcagcgtga gccgtttttc gttaagcgtg gaaggtgatg atcttttgtt cacgctacaa
    10261 ctggagacgc cgcatgaggg ttaaacgctg gttgttggca ggtattgcat tgtgcctttt
    10321 aaccggtatg cgtgaccctt ttaaaccgcc ggaagatcta tgccggatta gcgaacttag
    10381 ccagtggcgc tatcagggga tggtagggcg aggcgagcgc atcatcggtg taataaaaga
    10441 cgggcaaaag aaatggagac gggtgcagca aaacgatgtg ctggaaaacg gctggacaat
    10501 tttacagctg acgccagacg tactaacgct gggtaccggg acaaactgcg aaccgccaca
    10561 atggttgtgg caacggcaag gagatacaaa tgaagcaatg gatagccgca ctactgttga
    10621 tgctgatacc cggcgtacag gcggcaaagc cgcaaaaagt gacgctgatg gtggatgacg
    10681 ttccggtagc tcaggtgttg caggcgctgg ctgaacagga gaagttgaac ctggtcgtgt
    10741 cgccagacgt cagcggtacg gtgtcgttac atctaacaga tgttccctgg aagcaggcac
    10801 tacaaactgt agtgaaaagc gccggactga taacgcggca ggaaggcaac attctctcag
    10861 tgcattccat tgcctggcag aataacaata tcgcccgcca ggaggcggag caggcgcggg
    10921 cgcaggcaaa tctgccgctg gaaaatcgca gtataaccct gcaatacgcc gacgcgggag
    10981 aactggcgaa agcgggggag aagctactga gtgccaaagg gagtatgacc gtcgataaac
    11041 gcaccaatcg ccttttgcta cgagataaca aaacggcgtt aagcgcgctt gaacagtggg
    11101 tagcgcaaat ggatctgccg gtcgggcagg ttgagctgtc ggcgcatatt gtcaccatta
    11161 atgaaaaaag tttgcgtgag ttaggcgtga aatggacgct ggccgatgcg caacacgctg
    11221 gtggcgttgg gcaagtcacc acgcttggta gcgacctctc cgtagcgacg gcgacaacgc
    11281 atgtcggttt taacattggg cgcatcaacg gacgcttgct ggatcttgag ctttccgcgc
    11341 tcgaacaaaa acagcagctg gatattatcg ccagtccgcg tctgctggcc tcacatcttc
    11401 agcctgccag cattaaacag gggagcgaaa ttccatatca ggtttccagc ggggaaagtg
    11461 gcgcgacgtc ggtggaattt aaagaggccg tcctggggat ggaggtcacg cccacggtgt
    11521 tacaaaaagg tcgcatccgg ctgaaattac acatcagcca gaacgttccg gggcaggtgc
    11581 tacagcaggc cgatggcgaa gtgctggcga ttgataagca ggagatcgaa acgcaggtcg
    11641 aggtcaaaag cggagaaacg ttggcgctgg gcggcatttt tacccgtaaa aataaatcgg
    11701 gtcaggatag cgtaccgttg cttggcgaca ttccctggtt cgggcaatta tttcgtcatg
    11761 acggaaaaga atatgaacga cgcgagttag tggtgtttat cacgccacga ctggtttcca
    11821 gtgagtaaac agccgtaaaa gcggtaatgt ttttatgctg aacgtgtttc atctatttga
    11881 cgcgcgcagg tatttagcat acaaggagta ccgatttgag agttggtgct cttcgctgcc
    11941 tgcgttccat gatgatgatt tatcattcag gcggcatttt gctgtctttt ttacgctaat
    12001 cttacccggt gatttatcgc cagagcggtg gtagcaaggc agcgcgcttg cagcgaccag
    12061 atatgcagag ggatgggtga tttattcagt tgccaaaccc gctggagtat tgagataatt
    12121 ttcagtctga ctctcgcaat atcttatgag gtttcagttc atgtcctgcg gcgctctctg
    12181 agcgaagcgg gtttatcatt aacgaatagt cttagtagta ccgaaaaaat ggcagagaaa
    12241 cgcaatatct ttctggttgg gcctatgggt gccggaaaaa gcactattgg gcgccagtta
    12301 gctcaacaac tcaatatgga attttacgat tccgatcaag agattgagaa acgaaccgga
    12361 gctgatgtgg gctgggtttt cgatttagaa ggcgaagaag gcttccgcga tcgcgaagaa
    12421 aaggtcatca atgagttgac cgagaaacag ggtattgtgc tggctactgg cggcggctct
    12481 gtgaaatccc gtgaaacgcg taaccgtctt tccgctcgtg gcgttgtcgt ttatcttgaa
    12541 acgaccatcg aaaagcaact tgcacgcacg cagcgtgata aaaaacgccc gttgctgcac
    12601 gttgaaacac cgccgcgtga agttctggaa gcgttggcca atgaacgcaa tccgctgtat
    12661 gaagagattg ccgacgtgac cattcgtact gatgatcaaa gcgctaaagt ggttgcaaac
    12721 cagattattc acatgctgga aagcaactaa ttctggcttt atatacactc gtctgcgggt
    12781 acagtaatta aggtggatgt cgcgttatgg agaggattgt cgttactctc ggggaacgta
    12841 gttacccaat taccatcgca tctggtttgt ttaatgaacc agcttcattc ttaccgctga
    12901 aatcgggcga gcaggtcatg ttggtcacca acgaaaccct ggctcctctg tatctcgata
    12961 aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga tagcgttatc ctccctgacg
    13021 gcgagcagta taaaagcctg gctgtactcg ataccgtctt tacggcgttg ttacaaaagc
    13081 cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg cgtagtgggc gatctgaccg
    13141 gcttcgcggc ggcgagttat cagcgcggtg ttcgtttcat tcaagtcccg acgacgttac
    13201 tgtcgcaggt cgattcttcc gttggcggca aaactgcggt caaccatccc ctcggtaaaa
    13261 acatgattgg cgcgttctac cagcctgctt cagtggtggt ggatctcgac tgtctgaaaa
    13321 cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt catcaaatac ggcattattc
    13381 ttgacggtgc gttttttaac tggctggaag agaatctgga tgcgttgttg cgtctggacg
    13441 gtccggcaat ggcgtactgt attcgccgtt gttgtgaact gaaggcagaa gttgtcgccg
    13501 ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct gggacacacc tttggtcatg
    13561 ccattgaagc tgaaatgggg tatggcaatt ggttacatgg tgaagcggtc gctgcgggta
    13621 tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt tagttctgcc gaaacgcagc
    13681 gtattataac cctgctcaag cgggctgggt taccggtcaa tgggccgcgc gaaatgtccg
    13741 cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt ccttgcggga gagatacgct
    13801 taattcttcc gttggcaatt ggtaagagtg aagttcgcag cggcgtttcg cacgagcttg
    13861 ttcttaacgc cattgccgat tgtcaatcag cgtaacaaca agaaaggtca ggccgcttat
    13921 caagcggtct attagcttca ggttaattgc aacgtggtaa gcattaacct tttagtgggg
    13981 tgttaaatgg atgaattcaa accagaagac gagctgaaac ccgatcccag cgatcgtcgt
    14041 actggtcgtt ctcgtcaatc ttctgaacgt tctgagcgta ctgaacgtgg cgaaccgcag
    14101 atcaattttg atgatattga acttgatgac actgacgatc gccgtccgac tcgtgcgcaa
    14161 aaagagcgca atgaggaacc ggaaatcgaa gaagaaattg acgaatccga agatgaaacc
    14221 gtggatgaag agcgcgtaga gcgtcgtccg cgtaagcgca aaaaagcagc cagtaaaccc
    14281 gcttctcgtc agtatatgat gatgggcgtc ggcattctgg ttctactgct gttgatcatc
    14341 ggtatcggtt ctgcgctaaa agccccctcg acctcttcca gcgatcaaac cgcgtctggc
    14401 gagaagagta ttgatcttgc aggcaatgcg accgatcagg cgaatggggt gcagccagcg
    14461 ccgggaacca cgtctgcgga aaatactcag caggatgttt ctctgccacc gatctcttct
    14521 acgccgactc aagggcaaac cccggcggta acggatggtc aacaacgtgt tgaagtgcag
    14581 ggtgacctga acaatgcgct gactcagcca caaaatcagc aacagttgaa caatgtggcg
    14641 gtcaattcca cattgccgac cgaacccgcg actgtcgcgc ctgttcgcaa tggcaatgca
    14701 tcgcgtgaca cggcgaaaac gcaaaccgct gaacgtccgt ccactacgcg cccagctcgt
    14761 cagcaggcgg tgattgaacc gaaaaaaccg caagcaaccg tgaaaacgga gccgaagccg
    14821 gtagcacaga cgccgaagcg tactgaacca gctgcccctg tggcgagcac gaaggcaccg
    14881 gctgcgactt ctacgccagc accaaaagag acggcgacta cggctccagt acagacggca
    14941 tccccggcgc aaaccacggc aacaccagcc gctggaggga agaccgcagg taatgttggt
    15001 tcgttgaaat cggcaccgtc cagccattac actctgcagc tgagcagttc ctctaactac
    15061 gacaacctga acggttgggc gaagaaagag aatctgaaaa actacgttgt ctatgaaacg
    15121 acgcgtaatg gtcagccgtg gtatgtcctg gtttctggcg tgtacgcttc gaaagaagag
    15181 gcgaaaaaag cggtatctac attgccagca gatgttcagg ccaaaaaccc gtgggcgaaa
    15241 ccgctgcgtc aggtacaggc cgatctgaag taatcaagct tatctcccgc aatggtttat
    15301 cgttgcggga gctgcctgaa gcgctggatg ctgtcggagc tttctccaca gccggagaag
    15361 gtgtaattag ttagtcagca tgaagaaaaa tcgcgctttt ttgaagtggg cagggggcaa
    15421 gtatcccctg cttgatgata ttaaacggca tttgcccaag ggcgaatgtc tggttgagcc
    15481 ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt tctcgttata tccttgccga
    15541 tatcaatagc gacctgatca gtctctataa cattgtgaag atgcgtactg atgagtacgt
    15601 acaggccgca cgcgagctgt ttgttcccga aacaaattgc gccgaggttt actatcagtt
    15661 ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg gcggtactgt ttttatattt
    15721 gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg ctcggtgagt ttaacgtgcc
    15781 gtttggccgc tacaaaaaac cctatttccc ggaagcagag ttgtatcact tcgctgaaaa
    15841 agcgcagaat gcctttttct attgtgagtc ttacgccgat agcatggcgc gcgcagatga
    15901 tgcatccgtc gtctattgcg atccgcctta tgcaccgctg tctgcgaccg ccaactttac
    15961 ggcgtatcac acaaacagtt ttacgcttga acaacaagcg catctggcgg agatcgccga
    16021 aggtctggtt gagcgccata ttccagtgct gatctccaat cacgatacga tgttaacgcg
    16081 tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt cgacgcagta taagcagcaa
    16141 cggcggcaca cgtaaaaagg tggacgaact gctggctttg tacaaaccag gagtcgtttc
    16201 acccgcgaaa aaataattct caaggagaag cggatgaaac agtatttgat tgccccctca
    16261 attctgtcgg ctgattttgc ccgcctgggt gaagataccg caaaagccct ggcagctggc
    16321 gctgatgtcg tgcattttga cgtcatggat aaccactatg ttcccaatct gacgattggg
    16381 ccaatggtgc tgaaatcctt gcgtaactat ggcattaccg cccctatcga tgtacatctg
    16441 atggtgaaac ccgtcgatcg cattgtgcct gatttcgctg ccgctggtgc cagcatcatt
    16501 acctttcatc cagaagcctc cgagcatgtt gaccgcacgc tgcaactgat taaagaaaat
    16561 ggctgtaaag cgggtctggt atttaacccg gcgacacctc tgagctatct ggattacgtg
    16621 atggataagc tggatgtgat cctgctgatg tctgttaacc caggttttgg cggtcagtcg
    16681 ttcattccac aaacactgga taaactgcgc gaagtacgtc gtcgtatcga cgagtctggt
    16741 tttgacattc gactggaagt ggacggtggc gtgaaggtga acaacattgg cgaaatcgct
    16801 gcggcgggcg cggatatgtt cgtcgccggt tcggcaatct tcgaccagcc agactacaaa
    16861 aaagtcattg atgaaatgcg cagtgaactg gcaaaggtaa gtcatgaata agtttgaaga
    16921 tattcgcggc gtcgcttttg atctcgacgg tacgctggtt gacagtgctc ccggtcttgc
    16981 tgctgcggta gatatggcgc tgtatgcgct ggagttaccc gtcgcaggtg aagaacgcgt
    17041 tattacctgg attggtaacg gcgcagatgt tctgatggag cgtgcattgg cctgggcgcg
    17101 tcaggaacgt gcgactctgc gtaaaacaat gggtaaaccg cccgttgatg acgacattcc
    17161 ggcagaagaa caggtacgta ttctgcgtaa actgttcgat cgctactata gcgaggttgc
    17221 cgaagagggg acgtttttgt tcccgcacgt tgccgatacg ttgggcgcgt tgcaggctaa
    17281 aggcctgccg ctaggcctgg tcaccaacaa accgacgccg ttcgtcgcgc cactgctcga
    17341 agccttagat attgccaaat actttagcgt ggttatcggc ggcgatgatg tgcaaaacaa
    17401 aaaaccgcat ccggacccgc tgttactggt ggctgagcgg atgggaattg ccccacaaca
    17461 gatgctgttt gtcggcgact cacgcaatga tattcaggcg gcaaaagcgg caggttgccc
    17521 atcagttggc ttaacctacg gatataacta cggcgaggct atcgatctca gccagcctga
    17581 tgtaatttat cagtctataa atgaccttct gcccgcatta gggcttccgc atagcgaaaa
    17641 tcaggaatcg aaaaatgact aagcccatcg tttttagtgg cgcacagccc tcaggtgaat
    17701 tgaccattgg taactacatg ggtgcgctgc gtcagtgggt aaacatgcag gatgactacc
    17761 attgcattta ctgtatcgtt gaccaacacg cgatcaccgt gcgccaggat gcacagaagc
    17821 tgcgtaaagc gacgctggat acgctggcct tgtatctggc ttgtggtatc gatcctgaga
    17881 aaagcaccat ttttgttcag tcccacgtac cagaacatgc gcagttaggc tgggcactga
    17941 actgctatac ctacttcggc gaactgagcc gcatgaccca gtttaaagat aaatctgcgc
    18001 gttatgccga gaacatcaac gctggtctgt ttgactatcc ggtgctgatg gctgcggaca
    18061 tcctgctgta tcaaactaat ctggtaccgg tgggtgaaga ccagaaacag cacctggaac
    18121 tgagtcgcga tatcgcccag cgtttcaacg cgctgtatgg cgagatcttt aaggtgccgg
    18181 agccgtttat tccgaaatct ggcgcgcgcg taatgtcgct gctggagccg accaagaaga
    18241 tgtccaagtc tgacgataac cgcaataacg ttatcggcct gctggaagat ccgaaatcgg
    18301 tagtgaagaa aatcaaacgc gcggtcactg actccgacga gccgccggta gttcgctacg
    18361 atgtgcagaa caaagcgggc gtttccaacc tgctggatat cctttctgcg gtaacgggcc
    18421 agagcatccc agaactggaa aaacagttcg aaggcaagat gtatggtcat ctgaaaggtg
    18481 aagtggctga tgccgtttcc ggtatgctga ctgaattgca ggaacgctat caccgtttcc
    18541 gcaacgatga agccttcctg caacaggtga tgaaagacgg cgcagaaaaa gccagtgtac
    18601 acgcttcccg tacgctgaaa gcggtgtacg aagcgattgg ttttgtggcg aagccgtaat
    18661 atcaaaacgc tcggacggtc ccctcgcccc tttggggaga gggttagggt gaggggagca
    18721 ggcaagcgcc agtgggaatt accctttgct cgcattgtcg aataaatcac ttcgtcatca
    18781 ctctgaaacc ggggaaaccc ggttttttta tcctcatttg caataatccg aaaaaatgtg
    18841 aagcgcctcg ccgtttccac atctcacagt tgccacttat cttcatttta atgaagatat
    18901 aaacattcat tttattgaaa atatattatg cgtcgaacgt ttatcaaaaa agaaggcgtc
    18961 gtcatcacga cgctggcccg ttatttgttg ggtgaaaaat gcggtaatcg actgaaaacc
    19021 atagatgagc tggcaactga atgccgttca tcggttggcc tgacgcaggc cgcgttgaaa
    19081 acgttggaat caagtggagc gatacggatt gaacgccgtg ggcgcaatgg cagttatctg
    19141 gtcgaaatgg ataacaaagc gctgctgagt catgtggata tcaataacgt tgtgtgtgcc
    19201 atgcctttgc cctatacccg cttgtacgaa ggtttagcaa gcgggctgaa agcccagttt
    19261 gatggcattc ctttttatta tgcgcatatg cgtggcgcgg atattcgtgt ggagtgtttg
    19321 cttaacggtg tctatgacat ggcagtagtt tcccgcctgg cggcggaaag ttatctcagc
    19381 caaaacaatt tgtgcatcgc cctcgagctt ggcccgcata cgtatgttgg tgaacatcaa
    19441 ctgatatgcc gcaaaggtga gtctggaaat gttaagcgag taggtctcga tagtcgttcg
    19501 gcggatcaga aaatcatgac cgatgtcttt tttggcgata gtgatgtgga acgtgtcgat
    19561 ctctcttatc acgagagttt acaacgcatt gttaaaggcg atgtcgatgc ggttatctgg
    19621 aacgtggtgg cggaaaacga actgaccatg ctgggattag aggcgacgcc gctcacagac
    19681 gatccgcgat ttttacaggc caccgaagcc gtggtcctga cgcgagtcga tgattaccca
    19741 atgcaacaac tgctgcgtgc cgttgtagat aaacacgccc tgctcgccca tcaacaacgg
    19801 gtagtgagtg gggaacagga accgagttat taaacgaaag gcatctgata tggaaaccag
    19861 actcaacctg ctttgcgagg caggcgttat tgataaggac atctgcaaag gcatgatgca
    19921 ggtcgtcaac gtactggaaa cagagtgcca tctgccggtg cgcagtgagc aaggaacgat
    19981 ggcgatgaca catatggcga gtgcactgat gcgcagtcgc cgtggtgaag aaatagagcc
    20041 gctggataac gaattgctgg cagagctggc gcaatccagc cactggcaag ccgttgtgca
    20101 attgcatcag gtgttgttga aggaattcgc actggaagtt aatccgtgtg aagaaggcta
    20161 tttgctggcc aacctttatg gattatggat ggctgctaac gaagaggttt gaagctcccg
    20221 acaaatccac gtcgcacaac ttaaatattc aaaaaaatga atatttaatt caaggaacag
    20281 aaacgaggca acaagatgtt tgtagaagca ttgaaacgcc agaacccggc gctgatttcc
    20341 gccgcactaa gtttgtggca gcagggcaag atcgccccgg acagctgggt gatcgacgtg
    20401 gatcaggtac tggaaaacgg taagcgactg attgagacgg cgcggcttta cggcattgaa
    20461 ctgtatctga tgaccaagca gtttggccgt aacccatggc tggcggaaaa attgctggca
    20521 ttaggttaca gcggcattgt ggcggtggat tacaaagagg cgcgagtcat gcgccgcgct
    20581 ggtttgtcgg tggcgcatca ggggcatctg gtacaaatcc cttgtcatca ggttgctgac
    20641 gccgttgaac agggcaccga cgtcatcacc gtgtttactc tcgacaaagc gcgggaagtt
    20701 tctgcggcag cggtgaaggc cggacgtgtg cagtttgtgc tgctcaaagt ttatagcgac
    20761 gatgattttc tttatccggg gcaggagagc ggttttgtac agcattcgct gcacgaggtg
    20821 gttgctgaaa ttaagaaact gccgggtctg catttagccg gacttaccca tttcccttgc
    20881 ctgctttggg atgaggctgc cggaaaagtt ttgccgacac cgaatcttca cacgctgata
    20941 caggcacggg atcaactggc gaaatctggt attgcacttg agcaactgaa cgcgccttca
    21001 gcgaccagct gcacttcgct gccattactg gcgcaatacg gtgtgactca tgccgaaccc
    21061 ggtcatgcgc tgacgggcac tattccggca aaccagcagg gcgatcagtc tgaacgtatc
    21121 gcgatgctct ggttgagtga aatctcccat catttccgtg gcgacagcta ctgctacggc
    21181 ggcggttact accgtcgtgg tcatgcgcaa catgcgttgg tgtttacgcc agaaaatcaa
    21241 aagattactg aaaccaatct caaaactgtg gatgacagca gtatcgacta caccctgtcg
    21301 ctggcaggcg agtttccggt aagcagtgca gtggtgctct gtttccgcac gcagattttt
    21361 gtcacccgta gcgatgtggt gctggtgtcc ggtattcacc gtggcgaacc gaaaatcgtt
    21421 ggtcgttatg acagtcttgg aaactctctg ggggcgtaat ggcgcgattt gtggtgttag
    21481 tgattgatag ctttggcgta ggggcaatga aagatgtcac gctggtgcgt ccgcaagatg
    21541 cgggagcgaa tacatgtggt cacatcctga gccagttgcc gcatttgcag ctaccaacgc
    21601 tggagaagct ggggctaatc aacgcattgg gttatgcgcc aggcgatatg cagccgtcag
    21661 attccgcaac ctggggcgtg gcagagctgc aacatgaagg tggcgatacc tttatggggc
    21721 atcaggaaat tttaggcacg cgcccgttac cgccgctgcg gatgcctttt tgcgatgtga
    21781 ttgaccgtgt tgagcaggca ttagtatccg ccggttggca ggtggagcgc cgtggcgatg
    21841 aactgcaatt tctgtgggtc aatcaggcgg ttgcgattgg cgataatctc gaggcggatt
    21901 taggccaagt ctataacatt accgccaatc tctctgtgat ctcttttgac gacgcaatca
    21961 aaattggtcg tatcgtgcgt gagcaggtac aggtcggtcg ggtcattaca tttggtggcc
    22021 tgttaaccga cagtcaacgc attctcgatg ccgcagaaag caaagaaggg cgctttattg
    22081 gtatcaatgc gccgcgttct ggcgcttatg acaacggctt ccaggtcgtg catatgggct
    22141 acggtgtcga taaaaaagtg caggtgccgc aaaaactgta tgaggcgggc gtgccaaccg
    22201 tgctggtggg taaggtggca gatatcgtca gcaatcctta tggcgtgagc tggcaaaacc
    22261 tggtggatag ccagcggatt atggatatca ccctcgacga atttaacacc catccgacgg
    22321 cgtttatttg caccaatatc caggaaaccg acctcgctgg tcatgcagaa gacgtcgcac
    22381 gttatgccga acgtttgcag gtcgttgacc gtaaccttgc ccggcttgtt gatgcgatgc
    22441 agccagatga ttgcctggtc gtgatggcgg atcacggcaa cgatccgacc attggtcaca
    22501 gccaccatac ccgcgaagtg gtgccgctgc tggtttatca gcaaggcctg gctgctacgc
    22561 agctcggtgt gcgcaccacg ctttctgatg tgggagctac cgtgtgtgaa tttttccgtg
    22621 cgccaccgcc acaaaatggt cgctcttttc tttcctccct ccggtttgca ggagacaccc
    22681 tatgagtttt gatccgacgg gttacaccct ggcccatgag catctgcata ttgatctctc
    22741 cggctttaaa aacaacgtgg actgccgcct tgatcagtat gcgttcattt gccaggagat
    22801 gaacgacctg atgacccggg gtgtacgtaa tgtgattgag atgaccaacc gttacatggg
    22861 gcgcaatgcg caatttatgc tcggtgtaat gcgcgaaacc gggatcaacg tggtggcctg
    22921 tactggttat taccaggatg cgtttttccc ggaacatgtg gtgacccgca gcgtgcagga
    22981 actggcgcag gagatggtcg atgaaattga acagggtatc gatggcacgg agctgaaagc
    23041 cgggctcatc gcggagatcg gctccagcga aggaaagatt acgccgctgg aagagaaggt
    23101 atttattgcc gctgcgctgg cgcataacca gaccggacgc ccgatctcca cgcatacgtc
    23161 gttcagcacc atggggctgg aacaactggc tctgttgcaa gcccacggag ttgatctttc
    23221 gcgcgtcacc gttggtcact gcgatctgaa agacaacctc gacaacattt tgaagatgat
    23281 cgatctcggc gcgtacgtgc agttcgacac cattggtaag aacagttact acccggacga
    23341 aaagcgcatt gcgatgctcc acgcgctacg tgaccgtggg ttgctgaacc gcgtcatgct
    23401 gtcgatggat attacgcgcc gctcccattt aaaagccaat ggtggttatg gctatgacta
    23461 tttattgacc acctttattc cgcaattgcg ccagtcagga ttcagtcagg ccgatgtgga
    23521 tgtgatgtta cgtgaaactc cctctcaatt tttccaataa ggacagactc atgaaaaaga
    23581 ttggcgttgc aggcttacag cgtgagcaga ttaaaaaaac cattgaagcg acggctcctg
    23641 gcagttttga agttttcatt cacaacgaca tggaagcggc aatgaaagtg aaatccgggc
    23701 aactggatta ctacatcggt gcgtgtaata ccggtgcggg cgcggcattg tcgattgcca
    23761 tcgcggtgat tggctataac aaaagttgca ccattgccaa accaggcatt aaagcgaaag
    23821 acgagcatat cgccaaaatg atcgctgaag gaaaagtggc gtttggcctt tccgttgagc
    23881 acgtcgaaca cgcgattccg atgctgatta accatctgaa ataaaaggca cgactatgga
    23941 tctgtatatt cagattatcg tggtggcgtg cctgacgggt atgacatcgc ttctggcgca
    24001 tcgctcggcg gctgtttttc atgacggcat ccgcccgatc ctgccgcaac tgattgaagg
    24061 ctatatgaac cgtcgcgagg cggggagtat cgcttttggt ctgagcattg gttttgtggc
    24121 ctcggtgggg atctctttta ccctgaaaac cgggctgctc aacgcatggt tactctttct
    24181 tcctaccgat atcctcggcg tactggcgat aaacagcctg atggcgtttg gtcttggcgc
    24241 tatctggggc gtgttgatcc ttacttgcct gttgccagta aaccagctgc tgaccgcgct
    24301 gccggtggat gtattaggta gcctcgggga attaagctcg ccggtagttt ctgcttttgc
    24361 actgttcccg ctggtggcga ttttctacca gtttggctgg aagcaaagtc tggtcgccgc
    24421 cgtggttgtt ctgatgaccc gtgtggtagt cgtgcgctat ttcccacatc ttaaccctga
    24481 atccatcgaa atctttattg gcatggtgat gctgctgggg atcgcgataa ctcacgacct
    24541 gcgtcatcgt gatgaaaatg atattgatgc cagcgggctt tcggtgtttg aagaacgcat
    24601 gtcgcggatt atcaaaaact taccctatat cgccatcgtg ggagcattga ttgccgccgt
    24661 tgccagcatg aagattttcg ccggcagtga agtgtcgatc ttcactctgg agaaagcgta
    24721 ctccgcaggc gtaacgccgg aacaatcgca aacgctgatc aaccaggcgg cactggcaga
    24781 atttatgcgc gggctgggtt ttgtaccgtt gattgccacc accgccttag caacgggtgt
    24841 gtatgcagtt gcgggcttta cctttgttta tgcggtgggc tatctctcgc cgaatccgat
    24901 ggttgcagcg gtattaggcg cagtggttat ttcggcggaa gtcttgctgc ttcgttctat
    24961 cggcaaatgg ctgggacgct acccgtcggt gcgtaatgcg tcggataaca tccgtaacgc
    25021 catgaatatg ctgatggaag tggcgctgct ggtcggttcg attttcgcag caattaagat
    25081 ggcgggttat accggattct ctatcgcggt tgccatttac ttcctcaacg aatccctggg
    25141 ccgtccggta cagaaaatgg cggcaccggt cgtggcagta atgatcaccg gtatactgct
    25201 gaatgttctt tactggcttg gcctgttcgt tccggcttaa ggaaggcacc tatgaagacg
    25261 tttactctgc aaagcctgac gcttattgag gcgcagcaaa agcagtttgc gctggtggac
    25321 acgatttgcc gtcactttcc cggcgcggag tttctaacca gcggtgattt gggcttaacg
    25381 ccggggctga atcaaccgcg tattacccaa cgggtggagc aggtgctggc tgatgcattt
    25441 cacgcacagg ctgcggcgct ggtgcagggc gcggggactg gcgcgattcg cgccgggctg
    25501 gcagctttgc tcaaaccggg gcagcgtctt ctggtgcatg acgcgcctgt ttacccgacg
    25561 acacgggtta ttattgagca gatggggctg acgcttatta ctgttgattt caatgacctg
    25621 tcggcactga agcaggtcgt cgacgagcaa caaccggatg cggcgctggt gcagcatacg
    25681 cgccagcagc cgcaggacag ctacgtgctg gcagatgtgc tggcaacgtt gcgcgcggca
    25741 ggtgttccag cgttaaccga tgacaactat gcggtgatga aggtggcgcg aatcggctgt
    25801 gaatgcggcg cgaatgtctc gacattttcc tgcttcaagc tatttgggcc agagggtgtt
    25861 ggtgcagtgg tcggcgatgc tgatgttatc aaccgtattc gcgccacgct ttactccggc
    25921 ggtagccaga tccagggcgc acaggcgctg gaagtattgc gttgtctggt gtttgcgcca
    25981 gtgatgcacg cggtgcaggc aggggtatct gaacggttgc tggctttgct taacggtggt
    26041 gcggtgccgg aagtgaaaag cgcggtgatt gctaatgcgc agtcgaaggt gttgattgtc
    26101 gagtttcatc agccgattgc cgccagagtg ctggaagagg cgcaaaagcg cggtgccttg
    26161 ccttacccgg tgggtgcaga gtcgaaatat gaaatcccgc cgctctttta tcgcctttcc
    26221 ggaacgtttc gccaggcgaa tccacaatca gaacattgtg cgattcgcat taacccgaat
    26281 cgcagcggtg aagagacggt gctgcggatt ttgcgtgaga gtattgccag tatttaatgc
    26341 tggatagcac cgtcaagcta aattccgtac tgaacggtcc cctcgcccct ttggggagag
    26401 ggttagggtg aggggaatgt acgggatgta aaattcaacg cgccagcatc gccggatgcg
    26461 atgctggcgc atcttatccg gcctacggtt aatgtctttt attatcaata gttaaggtga
    26521 tgcgattggc gcggcagaag agttcggaga cgtgcaccgg tttgtcattc tgatcaaagg
    26581 cgattttgct gatgcgaaac aacggttcgc ccagttcgca tttcagccat tttgcctgcg
    26641 ggcgggtggc ggcaaagata tcgatggtct ttttgtcgct gaccactcgc gtgtcaaaac
    26701 gctcctgaaa taactgatag gtggagcttc cttcgacgta aatctcatca aagtcaggat
    26761 aacgcgacag cgggatccat gaactatcaa taaacagcgg ctctttatcg agatacatca
    26821 cccggcagag atggaacact tcgctgtttc cggggatgtt cagcttttcg caaaacggcg
    26881 cggcgctgac ccgttcctgt tcgatcactt tctctttcgt cgccttgcct tgtgagacgc
    26941 caaaatcggt aaaaccactg acagtaagca gggcgttttc aactttctgg ctttgtacaa
    27001 aggtgccttt tccttgccag cggatcagta cgccgtccgc cactaagtcg ctgatggctt
    27061 tgcgaatggt aatgcggctg acgttatatt gtgtacaaag ctcgttttcg gtagggatct
    27121 gttgcccggc ctggtaaacc ccctgcgcga tatcatccag cagtcgctgg cggacggtag
    27181 cgtagaggag ttgatgagag tagcggtccg tagctgacat aatcgaccct gaaataaaga
    27241 atgaattact cggattatag ggtcgttttc cggcaagtaa cagcacgttg gcgtgaagat
    27301 gctcacgcca acgttatacc taccaggcac cgtggtactg aatggttttc gccgcgcacg
    27361 ccgttccctg cgccatcgcc tgcggtaatg tcatccccgc agaccagccg caaaggaatc
    27421 cggcaatgaa cgaatctccg gcacccatgg tgtcgataac cgtcaccggt tcaggagcct
    27481 gacgccagaa ctgcgcgcca tcccaggcaa tgctgccgtt ttcatccagc gtgacaatca
    27541 ctgttcctgc gccacgggca acaatcgctt tcatcttcag acgcagcgtt tcgtcttctt
    27601 gcggtgcgga ggcaaaggca aaatcgagat gcggcaccag tgtctgccag agcgggctgt
    27661 cccacttgtc ggagaagtcg aaagcggtaa gtttgcccgc agcgtgcagc tgtgggaatg
    27721 cgtcttccgc atgtccccag attgccgcgt gcacaatgtc atactgcgcc agccaggcgt
    27781 aatcctcttc actcagggca aagtcggcca tcacgccttc ggtgtagtcg ccaaaaacgc
    27841 gatcattgtc gtgcagttcc acctgagttt gtgcggtaac gccgtgtttc gtatggacat
    27901 ggctgatatc gacgcccatg cgggcgagat cctgcttcag ctttgtgccg tagtcatcgt
    27961 cacccaccca ggtaatgcat cccggctgta tgccgtagcg agtgcagtac accgccacat
    28021 tgaccgcatt accgccagaa aacgctttat tcagttgcgg gtagatatcg acgcagttat
    28081 cgccgattgt cgccagggtt ttcatctctc atcctccggc agcagcgcgc gaaagcgttc
    28141 cagcgcctgg cgggcataga gtctgggctc gttcatatac atcgtcacca gctccaccgt
    28201 acagtaaccc tcatagcccc gctcaataat atcgcgcatc agttcccgca gcggcatttt
    28261 gccttctcca ggaatgtaat gcgtgtcgct ggccccgtcg ctgtcgacaa tatgcagatg
    28321 acgtaattta tcgcccagtt tgtcgaaata actcatcacc ggttccgcct ggacatacgg
    28381 cgcgcaaatg tcgaccatgc tgaacaggcg cggcgaaggc accagcgcca gcgcatgaag
    28441 cacatcatta gcattacaca cgacgttcga ttcatacggc gttaacggtt cgaggattag
    28501 atccatgccg atgttttccg cgtactcata cagttcgctt aggttctctg ccagccgtcc
    28561 ccagataaca ttaggtggcg tgagatagcc cgcgtgggcc gcggaaatca gcgtataacc
    28621 cgcgttcatc tcttttgcca tatccatcgc cagcttgatc atgtcgaggc tttcgcgacg
    28681 catatgttca tcgcccagca tcatgttata cggatagccg ttggtttctg gcgtatagcc
    28741 gataatcggc atctgatacg tctgcgccag cgccttgatt tgtttgatgc cgcccgcttt
    28801 taagtccggc gcgaacgcgt gcgggcgacc gccccaaatt tcgatgccgt cgtaacccag
    28861 ctcgcttgca tcacgaaatg cgtgttcaat aggcagccgc tggtggccgc aggtaaacat
    28921 acctgttttc atcgggtaac tccttgcgta aatccggccc gttgccgggc cgggcgaggg
    28981 attaatattc caccagacca ccgtaatagc ggcgttcatc cgggttgtga tctttgtaaa
    29041 tagacaggta gtagcagagc cactccattg gcacgaacat caggaacggt gccagccacg
    29101 ggtgcagccc ttgcgaaatt tcggcgtaat cgatgacgat cacgttgtca gtacgctgtt
    29161 taacaaagtt aatggcgcgt tcggtggtgt ggcgactttc atcattgccg agcaggaaca
    29221 ggaacggaac gcccggttcg acaatctcca gcgggccatg gcggaactct ccgctctcaa
    29281 tcacgcagcc gtgcgtccag gtaaattcca tcagcgttac aatgccttct ttgtaaccca
    29341 gcggacgcag cggacccgca gcaacggtat aaatcatcgg ccactggctg gccagttcac
    29401 caagctggcg gcctttttct tcccaggtgc gtaccagatg accgagcgca ttcggcaact
    29461 gcttgagatc gtttttgatc ttgccgattt ccgcgttcgg cgcgaggcgg gtgatcatct
    29521 ccagcaccac gctgtagcag agcagcaggt gaatttccca gatacagtcg gcctgataat
    29581 caatgctaaa ttccgccgcc gaggtaatcg ggctgtccgc gcgtttggtg aacgccgcag
    29641 tgagtgcgcc gcaggcccgg cccagctcca gcgctttgat tacctcttcc gttttaccgt
    29701 agtcagaaac gccaatcacc gcgcaacgat cgtcgaggcg atacggggtg ttatcgcaga
    29761 actcccagcc ggaaatggcg tagacctgaa gatcggaaaa gcgatccgcc aaatgtttcg
    29821 ccgcttgcgc cgcgttgagc tggcggctac gcttcgccca gaaggcgtaa gcaggcagac
    29881 cagtagcaat aacgatgacg gcgcagataa ggccgggaat cggtgcccag acaaaggttg
    29941 aggcgaccag aatgaggctt gacgcaatgg cgagggtggt catcagcccg aaagccggag
    30001 tacgccacag cggtttgtaa tcgtcgcgtt tacgacacca gatgatggag ccgaaggtga
    30061 gggtattttt gaaacacatc accagggtga aataacccag caggctggtg agatcggaaa
    30121 cgaagatgaa gaagagcccc agcgcccctt gcaggatgat ggagacatcc ggcgtgttgt
    30181 atttcggatg cacatggccg aagcatttaa agaacaggtt gtctttcgcc atcgcgtatt
    30241 ccagccgcgg ctggtacatc acgcagctgg aaagcgaacc aagaatgacg atcatcgccg
    30301 tgatggcaac aaagatccca gcggtgctgc cgagtgcggg gatccaggtc agggcgtcgg
    30361 aaatcggcgt ttcagaattg gcgagtttgt cgaagggcat caggtcggaa atcaccagcg
    30421 ccagcagggt gtagagcacc agaaccagca gacaggaacc aatcagcgct agtggcatgg
    30481 tttttccggg gtttttaatt tcgccggtca tataacagat agaggccatg ccggtatacg
    30541 accaactggt ggcagagatc cccgccagca gcgccataaa gctgcccgtt gcgccaatcg
    30601 cagtggtggt aggggcggca aaattctccg ctttaaacta gaagatccca aggccaatga
    30661 cgatagtgaa cgggataatt ttggcgatgg taattagcgt ctgaaacgct gcgccgcctt
    30721 caacggagcg caggtgtagc aacataaagg cgataattaa tccggcggcg ataaatttac
    30781 cgggcaacgg atcgataggc gttaaaaagc caagattgct gacaatcgcc agcgccataa
    30841 tcgacaatga cggcgcatcg ttggcccaga agctggccca gccggagagg aaagccagcg
    30901 gtcggcttcc ggcatttttc agataaacat aatctgcgcc attttccgga taagcggtgg
    30961 atagttccgc atagacgcac atttgcggga tcacaattaa cccgccaatg acaaacgtga
    31021 gcaccgtaag ccacggcgtg cccgctgctt ttgccacttc acccacagat acaaaaatac
    31081 cggagccgac ggttgtcccg acggcgattg caagaacggc ccaaaatccg agcttgcgtt
    31141 ggagttcctg gcttcccata gcgatacctt tttcttgtcc aacacgttgt aggggagagt
    31201 ttgcagtcat ttaaaaggta acatgacaat gcatgatgaa tataataaca tatgatgtta
    31261 tgtaatgcgg aggaagatca caaaatttcg cacaggatcg ctctgtggct aatgaatgta
    31321 gttatccaat tgaaattaaa gtgaaaatat ttttacgggt ggggagcaag aaggatatag
    31381 aaacaaatac gccctcggaa aatccagagg gcgtcgggca attaaaccgg tgttagccga
    31441 tttctgtcag agacttactg tgcagtagga ccgcaaccgc caatgatttt agaaatagaa
    31501 attgccgggt gcagcaggta gtcgtaagag cagttcttat cacggttttc aatatgacca
    31561 gtacatgcag ttaacgtagc cagtactgca cctaccagtg caactttaat aaatttgttc
    31621 atttacatt
//
LOCUS       DAJTMK010000028        31370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTMK010000028 DAJTMK010000000
VERSION     DAJTMK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31370
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.029"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(18..3299)
                     /locus_tag="OO135_001114"
     CDS             complement(18..3299)
                     /locus_tag="OO135_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5397042.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3417..4610
                     /locus_tag="OO135_001115"
     CDS             3417..4610
                     /locus_tag="OO135_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5397043.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCQETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4674..5927)
                     /gene="glyA"
                     /locus_tag="OO135_001116"
     CDS             complement(4674..5927)
                     /gene="glyA"
                     /locus_tag="OO135_001116"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5397044.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6255..7445
                     /gene="hmpA"
                     /locus_tag="OO135_001117"
     CDS             6255..7445
                     /gene="hmpA"
                     /locus_tag="OO135_001117"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5397045.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVERIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7490..7828)
                     /gene="glnB"
                     /locus_tag="OO135_001118"
     CDS             complement(7490..7828)
                     /gene="glnB"
                     /locus_tag="OO135_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5397046.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7889..9223)
                     /gene="glrR"
                     /locus_tag="OO135_001119"
     CDS             complement(7889..9223)
                     /gene="glrR"
                     /locus_tag="OO135_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5397047.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9213..9926)
                     /gene="qseG"
                     /locus_tag="OO135_001120"
     CDS             complement(9213..9926)
                     /gene="qseG"
                     /locus_tag="OO135_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5397048.1"
                     /translation="MRHIFQRLLLRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10090..11517)
                     /gene="qseE"
                     /locus_tag="OO135_001121"
     CDS             complement(10090..11517)
                     /gene="qseE"
                     /locus_tag="OO135_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5397049.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12075..15962)
                     /gene="purL"
                     /locus_tag="OO135_001122"
                     /gene_synonym="purI"
     CDS             complement(12075..15962)
                     /gene="purL"
                     /locus_tag="OO135_001122"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5397050.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16220..17776
                     /gene="mltF"
                     /locus_tag="OO135_001123"
     CDS             16220..17776
                     /gene="mltF"
                     /locus_tag="OO135_001123"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5397051.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGV
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEDIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="OO135_001124"
     CDS             complement(17773..18309)
                     /gene="tadA"
                     /locus_tag="OO135_001124"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5397052.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="OO135_001125"
     CDS             complement(18334..18969)
                     /gene="yfhb"
                     /locus_tag="OO135_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5397053.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19178..20026
                     /locus_tag="OO135_001126"
     CDS             19178..20026
                     /locus_tag="OO135_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5397054.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20082..20342
                     /gene="yfhL"
                     /locus_tag="OO135_001127"
     CDS             20082..20342
                     /gene="yfhL"
                     /locus_tag="OO135_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5397055.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="OO135_001128"
     CDS             complement(20536..20616)
                     /gene="shoB"
                     /locus_tag="OO135_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5397056.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21036..21416)
                     /gene="acpS"
                     /locus_tag="OO135_001129"
     CDS             complement(21036..21416)
                     /gene="acpS"
                     /locus_tag="OO135_001129"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5397057.1"
                     /translation="MAILGLGMDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="OO135_001130"
     CDS             complement(21416..22147)
                     /gene="pdxJ"
                     /locus_tag="OO135_001130"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5397058.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22159..22887)
                     /gene="recO"
                     /locus_tag="OO135_001131"
     CDS             complement(22159..22887)
                     /gene="recO"
                     /locus_tag="OO135_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5397059.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSSITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22899..23804)
                     /gene="era"
                     /locus_tag="OO135_001132"
     CDS             complement(22899..23804)
                     /gene="era"
                     /locus_tag="OO135_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5397060.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="OO135_001133"
     CDS             complement(23801..24481)
                     /gene="rnc"
                     /locus_tag="OO135_001133"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5397061.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPCVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="OO135_001134"
     CDS             complement(24754..25728)
                     /gene="lepB"
                     /locus_tag="OO135_001134"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5397062.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="OO135_001135"
     CDS             complement(25744..27543)
                     /gene="lepA"
                     /locus_tag="OO135_001135"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5397063.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="OO135_001136"
     CDS             complement(27741..28220)
                     /gene="rseC"
                     /locus_tag="OO135_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5397064.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="OO135_001137"
     CDS             complement(28217..29173)
                     /gene="rseB"
                     /locus_tag="OO135_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5397065.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="OO135_001138"
     CDS             complement(29173..29823)
                     /gene="rseA"
                     /locus_tag="OO135_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5397066.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="OO135_001139"
     CDS             complement(29856..30431)
                     /gene="rpoE"
                     /locus_tag="OO135_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5397067.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="OO135_001140"
     CDS             complement(30428..30583)
                     /gene="rseD"
                     /locus_tag="OO135_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5397068.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30839..>31370
                     /locus_tag="OO135_001141"
     CDS             30839..>31370
                     /locus_tag="OO135_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5397069.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTR"
BASE COUNT         7497 a         8398 c         7918 g         7557 t
ORIGIN      
        1 attacagtta atagggttta gtgaacgtag ttaaaaataa aaggcatcac agtggtgaat
       61 aatgccgctt tcggccaggc cggattaatc gccgtcaatt cagcgcgagc agactcatat
      121 tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca ccatcaggca tttttcttta
      181 tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag gttgtgaaac agcaaagaaa
      241 tccgcctcaa tactggtttt cgccatctct tgcgcccact gtttcatttc actaaacagt
      301 tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca ttccttgcca gaagagataa
      361 tcaaccggct gatcgttgta ataactgtgg atgttaatgg tgcgatcgcc ggtcgccgcc
      421 agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg cgttggcgca tatcgcctgc
      481 cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc cttcgcttaa attctccgga
      541 taatgcagcg cggcatgaag cagttcgctg gcctgctgcg gctctctggc atcaagatgc
      601 tgccaggcgc gtaataactg gttgaggata aactgactgg tgaccttccc ttccccgcct
      661 tcccacgggt ggaatttacg cgtggcgaga atgtccgccg ctttgtctgc ctgacccgtg
      721 agatgccaca aattgagcag ttctgcggtc atgtcgtcgc gtttcagcgc aatttccaga
      781 ttattttcca gacgcgccag tcgtttctcc ggtgtggctc cacttaactt atcaagcaaa
      841 tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct gataagcatt atcaagataa
      901 cgcgcggcca gctcataatc gtgttgctta ttccacgcat ggatcgctaa cccgcgccag
      961 ccgtcggcaa actccggcga catctctacg caacgttgcc aaaaggcaat ggctttgttg
     1021 tagctacgtt tgttgtagta gaagcaggcc agtaaatggc gagcaaacca gcactcttca
     1081 atactctcca gcgccgccac ttcttccagc gtattcggga aacggacaaa ctgcgggaag
     1141 acatcaatgg cttttgcgac cagttcgcca cgttcggctt tcggcagcaa gctggcttgc
     1201 aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca gcatctctgc cgccagggtg
     1261 ggcattcccc agttaatcag ttgcccggcg gtcatcaggg cgttaacgtc acgtccctga
     1321 cacagaccgc gccactgcgc gagagctgat tcgctacgac catcgaacca gttcagccac
     1381 cacagagtgg cgttcagcgg ataatcgcgc agcagtttct cgcgctgcac acgcgcgttg
     1441 tcctgacgac cacttaacac cagcagcaga ttatgcaggc aaagcacttc ctgattggtt
     1501 gggcaggcgc gaagactttg ttggcaaaaa tccagaccag cgtcgaagtt accattacgc
     1561 gccgccagtc gtgccaggcc ataatagcca ccggctttgc tgttgccgct ccagaccgca
     1621 cgccagaaat cctcttcggc ttgttgatat tgtccctgac gttcgtaagc actggcgcga
     1681 atcaaactcg cctgtccgca ctgcggattt ttgttcagcg catgtgcgcg tttcagagcc
     1741 tgagtggcat acgccaccgc ttgcgggaaa tcggcgcggt tatattccag catcgccagc
     1801 gccaggttac agcggtaatc cagcggatcc agcgccacgc cgcgcaggta gtaatcgaac
     1861 ggtgaacggc tggcgtgatg atattgctcc agatgctgac cgataaacca ggcttcatct
     1921 gtactggtaa tgtcttgtgc tgccagtggc gctttggcga cgtccagcaa cggcaactct
     1981 tgcggctgat gttcctgata actcagtaca atattgccgt cggcatcgga gagctcaatg
     2041 gtcagccttt caggattgat accgtgcaac acgccctgga tggcggtcgc aggcatcagt
     2101 gccacggcat catcaagtaa cgcgttgcat ttgccgattt cgcggatcgc caggcggtat
     2161 ccgttcaacg gagagatggc atacagcccc cactcaatcc cccgcttact acgctggagt
     2221 tttatcaccg catcgcggga ggcattttgc accatgccca aagaatgata aggcaggaaa
     2281 tactgctcaa aacgcttctc ttcgtaagca tcaagccagg taaaatcggg ctggttatcg
     2341 gcaaaaatac cggtcatcag ttcgatatac gggccgttat tgtcggtcag actcttatcc
     2401 cacgcctggc caaattcact gtgtccccaa ctccactgtt ttttacctgg cgcaatatgg
     2461 tggttggcaa cgtgcagcaa accgccatct tcatcgtgac accacgcgcc gacaaaatcg
     2521 tactgcgatt tttcggccat atatgaggtt ggaacaggca catttttata gcgagaaatg
     2581 tccactccgg cggagtagtc cactttgtag taagtgccgg tggcgatggg gaaagcggag
     2641 acggcccgtt tgccgtgatc aaacaccgca gtcacatccg gtgggaaaac gctctgatgc
     2701 ccttcccccc ctttcactgc cgggttggcc caccacaaga aatgacgcgg cgtggcgtta
     2761 ccgttataga cgcggctggc gatttccagc gccgcccggt cagggcgcag ggtgaaacct
     2821 gtcatcacct gtaaaccatg catcggctcc gtttcgccta cccacaccgt ctgtgcaccg
     2881 tcttcatggg cttcgagggt gaaatcaacg ggcataaagg tggtcgggcg atgatgttgc
     2941 ggccagttaa actcaatccc accggaaatc cacggcccca gcagccccac cagcgcaggt
     3001 ttaatgactt cattgtgata aacaaaatcg cgctgtttca ctttatccca cgcgcgatgc
     3061 acccgaccgc ccagttccgg caggatcatc actttgatgt agtcgttttc cagccacacc
     3121 gcctgccagg atttcagggt tttctgctcg ctcagcgtat cggtcacgcc gtagggataa
     3181 accgcgccgg acgatccctg ataaacgcga ttttccagga acatgggatg tatatcctgc
     3241 ggcccggttt cataggtcgg gatctcaacg cgctcttgcc acacttttac tggagtcatg
     3301 gtgccctcaa tattaacaag acatactgaa ttaaaagatt tgtggcagtg tattgaacaa
     3361 tctggcaatg ttttcgcgga ataatcacgc aattaactaa acaaggttta gtgaagatga
     3421 gagcctgcat taacaatcaa cagattcgcc accataacaa atgcgtgatt ctggaactgc
     3481 tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg gctggcgcaa atttcgattc
     3541 cggcagtcag taatattttg caggaactgg aaagcgaaaa acgggtggtg aatattgacg
     3601 atgaaagcca gacgcgcggg catagtagcg gtacatggct gattgcgccg gaaggtgact
     3661 ggacgctgtg cctgaacgtg acgcccacca gtattgagtg tcaggttgct aatgcttgtt
     3721 taagtccgaa aggtgaattt gagtatttac agattgatgc accgacgccg caggcgctgc
     3781 tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt gtggccggac cgcaccatca
     3841 atctggcgct ggcaatccac ggtcaggttg atccggtgac cggcgtgtcg caaaccatgc
     3901 cgcaagcgcc gtggacaacg ccggttgagg tgaagtatct gctggaagag aagctgggca
     3961 ttcgggtgat ggtcgataat gactgcgtga tgctggcgct ggcggagaaa tggcaaaata
     4021 attcgcagga acgggatttc tgcgtgatca acgttgatta cggcattggc tcgtcgttcg
     4081 tgattaacga gcaaatttat cgcggcagtt tgtatggtag cggacaaatt ggtcacacca
     4141 tcgttaatcc ggatggcgtc gtctgcgact gtggacgtta cggctgccag gaaaccgtcg
     4201 cctcgttaag tgcattaaaa aaacaggcgc gggtatggct aaaatcacaa ccggttaata
     4261 ctcagcttga tcctgaaaaa ctgacaaccg cgcagttaat cgctgcctgg cagagtggag
     4321 aaccgtggat caccagctgg gttgatcgct ctgccaatgc cattggtttg agtctgtata
     4381 acttcctcaa catcctcaat attaatcaga tttggttgta cggtcgcagt tgtgcctttg
     4441 gtgagaactg gcttaatact attattcgcc agacaggatt taacccgttc gaccgcgacg
     4501 aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca attaagccgc gcacaacagg
     4561 tgctgggaat tggctatttg tatgttgagg cgcagttacg acagatttga tggcgcgata
     4621 acgtagaaag gcttcccgaa ggaagccttt tttcaacaga ttaaccgttt cgcttatgcg
     4681 taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc gataacggct
     4741 tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt cgcttctgct
     4801 tctttgaagc cgcgacgcgt aattgccgga gtacccacgc gaataccgga ggtcacaaac
     4861 gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc acggcccaga
     4921 gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg
     4981 ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc taccatcgct
     5041 ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat cgcttctttc
     5101 agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc accagggaaa
     5161 acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccag gatcaggccg
     5221 ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc
     5281 gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc aacgaacagg
     5341 taagcaccga tgctgtcagc gatttcacgc attttcgccc agtccaccac gccggaatat
     5401 gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt ttccagatcg
     5461 gcgtagtcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta cagtttaccg
     5521 gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt catacccaga
     5581 acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg ggagccggag
     5641 tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc gatcgccagt
     5701 tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca
     5761 gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt
     5821 tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc catagcctgc
     5881 cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg catctcctga
     5941 ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa cggcgaacag
     6001 tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca aacgattacc
     6061 ttcaggctac gcaagacttt ggagaataaa gagcttgcaa ccggaaacgg atttcttttc
     6121 aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac caaagaacca
     6181 tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt aagatgcaaa
     6241 aaaaggaaga ccatatgctt gacgctcaaa ccatcgctac agtaaaagcc accatccctt
     6301 tactggtgga aacggggcca aagttaaccg cccatttcta cgaccgtatg tttactcata
     6361 acccagaact caaagaaatt tttaacatga gtaaccagcg taatggcgat caacgtgaag
     6421 ccctgtttaa cgctattgcc gcctacgcca gtaatattga aaacctgcct gcgctgctgc
     6481 cagcggtaga aagaatcgcg cagaagcaca ccagcttcca gatcaaaccg gaacagtaca
     6541 acatcgtcgg tgaacacctg ttggcaacgc tggacgaaat gttcagcccg gggcaggaag
     6601 tgctggacgc gtggggtaaa gcctatggtg tactggctaa tgtatttatc aatcgcgagg
     6661 cggaaatcta taacgaaaac gccagcaaag ccggtggttg ggaaggtact cgcgatttcc
     6721 gcattgtggc taaaacaccg cgcagcgcgc ttatcaccag cttcgaactg gagccggtcg
     6781 acggtggcgc agtggcagaa taccgtccgg ggcaatatct cggcgtctgg ctgaagccgg
     6841 aaggtttccc gcatcaggaa attcgtcagt actctttgac tcgcaaaccg gatggcaaag
     6901 gctatcgtat tgcggtgaaa cgcgaagagg gtgggcaggt atccaactgg ttgcacaatc
     6961 acgccaatgt tggcgatgtc gtgaaactgg tcgctccggc aggtgatttc tttatggctg
     7021 tcgcagatga cacaccagtg acgttaatct cttccggtgt tggtcaaacg ccaatgctgg
     7081 caatgctcga cacgctggca aaagcaggcc acacagcaca agtgaactgg ttccatgcgg
     7141 cagaaaatgg cgatgttcac gcctttgccg atgaagttaa ggaactgggg cagtcactgc
     7201 cgcgctttac cgcgcacacc tggtatcgtc agccgagcga agccgatcgc gctaaaggtc
     7261 agtttgatag cgaaggtctg atggatttga gcaaactgga aggtgcgttc agcgatccga
     7321 caatgcagtt ctatctctgc ggcccggttg gcttcatgca gtttaccgcg aaacagttag
     7381 tggatctggg cgtgaagcag gaaaacattc attacgaatg ctttggcccg cataaggtgc
     7441 tgtaatttga tgttgccgga tggaaacatc cggcaaccct tgacgcggtt taaattgccg
     7501 cgtcgtcctc ttcaccggta cggatgcgaa tgacccgtgc cacgtcaaag acgaagattt
     7561 taccgtcacc gattttgccg gtttgcgccg tgcgaataat ggtatcgaca caggtatcga
     7621 caatgtcgtc cggtacgaca atctcaattt tcactttcgg cagaaaatcc accatatact
     7681 ccgcgccgcg gtacagctcg gtatggcctt tctggcgacc aaagcctttc acttcggtca
     7741 ccgtcatgcc ggtaataccg acttcggcca gtgcttcgcg gacatcgtcc agcttgaagg
     7801 gttttataat cgcatcaatc tttttcatgc tattccttga aaaggtcgcc tgtcttttga
     7861 tctgctaaac gtaacacata acgccaattc attccttgaa atcgtttgca tccagctcgt
     7921 gtcgggaaag cagtttataa aattctgtcc ggttgcgccc cgccattctc gccgcgtggg
     7981 tgacgttgcc tttggtaatt tgcagcagct tacgcaaata gttgagttca aactgattac
     8041 gtgcctcaac aaaggttggc agcgccgtgt tttcaccctc cagcgcctgc tctactaatg
     8101 catcgctaat aaccggagat gaggtcagcg ccacgcactg ttcaatcacg ttgaccaact
     8161 ggcgcacatt acccggccag ctagcggtca tcaggcgttt catcgcatca gtggagaacg
     8221 cgcggacaaa cggtttatgt cgctctgccg cctggcgcaa caggtgattt gccagaagcg
     8281 gaatgtcttc tgtgcgctct gccagagccg gaattttcag gctgacaacg ttgaggcggt
     8341 aatagagatc ttcgcggaat tccccgcgcg ccatcgcttt tggcagatca cggtgagtgg
     8401 cagaaataat ccgcacattg atatcaatat cgcggttact gcccagcggg cgcactttac
     8461 gctcctgcaa cacgcgcagc agtttgacct gtaacggcgc aggcatatcg ccaatttcat
     8521 cgagaaatag cgttccgcct tccgccgcct ggaataaacc ttcgcgattg ctgacagcgc
     8581 cagtaaacgc gccacgcgca tgaccaaaca gctccgactc cagcaattgc tcgggcaatg
     8641 cgccacagtt aatagcaata aatggtttgc tgttgcgcgg gctggcgttg tggatagcct
     8701 gggcgaaaat ctctttcccg gtgccgctct gaccgttaat caaaacgctg acgtctgatt
     8761 gcgccaccag ccgcgcctgt tccagcaaac gcagcatcag cgggctgcgg gtgacaattg
     8821 cctcgcgcca gcgttcatcg gtggctggcg cggattgctc cagcgcatcg tcaattgcct
     8881 gatatagcgc gtctttgtcg acaggcttgg tgaggaaact aaaaacgccc tgctgtgttg
     8941 cagcaacggc atcggggata gaaccatgcg cagtaagaat aattactggc attcccggct
     9001 gcactttctg gatttcagca aatagctgca taccgtccat ttcatccatc cgcaggtcgc
     9061 tgataactaa atctactttt tcgcgattca gtacccgtaa tccttcagcg ccactttccg
     9121 ccgtgaccac actgtagcct tcgctggtca ggcgcaggcc aagcagtttc agcaatcccg
     9181 gatcgtcatc gaccaataat aaatgcgcag gtttatggct catcaggagt gacctcatgg
     9241 gtggatggcg cgggcttttc actctcgtgc ggcgtatccg gcgagaaatt tccagccggt
     9301 ttgcgggtcg agagctggcg ttcaatatcg gtcaggtttt ccagcttgcg ggtggtgagt
     9361 tccagttgct gctgtagaac gtgatgttgc tggcgcaatg tatccagctc gctgtcgctg
     9421 gactgctgga gtttactgta acgttggcgc tcttccgcca gttgcagttg cagcgcctga
     9481 ccgtcgcgcc agagttgata cagtgggcga acctgtgccg ggatctcggt acttaacgct
     9541 tcaatacgcg cgaccagctg gcggcgctca tacggcgtaa ttttggcgtc ggcgagcaaa
     9601 atcccttgtt taaaggtatt ttgccagctg ccgtcgtcat attggcgagc ttgctgacgc
     9661 gactgcgcag gcattaaacg atcagcacaa tccatcgccc gcagccagta aagcggattg
     9721 gtttcggttg atttaccttg cagcgcccag atgtcgctac attcagtaga aagatagtca
     9781 gccagttgat aaaccggaat tttttcttct gctggcgtat caatggctgg cttattgtga
     9841 ttctgcacgc aacccagcaa tgccagacat ggcagccctg ccagccacag ccgtcggagc
     9901 aataatcgtt gaaaaatgtg tcgcatattc accagactta aagcctatcc cagtgggcgt
     9961 aattgttgca gacagtctgg acatggcagc gcggagaaac cggagcgtac atatcgtacg
    10021 tgaagatttc gagcactgcc cggggccgaa ctgacaaata aaatagcctg ataggatagg
    10081 ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac aaacgtcttg
    10141 cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat ccctggcaat
    10201 gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa
    10261 gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat tgatgacatc
    10321 aatataaacc cgcgcaccat gtgaactgct gcgaaggcaa atgttaccgg attcagcccc
    10381 gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca ttggctccgc
    10441 caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc gtgcgggcag
    10501 gctatgagca gaaaccactg tctccaccag cggtgctaat tcaacattct ccagttccac
    10561 cgcactgtcc gcctgtttac ggttgtaatc aagtagttgt tcgatcagtt tttgcaaatt
    10621 gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa gcggcccgac
    10681 aacctggtca gccagtaatt cagtgccctc gcgcatactc gccagtggcg tttttaattc
    10741 atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca ggcgctcact
    10801 taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga acgaaacgct
    10861 attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat ttttcaccgg
    10921 cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa atagcaccag
    10981 cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt gctgcccacg
    11041 agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg caaaggcttc
    11101 aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt gggccagatt
    11161 gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc
    11221 atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa gcgtagggtc
    11281 gtccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc
    11341 ttcactacgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct gatcgctaag
    11401 cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggtagta gaattagcaa
    11461 aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt
    11521 gttactcttg tcagacgcga atagcgtgat gctaaccgag gggaagttca gatacaacaa
    11581 agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga cgtttcgccc
    11641 gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt gttgtgcgtc
    11701 attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa tgagccactg
    11761 cttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt actgattata
    11821 cgttgctttg agggatttat ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg
    11881 ctaaaaagag acaacttaag ataaacttat tagattgcaa taaaatcaat cacttaagtg
    11941 tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc
    12001 ccggggttgg gagcttatga tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc
    12061 aatgaactga tgaattaccc caactgctta cgcgcattgc ggaaaatgcg catccatggg
    12121 ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa aacacgttcc
    12181 gggtgcggca tcataatggt gactcgacca ctttcagtcg tgactgccgt aataccgttc
    12241 ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt atcgacatag
    12301 cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc acgcacttcc
    12361 acgcgccctt caccatgaga gacagcaatc ggcatttgcg agcccaccat cccctgcaac
    12421 agcagagacg ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag
    12481 gtattgcgca caaaacgtgg ccacaactca ctacctggga tcagttcgcg caggttagac
    12541 atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacggtg gaagaaggtt
    12601 gcaaactcat cgcgtacacg gtcattgaac aggattgact tcgcccaacc ttcaccggca
    12661 cccagcacat caccgtagga gaaaccaccg cacgcgacca gggcgtggaa atcttccagg
    12721 cccgtgcgtc cggtcagcag gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg
    12781 tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag tacggcaact
    12841 ttcggacgtg cgccagtggc aatatacggt gctgccacat cttcgttgat atcgaacgac
    12901 agttttacat tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc ctgatcggca
    12961 cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca ccagacacgc
    13021 aacgtggtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac aaaacggtca
    13081 ccggaaaccg cctgccctac ataatggaca caatcagcaa gcccatgctg tgccagtacg
    13141 gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag ttcttcgtta
    13201 aacaacgccg ccaggcgatc gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga
    13261 ccagcaaagg ccatttccgc cagcgttacc agcaggccgc catcagagcg gtcgtggtat
    13321 gcgagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc
    13381 gcgacgtcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg cgccagcgcc
    13441 gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcaatagtgc gttatcttcg
    13501 gtagaaagct gtggcgtgat ggtgtgacgc acatcttcca cgcgggcaaa tgcagaaatc
    13561 accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca gcgggttttc
    13621 atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca aagctcttcg
    13681 cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc tgccgccgcc
    13741 atccagttgg cggaaagttt aatgcgcttg atatcgccaa tctgtgttgc ggcaatattg
    13801 gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag cgcaaccggc
    13861 gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc
    13921 gcgcagttag cgaccggcac ctgccacgga cccaccatct gatcgcgcgc taccatgcca
    13981 gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt cggcaaatgc
    14041 agtatgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc atcgcccttc
    14101 gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag gacgtccagc
    14161 ggcagatcga tcggctgatt atcaaaatga cgatcgtgta gagaaagatg cagttcttcg
    14221 gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc gtcaaacagc
    14281 ggtaattgat cggcagcaac cgcgagcacg tagcgttcct gggattcgtt acaccagatt
    14341 tccagcgggc tcatgcctgg ctcgtcgctc agaatgtcgc gcagttcaaa tttaccgcca
    14401 cgcccgccgt cgctcaccag ttccggcata gcgttagaaa gaccgcctgc gccaacgtcg
    14461 tggataaaca gtattgggtt ggcatcacca agctgccagc aacggtcaat cacttcctgg
    14521 cagcgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca
    14581 gactgaccag acgccatgga agacgccgcg ccgccgccaa gaccgatgtt cattgccggg
    14641 ccgccgagaa cgaccagctt cgcgccaacg ttgatctcgc ctttttgtac gtgatcggcg
    14701 cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg cagctcttcg
    14761 ccgttgtggc tgttcacttt ttcttcataa gtacggaagt agccgttcag tgccggacga
    14821 ccaaattcgt tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg
    14881 gtgacaatgc gctcaggctt accgaaatct tcttcccatg gctgttcgaa gccaagaatt
    14941 cgcaggttgg aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc gcgcccggtg
    15001 gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca cggagaaatc
    15061 gccgtcgggt ggttgtgagt ttcgactttc atcagaatat gcgccggttc ctgatggaaa
    15121 tcatagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc ttccattacg
    15181 gcggcgttat ctttataagc agagagaacg tgatctggcg tggtttcgaa agtatttttg
    15241 atcatcctga acagcgattt cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa
    15301 attttgtggc ggcagtgctc agagttcgcc tgagcgaaca tatacagctc gatgtcgttc
    15361 gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc ttccgccaga
    15421 gccaggccaa gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc cagcaaatca
    15481 acgcttgtga ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc atctaaatca
    15541 aaaaagacag tttccatcat gcggtcgtgc agttcagcgg taacctgctg ccattgttca
    15601 ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt
    15661 tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga gatggtgcca
    15721 ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag tgccggacca
    15781 tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca
    15841 gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt
    15901 gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag aatttccatc
    15961 ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggaaacgg gcgtcattat
    16021 aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat aaaatcacta tcgtgaatta
    16081 gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg
    16141 ccgctcattt atgagtaaac ctttcactat tattacgttt tttcaagctg ggacgcgcac
    16201 gacacagaga attaactaat tgaaaaaatt aaagattaat tatctgttca tcggcattct
    16261 ggcactgctg ctcgcggtcg ctctctggcc atccattccc tggtttggta aagccgacaa
    16321 ccgtatcgcc gccattcaag cgcggggagt gttgcgtgtg agcaccattc atactcccct
    16381 gacttataac gaaatcaacg ggaaaccttt tggcctggat tacgaactgg cgaaacagtt
    16441 tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg cagaatatca gccagctgtt
    16501 tgacgacctt gataatggta acgccgacct gctggcggca ggactggtct ataacagtga
    16561 gcgggtaaaa aattatcagc ctggccctac ctattattcc gtgtcacaac aactggttta
    16621 taaagtgggt cagtatcgcc cacgtacgct gtgcaacctg acggcggagc aactcaccgt
    16681 tgcaccgggt catgtggtgg ttaacgatct ccagacccta aaagaaacaa aattcccgga
    16741 attaagctgg aaggtagacg acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga
    16801 aggaaaactc gattacacca ttgctgattc tgtcgccatc agcctgtttc agcgcgttca
    16861 cccggagctc gccgtagcgc tcgatatcac cgatgaacaa ccggtgactt ggtttagccc
    16921 gttagatggc gataataccc tttccgccgc cctgctcgac ttcttcaacg aaatgaatga
    16981 agacggtacg ctggcacgca ttgaagagaa atacctgggg catggcgatg attttgatta
    17041 cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta ctgccgcagt taaagcccct
    17101 gtttgagaaa tacgccgaag atattgactg gcgtttgctg gccgctattg cttatcagga
    17161 atcgcactgg gatgcacagg ccacttcacc gacgggtgtg cgcggcatga tgatgttaac
    17221 caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc gatgccgaac agagcatcag
    17281 cggtggcgtg cgttatttgc aggatatgat gagtaaagtg ccggaaagtg tgccggagaa
    17341 cgagcggatc tggtttgccc tcgctgcgta caatatgggc tatgcgcata tgctggatgc
    17401 ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt tgggctgacg taaaacagcg
    17461 tctgccttta cttagccaga aaccctatta cagcaagctg acttacggct acgctcgtgg
    17521 acatgaagcc tacgcttatg tcgaaaatat tcgtaagtat caaattagcc tggtgggtta
    17581 tctgcaagag aaagagaagc aggctacaga agcggcgatg caactggcgc aggattatcc
    17641 ggcggtatcg cctacggagt tgggcaaaga gaaatttcct tttctctcgt ttctttccca
    17701 gtcgtcatca aactatttga cccattctcc ctctctgctg ttttccagga aagggagtga
    17761 agagaaacaa aattaatccg tcgaggattg cgcttttttc tgcgctttaa tttcctggcg
    17821 gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca tccgccagta ttccttccgt
    17881 aatttccact cggtgattca tacccggatg atgcagcaca tccattaaag atcccgcagc
    17941 gccagttttc gcgtcacgcg caccaaagac cacgcgacca atgcgactgt ggatcatcgc
    18001 tccggcacac attacacatg gttcaagcgt gacatacaac gtggcgtcga tcagacgata
    18061 attttgcatc accagaccac cctgccgcag ggccatgatt tctgcatgtg cggtgggatc
    18121 atggcgacca atcgggcggt tccagccttc gccgattacc cgattgttat gcactaatac
    18181 cgcgccgacc ggcacttccc gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg
    18241 catccagtat tcgtggctaa attcgacttc agacaaaaag aaaactccgg ttataaaagc
    18301 gcggcgcatt atacacggac gctatgcttt actctattcc agttgctgga gttcaccgcg
    18361 cggggttact cgccaacgat gctgacagaa ataaagcagc ggattgtcct gattactgtc
    18421 gctatagcca ctgtacagcc gcagcggagt gccgattttg cgctccagtt gtgcgacctt
    18481 ttcatgtccc agacaacgca tcgtcaatac ccaaccacca tagccacgct gaatttggct
    18541 ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg
    18601 ctgcggagag ccggtaatca accagatatc agcatcggaa cttaacaggt aggtggttaa
    18661 tcgctcctga accagcggaa aggcggtaac attgtcgcga aaccagcgca cgaaatcggc
    18721 ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg cacccccaca gaagcagact
    18781 catcggccag cgtgccgcac gaccttttat caataacgca atggctataa tcggtaacaa
    18841 cggcaggaca agtaacgcat tcagcggttg gcgacgtagt aaatagcgca gaaaactgcc
    18901 gaacatatcc tgctgatgca atgttccatc taagtcaaaa aacaccacac gacgctcgtg
    18961 agttgccaaa ccatactcct ctggactgaa acatcctgat taattttcgc tactacatag
    19021 cctaacagat agatcatcac ttttccggca acaatcgacc gcatcatggc taactggaat
    19081 ttttaattca tggcaattag cggcaatgga atataaaatt cactcgcgtg tgcctcatat
    19141 tgcctgttgt cgccactatt tcatcgcgag gaaagatatg aacggcttac ttcgtatccg
    19201 tcagcgttac caggggcttg cccagagtga taaaaaactg gcggattatc tgctgctaca
    19261 acctgatacg gcgcgccatt taagctctca gcaactggcc aacgaagccg gagtcagtca
    19321 gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa ggttttccgg cgcttaagtt
    19381 ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc tccgtgccca ttcataacca
    19441 aatccgcggt gatgatccgt tacggctggt cggcgaaaaa ctgattaaag aaaatactgc
    19501 cgctatgtac gcaacgctaa acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat
    19561 gttgcgctct gcgcggcgga taattctgac cggtattggc gcttcgggtc tggtggcgca
    19621 aaactttgcc tggaagctga tgaagattgg cttcaatgct gccgcagtgc gcgatatgca
    19681 tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat ctgttattag ccatttccta
    19741 caccggtgta cgacgcgagt taaacctggc ggcagatgag atgctgcgag tgggcggaaa
    19801 agtgctggcg attaccggct ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct
    19861 gtataccatt gccgaagaac aagcgacaaa cagtgcttca atctctgctt gtcacgctca
    19921 gggaatgtta acggatttgc tgttcattgc gctgattcag caggatctgg aactggcacc
    19981 agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg gtctgagtaa ataatgcgcg
    20041 tataatgccc gcccggtttg tgttgttttg agagtttcct tatggcgttg ttaatcacta
    20101 aaaaatgcat caattgtgat atgtgtgaac ccgaatgccc gaatgaggcg atttcaatgg
    20161 gagatcatat ctacgagatt aacagcgata agtgtaccga atgcgtaggg cactacgaga
    20221 caccaacctg ccagaaggtg tgcccgatcc ccaatactat tgtgaaagat ccggcgcatg
    20281 tcgagacaga agaacagttg tgggataaat ttgtgctgat gcaccacgcg gataaaattt
    20341 aatattctac tctggaagta gagtattaat tatattactg ggaagcctta acgccattat
    20401 atttatttaa ttgatgacat tagcataatc attcactaag ttaatttata tagtatctgc
    20461 caagacactt atttatagtt attaaaggcg cgtccgattg gttcaccgga cgcaccttaa
    20521 gtacgtttcc ttgtgttata agaacagaag gatcagctgt aaaacagcaa tgatgatttt
    20581 gatgacccgt ttaatcaggt atcggcaatc agtcattcgt ttttccttaa acaaggaatg
    20641 cagccgttca gattagccct tacatctccc caaaactgaa cgtgcgagtt attgagggtg
    20701 catgctgcac tccacaccag agctttgacg acaccactcg tttcaatggg ggaattctgt
    20761 ggcatggtgt aaagcacagc aaaatcttca ataacgaagc caattttaat gtacttacga
    20821 attggcggtc acgttaatct ttccatcaat attgctttct tcgtaaaggc tcgagttttt
    20881 atgctaaaga ttgcaaattg cttgtaaaag ataagtacac tgatccataa tcgctgttgt
    20941 tgagggtgca tgctgcacaa aattaaagtt aaaaagtaaa acccccgttc cttaccagtt
    21001 cgggggtttt actttttaaa gagaacggta ttattttaac tttcaataat taccgtggca
    21061 caagcatagt ggcgttcatc tgccagcgtt acatgcatat ttacaacgcc gagcttttcc
    21121 gccagtttta atgcctcacc ccatagccgt agccgtggtt tgccgagctc atcattgaat
    21181 acttcaaatt gattaaacgc cagaccattg cggatcccgg tgccaaacgc ttttgctgcg
    21241 gcttctttca cagcaaaacg cttcgccaga aaacgcaccg gctggtggtg cgttttccag
    21301 attgcccatt cgttatcgct taatacgcgg cgtgccaggc gctcaccgga tcgggcgatc
    21361 actgcttcta tgcgagcgat ctctacgata tccatgccta aacctaatat tgccattagc
    21421 cacgcgcttc cagcatcaga cgcttcattt ctgccaccgc atctttcagt ccggtcatca
    21481 ctgcacggcc aataatggca tgaccgatat tcagttcatg catctcaggg atggcggcaa
    21541 tggctttcac gttgtgatag gtcagaccgt gtccggcgtt aactttcaga ccgagactag
    21601 cggcaaaggt cgcggctttg gcgatacgca ccagctcttg cgcctgttcg gcgtcagttt
    21661 tggcatcagc atagcaaccg gtgtggatct cgataaacgg tgcgccaacc tctgccgcag
    21721 ctttgatctg ctcttcatcg gcgtcgataa agagagaaac ctgaatcccg gcatctgtca
    21781 gacgtttgca ggcatcgcgc attttttcac gctgccctgc aacgtccagg ccgccttcgg
    21841 ttgttacttc ctgacgcttt tccggtacca ggcagcaaaa atgtggcttc gtctcaacgg
    21901 cgatcgccag catctcttcg gtcactgcca tctccagatt cattcgggta tccagcgtct
    21961 gccgcaggat gcgcacatcg cggtcagtaa tgtggcggcg atcttcacgt aaatgcactg
    22021 taatgccgtc cgctcccgcc tgctcggcaa taaacgccgc ctgcaccgga tccgggtaag
    22081 cagtaccgcg tgcgttacgc agcgttgcaa tatggtcaat gttgacgcct aacagtaatt
    22141 cagccatgac aatcctcatc attcataatg tgttttcacc gttcgcttag gcataaactg
    22201 ccggaacagt tccctgctct ttaatggttt accgccaaga tacggcttaa gcgccatgcg
    22261 ggtaaagcgt ttcgcggcgc gtagtgtgtc tgcgtcagga aattcccgcg cgtttaacgc
    22321 ttttaactgc cttccggtga acgttttatt gtcgataacg acgcttgcga taaacccttt
    22381 ttcttcgcga taacgatacg tcatggtgtc atctaccggc tcgccgctac ccgcgcaata
    22441 ggtaaaattg acgccatagc ccagatgccc gagcagtgcc agttcaaagc ggcgcagagc
    22501 gggttctggc gtaccagtgg cacctgcaag agactgaatg cagtgcaaat aatcgaaaaa
    22561 gagttcagag aagcgcgtct cgtattccag tacgcgggag agaagttcgt tgatgtacag
    22621 accgctgtaa agcgtgatac tgcttaatgg cagcgccagc gagacagctt cagcactgcg
    22681 cagcgttttg acttcgccac gcccgccgaa acgtagcaag agaggggtga aaggctgtaa
    22741 tgcacctttc agagtagagc gtttagagcg tgcgcctttg gcaaccagac gcacgcgacc
    22801 cgattcctcc gtgaagacgt ccagcatcag gctggtttcg ctccacgggc gactatgcag
    22861 gacaaatgcg cgctgccagc cttccatcgg agtgactctt aaagatcgtc aacgtaaccg
    22921 agactgcgca gtgcgcgttc gtcgtcggcc caaccggatt tcacttttac ccacagttca
    22981 aggtggacag gcgcttcgaa catttcctgc atgtctttac gcgcttcaat cccgatggtt
    23041 ttgatcttgg cccctttgtt gccaataacc atcttcttct gcccttcacg ctcaacgaga
    23101 atcaaaccat tgatgtcata accaccgcgt tcgttagaga cgaaacgttc gatctccacg
    23161 gtcacggagt acggcagttc agcgccgagg aaacgcatca gtttttcgcg gatgatttca
    23221 gacgccataa aacgctgtga gcgatcggtg atgtaatctt ccgggaagtg atgggtcgct
    23281 tcaggcagat gcttacgcac gattgctgcg atagtgtcga cattcagccc ggtttcagca
    23341 gagatcggca cgatatcgag gaagttcatc tgacttgcca ggaactgtaa gtgcggcagc
    23401 agatcggctt tttcctgcac gttatccact ttgttcaccg cgaggatcac cggcgctttg
    23461 ccatcgcgca gtttgttgag caccatttcg tcgtccggcg tccagcgggt gccttcaacg
    23521 acaaaaatca ccagctcaac gtcgccaata gagctgctcg ccgctttgtt catcaggcgg
    23581 ttaatagcgc gtttttcttc catatgcagg ccaggggtat caacgtagat cgcctgatac
    23641 gcgccttcag tatggatccc cacaatgcgg tgacgagttg tttgcgcttt gcgggaggtg
    23701 atggagatct tctgccccag cagtttgttc aacaatgtgg atttgccaac gttcggacgt
    23761 ccgacgatgg caataaatcc gcagtaactt ttatcgatgc tcattccagc tccagttttt
    23821 tcaacgcctg ttcggcggca gcctgctcag ccttacgacg gcttgaacct gtgccaacca
    23881 ccggttcact caggccgctg acctggcagt ggatagtaaa ttcctgatcg tgcgcttcgc
    23941 cacgtacctg gactaccaga taagtcggca gcggcagatg gcgaccctgc aaatattctt
    24001 gcaagcgcgt tttcggatct ttttgtttat cgcctgggct aatttcgtcc aaacgagttt
    24061 gataccagtt gaggattaat ttctcgacgg tttgaatatc actgtcgagg aatacgccac
    24121 caattaatgc ttcgacggta tcggcgagaa ttgactcacg acgaaatcca ccgcttttaa
    24181 gttcacctgg ccctaaacgt aagcactcgc ctaactcaaa ttcgcgcgcc agttccgcca
    24241 gcgtattgcc acggaccagc gtggcgcgca tccggctcat atcgccttca tccacacaag
    24301 ggaaacggtg ataaagcgca ttggcgataa cgtagctcag aatagagtcg cctaaaaatt
    24361 ctaaacgctc gttatgtttg ctgctggcac tacgatgagt taatgcctgc tgcaacagtt
    24421 cctgatgatt aaaagtgtag cccagcttcc gttgaagccg attaattacg atggggttca
    24481 tgcgatacca ataaataaat gcgtcaacaa ttcagcacac gaaacagacc tgatatacat
    24541 ggctctgcta actgcttcgc tgcagtttca gtttaccggt atatggggac caacgctgtt
    24601 tcgtgtgccg tggcaacctg gaggtgccaa ccttaaactt cgggggaata ttctatacac
    24661 aacgacgggg gatgtcgtta gccacgggag atttatctca taaataattc acgttgtcgc
    24721 cataacggcg acaacgtgaa cgaagatggg ctattaatgg atgccgccaa tgcgacttaa
    24781 gcgcacacct gtcggccatt cgccttcttg cttatcgaaa ctcatccaga tagccgttgc
    24841 ccgaccgacc agattcgctt caggcacaaa accccagtaa cggctgtccg cgctgttgtc
    24901 gcggttgtcg cccatcatga agtattgtcc cggcggaaca atccaggttg ccagttgttg
    24961 ccctggctgc tggtaataca tccccacctg gtcctgcgca atcggcactg tcagaattcg
    25021 gtgcgtcaca tcacccagtg tctctttacg ctcggaaaga cgaattccat tttctttggt
    25081 ttcgtttttc ggcacttcaa agaatccgct ggtcgcttcc ccaccattac ggcgtgagaa
    25141 ggtctgaacg aaatcgctcg gttccacgtt ggagtaggtg accggtagcg cgttttcaca
    25201 cgcctggccg gaactgcatc ccggttgaat cgtcagctct tttgagaccg gatcgtaagt
    25261 gactttatcg cccggtaaac ccaccgcgcg cttgatgtaa tcaagctttg gatcttccgg
    25321 atatttaaag accacgatat cgccgcgttt cggatgaccg gtttcgatca gcgttttctg
    25381 gtagatagga tctttaatgc cataagcaaa cttctctacc agaataaaat caccgattaa
    25441 cagagtcggc atcatcgaac ctgacgggat ctggaacggt tcataaataa acgaacgtac
    25501 aatcaatacg atagccagca ccggaaaaac agaagcaccg gtttccagcc agccaggctt
    25561 cggcgcaacc tttttcaggg ttgctttatc cagtgagtca ccggcagccg cctgcgccgc
    25621 tgcctgacgt tcccgccgtt taggtgcgaa aaagaattta tccacgcacc ataaaatgcc
    25681 cgtcaccagt gtggcaatca ccagaatcag ggcaaacata ttcgccatgc caactcctaa
    25741 gggttatttg ttgtctttgc cgacgtgcag aatggcgagg aacgcttcct gcggcagctc
    25801 gacgttaccg atctgcttca tgcgtttctt accttctttc tgcttctgca gcagcttttt
    25861 cttacggctg atatcgccgc cataacattt agccagtacg tttttacgca gctgtttcac
    25921 ggtggatcgc gcaataatgt gcgtaccaat cgctgcctga atggcgatat caaactgctg
    25981 gcgtgggatc agatctttca tcttctccac caactcgcga ccgcggtttt gcgaattatc
    26041 acggtgggtg atcagcgcca acgcatcaac acgttcaccg ttgattaata cgtctacacg
    26101 taccatgtcg gacgcctgga agcgtttgaa gttgtaatcc agagacgcat aaccacgcga
    26161 ggtagatttc aggcgatcga agaagtcgag caccacttcc gccatcggga tctcgtacgt
    26221 cagcgccacc tgattaccgt ggtaaaccat attggtctgc acgccgcgtt tttctacgca
    26281 cagcgtaata acgttgccga gatatgcctg cggcagcagc atgtgacact ctgcaatcgg
    26341 ctcgcgcagt tcgtaaatgt tatttaccgc aggcagcttg gatgggctgt cgacgtagat
    26401 aacttccctt gacgtggttt caacttcata cactacggtc ggcgcagtgg tgatcagatc
    26461 cagatcgtat tcacgttcca gacgttcctg gatgatctcc atgtgcagca ggccaaggaa
    26521 gccgcagcgg aaaccaaagc ccagcgcgct ggagctttcc ggctcataga acagtgaggc
    26581 atcattcagg ctgagtttac ccagcgcgtc acggaaggct tcatagtcgt cggaacttac
    26641 cgggaacaga ccggcgtata cctgcggttt gactttctta aagccaggca gcgccttttc
    26701 tgccggatta cgcgccagcg ttaaggtatc gccgactgga gcgccgtgga tatctttaat
    26761 cgcacatacc agccagccta cttcgccaca tttcagttca gtgcggtcaa cctgtttcgg
    26821 cgtgaagatg cccagacggt cggcgttata ggtctgcccg gtactcatga ctttcacttt
    26881 gtcgccctta cgcagggtgc cgtttttaat acggataagt gaaacaacgc ccaggtagtt
    26941 gtcgaaccat gagtcgataa ttagtgcctg caacgggcct tccggatcgc cttccggcgg
    27001 cggaatgtcg cgcaccagac gttcgagaac gtcctgcacg ccaacgccgg ttttcgctga
    27061 acagcgcacc gcgtcagtgg cgtcgatgcc gacgatatct tcaatttctt ccgccacgcg
    27121 ttcaggatcg gctgccggca ggtcaatctt gttcagtact ggcaccactt cgagatccat
    27181 ttccatggcg gtgtagcagt ttgccagggt ttgcgcttcc acgccctgcc cggcgtcgac
    27241 caccagcaat gcaccttcac aggcagccag cgaacgggaa acttcatagg agaagtctac
    27301 gtggcccggg gtgtcgataa agttaagctg ataggtttcg ccgtcagacg ctttgtagtc
    27361 cagcgtcacg ctttgcgctt tgatggtaat gccacgctca cgctcaagat ccatggaatc
    27421 gagaacctgc gcctccattt cacggtcaga caggccaccg cagatctgga taatacggtc
    27481 agacagcgtc gatttaccgt ggtcaatgtg agctatgatc gaaaagttac gtatattctt
    27541 cataaagtat gattattgtg ccttaatgcc cggttaacca ggcttttaga agtcgctgtt
    27601 ctgagcttaa cgtctgtatt aatagaaacg ccgcattcta cactacaaca ttgaggcgag
    27661 gaaatgttca taccgtatgg attgtggtat ctggaaacgt cctcgcattt gttatgcaaa
    27721 atgcaacaaa gccagtgaaa tcactggctc gcgtcttccg aagatgtttc aaatcgcaca
    27781 aggccaggcg gcaatgccac gcttaagatg atcggttgcc attctgcccg ggcagcaaac
    27841 ttacgcgagt agccgcgcgc aatcaggaac ccgccaatac cgccgaggac cgcaccacat
    27901 aatgctgcga cgtcagaagc aaagagtagc tgaaatagcg aagcgatgag gaataatccc
    27961 accagcggcg acatataaac cagtaatgcg gagctaagca ggctgccttc ggcgatcccc
    28021 aattccactt tttgccccgg cactaacggt tcatcacagg gtacgacaat ggtatgcgtg
    28081 gtttgcgggc caagtttatt caacacgcgg ctaccgcaac cggcgcgtga agcgcagctg
    28141 ctgcatgaag ctttaacatc acaactgacc agcgcctgcc cgttttgcca ggagacgacg
    28201 gtagcccact ctttgatcat tgcgctgccc cgaacttaat attctcggca atgcgtttcg
    28261 ccgtttgcgg cggcagttca ccgacaatgg tgatttcggc gttatcacgt acgcttgtac
    28321 tgacggttct gcgtccggtg cgcaacatct gatcggtgct cgatggcgta gcgcggttaa
    28381 cgtttaccga gaagctgaat aatccgtcag aatagagacg tgattcgata ggcatgttgt
    28441 ccatcgtcgg tagcggacgt cgactactgg aaacttcgct aaaaccctgt ggcaaccagg
    28501 ttggcgtcca gctgaattta gctttttcac ctacaggaac agaaagcaac ggcggcaaat
    28561 ttgccttcgc cagcgtctgc atactgctgc tgatatcctg attgacgtta aaagcaatca
    28621 cgcgaaattg ttccagcgtt tcaccatcgc gatcaaggag atcaacccgc atcggtaatt
    28681 tcgattcggt gtccatccac acgatgtagc tgtagcgtgt accatctcgg gcaaccacgc
    28741 gaatgacttc gcaaagacga tcagcaatac gcgtgcgccc gacggagata aagtcgtagt
    28801 aaggagaaag gcgtttgaaa tcggtataga tgagcgatgg cagagaatca acaatgtaat
    28861 cgccattaag cgtgaacggt tcaagtcccg gttcaaaata gctgatttca tcgccgcgct
    28921 gtaccacttc cctgcgcggg ccatccattt gcaacaattg tgcaagagga cggttatcga
    28981 ggcgtgcatg tcgataacgc agagactcaa caccctgttt attgatgctg atgaatgaca
    29041 gctcgtaatt cagtgactga ctggccaggt tcatctgctg taataacgcc ccggacgcgg
    29101 gagtggccga ggcgttagca gagaataaca ggctacctgt cactaatgac atggcaaacc
    29161 aaagttgctt cattactgcg attgcgttcc taaagtttga attcctggca cctgtacagc
    29221 ggcttgctgg gtttgcgcct gctcaaactg aagctgttca gagtggagtc ggcgttgcag
    29281 ttcgtaatcc tgcaacattg cattaatgcg acgacgctgc tcctgtacct gctgctgttg
    29341 accattgttt gcggtcgctt cagaaggtac tcccaggctt accgggctgg ctttacccat
    29401 catcggcagt gtattaaata ccggcgtttc gggctgctgg gacgtttcag attgtccatt
    29461 atagtgctgg acgccaacga taactgcaag cgatacgcat gctgctacgc ccatttgggt
    29521 aagctgtgcc gcccacggac gtactttctg ccagaatggc attttctgcc attgatgcgg
    29581 cgcaggctgg gcttccggga tcaatgtcgc cggttgacgt actggctctt cttcaatggc
    29641 ggccatcacg cgtgaagaga tatcgaaatg gagcacctcg ggagtatcac cccgcattga
    29701 gtcacggatt aagtgatagc tttcccaggt tttctgcatt tctgggttat gagccagttc
    29761 gttaagcagc tcactatcca gcgtttcgcc atccattaaa gcggaaagtt gttctttctg
    29821 catgcctaat acccttatcc agtatcccgc tatcgtcaac gcctgataag cggttgaact
    29881 ttgttatcaa tagcttccct cgctcggaag atacgtgaac gcaccgtacc taccggacaa
    29941 tccatgatag cggctatctc ttcatagctc aggccatcca gctcccgcaa ggttattgcc
    30001 atgcgcaaat cttccgggag ggactcaata gttcggaaaa ctatctgtct cagttcttct
    30061 gacaacatta agttctcagg gttcgaaatt tctttcaacg cgccgccact ttcgaagttt
    30121 tcagcttcaa tggcatccac atcactggaa ggtggacgac gcccctgagc aaccaggtaa
    30181 tttttcgctg tatttacagc aatccgatac agccatgtat aaaaagcgct atctccccgg
    30241 aacgaatcca gcgcacgata ggctttaata aaagcttctt gtaccacatc gggaacatca
    30301 cccgacggca catagcggga aaccagactc gccactttat gctgatagcg cactaccagt
    30361 aagttaaagg ctttctgatc tcccttctgg acccgttcaa ccaggacctg gtccgttaac
    30421 tgctcgctca tccgaggtaa agtctcccca aaccaaattt ccacgcgcta tcgaaacgcc
    30481 actccattag ctgcaatttg agcaagcaaa gggttagagt gtctcgtttt tgtaaagttc
    30541 cgtaacgcat ctgtttttgt ttgtcatgct gtagacggat cattatctat cattataagt
    30601 ctacagaatc tgaacatcgc attatctgtg tagaaatgcc catttaactg cctgaagagt
    30661 aacccaacgg cctttttatt tcaccaccta atcctccacc agccagtaac ttctcttttt
    30721 ctcgccgccc tgcatcagcg tgtttagcaa ctgtaacaaa tattaaaata gcaggtgttt
    30781 attcgcacaa catgatgcta tgctgaccaa accgtgttta gtaaattaga caaagaaaat
    30841 gaatactctc cctgaacatt catgtgacgt gttgattatt ggtagcggcg cagccggact
    30901 ttcactggcg ctacgcctgg ctgaccagca tcaggtcatc gttctaagta aaggcccggt
    30961 aacggaaggt tcaacatttt atgcccaggg cggtattgcc gccgtgtttg atgaaactga
    31021 cagcattgac tcgcatgtgg aagacacatt gattgccggg gctggtattt gcgatcgcca
    31081 tgcagttgaa tttgtcgcca gcaatgctcg ttcctgtgtg caatggttaa ttgaccaggg
    31141 ggttttgttt gatacccacg ttcaaccgaa tggcgaagaa agctaccatc tgacccgtga
    31201 aggtggacat agtcaccgtc gtattcttca tgccgctgac gccaccggta gagaagtaca
    31261 atccacgctg gtgagcaagg cgcagaacca tccgaatatt cgtgtgctgg agcgcagcaa
    31321 cgcagttgat ctgattgtct ctgacaaaat tggcctgccg ggcacgcgac
//
LOCUS       DAJTMK010000029        30930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTMK010000029 DAJTMK010000000
VERSION     DAJTMK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30930
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.030"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..75
                     /locus_tag="OO135_001142"
     CDS             <1..75
                     /locus_tag="OO135_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397070.1"
                     /translation="DGLRFAIREGGRTVGAGVVAKVLS"
     gene            305..688
                     /gene="secE"
                     /locus_tag="OO135_001143"
     CDS             305..688
                     /gene="secE"
                     /locus_tag="OO135_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5397071.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            690..1235
                     /gene="nusG"
                     /locus_tag="OO135_001144"
     CDS             690..1235
                     /gene="nusG"
                     /locus_tag="OO135_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5397072.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1395..1823
                     /gene="rplK"
                     /locus_tag="OO135_001145"
     CDS             1395..1823
                     /gene="rplK"
                     /locus_tag="OO135_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5397073.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1827..2531
                     /gene="rplA"
                     /locus_tag="OO135_001146"
     CDS             1827..2531
                     /gene="rplA"
                     /locus_tag="OO135_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5397074.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2944..3441
                     /gene="rplJ"
                     /locus_tag="OO135_001147"
     CDS             2944..3441
                     /gene="rplJ"
                     /locus_tag="OO135_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5397075.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3508..3873
                     /gene="rplL"
                     /locus_tag="OO135_001148"
     CDS             3508..3873
                     /gene="rplL"
                     /locus_tag="OO135_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5397076.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4193..8221
                     /gene="rpoB"
                     /locus_tag="OO135_001149"
     CDS             4193..8221
                     /gene="rpoB"
                     /locus_tag="OO135_001149"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5397077.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8298..12521
                     /gene="rpoC"
                     /locus_tag="OO135_001150"
     CDS             8298..12521
                     /gene="rpoC"
                     /locus_tag="OO135_001150"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5397078.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12734..13273
                     /locus_tag="OO135_001151"
     CDS             12734..13273
                     /locus_tag="OO135_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HCR5397079.1"
                     /translation="MKQEVEKWRPFGDPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13683..14816)
                     /gene="thiH"
                     /locus_tag="OO135_001152"
                     /pseudo
     CDS             complement(13683..14816)
                     /gene="thiH"
                     /locus_tag="OO135_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
     gene            complement(14813..15583)
                     /gene="thiG"
                     /locus_tag="OO135_001153"
     CDS             complement(14813..15583)
                     /gene="thiG"
                     /locus_tag="OO135_001153"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5397080.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15585..15785)
                     /gene="thiS"
                     /locus_tag="OO135_001154"
     CDS             complement(15585..15785)
                     /gene="thiS"
                     /locus_tag="OO135_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5397081.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15769..16524)
                     /gene="thiF"
                     /locus_tag="OO135_001155"
                     /pseudo
     CDS             complement(15769..16524)
                     /gene="thiF"
                     /locus_tag="OO135_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16517..17152)
                     /gene="thiE"
                     /locus_tag="OO135_001156"
     CDS             complement(16517..17152)
                     /gene="thiE"
                     /locus_tag="OO135_001156"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5397082.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17152..19047)
                     /gene="thiC"
                     /locus_tag="OO135_001157"
     CDS             complement(17152..19047)
                     /gene="thiC"
                     /locus_tag="OO135_001157"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5397083.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQLQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSIKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19128..19228)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19281..19757)
                     /gene="rsd"
                     /locus_tag="OO135_001158"
     CDS             complement(19281..19757)
                     /gene="rsd"
                     /locus_tag="OO135_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5397084.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19852..20625
                     /gene="nudC"
                     /locus_tag="OO135_001159"
     CDS             19852..20625
                     /gene="nudC"
                     /locus_tag="OO135_001159"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5397085.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20665..21729
                     /gene="hemE"
                     /locus_tag="OO135_001160"
     CDS             20665..21729
                     /gene="hemE"
                     /locus_tag="OO135_001160"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5397086.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21739..22410
                     /gene="nfi"
                     /locus_tag="OO135_001161"
     CDS             21739..22410
                     /gene="nfi"
                     /locus_tag="OO135_001161"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5397087.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22453..23043
                     /locus_tag="OO135_001162"
     CDS             22453..23043
                     /locus_tag="OO135_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5397088.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23230..23502
                     /gene="hupA"
                     /locus_tag="OO135_001163"
     CDS             23230..23502
                     /gene="hupA"
                     /locus_tag="OO135_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5397089.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23515..24210
                     /locus_tag="OO135_001164"
     CDS             23515..24210
                     /locus_tag="OO135_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5397090.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24212..24637)
                     /gene="zraP"
                     /locus_tag="OO135_001165"
     CDS             complement(24212..24637)
                     /gene="zraP"
                     /locus_tag="OO135_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5397091.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24875..26251
                     /gene="zraS"
                     /locus_tag="OO135_001166"
     CDS             24875..26251
                     /gene="zraS"
                     /locus_tag="OO135_001166"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5397092.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26248..27572
                     /gene="zraR"
                     /locus_tag="OO135_001167"
                     /pseudo
     CDS             26248..27572
                     /gene="zraR"
                     /locus_tag="OO135_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27569..28858)
                     /gene="purD"
                     /locus_tag="OO135_001168"
     CDS             complement(27569..28858)
                     /gene="purD"
                     /locus_tag="OO135_001168"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5397093.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28870..30459)
                     /gene="purH"
                     /locus_tag="OO135_001169"
     CDS             complement(28870..30459)
                     /gene="purH"
                     /locus_tag="OO135_001169"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5397094.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7317 a         7990 c         8447 g         7176 t
ORIGIN      
        1 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgtagca
       61 aaagttctga gctaattgcc gataacattt gacgcaatgc gcactaaaag ggcatcattt
      121 gatgcccttt ttgcacgctt tcgtaccaga acctggctca tcagtgattt tctttgtcat
      181 aatcattgct gagacaggct ctgttgaggg cgtataatcc gaaaagctaa tacgcgtttc
      241 gatttggttt gcctcgcgat ctcggggtga aaatgtttgt agaaaacttc tgacaggttg
      301 gtttatgagt gcgaataccg aagctcaagg aagcgggcgc ggcctggaag cgatgaagtg
      361 ggtcgttgtg gtggcattgc tcttggtggc gattgtcggc aactaccttt atcgcgacat
      421 tatgctgccg ctgcgtgcgc tggccgtagt aattctgatt gctgcagcgg gtggtgtcgc
      481 gctgttaacg acaaaaggta aagctaccgt tgcttttgcc cgtgaagcgc gtaccgaagt
      541 ccgtaaggtc atttggccga ctcgccagga aacattgcac accacgctga ttgtggctgc
      601 ggttaccgca gtaatgtcac tgatcctgtg gggactggat ggtattctgg ttcgcctggt
      661 atcctttatc actggcctga ggttctgaga tgtctgaagc tcctaaaaag cgctggtacg
      721 tcgttcaggc gttttccggt tttgaaggcc gcgtagcaac gtcgctgcgt gagcatatca
      781 aattacacaa catggaagat ttgtttggtg aagtcatggt accaaccgaa gaagtggttg
      841 aaatccgtgg cggtcagcgt cgcaaaagcg aacgtaaatt cttccctggc tacgtcctcg
      901 ttcagatggt gatgaacgac gcgagctggc acctggtgcg cagcgtaccg cgagtgatgg
      961 gcttcatcgg cggtacttcc gatcgtcctg cgccaatcag cgataaagaa gtcgatgcga
     1021 ttatgaaccg cctgcagcag gttggtgata agccgcgtcc gaaaacgctg tttgaaccgg
     1081 gtgaaatggt ccgtgttaat gatggtccgt tcgctgactt caacggtgtt gttgaagaag
     1141 tggattacga gaaatctcgt ctgaaagtgt ctgtttctat cttcggtcgt gcgaccccgg
     1201 tagagctgga cttcagccag gttgaaaaag cctaacccag cgatcaaaaa agcggcgatt
     1261 taatcgttgc acaaggcgcg agattggaat acaatttcgc gccttttgtt tttatgggcc
     1321 ttgcccgtaa aaacgatttt ttatatcacg gggagcctct cagaggcgtt attacccaac
     1381 ttgaggaatt tataatggct aagaaagtac aagcctatgt caagctgcag gttgcagctg
     1441 gtatggctaa cccgagtccg ccagtaggtc cggctctggg tcagcagggc gtaaacatca
     1501 tggaattctg caaagcgttc aacgcaaaaa ctgattccat cgaaaaaggt ctgccgattc
     1561 cggtagtaat caccgtttac gctgaccgtt ctttcacttt cgttaccaag accccgccgg
     1621 cagcagttct gctgaaaaaa gcggctggta tcaagtctgg ttccggtaag ccgaacaaag
     1681 acaaagtggg taaaatttcc cgcgctcagc tgcaggaaat cgcgcagacc aaagctgccg
     1741 acatgactgg tgccgacatt gaagcgatga ctcgctccat cgaaggtact gcacgttcca
     1801 tgggcctggt agtggaggac taagaaatgg ctaaactgac caagcgcatg cgtgttatcc
     1861 gcgagaaagt tgatgcaacc aaacagtacg acatcaacga agctatcgca ctgctgaaag
     1921 agctggcgac tgctaaattc gtagaaagcg tggacgtagc tgttaacctc ggcatcgacg
     1981 ctcgtaaatc tgaccagaac gtacgtggtg caactgtact gccgcacggt actggccgtt
     2041 ccgttcgcgt agccgtattt acccaaggtg caaacgctga agctgctaaa gctgcaggcg
     2101 cagaactggt aggtatggaa gatctggctg accagatcaa gaaaggcgaa atgaactttg
     2161 acgttgttat tgcttctccg gatgcaatgc gcgttgttgg ccagctgggc caggttctgg
     2221 gtccgcgcgg cctgatgcca aacccgaaag tgggtactgt aacaccgaac gttgctgaag
     2281 cggttaaaaa cgctaaagct ggccaggttc gttaccgtaa cgacaaaaac ggcatcatcc
     2341 acaccaccat cggtaaagtg gactttgacg ctgacaaact gaaagaaaac ctggaagctc
     2401 tgctggttgc gctgaaaaaa gcaaaaccga ctcaggcgaa aggcgtgtac atcaagaaag
     2461 ttagcatctc caccaccatg ggtgcaggtg ttgcagttga ccaggctggc ctgagcgctt
     2521 ctgtaaacta atgcctttac gtgggcggtg attttgtcta caatcttacc cccacgtata
     2581 atgcttaatg cagacgtata tccgagatat tcgggttgtg gcaaggcggc aactgagtga
     2641 gtcgccagga gcatagctaa ctatgtgact ggtgcgaatg aaggaagcca acgccgccac
     2701 aagttgaata gcgacggata gaaaagattt gttcgttgga gcctggccta tccaggcctc
     2761 cgtcgaagac cgcaggagtt tcgcaagaaa cttaatcccc tgcgtagacg gtgacagaac
     2821 gctaagatta ttcttttata ttctggcttg tttctgctca ccgtaattaa gacgctctct
     2881 ccgtttggag gagtgaagtg agttccagag attttctctg gcaaacatcc aggagcaaag
     2941 ctaatggctt taaatcttca agacaaacaa gcgattgttg ctgaagtcag cgaagtagcc
     3001 aaaggcgcgc tgtctgcagt agttgcggat tcccgtggcg taactgtaga taaaatgact
     3061 gaactgcgta aagcaggtcg cgaagctggc gtatacatgc gtgttgttcg taacactctg
     3121 ctgcgccgtg ctgttgaagg tactccgttc gagtgcctga aagacgcgtt tgttggtccg
     3181 accctgattg catactctat ggaacacccg ggcgctgctg ctcgtctgtt caaagagttc
     3241 gcgaaagcga atgcaaaatt tgaggtcaaa gccgctgcct ttgaaggtga gctgatcccg
     3301 gcgtctcaga tcgaccgcct ggcaactctg ccgacctacg aagaagcaat tgcacgcctg
     3361 atggcaacca tgaaagaagc ttcggctggc aaactggttc gtactctggc tgctgtacgc
     3421 gatgcgaaag aagctgctta atcgcagtta tctttttaac gcattcgctt acgtataaac
     3481 ttattctgat attcaggaac aatttaaatg tctatcacta aagatcaaat cattgaagca
     3541 gttgcagcta tgtctgtaat ggacgttgta gaactgatct ctgcaatgga agaaaaattc
     3601 ggtgtttccg ctgctgctgc tgtagctgta gctgctggcc cggttgaagc tgctgaagaa
     3661 aaaactgaat tcgacgtaat tctgaaagct gctggcgcta acaaagttgc tgttatcaaa
     3721 gcagtacgtg gcgcaactgg cctgggtctg aaagaagcta aagacctggt agaatctgca
     3781 ccggctgctc tgaaagaagg cgtgagcaaa gacgacgcag aagcactgaa aaaagctctg
     3841 gaagaagctg gcgctgaagt tgaagttaaa taagccaacc cttccggttg cagcctgaga
     3901 aatcaggctg atggctggtg actttttagt caccagcctt tttgcgctgt aaggcgccag
     3961 tagcgtttca cactgtttga ctactgctgt gcctttcaat gcttgtttct atcgacgact
     4021 taatatactg cgacaggacg tccgttctgt gtaaatcgca atgaaatggt ttaagcgtga
     4081 tagcaacagg cattgcggaa agtgttccat tttccggtca acaaaatagt gttgcacaaa
     4141 ctgtccgctc aatggacaga tgggtcgact tgtcagcgag ctgaggaacc ctatggttta
     4201 ctcctatacc gagaaaaaac gtattcgtaa ggattttggt aaacgtccac aagttctgga
     4261 tgtaccttat ctcctttcta tccagcttga ctcgtttcag aaatttatcg agcaagatcc
     4321 tgaagggcag tatggtctgg aagcagcttt ccgttccgta ttcccgattc agagctacag
     4381 cggtaattcc gagctgcaat acgtcagcta ccgccttggc gaaccggtgt ttgacgtcca
     4441 ggaatgtcaa atccgtggcg tgacctattc cgcaccgctg cgcgttaaac tgcgtctggt
     4501 gatctatgag cgcgaagcgc cggaaggcac cgtaaaagac attaaagaac aagaagtcta
     4561 catgggcgaa attccgctca tgacagacaa cggtaccttt gttatcaacg gtactgagcg
     4621 tgttatcgtt tcccagctgc accgtagtcc gggcgtcttc tttgactccg acaaaggtaa
     4681 aacccactct tcgggtaaag tgctgtataa cgcgcgcatc atcccttacc gtggttcctg
     4741 gctggacttc gaattcgatc cgaaggacaa cctgttcgta cgtatcgacc gtcgccgtaa
     4801 actgcctgcg accatcattc tgcgcgccct gaactacacc acagagcaga tcctcgacct
     4861 gttctttgaa aaagttatct ttgaaatccg tgataacaag ctgcagatgg aactggtgcc
     4921 ggaacgcctg cgtggtgaaa ccgcatcttt tgacatcgaa gctaacggta aagtgtacgt
     4981 agaaaaaggc cgccgtatca ctgcgcgcca cattcgccag ctggaaaaag acgacgtcaa
     5041 acttatcgaa gtcccggttg agtacatcgc aggtaaagtg gttgctaaag actatattga
     5101 tgagtctacc ggcgagctga tctgcgcagc gaacatggag ctgagcctgg atctgctggc
     5161 taagctgagc cagtctggtc acaagcgtat cgaaacgctg ttcaccaacg atctggatca
     5221 cggcccatat atctctgaaa ccttacgtgt cgacccaact aacgaccgtc tgagcgcact
     5281 ggtagaaatc taccgcatga tgcgccctgg cgagccgccg actcgtgaag cagctgaaag
     5341 cctgttcgag aacctgttct tctccgaaga ccgttatgac ttgtctgcgg ttggtcgtat
     5401 gaagttcaac cgttctctgc tgcgcgaaga aatcgaaggt tccggtatcc tgagcaaaga
     5461 cgacatcatt gatgttatga aaaagctcat cgatatccgt aacggtaaag gcgaagtcga
     5521 tgatatcgac cacctcggca accgtcgtat ccgttccgtt ggcgaaatgg cggaaaacca
     5581 gttccgcgtt ggcctggtac gtgtagagcg tgcggtgaaa gagcgtctgt ctctgggcga
     5641 tctggatacc ctgatgcctc aggatatgat caacgccaag ccgatttccg cagcagtgaa
     5701 agagttcttc ggttccagcc agctgtctca gtttatggac cagaacaacc cgctgtctga
     5761 gattacgcac aaacgtcgta tctccgcact cggcccaggc ggtctgaccc gtgaacgtgc
     5821 aggcttcgaa gttcgagacg tacacccgac tcactacggt cgcgtatgtc caatcgaaac
     5881 ccctgaaggt ccgaacatcg gtctgatcaa ctctctgtcc gtgtacgcac agactaacga
     5941 atacggcttc cttgagactc cgtatcgtaa agtgaccgac ggtgttgtaa ctgacgaaat
     6001 tcactacctg tctgctatcg aagaaggcaa ctacgttatc gcccaggcga actccaacct
     6061 ggatgaagaa ggccacttcg tagaagacct ggtaacttgc cgtagcaaag gcgaatccag
     6121 cttgttcagc cgcgaccagg ttgactacat ggacgtatcc acccagcagg tggtatccgt
     6181 cggtgcgtcc ctgatcccgt tcctggaaca cgatgacgcc aaccgtgcat tgatgggtgc
     6241 gaacatgcaa cgtcaggccg ttccgactct gcgcgctgat aagccgctgg ttggtactgg
     6301 tatggaacgt gctgttgccg ttgactccgg tgtaactgcg gtagctaaac gtggtggtgt
     6361 cgttcagtac gtggatgctt cccgtatcgt tatcaaagtt aacgaagacg agatgtatcc
     6421 gggtgaagca ggtatcgaca tctacaacct gaccaaatac acccgttcta accagaacac
     6481 ctgtatcaac cagatgccgt gtgtgtctct gggtgaaccg gttgaacgtg gcgacgtgct
     6541 ggcagacggt ccgtccaccg acctcggtga actggcgctt ggtcagaaca tgcgcgtagc
     6601 gttcatgccg tggaatggtt acaacttcga agactccatc ctcgtatccg agcgtgttgt
     6661 tcaggaagac cgtttcacca ccatccacat tcaggaactg gcgtgtgtgt cccgtgacac
     6721 caagctgggg ccggaagaga tcaccgctga catcccgaac gtgggtgaag ctgcgctctc
     6781 caaactagat gaatccggta tcgtttacat tggtgcggaa gtgaccggtg gcgacattct
     6841 ggttggtaag gtaacgccga aaggtgaaac tcagctgacc ccagaagaaa aactgctgcg
     6901 tgcgatcttc ggtgagaaag cctctgacgt taaagactct tctctgcgcg taccaaacgg
     6961 tgtatccggt acggttatcg acgttcaggt ctttactcgc gatggcgtag aaaaagacaa
     7021 acgtgcgctg gaaatcgaag aaatgcagct caaacaggcg aagaaagacc tgtctgaaga
     7081 actgcagatc ctcgaagcgg gtctgttcag ccgtatccgt gctgtgctgg tagccggtgg
     7141 cgttgaagct gagaagctcg acaaactgcc gcgcgatcgc tggctggagc tgggcctgac
     7201 agacgaagag aaacaaaatc agctggaaca gctggctgag cagtatgacg aactgaaaca
     7261 cgagttcgag aagaaactcg aagcgaaacg ccgcaaaatc acccagggcg acgatctggc
     7321 accgggcgtg ctgaagattg ttaaggtata tctggcggtt aaacgccgta tccagcctgg
     7381 tgacaagatg gcaggtcgtc acggtaacaa gggtgtaatt tctaagatca acccgatcga
     7441 agatatgcct tacgatgaaa acggtacgcc ggtagacatc gtactgaacc cgctgggcgt
     7501 accgtctcgt atgaacatcg gtcagatcct cgaaacccac ctgggtatgg ctgcgaaagg
     7561 tatcggcgac aagatcaacg ccatgctgaa acagcagcaa gaagtcgcga aactgcgcga
     7621 attcatccag cgtgcgtacg atctgggcgc tgacgttcgt cagaaagttg acctgagtac
     7681 cttcagcgat gaagaagtta tgcgtctggc tgaaaacctg cgcaaaggta tgccaatcgc
     7741 aacgccggtg ttcgacggtg cgaaagaagc agaaattaaa gagctgctga aacttggcga
     7801 cctgccgact tccggtcaga tccgcctgta cgatggtcgc actggtgaac agttcgagcg
     7861 tccggtaacc gttggttaca tgtacatgct gaaactgaac cacctggtcg acgacaagat
     7921 gcacgcgcgt tccaccggtt cttacagcct ggttactcag cagccgctgg gtggtaaggc
     7981 acagttcggt ggtcagcgtt tcggggagat ggaagtgtgg gcgctggaag catacggcgc
     8041 agcatacacc ctgcaggaaa tgctcaccgt taagtctgat gacgtgaacg gtcgtaccaa
     8101 gatgtataaa aacatcgtgg acggcaacca tcagatggag ccgggcatgc cagaatcctt
     8161 caacgtattg ttgaaagaga ttcgttcgct gggtatcaac atcgaactgg aagacgagta
     8221 attctcgctc aaacaggtca ctgctgtcgg gttaaaaccc ggcagcggat tgtgctaact
     8281 ccgacgggag caaatccgtg aaagatttat taaagtttct gaaagcgcag actaaaaccg
     8341 aagagtttga tgcgatcaaa attgctctgg cttcgccaga catgatccgt tcatggtctt
     8401 tcggtgaagt taaaaagccg gaaaccatca actaccgtac gttcaaacca gaacgtgacg
     8461 gccttttctg cgcccgtatc tttgggccgg taaaagatta cgagtgcctg tgcggtaagt
     8521 acaagcgcct gaaacaccgt ggcgtcatct gtgagaagtg cggcgttgaa gtgacccaga
     8581 ctaaagtacg ccgtgagcgt atgggccaca tcgaactggc ttccccgact gcgcacatct
     8641 ggttcctgaa atcgctgccg tcccgtatcg gtctgctgct cgatatgccg ctgcgcgata
     8701 tcgaacgcgt actgtacttt gaatcctatg tggttatcga aggcggtatg accaacctgg
     8761 aacgtcagca gatcctgact gaagagcagt atctggacgc gctggaagag ttcggtgacg
     8821 aattcgacgc gaagatgggt gcggaagcaa tccaggctct gctgaagagc atggatctgg
     8881 agcaagagtg cgaacagctg cgtgaagagc tgaacgaaac caactccgaa accaagcgta
     8941 aaaagctgac caagcgtatc aaactgctgg aagcgttcgt tcagtctggt aacaaaccag
     9001 agtggatgat cctgaccgtt ctgccggtac tgccgccaga tctgcgtccg ctggttccgc
     9061 tggatggtgg tcgtttcgcg acttctgacc tgaacgatct gtatcgtcgc gtcattaacc
     9121 gtaacaaccg tctgaaacgt ctgctggatc tggctgcgcc ggacatcatc gtacgtaacg
     9181 aaaaacgtat gctgcaggaa gcggtagacg ccctgctgga taacggtcgt cgcggtcgtg
     9241 cgatcaccgg ttctaacaag cgtcctctga aatctttggc cgacatgatc aaaggtaaac
     9301 agggtcgttt ccgtcagaac ctgctcggta agcgtgttga ctactccggt cgttctgtaa
     9361 tcaccgtagg tccatacctg cgtctgcatc agtgcggtct gccgaagaaa atggcgctgg
     9421 agctgttcaa accgttcatc tacggcaagc tggaactgcg tggtcttgct accaccatta
     9481 aagctgcgaa gaaaatggtt gagcgcgaag aagctgtcgt ttgggatatc ctggacgaag
     9541 ttatccgcga acacccggta ctgctgaacc gtgcaccgac tctgcaccgt ctgggtatcc
     9601 aggcatttga accggtactg atcgaaggta aagctatcca gctgcacccg ctggtttgtg
     9661 cggcatataa cgccgacttc gatggtgacc agatggctgt tcacgtaccg ctgacgctgg
     9721 aagcccagct ggaagcgcgt gcgctgatga tgtctaccaa caacatcctg tccccggcga
     9781 acggcgaacc aatcatcgtt ccgtctcagg acgttgttct gggtctgtac tatatgaccc
     9841 gtgactgtgt taacgccaaa ggcgaaggca tggtgctgac tggcccgaaa gaagcagaac
     9901 gtctgtatcg ctctggtctg gcttctctgc atgcgcgcgt taaagtgcgt atcaccgagt
     9961 atgaaaaaga tgcaaacggt gaattagtag cgaaaaccag cctgaaagac acgactgttg
    10021 gccgtgccat tctgtggatg attgtaccga aaggtctgcc ttactccatc gtcaaccagg
    10081 cgctgggtaa aaaagcaatc tccaaaatgc tgaacacctg ctaccgcatt ctcggtctga
    10141 aaccgaccgt tatttttgcg gaccagatca tgtacaccgg cttcgcctat gcagcgcgtt
    10201 ctggtgcatc tgttggcatc gatgacatgg tcatcccgga gaagaaacac gaaatcatct
    10261 ccgaggcaga agcagaagtt gctgaaattc aggagcagtt ccagtctggt ctggtaactg
    10321 cgggcgaacg ctacaacaaa gttatcgata tctgggctgc ggcgaacgat cgtgtatcca
    10381 aagcgatgat ggataacctg caaactgaaa ccgtgattaa ccgtgacggt caggaagaga
    10441 agcaggtttc cttcaacagc atctacatga tggccgactc cggtgcgcgt ggttctgcgg
    10501 cacagattcg tcagcttgct ggtatgcgtg gtctgatggc gaagccggat ggctccatca
    10561 tcgaaacgcc aatcaccgcg aacttccgtg aaggtctgaa cgtactccag tacttcatct
    10621 ccacccacgg tgctcgtaaa ggtctggcgg ataccgcact gaaaactgca aactccggtt
    10681 acctgactcg tcgtctggtt gacgtggcgc aggacctggt ggttaccgaa gacgattgtg
    10741 gtacccatga aggtatcatg atgactccgg ttatcgaagg tggtgatgtt aaagagccgc
    10801 tgcgcgatcg cgtactgggt cgtgtaactg ctgaagacgt tctgaagccg ggtactgctg
    10861 atatcctcgt tccgcgcaac acgctgctgc acgaacagtg gtgtgacctg ctggaagaga
    10921 actctgtcga cgcggttaaa gtacgttctg ttgtatcttg tgacaccgac tttggtgtat
    10981 gtgcgcactg ctacggtcgt gacctggcgc gtggccacat catcaacaag ggtgaagcaa
    11041 tcggtgttat cgcggcacag tccatcggtg aaccgggtac acagctgacc atgcgtacgt
    11101 tccacatcgg tggtgcggca tctcgtgcgg ctgctgaatc cagcatccag gtgaaaaaca
    11161 aaggtagcat caagctcagc aacgtgaagt cggttgtgaa ctccagcggt aaactggtta
    11221 tcacttcccg taatactgaa ctgaaactga tcgacgaatt cggtcgtacc aaagaaagct
    11281 acaaagtacc ttacggtgcg gtactggcga aaggcgatgg cgaacaggtt gctggcggcg
    11341 aaaccgttgc aaactgggac ccgcacacca tgccggttat caccgaagta agcggttttg
    11401 tacgctttac tgacatgatc gacggtcaga ccattactcg tcagacagac gaattgaccg
    11461 gtctgtcttc gctggtggtt ctggattccg cagaacgtac cgcaggtggt aaagatctgc
    11521 gtccggcact gaaaatcgtt gatgctcagg gtaacgacgt tctgattccg ggtaccgata
    11581 tgcctgcgca gtacttcctg ccgggtaaag cgattgttca gctggaagat ggcgtacaga
    11641 tcagctctgg tgacaccctg gcgcgtattc cgcaggaatc cggcggtacc aaggacatca
    11701 ccggtggtct gccgcgcgtt gcggacctgt tcgaagcacg tcgtccgaaa gagccggcaa
    11761 tcctggctga aatcagcggt atcgtttcct tcggtaaaga aaccaaaggt aaacgtcgtc
    11821 ttgttatcac cccggtagac ggtagcgatc cgtacgaaga gatgattccg aaatggcgtc
    11881 agctcaacgt gttcgaaggt gaacgtgtag aacgtggtga cgtaatttcc gacggtccgg
    11941 aagcgccgca cgacattctg cgtctgcgtg gtgttcatgc tgttactcgt tacatcgtta
    12001 acgaagtaca ggacgtatac cgtctgcagg gcgttaagat taacgataaa cacatcgaag
    12061 ttatcgttcg tcagatgctg cgtaaagcta ccatcgttaa cgcgggcagc tccgacttcc
    12121 tggaaggcga acaggttgaa tactctcgcg tcaagatcgc aaaccgcgaa ctggaagcga
    12181 acggcaaagt gggtgcaact tactcccgcg atctgctggg tatcaccaaa gcgtctctgg
    12241 caaccgagtc cttcatctcc gcggcatcgt tccaggagac cactcgcgtg ctgaccgaag
    12301 cagccgttgc gggcaaacgc gacgaactgc gcggcctgaa agagaatgtt atcgtgggtc
    12361 gtctgatccc ggcaggtacc ggttacgcgt accaccagga tcgtatgcgt cgccgtgctg
    12421 cgggtgaagc tccggctgca ccgcaggtga ctgcagaaga cgcatctgcc agcctggcag
    12481 aactgctgaa cgcaggtctg ggcggttctg ataacgagta atcgttaatc cgcaaataac
    12541 gtaaaaaccc gcttcggcgg gtttttttat ggggggagtt tagggaaaga gcatttgtca
    12601 gaatatttaa ggaatttctg aatactcata atcaatgtag agatgactaa tatcctgaaa
    12661 ctgactgaac taattgagtc aaactcggca aggattcgat actattcctg tgtaactttc
    12721 ttaaggaacg agaatgaaac aggaagtgga aaagtggcga ccttttggag atccggatgg
    12781 tgatattcgt gatttatcat ttcttgatgc tcatcaggct gtctacgttc agcatcatga
    12841 gggcaaagag cctttagagt atcgcttttg ggttacctac tctcttcact gcttcacaaa
    12901 agattatgaa catcagacga acgaagaaaa acaatcgtta atgtaccacg cgcctaaaga
    12961 atctcgtccc ttctgccagc accgttataa cttagcgcgc acacacttaa aaagaactat
    13021 tttggcgctg ccagaaagca acgttattca tgccgggtat ggtagctatg ccgtgattga
    13081 ggtggactta gacggaggag ataaggcatt ttactttgtt gcgttcaggg ctttcaggga
    13141 aaagaaaaaa ctccgtttgc atgtaactag cgcttatccc atttctgaaa aacagaaagg
    13201 taaatcagtg aaatttttca ccattgccta caacttattg agaaataagc agcttcctca
    13261 gccctcaaaa taacaaaacc caccttaagg tgggtttcgc cagagaatta tctctggtat
    13321 tcagaacgcc attaccggac tttgccttga ccttgcgata atcgcaggtt gcgggatgtc
    13381 tgaatttctt cagtctgctg catcctggaa gatgagaaca tgtgttctta ttttcgtctc
    13441 tatcatagtt gagtatttac tctcttacaa tcagatctct ttcattgctc aacaggcgat
    13501 ggcttcagac tttgcattac ggaattttta agaaaggcag ggcgaaacga ggaagaagct
    13561 tttcgccctg gacaacattc ctgctgacgg cactaccata aaaaaacaca acgctttcaa
    13621 caaccttgcg tttttcatct tccgtagcgg ctcggaataa atttacgttc ttacattccg
    13681 tctcatagtc tttgcgaggc ccgtcccaga tagctgtccc agtctttcca taccggctgc
    13741 aaaccctgag cggttaacgc ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag
    13801 aactgttcca actcggggtg attatcggca tagccacctg gctgcgtttt cgagaaggcg
    13861 ctgacgttat tgatcgccag cggaataacg cgatcgcgaa accacggtga ttcccgcgtg
    13921 gagagtgaca gttcaatctc cggtgcaagc agtcggaagg cgcagatggt ttgcactaac
    13981 tggcgttcat ccataatcga cgcaggctca atgccgccag tacacgggcg caggcgcgga
    14041 aaggagacag agtaacggct ttgccagtaa tgctgttgca gccatagcaa atgttcggca
    14101 accatatagc agtcaacgcg tcagttgtcg gaaaggccaa ttagcgcgcc gaggcctatc
    14161 ttatcaatcc ccgcacgccc cagccgatcc ggcgtttcca gccgccagaa gaagtcctgt
    14221 tttttgcctt tcagatgatg gcgggcataa gtcgcctcgt gatatgtctc ctgataaacc
    14281 atcacgccat ccagaccaag ctgctttaac tcggcgtatt ccgtcttcgc cagcggttgc
    14341 acttccattt gtagtgaaga gaactgttca cgcagggcgg ggagatgacg acgaaagtaa
    14401 tccatcccca ctttcgcctg atgttcacca gtgactaata gcagatgttc aaagcccatc
    14461 tcccgtatag cggcgctttc cctggcaata tccgcttcat ccagcgtttt gcgcttgatg
    14521 cgattactca tggaaaatcc gcagtacgtg cagtcgttag cgcaaagatt ggaaagataa
    14581 agcgggacgt agaaactaac tgtgttgcca aatcgctgac gggtcagacg ctgcgcccgt
    14641 tgggccagtt gttccagata gccactggcg gctggcgata acagcgccat catatcgtcg
    14701 cgggtgaatt gcgaggcatt tagcgcccgc tctacgtcaa cagccgtttt gccgttgata
    14761 cgcaggcgga tgtcatccca gtccagttgt cgccagcgat cgctgaaggt tttcatgccg
    14821 atgcctccag aaatccagtc agcgggctgg tggcatgagc aaaatgactg cggctgcccg
    14881 gtccggactg acgtgccagc aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt
    14941 tgacgggatc gtccgctacg gcaatcgccg tattcactaa caccgcgtcg gcccccattt
    15001 ccagggcctg cgcggcatgg ctgggaacgc cgatgccagc atcgacaacc accggaactg
    15061 tggcctgctg gataataatc tccagcatgg cacgggtttc cagtccctga ttcgagccaa
    15121 tcggcgcgcc gagcggcatc accgctgcac agccgacttc ttccagacgt ttacacaata
    15181 ccggatcggc cccgcagtaa ggcagcacga caaatccctg ttgtaccagc atttcggcgg
    15241 ctttcagagt ttcgatggga tcgggcaaca gccagcgggc gtcagggtga atctctaatt
    15301 ttaaccagtt tgtgcctaac gcttcacgtg ccagatgggc ggcgaaaatg gcttcttccg
    15361 ctgttttcgc cccggaagta tttggcagca gggtcacacc cgccgcgata agcggttcga
    15421 ggatagcgtc gttgtgctgg cgcaagtcga cacgtttcat cgccagtgtc accagctggc
    15481 tgccggaagc gcggatcgcc tccaccatca gttgtgaaga agcgaatttc cctgtgccgg
    15541 taaacagatg tgaatcaaac gttttgtccg caatacgtaa catctcaacc ccctgcaata
    15601 acctgaaaaa gcaggatctg gtcgccatcc tgcacgatat gttgcgtcca ctgctcacgc
    15661 gggacgattt gctgattaat cgccagagcc gcgcccgctt gtcgttggtc gagttgctcc
    15721 agtagttcgt gaacagtttg cccggcggcg cactgcatcg gttgatcgtt aaacaggatc
    15781 tgcattgctt cctccgcata ccgggcaacc actggcgcgg cgcaacgcca ggctgcgcca
    15841 ctggctcgat ttaccgtcga acagtcggag ttctcccgca ggtgtctcta taccgcttaa
    15901 taacttaatg gcttccagtg cctgcaaagt gcccataacc ccgaccaccg ggccaaccac
    15961 gcccgccgtg cggcagttgc gttctggctc ctggttatct ggccacaggc agcggtaaca
    16021 cccctgctcc cagggcggcg tcagtaccat caactgaccg ccaaatccga ccgcgctggc
    16081 ggtgataagc ggcgtgttga gtaccacgca ggcggcatta atctcctggc gagtcgccat
    16141 attgtcggta ccgtcgagca ccacatcggc ccgtgcaacc gcatctttta acgcctcacc
    16201 cgttaaccgt tgttgtaatg ctgtcagttg aatgtcggga ttcaactgtg tcagtcgctg
    16261 ttggctgacc tgtgatttcg ggctgttgat atcttcagtg gtaaagagaa tttgtcgtta
    16321 cagattgctt aaatgcacat cgtcgtcatc tgccagtacc agcgtcccga cgccagcgcc
    16381 cgccaggtat agcgcagcag gtgtacccag cccgccaaga ccgataatca gcacctggct
    16441 gtcgagcagt ttttgctgcc cgtcaagagc gatatcgtcg agcaggattt ggcggctata
    16501 acgcataaaa tcacggtcat tcatcgccaa ctcctgcaat ttccagcaac tgtgccgttg
    16561 ccaaacgcca gtctgcggct tgagtaatgg cgctgacgac ggcgatactg ccgacacccg
    16621 ttgctatcac cgcaggcgcg cgtgccagac tgataccgcc aatcgccacg gtgggataat
    16681 ccgccagtcg ctcaacatgc cgtgccagct gttccagccc ctgcggtgca gaaggcatct
    16741 gtttggtttg cgtcgggaac acatgtccca gcgcgatata agaggggcgt gctgccagcg
    16801 cgacgtcgat ttccatatcg tcatgtgtcg aaacacccag ccgcaggcct gccgcgcgga
    16861 tggcattgag atcggtggct tgcaaatctt cctgccccaa atggacgcca tacgcctgat
    16921 gcttgatcgc gagccgccag taatcgttga taaacaatcg cgcgttatag cggcggccca
    16981 gcgcaattgc cgccacgaca tcggcttcca cctcttcatc gcgccgatct ttgatgcgta
    17041 gctggagagt acgtacgcct gcatccaaca gacgttcgat ccactgtacg ctgtccacca
    17101 ctgggtacag tcctaaacga aaaggtacag gaggaaaatc aggctgatac atcacgcttc
    17161 ctccttacgc aggtagattt ctccgcctct ggcacggaag ttctccgaca tatccgccat
    17221 tcccatttca atagtttgcg tggcggcgta atcacgcact tcctggctga tttttatcga
    17281 gcagaatttc ggcccacaca tggagcaaaa atgggcgact ttacctgact cttgcggcag
    17341 ggtttcatcg tgataagcgc gggcggtaaa cgggtcgagg gccagattaa actggtcttc
    17401 ccagcgaaat tcgaagcggg ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg
    17461 ccctttcgcc aggtcagcgg cgtgggcggc aatcttatag gtgataagcc cctgcttaac
    17521 atcttcttta ttaggcagac ccagatgctc ttttggcgtt acgtaacaga gcatcgcgca
    17581 gccaaaccag ccaatcatcg ccgcaccaat ccccgacgtg aagtggtcat agcccggcgc
    17641 aatatcggta gttagcggcc ccagagtgta aaacggcgct tcgtggcagt gctctaactc
    17701 ctcggtcata ttgcggcgga tcatctgcat cggcacgtgg cctgggcctt caatcatcac
    17761 ctgcacgtca tattcccagg caattttggt cagttcgccc agcgtatgca gctcggcaaa
    17821 ctgcgcttca tcgttggcgt cctgaataga accggggcgc agaccgtcgc ccagcgacag
    17881 cgaaacgtca taagcggcac agatttcaca aatttcgcgg aagtgttgat agaggaaatt
    17941 ttcctgatga tgggagaggc accatttcgc cataatcgaa ccgccgcgag agacgatacc
    18001 ggtcaggcgt ttcgcggtca tcggcacata gcgcaacagt acgcccgcat ggatagtgaa
    18061 gtaatccaca ccttgctcgg cctgttccag cagcgtgtcg cggaacgctt cccaggtaag
    18121 atcttcggcg atcccgttaa ccttctccag cgcctggtag atcggcactg taccgatcgg
    18181 caccggactg ttacgcaaaa tccactcgcg ggtttcgtga atatagcgac cggtggagag
    18241 atccatcacc gtatccgctc cccagcgcgt ggaccatacc agcttttcca cttcttcttc
    18301 gatggaagag gtgaccgccg agttgccgat attggcgtta acttttacca ggaagttacg
    18361 gccaataatc atcggctccg attccggatg attaatgttg gccgggataa tcgcacgtcc
    18421 ggcagcaact tcatcacgga caaattccgc agtgatattt tccggcagac gtgcgccaaa
    18481 gctcattccc ggatgctggt ggcgtaaaac ttcgctacgt atgcgctcgc ggcccatatt
    18541 ctcgcggatg gcgatgaatt ccatttccgg tgtgatgatg ccctggcggg cgtagtgcag
    18601 ttgggtgaca cggcgtcctg ctttggcgcg ttttggtgtt agtacgccgc taaaacgcag
    18661 ttcgtcgagg ccatcatctg ccagccgcgc tttagtgtaa tcggaactgc gcacggtaag
    18721 ttcttcggta tcgccgcgcg catcgatcca cggctggcgt agttttgcca gcccttgctg
    18781 cacgttaatg gcgatctgcg gatcgccata tgggccggag gtgtcgtaga ccggaatcgc
    18841 ttcgttttct tcaaactgca gctgttcttt gctgccgccg attagcgtcg ggctaagctg
    18901 gatctcacgc atcggcacgc gaacgccaga gtgtgtgcca gtgaggtaaa tgcgttttga
    18961 gttgggaaag gcggtgcctt ccagggtgtc gataaaatgt tgggcccggg cgcgttgttc
    19021 gcggcgggtc agttttgttg cagacatagc tcattccaaa aagttaagga cgtggcttgt
    19081 cagacgacgg atgaagcaag agacgatcgc cgcaaaggcg atgcgatagc agattaactc
    19141 ttgttccctt cgcaggtatt agcctgatca ggttccgcgg atcccgaata aacggtctca
    19201 gccagttaag gcactccgac aagaaattag cccccacaaa cggggcgttg aatgtaaatt
    19261 acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc
    19321 agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat gtcagataaa
    19381 acctgttgaa actcaaggta attatcatga tcgatggcgg tttccaggct ggaatcgtag
    19441 taatccataa tctgttgggt attggcttcg agttgcggcc aaatcttcgc ggcgcgcgcg
    19501 agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa atgtccggca
    19561 gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg
    19621 tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag cagatgctta
    19681 cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta
    19741 tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc tcagtgagaa
    19801 atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc tatggatcgt
    19861 ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa attatggttg
    19921 ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca
    19981 ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca gcggcgtcac
    20041 gatatggggt cggtacgtca ggtcattgat ctcgatgttg tgctgtttca actggccgga
    20101 cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta ctgcgggcat
    20161 gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg tgagcgttac
    20221 tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc gatcctcctc
    20281 gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt cgtcgaagtg
    20341 ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg aattaaagtt
    20401 aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg
    20461 tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg
    20521 aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt
    20581 ctgatagaag atactgtggc gatgtgtcgg gcagagtatg agtgatgata cactgaccgc
    20641 ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg
    20701 ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc gggtcgctat
    20761 ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac
    20821 gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg
    20881 atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct ctattttgaa
    20941 gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt cgataagctg
    21001 ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg taccattcgt
    21061 cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg gacgctggca
    21121 acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat
    21181 gccgatccgc aggcgctgca cgctctactc gataaactgg cgaaaagcgt cactttgtat
    21241 ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac ctggggcggt
    21301 gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa aattgttgat
    21361 ggtttactgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac caaaggcggc
    21421 ggacagtggc tggaagcgat ggcagaaacc ggttgcgatg cgttgggcct cgactggaca
    21481 acggatatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca gggtaatatg
    21541 gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc gactatactt
    21601 gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat tcatcaggat
    21661 gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc tgaacaatat
    21721 caccgctaag gagtgattat ggatctcgcg tcattacgcg ctcaacaaat cgaactggct
    21781 tcttctgtga tccgcgagga tcgactcgat aaagatccac cggatctgat cgccggagcc
    21841 gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct gctgaaatat
    21901 ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat gccttacatt
    21961 ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg
    22021 caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg ccgtcttggc
    22081 gtcgccagcc attttggttt gatggtggat gtgccaacga tcggcgtggc gaaaaaacgg
    22141 ctctgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc actgatggat
    22201 aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc
    22261 gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa
    22321 ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg
    22381 ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa tttccgtatt
    22441 tgagaactta acatgttaca aaacccaatt catctgcgtc tggagcgcct ggaaagctgg
    22501 cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta cgccatgttc
    22561 tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gtattctcga tctcatctgg
    22621 gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga gaaatttgaa
    22681 gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc aatcgatgcc
    22741 tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg
    22801 gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac tcaggttggt
    22861 cgcgaaatga gcgatgaaga gcttaaagaa aacccagctg tagagcaaga atgggacatt
    22921 cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga gctgataaaa
    22981 ggccttaggg cagacctgcg tgaggcgggt gagagcaata ttggtataat ttttcagcaa
    23041 taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt ccctttcgcc
    23101 ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga
    23161 gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa cacattgtaa
    23221 ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc agaactgtcc
    23281 aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga gtctctgaaa
    23341 gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca ccgcgctgag
    23401 cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg
    23461 gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt ggcagtgaac
    23521 agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc attgatgctg
    23581 gctggcgctc tgcttctcac tgcctgtagt cataactctt cacttcctcc ctttaccgcc
    23641 agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag cggcgataat
    23701 gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac gcgagagtat
    23761 cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa accgccggtg
    23821 aatattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg tgaatccgat
    23881 ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg tgctgatcag
    23941 atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg
    24001 catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc tgatttagat
    24061 tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga tgtcagcgtg
    24121 gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc tgatttctgt
    24181 cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc atatgaccgc
    24241 caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata cccgcttcag
    24301 ccatcgcgat atctcgtttc acccgtaact catctaacga ctgacgcaaa ttctccatct
    24361 ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac agggcattgt
    24421 attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagcg taaaagtcat
    24481 tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc
    24541 acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg
    24601 ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca gtggttgtct
    24661 tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg
    24721 catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt ttactcattg
    24781 aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg catgatttct
    24841 gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga
    24901 ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc tggtagggtt
    24961 gtttgcggtg acggtgattc gcgattatgg gcgcgagact gccgccgcca gacaaacgct
    25021 gctggaaaaa ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg
    25081 gatgcgcatg catcatgcgc agcagcaggc cctactggaa gaaatggccg ggcagcctgg
    25141 agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata gcaactccgg
    25201 catggtggga aaacagcttt attccccgca ggaaatgcag cagttacatc cgggagatga
    25261 agaagcgtgg cggcggatcg atagcgcaga cggtgagcct gttctggaaa tttatcgcca
    25321 gtttcaaccg atgtttgctg ctggaatgca ccggatgcgc catatgcagc agtatgccgc
    25381 gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg ccgaagatcg
    25441 tgagcagaga aacaccctga ttatcctctt cgccctggcg acggtcttgc tggcaagcgt
    25501 attgtcattc ttctggtatc gccgctatct gcgctcgcgc cagcttctac aagatgaaat
    25561 gaagcgcaaa gagaagctgg tggcactggg gcatctggcg gcaggcgttg cccacgaaat
    25621 ccgtaatcca ctttcctcaa ttaaagggct ggcgaaatac tttgccgaac gcgcgccagc
    25681 agggggagaa gcgcatcaac tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg
    25741 cgtggtaagc gagttgctgg aactggttaa gccaacgcat ctggctttgc aggcggtgga
    25801 tctcaacacg ctgattaacc actcattaca gctggtaagc caggatgcaa acagccggga
    25861 gatccagtta cgctttaccg ccaacgacac attaccggaa attcaggccg atccggacag
    25921 gctgactcag gtcctgttga atctctatct caatgctatt caggcgattg ttcagcatgg
    25981 cgtgattagc gtgacggtca gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag
    26041 cggtaaggga attgcggcag atcagcttga agccatcttc actccgtact tcaccaccaa
    26101 agccgaaggc accggattgg ggctggcggt cgtgcataat attgttgaac aacacggtgg
    26161 tacaattcag gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct ggcttccggt
    26221 caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat tctggtggtg
    26281 gatgatgaca ttagccactg cactattttg caggctttac tgcgcggctg gggctataac
    26341 gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat
    26401 cttgtgcttt gcgatgtgcg aatggcggag atggacggca tcgccacgct gaaagagatc
    26461 aaagcgttaa acccggcaat tccggtgctg attatgactg cgtactccag cgtcgagacg
    26521 gcggtagagg cactgaaaac tggggcgctg gattatctca tcaagccgct ggatttcgat
    26581 aacctacagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga tgctgaaaca
    26641 cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggtgat gcaacacctg
    26701 ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacag tgctgatcca cggcgattcc
    26761 ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa
    26821 ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc tgaattgttc
    26881 ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg ctttgttgag
    26941 gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat gatgcaggtg
    27001 cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc
    27061 tcggttgatg tccggctgat tgcggcgccc catcgcgatc ttgccgcaga ggtgaatgcc
    27121 gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga agtaccatcg
    27181 ctgcggcaac ggcgggaaga tatccctctg ctggctggcc attttctgca gcgctttgcc
    27241 gagcgtaatc gcaaggcggt aaaaggtttt acgccccagc gatggatctg ttgattcatt
    27301 acgactggcc gggaaatatt cgtgagctgg aaaacgcggt ggaacgggca gtggtgctgc
    27361 tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt acgccgatcc
    27421 cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag gtgattctgg
    27481 cggcactgga gaaaacgggc ggcaacaaaa ctgaagccgc ccgtcagtta gggatcacgc
    27541 gtaaaacgtt attggcaaaa ctgtcgcgtt agttctgctc gcgttcgata gcacgccagc
    27601 cgatatcttt ccggcagaag cagtcgtccc agtggatatc ggtcattaag gcataggcgc
    27661 gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg cgcccgccgt
    27721 tggtcactac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact ttgccgcctg
    27781 ccacttcttc cagcggcagg ccgtggatca cgtcaccggt gcggtaatca cccggatatc
    27841 cacccgcagc catcaccacg ccgagagaag cgcgttcatc ccactcggac gttttctcgt
    27901 ccagtttgct ttcacaggcc gccaggcaga gctcaaccag atcggacttc atgcgcagca
    27961 taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc
    28021 cctgtttgtc gatcatcagg cccgcgtaga gaaaaccggt gtaggtgttg ccttccgccg
    28081 ccatgccttt cacggttggc cagataatac gttccatggt gcgctgatga acgtcatcgg
    28141 ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg gtatctttat
    28201 cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg ccgtccacca
    28261 tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg cgatgacccg
    28321 cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct tcttccagcg
    28381 tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg acgattggcg
    28441 cgcctttctc acgcagatac gccagcgcag gttctacctc ggtgaagttc tggtattccg
    28501 ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag ccttccagtt
    28561 gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag gtatcgacca
    28621 cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt tcgttttgtg
    28681 cgaaatccaa cagcgccggg atatcggtca cgccaatagc aacgttttgc agcgcgggtt
    28741 ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc ggcgactggg
    28801 ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat actttcatct
    28861 attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc
    28921 gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt
    28981 cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg cgtcagaagc
    29041 catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg cgattttcgc
    29101 ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt ttttggcata
    29161 gacgatagca ttggatttca cgaacttcgc cactttccag cagaacagcg catcgcgcag
    29221 ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac cgaccatccc
    29281 caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac
    29341 acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt
    29401 cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac gagaaatgat
    29461 ggcctgtgcg gtttccgcat ctagctcgcg gttaaaggca atgatgccgc cgaatgcgga
    29521 ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc cgatagctac
    29581 accgcaaggg ttggcgtgct tcacaatcac acatgctggc tcggcgaact ctttcacgca
    29641 ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac cctgaacctg
    29701 ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg cagcctgctg
    29761 gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg
    29821 tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca tgctgccgaa
    29881 gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc
    29941 gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa taatggcgtc
    30001 gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg cggagcgcac
    30061 catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc agccttcacg
    30121 agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag gctggatctg
    30181 atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac catgtacttt
    30241 cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt aatcggaaac
    30301 ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg tagacagcag
    30361 ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt tgtcagaaac
    30421 actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc tggatttgac
    30481 tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc cctaacgggg
    30541 catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg cgaatttttc
    30601 tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac agaaattaca
    30661 ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat
    30721 gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa ctccctataa
    30781 tgcacctcca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    30841 gagaaaaatc ctgaaattta gggttgactc tgaaagagga aagcgtaata tacgccacac
    30901 tcgcgacagt gcgctaaagc gcgtcgcaac
//
LOCUS       DAJTMK010000030        30688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTMK010000030 DAJTMK010000000
VERSION     DAJTMK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30688
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.031"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(148..4608)
                     /gene="mukB"
                     /locus_tag="OO135_001170"
     CDS             complement(148..4608)
                     /gene="mukB"
                     /locus_tag="OO135_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5397095.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFSLTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4608..5312)
                     /gene="mukE"
                     /locus_tag="OO135_001171"
     CDS             complement(4608..5312)
                     /gene="mukE"
                     /locus_tag="OO135_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5397096.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5293..6615)
                     /gene="mukF"
                     /locus_tag="OO135_001172"
     CDS             complement(5293..6615)
                     /gene="mukF"
                     /locus_tag="OO135_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5397097.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6612..7397)
                     /gene="cmoM"
                     /locus_tag="OO135_001173"
     CDS             complement(6612..7397)
                     /gene="cmoM"
                     /locus_tag="OO135_001173"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5397098.1"
                     /translation="MQDRNFDDIAEKLSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7533..8312
                     /gene="elyC"
                     /locus_tag="OO135_001174"
     CDS             7533..8312
                     /gene="elyC"
                     /locus_tag="OO135_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5397099.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8289..9182)
                     /locus_tag="OO135_001175"
     CDS             complement(8289..9182)
                     /locus_tag="OO135_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5397100.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9336..10082)
                     /gene="kdsB"
                     /locus_tag="OO135_001176"
     CDS             complement(9336..10082)
                     /gene="kdsB"
                     /locus_tag="OO135_001176"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5397101.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10079..10261)
                     /gene="ycaR"
                     /locus_tag="OO135_001177"
     CDS             complement(10079..10261)
                     /gene="ycaR"
                     /locus_tag="OO135_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5397102.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10313..11545)
                     /locus_tag="OO135_001178"
     CDS             complement(10313..11545)
                     /locus_tag="OO135_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5397103.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQVITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11582..12568)
                     /gene="lpxK"
                     /locus_tag="OO135_001179"
     CDS             complement(11582..12568)
                     /gene="lpxK"
                     /locus_tag="OO135_001179"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5397104.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12565..14313)
                     /gene="msbA"
                     /locus_tag="OO135_001180"
     CDS             complement(12565..14313)
                     /gene="msbA"
                     /locus_tag="OO135_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5397105.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14350..16614)
                     /locus_tag="OO135_001181"
     CDS             complement(14350..16614)
                     /locus_tag="OO135_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR5397106.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     IGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPNEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VYDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16821..17105)
                     /gene="ihfB"
                     /locus_tag="OO135_001182"
     CDS             complement(16821..17105)
                     /gene="ihfB"
                     /locus_tag="OO135_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5397107.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17265..18938)
                     /gene="rpsA"
                     /locus_tag="OO135_001183"
     CDS             complement(17265..18938)
                     /gene="rpsA"
                     /locus_tag="OO135_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5397108.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(19049..19732)
                     /gene="cmk"
                     /locus_tag="OO135_001184"
     CDS             complement(19049..19732)
                     /gene="cmk"
                     /locus_tag="OO135_001184"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5397109.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19905..20669)
                     /gene="ycaL"
                     /locus_tag="OO135_001185"
     CDS             complement(19905..20669)
                     /gene="ycaL"
                     /locus_tag="OO135_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5397110.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMNMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAVNAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(20838..22121)
                     /gene="aroA"
                     /locus_tag="OO135_001186"
     CDS             complement(20838..22121)
                     /gene="aroA"
                     /locus_tag="OO135_001186"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5397111.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(22192..23280)
                     /gene="serC"
                     /locus_tag="OO135_001187"
     CDS             complement(22192..23280)
                     /gene="serC"
                     /locus_tag="OO135_001187"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5397112.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(23479..24171)
                     /locus_tag="OO135_001188"
     CDS             complement(23479..24171)
                     /locus_tag="OO135_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR5397113.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            24301..26061
                     /locus_tag="OO135_001189"
     CDS             24301..26061
                     /locus_tag="OO135_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5397114.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            26467..27324
                     /gene="focA"
                     /locus_tag="OO135_001190"
     CDS             26467..27324
                     /gene="focA"
                     /locus_tag="OO135_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5397115.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            27379..29661
                     /gene="pflB"
                     /locus_tag="OO135_001191"
     CDS             27379..29661
                     /gene="pflB"
                     /locus_tag="OO135_001191"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5397116.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            29853..30593
                     /gene="pflA"
                     /locus_tag="OO135_001192"
     CDS             29853..30593
                     /gene="pflA"
                     /locus_tag="OO135_001192"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5397117.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         7537 a         8235 c         7418 g         7498 t
ORIGIN      
        1 aggagaggcc ctggaaatac cccacagccc cgaaaaagag ccagtcagtt gcttaactga
       61 ctggcattac gccgttatgt gccatttttt tagtgcagaa acggagtttt cggaaaaaga
      121 aaaggcggca ttgctgccgc cttaatttta actcgcctga gaaggcgctt cgtcagttcc
      181 tggaagcgtt tcagggagtt gcggcgcaaa tcctcgcagg ccgacgacat gaacatgttc
      241 ggtattctgg aagactttac gcaccagttt ataggtggtg cctttctccg ggctgatatt
      301 ttccggcgct gcgatgatga gctgcatttg caaacgctca cacaattcaa acagcgtggc
      361 gatagaacga gcatccagtc gcgctgcttc atcgaggaac agcagacggc aaggagagat
      421 atctttaccg cgcaggcggc gagattcatc ttcccagctt tgtaccacca tcaccagaat
      481 cgacataccg gtaccaatcg cctcaccggt cgacaatgca ccagactctg cgcgcaacca
      541 accatcggaa ccacggttaa cctcaacttc catttccaga tagttgcggt aatccagcag
      601 ttcttcgcca atggtctgcg gcgtgcgctg ccccatatca atctgcggat taagacgctg
      661 atacaatttc gccaacgctt ccgagaaggt cagacggttg ctgttaaaca gatcctgatg
      721 ctgctcgtgc tgttcagaga gcacatccag cagcatggcg tgcgtttcac gcacgttcac
      781 gttgaggcgc acgctgttca cctgaccaaa cgatacgttc tgcaacccct ggttgagcat
      841 acggatacgg ttctgctcgc gctgaatggt tttgcgaatg atgttcgcca cgctgcggga
      901 actgatcacc agtttctgtt cacgggaggt taattcttcg gtcagacggc taagttcgat
      961 ctccatctgt tcgatagctt ccaccggatc atcggtacga ataatatcct gacgaatacg
     1021 ttcacgcaga tgctgataag ccgccacgaa gaactgaatt ttacgctccg gacgtttcgg
     1081 atcttccgac atgcgcagca cgtcgcgcag atgttcgtta tccgccaccg ccagacgcag
     1141 cgcacccaat gccttatccg acatagagcg caaatcatca gcggagagat aagccagctc
     1201 acgacggtgt aagcggcgct caacgccgtt atctttcacc atgcgcatca ccgcacacca
     1261 gcccgctttg gcggtcacta cctgctcgcg catctcaaag taatcccgct ccagcttgcg
     1321 cagtttgcgg gtcaggttgt ccatctccgc ttcgcagaag gtaagcgctt tttccagttg
     1381 attgcggcgt gaacggttat tgctcagttg cgcatgcagc tcgtcacggc gaatacgcgc
     1441 ccgctcttct gccccgctat cagcacgcac gccgatatcc tgcaattcac gttgcagatc
     1501 gttgagtagc tcttttttgg tgtcgtaaga acttttcagc gaagccagca cctggttgta
     1561 ctgactcaac tgcgctgcgt gaccgcgcaa cgcttcgcga gcgcgggtac gctccgcttc
     1621 cgcctgttcc agacgttcac gcagtttttc gttgagatcg ctgttaccgc taagcatttc
     1681 tgccgagtca gagtagctaa agtgcgcacg acgctgcacc acttccgtca gggaaaacgc
     1741 ctgctgacgg gcatcgcgct gcatctgttg agagtacgcg taatcttctt ttaactgttc
     1801 gaactgttcc gggtcgctct gcaataccga aacgatcggt tccagtttcg ccagttgatt
     1861 gccaaactgc tgaacaaaac gcgcggcttc ctgggcttca tccagacgtt cgcggatttc
     1921 atcgacgcga tccgccaggc tatcatcagc caacaggttg agacgcggca gaatgcggtt
     1981 cagcgccgta acgccctctt tcgcctgctc aaactgaata cgctgctgct ggttatcatt
     2041 ttcatgatta cttaacgccc gctccagttc gacgcgacgg ctgttcagtt gacggatttc
     2101 tgcttccggg tcagactcaa acacaaccgc cagatgactg ccgataaagc ggctgaacgc
     2161 ctgatgcaga cgctgagttt tctgtacatc aaaggagagc gtggcgaagc gttcggaaag
     2221 cacttcacgc tcggcatgga ggctttcaat acggctttca cgcgcagcac gaccaaacag
     2281 cggcacttcc gggaaacgtg aataacgcca ctgacgatcg gcgattttca ccactaccgc
     2341 tttttccagc tcatcaacgc tgaacacgct gtcatcgaat gactgcggat ctccttcgat
     2401 cagatagaga tcttccgggc aatcggtcaa gccatccagg tgttcagtta cctgtgacag
     2461 atctggcacc acgatggcgt ggcgtgacgg gccatacagc gctgagaagt acggcgcatc
     2521 ttccaggcta acgtcgtcat aaatttctga cagcagcaca ccaccaaaac gctccgccag
     2581 cgcgttcaga cgctgatctt cagagccgcc aggctggctt aaacgttcga tctcttcatc
     2641 gacggcgttt ttgcgcgcgc ccacttcatc gcgttcaaca atcgcctctc gctcacgctc
     2701 cagcaactgt tgcagatatt cggtgacgtc ctggctggag gtaaactctt cgccgcactg
     2761 ttcgctcaac tggttgagac tgttttgcgc tgccagccaa accggcgcac gctgcatcaa
     2821 actctgaatg cgagactgca gctgttcctg ctcctggcgc agtgccatgc gctcttcacg
     2881 ggcgttagac acgctatcgg aaagagaggc aatgcgtgct tccagttcct gatgcagggc
     2941 ttccagttcg tcgatatcaa aattcttgcc ctgacgtttg cagaaatctg ccagcagacg
     3001 ctcagcttct tgctgctcgc gtagacgctg ttccagttcg cttaatcgca tccgcagcgg
     3061 ctgaacctgc tctgccaggt gacgctgatc gaccccttcg cgcaatagtt cgcgagcgac
     3121 atcccacgcc tcgttacgcg ccagtgggcc gttgattgcc accaccagct gataagcctg
     3181 ctcaaactgg ctgtgcgcgg tttgcgccat gctcattttc tgctcaagag agagcatttt
     3241 ttcagtcgct tccagctctt tcgcctggaa ggtttccagc cattcggcgg cgctgtcggc
     3301 ggttaagtcc ggcagatggc acagttcttt ggcacgatta agcgcagcaa tcgcctggtt
     3361 atactggatc gcgcgcgttt gctggacgtc cagcgcctgc tggtagtcag caagctggct
     3421 tttcagctcg tccacttcca gttcggcagc ttccgcgcga gcctcgttct cttcctggcg
     3481 ttcgatggct tctgccacca cttcgttttg ctcttccaga cggatctgca gctcatcgag
     3541 atccgcttcg tagcgttcga tcttctcctg ctgacgcagt gcggtttgca ccaggttcag
     3601 gtgatcgctg gccgcctgat aatccgcttc cagatcgcct tcggcaccgt tgtgctctgc
     3661 cagctcacgc gccatatcga cgtgtttgta ctgctcagcc gccagttgct gacgcgaagt
     3721 atgtaactcg cgacgaaact ccagggcttt gtcgagatgg acacggcgct cgttggcgtg
     3781 acgcatgtag tccgccgcca cgtagttggt agcttcgctg atcagatgct taaacaggtc
     3841 acggtccgac tgggtgacac gaatcgcttc cagcgtcatg cggttttcac gcagcgccgc
     3901 ttccatgtcc tggaacgctt tacgcacgcc gctgttttcc ggcaacaggt agtcgcgcag
     3961 agaacgggta atggcactgg agatcccgcc atacagcgaa gcttcgatca gacgatagaa
     4021 tttgctacgg tcagatgcgg agcgcagacg acgcgcgatg atgcccagat cgaacatcag
     4081 cgagtggtaa tcggtaatgg agttgaactg tttaaactgc acgccttcca tcgcctcgag
     4141 cttgtctttc agctcgttaa gcggcagcac gcgcgcctgg cgttcgttca gggtttcggt
     4201 caccagctgt gtcggctgca ccgacatcgg cagtccctga atggcaaatg gtttgatatc
     4261 cactttacga tcgcgtccgg caacctgttg cagacgcaca ccgaccacca cgcgctggtg
     4321 gcgcgagtta atggtgtcga gcatcgaata acagacaccc gctttcagct taccgtgcag
     4381 acctttatcg cgcgaaccgc tggtggcccc ggcttccgta gtgttacgga aatgcagcag
     4441 ggtcaggtcg gggatcagcg ccgtaacgaa cgccgccatg gtggtggatt tacccgcccc
     4501 gttaccgcca gaaagcgtcg tgaccagctc gtcaaggtca aaagttcggg caaaaaagcc
     4561 gttccagtta atcagcgtca gtgagcgaaa tttaccgcgt tcaatcatta ttcttcctct
     4621 ccgctatctg gctgattctc ttcggtttca tcgttgagtt gcagatgatt ttcaatcggc
     4681 attgcttcgc catcacgaat caggcgacgc tgtgcttcac ggggatcgtc gccagcacgc
     4741 acatcggctc cgaagcggaa caccgattcg gtaatgcgaa acttgctgct gtcgtggccc
     4801 ataaaccaca ccatgcctaa acgacgcaga cggttgagcg aagaacgtac tttctcctgc
     4861 aacttctgac ggtcaacgtc tgaaccggtt gaacggttgt tcaccagttt cagcagtttt
     4921 gcttcatcgg ccagggtgag cagttcgtcg tacagttcct gctgggtgaa aatcccctca
     4981 ttcgccagcc gttccgggct gagatagaga taacagagga ttttcccgac catcatatcc
     5041 agttccgaca agacggaacg cgggataaga gtggtggaac gtgggcgtaa atagaagaac
     5101 ccttccggtg cgcgaataag ctcaacgtta taacgcgcgt aaaactcttc cagatattcc
     5161 tgaaaatcca tcaggaatgc atgattatcc agttcgtcga ggccaatatg gcggcctgaa
     5221 cgtaaggcgc tgtccagcgc cggaaataac ggattcgcca gcgcctgcgc cagcttaacc
     5281 ggcatcactt gttcaatatt tgtcgatgac atgcgcctgt accttggctc cgtaatcatt
     5341 aatcggctgc catttcgctg gcagtccggt gaaatctgct tgcgctacgc caagacgtac
     5401 cgcctgatca ataacaatac gcgcaacgtc aaagtgacgt gcacgcggat actgtgacag
     5461 atattcgcgt accaccagac caagatccag cggcacttgt ctggttttgt acacggcaag
     5521 ttgttcttcg atgatcgccg ccagctgttc gcggatctcg ttaaactctt cgtattccag
     5581 atcctcagga agttccccag tcacttcttc atcgcgcagt gccatctctt cgtcacgcat
     5641 atccagcaga cgatcggcat tggcataagt tagcgcccac ggctcatcaa aataggtttg
     5701 taccgactga cgtaaccgct gagcaaagac gcggttttta tccatatcga tcgcggtacg
     5761 aataaatttg tgtacgtggc ggtcgtagcc aatccacaag tcgatggatt gctggcccca
     5821 actgataata cgatcgagtt tgctctgcag atcgaacacc agacgatcga cgaaatgcag
     5881 atcgtcatgg gtcatcgtcg catcctgaat gcgcaacaga ttagcctgca atttgtcgcc
     5941 tgccgcttcc agcgtatcct gcaattcacg cagcgttccg gaagtttccg aaagcaacaa
     6001 ttcacagctg gaaattgccg cccgccagtc tttgttcagc aactgggcga tatcgtcctt
     6061 cacctgctgc tgctgttcgt ccatcagacg ttgcgtcagg tcgatactgt cgaaaatttc
     6121 tgctaccgag tatttcagtg gcgcatagac attacggtgc cagtgaaatt catcaccgcc
     6181 ctcttcggcg gcatctgctg cgcgtttgag ctcacccgcc acaatcgaca actgcataga
     6241 aagacgcagc gtagaaaact cgcgctgacg gatgtagtag tcagtaatgc cgatgccgag
     6301 cggcgtcaga cggtaaattg cgttcccttc cgcctgctcg ctggtaaagc ggttcagcag
     6361 acgttgacgc accatgtcgt tgatcgcgtt attggcgcgc acgccgatgg tttcgctggt
     6421 ttgctcaaac gcatcactca catggcggaa tgcatccacc agctcgcctt cactcatctc
     6481 accatccaga cgctcgccgt tcagcgtggc aaccgccagc agaaaagaga gtcggtctac
     6541 cggcagcgag atggagaagt cattttttct ggcccaggca accagttcgg ggactgtctg
     6601 ggaaaattca ctcatacttt atccttgctc tgcggtttgc gcgcggtgac atgaatataa
     6661 cgccccaggg taatatacgg ttcctgacgg caataacgcg tttctaattc aagtaatgct
     6721 tcatagcagt cgcgctgctg gtgcttctcg cgcagataat catgaaacac gcgaacgcct
     6781 gtcttaccca taatttgcca accagcttct tccagccaca gataaacctg cgccgggtcg
     6841 cgtggataat ctggcgaaag cgtccgtttt ttctttttcg gcattcccgc ctgcacgtaa
     6901 tcaaaattcc cggcgaccat gttatgcatc aacaaaccat gcgcattgta gaacattaac
     6961 gacaacacgc cgcctggacg caacactgac cacagggtct gcaatacact gcggggatcg
     7021 gccacccact cgagcaccgc atggaacaat atcagatcaa cgggcgtttc caaatgcgaa
     7081 gcaacatcct gagcggcgca atgtataaat tgcatgttgt cgctcacacc ttttgcttct
     7141 gccgcctgtt ttgcgcggtc gatcatctgc gcagaaagat cgcataaaat gacctgatgc
     7201 ccacgctcgg ccattttgat tgcggtctgc ccttctccac cgccagcatc cagcacacgc
     7261 agtttttgcg ggcccatttc cgccagcacg cgatcgagat cctgccacag aatagcctgt
     7321 cgaagctgcc ctttggtggt gccgtaaatg ttacgggaca acttttccgc aatatcatca
     7381 aaattgcgat cctgcattgc tttctccacc ggttggctaa gacgttttct gataaagacg
     7441 ctattttgtc acagccacgg cgagaatgaa cccgtctggt gtaatatcgc tggcgatccc
     7501 ttgctatatg gttaaaaaag gaaccagaaa ggatgctttt tacactgaaa aaagtgattg
     7561 gcaatatgtt gctgccactt ccgctgatgt tgttaattat tggcgctggc ctggcgctgc
     7621 tgtggtttag ccgttttcaa aaaaccgcga agatttttat tagcattggg tggctggcac
     7681 tcttgttatt gagtttgcaa ccggtagccg accgcctgct gcgtcccatc gaaagcacct
     7741 atccgacatg gaataattcg caaaaagtgg actatatcgt ggtgctcgga ggtggttata
     7801 cctggaaccc gcagtgggca ccgagctcta atttaatcaa taacagtctg ccacgcctga
     7861 atgaaggtat tcgcttatgg cgcgaaaatc cgggatcaaa actgatcttc acgggaggcg
     7921 tagcaaaaac caatacggtg agtacagcgg aagtaggtgc cagagttgcg caatcgctgg
     7981 gcgtgccgcg cgagcaaatt atcaccctgg atttgccaaa agataccgaa gaagaagctg
     8041 cggcagtgaa acaggcgatt ggtgacgccc ctttcctgct ggtgacctcc gcatcacatc
     8101 tgccgcgggc gatgatcttt tttcagcagg aagggttaaa tccgctacca gccccggcga
     8161 atcagttggc catcgactcg ccgcttaatc cgtgggaacg ggcaatccct tctccggtct
     8221 ggctgatgca tagcgatcgt gttggctatg aaacgcttgg ccgcatctgg cagtggctga
     8281 aaggtccgtc aggcgagcca cggcaagagt gattttgatg ccagatcgaa gttgcgccga
     8341 ctaaatcgtc cggtattaac cagttgcgca acttcatccc ataacacata caaccaacgc
     8401 cgccagatga acgactccgc cactggcgcg cgttgcaggt aactccagag caaatcttcc
     8461 gccagagaat tatccattag tcggaacagt tcgtattctc gcggtgccca aagcattagt
     8521 cccggcccga ccatcgccag taactgatcg ctgcgcgaat ctttcagcat gctgcgtaaa
     8581 cagaagttac cgtgaatcaa cacacaattg tcgttaaagc cttcaaataa tgccgggaga
     8641 cattcgcgag tgcgaaacag gatccgctta tcctgcatcg tcagaccggt gttattgaac
     8701 tgattgagcg tggtccatag cacttcaaca tgttgccggt accatgaggg ccagaaattt
     8761 tcctgagtat tgtcgaccgc accgacgcaa ccgcgactgt cctgacggtg ccaggccagt
     8821 aaggcttcaa cgatttggtc tttgagctgt tcccagcgtt ctggtgttcg ggctggtgcc
     8881 tccaccgaaa caccacgcat ccgctccagt agcaggacat cagggccggg gtgttcttca
     8941 tgcgtcatca caccataaat agtcggcata cggacagtcc cacttcttgc cagcatggtg
     9001 gttttccatg ccagttgtcg ggcttttccg ggcgtactaa agcttcttgc cattaacggc
     9061 attgggtttc cctggctgtc atacaaagcc cacaacgccg tatccgcttt ttcattgacg
     9121 cactcaatac gactgagttt ttcgcccagt aaatggctta attcggcacg cagctgttcc
     9181 attcagatta ccctcataaa agctactgct ttcataatga gcgccaatat aaaagatgtc
     9241 acctgaatga gatcaattgt tgaaaagaaa aattgaggaa aatcgtaacg gtacgacact
     9301 cctcccaaaa ttggctgaag tgtcgtgaag tgaaattagc gcatttcagc gcgaacgcgc
     9361 tcaagatctt caggggtatc cacacctgtg ccaggaactt cctgagcaac agcaacatgg
     9421 attttttcgc cgtaccacag aacacgaagc tgctctaaca tttcgatgtg ttctaacgga
     9481 cttgcctgcc agttgacgta acgacggata aagcctgcac ggtagccata aatgccaaga
     9541 tgacgcagga agttatcgcc aacggtttca aggccttctg caaaacgatc acgatcccaa
     9601 ggaatggtgg cgcgtgagaa gtacagtgca tacccttcag cgtcgagaac cactttcacc
     9661 gcattcgggt taaacgcttc ttccgcattg tggattggca ccgccagagt cgccataccc
     9721 acctgacgct gagcgaggtt atcagcaacc tgacgaatga ttgtcgcagg gatcatcggt
     9781 tcatcaccct gcacattaac gatcaccgtg tcgtcgctga atgcgcattt ttcgacaact
     9841 tccgccagac gttctgttcc tgactgatga tcggcgcgcg tcatacatac ttcaccgcca
     9901 gcggcttcaa cggcgcgggc aacatcctca tgatcggttg ccacgatgat gcgctcggca
     9961 cctgattcac gcgcgcgttc aagaacatga acaatcatgg gtttgccgtt aatatcaacc
    10021 aatggtttac cgggcagacg cgtggacgcg tagcgcgcgg gaataatgac cacaaaactc
    10081 atgatttact ctcatcagca gtcagcacgc gggcttccgt ttccagcaac actggaatgc
    10141 catcgcggag cgggaaagcc aggttatcca gtttgcaaat aagctcttgt ttttcctggt
    10201 tataccaaag ttttccgttg caaaccgggc aggcaatgat ttcaagcaaa cgatgatcca
    10261 tagttcctcc agatggatcg ggttatgaat gcataaatct tatcataatc atttatgcga
    10321 cggggtctat ttcccagcca gtgcggcaat cagtaaagag accttgcggg cagcgtccta
    10381 atgtcacccg cgttgcctgc tgccagctcg cgaaatcagt aatcacctga cgtaaccctt
    10441 tttgcagcat tgtcgttggt ttaatgcctt cctgtaacca cagagagata acttcaagga
    10501 tgcctgtctg gcgatgcatt ttggcatcca ttcgcccaac taattgccca cgatgtaata
    10561 atggcagaac aaaatagcca tactggcgtt tcggcgctgg ggtatagcac tccagccggt
    10621 agctaaaatc aaaaagctgc tctgcgcgtt tgcgatccca gacaacagga tcaaaaggcg
    10681 aaagtaccgc gctgtgcgtt gcagtgagct ttccggccag cgcacgctcg agtagcggca
    10741 gcaaatcagc atgcagccaa agattgccca atttttcaac atgcacagca atgatttgct
    10801 gctgttcagc cctcgcttcg cgccacgccg ccagtgccgg acgtttcagc cgatagtaat
    10861 ctgccagcca ctgttcgcgg aatattccca ggctacgcgc actgttatcc agcatgatga
    10921 tttctgcttc tgtttgcgaa acgagatcgc gctcatcatc ccagtcaggc atgacacggt
    10981 gggttaaatc ataaacgcgc tggaagttgc gccgttcaat caccatcacc tttccggcag
    11041 taaataaacc ttccagatgc cgtttatgcg gcttccattc ccaccagccg cttgcacctt
    11101 tacgaggatg ctcaaaatcg gctgaacgta ccggcccctt atcatgaata tgctgaatta
    11161 actgtgcaat ttccgcctca tgttcctgca tccaggcgtc tttgtatttc cagcccattt
    11221 tttcaggtgc cagcatgcgg tggcgaataa gacgaaagtc gctacgcggc ataaagcagg
    11281 cttcatgcgc ccagtattcc attaattcgc cacgcgccag agactcatcc agccactggg
    11341 caggataatt tcccagacga ctgaaaagca ccagatatgg actacgggca acaatattga
    11401 tggtatcgat ttgcagcaag gacatgcggg agatcgttgc cggaatatcc tccaacgacg
    11461 ctcgacggcg gggtttgttt aacaggcctt gtgcggcaag gtgaagatta cgcgcatcag
    11521 caagggagag gtgcggcagc gacattcatg actccatcaa tcgaacgctg ccgcggcgta
    11581 actagttgcc agaagccagc gaggttagtt gcgtaagcag tttcgctggt tcatcacctg
    11641 aaagctgtgc gtctacaggc aaataccacc aattttcttc tgcaaaggcc cggcatttca
    11701 ccgcatcttt ttcagtcatt accagcgttt gcccggcgct taccaacgca ctgacatccg
    11761 catggttcaa agactgatga tcggccagcg gtacacattt ttccggttgt acgccacaca
    11821 tcttcagcgt ggcaaaaaag cgcggcggat gcccaatccc cgccatcgcc actacatgtt
    11881 caagctgagc aacgtcacaa cgcgtaccgg tacgtaaatt caccgcctga cccggcagca
    11941 gatgcatggg gatttcaccg ctgcgaggga caccgccgtt gacgattacc gcatcaaccg
    12001 actttaagcg ccccgctcgc tcacgcattg gccccgccgg caaccaccag ccattgccaa
    12061 agcgacgcac accatcaata acgacaattt ccacatcacg cgccagacgg taatgctgta
    12121 aaccgtcgtc ggttacgatg atctgcacat cagggtgttg cgccagaatg gcttttaccg
    12181 catcagaacg aacgggagaa accgcaacag gcgcatcagt gcgttgataa atcaacacag
    12241 gttcatcacc cgcctgtgct gttgtggtat ctgccgacaa taacagcgga taagattcag
    12301 ccttactacc atatccccgc gatacgaccc ccacgcgaat accgcgctgt tgcaactgtt
    12361 ccaccagcca gacaacgacc ggggtttttc cgttgccgcc tgcggtgaga ttaccaacca
    12421 cgacaaccgg tacgggggca cgccaggcgc gcttcagttt tagtttatag caaagacgga
    12481 tcgcgccact caccaggcca tacaaccagg agagtggcag caatagccgc cacaaagggg
    12541 attcaccaga ccagattttt tcgatcattg gccaaactgc attttgtgaa gttgcgcgta
    12601 aacgccgcgg tgctcaagca aatcgttatg cgtaccgcgt tccacaatga caccatcctc
    12661 gacgaccacg atttcgtctg ccttttcaat ggtagacaag cggtgggcaa tcaccagaga
    12721 ggtacggttt ttctgcaact catccagtgc cgcctgaatc gcacgttcgg attcggtatc
    12781 cagagccgag gtagcttcat ccagaatcag aatcgggcta tcacgcagca aggctcgagc
    12841 gatagcaata cgctgacgct gaccgccaga gagcagcacg ccgttttcac caatcactgt
    12901 atcgagaccg ttatccatct tattgatgaa gtccatggcg taggccatac gcgccgcttc
    12961 ttcaatttgc tcacggctgt actgttcagt ccgtgcgtaa gcaatgttgt tagcaaccgt
    13021 atcgttaaac agatggacat tctgcgacac cagagcaacc tggttacgta acgacgccag
    13081 ggtatactcg cgcagatcgt gaccatccat caggatttcg ccttcatcaa tatcgtaaaa
    13141 acgagtgatc aggctggcga tggttgattt acctgaacca gagcgtccaa ccagagcaac
    13201 cgtcttccct gccggaattt tcaggttgat gttacgcaat gcaggtacgt cacgtcccgg
    13261 ataagtaaag gtgacattgc ggaattccac gtcgccagtc gcacgctcga tcacgcgctt
    13321 accttcgtct ttctcctgct cactgtccag aatggtaaac agcgtctgac aagccgccat
    13381 accgcgctgg aactgggcgt taacgttggt cagtgatttc agcggacgca tcagtgcaat
    13441 cattgaagag aaaacaacgg taatcgtacc ggcagtcagg ctatccatga cacttgggaa
    13501 gctcgccgca tacagaacaa acgccagcgc caaagaggcg atcagctgaa tgatcggatc
    13561 agagatggaa gaggctgaaa ccattttcat cccctgaaga cgcattcggt tgctgacttt
    13621 atcaaagcgt ttcgtttcca cttcctgacc accgaaaatc aatacttctt tgtggccctt
    13681 cagcatttgt tcggcgctgg tggtcacctg ccccatggtg ttctgcatgt ttttactgat
    13741 gttgcgaaaa cgcttcgata ctacgcgaat cgcaatcgaa acaatcggtg ccagcacaat
    13801 caaaatgatc gacagttgcc aactgtaata gaacatcatg atgaacaggc cgatgattga
    13861 cgcaccttca cgcacaaccg ttatcagtgc gccggaagaa gaagaagcaa cctgttcgga
    13921 atcgtaggtg atacgtgaca acagcgtccc cgttgactgt ttgtcaaaaa atgaaaccgg
    13981 cattcccatc atgtgaccaa acaggcggcg acgcatggtc attaccacct ttcctgatac
    14041 ccaggagata cagtagctgg agacatagct ggtgatacca cgtaaaatca tcagcccgat
    14101 caccaccagc ggcatccaca ccagcacgga gcgatctgtt ttaccaaagc catcatcaag
    14161 aagtggctta aggagcgata acatgaaggt atcgctggct gcgttgagga ttaacgctac
    14221 gcccgccacg atcagacccg ctttaaaagg cgcaatggtt ggccacagtc ggcggaacgt
    14281 ctgccacgta gagagatctt tgtcgttatg cattcaaaaa accagcattt gttgaaatag
    14341 ccgcatattc tacccgttat ccactggcac gccaaaccac tgatgatacc aacgaggtaa
    14401 aatttgctct ctgaggctgc taatccgcca gccttgcgct gaaaaattga ccgtcacttg
    14461 accctgatgt ggagtatcaa gccattgata tccttgctgt tgatagcgat gcttaacttt
    14521 attagagggc aatcgccatg cgttatagcg cgatgccgat gcgagtgcca cttttccatt
    14581 cactcgctga attaatggca acgatgatga ggtattactg ccatggtgag gtacctgaag
    14641 caatgttgtc tgcacttgct gccagtaacg gcttagcatc ttttgttcag cggggacttc
    14701 aatatcaccg gttagaagaa tgctattcgt cccgtcataa accttaacca cacaggaatg
    14761 gttatttcct ttatcgttgc tagcttgtaa aggccagtgc acgctgaaac gcaatccttg
    14821 ccattgccac gcttcgccac gcacacaggg ctgatgatgt tcccagttta acggacttct
    14881 gatccataac atcggccatg tgtgcaatat tgagtccagc cctccccggt gatccagatg
    14941 ttcatgactc agaataacgc cttccggttc aagattatgc cagtggagcc aggggataat
    15001 cagttgttgc ccactatccc cttcgggcca ggccagtcct gtgtcataga gaatcgcttt
    15061 gccgtttctg gctatcacca ttgccagccc ttgcccgaca tcaagcatgt acacctgcca
    15121 ctcgttaggt cgaggttttt gccacagcgg ccagcacatc agcaagccta cagccacaca
    15181 cattgctggc aacgttcgcc aggcgtttaa tcgccatacc accagtaaga accatgggga
    15241 aaatgatagc cattgccaac gttcagcaat gttgatccac ccttctggca aactctttaa
    15301 cccccagaaa agtaaagcca aagaccggtc ggcaagaaac cataaccctt gctccaggat
    15361 taacggcccg cttaaatgca caaccatcgc ggcgaggatc aacggaaccg tgataaatgt
    15421 caccaaggga attgctaata gatttgcaat aaacgaggtc agactaatgc catgaaatat
    15481 aacgatttgc acaggcataa gcaggagtgt gattcccagt tgcaaatgaa tgagggaaac
    15541 aactgcacgc aataccggcg gcagttgcca ctcaggacag ggaaaccact ggtaccaaaa
    15601 aatcagtgcc gcgaccgcag cggcagagag ccataaactt tgcgagagaa tggcaacagg
    15661 atccatcagc aaaattgccg ccagacaaca tatccataca tcccagccac tccactgtcg
    15721 cccacttaac ttaagcattc cccacgtagc aagcgccacc acggtacgca atgcaggagg
    15781 ttgcattccc gtcaaccagg cataaaaagc agcacagcag attccaccaa ttaatggcat
    15841 ttgccagtgg atccagcgcc caggcagaaa aacttgccca ccgcgaatga gtccggcagc
    15901 cagcaacgcc gcaaaagcga tgtgcaatcc cgatatcgcc attaaatgcg ccgttccagt
    15961 atcacgcatg atatttttga tttctttggg gacggataac cgttccccca tacctaaacc
    16021 aagaataacc gcattccaca tatagggttg cagcgttgtt tgtagtgacg ccagatactg
    16081 tgcacgcagg ctacaattcg gttcaatgac acttgcctgc agaaagcggc cgatgagcgg
    16141 ctgatgctgg gcaatggcat aacgctggct atcaaagccg ccatcattaa gttggccatg
    16201 aactgcacga actttgagtt tcattgacca ttgttgtcca gcacaaaccg cttgcggaag
    16261 atattcgcca tacagcacaa ggcctggcgc agggaatata cgtcgacctt gtagatgagt
    16321 aatttgaccg taatgagtgg tcatgccgtc agttgcagtg atctcaacaa ttgcatcctg
    16381 cgtcgcgcca gttaaggttt ctcctgccca caaaatttgc tttgctgata atatgcccca
    16441 aacgaaaaac agcaacgtca gcgcgatata acggacggtt ttaacaggga taaatgccag
    16501 tacgcaggcg aagagagtca gaaacgcaag ggttaatgtc ccaggcaatt ggggcaaaat
    16561 cagcaacgga aaaattccgc aaattatgca tacgccgact gtcgttattt tcatgcaccc
    16621 ctccctgttt atggaaagtg tgcaactttg ttgagcggtt gtctggtggg aattttgcgt
    16681 tccccgaggc acattcagaa gaatttaacg aaagggtgaa aactgctcaa aactctgcga
    16741 ggcagcttcc agttcctgat tagagaaatg aaaaaagcac ccgataggtg cttttctcgc
    16801 gttcaagttt gagtaaaaac ttaaccgtaa atattggcgc gatcgcgcag ttctttacca
    16861 ggtttaaagt gaggaacgta ttttccttcc agttctactt tatcgccagt cttcggatta
    16921 cgtccggtac gtggtgcgcg gtagtgcaaa gagaaactgc cgaaaccgcg gatttcaata
    16981 cgctcgccct gcgcaagagt cgaggccata tgctccagca tctcttttac tgcatcttca
    17041 accgtcttgg cgggaatgtg cgattgctgg gtggcaagtc tttctatcaa ttctgacttg
    17101 gtcatgattc ctccggttcc ttaaaggcaa attaagcggc tgctgttgct gcattgatta
    17161 agggcggccg tagccgccct tagtgcttga ttacaggacg aaacctgcaa tctgtcaagt
    17221 aaactcaaca aacttcggaa taaaaatccc gaagagtcag agaattactc gcctttagct
    17281 gctttgaaag cttcagccat tgcgttgttg gagaagtttg catcttcctg tttgttaaca
    17341 gttgcgattg catctttctc gtcagcttcg tctttcgcac gaacagacag gctgattgcg
    17401 cggtttttac gatcaacgcc ggtgaattta gcttcaactt cgtcgccaac gctcagaacc
    17461 agggtagcgt cttcaacgcg gtcacgggat gcttcagaag cacgcaggta accttcgacg
    17521 ccgtcagcca gttctacggt tgcgccttta gcgtcaactg cagttacttt accggttacg
    17581 atagcgcctt tcttgttcag agcaacccag ttgttgaacg gatcttctgc gagctgttta
    17641 acgcccaggg agatacgttc acgttctgcg tcaacctgca gaacaactgc agcgatttcg
    17701 tcgccttttt tgtattcacg aactgcttct tcgcctgcaa cgttccagga gatatcagac
    17761 aggtgaacca ggccgtcgat gccgccgtcc aggccgatga agataccgaa gtcagtgata
    17821 gacttgattt taccttcaac acggtcgccc ttgttgtggg tttccgcgaa ctgctgccac
    17881 gggttagctt tgcactgttt cagacccagg gagatacgac gacgttcttc gtcgatatcc
    17941 agaaccataa cttccactac atcgccaacg ttaacaactt tggacgggtg gatgtttttg
    18001 ttggtccaat ccatttcgga aacgtgtacc aggccttcaa cgccttcttc gatttcaacg
    18061 aagcagccgt agtcggtcag gttggtcacg cgaccagtca gtttggtacc ttccggataa
    18121 cgtttagcga tagctaccca cggatcttcg cccaactgtt tcaggcccag ggatacacgg
    18181 gtacgttcgc ggtcgaactt cagcacttta acagtgattt cgtcgcccac gttgacgatt
    18241 tcgctcggat gcttaacgcg tttccaggcc atgtcagtga tgtgcagcag gccgtcaacg
    18301 ccgcccagat caacgaatgc accgtagtca gtgaggttct taacgatacc tttaacttcc
    18361 atgccttcct gcaggttttc cagcagctga tcgcgctctg cgctgttttc ggattcgata
    18421 acggcacgac gagaaacaac aacgttgttg cgcttctgat ccagcttgat tactttaaat
    18481 tcaagctctt tgccttccag gtgcagagtg tcacgcaccg gacgaacgtc taccagagaa
    18541 cctggcagga acgcacgaat accgttcagc tcaacagtga agccgccctt aactttgccg
    18601 ttgataacac cggtaacagt ttcagcatct tcgtaagctt tttccagcgt gatccaggct
    18661 tcgtgacgtt tagctttctc acgggacagc agagtttcac cgaagccgtc ttctactgcg
    18721 tccagagcaa cgtcaacttc gtcacctacc tggatttcca gctcgccctg ggcgtttttg
    18781 aactgctcag ccgggatggc ggactcagat ttcagaccag cgtcaaccag tactacgtct
    18841 ttgtcgatag caacaacaac gccacgaacg atagaacccg ggcgggtttc gatttctttt
    18901 aaggactctt caaagagttg agcaaaagat tcagtcatgt ttaatcttca ggtttatatt
    18961 taacgtccac ctggctccat gccggatggg gttgtttaac atacccgctg tcattccatt
    19021 gcaacggggg tactgcaaat tcggtcgctt atgcgagagc caatttctgg cgcgcgtatt
    19081 gtagcgcttt ttcaatcact tgctcaatgc ttaaggtggt ggaatccaac actaaagcat
    19141 cggctgccgg aaccagtggc gctaccgctc ggttacgatc gcggtcgtcg cgttctttga
    19201 tctcggccaa aaggcgctca aagttaacac taaagccctt ctcctgcaac tgtagcatgc
    19261 ggcgatgcgc acgttcttcc gaggaggcgt caaggaaaat tttcactggt gcatcaggga
    19321 ataccaccgt tcccatgtcg cggccatcgg caatcagacc tggtaattcg cgaaacgcgc
    19381 gttggcgacg caataatgct tcacgaacgc gtgggaatgc cgcgacttgt gaagccgcat
    19441 tcgccacttc ctgagtacga atttcgccgc tgacatcttc cccttcgagg atcacttcca
    19501 gattgccatt ggttgacaca aaacgtacat ccagatggga tgccagcggt accagcgcat
    19561 cttctgacgc aacatcaaca tggtgatgta atgccgccaa tgccagtacg cgataaattg
    19621 cacccgaatc cagcagatgc cattgcaatg cttccgccat agccttacac aaggtgcctt
    19681 tccctgcacc gcttgggcca tcaatggtaa taaccggggc aattgccgtc atctttatct
    19741 ccttaaaaca ggcgtaccgt taacataacc gcgcgcatta tacgcgccga cgccacaaat
    19801 cgttaacgtt gtgtgcaaaa accagttttg tctgtattca gatggggatt tattgcgcaa
    19861 ttatagcggg ctggcagaag accagcccga aagatgacaa tgatttactt accagaggcg
    19921 atacgatcac ggatgtgttg cgcacgctct gttgatggtg ggtgagagtc aaacatggac
    19981 tgggtgcgac cgccatccag actagccagt ttttcaaagc tgccaaccag cccctgggtg
    20041 ctgatgccac gtttcttcaa cagatcaaag gagaaatcat ctgcttcgga ctctttatca
    20101 cgggagtatt tagcattaac agcgccttct gcgatatcac ccaattgtga gcgggaaagc
    20161 tgggaagcca caccgctggt agctgaaatg gcatcgcgtg cggcaacgat cgcataagaa
    20221 gctttcattt cagccagcga gtgacccaac gcgacgtggc ccagttcatg gcccagaaca
    20281 ccttcaattt cgttgtcatt catcatgttc atcaggccac tgtagacacg gacacagccg
    20341 ttcgccatcg cccatgcgtt gacgtcgctg gtcatataaa ccttatagtt gaccggcgtg
    20401 ccattaatat tgttacccaa tgctttggcg atttttgcca gacgtttacc gtacttgctg
    20461 gatttacttg cgacttgatt gccgctgtcc atttcggcac agccctgatt ggcaatcgct
    20521 tttgcatcgg catcgcttaa tgttgctgct ttataggcat ttaaaccgga gctgatagcc
    20581 atattggtat caataccgtg ggtattttga caccctgtca gtaatgctgc agaggtcgca
    20641 atcgccagca gtaatttagt attcttcatg tgaatcatcc gttgattaat catacataaa
    20701 cattatatat caacaactta cattaacaag cgacgctacc attaatcaaa atgactcaca
    20761 aggtccgaaa aaaaacgccg acaatgtgcc gacgttttga taaagaaaga tttggctatt
    20821 tattgcccgt tgttcattca ggctgcctgg ctaatccgcg ccaactgctc gaaataatcc
    20881 ggaaatgttt tggccgtaca tttgggatca agaatcgtca ctggtgtatc tgacaacgcc
    20941 accagcgaga aacacatcgc catccggtga tcattgtatg tcgcgatctc ggcaaagttc
    21001 agtttttccg gtggagtgat acgaatgtaa tcgtgcccct cttctacttc cgcaccgact
    21061 ttacgcagtt ctgttgccat cgcaaacagg cgatcggttt ctttaacacg ccagttatag
    21121 atattgcgca gcgtggtggt gccttttgca aataacgccg ccgtggcaat ggtcatcgcc
    21181 gcatcgggaa tatggttcat atccatatca atagcgttca gttcaccacg cgtgcaggaa
    21241 atataatcat cgccccagca aatggtcgcg cccatttttt ccagcacatc agcaaagcga
    21301 atatcaccct gcatactgtt acgtccaata ccggtcactt ttacagtgcc gcctttgatt
    21361 gctgctgctg ccagaaagta agaagccgaa gatgcatcgc cttcgaccaa ataagtaccc
    21421 ggagactgat aagactgtcc gccttttacg acaaattgtt gatagtgctg attttcaatt
    21481 tcaacaccaa acgtcttcat cagattgagt gtgatgtcga tataaggttt agaaaccaga
    21541 tcgcctttaa tacgaatcac cgtatcttct ggcgcaagag gcgcagtcat taacagtgcg
    21601 gtgaggaatt ggctggaaac ggagccatca acgtcaacgt tgccgccagt aaagccgccc
    21661 tgtaaacgca acggcggata attttcttgt tccaggtaag tgatcttcgc cccgcccagg
    21721 cgcagcgcat ccaccagatg accaatcggg cgttctttca tacgcggctc accggtcagc
    21781 acaatatcat tgctacccag acaaagagct gccgccagcg gacgcattgc cgttccggcg
    21841 ttaccgagga acaactccag ggcaccttct gcgtgtaatg gaccgccgtt accgataatt
    21901 tcgcaacgcg tacgatcggc tgaaagcgta tagcttaccc ctaacgctgt taatgcattc
    21961 agcatatggc gcacgtcatc gctatccagc agattggtta atactgtttt gccgtgtgct
    22021 aatgccgcca gcaataaagc gcggttagaa acgctcttgg aaccgggcag attaatagtg
    22081 ccatcgacac gagcgatggg ttgtaacgtc agggattcca tgaactcaac tctctacaac
    22141 agaaataaaa accccacagg ctggctgtgg ggattaagca aaatttcggc attaaccgtg
    22201 acggcgttcg aactcaacca tgaagtctgt cagcgcttta acgccttcca gcggcatggc
    22261 gttataaata gaagcgcgca ttccgccgac cacacggtga cctttcagtg catgaaggcc
    22321 agcagcaaaa gactcttcaa ggaacaattt gtcaagcgca ctgtccgcca actggaacgg
    22381 cacgttcatc cgcgaacggt tagctttcgc cacgtcattg cggtagaaat cgctgttatc
    22441 aatcacccca tatagcagtt ctgctttttg ctgattgatt ttatccattt cagctacacc
    22501 gccgttcgct ttcagccatt taaagaccag accagataaa taccaggcaa atgtcggcgg
    22561 cgtgttaaac atggagccgt tatcgttgaa gatggaataa tccagtatcg acggacacgc
    22621 gatattcgct ttgcccagca aatcttcacg aacgatgacg attgtcaggc cagccgggcc
    22681 gatatttttc tgcgcgccag cgtaaatcac gccataacgg ctgacgtcaa tcggacggga
    22741 aagaatggtt gaagagaagt cggcggcgac caccacatct ttgccgaagt ctggcgtttc
    22801 gtcgatggcg ataccgtcga tggtttcatt cgggcagtaa tgcatataag cagcattatc
    22861 agagagttgc cattcacgca ttggcttaac cgcgcgcaga ccatcaacag tcactttggc
    22921 gtcaaagaca ttaggcgtgc agtatttttt cgcttcttta atggcacttg ccgcccagta
    22981 accggcatca acataatctg cggtggtttt atcaccgaga atattcagcg gtaccgcagc
    23041 aaactgaccg cgaccaccgc catggcagaa taataccttg tagttggagg ggacattaag
    23101 aagatcgcga aaatccttct cggcttcctc tgcaacctga atgaactctt tgccacggtg
    23161 actcacttcc atcaccgacg taccaagacc gttccagtcg cgcagttcct gttgagcctg
    23221 tttaagcacc tctgccggta gcattgccgg accagaacta aaattgaaga tttgagccat
    23281 ttcccctcac cacgttgcgt tgctattaac ccgcggtcaa tcgattgcga ccacaggtaa
    23341 tacattattc ctgtggctat cggttttatc attcagtgac acacatcgca atgagtaaaa
    23401 ccgttgaggg caaaaatgtg gcttgcatca cacaaaagaa tctctcggat tattagcaaa
    23461 aagccgatgt ttttactgtc aggtcacccg ttttgcccga cttgccttcg tccattctgg
    23521 atttgcacag cgcggacata attgtttttc ccccgctttc atatgaatga tttcactaca
    23581 acgaatacag gcatagtccg ccgactccgg caccactttg taacgctgcg tacaatcact
    23641 gggtaaaata agtaaacccg gtttcacctt gtcatcttca aagaaataga cactgatttg
    23701 cccgttggtt tcgagaatcg ccagacgtac ctgccccagc tgctccacgc cacgcaatcg
    23761 tagctccata aagaactcaa attccgtcat gttggagtta ttgagtttcg accaggccag
    23821 ctcgccatct tcaataataa caaccggctt accttccagt agatcttcca gtttttcact
    23881 gtgcgccatc aaccacatta ccaggcggta taacaacgcc agagtgataa agacgataag
    23941 taccggcacc atcggcacat catcataaaa cgccacatct cccgccgctg aacccagcgt
    24001 gagaatgatt aacacttcaa acagcgacat ctgtcgcaca ccgcgtcttc cggtcatttt
    24061 gaggaacaaa aaaaccagta caaaggtata aagactacgt agtgcaactt cgccaaggaa
    24121 atcaaaaggc actttatcaa atgccatacg gtggagatcg aacgccttca tttttatgct
    24181 cttatcgtgg tttattcgct aaaagcatag tcaagagttc atcggcatgg cggcggtgat
    24241 agcgcctttg agataaaacc cgtattattg cgcgctttcc gtacgactaa agtgattttc
    24301 atgacgcaaa catttatccc cggcaaagat gccgctctgg aagattccat cgctcgcttc
    24361 cagcaaaaac tttcagacct cggctttcag attgaagagg cctcctggct gaatcctgtg
    24421 cctaacgtct ggtctgtaca tattcgcgac aaagagtgcg cactgtgttt taccaacggt
    24481 aaaggcgcaa ccaaaaaagc ggcgctggct tctgcacttg gtgaatattt cgagcgtctc
    24541 tcaaccaact acttttttgc tgatttctgg ctgggcgaaa ccatcgccaa cggtccgttc
    24601 gtgcattatc ccaacgaaaa atggttccca ctgaccgaaa atgacgatgt gccagaagga
    24661 ctactcgatg accgtctgcg cgcgttttat gatccggaga atgaactgac cggcagcatg
    24721 ctgattgacc tacaatccgg taacgaagat cgtggtattt gcggcctgcc gtttacgcgt
    24781 cagtccgaca atcagaccgt ttatattccg atgaatatca ttggtaacct gtacgtctcc
    24841 aacggtatgt ccgcaggtaa tacccgcaac gaagcacgcg ttcagggatt gtctgaagtt
    24901 ttcgaacgct acgtgaaaaa ccgcattatt gctgaaagca tcagcctgcc agagatcccg
    24961 gcagacgtgc tggcgcgtta cccagcggtg gttgaagcga tcgaaacact ggaagcagaa
    25021 ggtttcccga tcttcgcata tgatggttcc cttggcggcc agtatccggt gatttgcgtg
    25081 gtactgttca atcctgctaa cggtacctgc tttgcctctt tcggtgcgca tcctgatttt
    25141 ggcgtagcac tggaacgtac cgtgaccgag ctgctgcaag gtcgtggcct gaaagatttg
    25201 gatgtgttta ctccgccaac cttcgatgat gaagaagtcg ctgaacatac caacctcgaa
    25261 acgcacttta tcgattccag cggtttaatc tcctgggacc tgttcaagca ggatgccgat
    25321 tatccgtttg tggactggaa tttctccggc accacggaag aagagtttgc tacgctgatg
    25381 gctatcttca acaaagaaga taaagaagtt tatattgccg attacgagca tctgggcgtt
    25441 tatgcttgcc gtattatcgt gcctggcatg tccgatattt atccggctga agatctgtgg
    25501 ctcgcgaata acagtatggg cagccattta cgtgaaacga ttctttcgct accaggcagc
    25561 gagtgggaaa aagaagatta cctgaacctc atcgagcaac tggatgaaga aggttttgat
    25621 gactttaccc gcgtgcgtga gctgttgggt ctggcgaccg ggtcggataa cggttggtac
    25681 accctgcgta tcggtgaatt aaaagccatg ctggcgctgg ctggtggcga tctggaacag
    25741 gctctggtct ggaccgaatg gacgatggag tttaactcat cagtatttag tccggaacgc
    25801 gccaactatt atcgctgcct gcaaacgttg ttattactgg cacaggaaga agatcgccag
    25861 ccgctgcaat atctgaatgc gtttgttcgc atgtacggcg cagatgccgt ggaagccgcc
    25921 agtgcggcaa tgagcggcga agcggcgttt tacggcctgc aaccggtaga tagcgatctg
    25981 cacgcgtttg ctgcacatca gtcgttgttg aaggcctacg aaaagctgca gcgcgccaaa
    26041 gcagcattct gggcaaaata aaatcaaata gcctacgcaa tgtaggctta atgattagtc
    26101 tgagttatat tacggggcgt ttttttaatg ccccgcttta catatatttg cattaataaa
    26161 ataattgtaa ttataaggtt aaatatcggt aatttgtatt taataaatac gatcgatatt
    26221 gttactttat tcgcctgatg ctccctttta attaactatt ttagcggagg atgcggaaaa
    26281 aattcaactc atttgttaat ttttaaaatt tatttttatt tggataatca aatatttact
    26341 ccgtatttgc ataaaaacaa tgcgagttac gggcctataa gccaggcgag atatgatcta
    26401 tatcaatttc tcatctataa tgctttgtta gtatctcgtc gccgacttaa taaagagaga
    26461 gttagtgtga aagctgacaa cccttttgat cttttacttc ctgctgcaat ggccaaagtg
    26521 gccgaagagg cgggtgtcta taaagcaacg aaacatccgc ttaagacttt ctatctggcg
    26581 attaccgccg gtgttttcat ctcaatcgca ttcgtcttct atatcacagc aaccactggc
    26641 acaggcacaa tgcccttcgg catggcaaaa ctggttggcg gcatttgctt ctctctgggg
    26701 ctgattcttt gtgttgtctg cggagccgat ctctttactt ccaccgtgtt gattgttgtt
    26761 gctaaggcga gtgggcgcat cacctggggt cagttggcga aaaactggct aaatgtctat
    26821 tttggcaacc tggtcggcgc actgctgttt gtacttttaa tgtggctttc cggcgagtat
    26881 atgaccgcaa atggtcaatg gggactaaac gtcctacaaa ccgccgacca caaagtgcac
    26941 catactttta ttgaggccgt ctgtcttggt atcctggcaa acctgatggt atgtctggca
    27001 gtatggatga gttattctgg ccgcagcctg atggacaaag cgttcattat ggtgctgccg
    27061 gtcgcgatgt ttgttgccag cggttttgag cacagtatcg caaacatgtt tatgatcccg
    27121 atgggtattg taatccgcga cttcgcatct ccggaattct ggaccgctgt cggttctgca
    27181 ccggaaaatt tttctcacct gaccgtgatg aacttcatca ctgataacct gatcccggtt
    27241 acgatcggta atattatcgg cggtggtttg ttggttgggt tgacatactg ggtcatttac
    27301 ctgcgtgaaa acgatcacca ttaatggttg tcgaagtacg cagtaaataa aaaatccact
    27361 taagaaggta ggtgttacat gtccgagctt aatgaaaagt tagccacagc ctgggaaggt
    27421 tttaccaaag gtgactggca gaatgaagta aacgtccgtg acttcattca gaaaaactac
    27481 actccgtacg agggtgacga gtccttccta gctggcgcta ctgaagcgac caccaccctg
    27541 tgggacaaag taatggaagg cgttaaactg gaaaaccgca ctcacgcgcc agttgacttt
    27601 gacaccgctg ttgcttccac catcacctct cacgacgctg gctacatcaa caagcagctt
    27661 gagaaaatcg ttggtctgca gactgaagct ccgctgaaac gtgctcttat cccgttcggt
    27721 ggtatcaaaa tgatcgaagg ttcctgcaaa gcgtacaacc gcgaactgga tccgatgatc
    27781 aaaaaaatct tcactgaata ccgtaaaact cacaaccagg gcgtgttcga cgtttacact
    27841 ccggacatcc tgcgttgccg taaatctggt gttctgaccg gtctgccaga tgcatatggc
    27901 cgtggccgta tcatcggtga ctaccgtcgc gttgcgctgt acggtatcga ctacctgatg
    27961 aaagacaaac tggcacagtt cacttctctg caggctgatc tggaaaacgg cgtaaacctg
    28021 gaacagacta tccgtctgcg cgaagaaatc gctgaacagc accgcgctct gggtcagatg
    28081 aaagaaatgg ctgcgaaata cggctacgac atctctggtc cggctaccaa cgctcaggaa
    28141 gctatccagt ggacttactt cggctacctg gctgctgtta agtctcagaa cggtgctgca
    28201 atgtccttcg gtcgtacctc caccttcctg gatgtgtaca tcgaacgtga cctgaaagct
    28261 ggcaagatca ccgaacaaga agcgcaggaa atggttgacc acctggtcat gaaactgcgt
    28321 atggttcgct tcctgcgtac tccggaatac gatgaactgt tctctggcga cccgatctgg
    28381 gcaaccgaat ctatcggtgg tatgggcctc gacggtcgta ccctggttac caaaaacagc
    28441 ttccgtttcc tgaacaccct gtacaccatg ggtccgtctc cggaaccgaa catgaccatt
    28501 ctgtggtctg aaaaactgcc gctgaacttc aagaaattcg ccgctaaagt gtccatcgac
    28561 acctcttctc tgcagtatga gaacgatgac ctgatgcgtc cggacttcaa caacgatgac
    28621 tatgctatcg cttgctgcgt aagcccgatg atcgttggta aacaaatgca gttcttcggt
    28681 gcgcgtgcaa acctggcgaa aaccatgctg tacgcaatca acggcggcgt tgacgaaaaa
    28741 ctgaaaatgc aggttggtcc gaagtctgaa ccgatcaaag gcgatctcct gaactacgat
    28801 gaagtgatgg agcgcatgga tcacttcatg gactggctgg ctaaacagta catcactgca
    28861 ctgaacatca tccactacat gcacgacaag tacagctacg aagcctctct gatggcgctg
    28921 cacgaccgtg acgttatccg caccatggcg tgtggtatcg ctggtctgtc cgttgctgct
    28981 gactccctgt ctgcaatcaa atatgcgaaa gttaaaccga ttcgtgacga agacggtctg
    29041 gctatcgact tcgaaatcga aggcgaatac ccgcagtttg gtaacaatga tccgcgtgta
    29101 gatgacctgg ctgttgacct ggtagaacgt ttcatgaaga aaattcagaa actgcacacc
    29161 taccgtgacg ctatcccgac tcagtctgtt ctgaccatca cttctaacgt tgtgtatggt
    29221 aagaaaactg gtaacacccc agacggtcgt cgtgctggcg cgccgttcgg accgggtgct
    29281 aacccgatgc acggtcgtga ccagaaaggt gctgtagcgt ctctgacttc cgttgctaaa
    29341 ctgccgtttg cttacgctaa agatggtatc tcctacacct tctctatcgt tccgaacgca
    29401 ctgggtaaag acgacgaagt tcgtaagacc aacctggctg gtctgatgga tggttacttc
    29461 caccacgaag catccatcga aggtggtcag cacctgaacg ttaacgtgat gaaccgtgaa
    29521 atgctgctcg acgcgatgga aaacccggaa aaatatccgc agctgaccat ccgtgtatct
    29581 ggctacgcag tacgtttcaa ctcgctgact aaagaacagc agcaggacgt tattactcgt
    29641 accttcactc aatctatgta attagatttg actgaaatcg tacagtaaaa agcgtacaat
    29701 aaaggctcca cgaaagtggg gcctttttta gcacgagagc cttttttgtc agctatctat
    29761 actttaaggt gactgccaaa acagactcga cgtagccttc gagctgcgca ccaacacggc
    29821 ctcagatggg ccacatctgg agaaacaccg caatgtcagt tattggtcgc attcactcct
    29881 ttgaatcctg tggaaccgta gacggcccgg gtattcgctt tatcaccttt ttccagggct
    29941 gcctgatgcg ctgcctgtat tgtcataacc gcgacacctg ggacacgcat ggcggtaaag
    30001 aagttaccgt tgaagatttg atgaaggaag tggtgaccta tcgccacttt atgaacgctt
    30061 ccggcggcgg cgttaccgca tccggcggtg aagcaatcct gcaagctgag tttgttcgtg
    30121 actggttccg cgcctgcaaa aaagaaggca ttcatacctg tctggacacc aacggttttg
    30181 ttcgtcgtta cgatccggtg attgatgaac tgctggaagt aaccgacctg gtaatgctcg
    30241 atctcaaaca gatgaacgac gagatccacc aaaatctggt tggagtttcc aaccaccgca
    30301 cgctggagtt cgccaaatat ctggcgaaca aaaatgtgaa ggtgtggatc cgctacgttg
    30361 ttgtcccagg ctggtctgac gatgacgatt cagcgcatcg cctcggtgaa tttacccgtg
    30421 atatgggcaa cgttgagaaa atcgaactcc tcccctacca cgagctgggc aaacacaaat
    30481 gggtggcaat gggtgaagag tacaaactcg acggtgttaa accacctaag aaagagacca
    30541 tggaacgcgt gaaaggcatt cttgagcagt acggtcataa ggtaatgttc taatttcccc
    30601 gtaaagcggc aactttattg agttgccgct tttttattcc gcgcttcatt ctgctacagt
    30661 gtttttaaca cattttatag tgcatcta
//
LOCUS       DAJTMK010000031        29339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTMK010000031 DAJTMK010000000
VERSION     DAJTMK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29339
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.032"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(311..2002)
                     /gene="fruA"
                     /locus_tag="OO135_001193"
     CDS             complement(311..2002)
                     /gene="fruA"
                     /locus_tag="OO135_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5397118.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2019..2957)
                     /gene="fruK"
                     /locus_tag="OO135_001194"
     CDS             complement(2019..2957)
                     /gene="fruK"
                     /locus_tag="OO135_001194"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5397119.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2957..4087)
                     /gene="fruB"
                     /locus_tag="OO135_001195"
     CDS             complement(2957..4087)
                     /gene="fruB"
                     /locus_tag="OO135_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5397120.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4455..5636
                     /gene="setB"
                     /locus_tag="OO135_001196"
     CDS             4455..5636
                     /gene="setB"
                     /locus_tag="OO135_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5397121.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(5633..5887)
                     /locus_tag="OO135_001197"
     CDS             complement(5633..5887)
                     /locus_tag="OO135_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5397122.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6042..6614
                     /gene="yeiP"
                     /locus_tag="OO135_001198"
     CDS             6042..6614
                     /gene="yeiP"
                     /locus_tag="OO135_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5397123.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6837..8303
                     /locus_tag="OO135_001199"
     CDS             6837..8303
                     /locus_tag="OO135_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5397124.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8421..9407
                     /gene="yeiR"
                     /locus_tag="OO135_001200"
     CDS             8421..9407
                     /gene="yeiR"
                     /locus_tag="OO135_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5397125.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDHATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHIVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9446..10159
                     /gene="lpxT"
                     /locus_tag="OO135_001201"
     CDS             9446..10159
                     /gene="lpxT"
                     /locus_tag="OO135_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5397126.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVFFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10571..11137
                     /gene="mepS"
                     /locus_tag="OO135_001202"
     CDS             10571..11137
                     /gene="mepS"
                     /locus_tag="OO135_001202"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5397127.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11318..12874
                     /locus_tag="OO135_001203"
     CDS             11318..12874
                     /locus_tag="OO135_001203"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5397128.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12956..14770
                     /locus_tag="OO135_001204"
     CDS             12956..14770
                     /locus_tag="OO135_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5397129.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYNGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14771..15865
                     /locus_tag="OO135_001205"
     CDS             14771..15865
                     /locus_tag="OO135_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5397130.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15865..16890
                     /locus_tag="OO135_001206"
     CDS             15865..16890
                     /locus_tag="OO135_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5397131.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRNIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16892..18481
                     /gene="yejF"
                     /locus_tag="OO135_001207"
     CDS             16892..18481
                     /gene="yejF"
                     /locus_tag="OO135_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5397132.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQATKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKQQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18485..18829)
                     /locus_tag="OO135_001208"
     CDS             complement(18485..18829)
                     /locus_tag="OO135_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397133.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALNDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19162..20352)
                     /gene="bcr"
                     /locus_tag="OO135_001209"
                     /pseudo
     CDS             complement(19162..20352)
                     /gene="bcr"
                     /locus_tag="OO135_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20380..21075)
                     /gene="rsuA"
                     /locus_tag="OO135_001210"
     CDS             complement(20380..21075)
                     /gene="rsuA"
                     /locus_tag="OO135_001210"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5397134.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21224..22984
                     /locus_tag="OO135_001211"
     CDS             21224..22984
                     /locus_tag="OO135_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5397135.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFHFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23109..23393
                     /gene="rplY"
                     /locus_tag="OO135_001212"
     CDS             23109..23393
                     /gene="rplY"
                     /locus_tag="OO135_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5397136.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKAAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23532..24539)
                     /gene="yejK"
                     /locus_tag="OO135_001213"
     CDS             complement(23532..24539)
                     /gene="yejK"
                     /locus_tag="OO135_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5397137.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24721..24948
                     /locus_tag="OO135_001214"
     CDS             24721..24948
                     /locus_tag="OO135_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5397138.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRLAIANSFARALQSSINEDKAH"
     gene            24968..26728
                     /gene="pbgA"
                     /locus_tag="OO135_001215"
     CDS             24968..26728
                     /gene="pbgA"
                     /locus_tag="OO135_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5397139.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26803..26879
                     /locus_tag="OO135_001216"
     tRNA            26803..26879
                     /locus_tag="OO135_001216"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26837..26839,aa:Pro,seq:ggg)
     gene            complement(26982..>29339)
                     /gene="yejO"
                     /locus_tag="OO135_001217"
     CDS             complement(26982..>29339)
                     /gene="yejO"
                     /locus_tag="OO135_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5397140.1"
                     /translation="NGGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVY
                     GAANDPMIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVM
                     EAFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATG
                     VDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTIL
                     DEHVTMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGT
                     MVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSV
                     WAIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGN
                     GNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTL
                     GNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTV
                     RERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTI
                     RGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKM
                     SNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADV
                     GNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAG
                     TPGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         7073 a         7694 c         7183 g         7389 t
ORIGIN      
        1 ttttacgtaa agtaaattac cgccagagag gcaaaatggg cagatttgag caggctcaca
       61 aaatgcgatt aaatgcctga attacaaaag cgaggaattt gtgacgggat gcacagaacg
      121 ggaacaataa aaaaagcaga aaataatctg cccttgagga aataacctta gtatttgatg
      181 aacgtttcaa gtttctactg aaatataaaa ccatttaaat tcataaaatt acattcacaa
      241 accggtcatt ctctttgcgc tcatcaaatg ttacagggca ggaaatttct gccctgtaac
      301 acacctttta ttacgctgct ttcgctactg cgtccacttc cggacgtttc aggaaggcat
      361 aggccaaacc cgccaccagc gtaccagcaa taattgctac taggtaaccc agtaccggcg
      421 taatagcgcc agggatcagc agaacaaaca gaccaccgtg cggtgccatc agtttcgcac
      481 caatcgccat tgagattgcg ccagtcagcg ccccacccac gatacagcac ggcagcacac
      541 gcatcggatc acgagcagca aacggaattg caccttccga aatgaagcac agtcccaata
      601 ccagagcggc tttgccacct tcctgctgcg ctttgtcgaa tttgcgacgc gccaccattg
      661 ttgccagacc cattgccagc ggtggcacca tacctgccgc cataatcgcc gccatcgggc
      721 catatgtttg agtactcagc agacccacac cgaatgcgta cgctgctttg tttaccggac
      781 cgcccatgtc agtacacatc atgccaccga ggatcgcccc cagcagaacc gcattcgcag
      841 tccccatggt ctgcagccag tgagtcagcc cttcgagaat gccagcaact ggtttaccga
      901 tcaggtagat catcgccaga ccgaccacca gactggaaat tagcggaatg atcaggatcg
      961 gtttcagcgc ctccatactc tgtggcagtt tcagttgcgt actgattaac ttcgcaatgt
     1021 aaccagccag gaagccagca attataccgc caataaagcc agaaccggtg ctgaccgcca
     1081 gcataccgcc aatcagaccc ggcgtgaggc ccggacgatc ggcaatggag aaggcaatat
     1141 aacctgccag taccggcacc atcagcgcaa aggctgaacc accgccaatc tgcatcagcg
     1201 ccgctgccaa cgtacccggc tctttaaacg cttcgatacc aaaagcaaaa gaaagcgcga
     1261 tacacagacc acctgcgacg accatcggca gcatataaga gacgcctgtc agcaagtgac
     1321 ggtaagcgcc tgcactctct ttcttacctt cagtggtcgc cgtttgagct ttgcccgccg
     1381 gttcatacgg cgttgcttca gcaaccgctt tatccagttc ctgcgcggtt ttcttcagcg
     1441 ccagaccggt agaggtacga tacatcggtt taccagcaaa tttcgccaga tccacttcga
     1501 tatctgccgc cacaatcacc agatccgctg ctgcgacttc ttcaggggtg attgcattgc
     1561 ccgcgccaac agaaccacgg gtttcaactt tcacccacca gccacgtttt ttcgcttcgg
     1621 tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc agtcgggcaa gcagtcaccg
     1681 caactacgcg tttcggaccg ctggcagcaa ccggtgctgt cgcagcgacc ggcgcagtgt
     1741 aaggtttcgc atggcctttg gcttcactca ggaacagctc agggtgcgca actgcccggg
     1801 aaatatcacc cagccagaca tttttaccgt tcagcgcgct gtcattcggg atggaatcac
     1861 cgagaacaat cgccatttct gcgtcgttcg gattgtcgat gatttccagt tttgcttttc
     1921 gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg tgcctgaccg agattagcgt
     1981 caataatcag cagcgttttc attatgcctc tcctgctgtc agttaaaagg ttgtaagtcg
     2041 acgcgcgcca tcattgcggc caactgcgga cgatcggtaa tacccacatt gctttgactt
     2101 accgccaggg ctgcaacagc tgtcgccaga cgcagtgtgt gttcactgga ttcacgcatc
     2161 agcaagccat aaatcaggcc accaaccata gaatcccctg cgccaacggt gcttacgaca
     2221 tcgactgacg gtggtttggc gatccattcg ccggaggcat taacccaaag cgcgccttcg
     2281 gcacccagtg aaataacaac atgcgcaata ccttgttcac gtagcgcatg tgcagcttca
     2341 atcacatctt tcatttcagg cagtttacgg cctgcccaga tttccaactc gcggcggtta
     2401 ggtttcacca gccacggtgc cgctttcaaa cctgctacta acgcttcacg gctactatca
     2461 aagataatgc aaggacactg actacgcagg cgagtcatcc agtcggtgaa cgcttccggg
     2521 ctgacgcctg acggtaagct tccgctgaca cagaccatat cgaactgacc gagccagctc
     2581 agagaatcag tcacaaagcg ttcccagtcg gcgggggtga cttcaaaacc cgagaagttg
     2641 aagtcggtca cttcgccgtc tttttccgtc agcttaacgt taattcgggt gcgcccctgt
     2701 acaacctgga aacggttggc aatgcccagc tcgctgaaca gttgctgaaa accatcctga
     2761 ttgtctttac ccaggaagcc gccaacggtg acatcaattc ccaggtcttt taatactttg
     2821 gccacgttga tgcctttacc cgccgcatgc agaccggtgg ttttcaccag gttcacttcg
     2881 ccgcgttcaa tttccgggca gaaaccaaca aggtcataag ccggattaag ggtgatcgta
     2941 gcaacacgtc tgctcattat gcgccctccc caagaccagc agcgatagcg tcgccaatgg
     3001 ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa gcgtaggcga tgacctttct
     3061 taacgccaag tgccacaact ttcatcagac tacgtccgtt tgccggtttg ccggtgccat
     3121 caaggtttgt cacggtaata tcactgttaa attgtttaat ggtattgacc agcatggtac
     3181 ctggacgagc atgcaggccg tgttcattgc gcaccacaaa ctccgcgctt aacacgtcgt
     3241 cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt tgccgcatct gctttcagca
     3301 agcggtcagc tttattgtcg agcaataaat cagcgagacg cttaagaacc gcgatgggct
     3361 gatcgtcatt catcgccaca ctcaccagca tggctgccgt ttcgccgtcc acagcaaaag
     3421 catttgccgc acggcttacc gcaatcgcgc tacgcagatt gccttcggcg ctatcgctca
     3481 gccagatacc ctgtccgaga ttcagcggtt gttcattgat ggctttggtg acgaaagtgg
     3541 cgtcaactgc ccccgcctct ttcagacgcg cagcgttcag cgcctgaaga gtcagcagat
     3601 cgctggcgac gatatccagc gtcagcattt cgttgtcgag cttcagctgc tcactctgct
     3661 tttcgcccat cagtaatgcg cgaagttctt ctgctgttgt tgctgacttc agttgttcag
     3721 caacggaatc atcgctcagt acgtgggtca gctggcgtag caggcccaga tgttcatccg
     3781 agctggcagc aataccgatt gccacgtacg ctacctgacc gtcaccccag gtgacgcctt
     3841 ccgggaactg aaatacctga acgccggttt tcagcacctg atcgcgggtg tcggtagtgc
     3901 cgtgtggaat agcaatacca ttgccgagga acgttgaggt ttgctgttcg cgcgccagca
     3961 tgccattgac gtagccttct gctacattac cggcctgcac cagcgccgca gcgacctggc
     4021 gaatcgcctc ttctttgtct ccggcctttt cgcccggatg gatgtcctgt acggataact
     4081 ggaacatagt tctcctctct tgctgaattg aaacgattcg gcctctatga gaaaaaaagc
     4141 gccaacctgg cttagggtta aagacaagat cgcgctgaaa cgtttcaaga aagcataata
     4201 cttctgtttc agcacgcaag gaaagccgta aagttatgag caaaagtttg atgtgctgca
     4261 catttttttg tcatttttcc taattgctga tgggaaaatc cgttatcagc aatttcattt
     4321 cagcagcgtc agcagccaat cagcaacagt gaaatggcat tttgattatt cgtggcgctt
     4381 ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa actccgcgtc ttcctcttcc
     4441 agtgatagac ctgcatgcat aactcccccg cagtctccag cgcgaaatcg tttgacctga
     4501 cctcgacggc gtttttaatc gttgcctttc tcaccggtat tgcgggtgct ctgcaaaccc
     4561 cgacactcag tattttcctt accgatgaag tacatgcccg tccggcgatg gtgggattct
     4621 tctttaccgg cagcgctgtc attgggattc tggtaagtca gtttctcgct gggcgctctg
     4681 ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg cctgttaggc gtgctggcct
     4741 gcaccctttt tgcctggaat cgcaactact ttgttttgct attcgttggc gtctttctta
     4801 gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct tgcccgtgaa catgccgaca
     4861 aaaccggacg tgaggcggtg atgttcagct cttttttacg cgctcaggtt tcactggcat
     4921 gggtcattgg cccaccgctg gcttatgcgt tagcgatggg tttcagcttt acggtaatgt
     4981 atctgagcgc agcggtagcg tttattgttt gcggcgtgat ggtgtggctg tttttaccgt
     5041 cgatgcaaaa agagcttccg ctggcgaccg gcacggttga agcgccgcgc cgtaaccgtc
     5101 gcgatacgct actgctgttt gtcatttgta cattgatgtg gggctcgaac agcctgtaca
     5161 tcatcaacat gccgctattt attatcaacg aactgcatct tcccgagaaa ctggccggtg
     5221 tgatgatggg gaccgccgcc gggctggaaa tcccgacgat gttgattgcc ggatatttcg
     5281 ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc cgtgggtggc gtctgttttt
     5341 acgcaggaat gctgatggcg cattcacctg ccattctgtt gggcttgcag ctgctaaatg
     5401 ctatttttat tggcattctg ggcggcatcg ggatgctcta ttttcaggat ttgatgcccg
     5461 gtcaggcagg ttcagccacc acgctctata ccaacacgtc gcgcgtgggc tggatcatcg
     5521 caggatcagt ggcgggcatc gtcgccgaga tctggaatta tcacgctgtg ttctggtttg
     5581 cgatggtgat gattatcgcc actctgtttt gcttactgcg gattaaagat gtttaagacg
     5641 cggtcagcat ttccagatcg ataagccagg tcatggcctg ctgacgcgag ttaccgcaca
     5701 tttcagcgct ggcttgtaag cctgcacaga ctttcggtcg cagtggcgag gtgaaaattt
     5761 tacagcgttg ctgttcatca agctgaatgc agggcgtatt ggcgggcttg ccatctggca
     5821 taccgggaat agggctgaaa attgaagggg cagtgcaaca cgccccgcaa cccggacggc
     5881 attccatacg ttgttctctt aaggttaacc accggattga tgagcgcgca cagtaccacc
     5941 ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa aggcccgtcg ctagtacttt
     6001 gtcgcgatat ttttgacatt ttcgactaca ggaatttttc gatgccaaga gcgaacgaaa
     6061 ttaaaaaagg tatggtactg aattacaacg gcaaactgct gctggttaag gatattgata
     6121 ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa aatgcgtttt tctgatgtcc
     6181 gtaccgggct gaaagtagaa gagcgtttca aaggtgatga tatcgttgac accgtgacgc
     6241 tgacccgccg ttacgttgac ttctcctatg tcgatggcaa cgaatatgtc tttatggata
     6301 aagaagacta taccccgtat accttcacca aagatcagat tgaagaagag ttgctgttta
     6361 tgccagaggg cggcatgccg gacatgcagg tgctgacctg ggacggtcaa ctgctggcgc
     6421 ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac cgcaccaggc atcaaagggg
     6481 catctgccag cgcccgtaac aaaccggcga cattgagcac tggtctggtg attcaggtac
     6541 cagaatactt aagcccgggc gaaaaaattc gtatccatat cgaagaacgc cgttatatgg
     6601 ggcgtgctga ctaacttcag ccgcatgcag aaaagggata gctcaggctg tccctttttt
     6661 aatttattat accaatcttc tattttgcgc ttcctgaaca ccttacgccc tcaattttca
     6721 ctcgttgatt gatcgccctc acacttcatc gcattaacaa tccagaccaa tttcaattgc
     6781 tgtcatataa ctttacactg tcattgttaa tttatcgtta ctaagacgtg actcctatga
     6841 agacaattgc ctccgttacg ctcccgcatc atgtacacgc tccacgctac gatcgccagc
     6901 agttgcaatc acgtatcgtt cattttggct ttggcgcctt tcaccgcgct catcaggcgt
     6961 tactgaccga tcgtgtgctg aatgcccagg gcggcgactg ggggatctgt gaaatcagct
     7021 tgttcagcgg tgatcaactg atgagccagc tccgcgcaca gaaccattta tataccgtgc
     7081 tggagaaagg tgcggacggc aatcaggtga taattgtcgg tgccgttcac gaatgcctta
     7141 atgcaaaact ggattcctta gcggcaatta ttgagaaatt ttgcgagcca caggtggcaa
     7201 ttgtttccct gacgattacc gaaaaaggct attgtattga cccggccacc ggtgcactcg
     7261 acaccagtaa tccgcggatt attcacgatc tacaaacccc tgaagaacct cactccgcac
     7321 cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg cggccttaca ccgtttaccg
     7381 tgctctcctg cgacaatatt cccgacaatg gccatgtggt gaaaaacgcg gtgctgggaa
     7441 tggcagaaaa acgttcgcca gaactcgccg ggtggataaa agaacacgtc agttttccgg
     7501 gaaccatggt cgaccgcatt gttccggctg caaccgacga atcactggca gaaatcagcc
     7561 agcatctggg ggtaaatgat ccctgcgcga ttagctgcga accgtttatc cagtgggtgg
     7621 tggaagataa cttcgtcgct gggcgtcctg cctgggaagt cgcaggtgta caaatggtga
     7681 atgatgtcct gccatgggaa gagatgaaac tgcgtatgct taatggcagc cactcttttc
     7741 tcgcttatct gggttacctt tcaggattcg cccatatcag tgattgcatg caggatcgcg
     7801 catttcgcca tgccgccaga acattaatgc tgaatgagca agcgccgaca ctacaaatta
     7861 aagatgtcga tttaacacaa tatgcggaca agttaattgc acgttttgct aatccggcgc
     7921 tgaaacataa gacctggcaa atcgcaatgg atggtagcca gaaattaccg caacgtatgc
     7981 tggcaggtat tcgcatacat ctggggcgcg aaacggactg gtcgttgctg gcattaggcg
     8041 ttgctggctg gatgcgttac gtcagcggcg ttgatgatgc cggaaatgcc attgatgttc
     8101 gcgatccgct tagcgataaa attcgcgaac ttgttgcagg cagcagcagt gaacaacgcg
     8161 taaccgccct gctttccctg cgtgaagttt tcggtgatga tctgccagat aacccgcatt
     8221 ttgtgcaggc catcgaacaa gcctggcaac aaatcgtaca attcggcgca catcaggcgc
     8281 tattaaacac cctcaaaatt taacgatttc tgcggttaaa gcggatgaag ctcaccttcg
     8341 tccgctctcc ccttctcttt tctgcctttt ttagccagga ttaacgctca gttaacttac
     8401 cagagtattg cggagccgtt gtgaccagga ccaacctcat caccggtttt ctcggcagcg
     8461 ggaaaaccac gtcgattctt catctgttag cccataaaga tcccaacgaa aaatgggcgg
     8521 tactggttaa tgaatttggg gaagtcggaa ttgatggtgc tttgctcgcc gatagcggcg
     8581 cattgctgaa agagatcccc ggcggctgca tgtgctgcgt taatggttta cccatgcagg
     8641 tagggttgaa taccttactg cgtcagggaa aaccagaccg cttgttgata gagccgaccg
     8701 ggctgggcca tccgaaacag atcctcgatc tgttaaccgc accagtctat gaaccgtgga
     8761 tagatctgcg cgccaccttg tgcattctcg atccacgctt gctgctggac gaaaaaagcg
     8821 ccagcaatga aaacttccgt gaccagctgg ctgccgcaga catcattgtc gccaataaat
     8881 ccgaccatgc gacgcccgaa agtgagcaag cgctacagcg ttggtggcag caaaatggtg
     8941 gcgatcgaca attaattcac agtgagcatg ggaaagttga cggtcatctt ctggatttgc
     9001 cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca ttctcatcag catatcgtga
     9061 aaaaagggtt agcagcgtta agcctgccag agcatcaacg ctggcgtcgc agtctgaaca
     9121 gcgggcaagg atatcaggcc tgcggctgga tattcgacgc tgatacggta ttcgacacca
     9181 ttggcattct ggaatgggcg cgacttgcac cggtggaacg cgtcaaaggc gtgctgcgta
     9241 ttcccgaagg gctggtacga atcaaccgtc agggcgatga cctgcacatt gaaacgcaaa
     9301 acgttgcgcc accggacagc cgtattgagc tgatttccag cagcgaagct gactggaatg
     9361 ccttgcagag cgcgctgttg aagcttcgtt tagcgactac cgcgtaaggt tgcctgcgtt
     9421 tttcagtaag ataattagag aatatatgat taaaaatttg ccgcaaatag tgttgttgaa
     9481 tattgtcggc ctcgcgctgt ttctttcctg gtatattccc gttaatcatg gattatggtt
     9541 gccgattgat gcggatattt tttatttctt taatcagaaa ctggtcgaaa gtaaggcctt
     9601 tttatggctg gttgcattga ccaacaatcg cgctttcgac ggttgttctc tgctggcgat
     9661 gggaatgttg atgctgagtt tctggctgaa agaaaacgcc ccaggcagac gacgtatcgt
     9721 gattattggt ctggtaatgc tattaactgc agtggtatta aaccagctgg gtcaggcatt
     9781 aattcctgta aaacgggcca gcccaacatt gacgtttact aatattaacc gcgtcagcga
     9841 actgctctct gtccccacga aagatgcctc acgagatagc tttcccggcg atcacggcat
     9901 gatgctgctt attttttcgg cattcatgtg gcgttatttc ggtaaagttg caggtcttat
     9961 cgcccttatt atttttgtgt tttttgcgtt tcccagagta atgattggcg cacactggtt
    10021 tactgacatc attgtcggtt cgatgaccgt gatattgatc ggtttgccct gggtgttgct
    10081 gacgccatta agtgatcgat taatcacctt ttttgacaaa tcactaccgg gaaaaaacaa
    10141 acatttccaa aacaaataac tcacagtaat taacatcatc aggtttattt ttatagtgag
    10201 gataatcctg atgatgcgca ccgtgctttc atctatcgaa cgcaaaaatc attctctaag
    10261 taaatgaatg gattgcatgc gtttcactca atcgtacttt aattgaccaa ccccgcttat
    10321 taactttctg tatcactttt tcttataaaa aatcatgtaa aaccgctcgc caagaccgca
    10381 ccaatcgggt aatctcgaac tcgttttgcc tcggcggtag attatcctca cagcatataa
    10441 ttttgtgcgt tagtccacag atttggcctt aaggaattgt ttcaacatgc ccaggtaatt
    10501 agtctcgtgt cgcttggcat ttttttataa cgatatttgt cgttaaggac ttcaagggaa
    10561 aacaaacaac atggtcaaat ctcaaccgat tttgagatat atcttgcgcg ggattcccgc
    10621 gattgcagta gcggttctgc tttctgcatg tagtgcaaat aacaccgcaa agaatatgca
    10681 tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg caagcttctc aggatgaatt
    10741 tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt atggatcagt atgctgactg
    10801 gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa ggtatcgatt gttctggttt
    10861 cgtacagcgt acattccgtg agcaatttgg cttagaactt ccgcgttcga cttacgaaca
    10921 gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt acgggtgatt tagttctgtt
    10981 ccgtgccggt tcaacgggac gccatgtcgg tatttatatc ggcaacaatc agtttgtcca
    11041 tgcttccacc agcagtggtg ttattatttc cagcatgaat gaaccgtact ggaagaagcg
    11101 ttacaacgaa gcacgccgag ttctcagccg cagctaataa accgtttgga tgcaatccct
    11161 tggctatcct gacgagttaa ctgaaagcac tgcttaggca gtgctttttt gttttcattc
    11221 atcagagaaa atgatgtttc cgcgtcttga tccaggctat agtccggtca ttgttatctt
    11281 ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg ttcatacgcg ctcccaattc
    11341 tggacgtaag ctcctgctta cctgcattgt agcaggcgtg atgattgcga tactggttag
    11401 ctgccttcag tttttagtgg cctggcataa gcacgaagtc aaatacgaca cactgattat
    11461 cgacgtacaa aagtatctcg atacctattt tgccgacctg aaatccacta ctgaccggct
    11521 tcagccgctg accttagata cctgccagca ggctaacccc gaactgaccg ctcgcgcggc
    11581 gtttagcatg aatgtccgta cgtttgtgct ggtgaaagat aaaaaaacat tctgttcatc
    11641 tgcgactggt gagatggaca ttccactcaa tgaattgatt ccggcgctcg acattaataa
    11701 aaacgtcgat atggcgatct tacccggcac gccgatggtg ccgaacaaac ccgcaatcgt
    11761 catctggtat cgcaaccctt tgctgaaaaa tagcggtgtg tttgccgctc tgaatctcaa
    11821 cctgacgcct tctctctttt atagttcacg gcaggaagat tacgatggcg tcgccctcat
    11881 tattggcaat actgcgctat ctaccttttc ttcacgtttg atgaacgtta atgaattaac
    11941 cgacatgccg gtccgtgaaa ctaaaattgc gggcattcct ctgaccgttc ggctttatgc
    12001 ggatgactgg acatggaacg atgtgtggta cgcattttta ctgggcggca tgagtggaac
    12061 tgtcgttggc ctgctctgct attacctgat gagcgtacgt atgcgccccg gcagagaaat
    12121 catgaccgcc atcaagcgcg aacaatttta cgtggcgtat caaccggtgg tggatacaca
    12181 agctttgcga gtaacgggcc tggaagtact gctacgctgg cggcatcctg tcgcgggaga
    12241 aattcccccg gatgccttca ttaactttgc cgaatcgcaa aagatgattg tgccgctgac
    12301 tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa ttagaaaaag tgctgccagt
    12361 aggcgtcaaa tttggtatta acattgcgcc ggaccatctg cacagcgaaa gctttaaagc
    12421 agatatccag aaactgctca cttccctgcc cgcacaccat ttccagattg tgctggaaat
    12481 taccgagcgc gatatgctga aagagcaaga agccacacaa ctcttcgcct ggctgcactc
    12541 ggtcggcgta gaaattgcta ttgatgactt cggcaccggg cacagcgcgc ttatctatct
    12601 tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga tttatcaacg ccatcggtac
    12661 ggaaacgatc acttcacccg tacttgacgc ggtgctgacg ctggcgaaac gcctcaatat
    12721 gctgacggtt gctgaagggg tcgaaacgcc ggaacaggcg cgatggctaa gcgaacgcgg
    12781 cgttaatttc atgcaaggct actggattag tcgcccgtta ccgctggacg attttgttcg
    12841 ctggctgaag aaaccgtata cgccgcagtg gtaaggtgtg cttacgtccc ttattattca
    12901 tagtgaaagc atgccggatt gcggctaatg atgagtaaaa ggaaatccgt tgcagatgat
    12961 tgtgcgcata ctgctgctgt ttatcgccct gttcaccttc ggtgcgcagg cgcaggctat
    13021 caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg tacgcattta atttcaacca
    13081 ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag ataacgttgt cagccctcgg
    13141 taccttcgat aatttcaacc gctatgcact gcgcggtaac ccgggcgcac gcaccgagca
    13201 gttgtacgac acgctattta cgacttccga tgacgaacca ggcagttatt acccgctgat
    13261 tgctgaaagt gcacgctatg ctgacgatta ttcctgggtg gaggtcgcta ttaatccgcg
    13321 cgcccgtttt catgatggtt cgcccattac tgcccgcgat gtagagttta cttttcaaaa
    13381 atttatgacc gaaggcgtgc cgcaatttcg tctggtctac aacggcacca ccgtcaaagc
    13441 cattgcaccg ttaaccgtgc gcattgagtt agctaaaccc ggcaaagaag atatgctgag
    13501 tctgttttcg ctgccggtat ttccagaaaa gtactggaag gatcacaaac ttagcgaccc
    13561 gctcgccacg cctccgcttg ccagtggtcc gtaccgcgtt acgtcctgga aaatggggca
    13621 aaatattgtc tattcccgtg tgaaagatta ctgggcagca aacttaccgg taaaccgtgg
    13681 acgctggaat ttcgacacca ttcgctacga ttattacctc gatgataatg tcgcctttga
    13741 agcgtttaaa gcaggtgcct ttgatttgcg tatggaaaac gacgctaaaa actgggccac
    13801 gcgttatact ggtaaaaatt tcgataaaaa atacatcatc aaagatgagc aaaagaacga
    13861 atcagcccag gatacgcgct ggctggcgtt taatatccaa cgtccggtat tcagcgatcg
    13921 ccgggtccgg gaagcaatca ctctcgcctt tgactttgaa tggatgaaca aggcgttgtt
    13981 ttacaacgcc tggagtcgca cgaacagtta ttttcagaat accgaatacg cggccagaaa
    14041 ttaccccgac gccgcggagc tggtgcttct ggcaccaatg aaaaaagatc taccgccaga
    14101 agtcttcaca caaatctacc agccgccggt atctaaaggc gatggctacg atcgtgacaa
    14161 cctgttaaaa gccgacacac ttctcaacga agcgggctgg gtgctgaagg gtcagcaacg
    14221 cgttaatgcc acaacgggtc agccactcag ctttgaatta ttgcttcccg caagcagcaa
    14281 tagtcagtgg gtattgccgt tccagcacag cctgcaacgg ctgggtatca acatggacat
    14341 tcgcaaggtg gataactcgc aaatcaccaa ccgcatgcgc agtcgcgact atgacatgat
    14401 gccgcgccta tggcgggcga tgccgtggcc cagttccgat ttacagattt cctggtcatc
    14461 ggaatatatc aattccactt ataatgcccc cggcgtgcaa agcccggtta tcgactcact
    14521 gatcaaccaa attattgccg cgcagggaaa taaagaaaaa ttattgccgt tggggcgagc
    14581 actggatcgc gtattaacgt ggaattatta catgctgcca atgtggtaca tggcggaaga
    14641 ccgtctcgcc tggtgggata aattctccca acccgctgta cgccctgttt acagcctggg
    14701 tatcgatacc tggtggtatg acgttaataa agcggccaaa ctgccgtcag ccaggcaaca
    14761 gggagagtag atgggcgctt acctgattcg ccgtctgttg ctggtgatcc caacattatg
    14821 ggcgattatc accatcaact ttttcatcgt gcaaattgcg cctggcggtc cggtcgacca
    14881 ggccatcgcc gccattgagt ttggtaatgc cggagtatta cccggcgcag gcggtgaagg
    14941 tgttcgtgcc agccatgcgc aaacgggtgt cggcaatatc agcgacagta attaccgtgg
    15001 cggacgcgga ttagatccag aagtgatcgc tgagatcact catcgctacg gttttgataa
    15061 gccgatccac gaacgttact tcaaaatgct ctgggactac atccgctttg attttggtga
    15121 tagcctgttt cgcagcgcct cggtgctgac gctgattaaa gacagtctgc cggtttccat
    15181 caccctcgga ttgtggagca cgctgattat ctatctggtg tcgattccgt taggcattcg
    15241 caaagctgtt cataatggga gccgctttga cgtctggagt agcgcattta tcattatcgg
    15301 ctacgccatt ccggcctttt tgtttgccat cctgctgatt gtcttcttcg cgggcggcag
    15361 ctatttcgac ctgttccctc tacgcggcct ggtttccgct aactttgatt cgctgccgtg
    15421 gtatcagaaa atcaccgatt atctgtggca tatcacgctg ccggtgctgg cgacggtgat
    15481 tggtggcttt gcggcgctga ccatactgac aaaaaactca ttccttgatg aagtgcgcaa
    15541 gcaatacgtg gtgaccgcgc gtgcgaaagg ggtaagtgaa aaaaatattc tctggaaaca
    15601 tgtgttccgc aacgccatgc tgctggtgat tgccggtttt ccggcgacgt ttatcagcat
    15661 gttttttacc ggctcgctgc tgattgaggt gatgttttca ctcaatggtc tgggcttact
    15721 gggctacgaa gcgaccgtct cgcgcgatta tcctgtaatg tttggtacct tgtatatttt
    15781 caccctgatt ggcctgctgc tgaatattgt cagtgatatc agctatacgc tggttgatcc
    15841 gcgtattgat tttgagggac gttaatgtcg cgactcagcc ccgtcaatca ggcccgttgg
    15901 gcgcgttttc gtcataaccg tcgcggctac tggtcgttat ggattttcct cgtcttgttt
    15961 ggtttgagtt tgtgttctga gcttatcgcc aacgataaac cgttgctggt acgttatgac
    16021 ggcagttggt atttcccgtt gttgaaaaac tacagcgaaa gcgattttgg cggcccgctg
    16081 gcaagtcagg ctgattatca ggacccgtgg ctgaaacaac ggctggaaaa taacggctgg
    16141 gtactgtggg caccgattcg ctttggtgct accagtatca actttgctac cgataagccc
    16201 ttcccttctc caccctcccg gcaaaactgg ctgggaacgg atgccaacgg cggcgatgtg
    16261 ctggcacgca ttctctatgg cacgcggatc tcggttctgt ttggcctgat gctgactctc
    16321 tgttccagcg tgatgggcgt gctggcgggg gcgctacaag gctattacgg cggtaaagtc
    16381 gatctctggg gacaacgctt tattgaagta tggtcgggga tgccgacgct gtttttgatt
    16441 attttacttt ccagcgtcgt acagcctaac ttctggtggc tgctggcaat tactgtcttg
    16501 tttggctgga tgagtctggt cggcgtggtg cgggcggagt ttttacgtac tcgtaatttc
    16561 gactacattc gtgcggcgca ggcgcttggc gtcagcgatc gcaatatcat cctgcgtcat
    16621 atgttgccta atgccatggt cgcgaccctc acctttttac cgtttatttt atgtagttcg
    16681 atcaccaccc tgacctcact cgatttcctc ggcttcggtc tgccgctcgg ttcaccgtca
    16741 ctcggtgaac tgctgttaca agggaaaaat aaccttcagg ctccgtggct tgggatcacc
    16801 gccttcttgt cggtggcgat attattgtct ttgctgatct ttattggtga agccgtccgc
    16861 gacgcatttg atcctaataa ggcggtgtag catgacgcaa actctgttag cgattgaaga
    16921 tttgtcggtg ggttttcgcc atcagcaaac cgtacgtaca gtagtcaatg atgtttcact
    16981 acagattgag gctggcgaaa cgctggcgct ggtgggtgag tcaggttcag gcaaaagcgt
    17041 taccgcgctg tcaattttac gcctgctccc ttccccgccg gttgaatatc tctccggcga
    17101 tattcgtttt cgtggcgaat cgctgcttca tgccagcgat caaacgttgc gcggtgtacg
    17161 cggtaataag atcgccatga tttttcaaga gccgatggtg tcgttaaatc cattgcatac
    17221 cctagaaaaa cagctttatg aagtgctttc actccaccgc gggatgcgtc gggaagcggc
    17281 tcgaggcgaa attcttaact gccttgatcg cgttggtatc cgccaggcga caaaacggct
    17341 gacagattat ccgcatcagc tctccggcgg cgaacgccag cgggtgatga ttgcgatggc
    17401 gctgttaacg cgaccggaat tattaattgc cgatgaaccg accaccgcac tggacgtctc
    17461 tgtccaggcg cagattttac agctgttgcg cgaactgcaa ggcgagctga atatgggcat
    17521 gctgtttatt actcataacc tcagcattgt cagaaaactg gcccaccgcg tggcggtaat
    17581 gcaaaacggt cgctgtgtcg agcaaaatta cgccgctacg ctatttgcct cacccactca
    17641 tccttacaca caaaagctac tcaacagtga accgtcaggc gatccagtgc cgttgccaga
    17701 acctgcctca acgttgctgg atgttgaaca gcttcaggtt gccttcccca ttcgcaaagg
    17761 gattttgaag cgcattgtgg atcataatgt ggtggtgaaa aacatcagtt ttacgctacg
    17821 agcgggtgaa acactgggtt tagtgggcga gtccggttcc gggaaaagta cgacgggact
    17881 ggcgctgctg cgactgatta attctcaggg cagcatcatc tttgacggtc agccactgca
    17941 aaatttaaat cgccgccagc tgttacctat tcgtcatcgc attcaggtgg tatttcagga
    18001 tccaaactcc tcgctcaacc cacgactcaa cgttttgcag attattgagg aaggcttacg
    18061 ggttcaccag ccgacgcttt ctgccgcaca acgcgaacaa caagtgatag ccgtgatgca
    18121 tgaagtggga ttagatcctg aaacacgcca ccgttatccg gcggagttct ctggtggtca
    18181 gcgacaacgt attgcaattg ccagggcatt aattcttaag ccctcgctga tcatacttga
    18241 tgaaccgaca tcatcactcg acaaaacggt acaggcgcaa atattgacgc tattgaaatc
    18301 attgcaacaa aagcagcaac tggcctattt gtttatcagc cacgatttgc acgttgtccg
    18361 cgcgttatgt catcaggtta tcgtactgcg acaaggggaa gtagtggaac aaggaccgtg
    18421 cgcgcgcgtg tttgccgcac cgcagcagga gtatacgcgt cagctactgg cgttgagctg
    18481 acgcttaaaa aggattgtag tttgaaaagg gttcggcaat tgccacacca aaatttttca
    18541 atcggcatgt tgcagcaaac tcgtcctggc gatttacaaa caggcacggc tcaccttcac
    18601 actccagcac cgaacacgga acttcgatgt cgtttaacgc ctggcttagt ttgtacatca
    18661 ccgaccatgc attatcacca tccgggctaa gcaatttaag cgccaccagg ctgttgtcac
    18721 cgcacggtcc attttgcgga atcggttcaa ccttcgaacc tgcgaaacca gcagaccagc
    18781 gaaagttctg cggtagacga tggacgattg agagttgtaa tgaagtcact tattttcccc
    18841 ggaagcacat ttacttcaca atttgtttac atcaatttta acacatcatc aacaatccgt
    18901 ctttatacag atcgtaaaga ttgatgcctc gcctgcgtgg cctctatggc tctgatttaa
    18961 gtaatatctg cgggcgtggt cacgtttgcg gggctatatg tacccgtttc tgcgatctaa
    19021 ctcaaccttt ttaactacaa tgatgtgact ttttacataa attgatttta cataaaataa
    19081 acatatatcg ggggaatgat agatttgtgg ttgacatgca tcaacaaagg aagcctttta
    19141 gcttcctcgt tgtgcaatag atcaccgttt tttcggtcga ctggcgtaca gacagaagag
    19201 aatggagctg cttgcgcaga atgcaattga ccaaatcatc ggccatgcag agttaaaggt
    19261 cgcaagagaa agcaatgcga caacaattgc ccctatgcca aaacggaagg ttcctgccag
    19321 cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga atgaccgcca tcgcattgga
    19381 tgacaccatc gacacacagc ccacaaacgc cgcaacgcca accaccagcg atcagaatcc
    19441 cagccccagc agcgcactga tgaccatcca cgctgccata ataaattgta tccacaaccc
    19501 cgagcggaac atatttaacg cgccaatgcg gcgaacgaag cggctgttaa agatggtcat
    19561 cacgaacagg aaaacaatgt ttagtgcaaa gtaataacca aagttttctg gcgcgacgtg
    19621 gttaatttca atgtagacaa acggtccggc gctcaggaac gagaacattc cggcaaagct
    19681 gaaaccgctg gccagcatat aactcaggac acgcttatga cggaacagcg ccgcaaagtt
    19741 accaatagta gtacgaatgt gaaaaggctg gcgacgctcc ggtggtaagg tttctttgat
    19801 cagaaagaag atcattgccg aagccagaat cgccgccacg gcgaggatcc agaagatgta
    19861 atgccagctc agccacacca gcacccagcc gccaactatc ggtgccatca gcggtgcaat
    19921 ggtcgtcacc agcatgacaa acgacatcat ccgcgagaac tcttctttcg ggtagatatc
    19981 acgcatcaaa gcgttgataa ccatgctggc cgcagccgca gccagcccgt ggaaaaaacg
    20041 catcacaatc agctgatcga tggtttgtgc caacgcacac gccaccgcgg cggcggcaaa
    20101 caccagcgta ccgccgagca ccaccggctt acgcccgaag ctgtctacca tcggcccgta
    20161 gattaactgc cccaacacaa agcccagaat ataagtactg agggtcatct gcgtactgcc
    20221 cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg ggcagataca tatcaatcga
    20281 cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg acgaatgctg
    20341 tcgggtggtc acaacgggct cctgaaagtc attgaaaagt tagacgacgc tggcaatttc
    20401 ttcttcagtt aacggacgat attcaccggg ggctaaatca gcatccagcg taataccgcc
    20461 aatacgttca cgatgcagct caaccacgtg gttacccacg gcggcgaaca tgcgtttcac
    20521 ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa tcacttccag
    20581 caccgcaggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag caaattgctc
    20641 tgccgtatcg tcagctacag gtgattccag tgtcaccaga taggtcttct cgcaatgatg
    20701 gcgcggagaa gtaatgcggt gcgaccactg accatcatca gtcatcagca ccagaccggt
    20761 ggtatcgata tccagacgtc ctgcggcgtg cagtttccag gctaccggtt catccagaaa
    20821 atagagcacc gttgggtgat cagggtcgtc cgtggagcaa acatagccct gaggcttatt
    20881 gagcatgaag taacgtggac catgttgctg cgccagcggg ttgccatcgt aagcgacatc
    20941 atgttcagga agcagtttga acgctgcatt acgaacaatt tcgccatcga cggtgacacg
    21001 attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct gtgcgataaa
    21061 tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaacg gaggtcgggc attgacccga
    21121 aaatctgaac tgttgtctgc ccagtatagc ggtctaataa catccctcaa gggaaaaaga
    21181 tccatggcat actattagca gaataatcta cctacgcgag accatgattt ttacactccg
    21241 cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc ataaaacccc
    21301 tgccgttatc gtgctgccca ccggcgcagg taaaagcctg gtgattgccg aactggcacg
    21361 gctggcgcgt ggtcgtgttc tggtgctggc acacgtcaaa gaactggtgg cgcaaaacca
    21421 tgcaaagtat caggcgctgg ggctggaagc cgatattttt gccgctgggt taaagcgcaa
    21481 agagagccac ggtaaagtgg tatttggcag cgtgcagtcg gtcgctcgta atcttgatgc
    21541 ctttcagggt gaattttcgc tgttgattgt cgatgaatgc caccgtattg gtgacgatga
    21601 agagagccag tatcagcaaa tcctcactca cctgactaaa gtgaatcccc acttacgcct
    21661 gctggggctg actgccacgc cttttcgatt gggcaaaggc tggatctacc agttccatta
    21721 tcacggcatg gtacgcggcg atgagaaagc ccttttccgt gactgcattt atgagctgcc
    21781 gctgcgttat atgattaaac acggctatct gacgccgcca gaacgactgg atatgccagt
    21841 agtgcaatac gatttcagcc gcttgcaggc acagagtaac gggctgttca gcgaagctga
    21901 tctcaaccgt gagctgaaaa aacagcaacg cattacgcca catatcatca gccagatcat
    21961 ggagttcgcc gaaaagcgca aaggggttat gattttcgcc gccaccgttg aacacgcaaa
    22021 agagattgtg ggattactgc ctgccgaaga tgcagcactg attactggcg acacccccgg
    22081 cgctgagcgc gatgtgttaa ttgaagattt taaagcccag cgttttcgct atctggtcaa
    22141 cgtcgcggta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg ccattctgcg
    22201 tcctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc
    22261 tggcaagact gattgcttaa ttcttgatta tgcgggtaat cctcacgacc tctacgcgcc
    22321 ggaagttggt acaccgaaag gcaaaagtga caacgtcccg gtacaggttt tctgccctgc
    22381 ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat tgattgaaca
    22441 ctttggtcgt cgctgtcagg gatggtttga agatgacgac ggccatcgcg aacagtgtga
    22501 cttccgtttc cattttaaaa attgcccgca atgtaacgcg gaaaacgata ttgccgcccg
    22561 ccgctgccgc gaatgtgaca cggtactggt tgatccggat gatatgttaa aagcggcgct
    22621 acgactgaaa gacgcgctgg tattacgctg tagcggcatg tctttacagc atgggcacga
    22681 cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggcgcgg atgtgagtga
    22741 gcgtttccgt ctgcaaacgc ccgcccagcg aactgccttc gagcagcttt ttatccgccc
    22801 gcatacgcgc acaccgggca tccctctgcg ctggatcacc gccgccgata tcctcgccca
    22861 gcaagcctta ctgcgacatc cggattttgt cgtcgcccgc atgaaaggtc agtactggca
    22921 ggtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg aattacgcgg
    22981 ttaatggtag ttttgattga tgtataaggc gattgagtat agaatctcgc ccgcttttgc
    23041 atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt aatttaagag
    23101 agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc
    23161 gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa gcagcaccgc
    23221 tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct gaattctaca
    23281 gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct caggacgtac
    23341 agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct taattgctga
    23401 ataagttgta aaaaaccccg cttcggcggg gttttttgta tctgcagatt atgcctgatt
    23461 acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg gcattcagaa
    23521 cacaaaaccg attaattgcc gccagacgtc cggcgctgca attggtcgcg caaattcggc
    23581 ggcgtgcctt taatggtcaa ggtatcggtt gctggatccc agaagatgcg ttcacctaac
    23641 agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt cgtcaactga
    23701 cgcagtgtgc tacggtctgc cgggaagctc tcttccagtt cgtagccttt ttcagccgca
    23761 aacttcgtga agctcacttc gcttacgcct gccagctctt tcgacagcga ttccagttcg
    23821 atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg gcgcacgttc
    23881 tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc ttgcaacagc
    23941 ccacggtttt gcgctttcgc gttcaatcct tcgctggccc cgaggaaatc cataaagaaa
    24001 tccgccactt tgcgccctac tcgccctttc aggaaagtga gatagcgggt ggactctgga
    24061 ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt gatatcaaga
    24121 taatgggttg ggttgatgtc gagattttcg ttaacgcgca tactgctcag gttgctcaga
    24181 actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag cacgaatcca
    24241 ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gcccggttgc cgcgcggcta
    24301 aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc
    24361 tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg cagttctgcc
    24421 accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg
    24481 ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc cagactcatg
    24541 ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg acgcaataaa
    24601 agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca taaaaaagca
    24661 gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg atttcgattt
    24721 atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc agagctgctc
    24781 aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt aggcaacatg
    24841 gtcactaacc ttatcaacac cagcattgcc ccggcccaac gcctggcaat tgccaactct
    24901 tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg gaaacagata
    24961 acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg
    25021 ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca gccgttacct
    25081 gtttatcgcc gactggccga caacgcttgc tggtcgcatt tattcctacg taagcattat
    25141 cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc cgctgacctt
    25201 tatcgttggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa cggcgggaat
    25261 gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc ttaatcccat
    25321 cgtctggcag ttggttatca acccagacga aaatgagatg gcgcgcgact ggcagctgat
    25381 gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt ggagctggca
    25441 aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt
    25501 tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact tctatcgccc
    25561 gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc gacgtttcct
    25621 tgagaagcat ggcctgcttg atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa
    25681 tccagacgcc gtttccgtac agtatccgtt aagcgaactg cgctatcgcg atatgggcac
    25741 cgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct tcgagaagca
    25801 gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc atatgagctc
    25861 cggcaacact acagacaacg gcatctttgg cctgttctat ggcatctcgc cgagctatat
    25921 ggacggcatt ctgtcgaccc gtacccctgc ggcgttaatt actgcgctta atcagcaagg
    25981 ctatcagctg ggattattct cgtcagatgg ctttaccagt ccgctgtatc gccaggcatt
    26041 gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga ccgccacgca
    26101 gtggatcaac tggctgggcc gctacgcaca agaagataac cgctggttct cgtgggtctc
    26161 tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga aatatagccg
    26221 ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc ttgattctgg
    26281 caaactggac aatacggttg tgattatcac tgccggtcgg ggtattccgc tgagcgaaga
    26341 cgaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga ttcactggcc
    26401 aggcacgccg gcgcagcgta ttaatgcgct gactgatcat accgatctga tgacgacgct
    26461 gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag gtcaggattt
    26521 gttcaaccct caacgccgtc attactgggt cactgcagcg gataacgata cgctggcaat
    26581 taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca cttacaactt
    26641 acgtggtgaa agagtaaaag atgaaaaacc acagttaagt ttgttattgc aagtgctgac
    26701 agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcga aatatcttac
    26761 ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt agcgcagcct
    26821 ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg tgccgaccaa
    26881 aaatcccaag aaaaaaccaa cctttacggt tggttttttt atatctgcaa ttaatttgat
    26941 aaacagaccg tgacacatca cagcctgttt attttctgtt atcagaacgt ccagaccact
    27001 cccgcctgag tattccacgg cgattctacc cctgcgccat tgccatagct gactgacaaa
    27061 tgaccgctta acgtcggggt aaacgatgac cttatcccag cctgataaac gccaggggtt
    27121 ccagccacat cattattaaa tttgccatcg tcattaactt tcacatggtt agaatcggcg
    27181 tattcctgac gtacggcggc tttcagccag ggttccagcg tcgtaccatt ttgcaggtcc
    27241 atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt tcccacatca
    27301 gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc
    27361 agatagggtc taacactcca caatccgtca acccaacgga acccgctctc aacatgagca
    27421 cccgcgccgt tactattgta atcgccaaac gctgttgccc cattactcat cttgccatgg
    27481 atggtgttgg caaaacggtc aactttcacc accccatcaa cataggcacc gttctgatgc
    27541 tcccaaccgg cataagcccc cagggtatag ctatcgatat taccttttcc gccgcgatca
    27601 aaaccaatat cagaatgaga gtaaccaaag aacaagccgc gaattgtact gctttcttca
    27661 cgggagaaac ggctatcgat accgagcgtc aggcccgtca atgtttgctc aaaaccagct
    27721 cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg aactccacat cgccgtatcg
    27781 taactaacgc tttttacgct accaagacgc tcacgcacgg tgtccagttc tgcatcaaat
    27841 accagcggtt gtgcggccgc catattcagc acatcagtgg ttgaaggggt aatttgcgcg
    27901 cgattctctg ccaaactcca gctatggttg ccattatcca gcaaggtata ttcatacgta
    27961 ccgatatcaa caacgcctcc ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt
    28021 gttaccagtg taaggctatc tcctgctgcc gggctggcac cggtgtccgt cacgaatatt
    28081 ttgaaatcac ctgttgcctg accggtgacg ttgagctgat cgctctgatg attagccata
    28141 tcggtacgca tataaaaatt gccgtttccc gacagggtat tggtggtcaa cgtaatgaaa
    28201 ttttccgcac ttgccgtcac tgatgaacga gtcactggct catccatcat aatcacattt
    28261 gcgtcagaca tcgcaaggtt ggctaagttg aggagggtgt tttggttcgt cgtagtgata
    28321 tcggcaatga tggcccatac gctattttcg agcgaaacgt ccgctttgct ggtatccacg
    28381 gcaccatgcg ttctaaaaga agcgccctca gaaaccacta ccttacccac caacatagcg
    28441 ggtgcataat ttatttgcgg cttcatgacc tcaagaatgc catcattccc tctgactgac
    28501 acgttaacca ttgtgccagc ccagacatta gcgcggccat tgttgataac catattttca
    28561 gagatggctt tagaggaata cgtgatactt ccattctggt ctgatcgacc gagatcatat
    28621 accgcatttg cctgcacttt cgtaccagta tcctttccca gcgattgcat agtgacatgc
    28681 tcatcaagga tagtatcaat ggcctgcgtg tcctccataa caatgagccc ggaaccatca
    28741 tccagttcat aattttttga cacaccatct tttatactaa attggccttt actgtttgta
    28801 ccactcactt ccagcgcatt cgttgagaca attaattttc cgcctgcttt tttatcaacg
    28861 ccagttgcag tcccgccatc cataacctcc agtaagccgc cactatctac agtagtatta
    28921 taagcgaagt cattttcttc aactcgcaaa cttccgccgt tttccaacag catattgtta
    28981 gcaataccat tcttgatttc gaactgaccg agacggtttg ttccgaatgc ctccatgacc
    29041 cgcgtgcttg ctttaatagc accgcctgct ttctgatcta ccgcaatcgc taatcccccc
    29101 tctttaacct ccagtaatcc tcccttttcg atagcggcaa ggacggtaat cccatctttt
    29161 tcaacgatta agcgcccgcc tttaatcatg ggatcattcg ctgcgccata aacctgtagc
    29221 acaccattat tgagggtagt ctggtttgcc gcgccaccca tttgaacaat ttgataaccg
    29281 tcatttaata ctgagtattc tgcggtacca ttcatttcaa tgtactgata ccctccgtt
//
LOCUS       DAJTMK010000032        29323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTMK010000032 DAJTMK010000000
VERSION     DAJTMK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29323
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.033"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1748)
                     /locus_tag="OO135_001218"
     CDS             complement(<1..1748)
                     /locus_tag="OO135_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR5397141.1"
                     /translation="MLSLYEKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSR
                     FELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDD
                     NKYYLFSRRVNDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNP
                     AYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLK
                     QGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGN
                     LLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEAL
                     KIREGDAKEIISIKNSIAEMKDAEIERTNKLLSLISYDQESGFIKNMAIIESNNNQYL
                     AVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCREN
                     VQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMF
                     SEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWV
                     SAERGIISPLKFITIA"
     gene            complement(1926..2837)
                     /gene="gsiD"
                     /locus_tag="OO135_001219"
     CDS             complement(1926..2837)
                     /gene="gsiD"
                     /locus_tag="OO135_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5397142.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFGRWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPVFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(2840..3760)
                     /gene="gsiC"
                     /locus_tag="OO135_001220"
     CDS             complement(2840..3760)
                     /gene="gsiC"
                     /locus_tag="OO135_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5397143.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(3778..5316)
                     /gene="gsiB"
                     /locus_tag="OO135_001221"
     CDS             complement(3778..5316)
                     /gene="gsiB"
                     /locus_tag="OO135_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5397144.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGANFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(5336..7207)
                     /gene="gsiA"
                     /locus_tag="OO135_001222"
     CDS             complement(5336..7207)
                     /gene="gsiA"
                     /locus_tag="OO135_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5397145.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRAHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(7194..8159)
                     /gene="iaaA"
                     /locus_tag="OO135_001223"
                     /pseudo
     CDS             complement(7194..8159)
                     /gene="iaaA"
                     /locus_tag="OO135_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            8363..9598
                     /gene="moeA"
                     /locus_tag="OO135_001224"
     CDS             8363..9598
                     /gene="moeA"
                     /locus_tag="OO135_001224"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5397146.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISTGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            9598..10347
                     /gene="moeB"
                     /locus_tag="OO135_001225"
     CDS             9598..10347
                     /gene="moeB"
                     /locus_tag="OO135_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5397147.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(10423..11085)
                     /gene="fsa"
                     /locus_tag="OO135_001226"
     CDS             complement(10423..11085)
                     /gene="fsa"
                     /locus_tag="OO135_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5397148.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            11216..12115
                     /locus_tag="OO135_001227"
     CDS             11216..12115
                     /locus_tag="OO135_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5397149.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHRQKLNPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            12121..14553
                     /locus_tag="OO135_001228"
     CDS             12121..14553
                     /locus_tag="OO135_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5397150.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            14699..15514
                     /gene="ybiV"
                     /locus_tag="OO135_001229"
     CDS             14699..15514
                     /gene="ybiV"
                     /locus_tag="OO135_001229"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5397151.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            15666..16930
                     /locus_tag="OO135_001230"
                     /pseudo
     CDS             15666..16930
                     /locus_tag="OO135_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
     gene            complement(17171..18763)
                     /locus_tag="OO135_001231"
     CDS             complement(17171..18763)
                     /locus_tag="OO135_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5397152.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNDKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18982..19902
                     /gene="ldtB"
                     /locus_tag="OO135_001232"
     CDS             18982..19902
                     /gene="ldtB"
                     /locus_tag="OO135_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5397153.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19961..21079)
                     /locus_tag="OO135_001233"
     CDS             complement(19961..21079)
                     /locus_tag="OO135_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5397154.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSGPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(21076..21543)
                     /gene="mntR"
                     /locus_tag="OO135_001234"
     CDS             complement(21076..21543)
                     /gene="mntR"
                     /locus_tag="OO135_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5397155.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            21729..21857
                     /gene="mntS"
                     /locus_tag="OO135_001235"
     CDS             21729..21857
                     /gene="mntS"
                     /locus_tag="OO135_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5397156.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            22129..23712
                     /gene="opgE"
                     /locus_tag="OO135_001236"
     CDS             22129..23712
                     /gene="opgE"
                     /locus_tag="OO135_001236"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5397157.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(23761..24276)
                     /gene="ompX"
                     /locus_tag="OO135_001237"
     CDS             complement(23761..24276)
                     /gene="ompX"
                     /locus_tag="OO135_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5397158.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(24329..24394)
                     /locus_tag="OO135_001238"
     CDS             complement(24329..24394)
                     /locus_tag="OO135_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397159.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            24629..25516
                     /gene="rhtA"
                     /locus_tag="OO135_001239"
     CDS             24629..25516
                     /gene="rhtA"
                     /locus_tag="OO135_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5397160.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25816..26319
                     /gene="dps"
                     /locus_tag="OO135_001240"
                     /gene_synonym="pexB"
     CDS             25816..26319
                     /gene="dps"
                     /locus_tag="OO135_001240"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5397161.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            26723..27469
                     /gene="glnH"
                     /locus_tag="OO135_001241"
     CDS             26723..27469
                     /gene="glnH"
                     /locus_tag="OO135_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5397162.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGTLKTLRENGTYNEIYKKWFGTEPK"
     gene            27608..28267
                     /gene="glnP"
                     /locus_tag="OO135_001242"
     CDS             27608..28267
                     /gene="glnP"
                     /locus_tag="OO135_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5397163.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            28264..28986
                     /gene="glnQ"
                     /locus_tag="OO135_001243"
     CDS             28264..28986
                     /gene="glnQ"
                     /locus_tag="OO135_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5397164.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQVFLQHVS"
BASE COUNT         7272 a         7807 c         7206 g         7038 t
ORIGIN      
        1 tcagcaatgg taatgaactt cagcggtgaa atgatccccc tctccgcaga aacccaacgg
       61 cacaacgcct ccgcgcccag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg
      121 aggaatagtt cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct
      181 tctatttcgt tgagtttcgc gtcatcacag aaaataaact cggagaacat atggtcacgg
      241 atactggaaa tcgccagttt cagtttttct gcgtaactcc aggcgttttc cccctgcaaa
      301 ggttcacaaa tggcagactt atggatgcgc agattgcggt agccaaagct gctgtcaaaa
      361 tcgtttacca tcgctatttt acgggtaaat gtctgtacat tttcccgaca cagaagtaaa
      421 tagagatcgg cattgaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc
      481 tgacacaatt tcctgacgat tttattgcgt tcatcaacac caaacaccgc ttccacggct
      541 tccagaccac acagtttgat gatccccaca gccagatact gattattgtt ggactcaata
      601 atcgccatat ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta
      661 tttgtccgtt caatttcggc atctttcatt tccgcgatcg aatttttgat gctgataatc
      721 tctttcgcat cgccttcgcg aattttcaga gcctcgatct gatccgttga gtcgggcaaa
      781 aacgccagcg tattaaccag ctcggtcatt tcagagaatt gccgacggaa cacacgtgtg
      841 gtcatgaaat ataccaccag caacacgata ataatgaacg caaacggtag ataaaaaagt
      901 ttatctgatg cgttcaataa attaccgcca ggataataaa aatagatttc gaacaatgca
      961 ttatttggca ctgctgatgt ctttgcaata tactccgtat cgacagaatc aataatatta
     1021 taattacctt cgctattata aataatcagt ttttcacgag cgagttgatt cttggcaatg
     1081 atctcatgac cttgtttcaa aaatagaact tcatcattag cgctgatttg atcaagatac
     1141 tcgttcaaat accgccagtt taaatcgaca tgaattttat ctaccgctaa caaatcgtga
     1201 tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg atcaaagcaa
     1261 ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca cggtcgtttc
     1321 gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg tccatttact
     1381 tgttcctgat gataaacaac gattttatca ttcaccctcc gtgaaaagag ataatattta
     1441 ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat tttcagaatc
     1501 acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt taatgtcagg
     1561 gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag ttcaaagcgg
     1621 ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatgat gaaaaaaaga
     1681 ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt ttcgtataaa
     1741 ctcagcatgt taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata
     1801 gtcccttgct gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac
     1861 aacacttgct tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa
     1921 cgtaactatc ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg
     1981 aacgccagta cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata
     2041 accatatccg ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca
     2101 ccgaggccga gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg
     2161 aaaaacacca cgatagacga gacggtccca ggcaagatat gacgcaataa aatggtcata
     2221 tcgctggcac caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg
     2281 ttgccgcgca ccagtcgggc aaacacgggg atggaaaaaa tggcgactgc gataatcacg
     2341 ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag
     2401 gcaaacagca catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca
     2461 gccaacaagc ccagcaacgt cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc
     2521 gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg
     2581 acgccaaacc agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt
     2641 tcggcgtcat agggagcgat ccagcgtcca aaaatggcca ccacaatcaa taaaataacg
     2701 aataatgcgg cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac
     2761 ggtgtacgca cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc
     2821 cagttaaata gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca
     2881 catccaccac taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa
     2941 tcaccgggta atcgcgcatt tctacggagt caacgagtaa gcgtccaagt ccgggccagt
     3001 tgaaaacttt ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca
     3061 tggtcactac cgggatcatc gcgttacgta gcccgtgttt gaggacaacc caggtttcgc
     3121 tcaccccttt cgccctcgcg gtacgcatat aatcttcgct taaaacatcg acaaacgacg
     3181 cgcgggtaaa gcgcgccatc acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa
     3241 tgtagtgctg ccaactgtct gctcccacgg taggcagcca gcccagttca acggagaata
     3301 cctgaattaa aagcatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg
     3361 tcatactcaa tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca
     3421 taccaaatat caccgcccag accatactgg ttatggtcag ccacagcgtt ggcataaagc
     3481 ggctggcaat ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct
     3541 gcacagcatt gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct
     3601 gctgacgcac cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg
     3661 gatcgccggg cagcatatgc acaaataaaa acaccagcac cgagacgata aacagcgtcg
     3721 gaatcaaccc cagtaagcgt ttgataacgt aattaagcat tccactccct gcgttgctta
     3781 ttgcaaatcc gcgtcttcaa agctgaagcc ggtgtctggc atgatccaaa aaccggtcag
     3841 gtttttactg tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgactcttg
     3901 ccagatgata tcctgcgccg ccttatacaa gcgggtcttt tccgccggat cattagtttt
     3961 cagtgcctga gccaggaagt cgtccacctg tttattgctg taaaatgcgg tattaaacag
     4021 cgtcggtggc cagttttgcg aggcgaacag tggcgatagc gcccagtccg cttcgccggt
     4081 tgaagccgac cagccagtgt agaacatccg cacgccgctc tctttttgcc ctttaccttc
     4141 aacttctgcc gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg
     4201 cgctaactgc tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga
     4261 ccacagcgtg gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt
     4321 cactggatcg taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac
     4381 accagtagct ggcgttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat
     4441 ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat
     4501 actgatataa cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc
     4561 cagcagtgcg gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag
     4621 cattgcagcg cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg
     4681 caatcctggc tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca
     4741 ggtatccagt tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt
     4801 ttccagcgct gccggtgaaa tcatcgcggt cgccggatgg gcaagaatat taataaacgc
     4861 tgagaacggc tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc
     4921 aatattctta tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag
     4981 attcgctttc accgccgcgg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg
     5041 caatttcacg gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt
     5101 tttcagtttc atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc
     5161 ctgagataac gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg ctcctaccgc
     5221 caccaccaca tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag
     5281 cgccactaac ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc
     5341 tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt
     5401 gcaacgagac ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg
     5461 acagcagtac acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta
     5521 atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa
     5581 caatttgccc gagatacatc accgccacac gatgactaat ccgctctacc acggccatat
     5641 cgtgggagat aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga
     5701 taatctgccc gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt
     5761 ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact
     5821 catgcgggta acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa
     5881 cgcgtgcagc cgcgtcttta cctggcaata aaccgtgtgc acgcagcggt tcgataatcg
     5941 aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa
     6001 tatcccggcg caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa
     6061 agataatttc gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt
     6121 taccgctgcc agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga
     6181 ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga
     6241 aacgggtgac aagattacgc actcgtaaaa caggttcgcc atccaccacc gttttctgct
     6301 cgatgggggg ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat
     6361 aatctaaccc tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag
     6421 gatgttgcgg tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat
     6481 acatcaccag tacccgatcg gctatctctg ccaccacgcc catatcgtgc gtgataaaga
     6541 taacgcccat cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa
     6601 tagtgacatc cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca
     6661 gcgccatcgc aatcatcact cgctggcgca tcccgccaga gagttgatgc ggataacgtg
     6721 aaagaatggt ttgtgcctca ggaatgcgta cctgatccag catccgcttc gcctcgacca
     6781 tcgcttcttc acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca
     6841 cagtaaatac cgggttcagc gatgtcattg gctcctgaaa tatcatcgcc atatccgcac
     6901 cgcgaacatg gcgcatttgt gcagcgctct gctcgctaag ttcaatcact tcgcgactgc
     6961 gccgctgcaa cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca
     7021 tcaatgccaa cgcagtcact gacttaccgg agccggattc gccaacaatt gccagcgtct
     7081 caccgcgttg cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca
     7141 taaaggcaat attcagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt
     7201 gtggcaacgg tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag
     7261 ccccaggcgc gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggccg
     7321 atagcgatta agccaccgct accgacaagc gcagggagtt tttccattac tacccgctcg
     7381 caggcttccg cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc
     7441 agcgcgcgga tgaagacttc gcccgtgccg gtacaagaaa ccgccacact ggcgttattg
     7501 gcgtagcatc cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc
     7561 attccgcctg tggacgtggc tgccgccaaa ttgccgtcta aatccaactc cacggccccc
     7621 acggtgccca ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc
     7681 ccttccttgc gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatctccggc
     7741 gagacgcgct ccatgccacg agcaaacgca aaattttctg ccccttagcc aatcatcatc
     7801 acatgcgggc tttgctccat caccacccgg gcggcaagaa ccggattacg cagatgacta
     7861 acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca
     7921 tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc
     7981 agcagacgca ccgcttccgt caccacatcc agcgcacttt cgcccgcttc cagcattttc
     8041 tgcccggttt caacaatggc agacaacgcc tcaatgtagc gtaattcctg ttgcagactc
     8101 atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata
     8161 gtattcgtcc attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat
     8221 tactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg
     8281 ctacgcccga ggtggcatac agtctgtggc gtttttctga cataataggc aaattcgatt
     8341 ttgcctccgc aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc
     8401 gcttaatgag atgctttctc gcgtcacccc actgaccgcc caggaaacgc tgccactggt
     8461 acagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt
     8521 tgataactcc gcaatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc
     8581 gctgcccgtt gccggtaaat cctttgccgg tcagccgtat catggtgaat ggcctgcggg
     8641 cacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat
     8701 gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg
     8761 gcaaaatatt cgccgtcgcg gtgaagatat ctctacaggt gcggttgttt tcccggcggg
     8821 aactcgcctg actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc
     8881 ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg
     8941 tcagccgctg ggcgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt
     9001 agaacagctg ggttgcgagg taatcaactt agggattatc cgcgacgatc cccatgccct
     9061 gcgcgccgca tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt
     9121 ttcagtgggt gaagcggatt acaccaaaac gattcttgaa gagctggggg agatcgcctt
     9181 ctggaagctg gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg
     9241 gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca
     9301 gcctttgctg gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt
     9361 acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct
     9421 gcaacgcaac gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat
     9481 atttagctcc tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt
     9541 ggacgtgggc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg
     9601 gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt
     9661 gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga
     9721 ctcggctgcg cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc
     9781 gacttcgaca cggtttcgct ctcgaatctg caacgccaga cactgcacag tgatgccacg
     9841 gtcgggcaac cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc
     9901 gcgattacgc cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa
     9961 cacgatctgg tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc
    10021 tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc
    10081 acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc
    10141 gagaacgcgt taacctgcgt agaagcaggc gtgatggcac cgttgatcgg cgtaattggt
    10201 tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg
    10261 aaaatcgtca tgtatgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat
    10321 ccggggtgtg aggtgtgcgg gcagtaattg ccacctccag gccagataag acgcggcact
    10381 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
    10441 aaacgctccc agccagtcct gttcaaactt cgccacagcg gcatcaaccg ccggatagct
    10501 aatcatctgt tgtgccacat ccagtggcag cgtaattgat tcacatcctg ccagtaagca
    10561 gtccagcgcc tgacgcgggt ttttgaaact cgctgccagc actttcgcct gcggcgcatg
    10621 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
    10681 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcagcccttg
    10741 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
    10801 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
    10861 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
    10921 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
    10981 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
    11041 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    11101 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    11161 aatccaactt tcattcaaaa gtgttttaat gtttgaacga aatagagagg caactatgat
    11221 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    11281 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    11341 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc
    11401 gccggaagtg attgagcgcg cattgaatgg tttgcttatt catcggcaaa agttaaaccc
    11461 ggagcatctg acggcgctaa ccgactgctg cccgacacag gcgttaaccg tgtgtgggga
    11521 agtgaaaagc gttgaggaga tcatggcgac cgttctgcgc gataaaccgt tttacgatcg
    11581 cagcggcggc ggcttaacgc tttcgggtgg tgaacccttt atgcagccgg aaatagcgac
    11641 ggcgctactg caagcctgcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    11701 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    11761 aaaacacatt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct
    11821 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    11881 tcagggcttt aatgccgacg aaacctctgt aaaagccatt accgattttg ccgccgacga
    11941 gctgcacgtt ggcgaaattc attttctgcc ctaccacacg ctgggcatca acaaatatca
    12001 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag acctgctcga
    12061 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    12121 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    12181 gtgcatattg tgaaaccgcc ggtctgtacc gagcgcgcgc agcactatac cgagatgtat
    12241 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggctctggc gcatcacctg
    12301 gcgaatcgca ccatctggat caagcacgat gagttgatca tcggcaacca ggcaagcgag
    12361 gttcgcgctg cgccgatctt cccggaatat accgtctcgt ggatcgaaaa agagattgat
    12421 gatctggcag atcgtcccgg tgctggtttt gcggtgagcg aagagaacaa acgcgttctg
    12481 catgaagtgt gcccgtggtg gcgcggtcag acggtacagg atcgctgcta cggcatgttt
    12541 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    12601 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    12661 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    12721 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    12781 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    12841 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    12901 ttctggcagg cgctgcaact gtgttacttc atccagttga tattgcagat cgaatctaac
    12961 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    13021 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    13081 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    13141 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    13201 gacgcggtga atccactctc ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact
    13261 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    13321 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    13381 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    13441 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    13501 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaac
    13561 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    13621 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    13681 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    13741 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    13801 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    13861 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    13921 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    13981 gacgatgata ctgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    14041 aaacagtacc ataatccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    14101 acgtcatcta tctccgctaa cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg
    14161 cgtaaagccc acaccccgct ggcagaaggc gctagtccgg cctctggtac tgaccatctc
    14221 ggccctactg cggtcattgg ctcggtgggt aaactgccta cggcagcgat tctcggcggc
    14281 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaag
    14341 ctgatgatcc tgctgcgcac cttcttcgag gtgcataaag gctggcatat tcagtacaac
    14401 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    14461 gtagtgcgtg tcgcgggtta ttccgccttc ttcaccgcgc tctctccaga cgctcaggac
    14521 gatatcatcg ctcgtactga acatatgctg taatctcttc ggcccggctg cggcgtcgcc
    14581 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    14641 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    14701 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    14761 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt
    14821 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    14881 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    14941 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    15001 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    15061 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    15121 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    15181 gatcgacaaa ctgcacatag cgttcgatgg cattatgaag cccgtcacca gtggttttgg
    15241 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    15301 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    15361 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    15421 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    15481 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    15541 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    15601 tttataactt atattgtcac atgcaaatca taatggtttc gaatgaaagt taatgagagg
    15661 tggttatggc ttctactttt accagcgaca cattgccagc cgatcacaaa gcagctatcc
    15721 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    15781 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggtgatg
    15841 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    15901 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    15961 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    16021 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    16081 ccatctactg gttgcagacg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    16141 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    16201 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    16261 gtctggagcg cataccgact ccggggcgct ggaacgctgg ctgcttctag cgtatcagcg
    16321 cgttttcgcc aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca
    16381 tcgtacggaa gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac
    16441 attccagggc tggacagcgc tctctgatat gctgcctggt caggggttgc tgcacgttgt
    16501 gcccattcct gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga
    16561 ggatgaactg tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc
    16621 actgttaatt gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg
    16681 gcactgcgac gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt
    16741 gatgtacatt cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa
    16801 ggccgcactg gaaaaaggcg catcgccggg cgacttcccg cgcgaggact atgaaacaaa
    16861 ctgggaagga cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggcat
    16921 ggatgtttga gagttaatgg aagtgaattg atgtgggatt aagaaaatcg attcgacgtt
    16981 actcagattg ctgacaagtg cgctttctta atgcctgatg cggcgttaac gccttatctg
    17041 gcctacaaag tcctgcagat tcaatatatt acaggtactg cgtaggcctg ataagcgtag
    17101 cgcatcaggc tattttgcat ttgtcatcag ccgccgtcac cccgacagta tcggggtgac
    17161 ggataatcac ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt
    17221 cgatcacgcg ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac
    17281 ggtcgtgtga aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact
    17341 cgatggattc catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct
    17401 gcatcattaa cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg
    17461 gctttttgat gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct
    17521 cgtcatcgcc ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa
    17581 actcatattc gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg
    17641 tgccgctgtc cggttgcaga tcgcccacca gcgttttcat cagcgttgat ttaccgacgc
    17701 cgttggtacc cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa
    17761 acagtggacc gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca
    17821 gtttcttatc ctgttcaaaa cggatgaacg ggttctgacg gctggaggct ttcacctctt
    17881 ccagtttgat tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt
    17941 tggcgctaaa gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttgtcgt
    18001 tatcggccag cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac
    18061 ccggataaac gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa
    18121 ggaagtgacg gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt
    18181 ccagccagcg aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat
    18241 ccggatcagc aaacagcgcc tgcgccagaa gcacacgcag cttccagcca ggagcaactt
    18301 cactcatcgg gccgtagtgc tgttccactg gaattcccac gccaagcaac aattcaccgg
    18361 cgcgagcttc cgcagagtaa ccgtccattt cgccgtattt aacttccaga tcggccactt
    18421 tatagccgtc ttcctcactc atttccggca aagcatagat gcggtcacgc tcctgcttca
    18481 cttcccacaa ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg
    18541 caaactgatc ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca
    18601 aagtcggctc taagtcgccg ccgaggatct tcataaaggt ggatttacca ctaccgttcg
    18661 cgccaatcag gccgtaacgg ttgccgccgc caaatttgac ggaaatgttt tcaaacaacg
    18721 gcttactgcc gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag
    18781 agatatgaca aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa
    18841 ggtctacact caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga
    18901 gctggaatga acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc
    18961 aggcacaaaa tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc
    19021 gctgttgtcg gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg
    19081 agtcgcctgg ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg
    19141 gagtattttg ccgcggagta ccagatgggg ctttccaata tgatggaagc gaacccgggt
    19201 gtggatacct tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg
    19261 ccggataccg ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat
    19321 ccgaaaggga ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat
    19381 acgcctatca actggaccac caaagttgag cgtaagaaag caggcccgac ctggacgccg
    19441 accgccaaaa tgcacgcaga gtaccgcgct gcgggcgaac cgcttccggc tgtcgttccg
    19501 gcaggtccgg ataacccgat gggtctgtat gcactctaca tcggtcgcct gtatgctatc
    19561 catggcacca acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg
    19621 cgtaacgaag acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt
    19681 attgatgagc cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat
    19741 aacccgctgt ctaccaccga agcccagttt gaaggtcagg aaattgtgcc aattaccctg
    19801 acgaagagcg tgcagacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa
    19861 gcgattaaaa accgctccgg gatgccggtt cgtctgaatt aatcttcaaa acttaaagca
    19921 aaaggcggac tcataatccg ccttttttat ttgccagacc ctagttggcc gggagtataa
    19981 ctaacaaaac atatcccacc aacgccgccc acaacagcat cggtattgaa tagagatgga
    20041 aacgccacca gatgcggcga tcgttcgcca tacgtagcgc aatcaaactt gccagcgatc
    20101 cgggtaataa cccaaagcca cctacgttta ccgcccatgc cagtaataaa gacggcggca
    20161 catagttcag caacaatatg gtactcggca cgttactgat cacctgcgat aaaccgattg
    20221 ccgttaacca taacccgggt tcagatagat gactcacgtt acccaacacg ccttgcaacg
    20281 ctggaagctg ggtcagtaaa tggacgtcga taaacatcgc cataaacacc agcagcagcg
    20341 tccagtccac actgagcacc acgcgacgtg ccagcagcgc aaagcctgcc gccacaatca
    20401 ccagtcccca cagctcttgt ttgaactcca gcgccgttag aaagacgata tacagcccca
    20461 gacaactcca caccagccgc ggtttccact ccggtgtttg cacccccgta tggtattgca
    20521 gcacctttcc agggaaacaa caccagcaca ggagcaggag cgtcagcatc attgcgccag
    20581 ccagcggtgc catttgggca ataaatccgg caaacgaaag accagaacgt ccccagataa
    20641 gaatattttg cgggttgcca attggcgtca gtagcgagcc agcattgact gccagcgcct
    20701 caaaaataat cagccgatta accgggatct cacacagtct ttttagcgtg atagtcagcg
    20761 gaacaacaat aaacagcgcg acatcgttgg tcagaaaggt agaaagcagc gccgccgcca
    20821 gcaccataaa catcgccagc cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca
    20881 gcacatcaaa ataaccgctt aactccacac ctttggtcag cagcatcagg ccgcttaagg
    20941 tgatgatggt gtgccagtcg atagcagcag gcccggattt cggtgcaaag ggcacgaaaa
    21001 agcttaatcc gataccaaca agaattaata actgaaaaaa acgatcgcct tgcagcgtgc
    21061 gtaaaaaagg caggctcatt tggcaccgtg tttctgggta aacaaacgaa aagcgtcgag
    21121 cgtctcttca ctaacatggt gctccatgcc ttccgcgtcg cgacgggcga tttccggact
    21181 gacgcccaac accaacaaga aattttcgac tatctgatgt cgctcgcggc tttcctgcgc
    21241 cagcttctct ccttctgccg ttaaaaacac gccacgccag gggatcattt caatcagccc
    21301 catggttgcc agccgcttaa gcattttagc caccgtcggt tgcgaaactc ccagacgagc
    21361 agccatgtcc acctgacgag cttcccccac ttccctgatc aagtcagaaa tcagctcaac
    21421 gtaatcatca ataagctcgc gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg
    21481 ctcttccacg ttcactaact gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact
    21541 cattgtgctt cctcattatg gtgacgcata catagcgttc tcatttcgtg cgcacattgt
    21601 aaaccagagt tgcgaaggta caaaaaatta acgttttagc aatagctata taatatagcc
    21661 tgtgctatat ctgtatgtag tgcaatcatc cctcaaggat cgacgggatt aacaagtcag
    21721 gaggtcttat gaatgagttc aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag
    21781 tacggttaat actgctctct atgttgtgcg atatggtcaa caacaaaccg cagcaagata
    21841 aaccttccga taaatagcgg cgtcgcggta cgccgcttca ctcctgcttt catgcaggca
    21901 taacgcgttt tggtctgaaa aaccccactt tttgtcggat ttgcaatccc cttcgcaaaa
    21961 gatttgttcg tcagtagttg acctgaacgg cggctcgctc tatcttcttg cagccctgcg
    22021 tatattgcgg ctcgcggatg cggacccctt tccactcttc acgcactctt gcaggtattg
    22081 acccttgacg ccagggtaag cacatggcgt ttgttacgat agtggcgtat gaatttaacc
    22141 ctcaaagaat cgcttgttac ccgtagccgg gtatttagcc cgtggactgc gttctacttt
    22201 ttacagtcgc tattaattaa cctcggctta ggttacccct tcagtttgct ctacaccgct
    22261 gcgtttacgg ctattttgct tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg
    22321 gtcggtgtca gttcgctggt ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg
    22381 cctaatttca atacattgct ggcattgcac tccaccaata tggaagagtc gaccgaaatc
    22441 ctgacgattt ttccgtggta cagctacctg gtcggcttat ttatttttgc gctcggcgta
    22501 atagcaatca ggcgaaaaaa agagaatgaa aaagcgcgct ggaatacctt cgacagcctg
    22561 tgtctggtat tcagtgtggc gacatttttt gttgctcccg tgcaaaacct ggcctggggt
    22621 ggcgtattta aactgaaaga tactggctat ccggtatttc gttttgctaa ggatgtcatc
    22681 gtcaataata acgaggtgat tgaagagcaa gaacggatgg caaaactttc cggaatgaaa
    22741 gatacctgga cggtcactgc cgttaagccg aagtatcaga cctatgtggt ggtgatcggt
    22801 gaaagcgcgc gtcgcgatgc cctcggtgcc tttggcggtc actgggacaa taccccgttt
    22861 gccagcagcg ttaacggttt gatatttgct gactacattg ccgccagtgg ctccacgcag
    22921 aaatcgcttg gcttaacgct caatcgcgta gtcgatggca aaccacagtt tcaggataac
    22981 tttgtcaccc tggcaaatcg cgcgggcttc cagacctggt ggttttccaa ccagggccaa
    23041 atcggcgaat acgatactgc tatcgccagc atcgccaaac gtgcagatga agtgtacttc
    23101 ctgaaagaag gtaattttga agcagataaa aacacgaaag acgaagcgtt actggatatg
    23161 accgctcaag tgctggcgca agagcactcg caaccgcagc tgattgttct gcatctgatg
    23221 ggctcgcatc cgcaggcctg cgacaggaca caaggcaaat acgaaacctt tgtgcaatcg
    23281 aaagaaacgt cgtgctatct ctataccatg acgcaaacgg acgatttact gcgcaagctg
    23341 tacgatcagt tacgcaacag cggcagcagc ttctctctgg tttacttttc tgaccacggt
    23401 ctggccttta aagagcgcgg caaagacgtg caataccttg cccatgatga taaatatcag
    23461 caaaatttcc aggtgccttt tatggtcatt tccagcgacg ataaagcgca tcgggtgatt
    23521 aaagcccgcc gctcagccaa tgacttctta ggctttttct cgcagtggac gggaattaaa
    23581 gcgaaggaaa taaatatcaa atacccgttt atatctgaga agaaagccgg gccgatatac
    23641 atcaccaact tccagttaca gaaggtggat tacaaccatc tcggaaccga tattttcgac
    23701 ccgaaacctt aaaaacaaaa atccgccccg agaggcggat tttttatatc accaaagtga
    23761 ttagaagcgg taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct
    23821 ctgctcgtaa gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc
    23881 gtaggagaaa ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa
    23941 tttaccataa cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc
    24001 cggaccagca gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg
    24061 acttttctcg gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata
    24121 gcggtatttc aggttgaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc
    24181 gtaaccgcca gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac
    24241 tgcggccagt gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat
    24301 aagtcctaag ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc
    24361 taacgggcca tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat
    24421 cactttttca gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta
    24481 ttcctaatca ccagactaat gattccatca atcctggcgc attttagtca aaacggggga
    24541 aaattttttc aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac
    24601 ttaagtctag atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg
    24661 gttaccaata gtcatattgc tcgttgccat ggcgtctatt cagggtggag cctcgttagc
    24721 taagtcactt tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg
    24781 aacgctgatc ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg
    24841 gttaccgctg ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc
    24901 tattcagaca gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt
    24961 ggcgctgttc tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg
    25021 tctgtggttc ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc
    25081 gctggcactg ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg
    25141 agcggaacat ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt
    25201 gccaattgga gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg
    25261 tctggctgtc gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac
    25321 ccgtttgcca acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt
    25381 ttccgggatg attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc
    25441 tatcatcgcc gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga
    25501 attagacatt aattaaattt acatttctgc atggttatgc ataaccatgc agaatttctc
    25561 gctacttttc ctctacaccg tctttaatat atcgaattat gcaaaagcat atttattccg
    25621 aaaattcctg gcgagcagat aaataagaat tgttcttatc aatatatcta actcattgaa
    25681 tctttattag ttttgttttt cacgcttgtt accactatta gtgtgatagg aacagccaga
    25741 atagcggaac acatagccgg tgctatactt agtctcgtta attactggga cataacatca
    25801 agaggatatg aaattatgag taccgctaaa ttagttaaat caaaagcgac caatctgctt
    25861 tatacccgca acgatgtctc cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc
    25921 caggttatcc agtttattga tctttctttg attaccaaac aagcgcactg gaacatgcgc
    25981 ggcgctaact tcattgccgt acatgaaatg ctggatggct tccgcaccgc actgatcgat
    26041 catctggata ccatggcaga acgtgcagtg cagctgggcg gtgtagctct ggggaccact
    26101 caagttatca acagcaaaac cccgctgaaa agttacccgc tggacatcca caacgttcag
    26161 gatcacctga aagaactggc tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg
    26221 attggcgaag cgaaagatga cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg
    26281 gataaattcc tgtggtttat cgagtctaac atcgaataaa tccatcgctg atggtgcaga
    26341 actttagtac ccgataaaag cggcttcctg acaggaggcc gttttgtttt gcagcccacc
    26401 tcaacgcact tatttagtgc atccatctgc tatctccagc tgattaagta aattttttgt
    26461 atccacatca tcacacaatc gttacataaa gattgttttt tcatcaggtt ttacgctaaa
    26521 taatcactgt gttgagtgca caattttagc gcaccagatt ggtgccccag aatggtgcat
    26581 cttcagggta ttgccctata aatcgtgcat cacgtttttg ccgcatctct aaaaatcaat
    26641 gagttgcaaa actggcacga ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg
    26701 tggatagaaa aaaggaaatg ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac
    26761 cctggttttt gcggtttctt ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac
    26821 cgccttcgtt ccgtttgaat ttaaacaggg cgataaatat gtgggctttg acgttgatct
    26881 gtgggctgcc atcgctaaag agctgaagct ggattacgaa ctgaagccga tggatttcag
    26941 tgggatcatt ccggcactgc aaaccaaaaa cgtcgatctg gctctggcgg gcattaccat
    27001 caccgacgag cgtaaaaaag cgatcgattt ctctgacggc tactacaaaa gcggcctgtt
    27061 agtgatggtg aaagctaaca ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt
    27121 ggttgctgtg aagagcggta ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa
    27181 agatctgcgt cagttcccga acatcgataa cgcctatatg gaactgggca ccaaccgcgc
    27241 agacgccgtt ctgcacgata cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg
    27301 tcagttcaaa gcggtaggtg actctctgga agcgcagcaa tacggtattg cgttcccgaa
    27361 aggtagcgac gagctgcgtg acaaagtcaa cggcacgttg aaaaccctgc gcgagaacgg
    27421 aacttacaac gaaatctaca aaaaatggtt cggtactgaa ccgaaataat aacgctacac
    27481 ctgtaaagcg cactggcagt tccctctccc ctctggggag aggattaggg tgaggggcgc
    27541 aaacccgctc cggggccatt aattaccctg aatttgatta tttacaccac ggtaacagga
    27601 acaacatatg cagtttgact ggagtgccat ctggcctgcc attccgcttc tgattgaagg
    27661 tgccaaaatg accctgtgga tttcggtcct cggtctggct ggcggtctgg taatcggatt
    27721 gctggcaggt tttgcacgca ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt
    27781 tattgaagtg atccgcggca cacctatcgt cgtccaggtg atgtttatct atttcgccct
    27841 gccgatggcg tttaacgact tacgcatcga cccatttact gcggcggtgg tcaccatcat
    27901 gatcaactcc ggcgcgtaca ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa
    27961 aggttttcgt gaagcaggac tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt
    28021 cattttaccg ctggcactgc gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag
    28081 cattaaagac acctcgctgt ttattgttat cggcgtggcg gaactgaccc gtcaggggca
    28141 agaaattatt gccggtaact tccgcgccct tgagatctgg agcgccgtgg cggtgttcta
    28201 tctgattatt accctggtgc tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat
    28261 cctgtgattg aatttaaaaa cgtctccaag cactttggcc caacccaggt gctgcacaat
    28321 atcgatttga acattgccca gggcgaagtc gtggtgatta tcgggccgtc cggttccggt
    28381 aaatcgaccc tgctgcgctg catcaacaaa ctggaagaaa tcacctccgg cgatctgatt
    28441 gtcgatggcc tgaaggttaa cgatccgaaa gttgacgagc gcctgattcg ccaggaagca
    28501 ggtatggtgt tccagcagtt ttacctcttc ccgcatctga cggcgctgga aaacgtcatg
    28561 tttggtccgc tacgcgtgcg tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag
    28621 ctgctggcga aagtcggtct ggcagaacgt gcacatcact acccttccga actttctggt
    28681 ggtcaacagc agcgtgtggc gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg
    28741 tttgatgaac cgacttccgc tcttgacccg gaactgcgcc atgaagtgct gaaggttatg
    28801 caggatctgg ctgaagaagg gatgacgatg gtgatcgtga cccacgaaat cggttttgcc
    28861 gagaaagtag cttcgcggct gatctttatc gacaaaggcc ggattgcgga agatggcaat
    28921 ccgcaggtgt tgatcaagaa cccgccgagc cagcgcttgc aggtattttt gcagcacgtc
    28981 tcttaattag acacattgcc tgatgcgcta agcttatcag gcctacagga tatctggcaa
    29041 cttattaaaa ttgcatgaac ttgtaggacg gataaggcgt tcacgccgca tccggcaaaa
    29101 aagcccgcac gttgtcagca atctgcttaa tatcccttcc tccctttcac ccgaaaggga
    29161 ggcacaccag attcctctca tttaaaatcg ccccgttact ttattcgtac cccttataat
    29221 ggggtgttag ccagccagac ccggcatgat tactgccccc agtcgtccat aatccggggg
    29281 gtgatgtcac cgggtctggt ggggcgctgg taaccgctaa tag
//
LOCUS       DAJTMK010000033        29267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTMK010000033 DAJTMK010000000
VERSION     DAJTMK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29267
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.034"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..156
                     /locus_tag="OO135_001244"
     CDS             <1..156
                     /locus_tag="OO135_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCR5397165.1"
                     /translation="INYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELT
                     LVKSFDW"
     gene            431..3280
                     /gene="rcsC"
                     /locus_tag="OO135_001245"
     CDS             431..3280
                     /gene="rcsC"
                     /locus_tag="OO135_001245"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5397166.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSVAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRA
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPSEFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVLNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3480..4130)
                     /gene="rcsB"
                     /locus_tag="OO135_001246"
     CDS             complement(3480..4130)
                     /gene="rcsB"
                     /locus_tag="OO135_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5397167.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4147..6819)
                     /gene="rcsD"
                     /locus_tag="OO135_001247"
     CDS             complement(4147..6819)
                     /gene="rcsD"
                     /locus_tag="OO135_001247"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5397168.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDARESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGLGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7558..8688
                     /gene="ompC"
                     /locus_tag="OO135_001248"
     CDS             7558..8688
                     /gene="ompC"
                     /locus_tag="OO135_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5397169.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINGRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8800..9855
                     /gene="apbE"
                     /locus_tag="OO135_001249"
     CDS             8800..9855
                     /gene="apbE"
                     /locus_tag="OO135_001249"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5397170.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSVELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9929..10993
                     /gene="ada"
                     /locus_tag="OO135_001250"
     CDS             9929..10993
                     /gene="ada"
                     /locus_tag="OO135_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5397171.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            10993..11643
                     /gene="alkB"
                     /locus_tag="OO135_001251"
     CDS             10993..11643
                     /gene="alkB"
                     /locus_tag="OO135_001251"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5397172.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPNLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDMVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            11719..13362
                     /gene="yojI"
                     /locus_tag="OO135_001252"
     CDS             11719..13362
                     /gene="yojI"
                     /locus_tag="OO135_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5397173.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13580..15226
                     /gene="mqo"
                     /locus_tag="OO135_001253"
     CDS             13580..15226
                     /gene="mqo"
                     /locus_tag="OO135_001253"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR5397174.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15376..15864)
                     /gene="eco"
                     /locus_tag="OO135_001254"
     CDS             complement(15376..15864)
                     /gene="eco"
                     /locus_tag="OO135_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5397175.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESPLKVELLIGQTLEVDCNLHRLGGKLESKTLEGRGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15999..16163)
                     /locus_tag="OO135_001255"
     CDS             complement(15999..16163)
                     /locus_tag="OO135_001255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397176.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16272..16766
                     /gene="napF"
                     /locus_tag="OO135_001256"
     CDS             16272..16766
                     /gene="napF"
                     /locus_tag="OO135_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5397177.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16756..17019
                     /gene="napD"
                     /locus_tag="OO135_001257"
     CDS             16756..17019
                     /gene="napD"
                     /locus_tag="OO135_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5397178.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17016..19502
                     /gene="napA"
                     /locus_tag="OO135_001258"
     CDS             17016..19502
                     /gene="napA"
                     /locus_tag="OO135_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5397179.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            19509..20204
                     /gene="napG"
                     /locus_tag="OO135_001259"
     CDS             19509..20204
                     /gene="napG"
                     /locus_tag="OO135_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5397180.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20191..21054
                     /gene="napH"
                     /locus_tag="OO135_001260"
     CDS             20191..21054
                     /gene="napH"
                     /locus_tag="OO135_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5397181.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21051..21500
                     /gene="napB"
                     /locus_tag="OO135_001261"
     CDS             21051..21500
                     /gene="napB"
                     /locus_tag="OO135_001261"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5397182.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21510..22112
                     /gene="napC"
                     /locus_tag="OO135_001262"
     CDS             21510..22112
                     /gene="napC"
                     /locus_tag="OO135_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5397183.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCRDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22131..22748
                     /gene="ccmA"
                     /locus_tag="OO135_001263"
     CDS             22131..22748
                     /gene="ccmA"
                     /locus_tag="OO135_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5397184.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22745..23407
                     /gene="ccmB"
                     /locus_tag="OO135_001264"
     CDS             22745..23407
                     /gene="ccmB"
                     /locus_tag="OO135_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5397185.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23449..24186
                     /gene="ccmC"
                     /locus_tag="OO135_001265"
     CDS             23449..24186
                     /gene="ccmC"
                     /locus_tag="OO135_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5397186.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24183..24392
                     /gene="ccmD"
                     /locus_tag="OO135_001266"
     CDS             24183..24392
                     /gene="ccmD"
                     /locus_tag="OO135_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5397187.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24389..24868
                     /gene="ccmE"
                     /locus_tag="OO135_001267"
     CDS             24389..24868
                     /gene="ccmE"
                     /locus_tag="OO135_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5397188.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24865..26808
                     /gene="ccmF"
                     /locus_tag="OO135_001268"
     CDS             24865..26808
                     /gene="ccmF"
                     /locus_tag="OO135_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5397189.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLLNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVVTIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            26805..27362
                     /gene="dsbE"
                     /locus_tag="OO135_001269"
     CDS             26805..27362
                     /gene="dsbE"
                     /locus_tag="OO135_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5397190.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27359..28411
                     /gene="ccmH"
                     /locus_tag="OO135_001270"
     CDS             27359..28411
                     /gene="ccmH"
                     /locus_tag="OO135_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5397191.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28446..29093)
                     /gene="narP"
                     /locus_tag="OO135_001271"
     CDS             complement(28446..29093)
                     /gene="narP"
                     /locus_tag="OO135_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5397192.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6690 a         7225 c         8105 g         7247 t
ORIGIN      
        1 atcaactact atggtgggcg cagcgaaacg gaattaacca ccgcccaact gacgctgatc
       61 accgatgaag gatcggtcaa tgagaaacag gaaacattta ttgtaccgat gcgtaatgct
      121 ggcgagctga cgctggtgaa aagttttgac tggtgatcca tcccttcaca caacctggct
      181 ctctcttttt tcagataaga ctgctgggcg atctgccgac gccaggatat aaccattaac
      241 aagcgtcggc agattggtca tttcttattt ttcgtactga gaactgtcca tctactccgg
      301 ctttcctggt taaggcaatg gtttctaagc gatacgcatt attgcaataa tgacagaaca
      361 ttgcggtaac acgcttttac cgctacctta accacactcc atcggtcacc tgaggcggag
      421 cttcgcccct ttgaaatacc ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat
      481 gttcagagca ttggcgttag tgctctggct gttgattgct ttttcatccg ttttttacat
      541 cgttaatgcg ttacatcagc gagaatcgga aattcgtcag gaatttaatc tgagttccga
      601 tcaggctcag cgctttattc aacgcacctc tgatgtgatg aaagagctga agtacatcgc
      661 cgaaaatcgc ttatcggcag aaaacggtgt gctttccccg cgtggacgag aaacgcaggc
      721 ggatgtgcct gcgtttgaac cgctgtttgc cgactccgat tgttccgcaa tgagtaacac
      781 ctggcgaggt tctctggagt cattggcgtg gtttatgcgc tactggcgcg ataatttttc
      841 tgtggcttac gatctaaacc gggtattttt aatcggcagc gataacctct gcatggccaa
      901 tttcggtctg cgtgatatgc cagtggaacg cgataccgcg ttgaaagctt tgcatgaacg
      961 catcaataaa tatcgaaatg caccacaaga tgatagcggc agtaacctct actggatcag
     1021 cgaaggtccg cgccctggcg tcgggtattt ttacgcgttg acgccagttt atctggcgaa
     1081 ccggttgcag gcgcttttgg ggggcgagca gaccatccgg atggagaact ttttcttacc
     1141 gggtacgttg ccgatggggg ttaccattct tgatgaaaat ggtcataccc tgatttcgct
     1201 taccggacca gaaagtaaaa ttaagggcga tcctcgctgg atgcaggaac gctcctggtt
     1261 tggctatacg gaagggttcc gggagctggt gctgaagaaa aatctgccac cctcatcgct
     1321 aagcatcgtg tattcggtgc cggttgataa ggtgctggaa cgcattcgca tgttgatcct
     1381 taacgcaatt ttgctgaatg tgcttgccgg agctgcattg tttactctcg cacggatgta
     1441 cgagcgacgt attttcattc cggcggaaag cgacgccctg cgactggaag aacatgagca
     1501 gttcaatcgc aagattgtcg cctccgcgcc agtgggtatc tgcattttgc gtaccgctga
     1561 tggcgtcaat attttaagta acgaactggc gcatacctat ctcaatatgc ttacgcatga
     1621 ggaccgccaa cgactgacgc aaattatctg tgggcagcag gtcaattttg ttgatgtcct
     1681 gaccagcaac aataccaatc tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa
     1741 cgtggccatt tgtgtgctgg tggatgtttc ttcgcgcgcg aagatggaag agtcgttgca
     1801 ggagatggca caagcagcgg aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt
     1861 cagtcatgag ctgcgaacgc cgctgtatgg cattatcggt aacctggatc tgttgcaaac
     1921 caaagagtta ccgaaaggcg tcgatcggct ggtgacggca atgaacaact cttccagcct
     1981 gttgttgaaa attatcagcg atattctcga tttctcgaag attgaatcgg aacagttgaa
     2041 gatcgaaccg agtgagtttt caccgcgtga agtgatgaac cacatcaccg ccaactattt
     2101 accgctggtg gtacgcaagc agttaggctt gtactgcttt attgaaccgg atgtgccagt
     2161 ggccttaaat ggcgacccga tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc
     2221 cataaaattc accgataccg gctgtatagt tttgcatgtc cgcgctgatg gcgattatct
     2281 ctctatccgt gttcgcgata ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt
     2341 tgatcccttc ttccaggtcg gaacgggcgt acagcgaaac ttccagggga ccggtctggg
     2401 tctggcgatt tgtgaaaaac tgatcagcat gatggacggc gatatctcgg tagattcaga
     2461 accgggaatg ggcagccagt ttaccgtgcg tattccgttg tacggcgctc agtacccgca
     2521 gaaaaaaggc gtggaagggt tgagtggtaa acgctgctgg ctggcggtcc tcaatgcgtc
     2581 gctctgtcag ttcctggaaa ctagtttgca gcgcagcggc atcgtcgtta caacatacga
     2641 agggcaggaa ccgactcccg aagatgtgtt aattactgac gaggtagtga gtaaaaaatg
     2701 gcagggtaga gcggtagtga ccttctgtcg tcgccatatt ggtattccgc tggagaaagc
     2761 gccaggggag tgggtacaca gtgtggctgc cccgcatgag ctaccggcat tgttggcgcg
     2821 tatttatttg atcgagatgg agagcgacga tcctgctaac gctctgccgt cgacggacaa
     2881 agcggtcagc gataatgacg atatgatgat tctggtcgtg gatgatcatc cgattaaccg
     2941 acgtctgctg gcagatcagt tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg
     3001 tgtcgatgcg cttaatgtac ttagcaagaa tcatattgat atcgtgctta gcgacgtcaa
     3061 catgccaaat atggatggtt accgcttgac gcaacgcatt cgtcagttgg gactgacgtt
     3121 gccggtaatc ggagtaactg ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc
     3181 cggtatggac agctgcctgt cgaagccggt aacgctggat gtgataaaac agacgctgac
     3241 ggtatatgcc gagagggtca ggaaatcgcg ggaatcgtag gacggataag gtgtttatgt
     3301 cgcatctggc attcagtgca aatgccagat gcgacgctga cgcgtcttat ctggcctact
     3361 ttgatgtaat ctgttgaaat aatgggaatc gtaggacgca taaggcgttt acgccgcatc
     3421 cggcaatcgg tgcaaatgcc agataagaca ctaacgcgtc ttatctggcc tacaggtgat
     3481 tagtctttat ctgccggact taacgtcact gaagagagat aattcagcag ggcgatatcg
     3541 ttctcgacac ccagcttcat catcgcagat ttcttctggc tactgatggt tttaatactg
     3601 cggttcagct ttttagcgat ctcggtcacc aagaagcctt ccgcaaacag gcgcagaact
     3661 tcactctctt ttggcgagag acgcttgtca ccgtaaccac cagcactgat tttttccaac
     3721 aggcgagaaa cgctttccgg ggtaaatttc ttccctttct gcagcgcggc gagagctttc
     3781 ggcagatcgg tcggtgcacc ttgtttcagc acgatccctt cgatatccag atccaatacc
     3841 gcactaagaa tcgccgggtt gttgttcata gtcagaacaa tgatcgacag gcttgggaaa
     3901 tggcgcttga tgtacttgat taaggtaatg ccatcgccgt acttatcgcc aggcatggag
     3961 agatcggtaa tcaacacatg cgcatccagt ttcggcaggt tgttgatcag tgctgtagag
     4021 tcttcaaatt cgccgacaac attcacccac tcaatttgct caagtgattt gcgaataccg
     4081 aacaagacta tcggatggtc atcggcaata attacgttca tattgttcat gtaataggct
     4141 accttgctac agcaagctct tgacataact gtcaatgtcg ctgatgtatt tttctattcc
     4201 tggaacatcc ttctcacgaa tcagatgttc cagcgtttca cataactgct tgccgggtac
     4261 cagatttagc atggcaaata cgcctttaag acgatgagcc gtttgggcta acgcagcaaa
     4321 gtcactggtt gctgcttcag tatacagcct cttaacatca tccggtactg tgtctacaaa
     4381 gagcgcataa tagccgctgg catggagttg cgcattttca tctccgccca gaggcgattc
     4441 tgtcacctct tcctgcgcca gttgcacttc aattaattgt aagaccgctt cctgcatagc
     4501 gttgctcata ttgaagttga cgcacaattg accaagccca atttcccgta cgccagactc
     4561 atcatcgctt aaaagcaagc cagaggcagt aagattagac ggattatccg ttaaaaagat
     4621 atcataatct tgactaatta atctttcatc gggtgtgata caggttgcac cccaattttc
     4681 taactggcga gtgacaatat tccgaatttc tgccgaagta acatccacca ttacgcagac
     4741 atcatccagt aaacgctctt cttcctcttc aacttccggg tcagcggcga gcattttgat
     4801 atgcacagag tagcgtgtac caagcccatc ccgcgttttg atgtttaaat gaccgcccag
     4861 tttacgcgcc agttgatcgc tcagccagaa tgccagcggg tccgccttgc catagcgatc
     4921 gttttggggc tggttgataa acgggaagtg caaattatcc atttcatgaa tacttacgcc
     4981 ttctcccgtg tccagaatgc ggaatgtcag gcggtcttca gaggactcat cctgatcaac
     5041 ctcaagggtg atttttccca attgcgttga ggtcacggca tattgcatca gtagcagcaa
     5101 aatacgtcgt aaggcatcgc gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt
     5161 aatcagcagt tgcagacctt tacgcttgat ggcaggcaac actgaaggca caacttcatc
     5221 aattaaatcc tgcacggaga acagcaccgt ctcacttttc cagctatcgt ccgcaagcat
     5281 gttcgctaac tgtatttcat cgaccagccg caccagcaca tctgcctgat tcgccagttg
     5341 tttgctttcc ggggcgttga gtttagccgc gctctccgcc agggactgtg cgggttcttt
     5401 cagcgcatcg ccaatgtttt tcataaaggt catccgcccc tgctggtttt tctcatacag
     5461 acgctgcgcc tgcttgagtt tcttgtttac cagcacttcg cgatcctgat cgcgaataat
     5521 gaaaatttgt gtgcgcggcg cgacctggct gcggaacatg cggatctcat acagctcgtt
     5581 attgatcgtc gcctgaataa tcccctgatg ctgttccgcc atggtggtga tgttttgcag
     5641 attcaaatgc ggcagcaaat gatcggcaat tttgttactt atgacagtgc ggttcgattc
     5701 ctgatcgtga accagcaggc cgagcggcag cagtgagact atctcttcat taattgcccg
     5761 taaaatgcgc aattcgttat tgaccgccgt gctgggtaga ctttctgtac tgcggctgga
     5821 gaaatggcgg aatgtggtat agccaaataa cgccagcgcc agcaaaccga tgttcagcag
     5881 cagtggcagc agaatgtttt gcaacgtatc cagcaataag gtgccataag gaacctgcca
     5941 gaccaggcgc atatcggtag agttgagtgc cgaggagatt tcaatcttcg tactgttaaa
     6001 gtggatactg acgctatccg tcccttcttt ctcattatca ttgtctcccg ttgccgtcgc
     6061 gtctggctca aggcggaaac tgtccagcgg catacccggt gggatcaaat cattaatcgg
     6121 tagatcaaaa gctacgaccg ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt
     6181 aaagtaatga ccgttctgcc aggccaggcg gcgcatgtta gaaaaacttt cgcgtgcatc
     6241 gagggcgttg gcctgttgca acatctctgc acgacgtgaa tcaacaatgt cgctgacggt
     6301 cgattcttta aatccggagg tgagatcttt gaggggcagg gttgagatca gcaccagact
     6361 gttatcctga ccattcaggt aatacatcga ccacggtaca ttttctgcgc cccacaatgt
     6421 gtccagatag gtggacatcc gctgagtcat ctcaagcgtt gagttgtcgt gagagccaaa
     6481 gattaacgct tccgttttgc ggcgcggttt ctccagatag tagacatcct gtttcaggcg
     6541 cgtctcttgt aaaccttcgc cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat
     6601 ctgccaggtc acgtaacgcc aggtattgac gcgcttttgg atagcgtggg taatgtcgac
     6661 aatctggtaa cttttatctt tcagccatgc gttaacggcg ctttgtacca ttacacccat
     6721 cgtcaccagt aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag
     6781 tgaaaagcgg gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa
     6841 acttcgcgaa gtgatagggc agtataaagg gtacagtgtg aaattccaga ctcttacctg
     6901 attgatgatt ctctattctc ccgtgatatt tatcatcctg cattggggga aatagatgta
     6961 caagcgccat tttgctgtac aaataaccta caaaaagccc aacgaaatta attgcaaata
     7021 aacgatgatt gctggaaatt atgctgatgt taattatttg tgaaatagtt aacaagcgtt
     7081 atagtttttc tgtggtagca cagaataatg aaaagtgtgt aaagaagggt aaaaaaaacc
     7141 ggatgcgagg catccggttg aaataggggt aaacagacat tcagaaatga atgacggtaa
     7201 taaataaagt taatgatgat agcgggagtt attctagttg cgagtgaagg ttttgttttg
     7261 acattcagtg ctgtcaaata cttaagaata agttattgat tttaaccttg aattattatt
     7321 gcttgatgtt aggtgcttat ttcgccattc cacaataatc ttaaaaagtt cccttgtatt
     7381 tacattttga aacatctata gcgataaatg aaacatctta aaagttttag tatcatattc
     7441 gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta
     7501 atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg
     7561 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct
     7621 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg
     7681 cactatttct ctgacaacaa gtcagaagac ggcgaccaga cctatgtacg tcttggtttc
     7741 aaaggcgaaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc
     7801 cagggcaata cctctgaaga caacaaagaa aactcctgga cccgtgtggc attcgcaggt
     7861 ctgaaattcc aggatgtagg ttctttcgac tacggtcgta actacggcgt tgtttacgac
     7921 gtaacttcct ggaccgacgt actgccagaa ttcggtggcg acacctacgg ttctgacaac
     7981 ttcatgcagc agcgtggtaa cggcttcgcg acctaccgta acaccgactt cttcggtctg
     8041 gttgacggtc tgaactttgc tgttcagtac cagggcaaaa acggtagcgt aagcggcgaa
     8101 ggcatgacca acaacggtcg tggcgctctg cgtcagaacg gcgacggtgt cggcggttct
     8161 atcacttatg attacgaagg cttcggtatc ggtgctgcag tttccagctc caaacgtact
     8221 gatgatcaaa atggtagcta catcagcaat ggtgtagttc gtaactacat cggtactggc
     8281 gaccgtgctg aaacctacac tggtggtctg aaatacgacg ctaacaacat ctacctggct
     8341 gctcagtaca cccagaccta caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa
     8401 gcacagaact tcgaagctgt tgctcagtac cagttcgact tcggtctgcg tccgtctgta
     8461 gcatacctgc agtctaaagg taaaaacttg ggtgtcatca atggtcgtaa ctacgacgac
     8521 gaagatatcc tgaaatatgt tgatgttggc gcgacctact acttcaacaa aaacatgtcc
     8581 acctatgttg actacaaaat caacctgctg gacgacaacc agttcactcg tgacgctggc
     8641 atcaacactg ataacatcgt agctctgggt ctggtttacc agttctaatc tcgattgata
     8701 tcgaacaaag ggcctgcggg ccctttgttc attgttttca gcgtacaaac tcagtttttt
     8761 ggtgtactct tgcgaccgtt cgcatgagga taatcacgta tggaaataag ctttacccgc
     8821 gtggcactgc tggctgccgc gctcttcttt gttggttgcg atcaaaaacc acaacccgcc
     8881 aaaacccacg ctactgaagt taccgttctt gaaggcaaaa ccatgggtac cttctggcgt
     8941 gccagcatcc cgggcattga tgccaaacgc agtgtcgaac ttaaagaaaa gattcagacc
     9001 cagctggacg ccgacgatca gctgctttcg acctataaaa aagattccgc gctgatgcgc
     9061 tttaacgact cgcaaagttt gtcgccgtgg ccagtaagtg aagcgatggc cgaaatcgtc
     9121 actacctcgc tgcgcattgg cgcgaagacc gatggtgcga tggatattac cgtcgggccg
     9181 ctggtaaatc tgtggggctt tggcccggaa caacagccgg ttcaaattcc gagccaggaa
     9241 cagatcgacg agatgaaagc caaaactggt ttacagcacc ttacggtcat taatcagtcg
     9301 catcagcaat atctgcaaaa agacctgccg gatttatatg tcgatctctc taccgtcggc
     9361 gaaggttatg cggcggatca cttggcacgc ttgatggagc aggaagggat ttcccgctat
     9421 ctggtgtcgg tgggcggcgc gctgaacagc cgtggtatga acggtgaagg cctgccgtgg
     9481 cgggtagcga ttcaaaaacc aaccgataaa gaaaacgcag ttcaggctgt ggtggatatc
     9541 aacggtcatg ggattagcac ctctggcagc taccgtaact attacgaact ggacggcaaa
     9601 cgtctttccc atgttatcga tccgcaaacc gggcgtccca tcgaacataa tctggtatcc
     9661 gtgacggtga ttgctccgac ggcgctggaa gccgatgcct gggatactgg cttgatggta
     9721 ctcgggccgg agaaagccaa agaggttgtt cgccgggaag ggctggcggt ttatatgatc
     9781 accaaagaag gcgatagctt taaaacctgg atgtcaccac agtttaaaag cttccttgtc
     9841 agcgaaaaaa attaaagcgc aagattgttg gtttttgcgt gatggtgacc gggcagacta
     9901 aaggctatcc ttaaccaggg agctgattat gaaaaacgcc acatgcttaa ctgacgatca
     9961 acgctggcaa tctgtcttag cccgcgaccc gaatgccgac ggcgaattcg ttttcgccgt
    10021 gcgcaccaca ggcatctttt gccgtccgtc ttgccgcgcc agacatgcct tgcgggaaaa
    10081 tgtctccttc tacgcaaatg ccagcgaggc actcgccgcc ggatttcgcc cctgcaaacg
    10141 ctgccaacct gataaagcca atgcccagca acatcggttg gataaaatca cccacgcgtg
    10201 tcgactgctg gaacaggaaa cgcctgtaac gttggaagcc ttagccgacc aggtggcgat
    10261 gagtccattt catctacatc ggttgtttaa agcgactacc ggaatgacgc ctaaagcctg
    10321 gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg agagcgtgac
    10381 gacgtctatt cttaacgccg gattccccga cagcagcagt tactaccgca aagccgatga
    10441 aacgctgggc atgacggcta aacaattccg tcacggtggc gaaaatctgg cggtgcgtta
    10501 cgcgctggct gattgtgcgc tgggtcgttg cctggtggca gaaagcgagc gggggatttg
    10561 cgcgatattg ctgggcgatg atgacgcgac attaatcagc gagttgcagc agatgtttcc
    10621 cgctgccgac aacgcgcctg ccgatctgac gtttcagcaa catgtgcgtg aagtgatcgc
    10681 cagcctcaat caacgcgata cgccgctgac gttaccgctg gatattcgcg gcactgcttt
    10741 tcagcaacaa gtctggcagg cactgcgcac gataccttgc ggtgaaaccg tcagttatca
    10801 gcaactggct aacgccatcg gcaaaccgaa agcggtacgg gccgttgcca gcgcctgtgc
    10861 cgccaacaag ctggctatcg taataccctg tcatcgagtg gtccgtggtg atggcacact
    10921 ttccggttac cgctggggcg tgtcgcgtaa agcgcaactg ctgcgccgcg aagctgaaaa
    10981 tgaggagagg taatgttgga tctgtttgcc gatgctgaac cgtggcaaga gccactggcg
    11041 gctggtgcgg taattttacg gcgttttgct tttaacgctg cggagcaact gatccgcgat
    11101 attaatgacg ttgccagcca gtcgccgttt cgccagatgg tcacccccgg gggatatacc
    11161 atgtcggtgg cgatgaccaa ctgtgggcat ctgggctgga cgacccatcg gcaaggttat
    11221 ctctattcgc ccattgatcc gcaaacaaat aaaccgtggc ccgccatgcc acaaagcttt
    11281 catgatttat gtcaacgtgc ggctacggcg gcgggctatc cagatttcca gccagatgct
    11341 tgtcttatca accgctacgc tcctggcgcg aaactgtcgc tgcatcagga taaagacgaa
    11401 ccgaatctgc gcgcgccaat tgtttctgtt tctctgggct tacccgcgat ttttcaattt
    11461 ggtggcctga aacgtaatga tccgctcaaa cgtttgttgt tggaacatgg cgatatggtg
    11521 gtatggggcg gtgaatcgcg gctgttttat cacggtattc aaccgttgaa agcggggttt
    11581 catccactca ccaccgactg ccgctacaac ctgacattcc gtcaggcagg taaaaaagaa
    11641 taaaaataag aattattatt gctgtgcgcg cgaagatgtt taaactgcgg gctgtaattc
    11701 attgtccggg ttttctgcat ggaacttctt gtacttgtct ggcggcagta tcgctggcca
    11761 tttatcagtg tgatggcgct aagcctcgcc agtgcggcat taggcattgg cttaattgct
    11821 tttatcaatc agcgccttat cgaaatggcg gataccagtc tgctggtatt gccggagttt
    11881 ctgggattat tgctgctgct gatggcagtc actctcggat cgcaactggc gctcaccact
    11941 ttggggcatc acttcgttta ccgactgcgt agtgaattta tcaagcggat tctggatacc
    12001 cacgtcgagc gcattgaaca actcggtagc gcctcgttgc tggcgggatt aaccagcgat
    12061 gtgcgcaata tcaccattgc ttttgtgcgt cttccggaac tggtgcaggg gatcattctc
    12121 actatcggtt ccgcggcgta tctgtggatg ctgtcgggca aaatgttgct ggtaacggct
    12181 atctggatgg cgatcaccat ctggggtggt tttgtactgg tggcgcgggt gtacaaacat
    12241 atggcgaccc tacgtgaaac cgaagacaag ctgtacacgg attttcaaac tgtacttgaa
    12301 gggcgcaaag agctgactct gaaccgggaa cgcgccgagt atgtgtttaa caacctctac
    12361 atccccgatg cgcaagagta tcgccatcat attattcgcg cagacacctt ccatcttagt
    12421 gccgtgaact ggtcaaacat catgatgctg ggcgctatcg gcctggtgtt ctggatggcg
    12481 aacagtctcg gttgggctga taccaacgtt gctgcgacct attcgttgac gcttttattc
    12541 ctgcgtacgc cgctgctttc ggcggttggc gcattgccga cgctgctgac ggcgcaggtg
    12601 gcgtttaaca agctgaacaa attcgcgctc gcgcctttca aagcagagtt tccgcgcccg
    12661 caggcgtttc ccaactggca aacgctggag ctgcgtaatg tgacgtttag ctatcaggat
    12721 aacgcgtttt ccgttggtcc gattaatctc accatcaaac gtggcgagct actgtttctg
    12781 attggtggta acggtagtgg aaaatcgacg ctggcgatgt tgttgacggg cttgtatcag
    12841 ccacaaagcg gcgaaatctt gctggatggc aaacctgtca gcggcgaaca accggaagat
    12901 tatcgcaaac tgttttcggc agtgtttacc gatgtctggc tgtttgatca actgctgggg
    12961 ccggagggta aacccgctaa cccgcaactg gttgagaagt ggctggcgca gctgaaaatg
    13021 gctcataagc ttgagttaag caacgggcgt attgttaacc tgaagttatc aaaagggcag
    13081 aaaaaacgcg tggcgctgtt gctggcgctg gcagaagaac gcgatattat cctgctggat
    13141 gaatgggcgg cggatcagga tccacacttc cgtcgtgagt tttatcaggt gttgctgccg
    13201 ctgatgcagg agatgggtaa aactattttc gctatcagtc atgatgatca ttactttatc
    13261 cacgccgacc gcctgctgga aatgcgcaat gggcaactta gcgagctgac gggcgaagag
    13321 cgcgatgccg cttcgcgtga tgccgttgcc cggacggcat aatattacga cgctgacctg
    13381 gcggactgct gccgtcaggt caatatgcta aaaatccctt catttctaat aaccctataa
    13441 ttaatcaacg aaattataat gtttctaaaa ttagaatata atttataaac attatttaaa
    13501 tgttgttact taagtgttac ctttgatgcc gcgcaaatct cacctgcaat aaagcgacta
    13561 aaagtaaggc attaacaaga tgaaaaaagt gactgccatg ctcttctcga tggccgtggg
    13621 gcttaatgcc gtttcgatgg cggcaaaagc gaaagcgtcc gaggagcagg aaactgatgt
    13681 actgttgatt ggcggcggca ttatgagcgc cacgttgggg acctatttac gcgagctgga
    13741 gcctgaatgg tcgatgacca tgctggagcg cctggagggt gtcgcgcagg agagttcgaa
    13801 cggctggaat aacgccggaa ccgggcattc tgcactgatg gaactgaact acaccccgca
    13861 aaacgccgat ggcagtatca gtattgaaaa agcagtcgcc attaacgaag catttcagat
    13921 ttcccgccag ttctgggcgc accaggtcga gcgcggcgtg ctgcgtactc cgcgttcatt
    13981 tatcaatacc gttccgcata tgagctttgt ctggggcgag gataacgtta atttcctgcg
    14041 cgcccgttac gccgcgttgc aacaaagctc gctgtttcgc ggtatgcgtt actctgaaga
    14101 tcacgcgcag atcaaagagt gggcaccgtt agtgatggaa gggcgcgatc cgcaacagaa
    14161 agtggcagcc acgcgtacgg aaattggtac cgatgtgaac tacggcgaga tcacccgcca
    14221 gttaattgct tccttgcaga agaaatctaa cttctcgctg caactcagca gcgaagtccg
    14281 cgccctaaag cgtaatgacg ataacacctg gaccgttacc gttgccgatc tgaaaaatgg
    14341 cactgcacag aacattcgtg ccaaatttgt ctttatcggc gcgggcggtg cggcgctgaa
    14401 gctgttacag gaatcgggga ttccggaagc gaaagactac gccggtttcc cggtgggcgg
    14461 acagtttctt gtttcggaaa acccggacgt ggttaatcac catctggcga aggtttacgg
    14521 taaagcatcc gttggcgcac caccgatgtc ggttccgcat atcgataccc gcgttctgga
    14581 cggtaaacgc gtagtgctgt ttgggccatt tgccaccttc tcaaccaaat tcctcaaaaa
    14641 cggttcattg tgggatctga tgagttccac caccacctct aacgtgatgc cgatgatgca
    14701 cgtcgggctg gataatttcg atctggtgaa atatctggtg agtcaggtga tgttgagtga
    14761 agaggatcgt tttgaagcgt tgaaagagta ctatccgcag gcgaaaaaag aggactggcg
    14821 tttgtggcaa gcggggcagc gtgtgcagat tatcaagcgt gatgccgata aaggtggtgt
    14881 actgcgtctg ggtactgaag tggtcagtga ccagcaaggg accattgccg cgctcctggg
    14941 agcatcgcca ggggcgtcaa ccgccgcgcc gattatgttg gatctgctgg aaaaagtatt
    15001 tggcgatcgc gtttccagcc cgcaatggca ggctacgttg aaagcgatcg ttccgtctta
    15061 tggacgcaag ctgaacggtg atgtagcggc aacagaacgc gagttgcagt acaccagcga
    15121 agtgctgggg ctgaaatacg acaagccgca ggcagcagat agtacgccaa aaccgcagtt
    15181 gaaaccgcaa cccgttcaaa aagaagtggc ggatattgcg ttgtaatgat gtgccacatc
    15241 cggcatggta tgccggatgt ggcgtatact gataagacgc gccagcgtcg catcaggcaa
    15301 ccggctcggg cgttagatgt tggatgcggc gtaaccgcct tatccaacca acggggtgcc
    15361 gtacttcaca acagtttagc gaacttctgc gttgtcaatt ttctcttccg ccttccagac
    15421 gcggtatttc acatctacat tgtctggcgt atacaccacg atcggcagct tgctgttgta
    15481 acgcagcatt ccagcatcgc ccagatacgc ggtgacaaat ttcttctctt tcttgccatc
    15541 cgggcaggcc atcatcgtgg aaaccggcga gctaacttta tcaaagacat aataatcata
    15601 gccccggcct tccagcgttt tgctttccag cttcccgccg agacgatgca aattgcaatc
    15661 gacttccagc gtctgaccga ttaacagttc tactttcagg ggagattcat cttcttgcgg
    15721 ggttaactga atcacctgac gcttcatccc tttttcagct tgtggataag gcgcgatttt
    15781 ttccagtggc tggacgcttt ctgccgccca ggcggaagtg gtagcgaaag cggcaaacaa
    15841 tactgcaggt agaatggtct tcattttttt gctttccttc ttcatttata ggttttcacc
    15901 gatgatgtcg ctgaaggtta acatattcat aagatttatt aatttgcttt atgaataact
    15961 taaagaatat tcagacaatt ttacctgctc ttttagtgtt aaattctgat gagagaacaa
    16021 accagcagaa atatctctgg tgaattaatt gctatataaa tatgtttata accatttgaa
    16081 atgtgagcaa aagcccgttt ttgccacgct tcccgccact cttttgatcc tgctacaggt
    16141 tttaccccga tcggggtatg catctttgac acatccttta atatcttagc ggttataaaa
    16201 atggcttatt aattatgcgg tttatttggt cgctctcaat tttcagagcg cgttaatgat
    16261 ggaaggtcaa tgtgaagatt gatgcatccc gtcggggcat actcactggt cgctggcgca
    16321 aagccagtaa cggtatccgt ccgccctggt ccggtgatga atctcatttt ctgacccatt
    16381 gcacccgttg cgacgcctgt attaatgctt gtgaaaacaa cattctgcaa cgcggcgcgg
    16441 gcggctatcc gagcgttaat ttcaaaaata acgagtgcag cttctgttac gcttgcgcgc
    16501 aggcttgccc cgaatcgcta ttttctccgc gccacaccag ggcctgggat ttgcagttca
    16561 ccattgggga cgcgtgcctg gcgtatcagt cagttgaatg ccgccgctgt caggatagct
    16621 gtgagccaat ggcaattatc tttcgcccga cgctctccgg gatctaccag ccgcaactta
    16681 atagccaact ttgcaacgga tgtggagcat gtgcagccag ctgtccggta tcagccataa
    16741 ctgcggagta tctccatgca cactaactgg caagtttgca gcctggtcgt gcaggccaaa
    16801 agcgaacgaa tttcagacat cagcacccaa ctgaacgcct ttcccggctg tgaagttgct
    16861 gtcagcgacg cgccgagcgg tcagttgatt gtggtggtgg aagcagaaga cagcgaaacg
    16921 ctgatccaaa ccattgagtc agtacgcaac gtagagggcg tgctggcggt gtcgctggtt
    16981 tatcaccagc aggaagagca aggtgaggaa acaccatgaa actcagtcgt cgtagcttta
    17041 tgaaagctaa cgccgttgcg gccgctgcgg cggctgccgg tctcagcgtg ccgggcgttg
    17101 cccgcgccgt tgttggtcag caggaagcca tcaaatggga taaagcgccg tgccgtttct
    17161 gcggtactgg ttgcggcgtt ctggtcggaa cgcagcaggg acgtgtggtg gcctgtcagg
    17221 gcgacccaga cgcaccggtt aaccgtggcc tgaactgcat taagggctat ttcctgccca
    17281 agatcatgta cggtaaagac cgtttgacgc agccgctgct gcgtatgaaa aacggtaaat
    17341 atgacaaaga aggcgaattt accctaatca cctgggatca ggccttcgat gtgatggaag
    17401 agaaattcaa aaccgccctg aaagaaaaag ggccggaatc gatcggtatg ttcggttctg
    17461 gtcagtggac tatctgggaa ggttatgccg cgtccaagct gtttaaagcg ggcttccgtt
    17521 cgaacaacat cgacccgaac gcgcgtcact gtatggcgtc ggcagtagtt ggctttatgc
    17581 gtacctttgg tatggatgag ccgatgggct gctatgacga catcgagcag gctgacgcgt
    17641 ttgtgctgtg gggcgcaaac atggcggaga tgcacccgat cctctggtcg cgcatcacta
    17701 accgtcgtct ctctaatcag aacgtcaccg tggcggtgct ttctacctac cagcatcgta
    17761 gcttcgagct ggcggataac ggcatcatct ttacgccaca gtctgacctg gtgatcctga
    17821 actacatcgc caactatatc attcaaaaca atgcgataaa tcaggacttc ttcagcaaac
    17881 acgttaacct gcgcaaaggg gcgacggaca ttggctacgg tttacgtccg acccatccgc
    17941 tggaaaaagc agcgaagaat ccaggttctg acgcctccga accgatgagc tttgaagatt
    18001 acaaagcctt cgttgccgag tatacgctgg aaaaaactgc cgaaatgacc ggcgtgccga
    18061 aagaccagtt agaacaactg gcgcagctgt atgccgatcc gaacaagaaa gtcatctcct
    18121 actggacgat gggcttcaac cagcatactc gtggcgtgtg ggctaacaac ctggtctaca
    18181 acctgcacct gctgaccggc aaaatttccc agccgggttg cggtccgttc tccctgaccg
    18241 ggcaaccttc tgcgtgtggt actgcgcgtg aagtgggcac ctttgctcac cgtctgcctg
    18301 cggacatggt ggtgaccaac gagaaacacc gtgatatctg cgagaagaag tggaatatcc
    18361 cgagcggcac cattccggcg aaaatcggtc tgcacgcggt ggcacaagac cgtgcgctga
    18421 aagacggcaa actgaatgtt tactggacca tgtgtaccaa caacatgcag gccgggccga
    18481 acatcaatga agagcgtatg ccgggctggc gcgatccgcg caacttcatc atcgtctccg
    18541 atccgtatcc gacagtcagt gcgctggccg ccgacttgat cctgccgacc gcaatgtggg
    18601 tagagaaaga gggcgcttac ggtaacgccg aacgccgtac tcagttctgg cgtcaacagg
    18661 tacaggcacc gggcgaagcg aaatcggatc tctggcagtt agtccagttc tcccgccgct
    18721 tcaaaactga agaagtatgg ccggaagatc tgctggcgaa gaaaccggaa ctgcgtggca
    18781 aaacgctgta cgaagtgctg tatgcaacac cggaagtgag caaattcccg gtatccgaac
    18841 tggcggaaga tcagctgaac gatgaatccc gcgagctggg cttctatctg caaaaagggc
    18901 tgttcgaaga gtacgcatgg tttggtcgcg gtcacggtca cgatctggca ccgttcgatg
    18961 actaccacaa agcgcgcggt ctgcgctggc cggtggtgaa cggtaaagaa acgcagtggc
    19021 gttacagcga aggtaacgac ccgtacgtga aagcgggcga aggctataag ttctacggca
    19081 aaccggatgg caaagcggtg atcttcgcac tgccattcga accggcggcg gaagcaccgg
    19141 atgaagagta cgacctgtgg ctctctaccg gacgcgttct ggagcactgg cacaccggca
    19201 gtatgactcg ccgtgtacca gaactgcacc gcgccttccc ggaagcggtc ctgtttattc
    19261 acccgctgga tgcgaaagcg cgcgatctgc gtcgtggcga caaagtgaaa gtggtttctc
    19321 gccgtggcga agtgatctcg attgtcgaaa cgcgcggtcg taaccgtccg ccgcagggcc
    19381 tggtgtacat gccgttcttc gacgccgcac agctggttaa caaactaacg ctggacgcga
    19441 ccgatccgct ctcgaaagag acggacttca agaagtgcgc agtcaaactg gagaaggtgt
    19501 aagccattat gtcccggtca gcgaaacctc aaaatggtcg ccgccgcttt ctgcgcgatg
    19561 ttgttcgcac agcaggcggg ctggctgccg tgggtgtggc gctggggtta caacagcaaa
    19621 ccgcacgcgc atctggcgtg cggttgcgcc cgcccggagc cataaacgag aacgcctttg
    19681 ccagtgcctg tgtgcgttgc ggtcagtgtg ttcaggcttg cccttacgac accttaaaac
    19741 tggcgacgct ggcctctggt ctgtcggcgg gcacgccata ttttgtcgca cgggatattc
    19801 cttgcgaaat gtgtgaggac attccgtgcg ccaaagtgtg cccgagcggt gcgctggatc
    19861 gtgagatcga atcgatcgac gacgcgcgga tggggctggc ggtactggtg gaccaggaaa
    19921 actgtctcaa ctttcagggg ctgcgctgcg atgtttgtta tcgcgaatgc ccgaaaattg
    19981 atgaggccat caccctggag ctggagcgca acacgcgtac cggtaagcac gcccgctttc
    20041 tgccgacggt tcacagagac gcctgtaccg gttgcggtaa gtgcgaaaaa gtgtgcgtgc
    20101 tggaacaacc ggcaatcaag gtgttaccgc tgtcactggc taaaggggag ttaggtcacc
    20161 attaccgctt cggctggctg gaggggaaca atggcaaatc gtaaacgtga cgccgggcgc
    20221 gaggcgctgg agaaaaaagg ctggtggcgc agtcaccgtt ggctggtgtt acgtcgcctt
    20281 tgtcagttct tcgtgctggg gatgtttttg agcggtccgt ggtttggtgt gtggatctta
    20341 cacggtaact acagcagtag cctgttattc gacaccgttc cgctgaccga tccgctgatg
    20401 acgctgcaaa gtcttgccag cggtcatctg cctgccacgg tggcgttgac cggtgcggtg
    20461 attatcaccg tgctctacgc cctggcagga aagcgattat tttgcagctg ggtctgcccg
    20521 ctgaatccga ttaccgactt agcgaactgg ttacgcaggc gctttgacct caatcagtct
    20581 gcgacgatcc ctcgccacat tcgctacgtg ctgttggtgg tcattctgct gggatcggcc
    20641 ttaactggca cgctcatctg ggaatggatc aaccccgtat ctctgatggg gcgcagcctg
    20701 gtaatgggct tcggcagcgg cgcgctgctt attctcgcgc tgtttttatt tgatctactg
    20761 gtcgttgaac acggctggtg cgggcacatt tgtccggttg gcgcgctgta tggcgtgctg
    20821 ggtagcaaag gtgtgattac cgttgcggcc accgatcggc agaaatgtaa ccgctgtatg
    20881 gattgttttc atgtttgccc ggaaccgcgt gtgctacgtg ccccggtgct ggatgaacaa
    20941 agcccggtgc aggtcaccag ccgcgattgc atgacgtgcg gtcgctgcgt ggatgtctgt
    21001 tctgaggatg tatttacaat aactacacga tggagttcgg gagcgaaatc atgaaaagcc
    21061 atgacctgaa gaaagcgctg tgtcaatgga cggcgatgct ggccctggtg gtaagcggcg
    21121 cggtttgggc ggctaatggc gtcgatttta gccagtcgcc ggaagtatcc gggacacagg
    21181 aaggggccat tcggatgcca aaagagcagg aacggatgcc gctgaactat gtgaatcagc
    21241 cgccaatgat cccgcatagc gttgaaggtt atcaggtaac gaccaatacc aaccgctgct
    21301 tgcagtgcca cggtgtcgaa agttatcgca ccactggcgc gccgcgcatt agtcctactc
    21361 actttatgga cagcgacggc aaagtgggcg cggaagtggc tccgcgtcgt tatttctgtc
    21421 tgcaatgtca cgtaccgcag gccgataccg cgccaatcgt ggggaatacc tttaccccat
    21481 caaaaggtta cgggaaataa gaggttatta tgggaaattc tgaccgtaag cctggtctga
    21541 ttaagcgcct gtggaaatgg tggcgtaccc ccagccgtct ggcgctgggg acgctgctgt
    21601 tgatcggttt tgttggcggc atcgtcttct ggggtggctt taacaccggg atggaaaaag
    21661 ccaataccga agagttctgc attagctgcc acgaaatgcg caacacggtg tatcaggaat
    21721 acatggattc cgtgcactac aacaaccgta gcggcgtccg tgcgacctgt cgggattgtc
    21781 acgttccgca cgagtttgtg ccgaagatga tacgcaagct caaagcaagt aaagagctgt
    21841 atggtaaaat ttttggcgtt attgacactc cgcagaaatt tgaagctcat cgtctgacga
    21901 tggcacagaa tgagtggcgg cgcatgaagg acaataactc gcaggagtgc cgtaactgtc
    21961 acaacttcga gtatatggat acaaccgccc agaaatcggt tgccgcgaag atgcatgacc
    22021 aggcggtgaa agatgggcaa acctgtattg attgccataa agggatagcg cacaagctgc
    22081 ccgatatgcg tgaagtcgag ccaggttttt aacagggtta ttgcgtgggt atgcttgaag
    22141 ccagagagtt actttgtgag cgggatgaac gaaccttatt tagtggcttg tcatttacgc
    22201 tgaacgcagg agagtgggta caaatcaccg gtagcaacgg cgcggggaag acaacgcttc
    22261 tccgtttgct gacggggttg tctcgccctg acgcaggcga ggttctctgg caaggacagc
    22321 ccttgcatca ggtacgcgac agctaccatc aaaacctgtt atggataggc catcagccgg
    22381 ggatcaaaac ccggctgacg gcgttagaaa atctgcactt ttatcatcgc gatggcgata
    22441 ccgcacaatg tctggaagcc ctggcgcagg ccgggcttgc cggattcgaa gatattcctg
    22501 taaatcagct ctcggccggg caacaacgcc gcgtcgcttt agcgcgtctg tggctgaccc
    22561 gtgccacgtt atggatcctc gacgagcctt ttaccgcgat tgacgttaac ggcgtcgatc
    22621 gtctgaccca gcgtatggcg cagcatacgg agcagggggg gattgtgatt ctgactaccc
    22681 accagccgct caacgttgct gaaagtaaaa ttcgccgcat ttcactgacg caaacgaggg
    22741 ccgcatgatg ttctggcgca ttttccgtct tgagctgcgt gtagcgtttc gccatagcgc
    22801 cgaaatcgcc aacccgctgt ggttcttcct gattgtaatt accctttttc cgctcagtat
    22861 cggtccggag ccgcaactgc tggcgcgtat tgcaccgggc attatctggg ttgctgcgct
    22921 gctttcatcc ttgctggcgc tggaacgact gttccgtgac gatttgcagg acggcagtct
    22981 tgaacaattg atgttgttgc cgttaccctt gcccgccgtt gtgctggcga aggtgatggc
    23041 gcactggatg gtaaccggtc tgccgttact catcctttcg ccactggtag caatgctact
    23101 gggaatggat gtttatggct ggcaagtgat ggcgctgacg ctgctgctgg gaacgcctac
    23161 gcttggcttt ctcggtgcac cgggtgtggc gttgacagtg ggacttaagc gcggtggtgt
    23221 gctgctcagc atactggtgt taccgctgac tatcccatta ctcatctttg ccaccgccgc
    23281 gatggacgcg gcttccatgc atttgcccgt tgacgggtat ctggcaattt taggcgcgtt
    23341 gctggcaggc accgcgacat taagtccttt tgcgacggcg gcagcgttac gaatcagcat
    23401 tcaataacgc tggattactg atttttgttc gagtctggta tcgaaactat gtggaaaaca
    23461 ctgcatcaac tggcgatccc accacggctg tatcaaatct gtggctggtt tataccgtgg
    23521 ctggcaattg ccagtgtggt cgtgcttacc gtcggctgga tctggggatt cggctttgct
    23581 ccggctgatt atcagcaggg aaatagctac cgcattatct acctgcatgt gcctgcggcg
    23641 atctggtcga tgggtattta tgcatcaatg gcggtggcgg cgtttattgg ccttgtctgg
    23701 cagatgaaaa tggccaacct ggcggtggcg gcgatggccc ccattggtgc cgtgtttacc
    23761 tttattgccc tggttaccgg ctctgcatgg ggaaaaccga tgtggggcac ctggtgggta
    23821 tgggatgcac gtctgacttc tgaactggtg ctgctgtttt tgtatgtggg tgtgattgcc
    23881 ctgtggcacg ccttcgacga ccgccgtctg gcgggccgtg cggcaggtat cctggtgctg
    23941 attggcgtgg tgaatctgcc gattattcat tactccgtgg agtggtggaa caccctgcat
    24001 cagggatcaa cgcggatgca gcaaagtatc gatccggcga tgcgttcgcc gctgcgctgg
    24061 tcgatttttg gcttcctgct cctgtctgcc acgctgacgc tgatgcggat gcgtaatttg
    24121 attttgctga tggaaaaacg ccgtccgtgg gtgagtgaac tgatactgaa aagaggccgt
    24181 aaatgacccc tgcatttgct tcctggaatg aatttttcgc aatgggcggt tacgcctttt
    24241 ttgtctggct ggcggtggtg atgaccgtta ttccgctggt ggttttggtc gtgcactcgg
    24301 tgatgcagca tcgcgcaatt ctgtgtggtg tggagcaaca gcgggcgcgt gaggcgcgtt
    24361 tacgtgctgc gcaacagcag gaggctgcat gaatattcgc cgtaaaaacc gcttgtggat
    24421 tgcctgtgct gtgttggcag gtttggcgct gaccatcggt ctggtgctgt atgcgctgcg
    24481 ctcgaatatc gatctctttt atacgccggg ggaaattctc tacggcaagc gtgaaactca
    24541 gcaaatgccg gaagtcggtc agcgtctgcg cgttggcggg atggtgatgc cgggtagtgt
    24601 gcagcgcgat cccaattcgc tgaaagtgac cttcaccatt tacgacgctg aaggctcagt
    24661 ggatgtctct tacgaaggca ttttgccgga tctgttccgt gaagggcagg gcgttgtggt
    24721 gcagggcgag ctggaaaaag gcaatcatat cctcgcgaaa gaagtgctgg cgaaacacga
    24781 cgaaaactac acgccgccag aagttgagaa agcgatggaa gctaaccacc gtcgcccggc
    24841 gagtgtttat aaggacccag catcatgatg ccggaaattg gaaacgggct gctgtgcctg
    24901 gcgctgggaa ttgcgctgct gctgtccgtg tatccgctat ggggcgtggc gcgcggagat
    24961 gcgcgcatga tggcgtcttc ccgcttgttt gcctggctgc tgtttatgtc tgtggctggc
    25021 gcatttctgg tgctggtcaa tgctttcgtg gtcaacgact ttaccgtcac ctatgttgcc
    25081 agcaactcca atacccagct tccggtgtgg tatcgcgtgg cggctacctg gggcgcgcat
    25141 gaaggttcgc ttctgctgtg ggtgctgctg atgagcggct ggacctttgc ggtggcgatt
    25201 tttagtcagc gtattccgct ggatattgtg gcccgtgtac tggcgataat ggggatggtc
    25261 agcgtcggct ttttgctgtt cattctcttt acctctaacc cgttctcacg cacgctgctg
    25321 aacttcccaa ttgaagggcg cgatcttaac ccgctgttac aggatccggg gctgatcttc
    25381 catccgcctc tgctttatat ggggtacgtg ggtttctcgg tggcgtttgc ttttgccatt
    25441 gcttctttgc tgagcgggcg tctggacagc acttatgcgc gttttactcg cccgtggacg
    25501 ctggcggcgt ggattttcct gacgctcggc attgtgctcg gttccgcatg ggcctattac
    25561 gaactcggct gggggggctg gtggttctgg gatccggtag aaaacgcctc gtttatgccg
    25621 tggctggtgg ggactgcgct gatgcactca ctggcggtca ctgaacaacg cgccagcttc
    25681 aaagcgtgga cattactgct ggcaatcagt gccttctcgt tgtgtctgct ggggactttc
    25741 ctggtgcgtt ccggcgtgct ggtatcggta cacgcgtttg cctctgatcc ggcacgcggt
    25801 atgtttatcc tcgcctttat ggtactggtg attggtggtt cgctgctgct gtttgccgcg
    25861 cgtggacaca aagttcgctc acgcgtaaac aatgcgctgt ggtcgcggga atctctgctg
    25921 ttagcgaaca acgttttgtt ggtcgccgcg atgctggtgg tattactggg gacgctgctg
    25981 ccgctggtgc acaagcaact gggactgggc agtatttcga ttggcgaacc gttcttcaac
    26041 accatgttta cctggctgat ggtgccgttt gcgctgctgc ttggtgtcgg tcctctagtg
    26101 cgctgggggc gcgatcgccc acgtaaaatc cgcaatttat tgattatcgc cttcatctca
    26161 acgctggtgc tgtcgctgct gctgccgtgg ctgttcgaaa gcaaagttgt ggcgatgacg
    26221 gtgctcggcc tggcaatggc ctgctggatt gctgtgctgg caattgcgga agctgcgctg
    26281 cgtatttcac gtggcacgaa aaccaccttc agttattggg gaatggtggc ggctcacctg
    26341 gggctggcag tgacaattgt tggtattgcc tttagccaga actatagcgt tgagcgtgat
    26401 gtgcgcatga agtccggcga tagcgtcgat attcatgaat atcgcttcac cttccgtgat
    26461 gtcaaagagg tgactggccc gaactggcgc ggcggtgtgg tgactattgg cgtaacgcga
    26521 gagggtaaac cggaaacggt gctgtatgcg gaaaaacgtt attacaacac tgccgggtcg
    26581 atgatgaccg aagcggcgat tgacggcggc atcacgcgtg acctgtacgc cgcgctcggt
    26641 gaagagctgg aaaacggcgc gtgggccgtg cgtctttact acaaaccgtt tgttcgctgg
    26701 atttgggcgg gcgggctgat gatggcgttg ggcggactgc tgtgtctgtt tgatcctcgc
    26761 tatcgtaagc gcgtgagtcc gcaaaaaact gcgccggagg ctgtatgaag cgcaaagtat
    26821 tgttaattcc gttgattatc ttcctggcga ttgccgcggc gctgctgtgg cagctggcgc
    26881 gtaatgccga aggggatgat ccgaccaatc tggaatcggc gctcattggc aagccagttc
    26941 cgaagtttcg cctcgaatca ctggacaatc cggggcagtt ttatcaggcg gatgtgctga
    27001 ctcagggcaa acctgtgctg cttaacgtct gggctacctg gtgtccgacc tgccgtgcgg
    27061 aacatcaata tctgaatcag ctttctgcgc agggcattcg cgtggtcggc atgaactata
    27121 aagacgatcg ccagaaggca ataagctggc tgaaagagct gggcaatcct tacgcgctaa
    27181 gcctgtttga tggcgatggc atgttagggc tggatctcgg tgtctatggc gcgccagaaa
    27241 cgttcctcat cgacggcaac ggcatcattc gctatcgcca tgcgggcgat ctcaatcctc
    27301 gcgtctggga agaagagatc aagccgctgt gggagaaata cagtaaggag gccgcacaat
    27361 gaggttttta ttgggcgtgc tgatgctgat gatctccggc tcagcgctgg cgaccatcga
    27421 cgtgttgcag tttaaggatg aagcgcagga gcagcagttc cgccaactca ctgaagaact
    27481 acgctgcccg aaatgccaga acaacagcat tgccgattcc aactcgatga ttgccaccga
    27541 cctgcgccag aaagtgtatg aactgatgca ggaaggtaaa agtaaaaaag agattgtcga
    27601 ttatatggtg gcgcgttacg gcaacttcgt cacttacgat ccgccgttaa cgccgctgac
    27661 cgtgctgctg tgggtgcttc cggtagtggc tattggcatt ggcggttggg tcatttacgc
    27721 ccgttcgcgg cgtcgggtac gcgtggtgcc ggacgcgttt cctgaacaaa gcgtgcagga
    27781 aggtaagcgt gccggatata ttgtttatct gccgggtatt gtggtggcgt taattgtggc
    27841 tggcgtcagc tactaccaga ctggcaatta tcagcaggtg aaaatctggc agcaggccac
    27901 ggcacaggct ccggcgttgc tggacagggc gctggatccg aaagccgatc cgctcaacga
    27961 agaagagatg tcgcgtcttg cgctggggat gcgtactcaa ctgcaaaaaa atccgggaga
    28021 tatagaaggc tggattatgt tgggccgcgt tggcatggcg ctgggtaacg ccagtatcgc
    28081 caccgatgca tacgctactg cgtatcgcct cgatccgaaa aacagtgatg ccgcactggg
    28141 ttatgctgaa gcgttgacac gttcatctga tcccaacgac aaccgcctcg gtggtgaact
    28201 gctgcgccag ttggtgagaa cggaccatag caatatccgt gtgttaagca tgtatgcgtt
    28261 taatgccttt gagcagcagc gatttggcga agccgttgcc gcgtgggaga tgatgttgaa
    28321 actcttatct gccaacgata ctcgccgtgc ggtgattgaa cgtagtatcg cgcaggcgat
    28381 gcaacatttg tcgccgcagg agagtaaata aaaagaggcc cgatggtgat gccatcgggc
    28441 tatttttatt gtgccccgcg ttgttgcagg aacagaatgg tggctgccac gcgtgagcgg
    28501 acattgagtt tgcgcagcag attgcgaata tgtactttta ctgtctgctc tgaaatattc
    28561 aacaccgagg caatctgttt atttgacagc ccctgtgcca gctcatgcag aacatccagc
    28621 tcgcgctccg tcagcacgct gaagggatct tcttccgcgc caaacatttc acgttcacgt
    28681 aagtactgat tgacgcgttc gctaaagact ttgctgcctt tcgctccagc acgaattgct
    28741 tccagcaata cttccgggtc gctgtctttc aacagataac cgtctgcgcc tgcgtctatc
    28801 agcgcaaaga catcgctgga ggcatctgat acggtcagga taataatttg cgcggtaacg
    28861 ccatccctgc gcaaggcatt gagggtatcc aggccactca tacctttcat attgagatcc
    28921 agcaagatca cgtcgatatc cagtctattc gccagatcga tcgcgctcgc gccgtcgccc
    28981 gcttcggcga ccacttcaaa gccaggatca agctccagta actgacgaac accgcgtcgc
    29041 ataagtggat gatcatccac aatcatcacc tgaaaaggcg ttgcttcagg catagtagtc
    29101 tcctggggtt ttattagaat cattatgttt gagcgtagcg ttggtaacgc taccaggcgc
    29161 gtgaatgtta ccccatttta tgtatggaca tgtagtgaaa actacaacct tgtcgttcgc
    29221 tttggcatca ttttcattat aaagacggaa ttttgcatca gcttctc
//
LOCUS       DAJTMK010000034        28636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTMK010000034 DAJTMK010000000
VERSION     DAJTMK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28636
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.035"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            46..957
                     /locus_tag="OO135_001272"
                     /pseudo
     CDS             46..957
                     /locus_tag="OO135_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1000..1860)
                     /gene="rsmI"
                     /locus_tag="OO135_001273"
     CDS             complement(1000..1860)
                     /gene="rsmI"
                     /locus_tag="OO135_001273"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5397193.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1924..3960
                     /gene="lpoA"
                     /locus_tag="OO135_001274"
     CDS             1924..3960
                     /gene="lpoA"
                     /locus_tag="OO135_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5397194.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPAQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSHVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3918..4313
                     /locus_tag="OO135_001275"
     CDS             3918..4313
                     /locus_tag="OO135_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5397195.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4333..4923
                     /gene="diaA"
                     /locus_tag="OO135_001276"
     CDS             4333..4923
                     /gene="diaA"
                     /locus_tag="OO135_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5397196.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4933..5508
                     /gene="yraP"
                     /locus_tag="OO135_001277"
     CDS             4933..5508
                     /gene="yraP"
                     /locus_tag="OO135_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5397197.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKHIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSKVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5622..6662)
                     /locus_tag="OO135_001278"
     CDS             complement(5622..6662)
                     /locus_tag="OO135_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5397198.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     QVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQCGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6735..7370)
                     /locus_tag="OO135_001279"
     CDS             complement(6735..7370)
                     /locus_tag="OO135_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5397199.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHILVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7498..8016
                     /gene="yhbO"
                     /locus_tag="OO135_001280"
     CDS             7498..8016
                     /gene="yhbO"
                     /locus_tag="OO135_001280"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5397200.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7996..8439)
                     /locus_tag="OO135_001281"
     CDS             complement(7996..8439)
                     /locus_tag="OO135_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5397201.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8490..8792
                     /gene="yhbQ"
                     /locus_tag="OO135_001282"
     CDS             8490..8792
                     /gene="yhbQ"
                     /locus_tag="OO135_001282"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5397202.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8779..9282)
                     /locus_tag="OO135_001283"
     CDS             complement(8779..9282)
                     /locus_tag="OO135_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5397203.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9276..9800)
                     /locus_tag="OO135_001284"
     CDS             complement(9276..9800)
                     /locus_tag="OO135_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5397204.1"
                     /translation="MLDKLCSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10009..10989
                     /locus_tag="OO135_001285"
     CDS             10009..10989
                     /locus_tag="OO135_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5397205.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10998..11876
                     /locus_tag="OO135_001286"
     CDS             10998..11876
                     /locus_tag="OO135_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5397206.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            12082..13089
                     /locus_tag="OO135_001287"
     CDS             12082..13089
                     /locus_tag="OO135_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR5397207.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13207..14451)
                     /gene="mtr"
                     /locus_tag="OO135_001288"
     CDS             complement(13207..14451)
                     /gene="mtr"
                     /locus_tag="OO135_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5397208.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14605..16494)
                     /gene="deaD"
                     /locus_tag="OO135_001289"
     CDS             complement(14605..16494)
                     /gene="deaD"
                     /locus_tag="OO135_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5397209.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16487..16567)
                     /gene="yrbN"
                     /locus_tag="OO135_001290"
     CDS             complement(16487..16567)
                     /gene="yrbN"
                     /locus_tag="OO135_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5397210.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16674..17558)
                     /gene="nlpI"
                     /locus_tag="OO135_001291"
     CDS             complement(16674..17558)
                     /gene="nlpI"
                     /locus_tag="OO135_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5397211.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17667..19802)
                     /gene="pnp"
                     /locus_tag="OO135_001292"
     CDS             complement(17667..19802)
                     /gene="pnp"
                     /locus_tag="OO135_001292"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5397212.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20049..20318)
                     /gene="rpsO"
                     /locus_tag="OO135_001293"
     CDS             complement(20049..20318)
                     /gene="rpsO"
                     /locus_tag="OO135_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5397213.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20467..21411)
                     /gene="truB"
                     /locus_tag="OO135_001294"
     CDS             complement(20467..21411)
                     /gene="truB"
                     /locus_tag="OO135_001294"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5397214.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMLMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21411..21812)
                     /gene="rbfA"
                     /locus_tag="OO135_001295"
     CDS             complement(21411..21812)
                     /gene="rbfA"
                     /locus_tag="OO135_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5397215.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21976..24648)
                     /gene="infB"
                     /locus_tag="OO135_001296"
     CDS             complement(21976..24648)
                     /gene="infB"
                     /locus_tag="OO135_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5397216.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24673..26160)
                     /gene="nusA"
                     /locus_tag="OO135_001297"
     CDS             complement(24673..26160)
                     /gene="nusA"
                     /locus_tag="OO135_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5397217.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(26188..26640)
                     /gene="rimP"
                     /locus_tag="OO135_001298"
     CDS             complement(26188..26640)
                     /gene="rimP"
                     /locus_tag="OO135_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5397218.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEVVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26847..26923)
                     /locus_tag="OO135_001299"
     tRNA            complement(26847..26923)
                     /locus_tag="OO135_001299"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26887..26889),aa:Met,seq:cat)
     gene            27271..28614
                     /gene="argG"
                     /locus_tag="OO135_001300"
     CDS             27271..28614
                     /gene="argG"
                     /locus_tag="OO135_001300"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5397219.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
BASE COUNT         6828 a         7885 c         7197 g         6726 t
ORIGIN      
        1 ggaaaatcga tactggaaaa actctaactc ggtggccagt ttttgttgac cacttcaaac
       61 tggcaatacg cctgttcctg ctctgcgggt aaggcagtta aacttgtcta tatggtcagc
      121 cccgtactta ccaccactgg acatcagaca ggatattaca aactcaatga cagcctggat
      181 attaaaacca cattacaggc aaacgacatt ccaggactca caaccgacca ggttgtctct
      241 gttaacaccc gattcacaca gataaaaagc agcaccgtat attctgctgc aacccaaacg
      301 ggtgtttgcc agggtgacac gtctcgttat ggacccgtta atattggtgc aaataccacc
      361 tttaccctgt atgtcaccaa gccatttctc ggctcgatga ccattccgaa aacggatatt
      421 gccgtcatta aaggcgcgtg ggtcgatgga atgggaagcc cgtctacagg tgacttccat
      481 gatttagtca agttatcgat tcagggaaat ttcaccgccc cacagtcgtg caaaattaat
      541 cagggcgatg ttattaaggt taattttgga ttcatcaatg gtcagaagtt taccacccgc
      601 aatgccatgc cagacggttt tactccagta gactttgata tcacttatga ctgtggtgat
      661 acttcaaaga ttaaaaactc gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac
      721 cagtacaacc tggtcgccag gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt
      781 attgaaaatc tcggcggcgg agttgcaaat attccttttc agaacggtat ccttcccgtt
      841 gatccttccg ggcatggcac cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt
      901 gagctggaaa caggaaaatt tcagggcaca gccaccatta ccgtcatggt gcggtaaaag
      961 aagacgttgc gttttgatta acgcaacgct tcggcactgt tacccctgct gctccagcgc
     1021 atacttatac agcgcatttt tcttcacgcc atgaatttct gcgaccagcg ccgccgcttt
     1081 tttcagcggc agttctgcct gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc
     1141 ttcttcctgt gctttatgac cttcgacaat cagcaccatt tcgcctttgc gacggttttc
     1201 atcttccttt acccacgcca gcagttcgcc aacgggcgcg ccgtgaatgg tttcccaggt
     1261 tttggtcagc tcacgcgcca gaaccacgta gcgggattcg cctaataccg caacgatatc
     1321 ttccaggcta tctaacagac ggtgggtgga ttcataaaaa atcagcgtgc gcggctccgc
     1381 ttcaatggct tttagcgcat cacggcggcc ttttgatttg gcaggtaaaa agccttcgta
     1441 acagaaacgg tcagagggta aacccgcggc gcttaacgca gtgatagcag cacacggccc
     1501 gggtagcggc accacgcgaa tccccgcttc acggcaggta cgcaccagat ggtagccagg
     1561 atcgttaatt agcggcgttc cggcatcgga aaccagcgca atgttttgcc cctcttgcag
     1621 cttcgccagc agcgtttcgg ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg
     1681 ggcattaatc ccaaaatgtt gcagcaataa accggtgtga cgagtatcct cggcggcaat
     1741 cagatcaacg gcctgtaata cctctaacgc acgctgggtg atatccgcca gattgccgat
     1801 tggtgtcggt acaatgtaaa gctggccctg agaattatcc gccgattggt gttgtttcat
     1861 tgtgtcgtcc gtattgccga tttaatattg agcattgcgt aaaaaaatat cactggatac
     1921 attatggtac cctcaacatt ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg
     1981 gcagccctga ttttcgccgg ttgtggcacc catactcccg ctcagtccac tgcttatatg
     2041 cagggcacgg cgcaggctga ttctgccttt tatcttcagc agatgcagca aagctctgat
     2101 gataccagga tcaactggca attactcgcc attcgtgcac tggtgaaaga aggtaaaacc
     2161 gggcaggcgg ttgagttgtt taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc
     2221 gagaaaacac tgctggcggt agagattaaa ctggcgcaga aagattttgc tggcgcgcaa
     2281 aacttgctgg cgaaaatcac acctgccgat ttagaacaaa accagcaagc gcgttactgg
     2341 caggcaaaaa tcgatgccag ccaggggcgt ccttccattg atttactgcg cgcgttaatt
     2401 gctcaggaac cgctgctcgg cgcgaaagaa aaaaagcaga atattgatgc cacctggcag
     2461 gcgctctcct ccatgactca ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat
     2521 attctgcaag gctggctgga tctgcagcgc gtctggtttg ataaccgtaa cgatcccgat
     2581 atgatgaaag ccgggatcgc cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa
     2641 atgctgccaa cgcagttggt taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc
     2701 gccctgctgt tgccgctgaa tggccaggca gcggtatttg gtcgcactat tcagcaaggc
     2761 tttgaagcgg cgaaaaatat cggcactcag ccagtggcgg ctcaggtagc tgccgcacct
     2821 gccgcagacg tagcagaaca acctcagccg caaactgcgg atggcgttgc cagcccggca
     2881 caagcctcgg ttagcgatct gaccggtgat cagcctgcgg cccagccggt gcctgtaagc
     2941 gccccggcga caagcaccgc agcggtaagc gcacccgcaa atccatccgc agagctgaaa
     3001 atctacgata cctcatcaca accacttagc cagatcttaa gccatgttca gcaggatggc
     3061 gcgagtattg tggttggtcc gttgctgaaa aataacgttg aagagttgct taagagcaac
     3121 accccgctga acgtgctggc actgaaccag ccggagaata tcgaaaaccg cgtcaatatt
     3181 tgttacttcg cgctttcacc ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac
     3241 cagggtaaac aagcgccgct ggtgctgatc ccacgcagtt cattgggcga tcgcgtagcc
     3301 aatgcgtttg cgcaggagtg gcagaaactg ggcggcggca ccgttctgca acaaaaattt
     3361 ggttccacca gcgaattacg cgcgggtgtt aacggcggtt ctggtatcgc tttaacgggt
     3421 accccgatta ctcccagagc gacaaccgac tccggcatga cgaccaacaa tccaacgctg
     3481 caaaccacgc caaccgatga ccagttcacc aataatggcg gtcgtgtcga tgcggtgtac
     3541 attgtggcaa cgccgggtga aatcgctttt atcaaaccga tgatcgccat gcgtaacggt
     3601 agccagagcg gtgcaatgct gtacgccagc tcccgcagcg cacaaggcac cgcaggcccg
     3661 gatttccgtc tggagatgga aggtttgcag tacagcgaaa tcccgatgct ggcgggcggt
     3721 aatctgccgt taatgcagca ggcactcagc gcggtgaata acgattattc actggctcgc
     3781 atgtatgcga tgggcgtcga tgcctggtcg ctggcaaatc atttctcaca aatgcgccag
     3841 gttcagggtt ttgaaatcaa cggtaatacc ggaagcctga cggcaaaccc ggattgcgtg
     3901 attaacagga agttatcatg gctacagtac caacaaggtc aggtagtccc cgccagttaa
     3961 ccaccaaaca gaccggcgat gcgtgggaag tacaagcgcg tcgctggctg gaaggcaaag
     4021 gactgcgatt tgtcgccgct aacgtgaacg agcgtggcgg tgagatcgat ctgataatgc
     4081 gtgaaggctg gaccaccgtt tttgtcgagg tgcgttaccg ccgctctgcg ctttatggcg
     4141 gcgcggcagc cagtgtgacc cgcagcaaac aacacaaatt attacagact gcccgcttgt
     4201 ggctcgcgcg tcataatggg agttttgata ctgtggattg ccggttcgat gtggtagcct
     4261 tcaccgggaa tgaggttgag tggattaagg atgcctttaa tgaccactca taattaaggt
     4321 ttaaggatta gcgtgcaaga aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa
     4381 attgcggcgg cagaggcgct tccggatgcc atctcccgtg cagccatgac gctggttcag
     4441 tctctgctca atggcaacaa aatcctctgt tgtggtaatg gaacttccgc tgccaatgca
     4501 cagcattttg ctgccagcat gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc
     4561 attgcactaa atactgataa tgttgtctta acggcgattg ccaacgatcg cttacatgat
     4621 gaagtgtatg caaaacaggt gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt
     4681 tccacccgtg gcaacagccg cgatattgtt aaagcagttg aagccgccgt tacgcgtgat
     4741 atgaccattg tggcattgac cggctatgac ggcggcgaac ttgcaggttt gttagggcca
     4801 caggatgtgg agatccgcat tccttcgcat cgtagtgctc gcattcagga aatgcatatg
     4861 ctgacggtaa attgcctgtg cgatctgatc gataacacgc ttttccctca ccaggatgat
     4921 taaggagaat acatgaaggc attatcgcca atcgcagtcc ttatttccgc gctgctgttg
     4981 caaggttgtg ttgccgctgc cgtagtgggt accgctgctg tgggtaccaa agccgcaact
     5041 gacccacgca gtgtcggcac ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc
     5101 gcattgtcga aagacgaaca gattaagaaa gaaacccgta ttaatgtcac ggcgtatcag
     5161 ggcaaagtgc tgctggttgg gcagtcacca aatgctgaac tttcggctcg cgccaaacat
     5221 attgctatgg gcgtagacgg tgccaacgaa gtgtataacg agattcgtca gggccagccg
     5281 attggtctgg gcgaagcatc taacgatacg tggatcacca ccaaagtgcg ttcgcagctc
     5341 ttaaccagcg acctggtgaa atcgtccaaa gtgaaagtga ccaccgaaaa cggtgaagtg
     5401 ttcctgatgg ggctggtgac tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg
     5461 gtgagcggcg taaagcgggt aaccacagcg tttacgttta ttaaatagca gccccttgta
     5521 atgcctgatg cgacgcttgc cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg
     5581 gataaggcgt ttacgccgca tccgacatgt tttccctcaa atcagaacaa cagcgccagc
     5641 ccgccgacaa tcacaccaga cactgccacc atcgcccctg tcagccataa ggctttcgcc
     5701 gggaacgctt tgcgcagcat aatcagtgac ggcaaactca ctgccgggag cgtcatcaac
     5761 aatgccagcg ccggagcggt tcccatacct gccagcatca tcgtttgtac aatcggaatt
     5821 tctgctgccg tgggaatgac aaacaagcat cctgctaccg ccatcgccac cacccacatc
     5881 aggctgttat cgacagcacc atcggcatgg gggaataacc agacgcgtgc ggcacccaac
     5941 accagtactg caaggatgta aaccgggatc gtactccaga aaagtgtcca tagcgccctg
     6001 ccccagcggc taaaaaaacc gcactgtgct tccggtatgt caatttcgac cggtgcctgc
     6061 gtttgcggtg tttcacgcac ccatttttgc accagcgtcg caatcagcaa caccatcacc
     6121 agcccggcca ccagacgaat cgccgcaaaa ccccagccga ggacaaaccc cataaacacc
     6181 tgcgtcgccg ggtttaacac cggattgccc atccagaatg ccagcgcacc gcccatcgat
     6241 acctgttggc gacgcatccc cgccgcgacc ggagccgcac agcaggtaca catcatgccc
     6301 ggcagcgaaa acagcgttcc cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc
     6361 aacaaccagt cacgcgggat cagcacctga atcaacgaac cgaggatcac ccccagcacc
     6421 gccgctttcc atactgcgag gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga
     6481 ttagcatccg cctgcgcaag gatagattta ccgatactgt gggtttcggc tgcagtaaac
     6541 gctttgccgt agtaatgttg ccatttcaca taccagagac cggcaatgac aacgagaaag
     6601 aaaagcgcgg gtttccacca ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc
     6661 atagcattcc ctaggaataa gttatgataa ttgagcgcgt gaatattacg ctcactatca
     6721 attcttggga ataattattc agctcttttg cgtaattctg aagagcttaa aatcgtcacg
     6781 ccttcatgct caggccgcat cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca
     6841 atggatttcc agttacctgg taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc
     6901 cgctgtttgc tacgatcgcc cagtaacatc gacgggcgag caatggtcaa tttcggccag
     6961 ttctgggcaa ttaatgcttc ttccatctcc cctttgacgc ggttatagaa aaacggcgag
     7021 tgggcattgg cccccatagc actgaccacc agaatatgct gcgcgcccag ccgccgcccg
     7081 gttaatgcgg tatctactac cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc
     7141 gcttctcgcc gcgtggtgcc gagacaacaa aacacaatgt cgatgggttc ggtgacctgc
     7201 gccaacgcgt cactcagttg cggatcatgg ggattaaaca ccccgggcat atcgcccaac
     7261 ggacgtcgcg tcggcgcagc aatggcgtta actttcggtt cattaatcaa catccgcagc
     7321 aggtgaccgc ccaccagccc cgttgcgcct gtaatcagta cctgactcat cttcgctcct
     7381 ttacagaatt gtctgccttg cgctccacgg ctcatgcacc aggcttaata gaccatgagg
     7441 taattatccc cgattgtggg aaattcgcct catccaatgc aacaacgcgg aggaagcatg
     7501 agtaagaaaa ttgccgtttt aatcagtgat gaatttgagg attcagaatt tacttcaccc
     7561 gcagacgagt tccgtaaagc cggacacgaa gtaattacca ttgaaaagca agcgggtaaa
     7621 acggtgaaag gcaaaaaagg agaagccagc gtgaccatcg ataaatccat cgatgaagtg
     7681 acgccagcgg agtttgatgc cctgctgcta ccgggcggcc attcaccgga ttatctgcgt
     7741 ggggacaacc gttttgtcac ctttacccgt gattttgtga atagcggcaa acctgttttc
     7801 gccatctgtc acggtccgca gctgctgatc agcgccgatg tgattcgggg gcgcaaactg
     7861 accgcagtta aaccgatcat tattgatgtt aaaaatgcgg gcgcggaatt ttacgatcag
     7921 gaagtcgtgg ttgataaaga tcagctggtt accagccgga caccggacga tctgccagcg
     7981 tttaaccgcg aggcgttacg cctgctcggt gcctgagtcg cgtagccaaa tcattttttt
     8041 accaaagccc agcgtgttgt cggtgaattt gatttcatcg agccggattt cccacaccgg
     8101 ggccgacagc attctggcaa ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct
     8161 ttcctcacct tccagcctgc ggatctcacc tttaaactgc acaccgcgaa ttaacgctac
     8221 cgttttcggc tgaccgttta ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc
     8281 gtggcgcgtt ttttcttccg tcaaaatgta gaaggcgact ttctgcgcat caaacagata
     8341 aaaagcatta gcgcaccaca gttccccttc ctgctgcaca caccaggtga caacatgttg
     8401 ttttgccaac caacggctga tggtgatgag tgtttccatt gctgttctct tttatactgt
     8461 gggcctgacc ttaacatgcg acgcagacga tgacaccctg gtttctttac ttgatccgta
     8521 ccgccgacaa taagctttat accgggatca ccacggatgt cgaacgccgc tatcagcagc
     8581 accaaagcgg caaaggggcg aaagcactgc gcgggaaagg agaactgacg ctggcgtttt
     8641 ctgcgccagt gggcgaccgt tcgctggcac tacgggcgga atatcgcgtt aagcaactga
     8701 ccaaacggca aaaagagcgt ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca
     8761 gcctgcaaac cccggagatt aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt
     8821 tacgccattc agcgcgtcat ccgctaaacg atgtacctgg aaggcgcttt cagtgcccgg
     8881 ccagcggcaa cgcagatcgt gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc
     8941 cggatcgccc agcgtcacca ctgcggcata gccgaactca ttaagcgaat cgagtccttc
     9001 atagaccagt tggcgtgcca gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc
     9061 catgccgacc cattgcaggt cttcgccctg cacatcaacc ggactaaatg ccacatagcc
     9121 aatgacctga ccttcgtcat ctgtcgccac cagccccagc gtcagaaagc catcttcacg
     9181 cagatcgtga accagcttcg cttccgcatc actttcgaat gagcgacgca gcagggcatc
     9241 aatacccggc gcatcaatgg gaatttctac tcgaattagc atggttcacc taccgatgtc
     9301 tgtttggttt caggcgcggt tttcattccc gcctcaacaa aatccgccag ttgcagcagc
     9361 atcatgcgta aggctttcgg catttgctcc agttcaatgg cgtccatcag gtttttcaca
     9421 tacagcccca gctccgtatc gccttcaatc accagccgac gctggaagaa gagcgtatct
     9481 ggatcttgtt tacgcgccgc aatcatcagc agatcgctgg cgtcggcact aaaactcaca
     9541 tcagcttgcg cgttctggct gaccaccagt ttgccattca ccaccgaggt aaaccattgc
     9601 aggtcaatat cacgcacatg aatacttaac cagcggcctt caagaaactc cagctcgcca
     9661 tcatccagcg cctgacggaa ttgccagctt aagacctgct caagaacctg gcgttttagc
     9721 gcaaatggcg tcagttttac cggtacactc aacagagatg gccccaaatg cacaatacgg
     9781 gaacacagtt tatccaacac gagttttact ccctgtttca acaatcatcc tattttgcca
     9841 tatcagaaaa ataacatagc ggtataaatc aacaattcca tatgaaattg ctgctaccac
     9901 caatacaact ttaactgcct taaatcaaaa attgtcgcag caaggttaac taaaatccca
     9961 gttcgttaac atttttgcgt tttgatagcg caaccttcag gaaaaattat ggagctgctc
    10021 tgccctgccg gaaatctccc ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt
    10081 tatatcgggc taaaagatga taccaatgcc cgtcacttcg ccggccttaa ctttaccgag
    10141 aaaaaattgc aggaagcggt gagttttgtc catcaacatc gccgcaaact tcacatcgcg
    10201 attaacactt ttgcgcatcc ggacggttac gcccgttggc agcgcgccgt ggatatggcg
    10261 gcgcagctgg gtgccgacgc gctgatcctc gccgacctcg ccatgctgga atacgccgcc
    10321 gagcgttatc cgaatattga gcgccacgta tcggtgcagg cttcggcgac caatgaagag
    10381 gcgattaact tttatcatcg ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg
    10441 tcgattcatc aggtgaaaca gctggcacgg gtcacacctg taccactgga agtgtttgct
    10501 ttcggcagcc tgtgcattat gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt
    10561 gagtcgccta acaccgtggg cgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg
    10621 ccgcaggggc tggaatcccg cctgaacgaa gtgctgatcg accgttatca ggacggcgaa
    10681 aacgcaggtt atccgacgct gtgtaaaggg cgttatctgg tggacggcga gcgctatcac
    10741 gcgctggaag aaccaaccag tctcaatacc ctggaactgc tgccggagtt aatggcggcg
    10801 aatattgctt cggtgaaaat tgaaggccgc cagcgtagcc cggcgtatgt cagccaggtg
    10861 gcgaaagtct ggcgtcaggc tatcgaccgt tgtaaggccg atccgcaaag cgcgtggatg
    10921 gagacgctcg ggtcgatgtc cgaaggcacg cagaccaccc ttggcgcgta tcaccgtaaa
    10981 tggcagtgag aagagcaatg aaatattcct tagggccagt gctgtggtac tggccaaaag
    11041 agacgctgga agatttttat cagcaggccg ccaccagcag cgccgacgtg atttatcttg
    11101 gtgaagcggt atgcagcaag cgccgggcaa ccaaagttgg cgactggctg gagatggcaa
    11161 aatcgctcgc cggaagtggc aagcagattg tgctctccac gctggcgctg gtgcaggcat
    11221 cctctgaact gggcgaactg aaacgctatg ttgagaacgg tgagtttctg attgaagcca
    11281 gcgatctcgg cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc gccgggcacg
    11341 cgctgaactg ctacaacgcg gtgacgctga aaatattgct caaacagggc atgatgcgct
    11401 ggtgtatgcc ggtggagctt tcccgcgact ggctggtgaa tctgcttaat cagtgcgatg
    11461 agctgggcat tcgcaaccag tttgaagtgg aagtcctgag ctacggtcat ctaccgctgg
    11521 cctactccgc tcgctgcttt accgcgcgtt cggaagaccg cccgaaagat gagtgtgaaa
    11581 cctgctgcat taagtatccg aacgggcgca acgtgctgtc gcaggaaaac caacaagtgt
    11641 ttgtactcaa tggcattcag accatgagcg gctacgttta caacctcggt aacgagctga
    11701 catccatgca gggattggtt gatgtggtcc gcctgtcacc gcagggtact gacactttcg
    11761 cgatgctcga cgccttccgc gctaatgaaa atggcgctgc gccactattg ttgacggcaa
    11821 acagcgactg taacggctac tggcggcggc tggcaggact ggagctgcaa gcctaagtaa
    11881 atatgctcgt catacttcaa gttgcatgtg ctgcatctgc attcgttcac cccagtcact
    11941 tacttatgta agctcctggg gattcactct cttgtcgcct tcctgcaact cgaattattt
    12001 agagcacagc tcactttgtt aacaacttta actactcttt aatgcagtat taaagattaa
    12061 tcggtaacaa ggtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg
    12121 gcccccattc ccgaaggttc ttcagcgcga gaagcattct cccactctct cgatctcgcc
    12181 cgtctggctg aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgact
    12241 ggcattgcca gtgctgccac gtcggtattg attggctatc tggcggcgaa taccaccacg
    12301 ctgcatctgg ggtctggcgg cgtgatgttg cctaaccact caccgttggt cattgccgaa
    12361 cagttcggca cgctcaatac actctatccg gggcgaatcg atttggggct gggtcgtgcg
    12421 ccaggtagtg accaacggac aatgatggcg ctgcgtcgcc atatgagcgg cgatattgat
    12481 aatttccccc gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg
    12541 catgtgcgcc cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagt
    12601 ctttacagcg cgcaactggc ggcgcagctt ggtctgccgt tcgcgtttgc ctcacacttc
    12661 gcgccggata tgttgttcca ggcgctgcat ctttatcgca gcaacttcaa accgtcggca
    12721 cgactggaaa aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc
    12781 gatgccgaat tcctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgcggcgaa
    12841 accgggcaac tgccgccacc gattcaaaat atggatcagt tctggtcgcc gtccgaacag
    12901 tatggtgtgc agcaggcgct gagtatgtcg ttggtaggtg ataaagcgaa agtgcgtcat
    12961 ggcttgcagt cgatcctgcg cgaaaccgac gccgatgaga ttatggtcaa cgggcagatt
    13021 ttcgaccacc aggcgcggct gcattcgttt gagctggcga tggatgttaa ggaagagttg
    13081 ttgggatagt gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta
    13141 gacctgataa gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac
    13201 cgctgattac tgatacaccg gcagtaaatt aaagctcgat aaaatatgta ccaatgcgtt
    13261 gccgacgcca aacaccagaa tcagcgtaat catcggcttg ccgccccaga cgcggaattt
    13321 cgggctgcca aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca
    13381 gatggtagcc gctaaaccag cataaccaat ggcgtacagg aatccattcg ggaacagcag
    13441 accacccaca acaggcggtg caaaggtcaa caatgctgtt ttcaagcggc ccatagccga
    13501 gtcatcgaaa ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa
    13561 cgaactcgct accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt
    13621 cagcacgccg cttaatgcct gtaccagcac atcaatatta ccgcccttct ctgcaatacc
    13681 gataaactcc ggacgcggga tgttacccat cgtcgccagc aaccagatgg tatacagcgc
    13741 cagtgccatc agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta
    13801 atacttcatc aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcaa
    13861 tgtcatcagc agatacggcg cataagatgc attgctttct gcaacgttga acaatgtcgc
    13921 aggctgcaca tgccccaaca ggctgccaaa ggtgaggaag aaggtaatga ctttcgcccc
    13981 cagcacaatc gccgtcatgc ggctgacggc cttagtactc aaccacacca caaacgctac
    14041 cagcagtgca aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa
    14101 ggtgtgatgc agaattgaac cactggcaga aatataggca taggtcagga tatagagcac
    14161 aaaggcaatg gaaataccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat
    14221 ggtgtcaaaa ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga
    14281 atgcagcata cagaaccagg taaagatcag cgccgccatt gaccagaaaa accacgcccc
    14341 ggacatgacc actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac
    14401 cacgccgcca agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc
    14461 tctccagtga aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacgcgagt
    14521 acaaaagaca gaaaaaaagc cccgatggta aaaatcgggg ctgtatatat tattttacag
    14581 attgtgttcg ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga
    14641 gtcatcacga cgcggagcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc
    14701 acggccacct tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gttcgccacc
    14761 gaaaccacga ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa
    14821 ctgcatgttc atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc
    14881 cggcatacct ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccaat
    14941 gtaacggctg ctgatgtcgc cttcgttagc aatcgcacca acgatatgac gaacttcaac
    15001 accatcatca cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc
    15061 acgacgcgga cgatcttcac ggtcaccacg cgggccacgg tcgttacgat cgcgcggacc
    15121 acggtcatca cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggtacgat
    15181 cagagtacgt tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag
    15241 ctcttcacct tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc
    15301 gctgctttcc agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag
    15361 cagttctgcg ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt
    15421 gcgcagcaga cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc
    15481 acgaccggta cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt
    15541 aactaccagg ctgatacgct caacgtccag gccacgggct gcaacgtcgg tcgcaatcag
    15601 gatgtccaga cgaccatctt tcaggcgttc cagtgtctgt tcacgcagcg cctggttcat
    15661 gtcaccgttc agcgcggcgc tgttgtagcc gttacgctca agagcttcag ccacttccag
    15721 agtcgcgttt ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa
    15781 acgtaccagt gcttcgtttt tgcgcatacc ccagacagtc cagtagctct ggctgatgtc
    15841 aggacgggta gtcacgctgg actgaatgcg cacttcctgc ggctctttca taaagcggcg
    15901 ggtaatgcga cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc
    15961 cgggatctgc gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc
    16021 agcttcatcc agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg
    16081 gtccagcaga cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa
    16141 ttgcacgtca taacgctggc cgccgtacag agcaaccaca tttacgccgc gcatgtgttt
    16201 agagaaatcc gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac
    16261 cagaatctgt ggtgctttca gctcaggatc aagattctgc aacagaggta aagagaatgc
    16321 tgcagttttt ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg
    16381 tggaatacac tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc
    16441 aaggatagga gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta
    16501 cgtgtgcctc aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa
    16561 ttttcattga aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc
    16621 acccgttaag gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc
    16681 tggtccgatt ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg
    16741 tatcggtgct caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg
    16801 gcgctgtcca aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg
    16861 agatgctcag cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt
    16921 tgttcgctaa tgttgcccaa gtagaactcg acaatgttcc atccccactg ttccttatcc
    16981 gatttttcga agtgctgttt caacacttct ttagcctgct tctcatcgag cttctgctcg
    17041 gcgagataaa gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc
    17101 agatcatctt gcgctaactt atcacgaccg ccgtaatata atgcgatccc gcgattcaag
    17161 tgcgcgtagt tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca
    17221 tcaaaattgc ctgcctgcgt taaatatatg cctaagtaat tgaacacttc aggcatatcc
    17281 ggtcggattg ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta
    17341 tcatacaaca ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga
    17401 ctggcaagga tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat
    17461 ggtaccgcga ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt
    17521 gctgtcgcaa cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg
    17581 ttgaatgaac gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc
    17641 gccgcagcgg agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt
    17701 gctgcagcag gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg
    17761 cgatcaactt ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact
    17821 ttctcaacgc gtttgtcagc gatttgagag atgtggacca gaccttcttt accgccgccg
    17881 atggcaacaa atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc
    17941 acttcgattt ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg
    18001 tcggtcgctg cgatcttcac agtaccgtca tcttcgattt cgatggtagt gccagtttct
    18061 tcggtcaggg cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg
    18121 ttgatcttga tggtatggat acgcggtgcg aactcagaga tatcgccacg cggcgcgttg
    18181 atcgcctgtt ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca
    18241 acctgcatga tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata
    18301 ccctcgcggg aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa
    18361 atgtcagaca gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca
    18421 acggcagctt tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa
    18481 gccatagaag aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg
    18541 aatttgtcca tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca
    18601 atttcacgac gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag
    18661 ttgtagtgga acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg
    18721 tcacgagcag tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc
    18781 gcagaaccgt gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct
    18841 ttttcacgac cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc
    18901 tcgatagcgt gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc
    18961 agcgtcgcga tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg
    19021 gtgatgcggt aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc
    19081 gcttcgttta ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt
    19141 tcattgatgt tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc
    19201 atctggtctt cgctcagcag ttcagcttca gattcaacca tcagaacagc ggcttcagta
    19261 ccggcaacaa ccagatccag tttgctctct ttcagctcgt cctgagtcgg gttcagtacg
    19321 tactggtcat tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca
    19381 gacagagaca gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg
    19441 ttaacagaaa ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc
    19501 gggcgaatcg ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tggacgacct
    19561 tcacgacgga agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta
    19621 acggtcagtg ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca
    19681 aataccgcgg tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg
    19741 ccggtttcca gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc
    19801 aatgtaatat cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat
    19861 cttctgcgca tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc
    19921 tcattagccg cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc
    19981 cagtgaattg ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact
    20041 cgcaggaatt agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct
    20101 ttacgtttca ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga
    20161 cggctgtggt gatctttttt gtgctctgca aagtgcccct gcaggtggtt gatctgtgca
    20221 gtcagcagtg ctacctgaac ttcggtagaa ccggtgtcgt ttgcgtcacg accaaactca
    20281 gaaacgattt tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag
    20341 aatgaaagga cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa
    20401 caatttacgc gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg
    20461 ttaacattac gccgggtatt caaccaccag gcgacgaggc gcgacgcggc cttcatcgtc
    20521 aatttcgccc ataccgataa acttgccgtt ctcaccttct gtaacgcgaa ccagtccttc
    20581 cagcggcgca ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa
    20641 cggaagattc accaccgggt agtccgaagc tggactgtcc attagcatca gtaatggatc
    20701 aagtaactcc gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg
    20761 ttccagggtc accatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat
    20821 aacatgcgcg ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt
    20881 gccttttgag cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa
    20941 ttcataaacg gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc
    21001 gtacagtttt ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc
    21061 gccacggaaa gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc
    21121 ttcaacgatt tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac
    21181 ccgatagcgt ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaagca
    21241 aattggcaac atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc
    21301 gttataaatc cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc
    21361 cagcaacaaa acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc
    21421 ttgctgtcgt ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg
    21481 tttgacatgc gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg
    21541 atacgcaggc gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac
    21601 gctttgatgc ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact
    21661 ttggcatacg ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg
    21721 cgaggatctt taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct
    21781 acgcgctgcg ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct
    21841 gttagcccct ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga
    21901 cgcgccagcg tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat
    21961 ggaaccctaa aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat
    22021 cacatcgcca gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt
    22081 acggacttcg ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat
    22141 aaccacgttg tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac
    22201 catacagcct gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag
    22261 accgataatc tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc
    22321 gtcaatcagg ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac
    22381 tttacgtgca gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc
    22441 agccagggtg gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt
    22501 aacttcgtca gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac
    22561 gtctgccttc aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat
    22621 gttctcgagt ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg
    22681 atacagtgca acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc
    22741 agccggtacg ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac
    22801 ttcctgacct agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac
    22861 gatatcgccc ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc
    22921 tttatcgagg aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag
    22981 ctccagaact tccgcctgca gcaggatagc gtccagcagt tcatcgatgc cggtacccgc
    23041 tttcgcagat acgtgtacga actggctttc accgccccac tcttctggca ggatgccata
    23101 ctgggagagt tcgttcttaa cacgatccgg atcggcttct ggtttatcaa tcttgttcac
    23161 tgcaaccacc accggtacct gcgccgcttt cgcgtgctgg attgcttcga tggtctgcgg
    23221 catcacgccg tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg
    23281 agcacgcatt gaagtaaacg cggcgtgccc tggggtgtcc aggaaggtga tcatgccgtt
    23341 ttcagtttca acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc
    23401 cactttcgtt gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat
    23461 gatggtcaca accggcgcgc gcggttcagc cgcagccccc gtgtcacggt cgctcattac
    23521 cgcctcttcc agctcgtttt cacgacgcag gataactttg tggcccatct cttcagcaac
    23581 cagctgtgcg gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat
    23641 catcgctttg atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt
    23701 gatggtttcg ccgatcacaa cgtcacggtt aacggcctga gctggcttct ggaagccttg
    23761 ctgcagggaa gaacctttac gttttccgcc tttacctcca cgtactgctg cgcgagcttc
    23821 ttcacgatca gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc
    23881 gttacgacca cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg
    23941 agcatgttga gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc
    24001 agtccatttg ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc
    24061 ttcgagttta cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc
    24121 acggcgggct ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt
    24181 gtctttttcc gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc
    24241 ggcctcacgt tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg
    24301 ggcttgctct tccgcttcac gctgcgcttg ctcttccgct gcaaggcgtt cagcctcttg
    24361 cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg atttgtaccg atttgctttt
    24421 tccaccggta ccaggaatgt taagggtgct gcgtgtttta cgttgcagcg tcaatttgtc
    24481 cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc tgtttctctt gtgcggacac
    24541 agagtcgtca gcagacttcc ggatacctgc atcagcaaat tgctgtacca ggcgttccac
    24601 ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca tctgtcatgc tgttccttcc
    24661 tgctacagtt tattacgctt cgtcaccgaa ccagcaaata ttacgggcag ccataatcag
    24721 tgctccggct ttttcgtcgg tcaccccttc gatatcagcc agatcatcaa tgccctgttc
    24781 ggcgagatct tccagcgtac aaacgccacg ggcggccagt ttgaatgcca aatcacgatc
    24841 tatcccttca aggttcagca gatcgtcagc cggtttgtta tcaccgaggc tttcttcctg
    24901 ggcctgtgca atggtggcca gtgcattttt agcacgctcg cgcagtgctt caacggtcgg
    24961 ctcatcaagg ccttcgattt ccaacagctc tttcatcggc acataggcca gttcttccag
    25021 cgtcgagaag ccttcttcta ccagaacagt cgcgaagtct tcgtcgatgt cgagatattt
    25081 ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc ttagcctgca ggtcgtcaac
    25141 ggtcatcacg ttgagttccc aaccgctcag ctgcgaagcc agacgcacgt tctgaccgtt
    25201 acggccaatc gcctgcgcca ggttaccggc ttcaacggcg atatccatgg tgtgtttatc
    25261 ttcatccacc acgatagaag caacgtctgc cggtgccatt gcgttaatca caaactgcgc
    25321 cgggttatca tcccacagga cgatatcgat acgctcgccg cccagttcag tagaaaccgc
    25381 ctgaacacgc gcgccacgca tacctacaca ggcacctacc ggatcgatac gtttatcgtt
    25441 ggttttcacc gcgattttcg cacgagaacc cggatcgcga gccgctgctt taatttcaat
    25501 cacttcttcg ccgatttctg gcacttcaat acggaacagt tcgatcagca tttccggctt
    25561 ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg cgaacggaat agagcacgcc
    25621 acgaacgcgg tcgccagggc ggaagttttc acgcggcagc atatcttcgc gcaggatcac
    25681 ggcttcagca ttgttgccca gctccagaga gatgttgtcg cggtttactt ttttcaccac
    25741 gccggtgatg atttcacctt cgtgttcacg gaactgatca accaccatcg cacgttcggc
    25801 ttcacgcact ttctgcacga taacctgttt tgccgtctgg gtagtgatac ggtcaaaggt
    25861 aacagactca atctgatctt caacgtaatc gcccaggttc aggctttcat cttcataacg
    25921 tgcggcttca agggtgattt ccttggtcgg ctgggtgact tcatcaacaa ctaaccagcg
    25981 acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt acgcggacgt cgatctcttg
    26041 ttcatatttt ttctttgttg ctgtcgccag cgcgctttcc aatgcttcga aaatcttctc
    26101 gcgaggtagc gccttttcat tggatacggc ttcaactaca gccaaaattt ctttgttcat
    26161 cgcgggcttt tcacctcatc cagactatta aaagtgggga accaggttcg ccttctggat
    26221 attactcagc gcgaacactt catctttacc ttcgacggta actgtgatca tttcaccgtc
    26281 taccgctttg ataacgccct gccatttacg acggttttgt accgccatac ggagaaccag
    26341 agtcaccact tctccgacaa aacgggcgta gtgttcagcc gtgaacagtg ggcgatcgag
    26401 acccggtgag gagacttcca ggttataagc aacggtgatg ggatcttcaa catccagcac
    26461 agcacttacc tggtggctca catcagcaca atcatcaaca ttgatgccat cttcactatc
    26521 aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat tcgatgccaa ccagttcaaa
    26581 acccagggcc tcaactggcg cagtaatcat ctctgttaat ttttgctcta atgtggacaa
    26641 gcccaccccc aagacataaa aaaagggcct aaagcccagt tattctgtag tcagataaca
    26701 aaaaaccccg ataaatcggg gctttatata actgaaccct ataaccgcaa ctgcggtctg
    26761 gagcactttc cagaaggatt ttttcaaatc ccactacgaa ggccgaagtc ttcacagtat
    26821 atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc ggatttgaac cgacgacctt
    26881 cgggttatga gcccgacgag ctaccaggct gctccacccc gcgcctgaaa cgcggcaaat
    26941 tctactcgtt ttgggtaaaa gatgcaaata ctgctgggat ttggtgtacc gagacgggac
    27001 gtaaaatctg caggcattat agtgatccat gccacatttt gtcaacgttt attgctaatc
    27061 acgtgaatga atatccagtt cactttcatt tgttgaatac ttttgccttc tcctgctctc
    27121 ccttaagcgc attattttac aaaaaacaca ctaaactctt cctgtctccg ataaaagatg
    27181 attaaatgaa aactcattta ttttgcataa aaattcagtg agagcggaaa tccaggctca
    27241 tcatcagtta attaagcagg gtgttatttt atgacgacga ttctcaagca tctcccggta
    27301 ggtcaacgta ttggtatcgc tttttctggc ggtctggaca ccagtgccgc actgctgtgg
    27361 atgcgacaaa agggagcggt tccttatgca tatactgcaa acctaggcca gccagacgaa
    27421 gaggattatg atgcgatccc tcgtcgtgcc atggaatacg gcgcggagaa cgcacgtctg
    27481 atcgactgcc gcaaacaact ggtggccgaa ggtattgccg ctattcagtg tggcgcattt
    27541 cataacacca ccggcggcct gacctatttc aacacgacgc cgctgggccg cgccgtgact
    27601 ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga atatctgggg tgacggtagc
    27661 acctataaag gaaacgatat cgaacgtttc tatcgttatg gtctgctgac caatgctgaa
    27721 ctgcagattt acaaaccgtg gcttgatact gactttattg atgaactggg cggccgtcat
    27781 gagatgtctg aatttatgat tgcctgcggt ttcgactaca aaatgtctgt cgaaaaagcc
    27841 tactccacag actccaacat gcttggtgca acgcatgaag cgaaggatct ggaatacctc
    27901 aactccagcg tcaaaatcgt caacccgatt atgggcgtga aattctggga tgagagcgtg
    27961 aagatcccgg cagaagaagt cacagtacgc tttgagcaag gtcatccggt ggcgctgaac
    28021 ggtaaaacct ttagcaacga cgtagaaatg atgctggaag ctaaccgcat cggcggtcgt
    28081 cacggcctgg gcatgagcga ccagattgag aaccgtatca tcgaagcgaa aagccgtggt
    28141 atttacgaag ctccggggat ggcactgctg cacattgcgt atgaacgtct gttgaccggt
    28201 attcacaacg aagacaccat tgagcagtat cacgcgcatg gtcgtcagtt gggccgtcta
    28261 ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc tgcgtgactc tctgcaacgc
    28321 tgggttgcca gccagatcac tggtgaagtt accctggagc tgcgccgtgg gaacgattat
    28381 tcaatcctga ataccgtctc agagaacctg acctacaagc cagagcgtct gacgatggaa
    28441 aaaggcgact cggtgttctc gccagatgat cgtattggtc aattgaccat gcgtaatctg
    28501 gatatcactg atacccgaga gaaacttttt ggttatgcca aaactggcct gctctcctcc
    28561 tctgccactt caggcgtgcc gcaggtggag aatatggaaa acaaaggcca gtaattcgac
    28621 atcaaccctg cccttc
//
LOCUS       DAJTMK010000035        28500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTMK010000035 DAJTMK010000000
VERSION     DAJTMK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28500
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.036"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..858
                     /gene="ag43"
                     /locus_tag="OO135_001301"
     CDS             <1..858
                     /gene="ag43"
                     /locus_tag="OO135_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5397220.1"
                     /translation="HDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLHYTWQGLTLDDGQDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTKHRVSELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYN
                     GQATLNVTF"
     gene            929..1087
                     /locus_tag="OO135_001302"
     CDS             929..1087
                     /locus_tag="OO135_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5397221.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            1242..2060
                     /locus_tag="OO135_001303"
     CDS             1242..2060
                     /locus_tag="OO135_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5397222.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            2402..2875
                     /locus_tag="OO135_001304"
     CDS             2402..2875
                     /locus_tag="OO135_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5397223.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2891..3367
                     /locus_tag="OO135_001305"
     CDS             2891..3367
                     /locus_tag="OO135_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5397224.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3610..5151
                     /gene="istA"
                     /locus_tag="OO135_001306"
     CDS             3610..5151
                     /gene="istA"
                     /locus_tag="OO135_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCR5397225.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRSHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            5166..5912
                     /gene="istB"
                     /locus_tag="OO135_001307"
     CDS             5166..5912
                     /gene="istB"
                     /locus_tag="OO135_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR5397226.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            6016..6237
                     /locus_tag="OO135_001308"
     CDS             6016..6237
                     /locus_tag="OO135_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5397227.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            6400..6774
                     /locus_tag="OO135_001309"
     CDS             6400..6774
                     /locus_tag="OO135_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5397228.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            6821..7195
                     /locus_tag="OO135_001310"
     CDS             6821..7195
                     /locus_tag="OO135_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5397229.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            7192..7683
                     /locus_tag="OO135_001311"
     CDS             7192..7683
                     /locus_tag="OO135_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397230.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            7695..7892
                     /locus_tag="OO135_001312"
     CDS             7695..7892
                     /locus_tag="OO135_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397231.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            7977..8537
                     /locus_tag="OO135_001313"
                     /pseudo
     CDS             7977..8537
                     /locus_tag="OO135_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            8609..9613
                     /locus_tag="OO135_001314"
     CDS             8609..9613
                     /locus_tag="OO135_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5397232.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            9897..10175
                     /locus_tag="OO135_001315"
                     /pseudo
     CDS             9897..10175
                     /locus_tag="OO135_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(10325..10412)
                     /locus_tag="OO135_001316"
     tRNA            complement(10325..10412)
                     /locus_tag="OO135_001316"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10376..10378),aa:Ser,seq:gga)
     gene            complement(10666..10884)
                     /gene="infA"
                     /locus_tag="OO135_001317"
     CDS             complement(10666..10884)
                     /gene="infA"
                     /locus_tag="OO135_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5397233.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11169..11873)
                     /gene="aat"
                     /locus_tag="OO135_001318"
     CDS             complement(11169..11873)
                     /gene="aat"
                     /locus_tag="OO135_001318"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5397234.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11915..13636)
                     /gene="cydC"
                     /locus_tag="OO135_001319"
     CDS             complement(11915..13636)
                     /gene="cydC"
                     /locus_tag="OO135_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5397235.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13637..15403)
                     /gene="cydD"
                     /locus_tag="OO135_001320"
     CDS             complement(13637..15403)
                     /gene="cydD"
                     /locus_tag="OO135_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5397236.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15526..16491)
                     /gene="trxB"
                     /locus_tag="OO135_001321"
     CDS             complement(15526..16491)
                     /gene="trxB"
                     /locus_tag="OO135_001321"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5397237.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17036..17530
                     /gene="lrp"
                     /locus_tag="OO135_001322"
     CDS             17036..17530
                     /gene="lrp"
                     /locus_tag="OO135_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5397238.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVSDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17665..21693
                     /locus_tag="OO135_001323"
     CDS             17665..21693
                     /locus_tag="OO135_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5397239.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QAQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQQQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21848..22459
                     /gene="lolA"
                     /locus_tag="OO135_001324"
     CDS             21848..22459
                     /gene="lolA"
                     /locus_tag="OO135_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5397240.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22470..23813
                     /gene="rarA"
                     /locus_tag="OO135_001325"
                     /gene_synonym="mgsA"
     CDS             22470..23813
                     /gene="rarA"
                     /locus_tag="OO135_001325"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5397241.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAISELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23904..25196
                     /gene="serS"
                     /locus_tag="OO135_001326"
     CDS             23904..25196
                     /gene="serS"
                     /locus_tag="OO135_001326"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5397242.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25434..27878
                     /gene="dmsA"
                     /locus_tag="OO135_001327"
     CDS             25434..27878
                     /gene="dmsA"
                     /locus_tag="OO135_001327"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR5397243.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGSGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGDVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27889..>28500
                     /gene="dmsB"
                     /locus_tag="OO135_001328"
     CDS             27889..>28500
                     /gene="dmsB"
                     /locus_tag="OO135_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5397244.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKE"
BASE COUNT         7250 a         7279 c         7417 g         6554 t
ORIGIN      
        1 cacgataaca acggcggtat tgcccgtgga gccacgccgg aaagcagcgg cagctatggc
       61 ttcgtccgtc tggagggtga cctgctgaga acagaggttg ccggtatgtc tctgacgaca
      121 ggggtgtatg gtgccgcagg ccattcttcc gttgatgtta aggatgatga cggttcccgc
      181 gccggcacgg tccgggatga tgccggcagc ctgggcggat acctgaatct gacacacacg
      241 tcctccggcc tgtgggctga cattgtggca cagggaaccc gccacagcat gaaagcgtca
      301 tcggacaata acgacttccg tgcccggggc tggggctggc tgggctcact ggaaaccggt
      361 ctgcccttca gtatcactga caacctgatg ctggagccac aactgcacta tacctggcag
      421 ggacttaccc tggatgacgg ccaggataac gccggttatg tgaagttcgg gcatggcagt
      481 gcacaacatg tgcgtgccgg tttccgtctg ggcagccaca acgatatgag ctttggtgaa
      541 ggcacctcat cccgtgacac tctgcgcgac agtacaaaac accgtgtgag tgaactgccg
      601 gtgaactggt gggtacagcc ttctgttatc cgcactttca gctcccgggg tgacatgagc
      661 atggggacag ccgccgccgg cagtaacatg acgttctcac cgtcccggaa tggcacgtca
      721 ctggacctgc aggccggact ggaagcccgt gtccgggaaa atatcaccct gggcgttcag
      781 gccggttatg cccacagcgt cagcggcagc agcgctgaag gttataacgg tcaggccacg
      841 ctgaatgtga ctttttgata atcaggtcac tctccttcag gccccggtca tcatgaccgg
      901 ggctttttac tttcagggag caacagatat gccgggttgt acatcacgtt tattgcccga
      961 aggccctttc tcacgcgaac aggctgttgc cgtcaaaaca gcttaccgca atgtgtttac
     1021 tgaagatgac cagggaacat attcccggct ggttatccgt aatgctgaag gtcagttgcg
     1081 ctggtgatgc cggaacacag cttaatactc agctcgccag ttaagggctt ctcagacaat
     1141 aaacgtcatt catttcatct gtcaggccgc gtcttctccg ggagacgcag ctttttcatt
     1201 tattacacca gtaaatcgta accaccgata aggagcaaat tatgcgacta gccagtcgtt
     1261 ttggttatgc gaaccagata cgccgtgacc gtccgctgac acacgaagaa ctgatgcact
     1321 atgttcccag tattttcggt gaagaccggc atacttcccg cagtaaacga tatgcgtaca
     1381 ttcccaccat caccgtactg gaaagcctgc agcaggaagg ttttcagcca ttcttcgcct
     1441 gccagacccg cgtgcgcgac ccgggccgcc ggggatacac aaaacacatg ctgcgtctgc
     1501 ggcgggccgg agagataaac ggagaacatg tccctgaaat tattctgctc aactctcatg
     1561 acggtacctc cagctaccag atgctgccgg gttacttcag attcgtctgc cagaatggct
     1621 gcgtctgcgg tcagtctctg ggggaagtgc gtgttccaca ccggggaaat gtggtggaga
     1681 aagttatcga aggggcttac gaagtggtgg gggtttttga ccggattgag gaaaagcgtg
     1741 atgccatgca gtcgctggtc ctgccgccac cggcacgcca ggcgctggca caggcggcac
     1801 tgacttaccg ttatggtgac gaacatcagc cagtcaccac cgctgacatt ctgacaccac
     1861 gacgcaggga ggattacggt aaagacctgt ggagcgcata tcagaccatc caggagaata
     1921 tgctgaaagg cgggatttcc ggccgcagtg caaaaggaaa acgtatccac acccgtgcca
     1981 ttcacaacat cgacaccgat attaagctca accgcgcatt gtgggtaatg gcagaaacgc
     2041 tgctggagag cctgcgctga tgcagcatgt ccctgaaggc gtgattcagg caccagaatc
     2101 acccgacgcc ccggaggcgc tgccggggtg tcgttccatt tctgatgatg acctgtgcgc
     2161 ctggtgtacc cgtctgcttt accgtcccgg agaaatcagt ctgtgcagac tgagtataca
     2221 ggcaatggca tctggccatc cgtctgtgac cggaacggtt acgcgtacgg ctgtccggaa
     2281 tttcagccga atatcattca tccctgatat taccgggtca tcacggtcag tgatgaacgt
     2341 gatgttgcca gctcccgacc tttactccct ctcatttatt cacattacaa ggatttcata
     2401 catgaaaaca ttgtctcaga acaccaccgc ttcggcctgt gcaccggaga ctggcctgca
     2461 acaactggtt gccactcccg tccctgatga acagcgcatc agcttctggc cgcagcattt
     2521 tggcctcatt ccacagtggg tcaccctgga accccgtgtc ttcggctgga tggaccgtct
     2581 gtgtgaagac tactgcggtg gtatctggaa tctgtacacc ctgaacaacg gcggggcatt
     2641 tatggcaccc gaaccggatg acgatgatga cgaaacatgg gtactgttca atgccatgaa
     2701 cggtaaccgc gctgaaatga gcccggaagc cgccggcatt gctgcctgtc tgatgacgta
     2761 cagtcatcat gcctgtcgta cggagtgtta tgccatgacg gtccattatt accggctgcg
     2821 ggattacgcc ctgcagcatc cggaatgcag cgccattatg cgcatcatcg actgaatgga
     2881 ggcagggaca atgcagcagc tttcctttct gcccggaaac atgacgcccg gcgagcgcag
     2941 tctcattctg cgggccctgc aaaccctgga ccgccatctt catgaacccg gcgtggcctt
     3001 cacctccacc cgtgcggcac gggaatggct gattctgaac atggcgggac tggagcggga
     3061 agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
     3121 caccggcacc atcaaccgta cggaagttca tccccgggaa gtgattaaac gcgccctgta
     3181 ccacaatgcc gctgccgtgg tgctggcgca caatcacccg tccggtgaag tcacacccag
     3241 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
     3301 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
     3361 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcatgcg
     3421 tattttcatg aaaggagatc actcaataac ttccatcgag atcgggtaat aacatttgaa
     3481 cagatcgctg aataacatcg atggagatca cttttgactc attttgttat tcagtgatct
     3541 ccatcaatgt tattggaact tcacaggtgt gttgatctgt atcttttgcc attccggtaa
     3601 aggataccta tgccaacagt tccaatttct atgagaaaac ttaaagaaat tcttagactt
     3661 aaatacggtg ttggactcag ccatcgacaa attggtcgta gtcttgcaat ctccccttcc
     3721 gttgtatcca gatatgctaa tcgggcggct caacttggca taaagcagtg gcccttacct
     3781 acaggatggg atgatacaaa actaaaacat gcgttccttc agacccaggt taagatgaag
     3841 aagcactctc tgcctgactg ggctacagta caccgggaac tgcgtaataa atgcgtgacg
     3901 ctgcagctac tctgggaaga atactgtgag cgtaatccag gcggttttta cagctataac
     3961 cattactgcc ggatgtaccg tgaatggctc aaaaccactt caccatcaat gcgtcaggta
     4021 cataaagctg gcgaaaaact tttcgttgat tactgtggac ctaccgttgg cgttaccgac
     4081 cctgagaccg gagaaataag aactgctcag gtcatcgtag ctgttctcgg ggcatcaagt
     4141 tacacatggg cagaggccac ctggtctcag cagcttgaag actgggtgat gagtcatgtt
     4201 cgctgcttcc agtggttggg tggcgttcct gaacttgttg ttccggacaa tctgaaaagc
     4261 gccacatcca gggcatgtaa gtatgatcct gacgttaacc ctacctacca gcagatgctt
     4321 gagcattata atgtcgcagt tttgcctgcg cggccacgta aaccgaaaga taaagccaaa
     4381 gctgaagttg gcgttcaggt tgttgaacgc tggatcatgg cccgaatcag gcatgagatc
     4441 ttctacagcc ttgcatcgct taatcagcgc attcgggagt tgctggaaag actgaataac
     4501 aaaataatgc agaagttggg ttattcacgt gcagaactct tcatccagct tgataaaccc
     4561 gcactgaagc ctcttcctga agccagttac agttacaccc tggtgaagaa agtcagagtt
     4621 catgccgatt accacgtgga aatcgacaaa cattactact cggttccatg ttcgctgtta
     4681 ggccagcaac tggaagcatg gatctccgga gaactggtaa gactcttcaa tcaggggcag
     4741 gaggttgctg tgcacccgcg caagcgtact tatggctaca gtacccgcaa cgagcacatg
     4801 cctgaagctc atcgacagca tgccacctgg acgccagagc gtcttctgga atgggcgggg
     4861 cacataggca gtgaaactca tagttatgtg cttcatatac tgaactctcg ttcacatccg
     4921 gaacaaagct atcgcttctg ccttggactc ctgaaccttc ataaaaaata cagtaaagcc
     4981 agacttaatg cagcatgtgc aagagctctg aaaacaaagg tatggcgtct gtcaggtatt
     5041 aaatcgatcc tggaaaaagg tctggataaa caacctgttc aggatccaaa accagatctg
     5101 ttatccacga tggaacacga aaacgtacgc ggcagtgagt attaccactg atacgggatc
     5161 caatgatgaa tcatctttac gaacaactga ccgcacttaa actcaccggc ttccgtgatg
     5221 cgcttaaaaa gcaacttgct cagccgggca cataccagga gctgggcttc gaagaacgcc
     5281 tgtcattact gacagcagaa gaactaacct gccgtgaaaa caggaaggca gagcgtctga
     5341 tcaaacatgc acggttcaga cttaatgctg agttatcaaa gctggattat cgtaacaata
     5401 gagggctgga cagggccctc atccgttcac tcagtcaggg aaactggtta accctgaaac
     5461 aaaatatttt actgaccggg gccaccggca gcggtaaaac gttcctggca tgtgcacttg
     5521 gtcataatgc ctgccgacag ggatacaagg tctactatta tcgccttaaa gcgctgatgg
     5581 aacagtgcta tcaggggcat gctgatggaa gatacagcaa acttttgacc aggctgaata
     5641 atagcgatct gctgcttctg gatgactggg ggctggaacc tctctcatca gaacagcgta
     5701 gcgacctgct ggaaatagtg gatctgatgt accaacgagg ctcaatcatc gtagtgagcc
     5761 agttgccggt ggaaaactgg tacaaaatga tcggagactc cacacatgcg gatgccatcc
     5821 tagatcgact ggttcatggc agtatcaaga tcgaacttaa aggagaatca atgcggaaaa
     5881 tacaatctcc gttgaccgaa ggagatcagt gaaggtaatt taaaaacggt tctgtgaaag
     5941 tgacacgaac cgatctccat cgatgttact caccgatctc cttcacggta atacgcactt
     6001 cagaggtatc acatcatgag aatcatcacc cgtggtgaag ccatgcgtat tcaccaacag
     6061 catcctgcat cccgtctttt tccgttctgt accggtaagt atcgctggca cggcagtgct
     6121 gaagcgtata ctggtcgtga agtgcaggat attcccggcg ttcttgccgt gtttgcagaa
     6181 cgccgtaagg acagttttgg cccgtatgtc cggctgatga gtgtcaccct gaactgatgt
     6241 tccgtcaccc gggaaacgag tacgaccaga tgacctcaga cgccgctgtg gatgaggcca
     6301 ttctcatcaa tgcatacatc ttcaccgaaa ccggtcagcg cgccggctga cctgtcatcg
     6361 ttaaaccatt cttaaccact tttcagagag gattttatcg tgtcagacaa actctccggg
     6421 ataactcatc ccgatgacaa tcacgaccgc ccctggtggg ggcttccctg cacagtgagg
     6481 ccctgttttg gcgctcgtct ggtgcaagag ggtaaccgcc tgtattacct tgccgaccgc
     6541 gccggtatca gaggccgctt cagcgacgca gatgcatacc atctggacca ggcctttccg
     6601 ctgctgatga aacaactgga actcatgctc accagcggtg aactgaatcc ccgatatcag
     6661 cataccgtca cgctgtatgc aaaagggctg acctgcgaag ccgacaccct cggctcctgt
     6721 ggttacgttt atctggctgt ttatccgaca ccagcacccg caaccacctc ataaccccga
     6781 agctctcacc tttagcacta ccagttacag agaacttaag atgaaaacat tatccgacac
     6841 acatgtacgg gaggtatctc gctgcccgtc tcccgtcacc atctggcaga cactgctcat
     6901 ccgactgctg gaccagcatt atggcctcac actgaatgac acaccgtttg tcgatgaacg
     6961 tgtgattgag cagcatattg aggcaggcat ttcactgtgt gatgcagtta attttctcgt
     7021 ggaaaaatac gcgctggtgc gtaccgacca gccgggattc agcacctgtc cccgctctca
     7081 gttaataaac agtattgata tcctccgggc tcgccgggcg accggcctga tgacccgcga
     7141 caattacaga acggtaaata acattaccct gggtaagtat ccggaggcga aatgatgaaa
     7201 ctggccctga cactggaagc cgacagcgtt aacgtacagg cactgaacat agggcgcatt
     7261 gtcgttgacg tcgatggtat tgagctcgct gaactgatta acatggtctg cgataacggc
     7321 tactcccttc gtgttgttga tgaatctgac cagacctcag cagaatgcac gccaccattt
     7381 gctaccctta ccggcatacg ctgcagtacc gcacatatca cggaaacgga caacgcctgg
     7441 ttgtactcgc tgtcacacca gaccagtgac ttcggtgaat cagaatggat tcattttaca
     7501 ggtagcggat atctgttacg taccgatgcg tggtcatacc cggttctgcg gcttaaacgc
     7561 ctggggctgt caaaaacgtt ccgtcgtctg gtcgtcacac tcatccggcg ttatggcgtc
     7621 agtctcattc atctggatgc cagcgctgaa tgcctgccgg gtttacccac tttcgactgg
     7681 taaccaggaa caacatgaaa tcattaacca cggaaacagc gctggatatt ctgattgcgt
     7741 ggctgcagga caatatcgac tgcggatcgg gaattatctt tgacaacgat gaggataaaa
     7801 cggattcagc agcactgttg ccctgtatcg aacaggcccg ggaggatgtc cgtaccctgc
     7861 gtcatctgca gcttctgcac cagaaccggt gaagctcact caccagactt cattcctctg
     7921 acgccagcct gcacagggct ggcgttttca tttatctgca aaaaggaata tcgattatgt
     7981 cagatatcac aatctcccgt ccggaagtgg tcaacgggca tacggacgtt atctgctcaa
     8041 ccagcattcg ccacattctg gctgtacgaa agagtacact gttgcaaatc gacacactta
     8101 tccgacaact ggctgaaatc tcggtgctga cggaaagcat cggaggtaaa actgccccgg
     8161 actgggccat gaaacaggat tttcgctgcg gctgctggct gatggagaaa cccgaaacag
     8221 caatgaaagc cattacccgg aatcttgatc gcgaaatctg gcgtgacctg atgcaacgtt
     8281 ccgggatgct gtcattaatg gatacgcagg cccgtgatac atggtaccgg tcactggagt
     8341 acgataattt tccggaaatc agcgaagcga acattctgag cacatttgaa caactgcacc
     8401 agaataagga tgaggtgttt gagcgagggg tgatcaacgt cttcagaggg ctgaactgga
     8461 attacaaaac caattgcccc tgtaaatttg gcagtaaaat tatcgtcaac aatctggtga
     8521 gatgggaccg gtggggataa tgatatggtc acccccacct taacagcagt acgctgttaa
     8581 ggtaaatccc tttaacggag agaccatcat gcagaacatc acacttattg gtatcgatct
     8641 cggaaagcac tccttccaca ttcattgtca agacaagtct ggtaaggctc ttctgcgtaa
     8701 aaaatttacc cgtactaaac tgatgcagtt tctggcgaca tgtccgccct cagttgttgt
     8761 aatggaagcc tgtgcgggtg cacactttat ggctcgctgt atctctgatt ttgggcatga
     8821 aactaagctg atttctccac agttcgttcg cccgtttgtc aaaagtaata agaatgattt
     8881 tgttgatgca gaagccatat gcgaagcggc ttcacgacca tcaatgcgtt ttgtccagcc
     8941 aagaactgag acccagcagg ccatgagggc gttacaccgg gtcagagaat cgctcatcag
     9001 agataaggtg aaaacaacca accagatcca cggtttcttg ctggaatttg gaatcagcct
     9061 gccgataggt gatgctgtaa taaaacggtt gtctctcgtg ttggctgaac acgaaatccc
     9121 cgaatatctg agccgccttt tggtgaggtt gcatacccac tacttttacc ttgttgagca
     9181 aatagcagag ctggaatcag aattaagtca gtcaatcaac gctgatgata ccgctcagcg
     9241 cattatgaca attcctggtg ttggtccgat tactgccagc ttactatcgt cgcaacttgg
     9301 tgacgggaaa cagttctcat gcagcaggga ttttgcagct tccacaggac ttgttccccg
     9361 gcagtatagc actggaggaa aatcgactct gttgggtatc agtaagcgag gtgataaaaa
     9421 tttacgccgg ttactggttc agtgtgcccg ctcattcata atgcgacttg aacgcaagca
     9481 tggaaaactt gctgagtggg ttcgggagca actcaacaag aagcattcga atgtggttac
     9541 ctgcgctctg gcaaataaac tggctcgaat tgcctgggca atcactactc agaaaaaaga
     9601 atatcaggcc tgatatagat gctgatataa ctcaattagt ttattttaaa caataagata
     9661 caagttacct ttctggtttt gcgaagactg attattgatg acatgaacgg caaatcgacc
     9721 tgattaaaaa cctaacagat gaaatggctc actgaagccg aggggatttt taggattatc
     9781 aggtgcggaa ctcatcgggg cgcgggcatc cttgcccaca gagacgccgg atagatttaa
     9841 gcaagccaac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattcatc
     9901 ttatcaccgg acaacaggct gaccgactcg ccgatctgga aagaatgttg cacctgttca
     9961 gcggtaaacc aatccccgac aaccggggaa acatcaccct tcgtctggat gatcacattc
    10021 agtctgttca gggtaaagag agctatgaag atgagatgtt cagcatcaga tactttaaga
    10081 agggctccgc gcatatcacg ttcagaaaac cagaactggt tgacagactg aatgatatta
    10141 tcgcaaaaca ctatcctgat atgctggcag tatagctttg ccagtatgct caaagatcgg
    10201 aaattgtccg ataagatatg atcgcggggc atataaaccg gtttcagatg gcatacccga
    10261 tggcatactg tctggaagga aaaataaaaa agcgcatgtt aaacaataac atgcacttat
    10321 aatctggcgg tgaggggggg attcgaaccc ccgatacgtt gccgtataca cactttccag
    10381 gcgtgctcct tcagccactc ggacacctca ccaaattgtt ttgctaccaa acctcatggg
    10441 tggcaacggg gcgctactat agggagttgg agtaaaacgg tcaagaagaa ttttaatgat
    10501 aattattgtt tgctcatact gtaaacaagt tgtgcagtat atctacatcg agacaagtta
    10561 cggacttata cttccaaagt acttcataca tatcacaaaa taaaaaggcc ggttaaaccg
    10621 accttttact cgttctttct cttcgcccat caggcggtaa aacaatcagc gactacggaa
    10681 gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca acagtcactt tgtcgcccgt
    10741 caggatgcgg atgtagtttt tgcgcatttt accggagatg tgtgcagtaa ccacgtgacc
    10801 gttttctaac tctacgcgga acatggtatt aggcaacgtt tcaagaacgg taccttgcat
    10861 ttcaatattg tcttctttgg ccatctaatc ctctggggta tcactaccgt aatttgaacc
    10921 ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt gcgcgctaaa tcgcaacaaa
    10981 caggttcggc acattactcc gaaaacacac ggctaagccg caccaaaagc gcaacgtata
    11041 agggagcggt gagataaacg atgggcgtta cctgacgcga aaaattcctt atcggcagcg
    11101 gggtaatgag cgtaaccaac tctgcgaccg caattataac actctgggga gaaatgtgcc
    11161 gaaaacattc attcttgtgg tgaaaacaag catcgtggta cccagaaatt attcggcaat
    11221 cgtccgaggc gcatttgatt gagataatta aggtaatccc ggcggggaat ttcgcaggca
    11281 ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga taagcttacc gccatgaccg
    11341 ataaattcct cacagaatac cagaagcgcc gttttagacg cattttccat ccggctgaac
    11401 atggactcgc cacaaaatag cgttccctgg gccacgccgt acataccgcc gacaagctca
    11461 tcttcacgcc agacttcaat ggagtgggca tggccgagtt cgtgaaggcg atggtaggct
    11521 tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat cgctggcaca gccttcaatg
    11581 acctgaccaa aagcgtaatt catcgtgaca cgatagggcg agcgtttatg aaatcgcttc
    11641 atactacggc tgatatgcag tgattctggc catagcaccg cgcggggatc gggcgaccac
    11701 cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac cacgctggta agccattaac
    11761 aggcgcgcag ggctaagatc gcccccaagt gccagcaggc cgttaggctc acgtaatgcg
    11821 ccttccgggg aagggaaggc tattgaatgg cgagaaagct gaaccaggcg catgaccgca
    11881 aaactccacg caagtcggat cgttcaataa tagcttacaa accctgcttg aactggtaat
    11941 aacgcccctg tctggcaagc agctctgcgt gagtaccttg ctcaataatt tgcccgttgt
    12001 ccatcactat tatttgttgg aaacgagaga gtccgcgaag tcgatgggtg accattaaca
    12061 ccgttttctc acgcatcatt tctgcaagca attcaaggat ctggctttcg gttgtggcat
    12121 ctaagccttc ggtaggttca tccagcaaca ccagtggcgc atcatgtaac agcgcacggg
    12181 cgatagccag acggcgcagt tcaccaccgg agagctggcg tccgccttca cctaaccaac
    12241 tgttgagacc tgcatcctcg agcagcttct ccagaccaac gcgacgcaag ctctccgcca
    12301 gagcctcatc actactgcca ggcgaggcga gtaacagatt atcacgcagc gtggcgctaa
    12361 acagatgcac tcgctgagga acaacgctga tggtctgtcg tagagccgct tcattcaggc
    12421 tggctatggg gctatcgtta agcaaaatct cgccctgttg cgggtcccaa gcgcgggtca
    12481 gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc gagaatcgca atatgttccc
    12541 cggcgtttac ctgaagagaa atccctttaa gtgcctgttg ggattgctcc ggataagtga
    12601 actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac acgagtttgg gtatcaggga
    12661 aggtgacttc cggtttttga tccgttaaat cagtgatccg aacggcagag gcaatgactt
    12721 gccccagatg ctgaaatgca cccgttactg gtgccagtgc ttcaaacgcg gctaacgcgc
    12781 agaagacaaa cagggcaatt aacgcgccgg gttgagcatt gccgccaacg ccgccagacg
    12841 ccatccacag catcaggatc accgctaacg cgccaatgag cagcattatc gcttgcgaca
    12901 atgcggtcag ttcagattga cggcgttgcg cttccagcca ttgaatttct gtattctcta
    12961 gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag ctcagcttgc ccttgcagcc
    13021 aggccgtcag ttgttggcga tactgtccgc gaagatgagt cagattttgc ccggtgcttt
    13081 ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt cagtaacata atgccgccca
    13141 gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat tgtcaccatc ataatcacca
    13201 caaaagcgcc caccagcggc gagataacgc gcaggtaaag atgatcgagc gtatcaacat
    13261 ccgccaccac gcgattgagc aattcgccct gacgatagcg cgccagtccg gcaggggaga
    13321 ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg caacacgcgg aaagtcgcgt
    13381 cgtgacttac cagacgttca aaataacgcc cggcagtacg ggtgattgcg gcaccacgca
    13441 cgcccgcagc gggtagcata tagttgaagc tgtacagtcc ggcaaccccc gcaaccgctg
    13501 aggccgagag gaaccagccg gaaagtgtca acagtccgat actggcgagc agcgtcacaa
    13561 ttgccagcac aataccaaga cttaacatcc atttatgacg tttatacagt gctagatagg
    13621 gtagcaaagc gcgcatttaa atctcctcct gacgatgggc cagtaatgtg gcgaatgggc
    13681 caccagccac acttaattcc gcgtaacgtc cttgctcaat aatccggcca tcctgcataa
    13741 cccaaatgac atcccagtca gcaagatctt ctaactggtg ggtgaccatt aacgttgtct
    13801 ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg ttcactgtga gcatcaaggc
    13861 tggcagcggg ttcatccaac agtaatagcg aacagggatt tagtaacgca cgggccaccg
    13921 ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg gtcgccaaca ggcgtatcaa
    13981 cgccttgtgg caggagcggt agaaactcgc tgacccaggc gttatccagc gctgcttgta
    14041 attcatgttc gctggcatca ggtcgcgcca gtagtacgtt atcccgtaat gttgctgccg
    14101 gtaattgtgg attttgccca acccaggaga gatgtttacg ccatgattct ggtgataaat
    14161 cgcgtaattc tatcccgttg attcgtagcg atccctgata tgagagaaaa ccagaaagcg
    14221 cgttcaacag tgaactttta cctgaaccgc tgcgaccaac caacacggca cgttggcctg
    14281 ctggcaaagt aaagttcagc ggtccggcca gcgttttacc ttccggcgac gtgataaaca
    14341 gatcctcggc ctcaatggtc accggatcgg tcgatgctaa ttccacctcg ccacgctgcg
    14401 gatgggcgag cggggtttcc ataaacgttt tcagactgtc agctgcacca acagcctggg
    14461 ctttagcatg ataaaacgta ccgagatcgc gtaatggctg gaaaaactct ggcgcaagga
    14521 tcagggccag aaaacccgca gccagcgtca cgccagtatc gtagtgacca aaatccagct
    14581 cgccgagata ggaaaaacca aagtagaccg ccaccagagc aattgacagc gaggtaaaaa
    14641 attcgagaat gccggaggat aaaaatgcca gccgtagcac ttccattgtc cgttggcgga
    14701 aatcttccga agcagaacga atactttcaa tttcagcttc accacgacca aaaatacgca
    14761 atgtttccat gccgcgcagg cgatcgagga aatgcccact taagcgagca agagcgagaa
    14821 agttacgtcg gttagcatcg gcagccccca ttccaaccag cgccataaac aacggaatta
    14881 acggtgcagt gcccagcaga atgagcgccg cagcccagtt tgaggggaag attgccacca
    14941 caatcagcaa cggcaccgac actgccagcg ccatttgcgg caggtagcgt gcatagtaat
    15001 catgcatatc gtcaatttgc tcgagtacca gcgtcgccca gctccccgca ggtttaccct
    15061 gaatccacgc tggccctgct tgttgcagac ggtcgagaac ctgccggcgg atggcaaagc
    15121 ggatatgctg cccggcgtga taacccaccc gttcgcgtaa ccagaccacc catgcgcgca
    15181 gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag ggcttcacgg ggaatattct
    15241 ccataatcat atgttgcaga attcgcgcca tgaaccaggc ctgggcaatg atcaatatgc
    15301 cgctcacaaa gcccagcaga cgagaaatat tcagccaacg ttgggagatg acgctttgct
    15361 gttttaacca gcgggttaac tctttttgac gagatttatt cattgcacgc ttagcaggtg
    15421 agttatcaga attatttgca gagcaatgtt acaacgggga aaaaataaag gcgacccata
    15481 gtcgcaaggt gtcgccttct ttacttttgt tactgatttg taaaattatt ttgcgtcagc
    15541 taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg cagcctgtac cggccgaagt
    15601 aatggcctga cgataaatat gatccatcac gtcacccgcg gcaaagacgc caggaatgct
    15661 ggtctgggtg gcattaccat gaatacccga ctgtactttg atgtagccgt tttccagttc
    15721 cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg atagcaacaa acagaccggc
    15781 aacgtcgagt gattcgatgt tatcgctgtt ttgcgtatcg cgcagacgaa cgccagtgac
    15841 acccatttga tcgccggtca cttcttccag cgtccggttg gtgtgcagaa tgatgttgcc
    15901 gttctccact ttatccatca gacgcttaat gaggattttt tccgcgcgga aaccgtcacg
    15961 gcggtgaatc agatgtactt ccgaagcgat gttagacaga tacaacgcct cttcaaccgc
    16021 ggtattgccg ccgccgatga ccgcaacttt ctggttgcga tagaagaaac cgtcgcaggt
    16081 tgcacaagca gaaaccccac ggcctttaaa ggcttcttca gagggcaggc cgagatagcg
    16141 tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg tattcgccgt tatcgccatt
    16201 cagacggaac ggacggtttt gcagatccac cttgttgata tgatcaaaaa tgatttcagt
    16261 ttcaaatttg gtggcatgtt cgtgcatgcg ctccattaat aacggaccgg tcagatcgtt
    16321 tggatcgcca ggccagtttt ccacttctgt ggtggtggtc agttggccgc ctttttccat
    16381 gccggtaatc agcacaggtt gcaggttggc gcgcgccgca tagacagcag cggtgtatcc
    16441 cgccgggcct gaacccagga taagcagttt actgtgtttg gtcgtgccca tgagatcccc
    16501 atagttgttg gcagacaatg ggcaggattg tagggaattt actgacgtaa aaaaagagta
    16561 tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga taagcaggtc aaattctccc
    16621 gtcattatca cctctgctac ttaaatttcc cgctttataa gctgattaaa tgatgaataa
    16681 acgcccctgt taatgaatat ctggcatgtt gtactaaaaa tcgatgtttt gctttgacaa
    16741 tcccctagtg ttttgcgaaa acattcgagg aagaaaaaaa acagtattct tatatgcgca
    16801 taaccatgca tgtaaatacc atgtttaccg tgctagtgaa atctacgtat ggcgtggaca
    16861 gacgccattc gtgatgtcga tagctgccac aaggcaacgg tcttctcacc gtagacccag
    16921 gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt gacgggtagc gactctgagc
    16981 agtgatgttt cagggtcaga caggagtagg gaaggaatac agagagacaa taataatggt
    17041 agatagcaag aagcgccctg gcaaagatct cgaccgtatc gatcgtaaca ttcttaatga
    17101 gttgcaaaag gatgggcgta tttctaacgt cgagctttct aaacgtgtgg gactttcccc
    17161 aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa gggtttattc agggctatac
    17221 ggcgctgctt aacccccatt atctggatgc atcacttctg gtattcgttg agattactct
    17281 gaatcgtggc gcaccggatg tgtttgaaca attcaatacc gctgtacaaa aacttgaaga
    17341 aattcaggag tgtcatttag tatctggtga tttcgactac ctgttgaaaa cacgcgtgtc
    17401 ggatatgtca gcttaccgta agttgctggg ggaaaccctg ctgcgtctgc ctggcgtcaa
    17461 tgacacacgg acatacgttg ttatggaaga agtcaagcag agtaatcgtc tggttattaa
    17521 gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt gattacactc ctgttaatcc
    17581 atacagcaac agtactgggg taacctggta ctgttgtccg ttttagcatc gggcaggaaa
    17641 agcctgtaac ctggagagcc tttcttgagc caggaataca ctgaagacaa agaagtcaca
    17701 ttgacaaagt taagtagcgg ccgtcgcctt ctggaagcgt tgctgatcct tattgtcctg
    17761 tttgccgtct ggttgatggc tgccttacta agctttaacc cttcggaccc cagctggtcg
    17821 caaacggcct ggcatgaacc tatccataat ttaggtggga tgcccggtgc gtggctggca
    17881 gatacgctgt tctttatttt tggcgtgatg gcttacacca ttcccgtcat tattgtcggc
    17941 ggttgttggt ttgcctggcg tcatcagtcc agtgacgaat acattgatta ttttgccgtt
    18001 tcgttacgca tcattggcgt tttggcgctc atccttacct cctgtggtct ggcggcaatc
    18061 aacgctgacg atatctggta ttttgcctcc ggtggcgtca ttggcagctt actaagcact
    18121 acgttacaac cactgctaca cagtagcggg ggaactattg cgctgctctg cgtttgggct
    18181 gcgggcctga cgctgttcac cggttggtca tgggtgacca ttgctgaaaa actcggcggc
    18241 tggattttaa acattctcac cttcgccagt aatcgtaccc gtcgcgatga tacctgggtc
    18301 gatgaagatg aatatgaaga tgacgaagag tatgaagatg aaaaccacgg caaacagcat
    18361 gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc gtcgtaaacg gttggcggaa
    18421 aaattcatta atccgatggg gcggcaaaca gacgcagcgt tgttctccgg taagcggatg
    18481 gatgatgaag aagagattat ctacactgca cgcggtgtgg ctgccgaccc ggacgacgtc
    18541 ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg aatacgatcc attattaaac
    18601 ggtgcgccaa ttaccgaacc tgttgctgta gcagctgctg ctaccacggc gacacaaagc
    18661 tgggctgcgc cggttgaacc tgtgacccag acgccgcctg ttacttctgt tgatgttcca
    18721 ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc cacaaacggg agagccggtt
    18781 attgctcctg caccggaagg ttacccacag cagccacaat atgcgcagcc tgcagtgcaa
    18841 tataatgagc cgctgcaaca acctgtacag ccacagcagc cgtattatgc gcctgcagct
    18901 gaacaacctg tgcaacaacc ttactacgct cctgcggcgg aacaacctgt tcagcaaccg
    18961 tattacgcaa ctgcgccgga acagcctgcg caacagccgt attatgcccc tgcgccagaa
    19021 caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt tgctccacag
    19081 tctacatacc agactgagca aacttatcag cagccagccg ctcaggagcc gttgtaccaa
    19141 cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt agaagagaca
    19201 aaacccgcgc gtccgccgct ttactatttt gaagaagtgg aagagaagcg agcccgtgaa
    19261 cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga accagaaccg
    19321 atcaaatctt cgttgaaagc gccttctgtt gcagcagtac ctccagtaga agccgctgcc
    19381 gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg ggctgccgca
    19441 accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc gcaagtcaaa
    19501 gagggaattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac tcgtcgtgaa
    19561 ctggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagagaa agcccgtgaa
    19621 gcccggcgca atcagtatga ttctggcgat cagtataacg atgatgaaat cgatgcgatg
    19681 cagcaggatg aactggcacg tcagttcgcc caggcacagc agcaacgcta tggcgaacag
    19741 tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga ggctgaactg
    19801 gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc ggctggggcg
    19861 aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct ggatgatggt
    19921 ccacacgaac cgttgtttac gccaattgtt gaacctgtac agcagccgca acaactggtt
    19981 gcaccgcagc agcagtatca gcagccacaa cagccggttg cgccacagca gcaatatcag
    20041 cagccgcaac agccggttgc gccgcagcag cagtatcagc agccacaaca gcaggttgcg
    20101 ccgcagccac aatatcagca gccgcaacaa ccggttgcac cgcagcagca ggataccctg
    20161 cttcatccgc tgttgatgcg taatggcgac agccgtccgt tgcataaacc gacgacgccg
    20221 ctgccttctc tggatttgct gacaccgccg ccgagcgaag tggagccggt agataccttt
    20281 gcgcttgaac aaatggctcg cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc
    20341 gatgtcgtca attactctcc ggggccggtt atcactcgct ttgaacttaa cctggctcct
    20401 ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc ttgcccgttc actttcgacg
    20461 gtggcggtgc gtgtcgttga agttattcct ggcaaaccct atgtaggtct ggagttacca
    20521 aataaaaaac gacaaaccgt ttatctgcgc gaagttttgg ataacgccaa attccgcgat
    20581 aatccgtcgc cattaaccgt ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc
    20641 gatctggcga aaatgccgca cttgttggtt gcggggacta ccggttccgg taaatctgtc
    20701 ggtgtgaacg cgatgatcct gagcatgctt tataaagcac agccagaaga tgtgcgtttc
    20761 atcatgatcg acccgaaaat gctggagctt tcggtttatg aaggcattcc gcatctgtta
    20821 acggaagtcg ttactgatat gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag
    20881 atggagcgtc gttataaact gatgtctgcg ctgggtgtgc gtaatctggc gggttataac
    20941 gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc cagacccgta ctggaagccg
    21001 ggtgacagta tggatgccca gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg
    21061 gttgacgaat ttgccgacct gatgatgacg gtaggtaaaa aagtggaaga gctgatagca
    21121 cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg tactggcaac tcagcgtcca
    21181 tcggttgatg ttattactgg tctgattaaa gcgaatattc cgacccgtat cgcctttacc
    21241 gtatccagta agattgactc acgtaccatt cttgatcagg ctggcgcgga atcactgctg
    21301 gggatggggg atatgctcta ctctgggccg aactccacgt tgccggtacg tgtccatggt
    21361 gcttttgttc gcgatcagga agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc
    21421 ccacagtatg ttgatggcat cacttccgac agtgaaagcg aaggtggcgc gggcggtttc
    21481 gatggcgctg aagaactgga tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa
    21541 cgcaaagcgt caatttctgg cgtacagcgt cagttccgca ttggttataa ccgtgcagcg
    21601 cgtattatcg aacagatgga agcgcagggg attgtcagcg aacaggggca caacggtaat
    21661 cgtgaagtgc tggccccacc gccgtttgac taattaatgc attgccggat aaggcgcggt
    21721 agcgtcgcat ccggcactct atcaactgaa aattcagtat tttctttttt cctcaagctg
    21781 attattagcc tggaatagag agtagaggga actcccgatc gggagtgacg taatttgagg
    21841 aataatgatg aaaaaaattg ccatcacctg tgcattactc tcaagcttag tagcaagcag
    21901 cgtttgggct gatgccgcaa gcgatctgaa aagccgcctg gataaagtca gcagcttcca
    21961 cgccagcttc acacaaaaag tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg
    22021 cgatctgtgg gtgaaacgtc caaacttatt caactggcat atgacacaac ctgatgaaag
    22081 cattctggtt tctgacggta aaacactgtg gttctataac ccgttcgttg agcaagctac
    22141 ggcaacttgg ctgaaagatg ccaccggtaa tacgccgttt atgctgattg cccgcaacca
    22201 gtccagcgac tggcagcagt acaatatcaa acagaatggc gatgactttg tcctgacgcc
    22261 gaaagccagc aatggcaatc tgaagcagtt caccattaac gtgggacgtg atggcacaat
    22321 ccatcagttt agcgcggtgg agcaggacga tcagcgcagc agttatcaac tgaaatccca
    22381 gcaaaatggg gctgtggatg cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt
    22441 agatgatcaa cgtaagtaga ggcacctgag tgagcaatct gtcgctcgat ttttcggata
    22501 atacttttca acctctggcc gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc
    22561 agcaacattt gctggctgcg gggaagccgt tgccgcgcgc tatcgaagcc gggcatctgc
    22621 attctatgat cctctggggg ccaccgggta ccggcaaaac aactctcgct gaagtgattg
    22681 cccgctatgc gaacgctgat gtggaacgta tttctgccgt cacctctggc gtgaaagaga
    22741 ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc aggtcgccgc actattcttt
    22801 ttgttgacga agttcaccgt ttcaacaaaa gccagcagga tgcatttctg ccacatattg
    22861 aagacggcac catcactttt attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt
    22921 cggcactgct ttcccgtgcc cgtgtctatc tgttgaaatc cctgagtaca gaggatattg
    22981 agcaagtact aactcaggcg atggaagaca aaacccgtgg ctatggtggt caggatattg
    23041 ttctgccaga tgaaacacga cgcgccattt ctgaactggt gaatggcgac gcgcgccggg
    23101 cgttaaatac gctggaaatg atggcggata tggccgaagt cgatgatagc ggtaagcggg
    23161 tcctgaagcc tgaattactg accgaaatcg caggtgaacg tagtgcccgc tttgataaca
    23221 aaggtgatcg cttttacgat ctcatttccg cactgcataa atcggtacgt ggtagcgcac
    23281 ccgatgcggc gctgtactgg tatgcgcgaa ttattactgc cggtggcgat ccgttatatg
    23341 ttgcgcgtcg ctgtctggcg attgcgtctg aagatgtggg taatgctgat ccacgagcga
    23401 tgcaggtggc aattgcagcc tgggattgct ttactcgcgt tggcccggcg gaaggtgaac
    23461 gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc aaaaagcaac gctgtctaca
    23521 ctgcgtttaa agctgcgctg gcggatgctc gcgaacgtcc ggattatgac gtgccggttc
    23581 atttgcgtaa tgcgccgacg aaattaatga aggaaatggg ctacgggcag gaatatcgtt
    23641 acgctcatga tgaagcaaac gcttatgctg ccggtgaggt ttacttcccg cgggaaatag
    23701 cacaaacacg ctattatttc ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc
    23761 tcgcctggct ggctgaacag gatcaaaata gccccataaa acgctaccgt taatgttatc
    23821 gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc tgtggccaca tctcccattt
    23881 aattcgataa gcacaggata agcatgctcg atcccaatct gctgcgtaat gagccagacg
    23941 cagtcgctga aaaactggca cgccggggct ttaagctgga tgtagataag ctgggcgctc
    24001 tcgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact
    24061 cccgatcgaa atccattggc caggcgaaag cgcgcgggga agatatcgag cctttacgtc
    24121 tggaagtgaa caaactgggc gaagagctgg atgcagcaaa agccgagctg gatgctttac
    24181 aggctgaaat tcgcgatatc gcgctgacca tccctaacct gcctgcagat gaagtgccgg
    24241 taggtaaaga cgaaaatgac aacgttgaag tcagccgctg gggtaccccg cgtgagtttg
    24301 actttgaagt tcgtgaccac gtgacgctgg gtgaaatgca ctctggcctc gactttgcag
    24361 ctgcagttaa gctgactggt tcccgctttg tggtaatgaa agggcagatt gctcgcatgc
    24421 accgcgcact gtcgcagttt atgctggatc tgcataccga acagcatggc tacagtgaga
    24481 actatgttcc gtacctggtt aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat
    24541 ttgctggcga tctgttccat actcgtccgc tggaagaaga agcagacacc agtaactatg
    24601 cgctgatccc aacggcagaa gttccgctga ctaacctggt gcgcggtgaa atcatcgatg
    24661 aagatgatct gccaattaag atgaccgccc acaccccatg cttccgttct gaagccggtt
    24721 catatggtcg tgacacccgt ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg
    24781 tgcagatcgt gcgcccggaa gactcaatgg cggcgctgga agagatgact ggtcatgcag
    24841 aaaaagtcct gcagctgctg ggcctgccgt accgtaaaat catcctttgt accggcgaca
    24901 tgggctttgg cgcttgcaaa acctatgacc tggaagtatg gatcccggca cagaacacct
    24961 accgtgagat ctcttcctgc tccaacgttt gggatttcca ggcacgtcgt atgcaggcac
    25021 gttgccgcag caagtcggac aagaaaaccc gtctggttca taccctgaac ggttctggtc
    25081 tggctgttgg tcgtacgctg gttgcagtaa tggaaaacta tcagcaggct gatggtcgta
    25141 ttgaagtacc agaagttctg cgtccgtata tgaacggact ggaatatatt ggctaatacc
    25201 caatttttct gaatctaaaa agcgcctgcg ggcgcttttt ttgtctccct ttgataccga
    25261 acaataatta ctcctcactt acacgtaata ctactttcga gtgaaaatct acctatctct
    25321 ttgattttca aattattcta tgtatacaag cctatatagc gagctgctat agaaataatt
    25381 acacaatacg gtttgtactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    25441 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    25501 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    25561 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    25621 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    25681 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    25741 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    25801 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    25861 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    25921 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    25981 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    26041 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    26101 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    26161 gcgaaacgcg aatgagtggc agtggggtga cttactatct tgaacaggca cgccagaaat
    26221 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    26281 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    26341 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    26401 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    26461 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    26521 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    26581 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    26641 tgctggcgat tctgaccggt aacgttggta ttaacggagg caacagcggc gcgcgtgaag
    26701 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    26761 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    26821 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    26881 gtaactgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    26941 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    27001 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    27061 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    27121 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    27181 ttactgaagg ccgtacacag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    27241 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    27301 acccacaagg gcaccacgtt gcttataaag ctttccgtga agatcctcag gcaaatccac
    27361 tgaccacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    27421 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccaggctttg
    27481 aaagttatca ggatccgttg aacaaacagt atccgctgca gcttacgggt ttccactata
    27541 aatctcgcgt tcactcaact tacggcgacg ttgatgtgct gaaagcagct tgccgtcagg
    27601 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    27661 ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc
    27721 cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatacaaaa cgtgtcgata
    27781 agggcggttg tatcaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    27841 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    27901 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcctgt
    27961 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    28021 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    28081 atttcatgta accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    28141 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctac
    28201 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    28261 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    28321 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    28381 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    28441 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
//
LOCUS       DAJTMK010000036        27707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTMK010000036 DAJTMK010000000
VERSION     DAJTMK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27707
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.037"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..452)
                     /locus_tag="OO135_001329"
     CDS             complement(<1..452)
                     /locus_tag="OO135_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5397245.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVKRLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLR"
     gene            complement(457..630)
                     /locus_tag="OO135_001330"
     CDS             complement(457..630)
                     /locus_tag="OO135_001330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR5397246.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(643..1881)
                     /gene="murP"
                     /locus_tag="OO135_001331"
     CDS             complement(643..1881)
                     /gene="murP"
                     /locus_tag="OO135_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5397247.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLHLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDLRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIA"
     gene            complement(1885..2781)
                     /gene="murQ"
                     /locus_tag="OO135_001332"
     CDS             complement(1885..2781)
                     /gene="murQ"
                     /locus_tag="OO135_001332"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5397248.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            2945..3802
                     /gene="murR"
                     /locus_tag="OO135_001333"
     CDS             2945..3802
                     /gene="murR"
                     /locus_tag="OO135_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5397249.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            3931..4722
                     /gene="ucpA"
                     /locus_tag="OO135_001334"
     CDS             3931..4722
                     /gene="ucpA"
                     /locus_tag="OO135_001334"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5397250.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNLEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            5026..6042
                     /gene="cysP"
                     /locus_tag="OO135_001335"
     CDS             5026..6042
                     /gene="cysP"
                     /locus_tag="OO135_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5397251.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            6042..6875
                     /gene="cysT"
                     /locus_tag="OO135_001336"
     CDS             6042..6875
                     /gene="cysT"
                     /locus_tag="OO135_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5397252.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAVSLLLLFSINTLQSRFGRRVVG
                     H"
     gene            6875..7750
                     /gene="cysW"
                     /locus_tag="OO135_001337"
     CDS             6875..7750
                     /gene="cysW"
                     /locus_tag="OO135_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5397253.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            7740..8837
                     /gene="cysA"
                     /locus_tag="OO135_001338"
     CDS             7740..8837
                     /gene="cysA"
                     /locus_tag="OO135_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5397254.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            8971..9882
                     /gene="cysM"
                     /locus_tag="OO135_001339"
     CDS             8971..9882
                     /gene="cysM"
                     /locus_tag="OO135_001339"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5397255.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     VLSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(9885..10253)
                     /locus_tag="OO135_001340"
     CDS             complement(9885..10253)
                     /locus_tag="OO135_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5397256.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            10358..11209
                     /gene="pdxK"
                     /locus_tag="OO135_001341"
                     /gene_synonym="thiJ"
     CDS             10358..11209
                     /gene="pdxK"
                     /locus_tag="OO135_001341"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5397257.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(11251..11760)
                     /gene="crr"
                     /locus_tag="OO135_001342"
     CDS             complement(11251..11760)
                     /gene="crr"
                     /locus_tag="OO135_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5397258.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
     gene            complement(11801..13528)
                     /gene="ptsI"
                     /locus_tag="OO135_001343"
     CDS             complement(11801..13528)
                     /gene="ptsI"
                     /locus_tag="OO135_001343"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5397259.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(13573..13830)
                     /gene="ptsH"
                     /locus_tag="OO135_001344"
     CDS             complement(13573..13830)
                     /gene="ptsH"
                     /locus_tag="OO135_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5397260.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(14214..15185)
                     /gene="cysK"
                     /locus_tag="OO135_001345"
     CDS             complement(14214..15185)
                     /gene="cysK"
                     /locus_tag="OO135_001345"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5397261.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(15370..16131)
                     /gene="cysZ"
                     /locus_tag="OO135_001346"
     CDS             complement(15370..16131)
                     /gene="cysZ"
                     /locus_tag="OO135_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5397262.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            16361..17347
                     /gene="zipA"
                     /locus_tag="OO135_001347"
     CDS             16361..17347
                     /gene="zipA"
                     /locus_tag="OO135_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5397263.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDYYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            17418..19433
                     /gene="ligA"
                     /locus_tag="OO135_001348"
     CDS             17418..19433
                     /gene="ligA"
                     /locus_tag="OO135_001348"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5397264.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRQMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLKALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            19435..19653
                     /locus_tag="OO135_001349"
     CDS             19435..19653
                     /locus_tag="OO135_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5397265.1"
                     /translation="MEKEQLIEMANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(19650..20648)
                     /locus_tag="OO135_001350"
     CDS             complement(19650..20648)
                     /locus_tag="OO135_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5397266.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            20738..21664
                     /locus_tag="OO135_001351"
     CDS             20738..21664
                     /locus_tag="OO135_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5397267.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(21655..21996)
                     /locus_tag="OO135_001352"
     CDS             complement(21655..21996)
                     /locus_tag="OO135_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5397268.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(22116..22191)
                     /locus_tag="OO135_001353"
     tRNA            complement(22116..22191)
                     /locus_tag="OO135_001353"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22156..22158),aa:Lys,seq:ttt)
     gene            complement(22196..22271)
                     /locus_tag="OO135_001354"
     tRNA            complement(22196..22271)
                     /locus_tag="OO135_001354"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22236..22238),aa:Val,seq:tac)
     gene            complement(22316..22391)
                     /locus_tag="OO135_001355"
     tRNA            complement(22316..22391)
                     /locus_tag="OO135_001355"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22356..22358),aa:Val,seq:tac)
     gene            22651..24066
                     /gene="gltX"
                     /locus_tag="OO135_001356"
     CDS             22651..24066
                     /gene="gltX"
                     /locus_tag="OO135_001356"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5397269.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            complement(24117..24509)
                     /locus_tag="OO135_001357"
     CDS             complement(24117..24509)
                     /locus_tag="OO135_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5397270.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(24511..24870)
                     /locus_tag="OO135_001358"
     CDS             complement(24511..24870)
                     /locus_tag="OO135_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5397271.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            25091..25166
                     /locus_tag="OO135_001359"
     tRNA            25091..25166
                     /locus_tag="OO135_001359"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25124..25126,aa:Ala,seq:ggc)
     gene            25206..25281
                     /locus_tag="OO135_001360"
     tRNA            25206..25281
                     /locus_tag="OO135_001360"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25239..25241,aa:Ala,seq:ggc)
     gene            25490..27679
                     /locus_tag="OO135_001361"
     CDS             25490..27679
                     /locus_tag="OO135_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR5397272.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALYQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFWIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
BASE COUNT         6633 a         6902 c         7410 g         6762 t
ORIGIN      
        1 atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa ccccggaata
       61 tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt ttgcagcgcg
      121 aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc ggtggtggct
      181 ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc ccaggctttg
      241 cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata accggcatca
      301 acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa ccctgctttc
      361 tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca actaacagcc
      421 agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga cgttacggca
      481 gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc cgccagcata
      541 aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg ctaccagacc
      601 agacggccca aaggcgctgt ttaagcccat cggcagaccc cactaggcta tcaggccgat
      661 aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca tgcggggcag
      721 ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa tcgccccgcg
      781 tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca gcgccgcgcc
      841 cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc cctggctgtc
      901 cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca cggcgatcag
      961 gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca taaacagcca
     1021 tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg caagcgtagc
     1081 ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg gcataaagcg
     1141 gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc cgataatatt
     1201 gccgcgcaga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt aaccggtggt
     1261 tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat tgacgcccgt
     1321 gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg tgaacaaacc
     1381 tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag catctgccgg
     1441 aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg cggcaataaa
     1501 accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt gcacgccgga
     1561 ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg catcctgtac
     1621 cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt taccaggtcc
     1681 aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt ccagcgtttt
     1741 gatattggga tcaaccagtg aactgtcatg tacacccaga tgcaggcgcg tcatacagtt
     1801 accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt tcagaagttc
     1861 actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa taaagccgcc
     1921 gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca ccatcacaat
     1981 ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt cggcattaca
     2041 tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt tggtggcgac
     2101 cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc cggtggaaag
     2161 catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac cggtaacaac
     2221 ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg cgctccccgg
     2281 attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca gtccggcaat
     2341 cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg ccgttaaatt
     2401 aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt ccaccgcgtg
     2461 ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt tcacgccgta
     2521 ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc ccgcaccgag
     2581 gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg ccgcggcgat
     2641 atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt tgataatccg
     2701 gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg agccttcagt
     2761 aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa taattgcaga
     2821 tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt ctacactcgc
     2881 agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc caggagagtg
     2941 gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg aaaacgagca
     3001 aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt cttcacggca
     3061 gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc aaaaactcgg
     3121 cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg ccagccggga
     3181 gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg attcgctgga
     3241 agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt
     3301 gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca
     3361 gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa
     3421 aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg tttcccaggc
     3481 gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggcagtaaaa aagagattgt
     3541 cttgtgcgca gaagcggcac gaaagcaggg ggcaacggtg attgccatta cttctctggc
     3601 tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg gtgaaaccga
     3661 atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg atttactgtt
     3721 tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc gcagcagtga
     3781 gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt ttcctgaatt
     3841 agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga atggagtgta
     3901 acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa gacagcactg
     3961 attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc acgtcatggc
     4021 gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga cgaactgtgt
     4081 ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc gtcggtagcc
     4141 gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt gaataacgca
     4201 ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga tttccatatt
     4261 gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga gatgattgcg
     4321 cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt ggccgatcct
     4381 ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa atcgctggcg
     4441 gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata cgtgcgcaca
     4501 ccaatggcgg aaagcattgc ccgccagtcg aacctggaag atccagagtc ggtgctgact
     4561 gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt cggcgaactg
     4621 gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa tgtgattgat
     4681 ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc tgtttcctcc
     4741 ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt tatttgtcat
     4801 tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg gtttctccgc
     4861 gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt cttattccct
     4921 ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac gtccgttata
     4981 aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc cgttaactta
     5041 ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca tgtacaggca
     5101 acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc cctgaatccg
     5161 ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat aaaacaatct
     5221 catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac cgacgttgtc
     5281 acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct gatcccggct
     5341 gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat gggcttcctg
     5401 gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg ctccgacgtg
     5461 aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta tctggcggca
     5521 tggggtgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca gtttatgacc
     5581 cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac caccactttt
     5641 gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa caacatccgt
     5701 aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat tctggcggaa
     5761 ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa agcagcaaaa
     5821 gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga ctattactac
     5881 cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca gaccgagctg
     5941 ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca cttcaccagc
     6001 ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca gtttcatcaa
     6061 gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt gtgtgcctga
     6121 ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc tgggcgcagt
     6181 actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg ctgctgtcgg
     6241 cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg attttaacac
     6301 gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc tttgcgctgc
     6361 caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt ttttacggtg
     6421 aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt gcggtggcga
     6481 tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg gaagagttag
     6541 gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag agtttctgca
     6601 aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg tcgtttaccc
     6661 gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgcc tggaagacgg
     6721 aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg gcggcgagcg
     6781 cgattgcttc ggtgatcctc gcggtatccc tgctgctgct gttctcaatt aacactctgc
     6841 aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc aattgaagcg
     6901 ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg ggatgctggt
     6961 atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt tcagcaaagg
     7021 gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca tttggctgac
     7081 ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc tgctggcgtg
     7141 gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac tggacattcc
     7201 gtttgccgta tcgccggtgg ttgccggtct ggtgtatctg ctgttctacg gctctaacgg
     7261 tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct ggccgggaat
     7321 ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg tgccggtgat
     7381 gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt ctggctggca
     7441 gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg gtgtggtgct
     7501 aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg gctcgattcg
     7561 cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact acaacaccgt
     7621 cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc tgtttttaaa
     7681 gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg aggagcatca
     7741 tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg ctgaacgata
     7801 tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc ggttccggaa
     7861 aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg catattcgct
     7921 tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt ttcgtgttcc
     7981 agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt ggcctgacgg
     8041 tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg acaaaattgc
     8101 tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt tccggcggcc
     8161 agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt ctcctgcttg
     8221 atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc tggctgcgtc
     8281 aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag gaagaagcga
     8341 ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag gctgatgcgc
     8401 cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg ggcgaagtga
     8461 accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat cgctggccgc
     8521 tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg tgggaagtgg
     8581 atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg gaagccagcc
     8641 cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac gaaccgctga
     8701 cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc gttggtctgc
     8761 aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag gaacttgctc
     8821 tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc ttcccgcgat
     8881 ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg caagcgtcgc
     8941 atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac aataggcaat
     9001 acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt ctggttgaaa
     9061 ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggtactttc gatgatcgtc
     9121 gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc caccagtggt
     9181 aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat gaaattgctg
     9241 atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg tgcggaactg
     9301 attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct ggagatggcg
     9361 aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc ttacgcccat
     9421 tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac ccattttgtc
     9481 tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg cgaacaatcc
     9541 aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc cggcattcgc
     9601 cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga tgaggtgctg
     9661 gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg ggaaggaata
     9721 ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc aaaagctaac
     9781 cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct ctccaccgga
     9841 gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg cgcaggcacc
     9901 gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc atcgctctta
     9961 ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc aaccttatca
    10021 ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag acgcagatga
    10081 gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa atcctttttc
    10141 tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt taacttcgcg
    10201 taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt cattgaatta
    10261 ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag tttcggttgg
    10321 aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt tgttgtttaa
    10381 cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg tggtttacgg
    10441 cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga atgtttttgc
    10501 cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg gtggtgcgat
    10561 tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg cgctgcgcca
    10621 gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa tccttgccga
    10681 gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg atccggtgat
    10741 tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt atcgacaata
    10801 tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa tcctgaccgg
    10861 taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc tttcagacac
    10921 attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg agatgcaggt
    10981 ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa aaaccgacct
    11041 gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga aagggaaggc
    11101 gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc gctacaccca
    11161 gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa aaatggcgcc
    11221 gatgggcgcc atttttcact gcggcaagaa ttacttcttg atgcggataa ccggggtttc
    11281 acccacggtt acgctaccgg gcagtttgat cagttctttg atttcgtcca tgttggagat
    11341 aacaaccgga gtcagggtag acttggcttt ctcttccagc agcggcagat caaattcaat
    11401 gacagtatcg ccaactttca cgcgctgacc ttcttctgca atacgcttga agccttcgcc
    11461 tttcagttca acggtgtcga taccgaagtg gacgaacagt tcaacgccgc tatcagattc
    11521 gatagagaac gcgtgattgg tttcaaagat tttaccaatg gtaccgtcta caggcgcgac
    11581 cattttgtta cccgttggtt tgatagcaat accatcacca acgatttttt ccgcaaaaac
    11641 gacatccggc acgtcttcga tattgacgat ctcgccagag agcggagcaa tgatctcaat
    11701 agttccggta tccttcttgt cgtcggaaac cagagatttc agtttatcga acaaacccat
    11761 gatcttctcc taagcagtaa attgggccgc atctcgtgga ttagcagatt gttttttctt
    11821 caatgaactt gttaaccagc gtcattaact cgtccgttgt cggttgagca agagcctgct
    11881 ctgctaacac cttcgcatct tcgaagttcg tgttacggat aatcttctta atgcgcggga
    11941 tagaaatggc gctcatagag aattcgtcca gacccatccc cagcaacaga agtgtagcac
    12001 gttcatcgcc agcaagctca ccacacatgc cagtccattt gccttcagca tgagaagcat
    12061 caataacttg cttgatcaag ttcagcacgg acggtgacat tggctggtaa aggtgtgaaa
    12121 tcatatcatt accacggtca actgccagag tgtactgcgt taaatcattg gtgccgatac
    12181 taaagaaatc aacttctttg gctaaatgac gtgcaattgt tgcggcagcc ggtgtttcca
    12241 ccattacgcc gatttcaatt gactcgtcaa acgctttacc ttcgtcgcgc agttcctgtt
    12301 tgtagatttc gatctctttg cgcagtgcac gcacttcttc aacagagatg atcatcggga
    12361 acataatgcg caatttaccg aaagccgagg cacgcaggat agcgcggagc tgatcgcgca
    12421 ggatctcttt acgatccatc gcgatacgga tagcgcgcca gccgaggaac gggttctctt
    12481 ctttcgggaa gttcatgtat ggcagctctt tgtcgccgcc gatgtccatg gtacgaacga
    12541 taaccgcctg cgagccacac gcttcagcca ctgctttgta agcagcaaac tgttcttctt
    12601 cagtgggcag cgcgtcgcgg tccatgaata ggaactcagt acgatacaga ccaacgcctt
    12661 cagcgccgtt acgctctgca ccttcaacgt cacgaaccgt accaatgtta gcgcatactt
    12721 ctacctggtg accgtccagc gtaatagctg gcagatcttt cagtttagca agctctgctt
    12781 tttcagaagc cacttgctcc tgaacagcgc gcattttatc aataacttcg ttggttggat
    12841 tgacgtaaac ctgattattt acggcatcca gaatcagata gtcgtcattt ttcacctgag
    12901 aggtgacgct accggtaccc acgatagcag gcagttccag agaacgcgcc atgatagagg
    12961 tgtgggaagt acggccaccc gcgtcggtga tgaaacccag caccttcttc aggttcagct
    13021 gtgcggtttc ggacggcgtc aggtcagcgg caaccagaat gacttcatcc tgaatggcgc
    13081 tcaggtcgat aatcttcagg cccaggatgt tgcgcagcag gcgcttaccg atatcacgta
    13141 cgtcagccgc acgttctttc aggtattcat catccagctc ttccagggca gaagcctgac
    13201 cttcgataac ttcatgagca gctgcgtcag ctgtcatgtg tttatcttta atcagggcta
    13261 tgatttcctg ctccagctcc tcatcttcga gcagcataat atgcccttca aagatggctt
    13321 ctttttcttc accgaacgtt tcaccagctt tcgttttgat cgtttccagc tgggctgatg
    13381 ccttggcacg accgctcaga aaacgttcaa cttcctgatc aacctggtcg gcagaaattt
    13441 ttttccggtc aatgacaatt tcgtcttctt tcagaagcag agctttaccg aaagcgatac
    13501 ccggggatgc taaaatgcct gaaatcataa ccctacctta cttgtgactg atttttaaaa
    13561 gaacccggga aattactcga gttccgccat cagtttaacc agatgttcaa ccgctttttg
    13621 ctcgtcttcg ccttctgcgg agatagtcac aacggtacct tgagtcaggc ccagagtctg
    13681 cagtttaaac aggcttttcg cgctggcgct tttgccgttg gaagtcacag taatttcaga
    13741 agtgaagccc ttagcttctt ttacaaactg ggcagcaggg cgggtgtgca gaccgttcgg
    13801 agctgtaatg gtaacttctt gctggaacat tgtatttccc caacttatag gtttagtgtt
    13861 gtggaactaa agtctagcct ggcggttcga ctttagcctg tattgttagc gccagttttt
    13921 aatagacgcg acgcacgaag agcgcaatac agtccattgc tggcgggtgt tgaccgctga
    13981 cgtttcgtac gtcattaaac attatgccgc taaaggaaga attgaaccaa atcataaaat
    14041 cgattcagct ttggcttttc ctgtaacgat taatttcgcg gatcaaaata attagtctgg
    14101 ttaaatacca gttccgacag gctgaatcaa tgccaggagg ggcgaaagtt tgaagcaggc
    14161 cacaaaaaag cacctaaaaa ggtgcttttt tacgcatttt taacaagctg gcattactgt
    14221 tgcaattctt tctcagtgaa gagatcggca aacaatgcgg tgcttaaata acgctcaccc
    14281 gatgatggta gaataaccac aatattcttg ttggtaaagc tttcatcttc ttgtagtttc
    14341 aacgccgcgg caacagctgc tccagaagag atacctgcaa gaataccttc ttcttccatt
    14401 agacgacggg cggtagaaat ggcttcttca ttggtgatgc caatgacttt atcgaccagc
    14461 ttgagatcga ggttagccgg gataaaacca gcgccaatac cctgaatttt atgcgggcca
    14521 ggtttaatct cttcacctgc cagcgcctgg gcgataactg gagaatcggt tggctcaacg
    14581 gcgacagaga taagatcggt cttgcctttg gtgcctttaa tgtagcggct gacgccagtc
    14641 agcgtaccgc cagtcccaac gccagcaata aatacatcaa cctgaccgtc ggtatcttcc
    14701 cagatctccg gaccggtggt tttttcgtga atttcagggt ttgccggatt gctgaattgt
    14761 tgcagcagca ggtatttctc tggattgctg gcgacaattt cttctgcttt ttggattgcg
    14821 cctttcatgc ctttagcacc ttccgtcagc accaggtttg cacctaacgc tttcagcagc
    14881 ttgcggcgtt caatactcat ggtttctggc atggtcaggg tgagtttgta accgcgagcg
    14941 gcagctacat aggccagtgc aatcccggta ttaccgctgg tcggttcaac cagttcaacg
    15001 cctggtttca gcacgccgcg cttttcggca tcccaaatca tgttggcacc gatacggcac
    15061 ttaacgctga agctggggtt acgagattcc accttcgcca gaatgcgtcc gttaccgatg
    15121 cgattcaggc gaaccagcgg cgtgtgaccg atagtcagcg agttatcttc aaaaatctta
    15181 ctcatggcct gtccttaact gtatgaaatt gggatacaac aggtagcata cccgctcaga
    15241 gaatatgcgg aagtaaggat ttagcatatc tatatacaga agggaaataa tgacatgcaa
    15301 gatggaataa ggggcggcat aagccacccc tgtttcacac aaacggttta caaaataacc
    15361 ggtagattgt taccgccaca tcgcgtgttt atcgcgatag caatcgaccc acatcgccgt
    15421 cgcgccacaa acggcaacgg gcatgatgaa cagattaagc agcgggatca tcgtaaacag
    15481 gctggttaaa gcaccaaact gcatattggt gattttgcgt gtgcgcaggg cggtgcgcat
    15541 ctctttaaac ggcactttgt ggttatcgaa ggggtaatcg caatactgga tggctaacat
    15601 ccaggcgcta aacaggaacc acagtaccgg cgcgacggtt tgcccaatac cagggatgaa
    15661 gtaaagaatt agtaatacaa ttgcgcgcgg cagataccag gcaaattttt gccattcgcg
    15721 tttcatgatt cgcggcacat ctttcatgat accgaaaatc ccggtatctg gcggtgtagc
    15781 gccagtcaat cgtgcttcca gttgttcagc caataaaccg ttaaacggag cggcaatcca
    15841 gttagcaatc gtggagaaga aatagccaaa cactaacagc acagagatga cagccagagg
    15901 ccacaacaga taactcagcc attgtagcca gtccggaacg taactcatga gagtcgggat
    15961 ccagacatcg agctgtgtaa agagccacca gaatgcgccc cccatcaaca aaatattgac
    16021 cagcagcggt aaaataacga aacgccgaat cccaggttgc gagacgagct tccagccttg
    16081 cgcaaaatag taaaaaccgc tgcgtggggc agatgtgaat gatgaaacca taatcaggat
    16141 gagctccttt tgaccaatcc caggaaaatt ctgcgtattt taccgggtaa ttgcgcaatg
    16201 gacagttagg atatgttcga aaaaacagca aaaagcacga tttcatctat ctttgtgctg
    16261 tgaaagttaa tagtgcactt gcacttgagg taatcggcaa atactcttag tgagtaaatg
    16321 tttgccgtgg tggcaaggtg ttagaacaac agagaatata atgatgcagg atttgcgtct
    16381 gatattaatc attgttggcg cgatcgccat aatcgcttta ctggtacatg gtttctggac
    16441 cagccgtaaa gaacgatctt ctatgttccg cgatcggcca ttaaaacgaa tgaagtcaaa
    16501 acgtgacgac gattattatg acgaggatgt cgaagatgat gagggcgttg gtgaggttcg
    16561 tgttcaccgc gtgaatcatg ccccggctaa cgctcaggag catgaggctg ctcgtccgtc
    16621 gccgcaacac cagtaccaac cgccttatgc gtctgcgcag ccgcgtcaac cggtccagca
    16681 gccgcctgaa gcgcaggtac cgccgcaaca tgctccgcgt ccagcgcagc cggtgcagca
    16741 gcctgcctat cagccgcagc ctgaacagcc gttgcagcag ccagtttcgc cacaggtcgc
    16801 gccagcgccg cagcctgtgc attcagcacc gcaaccggca caacaggctt tccagcctgc
    16861 agaacccgta gcggcaccac agcctgagcc tgtagcggaa ccggctccag ttatggataa
    16921 accgaagcgc aaagaagcgg tgattatcat gaacgtcgcg gcgcatcacg gtagcgagct
    16981 aaacggtgaa ctgcttctta acagcattca acaagcgggc ttcatttttg gcgatatgaa
    17041 tatttaccat cgtcatctta gcccggatgg cagcggcccg gcgttattca gcctggcgaa
    17101 tatggtgaaa ccgggaacct ttgatcctga aatgaaggat ttcactactc cgggtgtcac
    17161 tatctttatg caggtaccgt cttacggtga cgagctgcag aacttcaagc tgatgctgca
    17221 atctgcgcag catattgccg atgaagtggg cggtgtcgtg cttgacgatc agcgccgtat
    17281 gatgactccg cagaaattgc gcgagtacca ggacatcatc cgcgaagtca aagacgccaa
    17341 cgcctgatac acttaaggca aattaactcc tcttcgaacc cccgcttgtc gggggttttt
    17401 agcattgatg gtgcgatatg gaatcaatcg aacaacaact gacagaactg cgaacgacgc
    17461 ttcgccatca tgaatatctt tatcatgtga tggatgcgcc ggaaattccc gacgctgaat
    17521 acgacaggca gatgcgcgaa ctgcgcgagc tggaaactaa acatccagaa ctgattacgc
    17581 ctgattcgcc tacccaacgt gtaggtgctg cgccgctggc ggctttcagc cagatacgcc
    17641 atgaagtacc aatgctgtca ctggataacg tttttgatga agaaagcttt cttgctttca
    17701 acaaacgtgt gcaggaccgt ctgaaaaaca acgagaaagt cacctggtgc tgtgagctga
    17761 agctggatgg ccttgccgtc agtattctgt atgaaaatgg cgttttagtc agtgccgcga
    17821 cccgtggcga tggcaccacc ggggaagata tcacgtctaa tgtgcgtact attcgcgcca
    17881 ttccgctgaa gctgcatgga gagaatatcc cggcgcgtct ggaagtgcgt ggtgaagtgt
    17941 tcctgccgca ggcggggttc gaaaagatta acgaagatgc gcgacgcacg ggcgggaaag
    18001 tttttgctaa cccacgtaat gcggcagctg gttcactgcg tcagcttgat ccgcgtatta
    18061 cagcgaagcg accgctcact tttttctgct atggcgttgg tgttctggaa ggtggcgagc
    18121 tgccggatac tcatcttggc cgtttactgc aatttaaaaa gtgggggttg ccggtcagcg
    18181 atcgggtaac gctttgtgaa tcggcggaag aagtgctggc gttctatcac aaagtggaag
    18241 aagaccgccc gacgctgggc tttgatatcg acggcgtggt gattaaggtc aactcactgg
    18301 aacagcagga gcagcttggc tttgtcgcgc gtgccccgcg ctgggcggta gcgtttaaat
    18361 tcccggcgca ggagcagatg acctttgtgc gtgacgtcga gtttcaggtt gggcgtactg
    18421 gcgcgattac gcctgttgcg cgtctggaac ctgtccatgt tgctggcgtg ctggtgagta
    18481 acgcaacctt acacaatgcg gatgaaatcg aacgtcttgg tttacgcatt ggcgataaag
    18541 tggtgattcg ccgcgctggc gacgtgatcc cgcaggtggt taacgtcgtg ctttctgaac
    18601 gcccggaaga tacccgtgag gttgtattcc cgacgcattg tccggtatgt ggttctgacg
    18661 ttgagcgtgt ggaaggtgaa gcggttgccc gctgtaccgg tggcctgatt tgcggtgcgc
    18721 agcgtaaaga gtcgctgaaa cactttgttt cccgccgtgc gatggatgtt gacggaatgg
    18781 gcgacaaaat catcgatcag ctggttgaaa aagaatatgt ccacactccg gcagatctgt
    18841 tcaaactcac cgcaggcaaa ctgaccggac tggagcgtat ggggccaaaa tcggcacaaa
    18901 acgtggttaa cgcgctggaa aaagcgaaag aaaccacctt tgctcgcttc ctctatgcac
    18961 ttggcatccg tgaagtcggc gaggccaccg cagcaggtct ggcggcatat ttcggcacgc
    19021 tgaaagcgct ggaagccgct tcgattgaag agctgcaaaa ggtgcctgat gttggcattg
    19081 tcgttgcatc ccacgttcac aacttctttg ccgaagaaag caaccgcaat gtcatcagcg
    19141 agctgttggc ggaaggtgtt cactggcctg cgccgatcgt tatcaacgcg gaagagattg
    19201 acagcccgtt tgctggtaaa accgtggtgc ttacgggcag cttaagccag atgtcgcgtg
    19261 atgacgctaa agctcgactg gtcgaactgg gcgcgaaagt cgcgggcagc gtgtcgaaga
    19321 aaaccgatct ggtgattgcg ggtgaagctg caggatctaa actggcgaag gcgcaggaac
    19381 tgggcattga agtcatcgac gaagcggaaa tgctgcgttt gctgggtagc tgagatggaa
    19441 aaagagcagc tgattgaaat ggccaatacg ataatgccgt ttggcaaata caaagggcgt
    19501 cgcttaatcg acctgccgga ggagtatctg ctgtggtttg cccgcaaaga tgagttcccg
    19561 gcagggaagc tcggtgagct aatgcaaatc acgctgctga ttaaaaccga ggggctgacg
    19621 caactggtcc agcccctgaa gcgtccgctt taagctttat cggcgctgct ttcctgctgc
    19681 gcctgtaact gttcggtctg gcgtttgtat cgacgcgcca gcaccgcaca gaccatcaat
    19741 tggatctgat ggaaaatcat caggggcagc accatcatac cgatcaccga tgtggggaac
    19801 agaatgtttg ccatcgggat gccatttgcc agactctttt tcgaaccaca aaagacgata
    19861 gtaatttcat ctgccttatt gaagcccagt cggcgtgcca taaagacgtt aactacaatc
    19921 acgatagcca gaagaacgca gctgaccacc acgataaaca gcaatgatcc ccagccaact
    19981 ttatgccaga taccattaac gacggcttcg ctgaacgctg tataaaccac caacagaatg
    20041 gacgtctggt cagttttcgc aatccatttt ttattgcgcg acacccagtc accaatccac
    20101 ggccgggaaa gatgccccaa cacaaacggc agcagcagtt gcagcataat tttaccgacc
    20161 tgctcaaggc tgccccctgc accgtgaaca ttcatcacca gaccaaccag caatggtgaa
    20221 aggaaaatcc ccagcaggct ggatgctgac gcagaacaaa ccgccgccgc gacgttaccg
    20281 cccgccattg acgtgaaggc gattgcagac tgcacggtag ccgggagagt gcacaagtag
    20341 agaaaaccgg agtagagcat cgggtcgaca tttaccggtt tccaccaggc aaacagtaca
    20401 cccagaatcg gaaacagcac gaaggtgctg cacattaccc acaaatgcag tcgccagtga
    20461 ccaccgccag caataatcgc ctcacgcgac aacttcgcgc cgtgcataaa gaacagcagg
    20521 gcaatagctg cggtggtcag attttcaaag aaggggacga aatcgcctct ggccggaaag
    20581 aaagaggcca gcaacaccac cgtaatcagg gttaaggtga aaggatcgag gatacgaaaa
    20641 agtttcataa agactccaga tgatcgatgg ttctattgtg cttctttcag tttgagaaat
    20701 aaaattgatt tattgcatcc attcatgaag aaaacagatg aattattctt taaaacaatt
    20761 aaaggttttc gtcacagtag cgcaggagaa aagttttagt cgtgcaggag agcgtatcgg
    20821 cttgagccag tcggcagtaa gtcacagtgt gaaggagctg gaaaatcata ctggtgttcg
    20881 cctgctggac agaaccacgc gtgaagtggt gcttacagat gcagggcagc agttggcttt
    20941 gcgtcttgag cgactgctgg atgaacttaa cagcacgttg cgcgataccg ggcgtatggg
    21001 gcaacaactg agcggaaaag ttcgggtcgc tgccagccag accatttccg cgcatcttat
    21061 tccgcaatgc attgctgaaa gccatcgccg ctatccagat attcagtttg tcctgcacga
    21121 tcgtccgcag cagtgggtga tggaaagtat tcgccaggga gatgtcgact ttggcatcgt
    21181 catcgatcca ggcccagtgg gcgatctgca atgtgaagcg attctttccg agcctttctt
    21241 tctgctttgc catcgcgata gcgctttggc cgtggaagat tatgtaccct ggcaggcatt
    21301 acagggagcg aaattggtgt tgcaggatta cgcgtcaggc agccgaccgc tgattgacgc
    21361 agcgctggcg cgcaatggta ttcaggcgaa tattgtgcag gagattggac atccggcgac
    21421 gctgttcccg atggtagccg caggcatcgg catcagtatt cttcccgcac tggcgctacc
    21481 attgcctgaa ggtagcccac tggtggtaaa gcgcattacg ccggttgttg aacgccagtt
    21541 gatgctggtg cgcaggaaga accgttcact ttccaccgcc gctgaagcgt tatgggacgt
    21601 ggtacgagac cagggcaatg cgctaatggc tggtcgggaa ggggatccgt tgtatcagat
    21661 atagatatcg atatgagtcg ttttttcaac aggatttaga agggatacat tcagagacaa
    21721 acgctgctct ttctcctgcg ccttttctgc ctgctgtttc tgcaactgcg ccagttgtgt
    21781 ttccagtagg gtgatttgct gttgaatcaa ttcggcctgt ttttgcttgt cttcagcact
    21841 gccgctagtg tctacaatct ctttaatttg ttgagtgagt ttgatgattt gctgacttat
    21901 ccgcgcgatt tgtgaagcaa catcgctacc ttgtgatgcc ctgttactac tctgtgtttg
    21961 tatggaagga attgaagttg aagagatagt ggccattgtt cagtcctcgt cactgtacga
    22021 ggtaatatcg gtagaagaac aaggaacttg agaagaaaaa tcaatcttac aaatcgcaga
    22081 tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag gattcgaacc
    22141 tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc cgaagtggtg
    22201 ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga
    22261 gctaatcacc cacttctcaa tttcttgcta cacggcggag actacataaa gtagttggtg
    22321 ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga
    22381 gctaatcacc ccccgctgtg tggagttgca ttatagggag agttcaaaat gagtcaacgc
    22441 attttctaaa gaaattgttc gttcgtcgta aatttaagca agatgatcgc aaaacagacc
    22501 gtgttgcgca atttgtcaac gaaaacaata atgcgtaagg tagaaacccg aactacattg
    22561 aggaatcagg cgggagtgat agaatatcgc ccactcaatt tttccaggat ttgccggttg
    22621 tcggcatctt tctaaacgta aggccatttc atgaaaatca aaactcgctt cgcgccaagc
    22681 ccaacaggct atctgcacgt tggcggcgcg cgtactgctc tttactcctg gctttttgca
    22741 cgtaaccacg gcggtgagtt cgtgctgcgt attgaagaca ccgatcttga gcgttccacg
    22801 ccggaagcta tcgaagccat tatggatggc atgaactggc tgagcctaga gtgggatgaa
    22861 ggtccgtact accagaccaa acgttttgat cgctacaacg cggtgatcga tcagatgctg
    22921 gaagagggca ctgcttataa atgctattgc tctaaagagc gcctggaagc gctgcgcgaa
    22981 gagcaaatgg cgaaaggtga gaagccgcgt tatgacggtc gctgccgcca cagccatgag
    23041 catcacgctg atgatgaacc gtgcgttgta cgttttgcta acccgcagga aggttctgtt
    23101 gtttttgacg atcagatccg tggtccgatc gagttcagca accaggaact ggacgatctt
    23161 attatccgcc gtaccgatgg ttccccaacc tataacttct gtgtggttgt cgatgactgg
    23221 gatatggaaa tcacccacgt tatccgtggc gaagaccata tcaacaacac gccacgccag
    23281 atcaacattc ttaaggccct gaaagcgccg gtgccggttt acgcgcacgt ttctatgatc
    23341 aacggcgatg acggtaaaaa actgtccaaa cgtcacgggg cggtcagcgt aatgcagtat
    23401 cgtgatgacg gttatttgcc agaagcactg ctgaactatc tggtgcgtct gggctggtcc
    23461 cacggcgatc aggaaatctt cactcgtgaa gagatgatca aatacttcac cctgaatgcc
    23521 gtcagcaaat ctgccagtgc gttcaacacc gacaagctgc tgtggctgaa ccatcactac
    23581 attaacgcgc tgccgccgga gtatgttgct actcacttac agtggcacat tgagcaggaa
    23641 aatatcgata cccgtaacgg cccgcagctg gctgatctgg tgaaactgct gggcgaacgc
    23701 tgcaagacgc tgaaagagat ggcacagagc tgccgttatt tctacgaaga ttttgctgag
    23761 ttcgatgccg acgccgcgaa aaaacatctg cgtccggtag cgcgtcagcc gctggaagtg
    23821 gttcgtgaca aactggccgc gattactgac tggaccgctg aaaacgttca tcacgctatt
    23881 caggcgacgg cggatgagct ggaagtgggt atgggtaaag ttggtatgcc gctgcgtgtc
    23941 gccgtaaccg gtgcggggca gtctccggca ctggatgtta ccgttcacgc aattggtagg
    24001 acccgcagta tcgagcgtat caacaaagcg ctggctttta ttgctgaacg cgaaaaccag
    24061 cagtaattag cgcataaaag ataaacggca ggagataata tcctgccgtt ttttatttat
    24121 gctgattcgt caatatcgag acgggcgagg aaattgcgaa ttccttcacg tgacaaaaat
    24181 tgcgcgactt tttcctgctc aatgtctgtc atattatcta tcgcgttgat gatttgtcga
    24241 tacgcagggg cgcgtgctcc aggagcgtat tcagcaattc tctcttcagc ttccatcaac
    24301 attggtgtgt tatggaaggc tggcgtgttc tgaataaact cgcaaacttg cgtatcgacc
    24361 agaataagtc tggcacgacc gcctttcacg ccgggaaatt tttccgtttt ccagcctttt
    24421 tctctgatcc aacggttaac agtttgttta gccacaccga ggcattcagc cagttcttcg
    24481 gtggtcattt tattgcgtaa tcttttcata ttatttgcta tcgcgaatcc ctaagcgttg
    24541 taacaatccg ataatccctt cccgcagaag caaggatgta aactgttttt gttcaactgg
    24601 cgtcatctct ttcgccagtg tcaccagcaa tacttcaagc ggcgcatcac cagaaagggc
    24661 aggcgcttct ccctgacctt ctggacgttc agcattgcga atatattcac gaacctgttc
    24721 attgacgtga accagtcggg ctttgccacc ctggacgcca ggttttggtg acgttgtcca
    24781 gccttccttg cgtacccatt tattaatggt ctggcggcta tagccggtca gtctggcaag
    24841 ttcatctggt gtcatcctct ccttgaacat aacaattccc tgaatgtatg tgggtctatt
    24901 agtagttcat tttataacac cactttacag gaaaccgtga catgaataac tactgaatgc
    24961 gagcggtttt ccaaaattgt tcatttgcct gatttttcag cgattgaaac tacacgttgc
    25021 aatttgccgt tgacacattc gggcggaatt catatgatgc cgcccgtcaa ctcgacaagc
    25081 tttacgtgac ggggctatag ctcagctggg agagcgcttg catggcatgc aagaggtcag
    25141 cggttcgatc ccgcttagct ccaccaaatt ttgcacccag caaatttggt acgtaaacgc
    25201 atcgtggggc tatagctcag ctgggagagc gcttgcatgg catgcaagag gtcagcggtt
    25261 cgatcccgct tagctccacc aaatttccaa ccctcgctgc aaagcggggg ttttttgtct
    25321 ctgctttttg ccgcttttgt aatacagtct acgtccgggt tagtgccgcc aggtgaaagc
    25381 atcattggat gaaaaatcgg caacaggctg gccccctgtt tgcttcgcga tgcgaataaa
    25441 cttattattt gtgtgcctga aaaccccgat cagtgagagt agtgtactca tgtttgtgga
    25501 gcataacctg ataaaaaata tcaagatatt cacactagcg tttacgctca ccgtggtact
    25561 tattcagcta tcccgtttta tttcgccact tgccattatc cattccagtt atatctttct
    25621 ggcgtggatg ccactgtgcg taatgctgtc aatcttgttt atctttggct ggcgaggtgt
    25681 cgttcccgtt ttatgcggga tgttttgcac caatctgtgg aactttcatc tctctttttt
    25741 acagactgcg gtcatgctcg gtagccagac gtttgtcgtg ttgtgtgcct gcgcaatatt
    25801 acgctggcag ctggggacgc gttggcgtta tggattgacc agccgatatg tctggcaacg
    25861 tctgttctgg cttggtttgg tgacgccgat cggcatcaaa tgcagcatgt atcttgtggg
    25921 gagcttcttt gatttcccgc taaagatatc cacctttttc ggcgatgcgg atgccatttt
    25981 cacggtcgtt gatttgctaa gccttttcac cgctgtgctg atttacaaca tgcttttcta
    26041 ctatctcact cgcatgattg taagtcccca ctttgcgcag atattgtggc gcagggatat
    26101 cgcgccgtcg ttgggcaaag agaaacgcgc atttacctta agctggctgg cagcgttaag
    26161 cgtgcttcta cttctgttgt gcacacctta tgaaaacgac tttattgccg gttacctggt
    26221 acccgttttc ttcatcatct ttaccctcgg ggtcggtaag cttcgctatc cgtttttaaa
    26281 tctcacctgg gctgtttcaa cgctttgcct tctgaattac aaccagaact ttttgcaagg
    26341 ggtggaaacc gaatattcgc tggcatttat tcttgcggtg ctgatttcct ttagcgtttg
    26401 cctgctctat atggtgcgca tttatcatcg cagtgaatgg cttaatcgcc gctggcattt
    26461 gcaggcgctg accgatccgt taacgctcct gcccaacttc cgtgcgttgg aacaagcgcc
    26521 ggagcaagag gcgggcaaga gtttttgctg cctgcgcatt gataatcttg agtttatgag
    26581 tcgtcattac ggcttaatga tgcgcgttca ctgtatccgc tcaatttgcc gtacgctgct
    26641 gccgttgatg caggaaaacg aaaagttgta tcaattgccg ggtagtgaac tgctgttagt
    26701 gctgagcggg ccggaaacgg aagggcgact ccagcatatg gttaacatcc tgaatagtcg
    26761 gcaaattcac tggaacaata ccgggctgga tatgggctat ggtgctgcct gggggcgttt
    26821 tgatggaaat caggaaaccc tgcaaccctt gttggggcag ttaagctggc tggcggagca
    26881 atcctgcgca catcatcatg tgctagcgct ggatagcaga gaggagatgg tttccgggca
    26941 gaccactaaa caggtgctat tgctgaatac cattcgcacg gcgttatatc agggtgattt
    27001 gctgctctac gcccagccaa ttcgcaacaa agagggtgaa ggttatgatg agatcctcgc
    27061 gcgactgaaa tatgacggcg gcattatgac cccggataag tttctgcccc ttattgctca
    27121 gtttaacctt agcgcgcgtt ttgatttgca agtgctggaa tccttgttga agtggctggc
    27181 aacacaccct tgcgacaaaa aaggaccgcg cttttcagtc aatttaatgc cgctcacgct
    27241 gctgcaaaag aatattgccg ggcggattat tcgtctgttt aagcgttatc acatctcccc
    27301 gcaggcggtc attcttgaga tcaccgagga gcaggcgttt tctaacgcag aaagcagcat
    27361 gtacaacatc gagcagctgc ataagtttgg tttctggatt gcgattgatg actttggcac
    27421 cggctatgcc aactacgaac ggttaaagcg tttgcaggct gatatcatca aaattgatgg
    27481 cgtctttgtg aaagatattg tcacgaacac gctggatgcg atgattgtga gatcaattac
    27541 cgatctggcg aaagcgaagt cattgagtgt ggtcgcggag tttgtcgaga cgcaacagca
    27601 gcaggcgcta ttgcataagc tcggggtgca atatctgcaa gggtatttga ttggtcgccc
    27661 gcagccatta gctgattaac aggcgtaaaa aaaccgggga attatcc
//
LOCUS       DAJTMK010000037        27506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTMK010000037 DAJTMK010000000
VERSION     DAJTMK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27506
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.038"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..589
                     /locus_tag="OO135_001362"
     CDS             <1..589
                     /locus_tag="OO135_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5397273.1"
                     /translation="RNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMY
                     CTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGE
                     RISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPP
                     EEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
     gene            complement(631..1206)
                     /locus_tag="OO135_001363"
     CDS             complement(631..1206)
                     /locus_tag="OO135_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5397274.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1210..1776)
                     /locus_tag="OO135_001364"
     CDS             complement(1210..1776)
                     /locus_tag="OO135_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5397275.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            complement(2037..2150)
                     /locus_tag="OO135_001365"
     CDS             complement(2037..2150)
                     /locus_tag="OO135_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5397276.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            complement(2198..3316)
                     /gene="solA"
                     /locus_tag="OO135_001366"
     CDS             complement(2198..3316)
                     /gene="solA"
                     /locus_tag="OO135_001366"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5397277.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRSGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            complement(3431..3685)
                     /gene="bssS"
                     /locus_tag="OO135_001367"
     CDS             complement(3431..3685)
                     /gene="bssS"
                     /locus_tag="OO135_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5397278.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            complement(3974..4219)
                     /gene="dinI"
                     /locus_tag="OO135_001368"
     CDS             complement(3974..4219)
                     /gene="dinI"
                     /locus_tag="OO135_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5397279.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            complement(4293..5339)
                     /gene="pyrC"
                     /locus_tag="OO135_001369"
     CDS             complement(4293..5339)
                     /gene="pyrC"
                     /locus_tag="OO135_001369"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5397280.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            complement(5445..6005)
                     /locus_tag="OO135_001370"
     CDS             complement(5445..6005)
                     /locus_tag="OO135_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5397281.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            complement(6139..6786)
                     /gene="grxB"
                     /locus_tag="OO135_001371"
     CDS             complement(6139..6786)
                     /gene="grxB"
                     /locus_tag="OO135_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5397282.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            complement(6850..8058)
                     /gene="mdtH"
                     /locus_tag="OO135_001372"
     CDS             complement(6850..8058)
                     /gene="mdtH"
                     /locus_tag="OO135_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5397283.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIESIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            8293..8877
                     /gene="rimJ"
                     /locus_tag="OO135_001373"
     CDS             8293..8877
                     /gene="rimJ"
                     /locus_tag="OO135_001373"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5397284.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFTNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALAAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTLDWTPGR"
     gene            8888..9535
                     /locus_tag="OO135_001374"
     CDS             8888..9535
                     /locus_tag="OO135_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5397285.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTQEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            9537..10403
                     /locus_tag="OO135_001375"
     CDS             9537..10403
                     /locus_tag="OO135_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5397286.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            10570..12105
                     /gene="murJ"
                     /locus_tag="OO135_001376"
     CDS             10570..12105
                     /gene="murJ"
                     /locus_tag="OO135_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5397287.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLM
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSCVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(12145..12561)
                     /gene="flgN"
                     /locus_tag="OO135_001377"
     CDS             complement(12145..12561)
                     /gene="flgN"
                     /locus_tag="OO135_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5397288.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(12566..12865)
                     /gene="flgM"
                     /locus_tag="OO135_001378"
     CDS             complement(12566..12865)
                     /gene="flgM"
                     /locus_tag="OO135_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5397289.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSVT
                     LSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQDLQSN"
     gene            complement(12941..13600)
                     /gene="flgA"
                     /locus_tag="OO135_001379"
     CDS             complement(12941..13600)
                     /gene="flgA"
                     /locus_tag="OO135_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5397290.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGLVVSGVVDADGNILIN
                     L"
     gene            13756..14172
                     /gene="flgB"
                     /locus_tag="OO135_001380"
     CDS             13756..14172
                     /gene="flgB"
                     /locus_tag="OO135_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5397291.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            14176..14580
                     /gene="flgC"
                     /locus_tag="OO135_001381"
     CDS             14176..14580
                     /gene="flgC"
                     /locus_tag="OO135_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR5397292.1"
                     /translation="MALLNIFDIAGSALTAQFQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            14592..15287
                     /gene="flgD"
                     /locus_tag="OO135_001382"
                     /pseudo
     CDS             14592..15287
                     /gene="flgD"
                     /locus_tag="OO135_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            15312..16520
                     /gene="flgE"
                     /locus_tag="OO135_001383"
     CDS             15312..16520
                     /gene="flgE"
                     /locus_tag="OO135_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5397293.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVNGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFATLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILKTLVNLR"
     gene            16540..17295
                     /gene="flgF"
                     /locus_tag="OO135_001384"
     CDS             16540..17295
                     /gene="flgF"
                     /locus_tag="OO135_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR5397294.1"
                     /translation="MDHAIYTAMGAASQTRNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGC
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            17433..18215
                     /gene="flgG"
                     /locus_tag="OO135_001385"
     CDS             17433..18215
                     /gene="flgG"
                     /locus_tag="OO135_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5397295.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            18268..18966
                     /gene="flgH"
                     /locus_tag="OO135_001386"
     CDS             18268..18966
                     /gene="flgH"
                     /locus_tag="OO135_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5397296.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNVRADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            18978..20075
                     /gene="flgI"
                     /locus_tag="OO135_001387"
     CDS             18978..20075
                     /gene="flgI"
                     /locus_tag="OO135_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5397297.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            20075..21016
                     /gene="flgJ"
                     /locus_tag="OO135_001388"
                     /pseudo
     CDS             20075..21016
                     /gene="flgJ"
                     /locus_tag="OO135_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            21082..22725
                     /gene="flgK"
                     /locus_tag="OO135_001389"
     CDS             21082..22725
                     /gene="flgK"
                     /locus_tag="OO135_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR5397298.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            22737..23690
                     /gene="flgL"
                     /locus_tag="OO135_001390"
     CDS             22737..23690
                     /gene="flgL"
                     /locus_tag="OO135_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5397299.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(23886..27071)
                     /gene="rne"
                     /locus_tag="OO135_001391"
     CDS             complement(23886..27071)
                     /gene="rne"
                     /locus_tag="OO135_001391"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5397300.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRYERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
BASE COUNT         6668 a         6939 c         7320 g         6579 t
ORIGIN      
        1 gcgtaaccac tatgagtatg aagtggggca ctttgaaaac gcgctcgaaa ttccggcaga
       61 taccttccgt gagcagctgc caaaagcagt cgagatgatg caggcacata aagataaaaa
      121 aatcgtcatg tactgcaccg gcggcattcg ttgtgaaaag gccagtgctt ggatgaaaca
      181 taacggattc aacaaagtct ggcatatcga gggcggaatt attgaatacg cccgtaaggc
      241 gcgcgagcag ggcttgccgg tgcgttttat tggcaaaaat tttgtttttg acgagcggat
      301 gggcgaacgt atatcggatg agattatcgc gcattgccac cagtgcggcg cgccgtgcga
      361 cagccatacc aactgtaaaa atgatggctg ccacctgctg tttattcagt gtccagtatg
      421 cgcggaaaaa tacaaaggtt gttgtagtga gatttgctgc gaagaaagcg cgttaccgcc
      481 agaagaacag cgacgccgtc gggcaggacg tgaaaatggc aataagatct ttaataagtc
      541 tcgtggacgt ctgaatacaa cactgggcat tcctgatcca acagaataaa tatcattgcc
      601 ggatgcgtgc catccggcaa catttcacgc ttacttctgc tgtacgcctt ccactgaaat
      661 aatcagatcc acttcctgag aagctggacc taaatctgtc ttgatattga agtccttgag
      721 cttaattttg ccttcggcct cgaagcctgc acgtttacca ccccatgggt cgtcgccctg
      781 accaattaat ttcgcttcca gcgtgacggg tttggttacg ccattcagcg tcagatcgcc
      841 ggtaatatcc agttcgtcac cgtctttctt cacgctggtg gaggtgaatg ttgcctgtgg
      901 atattttgcg gtattgagga aatctgcact gcgaagatgt ttatcgcgtt cggcgtgatt
      961 agtatcgacg ctggtggtgt taattgtcac attcacttta tcggcagccg gatttttttc
     1021 gtcaaaggta aaagtaccgt cgaaatcttt aaaggtgccg tataaccagc tatagccaag
     1081 gtgctggatg cggaaattaa caaaggcgtg ctgaccttct ttgtcaattt tgtaatcggc
     1141 ggcaaccgct gaaccggcag agaacatcag ggacgcgaag gttaaaccaa gcaggctttt
     1201 tttcattttt tatactccat agtcagatga cgactttccc agcatgcgct tcagagtgtc
     1261 gtctttatcg atgaaatgat gcttcagggc cataaatccg tgcataacgg acagtactac
     1321 gacgctccac gcgagccaaa aatgcagggc accggcaaag tctgcctgtg cgccagcgtc
     1381 ggcaagggtc gcggggacgt caaaccagcc aaaaacgctg atcggtttac catcggcagt
     1441 tgagatcaga tagccgctga tgccgatggc gaacagcaat aggtacaatg cgagatgtcc
     1501 agcctttgcg gcaagacgag tcatgggcga ataactcggc agcggacccg gcggtggaga
     1561 tatgacacgc cacagaacac gaataaccag ccccatcatt aacaaaatac cgatgctttt
     1621 atgcagttcg ggtgctttgt gataccagcc atcgtaataa ctgagcgtga ccatccacag
     1681 acctaaagca aacatgccgt agacaataat tgcgcttagc caatggaagg ccgcagatat
     1741 aacaccatag cgttcagggg tatttgtgaa tgacataagt gcatccaata cgaatttgcg
     1801 agagaaagaa aatggcgtgt cggagaatga attgcaacac aaaaataaca aattgaatga
     1861 tatttatttt tatttcaata attttgaatt aataatgaaa gaatcttcat aagtttagtg
     1921 ggttcagcat cagagatgtc tggtgagtgc tgtagaagca ggaataatct cattcaattt
     1981 ttcacgctaa atgaaaggga gattaaatat ttgtcagttt atgtcagtgc ggaaaactaa
     2041 aaataaaaca catagatcag atccataatt gccaacaatc cccagagaaa aagataaagc
     2101 atcagatgct cgcgaatatt attgatgaga tagtgcaaca gccgacgcat taaatactcc
     2161 tgtaaaaacg gcctcattct ggaggccgta atgatgatta ttggaagcgg gaaagcctga
     2221 atggcgtcaa atcaaaatcg ctttttttgt cttgcgcaaa atcggcagct atttccccta
     2281 aaactgacgc aaatttaaaa ccgtgcccac tcaggccggt aatgagcagt gtattatcgt
     2341 ggccgggtag ggtatcgata ataaaatctt cgtcaggcga attatcatag gtgcaggcag
     2401 cgccgtacag gcagcaaccg atacccggca atacattgcg caagaacggg aaggcttccg
     2461 aaccatcgct gaccacttcc gcaaacggaa cacgttcatc cgctgaatgg ataacctggc
     2521 ctccgttatg tttgccaatc ttcaacgcgt cgttttctgc cggaaaacca taatattgat
     2581 cgccattggg cagttcaccg gtaaacgccg ggaatttatt cttcacgcta tagcggccat
     2641 cggcctgata ccaggcaaat actttacgta caggctggac aggcagctcc gggagcaggt
     2701 cttttaccca tgttcccgcg cagacaatcg cttttttcgc ctgatactca ccgtcaacgg
     2761 tttcaatagt tacgccatca tcgtcatgac gaattgcggt gaccgggcag ttgaacagtt
     2821 gcgcacagcc cgcttccttc gccagttgga tccaggtttt aatcgccagt tcgctgcgca
     2881 aaaaaccgga atcagtctca aataagccga tgtagttgtc cgggacgcgt atttctggcc
     2941 agcgggccat aatcccttgc gcatcgagct tttcaacgtt gagttgccac tgttcggcgc
     3001 tgtgggcgac gttggcgaga aatgcggagt cagccgggcc gaggttaatg acaccagagc
     3061 gtacaaaaat gggatcatct tcgttgtggc gggaaagttc atcccacagc gtttgcgcgc
     3121 ggaggaccaa cgggacatac ttttcgcctt caccataagc atggcgaatt aatcgcgtat
     3181 cgccgtggtg gctgccgtgt tgatgcggtg gcatatgggc gtcggtcatt agcacgttta
     3241 aaccggagcg ggttgcataa tacccggcgg cagcgcctac ggaaccgctg ccaataatga
     3301 tgagatcgta tttcattttt ttctctctgc tctcatggtt taagcagagt aattaactga
     3361 atgcagatat acaacccaga aatggtaaag gcaccggtga ggtgcctttt gggtggatgg
     3421 tcatgtcatg ttaatgacgc cgatactcgt ttaccgggaa atcaccggat tcaattttgg
     3481 cgattcctgc ttctaatatc gaaataaatt gtctggcaac atcagtggtt aaccagagcg
     3541 tctgaccaac ttcagtccct tcctgctcgg acttatttgg ggtctggtag tgcaaacgca
     3601 acatcagcgc atcatagcta tcaacggtgc tgatgtccca ccctacgagc ggatgagtct
     3661 gaatgacttc attatttttt tccatcatgg ccccctaatt cgtgttacta gacaacgtta
     3721 ttcgaggttc aatgcgtttt tatctgaagc aacttcagta taccaataag taagggtatt
     3781 caccgaattt ttaaaggggg gaacagataa atttctgctt ttaagattta tatgaacaat
     3841 aaaacagcat gtcattcata ttttttagca tattgtgcaa ttattttgag gaagtgtaga
     3901 aattttgtac tcaaaatttg taagtaaaat aaaaaagccg gggcgacccg gcaaaaacaa
     3961 aaatcactgc atattattcg ctgacaaacc agtcatcggc gctttcccac gtttcctgga
     4021 gaatttcgct aatgcgctgt ttatcttctt ttgttacgcc aataaccgat aaattattcg
     4081 ctgcggcata acgtaccgat acgtggcctt cattatcagg aaacgcatac tgaatacggc
     4141 gggaaagttc gccagccagg gcgtcaatag ccccagctgg caatggagaa gttttcgcta
     4201 tggtgacttc aattcgcata atagccccct gttgaatata ctggttattt atacaggcaa
     4261 aataacctaa tgacaacagg aagctacgat ttttattgtt taacggacca gcgtaccgtt
     4321 tccccggcga ggaatggcac cagggtgtca tcagtcagtg cgatgctttc agcaacctgt
     4381 tgctcttcac gtaccagttc gatgaatgtg tcgttgaccg gcaagccata gaactgcggg
     4441 ccgtttacag aacagaatgc ttcaaagtgc tgcagagcat tcatctcttc aaagacggta
     4501 gcgtaactgc ccagcgcggt tggggcgttg aagcagcccg cgcagccgca actgctctct
     4561 ttgcgatgac gtgcatgtgg cgcagaatcc gtaccgagga atactcgatt aaaaccgctg
     4621 gcgaccagtt cacgcaatgc ctgttggtga atattgcgtt tgaggatggg taaacaatac
     4681 aggtgcggac gcacgccacc aaccagcata tggttgcggt taaacatcag atgctgcgga
     4741 gtgatggtgg cagccagccg ttcatttccg tcacggacat agtcggcagc atctttggtg
     4801 gtgatgtgct caaaaacgac tttcagcgca gtcaggcgct ggcgcagagg ttccatcacg
     4861 ctttctataa agtgcgcttc acgatcaaaa atgtcgatat ctgcatgtgt cacttcacca
     4921 tgcaccagta gcggcatacc gattttttcc atgcgctcaa gtaccggcat gattgcgtca
     4981 attgacgtca cgccgtggct ggagttagtg gttgcgtttg ccggataaag ttttgcagcg
     5041 gtgaacacgc cttcgttaaa tccgcgctcc agctcattag gatccagcga atctgttaaa
     5101 taacaggtca tcaatggggt gaaattgtgc ccggcaggta cggcatcaag aatacgctgg
     5161 cgatacgcca cggcagcctc aacagtggtc acgggcggag ccagattggg cattacaata
     5221 gcccgtccat aaatttcgct ggtatacggc acgacagttt ttaacatgtc gccatcgcgg
     5281 aggtgaaggt gccagtcgtc tgggcggcgg atctttaata cctgggatgg tgcagtcatt
     5341 aatctctatg ctccggctga agggatgttt ttgccggaca caaaggataa gcggaaacgt
     5401 tttcctttgc acgaaaaata aagggcgcga atgcgcccta gtcattaatc cgtaaacgga
     5461 atgacaattt cgcctggctt cacttcaatg cctttcgcca gttttttcgc cattgcttcg
     5521 ccctgactgc catcttcgcg caggacgtaa gcaggttgct ggttaaagta attgcgtaat
     5581 gcctggttca aatagggaag caacgtttgc atcaccgttt gcattttttc cggttgtacc
     5641 gtcgcatcga ccacttccat ctctttcagg aagatcgcac ctttttcttt atcaaacacg
     5701 ggcagcgctt tcagcttcag tttcatggtc gctttctgac taccgaacag ggagttcatg
     5761 tccagattgg cgtctccggt tagggtaacc ttattcggct cttcgcgacc aatttggctg
     5821 gtcaagtttg tcagaacaat atgggcgtca gccacgccgg gtaaaccgat atcttttgag
     5881 aaattattat gtttcgcaag cgactggtta atttcttgtt cggtgatggt gtattgggtg
     5941 agttgattac agccaacgag caggccactg acgatcaatg cagcggcaaa taaaaacttg
     6001 ttcatggtag tcctcgacat gaaatctgcg tcaatatcct gacacaacgc agcatgtgtc
     6061 accagcgata aactcgccag cagaaaaaac tgaaaacggc ggcaacccgc gaatacaggc
     6121 tgccgcggcg ggtcaggatt aaatcgccat tgatgataac aaattgattt gtgtctgttt
     6181 cgccatatta tcgcggtaat cagcaacgcg gcttggccag ttgattccgg ctaccagcgt
     6241 cagattacgc agtagcggaa atagctgaat atcatcttcc gaaagttcgc cattcacggc
     6301 gttcggtttg acgatcagtt tgtccagcgc acgcaaatca tcgctgatat tcttaatcag
     6361 accgtcagag tgggccagca ggtcggcaaa attacccgcg ctggcctctt tcttgtcgac
     6421 gaaatatttg cgcgcggcgg gagtagaaaa ctcatcaaat gccgatttgg caaaacgcgg
     6481 caacagcagt ttgttggcgt agccattgac cttgcgcagc cactcttcaa tggcagggga
     6541 acgtttgccg gtcagtaacg gtttgccgtc gagtttatcg acatagtgaa cgatatccat
     6601 gctttctggc atatagcggc tgtcatcttt ttgcagaatg ggaacctgtt tttgaccgac
     6661 catccgggtg ggtgtttctg cgtcgtcgtt gagcagaaca tgtaattcga cggggatatt
     6721 tttcaggccg aaaatcatgc gggctttgag gcagtaaggg cagtgatcgt aaatgtatag
     6781 cttcacgtga ctcctccatt tggctgtcag ttcactttca gtatggagga gtcagggggg
     6841 agtatcaaat caggcgtcgc gttccagcaa acgacgcgcg gcgcgtttct gactaaactg
     6901 ccaacccagc gcaaggaaag tgaagatgcc aataattccc agcatcatcc acggaagctc
     6961 tggctggtgc gccgatttgc ccaggtcaaa cagccagcca ccaccgatat aaccaatagc
     7021 gccgccaatc gccagaccca gacggctaaa ccccatatag ctgccgcgag ctcttgcgtc
     7081 cgccagcgaa gcacttaagg tttcacgcgc aggctcggca atgatcgact cgatataaaa
     7141 cagacaaatc agggtgaaaa gttgttgcag gccgctgacc atgcccaccg gcatcatgct
     7201 taatgacatt atcaacagcc cagccatcaa ccggtgttcc agacgaaaat gcttttcact
     7261 ccagcgggcg atagggtaga gcaacgttaa cgacagacac gcttcaatgg catacatcca
     7321 tttaacggca gagggcgcgc cagccacgtc gttgaccata attggcagca tcagcatcac
     7381 ttgtacagcc agcatgtagt aacccgccag cgtcaggaca taggtgacaa aacgcttgtc
     7441 acgcatcacg cgggtcatgc cttcgcgaac gggcgtgcgt acggtggaga gtttccatgc
     7501 cggtaacaac cacgcattga acgccgcaca cagcacaaac aaaacggccc ctgtggcgca
     7561 gaccaggcga aagtcgtatt gcaacagcca gctccccaac aatgcgccaa tgaccgcacc
     7621 ggcactgtcc tgcatcatca acagcgagaa aaaacgacca cgttgttgtg gacggattag
     7681 tttcaccacc agcgccgaac gcggcggatc aaacaacgtg ccgccgagtc ccgagagcag
     7741 gcatgaaaac cacaatagcc acggttcgtg ggcgataccc attgtggcga atccggcggc
     7801 gcgcatcagc ataccggtaa caatcatcgg tttggcacca aagcggtcgg caattgcgcc
     7861 gccgaaaata cccagacctt gctgaataaa ctggcgtaga ccgagagcaa taccgaccat
     7921 gacggcggcc cagcccattt gatcaacgaa gcggatagag atcagcggga agacaacaaa
     7981 gaaccccagc acgaccagca tattatcgat gagcaggaaa tatttaccca ggttcctcgc
     8041 ctgcgatacg cgggacattt cccctcccgg gaaataaaag atgagcactt tctattctgc
     8101 attgccgcta agacttttcc catcacttcg gggacaatat tttttatcaa aacgtcgctt
     8161 tgatagagag tttttatcaa atatgtgaat aatgcagaaa atggcatttt ggacttttca
     8221 cagggtctgg ttgcgcaggt atagtaatac ttacagcgta ttaaagacgt tacgggaagg
     8281 agtaggtata gaatgtttgg ctatcgcagt aacgtgccaa aagtgcgctt aaccacagac
     8341 cgactggtcg tgcgtctggt gcatgatcgt gatgcctggc gtcttgcgga ttattacgca
     8401 gagaatcgcc atttcctcaa gccctgggag ccagtgcgcg acgaaagcca ctgttatcca
     8461 tcaggctggc aggccaggct ggggatgatt aacgaatttc ataaacaagg ttcagctttc
     8521 tactttggct tattcgaccc ggacgaaaaa gagattattg gcgttgccaa ttttaccaat
     8581 gttgttcgtg gctcttttca tgcctgctat ctcggttatt cgattgggca aaaatggcag
     8641 ggcaaagggc tcatgtttga agccctggcc gcagccattc gttatatgca gcgcacccaa
     8701 catattcatc gcattatggc taattatatg ccgcacaata aacgcagcgg tgatttactg
     8761 gcgcgactgg gttttgaaaa agaaggctat gcgaaagact atctgttgat tgatggacaa
     8821 tggcgcgatc acgtactgac ggcattaact accctagact ggacgcccgg ccgctaagga
     8881 gaatctcatg aaatatcaac ttacagccct tgaagctcgc gtgattggtt gcttgctgga
     8941 aaagcaggtg accacgcagg agcaataccc gctctcagtc aatggtgtag tcacggcctg
     9001 taatcagaaa acgaaccgtg aaccggtcat gaatctgagt gaatctgaag tgcaggagca
     9061 actggataat ctggtcaaac gccattatct gcgcacggtg agtggctttg gtaatcgggt
     9121 caccaaatat gaacaacgtt tttgtaattc agaatttggc gatctgaaac tgagcgcagc
     9181 ggaagtggcg ttaatcacca cgttgttatt gcgtggtgcc cagacgccgg gtgaactgcg
     9241 cagccgcgcc gcgcgaatgt atgaattcag cgatatggcg gaagtggagt cgacgctgga
     9301 acaactggca aatcgcgaag atggtccttt tgtggtgcgt ctggcccgcg aaccgggtaa
     9361 acgcgaaagc cgctacatgc atcttttcag tggtgaggta gaggatcagc cggcggtaac
     9421 ggatatgtcg aacgcggttg atggtgattt acaggcccgc gtcgaagccc tggaaatcga
     9481 agtggcagaa ctgaaacagc gtcttgattc gttgctggcc catctgggag attaaagtga
     9541 aaaaattacg tatcggcgtt gtgggattag gcggcattgc gcaaaaagcg tggttaccgg
     9601 tgctggcggc agcgtctgac tggatgttac aaggagcctg gtcgcctacg cgcgcgaaag
     9661 ccctgccaat ttgtgaaagc tggcgcattc cttatgccga ttcgttatcc agccttgccg
     9721 ccagttgcga tgcggttttt gtgcattcca gcaccgccag ccactttgac gtggtcagta
     9781 cgttactcaa tgcgggtgta catgtctgtg tcgataaacc gctggcagaa aatctgcgcg
     9841 atgctgaacg gctggtggaa ctggcggcgc ggaaaaaact gacgttgatg gtcggtttta
     9901 accgtcgttt cgcaccactc tacggtgagt taaaaacgca actcgctacc gcatcttcgc
     9961 taagaatgga taaacatcgt agcaatagtg tcgggccaca cgatctttat ttcacgttgc
    10021 tggatgatta tctgcatgtg gtggataccg cgctgtggtt gtcgggcggc aaagcctctc
    10081 tggatggcgg tacgctactg actaacgacg ctggcgaaat gctgtttgcc gagcaccatt
    10141 tttcggccgg tcctttgcag atcaccacct gtatgcatcg ccgtgccgga agtcagcgtg
    10201 aaaccgtgca ggccgtgact gacggtgcgc tcatcgacat tacggatatg cgcgaatggc
    10261 gtgaggagcg cgggcagggc gtagtgcata aaccgattcc tggttggcag agcactcttg
    10321 aacaacgtgg atttgtcggc tgtgcgcggc acttcattga atgtgtgcaa aatcagacag
    10381 ttccgcaaac cgccggcgaa taggccgtgc tggcgcaacg tatcgttgac aagatctggc
    10441 gcgatgcgat gagtgaataa accctgtaac atctggcggt agcaattcac cataatccag
    10501 gtagactatt cgcctctttc agcgcctgtc ttgcaggcgt tttgcccgtt ggtctggatt
    10561 agaacaccga tgaatttatt aaaatcgctg gccgccgtca gctcgatgac catgttttcg
    10621 cgtgtgcttg gcttcgcacg agacgcaatt gtcgccagaa tctttggcgc agggatggca
    10681 accgacgcct ttttcgtcgc ttttaaactt cctaacttgt tacgccgtat ctttgccgaa
    10741 ggggcatttt cccaggcatt tgtaccgatt ctggcggaat ataaaagtaa gcagggtgaa
    10801 gacgccacgc gggtctttgt ctcttatgtt tctggcctgc tgacacttgc gctggcggtt
    10861 gtgacggtcg ctggcatgct cgccgcaccg tgggtgatca tggtgaccgc gccaggcttc
    10921 gctgacacag ctgacaaatt tgccctgacc agccagctac taaagattac ctttccctat
    10981 atcttgctga tctccctggc gtcgctggtg ggagcgattc tgaatacgtg gaaccgcttc
    11041 tcgattccgg cgtttgcgcc aacactgctt aacatcagca tgattggttt cgcgctgttt
    11101 gccgcaccgt actttaaccc accggtgctg gcgctggcgt gggctgttac ggtcggcggc
    11161 atcctgcagc tggtgtatca gctaccgcac ctgaagaaga tcggcatgct ggtcctgccg
    11221 cgcattaact tccacgatgc cggagcaatg cgcgtggtga aacagatggg accggcgatc
    11281 cttggcgtct ctgtgagcca gatctcctta atcatcaaca ccatttttgc ctcgtttctt
    11341 gcttccggtt cggtgtcttg gatgtattac gccgaccgct taatggagtt tccgtccggt
    11401 gtgctgggcg tggcgcttgg caccattttg ctgccgtcgc tgtcgaaaag ttttgccagt
    11461 ggcaatcatg atgaatacaa ccgtttgatg gactgggggt tgcgtctttg tttcctgttg
    11521 gcgctgccga gtgcggttgc gttgggcatt ctttcaggtc cgttaaccgt ttcgctgttc
    11581 cagtacggta aatttaccgc gtttgatgcg ctgatgaccc agcgggcgtt aattgcctac
    11641 tcggtgggtt tgattggcct gattgtagtg aaagtgttgg ctcctggctt ttattcccgc
    11701 caggacatta aaacgccagt gaaaattgcc atcgttacgc tgatgttaac gcaattgatg
    11761 aacctggcgt ttattggtcc gttgaaacat gccgggctgt cactttctat tggtctggcg
    11821 gcgtgtctga atgcttcgct gctttactgg cagttgcgta agcagaaaat ctttactccg
    11881 caacccggct ggatggcgtt tctgttgcgt ctggtggtgg cggtactggt gatgtcttgc
    11941 gtgcttttag gtatgttaca tatcatgccg gagtggtcat tgggtaccat gccctggcgc
    12001 ttactgcgtt taatggcggt cgtgctggcg gggattgccg cgtacttcgc tgcactggcg
    12061 gtactgggct tcaaagttaa agaatttgcc cgccggacgg tgtaacaatg cattccggcc
    12121 tgcagtgcag gccggagata atcttcagat cgaaatcttt ttaccgccgc gatgggttgt
    12181 tgaggtttga ccgttcgccc catatagcgt cggttcctga tgcggtttca acatttccag
    12241 cgcctgttga ttgcgctcaa tctgtccttc cagtaaccaa ccgttatgtt gattcatttg
    12301 gcgtagttgc tgcgttttca cagtaatttc ctgccagcgt tgactaatat caacgctatt
    12361 tgctgtattg ggttctttct tgcgtaactg ttcgaggtaa tccagcgtcg ccagcagtga
    12421 gcttttttgt tctgtaatcc attgcaactg gctgccgttg atctgcccca tagagagatg
    12481 ttgctgctct tgatccatta ccgttttgag atcgttaagc acagcggaca tctggtcaag
    12541 gatctctgca agacgtgtca tacgatcagt tactctgcaa gtcttgctga gcttcgttga
    12601 tcagcgcatc ggcaattttg ccgatgtcca tttttagttc accgttacga atcgccagtt
    12661 ttaacgcttc gacacgttca agattgatat cactgctgcc gggttgcatc agttttgctt
    12721 gcgcgtcgct taacgtcaca ctggtgctgg tgctggtgga gtcggttgtt tttgccgccc
    12781 gggtgttcgt taccggcgcg tcagtggttt cgcgcggttg aacggtgctt acgggattca
    12841 gaggcgaagt gcgatcaata ctcatggttt attcctcatt gagggcgctt ttatcatgtg
    12901 ttgcttattt atcggcaagg gacgggtaat ctttaacagc ttacaggttt ataagaatat
    12961 tcccatctgc atcaacaacg ccgctgacca ccagtcccga taccatgcgc acccgcgcat
    13021 tctgtgcgac ggctgcattg ttcagcgcct gaccttctgc gttggcgcta aacccgtcac
    13081 cgctggcgat cacgttgacg cgttgtcccg cttttacccg ccatgcctgg cgaaaatggg
    13141 ttaattggat tggttgatcg ggtgatagat cgcgtaggct aatggcatca acaagttgat
    13201 tgatatccag caccgtacgc ggtggcaggg tatccagtcg tccgcgtttt agtttgacat
    13261 tgccagcttc cagctttcct ccccgcacaa tgggcatcgc ggcaaccaca taatttcctg
    13321 tggcctgcac attaacctgt aaatatcgtt tgtcgttacc gcagcgtgcc aacacattca
    13381 cattgcccca caggcgggaa ttattgctca tcgaaagcaa tggctgctcg cacggcggta
    13441 gtagattggg tgctgtacga atagaaacac gaacctcatc acttaccccc acgagctggg
    13501 cgctaaaaaa ggtgtgcaat tgcgatgtga gattgctcgc cgcacttaac ggactgaaca
    13561 gtatcgcgat gatcgccacg ctacgtttta ttgccagcat tttcgccccc cagctatttc
    13621 tacaacgtga attgtacccg tccccaatgg tcatcaacgg cataaatagc gacccatttt
    13681 gcgtttattc cgccgataac gcgcgcgtaa aggcatttaa gctgatggca gaattttgat
    13741 acctgcggag gagatatgct cgataagctc gacgccgcct tacgttttca acaagaggcg
    13801 ctcaatctgc gcgcccagcg tcaggaagtg ctggcagcaa acatcgccaa tgccgatacc
    13861 cctggttatc aggcgcgcga tatcgatttt gccagtgaac ttaaaaaagt catgcaacgt
    13921 ggacgggatg caaccagtgt ggttgctctg acgatgacct caacgcaaca cattccggcg
    13981 caggcgctga cgcctcctac cgcagaactg caataccgca ttccggacca gccttcgctt
    14041 gacggtaata ccgtcgatat ggatcgcgaa cgcacccagt ttgccgataa cagcctgcaa
    14101 taccagatga gccttagtgc gttgagcggg caaatcaaag gcatgatgaa cgttttacag
    14161 agcggaaatt aacggatggc actgctgaat atttttgata tcgccgggtc ggcgttaact
    14221 gcccagttcc agcgcctgaa cgtggcggcc agtaatctgg cgaatgctga tagcgtgacc
    14281 ggtcccgatg gacagccata tcgggcaaaa caggtggtat tccaggttaa cgctgcacca
    14341 ggtgctgcga caggcggcgt aaaggttgcc gatgttatag aaagtcaggc cccggacaaa
    14401 ctggtttatg aaccgggtaa tccgctggcg gatgcaaagg gctacgtaaa aatgccgaac
    14461 gttgatgttg tcggagagat ggttaacacc atgtcggcgt cacgcagcta tcaggccaat
    14521 gttgaagtgc tcaacacggt gaaaagcatg atgctgaaaa cccttacgct cggtcaataa
    14581 aggagaaagc tatgtccatt gcggtaacca ccaccgatcc gacaaatacc ggcgtcagta
    14641 ccaccagcag tagttcgctc acgggcagca acgccgcaga tttacaaagc agttttctga
    14701 ctttgctggt ggcgcagctg aaaaactagg acccgaccaa tccaatggaa aacaacgagc
    14761 tgacgtcgca attggcacaa atcagcacgg tcagcgggat tgaaaaactc aataccacgc
    14821 tcggatctat ttccggacag attgataaca gccagtcgtt acaggccagt aacctgatcg
    14881 gtcacggcgt gatgatcccc ggcacaattg ttcttgcggg aaccggcagt gaagaagggg
    14941 ctgtgaccac gaccacgccg tttggtgttg agctgcaaca ggcggcagac aaagttacgg
    15001 ccaccatcac cgataaaaat ggcgcggttg tgcgcaccat tgatattggt gaactgaccg
    15061 ccggagttca cagtttcacc tgggacggta cgttgactga tggcagcact gcgccgaacg
    15121 gttcttacaa tgtagcgatt accgccagta acggtggtac acaactggtt gcccagccgc
    15181 tgcagtttgc tctggtgcag ggtgtgatcc gcggcaacag cggtaatacg ctggatctcg
    15241 gcacttacgg caccaccacc ctcgacgaag tacggcagat aatttaagcc ttcacatttc
    15301 aggagtcagt catggccttt tctcaagcgg ttagcggatt aaacgctgcc gccactaacc
    15361 tcgatgttat tggcaacaat atcgccaact ccgccaccta cggctttaaa tcaggcacgg
    15421 cctcttttgc cgatatgttt gccggttcga aagtgggact gggggtaaaa gttgccggta
    15481 tcactcagga ctttacagat ggcacgacca ccaacaccgg gcgaggtctg gacgttgcta
    15541 tcagccagaa cggttttttc cgtctggtag acagcaacgg ttcggtgttc tacagccgta
    15601 acggacaatt taagctggat gaaaaccgta acctggtgaa tatgcaaggt ttacagctga
    15661 cgggttaccc ggcaaccggt acgccgccga ctattcagca aggggcgaat ccgaccaata
    15721 tttcgatccc gaataccctg atggcagcga aaactaccac cacggcgtcg atgcagatca
    15781 acctgaattc cagtgatccg cttcctactg ttacgccatt cagcgccagc aatgcggata
    15841 gctataacaa aaaaggttcg gtgactgttt tcgacagtca gggtaatgct catgacatga
    15901 gcgtctactt tgtgaagacc ggggataata actggcaggt ctacacccag gatagcagtg
    15961 atccaaacag cattgcgaag acagcgacaa cactggaatt taatgctaat ggcacattag
    16021 tgaatggtgc gatggcgaat aatatcgcaa ccggcgcaat taacggtgca gaacccgcca
    16081 cgtttagtct gagcttcctc aactccatgc agcaaaatac cggcgctaac aatattgtgg
    16141 caacccccca gaacggctac aaaccgggcg atctggtgag ttatcaaatc aatgatgacg
    16201 gtacggttgt cggcaactat tccaacgaac aaactcaact gctggggcag attgtactgg
    16261 cgaactttgc caacaacgaa ggtctggcat ccgaaggcga caacgtctgg tctgcgacgc
    16321 aatcttctgg cgtggcgctg ttggggacag ccgggacggg aaactttgcc accctgacca
    16381 acggtgcgct ggaagcgtcc aacgtcgatc tcagtaaaga actggtcaat atgatcgttg
    16441 cccagcgtaa ctatcagtct aacgcccaga ccatcaaaac ccaggaccag atcctcaaaa
    16501 cgctggttaa cttacgctaa tcgctgacgg gatagctcaa tggatcacgc aatttatact
    16561 gcgatgggag cagccagcca gacgcggaat caacaggcgg taaccgccag taatctggcc
    16621 aatgcctcta cgcccggttt tcgcgcgcag ttaaacgcct tgcgcgcagt gccggtggaa
    16681 gggctttcac tgcccacgcg cacgctggta acggcgtcaa caccgggtgc agacatgacg
    16741 cccggcaaga tagattacac ctcgcgcccg ctggacgttg cgttgcagca ggatggctgg
    16801 ctggccgtgc agaccgctga cggcagcgaa gggtatacgc gtaatggcag cattcaggtt
    16861 gatcccaccg ggcaactgac aattcagggg catcaggtga taggcgaggc ggggccaatt
    16921 gctgtgccgg aaggagcgga aatcactatt gctgccgatg gcacaatctc ggcgctcaat
    16981 ccgggcgatc cggcaaatac agttgcgcca gtagggtgtc tgaaactggt gaaagccacg
    17041 ggcagcgaag tgcagcgcgg tgacgacggc atttttcgtt taagcgcaga aacccaggcc
    17101 acgcgagggc cggtactgca ggcagatcca accttgcgtg tgatgtcggg ggttctggaa
    17161 ggcagtaacg tcaatgccgt tgcggcaatg agcgacatga ttgccagcgc gcggcgtttt
    17221 gaaatgcaga tgaaggtgat cagcagcgtc gatgataacg caggccgtgc caaccaactg
    17281 ctgtcgatga gttaattgaa cgattgatga cattgcaata aaagcgcgcg gactattccc
    17341 tcgccccttt ggggagaggg tgagggcgag gggaaattca cactgtcgcg tgcttctgct
    17401 ctgcttgtca ctaatccact acaggacatt ttatgatcag ttcattatgg atcgccaaaa
    17461 cgggccttga cgcccagcaa accaatatgg acgtcattgc caacaacctg gcaaacgtca
    17521 gtactaacgg ttttaagcgt cagcgcgcgg tgtttgaaga tctgctttat caaaccattc
    17581 gccagccggg ggcacagtct tccgaacaaa ccaccttacc ctccggatta caaatcggca
    17641 cgggggtacg cccggtcgcc actgaacgct tacacagcca gggaaacctg tcgcagacca
    17701 acaacagcaa agatgtcgcg attaaagggc agggcttttt ccaggtgatg ttgccagatg
    17761 gttcatcagc ctatacccgt gacggctctt tccaggtgga tcagaacggg cagctggtga
    17821 cggctggtgg ttttcaggtg cagccagcga tcaccattcc ggcgaatgcg ttaagtatca
    17881 ccatcggtcg tgatggcgtg gtcagcgtaa cccaacaagg ccaggcagct ccggttcagg
    17941 ttgggcagct caatctcacc acctttatga atgacaccgg gctggagagc attggtgaaa
    18001 acctctacac cgaaacgcaa tcctctggtg caccgaacga aagcacgccg ggcctgaacg
    18061 gcgcgggatt gctgtatcaa gggtatgttg aaacgtctaa cgtcaacgtg gcggaagaac
    18121 tggtcaatat gattcaggtg caacgcgctt acgaaatcaa cagtaaagcg gtgtccacca
    18181 ccgatcagat gctgcaaaaa ctgacgcaac tctaaggctt aaccggtggc aggttcaccg
    18241 gtttactgat ttttgaagat gatagccatg caaaaaaacg ctgcgcatac ttatgccatt
    18301 tccagcttgt tggtgctttc actaaccggc tgcgcctgga taccctccac gccgctggtg
    18361 cagggggcga ccagtgcaca accggttccc ggtccgacgc ccgtcgccaa cggttctatt
    18421 ttccagtctg ctcagccgat taactatggc tatcaaccgc tgtttgaaga tcgtcgacca
    18481 cgcaatattg gcgatacgct gaccatcgtg ttgcaggaga acgtcagcgc cagcaaaagc
    18541 tcctctgcga atgccagccg tgacggtaaa actaattttg gctttgatac tgtgccgcgc
    18601 tatttgcagg ggctgtttgg taacgttcgt gccgatgtcg aagcctccgg tggtaacacg
    18661 ttcaacggaa agggcggggc caatgccagc aataccttta gcggcacgtt gacggtgacg
    18721 gttgaccagg tactggtcaa cggcaacctg catgtggtgg gtgaaaaaca gattgccatt
    18781 aatcagggta ccgaatttat tcgcttctct ggcgtggtta atccacgcac tatcagcggc
    18841 agcaataccg taccgtctac tcaggtggtg gatgcgcgca ttgaatacgt aggcaatggc
    18901 tacattaacg aagcgcaaaa tatgggctgg ttgcagcgtt tcttccttaa cctgtcgcca
    18961 atgtaagtga ggctgtggtg attaaatttc tctctgcatt aattcttcta ctggtcacga
    19021 cggcggctca ggctgagcgt attcgcgatc tcaccagtgt tcagggggta aggcaaaact
    19081 cactgattgg ctatggtctg gtggtggggc tggatggcac cggtgaccag acaacccaga
    19141 cgccgtttac cacacaaacg cttaataaca tgctctcaca gctgggaatt accgttccga
    19201 cgggcaccaa tatgcagcta aaaaacgtcg ctgcggtaat ggtgacagcg tcacttcctc
    19261 cgtttggacg tcaggggcaa accatcgatg tggtggtttc ttccatgggt aatgccaaaa
    19321 gcttgcgtgg aggtacgttg ttgatgacac cgcttaaggg cgttgacagt caggtgtatg
    19381 cgctggcgca gggcaatatt ctggttggcg gcgcaggagc ctccgctggc ggtagcagtg
    19441 ttcaggttaa ccaactgaac ggtggacgga tcaccaatgg tgcggttatt gaacgtgaat
    19501 tgcccagcca gtttggcgtc gggaataccc ttaatttgca acttaacgac gaagatttca
    19561 gcatggcgca gcaaatcgct gacaccatca accgcgtgcg tggatatggc agcgccaccg
    19621 cgttagatgc gcggactatt caggtgcgcg taccgagtgg caacagttcc caggtccgct
    19681 tccttgccga tatccagaat atgcaggtta atgtcacccc gcaggacgct aaagtagtga
    19741 ttaactcgcg caccggttcg gtggtgatga atcgcgaagt gaccctcgac agctgcgcgg
    19801 tagcgcaggg gaatctctca gtaacagtta atcgtcaggc caatgtcagc cagccagata
    19861 caccgtttgg tggtggacag actgtggtta ctccacaaac gcagatcgat ttacgccaga
    19921 gcggcggttc gctgcaaagc gtacgttcca gcgccaggct caataacgtg gtgcgcgcgc
    19981 tcaatgcgct gggcgctacg ccgatggatc tgatgtccat actgcaatca atgcaaagtg
    20041 cgggatgtct gcgggcaaaa ctggaaatca tctgatgatc agcgacagca aactactggc
    20101 aagtgcggcc tgggatgcgc aatcactcaa cgaactaaag gcgaaagcgg gcgaagatcc
    20161 ggcggcaaat atccgtccgg tggcccgtca ggtggaaggg atgttcgtgc agatgatgtt
    20221 gaaaagcatg cgcgacgctt taccaaaaga tggcctgttc agcagcgagc acacttgcct
    20281 gtataccagt atgtatgacc agcagattgc ccaacagatg acggcgggca aaggtctggg
    20341 gcttgcagag atgatggtta aacagatgac gccagaacaa ccattgccag aggagtccac
    20401 gccagcagca ccgatgaaat tcccgctcga aactgtggtg cgttatcaaa atcaggcgtt
    20461 ttcgcagctg gtgcaaaagg ccgtgccacg taactacgat gattcgctgc cgggtgacag
    20521 taaagcattc ctcgcgcaac tctcgctgcc cgcccaactg gcaagccagc aaagcggtgt
    20581 gccacatcat ttgatcctcg ctcaggcggc gctggaatct ggttgggggc aacggcaaat
    20641 ccgccgcgaa aacggcgagc cgagctataa cctgtttggt gtcaaagcct ctggcaactg
    20701 gaaagggcca gttactgaaa tcaccacgac tgaatatgaa aacggcgaag cgaagaaagt
    20761 aaaagcgaag tttcgggtct acagctcgta tctggaagca tagtcggatt acgttgggct
    20821 gttaacgcgt aacccgcgct acgccgccgt gaccaccgcc acgagtgcgg agcagggggc
    20881 gcaggcccta caggacgcgg gctatgccac cgatcctcac tatgcccgca aactcaccaa
    20941 catgattcag cagatgaaat cgataagcga caaggtgagc aaaacctaca gtatgaacat
    21001 tgataatctg ttctgaataa ctcaagtccg gcgggtcgct gccgataata ctctgtaatt
    21061 gaaggcttat aaggaacctc catgtccagc ttgattaata acgccatgag cggactgaac
    21121 gcggcccagg cggcgttaaa tacggcaagt aataatatct ccagctataa cgttgccgga
    21181 tatacccgcc aaaccactat tatggcgcag gccaatagca cgttgggcgc tggcggctgg
    21241 gttggcaatg gtgtctacgt ttctggtgtg cagcgtgagt atgatgcgtt tatcaccaac
    21301 cagttacgtg cggcgcagac gcaaagtagc ggcctgactg cccgctatga gcagatgtcg
    21361 aaaatcgaca atatgctctc caccagtacc tcttcgctgg caacacagat gcaggatttc
    21421 ttcaccagcc tgcaaacgct ggtgagtaac gcggaagacc cggcagcgcg ccaggcgctg
    21481 attgggaaat cagaagggtt ggtgaatcag tttaaaacca ccgatcagta tcttcgcgac
    21541 caggacaaac aggtcaatat cgcgataggt gccagcgttg atcagatcaa caattacgct
    21601 aaacaaatcg ccagcctgaa cgaccaaatc tcccgcctga caggcgtggg ggcaggggcg
    21661 tcacctaaca atctgctgga tcaacgcgat caactggtga gcgaattaaa ccagattgtt
    21721 ggtgtagaag tcagcgttca ggatggcggc acttataaca tcacgatggc caatggttac
    21781 tcactggttc agggaagtac ggcgcggcaa ctggcggcag ttccttccag cgccgaccct
    21841 tctcgtacga ctgtcgctta cgttgatggg acggcaggca atattgagat cccggagaaa
    21901 ttactgaata ccgggtcgct gggcggcatt ctgacattcc gttctcagga tctggaccag
    21961 acgcgtaata cgcttggaca actggcgctg gcatttgccg aggctttcaa cacccaacac
    22021 aaagccggat ttgatgccaa cggcgatgct ggtgaagatt tctttgctat cggtaagccc
    22081 gcggttttgc aaaacacgaa aaacaaaggt gacgttgcga tcggtgccac ggtaactgat
    22141 gcctccgcgg tactggcgac agattacaaa atctcgttcg ataataatca gtggcaggtc
    22201 acccgccttg ccagcaatac cacttttacg gtgacgccgg atgccaacgg taaagtggca
    22261 tttgatggtc tggagttgac gtttacagga acgcctgccg ttaacgacag cttcacgctg
    22321 aaaccagtaa gtgacgccat cgtcaacatg gatgtattaa tcaccgacga agcgaaaata
    22381 gcgatggcga gcgaaaaaga tgcgggtgat agcgataacc gcaacggtca ggccctgctg
    22441 gatctgcaaa gcaacagtaa aacggtgggc ggtgcgaaat cctttaacga cgcttatgcc
    22501 tcgttagtga gtgatatcgg taataaaacc gcgacgttga aaaccagtag cgccacgcaa
    22561 ggtaatgtgg tgacgcagct ttccaatcag cagcagtcta tctctggcgt caatctcgac
    22621 gaagagtacg gaaatctgca acgttttcag cagtattacc tggcgaatgc gcaggttctg
    22681 cagacggcaa acgcgatttt tgatgcgctg attaacattc gctaagggga gataagatgc
    22741 gtttcagtac ccagatgatg taccagcaaa acatgcgtgg catcaccaat tctcaggcgg
    22801 aatggatgaa gtacggcgag cagatgtcga caggtaagcg agtcgttaac ccttctgacg
    22861 atcccattgc agcatcacaa gccgtagttc tctcccaggc acaggcgcaa aacagccagt
    22921 acacgctggc gcgtactttc gccactcaaa aagtgtcact ggaagagagt gtacttagcc
    22981 aggtcaccac tgctatccag aatgctcagg aaaaaattgt ctacgccagc aatggcacct
    23041 tgagtgacga tgaccgggcc tcgctggcta cggatattca ggggcttcgt gaccagttgc
    23101 tgaatctggc gaacaccact gacggtaacg ggcgctacat ttttgccggt tataaaacag
    23161 agactgcgcc gtttagcgaa gagaaaggga aatacgtcgg tggagcagaa agtattaagc
    23221 aacaggtcga tgcttcgcgt tcgatggtga tagggcacac gggtgacaaa attttcgaca
    23281 gtattaccag caacgcagtt gcggagccag acggtagcgc ttctgaaacc aatctttttg
    23341 ccatgctgga tagcgccatc gcagccctga aaacgccggt cgcggatagc gaagcggata
    23401 aagaaatcgc cgctgcggca ttggataaaa ccaaccgtgg actgaaaaac tcgctgaata
    23461 atgtccttac cgttcgcgcg gaattaggca cgcagctgaa cgaactggag tcgctggatt
    23521 ccttaggtag cgatcgcgct ttagggcaaa cgcagcagat gagcgatctg gttgatgtgg
    23581 actggaatgc aactatttca tcttacatca tgcagcaaac ggcattgcag gcatcgtata
    23641 aagcatttac cgatatgcag ggattgtcgc tcttccagct caacaaataa gctcgcttta
    23701 aaacatatca tgaaactggg tatgttttgt ctgcccgctc agggatcgcc ggggcgggca
    23761 tttttttgcc tatttagcat tgttggttag caaggatgcc atttgatgaa ttctaatatg
    23821 ttgattcaaa catgaaataa aaaagccctg gcagttacca gggcttgatt actttgagct
    23881 aattattact caacaggttg cggacgcgca ggagcggcag aggcatgatg cgttgccgta
    23941 tgaccacctg cggcaccttt accttcgaag gcaaaagtag ggcgctgcca gtcactgtga
    24001 tgcggtgcct ccggaacata ttccggtgcc ggagcgcgcg tcattggcgc ggtagcgtgg
    24061 ttatgctcaa cggtgatctc tggttcaacc gcggctaccg tttcaacttc tgctgcgact
    24121 tcagcgacga ctggcgcggc aggttgagca acaacttcag gttctgcaac cacaacctca
    24181 gcagtttcgg caacttcttc aatatctgcc gtctcttcct gcggttcaac caccggttct
    24241 gcgtgttctg caacttcctg ggctacggca acatcagact cggtaatcac ctgcggctgt
    24301 tcagttaccg cggcggcaat cacttcagga tgcgtcgttt caaccacttc cggttgcggt
    24361 tcggcaaccg gtacgggggc ttctacgact tcagccattt cttcaacaac tggcgcgcta
    24421 acaaccggtt cgacagcggc ggcgacaggg acctcagtca ccatcggctg cacttgtact
    24481 tcttcctgtt cgcgctgctc ttcaacttgt acatcttgcg gacgtacaat tggatagcgg
    24541 atccagactt tgccagaggc cagttccgga gacgcgcacg ctacggtcaa cggcattggc
    24601 gactgggttg gataacgctc gtcacgatag cgacgacgac gctgaccact cacgcgcagg
    24661 tgacgaggcg agcggcgaga acgacgcggc atgccaccgt tgtcacggtt atcagcattg
    24721 ttctcttctt gctgttctgg tgcagtttgc gctacgactg gcagcgggac tttcaccagt
    24781 tctgtgcgtg gagctggagc ttcctgaaca attggttcgg cagcggcagt ttcttcaacc
    24841 accggtgcga ctaccgcttc ttcggctacg ctttgctcgt aacgcacttt ctgattgagc
    24901 tgacgctgtt tacgacgcgg ctgaaccgga cgtacacgtt cttcctgttc ggtttcctga
    24961 acagattgct cttcaacatt cagcgccttc gcttcttgtt gcgcctgacg tttatcatcg
    25021 ttacggcggc ggctacgttc acgacgcggc gcttgctgct cgtcggtggt acgtgctttt
    25081 tccgttacct cagcttgctg acggctctca cgcgtctcgg cagtctgctg ctgtgcctgg
    25141 cgacgattac gacggttttc ttcgcgatta tcgctgcctt cagtacgttc actacgggtg
    25201 tcgcggcgct cataacggtc acggcggttg ttctgacgag gcttgcgacg atcctgttga
    25261 cgttccggtt tcgcttctgc tttcggtgct ggttgctcgg tcggtttggt ttcttcacta
    25321 ccgctgaaca gcgctttcag tgcgccgaag aagcggctca acagcccagg ttgtgcagga
    25381 gctgctggtg ttgccggtgc agctttcggt gctggagcta caacaggcgc ggcaggttca
    25441 gctggcgttg gcgctggcgg cacatccggc atggcaaagg ttgccagcgc aggttgttcc
    25501 ggacgcttac gttcagcgaa ctcttcttca gacggcagcg ccatcgcttc ttcatgcagc
    25561 ttcggcagca tgtagcttaa ggttggggtt tcttcccctt tacgcacgcg cagcacgtgg
    25621 tagtgcgggg tttccatctg atcgtttggc acaatcacgc agcgcacacc gtcctgacga
    25681 gtttcaatgg catttaccgc agaacgtttt tcattcagca ggtaagaagc gattggtaca
    25741 ggaacaatgg cgtgaacttc ctgggtgttc tctttcagcg cttcttcttc gatcagacgc
    25801 agaatagaga gcgacagcga ttcgttgtca cgcacggtgc cagtaccaga acaacgcgga
    25861 caaacgtgat gactggattc acccagtgat gggctcaggc gctgacggga catttccagc
    25921 aggccaaagc gagaaatatg gctgatttga atacgcgcac ggtcctgacg caccgcttca
    25981 cgcagacggt tttctaccgc acgctggtgg cgtacaggcg tcatgtcgat gaagtcgata
    26041 acaatcaggc cgccgaggtc acgcaggcgc agctgacgag caatctcatc ggcagcttcg
    26101 aggttggtgt taaacgcggt ttcttcgata tcgccgccgc gggtcgcgcg tgcggagttg
    26161 atgtcgatgg cagttaacgc ttcggtactg tcgataacaa tcgaaccacc ggacggcaga
    26221 cggacttcac gctggaaggc ggactcgatc tgcgactcga tctggtagtg gctgaacagc
    26281 gggatctcgc cggtgtacag tttgattttg ctgctgaaat ccgggcgacc taatgcagcg
    26341 atatgctgac gtgccagttc gagcactttc gggttatcga taaggatttc gccgatgtcc
    26401 tggcgtaagt aatcgcggaa agcgcgaacg attacgttgc tctcctgatg aatcaggaac
    26461 ggggccgggc ggctttcagc ggcttttttg atggcttccc agtgtttcag acggaagctt
    26521 aaatcccatt gcagcgcctc agcagatttg ccgacgccag cggtgcgcac gataagcccc
    26581 atgccttccg gcagttcaag gcttgccagt gcttctttta attcggtacg gtcgtcgcct
    26641 tcgatacggc gagaaatgcc acccgcgcgc gggttgttcg gcatcagaac cagatagcta
    26701 cccgccagac tgataaaggt ggttaatgcc gcgcctttgt tgccgcgctc ttctttatcg
    26761 atctgaacaa tgacttcctg accttcacgc aacacatctt taatgttggg acgaccatga
    26821 gcactgtagt tagcagggaa atattcgcgg gcaatttctt ttagtgggag gaaaccgtga
    26881 cgttcagcgc cgtaatcaac aaaagcagct tccagactcg gttcaatgcg ggtgatttta
    26941 cctttgtaga tgtttgcctt tttctgctcg tgccctggac tttcgatatc caggtcatac
    27001 agacgctgcc catctacaag ggcaacgcgc aactcttcct gctgagttgc gttgattaac
    27061 attcttttca tcgtaactta ctcattattc ttacattgac gactaagctg cgggcaaagt
    27121 aacgcctttc cgggtgtgaa ccgatggcct cgtgtctagt cgcgtcgcca acctcacggt
    27181 tatcgtcagc tcaaagaggc gcagagtgtc ggttgcccgt ttttcatgcg gaaaaacagc
    27241 gcaattatca aagaaacaga ctgggtatta ctctccagaa atatttccat ctgccggtaa
    27301 ggactgcaac ccgcagcccg ctaactgcct gaaagatcaa tacgtcttac gccattgctg
    27361 cgtggatgat cggtcgggca aaatgggtta ttccgtaaaa tttcttgttt taacaaggat
    27421 ggacacggaa acggcttcat tattccactg caagccttgt tatagcaaga tgacttttac
    27481 catttatcac ccgcttactc acagtt
//
LOCUS       DAJTMK010000038        27258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTMK010000038 DAJTMK010000000
VERSION     DAJTMK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27258
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.039"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..324
                     /locus_tag="OO135_001392"
     CDS             <1..324
                     /locus_tag="OO135_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR5397301.1"
                     /translation="FAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDLFLFSDKIFS
                     IAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGGG
                     ASGRW"
     gene            complement(384..1682)
                     /gene="eno"
                     /locus_tag="OO135_001393"
     CDS             complement(384..1682)
                     /gene="eno"
                     /locus_tag="OO135_001393"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5397302.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1770..3407)
                     /gene="pyrG"
                     /locus_tag="OO135_001394"
     CDS             complement(1770..3407)
                     /gene="pyrG"
                     /locus_tag="OO135_001394"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5397303.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3630..4421)
                     /gene="mazG"
                     /locus_tag="OO135_001395"
     CDS             complement(3630..4421)
                     /gene="mazG"
                     /locus_tag="OO135_001395"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5397304.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4492..4827)
                     /gene="mazF"
                     /locus_tag="OO135_001396"
     CDS             complement(4492..4827)
                     /gene="mazF"
                     /locus_tag="OO135_001396"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR5397305.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4827..5075)
                     /gene="mazE"
                     /locus_tag="OO135_001397"
     CDS             complement(4827..5075)
                     /gene="mazE"
                     /locus_tag="OO135_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR5397306.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5153..7387)
                     /locus_tag="OO135_001398"
     CDS             complement(5153..7387)
                     /locus_tag="OO135_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5397307.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7435..8736)
                     /gene="rlmD"
                     /locus_tag="OO135_001399"
     CDS             complement(7435..8736)
                     /gene="rlmD"
                     /locus_tag="OO135_001399"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5397308.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8793..11549
                     /gene="barA"
                     /locus_tag="OO135_001400"
     CDS             8793..11549
                     /gene="barA"
                     /locus_tag="OO135_001400"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5397309.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11781..13121)
                     /gene="gudD"
                     /locus_tag="OO135_001401"
     CDS             complement(11781..13121)
                     /gene="gudD"
                     /locus_tag="OO135_001401"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5397310.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13142..14482)
                     /locus_tag="OO135_001402"
     CDS             complement(13142..14482)
                     /locus_tag="OO135_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5397311.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTQVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAVPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14484..15854)
                     /gene="gudP"
                     /locus_tag="OO135_001403"
     CDS             complement(14484..15854)
                     /gene="gudP"
                     /locus_tag="OO135_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5397312.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGSLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(16271..16720)
                     /locus_tag="OO135_001404"
     CDS             complement(16271..16720)
                     /locus_tag="OO135_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5397313.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16738..17520)
                     /gene="truC"
                     /locus_tag="OO135_001405"
     CDS             complement(16738..17520)
                     /gene="truC"
                     /locus_tag="OO135_001405"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5397314.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17520..17849)
                     /locus_tag="OO135_001406"
     CDS             complement(17520..17849)
                     /locus_tag="OO135_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5397315.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18471..19016)
                     /gene="syd"
                     /locus_tag="OO135_001407"
     CDS             complement(18471..19016)
                     /gene="syd"
                     /locus_tag="OO135_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5397316.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGYIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19084..19932
                     /gene="queF"
                     /locus_tag="OO135_001408"
     CDS             19084..19932
                     /gene="queF"
                     /locus_tag="OO135_001408"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5397317.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20044..21408
                     /gene="ppnN"
                     /locus_tag="OO135_001409"
                     /gene_synonym="ygdH"
     CDS             20044..21408
                     /gene="ppnN"
                     /locus_tag="OO135_001409"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5397318.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21965..23254
                     /gene="sdaC"
                     /locus_tag="OO135_001410"
     CDS             21965..23254
                     /gene="sdaC"
                     /locus_tag="OO135_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5397319.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRVILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWIAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23312..24679
                     /gene="sdaB"
                     /locus_tag="OO135_001411"
     CDS             23312..24679
                     /gene="sdaB"
                     /locus_tag="OO135_001411"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5397320.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24791..25546
                     /gene="xni"
                     /locus_tag="OO135_001412"
     CDS             24791..25546
                     /gene="xni"
                     /locus_tag="OO135_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5397321.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKSGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25651..26788)
                     /gene="fucO"
                     /locus_tag="OO135_001413"
                     /pseudo
     CDS             complement(25651..26788)
                     /gene="fucO"
                     /locus_tag="OO135_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26816..>27258)
                     /locus_tag="OO135_001414"
     CDS             complement(26816..>27258)
                     /locus_tag="OO135_001414"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5397322.1"
                     /translation="PSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRPIPAIHY
                     MIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIACEVNLEKALWLA
                     HEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6581 a         7291 c         6576 g         6810 t
ORIGIN      
        1 tttgcagtga tgttcttccc tttctggttt tttcatcaag gcagtaattt ttgtcgcgca
       61 tgtaaaagtg gagtctgtat ttcagcaatc tatcttttag atttattcct gttctccgat
      121 aaaatttttt ccattgctgt attttccttc ttttttactt tcaccatatt tatggtcttt
      181 acctgtttat gtgtacttca gaaaagagca tcaggtaaaa gttatcattc agacaacagc
      241 ggttccggtg gaggctcaag tggcggcgga tttagcggcg gtggcggttc ttctggaggt
      301 ggcggcgcat ccggccgctg gtaactcaac cataaaaaat gccatcccgg aggctggcat
      361 ttttaaatca gataaagtca gtcttatgcc tggcctttga tctctttacg accgttgtac
      421 ggtgcttttt cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg
      481 tcagaacggc tcatagaacc agttttgatc tggcctgcag cagtaccaac agccaggtca
      541 gcgatggtag cgtcttcagt ttcgccagaa cggtgagaga taactgcagt gtagccagca
      601 tctttcgcca tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg
      661 atcaggatgg agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt
      721 acgaacaggt cgtcaccaac cagctggatt ttgtcgccca gaactttagt ctggtatgcg
      781 aaaccgtccc agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg
      841 gtcagttctt ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca
      901 gccagaacgt atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagta
      961 atgtctttgc ccagttcata accagcagct ttaacagctt cagcgataac agccagagct
     1021 tcagcgttgg aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg
     1081 cctttcgctt tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct
     1141 tctttcacag ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg
     1201 tcagcgtgct caccaccgtt gatgatgttc atcatcggaa ccggcataga gtatttgccc
     1261 ggagtaccgt tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca
     1321 gctttggcgt tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt
     1381 tcggtgccgt ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg
     1441 ccaatcagcg cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta
     1501 cccaggaaac gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa
     1561 gcacctgacg gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca
     1621 acagtcgggt taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac
     1681 attaggtttt cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccag
     1741 ggtacgcgtt gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg
     1801 gcggctttca caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc
     1861 ggatggaact ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac
     1921 tgatcatccc cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc
     1981 atgttgttga cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtacagc
     2041 tggcgaacca ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg
     2101 ccgagatcgc tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta
     2161 atcagcgcca caaccgggta cttacagtct ggcacaaatt ccgtagagtt ggcgttctcc
     2221 atgttggcaa catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc
     2281 agataaggaa tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca
     2341 cgatagccga aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg
     2401 cgcgtttcaa catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc
     2461 ccaccgtgtt tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg
     2521 ccgaccatac cgatggtgac ttcacttacc gggttcgctt cttcgaagat aacctgttcc
     2581 cattcggaca gattcgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc
     2641 agcccctgag atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa
     2701 ataaccgctt tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccgga
     2761 acagcgcgat ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct
     2821 tttacagagt gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc
     2881 agcgtcaggt gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaatc
     2941 gcttcgagga acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact
     3001 acgtcatgac cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga
     3061 ataacctgca cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag
     3121 tagatacgac ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg
     3181 tagtgcccca ggtccaggtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt
     3241 tggattgggc tcatagtacc tggatcgacg ttgatgtacg gatccagttt catgatggtc
     3301 acattgaggc cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc
     3361 agagaggata cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga
     3421 agttaggttg aaaagacgat ggaataacca ggacgggaaa gcagtatacc cgaacatgac
     3481 ctgtgccaca aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg
     3541 agaaaatagc ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa
     3601 aatgacaagc gcttgaccgc gtaattccct tagagatcaa tttcctgccg ttttacctgt
     3661 tgccagactt cttccattgt ttcgaggtca acacctgtca tttccagtcc acgcgcggca
     3721 acaatacgct ccacttcgcg aaaacgacgc tcgaattttt cgttcgcttt ttgcaatgcg
     3781 atttctgctt tcgtccctaa atggcgagcc agattaaccg tggcaaacag caggtccccc
     3841 atttcctcct ccagtttagc ctggtcgaca acagcctgcc gcgcctcgta catcacctcg
     3901 tcgatctctt cgtagacttt atcaaccacc gggccaagcg tcgtccagtc gaagccaacg
     3961 ttggcgcaac gtttctggat tttttgcgcc cgcattaaag ccggtaaact acgaggaata
     4021 tcgtccagcg ccgaatgctg cgctttctgc gcgcgctctt cggttttgat ttgctcccaa
     4081 cgggcaagca cttcactact gttttcggca gaactatcag caaaaacatg cggatggcga
     4141 cgctctaatt tatcgctaat agcagcgcaa atatcattaa agtcaaagcg cccttcttcc
     4201 tgagccattt gcgcgtaaaa caccacctgg aatagcagat cgcccagttc accgcgaaga
     4261 tcgtcaaaat cttcacgggc gatggcgtcc agcacttcgt aggtttcttc aagggtgtaa
     4321 ggcgcaatgg tggcaaatgt ctgctcttta tcccacgggc agccgttttc cggatcgcgc
     4381 aggcgctgca taatagtgag caaacgatcg atttgattca ttgaattgtc ctgaaaattg
     4441 cgggtctgtc aggtggaaac ctgtgaccag aatagaagtg agttagtaac actacccaat
     4501 cagtacgtta attttggctt taatgagttg taattcctct ggggcaactg ttcctttctt
     4561 cgttgctcct cttgcccgcc aggcgattct ttttacctga tcagctaacg ctacgccatc
     4621 acgttcctga ccggataaaa caacttcgaa cggatatcct tttgattgcg ttgtacaagg
     4681 aacacacaga cacatacctg ttttgttgtt gtacatgaaa ggactcagga caacagctgg
     4741 acgatgtcca gcttgctcgc taccttttgt cgggtcaaaa tcaacccaaa tcagatcgcc
     4801 catatcgggt acgtatcggc ttaccattac cagacttcct tatctttcgg ctctccccag
     4861 tcgatattct cgtggaggtt ttccggcgtg atgtcgttga ccagttcagc aagcgtaaat
     4921 acgggctctt tacgcactgg ctcaataatt aatttgccat ccaccaggtc aatcttcact
     4981 tcatcatcaa tattcagatt gagcgcctgc attaacgtag ccgggatccg caccgccggt
     5041 gaatttcccc aacgctttac gctactgtgg atcataaccc tttcctcaaa ccgctatcat
     5101 atgtagatac agtatatatc aatctacatt gtagatacga gcaaatttct gcctaactcc
     5161 cgtgcaaccg acgcgcgtcg ataacatccg gcacctggtt gagtttaccc agcacgcggc
     5221 ccagcacctg caggttgtaa atctcaatgg tcatgtcgat ggtcgccagt tgctgtttgg
     5281 tgtcgctacg gctggcaacg ccaagtacgt tcaccttctc gttggcgaga atggtcgtga
     5341 tatcacgtaa caacccacta cgatcattag ccaccacgcg gaccaccagc gaatatccgg
     5401 cggagtagct ctcaccccat accgcgtcaa caatgcgttc tggcgcatga gagcgcagtt
     5461 ccgccagttg ttcgcaatcg gcgcggtgta ctgaaatacc gcgcccctgg gtaatgaagc
     5521 cgacaatctc atctccagga atcggctggc agcagcgcgc gatgtggtgc atcaggttgc
     5581 caacaccttc gactaccacg cgaccgttat ctttactgcg gttttgcggc gtgtagcttt
     5641 tttgctgaag ttgcttcagc gcggcggcgt cctgctcttc ggcactcggc ttattaaatt
     5701 gcgattgcag gaagttcacc atctgattga gacggatatc cccgccacca atcgccgcca
     5761 gcaactcgtc gacatcatta aagttgtaac gcggcagcag atgtttttct gcttctttca
     5821 ggctgatccc cagatgttcc agctcgtcgt caaggatttg ccgcccagcc agaatgtttt
     5881 tgtcacggtc ctgtttacgg aaccaggcgt gaattttcga acgcccacgg ctggttgtga
     5941 cgtaaccgag gtttgggttt aaccagtcac ggctggggtt cggctgtttc tgggttataa
     6001 tttcaatctg gtcgcccatc tgcagctggt aggtgaacgg cacaatgcgc ccgccaattt
     6061 ttgccccgat gcagcggtgt ccgacatcac tgtggatgtg gtaagcgaag tccagcggcg
     6121 ttgatcccgc aggcaaatca acgacatcac ctttcggcgt aaagacatac acccggtcgt
     6181 caaagacctg gctgcgtact tcgtcgagca tttcgccgga atcagccatc tcttcctgcc
     6241 acgcaatcag tttacgcagc caggcaatcc ggtcttcatg tcccgaacgt gcgccgccag
     6301 ctgccgcgcc ctctttatat ttccagtgcg cagcaacacc caactctgca tcttcatgca
     6361 tctgtttggt gcggatttgg atctcaacgg ttttgccacc tggccccaga accacggtat
     6421 gaatagactg ataaccgttt ggtttcgggt tagcgacata atcgtcaaac tcatccggca
     6481 ggtggcgata gtgagtgtgc actatcccca gtgcggcata acaatcctgt aaacgctcgg
     6541 cgacaatacg taccgcacgc acatcaaaca gctcatcgaa ggcgaggttc tttttctgca
     6601 ttttgcgcca gatgctgtag atgtgtttcg gacggccata cacttcagct ttaacgcctt
     6661 cagctttcat ctcagcgcgc agatgaccga caaactcttc gatatagtgt tcgcggtcga
     6721 gacggcgttc atgcagcagt tttgcgatgc gtttgtattc ggtcgggtgc agataacgga
     6781 agcagtaatc ttccagctcc catttcagtt gcccaatccc aagacggttc gccaacggcg
     6841 cgtagatatt ggtgcactct tttgccgcca gtacgcgttc atcttccggc gcatctttta
     6901 cttcgcgcag atgagcaata cgctccgcca gtttgatgac cacgcagcga aaatcatcga
     6961 ccatcgccaa taacatccgg cgaacgttat cgacctgttc ggaggaaaca gaatcagtgt
     7021 gcgtcgcttt cagctggcgg atcgccgcca tatcacgcac gccgtgaata aggttaacga
     7081 cagacttacc gacgctctca cgcagcacat cttcgctgac tacgttggca tcggccagag
     7141 ggaacagcag cgccgcccgc agcgtgtcaa tgtccatact taatgtcgag aggatctcca
     7201 ccatctcaac accacgccac aataacagac tggcatccgg atgcccctgc gtctgctgca
     7261 gacaatacgc ccaggtttcg gctaagcact cacacgactt ctggctggta atacccagac
     7321 ttgcgatcca tttttccgga tcaaattcac cagccttatt gatatgtgca cttcttaccg
     7381 caaccattgt cctctccttt agggaccaga cctgccgaaa tcggcaaatc gtaactattt
     7441 aacgcgcgag aaaagtacca tcgattccag atgtcccgtg tgtgggaaca tatccagcat
     7501 cgccagtcgc gcaatggtat atcctgcttt taataacgct tcgctatccc gagccagcgt
     7561 tgcagggtta caggatacat aaactatacg aataggttcc agttttataa tttgctgcat
     7621 aacacctgcg gcacctgctc gcgccgggtc cagcaacact ttatcgaagc cgtttttcgc
     7681 ccacggctgc tttgtgacat cttcttcaag attttcgtga taaaacgtca cattctgtaa
     7741 gccattaaga cgcgcattct gctggccttt ttccaccagc gccggaacac cttctacacc
     7801 gaccacactg gcagcttgtg tcgccaatgg cagtgtaaag ttgcccatac cgcagaacag
     7861 atccagtacg cgatcttcag gttgcacatc cagccattcc aacgcactcg ctaccatttt
     7921 ttggttcaca cccgcattga cctgaataaa atcgcgcggg ctaaaagtta agcgcaaccc
     7981 gttagagtca taccagggca tctcaccaga gacggtttcg agtatctcac tatcgggggc
     8041 gagatacaga tccaggcctt cagaatgcga aaagcgttcc agtttttcgc gatctgccga
     8101 acttagcggt gcggtatggc gcaaaatcat cagcgtgccg ctggttgcct gtaccagttc
     8161 aacatgacca agatggcgca ttgcttgtaa gctgcccagg catgccctga ctttgggcag
     8221 caatgcttca agttggggca ctaaaatggg acattgttta acgtcgacaa tgtcactgga
     8281 gcccgctttg cgaaacccca tctgaagttg ctgtgttttc ggtaagtagt tcagacttaa
     8341 acgcgcgcga cggcgatagc cccagggaac atcggcgatc acttcagaga cttcgtgttt
     8401 cattaatcgg gcgagtgccg cacttttgct tcgctgctgt aaatccacgc tggcgtgttg
     8461 ttgctgacag ccgccgcata cgccaaaatg aggacagcgt ggcgtttcgc gttccgggct
     8521 atcgcttaac cggcgtacga ctttagcgcg ggcatactgt tttttatctt cagtaacagt
     8581 aacttccgcg ttttcctgcg gcaataatcc ggggataaat agcgttttgc cgttatgtcg
     8641 cgccacgccc tgaccaaaag agtcgaggtc gttgactgaa acggttatga tctgacgcgt
     8701 cgtcgtgcgt cgttttgcag agtagaattg cgccattggc gagactttct caatttaaca
     8761 gtgtgacctt aattgtccca taacggaact ccatgaccaa ctacagcctg cgcgcacgca
     8821 tgatgattct gatcctggca ccgaccgtcc ttattggttt attgctgagt atctttttcg
     8881 tcgtgcatcg ctataacgac ttgcagcgtc aactggaaga tgccggtgcc agcattattg
     8941 agccgcttgc agtttctact gaatatggca tgagcctgca aaatcgcgaa tctatcggtc
     9001 agttaataag cgtactgcat cgtcgccatt ccgatattgt tcgcgcgatt tcggtttatg
     9061 atgaaaataa ccgactcttt gtcacctcca attttcatct tgatccctca tcaatgcagc
     9121 tcggcagcaa cgtgccgttt cctcgccagc tcactgtcac tcgtgacggc gatattatga
     9181 tcctccgcac gccgattatt tctgagagtt actcccccga cgaatcgccc agtagcgatg
     9241 ccaaaaatag tcaaaatatg ttgggatata ttgcgcttga gctggatctt aaatcggttc
     9301 gcttgcagca atataaagag atctttattt ccagcgtgat gatgctgttt tgtatcggta
     9361 ttgcgcttat ttttggctgg cgcttaatgc gcgatgtaac cggtccgatt cgcaacatgg
     9421 tgaataccgt cgaccgcatc cgtcgcgggc aactcgacag ccgggtggaa ggatttatgc
     9481 tcggcgagct ggatatgctg aaaaacggta tcaactcgat ggcaatgtcg ctggctgctt
     9541 atcacgaaga gatgcagcac aatatcgacc aggcgacgtc cgatctgcgt gaaacgctgg
     9601 agcagatgga aattcagaac gttgagttgg atctggcgaa aaagcgcgcc caggaagcgg
     9661 cgcgtattaa atccgagttt ctggcaaata tgtcacacga gctgcgtaca ccactgaatg
     9721 gtgttattgg ctttacccgc ctgacgctga aaacagaatt aacaccaacg cagcgcgatc
     9781 acctgaatac gattgaacgt tcggcaaata atttgctggc aattattaat gatgttctcg
     9841 acttctcgaa actggaagca ggtaagctga ttctggaaag tattccattc ccactacgca
     9901 gcacgctgga tgaagtcgtt actctgctgg cacattcttc tcacgataaa gggttggaac
     9961 tgacgctcaa tattaaaagc gacgtgcctg ataacgtgat cggcgaccca ctgcgattac
    10021 agcaaatcat cactaacctg gtggggaatg caattaaatt caccgagaat ggcaacattg
    10081 atattctggt agaaaaacgt gcgctgagta ataccaaagt gcagattgaa gtgcagattc
    10141 gggataccgg cattggtatt cctgaacgcg atcaatcgcg cttattccag gccttccgac
    10201 aggctgatgc cagtatttcc cgccgtcatg gtggcaccgg gctggggctg gtgattacac
    10261 aaaaactggt taatgaaatg ggcggcgata tttcgttcca tagccagccg aatcgcggtt
    10321 caactttctg gttccacatt aatctcgatc tgaacccgaa cattattatc gaagggccat
    10381 ccacccagtg cctcgcaggt aagcgcctgg cctatgtcga accaaactcc gcagcagcgc
    10441 aatgcacgct ggatatttta agtgaaacgc cgctggaagt ggtttatagc ccaacgttct
    10501 ccgcgctgcc tcccgcgcat tacgacatga tgttgttagg catcgcggtg accttccgcg
    10561 agccgctaac aatgcaacat gagcgattag cgaaagcggt atcgatgacc gatttcctga
    10621 tgctggcact tccttgccat gcacaagtca atgctgaaaa actcaagcaa gatggtatcg
    10681 gcgcgtgtct gctgaaacca ttaacaccta cacgcctgtt gcctgccctg acggaatttt
    10741 gtcatcacaa acaaaacacg cttttgcctg taaccgatga aagtaagctg gcaatgacag
    10801 tcatggcggt tgatgacaac cccgctaacc tgaaacttat cggcgcattg ctggaagata
    10861 tggtgcaaca tgtggaactt tgcgatagcg ggcatcaggc ggttgaacgg gcgaaacaga
    10921 tgccgttcga tttgatctta atggatattc aaatgccgga catggatggc attcgggcct
    10981 gcgaactcat ccaccagctc ccccatcagc aacaaacgcc ggttatcgcg gtaacggcgc
    11041 atgcaatggc cgggcaaaaa gagaagctgc ttggcgcagg gatgagcgat tatctggcga
    11101 aaccgattga agaagagcga ttgcataatt tgttgttgcg ctacaagcct ggcagcggta
    11161 tttcctctcg cgtcgtgacg cccgaagtca acgaaattgt ggtgaacccg aatgcgaccc
    11221 tcgactggca actggcacta cgccaggcag caggaaaaac cgatttagcg cgcgatatgc
    11281 tgcaaatgtt actcgatttc ctgcctgaag ttcgcaacaa agttgaggaa cagctggttg
    11341 gagaaaaccc ggaaggcctg gttgatttga ttcataaact gcatggcagt tgcggctata
    11401 gcggtgtgcc gcgtatgaag aatctctgcc aactgatcga acaacagcta cgtagcggta
    11461 ctaaagaaga agatttggag ccggagctgc tggaactgtt ggacgagatg gataatgtcg
    11521 cgcgcgaagc cagcaaaatt ctcgggtaac ggacgtcttg tcgagtagca atcggctttt
    11581 tatgacgctg gtttcagatt ggtgacaaag tgcctgtgtt tatgccggat gcggcgttaa
    11641 cgccttatcc ggcctacaaa accaattaaa ttcaatgaat taaaaaatga tgtagacctg
    11701 atacatagca caacaggcta ttttgcgttt agcatcagtc tcaagccggc tccagataga
    11761 gccggttttg gttttctgtc ttaacgcacc atgcacgggc gcttgttatc gaacgtccag
    11821 ccaggaatca gatactgcat tcccatcgca tcgtcacgcg cgcaaagccc gtgtttctga
    11881 tacagctcat gggctttcat cacttgatcc atatcgattt ctacacccag ccccggtttt
    11941 tccggcacct gaaccagacc gcctttgatc tcaaacggtt ctttggtcaa gcgctgattg
    12001 ccttcctgcc aaatccagtg cgtatcaatc gcggtgattt tacccggtgc agcggcggca
    12061 acatgggtaa acatcgccag ggaaatatcg aagtggttgt ttgagtgtga accccaggtc
    12121 aggccaaatt catggcacat ttgcgccaca cgtaccgaac cttgcatcgt ccagaaatgc
    12181 ggatccgcca gcgggatatc aacggattgc agggagagcg tatggcccat ctgccgccag
    12241 tcggtggcga tcatattggt tgcggtcggc aggccagttg cgcgacggaa ctctgccatc
    12301 acttcacgcc cggagaaacc ttgctccgca ccacacggat cttctgcata agccagcgac
    12361 cctttcaggt atttaccaat tttaatcgct tcgttcagcg accaggcacc gttaggatcg
    12421 agcgtaatac gcgcctgcgg gaagcgttgc gccagtgcca caatagactc ggcctcttct
    12481 tccccggcca gtacaccgcc tttcagtttg aaatcgttga agccatattt ttcatacgcc
    12541 gcttccgcca ggcgcaccac cgcatccggc gtcatcgctt cttcatgacg caggcgatac
    12601 cagtcgcatg agtcatccgg ctggctttga tacggcagcg gtgtggcttt gcgatcaccg
    12661 acgaagaaca gataaccgag catttcgact tcgctacgct gttgaccatc gcccagcagg
    12721 gatgccacgt ttacccccag atgctgcccc agcagatcca gcattgccgc ttctatcccg
    12781 gtaactacat gaatagtggt acgcaaatca aatgtctgca aaccgcgccc accagcatca
    12841 cgatcggcaa aagtattacg caccagcgtc agaacgtttt tgtattcacc cagcgtttta
    12901 cctaccacca gcggaatcgc atcttccagc gttttacgga ttttctcgcc gccgggaatt
    12961 tcccctacgc cagtgtgacc agaattatct ttgataatca taatattacg cgtaaagaac
    13021 ggtgcgtgtg caccactcag attcatcagc atactgtcat gacccgccac cgggatgacc
    13081 tgcatttcag taacaacagg cgtcgtaaat tgagaactca tatttatgtc ctttttcaga
    13141 atcaatgacg gccgaaaacg ggacgtttac ggtcaaaggt ccagccgggg atcaggtact
    13201 gcatcggacc tgcgtcgtta cgcgcaccgc caggcagacg tttataggcc tcatgtgcct
    13261 tttgtacctg ttcccagtcc agttccacgc ccagaccggg cgcatcagga acggcaattt
    13321 ttccgttttt aatctccagc ggatttttgg tcaggcgaca atcgccctcc tgccaaatcc
    13381 agtgggtatc gatagcggta ggattacccg gtaccgccgc gcccacatgg gtaaacatcg
    13441 ccagagagat atcgaaatgg ttattagaat ggcagcccca ggtcagcccc cagtcgtcgc
    13501 aaagctgcgc cacacggact gcaccggaaa gcgtccagaa gtgcggatcg gcaagtggaa
    13561 tatctaccgc attgagcatc accgcatgac ccatttcgcg ccagttggtg gcgatcatgt
    13621 tagtcgcgac gggcaagccg gtcgcccgcc gaaactccgc cataacttca cgaccggaga
    13681 aaccctgttc tgcgccgcat ggatcttcgg cataggtaag aacatcattc agccctttgc
    13741 atagagaaat ggcttcatca agtagccagg caccgttggg atcaacggta atccgcgcat
    13801 cagggaagcg tttcttcaat gcacgaacag tgtcgatttc ttgctcgcca ggtaacaccc
    13861 cgcccttaag tttgaaatct ttaaagccat agcgatcctg tgaggcttcc gccagacgca
    13921 caacggcttc gctgttcatc gctttctgat ggcgcaactg ataccactca tggttgcccg
    13981 gcgtattttc cagataagga agatcggttt tggtccgatc accgatataa aacagataac
    14041 cgaggacggt aatagtctcg cgttgcttgc ctggccccaa cagttcgcag accggaacat
    14101 tcagcgcctt acctagcagg tcaagcaagg cggcttccag cgccgccacg gcattaacgc
    14161 gcaattcaaa agtccaggca cctttgccga aggtatcaaa atcggctgcc tgattacctt
    14221 tatgcacctg ctgaaccact ttattcaggc gcgcaacttc ctggcccaga accatcggaa
    14281 tagcatcgac ctgtgtttga taaatcacct ctccgcccgg cgcttcacca atgccggtat
    14341 gcccggcgtt atcggtgagt accacaatat tgcgggtgaa atatgcgtta tgtgccccac
    14401 caatattaag caacatgcta tcgtgcccgg cgaccggaat gactttcata tcagtaataa
    14461 caggactgga ttgtgtcgtc atcttattgt cccgcgacag gtttcagctc gatacgcttg
    14521 atatcgccca ccagcaccag gtagctcagt accgcgatta aggcatgaac accaacataa
    14581 atcagcgccc cattaaagga gccagtcgtg ccaacgatat aaccaattgc gattggcgtg
    14641 acgatgccag aaatgttacc gaacatgttg aacaggccac cggaaagacc gctgatctct
    14701 tttggcgcgg tatctgccat tactgcccaa cccagcgccc cgatgccctt accgaagaag
    14761 gccagcgcca taaagccgat gatcatccac tcaacgttga cgtagttgca gaacaccatc
    14821 accatcgaca gcaacatgcc cattacgatc ggtgttttac gcgcaatgtt cagcgatccc
    14881 gtgcggcgca tcagccagtc ggaaataatc ccacccagca caccgccgat aaaaccgcaa
    14941 accgccggaa cggaagccac aaagcccgct ttcagaatcg acatcccacg cgcctgcacc
    15001 agataaaccg gaaaccaggt aatgaaaaag taagtcaggg cgttgataca gtactgaccg
    15061 atataaacgc cgatcatcat ccgtgacccg agcagttgtt tgatctgccc ccacttcacg
    15121 ctgaacggca ctttaacttt ggtgttttgc tgatccatat tgatcagcgc gccacccgcg
    15181 gcgatgtact ccagctcttt ctgatttacc cccggatgtt gatttggctc gtggatgact
    15241 ttcaaccaga taaagctgat gacaatcccc agactgccca taaagaagaa gacgtgtgac
    15301 cagcccactt catgcgtcag ccagcccata atcggcgcga agatcaccgt tgcgaagtat
    15361 tgagcggagt taaaaatcga caccgccgtt cccctttcct gcgccggaaa ccaggccgca
    15421 acaatgcgac tgttgccggg gaaagatggc gcttcagcaa gcccgaccag gaagcgcaga
    15481 gtaaacaggg caacgataat gccgaatcca ctaaagatat cgacgaagcc ttgcagcaag
    15541 gtaaacatcg accagataaa gatcgaccag aagtagacgc gttttgaacc aaaacgatcc
    15601 agcaaccaac caccagggat ctgcccgata acataagccc atgagaaggc agagaacaca
    15661 tagcccattc ccacgggatc aaggccgata tctttggcca tttccgaacc ggcaatagaa
    15721 agcgtagcgc ggtcgccgta gttgaaggat gtgacgataa acaacatcac cactatccag
    15781 taacgagcat tcgtgcgttt ttccacactg ctcgcagcct gacttaaaga actcgttgtt
    15841 gcactcctga aaattcgcgt tagccacgct cactctggac tgcgacatag ccaggaaatc
    15901 agaggtgacg tagggtgttt tttgccgttt ttataggtcg ttcgccgaat acggcgcgtg
    15961 tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt gcaacaacac
    16021 aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt ggaatccact
    16081 tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat aaagaagagt
    16141 atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta tgaagcagtt
    16201 tttactggca tttgcctgaa aagattcgat tcagcaccgc taaaacgaca tttaccgctc
    16261 gctgaacata tcaggacaac agcgtgcccc actgttcgac ccacggattt gattccgttt
    16321 ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc tgagcgctct
    16381 gttcctgcaa caaggcatcg aactgtttgc cgccattgca gaaatttaca taactgctgt
    16441 cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta tctttgattc
    16501 cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg gacgtaacca
    16561 ccagaacata cttatcctga taaggcagcc agtcgcttaa ttcaggatct tcaaataccg
    16621 ttgctttgtg gccctgtgcg gtcagaattg cttctgcttc ttccgccact aacagcgaat
    16681 ttccgtacat ggtgccgaca aaaataccaa tttccgccat accgttttct ccctggatta
    16741 ggaacttatc tctccatcct gacccgatgg cgcactgaac tcaacccttt cattttcagg
    16801 aagcagaccg cgccagccaa attgtgataa cgcctgcatc caggtgtcgt ccaggcccgc
    16861 gtgaatagtc agcggctcgc cagtaaaagg atgcgtcaat gacaactgac tggcatgtag
    16921 cattaaccgc tggaggccaa aatgctcagc accgctgcga ttctggcgta aatcgccatg
    16981 tttgctatcg ccaataatcg gatgacgcaa atgggcaaga tgtcggcgaa gctgatgttt
    17041 gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt aacgtccggt
    17101 cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg gctgcgggcc
    17161 tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca gtggataatc
    17221 cagcaccgct tcttccatca accagccgcg cacaatcgca tggtaacgtt tctggatttg
    17281 gtgctgttca aactgttgtg ccagcagccg tccggcctcg ctggataatc ccatcaacaa
    17341 cacaccagaa gtgggtcggt ccagacgatg agcagtaaaa acatgctgtc ctatctggtc
    17401 acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctac ggtgaaccag
    17461 ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta tttccagcat
    17521 tagctcgcat catccgcaaa aagagcatcc agtttttcca actcagccag aataagcgcg
    17581 tgttgcggat ggtccgtcgc cagcgccatt tcataatagg gtgcaacagc aaaagcgccc
    17641 ggtaacggct ggttgttatt taacaaatcg tgcattcgcg ggatcagcac ccactgcaac
    17701 cactccagtg gttccatggt gtccataaag aacggttggg tactattaaa ttgatgcggc
    17761 tggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag cgcctgcaac
    17821 tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa actgccgcga
    17881 aggatagcag gaataaaaaa agggagcact gtattcacag cgctcccggt tcgtttcgca
    17941 gcattccagc tacttttgtt gctccctgct catccttgac aacttttcct ctggccttgc
    18001 ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg tgatacttca
    18061 tcctgaagtg tccttggcct tcctgaccag ccgaatcatc ctgaccggtt ctcattctcc
    18121 atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc cagaagcctt
    18181 tccctgaaac accatcctgg tgtgtcctgc agaagtgtca tcatcctgat gttcacttcg
    18241 ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt cgcgaagtct tacaaggcgc
    18301 ttacagacaa tgccttaagg aaaatttcat atgaaagtta agaattaaca atcaacattt
    18361 tgattttaaa tgaaaataac gattcttatc tttttaagta ttcgcttatt tcctggcgat
    18421 ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga ttacagaaga
    18481 ggcttaagtt gattaaggaa ttccgcaaga tttgaggcta aatgcgtccg tttacgtgtt
    18541 cccagcgtct ctttgcacac ttcaccggag agattacaca ccgagatgac ctctaactct
    18601 tcttccagcg tcgcgataaa tagcgtgggc ggcagtttta gccttttctg agtcaccaga
    18661 tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccaggt ttgcagcaat
    18721 gtcagtttga tatagccaaa ctgcgcgtgc atatccccgg caaactgagt cgtataaaag
    18781 gtatgaattg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt tacattttgt
    18841 tccccggtaa acggctgggg ttgccagtat acggcatctt cggtagttga aataatgcac
    18901 ggtgaaggaa caccgtacag ttcctcactt aacggccagc ttttatgctc ttcatgccac
    18961 gcatcgcagt agcgtgcagt aaaatctttc agggcttgtg cggtcaaatc gtccactaat
    19021 ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt atgacggtga
    19081 aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgactcttgg aaaatcaacc
    19141 gattaccggg atacctatga cgccagccta ctgcaaggcg ttccacgtag cctgaatcgc
    19201 gacccgctgg gtctgaaagc ggataacctg ccttttcacg gtacggatat ctggacgctg
    19261 tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctgtcgg tcatgttgaa
    19321 cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta tctcaacagt
    19381 tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga gcgcgactta
    19441 agcacttgcg ctcagggtaa gattagcgtg gcgttatatc gtcttgatga actggaaggc
    19501 cagccgatag gtcattttaa tggcacttgc attgatgacc aggatatcac tatcgataac
    19561 tatgaattca ctactgacta tctggagaat gccacctgtg gtgaaaaagt agtggaagag
    19621 acgcttgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc agattggggt
    19681 tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct gcgttacctg
    19741 gtctcattcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat ctttaatgac
    19801 ctgttacgct tctgccagcc agaaaaattg agcgtttacg cacgttatac ccgtcgtggc
    19861 ggtctggaca ttaacccgtg gcgcagtaat agcgattttg tcccatcgac cacaagactg
    19921 gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag gttgtgaaag
    19981 gtcatcaggc agggctattg taatcaaagg gaatgacgat attcttccca taaggagttt
    20041 ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca gctggaagtg
    20101 gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa ctgttcactt
    20161 gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc tcgttttgaa
    20221 aatttcgata ttaacgtctt gcgccgtgaa cgcggcgtaa agctggaact gattaatccc
    20281 ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa cttgttcgcg
    20341 gtcctgcgtg acattctctt cgtttacggg caaatccata acaccgttcg ttttcccaac
    20401 ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt gcgtaacgct
    20461 cgcgcgctgc atgtgggtga agcgccaaat atggtggtct gctggggcgg tcactcaatt
    20521 aacgaaaacg agtatttgta tgcccgtcgc gtcggaaacc agctgggcct gcgtgagctg
    20581 aatatctgca ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg tgctgcggtc
    20641 ggacacgcgc agcagcgtta caaagacagt cgttttattg gtatgacaga gccgtcgatt
    20701 atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc agatatcgaa
    20761 aaacgtctgg aagcgtttgt ccgtatcgct cacggtatca ttatcttccc tggcggtgtg
    20821 ggtacggcag aagagttgct gtatttgctg ggaattttaa tgaacccggc caacaaagat
    20881 caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt ccgcgtactg
    20941 gacgagtttg tcgtgcatac gctgggtgaa aacgcgcgcc gccattaccg catcatcatt
    21001 gatgacgccg ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt gaaagaaaat
    21061 cgccgcgaca caggcgatgc ctacagcttt aactggtcaa tgcgcattgc gccagatttg
    21121 caaatgccgt ttgagccgtc tcacgagaat atggctaatc tgaagcttta cccggatcaa
    21181 cccgttgaag tgctagctgc cgacctgcgc cgtgcgttct ccggtattgt ggcgggtaac
    21241 gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat caacggcgat
    21301 aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca gcatcgtatg
    21361 aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc tcccctcccc
    21421 cggtgacgtt tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat
    21481 ttgttatatg aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc
    21541 atacaattta tgttgcaacg caaacgtttc cctattttca taaaccgtat tttatcgcta
    21601 tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca
    21661 tatattgcgc gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat
    21721 tctcttttac ttttgattta cagagtaaag cgttgggata atctatcttc caagtagatt
    21781 attgtatttg agatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc
    21841 aaattattac ggcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt
    21901 caggcacctt cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa
    21961 atagatggaa acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg
    22021 caagacagac accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct
    22081 gttcctgcca atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct
    22141 tgcgttcccg atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa
    22201 aaacccgggc gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa
    22261 actgattacc ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc
    22321 aatcaccaat acagttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg
    22381 tgtgattctg tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct tcggtgagca
    22441 gatgatcgtt aaagcgatga gtattctggt gttcccgttt gttggcgtat tgatgctgct
    22501 ggctctgtac ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac
    22561 tgcatctgca accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt
    22621 gttctcgttc aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta
    22681 cggcgatatg gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt
    22741 gctgaccgta atgttcttcg tcttcagctg tgtactgagc ctgactccgg cagacctggc
    22801 tgcggctaaa gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc
    22861 ggttatcgcg tggatagctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg
    22921 ccactacctg ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa
    22981 aggtaagtca atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac
    23041 gacctggatt gttgctaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg
    23101 tccaatcatc gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc
    23161 aatgcgtaag tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc
    23221 aatctccgca atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc
    23281 gcttcctccg ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat
    23341 cggcattggc ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac
    23401 cgacgatctg attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta
    23461 cggctcgctc tctctgaccg gtaaaggcca ccacactgat atcgccatta ttatgggcct
    23521 ggcgggtaac ctgccggata ccgtggatat cgattccatc cctggtttta ttcaggatgt
    23581 gaatactcat ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga
    23641 tcagtgcatg aacttccacg ccgacaacct ttctctgcat gaaaacggta tgcgcattac
    23701 cgcgctggcg ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt
    23761 tatcgttgat gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc
    23821 gtacagttca gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg
    23881 cctgatgatg aaaaacgagc tagcgctgca cagcaaagaa gagctggaac agcacctggc
    23941 gaacgtatgg gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt
    24001 gcctggcaaa ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca
    24061 ggataaaacc accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc
    24121 agtgaacgaa gagaacgctg ctggcggacg cgtggtgact gcgccgacta acggtgcgtg
    24181 cgggattatc ccggcagtgc tggcgtacta cgacaagttt atccgcgaag tgaacgctaa
    24241 ctcactggct cgttacctgc tggtagccag tgccattggt tctctttata agatgaacgc
    24301 gtcaatttct ggtgctgaag tcggctgcca gggtgaagtt ggtgtggcgt gctcaatggc
    24361 ggcggctggt ctggcagaac tgttaggtgc aagcccggcg caggtgtgca tcgcggcgga
    24421 aatcgccatg gagcacaacc tcggtctgac gtgtgacccg gtcgccggac aggtccaggt
    24481 gccatgcatc gagcgtaacg ccattgcggc agtaaaagcg gtgaacgccg cacgtatggc
    24541 gctacgccgt accagcgagc cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga
    24601 aacaggtaaa gatatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcaatgaa
    24661 gatcgttgcc tgcgattaat cgctctccag aggcctcgtt ttgcgaggcc tcttcccgat
    24721 ttctcatcca gccgtagcct gttccggcat cgaatgttac ccttatcgcc tgatctttaa
    24781 gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta ttcgtcgcat
    24841 tcatgccgtt cagggatcgc cctgtgtcga aacctgccag catgcgctcg atcagctcat
    24901 tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg
    24961 ctggcgtcat cagcgtttac cagattacaa atcgggtcga ccgccaatgc cggaagagtt
    25021 gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc
    25081 aaccagcggc aacgaagccg atgacttagc cgccacgctg gcggtcaaag tgacacaggc
    25141 cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac
    25201 attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga
    25261 atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga tcagcagttc
    25321 aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc tggtcgagtt
    25381 tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa
    25441 attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga
    25501 tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc gagccggatg
    25561 caaacgatta tcaacacatt gaatttaatg cacttatata ccggataaga cgcggctagc
    25621 gtcgtatccg gcattatcac atcagcgcat ttaccaggcg gtatggtaaa gctctacaat
    25681 atcctcaagc gttgcttcac gcgggttgcc accggtacaa acatcatcca gtgccgcctg
    25741 cgccagtgcc ggaatgtctt ccttgcgtac accaacatca cgcaaatgtg gcggaatacc
    25801 gacatcacgg ttgagagcaa acaccgcttc aacagcggca ttacgcgcct cttccagcca
    25861 ctttcacgcc cataacgcgc gcgatatcgc ggtacttctc accggtaaag tcagcgttat
    25921 agcgcatgac atgcggtaac aggatggcgt tcgcaacacc gtgtggagtg ttgtaaaacg
    25981 cgcccagtgg atgcgccata ccatgcacca accctaaccc aacattcgag aagcccatac
    26041 ccgcaacata ctgcccgagc gccatttctt ctccggcatc cttatcacca gcaaccgatc
    26101 ctcgcagcgc cccagcaatg atttcaatcg ctttaatgtg cagtgcatcg gttagcgccc
    26161 acgcgccacg ggtaatatac ccctcaatag catgagtgag cgcatcgaca cccgtcgcag
    26221 ctttcagcgc tggaggcata ccatccatca tgtcagtgtc aataaacgcc acctgcggga
    26281 tatcatgcgg atcaacgcaa acaaacttgc gccgtttctc ttcgtcagtg atcacgtagt
    26341 taatggtcac ttctgccgca gtacctgctg tggtaggaat tgccagaatc ggtacactgg
    26401 gtttattggt cggggaaagc ccttccaggc tacgcacatc ggcaaactcc gggttgttgc
    26461 tgataatgcc aatcgcttta caagtatcct gtggagaacc accaccaata gcgatcaggt
    26521 aatccgcgcc gctattctgg aatacaccga gcccttcttt gacgacagta attgttgggt
    26581 tgggcactac gccgtcgtaa atcgcccatg ccagccctgc agcatccatc ttatcggtca
    26641 ctttcgccac cacgccgcat tgcaccagcg ttttatcggt gacgatcagc gccttctgat
    26701 aaccacggcg tttcacctca tcggttaaag ccccaacagc accccgacca aaccatgccg
    26761 tttcgttcag aatcattctg ttagccatca tccttctcct tgttgcttta cgaaattact
    26821 cttcaatccg taacccatag gttttgaatt tctccagcac tacggcaatc tcttcatcgc
    26881 tcagcactgg caccgggtcc gtaatcgcca gggtcgtcag gtaaagttgc gccagcactt
    26941 caacttcatg cgccagccat aacgcttttt ccagattcac ctcacaagcg ataagcccat
    27001 gatgttgtaa caaagttgcc ttacgatttt tgagagccag cgcaacatgt tcagaaagtt
    27061 cgcgtgttcc aaaggtcgca taaggcgcgc aaggaataga attaccacct gccgccgcaa
    27121 tcatgtagtg aatagcgggg atcggtcggt taagaatgga aactgctgtg caatgaacgg
    27181 catgattgtg aacaaccgcg ttggcatccg gtctgctttg ataggccgcc atatggaaac
    27241 gccattcgct tgagggga
//
LOCUS       DAJTMK010000039        26772 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTMK010000039 DAJTMK010000000
VERSION     DAJTMK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26772
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.040"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..328)
                     /gene="rpnB"
                     /locus_tag="OO135_001415"
     CDS             complement(<1..328)
                     /gene="rpnB"
                     /locus_tag="OO135_001415"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5397323.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
     gene            complement(525..1298)
                     /gene="hisP"
                     /locus_tag="OO135_001416"
     CDS             complement(525..1298)
                     /gene="hisP"
                     /locus_tag="OO135_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5397324.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1306..2022)
                     /locus_tag="OO135_001417"
     CDS             complement(1306..2022)
                     /locus_tag="OO135_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5397325.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2019..2705)
                     /gene="hisQ"
                     /locus_tag="OO135_001418"
     CDS             complement(2019..2705)
                     /gene="hisQ"
                     /locus_tag="OO135_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5397326.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2795..3577)
                     /gene="hisJ"
                     /locus_tag="OO135_001419"
     CDS             complement(2795..3577)
                     /gene="hisJ"
                     /locus_tag="OO135_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5397327.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3798..4580)
                     /gene="argT"
                     /locus_tag="OO135_001420"
     CDS             complement(3798..4580)
                     /gene="argT"
                     /locus_tag="OO135_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5397328.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKCQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4846..5415)
                     /gene="ubiX"
                     /locus_tag="OO135_001421"
     CDS             complement(4846..5415)
                     /gene="ubiX"
                     /locus_tag="OO135_001421"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5397329.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(5510..7027)
                     /gene="purF"
                     /locus_tag="OO135_001422"
     CDS             complement(5510..7027)
                     /gene="purF"
                     /locus_tag="OO135_001422"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5397330.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7064..7552)
                     /gene="cvpA"
                     /locus_tag="OO135_001423"
     CDS             complement(7064..7552)
                     /gene="cvpA"
                     /locus_tag="OO135_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5397331.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     GA"
     gene            complement(7812..8474)
                     /gene="dedD"
                     /locus_tag="OO135_001424"
     CDS             complement(7812..8474)
                     /gene="dedD"
                     /locus_tag="OO135_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5397332.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8464..9732)
                     /gene="folC"
                     /locus_tag="OO135_001425"
     CDS             complement(8464..9732)
                     /gene="folC"
                     /locus_tag="OO135_001425"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5397333.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALHASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9802..10716)
                     /gene="accD"
                     /locus_tag="OO135_001426"
     CDS             complement(9802..10716)
                     /gene="accD"
                     /locus_tag="OO135_001426"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5397334.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10872..11531)
                     /locus_tag="OO135_001427"
     CDS             complement(10872..11531)
                     /locus_tag="OO135_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5397335.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11614..12426)
                     /gene="truA"
                     /locus_tag="OO135_001428"
     CDS             complement(11614..12426)
                     /gene="truA"
                     /locus_tag="OO135_001428"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5397336.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTVPRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12426..13439)
                     /locus_tag="OO135_001429"
     CDS             complement(12426..13439)
                     /locus_tag="OO135_001429"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5397337.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13505..14641)
                     /gene="pdxB"
                     /locus_tag="OO135_001430"
     CDS             complement(13505..14641)
                     /gene="pdxB"
                     /locus_tag="OO135_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5397338.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14740..15735
                     /gene="flk"
                     /locus_tag="OO135_001431"
     CDS             14740..15735
                     /gene="flk"
                     /locus_tag="OO135_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5397339.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15732..16910)
                     /locus_tag="OO135_001432"
     CDS             complement(15732..16910)
                     /locus_tag="OO135_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5397340.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17194..18414)
                     /gene="fabB"
                     /locus_tag="OO135_001433"
     CDS             complement(17194..18414)
                     /gene="fabB"
                     /locus_tag="OO135_001433"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5397341.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDARRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18573..20579
                     /gene="mnmC"
                     /locus_tag="OO135_001434"
     CDS             18573..20579
                     /gene="mnmC"
                     /locus_tag="OO135_001434"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5397342.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20700..20978)
                     /locus_tag="OO135_001435"
     CDS             complement(20700..20978)
                     /locus_tag="OO135_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5397343.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21012..21560)
                     /gene="epmC"
                     /locus_tag="OO135_001436"
     CDS             complement(21012..21560)
                     /gene="epmC"
                     /locus_tag="OO135_001436"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5397344.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21560..22369)
                     /locus_tag="OO135_001437"
     CDS             complement(21560..22369)
                     /locus_tag="OO135_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5397345.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22369..23193)
                     /gene="mepA"
                     /locus_tag="OO135_001438"
     CDS             complement(22369..23193)
                     /gene="mepA"
                     /locus_tag="OO135_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5397346.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23197..24282)
                     /gene="aroC"
                     /locus_tag="OO135_001439"
     CDS             complement(23197..24282)
                     /gene="aroC"
                     /locus_tag="OO135_001439"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5397347.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24317..25249)
                     /gene="prmB"
                     /locus_tag="OO135_001440"
     CDS             complement(24317..25249)
                     /gene="prmB"
                     /locus_tag="OO135_001440"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5397348.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25415..25966
                     /gene="smrB"
                     /locus_tag="OO135_001441"
     CDS             25415..25966
                     /gene="smrB"
                     /locus_tag="OO135_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5397349.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRHEHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEVWLPPELP"
     gene            complement(26039..26752)
                     /locus_tag="OO135_001442"
     CDS             complement(26039..26752)
                     /locus_tag="OO135_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5397350.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6595 a         7321 c         6761 g         6095 t
ORIGIN      
        1 ccgcatcaag atggttttgc attgccgcaa tggaataacg catcatgcga aaagccatta
       61 attcttccgg cttactttgg tgctctatca ctacataaat ataacccact ccctcctgcg
      121 ttttcacaga ccacaagagg tcggaataat attgccgcag gtcttcatca ataaaactgt
      181 ttggttccag tttaagcgtc gttaaatcac acaatttgcg cagcggcgcg ggaagatgaa
      241 tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa agatttaaat accgcatcat
      301 gcggcgtgga agttgtcgat attgtcatgg cgttccgtca cccctcaaat ttaagatgac
      361 gcgacaataa ccacgattaa cacccaatgc agcaggcagt ttttatcttt ggacagcgaa
      421 ttcagaagta tttttagcgg actgcaactt ttacatttca gcggaagctg actcgcaaat
      481 tccccatgaa aatactccgg cagcgttagc caccggagag gaaattattt cagcgatccc
      541 ttaaggaact gttgcagacg agggctttgc gggttgccaa ataactgctc cggcgcgccc
      601 tcttcttcta ttttcccctg atggaggaaa atgacatgag tagaaacatg gcgagcaaag
      661 cccatttcgt gagtcactac caccatggtt ttcccctctt ctgccagttg ctgcataata
      721 cgcaacactt cgcctaccag ttcaggatcg agcgccgagg taggttcatc aaacagtaaa
      781 acttccggtt ccatcgccag cgcccgcgcg atagaaacac gctgttgctg accgccggaa
      841 agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc cgacttttgc cagatacttg
      901 accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca cctgaatcgg cgcttccatg
      961 acgttttcca gcaccgtcat atggctccag agattgaagt gctggaatac catcgtcagg
     1021 cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt tgagttgacc gtctttgtcg
     1081 cgcaccagat tgatcgtctg gccattgacc acgatcgacc cttcactcgg tttttcgagg
     1141 aagttaatgc agcgcagaaa ggtacttttc cccgatcccg acgatccgat gatgcttatt
     1201 acatctccgg cattcgcttg cagtgagacc cctttcagca cttcatgttc gccgtagcgt
     1261 ttgtgcaaat cgataacgtt taatttattc tcggacatcg tattctcagt gcgttgaaga
     1321 aggtttcaca tgctgcaacc agcgtttttc cgctctgcga aagaggctga tcaggacata
     1381 agagatgatt aaatagagca ccgcggcaat gccgaaggcg gtaaaaggtt gatacgtggc
     1441 ggcgttaata tcgcgggcta ttttcagcag atccggcaca gtggccgtaa acgccagcgc
     1501 agtagagtgc agcatcagga tcacttcgtt gctgtatgcc ggtaacgcaa tacgcagcgc
     1561 agaaggcaaa ataatgcaac gatacatttt aaaagtcgag aagccatagg ctctggcggc
     1621 ttcaatttcc ccatgcggaa ccgaacggat tgccccagca aaaatctcgg tagtgtaagc
     1681 gcaggtgtta agcgtcagcg ccagcacggt acagttcagg ccactgcgga agaaagcgtt
     1741 aaggaattcg gttcccttaa caatctcaag cgtgtacatg ccggaataga acaccagcaa
     1801 ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat aaccagattg gaaactggat
     1861 gtatttatta ctggagacac gaccaatcgc cagaaacagc gccaggactc cgcctatcac
     1921 taccgacaaa ataagcagcc acagagtgat cgccacacca gtaaagcgat aaccgtcggt
     1981 ccacagcagc ggtttccagt attcatgtaa gatttcgatc acaggtcagc cctcttcaca
     2041 cccacggagt agcggcgctc aaggaacagc agcacaccat tggaaacagt ggtgaaaacc
     2101 aggtaaatca cgccacagac gatggcgaaa tagaacggtt cccaggtact tttgcctgcc
     2161 agttgggtgg ctttgaccac atcttccagg ccgagtaacg aaaccagtgc ggtagatttg
     2221 aggatcacct gccagttgtt gccaatgcct ggcagggcgt aacgcatcat cgccggaaac
     2281 atgatccgcc gaaacacttg cccacgagta aaaccgaacg ccgtcgccgc ctctatatgt
     2341 cctttcggca ctgccataaa agcaccacgg aacgtttcgg taaaataagc accgtagata
     2401 aaaccgagag tgataatacc agcgaccatc ggatcgatat caatctgccc gacgcccatc
     2461 gcctccgtca ccgtgtttag cgcaatctgc aaaccgaaga aaatcagcag catcaacact
     2521 aagtccggca cgccacgaat cagcgtggtg tacccttcga aaatcagccc cgaaagccga
     2581 ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga ttacagcgag cactacagag
     2641 ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa taacacctga aaacccatac
     2701 aacatgctgc ctgtcctgtc gtgtgtggtg aattaccgcc tcttgtctcc ctccggttac
     2761 ccgaagggag cgggctttct ttcaccgatg gcgattagcc accataaaca tcaaaatcga
     2821 agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg catttcggca aaggctttgt
     2881 tcagtgcttc gcgcagttcg ttatcttctt tacgcaggcc catgccggtc cctacgccaa
     2941 acagtttttc atctttaaca gacgggcctc cgaatttgta atctttaccg acaggttgtt
     3001 tgaggaaacc ttcgctggca gcgacctcat cctggaacgc ggcatcaata cgtccggcag
     3061 tcaggtcaga ataaatgttg tcctgcccct gatacgagac gatttcaatg ccttttggtg
     3121 cccaatgttc attaccgaac gtctcctggg tggtgccctg caatacgcct acccgtttgc
     3181 ctttcagcga ctcgactgtc ggctgaatgt cagaattttt cgccaccacc aaacgagaat
     3241 cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg acgtttttcc gtaatggaaa
     3301 gcgatgacat gatggcgtca atcttcttcg cttttaagga cgggattaac gcatccagcg
     3361 gattttcgac aaacgtacat tgcgtattga tgcgtttgca taattccttt gccagatcga
     3421 tatcgaagcc aaccagttcg ccttgtgaat tttttgattc aaatggcgca taggtcgggt
     3481 cggtaccgat gcggatgttt tgcggaatcg cagcaaatgc cgcagttgcg ctggagaagg
     3541 ccagaaccag agagagcgat agcaccagtt ttttcataat gtcctcaact gacagtcttt
     3601 ttatagggaa tatttacagg tttgttacac ttatcgtgcc ataaaattga ccatcaaggc
     3661 aaaatatccc gccccgttag acgtattatt tttcattaaa aatgcttaag tatgcatttc
     3721 aatgcaccac gatagtgcaa caaacctagc gtgcaccatg atgatcattc catcaggtac
     3781 agcttcccag cgacgtatca gtcaccgtag acattaaagt cgaaatactt tttcgccatc
     3841 ttgtcgtagg tgccgtcctg acgcagctcg ccaagcgcct tattgaaggc agccgtcagt
     3901 tcagcatcat ctttacgtag ccctacaccg gtgccatcac cgaagtattt tttgtctttt
     3961 actgatgagc cagcaaaggc gaaatcttta ccagcaggtt gcttgaggaa tccttcgctg
     4021 gcagcaactt catcttgtaa cgcagcatcc agacgtcctg cagccagatc ggaatagacc
     4081 aaatcctggt tggcataggc caccacatcc acgcctttgc tacgccaggt ctcgttagcg
     4141 taagcttcct gggttgaccc ctgcagcaca ccaacatgtt tacctttcag tgaatccagc
     4201 gttggctgaa tcggtgaacc tttggcagca atcaaacgag aatctgcggc gtacagcttg
     4261 tcggagaagg caatctcctg ctgacattta tcggtaatgg aaagcgacga aataatggcg
     4321 tcgatttttt tcgctttcag tgaggggatc agcgcgtcaa agtcactggc aacccaggta
     4381 catttcacct gcatccgttt gcacatctcg ttaccgagat cgatatcaaa gccaacaaaa
     4441 tcacctttag catctttcga cgagaacggc gcataggtgg tatcggttcc gatacgtacc
     4501 gtctccggta gcgccgcata gctggaagcc gctgcggaga gaccgactaa caaagacaga
     4561 gcgagaatcg acttcttcat acataaccct caagtgaaaa tggctttttt atgttgtgta
     4621 ttgtgttgtg tgtttgcagg ctcttttatg caggtcttat gccatcttga cgacaacagt
     4681 aacattcaac gttaaatatg ttaataagac gttgcattat tgtcctgaag ttgaagatag
     4741 caggtatagc ggttgaatcg cagcgtttcg attgaatggc agaaacaaaa tgtcgaggat
     4801 ttgatcgcgg ttgcaaaatt gccctgaaac agggcaacag cggagttatg cgccctgcca
     4861 gcgggcaaag agatcttcag gaagggttat tgcaaactgg tcaagaacac gattaaccgt
     4921 ctgatttatc acatcatcaa gggattgcgg gcgatgataa aacgccggaa caggaggcat
     4981 aatcaccgca ccgatttctg ccgcctgagt cattaaacgc agatggccta agtgcaatgg
     5041 tgtttcacgc acgcagagca ccaacgggcg acgctctttc agcaccacat ctgccgcacg
     5101 ggtcagtaag ccatcagtat agctatggac aatgccggaa agggttttga ttgaacaggg
     5161 taaaatcacc atccccagcg tctggaaaga accggaagag atgctggcgg aaatatcgcg
     5221 cgcatcgtgc gtgacatcag ctaatgcctg cacttcgcgc agggaaaaat ccgtttcgag
     5281 ggataaggtc tggcgcgccg cctggctcat caccagatgc gtttcgatat ctgtgacatc
     5341 gcgcagaacc tgtaataagc gcacgccata aatcgcgccg ctggcaccgc tgatgcctac
     5401 aatgagtcgt ttcataaata gttgccctgt cagacttgca ggcagacttt gcagtatttc
     5461 ggcgggagtt gcaagtcagg gtgccagacc ggcaccctca gcgaaggcat catccttcgt
     5521 tatgcatttc gagattttcc acttcgttct gacgttgcac tgctttggcg tcatcattac
     5581 gtaacgtatc gaggaaatcg aggtagccct gatcaacatc tttggtgacg tagacgccgt
     5641 tgaacaccga gcattcaaac tgctggatat ccggattttc agcgcgaacg gcgtcgatca
     5701 gatcgttcag atcctggaaa atcaacccgt cagcaccgat gatctggcga atttcatcaa
     5761 cttcgcgacc gtgagcgatc agttccgtgg cgctcggcat atcaatacca taaacgttcg
     5821 ggaagcgaat ttccggtgcc gcagaagcga ggtacacttt cttcgctccg gcttcgcgtg
     5881 ccatctcgat aatctgctca gaagtggtgc cacggacgat ggagtcgtcg accagcagga
     5941 cgtttttatc gcggaactcg gcgcggttgg cgttcagttt acggcgcacg gacttacgac
     6001 gcagctgctg gcccggcatg atgaaggtgc ggccaacata gcggttttta acgaagccct
     6061 ggcggtacgg cttgcccaga atacgagcaa tttccagcgc gatatcacac gaggtttccg
     6121 ggatcgggat caccacgtcg atatccagat cttcccattc gcgggcaatt ttctcaccca
     6181 gtttcgtgcc catattcaca cgcgcgctgt aaacggaaat tttgtcgata aacgagtccg
     6241 ggcgggcaaa gtatacatac tcaaacaggc acggattgct gaccggattg tcagcacatt
     6301 gacgggtaaa caactgccct tcttcagtga tgtaaatcgc ttcgcccggc gcgacgtcac
     6361 gcaggaaatc aaagcccagc gtatcgagcg ctacgctttc ggaagcgacc atatattctg
     6421 tacggttctc gtcaatatca cgttttccca gtaccagcgg acgaatcccg tttggatcgc
     6481 ggaaagcaac cataccgtgg ccgataatca tcgccacaca ggcatatgcg ccgcggatta
     6541 agcggtttgt ggcagcaatg gcagcgaaaa tattgtcggc ttccagcggg tagtggcgga
     6601 agttgtccag ctcgctagcg aagatattaa gcagaatttc cgagtcggaa gtggtgttga
     6661 tgtggcggcg tttttcttca aacagttttt tacgcaactc gtgagcgttg gtcagattgc
     6721 cgttgtgggc aagcgtaatg ccatacgggg agttaacgta aaacggctgc gcttcagagg
     6781 cgctggagct gccagccgta gggtaacgca catgaccaat gcccatattg ccctgcaaac
     6841 gctgcatatg gcgagcttca aatacatcgc tcaccagccc gttcgcttta cgcaaacgga
     6901 agcagttatt ggcatctatg gtgatgatgc cggcggcatc ctgaccgcga tgctgaagca
     6961 ccgttaaggc atcataaatc gactggttaa ccggcataac accggcgata ccgacaatac
     7021 cgcacatacg tctttttcct cgttaagcca catctcagag cacttacgct ccgggcaaga
     7081 aacttgacga gctttgcaga taatcaaaaa accatctgat gataaaactg aactgcggga
     7141 tcagctgtga tttgctccag tcttcgcttt tcgacacccc ggtaaaggag tcgagaaaga
     7201 agagaatggc ggcaacaatc aacacaccgc gcaacgcacc gaaacagacg cccagcaccc
     7261 gatcggtgcc tgacaacccc gttttctcca ccaactggcc tatcacgaag ttcacgattg
     7321 caccaacgat cagggtagcg ataaacagta ccgcgatggc aattccattt cgaaccagtt
     7381 cgtcttcaaa gcccgtaaac cagactgaca ggtaagtgta gtaatgactg gcaacaaaga
     7441 aagcacaacc ccatgtcacc agcgataacg cttcacgaac aaagccgcgg atcaggctaa
     7501 ccagagagga aaaagcaatc accgcgatta tggcgtaatc aatccagacc atatgtgtcc
     7561 cacgatttta cgccctgtca tcctgttcgg ggcgcattct aacagaaaaa ggaaacgttt
     7621 gcgtagggat ttccttccca cgcatcaata aaaatgggcg ctgaaaaaat attcaacgcc
     7681 atcgactttt tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc gtaaacgcct
     7741 tatccgacct acggttctaa cactgcccag gcctgataag acgcgccagc gtcgcatcag
     7801 gcaagactgt attaattcgg cgtatagccc attaccacgc cacttaagcc agaaagttgc
     7861 ttcaactcac ccagcgaacc tttcagctta tctttcgagg catccggccc aaccagaata
     7921 cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata accggcaccg
     7981 cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcacc cagttgcaca
     8041 acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg ttctggcttc
     8101 ggcgcaggtg gtgcttcaac cttcggttta ggcggctcca ccggtttcgg cttcggtggg
     8161 gcgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg
     8221 agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc cggcggctgc
     8281 gtcggcaacg cctgggtggc ggctggcatc atatcaggct catcgcgatc gcccgctttt
     8341 ggaaccagcg ggatagccgc aaactcatcc tgataatgtt ttttctgccc gtccagcagc
     8401 cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa ccgattctga
     8461 aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg
     8521 gaaagaacca cacaccagca cggtgtcttc cgctttagcg tccgccattg cggcatccca
     8581 tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca gttgttctgc
     8641 cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca cgcttttcaa
     8701 ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg ccagcacgcg
     8761 cccgtttttc ggtagcgctt tcatacgccc ggtgagatat tccgccgcat gtggattatg
     8821 cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat
     8881 tgcgctggca atcccgtcgc gaatggcatt ttcactgact tccagcccgc tggcatgcag
     8941 tgccgccagc gctgttgcgg cattcggttg cgggacaagc ggcaacggca gattttccag
     9001 cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt tccactcaac
     9061 gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg tagaaggcat
     9121 ttccggctca ccgacaattg ccggttttgc gctgcgaaag atgcctgctt tctcgcgacc
     9181 aatactttcg cgatctggcc ccagccagtc ggtatgatcc agcgcaatac tggttactac
     9241 cgcgacatcg gcatcgacaa tattggttgc gtccagacga ccgcccagcc ctacttccag
     9301 aatcaccacg tcaagttgtg cctgtttgaa cagccacaac gccgacagcg taccgtactc
     9361 gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg
     9421 ggccgattcc ggcaattcct gaccctgcac acgtacgcgc tcggtataac gcaccagatg
     9481 cggcgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact ccagcgtacg
     9541 gcaggtggtg cctttgccat tcgtacccgc aacggtaaac acaaatggcg ctggtttcag
     9601 aacgccaaga cgagccgcga cctggctcac gcgctcaagg ccgagatcga tagttttact
     9661 gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt gaggagtgcg
     9721 tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg gcagagccag
     9781 tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg ggggtaccac
     9841 tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact tcgccagaat
     9901 gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg cgcctttctc
     9961 gatcaggaat tcactgcgct ggaatcctag cggcagtttt tcgcgaacgg tctgttcgat
    10021 aacacgcgga ccggcaaagc caattaacgc tttcggttca gcgatgttga gatcgcccag
    10081 catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg
    10141 caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga
    10201 catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca gcgggcagtt
    10261 atcttccagc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag accccattga
    10321 accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat acagagtgcc
    10381 tttcatcacc accagcgcat ctttttcgcc ggtttctttc tgcgcagaag ccagacggtc
    10441 tttatacttc ttggagtcac gaaacttcag cacatctttc ggctcaagct cgctacccag
    10501 ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg tcatacgcat
    10561 gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc ggtataaaac
    10621 ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct tgcgggtggg
    10681 agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg tctgaacctg
    10741 gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc aacagaccat
    10801 gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg gtcgaaccgc
    10861 ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacgaat aaattcgaaa
    10921 accccaggca atattgaaac aaaaataatg ccaacaatca tcagcttaag gttgctctga
    10981 atgaagggga gcgtgccgaa gaaataaccc gcgtaggtaa aaatcagtac ccacaacagt
    11041 gcgccgatca cgttataagc ggcgaaatga cggtacgaca tgtggcccat tcccgcaaca
    11101 aacggcgcga acgttctgac gatcggtaca aaacgggcga gaataatcgt tttgccgcca
    11161 tgtctctcgt aaaactgatg agtcttatca agataactgc aacgaaagat tttcgaatcc
    11221 gggttactga acagtttgtc accaaataac cgtccaatgg tatagttcac tgcatcaccg
    11281 acaatagcgg caatcaacat aagcaccacc atcatatgga cattgagatc gttagtttcc
    11341 agcgacgcca atgcaccagc aacaaacagc agcgaatcac caggcaggaa tggcgttacc
    11401 accagccccg tttcgcagaa cagaatcaaa aacagaatgg cataaaccca gacgccgtac
    11461 tccgcgacca gctctgccag atgcacgtca atgtgcagga taaaatcgat gaggaaataa
    11521 atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta actacgttgg
    11581 ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata gcgggcccat
    11641 tggcggtttt gggagatcat accggtcagg gtaatccacc gcgaccagat acagcccttc
    11701 cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact ctgctatcca
    11761 gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat tcctgaccat
    11821 atgatgtaca aaggcattcg ctttgatatc taccaccaca taaggaccgt gacgcgtgac
    11881 attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg cacggaacga
    11941 ggtgaaatca ttctcgccca gcaagcattg cgcagcccga tgcatccgtt cagcgtccag
    12001 cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc gatgattgta
    12061 gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat catcaggtac
    12121 agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc ccaaggtcca
    12181 cgccgcgtct ttgcgcggca cggttgtttc gaaatgcacc acctgcccgg taccgtgtac
    12241 ccctgcgtca gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca cctgggagag
    12301 cgccttttcc agcttctcct gcacactgcg gacttcattc tgtcgttgcc agccgtaata
    12361 cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcagtt gttgctggtc
    12421 ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc catcagcgcg
    12481 ccgccaaagc gaacgttatc ggccaccgac cagaactgga cttgctccgg cataccgtag
    12541 tcattacgca cgcagccaac agaaagatgc ggcgtacccg aagcatcccc tacctgagtc
    12601 gggaattcgt tctcttcaga gagcacaata tcttcgccct gagcaaacgc atcacgcgct
    12661 tcttctgccg ccagcggacg cagtgcttca aagttgacca tctgggcatg accgtagaat
    12721 accggtgcct ggacgacgct tgccgaaatc atcagccctt cgtcctgcag gattttgcgt
    12781 acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg caacaacggc
    12841 agcatattga acgccagttg acgaccgaag aaatcttctt cgtcaatcgg aatgccgttg
    12901 agcaatttcg cactctgccc cgctaacgca tcgaccgctt ttttgccctg ggcggaggct
    12961 gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa cggtttcagt
    13021 gccgccagca gctggctggt cagactgtct ggtacggcga tgacattccg gttgcggtaa
    13081 tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg ttcgagagca
    13141 aacagtccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc aacccaggca
    13201 gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca
    13261 gcggcatcct gcacggtgat tgttttacca ccaaagcgca gttgttcgcc tgcgctttcg
    13321 ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag cgtttcaagc
    13381 agggcttcgc ccacagcgcc agttgcgccc aggacggcaa tgttccagcc ttcagacatg
    13441 gtggtttact ccagaaatag caaagctccc tgccaatgtt acagcagaga gcatgaagaa
    13501 gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc aatgatgccg
    13561 cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca agatagtttt
    13621 tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc
    13681 ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg agcgggccat
    13741 gcagcgtaat gcgaccaaac tctggcgcgg gcagtaatgt atccagcgca acgtgctgtt
    13801 catgcccaat aaacttgcta taggcttcaa acacttgcgt agtaccgcgt gctttacctt
    13861 ccagggtata acctgcgata tgcggcgtgc cgatatccac ttttgtcagc aactccacgt
    13921 taagttccgg ttcgccttcc cagacatcca gcactacgct taacttctgg ccttcattca
    13981 ggcaggtcag caacgcagta ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg
    14041 ctcccggctt caggctacgg atcagttttt catccgccag atgcagcgtt ttgtacggcc
    14101 catctttgaa gagtggcgta tggaaagtca gaatatccgc atgctgaact aattcatcca
    14161 gcgagcggaa atcaccctca tccccacggt cagcgcgagg cggatcgcaa agcaatgttt
    14221 taatccctaa cgcttccagt cgcgcctgca atcgacgtcc aacgttacca acgcccacga
    14281 tccccaccgt acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc agtagtgagg
    14341 agaaaacata ttccaccacc gcaatcgcat tacagccagg tgcagcggaa aaaccaattc
    14401 ccgcctgctt caaccatgct tcatcgacat ggtcggtccc cgcagtggca gtgccaacaa
    14461 atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa cgcaccatca
    14521 gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc gcggtcacct
    14581 cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca aggattttca
    14641 cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata atctcgcggc
    14701 caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc tatttccggc
    14761 cctcctcctg ggcaaccacc aggtcaggga gataacctgc cgtctggcgc gggcaatcag
    14821 cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt gacctcactg
    14881 acgcaacagc aaagagcaga actgtgggcg ggtatcaggc acgatattgg tctgtcggga
    14941 gattcaccgc tgctttcgcg tcacttccct gccgctgagc ataatctggc gcaacgtctg
    15001 ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg ggagtattta
    15061 cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt tggtcagacg
    15121 ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg
    15181 aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg accgtccttt attaccggcg
    15241 gagcacaatg cgctaaaaca gctggtgacc aaacttgcgg cggcaacggg ggaacccagc
    15301 aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga gttaattcca
    15361 gcgaaactgt ttaaccatct ggtgacctgg ctacaggcgc gtcagacgct aagccagcaa
    15421 aatacgccga cgctggaatc actacagatg gcgctaaaac aacctttaga tgccagtgaa
    15481 ctggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca atcatcgctt
    15541 tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt taaagggatt
    15601 gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga cacgttgcaa
    15661 aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct gatgctggtc
    15721 tggctggttc gttaaccccg acgaaatgac agtatcgtga caataatacc cagcaccgca
    15781 gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag cattcccgcc
    15841 agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg
    15901 ccgcgaactt gtgaggggac gcgtttaacc acctccacgc ccagcgcagg aaagataagc
    15961 gaacatccgg ctccggttaa cgccgcgccc gctaatgcga cccatgcacc tggggcttgc
    16021 cagagcagca acaagcccac cgtttctaca agcagagaga caatcgccac tttcacgccg
    16081 ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc gccgccaaac
    16141 gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag cgaaacgaaa
    16201 gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg tttccagata
    16261 agcccgacaa cgctccacag cgatggacgt tctcccgcca gggccggtac tttgcgcact
    16321 gtgccgttac aggcccacgc cagtaagggt aataccattg tggtgatcgc cagtgcggca
    16381 aaaccgtaat ggctatgaat caacaggcca agcggcgcac caacagcgag ggcaccgtaa
    16441 atcgccattc cgttccatga catcactttg ccagagtgtt ttgcccctac gatgcctaat
    16501 ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa agccaagaat caaacgcccg
    16561 acgaccaaca gggcaaattt caacggtgcg gagacaggca aaatcgccgc cagcagcaac
    16621 gcgccgccag ccagaccaca agctaacatc ccctgaagcg ccgaacgttt tgcaccatat
    16681 tgatcggcca gtcgcccggc atagccacgc gtcagcaccg tagccagaaa ctgaatcccg
    16741 acggcaatcc cgaccatggt attgccatag cccagatcat gatgaacaaa cagcgggata
    16801 accggcaacg gcagccctac ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag
    16861 agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta cagcagtcat gcattactcc
    16921 agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca gggttacagt
    16981 gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt
    17041 gcctggaact ggcggcgatt gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg
    17101 cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc
    17161 cgacctactc cgactactcc gacctactgc gaattaatct ttcagcttgc gcattaccag
    17221 cgtggcgttg gtgccgccga agccgaagct gttagacata acggtggtca gttcgcgatc
    17281 ggtcgtttcg gtcacgatgt tcagacctgc cgcctgctcg tccagctctt caatgttgat
    17341 gctcggggcg ataaagccgt gttccagcat cagcagagag tagatagctt cctgtacgcc
    17401 agcagcgccc agagagtgac cggtcatggc tttggttgca gaaatcgccg ggctcttatc
    17461 gccgaacact tcacggatag ctgccagttc tttcacgtcg ccaaccggag tcgaagtacc
    17521 atgagagttc aggtaatcga ttggggtatc aacgccgtgc atcgccatct tcatgcagcg
    17581 tactgcgcct tcgccagacg gagcaaccat gtctgcacca tcagaggttg cgccgtagcc
    17641 aacgatttca gcatagatgt gagcaccacg cgccagcgcg tgttccagct cttcaaccac
    17701 taccataccg ccgccgccag cgataacgaa accgtcacgg cgagcgtcgt aagtacggga
    17761 ggctttttcc ggggtgtcgt tgtatttagt agacagcgca cccatcgcgt cgaattcgca
    17821 ggccatttcc cagcacagct cttcgccgcc gccagcaaac acgatgtcct gtttgcccag
    17881 ttggatctgc tctactgcgt taccgataca gtgtgcggaa gtcgcacacg cggagctgat
    17941 ggagtagtta acgccatgaa ttttaaacgg cgtggcgagg caggcagaaa cgccggatgc
    18001 catcgctttg gtgaccacat acgggccaac cgctttcagg ccgcgcgggc cgcgcattgc
    18061 gtcagcgccg aatacctgga aacgcgggga accgccgcca gaacctgcaa tcaggccaac
    18121 gcgcgggtta ttctggtaag cttccggaga aaggcccgca tctgcgattg cctgctccat
    18181 agaaaggaat gcataaatgg atgcgtcgct cataaagcgc acaactttgc ggtcaatgag
    18241 gccagtggta tccagtttta cgttgcccca gacgtggcta cgcatgccgg aatccttcag
    18301 ctcctgagag aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga cttcctgctg
    18361 gttattaccg atgctggaaa caatgcccag gccagtaatc actgcacgtt tcattcaata
    18421 cctctgtaag tcgcacatag agtaagtttc gaatgcacaa tagcgtacac ttgtacgccg
    18481 aacaagtccg atcagccatt taatagagaa atttgcgcag ccttacacac atcgctaaga
    18541 tcgagccacc gcctgtaaga cgagtaactt acgtgaaaca ctactccata caacctgcca
    18601 acctcgaatt taatgctgag ggtacacctg tttcccgaga ttttgacgat gtctattttt
    18661 ccaacgataa cgggctggaa gagacgcgtt atgtttttct gggaggcaac caattagagg
    18721 tacgctttcc tgagcatcca catcctctgt ttgtggtagc agagagcggc ttcggcaccg
    18781 gattaaactt cctgacgcta tggcaggcat ttgatcagtt tcgcgaagcg catccgcaag
    18841 cgcaattaca acgcttacat ttcattagtt ttgagaaatt tcccctcacc cgtgcggatt
    18901 tagccttagc gcatcaacac tggccggaac tggctccgtg ggcagaacaa cttcaggcgc
    18961 agtggccaat gcccttgccc ggttgccatc gtttattgct cgatgaaggc cgcgtgacgc
    19021 tggatttatg gtttggcgat attaacgaac tgaccagcca actggacgat tcgctaaatc
    19081 aaaaagtaga tgcctggttt ctggacggct ttgcgccagc gaaaaacccg gatatgtgga
    19141 cgcaaaatct gtttaacgcc atggcaaggt tggcgcgtcc gggcggcacg ctggcgacat
    19201 ttacgtctgc cggttttgtc cgccgcggtt tgcaggacgc cggattcacg atgcaaaaac
    19261 gtaagggctt tgggcgcaaa cgggaaatgc tttgcggggt gatggaacag acattaccgc
    19321 tcccctgctc cgcgccgtgg tttaaccgca cgggcagcag caaacgggaa gcggcgatta
    19381 tcggcggtgg tattgcctgc gcgttgttgt cgctggcgct attacggcgc ggctggcagg
    19441 taacgcttta ttgcgcggat gaggcccccg cactgggtgc ttccggcaat cgccaggggg
    19501 cgctgtatcc gttattaagc aaacacgatg aggcgctaaa ccgctttttc tctaatgcgt
    19561 ttacttttgc tcgtcggttt tacgaccaat tacccgttaa atttgatcat gactggtgcg
    19621 gcgtcacgca gttaggctgg gatgagaaaa gccagcataa aatcgcacag atgttgtcaa
    19681 tggatttacc cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa caaattacgg
    19741 gcgttgcgac aaattgcagc ggcattactt atccgcaagg tggttggctg tgcccagcag
    19801 aactgacccg taatgtgctg gaactggcgc aacagcaggg tttgcagatt tattatcaat
    19861 atcagttaca gaatttatcc cgtaaggatg actgttggtt gttgaatttt gcaggagatc
    19921 agcaagcaac acacagcgta gtggtactgg cgaacgggca tcaaatcagc cgattcagcc
    19981 aaacgtcgac tctcccggtg tattcggttg ccgggcaggt cagccatatt ccgacaacgc
    20041 cggaattggc agagctgaag caggtgctgt gctatgacgg ttatctcacg ccacaaaatc
    20101 cggcgaatca acatcattgt attggtgcca gttatcatcg cggcagcgaa gatacggcgt
    20161 acagtgagga cgatcagcag cagaatcgcc agcggttgat tgattgtttc ccgcaggcac
    20221 agtgggcaaa agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg cgttgtgcca
    20281 cccgcgatca tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca ctcgtggaat
    20341 atgcgtcgtt ggcggagcag aaagatgagg cggtaagcgc gccggttttt gacgatctct
    20401 ttatgtttgc ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt gccgagattc
    20461 tggcggcgca gatgagcgac gaaccgattc cgatggatgc cagtacgctg gcggcgttaa
    20521 acccgaatcg gttatgggtg cggaaattgt tgaagggtaa agcggttaag gcggggtaat
    20581 ctgctctcgc agtgtttgcc ggatgcggcg tgaacgcctt atccgaccta cgtgtgacgt
    20641 gtaggcctga taagacgcga taagcgtcgc atcaggcatg gtgctccaga cgccgcaact
    20701 tactgctgtg acgcctgctg aaacaggttt tcccacatat cggtcaccag cgcctggtca
    20761 cgcggcgaca attcaccggc accaatggct ttttccagac tctggctaac ggtcgtatgc
    20821 accgcctgag cggagtggtc atcaccactt tccagttctg cgatggctaa tgtcaggtgg
    20881 ccacgcaaat acccactggc aaacaactca tcatcactgg catggtcaat catgccgtcg
    20941 attaatgcca gaatgcgtga ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg
    21001 tggcaccggc ttcagttgag cgcttccggc cacgggaact gttccgccgt aagttccggc
    21061 gtgtgataat aattttgtag tgctttaatg aagcgtgccg gacgctcggg aatgccattt
    21121 gtcagataat ccatcacctg cgcatgtacc cgccgttgaa acaccacacg atccggttcg
    21181 aagtcgcctt ccagattgtc gcaactgacg ttaaacggat atcccgccgc cacacagaac
    21241 aaccagtcga gtgcctgagg cttcacttca acatcttcaa actggctttg cgtttgggca
    21301 tcacgcccat ccgggcaata ccagtagccg aaatcaacca gctcacggcg cgcttttccg
    21361 gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat aaaagccgtg ggcaaagaca
    21421 attcggttat acggtacttc cgcatcagca ggaagataga tcggttcgtc gtcgccttta
    21481 atcagacggg tattaaattc atcggcaaag cagctattaa aaatttcaat caactgctca
    21541 tagtggtgtg tactgttcat tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc
    21601 tatcgtaaag taatttggca ctcatcaccg ccgagacgat aacgatcatc gggcggatca
    21661 gtttttgtcc tttgctcagc accagtcttg aacccatgcg cgcgccgagg aactgcccca
    21721 ccagcatgac aaagcccgtc gcccaaatca ctttgccgcc gagaataaac agcagcagac
    21781 cgccaatgtt tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga
    21841 atccgcacag cgtaacgaag gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc
    21901 cgtcataaaa accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc
    21961 gctggcgatc ttcttcaccc agctttggca tcagcaagaa atagaggccg atacaaatta
    22021 ccagaatggg caaaatctgc cgtaagacat cagcctgaac gtattgcacc agcagtgcgc
    22081 cgctcattga gccgacaaag gtcatggcga tattgagttt ctgatcgctt aaactaacca
    22141 ctttgcggcg aataaagtag atagtggcgg aaatagagcc gccgcaggct tgcagtttat
    22201 tggttgccag cgcgttagcg ggagacattc ccgcagccat caatgccgga atggtgagta
    22261 acccaccgcc accggcaatc gagtcgataa atcccgccag catggcgaca aaaaagagaa
    22321 ctcccagcaa cagcggggaa accataaaca ggctattaaa cgtttccatt agatcacgtg
    22381 ctcatccagt agcgcctggc aggaaggcgg caacggaggc ggtgtcttct tctcaggctt
    22441 tgttgttcct ggttttggag gtgcaaacca gctttgcagt tctgccccgc aaccatcgcc
    22501 tggtggcggt aaaggttgat cttcacactc cagactatcg gcagggcaac gtaatcgtac
    22561 atgcatatgc gcgcgatgct ggaaccaagg tcgcactttg cgcaaccagt cgcgatcggt
    22621 gcccgcatca aggcaaagtt gttgtttaat cgccggatta acaaaaatgc gcgtgacgtc
    22681 tttgtcctgg gcggcgagtt tgatcaagct gaaaatttct ggcttccaca gcgtggagac
    22741 aacgtgttta ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga
    22801 ggtccagcga gttttcggca gttgcagaaa gatatcgaca tccagtccgg tctggtggct
    22861 ggcatgaccg ccgttgaaac gcccaccagc gggcatcccc atatcgccaa tcagcaccgt
    22921 acccatgccc agattgctca cctggctact cagacgctgg ataaacatca ccagatccgg
    22981 gtgaccgaaa tagcgacgct gatcggtacg catgacctga taatgttcgg actgtatcgg
    23041 caacgtgtca gcgccgacaa tacagccatt agaaaaactg cctatcgatt gtgcgctacc
    23101 cggcacaggt tgggttattt tttgccacgg cgtcgctgcc aagctagcgc tactggcaag
    23161 cagagccagc agcgcaatcg cggttttgtt cattttttac cagcgtggaa tatcagtctt
    23221 cacatcggca ttttgcgccc gctgccgtaa caggtgatcc attaaaacga tcgccagcat
    23281 cgcttcagcg atcggcactg cacggatccc gacacaggga tcgtgacggc ctttggtgat
    23341 catctcaact tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa tgctggaggt
    23401 cggtttcagc gccatatgag caatgatttg ctgcccgctg ctgataccgc cgagaatgcc
    23461 gcccgcatgg ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg
    23521 cagcgccact acgtcaaagc cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat
    23581 cagcgcatgg gcgatgtcgg catccaggcg gtcaaatacc ggctcgccaa gtccggcggg
    23641 gacgccactg gcaacaacgg tgactttcgc gccgatggag tcgccctctt ttttcagcgc
    23701 gcgcatcagt tcatctaacg cgtcgatttt gtccggatcc gggcagaaga acggattttg
    23761 ttcaaccagc gaccagtctt tgatttccag cggaatgtcg cccatctggg tcaagcagcc
    23821 gcggatttca ataccaaatt tctcggcgag atattttttg gcaatcgccc ctgccgccac
    23881 gcgcatggcg gtttcgcggg cggaagaacg tccaccgccg cgataatcgc gcagaccgta
    23941 tttttgttcg taggtgtaat cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta
    24001 atcctgagag cgctggtcag tgttttcgat caacaagcca atgctggtgc cagtagtaac
    24061 gccttcaaaa acaccggaga gaattttgac ctgatccggc tcgcggcgct gggtggtata
    24121 gcgcgatgtc ccagggcgac gacggtcgag gtcatgttgc aggtccgctt ccgtcagcag
    24181 aatgcctggc ggaacaccat cgacgatgca gccgagcgcc agcccatgcg attcgccgaa
    24241 ggtggttacg cgaaagagtt gtccaattgt gtttccagcc atcacggctc cgttattgtt
    24301 gtgtttgcgt gtttacttaa tctttataaa tcgcgaaata ttcacgggcc gccagcagct
    24361 gctctttggt gagcataaac acaccatcgc cgccgttatc aaactccagc caggtgaacg
    24421 gaacatccgg atattgttcc ataagatgta ccatgctgtt gccgacttca caaatcaaca
    24481 cgccatcatc agcaaggtaa tctgccgcgt ttccaagaat gcgacgcgtc agtttaaggc
    24541 cgtcggtgcc agatgccagg cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg
    24601 acatatcttc cgcgtcgaca tacggcgggt tagtgacaat cagatcgtac tgcactttcg
    24661 gcaagtcgcg gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt
    24721 cgatgttctg ttcagcaacc gccagcgcgt cgggagagat atccaccgcg tcgacttctg
    24781 catccgggaa ggcataagca caagcaatgg cgatgcagcc gctaccggta cacatatcta
    24841 aaatatgctg cggttgtttg ctgatgagtc cggcaaattt attgttgatc agttcaccaa
    24901 tcggcgagcg cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg
    24961 ctttgttggt caggtaagcc accggaatgc gctcgttgac gcggcggatc acgcgttcaa
    25021 caatacgatg tttttcgctg gaggtcagac gcgcggtgcg catatcttcc gggatatcca
    25081 gcggcaggta gagcgatggt aacaccagct gtacggcttc atcccacggg ttatcggtgc
    25141 cgtgaccgta ccagatattt gccgcgctga agcggctcac tgaccagcgc aacatgtcct
    25201 gaatggtttg cagctcattt actgcttcat caacgaaaat tttatccacg tattcctcca
    25261 gggcatgatc gcaataattt cggcggctag tttgccatga agatgacgat aaatcagcat
    25321 tcacgcgcgg tgagtgagga aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt
    25381 aaactgtagg aaaattagaa atagagacag ataaatgaaa aagaaaacaa cacttagcga
    25441 ggaggaccag gctctgtttc gccagttgat ggcggggact cgcaagatta agcaggacac
    25501 gattgtccac cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca
    25561 ggagcaggct gatgccagcc attatttctc cgatgagttt cagccgttat taaataccga
    25621 aggtccggtg aaatatgttc gcccggatgt cagccatttc gaggcgaaga aactgcgccg
    25681 cggcgattat tcaccggaat tgtttttgga tttacacggt ctgacgcagc tgcaggccaa
    25741 gcaggaactg ggcgcgttga ttgccgcctg tcgccacgaa catgtgtttt gtgcctgcgt
    25801 gatgcatggg cacgggaagc atattttgaa gcaacaaaca ccgctgtggc tggcacaaca
    25861 tccgcatgtg atggcatttc accaggcacc gaaagagtac ggcggtgatg cggctttgtt
    25921 ggtgttgatt gaagtggaag tgtggttgcc gccggagttg ccctgaggag ttgagcgtgt
    25981 tgtgccagga gcgcggaatg gcaggggcga atgaccctgc caggtgagaa aaggtgcgct
    26041 aatacatatt cagtatgtaa gtgaatcggc aggtagaatc tatgggctgt cgagatgtcg
    26101 tgtccacgcg tagcttcata tcatgcagtt gcctggacgt aatcggttgc ggctcaggcg
    26161 tcaccgtcac tttggcatta tcacagccgg aagtcgagaa ggtcatctgc tgtgcgataa
    26221 aacggcagta gacaccacgc tcatcaaaat tattcccgtt acacagtttg ccattttcca
    26281 tcaggaaagt gggcagtgtt tgctcaataa caccacctgt tgactgcaac ctgacgactt
    26341 tgttatgtac aacataattt tcattccagg aaacatcata cggtgccagg ggcaccgccg
    26401 gacatgaaga actcaccttc gttgggccaa tatagtcagg aaaatcatca tcatacgttt
    26461 tagcgaaggg atctctactc ttaacaaacg acgacacaat ccacggacaa ccatgtaaat
    26521 tcccatcgta ataaacaaac atctttaatt cattacatgt ccatcccgtt ttggtctggc
    26581 tatcatactg ctctaatcca ggacaggctg acatggatac cctgctgtcc gaaattttcg
    26641 acatagatgg ttgagtagaa ctatcgccag tccacgaaac taaatccggc tgtgaattgg
    26701 gcatgaatgt agccttaaat atagcgtaat taactgaatc tggcgtcgct ataatcagat
    26761 gaaactcaaa ct
//
LOCUS       DAJTMK010000040        26755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTMK010000040 DAJTMK010000000
VERSION     DAJTMK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26755
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.041"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            515..1414
                     /locus_tag="OO135_001443"
     CDS             515..1414
                     /locus_tag="OO135_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5397351.1"
                     /translation="MDKIHAIQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKEVLSNLNELDGLFQQDATSISGILRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            1463..2476
                     /gene="yhjD"
                     /locus_tag="OO135_001444"
     CDS             1463..2476
                     /gene="yhjD"
                     /locus_tag="OO135_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5397352.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            2887..4209
                     /locus_tag="OO135_001445"
     CDS             2887..4209
                     /locus_tag="OO135_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5397353.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4443..6503)
                     /locus_tag="OO135_001446"
     CDS             complement(4443..6503)
                     /locus_tag="OO135_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5397354.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLIKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(6573..7340)
                     /gene="pdeH"
                     /locus_tag="OO135_001447"
     CDS             complement(6573..7340)
                     /gene="pdeH"
                     /locus_tag="OO135_001447"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5397355.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            7572..8501
                     /gene="kdgK"
                     /locus_tag="OO135_001448"
     CDS             7572..8501
                     /gene="kdgK"
                     /locus_tag="OO135_001448"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5397356.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(8597..10093)
                     /locus_tag="OO135_001449"
     CDS             complement(8597..10093)
                     /locus_tag="OO135_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5397357.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(10314..11600)
                     /gene="dctA"
                     /locus_tag="OO135_001450"
     CDS             complement(10314..11600)
                     /gene="dctA"
                     /locus_tag="OO135_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5397358.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(11783..13771)
                     /gene="hmsP"
                     /locus_tag="OO135_001451"
     CDS             complement(11783..13771)
                     /gene="hmsP"
                     /locus_tag="OO135_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5397359.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIACELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLLLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(13853..17349)
                     /gene="bcsC"
                     /locus_tag="OO135_001452"
                     /pseudo
     CDS             complement(13853..17349)
                     /gene="bcsC"
                     /locus_tag="OO135_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            complement(17331..18437)
                     /gene="bcsZ"
                     /locus_tag="OO135_001453"
     CDS             complement(17331..18437)
                     /gene="bcsZ"
                     /locus_tag="OO135_001453"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5397360.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(18444..20783)
                     /gene="bcsB"
                     /locus_tag="OO135_001454"
     CDS             complement(18444..20783)
                     /gene="bcsB"
                     /locus_tag="OO135_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5397361.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVVAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(20794..23412)
                     /gene="bcsA"
                     /locus_tag="OO135_001455"
     CDS             complement(20794..23412)
                     /gene="bcsA"
                     /locus_tag="OO135_001455"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR5397362.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYFIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(23409..24161)
                     /gene="bcsQ"
                     /locus_tag="OO135_001456"
     CDS             complement(23409..24161)
                     /gene="bcsQ"
                     /locus_tag="OO135_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5397363.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYLWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(24173..24361)
                     /locus_tag="OO135_001457"
     CDS             complement(24173..24361)
                     /locus_tag="OO135_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5397364.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            24634..26118
                     /gene="bcsE"
                     /locus_tag="OO135_001458"
     CDS             24634..26118
                     /gene="bcsE"
                     /locus_tag="OO135_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5397365.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAEHDGRHWRRIPEPMRLLDDAVERSS"
     gene            26115..26306
                     /gene="bcsF"
                     /locus_tag="OO135_001459"
     CDS             26115..26306
                     /gene="bcsF"
                     /locus_tag="OO135_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5397366.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            26303..26662
                     /gene="bcsG"
                     /locus_tag="OO135_001460"
                     /pseudo
     CDS             26303..26662
                     /gene="bcsG"
                     /locus_tag="OO135_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
BASE COUNT         6235 a         7392 c         6790 g         6338 t
ORIGIN      
        1 aatctccacc tgaatatgtt aaaaagactt aagtagtgga agggtattac ccgcgagaaa
       61 aaataagaat tcgccatttg gcggtggcca ttctacagag atgacgcgta gaaaatagtt
      121 accgatataa atagttacag ctaaacgcct gaaattacat gtcgagggca ctatttaaaa
      181 caattttgag gatttcctta tattggtggt tagtacgcat gcaattaaaa atgaaattcc
      241 gcgactacaa gccaaaataa caaatggcaa tgagacaaaa ataagcacaa atagccaata
      301 cgtcctctat tcactttaaa gggaatcact gaaaaatacg ctctgtttaa ggggattcac
      361 ctttctcata aagctattcc gcccttttcc tgctgagaaa tcgccacatt cgtcatgaca
      421 acattgtgaa acccggcatt agatgttaga aaaaacaata aacaatgcga tatgcgcgtt
      481 actgtttcgc tgtgaataac aatcggtcaa agaaatggat aaaattcacg caatacagtt
      541 gttcatcaaa gtcgcggagc tggaaagttt ttcccgcgca gcggatttct ttgctttgcc
      601 aaagggaagt gtttcgcgcc agatacaggc actggaacat caacttggca cccagcttct
      661 ccagcgcacc acgcgacggg tcaaactcac gccagaaggc atgacctatt atcaacgggc
      721 aaaagaagtg ttgagtaatc tcaacgaact ggacggtctg tttcaacagg atgccaccag
      781 tatcagcggt atattacgca tcgacatccc gccaggaatc gcgaaaaacc tgttactgcc
      841 gcgcctgtcg gaatttctct atctgcatcc gggaattgag ctggaactga gtagccatga
      901 ccgtccggta gatattcttc atgatggttt tgattgcgtg atacgcactg gcgcgttacc
      961 ggaagatggc gttatcgccc gtcccctcgg caaactgacc atggtcaact gtgccagtcc
     1021 gcactatctg acgcgctttg gttatccgca aagccccgat gatctgactt cacacgcaat
     1081 agtgcgttat acaccgcacc tgggtgtaca tccgttaggt tttgaggttg ccagcgttaa
     1141 tggcgtccag tggtttaagt ctggcggcat gttgacggta aatagtagcg aaaactatct
     1201 cgccgccggt cttgccggtc tggggattat tcagatcccg cgcattgccg tgcgcgaagc
     1261 cctgcgtgcc gggcggctta ttgaagtatt acctggctac cgtgccgagc cgctctccct
     1321 ttcgctggtt tacccgcagc gtcgggagct ttcccggcgt gtaaacctgt ttatgcagtg
     1381 gctggctggc gtaatgaaag agcacctgga ctgaccgact atacttttta agaatgacca
     1441 cgacagaagg acaaaagagc ggatgacgca ggaaaacgag atcaaacgtc ccacccagga
     1501 tctggagcac gagccgatta agcagttgga taatagcgag aaaggcggca aagttagcca
     1561 ggcgctggaa accgtcacca ccactgccga aaaagtccaa cgccaaccgg ttattgcgca
     1621 cctgattcgc gcgacagaac gctttaacga tcggctgggt aaccagtttg gtgcggctat
     1681 cacctatttc tcatttttgt cgatgatccc gattttgatg gtgtcgtttg ccgcaggggg
     1741 ctttgtgctg gcctcccatc cgatgttgct acaggatatc ttcgacaaaa ttctgcaaaa
     1801 catcagcgat ccgacactag ccgccacgtt gaaaaacacc atcaacaccg ccgttcagca
     1861 gcgtacgact gtagggcttg tcggcctggc ggtggcactt tattccggca tcaactggat
     1921 gggtaacctg cgtgaagcga ttcgtgccca gtcgcgcgat gtctgggaac gctcgccgca
     1981 agatcaggag aagttctggg taaaatatct gcgtgatttt atttcactga ttggtttgtt
     2041 gattgcgctg attgtgacgc tttcgatcac ctcggttgcc ggttcggcgc agcaaatgat
     2101 tattagcgcc ctgcacctga acagcattga gtggctgaaa ccgacgtggc gattgattgg
     2161 cctggcgatt tccatcttcg ccaactatct gcttttcttc tggatcttct ggcggttgcc
     2221 gcgccaccgt ccgcgcaaaa aagccctgat tcgcggaaca tttctggctg ctattggttt
     2281 tgaagtgatt aaaatcgtga tgacctacac cctgccatcg ctgatgaaat ccccctctgg
     2341 cgcagcattt ggttccgtgc tggggctgat ggcatttttc tacttcttcg cccgtttgac
     2401 gctattttgc gcggcgtgga ttgccaccgc cgaatataaa gacgacccgc gaatgccagg
     2461 gaaaacgcag ccttaaaata ggccggatgc ggcgtaaacg ccttatccgg catacgattt
     2521 tattcggcct ggctccccgt aggccggata agatgcgcca gcatcgcatc cggctataat
     2581 gcgcacataa cctcttgaaa ctcgtcccca gagtctcttc agccatctat ttaggagcaa
     2641 acaatttcat tctaactcat aaccccagca tataaatcca attggtaact tttatttaac
     2701 ctgaaaccag ttttatccac tatttataaa attatgtgaa gcatttcata gaagaaaaat
     2761 cactggccta aacattatcc cctttttgcc tggtttttga ccatttccgc gatttgttac
     2821 acattgaaat atcacttttg ctgtgcgtaa tatggctatt cgttagccaa aaaataagaa
     2881 aagattatgc aagcaacagc cacaacactc gaccacgagc aagaatacac gccgatcaac
     2941 tcgcgtaata aagtccttgt cgcctctctc attggcacag ccattgagtt cttcgacttt
     3001 tacatttacg ccactgcggc cgttattgtg tttccgcata tcttcttccc gcagggcgat
     3061 cctacggcag caacgctaca gtcgctcgcc accttcgcca tcgccttcgt cgcgcgcccc
     3121 attggctctg ccgtttttgg tcattttggc gatcgcgttg ggcgtaaagc gacgctggtc
     3181 gcctcgttgc taacgatggg gatttcgacc gtggtgattg gtctgctgcc aggctatgcc
     3241 acgattggta ttttcgcccc gctgctgctg gcgctggctc gatttggtca gggtctgggc
     3301 ttaggtggtg aatggggcgg cgcggcgctg ctggcgactg aaaacgcccc accgcgcaaa
     3361 cgtgcattgt acggctcctt cccgcagctg ggcgcaccga ttggcttctt ctttgccaac
     3421 ggcactttct tgctgctttc ctggctactg accgacgagc agtttatgag ctggggctgg
     3481 cgtgtgccat ttatcttctc ggcggtgctg gtcattatcg gcctgtatgt tcgcgtgtcg
     3541 ctgcatgagt cgccggtgtt tgagaaagtc gctaaagcga aaaaacaggt gaagatcccg
     3601 ctgggtacgc tgctgaccaa acatgttcgc gtaaccgtac tgggtacgtt cattatgctg
     3661 gcaacctata cgctgtttta catcatgacg gtctactcta tgacctttag taccgctgcc
     3721 gcgccagttg ggcttggcct gccgcgtaac gaagtgttgt ggatgttgat gatggcagtt
     3781 attggttttg gcgtgatggt gccagtcgct ggattactgg ctgatgcctt tggtcgccgt
     3841 aaaagcatgg taatcatcac cacgctgatc atcctgttcg cgctattcgc ctttaaccct
     3901 ttgctcggtt ctggcaaccc gattctggtt tttgccttcc tgctgctggg gttaagtctg
     3961 atggggctga ccttcggtcc gatgggcgcg ctgttaccag agctgttccc gacagaagtg
     4021 cgttacaccg gagcatcgtt ctcttacaac gtatcgtcga ttctcggggc ttccgttgcg
     4081 ccgtatatcg cagcctggtt gcagactaac tacgggttag gtgcggtggg gttatatctg
     4141 gcggcgatgg ctggcttgac gttaatcgcc ctgctgctga cccatgagac gcgacatcag
     4201 tcgttgtaag tatcgtgtgg gcagccaccc tcaccccagc cctctccctg aaagggagag
     4261 ggagaaaagc ggagcgagat gttatatcgc gaacataaca gccctccccc agaaagtgaa
     4321 cggaaaagcg gtactcgttg tttcaacacg cacaaaacag tcccctcgcc cctttgggga
     4381 gaggattagg gtgaggggaa cacgctgctc ccgaactcac cagcaaacac aatccaacct
     4441 catcacttct tcatctgcga caatatcgtc cgacactgat tagcctcccc ttcggaaggt
     4501 gagatcagtg ccaataacgc cgccgccggt gttaccagcg ttgccagtgc cgccgcaacg
     4561 gcaccacggg caatcagcgg tccggctttc accccagcct gcggattttt aaacgtccca
     4621 cgcacataca gcggcgaacg cagtgtgata atccgaatcc ctttactctc cggatcaata
     4681 gtcaaatcca gctgttccga agcaaaactt gccgtgccgg taacattaat caacgcgttc
     4741 tcagtatcga aagcaaaaat ctgcgggcgc gctacgccgt tggcaatatt cagattcgcc
     4801 gccgcgcagt tcacccgcac ctcatcgtca ccaaatatcg caccgacaat gtagttgccg
     4861 acattcagcc caacaatctc catcaggttg cggctcacca gcccgtcatt catcaacagt
     4921 ttcaggttgc cgttactgtt gcctaaaagc gccgccaccg agttaccgct accgcgtagt
     4981 tccgcgtcac cgttcatttc ccccagcgtc ttctgcatca gttccacatc gggcatcagt
     5041 tctttcagtt tcagtcgacg agcctgaata tctgcccgcc cctgcatcgg ctttttatcg
     5101 ccttccagat ggatattcgc cgcaatgctg ccgcccgcca tgccaaattt cagcggttgc
     5161 aggcgcaggt cagcattttt gaggatgata tgagtagaaa gatcgctaat cggcaggcta
     5221 ctgccatgct cgatacgtcg gcctttgaag cgaacatcgg catccataac gtcccattta
     5281 tcggtttcga agcggtcata aggcagcact ttgcccgcag gctgaacgct tttttcgccc
     5341 ttcttctgtt cagaccgttt cgacttttct gcccctttcc ccgaatcaac gccaatcaac
     5401 ggtcccaggt ccgccagccg caattgccgc gactcgacat caccttccag ttttggtcgt
     5461 ggctttccgg tggtgtagac cagagaaccg tggatatcgc tatcgccaat tcgcccatta
     5521 aaaccgcgat aatcaaagac cgacgatttt tcagtgtcga ttttcgctac cagccgacca
     5581 tccgtttcaa acggcggggt atcgggcagc agaacgcctg tcagttcata gagatcaccc
     5641 agtgaatcgc cagaaaattt aagccgtaaa tcgacaccgc ccatcttcat tgggtcattc
     5701 acgacgccat caaaagcaac acgggtatta cctgaacgga aatcagcctg taccggaaac
     5761 ggcgtccctt cgccacgcag cgccagcata ccgcctattt ttcccgtacc cgtgagctgt
     5821 tcaccgttat aacgtccctg cgccttcagg ccaaaaacgt aatcgcccac cttttcttta
     5881 tccgctttac ctttcgatcc agtaacttcg ctgaacggca gcggcttgcc taacggatca
     5941 acaaaaattt ccagatccgc tttgcttact ttgtcatcaa tggcgatccg cccttgatcg
     6001 aaaagaatat tatccagccg aaacgaccat gccgacggct ttgcattcgc gtctttgtta
     6061 tcatcgttgg cgagattaaa cgtccagtta ttgttctttt cggagaggcg aatcaggcgc
     6121 gcgtcgggct tttcgagctt gatccacggc agccagaccg ttttgatcag cagcgccagc
     6181 ggtgccagcg ttgcctctac tcgcggcaaa tgcaccatcg tgacttcggg aatatccggt
     6241 ggattgccaa gaatgatgtc ttctgcatgt acatgaggcc acggcaccca gctgcgccag
     6301 ccagtttctt gtttttgccg ctcccacacc acgcccagat cgccacggat agcgaacgga
     6361 cgattcaact ccgcagagac tttctggttg atggtcggtt tgagtcgatt ccagtcaaaa
     6421 gttgcaatca aaatgatcgc cacgacaatc aacaacaaga aagcccctga aatcgcagcg
     6481 gttattttgc ctgccttgct catcgttcgc ttcctcatat tcttcctgtg ccagtcctaa
     6541 agatagtcca gccaggcgga aaatgaggca gcttatagcg ccagaaccgc cgtattcagc
     6601 gtttctatcg gtgccgggcg tgaaagaaac cagccttgtg cggcgaatgc gggcgagttc
     6661 tgtacatcac gccactcttc cggcgtttct acgccctcaa caatcacccc gcgacaatag
     6721 cgattcatta gatgtaaaag ctgagaaaag agtgtgcgtc cttccggcga ctgacgcagc
     6781 atcacaaaca gttctcgcgc gattttgatg tagtcataac gcacttcact tagtgcagag
     6841 aaatttgcca tcccggtacc aaaatcaccc agccacagcg ggccaaattc acacatcgag
     6901 gcaaaggttg aatctttcgg cagacggata tgctccacca gttcgaaacg cagccaggga
     6961 agacgctcaa tttggcgcag gatttttggt tgctgacgca gggcgatgag cgtagggcca
     7021 tcaatattga ccgatgccag caggccgtgc tctataaaga agtcggcttt ttgcgccagc
     7081 aaatcaatct gctctttcac aacctccatc cgatggctga cggtgatttc agtaaaatag
     7141 cgatccggcg gcaggcgttg cgaagggttc aagggatgcg tgaccaccgt taatagctcc
     7201 acggccatta accgcccgca tgtttgatag atcggctgcc aggtgtaagc acgctcacac
     7261 tgcaaccaaa aacgccgttc ctgcaagctc tcgatgcttg cttcagggtt gcttattcgc
     7321 tggataacct gccttatcat caaagatgtc ctgtgtttaa aagtgatgct gcccggactc
     7381 gacaaagatt atcggcgcgt aagggcagaa ctttaccgcc tggccggggt aaatttcctc
     7441 actccacaac ggaatctttc aggaatacgt tctggctcaa aaaaataatg gaacactgtt
     7501 ttaatattgt tgaccagcaa accaccacag cgcaaactaa cgctaatttt ttacagttca
     7561 ggttcacgac tatgtccaaa aagattgccg tgattggcga atgcatgatt gagctttccg
     7621 agaaaggcgc ggacgttaag cgcggtttcg gcggcgatac cctgaacact tccgtctata
     7681 tcgcccgtca ggtcgatcct gcggcattaa ccgttcatta cgtaacggcg ctgggaacgg
     7741 acagttttag ccagcagatg ctggacgcct ggcacggcga gaacgttgat atttccctga
     7801 cccaacggat ggaaaaccgt ctgccgggcc tttactacat tgaaaccgac agcaccggcg
     7861 agcgtacgtt ctactactgg cggaacgaag ccgccgccaa attctggctg gagagtgagc
     7921 agtctgcggc gatttgcgaa gagctggcga atttcgatta tctctacctg agcgggatta
     7981 gcctggcgat cttaagcccg accagccgcg aaaagctgct ttccctgctg cgcgaatgcc
     8041 gcgccaacgg cggaaaagtg attttcgaca ataactatcg tccgcgcctg tgggccagca
     8101 aagaagagac acagcaggtg taccaacaaa tgctggaatg cacggatatc gccttcctga
     8161 cgctggacga tgaagacgcg ctgtggggtc aacagccggt ggaagacgtc attgcgcgca
     8221 cccataacgc gggcgtgaaa gaagtggtgg tgaaacgcgg ggcggattct tgcctggtgt
     8281 ccattgctgg cgaagggtta gtggatgttc cggcggtgaa actgccgaaa gaaaaagtga
     8341 tcgataccac cgcagctggc gactctttca gcgccggtta tctggcggta cgtttgacgg
     8401 gcggcagtgc ggaaaacgcg gcgaaacgcg ggcacctgac cgcaagtacc gttattcagt
     8461 atcgcggcgc gattatcccg cgtgaggcga tgccagcgta aacgcagcat atgcaaaagg
     8521 ctggataaag tgttacccct ctatccagct catccataaa tacctgatgg ttgtgctaac
     8581 tcatcaccat caggcattac tgtgcaggtg gaatatccgt cacttcagga tgtacgtcat
     8641 ccgtggcgac agaggtggcg gcagcagcag tcgatggggc catgatttga tcccaggccg
     8701 cctgcaacgc tttcatgtta aattccggct cgcctttcgg ctgcagcagt attaacgcca
     8761 tatcattcga caactgctga cgcagatcct gatttaacat ctccaccgtc aggctgttaa
     8821 ggaaatcctg ccgcagtttc tgatactgct ccggcgcgat atcgacgacc tgattttgca
     8881 acgaacgcat ccgctgaccc atcagaatat cggtatcagc ccgcgcatag gcggcaaaca
     8941 gtttctgcag ctccagtttc ttttgcgcca ctaacgcatt gaactcttct tccggcagac
     9001 ctttatcgcg aaccttcgcc agttcacgcg ccaccagatt aaggttgctg ttcagcttgt
     9061 cgtttggtga ttcgatgttg atggcacact gcgcacgcag atacagcaca cggcagtcaa
     9121 atccaagacc gatgtctttg ctgttactgg cgcttaacgc ttgctgaaca tgccagaaca
     9181 gtgcctcgcg ggccaggtcc gcacgccagt agcgcagcag tgcggctgat tcacgaatcg
     9241 gctgccacgg cgtatcccac atgatagata accggtcctg acgcaccgcg tcagtcataa
     9301 tactcaccgc ttccgcacgc agcggagaaa gcgtcggcac cggagctggc gtttcacgtt
     9361 tgcctttcag ttcgccaaac gttttgttga tttggtcgac aaccgagcgc gcatccacgt
     9421 ttcccaccac cagtagcgtc attgcatccg gggtgtacca tttctgatag aaatctttaa
     9481 ttttttccgc ttcaacgggt tgtttcagcg gatcggcagg atcgtgacct aacaaggttg
     9541 accctttcag acgatagcgc caccagccct ctttagtatc ggcaggccag gttgccacca
     9601 tgtcctgact ttgcagcgcg tggttgatgg tttctggtgt gatggtcaat ttgccagtgg
     9661 catttgccag ataagagagc gcttctttca gcaaatcgtt acggttattg ggcaaactca
     9721 gattaaacag cgtggtgtca taagagacaa ttaccggcgg catcgggcgt ttagggtcga
     9781 tcccctgctg ccacaatgaa cgcgcctgtg ctgcgtcaag gccaccgctt tgcgttagcg
     9841 caatacgagg gatggcgtga ctgtaaccgc tctgttgtgt actttcggcg agcgaaccgg
     9901 tattaaccag caggcgaatt tcaacacgat cgctgggacg ctggggggtg gtcagcactt
     9961 gccactgtaa accgttggaa agcgtcccct gttgccatgc tggatcaggc tggagcgcat
    10021 ctgcctgcac atagccagca gtggccatca tcagcaaacc gcccgctaaa agtcgaattt
    10081 ttgtgccctg catgtgaacc cctgatcaac tatcctggta ataaaaaaac tgcccgttgt
    10141 cagtcagtct aaatgacgtt aaaaacactt cgtgttagac cgcaagaaca tgaaaatgtc
    10201 acggaagaag tgaaataaac ctgaactcac ccaggtggta agttcaggca caggggtcaa
    10261 ttatgcgcaa acacccgcac tcggggaagg gagtgcgggc ataagtggtg agattaagag
    10321 gataattcgt gcgttttgcc atccggcgca cgattattca gcacatcgtc cagttttttg
    10381 tggtccagtt ctttcaccca cttagcaacg acaatggtcg ctacgccgtt accgaccagg
    10441 ttagtcagcg cacgagcttc tgacataaag cggtcgatac cgaggatcag cgccagaccc
    10501 gctaccggca aatggcccac cgcagagagc gtcgccgcca gcacgataaa gccactaccc
    10561 gttacccctg ccgccccttt agaagaaagc agcaacacga ttaacagcgt gatttggtgg
    10621 acgatatcca tctgactgtt agtggcctgg gcgataaaca ccgccgccat tgtcaggtat
    10681 atcgatgtgc catcaaggtt aaacgagtag cctgtcggga tgaccagccc caccaccgat
    10741 ttgcggcagc cgagtttctc catcttgtcg agcatacgcg gcagcgccga ctcggaagat
    10801 gaagtcccca gtacaatcag cagttcttca cggatgtagc ggataaattt gaagatactg
    10861 aaaccagtcg ctttagcgat tgaacccaat accagcacca caaacaggat acaggtaata
    10921 tagaaacaga taatcagctg ccccagttgc accagtgtgc cgacgccgta tttaccgatg
    10981 gtaaacgcca ttgccccgaa cgcaccaata ggtgccagac gcatgatcat attgatgatg
    11041 ccgaagatga cctgcgagaa actttcgatc acgttaaaaa taagttggcc tttgctgccc
    11101 agacggtgga gcgcaaaacc aaacagtacg gcaaacagca gcacctgcag aatgttaccg
    11161 ctggcaaatg cgccaatgac gctcgccgga atgacatcca taatgaaggc gacaatgccc
    11221 tggtctttcg cctgatcggc gtaaaccgct accgctttcg catcaagcgt tgccggatcg
    11281 acgttcattc cggcaccagg ctgcacgacg ttaacgatga taagaccaat aatcagcgcg
    11341 atggtactga caatttcaaa gtaaagcagt gcgactgcgc cggtacgacc gaccgccttc
    11401 atgctttcca tgcccgcaat gcccgttacg acggtacaaa agatgacagg agcgatgatc
    11461 atcttaatga gcttaacgaa gccgtcgcca agcggtttca tttgctcgcc tatttcagga
    11521 tagaaatggc caaggagaat accaatggct atcgctgtca ggacctgaaa gtaaaggctt
    11581 ttaaacagag aggttttcat agggtgtcct ttagtaaaac tacaggtctt gtaaggttat
    11641 gaggtacctg cggccttaaa ataacaccca gacaacatca cagaaatgta cctggatcat
    11701 aattgaaaca aaaaggttaa aaagtttgag ctggctcgca caaaccagca ctttttaaag
    11761 ttttgtaatc agtttggggt agctactttt cttccaggta actctcttcg aagatttcaa
    11821 tagggagtgg gcgagcaaaa aggaagccct gggcaatacc aacgcccgct tttgccagcc
    11881 agtcgcgttg tgcttcagtc tccacgcctt cggcaatcat ttgtaagttc aggctctgcg
    11941 ccagcatgat aattgcagca atcatgctgc tatcttccgg caagccttca acaaacattt
    12001 tgtcgatttt cagtacgtcg attggcaacg atttcatatg ctgcagctga cgcagccctg
    12061 cgtagcccat gccgaaatca tccagcgcca cccgaactcc ggcattgcgc agcggacgga
    12121 ggattgccac cgcagcatga gggtcgtcaa tacgtcggct ttctgtcact tccagaatca
    12181 gtgttcccgg ctgaatgcga tagcgggtta acagttccag catatccgcc accatatttg
    12241 ggtgcatcag ttgcagcgca gagaggttta ccgacaagag cagcataatg ccgcgctctt
    12301 gccaggctgc aagcaatcga caggactctt ccagcaccca gtgaccgacg gtaaccatca
    12361 gcccacagca ctcaatgcga tcgattaagc catccggcag gtcccaactg ccatccggtt
    12421 gctggatacg cagtaacact tccgcactga ccagtttacc gctggtcatc tcgacctgtg
    12481 gctgtaacca aatagcaaac tgatgatttt ccagtgcatt aaggatatcg ctctcttccg
    12541 tcaaccgctt ctgggcggct tccatctgct gcggatcaaa gaactgaatc tgattcttgc
    12601 ctttatggcg agcggtaaat gccgcagaaa tagcgcgact gtaaagctgt tcggcggtga
    12661 gatcgccgta gaacatcgcc acgccaatgc tacagtgcgg acggagttga atacgttcaa
    12721 tcggcaggcg ctcgctcatg atagtgagca cttgctgacc taaggtgatt gcgtgccacg
    12781 gttcctgtac accgttggca atgacagcaa agtcataacc gctaatctgc gcgaggatca
    12841 tacgtggcga tagtaccgat ttgagttttt ccaccagcgt cagcagcaga atttctcgtt
    12901 gcgcctcttt cagcacgccc gcagtatcac gcagggtttc acaggtgatg atcatcagcg
    12961 cggtggtttg tttacgcgcg acaacctgct ccagcatctc catcagcaag gctttgttcg
    13021 gcaaatccga caccgggaag cgcatcgcat tttcgttctg ttcttcataa tggcgctgca
    13081 gcaattgctg gttgaggttg taactgcgca ccaacatacc gatttcatcg tcctgatgca
    13141 gacgcggtaa tgccagttgg tgaccaacaa gctcctgggc tgggatggcg ttaagttcgc
    13201 aagcaatatt gcgtaacgga tgcaaaatca ggcggttaat gcaccagctg atggcgacgg
    13261 tcaggataag cgacaaaagt aagtaaatgg tcactaacgt tgagagggtg ctcatcacga
    13321 acttatacat acggaaggaa tccgcctgta gtaccagata ggcaattggc tgcgggtttg
    13381 ccggacgttc gagcgagtaa acgcccagcg agatttgaac cggtagctca aacaggcgag
    13441 taaccattac cggcaccggg cgctctggaa taaaactttt acgcagcgcc tggaactggt
    13501 taggcagcac tacatcggca cggctgacca cgcctgccgg tttaatgctg gcaagaatag
    13561 cttccgcttc gggaatatcg cctttcaaaa tagctgaaga taagggttcg cgtacagagc
    13621 gagcaatgct ttccagttgc gtagccgtgt tatagcgatt ctgctggacc agatggaaca
    13681 gcaaaacggt gcaaaaaata aaaacgaaca ccaggacaac ggctgccacc attgccatct
    13741 gcttgattgt taacgagcga cttacgcgca aattgtctcc ccccggaaat cccagattgc
    13801 cgcccgagta tacccgatcg cagcggcatt aagagaggcg ctatctgaaa acttaccagc
    13861 cggcgtaagg tatcagcggc tgcggcggta aatccatgtc accctgccat ccggcggcgg
    13921 aataacgtac gtagagcagg aaatggctgg gtgcgtaatc tttcgcctgc tggatatcaa
    13981 ttgccgtgcc aacaaaccag ttggaagtaa cacgtcgttc aagtaatgcc cgcgccgtgt
    14041 agccgaagcc ctgactgctg ccgccatcgt tggattgccg cgcagcttct tcctgccagt
    14101 cggtcgggat cagattcatc agcggataac gcggcatggt ttggtgcgtg aatgcgacca
    14161 cgagccagac gcccccagct cccacgacca gttttccgtg cgctcccgcc acatcaccgg
    14221 tatagcaaac gacaggtatt cctgcggact gtagtaaccg ccctgaccga gtgagtagcc
    14281 actcagatct ttgtcgtaat gccagatcat gttgttcagg ccgattgtga cgcggcgatt
    14341 gttctggtta atgaccttat agtaatagcc cgtcatccag cgcacgcgcc agttatcttc
    14401 gacattttta ccggttaact ggtcgccact aagcgatgcc cagacgccgt ttgcttcacc
    14461 tttatcgtag ctcagactta gccccacacc gtcggcacgt acgccacccc attttttccc
    14521 ggtattgctc ggggagtctt tttgcccacc aaaggccagc aaagaactgg agatgggccg
    14581 acggtgggcg ttaacggtgt aacccagcgg cccgatatca tcgctgtaac tgatgccgcc
    14641 gaccacatcc accacgttga agcccatcgg cgtggtaccg atatcccagc tccagacgtc
    14701 atttcgccag ccgaccgcca cgctggcacc ggagtccgac tggctgcggt tgccactaca
    14761 gtcctgtaat gtacaggtgc cccagttgtc atcccatttg ccatcggcat tagtggagaa
    14821 actgccgacg ttcatattga cgaaatcact gcgaaagaac atccgcccgt cagaatacgg
    14881 cgcatccacc tgcaacatgg tagtgtgcgc tttcagatcg gagtaaccac cggtgccgct
    14941 cgaaccccag taatcgtgct cgagggtgac gttaagatcc tgctggcgat agaggcccgc
    15001 cgcatcgctg cgcacgccgc gtttcagcca gtcatctttc tcgtcgttac gggtcagtcg
    15061 ggtgaatgtg tcgttatcct gcggacgcgt cgtagtcaca ccggatgcga ccatggcgtc
    15121 tttgtaggtt tccagcgcct gctttggatc gcccgcctgc gcttcaaact tcgcaccgtc
    15181 gcgcagcacc atcgcgcttt ccatcgacgg tggctgagat tttgcctgcg ggatcaactt
    15241 attaaacgtc tgctgcgccg ctgcggtatc tccaagctgc gcctgcgcct gcgcctgcgc
    15301 ctgcgccagc gccacgcgcc gctgtgtgtt cagcgaggcg ttatcggtag cgggcagctt
    15361 cgccagctgg ctacgtgccg ccgctgtgtc accggcagca atatccactt ccgtcagccc
    15421 cagaatggca tcggcgttag ttggccccag cgtcaggaca ttctgatatg cagcgcgggc
    15481 ggcggtgtaa tcacgtcgct gctgcgccca gtcagccagc gtgaggtcaa tacgcgtgga
    15541 aggtggttgc tgacgcagca tcgcttccgc ttccgcttca gcttctttgc cgctttctcg
    15601 caggcgatta gcggtttcca gcacctgatc gctttgcagt cgattaacca gctcctgaat
    15661 attgctgttc cactgcgcgc gcggcaggct gttgatatgc gccagcgccg ctctgtcctg
    15721 gtcatgacca gagaggtaca gcccgtaagc gtaaacctgc tccgggtcgt tcggcttctg
    15781 ctgcgccaga ttgcgcatta acgtatcggc ctggctgcgt tgtccggcct gccagagatc
    15841 ctgcgaaagt cggtaagtaa tccatacgct gccggggtcc agcgccagtc gttgccgctg
    15901 aagtgctgcc gcctgcgccc atttgccctg gttttccagt gcctctgcct gctgtgccag
    15961 acggtcgttt tgcaggctgc gttcgatatc atcaatgcta cgccgctgac tggcagagag
    16021 cgaggcgata aacgcttcag ctttttctgg cgattgctgg cggtaagtat ttgccagccc
    16081 gcgcacggcg ttagtgttgc cgctgtccat acgcaaggtc tgctgataat aacgttcggc
    16141 ggcgggataa tcttttcgcg ccatcgccac atcgcccagc cccagcactg cataactgtc
    16201 ggtgttatcg acattacgcg cctgctggaa caggcgttct gcccggtcag gattattggc
    16261 tttcagcgca gcatcgccct gctggatcgc cagccagtag cggtttactt tcagcagact
    16321 gttccatttg tcgttgttgc tgctgtgcgg gtccagtgcg agggcttttt ccagattcgc
    16381 cactgcattg gcgcgatcgc ctttctgaga atacgcctgg cccagcgccc ccagagcttc
    16441 actgtctttc gggttcgccc gcaccgcctg ttgtagttcg ggaatggctt tacccgccat
    16501 accagagtcc accgccgcta aaccttgcgc acgagcgcgg aaagcaggat cggccagctg
    16561 tttttgctgt tctgccagtt gcgattgcgc agccgccacg ctatcgccat cactaaagat
    16621 cgagagatat tttttcagcg ccgacacact ggcatcactg acgggcatgt ctttaatctg
    16681 cccgtaccag attttagagg ccccttcgcg cccggcgttc gattttgcca tctgttccag
    16741 gacggcaaaa ccttcgtcac ggcgatcgct actaaacagc aatagcgcca gattgttttg
    16801 caggcccgta ttgcccggtg catccgcatt gatgcgtttt aactgattaa tcgcttcgcc
    16861 acggcgagcc ggaattttcg ccaccgtact ccagtactcg acagcaatgt caccttccgg
    16921 cggcgcaccg ttgaacagtt tgttgtaact cgccacagct tcttctgcat gaccggtcgt
    16981 cgcctgcaat cgtgcctgtt gcagtgcctg acgaccatcc ggcgtggaaa gtagcatcgt
    17041 agtccgcgac gatttatacg catttgaact cggcgctaac tgcgacagcc gatcgagctg
    17101 tttttgcgcg ccatcaatat cgccctgacg taacaaagaa cggaaacggg cggcaacgac
    17161 gtccgggtta ttcggatcaa taagttccag ccgatataac gactgttgca ccagatcttc
    17221 acgatgggtc gcttcgccta accgaacttg ctccagcaac tgttgctgag cggttggtgc
    17281 tgcctcgacc atcggcatga cggccagacc gagggaaagc gtgaatatgt ttagtgtgaa
    17341 tttgcgcatt cctggcccca gtcaggtaat aactcacctt ttgtcgagaa gcggaaacgg
    17401 tgttgatccc agccttgtcc aaacagggtc agcacatagt tgtaataggc atcgctgccg
    17461 ggaaagttat cggccacgcg ctggcgctga acggcctgcg catcgcggtt ttgtaaaaag
    17521 ggcagcatgg cggcagaaaa accgactggt cctttaccct gcgctttccc cgtagccaca
    17581 tccacttttt ccggcggata accgtttttc tcagtgaatg tcgccatcgg tttaaaccgg
    17641 ttgagcatcc gcgctttctg cggatcgctg tcaggcatca tgcctaccca catgtaaacg
    17701 cggatggcgt cgtagctgct gatcaatgtt ttttcggctt ttagctgcca gcctttgtct
    17761 ttctcataac gcacccagtc tggcgaaaag cctttcgggg cggtttccag caataaacgt
    17821 tgattggttt cgcgcagcgt agtccacggc gcgccaaagc gggtgaaata ctgcgccagc
    17881 gtcggcggca ggtagctggg gttaaaacgc cagctgttat cctcagcaaa acccactttg
    17941 cccggtaaca acatggaacc cagcccaggc accgtcacca cttcctcccg cgcgatacgt
    18001 tttagcaacg cgctgccgat gtcggtataa cgctgctctt tccacaaacg ccccgcctcc
    18061 agcaacgacc aggccatcca gacatcacca tcggaggccg aattgctgtc cagcacttcc
    18121 cacttactgt tctctttctt gccccacagc caggcgggca aacgttcttt taaagaaccc
    18181 tgagcgagat tgttctgcgt ccagtcgaga atattatcga aagctgcacg gtcgttagcc
    18241 gccagggcaa agaacatacc gtaactttgc ccttcggagg tggtgatttt gcgcgcgtcg
    18301 ctggggtcga tgacgcgccc ttcctgactg atgtaatcct ttttaaactg ctcccaggca
    18361 ggccaggtac aggctgcctg aacactaaag gcagccaaca acagcatcgt cacgattcca
    18421 ctacgcaaca cattcatctt caattactcg ttatccgggt taagacgacg acgactaata
    18481 attcgcagca gacgccacag tacccatgcc agcaatatca cactgatagc cgccagcacc
    18541 gccagcagaa tcggatggtt tgccagcgca taccacacgc gctcgaacca cggcagatga
    18601 cctacgtaat aaacgtcgcc aacacgtagg ctgttgatac cggactcgcg gatcaccgcg
    18661 accgaaccga acatggtggc gcgtttgccg ctatcgttca ccgcatcgtt aagcatttca
    18721 taaccgcgtg ggctatctgc caacagcgca atcacgctgc gctggtcgtt atacggcgac
    18781 tggaagccaa tcaccgccgc catcgcaccg gaagaggtca gcgttgaccg ggtttctgcc
    18841 gcgcgatcgc tctcgtccgg cacaatgccg gggaacgggg tctggcgcat cggtgttttc
    18901 acccagcttt cggtcgcctg caccaatagg tcgatctgct tatcgtcttt cagtttgtcc
    18961 gggataccac cgatgatcat gatgtcggca tctttgccct gaatggtgct gccatcatcg
    19021 gtcaccgtca gattaatcgc cgggaagccc gtctgtgcgc cgataaaacc aacagtattc
    19081 agcaacgttt ccatctgtgc ttcgttaggc gctttcggca tcacggtgat ggtttgcgac
    19141 agatccgcca tccggctgaa tgggaagccc gcgttagcaa aggcgcgtag atccggcatc
    19201 gggatgaagt ggtaatactt cgagaagtcg atggtggagt cgtcaccaat caccacatga
    19261 ttctgcaccg gctggaaggt aatacagtta tccaccgaac cgcccggcat cgggttcata
    19321 tactcaaagt cgaagcgcag ctggttggtc gcgcccagtt tcagcgccgg aatagagaca
    19381 tctgttttgc catccagcaa accttgtaat accggaatcc gcagcagcag gcggttcgcc
    19441 tcctgtttgc tgctcaggtt gaaggattgc aggaactggt tattcaggct gatatccatc
    19501 cgcgaactgt ctttcaccgg cggcatggtg tagcggtaat taatatccat atcaatgccg
    19561 gtactgcgca tcaggtagag atccggcggc aggtttagcg aaacgttaat cgctgctggc
    19621 tcaagaccgc tggattgtaa ctgttcttca taggttttca gttcgccaaa tgtgaccgga
    19681 cgatcggtac gtacccagtt cggcgcatcg tacggcttac gcggtagcag cggtttcact
    19741 tcattcacta ccacgctttc accacggaac agaatgttac cctgagcgat acctttcgct
    19801 gcctgcaaca ggtctttgtc gtcacgacca aacaccacca gcagtttgac gtaaggattc
    19861 tgcggatggt tgatcatctc aatcaccggg gcttttaccg ccggatgatc gcgcaggaag
    19921 tccggccgtt tgtcgttggt tgcaaagaca atggcattac gatccggcag ttggttatag
    19981 agcaccggga agttctgccc acgccagcca gaacgcgaac caaaccacga ggcgacaatg
    20041 gcagatgctt gttgcagccc aacatccggc gcacccgcaa agaccatcgg caaggtgtta
    20101 gtacggttat cgcgcgggtc aaagaatggc accgggaagt gtgacaggtc attcttcaca
    20161 ttcagggtct gataggtcag atccagtcca ctgctccgcc caacatccag ccaaagcgtg
    20221 gtgctggccg ggttttcgca cacgtcctga taatggccga caaactccag ccgcacacgg
    20281 ttgaagtcgg taataaacag tgggttaatg ggcatttgcg ccagcgtttt tttacccaac
    20341 tgttctttgg tcactggcag tacgcccatc agctcatcat tgagataaac ctttaactgc
    20401 gactggacag gcagtaacga tggcgatggc atgtattcga ggttgagcat cgccttcgtc
    20461 accacttcat cgctgcgcat accaaactca atgctgccgt tcgggttaat gccacgtagc
    20521 accatgctgc ccggcggcgg cgcaatttgc gcaaaggtca gcttcacatc acgcgaagga
    20581 ccgttctgcg ccacgactgg cgcatcagcg ccctgcacgc ccggcatcac ttgccccacc
    20641 tgcggatttt gttccgtctg ggcgactaca gctggctcag cattgatcag tggttgcgtt
    20701 gctggcgtcg cctgcgtcat gaaagagggg aacgcactca tccccatagc cactgcacaa
    20761 atccagaata gttttctttt catcgcgtta tcatcattgt tgagccaaag cctgatccga
    20821 tggttgtgcc gtttcgctcc gctccgggcg gcgcagaata aacgatacaa cccaggaaac
    20881 cagagaagtc agcacacgga atatgccctt caccgaagaa ggcgcaaact ccgccagatg
    20941 gcggtagcca cggaagccga gcttcagaat atccagcaga ctttccagcg gcttatcttc
    21001 cgggtagctg tcctgccaga gcgcccatgt atccgcacgg gcaaacgtac actgcacaaa
    21061 atcgatatgt tgctgggtgg tgagcggcat taatttcagc ccaacttcat tacccatcac
    21121 gcgcgccacc tgggtcggga agacgtattc ctgctgaccg cgtttaagca acagattcac
    21181 tttctgccct tccagaatct gcgcctgacc gttgatcttg atccccaaac caccgtcgga
    21241 gaaatcctga acggtacacg aaaagaggtg accatcttcg cgggcaattg ccgcgggcat
    21301 cgtcatctcc acgcggtgcg atcggcgtac ctgtttgctt tctaccgata ccgcaactgc
    21361 gccgccaaga acaatcaggt tgtagaacac ccacaccata ctgacgacca cggtgagcat
    21421 ctcggttggc gggccataga agtagcgcca gatgccgacc gcaacaccca ccaggttgag
    21481 cagaacaagg aagatgtagg gccgcgagat cacccagtcg acgtactctt cttccaccag
    21541 tccacctttg gcggtgacgt taaatttgcc tttgtgcggg ttaatcagcg ccaccagcgt
    21601 cggtggtgcg atataccacg ccagcaccgt ttcgtagatt tcactccaga aagagtggcg
    21661 atatttgccc tggatcttcg agttggtcag gctggcatgg atcatatgcg gcagcacgaa
    21721 cagggcgatc atcaacgctg gcgcatagat gatgtaggca tgaagcagca ggaacgccag
    21781 cggcgcagtc aggaagatca gtcgtggaat gcccgacaag aagtggaaca tggcgttgac
    21841 gtagcacagc cgctgggcga atttcagccc tttaccggta agcgggttat cgagacggaa
    21901 gatttgcacc atcccgcgtg cccagcgaat acgctgaccg atatgcgccg acagactttc
    21961 ggtcgccagc cccgccgcct gcggaatacg catatatgca gaggtgtagc cacgacggtg
    22021 caggcgcaga gacgtgtgcg catcttcggt taccgtttcg acagcaatac cgccaatttc
    22081 atccaatggt ttgcgacgaa ttactgcaca ggaaccgcag aagaaagtgg cgtcccacat
    22141 atcgttgcca tcctgcacca gaccatagaa cagcgtgcct tcgttcggcg ttttacgaaa
    22201 acgccccagg ttgcgttcaa acgggtccgg cgagaagaag tggtgcggcg tctgcatcat
    22261 cgccagctgt ttctctttca ggaaccagcc catggtcatt tgcaggaacg atcgcgttgg
    22321 tacgtggtcg cagtcgaaaa tcgatacgaa ctcgcctttg gcatatttca gcgcattgtt
    22381 gatgttgccc gctttcgcat gttcatgagt ggtgcgggcg atatatttca cccccacgtt
    22441 ttgcgcaaac tggcgaaact cttccctgcc gccgtcatcg aggatccaga tgttcagttt
    22501 gtctttcggc cagtcgatac ccagcgaggc gtaaatggta tttttcacca cgttgagatc
    22561 ttcgttgtaa gtcgggacaa agatatccac cgacggccac agcgacatat ctttcggcaa
    22621 tggcaccggc tgacgattca gcggccatac gacctggaag tagccgagca ccagcacaat
    22681 ccacgcgtac gtttcagcga agagcagaat aagcccgcac accaggctga ccggatcgtc
    22741 ccagttcagc gtagaggtgt aacgccacca gatataacgg caagaaacgg tcagcgacag
    22801 cacaatcaac attagcgccg agaagcgccc cggcatccgc cgtacgatca gcgctacacc
    22861 ccacagcagc atcaggaaga taaactgcgc cagcgggtta aacggttgag taacgcagat
    22921 taacgccaga atcagcgaga aggtgacgat gataccgagg atcaaccgcc gcgccccggc
    22981 actcaaatga ccgagctctt ttttctcatc aagatgctgt gttttatgac taacgcgctc
    23041 aggcagctcg ttcatccatt gatggtaacg tccacgaata ttctgcagac ctgaaaatgc
    23101 ccgcctgcgc ggtttcggcg tttctttgcg cgatgtaccg atcagtaacc agcatgtttg
    23161 aatgaaataa cggaccgggt ccagcggacg cggacgcgag gcgttgatat gcggatacag
    23221 gtttttatgt tctgcgcgaa tacgctgcca gcgcgggtgc tccagcggaa taaaaatcca
    23281 ggccaggatc atccagaaac agccgagcgt cgcgctgaaa gccgacgcac cgtgacgacg
    23341 ataatcgcga taacgcccga taagccgcgc gttgaccggc gggataagca accaccgggt
    23401 caggatactc atgatgcact cccgactggc gttttcagcc cggagtagtt caacaggcac
    23461 cagttcgcca gcgtcagtat ctcttcagcc gccagcgcat cactgcgata ttcacctact
    23521 ggttgcttag ccgccaggca ttcagccatc gcttcatcac gatgaatgag catcggcagt
    23581 aatcggcgct ggctttgcaa ccaaagctgg taaatatcgt cctgaacctg actgccaata
    23641 cggaagtcat taatcaaaat atgtgcgcca tccggcagcg cttgctgatg cagtcggata
    23701 tggcagttgg catccacatt gacgattgcc agcgagtgat cgcacaaact cagcagctgg
    23761 tgggttatct gcgaggcatc acgcggtaag tcgattaaaa tccacaggta acgcccgctg
    23821 gcttttagtt gctgtaagcc ggagcaaata tcgctcagcc gggtttgcca gtgctgtgga
    23881 ttttcttgtt cttcaatgga taactgacca aaaggcagca aatcgagctg cgaggtgtag
    23941 cgcaacccag cgtcacgcca gtcctggcca tccagcatcg ctctggccca gccctgacgg
    24001 tgggtaaaat caacattaaa tgacaggcgc aacaagttgt ccgggcaggc atcgaccacc
    24061 aggacatttt ctcccaacat ttgtaatgac caggctaatg cggcggtgat ggttgttgtc
    24121 cccacgcctc cccgcacccc ctgcaatccc agtacggcca ttcaccaatc ccctactttt
    24181 gttgcgcaaa ctctgccagc aacggccagc gttttaatgc cgcggccaac tgttcgcgtt
    24241 gggaaatatc ggcataatca atatcaggca gtgaaaatgc ctgctttaac gccacaatat
    24301 cattctggaa gatatagcct atcgcgggat caggcagagt atctggttca ttgttattca
    24361 ttttgcttga tccttatgaa ataaccgtac cgttcatgag acaattctaa ccgtgagcat
    24421 ttcgccggtt tattgtttac ggcgagacgg gaatttgctc actttttgac tttccgttac
    24481 gcaacagaaa aagttaaaaa cgaaaagact aaaatcacac gtcttgtgac cttctaaatt
    24541 gattcacatg ctaaatctga taagttttaa tttcaatggt aggtttattt cttagctttc
    24601 gctagtaaac tgataatcag acaaaatggt gacatgaggg acattgtggg ccctgtattc
    24661 tctatcggta tctcatcatt atgggatgag ctgcgacata tgccagcagg cggcgtctgg
    24721 tggtttaacg tcgatcgcca tgaagatgct atcagtctgg cgaatcaaac aattgcatcc
    24781 caggctgaaa ccgcacacgt cgcggtcatt agcatggaca gcgatccggc gaaaatcttt
    24841 caattagatg attatcaagg gccggaaaaa ataaaattat tttcaatgct aaatcatgaa
    24901 aaaggtctat actatttgac ccgtgatttg cagtgttcta ttgatcccca taattacctt
    24961 tttattcttg tttgcgcaaa taacgcatgg caaaacattc ctgccgagcg gcttcgctca
    25021 tggttggata aaatgaataa atggagcagg ttaaaccatt gttcgctttt ggtaattaat
    25081 cccggaaata ataacgataa acaattttca ttgttgcttg aggaataccg ttcacttttt
    25141 ggtcttgcca gtttgcgttt tcagggtgac caacatttgc tggatattgc cttctggtgc
    25201 aacgaaaaag gggtcagcgc ccgtcagcag cttagcgttc agcaacaaaa tggtatctgg
    25261 acattagttc aaagcgaaga ggcggagatc caaccacgca gcgacgaaaa acgcattctg
    25321 agtaatgttg ctgtactgga aggtgcgccg ccgctatcgg aacactggca actgttcaac
    25381 aataacgaag tcctgttcaa tgaagcccgt accgctcagg cggcgacggt ggtcttttct
    25441 ttacagcaaa atgcgcaaat cgagccactg gcccgcagca ttcataccct gcgtcgccag
    25501 cgcggtagtg cgatgaaaat cctcgtgcgg gaaaataccg ctagcctgcg cgccaccgat
    25561 gaacgtttgt tattggcctg cggtgcaaat atggttattc cgtggaatgc gccactctcc
    25621 cgttgtctga cgatgatcga aagcgtgcaa gggcagaagt ttagtcgcta tgtgccggaa
    25681 gatatcacta ccttgctgtc aatgacccag ccgctcaaac tgcgtggttt ccagaagtgg
    25741 gatgtgttct gtaatgccgt caacaacatg atgaataacc ctctattacc tgcccacggt
    25801 aaaggcgttc tggttgccct acgtccggta ccgggtatcc gcgttgaaca agccctgacg
    25861 ctgtgtcgcc ctaaccgtac cggcgatatc atgaccattg gcggtaatcg gctggtgctg
    25921 tttctctcat tctgtcggat taacgatctg gataccgcgt tgaatcatat tttcccattg
    25981 cctactggcg acattttctc aaaccgtatg gtctggtttg aagatgatca aatcagtgcc
    26041 gagcacgatg gtcgccactg gcgacgaata ccagaaccca tgcgactgtt agatgatgct
    26101 gtggagcgct catcatgatg accatcagcg atatcattga aattattgtc gtttgcgcac
    26161 tgatattttt cccgctgggc tatctggcgc ggcactcttt gcgacgcatt cgcgacacct
    26221 tacgtttgtt ctttgctaaa cctcgttatg ttaaaccggc cgggacatta cgccgcacgg
    26281 aaaaagccag ggcaaccaaa aaatgactca atttacgcaa aataccgcca tgccttcttc
    26341 cctctggcaa tactggcgcg gcctttccgg ctggaacttc tattttctgg ttaagttcgg
    26401 cctgttgtgg gcgggatatc ttaacttcca tccgctcctc aatttggtgt ttgccgcgtt
    26461 tctgctgatg cccattccgc gctacagcct gcatcgcttg cgccactgga ttgccctgcc
    26521 gatcggcttt gctttgttct ggcatgacac ctggttgcct ggcccggaaa gcataatgag
    26581 ccagggttcg caggtggcgg ggttcagtac cgattattta atcgaccttg tcacacgctt
    26641 tattaactgg cagatgattg gggtaatgcc gatcagttaa ggatcagttg accgatccag
    26701 tggctgtgta agaatccgga aacgctcact tgtttccgga tttttttatg cacat
//
LOCUS       DAJTMK010000041        26294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTMK010000041 DAJTMK010000000
VERSION     DAJTMK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26294
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.042"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(68..715)
                     /gene="ychE"
                     /locus_tag="OO135_001461"
     CDS             complement(68..715)
                     /gene="ychE"
                     /locus_tag="OO135_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5397367.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1193..3868
                     /gene="adhE"
                     /locus_tag="OO135_001462"
                     /gene_synonym="adhC"
     CDS             1193..3868
                     /gene="adhE"
                     /locus_tag="OO135_001462"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5397368.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVLKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYTDQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLLSELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3992..4888
                     /locus_tag="OO135_001463"
                     /pseudo
     CDS             3992..4888
                     /locus_tag="OO135_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5170..5787)
                     /gene="tdk"
                     /locus_tag="OO135_001464"
     CDS             complement(5170..5787)
                     /gene="tdk"
                     /locus_tag="OO135_001464"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5397369.1"
                     /translation="MVQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6383..6796
                     /gene="hns"
                     /locus_tag="OO135_001465"
     CDS             6383..6796
                     /gene="hns"
                     /locus_tag="OO135_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5397370.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6940..7848)
                     /gene="galU"
                     /locus_tag="OO135_001466"
     CDS             complement(6940..7848)
                     /gene="galU"
                     /locus_tag="OO135_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5397371.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8050..9063)
                     /gene="rssB"
                     /locus_tag="OO135_001467"
     CDS             complement(8050..9063)
                     /gene="rssB"
                     /locus_tag="OO135_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5397372.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9155..10060)
                     /gene="rssA"
                     /locus_tag="OO135_001468"
     CDS             complement(9155..10060)
                     /gene="rssA"
                     /locus_tag="OO135_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5397373.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10173..10631
                     /locus_tag="OO135_001469"
     CDS             10173..10631
                     /locus_tag="OO135_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5397374.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFISFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10681..11523
                     /gene="purU"
                     /locus_tag="OO135_001470"
     CDS             10681..11523
                     /gene="purU"
                     /locus_tag="OO135_001470"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5397375.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSALLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11683..11767
                     /locus_tag="OO135_001471"
     tRNA            11683..11767
                     /locus_tag="OO135_001471"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11717..11719,aa:Tyr,seq:gta)
     gene            11815..11946
                     /locus_tag="OO135_001472"
     ncRNA           11815..11946
                     /ncRNA_class="other"
                     /locus_tag="OO135_001472"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12130..12807)
                     /gene="narI"
                     /locus_tag="OO135_001473"
     CDS             complement(12130..12807)
                     /gene="narI"
                     /locus_tag="OO135_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5397376.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12807..13517)
                     /gene="narJ"
                     /locus_tag="OO135_001474"
     CDS             complement(12807..13517)
                     /gene="narJ"
                     /locus_tag="OO135_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5397377.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13514..15052)
                     /gene="narH"
                     /locus_tag="OO135_001475"
     CDS             complement(13514..15052)
                     /gene="narH"
                     /locus_tag="OO135_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5397378.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15070..18792)
                     /locus_tag="OO135_001476"
     CDS             complement(15070..18792)
                     /locus_tag="OO135_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5397379.1"
                     /translation="MSKFLDRFRYFKQKDETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            complement(19308..20699)
                     /gene="narK"
                     /locus_tag="OO135_001477"
     CDS             complement(19308..20699)
                     /gene="narK"
                     /locus_tag="OO135_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5397380.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            21038..22834
                     /gene="narX"
                     /locus_tag="OO135_001478"
     CDS             21038..22834
                     /gene="narX"
                     /locus_tag="OO135_001478"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5397381.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            22827..23477
                     /gene="narL"
                     /locus_tag="OO135_001479"
     CDS             22827..23477
                     /gene="narL"
                     /locus_tag="OO135_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5397382.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(23478..24872)
                     /gene="ychO"
                     /locus_tag="OO135_001480"
     CDS             complement(23478..24872)
                     /gene="ychO"
                     /locus_tag="OO135_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5397383.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGRNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKSGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVAEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            25056..25409
                     /gene="ychN"
                     /locus_tag="OO135_001481"
     CDS             25056..25409
                     /gene="ychN"
                     /locus_tag="OO135_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5397384.1"
                     /translation="MQKIVIVANSAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(25453..26148)
                     /gene="chaC"
                     /locus_tag="OO135_001482"
     CDS             complement(25453..26148)
                     /gene="chaC"
                     /locus_tag="OO135_001482"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5397385.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
BASE COUNT         6691 a         6907 c         6354 g         6342 t
ORIGIN      
        1 gcgggaacaa agtaaaaata atgacgaagt aaacggagca tggcagctcc gtttcatttg
       61 aaaggaatta attaagcagg ccggggaaaa tccccttaat accagtaacg ataaattcaa
      121 tccccaatgc catcagcaat agccccataa tacgcgtaat cacgttgatg ccggtctggc
      181 gtaaaacccg taccagccaa ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca
      241 atgcaatagc cacaaagaaa ccaaacagat agctaatgct gtgataacgc gtaccccaga
      301 cgatggtaga actgatcgcc cctggccccg ccatcaacgg caacgccaat ggcaccacac
      361 caatgctttc acgtaccgcg gtttctgatt tttcttgctt gttctgttta tcctcgccaa
      421 gcttgccgct gatcatcgac atcgctatcg tcaccaccag gatgcccccg gcgatacgga
      481 acgaatcaat tgatatacca aaaagttgca gaatcgtgtc gccgagaaaa agcgagatcc
      541 acaagataat ggccacagac aggttggctg taaggttagt tttgtttcgc gctgccgctg
      601 tctgataact ggtcatgctg ataaagacgg gaataatccc taccgggttg accagcgcaa
      661 ataacccgat gaaaaatttg aagtaaacgg gaaaatcaaa aaaggtctga atcacggtta
      721 gctccgaagc aaaagccgga taatgttagc cataaataag gttgaaaaga cgcgctgaca
      781 atacgccttt tgacagcatt tttcacctcc taaatactta aaattgctat cattcgttat
      841 tgttatctag ttgtgcaaaa catgctaatg tagccaccaa atcatactac aatttattaa
      901 ctgttagcta taatggcgaa aagcgatgct gaaaggtgtc agctttgcaa aaaatttgat
      961 ttggatcacg taatcagtac ccagaagtga gtaatcttgc ttacgccacc tggaagtgac
     1021 gcattagaga taataactct aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc
     1081 gcgagtaagc ttttgatttt cataggttaa gcaaatcatc accgcactga ctatactctc
     1141 gtattcgagc agatgattta ctaaaaaagt ttaacattat caggagagca ttatggctgt
     1201 tactaatgtc gctgaactta acgcactcgt agagcgtgta aagaaagccc agcgtgaata
     1261 tgccagtttc actcaagagc aagtagacaa aatcttccgc gccgccgctc tggctgctgc
     1321 agatgctcga atcccactcg cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga
     1381 agataaagtg ctcaaaaacc actttgcttc tgaatatatc tacaacgcct ataaagatga
     1441 aaaaacctgt ggtgttctgt ctgaagacga cacttttggt accatcacta tcgctgaacc
     1501 aatcggtatt atttgcggta tcgttccgac cactaacccg acttcaactg ctatcttcaa
     1561 atcgctgatc agtctgaaga cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa
     1621 agatgccacc aacaaagcgg ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc
     1681 gaaagatctg atcggctgga tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca
     1741 ccacccagac atcaacctga tcctcgcgac tggtggtccg ggcatggtta aagccgcata
     1801 cagctccggt aaaccagcta tcggtgtagg cgcgggcaac actccagttg ttatcgatga
     1861 aactgctgat atcaaacgtg cagttgcatc tgtactgatg tccaaaacct tcgacaacgg
     1921 cgtaatctgt gcttctgaac agtctgttgt tgttgttgac tctgtttatg acgctgtacg
     1981 tgaacgtttt gcaacccacg gcggctatct gttgcagggt aaagagctga aagctgttca
     2041 ggatgttatc ctgaaaaacg gtgcgctgaa cgcggctatc gttggtcagc cagcctataa
     2101 aattgctgaa ctggcaggct tctctgtacc agaaaacacc aagattctga tcggtgaagt
     2161 gaccgttgtt gatgaaagcg aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat
     2221 gtaccgcgct aaagatttcg aagacgcggt agaaaaagca gagaaactgg ttgctatggg
     2281 cggtatcggt catacctctt gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc
     2341 ttacttcggt cagaaaatga aaacggctcg tatcctaatt aacaccccag cgtctcaggg
     2401 tggtatcggt gacctgtata acttcaaact cgcaccttcc ctgactctgg gttgtggttc
     2461 ttggggtggt aactccatct ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac
     2521 cgttgctaag cgagctgaaa acatgttgtg gcacaaactt ccgaaatcta tctacttccg
     2581 ccgtggctcc ctgccaatcg cgctggatga agtgattact gatggccaca aacgtgcgct
     2641 catcgtgact gaccgcttcc tgttcaacaa tggttatact gatcagatca cttccgtact
     2701 gaaagcagca ggcgttgaaa ctgaagtctt cttcgaagta gaagcggacc cgaccctgag
     2761 catcgttcgt aaaggtgcag aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct
     2821 gggtggtggt tccccgatgg acgccgcgaa gatcatgtgg gttatgtacg aacatccgga
     2881 aactcacttc gaagagctgg cgctgcgctt tatggatatc cgtaaacgta tctacaagtt
     2941 cccgaaaatg ggcgtgaaag cgaaaatgat cgctgtcacc accacttctg gtacaggttc
     3001 tgaagtcact ccgtttgcgg ttgtaactga cgacgctact ggtcagaaat atccgctggc
     3061 agactatgcg ctgactccgg atatggcgat tgtcgacgcc aacctggtta tggacatgcc
     3121 gaagtccctg tgtgctttcg gtggtctgga cgcagtaact cacgccatgg aagcttatgt
     3181 ttctgtactg gcatctgagt tctctgatgg tcaggctctg caggcactga aactgctgaa
     3241 agaatatctg ccagcgtcct accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt
     3301 tcacagtgca gcgactatcg cgggtatcgc gtttgcgaac gccttcctgg gtgtatgtca
     3361 ctcaatggcg cacaaactgg gttcccagtt ccatattccg cacggtctgg caaacgccct
     3421 gctgatttgt aacgttattc gctacaacgc gaacgacaac ccgaccaagc agactgcatt
     3481 cagccagtat gaccgtccgc aggctcgccg tcgttatgct gaaattgctg accacctggg
     3541 tctgagcgca ccgggcgacc gtactgctgc taagatcgag aaactgctgg catggctgga
     3601 aacgctgaaa gctgaactgg gtattccgaa atctatccgt gaagctggcg ttcaggaagc
     3661 agacttcctg gcgaacgtgg ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg
     3721 cgctaacccg cgttacccgc tgctctccga gctgaaacag attctgctgg atacctacta
     3781 cggtcgtgat tatgtagaag gtgaaactgc agcgaagaaa gaagctgctc cggctaaagc
     3841 tgagaaaaaa gcgaaaaaat ccgcttaatc agtagcgctg tctggcaaca taaacggccc
     3901 cttctgggca atgccgatca gttaaggatt agttgaccga tccttaaact gaggcactat
     3961 aacggcttcc acaacaggga gccgttttct tatgccactt ctcaatgatc tgctcgattt
     4021 cagtgaccat ccgcttatgc ctccgccctc tgcacaacta tttgcagaac accttcccac
     4081 cgagtggata caacactgcc tgacgctttc tgctcatgcg accgttcgcc gccgtcgttt
     4141 accgggggac atggttatct ggatggtggt gcaatgagcc aattaccgat gttgttcgcc
     4201 gtctgaacct gagcgcggat ggcgaagcgg ggatgaacct gctggcccgc agcgctgtca
     4261 cccaagcgcg tcagcgcgtg ggggccgccc cagtggaatg gctcttccgc cagaccgcac
     4321 agacaggggc gcggaacgtt acctgaagga tgactggcac ggcctgcaac tttttgccat
     4381 tgatggcgca cagttcagga cacctgataa acccgagctg cgtgaatatt atgggtctgc
     4441 caacacatcc actaagcggc agaacgccta cccggttatg cgtctggtgg ccttaatgaa
     4501 cctgggaagt cacattctgc tgaatgccgt gaccgcacct taccggcaga gcgaaactgt
     4561 gctggcccac tccatgctcg ccaccatccc ggataactct attacgttgt ttgataagat
     4621 tttttacagc gaagacctgc tgctaacgct gaaccagaag ggttgtaacc gccactggct
     4681 actgcctgcg tggaagaata tcgcatcaga aatgatagag ctgggaaaca cggcgtcacc
     4741 agggaccatc ccgaaacgac tggagcatct tcggggtgct ctggaagtgg tgttcataac
     4801 aaaacgcccc cgaccatcaa ggccgaggtc ggttaagata tcaaaaaccc gttatcctgt
     4861 gaaacatagt gccgctcctc ttaagtgaac ggcattgccc ttctggggcc gtattttttg
     4921 tttacccaaa gcaacttttc cataaaccga cagcattagc cttcatcata tttgcgacga
     4981 tgtataacgc ctaaacacag ggatattgta ctttacaggt cacaagtcaa cgtcggtgct
     5041 taagagccct gtgaggcgta tagcggcgtt aaaaaactgc cgagaagggt atatagcccg
     5101 gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc tgctcaccct gtcagtaaag
     5161 aaattcttat taatcgtggc gatgcctttc ctgaatagcc gttaatgagc cgacttgtaa
     5221 cgcctcttta tagtgtttac ggcatacaga aacgtatcgt tcattaccac caattaccac
     5281 ctgctcacct tcgttataag gtctgcctgc ttgatcaaga cgcagcacca tgcttgcttt
     5341 acggccacaa aaacagatgg tttttaattc aaccagtttg tcggaccatg ccagtaagta
     5401 ttggctgcca ataaataatt cacctcgaaa atcggtacgt aaaccataac aaagtacggg
     5461 tatatcgagt tgatcgacaa cctccgataa ttcatatact tgttgtctgg ttaaaaactg
     5521 gcattcatca accagtacgc aatgaattgc ctgctgttca tgttccgcac gaatctcatc
     5581 aaataatgat gaattttggt taaataattt tgcaggcgat gacaaaccta tacgcgaact
     5641 gactttcccg gcaccaaagc gatcatcaat ttctgccgta tatacgacag tgcgcatgcc
     5701 gcgttcttgg taattgtatg aagattgcaa caatgctgta gacttacccg cattcattgc
     5761 ggaatagtag aaatatagct gtaccatcag ccacaggccc tcaatgatat gaaataaagt
     5821 tggctggagt ttattcagtt tttcagctca acagtatgca cagaagtggg tatatgcgtt
     5881 ctcccttacg aagccttgca taatccttct gagctatcat tacaactgcc tcgcttgtta
     5941 taagcgggta aatgactgct ggtaactatt cacaaccttt aacctgttgc gcaagtaata
     6001 gccctctgtt gacctccagg agatagtgca atactaagtc catgctctta ttgcgactgt
     6061 tctacttttc atcattcgct taatagggaa ttctcgtaaa cacaactaat acagaagact
     6121 gaaaggtcgt cagcctacga taatctcccc ataaaatgtg acatgaatca ggaagtttta
     6181 acctcacgtg ctgcgaaatc atcggtgtaa atagggctat atgccgcgtc ttttctggct
     6241 aattttatga aaagatattt attggcggca caaaataaag aacaattttg aattccttac
     6301 attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca aaccacccca
     6361 atataagttt gagattacta caatgagcga agcacttaaa attctgaaca acatccgtac
     6421 tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt
     6481 agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga
     6541 gcgcactcgt aaactgcagc aatatcgcga aatgctgatc gctgacggta ttgacccgaa
     6601 cgaactgctg aatagccttg ctgccgttaa atccggcacc aaagctaaac gtgctcagcg
     6661 tccggcaaaa tatagctatg ttgacgaaaa cggcgaaact aaaacctgga ctggccaggg
     6721 ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt
     6781 cctgatcaag caataatctt ttgtagattg caattgctta aaatcccgcc atcggcggga
     6841 ttttttattg tccggtttaa gacaatttaa taagaataag ctataaaaaa acggcgtcga
     6901 ttgttcaacg ccgtttcgtg gataacaccg atatggatgt tacttcttaa tgcccatctc
     6961 ttcttcaagc caggctttaa attccgtgcc aagggtgtta tgacgaatac cgtattcaac
     7021 gaaggcctgc atgtaaccta atttattacc gcagtcatgg ctcttccctt tcatatgata
     7081 ggcttccacc gtttcttttt cgatcagcat atcaattgcg tcggtgagct gaatttcatc
     7141 accagctccc ggaggggttt ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg
     7201 acccacaata gcgagattag acggcgcaac atccgctttc ggtttttcta ccacaccaac
     7261 catcggtaca ctttcacccg gcgctaattc aacgcctttg caatccacaa cgccatatgc
     7321 ggtcacatca gcaacctgtt caaccatgat ctggctatga cccgtttcat caaagcggcg
     7381 gatcatctct gccaggttat cctgtgacaa atcggattca tattcatcca gaataacatc
     7441 aggcaaaata acagctaccg gttcatcacc cactaccggg tgagcacaca acaccgcgtg
     7501 tcccaggcct ttcgccagcc cctgacgaac ttgcataata gtcacgtgcg gcggacaaat
     7561 agactgcact tcatcaagca gttgacgttt tacacgtttt tccagcattg cttccagttc
     7621 aaaactggta tcaaagtggt tttcaatcga gtttttagat gagtgcgtaa ccagcacaat
     7681 ttcagtaatg ccagccgcaa tacattcatt cacgacgtat tgaattaatg gcttatcgac
     7741 aagtggcagc atctctttcg ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc
     7801 cgcaacgggg ataacggctt ttttgacttt cgtattaatg gcagccattt aaattctcct
     7861 ggactgttcg tgttttgaac gtgttcatat ttctgtatcg catcccagta tatcagcacc
     7921 ccgaaggggc taagttctga aaaggaggcg agttagcata attaagacaa ttacttatac
     7981 gaatggcgac gatagtacca ccagccgaca ttagcaggta atgcaaattt agtccgcgtt
     8041 atcgtttgct cattctgcag acaacatcaa gcgcagtcga ccaccggttc cccatatttg
     8101 gcattgccag gcatcgcatc gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc
     8161 taacggaaca ccattactga tttgcacctg gtgttcgccg gtatttaacg tcgcattcag
     8221 acccgcagaa accagaatga gatttttcag ttcgcgatga taatagccaa ctaatagcgg
     8281 aaactgcccc ggcagattgg cctgacggag taaatggttt acctgcttca ataacgcgcc
     8341 cagctctggc aaccgttgat tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa
     8401 tgcgcgtaat aacaaggcag caagtacgcc attatgtcca gctcgggtga catcaaggca
     8461 ataaaatgcc agatcgtttt ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc
     8521 cgcggcaacc aattgacgat aattaacccg gcaatgggaa atcacctgct gaaccggcgg
     8581 ttgtagttcc tgtaataatt tcgccgctgc ggcagggtta tcaaccgttg catcccaatc
     8641 gcgaaaaagc ctttcctctt cctcaacgcg cgaattaaac atgctgggat agagacaggc
     8701 aaaaaccatc tcgcgcaagc gattcagatc tttaactggt ttcagcaaaa catcttcaac
     8761 gcccagacgt aacgctttgg caatatctgc catattttca gtggcagata tcaccagaac
     8821 tggggtctgg tcgcctctgt tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg
     8881 catcgcgata tcacatatca tcaggtctgg agtgaaacct cccagcaact caagggcatc
     8941 caccccatca gccgccagta ccgttgtcgc tcccaatgag gaaaaccatg aatccagaag
     9001 cgagcgaaat acctgctcat cttcaacaat gagaatctgt tttccgacca atggctgcgt
     9061 catgttctct cccctgactg gctttactca atagtggcat gctattgcca cctgcgcctg
     9121 tcagaattaa cttaagtgta gataaaaatt ctggtcaaat gttggtgcgt accaacggca
     9181 aaagttcgtc cattttcctt tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc
     9241 gatggaaatc aagcgtagat atttgcgggc aaacaggttg aatcagaata tcgggcggat
     9301 cacctgccat gcggttcctt ttaaggcggt tctccagcac ctggatagaa gtggtcataa
     9361 tctctgttgc cgttggcgct gtcaccgccc gacgtgttgt tatactgcct aacctttctt
     9421 tcagacgcgc atgccacggc aaagattcac cattctcgct attttcttca ctgacattaa
     9481 aggagagcaa atcttgttgc atcaaatgag catcgtgctg caggtcgacc gctatcacaa
     9541 tatcagcccc caatgcacgc gtgagggaaa taggaattgg gttaactact gctccatcaa
     9601 ccagccagta gccgttatgt gcaacaggtg ccatgagtcc tggaatactg catgatgcgc
     9661 gaatagcaag atggagatcg ccttcagtaa accataattc acgtcccgta cttaaattgg
     9721 tggcaacagc cgcaaagcga cgggaacaat tttcgatctc tgtttccggc attatttcgc
     9781 ggtattgatt gaagacacgc tcgccgcgca gtaacccacc gcgctgccag gagagatcca
     9841 tcaggcgtaa aacatcccaa taactgaaag aggtcaccca atcttccagc gcagataatc
     9901 gatcgcatgc ataggcagcg cccaccagcg aaccaattga acatcctgca acgatatcaa
     9961 tttcaatacc cacttttttt agcgcattaa taacgccaat atgcgaccaa cctctcgccg
    10021 cgccagatcc cagcgccagc cctattttta tctttctcat tattcccgca agattcccct
    10081 ggaatgcaat cgtagccaca tcgctactgc tcggttaaca tagtgctact caggcggtta
    10141 atacgccgtt attgtttcca gggagattat ttgtgtctca gctttgtccc tgtggtagtg
    10201 ctgtcgagta tagcctatgt tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc
    10261 cagaacatct catgcgttcg cgttactgcg cttttgtgat gcaagacgca gattatttaa
    10321 taaagacctg gcatccctct tgtggagcag cggcgttacg tgccgaattg atggccggat
    10381 ttgcgcatac tgaatggttg ggattgaccg tatttgaaca ttgttggcag gacgccgaca
    10441 atatcggatt tatcagtttt gttgctcgat ttaccgaagg tggcaaaaca ggtgcaataa
    10501 ttgaacgttc gcgatttttg aaagaaaacg gtcaatggta ctatattgac ggtacacgtc
    10561 cgcagtttgg tcgcaacgat ccctgccctt gtggttcagg taaaaaattt aaaaagtgct
    10621 gcggccaata atggttgacg gtacggttta gcaaacactc tcaacaaggt ttttccagca
    10681 atgcattcac tccaacgtaa agttctgcgt actatttgtc cggaccaaaa aggtctgatc
    10741 gcacgtatta ccaatatttg ctacaagcac gagttaaata tcgtacagaa caatgaattt
    10801 gttgatcacc gtaccgggcg cttttttatg cgcacggaac tggaagggat ttttaatgat
    10861 tccgccctgc tggcggatct cgatagcgca ttgccagaag gctccgtgcg tgagctgaat
    10921 cctgccggtc gtcgccggat agtgattctg gtcactaaag aagcgcattg ccttggcgat
    10981 ttgttgatga aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac
    11041 cacgatactt tacgttctct ggttgagcgt tttgatattc cgtttgagct ggtaaaccat
    11101 gaagggttaa gccgcaacga gcacgatcaa aagatggcag acgccattga tgcttataaa
    11161 cctgactatg tggtgctggc gaagtatatg cgggtattaa cgccggaatt tgtggcacgc
    11221 ttcccgaata aaatcatcaa tattcaccat tcattcctgc ctgcgtttat cggcgcacgt
    11281 ccttatcacc aggcctatga acgtggtgtg aagattattg gcgcaaccgc tcactatgtg
    11341 aatgacaatc tggacgaagg cccaatcatc atgcaggacg ttattcatgt cgatcatacc
    11401 tacacagctg aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt
    11461 gcgctataca aagtactggc acagcgcgtc tttgtttacg gtaatcggac gattattctt
    11521 taatcgccag caaaaataac tggttacctt taatccgtta cggatgaaaa ttacgcaacc
    11581 agttcatttt tctcaacgta acactttaca gcggcgcgtc atttgatatg atgcgccccg
    11641 cttcccgata agggagcagg ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg
    11701 ccaaagggag cagactgtaa atctgccgtc atcgacttcg aaggttcgaa tccttccccc
    11761 accaccatca cttttcaaaa gtccgtaaac tctcaaacga atccgcaatt aaatattctg
    11821 cccaagcggg gaaggatgag aagcttcgac caaggttcga cttgagcgcc agcgagagag
    11881 cgttgccgca ggcaacgacc cgaagggcga agcgcgcagc gctgagtaat ccttccccca
    11941 ccaccatcgc ttccttgaat aatcaaaacc tgttactccg agctaatttt gcccgatggc
    12001 accacgttta ccgggcctac aatatcctcc cctgtaggtc ggataagcga agcgcatccg
    12061 acaactcacc ccgcaccgtg cccataaaaa aaccccgccg aagcagggtc tatgtgaact
    12121 aaaattcgtt tagtgacgag cgcgcaccag ctggtactta cgtgtcagat actccaccgg
    12181 tacgctccag atgtgtacca gacgcgagaa cgggaacagc aggaataacg tcatccccag
    12241 caccaggtgc agacggaaga taaacgccac accatcgagg tgttgagaag cgccaccgtg
    12301 gaaggtcacc accgactgcg cccagccaac cagtttcatc atctcgctac cgtccatatg
    12361 ctgagcggag aacggaatgg tcaacaggcc cagcgcgcac tggataacga gcagcgacag
    12421 gatcaggata tccgctccgg tagtggttgc acgcacgcgt gggctgaaca gacgacgctt
    12481 cagcaacagc acgccgccaa tcagacacag cacgccgctg gcaccaccag caaacatcgc
    12541 cattttctgt ttcacttcaa tcggcagcca ggcttcatac atccagtgcg gcgtcagcat
    12601 accgaagaag tgaccaacaa aaatccccag aataccgata tggaacaggt tcgacgccag
    12661 gttcatccct ttgcgatcca gcatctggct ggacgccgcg cgccaggtgt actgcccgta
    12721 gtcataacgc agccagctac caatcaggaa gaccgccccg gctatgtacg ggtagatatc
    12781 aaagaagaac atattcagga attgcattag tgctgtcctc cggtggtgat attcagatat
    12841 tgcggcgcga cggcaccggc aaagcgacgc tgatgagcag tgattgctga atcaccgcag
    12901 cctttgtcag caaagaattt aacctgctct tcttcccaga cagcatccag cgcctgcggc
    12961 gtatcatcgc gcgcttcgtc ggcaattttt tccgccactt tgtcgctgtc gatagcggta
    13021 ttggccaatt tcagcagcag atcaaacatc acggcataac ggctttcacg ctgttgcaga
    13081 cgcgcgctca gcaatgccag aatcggcgcg atatctttca aaccttccac ggcttcgctt
    13141 tgcggcagct gcgacaggta ctccagatac agcggcagat ggtccggtaa ttcgcggctg
    13201 tttaactgca agccgtgctg ctcgtactgc gccagcaggt ccaccatcgc ctggccgcgg
    13261 tcacgggatt caccgtgaac atgttcaaat agcaacagtg acgtggcgcg gccacggtcg
    13321 aacagttcgc tgtactgggc ctgggcatcg agcggatcca tcgccgttaa atcgcgcagg
    13381 aaaatgccca gcgcatgggc atcttctttc gacagatttt tcgacgcagc aatcgcctca
    13441 aacatctctt gttgatgctg ccataaggca gcatccggat attcaaggag acgcgataca
    13501 atcacgagtt cgatcatgga tgcggctccg ttttgctggt cacatcgatg gcatcgatac
    13561 gacggctgtt gaacagattg aatttggtat ctgagccgtg gcagccatca ccaaaggtaa
    13621 agccgcagcc atttttctcc gggaaggctt cccgtgccag ttcacgatga ctactcggca
    13681 ccacaaagcg atcttcgtag ttagcaatcg ccagataacg gtacatctcc tgtgcctggg
    13741 cttcggtcag accgacctct tccagcgcac gggtatcaac tttaccgtca acggtttcag
    13801 cacgtttgta atgacgcatc gccagcatac gtttcagtgc gcgcagtacc ggtttggtat
    13861 caccggcggt cagcagattc gccagatact gtaccggaat acgcaagctt tcgacgtctg
    13921 gcagaatgcc gttgctaccc agctcacccg cgtctgctgc agactgaatc ggagacagag
    13981 gcggcacgta ccagaccatc ggcagtgtgc gatattctgg atgcagcggc agcgccagtt
    14041 tccattccat tgccatttta taaaccggcg actgctgtgc ggcttcaata acgctcagcg
    14101 gaataccgtc tttaatcgcc tgctcgatga ctttcggatc gttcggatcg aggaacacct
    14161 ccagctgacg ctggtaaaga tctttctcgt tctcggtgct ggctgcacgt tcaatcgcgt
    14221 cggcatcgta cagcagcacg ccaagataac ggatacgacc gacacaggtt tctgagcaca
    14281 cggtcggctg acccgcttca atacgcggat agcagaagat gcacttctca gacttaccgc
    14341 tcttccagtt gaagtagatt tttttgtacg ggcatccggt gatgcacata cgccagccac
    14401 ggcatttatc ttggtcgatc aggacgatgc catcttcttc acgcttgtaa atcgcaccgc
    14461 tcgggcaggt cgccacacat gccgggttca ggcagtgttc gcacaggcgc ggcaaataca
    14521 tcataaaggt gttttcgaac tggctataca tcgccttctg gatgttgtcg aagttcttgt
    14581 ctttcgccag tttgtcaaac tcaccaccca ggtcatcttc ccagttcggc cctttttcga
    14641 ttttcgccat ccgttccccg gtaatcagcg aacggggacg ggcaatcggc tgcgatttgc
    14701 tgccttccgg cgcggtatgc aggttctgat agtcaaaatc gaacggctcg taataatcgt
    14761 cgatccccgg cagatgcggg ttagcgaaga ttttacccag caacatggca cggttaccca
    14821 tgcgcggctg cagtttgccg ttgattttac ggatccagcc gcctttgtat ttttcctggt
    14881 tttcccagtc agtcgggaag ccctgacccg gcttggtttc cacgttgttg aaccacgcgt
    14941 actccacgcc ttcacggctg gtccagacgt ttttacaggt aactgaacag gtgtggcagc
    15001 cgatgcactt atcgagattc agcaccatgc cgacttgtga acgaattttc attttacgct
    15061 ctcctgtacc tagtcattgc cttcgccatc taaccagtca atgttcttca tcttacgcac
    15121 tacaacaaac tcatcgcggt tagaacctac ggtgccgtag tagttaaagc cgtatgccag
    15181 atgggcatag ccaccaatca tatgcgtcgg tttcggcgtg atacgggtga ccgagttgtg
    15241 gatcccccca cgctggccag taatttccga acccggcagg ttaacgatac gttcctgcgc
    15301 gtggtacatc atggtcatcc ctgccggaac acgctggctg acaaccgcac gggcagtcag
    15361 cgcaccgttg ctgttgtaaa cttcaatcca gtcgttatcg gcaatgccca actctttggc
    15421 atcggcttcg ctcagccaga ccaccgggcc accgcgacct aaggtcagca tcaactggtt
    15481 gtcgctgtag gtggagtgga tcccccactt ctggtgcggc gtcaggaagt tgagcgcttt
    15541 ttccgggttg ccgttggatt tctggcctat cacttctttc accgaacggg tgtcgatcgg
    15601 cggacgataa accagcaggc tttcaccgaa atcacgcatc cactggtgat cctgatacag
    15661 ttgctgacga ccagagagcg tacgccatgg gatcagctcg tgaacgttgg tgtaaccggc
    15721 gttgtaagaa acgtgttcat cttccagacc agaccaagtc gggctggaga taattttgcg
    15781 cggctgtgcc tgaatatcgc ggaagcggat cttctcgtct tctttattca gcgccagatg
    15841 cgtatggtca cgaccggtaa attcgctcag ggcagcccag gctttcacgg ctacctgacc
    15901 gttggtttcc ggtgccagtg tcaggatcat ctctgccgca tcaattgcgg tgttcagcat
    15961 cggctggcct ttcgccggac cttccgcttt ggtgtagttg agcttacgca gcagatccat
    16021 ctcgctctgg gtgttccagg caatcccttt accgccatta ccgattttct ccatcagcgg
    16081 gccgatagag gtaaagcgtt cgtaagtcgc cggataagcg cgctctacga ccataatgtg
    16141 tggcgcggtt ttacctggga tcaggtcgca ctcgcctttt ttccagtctt tcacatccag
    16201 cggctgcgcc agttcagcgg cagagtcatg ctggataggc agcgtgacga tgtcggtttc
    16261 tttacccaga tggccgacgc acacttcgga gaatttcttc gcgatggctt tgtagatttc
    16321 ccagtcgctt ttcgcttccc aggccggatc gaccgccgca gacagcgggt gaataaacgg
    16381 atgcatatcc gaagtattca tgtcgtcttt ttcgtaccag gtcgccgtcg gcaaaatgat
    16441 gtcggaatag agacaggtgc tcgacagacg gaagtccagc gtaaccacca gatccagctt
    16501 gccttccaga ccattgtcct gccagtccac ttcttccggc ttcacgccgc cctgttgccc
    16561 cagatcttta ccctggatac cgtgctccgt ccccagcagg tacttgagca taaactcatg
    16621 acctttaccg gaagaaccga gcaggttaga acgccagatg aacaggttgc gcgggtggtt
    16681 tttaccgttt tctggttgtt ctgccgcaaa acggatggaa ccctctttca gggatttcac
    16741 cgtatagtcc accggattca tcccggcttt ttccgcttcg ccagcgatag tcagcgggtt
    16801 agtgcctaac tgcggtgcag acggcagcca gcccatgcgt tccgcacgga cgttaaagtc
    16861 gatcaagtgt ccggtatagc gggatttgtc cgccatcggt gacagcaact cttccgccgt
    16921 gacggtttca taacgccact ggctggagtg gttatagaaa taagaagtgc tgttcatgtg
    16981 acgcgccgga cgctgccagt caagggcaaa cgccagcggc tgccagccgg tttgcggacg
    17041 cagtttttcc tgacctacat agtgcgccca gccgcccccg ctctgaccga cacagccgca
    17101 gaaaatcagc atgttgatca gaccacgata gttcatatcg aggtgatacc agtggttcag
    17161 ccccgcacca acgataatca tcgaacgacc gtgcgtttta tcagcgttat cggcaaattc
    17221 acgggcgatg cgaataatct ggctgcgaga aacgccggta atctgctcgg cccaggccgg
    17281 ggtataagct ttcacatcgt catagctggt tgcacagtta acgtcgttca ggccacgttc
    17341 cagaccgtag tttgccagcg tcagatcata aacggtggtc accagggcgg tgctgccatc
    17401 agccagttgc aggcgtttca ccggcagttt gtgcagcagc acgttttcca gttccacttt
    17461 gttgaagtgt tccgtgccgt cgccaccaaa gtacgggaag cctacctcag cgatctcatc
    17521 ttggctaccc agcaggctca gttgcagctc ggtttcttcg ccagttttgc cgtcgcgctg
    17581 ctcaagattc catttgccct tctcgcccca gcggaagcca atagaaccgt tcggcgcaac
    17641 catttcgcca ttggtattaa aggcgacagt tttccattcc ggattgtttt cctggcccag
    17701 cgcatcaacc agatcagcag cgcgcagcat acgacctgca gcgtagtaac cgtcgcgttc
    17761 ttccagcatc accagcatcg gcatgtcggt gtagcgacgc acatagtcgg tgaaatactg
    17821 gcttgggttg tcgaggtgga attcacgcag cattacgtgg cccatcgcca gcgccattgc
    17881 cgcatcggtg ccctgtttcg gtgccagcca cagatcgcac agtttggcga tttcagcgta
    17941 gtctggtgtg acggcaacag ttttggtgcc tttgtaacgc acttcagtaa agaagtgagc
    18001 atccggggta cgcgtctgcg gcacgtttga accccaggcg atgatgtagc tggagttata
    18061 ccagtcagca gattccggta cgtcagtttg ctcgccccag gtttgcggag acgcaggagg
    18121 caggtcgcag taccagtcgt agaagcttaa gcaagtaccg ccaatcagcg agagatagcg
    18181 tgcacccgat gcgtaagaaa ccatcgacat tgccggaatt ggcgagaaac cagcaacacg
    18241 gtccgggccg tagtttttga tggtgtaaac gttagatgcg gcgatcagtt cgttcacctc
    18301 ctgccaggaa gaacgaacaa atccaccgcg tccacgcgcc tgcttaaagc ttttcgcttt
    18361 atcggcgtct tcaatgatag aagcccatgc ctcaaccgga tcgctatgca gcgccttcgc
    18421 ttcacgccac attttcatca ggcgtttgcg catcatcggg tatttcaggc ggttggcact
    18481 gtaaagatac caggagtagc tggcaccgcg cgggcagccg cgaggttcat ggtttggcag
    18541 atccggacgg gtacgcggat agtcagtctg ctgggtttcc caggtgacca gaccgttttt
    18601 gacgtagatt ttccagctgc aggagccggt gcagtttacc ccgtgggtag agcggacgat
    18661 tttgtcatgc tgccaacgct ggcgatatcc atcctcccag tcacggttgg tattgagaag
    18721 ctggccatgc ccatcggcaa aggtttcatc cttctgtttg aagtagcgaa accggtccag
    18781 gaatttactc atcggttttc tcctgtggga gcctgtcggc cctctgataa atcgacattg
    18841 ctaatgttta agggcaaggt aacgctctga aacagcgtgg gaattgataa cgatcaagag
    18901 tgatggggat aaaaaataaa gtaaattcct aagatacccc tttaggataa ttaacagcca
    18961 ccacataaca gactgtaaat taagacgttt tatacctgta gttatttaaa ctattaagat
    19021 ttttatgcag atgggtatta aggagtattc cccatgggta acatattaat ggctcttaat
    19081 gaaatccggc gaaataaacc atataacatt agtatgttgt aactaaatag cgcttaaaaa
    19141 aagcgcgaca tcatgccgcg caaaggataa tcaaatattt tactctatat aattcggttt
    19201 acaggaaggc tgcaagtgaa tgaatcccag gagcttacta aagtaagtga ctggggtgaa
    19261 tgaacgcagc cgcagcacat gtaacccgaa gtatgacgag taacggttta ttttttagaa
    19321 tgccgaccat ataccgccca ggtaatcact acgcaggcga tatagaaaat caaaaatacc
    19381 ttcattgcgc cgactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    19441 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgccgc cgtgtcggtt
    19501 gccgcttcac gcatcgcacg ttcgtcagac cccccttctg ctttcacgcg atccattgtc
    19561 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    19621 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    19681 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    19741 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    19801 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    19861 agcattgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    19921 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    19981 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    20041 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    20101 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    20161 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    20221 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    20281 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    20341 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    20401 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    20461 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    20521 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    20581 atccacaggt tacggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    20641 ggtcgccaat ctgtaatgac agctccagta gccctttcgg gggcggatga gtgactcata
    20701 gacacctctg atactcgttt cgatgtctgc caccttagtg tctgtagcta aaggcaattt
    20761 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    20821 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtaaaa tccctaccct
    20881 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc gggtatgggt
    20941 atacttcagc caatagccga gaatactgcc attccagaat gtatcgtcac attcattaag
    21001 gttattgctc atttaaagcc tgaaggaaga ggtttacatg cttaaacgtt gtctctctcc
    21061 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    21121 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    21181 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagtga
    21241 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttaactcg
    21301 agcagcagaa cgagacgaac aactggcgca attacagggt ttacaagatt actggcgtaa
    21361 tgaactgatc cctgcactga tgcgtgcaca aaaccgcgaa acggtgtcag cggatgtcag
    21421 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    21481 cattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    21541 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    21601 ggcgagtgcc gtcagtcatc gcgattttac ccaacgcgcg aacatcagcg ggcgcaacga
    21661 aatggcgatg cttggaactg cattgaacaa tatgtctgca gaactggccg aaagttatgc
    21721 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    21781 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    21841 gtcacctgta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    21901 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    21961 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    22021 aaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    22081 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    22141 actcaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    22201 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    22261 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag caagcagccg
    22321 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    22381 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaggcgag
    22441 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    22501 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    22561 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    22621 tcaggtcaaa ctgaccgtcc aagataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    22681 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    22741 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    22801 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    22861 tcgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    22921 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gagtctcttg
    22981 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    23041 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    23101 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ttatctgtta aaagatatgg
    23161 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    23221 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    23281 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    23341 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    23401 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    23461 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    23521 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    23581 gccaccaccg acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    23641 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    23701 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    23761 ggcactgttt cgccagattt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    23821 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    23881 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    23941 ccaaagcggt aattaagatt ccgcccgagg ttattttgat tctcgccact ttcaccctgt
    24001 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    24061 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    24121 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    24181 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    24241 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    24301 cccgctcgct gaagattttc gtccagcaag ttgtcataaa aagtgttata acccaccagc
    24361 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    24421 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    24481 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    24541 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    24601 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    24661 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    24721 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    24781 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    24841 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    24901 aatcatagcc ttaaagaata acgattactg gcaacttttg tcgtattttc tcgcgctttc
    24961 aggaaaatcc ttaaaaatgt gaccgattgg tcagacgctg cagaagcagt aagatagaag
    25021 atgagtgtct aattccctga atacaggagt aaataatgca aaaaatcgtg atcgttgcca
    25081 atagcgcacc ttacgggagc gaatccttgt ttaacagctt gcggctggcc attgcgttac
    25141 gagagcagga gagcaatctg gatctgcgtc tgttcctcat gtctgatgcg gtcacagccg
    25201 ggttgcgcgg gcaaaaacca ggggaaggct acaacattca gcaaatgctg gagatcctta
    25261 ccgctcagaa tgtaccggtg aaattgtgca aaacctgtac cgacgggcgc gggattagta
    25321 cacttcctct gattgatggg gtggaaatcg gtactcttgt ggaactggcg caatggacgc
    25381 tgtcagccga taaagtgctc acattttaat gtttgagggg gcgtctgacg ccccactatg
    25441 cgggaagttt actcaggcga atcccggccg taacacacca tccggataat tctccgccag
    25501 cagttttttt accgatacca gcaaatcatt cagcccgtcg tcctgcattc ccagtttgat
    25561 aagctcctgt tccagtgaaa acaggtattg tgcgttggtt cccagcggac cgctcgccgc
    25621 cgcaatcaac ggcgcgatga cctgagcgcg agtgtcagat tcatattctg gatgtcgcgg
    25681 gtccataata aacacaatgg cgtttactgt gcatccatca tcaagatcaa gctgacacca
    25741 ggttggcaga taacagccgg taatcatctc gcgcttccac aacagggtta gttcctgctc
    25801 cagcgtctct tctggcagtc gataggcgac gcctgtagtg cgtccgccct ctttcagtgc
    25861 aagcatccgt ccaggctggt gcgcagtccc gcgcccggcg gtcaggcgca ggcaaaacgc
    25921 gcgatgccat ccaaccagtg taccggtgca cgactcggta aactccagtg ccggattcca
    25981 catcaacgat ccataaccga agatccacac cggtccctca tcaggtcgac aagccagcgt
    26041 cgccgccagc gaagccgctc gttgctctgc tgaccataag agtgattctt caatggcacc
    26101 aaatgccgtt ttacaatcgg cattcatcaa gaaatcacgc gttatcacct tacacctccg
    26161 ccactgcata cttccctgcg acaacttttt ggtgtcttcc ggacattttt actgctcatt
    26221 ttgaaaccac taacgggaca atatgcaatc cacggaccac ttgcaaggca acaccgcacg
    26281 aatagcttta acta
//
LOCUS       DAJTMK010000042        25354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTMK010000042 DAJTMK010000000
VERSION     DAJTMK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25354
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.043"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(275..745)
                     /locus_tag="OO135_001483"
     CDS             complement(275..745)
                     /locus_tag="OO135_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5397386.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLALAESTS"
     gene            complement(861..1412)
                     /gene="maa"
                     /locus_tag="OO135_001484"
     CDS             complement(861..1412)
                     /gene="maa"
                     /locus_tag="OO135_001484"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5397387.1"
                     /translation="MSTEKEKMIAGELYRSADETLSCDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1584..1802)
                     /gene="hha"
                     /locus_tag="OO135_001485"
     CDS             complement(1584..1802)
                     /gene="hha"
                     /locus_tag="OO135_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5397388.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1828..2202)
                     /gene="tomB"
                     /locus_tag="OO135_001486"
     CDS             complement(1828..2202)
                     /gene="tomB"
                     /locus_tag="OO135_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5397389.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2748..5897)
                     /locus_tag="OO135_001487"
     CDS             complement(2748..5897)
                     /locus_tag="OO135_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5397390.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5920..7113)
                     /gene="acrA"
                     /locus_tag="OO135_001488"
     CDS             complement(5920..7113)
                     /gene="acrA"
                     /locus_tag="OO135_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5397391.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            7255..7902
                     /gene="acrR"
                     /locus_tag="OO135_001489"
     CDS             7255..7902
                     /gene="acrR"
                     /locus_tag="OO135_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5397392.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            8030..11392
                     /gene="mscK"
                     /locus_tag="OO135_001490"
     CDS             8030..11392
                     /gene="mscK"
                     /locus_tag="OO135_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5397393.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLEQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11604..11765)
                     /gene="rsmS"
                     /locus_tag="OO135_001491"
     CDS             complement(11604..11765)
                     /gene="rsmS"
                     /locus_tag="OO135_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5397394.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11779..12306)
                     /gene="priC"
                     /locus_tag="OO135_001492"
     CDS             complement(11779..12306)
                     /gene="priC"
                     /locus_tag="OO135_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5397395.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12376..12762
                     /locus_tag="OO135_001493"
                     /pseudo
     CDS             12376..12762
                     /locus_tag="OO135_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
     gene            12915..13466
                     /gene="apt"
                     /locus_tag="OO135_001494"
     CDS             12915..13466
                     /gene="apt"
                     /locus_tag="OO135_001494"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5397396.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13595..15526
                     /gene="dnaX"
                     /locus_tag="OO135_001495"
     CDS             13595..15526
                     /gene="dnaX"
                     /locus_tag="OO135_001495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5397397.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    14859..14923
                     /gene="dnaX"
                     /locus_tag="OO135_001495"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15579..15908
                     /locus_tag="OO135_001496"
     CDS             15579..15908
                     /locus_tag="OO135_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5397398.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15908..16513
                     /gene="recR"
                     /locus_tag="OO135_001497"
     CDS             15908..16513
                     /gene="recR"
                     /locus_tag="OO135_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5397399.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16623..18497
                     /gene="htpG"
                     /locus_tag="OO135_001498"
     CDS             16623..18497
                     /gene="htpG"
                     /locus_tag="OO135_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5397400.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18678..19322
                     /gene="adk"
                     /locus_tag="OO135_001499"
     CDS             18678..19322
                     /gene="adk"
                     /locus_tag="OO135_001499"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5397401.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            19454..20416
                     /gene="hemH"
                     /locus_tag="OO135_001500"
     CDS             19454..20416
                     /gene="hemH"
                     /locus_tag="OO135_001500"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5397402.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVMPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(20413..21372)
                     /gene="aes"
                     /locus_tag="OO135_001501"
     CDS             complement(20413..21372)
                     /gene="aes"
                     /locus_tag="OO135_001501"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5397403.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPRLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21524..22828
                     /gene="gsk"
                     /locus_tag="OO135_001502"
     CDS             21524..22828
                     /gene="gsk"
                     /locus_tag="OO135_001502"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5397404.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22961..24637)
                     /locus_tag="OO135_001503"
     CDS             complement(22961..24637)
                     /locus_tag="OO135_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5397405.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24875..>25354)
                     /gene="fsr"
                     /locus_tag="OO135_001504"
     CDS             complement(24875..>25354)
                     /gene="fsr"
                     /locus_tag="OO135_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5397406.1"
                     /translation="SYYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIG
                     RKYVIWGSILGVEPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIG
                     MVSGLFFGFAFGMGGLGAAVLGLIADHTRIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6345 a         6585 c         6518 g         5906 t
ORIGIN      
        1 atcacttatt taagtgatac tggttgtctg gagattcagg gggccagtct acagcaatac
       61 tgaaagaatc agtactcccg aaataaggcc gactagatgt cgtgttctca ccagaatatc
      121 cttattaaca cagtgtaggg aaagtatatc tgattgtgga aaaaggctgg caggagataa
      181 ggaggtagag ctggtatttc atgaagtgat gggtggaaac gatcattgtc atttgaccaa
      241 gcgcagagct ttacgcctgg tatgtaaccc taacttaaga tgttgattcg gcgagagcga
      301 gggtaaaaat atcgtaaaaa gacagttcac ctttacggac gatcattttt tgcagttgtt
      361 ccttatgaac atccgcgaca cgcggagagt tcaagatggc ttcaaccagc gcggcaggta
      421 caaagcgcgt gttgtaccat tcgccgttat agcaaacgcg gaaatccagc acgtcgatat
      481 cttcatagcg gtagcgtggg tagacatcga aaaagaagaa accattaagc aggataaata
      541 atacaaccag tagccagaca gagcctgaca gcgtttcgga ctgcaacatc accgccaggg
      601 tggcaaaaaa agcaacgtac ataccgataa acagccccgg atgtttacgg ataaaactga
      661 tactaaagcg gggtttgttg tcgcgttttt cgcgggtatt cagagactca atcgtttcgg
      721 tcagcaggcg ttgtatttcg gtcatcgttt gccttcgtgg tatctgcaac tttccaggga
      781 acaccctatt ttaacgtggg tatagtagag gggaagtaac agatctacca caattttgca
      841 taacagttgc gaaaaaccga ttacaatttt ttaattattc tggctggatt accgcccacg
      901 acaacgttgt ccgggacacc ttttgtgaca actgcacctg aagctaccac gacgttatca
      961 ccaatggtca caccagggtt aatgaccgcg cgtccgccaa tccagacgtt attaccgatg
     1021 gtgacgggtt tccccagttc agcaccgcta ttacgtgcta cagggtcgat ggggtgtgtt
     1081 gccgtgtaga tatgaacgcc tggtgccaac atacagttat caccgatgcg aatagggcag
     1141 acatcaagca tcacgcaatc gaagttggcg aaaaaattat tgccgagaaa aatgttatag
     1201 ccataatcac agcgaaacgt tggctcaata taagcctctg tcacctgacc gaataaatca
     1261 gcgagaattt gctggcgtaa tgtgtgctct tccgccaggg aatgattgta tcggtgaata
     1321 agctgacgag cgcgcaggcg atcgcaagat aacgtctcat ctgccgagcg atacaactca
     1381 ccagcgatca tcttttcttt ttctgtgctc attactgacc tccggttgct gaaaatgcgg
     1441 caacacagta agacgaaaag tcagtgaaca gaacgttccc attacatctg tgtgataaag
     1501 atcacatagg gtatgtcttc atggtgaaaa agtataaaat tcttaataaa cagccggtta
     1561 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
     1621 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
     1681 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
     1741 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
     1801 atacttctac ccatggttgt aataatacta acaagataaa ctcgcaggat tctctttcgt
     1861 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
     1921 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
     1981 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
     2041 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
     2101 ataattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
     2161 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
     2221 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
     2281 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
     2341 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
     2401 caggtaaaag cagtttgctc gaactgttca taacatttca ggattatcgc cagatgaaat
     2461 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
     2521 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
     2581 gctacttaat ataaattaac taacaatgag tattttttat gaattttatc cgtggttaat
     2641 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
     2701 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
     2761 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
     2821 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
     2881 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
     2941 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
     3001 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
     3061 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
     3121 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
     3181 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
     3241 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagaggcgt
     3301 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
     3361 tgttccatta gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
     3421 tccatggatg gcaggccatt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
     3481 gaggagaatg ccgagaacgg caccatctga ccatcagcag cacgaacata ccagtcgccg
     3541 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
     3601 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agtggtgtta
     3661 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
     3721 tgcggggtat cttccagacc gtttggacgt acgctggtca acatatcagg atgctttgct
     3781 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
     3841 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
     3901 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
     3961 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
     4021 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
     4081 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
     4141 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
     4201 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
     4261 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
     4321 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
     4381 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
     4441 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
     4501 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgctga cagtaccatc
     4561 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
     4621 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
     4681 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
     4741 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
     4801 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
     4861 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
     4921 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
     4981 attgccgcag cggtatccag cgcatttgca ccggtcgcca gcttgatccc cagaccggaa
     5041 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
     5101 gccacatcac gcagcaggac gcgggaacca tcctgattca ctttcagcag gattttgccg
     5161 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
     5221 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
     5281 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
     5341 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
     5401 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
     5461 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
     5521 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
     5581 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
     5641 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
     5701 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
     5761 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
     5821 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
     5881 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
     5941 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
     6001 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
     6061 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
     6121 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
     6181 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
     6241 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
     6301 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
     6361 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
     6421 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caacgtagat
     6481 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
     6541 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gcggtgactt tggtgtaagc
     6601 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
     6661 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
     6721 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
     6781 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
     6841 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
     6901 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
     6961 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
     7021 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
     7081 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
     7141 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
     7201 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
     7261 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
     7321 cttttctcac agcagggggt atcatccacc tcgctgggtg agattgcaaa agcagctggc
     7381 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
     7441 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
     7501 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
     7561 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
     7621 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
     7681 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
     7741 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
     7801 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
     7861 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
     7921 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
     7981 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
     8041 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
     8101 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
     8161 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
     8221 caaactggag cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
     8281 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
     8341 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
     8401 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
     8461 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
     8521 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
     8581 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
     8641 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
     8701 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
     8761 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
     8821 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
     8881 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
     8941 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
     9001 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
     9061 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
     9121 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
     9181 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
     9241 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
     9301 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
     9361 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
     9421 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
     9481 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
     9541 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
     9601 gggctggctg aaagcgtatc aacaaaaact ggcttccgct gtgggttccc tgcgtaacga
     9661 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
     9721 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
     9781 gctatggtcg ttcagcaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
     9841 ggtactggag aagaacggcg ttgccgtgcg tcacttcggc atgccggaac agcagaccag
     9901 ccactggcgt cggcaaattg tccgcatcag tctcgcattg ctgcctatcc atttctggtc
     9961 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aagcgatgat
    10021 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    10081 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    10141 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    10201 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    10261 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    10321 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    10381 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    10441 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcattac
    10501 gctctggcat tacaacggta ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    10561 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    10621 tggtttgctg gaagtgctgg tgctctcgcg actgaatatg cgccagggcg cgtcgtatgc
    10681 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    10741 gctgggcgtc tcttgggata aactccagtg gctggcggca gcattatccg taggtcttgg
    10801 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    10861 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    10921 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    10981 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    11041 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    11101 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    11161 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    11221 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    11281 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    11341 ggaagtaaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    11401 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    11461 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    11521 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    11581 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    11641 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    11701 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttccagt
    11761 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    11821 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    11881 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    11941 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    12001 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    12061 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaactgc
    12121 atgacgaagc gcagccagat tatcgcccgc ctcatcgaga caagcctgca gtgttgtcgc
    12181 acgggtttga aaaaggtgtc tgtcaaaacg ggcacttagc gtggcaaatt gcgctaccgg
    12241 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    12301 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    12361 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    12421 ctgggtacgc tgggcgtggt attaccggta ttaccggtat taccgacgac gccgtttatc
    12481 ctgctggcgg cctggtgctt tgcccgttct tccccgcgct ttcacgcctg gttgctgtac
    12541 cgctgatggt ttggcagcta tctacgtttc tggcagaaac atcatgcgat gccgcgcggc
    12601 gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg ccatttctct gtggttcgtc
    12661 cagatgccat gggtgcgcat catgttgctg gtaattctcg cctgtttgct tttctatatg
    12721 tggcgaattc cggtgattga tgaaaagcaa gaaaagcact gaagcacaac aatcgcagtt
    12781 gcaattattg cgtacagcca gtacattctg gcgttttcga gcacaggcgc aggcggtcaa
    12841 aggttaaaca actgttactt ttgatacgtt taaaacgcgc cgtgagtacc accgtaacaa
    12901 gcaggcatac acttatgacc gcgactgcac agcagcttga gtatctcaaa aatagcatca
    12961 aaagcattca ggactaccca aaacccggca ttcttttccg cgatgtcacc agcttactgg
    13021 aagacccgaa agcttacgct ctcagcatcg acttgctggt tgagcgttac aaaaatgcgg
    13081 gcattaccaa agttgtcggc accgaagcgc gtggcttctt gtttggcgct ccggtagctc
    13141 tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg caaactgccg cgtgaaacca
    13201 tcagtgaaac ttacgacctg gaatacggca ccgatcagct ggagatccac gttgatgcca
    13261 tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct ggcaaccggc ggcactatcg
    13321 aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt ggctgacgct gcgttcatta
    13381 tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa acagggcatt accagctaca
    13441 gccttgtccc gttcccgggc cattaattat cgccagtctt gtgctgccca cgctacggac
    13501 agcacaagat gtgcattcag cctcgccgtt ctgacggggc tgtgttagca ttaccccttc
    13561 gtgaatccac cttcctgcgt ttcagagcct gccaatgagt tatcaggtct tagcccgaaa
    13621 atggcgccca caaacctttg ctgacgtcgt cggccaggaa catgtgctga ccgcactggc
    13681 gaacggcttg tcgttagggc gtattcatca tgcttatctt ttttccggta cccgtggcgt
    13741 cggaaaaacc tctatcgccc gactgctggc gaaggggcta aactgcgaaa ccggcattac
    13801 cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc gagcaggggc gctttgtcga
    13861 tctgattgaa atcgacgccg cctcgcgcac caaagttgaa gatacccgcg acctgctgga
    13921 taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt tatctgatcg acgaagtgca
    13981 tatgctgtcg cgccacagct ttaacgcact gttaaaaacc cttgaagagc cgccggagca
    14041 cgttaagttt ctgctggcga cgaccgatcc acagaaattg ccggtgacga ttttgtcgcg
    14101 ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa attcgccatc aacttgagca
    14161 catcctcaac gaagaacata tcgctcacga gccgcgggcg ttgcaattgc tggcgcgcgc
    14221 cgctgaaggc agcctgcgag atgccttaag tctgaccgac caggcgattg ccagcggtga
    14281 cggccaggtt tcaacccagg cagtcagtgc gatgctgggt acgcttgatg acgatcaggc
    14341 gctgtcgctg gttgaagcga tggtcgaggc caacggcgag cgcgtaatgg cgctgattaa
    14401 tgaagccgct gcccgtggta tcgagtggga agcgttgctg gtggaaatgc tcggcctgtt
    14461 gcatcgtatt gcgatggtac aactttcgcc tgctgcactt ggcaacgaca tggccgccat
    14521 tgagctgcgg atgcgtgaac tggcgcgcac cataccgccg acggatattc agctttacta
    14581 tcagacgctg ttgattggtc gcaaagaatt accgtatgcg ccggaccgcc gcatgggcgt
    14641 tgagatgacg ctgctgcgcg cgctggcgtt ccatccacgt atgcctctgc ctgagccaga
    14701 agtgccgcgc cagtcctttg cacccgtcgc gccaacggca gtaatgacgc caacccaggt
    14761 gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    14821 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    14881 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    14941 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    15001 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    15061 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    15121 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagtca
    15181 actttcgcta ccaaaactgg tcgaacaggt ggccttaaat gcctggaaag aggagagcga
    15241 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcgca
    15301 gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca acggttgaac tgactatcgt
    15361 tgaagatgat aatcccgcgg tgcgtacgcc gctggaatgg cgtcaggcga tatacgaaga
    15421 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    15481 gttcttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    15541 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    15601 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    15661 tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    15721 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    15781 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    15841 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    15901 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    15961 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    16021 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    16081 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    16141 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    16201 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    16261 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    16321 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    16381 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    16441 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tcccttgccg ggcgtcataa
    16501 gattcgtttt taagcaaacg agagcaggat cacctgctct cgcttgaaat tattctccct
    16561 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    16621 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    16681 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    16741 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    16801 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    16861 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    16921 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    16981 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgactgtgc
    17041 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    17101 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    17161 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    17221 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gacggcgaaa
    17281 ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    17341 aaatcaccga tgaagagtac aaagagttct acaaacacat cgcccacgac tttaatgatc
    17401 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    17461 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    17521 acgtccagcg tgtgttcatc atggacgacg cagaacagtt catgccgaac tatctgcgct
    17581 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    17641 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    17701 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    17761 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    17821 tgcgttttgc ttctacccat accgattctt ctgcgcagac cgtatctctg gaagactacg
    17881 tttcccgcat gaaagaaggg caggagaaaa tctactacat caccgcagac agctatgcgg
    17941 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    18001 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    18061 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    18121 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgtgtgaaa gccttgctcg
    18181 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    18241 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    18301 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    18361 cggcagatac tgaagatgaa gcgaagttca gcgagtgggt agaactgctg ctggatcagg
    18421 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    18481 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    18541 catcatctgc actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    18601 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaat tcgacacatt
    18661 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    18721 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    18781 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    18841 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    18901 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    18961 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    19021 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    19081 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    19141 cccgtaaaga cgatcaggaa gaaaccgtac gtaaacgtct ggttgaatac catcagatga
    19201 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    19261 aagttgacgg caccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    19321 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    19381 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    19441 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    19501 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    19561 cgtggttgat acctcacggt tgttatggtg gccattgctg cgcggcgtta ttttgccgct
    19621 gcgctcgccg cgtgtggcga agctgtatgc ctctgtctgg atggaaggtg gctcgccgct
    19681 gatggtttac agccgccagc aacagcaggc gctggcacaa cgtttaccgg agacgcccgt
    19741 agcgctggga atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    19801 agagcatgta gatcatattg tggtgatgcc gctttatccg caattctcct gttctacggt
    19861 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    19921 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    19981 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    20041 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    20101 cgaactggct tccgcactgg ggatggcacc ggaaaaagtg atgatgacct ttcagtcgcg
    20161 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    20221 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    20281 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgccggcg ggaaaaaata
    20341 tgaatatatt ccagcgctta atgccacgcc agaacatatc gaaatgatgg ctaatcttgt
    20401 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    20461 ggcggttttc atcattcgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    20521 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    20581 ccgcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    20641 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    20701 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    20761 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    20821 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    20881 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    20941 ataacaacaa gcagccacaa tttcctctat cgcttgcgga aaacgcgctt caggtgaaag
    21001 ggggtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    21061 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    21121 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    21181 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    21241 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    21301 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    21361 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    21421 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    21481 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    21541 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    21601 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    21661 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    21721 ggtgattgag gatgacgtag ccgaagcgct ttatcaggag ctaaaacaga aaaacctgat
    21781 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    21841 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    21901 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    21961 tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    22021 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    22081 agcctcggca ctggttctca cctcttatct ggtgcgttgc aaaccgggtg aacccatgcc
    22141 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    22201 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    22261 tcacgtctct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    22321 tccgttgttg gcatctgaca aggcgctgga ctgggtagat ctggtgctgt gcaccgccgg
    22381 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    22441 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    22501 ccacaaggat tgccagaatc cgctgcgtgt atattcgcac attgcgccgt acatgggcgg
    22561 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    22621 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    22681 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    22741 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    22801 cctggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    22861 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    22921 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacctcac
    22981 ccgccggtgg cgtttccagc agttccagca tggtacgggc gatttcacgc tcgcccatca
    23041 ctacctgatt cgcaccacgt tcggtgatat acgccacttc atcgtcataa tgggcgcggg
    23101 caataatctc aatatccgga tttttcgcgc gggcagatgc cacaatctca cccgcttcat
    23161 aaccgttggg aatcgtcagg atcagccatt ttgcacattc cagatgcgcc agttgcataa
    23221 tttcttcgtt cgccgcattg cccaatactg cgcggacccc gcgctctcgc agctcatcaa
    23281 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    23341 ccagcaggct gcctacacga ccgtaaccca ccagtagcgc atggttgcaa atatccactg
    23401 ggatctgctt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    23461 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    23521 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    23581 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    23641 tacgttggga gtgaccaaac agtcgcacca ggaaaaatgc ggctaacgac ttaccaaaca
    23701 gaataatcgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    23761 acaacatccc gacggagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcagcgtat
    23821 cgtgggcggc acggtgactc agttcagact cgttcagtac catcccggca aagaacgcac
    23881 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    23941 ccagcaccga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    24001 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc actttgccga
    24061 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    24121 ctgccactgc gggcagcaac accagcgtca gaaccattac caggtcttcc acaatcaacc
    24181 aaccgatggc gatttgccca cgctgactgt caattaattg ccgttcttca agtgcgcgca
    24241 gtaacaccac ggtactggcg gtggaaagac ataaaccgaa cacgataccg gtcattaacg
    24301 accagcccag cacggcagag agcgccatac ccagcagcgt cgccacggct atctgggcga
    24361 tcgcaccggg aatggcgatg gcctttaccg ccatcaaatc cttcagcgaa aagtgcaaac
    24421 cgacgccaaa catcaacaga atgacgccca gttcagccag ttccggcgca agcttggtat
    24481 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    24541 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    24601 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    24661 attgtccatt tttggccggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    24721 gatgatgttc atgaataatt attgaatttt gcagaaaaat ggaatttgct gcaaaaaaag
    24781 catggatcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    24841 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    24901 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    24961 ttctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccaaaag
    25021 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    25081 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    25141 ccgtccagtg caggctggcg tagggtaaaa tcagcgtaaa cggctcaacg ccgaggatag
    25201 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    25261 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    25321 cgaatttttg catcagataa aaggtgtaat agct
//
LOCUS       DAJTMK010000043        25226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTMK010000043 DAJTMK010000000
VERSION     DAJTMK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25226
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.044"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(60..485)
                     /locus_tag="OO135_001505"
     CDS             complement(60..485)
                     /locus_tag="OO135_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5397407.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKKPAAFWVMPIS"
     gene            864..1175
                     /gene="rpsJ"
                     /locus_tag="OO135_001506"
     CDS             864..1175
                     /gene="rpsJ"
                     /locus_tag="OO135_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5397408.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1208..1837
                     /gene="rplC"
                     /locus_tag="OO135_001507"
     CDS             1208..1837
                     /gene="rplC"
                     /locus_tag="OO135_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5397409.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1848..2453
                     /gene="rplD"
                     /locus_tag="OO135_001508"
     CDS             1848..2453
                     /gene="rplD"
                     /locus_tag="OO135_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5397410.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2450..2752
                     /gene="rplW"
                     /locus_tag="OO135_001509"
     CDS             2450..2752
                     /gene="rplW"
                     /locus_tag="OO135_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5397411.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2770..3591
                     /gene="rplB"
                     /locus_tag="OO135_001510"
     CDS             2770..3591
                     /gene="rplB"
                     /locus_tag="OO135_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5397412.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3608..3886
                     /gene="rpsS"
                     /locus_tag="OO135_001511"
     CDS             3608..3886
                     /gene="rpsS"
                     /locus_tag="OO135_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5397413.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3901..4233
                     /gene="rplV"
                     /locus_tag="OO135_001512"
     CDS             3901..4233
                     /gene="rplV"
                     /locus_tag="OO135_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5397414.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4251..4952
                     /gene="rpsC"
                     /locus_tag="OO135_001513"
     CDS             4251..4952
                     /gene="rpsC"
                     /locus_tag="OO135_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5397415.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4965..5375
                     /gene="rplP"
                     /locus_tag="OO135_001514"
     CDS             4965..5375
                     /gene="rplP"
                     /locus_tag="OO135_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5397416.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5375..5566
                     /gene="rpmC"
                     /locus_tag="OO135_001515"
     CDS             5375..5566
                     /gene="rpmC"
                     /locus_tag="OO135_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5397417.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5566..5820
                     /gene="rpsQ"
                     /locus_tag="OO135_001516"
     CDS             5566..5820
                     /gene="rpsQ"
                     /locus_tag="OO135_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5397418.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5985..6356
                     /gene="rplN"
                     /locus_tag="OO135_001517"
     CDS             5985..6356
                     /gene="rplN"
                     /locus_tag="OO135_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5397419.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6367..6681
                     /gene="rplX"
                     /locus_tag="OO135_001518"
     CDS             6367..6681
                     /gene="rplX"
                     /locus_tag="OO135_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5397420.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6696..7235
                     /gene="rplE"
                     /locus_tag="OO135_001519"
     CDS             6696..7235
                     /gene="rplE"
                     /locus_tag="OO135_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5397421.1"
                     /translation="MAKLHDYYKDEVVKKFMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7250..7555
                     /gene="rpsN"
                     /locus_tag="OO135_001520"
     CDS             7250..7555
                     /gene="rpsN"
                     /locus_tag="OO135_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5397422.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7589..7981
                     /gene="rpsH"
                     /locus_tag="OO135_001521"
     CDS             7589..7981
                     /gene="rpsH"
                     /locus_tag="OO135_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5397423.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7994..8527
                     /gene="rplF"
                     /locus_tag="OO135_001522"
     CDS             7994..8527
                     /gene="rplF"
                     /locus_tag="OO135_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5397424.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8537..8890
                     /gene="rplR"
                     /locus_tag="OO135_001523"
     CDS             8537..8890
                     /gene="rplR"
                     /locus_tag="OO135_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5397425.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8905..9408
                     /gene="rpsE"
                     /locus_tag="OO135_001524"
     CDS             8905..9408
                     /gene="rpsE"
                     /locus_tag="OO135_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5397426.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9412..9591
                     /gene="rpmD"
                     /locus_tag="OO135_001525"
     CDS             9412..9591
                     /gene="rpmD"
                     /locus_tag="OO135_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5397427.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9595..10029
                     /gene="rplO"
                     /locus_tag="OO135_001526"
     CDS             9595..10029
                     /gene="rplO"
                     /locus_tag="OO135_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5397428.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10037..11368
                     /gene="secY"
                     /locus_tag="OO135_001527"
     CDS             10037..11368
                     /gene="secY"
                     /locus_tag="OO135_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5397429.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11400..11516
                     /gene="rpmJ"
                     /locus_tag="OO135_001528"
     CDS             11400..11516
                     /gene="rpmJ"
                     /locus_tag="OO135_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5397430.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11663..12019
                     /gene="rpsM"
                     /locus_tag="OO135_001529"
     CDS             11663..12019
                     /gene="rpsM"
                     /locus_tag="OO135_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5397431.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12036..12425
                     /gene="rpsK"
                     /locus_tag="OO135_001530"
     CDS             12036..12425
                     /gene="rpsK"
                     /locus_tag="OO135_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5397432.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12459..13079
                     /gene="rpsD"
                     /locus_tag="OO135_001531"
     CDS             12459..13079
                     /gene="rpsD"
                     /locus_tag="OO135_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5397433.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRIVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13105..14094
                     /gene="rpoA"
                     /locus_tag="OO135_001532"
     CDS             13105..14094
                     /gene="rpoA"
                     /locus_tag="OO135_001532"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5397434.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14135..14518
                     /gene="rplQ"
                     /locus_tag="OO135_001533"
     CDS             14135..14518
                     /gene="rplQ"
                     /locus_tag="OO135_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5397435.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14625..14993
                     /locus_tag="OO135_001534"
     CDS             14625..14993
                     /locus_tag="OO135_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR5397436.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGKPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15004..15429
                     /gene="zntR"
                     /locus_tag="OO135_001535"
     CDS             15004..15429
                     /gene="zntR"
                     /locus_tag="OO135_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR5397437.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15485..15703
                     /gene="arfA"
                     /locus_tag="OO135_001536"
     CDS             15485..15703
                     /gene="arfA"
                     /locus_tag="OO135_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR5397438.1"
                     /translation="MSRYQHIKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15700..16110)
                     /gene="mscL"
                     /locus_tag="OO135_001537"
     CDS             complement(15700..16110)
                     /gene="mscL"
                     /locus_tag="OO135_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR5397439.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16240..17616)
                     /gene="trkA"
                     /locus_tag="OO135_001538"
     CDS             complement(16240..17616)
                     /gene="trkA"
                     /locus_tag="OO135_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5397440.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17638..18927)
                     /gene="rsmB"
                     /locus_tag="OO135_001539"
     CDS             complement(17638..18927)
                     /gene="rsmB"
                     /locus_tag="OO135_001539"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5397441.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18973..19920)
                     /gene="fmt"
                     /locus_tag="OO135_001540"
     CDS             complement(18973..19920)
                     /gene="fmt"
                     /locus_tag="OO135_001540"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5397442.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19935..20444)
                     /gene="def"
                     /locus_tag="OO135_001541"
     CDS             complement(19935..20444)
                     /gene="def"
                     /locus_tag="OO135_001541"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5397443.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20574..21698
                     /gene="dprA"
                     /locus_tag="OO135_001542"
     CDS             20574..21698
                     /gene="dprA"
                     /locus_tag="OO135_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5397444.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21670..22143
                     /locus_tag="OO135_001543"
     CDS             21670..22143
                     /locus_tag="OO135_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5397445.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22172..22714
                     /locus_tag="OO135_001544"
     CDS             22172..22714
                     /locus_tag="OO135_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR5397446.1"
                     /translation="MAKSALFTVRNNESCSKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22719..23291
                     /gene="tsaC"
                     /locus_tag="OO135_001545"
     CDS             22719..23291
                     /gene="tsaC"
                     /locus_tag="OO135_001545"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5397447.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23296..24114
                     /gene="aroE"
                     /locus_tag="OO135_001546"
     CDS             23296..24114
                     /gene="aroE"
                     /locus_tag="OO135_001546"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5397448.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLNCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24111..24368
                     /locus_tag="OO135_001547"
     CDS             24111..24368
                     /locus_tag="OO135_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5397449.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24344..24898)
                     /locus_tag="OO135_001548"
     CDS             complement(24344..24898)
                     /locus_tag="OO135_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5397450.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6448 a         6003 c         6565 g         6210 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattaccca aaaggcagcg ggttttttta atgattcttt tcctctcatg
      121 agcagaccaa taactacggc tccgcaagcg aatgaggcag caaggaaaac cagtcgtggt
      181 aagaaggccc atgaatgcca ggcgccaagg gcggcgagaa acttcacatc accatagcct
      241 aatccttctt tatgtcgcag tatgcgatac ccccagtaaa tgacagcgaa tgtgccgtaa
      301 ccaacaattg tcccccataa cgcatcggct aaaccatcag gatgacaaac ttgatagaac
      361 aaaagacctg accagagtaa cgggcacgta aatctgtcag gtaacaagcc atgctttgca
      421 tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa tcagaaaggg aagggtagtg
      481 tccataaaac atcctctgta attggagtat caccatcgaa taactccgga tgctcagcaa
      541 cagagagagc gctgatttgt taacctgatt ccggtgaaaa acgctatctt cacaacctta
      601 caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga ttaatttcta cccgatgctt
      661 gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg tagttgacag caggttcaat
      721 ctgaacctca gttagccgaa tttggctacc taacaatgct cccaatcggg gagctacgta
      781 agaacggtta cactctccca tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat
      841 aaaataattg gagctctggt ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt
      901 ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt cgagactgcc aagcgcactg
      961 gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc actgttctga
     1021 tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact cacttgcgtc
     1081 tggttgacat cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg
     1141 ctgccggtgt agacgtgcag atcagcctgg gttaatcagg tcattgagcg attgagaggt
     1201 tgaaacaatg attggtttag tcggtaaaaa agtgggtatg acccgtatct tcacagaaga
     1261 cggcgtttct atcccagtaa ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa
     1321 agacctggct aacgatggct accgtgctat tcaggtgacc accggtgcta aaaaagctaa
     1381 ccgtgtgacc aagcctgaag ctggccactt cgctaaagct ggcgtagaag ctggccgtgg
     1441 tctgtgggaa ttccgcctgg ctgaaggcga agagttcact gtaggtcaga gcattagcgt
     1501 tgaactgttt gctgacgtta aaaaagttga cgtaactggc acctctaaag gcaaaggttt
     1561 cgcaggtacc gttaagcgct ggaacttccg tacccaggac gctactcacg gtaactcctt
     1621 gtctcaccgc gttccgggtt ctatcggtca gaaccagact ccgggcaaag tgttcaaagg
     1681 caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt
     1741 acgcgttgac gctgagcgca acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg
     1801 tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg gaattagtat
     1861 tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt gatttcaacg
     1921 aagcgctggt tcaccaagtt gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg
     1981 ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca
     2041 ccggccgtgc gcgttctggt tctatcaaga gcccgatctg gcgttctggt ggcgtgacct
     2101 ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc
     2161 tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc gagaagttct
     2221 ctgtagaagc gccgaaaact aagctgctgg cacagaaact gaaagacatg gctctggaag
     2281 atgtgctgat catcaccggt gagctggacg aaaacctgtt cctggctgcg cgcaacctgc
     2341 acaaggttga cgtacgcgat gcaactggta tcgacccggt tagcctgatc gccttcgaca
     2401 aagtcgtaat gactgctgat gctgttaagc aagttgagga gatgctggca tgattcgtga
     2461 agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat
     2521 ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa
     2581 agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa
     2641 agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga aaaaagctta
     2701 cgtcaccctg aaagaaggcc agaatctgga cttcgttggc ggcgctgagt aagtcggagg
     2761 agtaacacaa tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt
     2821 aaagtggtta accctgagct gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac
     2881 agcaaatccg gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat cggtggtggc
     2941 cacaagcagg cttaccgtat tgttgacttc aaacgcaaca aagacggtat cccggcagtt
     3001 gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa
     3061 gacggtgaac gccgttacat cctggcccct aaaggcctga aagctggcga ccagattcag
     3121 tctggcgttg atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa catcccggtt
     3181 ggttctactg ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc
     3241 gctggtactt acgttcagat cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt
     3301 tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga agttggcaat
     3361 gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt
     3421 ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc cacatggtgg tggtgaaggt
     3481 cgtaactttg gtaagcaccc ggtaactccg tggggcgttc agaccaaagg taagaagacc
     3541 cgcagcaaca agcgtactga taaattcatc gtacgtcgcc gtagcaaata attttagagg
     3601 ataagccatg ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa
     3661 ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc
     3721 aacgatcttt cctaacatga tcggtttgac catcgctgtc cataatggtc gtcagcacgt
     3781 tccggtattt gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg caccgactcg
     3841 tacttatcgc ggccacgctg ctgataaaaa agcgaagaag aaataaggta ggaggaagag
     3901 atggaaacta tcgctaaaca tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt
     3961 gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg atattttgac ctacaccaac
     4021 aagaaagcgg ctgtactggt caagaaagtt ctggaatctg ccattgctaa cgctgaacac
     4081 aacgatggcg ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg
     4141 agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc
     4201 agccacatca ctgtggttgt gtccgatcgc tgagactctg gagactagca atgggtcaga
     4261 aagtacatcc taatggtatt cgcctgggta ttgtaaaacc atggaactct acctggtttg
     4321 cgaacaccaa agaattcgct gacaacctgg acagcgattt taaagtacgt cagtacctga
     4381 ctaaggaact ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg gctaagagca
     4441 tccgtgtaac cattcacact gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg
     4501 tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag atcaacatcg
     4561 ccgaagttcg taagcctgaa ctggacgcaa aactggttgc tgacagcatc acttctcagc
     4621 tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc
     4681 tgggcgctaa aggtattaaa gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac
     4741 gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact
     4801 acaacacctc tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg tggatcttca
     4861 aaggcgagat cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc
     4921 ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc
     4981 gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg
     5041 ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga
     5101 tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag atctggatcc
     5161 gtgtgttccc ggacaaaccg atcactgaaa agccgctggc agtgcgtatg ggtaaaggta
     5221 aaggtaacgt ggagtattgg gttgccttga ttcagccggg taaagtcctg tatgaaatgg
     5281 acggtgttcc ggaagagctg gcccgtgaag cattcaagct ggcagcagcg aaactgccga
     5341 ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa
     5401 gagcgttgaa gagctgaaca ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg
     5461 tatgcaggct gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga
     5521 tgtcgcacgc gttaagactt tactgaacga gaaggcgggt gcgtaatgac cgataaaatc
     5581 cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga aatccattgt tgttgctatc
     5641 gaacgttttg tgaaacaccc gatctacggt aaattcatca agcgtacgac caaactgcac
     5701 gtacatgacg agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt
     5761 ccgctgtcca agactaaatc ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa
     5821 tacagtacac tctctcgata cgaataaacg gctcagaaat gagccgttta ttttttctac
     5881 ccatatcctt gaagcggtgt tataatgccg cgccctcgat atggggattt ttaacgacct
     5941 gattttcggg tctcagtagt agttgacatt agcggagcac taaaatgatc caagaacaga
     6001 ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg
     6061 gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat caagatcacc atcaaagaag
     6121 caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca
     6181 agaagggtgt tcgtcgcccg gacggttctg tcattcgctt cgatggtaat gcttgtgttc
     6241 ttctgaacaa caacagcgag cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg
     6301 agcttcgtag tgagaagttc atgaaaatta tctctctggc accagaagta ctctaaggag
     6361 cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat
     6421 aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt
     6481 atcaacctgg ttaagaaaca tcagaagccg gttccggccc tgaaccaacc gggtggcatc
     6541 gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc
     6601 aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa aagtccgttt cttcaagtct
     6661 aacagcgaaa ctatcaagta atttggagta gtacgatggc gaaactgcat gattactaca
     6721 aagacgaagt agttaaaaaa ttcatgactg agtttaacta caattctgtc atgcaagtcc
     6781 ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac
     6841 tgctggataa cgcagcagca gacctggcag caatctccgg tcaaaaaccg ctgatcacca
     6901 aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag
     6961 taactctgcg tggcgaacgc atgtgggagt tctttgagcg cctgatcact attgctgtac
     7021 ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca
     7081 gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg
     7141 ttcgtggttt ggatattacc attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc
     7201 tgctggctgc ctttgacttc ccgttccgca agtaaggtag ggttactaaa tggctaagca
     7261 atcaatgaaa gcacgcgaag taaaacgcgt agctttagct gataaatact tcgcgaaacg
     7321 cgctgaactg aaagcgatca tctctgatgt gaacgcttcc gacgaagatc gttggaacgc
     7381 tgttctcaag ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg
     7441 ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc gggttgagcc gtattaaggt
     7501 ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc
     7561 accaattgaa tcacgggagt aaagacagat gagcatgcaa gatccgatcg cggatatgct
     7621 gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa
     7681 gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag attttaaagt
     7741 tgaaggcgac accaagcctg aactggaact tactctgaag tatttccagg gcaaagctgt
     7801 tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc atctataaac gtaaagatga
     7861 gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt tctacctcta aaggtgttat
     7921 gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa attatctgct acgtagccta
     7981 atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc gttgttcctg ccggcgttga
     8041 cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa aacggcgagc tgactcgtac
     8101 tctcaacgat gctgttgaag ttaaacatgc agataatacc ctgaccttcg gtccgcgtga
     8161 tggttacgca gacggttggg cacaggctgg taccgcgcgt gccctgctga actcaatggt
     8221 tatcggtgtt accgaaggct tcactaagaa gctgcagctg gttggtgtag gttaccgtgc
     8281 agcggttaaa ggtaatgtga ttaacctgtc tctgggtttc tctcatcctg ttgaccatca
     8341 gctgcctgcg ggtatcactg ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgc
     8401 tgataagcag gtgatcggcc aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc
     8461 ttataaaggc aagggtgttc gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa
     8521 gaagtaaggt aacactatgg ataagaaatc tgctcgtatc cgtcgtgcga cccgcgcacg
     8581 ccgcaagctc caggagctgg gcgcaactcg cctggtggta catcgtaccc cgcgtcacat
     8641 ttacgcacag gtaattgcac cgaacggttc tgaagttctg gtagctgctt ctactgtaga
     8701 aaaagctatc gctgaacaac tgaagtacac cggtaacaaa gacgcggctg cagctgtggg
     8761 taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc
     8821 cgggttccaa tatcatggtc gtgtccaggc actggcagat gctgcccgtg aagctggcct
     8881 tcagttctaa ggtagaggtg taagatggct cacatcgaaa aacaagctgg cgaactgcag
     8941 gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc
     9001 ttcacagctc tgactgtagt tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa
     9061 gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa aagcccgtcg caatatgatt
     9121 aacgtcgcgc tgaataacgg cactctgcaa caccctgtta aaggtgttca cacgggttct
     9181 cgcgtattca tgcagccggc ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc
     9241 gccgttctgg aagtcgctgg ggttcataac gttctggcta aagcatatgg ttccaccaac
     9301 ccgatcaacg tggttcgtgc aactattgat ggcctggaaa atatgaattc tccagaaatg
     9361 gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg ggaaataaac catggcaaag
     9421 actattaaaa ttactcaaac ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg
     9481 ctgcttggcc tgggtctgcg tcgtattggt cacaccgtag agcgcgagga tactcctgct
     9541 attcgcggta tgatcaacgc ggtttccttc atggttaaag ttgaggagta agagatgcgt
     9601 ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt
     9661 atcggttctg gcctcggtaa aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct
     9721 ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg
     9781 aaattcggct tcacttctcg taaagcagcg attacagccg aagttcgtct gtctgacctg
     9841 gctaaagtag aaggcggtgt tgtagacctg aacacgctga aagcggctaa cattatcggt
     9901 atccagatcg agttcgcgaa agtgatcctg gctggcgaag taacgactcc ggtaactgtt
     9961 cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc
    10021 gaggaataag tagcagatgg ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg
    10081 cttaggcgag ctgaaacgca gactgctgtt tgttatcggt gcgctgattg tgttccgtat
    10141 tggctctttt attccgatcc ctggtattga tgccgctgta cttgccaaac tgcttgagca
    10201 acagcgaggc accatcattg agatgtttaa catgttctct ggtggtgctc tcagccgtgc
    10261 ttctatcttt gctctgggga tcatgccgta tatttcggcg tcgatcatta tccagctgct
    10321 gacggtggtt cacccaacgt tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa
    10381 gatcagccag tacacccgct acggtactct ggtgctggca atattccagt cgatcggtat
    10441 tgctaccggt ctgccgaata tgcctggtat gcaaggcctg gtgattaacc cgggctttgc
    10501 attctacttc accgctgttg taagtctggt cacaggaacg atgttcctga tgtggttggg
    10561 cgaacagatc actgaacgag gtatcggcaa cggtatttca atcattatct tcgccggtat
    10621 tgtcgcggga ctcccgccag ccattgccca tactatcgag caagcgcgtc aaggcgacct
    10681 gcacttcctc gtgttgctgt tggttgcagt attagtattt gcagtgacgt tctttgttgt
    10741 atttgttgag cgtggtcaac gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg
    10801 tcgtgtctat gctgcacaga gcacacattt accgctgaaa gtgaatatgg cgggggtaat
    10861 cccggcaatc ttcgcttcca gtattattct gttcccggcg accatcgcgt catggttcgg
    10921 gggcggtact ggttggaact ggctgacaac aatttcgctg tatttgcagc ctgggcaacc
    10981 gctttatgtg ttactctatg cgtctgcaat catcttcttc tgtttcttct acacggcgtt
    11041 ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag tccggtgcat ttgtaccagg
    11101 aattcgtccg ggagagcaaa cggcgaagta tatcgataaa gtaatgaccc gcctgaccct
    11161 ggttggtgcg ctgtatatta cctttatctg cctgatcccg gagttcatgc gtgatgcaat
    11221 gaaagtaccg ttctacttcg gtgggacctc actgcttatc gttgttgtcg tgattatgga
    11281 ctttatggct caagtgcaaa ctctgatgat gtccagtcag tatgagtctg cattgaagaa
    11341 ggcgaacctg aaaggctacg gccgataatt ggtcgcccga gaagttacgg agagtaaaaa
    11401 tgaaagttcg tgcttccgtc aagaaattat gccgtaactg caaaatcgtt aagcgtgatg
    11461 gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa acagcgccaa ggctgatttt
    11521 ttcgcatatt tttcttgcaa agttgggttg agctggctag attagccagc caatcttttg
    11581 tatgtttgtg cgtttccatt tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca
    11641 tattaaatag taggagtgca tagtggcccg tatagcaggc attaacattc ctgatcataa
    11701 gcatgccgta atcgcattaa cttcgattta tggcgtcggc aagacccgtt ctaaagccat
    11761 cctggctgca gcgggtatcg ctgaagatgt taagatcagt gagctgtctg aaggacaaat
    11821 cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat
    11881 cagcatgagc atcaagcgcc tgatggatct tggttgctat cgcggtttgc gtcatcgtcg
    11941 tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca cgtacccgta agggtccgcg
    12001 caaaccgatc aagaaataat cggggtgatt gaataatggc aaaggcacca attcgtgcac
    12061 gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca tatccatgct tctttcaaca
    12121 acaccatcgt gactatcact gatcgtcagg gtaacgcgtt gggttgggca acagccggtg
    12181 gttccggttt ccgtggttct cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc
    12241 gttgcgctga cgccgtgaaa gaatacggca tcaagaatct ggaagttatg gttaaaggtc
    12301 cgggtccagg ccgcgaatct actattcgtg ctctgaacgc cgcaggtttc cgcatcacta
    12361 acattactga tgtgactccg atccctcata acggttgtcg tccgccgaaa aaacgtcgcg
    12421 tataacgcct cgttttccag gtttgttgga gaaagaaaat ggcaagatat ttgggtccta
    12481 agctcaagct gagccgtcgt gagggcaccg acttattcct taagtctggc gttcgcgcga
    12541 tcgataccaa gtgtaaaatt gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc
    12601 tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc
    12661 tggagcgtca gttccgtaac tactacaaag aagcagcacg tctgaaaggc aacaccggtg
    12721 aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg
    12781 gtgccactcg tgcagaagca cgtcagctgg ttagccataa agcaattatg gtaaacggtc
    12841 gtattgttaa catcgcttct tatcaggtta gtccgaatga cgttgtaagc attcgtgaga
    12901 aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc
    12961 caacttggct ggaagttgat gctggcaaga tggaaggtac gtttaagcgt aagccggagc
    13021 gttctgatct gtctgcggac attaacgaac acctgatcgt cgagctttac tccaagtaaa
    13081 gcttagtacc aaagagagga cacaatgcag ggttctgtga cagagtttct aaaaccgcgc
    13141 ctggttgata tcgagcaagt gagttcgacg cacgccaagg tgacccttga gcctttagag
    13201 cgtggctttg gccatactct gggtaacgca ctgcgccgta ttctgctctc atcgatgccg
    13261 ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac atgagtacag caccaaagaa
    13321 ggcgttcagg aagatatcct ggaaatcctg ctcaacctga aagggctggc ggtgagagtt
    13381 cagggcaaag atgaagttat tcttaccttg aataaatctg gcattggccc tgtgactgca
    13441 gccgatatca cccacgacgg tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac
    13501 ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat
    13561 gtgccggctt ctacccgaat tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg
    13621 gtcgacgcat gctacagccc tgtggagcgt attgcctaca atgttgaagc agcgcgtgta
    13681 gaacagcgta ccgacctgga caagctggtc atcgaaatgg aaaccaacgg cacaatcgat
    13741 cctgaagagg cgattcgtcg tgcggcaacc attctggctg aacaactgga agctttcgtt
    13801 gacttacgtg atgtacgtca gcctgaagtg aaagaagaga aaccagagtt cgatccgatc
    13861 ctgctgcgcc ctgttgacga tctggaattg actgtccgct ctgctaactg ccttaaagca
    13921 gaagctatcc actatatcgg tgatctggta caacgtaccg aggttgagct ccttaaaacg
    13981 cctaaccttg gtaaaaaatc tcttactgag attaaagacg tgctggcttc ccgtggactg
    14041 tctctgggca tgcgcctgga aaactggcca ccggcaagca tcgctgacga gtaaccggat
    14101 cacaggttaa ggttttactg agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca
    14161 actgaaccgc aacagcagcc atcgccaggc tatgttccgc aatatggcag gttcactggt
    14221 tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga
    14281 gccgctgatt actcttgcca agactgatag cgttgctaat cgtcgtctgg cattcgcccg
    14341 tactcgtgat aacgagatcg tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag
    14401 ccgtgccggt ggttacactc gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc
    14461 tatggcttac atcgagctgg ttgatcgttc agagaaagca gaagctgctg cagagtaatc
    14521 tgaagcaacg taaaaaaacc cgccccggcg ggttttttta tacccgtagc atccccactt
    14581 atctacaata gctgtactct ttttgttcat cccctggagt atttatgtgg ttacttgacc
    14641 agtgggcaga gcgtcatata gcagaagcgc aagcgaaagg tgagtttgat aacctggcag
    14701 gtagcggcaa accattgata ctggatgatg attctcatgt gccaccggaa ttacgtgcgg
    14761 ggtatcgctt gctgaagaat gccggttgct taccgccaga acttgagcag cggagggaag
    14821 caattcagct tctggatatc ctgagaggta tccgtcacga tgatccgcaa tatcaagagg
    14881 ttagccgtcg attgtcatta ctggaattga agctgcgaca agctggattg agtaccgatt
    14941 ttttacgcgg cgattatgct gacaagttgt tggacaaaat caacgataac tagtggagta
    15001 tgtatgtatc gcattggtga gctggcaaaa atggcggaag taacacctga cacgattcgt
    15061 tattacgaaa aacagcagat gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg
    15121 tataccgaaa gcgatctcca gcgattgaaa tttatccggc atgccagaca actaggtttc
    15181 agtctggagt cgatccgcga gttgctgtcg atccgcatcg atcctgaaca ccatacctgt
    15241 caggagtcaa aaggcattgt gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag
    15301 ttgcagagta tgcagcgttc cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat
    15361 agcagtgttt attgttcgat tcttgaagct cttgaacaag gggcgagtgg cgttaagagt
    15421 ggctgttgat tttttgcact ggcaggatta cactcgcgcc gtaaaataac caattggagt
    15481 ttttatgagc cgatatcagc atattaaagg gcagataagg gataatgcga tagaagcatt
    15541 actacatgat cccttattcc gacagcgcgt agagaaaaat aagaagggga aaggcagtta
    15601 catgcgaaaa ggaaaacatg gcaatcgggg gaactgggag gccagtggca agaaagtaaa
    15661 tcactttttt accactggtc ttctgctttc aggcgcttgt taagagcggt tattctgctc
    15721 tttcagcaaa tcacgaattt ctgccagtaa tacttcttct ttagttggtg caggtgcggc
    15781 agctggttct tcttttttcc gattcagttt gttgattagc ttaatcgcca taaagatggc
    15841 aaaggccaca atcagaaaat caaagacgtt ttgaatgaag acaccgtaat gcatcacaac
    15901 agcagggata tccccctgcg catcgcgtag cgtgacagca aactgtttaa agtcgatccc
    15961 gccaattaat aagcccagag gtggcatgat gatatcggca accagtgaag atacaatctt
    16021 cccgaatgcc gcaccgataa tgacacccac cgccaaatcc accacgttcc cgcgcatcgc
    16081 aaattcgcga aattctttaa taatgctcat gttattctcc ctatgaagcc gacaaccata
    16141 agtctaacaa atgttaagcc attttcctgc caggaatgaa tttaattaat ccttgataat
    16201 caaagggaaa taataaggcg tcattagacg ccttattaat tacaagaaga aagggcttgg
    16261 ctggaagagt ctttcgacgt cggtaataaa ctttttatct gtgaggaaca taattacgtg
    16321 atcgccttgc tcaatgcgca gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac
    16381 cgctccaata atcgttcctg gcggtagctt gatttcgtca atgactctgc cgacaacgcg
    16441 tgaggtgctt tcatcaccgt gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa
    16501 tgaggaaaca ccaacaatat ctgcttttcg cacatggcta agcaacgcag aaatagttgc
    16561 ttgttgtggt gaaattgcaa tatcgatcac gctcccctga accagatcca cataagcgcg
    16621 acgctggatc aataccatca cctttttcgc acccatacgt ttggcaagca tggcggacat
    16681 gatattggcc tcgtcatcgt tggtgacggc aataaacaga tcaacttgat cgatatgttc
    16741 ttcggccagc agttcttgat ccgacgcatc accaaaaaag acgatcgtat tctgcaactt
    16801 ttccgccagt tcggcagcgc gctgctgatt acgttcgatg agtttaacgc tgtaatcttt
    16861 ttccagacga cgcgccagcc ctgcaccgat attaccgcca ccaaccagca tgatccgctt
    16921 atacggtttt tccagtcgct gtaattcact catcaccgcg cggatatgct gtgaagcggc
    16981 aataaagaac acttcatcac cagcttcaac aatggtcgaa ccttgtggac gaatggggcg
    17041 atcgtggcgg aaaattgctg ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt
    17101 cgaaagtgca ttaccaatca gcgggccgcc ataataggct ttaactacag cgaggctgac
    17161 tttaccctca gcgaagttca ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat
    17221 attatcgata accaactgct ctggtgcgat cagatgatca atcggtacag catctgaatg
    17281 aaatagctta tcggcatcgc gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg
    17341 ggtgttgaaa agtgagtagg ctacctggca ggcaaccata ttggtttcat ctgaactggt
    17401 tacagcaacc agcatatcgg cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga
    17461 gccatgcccc tgcacgactc gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc
    17521 gttggtatcg acaacagtaa tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc
    17581 gccaacctgg ccggcaccca gaatgataat tttcatcagt ctcgacccgt tatctcatca
    17641 ctttttgatt agcttagcgt aaaagaagcc gtcgccctct tcggcaccag gcagattttg
    17701 tttacccggt tgctctggtg ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa
    17761 aaaagcttta atctgcaggc tattctcttc cggtaacacc gaacaggtgg catagaccag
    17821 ggttccaccg gattttaaat gcggccaaat ggcgtcgaga atttcagact gcaattgcgc
    17881 gagttccggg atatcgcgat cgcgacgtaa ccatttaata tccggatggc gacgaatcac
    17941 ccccgttgct gaacaaggcg catctaataa aatgcgatca aactgttgct cgccacacca
    18001 ttgggaaggg taacggccat caccttgttt cacggttgcc ttcataccaa ggcgttttaa
    18061 attgtcgtaa acgcgagaga ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc
    18121 tggtgccacc tcaaggatat gcgttgtttt accgccgggg gcggcacaaa gatccagaat
    18181 gtgttcaccg ttttgtggcg caagccaggt catgcaacct tgtgctgatg catcctgaac
    18241 ggtaacccat ccgtcttcaa aaccaggtag cgcatgaaca ggtgcaggtg tttccagacg
    18301 tacagcatca gggtaatccg catgcgggaa acctttcatt cctgcttcat ccagcaatgc
    18361 aagccagctg tcgcgggaat gatgcgtacg attaacgcgc agccacattg gcggacgctg
    18421 gttattggct tcgacgatgg attgccactg ctctggatac gctttttgca gacgcttcag
    18481 caaccaggaa ggatgcagat aacgtgcatc actggcatta aacttggcta ataactcttc
    18541 ttgctgacgc tggaactggc gtaatacacc gttaatcaac cctttaagtt gcggacgctt
    18601 aattgcgata gcgccttcaa ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata
    18661 aagcagttga tacaaaccaa ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat
    18721 cggacgggcc attaacttat taatcagcca gtctaactgc gaaagcgtac gcagtacgcc
    18781 aaagcacaac tcttgaagaa gtgctttgtc tttatcagaa actttttgct ggagcggtgg
    18841 cagaatgttg cttaatgatt gcccttgctc gacgacttgt tcaacggcct gggccgccat
    18901 gctacgtaaa ttacgttgtt ttttcataag tataaaaatg cccggtaaga ccgggcttag
    18961 aagagtggac tatcaggcca gacggttgcc cggaacaaac cattcccgac gagaattcag
    19021 gaggtcttgc gcgctcatcg ctttcttacc cgcaggttgt aacgagagca gattcaggat
    19081 tccatcacca gttgcaacct gaatgccttg tttgttggct tcaaggatcg ttcctggtgc
    19141 agcgttggtt gccgtatcaa tgaccgacgc tttccagact ttaaccggct gtctttcaat
    19201 ttccagccag ctcattggcc atggattgaa agcgcgaatg cagcgttcaa gctgtgctgc
    19261 cgaaagtgac cagtcaatac gcgcttcttc tttactcaac ttttcggcgt aagtgacaag
    19321 agtttcgtcc tgaacttctg gtttcgccgt gccgtctgcc agttgtttca acgtggtgat
    19381 aagcccttgt gggccaagct ctgccagctt gtcgtacagc gtaccactgg tatcttctgc
    19441 agtaatcggg caggagagct tatagagcat gtcaccggtg tctaaaccga catccatttg
    19501 cataatggtc acaccagttt ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc
    19561 accgcgccag cgtggcagca gtgaaccatg aacgttgata cagccaagac gcggcatctc
    19621 cagcactgct ttaggcagaa ttaagccata ggcgacgacg accataacat cagcctgcag
    19681 atcggcgacc agttgctggt tttcttgtgg acgcagggaa acaggttgaa aaacgggcag
    19741 acctttttcc tcagccagaa ctttaaccgg gctgggcatc agttttttac cgcgtcctgc
    19801 cggtcggtct ggctgggtga acacgccaac gacgttatga ccagaagaca acagcgcgtc
    19861 gagatgacgc gctgcaaagt caggtgtacc cgcaaaaata atacgtagtg attctgacac
    19921 gttagttctt atccttaagc ccgggctttc agacgatcca gtttttcaac tttctgacga
    19981 atacgttgtt gtttcaacgg tgacagataa tccataaaca gtttgccgac caggtgatcc
    20041 atctcatgct gaatacagat ggctaacaga ccgtctgctt ccaattcaaa tggtttaccg
    20101 tcgcggtcca gggcgcgaat tttaactttc tctgcgcgtg gcactaaagc acgttgttca
    20161 gggatcgaca ggcaaccttc ttcaatgcct gtttcgccgc ttttttctaa aagctccgga
    20221 ttgattaaca ccagccgttc gtcacggttt tccgaaacat caataacaat gatacgttga
    20281 tggatatcaa cctgggttgc cgccaggcca ataccttctt ctgcgtacat cgtctcgaac
    20341 atatcatcga cgatacgctg aatttctgca ttcacttctt ctaccggttt agcaactttg
    20401 cgaagccgct cgtccggaat atgtaacact tgcaaaactg acataaatct ccagagatgt
    20461 gttcaggagt tagaaagatt atttcttcta ttctagacaa atccccctct gattgacagc
    20521 atcactgacc aatcgcaaag attgctaagg ctgcttatgg cagggagata aggatggtcg
    20581 atacagatat ttggctgcgt ttaatgagca tcagcagctt gtacggcgat gatatggtcc
    20641 gtatagctca ctggctggca aaacagtcgc atattgatgc ggttgtattg cagcaaacag
    20701 ggcttacatt gcggcaggca caacgctttc tttcatttcc gcgaaagagt atcgaaagct
    20761 cactttgttg gttggagcaa cccaaccatc atttaatccc tgcggacagc gaattttatc
    20821 ctcctcaact tctggcgacg acagattacc ccggcgcact gtttgttgaa ggagaactgc
    20881 acgcgctgca ttcatttcag cttgccgtag tggggagtcg ggcgcattca tggtatggcg
    20941 agcgatgggg acgattattt tgcgaaactc tggccacgcg tggagtgaca attacgagtg
    21001 gactggcgcg tggaatcgat ggtgtggcgc ataaagcggc cttacaggta aatggcgtca
    21061 gcattgctgt attggggaac ggacttaata ccattcatcc gcgccgccat gcccgactgg
    21121 ctaccagttt gcttgaacat ggtggggcac ttgtctcgga atttcccctc gatgttccac
    21181 cccttgctta caatttccca cgaagaaatc gcattatcag tggtctaagt aaaggtgtac
    21241 tggtggtgga agcggctttg cgcagtggtt cgttggtgac agcacgttgt gcgcttgagc
    21301 aggggcgtga agtttttgcc ttgccaggac caatagggaa tccgggaagc gaagggcctc
    21361 actggttaat aaaacaaggt gcgattcttg tgacggaacc ggaagaaatt ctggaaaact
    21421 tgcaatttgg attgcactgg ttgccagacg cccctgaaaa ttcattttat tcaccagatc
    21481 agcaagacgt ggcattgcca tttcctgagc tcctggctaa cgtaggagat gaggtaacac
    21541 ctgttgacgt cgtcgctgaa cgtgccggcc aacctgtgcc agaggtagtt actcaactac
    21601 tcgaactgga gttagcagga tggatcgcag ctgtacccgg cggctatgtc cgattgagga
    21661 gggcatgcca tgttcgacgt actaatgtat ttgtttgaaa cctatattca cacagaagct
    21721 gagttgcgtg tggatcaaga caaacttgaa caggatctta ccgacgcagg atttgatcga
    21781 gaagatatct acaatgccct gctttggctg gaaaagcttg cggattatca ggaaggactg
    21841 acagaaccga tgcaactggc ctctgatcca ctctccatgc gtatttatac ccctgaggag
    21901 tgtgaaagac tggatgccag ctgtcgtggt tttctgcttt tccttgagca aattcaggtg
    21961 cttaaccttg aaatccgtga aatggtgata gagcgagtgc tcgcgctgga taccgcagag
    22021 ttcgacctgg aagacctgaa atgggtaatc ctgatggtgt tgttcaatat tccgggctgc
    22081 gaaaatgcgt accagcaaat ggaagaatta ctctttgaag tgaatgaagg tatgctgcat
    22141 taatcatttc tttaattcag cataagttgt tatggcgaaa tcagcactgt tcacggtgcg
    22201 taataatgag tcctgctcaa agtgcggggc tgaactggtt attcgatccg ggaaacacgg
    22261 tccgtttctt ggatgctcac agtatccggc gtgtgactac gtccgtcctc tgaaatcttc
    22321 agcggatgga catattgtca aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa
    22381 tctggtatta cgccagggac gctttggtat gtttattggt tgcagtaact accctgaatg
    22441 cgaacatacc gaacttatcg ataaaccgga cgaaacagca attacatgcc cccaatgtcg
    22501 gacgggccat ctggtccagc gccgctcccg ttatggcaaa acatttcact cctgtgatcg
    22561 ctacccggag tgtcaatttg ccattaactt caaacccata gctggagaat gccctgagtg
    22621 tcattatcca ctactcatcg aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag
    22681 taaacaatgt ggaaagccgg tttcggcgga ataataacgt gaataataac ctgcaaggag
    22741 acgctatcgc agctgcgata gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg
    22801 aagccgtttt cggtgttggg tgcgatcctg atagcgaaac agcagtgatg agactgttgg
    22861 agttaaaaca gcgtccggtt gataaggggc tgattttaat cgcggcaaat tacgagcagc
    22921 ttaaacccta tattgatgac accatgctga ctgatgcgca gcgtgaaacc attttttccc
    22981 gctggccagg tcctgtcacc tttgtctttc ccgcgcctgc gacaacaccg cgctggttga
    23041 cgggccgctt tgattcgctt gctgtacgag tcaccgacca tccgttggtg gttgctttgt
    23101 gccaggctta tggtaaaccg ctggtttcta ccagtgccaa cttgagtgga ttgccacctt
    23161 gtcgaacagt agacgaagtt cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg
    23221 aaacgggggg gcgtttaaat ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc
    23281 gacaggggta acataatgga aacctatgct gtttttggta atccgatagc ccacagcaaa
    23341 tcgccattca ttcatcagca atttgctcag caactgaata ttgaacatcc ctatgggcgc
    23401 gtgttggcac ccatcaatga tttcatcaac acactgaacg ctttctttag tgctggtggt
    23461 aaaggtgcga atgtgacggt accttttaaa gaagaggctt ttgccagagc ggatgagctt
    23521 actgaacggg cagcgttggc tggtgctgtt aataccctca agcggttaga agatggacgc
    23581 ttgcagggtg acaataccga tggtataggt ttgttaagcg atctggaacg tctgtctttt
    23641 atccgtccgg gtttacgtat tctgcttatc ggcgctggtg gagcatctcg cggcgtacta
    23701 ctaccactcc tttccctgaa ctgtgcggtg acaatcacta atcggacggt atcccgtgcg
    23761 gaagagttgg ctaaattgtt tgcgcacact ggcagtattc aggcgttggg tatggacgaa
    23821 ctggaaggtc atgagtttga tctcattatt aatgcaacat ccagtggcat cagtggtgat
    23881 attccggcga tcccgtcatc gctcattcat ctaggcattt attgctatga catgttctat
    23941 cagaaaggaa aaactccttt tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct
    24001 gatggtttag gaatgctggt ggcacaggcg gctcatgcct ttcttctttg gcacggtgtt
    24061 ctgcctgacg tagaaccagt tataaagcaa ttgcaggagg aattgtccgc gtgaatcagg
    24121 ccatccagtt tccggacagg gaagagtggg acgagaataa aaaatgtgta tgttttcccg
    24181 ctctcgtgaa tggtatgcaa ctgacatgcg cgatctctgg cgagagtctg gcgtatcgct
    24241 ttactggaga tacgccagaa cagtggttag cgagttttcg tcagcatcgc tgggacctgg
    24301 aagaagaagc ggaaaactta attcaggaac aaagtgaaga tgatcaaggc tgggtctggt
    24361 taccctgatc cagatattcg tccttccatt tcacgtaatt attcgcggaa tagcgtaacc
    24421 cagccttctc ttcatcactt aacgggcgga tctgtttgac ggggctaccg agatacagat
    24481 atccgctctc cagccgttta ttttgtggga ccagactacc cgcaccaatc atcacatcat
    24541 cttctactat tgcgccatca agtaaaattg agcccatccc aaccaaaact cgattgccaa
    24601 tggtgcagcc gtggagcatc accttgtgac caacagtgac atcttcgcca atggttaatg
    24661 ggttgccatc tgggttgtac gaggatttat gagtgacatg caacatactg ccatcctgga
    24721 tattggtgcg tgctccgatc tgtacataat gtacatctcc acgaatcaca acgagcggcc
    24781 agatccccac atcatcagcc agacgaacgt caccaatcac gacactgcta tcgtcgatca
    24841 ttacgcgctg accgatttgt ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac
    24901 ttacctcagc aataaatgat ttactaatga ctttggggac attattggcc ttgtgcaagt
    24961 cttttagtat gcaaaaaagc accgttttgt gtgcgattgc agcaaaaagg gtgaaaaaac
    25021 aacaaacaga aaaaaagatc aaaaaaatac ttgtgcaaaa tattgggatc cctataatgc
    25081 gcctccgttg agacgacaac gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa
    25141 aaatcctgaa attcagggtt gactctgaaa gaggaaagcg taatatacgc cacctcgcga
    25201 cagtgagctg aaggccgcgt cgcaac
//
LOCUS       DAJTMK010000044        24867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTMK010000044 DAJTMK010000000
VERSION     DAJTMK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24867
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.045"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..674)
                     /locus_tag="OO135_001549"
     CDS             complement(<1..674)
                     /locus_tag="OO135_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR5397451.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAVHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OO135_001550"
                     /pseudo
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="OO135_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OO135_001551"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="OO135_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5397452.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OO135_001552"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="OO135_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5397453.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OO135_001553"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="OO135_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5397454.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OO135_001554"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="OO135_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR5397455.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQDIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7757)
                     /gene="hyfF"
                     /locus_tag="OO135_001555"
     CDS             complement(6153..7757)
                     /gene="hyfF"
                     /locus_tag="OO135_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5397456.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVQMAAR
                     VLMAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHD
                     LPAQRSTWHDFLPSGTASVSEKHSER"
     gene            complement(7762..8412)
                     /gene="hyfE"
                     /locus_tag="OO135_001556"
     CDS             complement(7762..8412)
                     /gene="hyfE"
                     /locus_tag="OO135_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5397457.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8424..9531)
                     /locus_tag="OO135_001557"
                     /pseudo
     CDS             complement(8424..9531)
                     /locus_tag="OO135_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(9543..9653)
                     /locus_tag="OO135_001558"
     CDS             complement(9543..9653)
                     /locus_tag="OO135_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR5397458.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9670..10614)
                     /gene="hyfC"
                     /locus_tag="OO135_001559"
     CDS             complement(9670..10614)
                     /gene="hyfC"
                     /locus_tag="OO135_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5397459.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLTPLFTGIARQIRARMHSRRGP
                     GIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGG
                     DLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
                     STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEY
                     SGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVL
                     ASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10625..12642)
                     /gene="hyfB"
                     /locus_tag="OO135_001560"
                     /pseudo
     CDS             complement(10625..12642)
                     /gene="hyfB"
                     /locus_tag="OO135_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(12642..13259)
                     /gene="hyfA"
                     /locus_tag="OO135_001561"
     CDS             complement(12642..13259)
                     /gene="hyfA"
                     /locus_tag="OO135_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5397460.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQADGSLKDGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(13512..13982)
                     /gene="bcp"
                     /locus_tag="OO135_001562"
     CDS             complement(13512..13982)
                     /gene="bcp"
                     /locus_tag="OO135_001562"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5397461.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(13982..14554)
                     /locus_tag="OO135_001563"
     CDS             complement(13982..14554)
                     /locus_tag="OO135_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5397462.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14700..15578
                     /gene="dapA"
                     /locus_tag="OO135_001564"
     CDS             14700..15578
                     /gene="dapA"
                     /locus_tag="OO135_001564"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5397463.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15595..16629
                     /gene="bamC"
                     /locus_tag="OO135_001565"
     CDS             15595..16629
                     /gene="bamC"
                     /locus_tag="OO135_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5397464.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            16842..17555
                     /gene="purC"
                     /locus_tag="OO135_001566"
     CDS             16842..17555
                     /gene="purC"
                     /locus_tag="OO135_001566"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5397465.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     ELKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17723..18586
                     /locus_tag="OO135_001567"
     CDS             17723..18586
                     /locus_tag="OO135_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5397466.1"
                     /translation="MRWQGRRESDNVEDRRNSAGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            18601..20616
                     /gene="tmcA"
                     /locus_tag="OO135_001568"
     CDS             18601..20616
                     /gene="tmcA"
                     /locus_tag="OO135_001568"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5397467.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGCGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            20690..21388
                     /gene="ypfH"
                     /locus_tag="OO135_001569"
     CDS             20690..21388
                     /gene="ypfH"
                     /locus_tag="OO135_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5397468.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFHGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21498..21698)
                     /locus_tag="OO135_001570"
     CDS             complement(21498..21698)
                     /locus_tag="OO135_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5397469.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21726..22853)
                     /gene="dapE"
                     /locus_tag="OO135_001571"
     CDS             complement(21726..22853)
                     /gene="dapE"
                     /locus_tag="OO135_001571"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5397470.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(22857..23213)
                     /locus_tag="OO135_001572"
     CDS             complement(22857..23213)
                     /locus_tag="OO135_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5397471.1"
                     /translation="MVTLYGIKNCDTIKKARRCLEAYNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23594..23653
                     /gene="ypfM"
                     /locus_tag="OO135_001573"
     CDS             23594..23653
                     /gene="ypfM"
                     /locus_tag="OO135_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5397472.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23752..>24867)
                     /locus_tag="OO135_001574"
     CDS             complement(23752..>24867)
                     /locus_tag="OO135_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCR5397473.1"
                     /translation="PPAISGLGSSAGFDMELQDHAGAGHDALMAARNQLLALAAENPE
                     LTRVRHNGLDDSPQLQIDIDQRKAQALGVAIDDINDTLQTAWGSSYVNDFMDRGRVKK
                     VYVQAAAPYRMLPDDINLWYVRNKDGGMVPFSAFATSRWETGSPRLERYNGYSAVEIV
                     GEAAPGVSTGTAMDIMESLVKQLPNGFGLEWTAMSYQERLSGAQAPALYAISLLVVFL
                     CLAALYESWSVPFSVMLVVPLGVIGALLATWMRGLENDVYFQVGLLTVIGLSAKNAIL
                     IVEFANEMNQKGHDLFEATLHACRQRLRPILMTSLAFIFGVLPMATSTGAGSGGQHAV
                     GTGVMGGMISATILAIYFVPLFFVLVRRRFPLKPRPE"
BASE COUNT         6261 a         6581 c         6593 g         5432 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaag
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataacgaa
      121 cgcccgcaat aactcatcca cagcgccagg caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttaccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc acaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccgcaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccgatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttactgcg ggcaatttta
      601 tggacggcaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagatc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa cggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgtgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gcgcgattca tatggcgcgc cattttttgc gtgaaatgtt ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc aatgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgtcacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgttcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcgagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatgtg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggttt gtcgcgtcgc gtgctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacaccg cactgtcgct cttcgctaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgatg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtagtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttaccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctggca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgtt gttcttcatc attgagccat gattgcagaa ccggagtgat aatgcgtgcc
     2341 agccagttaa acagtgtgca atcctaggga gtaaaacggc tgccgtgggt acgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgataacatg cagttcaata agttgtaccg ggctgaacgg cggcggcatc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gtgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgtggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatctgcca gtagcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aggtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgacctgt gcaagccaat cttcgttttc cgtcagtggg cagcggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatggccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgt
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccacagc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca gattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcca
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaacaa ccttaatgcc aaaacgttct
     3721 gcgtcgaata cgggggtgat ggcagcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta ggctgagcga cggatatcct gtagtaaatg ccgcttcatc
     3841 ttttgcgttt gctcatccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgatcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccgcaacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcccca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg tgaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgtcctgag
     5041 tgcgttgttc catattcggc gtagcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccatcg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaaa ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgtagccg ttcgacttcc agcagaatcg
     5341 agcgaatggt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcggtccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccgacag ggatcacccg cgcatcgctg ttgccctcat
     5641 tgataaacgc atacgtttcg gaatctgtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ctgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgcagccatc
     6421 tgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtcctgc ggtaacggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgccagcgcc agcgccagcg ccagcgcacc gccgccaaac
     6601 agcacggcgg taaatggcat gattttgagc atcccacaga cgacgttgag atcgcgcgtg
     6661 ccgtacttga gcagtacatt gccggaaccg cagaacagca gcgtttttgc cagactgtgg
     6721 tttaaggtgt gcagcagcgc ggcaaaaatt cccagcggcc cgccaatgcc tagcgccacc
     6781 gcgaccagcc ccatgttctc cacgctggag tacgccagca gacgcttaat gtcccgctgt
     6841 accagaatga aaaatgccgc cacggcaacc gacaacatgc cgaagatgag caacaaccgg
     6901 ttggggaaat cgctgccgat ggcttggcaa ataatgatgt aatagcgaat cagcaccaac
     6961 agcgcgcagt tcagcaatac ggcggagagc agggcgctga ccggactcgg cgcttcactg
     7021 tgagcatccg gcagccaggc gtgcatggga aatagcccgg ttttggtacc aaagccaatt
     7081 agcacaaaca caaaggccaa cagcattaat gttgggtcaa gcaaggacga ttgcttaaga
     7141 acctcgctcc agaatatcgc catttctgcc tgcggcataa cgctggcggc gttggcgtat
     7201 accagcacgg taccgaacag accaaaagcg acaccaacag tacaaataat gatgtacttc
     7261 catgctgatt ccagcgatga acgctgaccg taaatgccta ccagaaacgc cgagcttaag
     7321 gtggtggctt cgatcgccgc ccacatcaca atcaggttat tgctggtaac aaccagcagc
     7381 atggtgaaca aaaacagatg gaagaaaccg tagtaatcgc acagtgtaac gggtgaaagc
     7441 tcgccgtgtg ccacttcatg acgcatgtag ccaatcgagt aaacaccggt gagaaagcca
     7501 atcacaccaa gaatggcgag gaacaaaccg cccagaccat caatatgaag ccacagtccc
     7561 gcagcgaata tttctcctgc atcagcggca gtttggacca cccagagtgc cagaatcagc
     7621 agcagtgtga tccctaagct atgtaataca gtcaccgtac aagtcgcaga gagcccccgt
     7681 ttccggcagg caaaacagag cagcgaaaaa agcagcggcg tgagcaggag taaagcgaac
     7741 atcacagaat aactcatctc attatccctt cagcgcggtc aagttgttca cgtccagcgt
     7801 gccgtgggta cgccatattt ttcttgccag taacaccatc acaatgacgg cgaagatggc
     7861 gtcggtagcg atacctattt ccaccagttc cggtgctcgc caggcaagaa gcgccagcac
     7921 cagatgggag ccgttttcca tcaggcagta accaaaaatt tgccgcagga tattgcgctg
     7981 gctgacaatg cacagcaggc caagcagaaa atgacctaac gctaccgcca gcgccggttt
     8041 cagcccggta gccatcggta gcttcacggg ctgaacgaca aatgcgcaaa gcaggacaat
     8101 taacgccgcg agcagtgcca tcattgccgg accgaataac gctttttccg ggatgttctg
     8161 gggaatattt cgtgcagcgt aagtcatgat taacggtacc agcagcactt tggtgataaa
     8221 ggcgctggcg gaccagatca gcagttgctc tgcggcgaac aggcacgaga gagtggcgaa
     8281 aatagacacc agcaccagcg actggcaggc gtaaaatccg catgacaggc gatagctttt
     8341 gacgctaatc acaaacagcg atgtcagcat catcagtccc gccagattat ttacgatcat
     8401 agaaccgatc attcatcatc tccttactgc aaccaggtcg cggcgattac gctggaaatc
     8461 agcgacatca caatcagtac aatcaacacc aggcgcattg accctggcag cggtgcggca
     8521 tcggcgacgg cctcgctcgg tttgccaggc acaacgcgac caaaccagcg aataaaccag
     8581 gcgaaactgg cgaccgattc aatcatcagc agaatcatgg cgggcagcag gatccagtac
     8641 tccactgaca acgcaaaacc ggcggcaaac agcgggaatt tactgaagaa gccgttgaac
     8701 ggcggcacac cggtaatcgc cagcgctgcc acgcagaaaa ccacgcctgg caacggcagg
     8761 gtgtgcaata cgccacgcag acgcggcaac aagcgcgtgc cgcagctgta actcagcgca
     8821 cctgctacaa ggaaaaacag gcttttagcg aacgcgtggt tgacgatgta ggcgatgctg
     8881 ccctccagtg ccagccgccc actttcacca tcgatgcggc gtggagatag gcgctgatcg
     8941 gtgttggcgc ttccattgcg tccggtagcc acgcttgcat cggtagctgg gccgatttcc
     9001 cccacgcggc aaacaggatg ccgccataaa ccagataacg tgcgtcgcca tgtaactcgc
     9061 tcatcgcgct aagcacaaac gttccggtct gcaaaaacag cgtggcggcg gcaagataca
     9121 accccaacga accgatatgg gtgataagca acgcttttag tgttgaacgc tgcgctttat
     9181 cgctctggta ataactgatc aacgcccagg agcagccgcc ctaatttcaa aaaacaacaa
     9241 ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa
     9301 aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata
     9361 acccgtggag tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt
     9421 actcacgcga tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa
     9481 cgtgacggca actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt
     9541 ggtcagtgcg gcgaacaaaa cccccattcg gcggcccgac gttgtggcga aaacgaaacg
     9601 accagtgcgc cgataaaagg cagcattaac gtcgtcagag caagattttc catattccgt
     9661 cagtgctcct tacagaccgg ttaaccagaa gacccatgca agcgcagcaa ggctgaagcc
     9721 aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt gccagcgtgt tttctgcgat
     9781 tgaggccagt acaaaaatca gcaaaacctt gagcagcgta acgacaagtg aagtcagcag
     9841 gcaggcgaga gaaagttctt gcgcgcgccc aaagggcaga aacagggcca caaacagtgc
     9901 tgccatcacg acctgtttca gccccagccc ccatttcgct agcgccagcc cggcaccgga
     9961 atattcggtc agcgggcctt cctgtaattc ctgttctgct tcagcaacat caaagggaat
    10021 ttttcccatc tcaatgaagc aggcaaaacc acaggccagt aacgccagta cggtggttag
    10081 cggcgagttc cagcccgttg ccagcgtatt gctgatcatc tcgatatgcg tggaacctgc
    10141 tatcagcgcc aataccagca gtgagagaat gagcatgggt tcgaccagaa tgccgagcgt
    10201 caactcgcga ctggcaccga ctcccgcaaa cgggcttccg gtatccagcc cggaaagagc
    10261 aaagaaaaaa cgaaacaggg caagaagata gataagggtg atcagatcgc cgccgcccgc
    10321 aaaaggggaa acggtaataa aaagtggtag ggccatcgcc agcaccagca tgctgctgat
    10381 taatacccac ggcatcaggc ggaacatcag acctgaagat ataggcgcaa cttcctggcg
    10441 tttaaacagt ttgtggatct cgcgataatc ctgccagatc cccggcccgc ggcgggagtg
    10501 catacgcgcg cgtatctgcc gggcaatacc cgtaaaaagc ggggttagca gcagaatcac
    10561 ggcctgtgct aacgcaaaaa tgaccagata tccgtcgcaa agagtttgtc tcatggtgat
    10621 ttccttagac ggcaatagcg attagcagca caaccagtgc ggcaactaga tacaggcaat
    10681 agagacgaaa gtcgccgctt tgcagatgct gtatttcttt ggccagccgt tgggtggcgc
    10741 tcacgatggg gcggatcacc cgctcatccc agaagggttc tgtgctctga gccctggcgg
    10801 tgacgtgcgc aagatcttta tttagcctca gcgtagggtc gagctgttgt cgtagacgaa
    10861 ataacgcaga gaagaccaca cgcagcggct gcatcacgcc attgcctgac ggagccatcg
    10921 cgttttccca gccgtagccg catgcccagg catctcctgt gcgacgaaac gctgcgcggc
    10981 gcgaacgaca ccacagccag taaaggccag gcagcaaagg catcgccagt agtaacagca
    11041 acagtaatga aggagtgacc cgtgtatgaa acgtgccggg tacaagtgct attccgctgg
    11101 cgacagtgac gggaggggta ttggtaaacg catgggcaat atgcattatc ttcggtgcca
    11161 gccaactggc actaagcgca attaatacgc agagtgccgc gagcagtagc atggcgacga
    11221 tcatcgtatt tggcacttcc tgtgcctctt cagcgtgtgt actgcgcggc gcaccacaga
    11281 aagtaatacc gtacattttg acgaagcaca ttactgccag cccaccagtg actgccagca
    11341 ttaccatagc aataggaccc gcaagttgta gcgctacggc ttccacacga cttagtgaga
    11401 acagcgactg ccaggtgtac cattcgctga taaaaccatt cagcggagga atggctgata
    11461 tcgcgaggca accaatcagg catgctgcgg ctgtccacgg catccgtttc gccagtgccc
    11521 ccattttttc catgtcgtgg gtatgcaaac gcgaaataat cgcgcccgcg ccgagaaata
    11581 gcagcccttt gaacagcgca tggttgagca gatgaaacag tgcgccgagc agtccaacca
    11641 cggtgagcag cgggtcgtgc agtgacagac cgtccatcgc cacaccgact gccagcaaaa
    11701 taatgccgac gttttcgacg gtactccagg ccagcagccg tttgatatcc tgttccgcca
    11761 gcgcatatag cacgcccagg agcgcggaga ttgcgccgat cgccatcacc agaatgcccc
    11821 accacagagg caaacccgtt tgcgccagca gatccatcgc tactttcagg atgccgaaaa
    11881 taccgatttt gaccattacg ccagacatca acgccgaagc gtgcgatggt gcggcagggt
    11941 gagcgcgcgg caaccagctg tgcaacggca tcatcccggc tttcgcgcca aaaccgaaaa
    12001 aggccagcag aaacaccgcc gacgccagcc ccggagaaag tgaaagcgtg cgaaactggc
    12061 aaaatcgagg ctgccgcttt cgcgccccat cagcaagaag gcgatcataa tcagcaccga
    12121 tccggcgtgg gcgataaaaa agtagagcat gccagcgttg attgacgttt tatcctgcct
    12181 ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga tgaaccaaaa
    12241 agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaagattca taaagaagcc
    12301 catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat agagcgaaca
    12361 aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat ctattcgcag
    12421 ggttaaatcg gcaaagtcaa aaggggtggc aaaacgggcg actaaagtta caccagcatg
    12481 ttattgcgta attccgctga tgatgccaat cacgccgccc accagcgatg tgatgccgga
    12541 aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac tggcaaacag
    12601 aaagaggatc agcgaccagg ttaataattg cagggcatcc attagtgttg ctcctgagtg
    12661 agggaaaggg gatcctcccc gccattggca aaccagcttg cggcaaggcg ctgtttttct
    12721 ttcatctgac gttgcaggct atcgtcggta atcagccgta acgcctgatt cgggcaagcg
    12781 cgaacgcagg ccggaccttc tggcaagaaa ttacacaggt cgcatttcac cgcgacggtc
    12841 tggacacccg gttcccagcg cagcaaagca tgttgggtcg gcgcgttttc ttcgccatct
    12901 ttgagtgagc cgtcagcctg aaaaacatat tgcgcagggg cattcaccgg acggcttcct
    12961 gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc aatacagagg
    13021 ctttcgttga gttggatcgc atcatccctc tgggagatgg cattgaccgg gcagacctgc
    13081 aggcaagggg cttcctcaca gtgatgacac acgacagggg cagtgattgt tgacgtcttc
    13141 gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc cgaacaggca
    13201 gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa gcggttcatg
    13261 tcggctcctt gaaaatgata tgaaacattg catttcatat tgcataaagg atgccagtct
    13321 gattaattta atatattcaa taaattatgt attaactggg ttctgaacgg tacaaaaatg
    13381 atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg gaaagcgaga
    13441 atgtgatctt gatcacgaga agtgaggtgt gctgaccgca agcgcagcca gcacggaatg
    13501 gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg gtgattgctg
    13561 gttttaaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag gaagctgatg
    13621 cgatgaatac catcgtaggt tttgcccatg aaggactttt caccccagac gccgaattgt
    13681 tcgcacacct ggtggtcctc atcagacaag agcgtaaagt taagcagctc tttttccgca
    13741 aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac gcccgctttt
    13801 ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc gggggtcatg
    13861 gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa attaacttgt
    13921 tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt cagtggattc
    13981 attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta atactgcctt
    14041 gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct gcgtccgcag
    14101 atgcggggct gtgggcggtg atctgaatat gcaactgcgc agcccgttca ttttcagcag
    14161 gttgcgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa agtgctgtga
    14221 agcgttcaat taaatgcggg gagtctgcaa catcgacctg aacccagaca gatgctggca
    14281 ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc agttcggcac
    14341 ctttcaacgg taacgttgat tcaatcagag taatggcatt ccatgaaccg gaaagcagca
    14401 tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata ttacagccgc
    14461 aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca cccaacgcag
    14521 tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta aggggtgagt
    14581 tgttcttaag gaaagcataa aaaaaacatg catacaacaa tcagaacggt tctgtctgct
    14641 tgcttttaat gccataccaa acgtaccatt gagacacttg tttgcacaga ggatggccca
    14701 tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt aatgtctgtc
    14761 gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg gcgatcgttt
    14821 ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct gatgtggtga
    14881 tgatgacgct ggagctggct gacgggcgca ttccggtgat tgccgggacc ggtgctaacg
    14941 ctactgcgga agccattagc ctgacgcagc gcttcaatga cagcggtatc gtcggctgcc
    15001 tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag catttcaaag
    15061 ccatcgctga acatactgac ctgccgcaaa ttctgtataa tgtgccgtcc cgtactggct
    15121 gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt atcggaatca
    15181 aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt tcagatgatt
    15241 ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaatta ggcggtcatg
    15301 gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg tgcaaactgg
    15361 cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg ccattacaca
    15421 acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag gaactgggtc
    15481 ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt ggtcgtgaga
    15541 cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga tttgatggct
    15601 tactctgttc aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttat tttattactc
    15661 gctgcctgta gttctgactc acgttataag cgtcaggtca gtggtgatga agcctacctg
    15721 gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc ggtgacctcc
    15781 ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc gctggacatt
    15841 cgtccaccag cccagccgct ggcactggtt tctggcgtgc gtacccagtt cacgggcgat
    15901 accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca ggtggttagc
    15961 gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca gacactgacc
    16021 atcgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg tcgttatcaa
    16081 atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgct gaacctggaa
    16141 caggcgggta aaccggttgc agacgcggct tccatgcagc gttacagcac ggagatgatg
    16201 aacgttattt ccgccggtct ggataaatct gcctctgacg ccgcgaacgc tgcgcaaaat
    16261 cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt accgatgctg
    16321 gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct ggaaaaagtg
    16381 ggcatgaaag tgaccgacag cacccgttcg cagggtaaca tggccgtaac ttataagccg
    16441 ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc atccggtgac
    16501 tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat cgatccgaaa
    16561 ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca ggctgcgttt
    16621 agcaagtaaa aatacagggc tggagtcatc cggccctttt ttctgatatg atacgcaaac
    16681 gtgtgcgtcg gcagaaaaat gcaattttag cggtaattcg cacgaaattt gtttgtcgga
    16741 cgtaggtcgg ataaggcgtt cttgccgcat ccgacaaaac atccggcaca ccagacaaca
    16801 aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag caagctgagt
    16861 tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg ttggtgctcg
    16921 aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt gatcgcaaag
    16981 gtatggtgaa caacaagttc aactacttca ttatgagcaa actggctgaa gcgggtatcc
    17041 cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag ctggatatgg
    17101 tgccggttga gtgtgtcgtg cgtaaccgtg ctgctggctc tctggtgaaa cgtcttggaa
    17161 tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa aacgatgcca
    17221 tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggtg agcaaagaga
    17281 acctggcgcg tatgaaagag ctgacctaca aagcgaacga cgagctgaaa aaactgttcg
    17341 atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac aaaggtgaag
    17401 tggtactggg cgatgagttc tccccggacg gcagccgcct gtgggacaaa gaaacactgg
    17461 agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgaa gcctatgaag
    17521 ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca tcttgccgtg
    17581 cagctggcac ggcaagacaa ccgctctcgg aatatatgca ttgaacgctc attcctccgc
    17641 catttcatcc cgctttcctg tggtaatctt cctctgaacc acctacgatc atttctataa
    17701 tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg acaatgttga
    17761 agacaggcgc aacagcgccg gtggtccgtc tatgggcggt cccggttttc gcctgccaag
    17821 cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact atggtgttga
    17881 tttaaccggg ttaatgactg gacagccggt ttcccaacaa caatcaacgc ggtccattag
    17941 ccctaatgaa gacgaagctg caaaattcac ctcggtgatt ctggcaacca cggaagacac
    18001 ctggggacaa cagttcgaga agatgggtaa gacctatcag caaccgaagc tggtcatgta
    18061 ccgtggaatg acgcgcaccg gctgcggggc gggccagtcc attatggggc cgttctattg
    18121 cccggcggat ggcacagttt atatcgatct ctccttctat gatgacatga aagacaaact
    18181 tggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg gtcatcatgt
    18241 gcagaaactg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg cgacgcaggc
    18301 ggaagtgaac cgcttatctg tgcgtatgga actccaggcc gactgttttg ccggtgtctg
    18361 ggggcataac atgcagcagc aaggcgttct ggaaaccggc gatctggaag aggcgctgaa
    18421 tgcggcgcag gccatcggcg atgaccgttt acaacagcaa agtcaggggc gagtagtacc
    18481 agacagtttc acacatggca cttctcagca acgctacagc tggtttaaac gtggtttcga
    18541 cagcggcgat ccggcacaat gcaatacttt tggtaaaagc atttaatttc cggggcaggg
    18601 atggctgaac tgactgcgct ttacacatta acagcgcaaa tgaaacgtga agggatccgc
    18661 cgcttgctgg tgttgagcgg ggaagagggt tggtgttttg atcatgcgct taagttgcgt
    18721 gatgccttac ttggcgactg gctgtggatt tcgccgcagc cagatgctga aaaccactgt
    18781 tctccctcgg cgctacaaac tttacttggg cgcgagttcc ggcatgcggt attcgacgcc
    18841 cgccacggct ttgatgccgc tgcctttgcg gcacttagcg gaacgttgaa agcgggaagc
    18901 tggctggttt tgttactccc tgtatgggaa gagtgggaaa accaacctga tgccgactcg
    18961 ctgcgctgga gtgattgccc tgaccctatt gcgacgccgc attttgtcca gcatctcaaa
    19021 cgcgtactta cggcggataa cgacgctatt ctctggcggc aaaaccagcc gttctcgttg
    19081 gcgcatttta ctccccgtac tgactggcac cccgcgaccg gcgcaccaca accagaacaa
    19141 cagcaactct tacagcagct actgaccatg ccgccgggcg tggcagcggt aacggctgcg
    19201 cgtgggtgcg gtaagtcggc gctggcaggg caactcattt ctcgtattgc gggcagtgcg
    19261 attgtcaccg cgcccgcaaa agcggcaacg gatgtactgg cacaatttgc gggcgagaag
    19321 tttcgcttta ttgcgccgga tgccttgtta gccagcgatg agcaagccga ctggctggtg
    19381 gtcgatgaag ccgcagccat acctgcgccg ttgttgcatc aactggtatc gcgttttcct
    19441 cgaacgttgt taaccactac ggtgcagggc tacgaaggca ccggacgtgg ttttttgctg
    19501 aaattttgcg ctcgctttcc gcatttacac cgttttgaac tgcaacagcc gatccgctgg
    19561 gcacagggat gcccgctgga aaaaatggtt agtgaggcac tggtttttga cgatgaaaac
    19621 ttcacccata caccacaagg caatatcgtc atttccgcat ttgaacaaac gttatggcga
    19681 agcgagccag aaacgccgtt aaaggtttat cagttattgt ctggtgcgca ctaccggact
    19741 tcgccgctgg atttacgccg gatgatggat gcactagggc aatatttttt acaggcggct
    19801 ggcgaaaacg agattgccgg agcgctgtgg ctggtggatg aggggggatt atctcaacaa
    19861 ctcagtcagg cggtatgggc aggttttcgt cgcccgcggg gtaatctggt ggcccagtcg
    19921 ctggcggcgc acggtagcaa tccactggca gcgacattgc gtggacggcg ggtcagccgg
    19981 atagcagttc atccggctcg tcagcgcgaa ggcgttgggc aacagctcat tgccagcgct
    20041 ttgcaatata cgcaagacct cgactatctt tcggtgagtt ttggttacac cggggagtta
    20101 tggcgtttct ggcaacgctg cggttttgtg ctggtacgaa tgggtaatca tcgtgaagcc
    20161 agcagtggtt gctatacggc gatggcgctg ttaccgatga gtgatgcggg taaacagctg
    20221 gctgaacgtg agcattaccg tttacgtcgc gatgcgcaag ctctcgcgca gtggaatggc
    20281 gaaacacttc ctgttgatcc actaaacgat atcgtgcttt ctgacgacga ctggcttgaa
    20341 ctggccggtt ttgcttttgc tcatcgtccg ctattaacgt cgttaggttg cttattgcgt
    20401 ctgctacaaa ccagtgaact ggcattaccg gcgctgcgtg ggcgtttaca gaaaaatgcc
    20461 agtgatgcgc agttatgtac cacacttaaa cttgcaggcc gcaagatgtt actggtccgt
    20521 cagcgggaag aggccgcgca tgcgctgttc gcacttaatg atgttcgcac tgagcgtctg
    20581 cgcgatcgca taacgcaatg gcaatttttt cactgactcc ttcaattttc tcccatggtc
    20641 attgtgagcc agcggcgtaa aattaatgac atcagttaag gagttcgcca tgaaacatga
    20701 ccattttgtt gttcaaagcc cggataaacc tgtgcaacag ttgttactgc ttttccacgg
    20761 tgtcggggat aactcggtgg caatggggga aataggcagt tggtttgcac cgctgtttcc
    20821 tgatgcgctg gtggtgagtg tcggtggcgc ggagccgagc ggtaatccgg cggggcgtca
    20881 gtggttttcg gtgcagggta ttacggaaga taatcgccag gcgcgtgtgg atgccatcat
    20941 gccgacgttt attgaaacgg tacgctactg gcagaaacag agtggagtag gggctaacgc
    21001 cacggcgtta atcggtttct ctcaaggcgc gattatggcg ctggagagta ttaaagccga
    21061 accgggcctt gcttcacgcg tcattgcttt taacggacgc tatgcgagcc tgccggaaac
    21121 cgcgtctaca gcaacgacaa ttcacctgat tcatggcggt gaagatccgg ttatcgatct
    21181 ggcgcatgcg gttgcggcgc aggaagcgtt aatcagtgcc ggtggtgacg tgacgctgga
    21241 tatcgtggag gatttaggcc atgcgatcga caatcgcagt atgcaatttg ccctggatca
    21301 cttgcgttat accattccga aacattattt cgacgaagcg ctaagtggcg gtaagcctgg
    21361 cgatgatgat gtgattgaaa tgatgtaaaa gacagattac ctggcctgat aagacgcgtg
    21421 agcgtcgcat caggcatcgt tcaccattgc cggatgcggc gttaacgcct tatccggcct
    21481 acggttgagc actcagctta cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg
    21541 tcccacttat cgttgaaatc acgatgcggg ggaagctccg gcttgttggc gagaaatttc
    21601 ttgtggtcga cgcgcttgag atctttaatc acgttcagca ggacgcctac caaaaagaca
    21661 atcaccagaa tccaccaata tttagccagc cagtccatgc ttatttcctc ttgcagaacc
    21721 actcatcagg cgacgagctg ttccatgata cgttgataca tacgggcaag tagctgcagg
    21781 tcggcagcgt tcacacattc attaatttta tgaatagtgg cattgaccgg cccgagttcc
    21841 accacctgcg cccccatgcg agcaataaag cgcccgtcgg acgttccgcc tgtggtcagt
    21901 agctgcggtt taatttcatt atagtgctca accgcgttaa cgaccgcatc caccagttta
    21961 ccgcgcgcgg tcaaaaatgg ctgcccggaa agccaccaat ccaccgtata gcgcagttga
    22021 tgtttttcaa gcagggcaag cacctgcgct ttgatcatct catcagtcag ttcggtgctg
    22081 aagcggaagt taaactgcac aaacagttca cccggaataa cattgttact gcccgttccc
    22141 gcctgaatat tagcaatctg catactggtc gccgggaaaa attcattgcc ctgatcccac
    22201 tctatagcca ctaattcatt aaggaaaggt gctgcgcgat gtactggatt gtcagccaga
    22261 tgcgggtagg ccacatgccc ctgaacgcca tgaatggtaa ggttgcaggt taacgatcca
    22321 cgacgaccat ttttcaccac atcaccaacc acttcgatac tcgacggttc gccgaccagg
    22381 cagtaatcga gacgctcatt acgtgccatc aacgcttcga cgacttttac cgtaccgttg
    22441 tgggcactgg cttcttcatc agaggtgatc agaaatgcca gtcgccccgt atggttggga
    22501 tgttgtgcga caaagcgttc tgccgctacc accatcgccg ccagcgagcc tttcatatct
    22561 gccgcaccgc gcccgaataa catgccatca cgaatggtgg gttcaaacgg cggattgatc
    22621 caacgatcgg cgtcgccagg cggcaccacg tcggtatgcc cggcaaaggc taacgtttca
    22681 ccttgcccac gccatgccca aaaattctgc gtatcggcaa agtccatgcg ttcaacggta
    22741 aaaccgatcg cctgcaaacg ttcaatcaac aaagcctggc atcctgcgtc atcaggactc
    22801 agggaagggc ggcgaataag ctgttgtgtc agctcaataa ccgggcacga catagactac
    22861 acctcatgga aaaattgctg ataactggaa tcactgaaac ccagcagcat aggcttacca
    22921 ggcgcgcaga gcaatggacg tttgataatt gaaggcattt cagtcattaa tgccgccgca
    22981 gaggccgcat cggtgatttt attgcgggtg gtttcgtcca gtttacgcca ggttgtacca
    23041 cgggtgttca gtaacgcttc ccagcctaat tcgttgataa aaccgttcaa taattcgctg
    23101 tccagcccat cgacgcggta atcatgaaaa cgatagtcga tgttataggc ttccagacaa
    23161 cggcgagcct ttttaatggt gtcacaattt ttgatgccgt aaagtgtaac cataatgaat
    23221 cctttttcga tctatgagaa taaatactca tttaagggtg aaaacatttt gcctgcaaat
    23281 gattatcagg aaatataaca ttatcgaagt attcaaatta acaactatta aataatatta
    23341 aacattttta acgtaaacat taagaatatt catcttgtta tctgtctgta ttttaaccag
    23401 aattaaatca gtattagatg ccgtatgaat atctcgaaat gttgctgtgt atcacataca
    23461 aattatcacc gcaggaccat agtatttaca taaggtttgt cccccggagg tagtcatcgc
    23521 agcaatcgag taaattagct tcaccacggt atattgtttc cgtagaatac ccataattat
    23581 aagagaggtt gttatgattg aacgtgaact ggggaactgg aaagacttta tcgaagttat
    23641 gcttcgtaag taattcctga gacaaagtac aaacagcaag aacccgctga gcaggttctt
    23701 aatcggaaag ttgaaaaagg cgacacatta gcatgtcgcc ttttttattg cttattccgg
    23761 gcgcggcttc agcgggaagc ggcggcgcac cagtacaaag aacagcggca cgaagtaaat
    23821 agccagaata gtggccgaaa tcatcccgcc cattacgcca gtacccaccg catgctgacc
    23881 accggaaccg gcacccgtgc tggttgccat tggcaatacg ccgaagataa atgccagcga
    23941 ggtcatcaga atcgggcgta aacgctgacg gcaggcgtgg agcgtcgctt caaacaggtc
    24001 gtggcctttt tggttcatct cgttagcaaa ctcgacgatc aggatggcgt ttttcgccga
    24061 taaaccaatg accgttaaca ggcccacctg gaagtaaacg tcgttttcca gcccgcgcat
    24121 ccaggttgcc agcagcgcgc cgataacccc cagcggcacg accagcatta ccgagaacgg
    24181 caccgaccag ctttcataca acgccgccag acacaggaat accaccagca aggaaatggc
    24241 gtacagcgcc ggagcctgcg cgccggaaag ccgctcctga tacgacatcg ccgtccactc
    24301 cagaccaaag ccgtttggca actgcttcac taacgattcc attatatcca tcgccgtacc
    24361 ggtactgacc cccggtgcgg cttccccaac aatctcaacc gcagaataac cgttatagcg
    24421 ttccagacgc ggcgagccag tctcccagcg tgaggtcgcg aaagcagaga agggcaccat
    24481 gccgccatct ttatttcgga cataccagag attaatgtca tctggcagca tgcgatacgg
    24541 cgcagctgcc tgcacataga ctttcttcac acgaccgcga tccataaagt cattcacata
    24601 gctcgaaccc caggcggttt gcagtgtgtc gttaatatcg tcgatagcaa cgcccagcgc
    24661 ctgagcttta cgctggtcga tatcaatctg caactgcgga ctgtcatcga ggccgttatg
    24721 gcgcacacgg gttagctccg ggttttccgc cgccagcgcc agcaactggt tacgtgctgc
    24781 catcagcgca tcgtgacctg ctccagcgtg gtcctgcaac tccatatcaa aacccgcaga
    24841 actaccaaga ccgctaattg ctggcgg
//
LOCUS       DAJTMK010000045        24655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTMK010000045 DAJTMK010000000
VERSION     DAJTMK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24655
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.046"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..808
                     /locus_tag="OO135_001575"
     CDS             <1..808
                     /locus_tag="OO135_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR5397474.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFVPETKGKTLEELEALREPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO135_001576"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO135_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5397475.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(1376..1519)
                     /locus_tag="OO135_001577"
     CDS             complement(1376..1519)
                     /locus_tag="OO135_001577"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397476.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            1520..1798
                     /locus_tag="OO135_001578"
     CDS             1520..1798
                     /locus_tag="OO135_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397477.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSFRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="OO135_001579"
     CDS             complement(1845..3941)
                     /locus_tag="OO135_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5397478.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLDQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="OO135_001580"
     CDS             complement(3941..4678)
                     /locus_tag="OO135_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5397479.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(4675..5313)
                     /locus_tag="OO135_001581"
     CDS             complement(4675..5313)
                     /locus_tag="OO135_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5397480.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="OO135_001582"
     CDS             complement(5427..5669)
                     /locus_tag="OO135_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397481.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO135_001583"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO135_001583"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5397482.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPVEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="OO135_001584"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="OO135_001584"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5397483.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="OO135_001585"
     CDS             9906..10178
                     /locus_tag="OO135_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5397484.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO135_001586"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO135_001586"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5397485.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12478
                     /locus_tag="OO135_001587"
     CDS             11531..12478
                     /locus_tag="OO135_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5397486.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            12548..13354
                     /locus_tag="OO135_001588"
     CDS             12548..13354
                     /locus_tag="OO135_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5397487.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWSTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVTDFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            13364..13771
                     /locus_tag="OO135_001589"
     CDS             13364..13771
                     /locus_tag="OO135_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5397488.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLGKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13771..14265
                     /locus_tag="OO135_001590"
     CDS             13771..14265
                     /locus_tag="OO135_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5397489.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYQNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14331..15128
                     /locus_tag="OO135_001591"
     CDS             14331..15128
                     /locus_tag="OO135_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5397490.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15139..15960
                     /locus_tag="OO135_001592"
                     /pseudo
     CDS             15139..15960
                     /locus_tag="OO135_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            16015..17244
                     /gene="sorE"
                     /locus_tag="OO135_001593"
     CDS             16015..17244
                     /gene="sorE"
                     /locus_tag="OO135_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5397491.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHSSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17627..18316
                     /gene="pepE"
                     /locus_tag="OO135_001594"
     CDS             17627..18316
                     /gene="pepE"
                     /locus_tag="OO135_001594"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5397492.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18407..20038)
                     /locus_tag="OO135_001595"
     CDS             complement(18407..20038)
                     /locus_tag="OO135_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5397493.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20258..23408)
                     /locus_tag="OO135_001596"
                     /pseudo
     CDS             complement(20258..23408)
                     /locus_tag="OO135_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            23608..24432
                     /gene="iclR"
                     /locus_tag="OO135_001597"
     CDS             23608..24432
                     /gene="iclR"
                     /locus_tag="OO135_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5397494.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5827 a         6483 c         6427 g         5918 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgt
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctccgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcagccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgtt
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccggca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgatccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaagccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagctgttt gccagccgcg gttggcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac cagggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aagtcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa atttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttcttta tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcgacaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tctgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ttgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag gtcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gtagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtac tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcgtatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt
    11821 acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg
    11881 gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt
    11941 aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa
    12001 cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc
    12061 ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac
    12121 catctctgcc tactgggata atctggatgt cgccctggtg ggaattggct caccggccat
    12181 tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc
    12241 ccgccaggtt gctggcgata tttgctcgcg cttttttgat attcacggcg caatggttga
    12301 aacgaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata
    12361 ttccattggc attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg
    12421 aaaatatatt aattgtctgg taacgaatag cagcacagct gaactattac tgaaataaaa
    12481 atcttcccga tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga
    12541 gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc
    12601 atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat
    12661 ggtagatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggtc
    12721 gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt
    12781 tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga
    12841 tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa
    12901 tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca
    12961 acatgatggc gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca
    13021 aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga
    13081 attgggtaaa cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg
    13141 actgcgcacg ccggaatatg aagaagcgct ggcgtggacg cgcaatatca ccgtcgagca
    13201 gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga
    13261 agtcactgat tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac
    13321 cactaacatt gcgggcggca aaacgcgcgg ctaaggaggc tttatggtca atgccatttt
    13381 ttgcgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga
    13441 cgccagggtc gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa
    13501 gctgggaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca
    13561 gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt
    13621 aatcagcggc ctttctctgc cactggcgct cgaactggtt gataaccagg acagcatgaa
    13681 tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa
    13741 acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac
    13801 cgcctgatcc acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt
    13861 attatctgca atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct
    13921 gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcaa
    13981 aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta
    14041 gcgatggttc gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg
    14101 cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt
    14161 catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca
    14221 atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg
    14281 tacatatgct ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa
    14341 gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc
    14401 tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg
    14461 gtgatttaaa aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga
    14521 tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc
    14581 tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg
    14641 cggcaggcca ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg
    14701 cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt
    14761 ttggcgtgca ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg
    14821 ccgatatggt gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa
    14881 ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga
    14941 aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc
    15001 tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac
    15061 agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg
    15121 acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg
    15181 tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt
    15241 ttttgctacg acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt
    15301 gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc
    15361 atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt
    15421 accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg
    15481 gtctggggaa cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc
    15541 aacattctcg gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag
    15601 tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg
    15661 aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg
    15721 ctggtcacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc
    15781 gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg
    15841 ctggcgctcg gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta
    15901 tggctgattt tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc
    15961 agattcttcg ccccggcacg actgccgggg ccatcgctca acatgaggtg gtttatgaaa
    16021 acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct
    16081 gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc
    16141 tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac
    16201 cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag
    16261 cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc
    16321 ccggactgcc ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt
    16381 cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa
    16441 ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt
    16501 caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc
    16561 ggcggcaccg gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac
    16621 ccgtcgcagc tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac
    16681 tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat
    16741 acgctaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat
    16801 gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc
    16861 gccggaccgc aggataaaca ttccagcgcg ccaattaatt tctacgatgt gcattatgca
    16921 tttacccact acgtgggcac gtcaggtggc aataccgacg acatgcgcgc ggcggtcaaa
    16981 ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat
    17041 gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat
    17101 accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg
    17161 attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat
    17221 gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg
    17281 ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag
    17341 aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
    17401 tttatgccgg atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata
    17461 ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
    17521 agtctcagct cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
    17581 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
    17641 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
    17701 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
    17761 ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
    17821 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt
    17881 cggcggcggg aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc
    17941 aattactgac gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct
    18001 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
    18061 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
    18121 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
    18181 gacgattatt ggtctaccgg aaggtaactg gatcacagtg agtaaaggtc acgctacgct
    18241 gggtggcccg aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc
    18301 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
    18361 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
    18421 gtccttcatc ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt
    18481 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
    18541 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
    18601 agcgatgctt actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
    18661 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
    18721 catcaagcga aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc
    18781 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc
    18841 ggcgcgactc ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg
    18901 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
    18961 caccgtgcat cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca
    19021 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca
    19081 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
    19141 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
    19201 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
    19261 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
    19321 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
    19381 aaccgaggtt agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg
    19441 cggtcagagt cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
    19501 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
    19561 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
    19621 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
    19681 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
    19741 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
    19801 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
    19861 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
    19921 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
    19981 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
    20041 gttcaggttc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
    20101 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
    20161 ccagcctgat tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
    20221 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    20281 tcggtgccag ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt
    20341 caacctgatc gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat
    20401 accaacccga aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg
    20461 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    20521 ccgggtagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    20581 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tgaagatact
    20641 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    20701 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    20761 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    20821 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat
    20881 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    20941 ggcccaccac gccacgcgga ttcagcgttt tctcggcgct taatttatcc agcatgtcgt
    21001 tggcgtcttt aaacagccgc tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg
    21061 gatacttccc ggccagcgac caggtcataa agaacggtgt ccagtcgatg taattacgca
    21121 gagtttcgat gctggcttca acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt
    21181 aagcctgcca gtcaaaagcg aagtcgttat cgcgcgccgc ttccagcgtg accggtggtg
    21241 tgcgcggttt cttgcgcccg tgctgaatac gtacggtttc gtactccttg cgggtacgag
    21301 cgacaaaatc atcgcgctga gtatcggaaa gcagcgccgc caccacacca acggtgcgcg
    21361 aggcattctg cacatacacc gtcgggccgc tgtagttctg ctcaattttc accgccgtgt
    21421 gcgcttttga ggtggtcgcg ccaccgatca gtaacggaat ggtgaagccc tgacgctcca
    21481 tctctttcgc cacgttaacc atctcgtcca gcgacggcgt gataagcccc gaaaggccaa
    21541 tcaaatcagc attcacttct ttagcagtac ggagaatttt ttccgcaggc accatcacgc
    21601 cgagatcgac aatttcgtag ttgttacatt gcagcaccac gccaacgata tttttaccga
    21661 tatcgtggac gtcgcccttc acggtggcga tcaccatctt gccgttggtt ttgccctgct
    21721 ctttgctggc ttcaataaac ggttcgagat acgccaccgc ctgtttcatg acgcgcgccg
    21781 ataaagtcct gcgcgtagtt gttgtgctct tcaataccgg tggcgaccgc gaagatgttc
    21841 gggtcgaaga tgatatcttc tggcgggaag ccaacctctt cggtgaggat tttgtacgcc
    21901 cgacggcaaa tctcgatttt ccgtgcgcga gtatcggcct gtccctgttc gtcaaaggcc
    21961 atcaccacca ctgccgcacc gtagcgacgc aacaatttcg cgtgatggat aaaggcgtcg
    22021 acgccctctt tcatcgagat tgagttaaca atgcctttgc cctgaataca cttcagccct
    22081 ttttcgataa cgtcccattt tgaggagtcg atcataatcg gcacgcgagc gatatccggt
    22141 tcaccggcaa tcagattgag aaaacgcacc atcgccgctt cggcatcgag catcccttca
    22201 tccatgttga tatcgataat ctgcgcgccg ttttccacct gctgacgcgc cacatcctgc
    22261 gcctcgctgt atttctcttc tttgatcagg cgcttgaact tagcagagcc ggtgacgttg
    22321 gtgcgttcac ccacgttcac aaacaggcta tcgtcgccaa tgttcagcgg ctccaggcca
    22381 gacaaacggc aggctacggg aatttccggc agtttgcgcg gcgctaatcc ttctactgca
    22441 cgactcatcg ctgcaatatg ttgcggcgtg gtcccgcagc aaccaccgac gatattgaga
    22501 aagcccgctt gcgcccattc acgtatctgt tttgccatcg tgtcggcgtc gagatcgtat
    22561 tcaccaaagg cgttaggcag cccggcgttc gggtgggcgg tgacgtagca ttccgcaatc
    22621 cgtgatagtt cctgcacgta ctggcgcagt tcatcgggcc ccagcgcaca gttcaggcca
    22681 aaggttagcg cttcggcgtg gcgcaatgag ttgtaaaatg cttcggtggt ctgcccggag
    22741 agcgtgcgcc cggaggcgtc ggtgatggtg ccggagatca taatcggcag ctcaacgccc
    22801 agcgcttcaa actccgtttt caccgcaaaa accgccgctt tggcgttaag ggtgtcgaaa
    22861 acggtttcaa tcaggatcag atccgcacca ccttccacca gcgctttggt ggattcgcga
    22921 taggccgcca ccagctggtc aaaagtgatg ttgcggaatg ccggatcgtt gacgtccgga
    22981 gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg caacgtagcg cggtttctcc
    23041 ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca gtttcgccgc cgcaaagttg
    23101 atttccgccg acagggattc catctggtaa tccgccatcg caatggtcgt ggagttgaag
    23161 gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg cgttgtggat agcggcgatc
    23221 acttccggtt tgctgagcac cagcaggtcg ttgttgcctt tgagatcgca tggccagtcg
    23281 gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat aactctggat catggtgccc
    23341 ataccgccgt ccagcaccag aatacgttca tttaactgcg cacgcagttg ttccactttg
    23401 ctgctcacac ttgctcccga cacgctcaac ccagatttaa taaaaattca acaaaccata
    23461 ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca acatgagatt tgttcaacat
    23521 taactcatcg gatcagttca gcaactattg cattagctaa caataaaaat gaaaatgatt
    23581 cccacgatac agaaaaagga gactgtcatg gtcgcaccca ttcccgcgaa acgcggcaga
    23641 aaacccgccg ttgccaccgc accagcgact ggacaggttc agtctttaac gcgtggcctg
    23701 aaattactgg agtggattgc cgaatccaat ggcagtgtgg cactcacgga actggcgcaa
    23761 caagccgggt tacccaattc cacgacccac cgcctgctaa ccacaatgca acagcagggt
    23821 ttcgtgcgtc aggttggcga actgggacac tgggcaatcg gcgcacatgc ctttatggtc
    23881 ggcagcagct ttctccagag tcgtaatttg ttagcaattg ttcaccctat cctgcgcaat
    23941 ctaatggaag agtctggcga aacggtcaat atggcggtgc ttgatcaaag cgatcacgaa
    24001 gcgattatta tcgaccaggt acagtgtacg catctgatgc gaatgtccgc gcctatcggc
    24061 ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct ttttagccca actgagcgaa
    24121 gaacaggtga cgaagctgct gcaccgcaaa gggttacatg cctataccca cgcaacgctg
    24181 gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc gcaaacgggg ttattcattt
    24241 gacgatgagg aacatgcgct ggggctacgt tgccttgcag cgtgtatttt cgatgagcac
    24301 cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt cacgtattac cgatgaccgc
    24361 gtgaccgagt ttggcgcaat ggtgattaaa gcggcgaagg aagtgacgct ggcgtacggt
    24421 ggaatgcgct gactttttct ggcgggcaga ggcaatattc tgcccatcat acctgagtgg
    24481 caatagaata agggtgtctg ttaatcgtat tgacgccaaa ataacttaat gttatacact
    24541 tcacacactt ttattaactc ggcattattt ttaaacatca aaccacttta attattaaca
    24601 aaatcgcaag atctcattcc gccgaaactt ttacggtttc attatgagat tgagc
//
LOCUS       DAJTMK010000046        24472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTMK010000046 DAJTMK010000000
VERSION     DAJTMK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24472
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.047"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(119..850)
                     /gene="rlmB"
                     /locus_tag="OO135_001598"
     CDS             complement(119..850)
                     /gene="rlmB"
                     /locus_tag="OO135_001598"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5397495.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1031..3472)
                     /gene="rnr"
                     /locus_tag="OO135_001599"
     CDS             complement(1031..3472)
                     /gene="rnr"
                     /locus_tag="OO135_001599"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5397496.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3511..3936)
                     /gene="nsrR"
                     /locus_tag="OO135_001600"
     CDS             complement(3511..3936)
                     /gene="nsrR"
                     /locus_tag="OO135_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5397497.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVSCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4141..5439)
                     /gene="purA"
                     /locus_tag="OO135_001601"
     CDS             complement(4141..5439)
                     /gene="purA"
                     /locus_tag="OO135_001601"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5397498.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5543..5740)
                     /locus_tag="OO135_001602"
     CDS             complement(5543..5740)
                     /locus_tag="OO135_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5397499.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5822..6826)
                     /gene="hflC"
                     /locus_tag="OO135_001603"
     CDS             complement(5822..6826)
                     /gene="hflC"
                     /locus_tag="OO135_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5397500.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6829..8088)
                     /gene="hflK"
                     /locus_tag="OO135_001604"
     CDS             complement(6829..8088)
                     /gene="hflK"
                     /locus_tag="OO135_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5397501.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8174..9454)
                     /gene="hflX"
                     /locus_tag="OO135_001605"
     CDS             complement(8174..9454)
                     /gene="hflX"
                     /locus_tag="OO135_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5397502.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9531..9839)
                     /gene="hfq"
                     /locus_tag="OO135_001606"
     CDS             complement(9531..9839)
                     /gene="hfq"
                     /locus_tag="OO135_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5397503.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9925..10875)
                     /gene="miaA"
                     /locus_tag="OO135_001607"
     CDS             complement(9925..10875)
                     /gene="miaA"
                     /locus_tag="OO135_001607"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5397504.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10868..12715)
                     /gene="mutL"
                     /locus_tag="OO135_001608"
     CDS             complement(10868..12715)
                     /gene="mutL"
                     /locus_tag="OO135_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5397505.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTSALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12725..14062)
                     /gene="amiB"
                     /locus_tag="OO135_001609"
     CDS             complement(12725..14062)
                     /gene="amiB"
                     /locus_tag="OO135_001609"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5397506.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14081..14542)
                     /gene="tsaE"
                     /locus_tag="OO135_001610"
     CDS             complement(14081..14542)
                     /gene="tsaE"
                     /locus_tag="OO135_001610"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5397507.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14514..16061)
                     /gene="nnr"
                     /locus_tag="OO135_001611"
     CDS             complement(14514..16061)
                     /gene="nnr"
                     /locus_tag="OO135_001611"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5397508.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDVLLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16060..17199
                     /gene="queG"
                     /locus_tag="OO135_001612"
     CDS             16060..17199
                     /gene="queG"
                     /locus_tag="OO135_001612"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5397509.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGVIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17182..17235)
                     /locus_tag="OO135_001613"
     CDS             complement(17182..17235)
                     /locus_tag="OO135_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397510.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17464..17539)
                     /locus_tag="OO135_001614"
     tRNA            complement(17464..17539)
                     /locus_tag="OO135_001614"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17504..17506),aa:Gly,seq:gcc)
     gene            complement(17576..17651)
                     /locus_tag="OO135_001615"
     tRNA            complement(17576..17651)
                     /locus_tag="OO135_001615"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17616..17618),aa:Gly,seq:gcc)
     gene            complement(17862..18407)
                     /gene="orn"
                     /locus_tag="OO135_001616"
     CDS             complement(17862..18407)
                     /gene="orn"
                     /locus_tag="OO135_001616"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5397511.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18502..19554
                     /gene="rsgA"
                     /locus_tag="OO135_001617"
     CDS             18502..19554
                     /gene="rsgA"
                     /locus_tag="OO135_001617"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5397512.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19651..20619
                     /gene="psd"
                     /locus_tag="OO135_001618"
     CDS             19651..20619
                     /gene="psd"
                     /locus_tag="OO135_001618"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5397513.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREIIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20641..23964
                     /gene="mscM"
                     /locus_tag="OO135_001619"
     CDS             20641..23964
                     /gene="mscM"
                     /locus_tag="OO135_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5397514.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24115..24429)
                     /locus_tag="OO135_001620"
     CDS             complement(24115..24429)
                     /locus_tag="OO135_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5397515.1"
                     /translation="MESLPTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVV
                     VVLVVTFANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELA
                     LD"
BASE COUNT         5619 a         6859 c         6230 g         5764 t
ORIGIN      
        1 ccatatagat ttagtttaaa tattaataaa atgaatattt gcagtacgta attatcttac
       61 cagctataga caaaaaaaaa ccatccaaat ctggatggct tttcataatt cagaggaact
      121 agctgcgctg acgcaccgct tcaaataagc aaattccggt tgcaaccgaa acgttcagag
      181 aagaaacact tcctgccatt gggatgctga tcaactcatc gcaatgttca cgggtcaggc
      241 gacgcatacc ttcaccttcc gcgcccatca ccagcgccag gcgtccggtc attttgctct
      301 gatagagtgt atgatccgcc tcacctgccg taccgacgat ccagatattc tcttcctgca
      361 acatacgcat ggtacgcacc aggttagtca cccgaatcag cggaacgctt tctgccgcgc
      421 cacaggccac ttttttcgcc gtggcgttga gctgggcgga gcgatctttc ggcacaatca
      481 ccgcatgaac accagcggca tctgcgctac gcaggcacgc gccgaggtta tgcggatcgg
      541 ttacaccgtc gaggatcagc aggaacggct gatcgagcga agcgatcaga tccggcaaat
      601 cattttcctg gtactgacgt cctggcttca cgcgggcgat aatgccctga tgcacggcac
      661 cgtcgctttt ctcgtcgaga tattggcggt ttgccagctg gataaccacg ccctgggctt
      721 cgagggcgtg aatcagcggt aacagacgtt tatcttcacg gccttttaaa ataaagactt
      781 cctgaaaacg ttcaggggcg cgctccagca gggcctgcac tgcgtggatg ccgtaaatca
      841 tttcgctcat taatgtactc gttgtttacg ttttgcctga tgcactacgt ttatccggcc
      901 tacatgatct ctgcaatata ttgaatttgc gtgattatgt aggccggata aggcgttcac
      961 gccgcatccg gcatgaacaa agcgcatttt gtcagcaatc taaccctctt ctgtttgaag
     1021 agggtaatga tcactctgcc acttttttct tcgccgcacg cttcgctttg gtcgctgcag
     1081 ctattttctg cgttttcgcc gatggctttt tcgcttttct cgcgtctttc ttcgctgctt
     1141 tcggcttcgt ttttttctca ccgcggaagg cgctgtctgg ctcaaagttt acctttttac
     1201 cgaccagacg acgcttgccg ccttttttac ctgcatcgcc ttttttcgct ttctcgcgcg
     1261 ccgttttacc gacgttgcgc ggtgcgcgtt cgctggagat caggctaaag tcgattttgc
     1321 gctcgtccat attaaccgct tcgacgcgaa cttccacgcg atcgcccaga cgataagtct
     1381 ggccgctgga ttcacccatc agccgttgcc ctacctggtc aaagcgatag tagtcattgt
     1441 ccagcgaaga gacatggacc agaccatcaa tgaataagtc gtccagacgg acaaagaatc
     1501 caaagccagt gacgctggaa attacgcctt taaagacgtt acctacctgg tcgagcatga
     1561 agtcacactt cagccagtca gccacatcgc gcgttgcttc gtcggcacga cgttccgcca
     1621 tcgaacagtg ctgacccagt tgcagcattt cttccatcga ataatggtag ccgccggttt
     1681 cagtggtgtt gccctgatgc ccctgctctt tcgccagcag atatttaatg gcgcggtgca
     1741 gcgtcaggtc tggataacga cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca
     1801 ggccaaagtg accacggttt tctggatcgt aaatcgcctg tttcatcgag cgcagcagca
     1861 tggtttgcag catttctgca tcaggacgat cggcaaccga ctccagcagc tccgcgtagt
     1921 cacgcggttc cggcttgtta ccgcccggca gttccagccc cagctccgcc agtactgaac
     1981 ggaaagaggt aatcgcttcg gtgctcggct tgtcgtgaat acggaacaat gctggttctt
     2041 tggctttctc aacgaaacgc gccgccgaga tattcgccag aatcatgcac tcttcaatta
     2101 acttgtgtgc gtcgttacgc tgagtctgtt cgatacgctc aatacggcat tcagcgttga
     2161 aaataaactt cgcttcttcg ctctcaaatg agatcccgcc acgttcttca cgggctttat
     2221 ccagcacttt atagaggtta tgcaactctt cgagatgctt aaccagcgga gcgtactgct
     2281 cgcgcagatc ctgatcgccc tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt
     2341 gagagctcat caccgcttcg tagaatttgt agcccgtcag gcggcctttc gacgaaaccg
     2401 tcatctcgca caccatacac aggcggtcta cctgcggatt gagcgagcac agaccgttag
     2461 agagcacttc cggcagcatc gggataacct gcgaagggaa gtacaccgac gtaccacgat
     2521 tacgcgcttc tctatccagc ggcgttggcg gacgcacata gtagctgacg tcggcaatcg
     2581 cgacccataa acgccagccg ccgccgcgtt ttttctcgca gtaaaccgca tcgtcaaagt
     2641 cacgggcgtc ttcgccatca atggtgacca gcggtaaatc acgcaaatcg acacggcccg
     2701 cttttgcttc ttccggcact tcttctttca gcccggcaac ctgttgctca acagcctgcg
     2761 gccagatgta cggaatttca tgggtacgca aagcgatatc aaccgccatg ccggtgccca
     2821 tattgtcgcc cagcacttcg acgattttac ccaccgcttt ggtacggcga gtcgggcgtt
     2881 gggtcagttc aacaaccacc acaaagccca tccgcgcgcc catgatctga tcgggcggga
     2941 ttaagatatc gaagctcaga cggctatcgt caggaaccac aaagccgacg cccgcttcgg
     3001 taaagtagcg accaacaatc tggctggttt ttggcaccag tacgcggaca atacgcgctt
     3061 cacgacgacc tttacggtca gcacccagcg gctgagccag cacctgatcg ccatgaatgc
     3121 aggttttcat ctgctcgctg gagagataca aatcatcttt acgcccttca acccgcagaa
     3181 agccgtagcc atcacggtgg ccaataacgg tacctttcac caggtcgagg cgttccggca
     3241 gcgcatagca ctgacgacga gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc
     3301 ggcgacgcag gccttcaagc tgctcttcgc cttcaatgtg cagttctacc gccagctcat
     3361 cacggctggc cggtttttca cgtttggtta aatgttcgag gataaattcc cgactaggga
     3421 tgggattcgc gtatttttca gcttcgcgtt cctggaaagg atcttgtgac atctcggttc
     3481 ctccgttgtc atctctgatg aagattttcg tcactccacc agcaataatt tataaagcgg
     3541 ttgattctct tcaaccaaat cggcaagcgt gtagttatcc agttccgtaa gaaaactttg
     3601 cacggcctta gaaagcgcct gtttcaaccg acaggcaggt gtaatgtggc aaaactcact
     3661 gctgcaactc accagcgata agggctccaa ctcgcgcacc acatcaccaa tacgtatcgc
     3721 actcgccggt ttacccaggc gaatgccgcc attttttcca cgaacagcag tcacgtagcc
     3781 ggcacgacta agttgattga ttattttgac catatgatta cgggagacgc cgtagacgtc
     3841 agtcacttca gaaatactgg tcatccgccc ttctggcaat gacgccatgt agatcagcgc
     3901 acgtaatccg taatcagtga aactcgttaa ctgcacatca acctcaaaag ggaaatcggg
     3961 aaaaaataca tttatattga tgataaacca gccacaagct gtgtcgctaa tttatttcag
     4021 ttcggggaag gaaaaaagcg aggatttaac actgtgtcgg atagcgtaat ggcttaaccg
     4081 acacagcaat gtatcacact gttagcccgg caggcaaaat atctgccagg cgtaccagaa
     4141 ttacgcgtcg aacgggtcgc gcagaatcat agtttcagta cgatccggac cggtagagat
     4201 gatatcgatc ggcacaccgg tcagctcttc aatacgcttg atgtagttca gcgccgcctg
     4261 cggcagcccg ctacgatctt tcacgccgaa agtggattca gaccagcccg gcatggtttc
     4321 gtaaatcggc tctacacctt tccagtcgtc agctgccagc ggagtggtag tcacttcgcg
     4381 accatccggc atacggtaag ccacgcagag tttaacctct ttcaggccat ccagaacgtc
     4441 cagtttagtc aggcagaagc cagacaggga gttcagctgt accgcacgac gaacggcaac
     4501 ggtgtccagc cagccggtac gacgacgacg acccgtcgtt gcgccgaatt cgttaccctg
     4561 cttgcagagg aactcgccag tttcatcaaa cagttcggtc gggaaaggac ctgcacccac
     4621 acgagtggag taagctttga ggatacccag aacgtaatca acataacgcg ggcccaggcc
     4681 ggaaccggtc gccacgccac cagcagtggt gttggaagaa gttacgtacg gataagtacc
     4741 gtggtcgata tccagcagcg taccctgcgc accttcaaac atgacgaaat cgccacgctg
     4801 acgcgcctgg tcgagcaggt cagaaacgtc aaccaccata gaagtcagga tgtcggcaac
     4861 agccatcgta tcatccagaa ctttctggta atcaaccgct tcagctttgt agtagttaac
     4921 caactggaag ttgtgatatt ccatcacttc tttcagtttt tcagcgaagg tttctttgtc
     4981 gaaaaggtcg ccaacacgca gaccgcgacg tgccacttta tcttcataag caggcccgat
     5041 accacgaccg gtggtgccga tcgctttcgc gccacgcgct ttctcacgcg cgttatccag
     5101 cgcaacgtga taatcaagga tcagcggaca tgcttcagac agcagcagac gctcacgaac
     5161 ggggatgcca cggtcttcca gttctttcat ctctttcatc agcgcggccg gagacagcac
     5221 aacaccgtta ccgatgatgc tggttacatt ctcgcggaga atacctgatg gaataagatg
     5281 gagaacggtt ttttcaccgt tgattacgag agtatggcct gcgttgtgac cgccctggta
     5341 gcgtacaaca tatttagccc gttcagtcag aagatcgacg atcttacctt taccttcgtc
     5401 accccattgg gtgcccagta cgacgacgtt gttacccatt tttcaaaatc accgtttgct
     5461 taaaaatgga ttctaccatc gctttttcag agttacagca ctttttgcac tcaaaaatga
     5521 cctcgatcag tctgtttttt gttcagccaa tcgttttcct caacatgtag tagaccacaa
     5581 cgcccgcaac cacaagtcca ccgccaaaac gacgtaaaat attatcgggc aaattggtca
     5641 tcgcagagat catcttcttc catgccttcg ggtaaagcat cggccctaaa ccttccagta
     5701 ccaaaaccag ggcaagcgcc agccagattg tcgaattcat tcattatcct tatagaaaaa
     5761 gaaaaccacc gacatccctg aggatgcggt ggctttattg acctgtaccg cagtcgttat
     5821 attaacgcgt tgcggaagtc ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca
     5881 tgaccatcac gtcctgattg ccagagaagc tgttctcata agcacgcagg ctacggatga
     5941 atgcgtagaa gtccggatct ttactgaatg catcagcaaa cagtttggct gcttcggcat
     6001 cgccttcacc acgcatgatg cggccctgac gctcagcttc tgccagcgtt ctggtcactt
     6061 catagtcggc agtcgcgcgc agtttttccg cttcttcctg accttgtgaa cggtgacgac
     6121 gcgctaccgc ttcacgctcg gcgcgcatac ggttgtagat cgcttcagac acttcggtcg
     6181 gcaggttgat ctgcttgata cgcacatcga caacttcaat acccagcgcc gccatactgt
     6241 tcgggttgat gaccggaact ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg
     6301 caatggcgtt atctgctgcc ggggtagtaa cttcatcttc tgtacccgca gaaccggagt
     6361 tcagcgcgtc acgtacttcg agggtcagac gaccacggga atcagtgacg atatctttca
     6421 cgtccaggcg accaatttca gaacgcagac ggtcagagaa cttacgtttc aacagcactt
     6481 ccgcttgcga aatgtcgcca ccacccgttg ccaggtagta acggctgaaa tcgctgatgc
     6541 gccatttgat gtaagagtcg acgatcaggt ctttcttctc tttggtcaca aagcggtcgg
     6601 cctggttgtc catggtctga atacgtgcgt cgagcatttt caccgtttca atgaacggta
     6661 tcttgaaatg cagacccggc tcataaacca gaggtttgtt gtcatcgtca cgcagtacct
     6721 taccaaaacg cagcgtaata ccgcgctcac cttctttgac gacaaagaca gacatgtaaa
     6781 gcactaccag cacgatgatg ataatcgcga taactgactt acgcatcgtt attccccctg
     6841 acgctggtag tcgttacgct gcgcgttggc gcggcgttgg tccataatat cgccctgact
     6901 ggtggacgac gtgttgcttg ctccactggt tgtggaggaa gaggctggcg gcaaacgcag
     6961 cagattgctg gcaccgttat cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg
     7021 gtctaacggc agaaccatca ggttgccacc tttatcgtta accagcactt tgcgggtgtt
     7081 acccaacact ttttccatcg tctcgatata cagacgctcg cgagtaattt ccggcgcggc
     7141 tttatattcc ggcagaagtt tagcaaagcg cgccacttca ccctgagctt ccaggatggt
     7201 ctgggccttg tacgcacgcg cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg
     7261 ctgaacttcg ttggtatacg cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc
     7321 ggcaatcgca tcgtcaaacg ccgcttttac ttcttccggc ggacgagcag cctggaagtt
     7381 gacgtccagc agagtgatgc ccatgtcata cggacgaatc gtctcttcca gttcgcgctg
     7441 ggtatcgcta cgaatcacgg tacgaccttc cgtcagaatg cggtccatgg tgtatttacc
     7501 gataaccccg cgcagggcgc tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac
     7561 gctatacaga tatttttccg gattggtaac gcggtactgc acgttcatct caacgcgcac
     7621 tacgttctcg tccgacgtca gcatcacacc ggaagcggcc agttcacgca cggcttccac
     7681 gtttaccggt ttgacttcgt cgataaacgt cggtttccag ttcagacccg gctcaaccag
     7741 atggctgaat ttaccaaagc gtgttaccac gccgcgttcg gcttctttaa tggtatagaa
     7801 accactggcc gcccagataa tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc
     7861 aagctgcggg cgcgggcctt gcgatgaact gccaccgcca gatccggtgc ctttaccgcc
     7921 gcccagacca ccgagctttt tgctcagttt gcggaagata tcatctaaat caggtggccc
     7981 ttgatcgcga ccgcctttgt ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc
     8041 ccacgggtcg cggtcttgtc cgttattacc gggctgattc cacgccatgt ttgtgctcca
     8101 tatttgttat gcggtgatcc ctgttgtctt tcgacgccag aggatatgac tccacgcttc
     8161 agacgctacg ccgttagatc aggtaatcga tcaacgccgg ttcttgttta cagaggcgac
     8221 gccagtcaac gatcggcata cgaacttgca gacttacgct gccgtcctcc tccatccact
     8281 ctttttctat tgcctgaagc tgataaaaac gacttctcag acgcccttcc tgcggtggca
     8341 gacgcaatgt atgctgcgcc acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct
     8401 gtggtatccc cgctccggtc tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt
     8461 cccgatcaat acgcggttcg aaatcttcca gcatatcgat cttgttcatc accagcaggg
     8521 ttgggatctc gtgagcgtcg atctcctcaa gaaccgtatt caccgcttcg atgttttctt
     8581 gtacacgcac atccgccgca tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt
     8641 gtaacgtggc tttaaatgcc gccaccagat cgtgcggcag atggcgaata aaccctacgg
     8701 tatctgcaag tacggtttca ccgacatccg cgacgtcaat acgccgcaac gtcgggtcga
     8761 gggtggcaaa caactggtct gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg
     8821 tagatttacc ggcgttggta tatcccacca gcgaaacagt aggaacgtcg gctttgatac
     8881 gcgattgccg cccctgctca cgctgctttt caactctttc caggcgcgac tgtatctgca
     8941 cgatgcgatt acgcaacaaa cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca
     9001 aacctatccc gcctttctgt ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca
     9061 gatggcgcag ctgcgccagc tcaacctgca acttaccctc atgggtacgc gcacgttggg
     9121 cgaaaatatc taaaataagg ccggtgcggt cgatgacacg acactcgcac aaacgctcca
     9181 ggttacgctc ttgcgccggg ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg
     9241 ctttgacagc ttccgcaatt tcaactgctt taccttcacc tacaaaatac tttgggtgcg
     9301 gcgctttacg gctaccggta atcacctgca atgcttcgac accggcggaa gaaaccagag
     9361 attcaaactc ctggaggtct tccatatctt tgtctttcgt aaaatagatg tgtaccagta
     9421 ccgcctgctc accagcatca taacggtcaa acaagcgtat aaccctctaa atagatcagc
     9481 ggggaacgca ggatcgctgg ctccccgtgt aaaaaaaaca gcccgaaacc ttattcggtt
     9541 tcttcgctgt cctgttgcgc ggaagtattc tgcgcgctgc taccatgatg gtagttactg
     9601 ctggtaccgc caccggcgtt gttactgtga tgagaaaccg gacgagacgg gacaacagta
     9661 gaaatcgcgt gcttgtaaac catctggctg accgtgtttt tcaacaggat cacgaactga
     9721 tcaaaagact cgatttgccc ttgcagctta ataccattca ccaaataaat agaaactgga
     9781 acacgttccc gacgcagtgc gttcaggaac ggatcttgta aagattgccc cttagccatt
     9841 ctctcttttc cttatatgct tatttgtact ttgaaccttt cgattctgaa aaaattgcgc
     9901 acgatacgtc tcaattgtac acattcagcc tgcgatagca ccaacaacct gtaatacttc
     9961 gtcacgcgcc tgttctggtt tttcactgtc aagccagtga accccttccc aaccacgcag
    10021 ccaggttatc tgccgcttcg ccaactgtct cgtggcgcaa acacctcgat aaaccatttc
    10081 atcgtatgag atttcgcctt caaggtaaga ccacatctgg cgataaccca cgcaacgaat
    10141 ggaaggcaag tccgtatgca aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa
    10201 acctgaagcc aacatctgat gaaaacgctg ctcaatgcgt tgatggagca gttcacggct
    10261 cgccggggcg atggcgaact gatgcacctg atacggtaga gcgtctcctg acgtttgcgt
    10321 cagttccgtt aaagttttac ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg
    10381 tggatcattt ggatgaatcc ttgctgccgc aaccggatct acctcctgaa gttgacgatg
    10441 caatgactcc caaccttgct ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc
    10501 tgccgacggt agcggcgaca acccttccag caatgccttg aaatacaaca tcgtaccgcc
    10561 cactaacagt gggatccgcc ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg
    10621 gcgaaaatca gcagccgagt aagcctgcga cggatcgcga atatccagca atcggtgcgg
    10681 cgcggcgagt aactcttcag cgttcggctt cgccgtcccg atatccatcc ctttgtaaat
    10741 aagggcagaa tcaacgctta tcaactctac tggtaaaatt ttacgcagct caatggctaa
    10801 cgccgtttta ccggaggccg tcggccccat caaaaaaatc gccttaggca ggctcgcctt
    10861 actgatatca ctcatctttc agggctttta tcgccggatg taaatcaaca gattgtaaca
    10921 gaccacccgg cggcgttttc acaagttgcg gacataaccg ttccacgtcc gccagcaggg
    10981 ttatggcctg tgccattgac cactgcgcat gttcgctcat cagatttcgt gcaatccact
    11041 gcgcaatatt gccaggttcg aatacggact gcttcgccag gtagcctatc agttcaggaa
    11101 tcaagatttg taaattttgt tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct
    11161 gtgcatctga ctggaaatca atacccaatt ccgccagggc agactgcgct ttttctaatg
    11221 ccgatttttc ttcgccagaa acttttagcc gcaacggaat aagcagcggc tgagcgcaaa
    11281 ctggcgcttc acccggcgtc agttgtacct gacgcagcca acgttctgcc actggcaagg
    11341 ctaaaagtga aatgttgccg tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca
    11401 gtacccgacc aaaactctga ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt
    11461 ttaatttttg catcggcgcg ggcgtttgca aaagctggcg atacacttca ccttgctgtt
    11521 tctggtagcc tggctgcgca ttcggccaag gggcagccgg acgactgcct gatgctggcg
    11581 caggagtgta gcgcggagct accggctcac gagctgccgg ttctgcaaag tgattgcgcc
    11641 ccgccgccac gcggttttcc ggaatggaac gcggtgcagg ttggggttca tcgtccagcg
    11701 gtagcggcgt ttccagttgc tgttgtagca cgctcagcac gccctgatag ataaaatcat
    11761 gcaccagacg cgactgatgg aaacgcactt cgtgtttggc ggggtgcacg ttgacgtcca
    11821 cctgatgcgg atcgatctcc agatacagca caaatgccgg ttgctgatcg gcccccagtt
    11881 tgtcttcgca ggcctggcgg atcgcgtgat tgatcaggcg atcgcgcatc atgcgaccgt
    11941 tcacgtagca atactgaatt tctgccagtg cggacgtggt gtgatttgga tcggccaccc
    12001 agccgcgtag cgtgagatcg ccgtgttgcc attcaatcgc cagcgcttgt tcaagaaaag
    12061 cggtgccgca aatcgcgcct aagcgccgtt ctttttgccc gccttccggc actgcgcggt
    12121 actgacgcac aattttaccg ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg
    12181 caatgcggcg gatgatctca tcaatgtggt taaattcggt tttctcggtg cgcaggaatt
    12241 tgcgccgcgc cggggtgttg tagaacagat ccagcacctc cagcgtcgtc cccacaggat
    12301 gcgccgccgg ttttaccgtc acgttcatat cgcgcccttc ggcataggcc tgccaggctt
    12361 cctgctgttc tgcggtgcgt gaagtgagcg tcaggcggga aaccgaactg atactcgcca
    12421 gcgcctcacc gcgaaagccc aggctgataa tggcttcgag atcgtccaga gaggcgattt
    12481 tactggtggc atgacgagcc agcgccagcg ccagctcatc ttttttgata ccgcagccgt
    12541 tatcacgaat gcggataagt ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg
    12601 cacctgcatc gaggctgttt tccaccagtt ctttgactac cgacgcaggt cgctcgacca
    12661 cctcacctgc ggcaatctgg ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc
    12721 gtccttagtt tggcagcgtg cgatctggcg tcgtcaccgt actggccgtt tgtgccgttg
    12781 caccctgcgg cgcagattgc atcggatgcg caaggaaata attgcgcagg cctttgtaaa
    12841 tggcttctgc cagctgttgt tggtaatcgt cgctcgccag caaacgttct tcgctgttgt
    12901 tgctgataaa accggtttcg accagtactg acgggatatc cggcgaacgc agaacgccga
    12961 gactcgcgtg ttctggtcgg cgtttatgta tttcgccaat gcgttgcaac tgactgatca
    13021 tactggtcgc tacatcatac cctacccgct gggaatgacc gaactgtaaa tccagcaccg
    13081 cctggcttaa atagggatca gactgactgt tcgccagcac atcgcccgcc ccacccagca
    13141 gctccgaatg tttctcgtgc tgctccagcc agctggccat ttcgctgttg gcgcgacggt
    13201 tagagagcac ccatacggaa gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa
    13261 tcgacacgag gaaattagcg ttttgcttac gtgccacatc gctgcgcccc atcaccgaaa
    13321 taaagtaatc cccgtcacgg gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag
    13381 tacgcaattt acgcgcgatg gcgatggtga catttttctc ccgcgtacca ccggggccga
    13441 tagcgccagg gtcctgaccg ccgtgtccgg catcaatagc gatgataatt ttatcgccag
    13501 tgttagccgt cgcgcgcgct gccggacgcg ttaccgtatt actgctgata acacccgtag
    13561 tgcggttact ttccgtttta aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa
    13621 ccgcaggcgt ttcaacgcgt ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat
    13681 cggcgttaat cgtaaagacg acagtgtaat tgctgccatt ctgccgcttc accgcttcgg
    13741 ttttaccatt ttcggtaaga tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc
    13801 cagagcgaat cgccttcacc agattattgc cgctgaacaa caacggcagt ccctgaatca
    13861 cgcccgtttg tttgatatcg agcgccacgg tgcgtttgct ttgatggcta aacgcataat
    13921 caggatcgcc aataaaactc aacgttatcc gcgcctgttg attaccgtta gaaacctgaa
    13981 tatcagagag cgtcgcggca cccaccggcg tgcacagcag cagcagcgtc gctaccaacc
    14041 aatttctgat gcgatacatc atcccgccac ctttcaaagg ttaaccggct aaacgcgcca
    14101 gcaacaattc acccgcagag gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat
    14161 aatcaatgtg tatttcgaca tccgggtcag gaagaacacc tgtaccttgt tgtggccact
    14221 ccaccaggca gatggcatcg ttggcaaaat aatcgcggat ccccataaac tccagctcct
    14281 cgggatcggc aaggcggtac aaatcaaagt gataaaccat taaattgtcg agcgtatagg
    14341 gttcgaccag cgtataagtg gggcttttga cattaccctg atgacccaga gcttgtaaaa
    14401 agccccggct aaaggtggtc ttacctgcgc ctaaatcgcc atacagatag attacggttg
    14461 cgccatcgca ggctttcgct acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga
    14521 gcggaattac tcgattcatc atggttttta tcagtcactt ccgggttaac aatacgctgt
    14581 agcgtggaaa agagatcggt tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg
    14641 tcagctgccg caccgtgcgc gacacagccc gcacaggcgg catcatacgg cgacagtttt
    14701 tgcccaagca atgcgccaat aataccagag agcacatcgc ccatgccgcc gctcgccatg
    14761 cctgcatttc cggcatcaat aatgcctaaa gcgtcaggat gggcggcgac cacggttccg
    14821 gcacctttca gcaccgctac gccgccataa cgttgtacca gacgtttggc gcaatgtaag
    14881 cggtcacttt caatttcagc gacggaacag cctaacaacc gtgcggcctc gccaggatgc
    14941 ggcgtgatca cgcgattgtg acgcttatcg ggattgattg ccagcaggtt caatgcatcg
    15001 gcatcccaca acatcggttt gcgaaaattc tcaacttttt gcagtgcttt tttcccccac
    15061 tcttgctggc ccagaccggg accaatcacc accacatcgg cccattccag gctttcggta
    15121 agagagtcca tcgtcagttc atgcaccatc aattccggtc gtgcagtcag cagcggcgca
    15181 atgttttcac tgcgggtcag tactcggact aaaccagcac cagcacgcag cgccgcttcc
    15241 cccgtcatac gaatagcccc cgccgtgccg tgatcgccac cgataattac cagccgcccg
    15301 tgatcgcctt tatgcgaagt cgggcgacgc ggttttagcc agtgagaaag ttgttctgct
    15361 gaaaaccgct gaattttcgt ctcctgacct gccagccaac tatccagccc cagtgagtca
    15421 aaatgcagtt gtccggtaac atcccgcgct tttccagtga gcaagcctgg tttcagcgca
    15481 ataaaagtga tggtgtgatc ggcgttgatc accgcgcctg gcgtagcgcc agtttcagcc
    15541 agcaggccgg aagggatatc aaccgccaca atcggcgcag gatgggaatt agcgtggtcg
    15601 attaactggc taatggattc gcggggcgct tgccgcaaac cggtaccgag cagcacatca
    15661 acaatcagat ctaccgattc gggccagaca atattcgaag catggatctc gccacccgcg
    15721 tttaaccatg cttcgcgtgc cagcgcggcc tcttccggca acggtttgtc gctctcctgg
    15781 gcaaacaacg tgacctcaat gccgaccgct ttggccagtc gcgcgaccac gtagccatcg
    15841 ccgccgttat taccatgacc gcacagcacc agccagtggc gggcgtcagg atacgccgaa
    15901 cgacacacct ggaatgcggc ctcgccagcg cgaagcatca gctcatagag tgtgagcccc
    15961 agcacatctg ccgcctcgcg ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt
    16021 ggtatactta cggggttttt cttcattgta tggtccgtca tgtcagagcc cctcgatctc
    16081 aatcagttag cgcaaaaaat taaacagtgg gggctggaac tgggctttca gcaggtaggt
    16141 attaccgata ccgatctcag cgagtccgag cccaaactgc aagcatggct ggacaaacaa
    16201 taccacggcg aaatggactg gatggcacgt cacggtatgc tgcgcgctcg ccctcatgag
    16261 ttattgcccg gtacgctgcg cgtgatcagc gtgcggatga attaccttcc tgctaacgcc
    16321 gcatttgcca gcacgctgaa aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc
    16381 cgtgactatc acaaacttct gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa
    16441 cattgtgttt cgctgaattt tagaccgttt gtcgattctg cgcctattct cgagcgcccg
    16501 ttagctgaaa aagctgggct cggctggaca ggtaagcact cacttatcct caatcgcgag
    16561 gccggttcgt tcttcttttt aggagaattg ctggtcgata ttccgctgcc cgtggatcaa
    16621 ccagtcgagg aaggatgcgg taaatgcgtg gcctgtatga cgatttgccc gaccggtgcc
    16681 atcgtcgagc catataccgt cgatgctcgc cgctgtatct cttatctcac catcgaactt
    16741 gaaggggtga tcccggaaga gttgcgaccg ttaatgggaa accgtattta cggttgcgat
    16801 gactgccagc ttatctgccc gtggaatcgc tattcacaac tcaccacaga agaggatttc
    16861 agcccgcgta agccgctaca cgcaccggaa ctcattgagt tattcgcctg gagcgaagag
    16921 aagtttttaa aagtcacgga aggatcggcg attcgtcgta ttggtcacct gcgttggctg
    16981 cgtaatatcg ccgtagcatt aggcaatgcc ccttgggatg aaacgatttt gacagcgctg
    17041 gaaagtcgta aaggtgagca cccacttctc gatgagcaca tagcgtgggc gattgcgcag
    17101 caaatagaga gacgaaatgc gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg
    17161 gttcgggtga ttgaaaaagg gttaccgcgt gacgcctgat tcatccacag cttgtgaata
    17221 aaaataaaaa cgcattgcaa ttcaagacga ataaaatcgt caagtgatcg actcgacaaa
    17281 acggaatctt aatttttaac ttatctatca ataggttaaa aaaatactat tcaccgtgcg
    17341 aagtttcttt gaaattaaga ttacagcctg aggctgtgga taactctgtt tacaagagtt
    17401 tgtcagagac aacgaaaacc agcaataacg catcgctgtg gatattttat tgagagaaga
    17461 atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
    17521 ccaactgagc tattcccgct tgggtggtct gtgccttaca gcacttttca aattttggag
    17581 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
    17641 gctattcccg cgtactactt aattttgctt tcgtaatttt cgcatttctg cgtcgttacg
    17701 ggaggcgcat tatacgagaa atctttccct ctgcaacccc ccaaaaaagt tttttttgcg
    17761 tttttatctc aagtgctgaa attatcagca agacgttcaa tttagcgtca aaatcacgcc
    17821 gccgcaagtg gattccggcg cttacgtgac caggaaaaat tttacagctt gataaaatgc
    17881 tcgcggtagt aagccagctc cgccaccgat tcacggatat catccatcgc ctgatgcgtc
    17941 ccctgcttgg taaaaccatc cagaatttcc ggcttccagc ggcgcgccag ctctttcagg
    18001 gtgctgacat cgagataacg gtagtggaag taggcttcca gctccggcat gtatttaaac
    18061 aggaaacgac ggtcctgtcc gatgctgtta ccgcaaatcg gcgattttcc cgcaggcacc
    18121 cactgtttta agaattcgag cgttgccagt tcagcttccc gatcgcccat cgtgctcgct
    18181 ttcacgcgct ctaccagccc gctggcggta tgggtgcgca cgttccagtc atccatcagc
    18241 gccagctgtt catcggactg atgtactgca atggtcggcc cttctgccag aatattcagg
    18301 ttggcatcgg tcaccagcgt ggcaatctca ataatgcgat cgcgctcggg atccagacca
    18361 gtcatctcaa gatcgatcca aatcaggttg ttttcattgg cactcatgct attttccacc
    18421 cattcacagt gactatattg atccacaatt gcgtgtatca tagatgtttt gcccatcagg
    18481 ggcgaccagg agtctgtacg attgagtaaa aataaactct ccaaaggcca gcagcgccgc
    18541 gtgaacgcca atcaccagcg tcgtcttaaa acgtctaagg agaagcccga ctacgacgac
    18601 aatctgtttg gcgagcctga tgaaggtatc gtcatcagcc gctttggtat gcacgctgat
    18661 gtggaatccg ccgatggcga cgttcaccgc tgcaatattc gccgtaccat ccgttcgctg
    18721 gtgaccggcg accgtgtagt ctggcgtccg ggtaaaccgg cggcggaagg cgtaaatgtc
    18781 aaagggatcg tggaagcggt gcatgagcgt acctcggtgt tgacgcgtcc ggatttctac
    18841 gacggcgtaa aacctattgc cgccaacatc gaccagattg tcattgtctc cgccattttg
    18901 ccggagctgt cactcaatat tatcgaccgt tacctggtgg cctgcgaaac cttgcagatt
    18961 gagccgatta ttgtgctcaa caagatagac ctgctggacg acgaaggcat ggcgttcgtc
    19021 aacgagcaga tggatatcta ccgcaatatc ggttatcgcg tattgatggt ttccagccat
    19081 actcaggatg ggctaaaacc gctggaagag gcgttgaccg ggcgcatcag catttttgcc
    19141 gggcagtctg gcgtcggcaa atccagcctg ctgaatgcgt tgctggggct gcaaaaagag
    19201 atcctgacca acgatgtctc tgacaactcg gggctcggtc agcacaccac gaccgccgct
    19261 cggctgtatc acttcccgca cggcggtgat gtgattgatt cccctggtgt gcgtgagttc
    19321 ggcctctggc acctggagcc ggaacaaatc actcagggct ttgtcgaatt ccatgactat
    19381 ttaggtctgt gtaaatatcg cgattgcaaa cacgataccg atccgggctg cgctatccgg
    19441 gaagcggttg aggaagggaa aatcgcggaa acccgtttcg aaaactatca ccgtattctg
    19501 gaaagcatgg cgcaggtaaa aacgcgtaaa aacttttctg atacggatga ctgacaatta
    19561 agctaaccgt cgttagaatc gtcccctttt tttcaggatc cggcatgttg gccggatcag
    19621 gaacgacaaa acaatggcct ggaggctacc ttgttaaatt catttaaact ttcgctacag
    19681 tacattctgc cgaaactatg gcttactcgc ctggcgggtt ggggcgcaag caagcgggca
    19741 ggatggctga caaaactggt tatcgatctg ttcgttaaat actacaaggt cgacatgaaa
    19801 gaggcgcaaa agccggacac cgccagctac cgcaccttta acgaattctt tgtccgtccg
    19861 ctgcgtgacg aagtacgccc aatcgatacc gatccgaatg tactggtcat gcctgccgat
    19921 ggcgttatca gccagttggg taaaatcgaa gaagataaaa tcctgcaagc caaaggccac
    19981 aactacagcc tcgaagccct gctggcaggc aactatctga tggcggacct gttccgcaac
    20041 ggtacgtttg tgaccactta cctctccccg cgtgactacc atcgcgtaca catgccgtgc
    20101 aacggtattc tgcgtgagat aatctacgtg ccgggcgatc tcttctccgt taaccatctc
    20161 accgctcaga acgtgccgaa tctgtttgcc cgtaacgaac gcgtgatttg ccttttcgat
    20221 accgaatttg gcccaatggc gcagattctg gtcggagcga cgattgttgg cagcattgag
    20281 acggtctggg cgggcaccat tacgccgccg cgcgaaggta tcatcaagcg ctggacctgg
    20341 cctgccgggg aaaacgacgg ttctgtggca ctgctgaaag gccaggaaat gggtcgcttt
    20401 aaactcggtt ccaccgttat caacctgttt gcaccgggta aagtgaatct ggttgagcaa
    20461 ctggaaagcc tgtctgtcac gaaaattggc cagccgctgg cagtatctac cgaaaccttt
    20521 gttacgccag acgctgaacc cgccccactt cctgctgaag agatcgaagc agaacacgac
    20581 gccagcccat tggttgacga taaaaaagac caggtctaat ccatcaaagg aaacgctgac
    20641 gtgcgcctga ttatcacttt tctgatggcc tggtgcctca gttggggggc gtacgccgcg
    20701 acggcccccg atagcaaaca aatcactcag gaactggagc aggcaaaagc ggcgaaaccc
    20761 gcacagccgg aagtcgtaga ggcgctccag tctgccttaa atgcgcttga ggaacgaaaa
    20821 ggttcccttg agcgcatcaa acaatatcag caagttatcg ataattatcc gaaactctcc
    20881 gctactctgc gcgcacaatt aaacaacatg cgtgacgagc cgcgcagcgt gtcgccggga
    20941 atgtctaccg acgcgctgaa tcaggaaatt ctccaggtca gcagccagtt gctggataaa
    21001 agccgtcagg cccagcaaga gcaggagcgc acccgcgaga ttgccgattc gctgaatcaa
    21061 ctgccgcaac agcaaaccga cgcccgccgt cagttaaatg agatcgagcg ccgcctggga
    21121 acgcttaccg gcaatactcc gctcaatcag gcacaaaatt tcgcgttgca gtctgactct
    21181 gcacgtctta aggcgctcgt tgatgaactg gagctggcgc agctgtctgc caataaccgc
    21241 caggaattag cgcgcttacg ctcagagctg gcggaaaaag agagccagca actggatgcg
    21301 tatttgcagg ccttgcgtaa tcaattaaac agccaacgtc agcttgaggc ggagcgggcg
    21361 ctggaaagta ccgaattgct ggcagaaaac agcgccgatt tgccgaaaga tatcgtcgcg
    21421 caattcaaaa ttaaccgcga actatcggcg gctttgaatc aacaggcgca gcggatggat
    21481 ctcgttgcct cgcaacagcg tcaggctgcc agccagacgt tacaggtccg gcaggcgttg
    21541 aatacgctgc gtgaacagtc gcaatggctg ggatcgtcca atctgctcgg cgaagcgctg
    21601 cgggcgcagg tggcacggct gccagaaatg ccaaaaccac aacagcttga taccgaaatg
    21661 gcgcagttgc gtgtgcaacg gttacgttat gaggatctgc ttaataaaca gccgctgcta
    21721 cggcaaatcc atcaggccga cggtcagccg ctgaccgccg agcaaaaccg tattctggaa
    21781 gcacaactgc gcactcagcg tgagttgctg aactcattgt tgcagggtgg cgacacgcta
    21841 ctattggaac tgaccaagct gaaagtctcc aacgggcaac tggaggatgc gctgaaagag
    21901 gtgaacgaag caacgcaccg ctatctgttc tggacctctg acgtgcgccc gatgaccatc
    21961 gcctggccgc tggaaatcgc ccaggatctg cgtcgtctca tttcgctgga caccttcagt
    22021 cagttgggca aagccagtgt gatgatgctg accagcaaag agacaatttt gccgctgttt
    22081 ggcgcgttga ttctggtcgg ttgcagtatt tactcgcgcc gctatttcac ccgttttctt
    22141 gaacgttcgg cggcgaaagt tggcaaagtg actcaggatc acttctggct gacgttgcgc
    22201 actcttttct ggtcgattct cgtcgcgtca ccgttaccgg tgctgtggat gacgctgggt
    22261 tacggcttgc gcgaggcgtg gccttatccg ctggcggtcg cgattggcga tggtgtaacg
    22321 gctaccgtgc cgctgctgtg ggtagtgatg atttgcgcca cctttgcccg cccgaacggc
    22381 ttgtttatcg ctcattttgg ctggccgcgc gaacgtgttt cccgtgggat gcgctactac
    22441 ctgatgagca tcgggcttat tgtgccgctg attatggcgc tgatgatgtt cgataacctc
    22501 gacgaccgtg aattctccgg ttcgctggga cggctttgct ttatcctcat ttgcggtgcg
    22561 ctggcggtgg tcaccctcag cctgaaaaag gccgggatcc cactgtatct caacaaagag
    22621 ggcagcggcg acaacattac caaccatatg ctgtggaaca tgatgattgg cgcgccgttg
    22681 gttgccattc tggcgtcggc tgtgggttat ctggcaacgg cacaggcgct gttagcgagg
    22741 cttgaaacct cggttgccat ctggttcctg ctactggtgg tttatcacgt tatccgccgc
    22801 tggatgctga tccagcgccg caggctggcg tttgaccggg cgaagcatcg ccgggcagag
    22861 atgttagcgc aacgtgcgcg tggcgaagag gaagcgcatc atcacagtag cccggaagga
    22921 gcaattgaag tcgatgaaag cgaagtcgat ctcgatgcca tcagtgcgca atccttgcgg
    22981 ctggtgcgct caattttgat gttgatcgcc ctgctttctg tcattgtgct gtggtcagaa
    23041 atccattccg ctttcggctt cctcgaaaat atttcgctgt gggatgtcac ctccacggta
    23101 caaggcgtag aaagtctgga gccaattacc ctcggtgcgg tgctgattgc cattctggtg
    23161 tttatcatca ccacgcagct ggtgcgcaac ctgcccgcgc tgctggaact ggcgattttg
    23221 cagcacctgg atttaacgcc gggtacaggc tacgccatca ccaccatcac caaatatctg
    23281 ctgatgctga ttggcgggct ggtcggcttc tcgatgattg gtattgagtg gtcgaaattg
    23341 cagtggctgg tcgccgcgct cggtgttggt ctcggttttg gtttgcagga aattttcgcc
    23401 aactttatct ctggtctgat tatcctgttc gaaaaaccga ttcgcattgg cgatacggtg
    23461 acaattcgcg atctcaccgg tagcgtgacg aaaattaaca cccgcgccac caccatcagc
    23521 gactgggacc gtaaagagat aatcgtgccg aacaaggcgt ttattaccga gcagtttatc
    23581 aactggtcgc tctctgactc ggttacgcgc gtagtgttga cgattccggc ccctgctgat
    23641 gccaacagtg aagaagtgac ggaaatcctg ctcaccgcag cgcgtcgctg ctcgctggtg
    23701 atcgacaacc cggcaccgga agtcttcctg gtggatctgc aacaagggat tcagattttc
    23761 gagctgcgta tttatgccgc tgagatgggt caccgtatgc cgctacgcca tgagatccac
    23821 cagctgattc tggctggctt ccatgcccac ggtatcgata tgccattccc gcccttccag
    23881 atgcgtctgg aaagcctcaa cggtaaacaa acggggagaa cgctgacgtc tgcgggcaaa
    23941 ggtcgtcagg cgggaagttt gtaatgcatg aagctgccaa atgatgtcaa cgtggctcat
    24001 ttagcagctt aacatcatct tcagttatca ccggccctgg agagtatcat ccatacgcat
    24061 tacttcctga caactcatcg ccggagagac gatgagttgc aggatttcat atgatcagtc
    24121 gagagctaac tcaggtttat tcgccatgcg actgcaatag ttctggtaaa tcgccatcgc
    24181 taacagcgag aagaagacag ggccgccaat catccacaat gtgctgtccc agtctccggc
    24241 ttcaaccaca ggcggaataa tggtgaagac gttcgcaaat gtcaccacca gtacgacaac
    24301 caccgtcgca atcattgccg acatacgcgt tttgaaaatc acaaatggtc tgtcgagatc
    24361 ctgacgtgct ttaaagaatg ggaaagccag cgcgaggaac aggtaaggaa gcgtcggtaa
    24421 tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc tt
//
LOCUS       DAJTMK010000047        24172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTMK010000047 DAJTMK010000000
VERSION     DAJTMK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24172
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.048"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(133..1143)
                     /locus_tag="OO135_001621"
     CDS             complement(133..1143)
                     /locus_tag="OO135_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5397516.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVASFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(1176..2000)
                     /locus_tag="OO135_001622"
     CDS             complement(1176..2000)
                     /locus_tag="OO135_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5397517.1"
                     /translation="MLAVDQREAMRLMFAAAGQPKPIADSVLTDFKVAATRILSPYAS
                     AVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNGIPVDSVEIDMSVDPHKAREMG
                     AKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVSIIEPVVRPPRRGWDFDRESAI
                     VAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQLNDQMKMPWVILSSGVDADIFG
                     RAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLCDVSVPRLQRLAEIVDEGIAQR"
     gene            complement(2088..2966)
                     /gene="yihU"
                     /locus_tag="OO135_001623"
     CDS             complement(2088..2966)
                     /gene="yihU"
                     /locus_tag="OO135_001623"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5397518.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            3139..4035
                     /locus_tag="OO135_001624"
     CDS             3139..4035
                     /locus_tag="OO135_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5397519.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            4075..4863
                     /locus_tag="OO135_001625"
     CDS             4075..4863
                     /locus_tag="OO135_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5397520.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(4969..6792)
                     /gene="typA"
                     /locus_tag="OO135_001626"
     CDS             complement(4969..6792)
                     /gene="typA"
                     /locus_tag="OO135_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5397521.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYSSALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
     gene            7165..8574
                     /gene="glnA"
                     /locus_tag="OO135_001627"
     CDS             7165..8574
                     /gene="glnA"
                     /locus_tag="OO135_001627"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5397522.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            8748..9797
                     /gene="glnL"
                     /locus_tag="OO135_001628"
     CDS             8748..9797
                     /gene="glnL"
                     /locus_tag="OO135_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5397523.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGRESGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            9809..11218
                     /gene="glnG"
                     /locus_tag="OO135_001629"
     CDS             9809..11218
                     /gene="glnG"
                     /locus_tag="OO135_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5397524.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            11329..11439
                     /locus_tag="OO135_001630"
     CDS             11329..11439
                     /locus_tag="OO135_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5397525.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(11629..13002)
                     /gene="hemN"
                     /locus_tag="OO135_001631"
     CDS             complement(11629..13002)
                     /gene="hemN"
                     /locus_tag="OO135_001631"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5397526.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VECYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(13191..13700)
                     /gene="yihI"
                     /locus_tag="OO135_001632"
     CDS             complement(13191..13700)
                     /gene="yihI"
                     /locus_tag="OO135_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5397527.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            14282..14914
                     /locus_tag="OO135_001633"
     CDS             14282..14914
                     /locus_tag="OO135_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5397528.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(15296..18082)
                     /gene="polA"
                     /locus_tag="OO135_001634"
     CDS             complement(15296..18082)
                     /gene="polA"
                     /locus_tag="OO135_001634"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5397529.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            18446..19378
                     /locus_tag="OO135_001635"
     CDS             18446..19378
                     /locus_tag="OO135_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5397530.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MAINVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(19419..20849)
                     /locus_tag="OO135_001636"
     CDS             complement(19419..20849)
                     /locus_tag="OO135_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5397531.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIYPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(21004..21630)
                     /gene="dsbA"
                     /locus_tag="OO135_001637"
     CDS             complement(21004..21630)
                     /gene="dsbA"
                     /locus_tag="OO135_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5397532.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(21647..22633)
                     /gene="srkA"
                     /locus_tag="OO135_001638"
     CDS             complement(21647..22633)
                     /gene="srkA"
                     /locus_tag="OO135_001638"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5397533.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDLRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(22710..22979)
                     /locus_tag="OO135_001639"
     CDS             complement(22710..22979)
                     /locus_tag="OO135_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5397534.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            23049..23633
                     /gene="mobA"
                     /locus_tag="OO135_001640"
     CDS             23049..23633
                     /gene="mobA"
                     /locus_tag="OO135_001640"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5397535.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            23615..24142
                     /gene="mobB"
                     /locus_tag="OO135_001641"
     CDS             23615..24142
                     /gene="mobB"
                     /locus_tag="OO135_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5397536.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5915 a         6143 c         5893 g         6221 t
ORIGIN      
        1 aatatcaatt acgtcctgtg gccgactaat tgaataagtt gcaggactgg tgatttgact
       61 catcgcagtg tcctttttaa ttgttgtagg gtggcgctga cgtgacgcag cgccagacag
      121 agatttagaa aatcattccg gcgcaaattg cagcatgacc tttatggagt cattttgctg
      181 cgcgcgttca aatgccgccg ctgcatcctc aattgcaaat aaatcggtaa ccagttcact
      241 ggtatcaaac cgcccatccg ccagccattc gataacagca tcgaaatctt cagggatgta
      301 catgccagag ttgagcaagt cacgctcgaa acgttgcatc atcggtaatg gtatttcacg
      361 cggcccactt ggtacgccca tgcacacgac ggtgcctccg gcatatacac gggtacatgc
      421 atcgttaagc gtggcctgcg ctgcaacgac atcaatcgcc cccgtaaatc gcacatctgc
      481 ggccagctca cctggtgccc aaacttctgc tgcgcccagc tttaacgcca gcgcgcgttt
      541 ggcagcgtca ggttcaatca cggtaatctt acctgccccc ataatgcgca gtgcctgcac
      601 gatggataag cctatcgttc cggcaccaat taccagtacg ctttccagag aagctttcgg
      661 cattcggtta acgcagtgac gggcacaagc cgccggttcg gcaaaggcca acacttttag
      721 cggtaaagag gctggtgcga catggcaatt acgcgcagga acaatgtgtt gtgagcgagc
      781 aaagcccggt gcgcgaaaac tagcaacctg cggtggttca caaagattaa aacgtcctgc
      841 tttgcaggct cggcattcca tgcaagccat gatgggatcg accacaacat gatcgcccgg
      901 ctgtacattt ttaacgtcgc tgccaacttc cgtaacgcgc gctgcaattt catgaccggg
      961 gacgacaggt ggtttggcaa acggatgacc gcctttcaga acatgcagat ccgaaccgca
     1021 tatcccatag aacacaggcg caatccgcac ttcgtgcggt ttaagttcct ggtgtggtac
     1081 ttcaacatat tgagtggtga ctttcccaat atctgaaaat aaaacctggg taattttatc
     1141 cattgttatt ctccagaatc gatatccata ggggattagc gttgcgcgat accttcatcg
     1201 acaatttctg ccaaccgttg taaacgagga acagagacat cgcacagcat agtttgtgga
     1261 tcttgtgcac caacaaccga cgcccaaacc gctcgtccag ccaggaagcc cgacgcacca
     1321 cctttcatgg caatacttac tgcacggcca aaaatatcgg catcaacgcc agaagaaaga
     1381 attacccacg gcatcttcat ctgatcgtta agttgctgac aggcggcaag taaggttttt
     1441 tcatcgcctt tgccccctaa tggcatctcg gctttgtaaa gatcagcctc tgttccacca
     1501 agctctgcgg cggcggcaac aatggcgctt tcacgatcaa agtcccaacc gcgacgaggt
     1561 ggacgcacca caggttcaat aatgctcacc agcccggcac tacggcagcg acgaacaaat
     1621 ttatcaacca ttgccagacg ttcctctgcg ggttcatctt cacgccagag tacaagtaat
     1681 ttcattgcct tagcccccat ttcacgggct ttatgcgggt cgacggacat atctatttca
     1741 acgctatcaa ccgggatgcc attacctggg ataaaaagat cggcagcgac aatcaggccg
     1801 caagaattag caacagcgcc ctgctcgact atttgctcaa gacaaaattg tttatccgcc
     1861 agaacggcag aagcataagg agaaaggatt cgggtcgcgg caactttaaa atcggtgagt
     1921 acactgtcgg cgatgggttt tggctgccct gctgccgcaa acatcaagcg catggcctca
     1981 cgctgatcga cagctaacat ggcaaatcca ccacctgggc gggagatatc tttcagcgta
     2041 aatggggtga agtgggatta catagaaact cctgttaata gataaaatta aagaccagcc
     2101 tgctggcgaa cctgatttag cagggcactc cagtcctgcc gccctttgcc gtcttgactt
     2161 gctgcagcgt aataactttc tgctgcaatc cccatattga gcggtgcccc cgtctcccga
     2221 gcgacgtcaa ctgcaatacg cagatctttc aacgccagat ccaccatgaa ggcgggtgaa
     2281 agatcgcctt tcagcacttt gccaggccag gtggtcgtaa aatgtccttt ccctgctgcc
     2341 gtaccactca tcaccttaat tgcgacatca agatttaatc ccagtgactc acataacaca
     2401 gcggcctcgg aggacagcgc attaagcgca atgctcatat agttgttaat tagcttcacg
     2461 cggatcccca ttcctgggcc gccagcctcg accagctcat tacccatgca cataagaatc
     2521 gggcgtgcac gtgcaatctg ctcatcagtg ccacccgcta aaatcagcaa tgtgccatca
     2581 atcgcattca cggacgttct gcccacggga gcgtccatca tactgatgcc tttttcttgt
     2641 aactcccgta ttaatgcatc ggtctgtaag ggatgaatag tcgacatatc aatcaataac
     2701 gcgtcagatg aaacagcctc acagacgcct ttttccccca acagaacctg tcgaacaatg
     2761 tcaccattgg gaagcatagt gatgataaat tcagcgcctg ctgctgcttc cgctggggtt
     2821 tgtgccgcag tggccccctg cgtaactagc gcatcgacgg cctgtgcatt gacatcaaat
     2881 acctgcaatg aatgcccttt ttgcaataga ttgttagcca ttgggctacc catctgcccc
     2941 agcccgagaa atgcaatggt tgccatgaat actctcctgt ctgacattga caaaataatg
     3001 acaattgaaa attcgttttc gtctgttttt gagcatattt gtaatttatg ttcaaaaaca
     3061 cgacacgcat cactaatttg atcatttgcg tcatttaaat tgaccaaaga cgacaaaatt
     3121 aaataggaca agacaaatat gaccagaatt gcatgcgtgg gtatcacagt tcaggacaga
     3181 atttattctt tgcccactct gccggagggc ggcggaaaat atcaggcaaa tcattatctt
     3241 gagattggtg gcggcccggc agcaacagcc gctgttgcaa tcgcaaagct tggcgtggag
     3301 gttgatttca tcggtcgcgt cggcgatgac agttgtggaa ataccttact tgcagaactt
     3361 gagggttggg gcgtcaatac cgcattttgc cgccgatatc ctaacgcccg ttcgtcgcaa
     3421 tcagcgatcc tcgttgatca gcatggcgaa cggattatcg ttaattatcc aagtcctgat
     3481 ctcggtactg atgccgaatg gttagaagcg atcgatttct ctcgttatga tctgattctg
     3541 gccgatgttc gctggcatag cggtactgaa aaagcattct cgctagcgcg ccttgcgggt
     3601 gtgacaacgc ttttagacgc cgatatgaca ccgcaggata tttctccact tgtcgcgctt
     3661 gccgatcacg ccgtgttctc aacaccaggg ctgaaacgca tgactggcct gcagtcacct
     3721 gaagaaggtt tgttccaggc aacgacacaa acagcaggta aagtgtatgt tacgctgggt
     3781 tctgaaggat cgttgtggat tgaagatggt catttgtgtc aacaggaggc attttctgtc
     3841 aatgtggttg ataccactgg agcaggcgat gtttttcacg gcgcactcgc ggtagcgttg
     3901 gcagaaaaaa tgccaacaaa agaggctatt cgttttgcca gtgcggttgc cgcgatgaaa
     3961 tgtacccagc ctggcggtcg ggccggcatt cctaatcgtg agcaaaccga atcatttttg
     4021 tcactttatg cgtaaaatgg taaacatcta ccgaggaggg gcaagggagt attcatgagt
     4081 atcatcgaag tgacaggtaa tccacgccat gaccaactcg ttcatctaat cgcagaacgc
     4141 ggttatatga atattgaaga actggcacaa ctgctggatg tttcaacgca aaccgtgcgc
     4201 cgggatatcc gaaaactcag tgagcagggc ttgatcaccc gtcatcatgg aggggccggg
     4261 cgcgtctcca gcgtcatgaa taccgctttt gagcaacggg aactttcgct caccgccgag
     4321 aaacgggcga tcgctgaggc agtcgccgat taccttcccg aacgctgtac cgtctttatc
     4381 accatcggta caaccgtaga agccgttgcc agggcattac tcaaccggcg tgatttacgc
     4441 attatcacta acagcctacg tgtggcacag attctttata agaaccagga tattgaagtg
     4501 atggtgccgg gaggcacttt acgcgctcat aacggcggga ttatcggccc aggagccgtg
     4561 gattttattg aaggcttccg cgcagattat ttaatcacca gtataggtgc cattgaacat
     4621 gacggcaccc tactggaatt tgatgtaaat gaagcgttag tcgcgagaac gatgattaaa
     4681 catgcgcgga atacattgtt agtggccgat catacgaagt ttgccgcgtc tgctgccgtt
     4741 tcaattggca atgcacggaa tgtcagggct ttttttactg atgccccgcc tcccaattct
     4801 ttctgccagt tgttaagtga agagaatgtt gaactggtgg ttgccgagca agaagtatcc
     4861 tgaaattgaa atttcggaaa atccgctggg aatttcagcg gattttgttt tccataagca
     4921 aaaaaaacct gccagcaatg gcaggttttt tataactaaa aaacgaaatt aatagtcttt
     4981 cggtgcgcgg ttggcgcgac gacgatcgtt ttccgtcagg tgacgtttac gaatacggat
     5041 agaggtcgga gtcacttcta ccagttcgtc atcatcgatg aactccagag cttgttccag
     5101 agtcatgcgg ataggcggaa ccagaacaac ggcttcgtca gtaccggaag cacgcatgtt
     5161 ggtcagtttc ttaccggtca ggcagtttac agtcaggtcg ttagagcggc tatgaatacc
     5221 gataatctga ccttcgtaaa cttctgcacc gtgaccgagg aacagcttac cgcgatcctg
     5281 cagaccgaac agcgcgaacg cgaccgcttt accctgaccg ttagagatca gtacgccgtt
     5341 ctgacgctga cccacttcac ccggacgtac gtcgtcgtag tggctgaagg tggagtacag
     5401 cagaccagta ccggaagtca tggtcatgaa ctcagaacgg aagccaatca gaccacggct
     5461 tgggatcacg tagtcgagac gtacgcggcc tttaccgtct ggattcatgt ttttcaggtc
     5521 gcctttacgt tcgcccagcg cctgcattac agaaccctga tgctgttctt caacgtccag
     5581 agtcacgttt tcatatggct cttgtttacg accgtcgatt tcacggaaga taactttcgg
     5641 acgggatacc gccagttcga aaccttcacg acgcatgttt tcgatcagaa cagacaggtg
     5701 tagttcgcca cgaccagaaa cgcggaacgc atcagcgtct tcggtttctt ctacgcgcag
     5761 cgcaacgttg tgtaccagtt ctttgttcag acgatccagg atctgacgag acgttacgaa
     5821 cttaccttct ttaccgcaga acggcgaggt gttaacgcag aagaacatag aaacggtcgg
     5881 ctcatcaacg gagagtgccg gcagcgcttc aacgttttgc gtgtcgcaaa cggtgtcaga
     5941 aatgttcagt tcgccaaggc ccgtgatcgc aacgatatcg ccagcttccg ccaggtcggt
     6001 ttcgatacgt tccagaccga ggtggcccag cactttaccg actttcgcgt tgcgggtttt
     6061 gccttcgcta tcgatgatag tgacctgctg gttcggcttc actttaccgc gcttgatgcg
     6121 gccaatgccg ataacgccaa catagctgtt gtaatcgagc tgagaaatct gcatctggaa
     6181 cggaccgtca aggtcaacgt ccggcgcagg aacgtggtca acaatcgcct ggtacagcgg
     6241 ggtcatgtct tccgccatat cttcgtggtc cagacccgcg ataccgttca gcgcagaaga
     6301 gtaaacgatc gggaagtcca gctgctcgtc ggtcgcgtcg aggttaacga acagatcgaa
     6361 tacctgatcc acaacccaat caggacgcgc gccagggcgg tcaactttgt tgataacaac
     6421 aatcggcttc aggccgtaag caaacgcttt tttggttacg aagcgcgttt gcggcatcgg
     6481 gccgtcaaat gcgtcaacca ccagcagcac tgagtctacc atggacatta cacgttcaac
     6541 ttcaccaccg aagtcggcgt gccccggggt atcaactatg ttgatacggt aatcattcca
     6601 tttgatagcg gtgtttttcg cgaggatggt aatcccacgc tctttctcca aatcgttgga
     6661 gtccatcacg cgctcttggg tttcggcacg agagtcgaac gtaccggatt gttggagcag
     6721 cttgtctacc agggtggttt taccatggtc tacgtgcgcg atgatggcga tattacgcaa
     6781 tttttcgatc acaactttgc ctcaggcatt agaaatagcg cgttattgta cacggattaa
     6841 tcgcactaca aaacaggatc acaaacatcc tccgcaaaca agtattgcag agtccctttg
     6901 tgatcgcttt cacggagcat aaaaagggtt atccaaaggt cattgcacca acatggtgct
     6961 taatgtttcc attgaagcac tatattggtg caacattcac atcgtggtgc agcccttttg
     7021 cacgatggtg cgcatgataa cgccttttag gggcaattta aaagttggca cagatttcgc
     7081 tttatctttt ttacggcgac acggccaaaa taattgcaga tttcgttacc acgacgacca
     7141 tgaccaatcc aggagagtta aagtatgtcc gctgaacacg tactgacgat gctgaacgag
     7201 cacgaagtga agtttgttga tttgcgcttc accgatacta aaggtaaaga acagcacgtc
     7261 actatccctg ctcatcaggt gaatgctgaa ttcttcgaag aaggcaaaat gtttgacggc
     7321 tcctcgattg gcggctggaa aggcattaac gagtccgaca tggtgctgat gccagacgca
     7381 tccaccgcag tgattgaccc gttcttcgcc gactccaccc tgattatccg ttgcgacatc
     7441 cttgaacctg gcaccctgca aggctatgac cgtgacccgc gctccattgc gaagcgcgcc
     7501 gaagattacc tgcgttccac tggcattgcc gacaccgtac tgttcgggcc agaacctgaa
     7561 ttcttcctgt tcgatgacat ccgtttcgga tcatctatct ccggttccca cgttgctatc
     7621 gacgatatcg aaggcgcatg gaactcctcc acccaatacg aaggtggtaa caaaggtcac
     7681 cgtccggcag tgaaaggcgg ttacttcccg gttccaccgg tagactcggc tcaggatatt
     7741 cgttctgaaa tgtgtctggt gatggaacag atgggtctgg tggttgaagc ccatcaccac
     7801 gaagtagcga ctgctggtca gaacgaagtg gctacccgct tcaataccat gaccaaaaaa
     7861 gctgacgaaa ttcagatcta caaatatgtt gtgcacaacg tagcgcaccg cttcggtaaa
     7921 accgcgacct ttatgccaaa accgatgttc ggtgataacg gctccggtat gcactgccac
     7981 atgtctctgt ctaaaaacgg cgttaacctg ttcgcaggcg acaaatacgc aggtctgtct
     8041 gagcaggcgc tgtactacat tggcggcgta atcaaacacg ctaaagcgat taacgccctg
     8101 gcaaacccga ccaccaactc ttataagcgt ctggtcccgg gctatgaagc accggtaatg
     8161 ctggcttact ctgcgcgtaa ccgttctgcg tctatccgta ttccggtggt ttcttctccg
     8221 aaagcacgtc gtatcgaagt acgtttcccg gatccggcag ctaacccgta cctgtgcttt
     8281 gctgccctgc tgatggccgg tcttgatggt atcaagaaca agatccatcc gggtgaagcc
     8341 atggacaaaa acctgtatga cctgccgcca gaagaagcga aagagatccc acaggttgca
     8401 ggctctctgg aagaagcact gaacgaactg gatctggacc gcgagttcct gaaagccggt
     8461 ggcgtgttca ctgacgaagc aattgatgcg tacatcgctc tgcgtcgcga agaagatgac
     8521 cgcgtgcgta tgactccgca tccggtagag tttgagctgt actacagcgt ctaatagttg
     8581 aagttgtact acccggcgca acaacgccgg gatttagttg ccgtggaaac tttcagccca
     8641 tctctgcatg ggcttttttc tccgtcaatt ctctgatgct tcgcgctttt tatccgtaaa
     8701 aagctataat gcactaaaat ggtgcaacct gttcaggaga ctgctttatg gcaacaggca
     8761 cgcagcccga tgctgggcag atcctcaact cgctgattaa cagtattttg ttaatcgatg
     8821 acaacctgga gatccattac gccaaccctg ccgcgcaaca actgctcgcc caaagctccc
     8881 gcaaattgtt tggtacaccg ttaccggaac tgttgagcta cttctcatta aatatcgagc
     8941 tgatgcaaga aagtctggag gcggggcaag gttttaccga taacgaagtg acgctggtca
     9001 tcgacgggcg ctcgcatatc ctttctgtga ctgcccagcg tatgccggac ggcatgatcc
     9061 tgctggagat ggctccgatg gataaccagc gccgcttaag tcaggaacag ctacagcacg
     9121 cccagcaggt tgctgcccgt gatttagtgc gcggcctggc acatgagatt aaaaatccgc
     9181 ttggcggttt acgtggcgcg gcgcagctgc tcagcaaagc gttacctgac ccatcactac
     9241 tcgaatatac caaagtgatt atcgaacagg cggaccggct gcgaaatctg gtcgaccgtc
     9301 tgttggggcc gcagctgccc ggtacgcgcg ttaccgaaag tattcacaaa gtggctgaac
     9361 gcgtggtaac gctggtgtcg atggaactgc cggacaacgt gcggttgatt cgtgattacg
     9421 atcccagcct gccggaactg gcgcacgacc cggatcaaat tgaacaggtc ttgctgaata
     9481 ttgtgcgcaa tgcgctacag gcgctggggc cggaaggcgg tgaaatcatt ctgcgtaccc
     9541 gcaccgcgtt tcaactgacc ttacacggcg agcgctaccg gctggcggcg cggattgatg
     9601 tggaagataa cgggccgggc attccgcctc atttgcagga tacgctgttt tacccgatgg
     9661 tcagcggccg cgaaagtggc accgggcttg gcttatccat cgcccgtaat ttgattgatc
     9721 agcattcagg caaaattgaa tttaccagtt ggccaggtca taccgagttc tcggtttacc
     9781 tgcctatcag gaaataaagg tgacgtttat gcaacgaggg atagtctggg tagtcgatga
     9841 cgatagttcc atccgttggg tgcttgaacg tgcgctcgct ggagcgggtt taacctgtac
     9901 gacatttgag aacggcgcgg aagtactgga ggcgctggcg agcaaaacgc cggatgtgct
     9961 gctttcagat atccgtatgc cgggaatgga cgggctggcg ctgctcaagc agattaaaca
    10021 gcgccatcca atgcttccgg tcatcattat gaccgcacat tccgatctgg atgctgccgt
    10081 cagcgcctat caacaagggg cgtttgatta tctgcccaaa ccgtttgata tcgacgaagc
    10141 cgtggcgctg gttgagcgcg ctatcagtca ttaccaggaa cagcagcagc cgcgtaatat
    10201 tcagcttaac ggcccaacga ccgatatcat cggcgaagcg ccagccatgc aggacgtgtt
    10261 ccgtattatc ggtcggcttt cgcgttcttc tattagcgtg ctgattaacg gcgaatccgg
    10321 caccggtaaa gaactggtcg ctcatgccct gcatcgccac agtccgcgcg ccaaagcgcc
    10381 gtttatcgcg ctgaatatgg cagctatccc aaaagatttg atcgaatcag aactgtttgg
    10441 ccacgagaaa ggcgcgttta ctggcgcgaa taccattcgt caggggcgtt ttgaacaggc
    10501 cgatggcggt acattattcc tcgacgaaat tggtgatatg ccgctggatg tgcagacgcg
    10561 tttgctgcgc gtgctggcag acggtcagtt ttaccgcgtt ggcggctatg cgccggtgaa
    10621 agtggatgtg cggattatcg ctgccactca ccagaatctc gaacagcgag tgcaggaagg
    10681 taagttccgt gaggatctgt tccaccgcct aaacgttatc cgcgttcatc tgccgccgct
    10741 gcgcgaacgt cgggaagata ttccccgtct ggcgcgccat tttttacagg ttgccgcgcg
    10801 cgaactgggc gtagaagcga agttactgca tccggaaacc gaagctgctc tgacgcgtct
    10861 ggcgtggcca ggcaacgtgc gccagctgga aaacacctgc cgctggctaa cggtgatggc
    10921 cgccgggcag gaagtgttga ttcaggattt gcccggcgaa ctgtttgaat caacagttgc
    10981 ggagagtact tcgcaaatgc aaccggacag ctgggcgacg cttcttgcgc agtgggcaga
    11041 cagagcgctg cgttccggtc atcaaaatct gctttccgaa gcgcagccag agctagagcg
    11101 gacgttactg acgaccgcgt tgcgacatac gcaggggcat aaacaggaag cggcgcggct
    11161 actcggctgg ggccgcaaca ccctgacgcg taagttaaaa gagctgggga tggagtgatt
    11221 cacagcttgt gtgtaaagat tgattattga gcgcaaattg ctggtatttt acgctttact
    11281 gttccgataa gttcagtatg atctgcccgg aaaacgggga gagtcattat gctggaatca
    11341 ataattaatc tggtatcgag tggcgcagtt gacagccaca caccgcaaac tgccgttgct
    11401 gccgtgctgt gtgccgcaat gattgggctg tttagttgag tttcagattg ctgatgaagt
    11461 gctctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcaatattt
    11521 tcaacaggtt ttgtaggcct gataagcgta gcgcatcagg caatcttgtt ctcagcacat
    11581 tgatggcgct ttcgttttct actttgtaaa cgaagcgcca ttcactattc aaatcacccg
    11641 tgagaactgc tgcatccgcg ctttctggcg cagataggta tcaaagcaca tgcaaatgtt
    11701 gcggatcagc aagcgacctt tcgccgtcac ctgtattccc ttctcatcca catccaccag
    11761 cccatctttt gctaacgggg cgagcagctt gagatcttcc gcaaagtaat cagcgaagtg
    11821 caaatcccac tgtttctcaa taggggcgta atccagacgg aagttgcaga tgagcgactt
    11881 aatcacatcg cggcgaatac agtcatcacg cgttagcgca ataccacgcc acagcgcatt
    11941 gccttgttca tccacttgct gatagtactg cttcaactct ttctggttct gcgcgtagca
    12001 gtcgccaatc atgctgatgg cggaaacgcc catccccagc agatcggtat cgccctgagt
    12061 ggtgtagccc tggaagttac gatgcagcac gccttcacgc tgggccaccg ccagctcgtc
    12121 atccggacgg gcaaagtgat ccataccgat aaactgatag cccgattgcg tcaggaaggc
    12181 gatggtttcc tgcaggatat cgagtttttg ctgcggactc ggcaggtcag catctttgat
    12241 tttgcgctga gcagcaaaaa tggtcggcag atgcgcgtag ttaaagacac tcagacgatc
    12301 ggggttcagc tccgccacac gtttcagggt aaaggcgaaa ctctccggcg tctgtttcgg
    12361 caggccgtaa atcaggtcga tgttggtgga ggtaaaacca atctcacgcg catggttaag
    12421 cagtgcaaag atgaactctt catcctgctc gcggttaacc agacgttgca cttctttgtt
    12481 gaagtcctgc acgcccatgc tcaggcgatt aaagccttcg gcgcgtaaat gatcgagtac
    12541 atccagttcg atttcccgcg gatcgacttc gatcgaaatc tccgcatcgg cattgaactg
    12601 gaagttttcg cgcagcagct tcatcaggcg gctgatttgc gctttattca gatacgtcgg
    12661 cgttccgccg ccccagtgca attggctgac gtgacgcccg gcgaacagcg gtgcacgatg
    12721 gacgatttct tgctccagcg cgtccagata ctgatcggcc ttgtgctgct ggcgagtaac
    12781 aatcttattg caaccgcaga agtaacaaag cttatggcag aacgggatat gtacgtagag
    12841 agataatgga cgctcaggat agcactccac ggcttgtaaa aacgcctgtt cgccgaagtc
    12901 ttctgaaaac tccagcgcgg tcgggtacga ggtgtatcgt ggcccggaat agttatattt
    12961 ctggatcagg gccagatccc agtcgatttg ctgtacagac atgctcactc cttccgatgg
    13021 cgtctctggc ggctacggcg taccggcgga tgcccgtttc gggtcatcag ccggttgcgc
    13081 agccactttt gtcgccgcga caaccgctgt agtttaacga ataaccacac cagataacat
    13141 ataaccggaa gggttatcag cagaacggga aggcccacgg cggcaatccg ttagttgccc
    13201 cgcagcagac gcatcatgtc ttcttgcttc tcgtcttctt cctcttcttc gtcatcgtca
    13261 taagagaggc cgagtttctg catcaactca tcaatacgat ccagtttagc atccacccag
    13321 gattgctctt cggcactcag ggtttcgcct gcttccagac gttccagcag cgcatccaga
    13381 cgctcatccg tttccagtaa ttccaactcc gcctgcggtg aaagcatagg tttctcactc
    13441 ttcggtttgt gctgtttggt gactttttca gtcacgccca atggaatagg ggttttactg
    13501 ccaatacgtg gatcttttgg tgcgttctgg cctttgctgc ctgacgtggt gtttccgccc
    13561 gctgcgcggc tgcccggcgc atgaccacga cgttttttct gacgcttgcg atcacgagct
    13621 tcctgatcca gctcctcgcg tgtttttcga cgcgcttttg catgaccttt gctgcgtgag
    13681 tttgaagatg atggtttcat aatggttcgt cttttagccg ttttattcag tatcgatttg
    13741 cggcggaatc taacagaaag caagcaaaga aaaaaggcga cagattactc tgtcgccttt
    13801 tttcctgact cataaccctt aacgggtctt acaatccttg caggccgcca acacaccctg
    13861 tttttccttt agccggaaca atccgtgttg attccatttc cgtttaatta cgtctccgga
    13921 cgtttgtctt cctgacaatc ctctgtcttc gcctcctggc gctcctgacc ctcatcctga
    13981 gtcattgttc ctgttagcgt cctcgctcta tgggtgctac tttacgcctt tgctttaaac
    14041 aaacaatcaa ccgccatcag tatttgcaca ttttcagagt tttatataaa agcaattcac
    14101 tgatttatat agcttatttg acatgcatcg cagaattttc tctgctattt cgcataaaga
    14161 ctatcggcaa tgtctcacaa agcgtatggc ttttacttac aacgctcacg ccgaaaaatc
    14221 cgccttttat tatggttcag gtataatcgc cacaaagctt tgactgacgg agacgaccgc
    14281 tttgactaat ttgaattatc aacagacgca ttttgtgatg agtgcgcctg atattcgcca
    14341 cctaccttcc gataccggaa ttgaagtggc ttttgcaggc cgttccaacg caggtaaatc
    14401 cagcgcgctg aacacgctga ctaaccagaa aagcctggct cgtacctcaa aaaccccagg
    14461 gcgcacccag cttatcaacc tgtttgaagt ggctgacggc aagcgtctgg ttgacttgcc
    14521 tgggtacggt tatgcggaag tcccggaaga gatgaagcgc aaatggcagc gtgcgctcgg
    14581 cgaatacctc gaaaaacgtc agagcctgca aggtctggtg gtgctaatgg atattcgcca
    14641 tccgctgaaa gatttggatc agcagatgat tgagtgggcg gtagacagca atatcgccgt
    14701 tctggtgctg ctgaccaaag cggacaaact ggcaagcggc gcacgtaaag cgcaattgaa
    14761 tatggtgcgt gaagctgtac tggcgtttaa cggtgatgtg caggttgaaa cgttttcttc
    14821 gttgaagaaa caaggcgtgg acaagctgcg gcagaaactg gatacctggt ttagcgagat
    14881 gcagcctgta gaagaaacgc aggacggcga ataattttct tgccttaatg cttgtgccgg
    14941 atgtggcgta tccggcccgt aaattcaata gattacaatt ctaccgttgg cctgataaac
    15001 atagcgcatc aggcattacg gatcttttct ttcgcccaat aaaaaacgcc ccagtcatta
    15061 ctgactgggg cggctaaaat attcagccaa atccgattac gtgaagtaaa aggtctgaaa
    15121 gatagaacat cttacctctg taccctacgc gactaacttt actctttttt gttcagttca
    15181 gaaagcactt tttgtaattt tttttcagtt gttgcatagg aatttctaat agccatcaca
    15241 aaacgtgaca gcttatgttg cttacttacg aaaaaagggc atgttcaggc gaatcttagt
    15301 gcgcctgatc ccagttttcg ccactcccca cttccaccag caacggcaca tccagacggg
    15361 tacagttttc catcagttga tgaatctgct tcgcgacggc atcaacatca tctttatgaa
    15421 cttcaaatac cagttcatcg tgtacctgca tgatcatacg tacacgcggt tgctcagcct
    15481 gtaaccacgc atcaacggca atcatcgccc gtttgataat gtcggcggcg gttccctgca
    15541 ttggcgcgtt aatggctgca cgttcagccg ctgcacgacg agcaccattg ctggatttga
    15601 tatccggcag atacagacgg cgtccgtcca gcgtttcaac gtagccctgc tctttcgcct
    15661 gagcacgggt gcgttccata tactccagca cgccagggta gcgttcgaag taaaggtcca
    15721 tgtacttctg cgcttcttta cgtggaatgt tcaattgccg cgccagaccg aaagcactca
    15781 tgccataaat cagaccaaag ttgatcgctt tcgcgctacg gcgttgctcg ctggtgacgg
    15841 tttccagtgg caaaccaaac acttctgccg ccgttgcccg gtggatatct tttccttccg
    15901 cgaatgcggt cagcaagcct ttgtcacgcg aaagatgcgc cataatgcgc agttcaatct
    15961 gcgagtagtc cgctgagaca atcacataat cctctggcgc aataaacgcc tggcggatac
    16021 gacgaccttc ttcgttacgc accggaatgt tttgcaggtt aggatcggtt gacgataaac
    16081 gtcccgttgc agttactgcc tggtgataag aggtatgcac acgcccggtt ttcgggttga
    16141 tcatcagcgg cagcttgtcg gtgtaggtcg atttcagctt cgccagacca cgatactcca
    16201 gaatcacttt tggcaacgga tagtccagcg ccagttcttc cagtacctct tccgacgttg
    16261 acggcgcgcc acccggcgtt ttcttcagcg gtttaatacc ctgtttttca aacagaatgg
    16321 tttgcaactg cttggtggaa gaaaggttaa attcttcacc tgcaatttca tgcgctttct
    16381 tttccagctc agccagacgc aaggtgagct cttcagaatg attgtgcagc actttcggat
    16441 cgatcttcac accgttacgt tcaatgcgtg aaagcaccgg caccagcggc atttcgatat
    16501 tctcgaagac gttcaacggc cctttgtgtt tttgcaggtc cggccacatt ttcagatgca
    16561 actgcaaggt gacatcagca tcttcggcgg cgtaacgtcc ggcctcttcg agggcaatct
    16621 ggttaaaggt cagttgattt ttgcctttac ccgcaatctc ttcaaaagtg atggttttgt
    16681 gcttcaacca acgttccgcg aggctgtcca tatcgtgacg cccggcaacg ctattgagaa
    16741 tgtaggactc cagcatggta tcaaacgcaa tcccacgcag ctcaatgccg taattcgcca
    16801 gaataccgcg atcgtatttc aggttttgcc cgaccttcag cgccttttca tcttccagca
    16861 gcggttttag caactcgagt gcacgctcgc gagagatttg atcgggcgca tcaagataat
    16921 catgagcaac cggaatatat gccgctacgc ctggctcgat agcaaaagaa agcccgacca
    16981 ggttagcaga gatgttatca aggctgtcgg tttcggtatc aaatgcaaat accggcgctt
    17041 tttccagctt cgcaatccac gctttcagtg tttcttcatc aaggatggtg acgtagttgt
    17101 cataagaaat caccgttgcc gtcacttctg gcgcttcgtc tgcaacactg gtttcctgcg
    17161 gcttcgcggc tgtttttgcc cctttggcct gtaaccattt gcccgcttcg acatcagcag
    17221 tccagcgttt gaactcatac tttttgaaca gccccaacaa ctcttccgct gccggttgct
    17281 gcacttccag ttgttcacag gtcagctcca gttcaacgtc ggttttaatc gtcgccagct
    17341 ggtatgagag ataagcaact tctttgtttt gctcgagctt cgctgccatt gttttcgcgc
    17401 cacggaagct caacccagca attttttctg gctcggcata cagcgtatcc agcccgccca
    17461 gaccttgcag caatgcctgc gcggtttttt caccgacgcc cggtacgcca gggatgttat
    17521 cggaggagtc acccatcagc gccaggaaat cgatgatcag ttctggcggc acgccgtact
    17581 tattcaccac ctcttccggt ccgaggatgg tattcgtcat ggtattgata agcgtaatat
    17641 ttggcgtcac cagctgcgcc atatctttat cgccagtgct gatcagcacc ggacgcccgg
    17701 ctttttcggc ttcgcgcgcc agagtaccga taacgtcgtc cgcttctacg ccagaaaccg
    17761 ccagcagcgg cagtcccatc gctttaacca tcgcgtgcag aggttcgatt tgtgctcgca
    17821 gatcgtccgg cattggcggg cgatgtgatt tgtaatgttc aaacagttca tcacgaaagg
    17881 tttttccctt ggcgtcaaag accaccgctg catgcgtcgg tttatattgc atgatcagac
    17941 tgcgcagcat gttgaggaca ccatacatcg caccggtcgg ctcgcctgcg ctgttagtca
    18001 gcgggggaaa cgcgtgatat gcgcgataaa gataagatga accatctaca aggataagtg
    18061 gattttgggg gatctgaacc ataatgtccg tgcctgttta tcagattatg ggtaaaggat
    18121 gccacagaca ggataaaaac atgagttttt caccgctttt cgtgaaaatc ttttgtagat
    18181 cttctggatc gctcgcaaat cgtcatgtgg ataactttgt gcataaatta tttacatcgc
    18241 ttttaattta ttatcatgct ttttacagta tgatgtttgt ctttgttttt catgtagtta
    18301 aaaataaagc catccctaat aattgagata atgggatttt ttctaatgtg gatagatatg
    18361 aattattttt ctccttaagg atcatccgtt atttgggtcg ttttctgcta aaatctgcgc
    18421 cttgcgcttc tttagcgcac tgtctatggc taaccttttg aataaattca ttatgacgag
    18481 aatactcgct gcgataaccc ttttgctgag tatcgtattg accattttgg tcactatttt
    18541 ttgttccgtc ccgatcatca ttgccgggat tgtaaaacta ttgctacctg tgccagtcat
    18601 ttggcgaaag gtttcgcgtt tttgtgattt tatgatgtat tgctggtgtg aaggtctggc
    18661 ggtattactg catctgaacc cacacctgca atgggaagtt cacgggctgg aagggttaag
    18721 taagaagaac tggtatctgc ttatttgtaa ccaccgtagt tgggcagata ttgtcgtact
    18781 gtgcgtgctg tttcgtaagc acattccgat gaataaatat ttcctcaagc aacagctggc
    18841 ctgggtgcca tttcttggcc tggcgtgctg ggcgctggat atgccgttta tgaagcgtta
    18901 ttcccgcgct tatttattac gtcatccgga gcgacgtggt aaagatgttg aaaccactcg
    18961 gcgttcttgt gaaaagtttc gcctgcatcc caccaccatt gtcaatttcg ttgaaggctc
    19021 ccgtttcacg caagaaaaac atcagcaaac ccactccact tttcaaaact tgttgccacc
    19081 aaaggcggca ggcattgcga tggcgataaa tgtactgggt aaacaattcg ataaactgct
    19141 taatgttacg ctgtgttatc cggacaataa ccgtcagccg ttcttcgata tgttaagcgg
    19201 taaactgacg cgaattgtcg ttcatgtgga tttacagcct attgccgatg agttacatgg
    19261 cgactacatc aatgacaaaa gctttaagcg ccattttcag caatggctga actcactgtg
    19321 gcaggaaaaa gaccgacttc tgacgtcgtt gatgtcatca cagcggcagg acaagtaaat
    19381 tttgctcacc tctgaatgag cagaggcgag tgagtatttt aatgaacaaa acgtccggca
    19441 cgagacataa attcttcttc gctcatctcc tgtccgttaa aaacaacttt acccggtgta
    19501 taacgcagtt gcagcgaggc ggtattgttg tcaatcgtga ttaactgaaa catctgacct
    19561 aaggtcatca tcccgctgat ttgtttatca gcccgctttt gcgctttttc cgcatccatt
    19621 ccttcagata aattaagctg ttttgcggtt tctgtgacga cattaagcgg taacttcaca
    19681 tcaaaattga gcgatttgat atctttgttt gtggatgatg aagatttggc tgggtcggca
    19741 atactgatat ccagattggc atttagttcg ccgagtgcgt tcttccatct taccggttgt
    19801 ttaatggtcg gctcactttg ttgtaataac ggtaaatact ctttgaacaa tgcagcattc
    19861 acttcttgca gagcaacttc atcgtttgct aactcagggt gtgcagcaag ctgcttctgc
    19921 atcgcaatgt tatactggat gataaattgg cgcactgctg agggataaat agattcagca
    19981 atcaatgacc actcaccact accgagttgc tgattccctt ttgttaactt atcaagttcg
    20041 taggtcagat tgacattgat ataatctttg tctttaacgc gatccagcct ggtttttgca
    20101 acgaaagcat cgatttgggc aacgtcttcc ccctgattga taatattaac ctgattaaat
    20161 ttcgactcgc tttcaccaac cggaaatttc ttttcttcta atcgtgtcag agaatcaatg
    20221 gtaaaacttt ttgccgttac tttgttcatt ggtgataatt gcagaacaat atcttcgact
    20281 ttgccttcaa tatccaggtt agacaatgaa ctaccatcac cattaaaagt aaactggccc
    20341 tcaccaaaag aaaactttcc atactctgca ggggcaacat ctaatgtcgt agagaatgag
    20401 ccaccaaacg cgaatcgcaa tgtcgcttcc acgggcgatt tatttttcgc ggcgataaat
    20461 agcggttgtg tcagttcatt attcactaag ttcacttttg ccgctgccag tgctgggatg
    20521 acattaccat gcattaacat tgtgatgggc aacggaccgt gctcaacatc cacatcaaat
    20581 gcaactttct ggcctggctt gatattgaga tcgggtgcac cgttatcaaa tgaaatttgc
    20641 atttgaaaat gggaattaaa aaagcctttg tcaaagtttt tataacttaa cgtcatatcg
    20701 taggcatgtt cacctttctt tttagcatcg ttaaaatctt taataaattt ttcgacacca
    20761 ggctgaattt gcgtacctgt gtaccatgtg ccaccacccc agattacggc taacgcaaca
    20821 ataacacctg tagctgactt acgtatcata taaatattcc ctgataaaac agcaaaactt
    20881 tgaatatcca cttatgctga aataatgtcc agcgacagga tgtattatca gaatgtattt
    20941 atatttatta aataataaaa aaagcccgtg aatattcacg ggctttatgt aatttacatt
    21001 gaattatttt ttctcggaca gatatttcac tgtatcagca tactgctgaa caaaaacatc
    21061 catattgctg gtatccatac cctgcggatt cagctgatat ttaccgttaa caaacatcgc
    21121 cggaacaccg cgcaattgca cgtcagctgc agctttttcc tgctgagcta ccagagattt
    21181 caccacgaag ctgttccacg ccgcgtcgta ctcttcacct ttaatacctg cgttgataaa
    21241 tacatcgcgg atatcagaag cagaacgaat ggtctgggtt ttctgtacgc cttcaaacag
    21301 cggaacagtc actttgtctt ccacgcccag cgccatcgcc acagcccatg cctgagtcag
    21361 atctttgccc aggtcaccac ccatgaagtt gacgtggtat ttagtcatct tcacgccttc
    21421 cggcagtttt ttcttcacat tatcagaaat atgcagaact tcttcaaact gatagcagtg
    21481 cggacagaag aaagagaaaa actccagcac ttgcggcgcg ccagctaccg gtttttccag
    21541 ggtagtgtac tgtttaccat cttcatactg cgccgccgat gcgctaaacg ctaaaactaa
    21601 accagccagc gccagccaaa tctttttcat gatctactct ctccgattaa tacataggtg
    21661 ttaattgtaa agggggttct tgtagaactt ttgcctgttc tataaaagtc gccgtttgtc
    21721 gcagccagta atcttccccg gttaaccacg ggaaattttt cgggaacgcg ggatcagccc
    21781 aacgccgcat tagccaggca agataataaa ccaaacgcat ggcgcgtaaa ggttcaatca
    21841 gtccgatttc agcggtgtcg aactcgctaa attcttcata agcttcaata atagtttcca
    21901 gttgcatccg ctgctcggct ttatcgccat tgagcaacat ccacaaatcc tgaatagctg
    21961 gaccattacg tgcatcatcc agatcaacaa acattggacc atcgcgccag agaatattcc
    22021 cggcgtggca gtctccatgt aagcgcagaa cggtggaatc ttcccgccag tgcgctgtaa
    22081 cggcggcgat cagctcatct gtcgctttca ggaatgccgc tttcaacccg gaaggtatca
    22141 gtgtagcgtc ctcaaacagc ttgcgtggct caatgagata ttcattcaga ccgatggtcg
    22201 ggcgatggat aaaaagctgt ttgcgccccg tctggtgcat acgccctaaa taacgcccaa
    22261 ccgcttccat ctgatcgata ttatcagctt cgaactggcg accaccgacg cttggaaaaa
    22321 cagcgaaata aaatccctga tgattcaata aagtctgacc gttaaaggcc acaggtgctg
    22381 cgaccggaac ttcatcattt accaactgca acgcaaattg atgttcttcg aggatttgat
    22441 cggctgtcca acgttcaggg cgataaaatt tgacgacaat acgtcgaaga tcttcgtcct
    22501 gaaattgata gacacggttt tcatagctgt taagcggggt aagaccggaa tccacccgga
    22561 tcccttgctc aaatagagca tccatgatgg tatccgggtg tagtgtctgg aaagtaaaag
    22621 cgctgttgtt catcccatca tccggaaaac attacgaata atgaaggata tcatttcacg
    22681 atgattttgg tggcgccgct tacaagcttt tactctttaa ttacgccgcg agcgcgtaac
    22741 agcgccgttt tgaagtcttc ttcataatct ttctgcaaac ccgggatcac cgcatctttc
    22801 gcggaatcac gcattttcag gtgatagatc agaatgtcat ccgtcaaatc cgccagttcg
    22861 ccgtcaaaac ctgactcttt cgccagtttc tgcaaaaatt gcaacaggtt aaggtctggc
    22921 tctttttgcc aggctggctg gaggaattca ataacttcat tcagacgttt acatttcata
    22981 gtgatgctcc ttactcttga gacagacacg ttagcagggt caatcccaca ataaaagagg
    23041 cgatatcggt gaatctgatg acgacgataa caggcgttgt gctggcaggc ggtaaagcca
    23101 gacgaatggg cggcgtagat aaaggattgc ttgaattaaa cggcaaacca ttatggcaac
    23161 atgtcgctga cgcgcttatg acgcagctct ctcacgtcgt ggttaatgcc aatcgtcatc
    23221 aggaaatcta tcaggcaagc ggtctgaaag tgattgaaga ttcactggcg gattacccag
    23281 gccctctggc aggaatgctt tcagtaatgc agcaggaagc tggtgagtgg tttttgtttt
    23341 gcccgtgcga tacgccttac attccccctg atttagcagc ccggcttaat catcagcgca
    23401 aagatgcgcc tgtcgtgtgg gtccatgacg gtgaacgcga tcacccgact attgctctgg
    23461 taaaccgcgc tattgagcct ttattactgg aatatctgca agcaggagaa cgccgggtaa
    23521 tggcatttat gcgtctggct ggcggtcatg cggttgattt cagcgatcat aaagatgcat
    23581 ttgttaacgt gaatacgcca gaggagcttg cccgatggca ggaaaaacga tgataccgtt
    23641 actcgccttt gccgcgtgga gtggtaccgg aaaaactacg cttttgaaaa aactgatccc
    23701 ggcattatgc gccagaggga tccgtccagg gctgattaag catacgcacc atgatatgga
    23761 tgttgataag ccaggcaaag atagctatga gctgcgcaag gctggcgcgg cacaaaccat
    23821 cgttgccagc cagcaacgat gggccttgat gacggaaaca ccagacgaag aagagctgga
    23881 tctacagttt ctcgcaagtc gaatggatac ctcaaagctg gatttgattc tggtcgaagg
    23941 gtttaagcat gaagagatcg caaagattgt gctgtttcgc gatggagccg gacatcgacc
    24001 tgaagaatta gtgatagaca ggcatgttat tgctgtagcc agtgatgtgc cgcttaatct
    24061 tgatgtcgca ttactggata ttaatgatgt tgaggggctg gctgattttg tggtggagtg
    24121 gatgcaaaag cagaacggat aagcaagagt aggctggatc agacgcagcc ag
//
LOCUS       DAJTMK010000048        24036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTMK010000048 DAJTMK010000000
VERSION     DAJTMK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.049"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..304)
                     /locus_tag="OO135_001642"
     CDS             complement(<1..304)
                     /locus_tag="OO135_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR5397537.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANT"
     gene            complement(588..878)
                     /gene="ubiK"
                     /locus_tag="OO135_001643"
     CDS             complement(588..878)
                     /gene="ubiK"
                     /locus_tag="OO135_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5397538.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      1000..1148
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1252..1905
                     /gene="ribB"
                     /locus_tag="OO135_001644"
     CDS             1252..1905
                     /gene="ribB"
                     /locus_tag="OO135_001644"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5397539.1"
                     /translation="MNQTLLSSFGTPFERVENALAALCEGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            2166..2336
                     /locus_tag="OO135_001645"
     CDS             2166..2336
                     /locus_tag="OO135_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397540.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(2394..3155)
                     /gene="zupT"
                     /locus_tag="OO135_001646"
     CDS             complement(2394..3155)
                     /gene="zupT"
                     /locus_tag="OO135_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5397541.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSNGVLCGMSVMGFSLVLLQTAGIG"
     gene            3298..4086
                     /gene="ygiD"
                     /locus_tag="OO135_001647"
     CDS             3298..4086
                     /gene="ygiD"
                     /locus_tag="OO135_001647"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5397542.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(4124..5284)
                     /locus_tag="OO135_001648"
     CDS             complement(4124..5284)
                     /locus_tag="OO135_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5397543.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(5290..5961)
                     /locus_tag="OO135_001649"
     CDS             complement(5290..5961)
                     /locus_tag="OO135_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5397544.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(6109..7590)
                     /gene="tolC"
                     /locus_tag="OO135_001650"
     CDS             complement(6109..7590)
                     /gene="tolC"
                     /locus_tag="OO135_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5397545.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7795..8424
                     /gene="nudF"
                     /locus_tag="OO135_001651"
     CDS             7795..8424
                     /gene="nudF"
                     /locus_tag="OO135_001651"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5397546.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            8425..8847
                     /locus_tag="OO135_001652"
     CDS             8425..8847
                     /locus_tag="OO135_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5397547.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8872..9699
                     /gene="cpdA"
                     /locus_tag="OO135_001653"
     CDS             8872..9699
                     /gene="cpdA"
                     /locus_tag="OO135_001653"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5397548.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9699..10280
                     /gene="yqiA"
                     /locus_tag="OO135_001654"
     CDS             9699..10280
                     /gene="yqiA"
                     /locus_tag="OO135_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5397549.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            10309..12201
                     /gene="parE"
                     /locus_tag="OO135_001655"
     CDS             10309..12201
                     /gene="parE"
                     /locus_tag="OO135_001655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5397550.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLMQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(12249..12563)
                     /locus_tag="OO135_001656"
     CDS             complement(12249..12563)
                     /locus_tag="OO135_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5397551.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12594..13175)
                     /gene="mdaB"
                     /locus_tag="OO135_001657"
     CDS             complement(12594..13175)
                     /gene="mdaB"
                     /locus_tag="OO135_001657"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5397552.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(13285..14634)
                     /gene="qseC"
                     /locus_tag="OO135_001658"
     CDS             complement(13285..14634)
                     /gene="qseC"
                     /locus_tag="OO135_001658"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5397553.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNSPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(14631..15290)
                     /gene="qseB"
                     /locus_tag="OO135_001659"
     CDS             complement(14631..15290)
                     /gene="qseB"
                     /locus_tag="OO135_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5397554.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15442..15834
                     /gene="ygiW"
                     /locus_tag="OO135_001660"
     CDS             15442..15834
                     /gene="ygiW"
                     /locus_tag="OO135_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5397555.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQRPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15887..16369
                     /locus_tag="OO135_001661"
     CDS             15887..16369
                     /locus_tag="OO135_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5397556.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            16915..19173
                     /gene="parC"
                     /locus_tag="OO135_001662"
     CDS             16915..19173
                     /gene="parC"
                     /locus_tag="OO135_001662"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5397557.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19406..20143
                     /gene="plsC"
                     /locus_tag="OO135_001663"
                     /gene_synonym="parF"
     CDS             19406..20143
                     /gene="plsC"
                     /locus_tag="OO135_001663"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5397558.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20218..21630
                     /gene="ftsP"
                     /locus_tag="OO135_001664"
     CDS             20218..21630
                     /gene="ftsP"
                     /locus_tag="OO135_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5397559.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLIENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21741..23954
                     /locus_tag="OO135_001665"
                     /pseudo
     CDS             21741..23954
                     /locus_tag="OO135_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
BASE COUNT         5675 a         6013 c         6420 g         5928 t
ORIGIN      
        1 tagtattcgc tgtggtgaaa gtaaccttcg cttttgaata agtggttcca ccttggctca
       61 acatgcagtc ttgcaggtga atagtgaaag gctcagccaa agatttctgg tcgcttttca
      121 gaacggtatc tgctacttca cccagattta cagttaagtc ttcgtcacct ggtttaattg
      181 aacatggagc agagataact tcacctttaa atttaacaat gccgtgacct tcgtcagcaa
      241 atacgttatt ggaaagaatc attgctacgc cagcaacaag aagtgacttt ttaaaagcag
      301 acataatatt cctttatata aatataagta gaaaatgaat gattagtaac tgttatttaa
      361 ggattgctaa tcattcaaat attatatttt acttttcggg ttttatgaaa ttagtttatc
      421 taaatatgga ttaagtaaaa cttaaaaaaa tacataacaa ttatattttc aattgcactc
      481 attcattttt gtgatgatat tctgaatgga attaagcgat aaggtaatag aaagcactgt
      541 aaattagaag attaattcat aatttacagt gcattaaaga caaatgttta cagcgttggg
      601 gggagagtct ctggatctgg ctgcttttta atctcagtac tgcgattttc cagttcgctc
      661 attctttgtt caagcagcgc cagtttctca cgagtccgta acaggacttg cgtttggacg
      721 tcgaactctt cacggcttac aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg
      781 atttttttct ccacatcttc cccgaactcc ctgattcctt taggcattga ttcgtgaacc
      841 tggcgagcga tttgctcaat ttttttcggg tcaatcattg tagtttccct gtactgatag
      901 gtgttgagta ccattgtagt gcgataaggg taagtcataa accagaatta tgtgaagcta
      961 tgcgttgctg ccgctaatca ttagcgttat agtgaatccg cttattctca gggcggggcg
     1021 aaattcccca ccggcggtaa atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa
     1081 ctcaaaggac agcagatccg gtgtaattcc ggggccgacg gttagagtcc ggatgggaga
     1141 gagtaacgat tctgtcgggc atggacccgc tcacgttatt ttggctatat gccgccactc
     1201 ctaagactgc cctgattctg gtaaacataa ttttagtgag gtttttttac catgaatcag
     1261 acgctacttt cctcttttgg tacgcctttc gaacgtgttg aaaatgcact ggctgcgctg
     1321 tgtgaaggac gcggtgtaat ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg
     1381 atcttcccgg cagaaaccat gaccgttgag cagatggcgc tgaccattcg ccacggtagc
     1441 ggtattgttt gcctgtgcat cactgaagat cgccgtaaac aacttgatct gccaatgatg
     1501 gtagaaaata acaccagcgc ctatggcacc ggttttaccg tgaccattga agcggctgaa
     1561 ggtgtgacta ccggtgtttc tgccgctgat cgtattacga ccgttcgcgc agcgattgcc
     1621 gatggcgcaa aaccttctga tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag
     1681 gcaggtggtg tactgacgcg tggcggtcat actgaagcga ctattgattt gatgacgctg
     1741 gcaggcttta aaccggctgg tgtgctgtgt gagctgacta atgacgatgg cacgatggcg
     1801 cgtgcaccag agtgcattga gtttgccaat aaacacaata tggcgctcgt gactattgaa
     1861 gacctggtgg cataccgtca ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat
     1921 tactgcctta atcaagaaac cgaagttgta gctggcttcg gtttttattt tgccctgcta
     1981 tgcaagatgt taattccgct tctctttcta tgagaaaatt tcattaatat caggcattct
     2041 ttttcattat gatcaaaatc atataattgt tccgttatta tgcgaccata tgaatatatc
     2101 cgaaaaagga tatgtgaaat ttatgcgcta aatgtttagt attgttaaat attaagggta
     2161 aaaggatgtt tatcgcctgg tactggattg tattgattgc tctggttgtg gtgggttatt
     2221 tcctgcattt gaaacgttat tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag
     2281 cgcggaacaa atacttaaac agtacgagag aagagacagc cgaaaaggta gaataaggct
     2341 ttcgtgaagc tggcagaagt tgtccatgtg ctggacaact tctgatgttg tttttaacca
     2401 attcccgccg tttgtagcag cactaaactg aatcccatca ctgacattcc acatagtacg
     2461 ccattgctgg ggttattatt agggtcaatc tctttggcga gcggcattaa ttcatcaacc
     2521 gagagcgcca ccataattcc tgcaaccgcc gccatgacca ccggggaaat catgctaccg
     2581 aggattaacc acgccagcac accaccaaga atttctgcta gtccagaaat ccccgcccac
     2641 agaatcgcgg tgcgtttaga ccccgttgcc gcataaaccg ggcctgccac tgccagacct
     2701 tcagggatat tgtgcaacgc gacggccagt gcgatgccaa atcccagctc caggttgctg
     2761 ctcgccgtga caaaggtggc aatcccttcc gggaagttat gcagactgat gccgagagtg
     2821 agcagaatgg ctgtgcgctt gatcgatttt ggcaacggct gcaccgattt ttgcattaaa
     2881 tcctgcggat gcgcatgcgg caacatgcgg tccaggccaa agtagccaag cagaccgaag
     2941 ataaacattc cataacccaa cacaggcgac attccttcag ctgctagtgc ggcaggaagc
     3001 atttccatta atgagatgag caacatgatc cccgccgcaa aacctagcga aaacgccagt
     3061 aagcggttcg agggtttttg tccgagaacg ccgagaaacg cgccaataaa cgtggctgcc
     3121 cccgccagta tggtcagaat gagaggtact gacatcgaca actccttata aatcttcacc
     3181 ttcaggcaga tcgtcaaaat aactgatgat catcatcatt gttatccggg ttcttactcc
     3241 gccgaacaag cgtattgtga ggatatcaaa atgacaccct tagttaagga tatcatcatg
     3301 tcttcaacac gtatgccagc attgttttta ggtcacggta gtccgatgaa cgtgctggaa
     3361 gataatttgt atacccgcag ctggcagaag ttggggatga cattgccacg cccgcaagcg
     3421 attgtggtgg tttcggctca ctggtttacc cgtggaacag gagtgaccgc gatggagacg
     3481 ccgcccacga ttcatgactt tggtggcttc ccgcaggcgc tgtacgatac gcattatcct
     3541 gctccgggtt cgcctgtgct ggcacagcat ctggttgagc tgttagcgcc ggtcccggtg
     3601 acgctggata aagaagcctg gggctttgac cacggttcct ggggcgtgct gattaagatg
     3661 tatcctgacg ccgatatccc gatggtgcag ttgagtatcg acagtagcaa acctgccgcc
     3721 tggcatttcg aaatggggcg caaactggca gcgctgcgag atgaaggaat aatgttggtc
     3781 gccagtggca acgtggtgca taacctgcgt acagtgaagt ggcacggtga tagttcacca
     3841 tatccttggg cgatgtcgtt taatgagtat gtgaaagcga atctgacgtg gcaaggggcg
     3901 gtagagcagc atcctctggt gaattacctc gaccatgaag gtggcgcgtt atcgaaccca
     3961 acgccggagc actatctgcc gttgttgtat gtcttaggtg cgtgggatgg gcaggagcca
     4021 attaccattc cggtcgatgg tatagaaatg ggcagcctga gtatgctgtc ggtgcagata
     4081 ggttaacaat taccggatgg cgaatgccat ccggcatcat tgattactca acaaaaatat
     4141 gtggataaaa ccgagacata tcctgggtga tcaatgcacg gtcttcacga atgccaattc
     4201 cggcgggttg atcgttcacc agccagctac caatcagcat atagctgtcg ccgaatttcg
     4261 gtaacggatg gaattgctga acaatcatcc cttcttcgcc atacggacct tccgctgctt
     4321 caatggtttt gccgttctca atgatcgaca cgtttgcgcc ttcacgggag aagatcggtt
     4381 taaccacata tttttccatt tgcggatgat catcttccgc aaaataagcg ggcagcaggt
     4441 tcgggtgatt cgggaacatc tcccacagta gcggtagaag cgctttgttg gagataatgc
     4501 tcttccacgc cggttccagc cagcgtacgc ctgcatcctc cagcttggtg gagaacatct
     4561 cacgcaacat aaattcccat ggatacagct tgaacaggtt ggaaattacc tgatcctgta
     4621 aatccgtgaa ctgacctttt tcacctaacc cgatatcgtc aatgtagagg aactcggtag
     4681 cgatttcagc ttccgtcgcg cagtcctgca aatactgaat ggttccgcga tcttccaccg
     4741 tgtcgcgaca gcaggtgaga tgcagcaact ggaagccata ctgttcacgc aactcgacaa
     4801 agcgatcgat tagcttttct tgcagactgt taaactggtc gctgccctcc ggcaagttac
     4861 cggcgttaag ctgatcttcc agccagatcc actgaaagaa cgccgcctcg tatagtgacg
     4921 ttggtgtatc ggcgttattt tccagcagtt taggttcgcc agtgccatcc catgccagat
     4981 caagacgcga ataaagcgat ggctggtgag tcagccatga ctgacgcaca aaactccagg
     5041 tgtgttttgg aatgcggaat ttggtcatca gctcatcgct ggcgatcact ttttccacca
     5101 ctttcaggca catctggtgc agttcggcgg tgacttcttc cagcttttca acctgggcga
     5161 gggtcaactt gtagtaagca tcttcacacc agtacggctc gccgtacatg gtgtgaaaat
     5221 tgaaaccgta ttcgtgggct ttttcacgcc agtccgggcg ctcggtaata ctgactcttt
     5281 ccatcggtat cagccaccca ttgaacgaga agaggtgccg gttgcgctac gctgcatagt
     5341 gctttgtttg gcaacagatt caccaaaacc gccacgggta acggtagtag tggtcgccgg
     5401 ttttggtgcc atggctgtct tcggtacggt catggtgcgg cctggctggg ctgcgccata
     5461 gtttttaccc gtcgcgtcgg tatatttacc gtaagccggg ctggctgggt ttttcgagga
     5521 gaacagcggc tgctgtgcaa atcccgcgcc gccgcccatc agacgcccca tcatgtaacc
     5581 ggccatcagc ggcatccaga aactcccgct ggattgctgg gcctgcgcct ggttttctgg
     5641 tgccatgcca gcctgggctg gtgcctgctg gcactgacct tcaccaaatt cagcaacaca
     5701 gtcttcacgg gtggcgtatt tcggcgcagt acgttcggct tctttcagcg cattgttgta
     5761 cgcggtggta cattcggcgc ttttgcctgg gtttgcagct gaacagtcgt cagcattttg
     5821 atagagagac actgtttcat cactcttttc acagctagcc agcataaaaa cagtggcaac
     5881 cgcgagagcg actggtgtca gatggcgtgc gctccagttt ttgcggaacg atgcgtggcg
     5941 tatggatttt gtccgtttca tttttgtctt ccgggaccag tggtaaatac ccatcagaat
     6001 agaggatggc tggtcgaaat tgaagcgaga agggggaaga atgcggcaga taacccgtat
     6061 ctttacgttg tcttacgttc agacggggcc gaagccccgt cgtcgtcatc agttacggaa
     6121 agggttatga ccgttactgg tggtagtgcg tgcggatgtt tgctgaacga cgggtgccgg
     6181 gctatcaggc gcataaccat cagcaatagc attctgttcc ggcgtttgcg gggcaacgtt
     6241 ttccggatta gtggaaaccg gtttgctcag cgcattgttc agtgccagca gatcctgctc
     6301 gttcaacgta cccagggctg acttaatatt cagctgatta atcaggtagt tataacgcgc
     6361 atttgccagc tcttgcttag cgttgtacag cgtggtggtt gcatccaaca catcaacaat
     6421 ggtacgcgta ccgaccgagt agcccgcttc catcgcgtct aatgagcttt gagcggaaac
     6481 tacggcttgt ttgtaggcgt taatgctact gatagatgca ttaatgttgt tgaaggagga
     6541 acgtacggtt tgcacgatgc tacgatgcgc gctttccagt tgctcgctgg caccaacaaa
     6601 gttgtactgg gcctgtttca cctgcgagtt aaccattccg ccctgataaa tcggcagcga
     6661 gaagctcagg cccactttgt tctggcccat attgctgtcg tcatactggg taccagcggc
     6721 accacgagtt ttcgaaccgc tataagaggt gtcagaaatc ccggtagaag ccgttaaatc
     6781 cagcgtcggt aagtgaccat cctgcgcctg gcgaatttgc tcgcgcgcca ggtcctggct
     6841 caagcgtgcc tgtaacagcg acaggttgcg tttttcggct tctttcagca gcgcgttaac
     6901 cggctgtggt ttgtcggttt taaagttttc gacattcagc gccgccagtt ccggatagta
     6961 gttaccggtg atctggcgca gctgctctac cgcgttatca aggttattac gtgcggtcac
     7021 ttcgttcgcc agcacggtat cgtactgcgc gcgggcgttc tgcacgtcgg tgatcgctac
     7081 caggcccacg ttaaaacgtt gggtggtttg atctaattga cggtagatcg cttctttttg
     7141 tgcctgtgta taggaaagaa cgtcaatagc attcaacacg ttgaaataag cggtcgcggt
     7201 gttgaggatc aaggtttgct gatcggtctg atatgtgacg tcctgaatcc ctgctgcttt
     7261 ttcctgcagc gttaacgcac gccatttcga catatcaaaa atggattgag ttaactgcag
     7321 ggacgcactg gtcgcgttcg agttgatgcc gttcgcgtcg cggtagccgt tgctataggt
     7381 gtaatctgca cctaaaccta gctgtggcag taatggactg cgcgcttcat taattttttc
     7441 aaaggcagca tcacgatcgg cggcagactt acgcaattcc gggttactaa ggcgtgcttg
     7501 ctgataaact tgcatcaggt tctcggcctg gctcaacgaa ctgaacccag aaaggctcag
     7561 gccgataaga atggggagca atttcttcat ttgcattcct tgtggtgaag cagtatttag
     7621 cgcgatcaaa ctgtaaaatt attgcggatt ctagcagaag ccgctaccgc aaaaggttgg
     7681 cgttacgtgc catagggcgt taatgtgcct caatttaaca catgactgct caaacggcag
     7741 tcaaacgtcg ctgaaattca catttaattc actattagtg ccaggacatt aacaatgctt
     7801 aagccagaca acctgcccgt tacatttggc aaaaacgatg tagaaattat tgcacgagaa
     7861 acactttatc gcggcttttt ttcattagat ctttatagat ttcgtcatcg tctattcaac
     7921 gggcaaatga gtcatgaggt gcggcgggaa atttttgagc gcggtcacgc cgcagtcttg
     7981 ctaccctttg acccagtgcg tgatgaagtt gtgctgattg agcagattcg aattgccgcg
     8041 tacgacacta gcgaaacccc ctggctactg gagatggttg ccgggatgat tgaagagggt
     8101 gaaagtgtgg aagatgttgc ccgtcgcgaa gcgattgaag aggcggggct gatagtcaaa
     8161 cggaccaaac cggtgttaag tttcctggca agcccggggg gcaccagtga gcgttcgtca
     8221 attatggtgg gcgaagtgga cgccacgacc gcaagcggta ttcatggtct ggctgatgaa
     8281 aacgaagata ttcgcgttca tgtggtaagc cgggaacagg cataccagtg ggtagaagag
     8341 gggaaaatcg acaacgcagc gtcggtcatc gctttgcaat ggctgcagct gcaccatcaa
     8401 gcgttaaaaa atgagtgggc ataaatgaag cgttacacac ctgactttcc tgaaatgatg
     8461 cgcctgtgcg agatgaactt ttcacaattg cgccgtttgt taccgcgcaa tgacgcaccc
     8521 ggcgaaactg taagctatca ggtggcaaac gcacaatatc ggctgacgat tgtggaatcg
     8581 acccgataca ctaccctggt gacaatagaa cagactgcgc ccgcgatcag ttactggagc
     8641 cttccgtcaa tgacggtgcg tctgtatcat gacgcgatgg tggctgaagt gtgttcaagc
     8701 cagcagattt ttcgcttcaa agcgcggtat gattatccta ataaaaagtt gcatcaacgc
     8761 gacgaaaagc atcaaattaa ccagttttta gcggactggt tgcgatactg tttagcacat
     8821 ggagcgatgg cgattccggt ttactagcgt cgtgaaacct aaggacacca tttggaaagc
     8881 ctgttaaccc ttcctctggc tggtgaggcc agagtcagga ttttacaaat taccgatact
     8941 cacctgtttg cacaaaagca cgaagccctg ttaggggtaa acacctggga aagctaccag
     9001 gctgtgctgg aggcgattcg tccacatcag cacgaattcg acctgattgt cgcgacaggt
     9061 gatttagcgc aggatcaatc ctctgcggcc tatcagcatt tcgctgaagg catcgcaagt
     9121 tttcgtgcgc cctgcgtctg gctgccgggc aaccacgatt tccagcccgc gatgtacagc
     9181 gcgttacagg atgcgggtat ctccccggcg aagcgcgtgt ttattggtga gcaatggcaa
     9241 atcctgttgc tggatagcca ggtgtttggc gtgccgcacg gtgagctgag cgagtttcag
     9301 cttgagtggc tggaacgtaa actggccgat gcgccagaac gccatacgtt gctgctgctg
     9361 catcatcatc cgctacctgc gggttgtagt tggctcgatc aacacagtct gcgtaacgcg
     9421 ggcgaactgg ataccgtgct ggcgaagttt ccgcacgtca aatacttgct gtgcggtcat
     9481 attcatcagg agctggatct cgactggaac ggtcgccgcc tgctggcaac gccgtcgacc
     9541 tgtgtgcagt ttaagccgca ctgttccaac tttacgctgg ataccatcgc gcccggctgg
     9601 cgtactctcg agttacatgc tgatggcacg ctgaccaccg aggtgcatcg cctggcggac
     9661 acacgtttcc aacctgatac cgcttcagaa ggctactgat gtctacgctt ctttatttac
     9721 acggtttcaa cagctcgccg cgctctgcaa aagcgagctt gttaaaaaac tggctggcgg
     9781 aacatcaccc tgacgttgag atgatcattc cgcagttgcc gccgtatcct tccgacgcgg
     9841 cagagctgct ggaatccatt gtcctggaac atggcggcga ttcgctgggt attgtcggtt
     9901 cgtcactggg gggatattac gccacctggt tgtcacaatg ttttatgctg cccgcagtgg
     9961 tggtaaaccc ggcggtgcgc ccgtttgaac tgctgacgga ctatctcggt cagaacgaga
    10021 acccctacac cgggcagcaa tatgtgctag agtcacgcca tatttacgat cttaaagtca
    10081 tgcagattga cccgctggaa gcgccggatt tgatctggct gctgcaacag acgggagatg
    10141 aagtgctgga ttaccgccag gcggtggcgt actacgcttc ctgccgccag actgtcatag
    10201 aaggcggcaa ccacgcattc acgggcttcg aagattattt caacccgatc gtcgattttc
    10261 ttggtctgca ccatctctga cgatcaactc gaattactaa cttaaaccat gacgcaaact
    10321 tataacgctg atgccattga ggtactcacc gggcttgagc cggttcgccg ccgtccgggg
    10381 atgtataccg ataccactcg ccctaaccat ttggggcaag aagtcattga taacagtgtg
    10441 gatgaagcac tggcgggtca cgcaaaacgc gtggacgtta ttttacatgc tgaccagtcg
    10501 ttagaagtta ttgacgatgg gcgcgggatg ccggtggata ttcacccgga agagggtgta
    10561 ccggcggttg aactgattct ttgccgtctg catgcaggcg gtaaattctc taacaaaaat
    10621 taccagttct ctggcggcct gcatggcgtg gggatttcgg tggttaacgc cctgtcgaag
    10681 cgcgtagaag ttaacgtgcg ccgcgatggt cagatttata acatcgcctt tgaaaatggc
    10741 gaaaaggtgc aggatttaca ggttgtcggc acttgcggta aacgcaatac cggcaccagc
    10801 gtccatttct ggccggatga aaccttcttc gatagcccgc gtttttctgt ttcacgcctg
    10861 acgcatgtgc tgaaagccaa agcagtactg tgccccggcg ttgagattac ctttaaagat
    10921 gagatcaaca acaccgaaca gcgctggtgc tatcaggacg gtctgaatga ttacctggcg
    10981 gaagcggtaa acggtttacc gacgctgcca gaaaaaccgt ttatcggtaa tttcgctggc
    11041 gatactgaag cggtggactg ggcgctactg tggctgccgg aaggcggtga actgctgacc
    11101 gaaagctacg tcaacctgat cccaacgatg cagggcggta cccatgttaa tggtctgcgt
    11161 cagggcctgc tggacgcgat gcgtgagttc tgtgaatacc gcaacattct gccgcgcggt
    11221 gtaaagctgt cggcggaaga tatctgggat cgctgcgcct atgtgctgtc agtaaaaatg
    11281 caggatccgc agtttgccgg gcagaccaaa gagcgtctct cttcgcgtca gtgcgcggca
    11341 ttcgtttctg gcgtggtgaa agatgccttt atcctgtggc tgaaccagaa cgttcaggcg
    11401 gcggagttgc tggcagagat ggcgatttcc agcgcccagc gtcgtatgcg tgcggctaaa
    11461 aaagtggtgc gtaaaaagct gaccagcggt ccggcgctgc ctggcaaact ggctgactgt
    11521 accgcgcagg accttaaccg caccgaactg tttcttgtgg aaggtgactc cgcaggcgga
    11581 tctgccaagc aggcgcgcga tcgcgaatat caggcgatca tgccactgaa aggtaagatc
    11641 cttaacacct gggaagtctc ttccgacgaa gtgctggctt cgcaggaagt gcacgatatt
    11701 tcggtagcga tcggtatcga tcctgacagc gacgatttga gccagcttcg ttacggcaag
    11761 atctgtatcc tggcggatgc tgactccgat ggtctgcaca ttgccacgct gctctgcgct
    11821 ttgtttgtaa aacacttccg cgcgttggtg aaacacggtc acgtttacgt cgcactgcca
    11881 ccgctctacc gtattgatct cgggaaagag gtttattacg cgctgacgga agaagagaaa
    11941 gagggcgtac ttgagcaatt aaaacgcaag aaaggcaagc cgaacgtcca gcgttttaaa
    12001 ggtctggggg aaatgaaccc gatgcaattg cgcgaaacca cgcttgatcc gaacactcgc
    12061 cgtctgatgc agttgactat cgatgatgaa gacgatcagc gtactgacgc gatgatggac
    12121 atgctactgg cgaagaaacg ctcggaagat cgccgcaact ggttgcaaga gaaaggcgac
    12181 atggcggaga ttgaggttta aaggaaagaa cattgcccgg tcaggatggc cgggcaaaca
    12241 aggcaggatt aaatccctgg ctgcagaata cgaatattca tctccagcac gtcacctttt
    12301 acggcttcgc tgtacgcctt catgtgcggg gtttgcagat gcgcttcaag gtgcgcgatg
    12361 ctttcccact gctcaatcat cacgatagaa tccggtgcca tagactggaa actcacgcca
    12421 gcagcgcaat ccaccattgg cgcatagccg tggcaacctt cttctttcag tacggttgga
    12481 acgattttag caaactgatc caataccgcc tgacggtgat gttgaccagg acgagtacgg
    12541 atttctgcga ttacggtaag catggttaac tccttctaaa gcctgagctc tagttaacca
    12601 aaaatttcca caagatgctt gcgatattct tcagtatagc ggggaacatc aggcattttt
    12661 atcacgtcat tagcgataaa tgtcggcagc ggttccatgc caaggaattg gtttgcttta
    12721 tggaacggca gatacacacc gtcgacgcca acgccgtgga agaattgatc tttttcggtg
    12781 aaggcttcca ttggtgcgtt ccaggtcaga gaaagcatat attttttgcc ctgtaccagg
    12841 ccgccggaac cgtatttttt tgacggatct ttgcgggtac ggccatcgct ggcatacagc
    12901 gtcccgtgac cttcggtgaa tacatcatca atgtattttt tcactgtcca cggcgcgccc
    12961 atccaccagc ctggcatctg ccagatcacc acatcagccc agagaaagtt ttgtacttcc
    13021 gctttgacat cgtagtcgct gtcggcgcga acgatgcgaa catcatgccc gaggtcgcgc
    13081 agtgtgccat ccgcgacttc ggtcagggtg tcattcagtt gaccattgga gtgggcgaat
    13141 tttttcgcgc cgttgataat cagaatgttg ctcatttttt aacctcaagg tgagacgttt
    13201 gccgactata gtacgtcaca gagcggtacg gtgaaattag caaaatgtgc aaagtctttt
    13261 gcgaatttag caaaagtctt acccttacca gcttactttc gcctcaaatc caccttgttc
    13321 cgcattccca aactcaacat tcatgccatg cagcttggcg attcgctgga caatcgatag
    13381 cccaagtcca ctgccggtag cggtttgtcc gggggggcga tagaagcgtt cgccaattcg
    13441 cgccagtgcc tctggtgtca caccggggct gttatccctc acgatgaaat tatcggcatt
    13501 cagcgtgacg tccaccacgc tgccctgtgg actgtaacgc acggcgttat ccagcaaatt
    13561 tcgcaccaac aaacttagca atagcggttg tccggtgcgt ttgatgccct ggacattgag
    13621 tgtcagtcgc acgtcaattt tcgcctgctg cgccgtgtgg taaatatcca tcaccgacga
    13681 ttgcaggaga tcttcaagcg ggatctccgc gacgtcctga aggttatcca gtgagtccag
    13741 ccgcgatagc gtgagcagtt gatcaaccag acgagtagcg cgatcgatcc cggaatgtaa
    13801 ttggagtagt gcttttttcc gcgcctgcgg atcatcgtca gagagctgcg caacttcggt
    13861 ttgcactttc agcgctgtta acgggctacg tagttcgtga gctgcatcgg aggtaaagcg
    13921 tcgttctcga accatcatcg catgtgtgcg ggcgaacagt tgatttagcg actcaaccag
    13981 tggtcgcact tcgctgggta cgccagtcgc gtttagtggt ttttccgagt cagggtcacg
    14041 catacgtagt gccagcgcca gtttgttcag cggcgcgagt tcacgaccca gtagtaccat
    14101 catgatgatt aacataatcg gcagtgcgac cagccacggg atcaattgcc cggcaacaat
    14161 cgccagcgcc atgtcttcac ggtattccca ttcctggcca acaacgatgc gatatttgcc
    14221 atcaggtgag gtcatccaga caaaacgcca aggatcgtct tcaccgacca gttgcccgtc
    14281 agcaaaacct tcccgttgat agctataggg aatatcttct ccgttatcgc catcattaag
    14341 gaccattctg ccgtcgtggg taaagatggc aaaggtcagc gcatcgtcat caacatgacc
    14401 gtgttttaat ttatttggcg tctgtgccat gcgatccgcc gcgttgattt cgttgagatc
    14461 gagcgtactt aaccgcttgg caaacagcat cagttgggtg tcgaacaatt catcgacgtt
    14521 atccgttgtt tgtttccagg cgacaaagct ggaaagcagc caggtcaccg aggccagaat
    14581 taaaaagatt agcgtcagcc tgacgcgcag actaagacgt tgggtaaatt tcatttctca
    14641 cctaatgtgt agccaatacc atgcacggta cgaataaaat cactaccgag tttgcgtcgc
    14701 agatgatgca catgcacttc aacggcatta ctggtgacct cttcgtccca ggtatacagt
    14761 ttctcttcaa tcagtttgcg cggcagtacc cgaccagcgt tacgcatcag taattccagc
    14821 agggcaaatt cttttggttt cagtgttaag ggttcgccag ccagcgtggc gatacgtttg
    14881 ccggggtcga gcatgacgtt accgtggcgc agctcgttgc tggcctggcc gttggttcgg
    14941 cgcatcagag cctccagcct ggcggcgact tctatcaacg caaaaggttt acacagataa
    15001 tcgtcagctc ccagacgcag cccttctaca cgttccgcta acgcatcacg cgcggtcagg
    15061 atcagtaccg gctcacgctg acctttctct cgccattcgc gcaaaatatc gcgaccatcc
    15121 atgcctggta aggttaaatc caggatcacc gcatcataag gtgcgctata aagcgcctct
    15181 tttccctgac gaccttgtgt aaaccagtcg acactaaaac ccattttact aaggccagtt
    15241 ttgatgccgt cgccaatcag catgtcatct tctatcagta aaattcgcat tttttcatcc
    15301 ctgcgataac cgtatttgct gccagtaaac cgtgttgtaa cgcgctctgt acagcattaa
    15361 attttatttt tttcccttaa gaagttgtta aggactatct tgttaaatgc tcggaaacag
    15421 acattaaagg gagtaataaa catgaaaaaa ttcgcagcag taatcgcagt aatggccctg
    15481 tgcagtgcac cggtgatggc agcagagcag ggcggttttt ctggcccatc ggcaacgcaa
    15541 agtcaggccg gaggattcca gaggccgaac ggcagcgtaa cgactgtaga aagcgcaaaa
    15601 tccctgcgtg acgacacctg ggtaaccctg cgcggcaata tcgttgaacg catctctgac
    15661 gatctctacg tgtttaaaga tgccagcggt actatcaatg ttgatatcga ccacaaacgc
    15721 tggaacggtg tgacggtgac gccgaaagat acggttgaga ttcaggggga agtcgataaa
    15781 gactggaatt ctgttgaaat tgacgtcaaa cagatccgca aagtaaatcc gtaattgtta
    15841 ccgctcccgg gacgcgttcc cgggaataat ttcgcaggga ggcaaaatga caaacctgac
    15901 actggatgta aacattatcg atttcccatc aatccctgtg gcgatgttgc cgcaccgctg
    15961 tagccctgag ttgctcaact acagtgtggc gaaatttatc atgtggcgta aagagacggg
    16021 gctttctcct gttaaccaaa gccagacttt tggcgtcgcc tgggacgacc ctgccaccac
    16081 cgcaccgaaa gcgtttcgct ttgatatctg cggcagcgtt agcgaaccga ttcccgataa
    16141 tcgttatggt gtgagcaatg gtgaacttac cggtggacgt tatgccgtgg cccgccacgt
    16201 tggcgagctg gacgatattt cacacacggt atggggcatc attcgccact ggctgcctgc
    16261 aagcggcgag aaaatgcgta aagcaccgat tctgtttcac tacaccaatc ttgccgaagg
    16321 tatgacagaa cagcgactgg aaacggatgt ttatgtgccg ttagcgtgat ggggaatctt
    16381 taccggagtg tgagccaaat cactcgcgat aagtttgagc gaacaatgcc acaggcgtat
    16441 tgttgttggg cttataattt ctccggtaag gaataccatg tatacgcgaa atttattatc
    16501 cggtgcgccg taagccgtct cgggggtatc catgagcaag ccgcacttat ggcaggaggc
    16561 gcggggcact ggtggagaca cagctaaact gggctgaacc gtatacttac agtcgactac
    16621 tttgatgctt taggcttccc tggactcgca taacctcaac ttctcgaatc gcccggagcg
    16681 gacccccatg ccgagtggtg tggcatgggc gcaaccttgt gctgccctct atgccgatca
    16741 agtctacagc aaagagttgt atatcaaggc acattgatgc ctttatcgcc gtcggagcac
    16801 gtccggcggc gctattcacg ctgatctcct gtgactcgac gcggcagata atgtagtatc
    16861 tccggcaata ttgccccttt gaaggctggc gaataagttg aggaatcaga attaatgagc
    16921 gatatggcag agcgccttgc gctacatgaa tttacggaaa acgcctactt aaactactcc
    16981 atgtacgtga tcatggaccg tgcgttgccg tttattggcg atggtcttaa acctgttcag
    17041 cgccgcattg tgtatgcgat gtctgaactg ggcctgaatg ccagcgccaa atttaaaaaa
    17101 tcggcccgta ccgtcggtga cgtactgggt aaataccatc cgcacggcga tagcgcctgt
    17161 tatgaagcga tggtcctgat ggcgcagccg ttctcttacc gttatccgct ggttgatggt
    17221 caggggaact ggggcgcgcc ggacgatccg aaatcgttcg cggcaatgcg ttacaccgaa
    17281 tcccggttgt cgaaatattc cgagctgcta ttgagcgagc tggggcaggg gacggctgac
    17341 tgggtgccaa acttcgacgg cactttgcag gagccgaaaa tgctgcctgc ccgtctgcca
    17401 aacattttgc ttaacggcac caccggtatt gccgtcggca tggcgaccga tattccaccg
    17461 cataacctgc gtgaagtagc tcaggcggca atcgcattaa tcgaccagcc gaaaaccacg
    17521 ctcgatcagc tgctggatat cgtgcagggg ccggattatc cgactgaagc ggaaattatc
    17581 acttcgcgcg ccgagatccg taaaatctac gagaacggac gtggttcagt gcgtatgcgc
    17641 gcggtgtgga agaaagaaga tggcgcggtg gttatcagcg cattgccgca tcaggtttca
    17701 ggtgcgcgcg tactggagca aattgctgcg caaatgcgca acaaaaagct gccgatggtt
    17761 gacgatctgc gcgatgaatc tgaccacgag aacccgaccc gtctggtgat tgtgccgcgt
    17821 tccaaccgcg tggatatgga tcaggtgatg aaccacctct tcgctaccac cgatctggaa
    17881 aagagctatc gcattaacct caatatgatc ggtctggatg gtcgtccggc ggtgaaaaac
    17941 ctgctggaaa tcctctccga atggctggtg ttccgtcgcg ataccgtgcg ccgccgactg
    18001 aactatcgtc tggagaaagt cctcaagcgc ctgcatatcc tggaaggttt gctggtggcg
    18061 tttctcaaca tcgacgaagt gattgagatc attcgtaacg aagatgaacc gaaaccggcg
    18121 ctgatgtcgc ggtttggcct tacggaaacc caggcggaag cgatcctcga actgaaactg
    18181 cgtcatcttg ccaaactgga agagatgaag attcgcggtg agcagagtga gctggaaaaa
    18241 gagcgcgacc agttgcaggg cattttggtt tccgagcgta aaatgaataa cctgctgaag
    18301 aaagaactgc aggcagacgc gcaagcctac ggtgacgatc gtcgttcgcc gttgcaggaa
    18361 cgcgaagaag cgaaagcgat gagcgagcac gacatgctgc cgtctgaacc tgtcaccatt
    18421 gtgctgtcgc agatgggctg ggtacgcagc gctaaaggcc atgatatcga cgcgccgggc
    18481 ctgaattata aagcgggtga tagcttcaaa gcggcggtga aaggtaagag caaccaaccg
    18541 gtagtgtttg ttgattccac cggtcgtagc tatgccatcg acccgattac gctgccgtcg
    18601 gcgcgtggtc agggcgaacc actcaccggc aaattaacgt tgccgcctgg ggcgaccgtt
    18661 gaccatatgc tgatggaaag cgacgatcag aaactgctga tggcttccga tgcgggttac
    18721 ggtttcgtct gcacctttaa cgatctggtg gcgcgtaacc gtgcaggtaa ggctttgatc
    18781 accttaccgg aaaatgccca tgttatgccg ccggtggtga ttgaagatgc ttccgatatg
    18841 ctgctggcaa tcactcaggc aggccgtatg ttgatgttcc cggtaagcga tctgccgcag
    18901 ctgtcgaagg gcaaaggcaa caagattatc aacattccat cggcagaagc cgcgcgtgga
    18961 gaagatggtc tggcgcaatt gtacgttctg ccgccgcaaa gcacgctgac cattcatgtt
    19021 gggaaacgca aaattaaact gcgcccggaa gagttacaga aagtcactgg cgaacgtgga
    19081 cgccgcggta cgttgatgcg cggtttgcag cgtatcgatc gtgttgagat cgactctcct
    19141 cgccgtgcca gcagcggtga tagcgaagag taatatgccg gggagggcct gtttcctccc
    19201 gcttgcaagg aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg
    19261 gaaacgataa aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc
    19321 ttttccagag agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca
    19381 gtctctcaga ccttcagagg ttgttatgct atatatcttt cgtcttatta ttaccgtgat
    19441 ttacagcatc ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc
    19501 gaaacatgtg gccacctttg ggcatatgtt tggccgtctt gcgccgctgt tcggcctgaa
    19561 agttgagtgc cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa
    19621 ccaccagaac aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac
    19681 ggtaggtaaa aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg
    19741 caacttattg atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt
    19801 gaatcatttc aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg
    19861 tggtcgcggc ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt
    19921 cccgattatt cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca
    19981 caacggtctg gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggaaaaga
    20041 tcaggttcgt gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct
    20101 cgataaagaa gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt
    20161 ttgcggggaa cactttcctg cacggtatta ctttacccag ttttacatgg agcaaatatg
    20221 tcactcagtc ggcgtcagtt cattcaggca tcggggattg cactttgtgc cggcgctgtt
    20281 cccctgaagg ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa
    20341 tctcgccgtg ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca
    20401 gggacacgcg cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc
    20461 tggaagggcg acgatgttaa gcttatttac agcaaccgcc tgatagaaaa tgtctcaatg
    20521 acggtggccg ggctacaggt accaggcccg ctgatgggcg gtccggcacg gatgatgtcg
    20581 ccaaacgctg actgggcacc cgtactgccc attcgccaga acgcagctac tctgtggtat
    20641 cacgccaata ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg
    20701 ctggtggaag atgaagtcag caagtcgctg cctatcccca accattatgg tgtggatgat
    20761 tttccggtca ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa
    20821 ccgggaagcg gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac
    20881 gttgaagtct cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc
    20941 tatcaactac agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc
    21001 ctgcctgctc ctgtatcggt gaagcaactt tcgttggcac cgggcgagcg ccgcgagatt
    21061 ctggtggata tgagcaacgg tgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt
    21121 gttgatcgta ttcgtggctt ctttgagcca tccagcattc tggtttctac cctggtgcta
    21181 acgctgcgcc caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg
    21241 ccaactgaaa tcatggccgg ttcgccgatt cgcagtcgcg atatcagtct gggtgatgac
    21301 ccgggtatta atggacaact gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga
    21361 acgtgggaac gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgtg
    21421 atgttccaga tccgtaacgt gaatggtgcg atgccgttcc cggaagacag aggctggaaa
    21481 gataccgttt gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg
    21541 gcgcacttcc cgttctactt caacagtcag acgctggaaa tggcggaccg tggctcgatt
    21601 gggcaactgt tagtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat
    21661 ggaatacttt tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt
    21721 ttatcatttc tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt
    21781 tctcctggcc gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc
    21841 gtgaagagat ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg
    21901 cgtatgtcga tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg
    21961 gctttcgcgt cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc
    22021 tgggtaaacc gaatctgttt ttcggtgtta ctgctggcaa catggattcg atgatcaacc
    22081 gttataccgc cgatcgccgt ttacgtcatg acgatgccta cacgccggat aacgtcgcgg
    22141 gtaagcgccc ggatcgcgcc acactggttt atacccagcg ttgtaaagag gcgtggaaag
    22201 atgtaccggt gatcctcggc ggtattgagg ctagtctgcg tcgtaccgca cattatgatt
    22261 actggtccga taccgtgcgc cgttccgtgc tggtggattc gaaagccgac atgctgatgt
    22321 ttggtaacgg tgagcgtccg ctggtggagg tggcgcaccg tctggcgatg ggcgagccga
    22381 ttagtgaaat ccgcgatgtg cgtaataccg cgattatcgt gaaagaggcc ctgcctggct
    22441 ggagcggcgt ggattccacc cgtcttgata cccctggaaa aatcgaccca atcccgcatc
    22501 cgtatggtga agatttgccg tgcgcggata acaaaccggt ggcaccgaaa aagcaggaag
    22561 ccaaagccgt aaccgtgcag ccaccgcgcc cgaaactgtg ggaaaaaacc tacgtgttgc
    22621 tgccttcttt cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgtattc
    22681 tgcaccacga aaccaaccca ggctgtgccc gcgcattgat gcaaaaacac ggcgaccgct
    22741 atgtgtggat caacccgcct gctattccgc tttctaccga agagatggac agcgtttttg
    22801 cgctgccata caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg
    22861 aaatgatccg tttttcggtc aacattatgc gtggctgctt tggcggctgc tctttctgtt
    22921 ctatcaccga gcacgaaggg cgcattattc agagccgttc cgaagattcg atcattaatg
    22981 agatcgaagc gatccgcgac accgttccag gttttacggg cgtaatttcc gatctcggtg
    23041 ggccaactgc caacatgtat atgttgcgct gcaaatcgcc acgcgctgag caaacctgcc
    23101 gccgtttgtc gtgcgtttat ccggatattt gtccgcacat ggacacgaac caacgatcaa
    23161 cctctatcgc cgtgcgcgtg atctgaaagg cattaaaaag atcctgattg cctctggtgt
    23221 gcgttatgac atcgccgtgg aagatccgcg ctatatcaaa gaactggcga cccatcacgt
    23281 cggcggttat ctgaagattg ccccggaaca taccgaagaa gggccgttat cgaagatgat
    23341 gaagccgggc atgggcagct atgaccgctt taaagagctg ttcgatactt actcaaaaca
    23401 ggcaggtaaa gagcagtatc tgatccccta tttcatctcc gcgcaccccg gtacgcgtga
    23461 tgaagatatg gtgaatctgg cgctgtggct gaaaaagcat cgtttccgtc tcgaccaggt
    23521 acagaacttc tacccatcgc cgctggcgaa ctcaaccacc atgtattaca ccgggaaaaa
    23581 ctcgctggcg aagattggtt ataagagtga agacgtcttc gtaccgaagg gcgacaagca
    23641 gcgtcgtttg cataaagcgt tgttgcgtta ccacgatccg gcaaactggc cgttaatccg
    23701 ccaggcgctg gaagcgatgg gtaaaaagca tctgattggt agccgtcgcg attgcttagt
    23761 gcctgcgcca accattgaag agatgcgtga agctcgtcgc cagaaccgca atacccgtcc
    23821 ggcgttgacg aaacatacgc cgatggcgac ccagcgtcag acgcctgcta cggcaaaaaa
    23881 aagcgtcgtc tacgcaatct cgtccggtga atgctggtgc gaagaaacgg cctaaagcgg
    23941 cggttggacg ttaaattcgc tgttcccggt agcagaattg ctaccgggga tattacatca
    24001 gaatctatct cattaggcat ttatttatat gccgaa
//
LOCUS       DAJTMK010000049        24004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTMK010000049 DAJTMK010000000
VERSION     DAJTMK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24004
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.050"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(85..1035)
                     /locus_tag="OO135_001666"
                     /pseudo
     CDS             complement(85..1035)
                     /locus_tag="OO135_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            complement(1059..1349)
                     /locus_tag="OO135_001667"
     CDS             complement(1059..1349)
                     /locus_tag="OO135_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5397560.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            complement(1608..2489)
                     /gene="yddG"
                     /locus_tag="OO135_001668"
     CDS             complement(1608..2489)
                     /gene="yddG"
                     /locus_tag="OO135_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5397561.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            2721..5768
                     /gene="fdnG"
                     /locus_tag="OO135_001669"
     CDS             2721..5768
                     /gene="fdnG"
                     /locus_tag="OO135_001669"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3306..3308,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5397562.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFVPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            5781..6665
                     /gene="fdnH"
                     /locus_tag="OO135_001670"
     CDS             5781..6665
                     /gene="fdnH"
                     /locus_tag="OO135_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5397563.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            6658..7311
                     /gene="fdnI"
                     /locus_tag="OO135_001671"
     CDS             6658..7311
                     /gene="fdnI"
                     /locus_tag="OO135_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5397564.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(7362..7646)
                     /locus_tag="OO135_001672"
     CDS             complement(7362..7646)
                     /locus_tag="OO135_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5397565.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(7792..8802)
                     /gene="adhP"
                     /locus_tag="OO135_001673"
                     /pseudo
     CDS             complement(7792..8802)
                     /gene="adhP"
                     /locus_tag="OO135_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(8936..10633)
                     /gene="maeA"
                     /locus_tag="OO135_001674"
     CDS             complement(8936..10633)
                     /gene="maeA"
                     /locus_tag="OO135_001674"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5397566.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNDEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(10790..10927)
                     /gene="sra"
                     /locus_tag="OO135_001675"
     CDS             complement(10790..10927)
                     /gene="sra"
                     /locus_tag="OO135_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR5397567.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(11029..11244)
                     /gene="bdm"
                     /locus_tag="OO135_001676"
     CDS             complement(11029..11244)
                     /gene="bdm"
                     /locus_tag="OO135_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR5397568.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11590..12021
                     /gene="osmC"
                     /locus_tag="OO135_001677"
     CDS             11590..12021
                     /gene="osmC"
                     /locus_tag="OO135_001677"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5397569.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(12077..13147)
                     /locus_tag="OO135_001678"
     CDS             complement(12077..13147)
                     /locus_tag="OO135_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5397570.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMRALAEELAVQMGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFFYERCPLATSG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(13144..14040)
                     /locus_tag="OO135_001679"
     CDS             complement(13144..14040)
                     /locus_tag="OO135_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5397571.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIALML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAVHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(14037..14409)
                     /locus_tag="OO135_001680"
                     /pseudo
     CDS             complement(14037..14409)
                     /locus_tag="OO135_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(14411..15961)
                     /locus_tag="OO135_001681"
     CDS             complement(14411..15961)
                     /locus_tag="OO135_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5397572.1"
                     /translation="MKRSIPFRPTLLALVLATTFSVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWSFTLKDN
                     AKFADGTPVTAEAVKLSFERILKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGAYIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16047..16556)
                     /gene="ddpX"
                     /locus_tag="OO135_001682"
     CDS             complement(16047..16556)
                     /gene="ddpX"
                     /locus_tag="OO135_001682"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR5397573.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVVYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDKHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELP"
     gene            complement(16776..19010)
                     /gene="dosP"
                     /locus_tag="OO135_001683"
                     /gene_synonym="pdeO"
     CDS             complement(16776..19010)
                     /gene="dosP"
                     /locus_tag="OO135_001683"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR5397574.1"
                     /translation="MRQDAEVIMKLTDAENAADGIFFPALEQNMMGAVLINENDEVMF
                     FNPAAEKLWGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLE
                     KKDGSKIWTRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVL
                     DPERHIVQCNRAFTEMFGYCMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKT
                     ARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILA
                     AMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSA
                     TIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFD
                     PMTGLPNRNNLHNYLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRF
                     RGKLKPDQYLCRIEGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSI
                     GISYDVGKNRDYLLSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAIS
                     NNQLKLVYQPQIFAETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWV
                     IAEACRQLAEWRSQNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEIT
                     ESMMMEHDTEIFKRIQILRDMGVGLSFLFPPPTRRRNSRLDEQRVTAENLTNSSRPHS
                     RVFI"
     gene            complement(19011..20392)
                     /gene="dosC"
                     /locus_tag="OO135_001684"
                     /pseudo
     CDS             complement(19011..20392)
                     /gene="dosC"
                     /locus_tag="OO135_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(20764..22083)
                     /locus_tag="OO135_001685"
     CDS             complement(20764..22083)
                     /locus_tag="OO135_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5397575.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22214..23749)
                     /gene="gadC"
                     /locus_tag="OO135_001686"
     CDS             complement(22214..23749)
                     /gene="gadC"
                     /locus_tag="OO135_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5397576.1"
                     /translation="MATSVQTGRAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6046 a         5890 c         5962 g         6106 t
ORIGIN      
        1 aagtgctggg gaaaaaaaat taaataacat gagctgcata gtagggcaat tgttcttccc
       61 agaaacattt aaaaaattta agtttttgct ctgttttatt atggggaata aagttccaat
      121 atcggatgta ttattagtat taatgtgggt tattttatta ttgtccagtc ttaaagtttt
      181 gatagatgga agataagtca ggaaatcaag atttatatat ttcaactggt tgttagcagc
      241 gtttaatgtc tgaacattgc agaagttgct atagaatatt ttaatttcag aaagcaaatt
      301 gttgttaata agtaacgtat tcaagttggg aaataagtca ttgtgtaaac ttgttagttt
      361 attatgggat agatccagca gtaagagttc atttttatta cctgcaacaa tatcagttaa
      421 ttgattatgg ctgagattca ggtattgcag ccattcgcaa gattctaatt gcacaaactc
      481 tagttgatta tgtgccgcac taaaataagt aactgatgaa agcctatcta tatcaatatt
      541 tctgagagca ttattattca tagatatatg tgtaatactt acatttctgc cttgaggaaa
      601 atttatagac tcaagagcat tactattcag acttagcttt acgagtctat cgtagttggt
      661 tgcatcaaaa tgtgtgagtt ggttattatc aagattgata gaggtgaagt tttgcagccc
      721 tggtggttga gtggttaaat taagtcctga taaatcaatg gtcacttcat tagtgtcatc
      781 aattatattt cttactgcag tccagtactg catttgtgca agtggatcat tgttgatgcc
      841 gttaactaat acatgccaaa gatgttttga tgatattagt tgatcaatgt cattggttgg
      901 cagtaaattg ttattgctta agtttaaata ttccagtttt gtagtgtttt tggcgtttaa
      961 atgtgcaata tggttgtcgt ttaaagtgat tgttttcatc ctgggatgat tgtgtaatat
     1021 aaggtcagtg atcatattgt gatgggcact taactcaatc agagattctt gccgcgcgag
     1081 ccgttggctt catcgttata gcctttaaaa tcatattccc agtgaccaaa accaatcatt
     1141 tgatcattga tttgcgtttc tcctttaaag ccgagacggg cataagtttt atcaccatcg
     1201 tcacgcttat catcagtaaa ataacgtaga gcggtaacct tgccataaag gtcgagttta
     1261 ttaccatcct tgttatagac ttcggcagcg tgtgctacgt tcgcagctaa caaaccagtt
     1321 acaaccaccg caactatttt taatttcatt taacagaatc cttttaatta tcgttaaacg
     1381 tattttctaa acgaatttta aacggcgtca tttataaatg acatactgtt tttaaaatag
     1441 aatggtgatt aaaaacaatg agaaaaatag atgaaataat attatttatc gatatgtgat
     1501 cgaagtcgaa atgagatata aggtgaatta ctggtatttg aaatttattt tttaatattg
     1561 tcggaattta tctgattaac tgccgggccg tagacccggc gattatttta accacgacgt
     1621 gtcgccagcc agcagagcag ggaaccgccg cagaccatta gcgcgccttg ccagaatgag
     1681 aacgacagcg gggcgctgag cagcacggct gcaagcgctg aggaaagtac gggcgtaaaa
     1741 tacgagccta ccgccattat ggtgacattg ccatgcaata taccgacatt ccatgcagca
     1801 taagcaaatc ctaaggtaaa tgccgcagag atgagtttaa tcatgacggg cgtgctaaat
     1861 accatttctg gttgttgcgt aagaaaatag taaacccaca gacttgctcc cgttagtagg
     1921 acaaaaacgg taattccgtt aaatccgcgt gcgtatttat tcgttactgt gcaataggct
     1981 gcccagataa acgcaccaat aaacgccagg aaataactca atgggctggt ggtgatatta
     2041 ttgatgattt catcataatg taacccattc tcaccgccta acacccagca gacgccgacc
     2101 agggctaata ataatcctgg tacaatcagc cagttggttt tctgaccatt aaacagaatg
     2161 gcaaagagaa ttgtcaggct gggccacaga tagttcacca tacccacttc aatcgcctga
     2221 tgacgggtcg ccgcataccc taaggaaagc gccagacaga tttcatagct gacgaataac
     2281 agactcccgg cgagtaaata gcctttcgga atctgccgaa tacgcggaaa tccaaccgtg
     2341 aagattaaca gcagcccgct taatgaatag atagcagctg cgccgccgac cgggccgagc
     2401 ccctcgctga caccgcgaat caatcctacc atcgtgctcc acaggacgat cgctattagc
     2461 cctatgagcg ttgctttttg tcgtgtcatg ctcgctgttt tgtctctctt gccgttaaaa
     2521 attaagctga attttatagc atttttttaa ctggcctgtc aggcagtggt gcgtttttct
     2581 accgctattg aggcaggtca atttgcgcag gcggattatt ttgtggcaaa cacgtgttct
     2641 ttttgatttc gcgcaaaaag attcagaatt ttactgttag tttcctcgcg cagtaatacc
     2701 cctgaaaaaa gaggaaagca atggacgtca gtcgcagaca attttttaaa atctgcgcgg
     2761 gcggtatggc tggaacaacg gtagcggcat tgggctttgt ccctaagcaa gcactggctc
     2821 aggcgcgaaa ctacaaatta ttacgcgcta aagagatccg taacacctgc acatactgtt
     2881 ccgtaggttg cgggctattg atgtatagcc tgggtgatgg cgcgaaaaac gccagagaag
     2941 cgatttatca cattgaaggt gacccggatc atccggtaag ccgtggtgcg ctgtgcccaa
     3001 aaggggccgg tttgctggat tacgtcaaca gcgaaaaccg tctgcgctac ccggaatatc
     3061 gtgcgccagg ttctgacaaa tggcagcgca ttagctggga agaagcattc tcccgtattg
     3121 caaagctgat gaaagctgac cgtgacgcta actttattga aaagaacgag cagggcgtaa
     3181 cggtaaaccg ttggctttct accggtatgc tgtgtgcctc cggtgccagc aacgaaaccg
     3241 ggatgctgac acagaaattt gcccgctccc tcgggatgct ggcggtagac aaccaggcgc
     3301 gcgtctgaca cggaccaacg gtagcaagtc ttgctccaac atttggtcgc ggtgcgatga
     3361 ccaaccactg ggtggatatc aaaaacgcta acgtcgtaat ggtaatgggc ggtaacgctg
     3421 ctgaagcgca tcccgtcggt ttccgctggg cgatggaagc gaaaaacaac aacgatgcaa
     3481 ccttgatcgc tgtcgatcct cgttttacgc gtaccgcttc tgtggcggat atttacgcac
     3541 ctattcgttc cggtacggac attacgttcc tgtctggcgt tttgcgctac ctgatcgaaa
     3601 acaacaaaat caacgccgaa tacgttaaac attacaccaa cgccagcctg ctggtgcgtg
     3661 atgattttgc tttcgaagac ggcctgttca gcggctacga cgctgaaaaa cgccagtatg
     3721 ataagtcgtc ctggaactat cagttcgatg aaaacggcta tgcaaaacgc gatgaaacac
     3781 tgactcatcc gcgctgtgtg tggaacctgc tgaaagcgca cgtttcccgc tacacgccgg
     3841 acgtcgttga aaacatctgc ggtacgccaa aagccgactt cctgaaagtg tgtgaagtgc
     3901 tggcctccac cagcgcaccg gatcgcacaa ccaccttcct gtacgcgctg ggctggacgc
     3961 agcacaccgt gggtgcgcag aacatccgta ctatggcgat gatccagttg ctgctcggta
     4021 acatgggtat ggccggtggc ggcgtgaacg cattgcgtgg tcactccaac attcaggggc
     4081 tgactgactt aggtctgctc tctaccagcc tgccaggtta tctgacgctt ccgtcagaaa
     4141 aacaggttga tttgcagtcg tatctggaag cgaacacgcc gaaagcgacg ctggctggtc
     4201 aggtgaacta ctggagcaac tatccgaagt tcttcgttag cctgatgaaa tctttctatg
     4261 gcgatgccgc gcagaaagag aacaactggg gctatgactg gctgccgaag tgggaccaga
     4321 cctacgacgt catcaagtat ttcaacatga tggacgaagg caaagtcacc ggttatttct
     4381 gccagggctt taacccggtt gcgtccttcc cggacaaaaa caaagtggtg agctgcctga
     4441 gcaagctgaa gtacatggtg gttatcgatc cgctggtgac tgaaacctct accttctggc
     4501 agaaccacgg cgagtcgaac gatgtcgatc cggcgtctat tcagactgaa gtattccgtc
     4561 tgccttcgac ctgctttgcc gaagaagatg gttctatcgc taactccggt cgctggttgc
     4621 agtggcactg gaaaggtcag gacgcgccgg gcgaagcgcg taacgacggc gaaattctgg
     4681 cgggtatcta ccatcatctg cgcgagctgt accaggccga aggtggtaaa ggcgtagaac
     4741 cgctgatgaa gatgagctgg aactacaagc agccgcacga accgcaatct gacgaagtgg
     4801 ctaaagagaa caacggctac gcgctggaag atctctatga cgctaatggc gtgcttattg
     4861 cgaagaaagg tcagttgctg agtagctttg cgcatctgcg tgatgacggt acaaccgcat
     4921 cttcttgctg gatctacacc ggtagctgga cagagcaggg caaccagatg gctaaccgcg
     4981 ataactccga cccgtccggt ctggggaata cgctgggatg ggcctgggcg tggccgctca
     5041 accgtcgcgt gctgtacaac cgtgcttcgg cggatatcaa cggtaaaccg tgggatccga
     5101 aacggatgct gatccagtgg aacggcagca agtggacggg taacgatatt cctgacttcg
     5161 gcaatgccgc accgggtacg ccaaccgggc cgtttatcat gcagccggaa gggatgggac
     5221 gcctgtttgc catcaacaaa atggcggaag gtccgttccc ggaacactac gagccgattg
     5281 aaacgccgct gggcactaac ccgctgcatc cgaacgtggt gtctaacccg gttgttcgtc
     5341 tgtatgaaca agacgcgctg cggatgggta aaaaagagca gttcccgtat gtgggtacga
     5401 cctatcgtct gaccgagcac ttccacacct ggaccaagca cgcattgctc aacgcaattg
     5461 ctcagccgga acagtttgtg gaaatcagcg aaacgctggc ggcggcgaaa ggcattaata
     5521 atggcgatcg tgtcactgtc tccagcaagc gtggctttat ccgcgcggtg gctgtggtaa
     5581 cgcgtcgtct gaaaccgctg aatgtaaatg gtcagcaggt tgaaacggtg ggtattccaa
     5641 tccactgggg ctttgagggt gtcgcgcgta aaggttatat cgctaacact ctgacgccga
     5701 atgtcggtga tgcaaactcg caaacgccgg aatataaagc gttcttagtc aacatcgaga
     5761 aggcgtaagg gggcgaacag atggctatgg aaacgcagga cattatcaaa aggtccgcaa
     5821 ctaactccat cacgccgcct tctcaggtgc gtgattacaa agcagaagtc gcaaaactta
     5881 tcgacgtttc cacctgtatc ggctgtaaag cctgtcaggt ggcgtgttcg gagtggaacg
     5941 acatccgtga tgaagtgggg cactgcgtcg gggtttacga taaccccgcc gatctgagcg
     6001 ccaagtcctg gacggtgatg cgctttagcg aaaccgaaca gaacggcaag ctggagtggc
     6061 tgatccgtaa agacggctgt atgcactgtg aagatcctgg ctgcctgaag gcgtgcccgt
     6121 ctgctggtgc aatcattcag tacgctaacg ggattgtcga tttccagtcg gaaaactgca
     6181 tcggctgtgg ttactgcatt gccgggtgtc cgtttaatat tccgcgcctc aacaaagagg
     6241 ataaccgggt atataaatgc acgctctgcg tcgatcgcgt cagcgtcggc caggaaccgg
     6301 cttgtgtgaa aacctgtccg accggggcta tccacttcgg caccaagaag gagatgctgg
     6361 agctggcgga acagcgcgtg gcgaaactga aagcgcgtgg ttacgaacat gctggcgtct
     6421 acaacccgga aggggtcggt ggtacgcacg ttatgtacgt gctgcatcac gccgatcagc
     6481 cggagctgta tcacggtctg ccgaaagatc cgaagatcga cacctcggta agcctgtgga
     6541 aaggcgcgtt gaaaccgctg gcagcggctg gctttattgc cacttttgcc gggttgattt
     6601 tccactacat cggtattggc ccgaataagg aagtggacga tgacgaggag gatcatcatg
     6661 agtaagtcga aaatgattgt gcgcaccaaa tttattgatc gcgcctgtca ctggaccgtg
     6721 gtgatttgct tcttcctggt ggcgctgtcc gggatttcgt tcttcttccc gacgctgcaa
     6781 tggctgacgc aaaccttcgg tacgccgcag atgggacgca ttttgcaccc gttcttcggc
     6841 attgcgattt tcgtcgcgct gatgtttatg tttgtgcgtt ttgtgcatca caacatcccg
     6901 gataagaaag atattccgtg gctattgaac attgtcgaag tattaaaagg caatgagcac
     6961 aaagtggcgg atgttggtaa gtacaacgcc gggcaaaaga tgatgttctg gtcgatcatg
     7021 agcatgattt tcgtgctgtt ggtgaccggg gttattatct ggcgtccgta ctttgcgcag
     7081 tacttcccga tgcaggttgt tcgctacagc ctgctgatcc acgcggctgc cggtatcatc
     7141 ctgatccacg ccatcctgat ccatatgtat atggcattct gggtgaaagg atcgattaaa
     7201 gggatgatcg aagggaaggt aagccgtcgc tgggcgaaga aacaccatcc gcgctggtat
     7261 cgtgaaatcg agaaggcaga agcgaaaaaa gagagtgaag aagggatata atctcttttg
     7321 aactttaagc tgaaaatggc gctttaagaa gcgccatttt tttattgcgt aaccagacgg
     7381 cgcagtcgcg acacatccac cgttttttca gcctctgcca gactccaggc gttttgcaga
     7441 ttcagccaca tttgcggcga actgccgatc accacggaaa gtttaattgc catttctggc
     7501 gtcaaagctg cttttccggt cagcaatcgg cttgccgttg agggcgcaat ttccattgct
     7561 ctggcaaact cgcgcaggct gacattaagt tcgtccagtg attcctgaat aatgtccccc
     7621 gggcggggat gattggccat tttcattagt gtctttactc tcagtagcgt tccttctctg
     7681 ggaacactat cattattgtt ccttcctacg caacaataat ccatttagcc atttcatgtt
     7741 ggagagtgtg cgatgctgcg acccgaacat gggagtcgca gcaaagacct cttagtgacg
     7801 gaaatcaatc accatacggc cacggatttt gccttcttcc atctcggtaa agatggtgtt
     7861 gatgtccgct aacggacgca gggcgacttt cggcaccact ttgccttcgg cggcaaactg
     7921 gaaggcttcg gttagatcct ggcgcgtgcc gaccagcgaa ccgaccactt caatgccatc
     7981 cagcacaaga cgtgggatat ccaggctcat agactccggc ggcagaccga cagccacaac
     8041 acgaccgcct gcacggacag catcaactgc cgagttaaac gcagctttag ctactgctgt
     8101 taccaccgca gcgtgagcgc caccggtttt ctcctgcaca atttaggcgg cgtcttcggt
     8161 gcgtgagtta atcgctaaat ctgcacccat ttcggttgcc agttttaact gctcatcatt
     8221 gacatcaatg gcgatcactt tggcgttaaa gacattcttc gcgtattgca gggcgaggtt
     8281 acccagaccg ccaagaccgt agatagcaat ccactgaccg ggacgaattt ttgacagttt
     8341 aacggctttg taggtggtga cccccgcaca ggtaatgctg ctggccgccg ccgagtccag
     8401 accatctggc acttttaccg cgtaatcggc gaccacgatg cactcttccg ccatcccgcc
     8461 atcaacgctg tatccggcat ttttaactga acggcagagc gtttcgttac cactgttaca
     8521 gtattcgcaa tgaccgcatc cttcgtaaaa ccacgccacg ctggcacgat cgcctggttt
     8581 taatgaggtg acacctgggc caacttctgc caccacaccg atgccttcat ggcccagaat
     8641 taccccagtt ttgtcaccaa aatcgccatt cttaacatga agatcggtat gacatacacc
     8701 acaacactcc attttcagca gggcttcgcc atgtttcagt gagcgcagtg ttttatccgt
     8761 aacgtcaaca tgatgatcct tcgtaacaac tgcagccttc atagttcctc cttttcgaat
     8821 gatgttctgc atagcaggtg aggcaaatga gatttattcg ccactaccca gtatggatga
     8881 gatctgaaaa agggagaggg aaatagcccg gtagccttca ctaccgggcg caggcttaga
     8941 tggaggtacg gcggtagtcg cggtattcgg cttgccagaa attatcgtca atggcttgtt
     9001 gcaaagcttc agcagacgtt ttcaccgcca cgccttgctg ctgcgccatt ttgccaaccg
     9061 caaacgcaat tgcgcgggag actttctgaa tatctttcag ttccggtagt accagacctt
     9121 cgtcgttcag caccagcggc gaatactgag caagcgtttc acttgctgac atcagcatct
     9181 catcggtgat acgtgacgcg ccggaagcaa taacacccag cccgatgcca gggaaaataa
     9241 aggcgttgtt acactgggcg atagggtaga ttttatcttt ccataccact ggattaaacg
     9301 ggctgccagt ggcgaccagc gcgttacctt cggtccaggc gataatgtcc tgcggtgtgg
     9361 cttccacgcg tgacgtcggg ttagacagcg gcatcacgat cggacgcgga cagtgtttat
     9421 gcatctcacg gatgatctct tccgtaaaca gcccggtctg tcctgagacg ccaatcagaa
     9481 tatctggttt tacattgcgc accacatcca gcagtgacag cacatcgctg tcggtatccc
     9541 agtcactgag gttttcgcgt ttctgcacca gtttggtctg gaaaggcagc aggttcggca
     9601 tcttgtccgt cagcaggcca aagcgatcga ccataaagac tttctgccgc gccgcttcct
     9661 cgcttaatcc ttcgcgctgg gtctgggcga tgatcatttc ggcaatgccg catcccgctg
     9721 aacctgcacc aaggaagacg atttttttct cgcttaactg accacctgcc gctcggcttg
     9781 ctgcgatcag tgtgccgact gttaccgccg cagtgccctg aatgtcatcg ttaaaagaac
     9841 aaatttcatt gcgatagcgg ttaagtaacg gcatcgcatt tttttgagca aagtcttcaa
     9901 actgcaacag cacgtctggc cagcgttgtt tcacagcctg gataaattca tcaacgaatt
     9961 catagtattc gtcgtcaatg atacgcggat tacgccagcc catatacagc ggatcgttaa
    10021 gcagctgttg gttgttcgtt ccgacatcca gcaccaccgg aagggtatac gccgggctga
    10081 tgccgccaca ggcggtatag agcgacagtt taccgatcgg aatgcccatc ccgccgatgc
    10141 cctggtcacc aagccccaga atacgttcac cgtcagtcac cacaatcact ttgatattat
    10201 ggttcggtac gttttgcaga atatcgtcca tattgtggcg gttctggtaa gagataaaca
    10261 cgccgcgtga acggcggtag atctcagaaa aacgctcaca ggctgcgccg acggttgggg
    10321 tataaataac aggcatcatc tcatcaagat gattgtttac cagacggtag aagagggttt
    10381 cgttggtgtc ctggatgtta cgcaggtaga tgtgtttgtc gatttcggtt ttgaatccct
    10441 gatactggat ccatgctcgt tccgcttgtt cttcgatggt ttcgaccact tccggcagta
    10501 accccagcag gttgaagtta cggcgttctt ccatgctgaa ggcactgcct ttattcaaca
    10561 acggaaattc cagcagtaca gggccagcgt aagggatata aagcgaacgc tgtttttttg
    10621 tttttggttc catgtcactc actctttttt gaatatccat ccctggcacg cttttatcgt
    10681 ctttgcttta ccgccagggc gtcggcccca agtataaagc agataaaaac aaaaacacca
    10741 ttgcgcaggc aatggtgttt aatcgtcatt gaggactgat ggttatgaat tacttttcag
    10801 cggggcgttt tctgccggtt gggttattta ccacgctgga tttgtcacct tcggtaacta
    10861 ttttgcgctg gttagaaatt ttatggtcca gtccaagaat atgacgtgcc tgacggttcg
    10921 atttcatttt aactcctcaa tcctgttgct agttttaagg acaacatcgc cgtagcgaag
    10981 aaacacgtgc taaaccccta aattaggttg ccgatcaagc atagcacctt aaagcgtagg
    11041 gtgctggcca ctgaccacat aattgatcgt ttgctggtag atatcactga ggatgtcgtt
    11101 atcagaagct tccacccatt tggtcagctc catgagaatg tcatcttcag tgacaacacc
    11161 gtgctctgcc cgaaggcctt gctcaatggc attaaccaga gcgggttctg ctgttgaatt
    11221 ttctgcctga taataagtaa acatagtgat tctccgtgtc tgtgtattta tggtgtctgc
    11281 tacggatcgc agatttataa agcacattca gcatggcaaa tatttgccgc ttcgttgtta
    11341 agattagtcc tggttgatga tttttatatt ttaacctcat gatattgata ggtattatta
    11401 ttgtggtgag ttgattaata ttaatgcaat atctcttggg ttatatattc ccggttatct
    11461 attgtggaaa tttaatttaa gtgcagaagt aatatttctg ccggattttt attcggaata
    11521 tcctgcttat cctcgtgctg tttctcacgt agtctataat ttccttttta agcccacatg
    11581 agagcaacaa tgacaatcca taagaaaggt caggcacact gggaaggcga tatcaaacgc
    11641 gggaagggaa cagtatccac cgagagtggc gtgctgaacc aacagccgta tggatttaac
    11701 acgcgttttg aaggcgaaaa aggaactaac cctgaagaac tgattggcgc agcgcatgcc
    11761 gcatgtttct caatggcgct ttcattcatg ctgggggaag cgggatttac gccaacatcg
    11821 attgatacca tcgccgatgt gtcgctggat aaagtggatg ccggttttgc gattacgaaa
    11881 atcgcactga agagtgaagt tgcggtgccg ggtattgatg cctctacctt tgacggcatc
    11941 atccagaaag caaaagcagg atgcccggtc tctcaggtac tgaaagcgga aattacgctg
    12001 gattaccagt tgaaatcgta aagcattgcc ggatgacgtg tcaggcgcgt gaatgcctga
    12061 tgcgttgtta gcatctctac agcgcccgcc agcaccggag ctcacgtccg tcctccctta
    12121 ttgctaatga ttgccggact tcacatccgc tggttgcaag ggggcaacgt tcgtagaaaa
    12181 aacagccttg cggcaacgtg cggtttcccg gcagatccgt tttacgtaat gcccattctt
    12241 cctccagcgg tttatcaatg gcggggaggg aatccagcaa taatcgggtg tatggatgtg
    12301 ccggagccgt tagcacctgt tgcgcgtcgc ccagttctac aatctgcccg agatacatca
    12361 ccgccacccg atcgctcata tgacgtatca ccgagacatt gtgtgaaatc agcacatagg
    12421 tcagcccgtg attttcctgt agcgttacca gtaaattgag gatctgcgcc tgcacggaga
    12481 tatccagcgc agaggttggc tcatcaagca caatcacgtc aggctgcgaa gagagtgctc
    12541 tggcaatggc gatacgttgc cgctgcccgc cggagaacgc atgaggcagg cggtcgagat
    12601 actccggacg aatacccatc tgcacagcca gctcctctgc caacgctcgc attacccgct
    12661 ggcgcatacc acctgaaagc tcaaacggat agcgcgacat aacttccacg gcatccggga
    12721 tttgcatctc ttccagcagg acaatcgctt tagctctggc ttcccgacga cttattggtt
    12781 gatgatggcg gatcacgtcc atcatctgaa gacctattcg acgtgtcgga ttgagggcgg
    12841 tcatcggttc ctgaaagatc atcgccactc gtgcgccgcg ccactgacga agctgttttt
    12901 cccgagcgtt aagaacatcg tctcccagca gtgaaatctg cccccgatgt acgcaataac
    12961 tgcccgtcgg tagcagacgc ataatcagca ttgcggtgac tgatttacct gagccggatt
    13021 ctcccaccag accgacaatt tcaccgcggt taatctgcaa ggacacattg ttgagcgcgt
    13081 gaacatcacc gttaaaaccg gggaaactca aatgcagctg ttgaatgtcc agaacgggct
    13141 gggtcatgac tgctttcctc ctgctttcgg gtcaagcaga tcgcgaatac catcaccaaa
    13201 gagattaaac ccaacggcgg taagcaaaat agctgccccc ggaaatgcgc aataccacca
    13261 ctgatcgagt acatagttgc gaccattcgc taccatcgcg ccccattcag cactcggttg
    13321 ttgagcaccc aggccaataa atcccaacgt ggcggccatt aaaatcgcgc taccgatatc
    13381 cagcgatgcc tgcacgatca gcggcggcag ggagttacgt aaaatatgcc agctgatcag
    13441 atgccaacgc gacgcaccaa aggttttcgc cgcctgaaca taggtatact ggcgtacaac
    13501 taatgcttgc ccccgcgcca ggcgcacata aaagggaatt cgcacaatag caatcgccag
    13561 catggcgtta aataaactcg gcccgagagc ggcagccagt gccattgtca gtaccaacga
    13621 aggaatcgac agcataatgt ccatgatgcg catgatgatg gcgtctgcgc gtccgccaag
    13681 cacaccggat agacatccga gtagcgaacc aatcataccc gcaatggcaa ccacgactaa
    13741 tccggcgaga attgattgct gactgccgac cagtacgcgg ctgaacagat cgcgtcccac
    13801 ttcatcggtg ccaaaccagt gcaccgcaga aggcggcaaa aggcgggcgg ttaaatcaat
    13861 ggcgttggga tcatgcgtcg caagccacgg tgaaaaaaac atcatcaata gcattaatgc
    13921 aatgatgact gcgccggtca cggtgagcgg actgcctttc agcatccata ccagttttgc
    13981 accgttaaat cgcgtttgtt tttgtgggcg tacggcggac gtttcctcgc ttagcatcat
    14041 tcaccacctc cacgtccgat acgcggatca atccacaaat agagcaaatc caccaccagg
    14101 ttgaccagca cataagcaaa tgaaaccacg acggcaaagc ccatcactgc cgggaagtcg
    14161 agcgcctgta ttgatgttac tacccatgca cccattccgg gccaggcaaa aacggtttcg
    14221 gtgagcactg cgccatacaa caaatcgccc agcgccagac ccgatagcat cgctggcgcg
    14281 gtcacccgcc agtaaccgcg ccggatcgcc agggatcaga tgcgagataa taaacgtaat
    14341 cacgcagacg cccgccacca cgagcaccag cccccagcag cgttggcgta aaatactcca
    14401 gaaggtcacg ttatttactc atggtattga tattgaagac ctgttccagc atgggattga
    14461 acacaaagcc tttcacctct ttgttcatcg ccagttggta gtttttctgg aacaggtaca
    14521 cataagcagc gtcatcaatg acgattttct gtgcctgctg gtagtcccgg gtacgctgcg
    14581 tctggtcggt ggtcgcaagc gcattgcgca gtaacttatc gacctcactg ttttcataga
    14641 acgagcggtt acccggcaga ccttttttgt ctgactcaaa ccagtaattc ataaacatat
    14701 acgggtcggc aaaatccgga ctccagttgc caatcgcaat gtcgtaatca cctttaccca
    14761 ctctgtcgcg catggtagcg ttcgccagct tttccagctt cacattgatg cccagcttgt
    14821 tgagactgga ttgtgtcgcc agagcaatag gctcccagtt cggatcctta tcggagtaga
    14881 gaaacgtcag gctggtgggt ttgctcgtca ctttatccca ttcggctttg gctttcgttt
    14941 cgtcatggtt gtattgcatt gccgtcgcat cgtagcccca catgccttcc ggaatcgggc
    15001 cgcgcatctg ttttccgtta ccactcagaa tgccgttaac catgccctga taatcggtag
    15061 accaggaaat ggcccgacgc agatccgcct gattaagagg cgctttgctg ttattcagat
    15121 acaggtaggt gacgcgcagt gacggatact ctgccacatt gactttgttt tcctgcttca
    15181 gggcgttgag ttgatccacc ggcagcgcat cggcaatgtc aatatcacca cgggagagct
    15241 gcaggcgacg ggaggcactt tcaccaataa tttttaccga tactcgctta aagttcggtt
    15301 tattgccagg gtaatgcgga tttggcacca gaactaattg ctgacctttt tgccagcttt
    15361 tcagcataaa tggtccggaa ccggcggtat tttgcgcgag gaagccgcgg gcatcatccg
    15421 ctgcatgttc ctttaacacc gccggattga taatgtatgc accgtcattc gccagcgtgt
    15481 agaggaacgg tgcgaatggc tggctaaggg taaacttcac cgtatgttcg tcgggagcat
    15541 caatctttaa atctttggga aatgcttctg ctggcccctg gccgattttt agtatccgct
    15601 caaaagagag ttttactgct tcggcagtga ccggtgtgcc atcggcaaat ttagcattgt
    15661 ccttcagggt gaacgaccac tctttttgat cgtcagacgc tttccagcta cttgccagat
    15721 cgccttcaac gtcggttgag cctttatcac cgtccgtttt gtactgaacc agccgctgat
    15781 aagacgggta ggtcaccgtc cagtcgttat tatctattgt taccgccggg gcgagggttt
    15841 gtggatcggc ggccttacca atcaccagca tatctttcgg tacggcggcg tgcgcaaccg
    15901 agaaagttgt ggcaaggacg agcgcgagca atgtaggacg aaacggtatc gatctcttca
    15961 tgacagtttc ctgattaact gacgtgctgt gtgccagggg atataaaaca ggtgaattga
    16021 tcagcgagca gtgggtaact cgctgcctag ggtaattcga agtgccacca ttcgctggag
    16081 ataccgacaa aaccgccacc agtcataatc gcattcagca acagccgatt gcgctgagcg
    16141 gcgggcggga cggaagggtg ataggcatgg gagcgctcgt gcatttcatc gaaccctgcg
    16201 cccatatcga ggatgttccc gtgcttatca cgaagcgtca ggtcgatcgc cgtgccacgg
    16261 ctgtgattag aaccgaccgt cacatcaaca acatattgcg ggtctgggca ggcttgccac
    16321 aacatcgcct gtgcttgttg tgggcgatac gcatcgtaaa ccaccagttg taagcctgac
    16381 agctgggcga tactgatact tttcgccagc gcggtaatcg catccttgtg tagcagacaa
    16441 cgcgcttgct gataaatagc tttacctgtg atgttatcag cgcaggcgta tttcaattcg
    16501 atctccagat cagggaagat tacggctaaa tcaaccagtt cggtggtatc cgacataaac
    16561 ggttcctgta taagacaaaa attgctgcgc tttccgctta tgcagatctc atgccatgcc
    16621 gggataagcg ccagaatgct ggcttaaagt tatatttttt aacttttgat caacatttgt
    16681 gcagcgtagt gcagttttgg tgcaagaggg gaagttaagg aaggaatctc ccggaatcgt
    16741 agctgaaatc acagtattta agtgacggtg tcacgttaaa tgaaaacccg cgagtgcggg
    16801 cgagaggaat ttgtcagatt ttcagcggta acacgctgct catccagcct ggaatttctt
    16861 cggcgggtag ggggcgggaa aagaaatgat aagcccacgc ccatatcacg caggatctga
    16921 atgcgcttaa agatttcggt atcgtgttcc atcatcatgc tttccgtgat ttctaccgtc
    16981 agctggtggc cgtcaatacc ccaggcgtgc attgcatcag acacctgatt aggcagttga
    17041 ttactgcgaa agtgcagtgc cgacaagtta acggaaagcg ccgggatatg aatattctgg
    17101 ctacgccatt ctgctaactg acggcaagct tccgcgatga cccagcgccc aatattttcg
    17161 atttcaccaa tctcttctgc gagaggaata aaccgtgaag ggggcacatg accatgctgg
    17221 ggatcgtgcc agcgcgcaag ggcttcgatg ccgtacagtt cacccgtttc tgcgaagatt
    17281 tgcggctggt aaacaagttt cagttggtta ttgctaatcg cttctttcag cgctgcgcct
    17341 aaaaccaaac gctcttttac catttcgttc atcgccgggc tgaagaactg ccagccgtta
    17401 ccgccattct tgcgaatata atctattgca ttgtgagcag tggagagtag gtaatcgcgg
    17461 tttttaccca cgtcgtagct gatgccaata ctcaaggtaa gcgggaatgg tttatcgtca
    17521 atcattatcg gcttgctgac cacattccgt agctcatcgg cgatctgggt aatgttactg
    17581 acgtcgtttt ccaggctcac gaggacaaac tgcgtacctt cgatacgaca gagatactga
    17641 tccggtttga gttttccacg aaagcgattg accacttcca gcaatgcctg atcggcccac
    17701 gcatagccaa ggctatcaat cacatcctga atatggtcaa caccgatgag atacaccacc
    17761 ggagagacgg ctttgtcgac caggtcatcg aggtaattgt gcaggttatt gcgatttggc
    17821 agaccggtca tcggatcaaa ttggatgagt tgttcaatat gctggcggct tttttcctgt
    17881 tccagcgcca gcgcggccat atgctgactg atatctgcca cgcgttcgat aaaggcgctg
    17941 gtttctgctc ctgacgaggt tttaatttgc aggatccccg caggcgcgcc atcacgctga
    18001 cgaatggtcg ctgaccagct ttgcgcattt tgaacttctg caccgtgcga agatgacgcc
    18061 cagtgtatcg gcatcccgtt gcgcagtgcg aacagcgaaa catgcgattc gttgagaaca
    18121 gattcgatgt tacgacaaat gatttccccc atttcatgaa atggcgggct gctgcacatg
    18181 gcggcgagaa tattgccttc aagctggcgg atttgccgtt cttcggtgat atccgagaaa
    18241 gtcattacca ggttctgcag atgcgcgagc acgtcataaa ccgggctgat agaggcttta
    18301 atccagattt tttcaccggt gcgcgtcaac agcagaaatt cgtcctgatc gcgggcggtt
    18361 ttccatagca actgttgtaa acgaatgcgg ttatcggcag ggaattcagg aatgttcagg
    18421 agtgtatcgg gctgcatacc gctggcttcg ctaatgcagt aaccaaacat gcagtaacca
    18481 aacatttcgg taaatgcgcg attgcactgc acaatatggc gttccggatc gaggacaatc
    18541 accggtcggt cgagatggtc aacggcaata atcaattgtc gggtctgttc tttttgcgcc
    18601 atttctacgc tggcatcccg taccagcgcc aggtaataaa ctttcccctc ggcgctcact
    18661 ttcgatagcg caaaacgggt ccagatttta ctgccgtctt ttttctccag ctgcagctcc
    18721 cgactcatcc cctcgacgcg cgctttacca ccttcacggt tatgacgaat gtattcagga
    18781 tgcgcaggac gcaaatcccg cggaatcagc atatcaatgt tattgccaat gacttcttca
    18841 cgtttgtacc cccagagctt ctctgcggcg gggttgaaaa acatcacttc atcattttcg
    18901 ttaattaaca ccgcgcccat catattttgt tcaagggcgg ggaaaaaaat gccatcggcg
    18961 gcattttccg catcggttag cttcatgatt acctctgcat cctggcgcat ctaaagactg
    19021 gctttccaga gttcaacacg gtttctacct cgtcttttgg cgatatacag agcttcatcg
    19081 gctatttgaa tgaggcgctc atagtcagga tgaccattaa acatggcggc accgatggaa
    19141 agtgagaggg caatatcctc gccgtttgcg gctttcagtt tggttttctc cacccgactg
    19201 cgaatacgtt ctgcggtacg taacgtttcg ttttcagaag cttcagtcaa aacaatgata
    19261 aattcatcgc ccccgtagcg aaaaacataa tcactactgc ggacgttgtc ataaaaggcc
    19321 tgagaggctt tacgcagaat ttcatcgcca gtgttatggc cccacgtatc gttgatctct
    19381 ttgaatttat caacgtcaat aatcagcact gacagcggtg taccggtccg gttggcatgg
    19441 gcaatttcgt gtttgaagat agtcggcagg aagcggcggt taagtaattt cgtcagtaca
    19501 tccataccga cttcgtggcg cgatacttct tcaaacaatt cacgcagcaa ggtaataatt
    19561 tgcgatacag tatttcttat ctgtaataaa aatttcaccc gcagacttct gttattcaaa
    19621 ttcctggtgt tacgcatggt ttgattgaat ataccgtcga aatcctgaat cagacgggag
    19681 atatgcctac ttcggcaatg ccactaaaat aatgtcgacc tttatggtta aaccacaggc
    19741 caaaatcagc ctggcttaaa ggcaaactac tgcctaaatc agaatccagc aggattttat
    19801 agataatatc tatttcccat gaaagtattg aggctatttg ccgttctttt tcttcttcgg
    19861 cgttttctag taacgagaag atacgatagt tttcatcttc ctttgaggca ctactgtcac
    19921 taaaggtaaa cgcgcgggtc atcacttcca tagcgatatc aatactgtta atcgagaaat
    19981 ggtagacctg aagtttttct gcggcagaat aatccgaaga gaagattacc ggatagagga
    20041 tttttttcag cacccgaaac cccatctcga caatttctac cggaattcct atgcgggcat
    20101 gcacttccgc gacggtatgc tggatttgta ttagcctttc gacatcgtca acttgggcag
    20161 aaagcacgtt aataatccag cgttccatcg tacttttcaa ctgccgctca acttgttcat
    20221 tactcaagaa ttcttcggca tgcgggtcga tgcggacaat tcgataaaac tcgatactca
    20281 gataatgagc atgcgcaacg gcaatttccg cggctttagc acgaatgagt ggatctgcct
    20341 gttcgacaag tccggtccac tcatctttca ttcttttaaa atacatctcc ataattcaca
    20401 cccttataag gctgggaaat cagacggaat caaaacgcaa cgtgcgagat cgactaactg
    20461 caccatattc tcctgaaata tgaagatata ctgaaaagaa ataagcgact taggacagtt
    20521 tcaatctacg ctactgttct tcagaagagt atagcccatc gtaattattt ttcggtgaca
    20581 gcgaatatcg tatggttttt catattcata catttttatt agggatttat agctgtttaa
    20641 ctaagtgtgg ttaattgcgt ggttaatttg acttatgtaa gcatgattac tagtgggata
    20701 tttcacaagg tgtaacaagc cggtgggtaa agcaccggct tgttacaaag taagcatggg
    20761 agtttaactg ccccaacgac tttgcagata gctgaccgct tgttgagtct gcggtttatt
    20821 cagatagtcc tcacggaaca agatggtgcc gctaatttct ggtaccgcat cgttaagatc
    20881 gagctgcttt ttcagttccg gtacgccgcc gttaatcatc cagtctggct ctatctttga
    20941 aggttcaccc actttataga aggcgatacc gatatacagg cgggtcctgg tcggtttaac
    21001 gacatccgcc caccattttg ccaacacgtc ataacgcgcg gcactccgtg agaacggcca
    21061 gtaaatttgg ggagcaatgt aatccagcaa tccttgttcc acccatcgac gggtgtcagc
    21121 gtaggattcg tcataggctg ccgcgcctcg ggtatcggaa ccgagcggat cgtgtgatcg
    21181 gttacgccac acgcctgccg ggctaacgcc aaattcgact cccggcttaa tgcttttaat
    21241 ggtgtgcgat acttttgtaa ttaactgctg agtattgttg cgccgccagt ctgcttttga
    21301 cgcaaatgcg cctccgtatt tacggtacgt ttcgttatca tttagccgtg aacccggtga
    21361 ctccgtatag aaatagtcgt caaactgcac gccatctacc ggatagcggg aaaccacttc
    21421 ggcgactatt gatgtgatcc agtcctgaac ctcagggatg cccgggtcga ggacaaagcg
    21481 atcgccagac gttctgatcc agtcacggtg ttgcacatag acgctcgccg gttgttgaga
    21541 cagagtgcta ttcagttccc tgatagtacc gggcttcgta ttaaccgata cgcgataggg
    21601 gttaaaccag gcgtgtactt tcatcccacg cttgtgggct tcgtcgagca taaattgcag
    21661 cggatcgtaa cccggatttt caccaatctt accggtcata agatcggacc acggcaaaat
    21721 tttcgatggc cacagggcgg ttccgtccgg cttgacttgg aaaaagaccg tgtttatacc
    21781 gagacgttgc agatgatcca gtttgtcgat catcgcctgt tgttgtacac gggcccggct
    21841 ggtggggtta ctaatgttaa ccgaggaaac cggtggccag tcgaggcgag acaccgtggc
    21901 cagccagatg ccacgcatcg gttgtgacga ttgttgcgtc gtggctggtg gctttgaacc
    21961 tgctggtggt gtcaccatgg actctggagg cgtgctttta caactacaca gtaaaagtgc
    22021 cagtgcaacc agtatcgctg gtcttctaat cgttaatttc ttgtttcggg agcagatatc
    22081 catatgtgct ggtttccggt aaacagatgt gcgctcattc tcgtacttat cccggttaag
    22141 tcaatacgac agcaagcacg aaaaagggag cgatgaatta tcgctccctt gtcttataac
    22201 cattcagaca tggttagtgt ttcttgtcat tcatcacaat atagtgtggt gaacgtgcac
    22261 gcgggtgcag gaagaagtga ccttttggtg cgttctgact gttgattggc tccagagtga
    22321 cgccggtatt cgctttgcct ttacgatcat gaacagcata gagaataaag ggcagggcaa
    22381 gtaccaccag gaaactaacg accagtaatt caacatacat atcggtagag tcaccctgga
    22441 tgttatccgg cggcaggaag gaaacaataa acgccataat tgaagtcagc agaccgacaa
    22501 ttgccacgac cagtttcacc cctttaccac cagggatatt aaatgtgcgt tttaagtcag
    22561 gatgtttaag aaccaacaca atgtagccaa taaacagcat gaaataagca cacagataaa
    22621 tcaccaccgt cagtgccagt gcgatcagga aggacatgtt gttaccgcca ccggtattgg
    22681 tgaggatgat caacgcgata gaagtaatca ccagctgcga aatgaccagc gttaccggta
    22741 cgccattttt gttcatttta gcgaatgccg ctggcagcag gtttttctgc gctgtgacat
    22801 acatcccgcg agaaggacca acaatccagg aggcgatttc cgccagaaca cccagcaaca
    22861 gcagtgcgga gatcacgcga accgtccact caatttccgg tgccacatgg gacatcagaa
    22921 cggtaaaggt ttgcattacc cctgcggaga ggttgatttc attacccgga atgaccatcg
    22981 caatagataa accgccaaca gagcttaagc agattgccgc caccatcagc agtaacatag
    23041 ccagtggata gtcgcgccca gggttgctca tttcattgac gtgagttgcg gaagcttcta
    23101 cgcccatata actcaaaatg aaggcaacaa atacaaccag tgtgcccact ttagagaagt
    23161 cagggaagaa ggtcttcgaa tccatttcga tagcaacggg ggcaccggag tgcagataaa
    23221 tagccgctaa tgcgatcaaa ataaatgcag gtaacaggat accggcgaag aagccaactt
    23281 tagcaattcg cgccgtgtat ttcgtgccac caaactgcgt taatgccagc gcccaaagaa
    23341 tgatgagtgc tgcaatagtt ttggtaatgg ggtcttcatt cagcgctggc catttcagga
    23401 tgtaggagag tgcccctaac acgaaataga gcatcggaat aaaaccaatg gcgatttgca
    23461 gatagccaaa tgagatcgct gcaaatcccc atctcggccc cagagtattt gatacccagg
    23521 cgaagacacc accttcttcc cagccgtcga cggtggccat ttccgcagca caaagtccca
    23581 cgggaataaa ccataaaatc ccgcctaata gcaggaagaa gactaatgaa aagcccgatg
    23641 ttgcgaaggt agggtattca taaacagcca ttaccatcga tgccgttatg gcaaagaatc
    23701 cgagtaatgt gagctgctta gctctacctg tctgtactga tgtagccata ttatccccct
    23761 aaaacggtat tcctgtcgga accgcacctg ttttgttctg aagcgtattc agaacaatat
    23821 tttccgttgc taatgccagt gaacagactt tggaaattgt cccgaaacgg gttcgtttcg
    23881 ggacaccgtt accgttaaac gttatcaggt atgtttaaag ctgttctgct gggcaatacc
    23941 ctgcagtttc gggtgatcgc tgagatattt cagggaggct ttgtagtctt ccagcaacag
    24001 ttca
//
LOCUS       DAJTMK010000050        23985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTMK010000050 DAJTMK010000000
VERSION     DAJTMK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23985
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.051"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            113..719
                     /locus_tag="OO135_001687"
                     /pseudo
     CDS             113..719
                     /locus_tag="OO135_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            822..1325
                     /locus_tag="OO135_001688"
     CDS             822..1325
                     /locus_tag="OO135_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCR5397577.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTPAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSKLRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1392..2849)
                     /gene="nuoN"
                     /locus_tag="OO135_001689"
     CDS             complement(1392..2849)
                     /gene="nuoN"
                     /locus_tag="OO135_001689"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5397578.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2856..4385)
                     /gene="nuoM"
                     /locus_tag="OO135_001690"
     CDS             complement(2856..4385)
                     /gene="nuoM"
                     /locus_tag="OO135_001690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5397579.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="OO135_001691"
     CDS             complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="OO135_001691"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5397580.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="OO135_001692"
     CDS             complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="OO135_001692"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5397581.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="OO135_001693"
     CDS             complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="OO135_001693"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5397582.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="OO135_001694"
     CDS             complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="OO135_001694"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5397583.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="OO135_001695"
     CDS             complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="OO135_001695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5397584.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="OO135_001696"
     CDS             complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="OO135_001696"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5397585.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="OO135_001697"
     CDS             complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="OO135_001697"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5397586.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="OO135_001698"
     CDS             complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="OO135_001698"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5397587.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="OO135_001699"
     CDS             complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="OO135_001699"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5397588.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="OO135_001700"
     CDS             complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="OO135_001700"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5397589.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="OO135_001701"
     CDS             complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="OO135_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5397590.1"
                     /translation="MSMSTTTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17046..17984)
                     /gene="lrhA"
                     /locus_tag="OO135_001702"
     CDS             complement(17046..17984)
                     /gene="lrhA"
                     /locus_tag="OO135_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5397591.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18904..20121
                     /gene="alaA"
                     /locus_tag="OO135_001703"
     CDS             18904..20121
                     /gene="alaA"
                     /locus_tag="OO135_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5397592.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20205..20804
                     /gene="yfbR"
                     /locus_tag="OO135_001704"
     CDS             20205..20804
                     /gene="yfbR"
                     /locus_tag="OO135_001704"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5397593.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20863..22695)
                     /locus_tag="OO135_001705"
     CDS             complement(20863..22695)
                     /locus_tag="OO135_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5397594.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22782..23432)
                     /gene="hxpA"
                     /locus_tag="OO135_001706"
     CDS             complement(22782..23432)
                     /gene="hxpA"
                     /locus_tag="OO135_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5397595.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23443..23937)
                     /locus_tag="OO135_001707"
     CDS             complement(23443..23937)
                     /locus_tag="OO135_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5397596.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
BASE COUNT         6146 a         6614 c         5994 g         5231 t
ORIGIN      
        1 ttgtatagat ctaacaaccg taataaaaca aatactcata actcattata aataatgata
       61 aaataacgtc ttttattgat aatttatata tgaaaaaaat tccttttgtt atattgtttc
      121 tgatttcact ccctctgatt atttttacca accctgggtc actgcattac caccaacgcc
      181 ccgacacgtc agtcctgagc aattagaaaa aacagttcgt tatcttacac aaactgtgca
      241 tccacgtaat gccgacaata ttgataatct gaataggtcc gtagagtata taaaagagtt
      301 ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc tattacgact
      361 ctgcgcctga tagccaggat tacctttacc cgcaatgtcc tggctttatc ccgatcgggg
      421 ggactttatt gcctggtcgg cagaatacag gatatcaacg ccgttcgtca ggtaaaaacg
      481 gcattgttat catcccggga tttatctgtt tattctatga atgccccatg gtttattccc
      541 ggtattgatt tctctgacca cctgaattat tggcaacacg atattcccgc cgtaatgatt
      601 actgacaccg ctttttatcg taataaacaa taccacttgc ccggtgatac agcagacaga
      661 ttgaattatc agaaaatggc tcagatggtg ttataacctt gttacacaac agtaaataat
      721 aaaaatttct cgaggcgggt ccagaaacag cgacatgtac attttatttt ccctcgtctg
      781 ttttaagatg cgaaacataa aattcaatgg aggaaaaata aatgggaatg attggttatt
      841 ttgcagaaat tgactcagaa aagattaacc aactgcttga gtctactgaa aaacctttga
      901 tggataatat acatgacacg ctttcaggat tacgccgact ggatattgat aaacgctggg
      961 attttttaca ctttgggctg acaggcaccc ctgcttttga tcccgcgaag aatgatccgc
     1021 tgagtcgtgc cgtgcttggt gaacacagcc tcgaagatgg catagatggc ttcctgggat
     1081 tgacctggaa tcaggaatta gccgcgacaa tcgaccgact ggaatcactg gatcgaagta
     1141 agctgcgtaa acagttttcc attaagcgac tcaatgaaat ggagatttat ccgggagtga
     1201 ccttttcaga agagttagag gggcaactct ttgcctcgat catgcttgat atggaaaagc
     1261 tgattagcgc gtatcgacgt atgctgcgac aagggaatca tgcgctgacg gttattgttg
     1321 ggtgatggtg tcagagcgcc aaaaaaaacc gccgaacatg tcggcggctt tctgacttac
     1381 aaactaacag attacatcag cggcattgct aaccgcacaa tactaatcag cggttgtggc
     1441 catacaccca gcaccagtac caacagtgca gaaatcagca ccacgatacc gcccgcgctg
     1501 tactgccagt ttgacggtgc atcgcgaccc ggttgttccg gtgcgtgcag gtacaggctc
     1561 accgccacgc gcaggtagta gtagaggccg attgccgaac cgacaaccac ggcacccacc
     1621 agccaccaca agtgtgcctg gacaccgacc gccagcacgt agaacttacc gataaagccc
     1681 agcgtcatcg ggataccggc cagagacagc atcatcaccg tcatcactgc cgcgaggatc
     1741 ggacgatgcc agaacagacc gcggtaagag aacagggaat cagcatccgg gccacgatac
     1801 gggctggaca tcaggctgac cacgccgaac gcgccgaggc tgctgaacag ataaccggcc
     1861 aggtaaaccc ctaccgcttc catcgacatc tcgccggttt gcagcgcaat cagcgccacc
     1921 agcagatagc cgaggtgaga gatagatgag taaccgagca gacgtttgat gttggtctgg
     1981 ctcaacgcca tcaggttacc gaagatgatg gaggcaaagg cgataatcgc cagtactacg
     2041 cgaatcgctt cgctgtcacc caccggtgca tagaggaaca gacgcatcac cacaccgaag
     2101 atagcgattt tgctcgccgt cgccaggaaa gtggaaaccg gcgcaggcgc gccctggtat
     2161 acgtctggcg tccacaggtg gaacggcacc agagagagtt tgaagccgag gccaacaatc
     2221 atcatgccga aacctgccag caacagcggc tcgttgagca taccgtcgcc gaggttttta
     2281 cccagcgcga caaacgacag gtcgccagac tgcgcataca ccagcgccat accgaacagc
     2341 aggaaagaag acgctgcggc agaaagaatg gtgtatttga tactggcttc cagcgaacgt
     2401 ttctggcgga aagcgtaacc gaccaggcca aacagcggca aagagatcag ttcaataccg
     2461 aggaacagag acgccagatg gtttgcattc gccagcagga tcccgcccag cgcggcaatt
     2521 aacaccaaca ggtagaactc atccttgttg tcgttatagc cttcaagcca cgggtaggcg
     2581 aaagtacagg tggcgaggct cgccaataat accagcccgg tgtaaagcat ggcgaaacca
     2641 tcaacgcgca tcagcggcgt aacgtccata gcgcccgcct ggccaacaaa ccagagcgaa
     2701 accagcgccg cgttaagccc aataaccgag agcgtagcgt tgaggaaatg attgcgtcgc
     2761 cacgcaatgg agagcatcac aaccaccacc gttaagccga cgatcagcaa cggtagcagt
     2821 gcgatcaggt tttgtggagt tattgtcatg gcgatttacg gccttgtagt agtaacggaa
     2881 ttaacaaacc actgctggat attgccaatc gcggagtgcg aggtatccag aatcggctgc
     2941 ggatagaagc ccagcagtac cagcagcacc accagcaaca ggatcataaa cagctcacgc
     3001 agcgacatcc ctggcagttc ctggctggca atctggcttt tcgctttacc gaagtaagcg
     3061 cgatgcaaca tcgccagcga ataaacagat gcaaagacca gcccaaaggt agagataacg
     3121 gtaatcaccg ggacaacctg gaagctgccg aacagaatca taaattcgcc gacgaagtta
     3181 ccggtgccag gcatcccaag cgttgccacc gcaaagaaca gcgacaatgc tggcagccat
     3241 ttcatcttgc tccacagacc gcccatcatg cgcatgtctc gggtatggat acgttcataa
     3301 agctgaccac aaagaataaa cagacccgcc gccgacaaac cgtgcgcaat catctggatt
     3361 accgcgccct ggtaggccaa ctggctgccg gtgtagatag caatcagcac gaagcccatg
     3421 tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg tctgggcgaa ggccatccac
     3481 gcaccgtaga agatgccgat aacacccagc cacatagcaa ttggcgcgaa ctctgccgac
     3541 gcgttcggga acagcggcag ggagaaacgc agcaaaccgt aagcggcagt tttcagcaag
     3601 atccccgcga ggtcaacgga accggcggtc ggagcctggg agtgcgcatc cggcagccag
     3661 ccatgcagcg gaaccaccgg cattttgact gcgaaggcga tgaagaagcc cagcattaac
     3721 aggtattcca caccactgga cattggcgta ttcagcagct cttcatagtt gaaggtccag
     3781 acgccggtcg cattgtagtg aacaaaaacc agcgccagga tggcaatcaa catcaccagg
     3841 ccactcgcct gggtgtaaat gaagaacttg gttgccgccg tgatacgcgt tttaccgtca
     3901 gaggctttat gcccccacag tgcgatcagg aagtacatcg gcaccagcat catttcccag
     3961 aagaagaaga acaggaacat gtcgatggca aggaacacgc cgataacgcc gcccaggatc
     4021 cacatcaggt tgaggtggaa gaagccctga tatttttcga tctctttcca cgaacagagt
     4081 accgccagca caccgagcag accggtcagc acgaccatca gcagcgacag accgtcaatg
     4141 gcgagatgaa tagagatacc aaaacgcggg atccacggca tgtcgaattc agactgccac
     4201 tgcggaattc cggcggattg cgtcagtgaa taaccgccct gcaaccacag ttgcagcgac
     4261 agcgccagcg tcaatcccat ggtgatcagc gcgatccagc gtggcacctt gacgtcaaag
     4321 cgttcggtct gccagcacag gaagccgcca ataaagggaa ttaatattag ccagggtagt
     4381 aacatggcga tctttattcc ttgtaaaagt cccgtcagga ccggattttc aacgaattct
     4441 cacgacagaa aattctcttc ggactggggg tagtgtcgga tgcgccgcga aggcagctta
     4501 tccgacctac gttcttatat gcccaggggc aatcccacaa tccttaactc aacgcagtac
     4561 catcaacagt gccagcacca cgaccgcacc gatgctcatg gatgccacat accagcgcag
     4621 ataaccgttc tcgcttaaca gcaggccttt acctgcaaag cgggaaagaa ccgccggaat
     4681 gttcatcatt gagttcagcg gatcgcgttt cagcaaccag gcaataccca ggaacggctt
     4741 gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg ttgtaccacc aggtgcccag
     4801 cagacggccc ggcgcactgt tggcaatgga ggtcaccaga gtacgtttac cctgccacag
     4861 ccaggctgcc agcagaatgc cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa
     4921 catgctgccg tgcgccagtt ccgtcgtttg cggaagcacg ctctgcagcg gcggtacaat
     4981 cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc agcggcaggc tgtgagttac
     5041 ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg aagacgatga aaatcatacg
     5101 gaaggtgtag agcgaggtca taaacgcacc gaccagacct gccaccatca gattgatatg
     5161 accattcgcc atcgcacccg cgaggatctc atccttactg aagaagcccg cagtgaccag
     5221 cggtagtgcc gacagtgctg cgccgcccac caggaagcag aaataaacca gcggaataga
     5281 tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga tggcaggcca gaatgacgga
     5341 accggatgcc aggaacagca gcgctttaaa gaacgcgtga gtcatcaagt ggaaaatcgc
     5401 cgcatcccat gcctgcacgc caagcgcgag gaacatgtag ccaatctggc tcatggtaga
     5461 gtaagcgaga acacgtttga tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag
     5521 cgtaaccgcc ccgacaatgc ccaccagatg cagaatttcc ggcgtcatca ggaacaggcc
     5581 gtgggtacgg gcgatcaggt agacacccgc ggttaccatg gttgcggcgt ggatcagtgc
     5641 ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat gtctgcaacg gcaactgcgc
     5701 agatttaccg accgcaccgc ccagcagcat cagcgtcgcc cacatcagca tgttattgcc
     5761 gtcagcaaag tgcgctggtg ccagttccac catttcgcgg aagttcaggg tgcccagttc
     5821 gttgtaaaga atgaacagcg cgaaagcgag gaacacgtca cccacacggg tcacgacgaa
     5881 cgctttcatt gccgctgcgc cattcttcgg atcggtgtaa tagaacccga tcagcagata
     5941 ggagcacagg cccacgcctt cccagccgag gtacatcagc agcaggttat cggcgagaac
     6001 cagaactacc atgctggcga tgaacaggtt ggtgtaggcg aagaagcgag agtagccctc
     6061 ttcaccgcgc atgtaccagg aggcgtacat gtgaataagg aaacccacac cagtgaccac
     6121 cgagagcatg gtcagcgaca ggccgtccag caccaggtta aaaccgatgt taaagtcgcc
     6181 taccgacatc cacgtccaca gcggctggct gtatgcctgc tcgccgttag cgaagaaatc
     6241 aacgccgata aaggcggtta ccagcgccgc caggcccaca gagccaacgc cgacgatcgc
     6301 cgaaacgttt tcagaccagc gcccacggga gaacgccagc aggacgaagc caatcaatgg
     6361 caaaataatg gttaaggcaa gcatgttcat ccgcgcatct cacttactga atcgatgttc
     6421 aggttctggc gacgacggtg aagttgcagc agcagcgcaa ggccgatact cgcttctgcc
     6481 gccgcgaggc tgatggcgag aatgtacatc acctgaccgt cggtctgacc ccagtagctt
     6541 ccggcgacca caaaggccag cgcggaggcg ttaatcatga tttccagacc aatcagcata
     6601 aacagcagat tgcgacggat aaccagaccg gttaagccaa gaacgaataa gattgccgag
     6661 aggatcagtc catgttgtaa ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt
     6721 cgtctttacg attgctcagc acttcacccg cacgctcttc acgaccgacg tggaaggcca
     6781 caaccagacc cgcgagcagc agcatagaag ccagttccac cgccagtacg taaggcccga
     6841 acagcgtaat acccactgct ttagcactga ttggcgtacc gtcgataccc tgatcattaa
     6901 caccaaggat ggcgtaaaca atcaccacca gcatgatggc cgacaaaatt gccggaccaa
     6961 tccacacctg cggtttcagc cactggcgtt cctgttcgat ttctgaaccg cccaggttga
     7021 gcatcatcac cacgaacacg aacagcacca taatggcacc cgcgtagacg ataatttcca
     7081 gcgcaccggc gaagtaagcg cccagtgaga agaacacccc ggaaatcgcc agcagcgaaa
     7141 taatcaggta cagcagtgcg tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa
     7201 gtatggctat caggccacag atataaaaag cgaactccat tgcccctctc cttacggtaa
     7261 caggctcttg acgtcgatag gcttggcttc gttctctgct tcgcccttat ctttgccgtc
     7321 gattgccata cctgccatcc ggtagaagtt atattccggg tatttgcccg gaccggagat
     7381 cagcagatcc tctttttcgt aaaccagatc ctggcgcttg tattccccca tttcgaaatc
     7441 cggggttaac tgaatcgccg tggtcggaca ggcttcttcg cacagaccac agaaaatgca
     7501 gcgtgagaag ttgatgcgga aaaattccgg gtaccagcga ccgtctttgg tttctgcttt
     7561 ttgcagcgag atacagccga ccgggcaggc taccgcgcag aggttacagg ctacgcaacg
     7621 ctcttcgccg tccgggtcgc gggtcagaac gatacgacca cgataacggg gcggcagata
     7681 gaccggctct tccgggtaca ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat
     7741 ccagatacta cgaacctggg tgccgaaacc tactaacaat tcttttaagg tcatggtctt
     7801 attgcccctt attgcgcctg ccagagaatg acagccgccg ttaccagcaa gttgatcagc
     7861 gtcagcggca ggcagatttt ccagccgaag gacattacct ggtcataacg cggacgcggt
     7921 aacgacgcac gaatcaaaat gaacatcatc ataaagaacg cggttttcag cgcgaaccag
     7981 atgaatggcg gtaacaacgg gccttgccag ccaccgaaga acagcgtcac catcaatgca
     8041 gagatggtca caatcccgat gtactcaccc acgaagaaca gaccgaactt cataccggaa
     8101 tattcaatgt ggtaaccatc cgccagttcc tgctcggctt ccggctggtc aaacgggtga
     8161 cggtgacata ccgccacgcc cgcgatggca aaggtaataa aaccaaagaa ttgcgggata
     8221 acgttccaca catgcgcctg gctgttgacg atgtcggtca tgttgaatga accggcctgc
     8281 gccaccacgc ccatcaggga aagcccgagg aacacttcgt agctcagggt ctgcgcagaa
     8341 gcacgcatcg cacccagcaa cgagtatttg ttgttacttg accagcccgc aaacagcacc
     8401 gcgtaaaccg ccagacctgc catcatcagg aagaacaaaa tcccgatgtt caggtcggca
     8461 accacccaac ccggactgac tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca
     8521 atcatcggtg ccagggtaaa gatgacgcga tccgagaatt tcgggatcca gtcttcttta
     8581 aagaacattt tgatcatgtc cgcaaccagc tggagcgaac cgccccagcc aacacggtta
     8641 ggtccataac ggttctggaa cagacccagc aggcgacgtt cgccaaagct catgaatgcc
     8701 ccacaggtga caaccaccag caggatcacc accgctttga ggatggtcag caggatctca
     8761 atcagttccg gtgatatcca actcattgtt gtgcctcctt gagatcctca agatgcgcgc
     8821 cagccagcac cggagcaatg ccggacatac ccatcggcaa gcccacctgc cctgccgtca
     8881 gtccttcggc gatttcaacc ggcagcgtga ccgtgttgcc atcgtaacta aaggagacgc
     8941 gtgtacctgc gttcacaccc aacttcgcgg catccgctgg gttgagtttg atgtacggct
     9001 gcggcatacg gctctggaag accggagcac gctgtgacaa ttcatcgctg ccaaacaggt
     9061 ggtaatacgg cgcaatacgc catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg
     9121 tgaagtaatc cagaccattc tcgctggttt caaacagacg cacgcccgga tcgccaaagc
     9181 gcagtttgcc gcccacttcg tcctggaatt tgttccacgc ctgcggggag ttccagcccg
     9241 gtgcccaggc aaacggcact tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg
     9301 agaaggtgaa catggtgtca atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg
     9361 cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa
     9421 tacggaatgt cgcatccggc gcagcatctt tgatacctgc cagttccggg attttcgcca
     9481 caacagcgtc aatcacatgg tcgagctgcg tccagtccac ttcacggctc agcagggtgc
     9541 tgtgcagcga gtgcagccag cgccagcttt ccagcatgac agttttgctg tcgtaataag
     9601 ccggatcgta aacctggaag aaacgttggg cgcgaccttc attattaatt accgtaccgt
     9661 cgctttcagc aaagctggcg gcggaaagta ccagatgggc gttttccatg atcgctgtac
     9721 gctgatggtc aaccaccatc accagcggtg ctttagccag cgcagcattc acgcgggtag
     9781 cagaagcgtg acgatgcaga tcgttttcca acaccaccac cgcgtcggcg cgtccggttt
     9841 ccagttcggt taacgcttct tcaagcgaac cgccacccat aatgcccagc cccatgctgt
     9901 tgacggaacg ggcaatcatg gtgataccga cgtcagcgcc gcgacctttc agggctttcg
     9961 cgacgttagc cgccgcctga atcacctcta agctaccggc gttcgtcccg gagataatca
    10021 acggtttctt cgcacctgcc agtgcctgca cgatgacgtc gattttgctt tgcagctcag
    10081 gttcgatacc gtcaaccgct ggtgcagagt tatccagcgc atgggcgatg gcaaaaccta
    10141 aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc cgcgatatca tccagacggg
    10201 tgtcatcaac gttggtaaca aacagcggat gcttcgcacg ttgaccgatg ttaaggattg
    10261 ccgcaatctg ccagtcagcc actttctgtg ctgccgccat ttcgcgcgct ttacctttca
    10321 ccgcctgacg cactgccagc gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca
    10381 ccagtaccgc atcgtaagat tcgatttcgc gcagagccgg agtataaatg ccgccttcac
    10441 gcagcacttt cagcgccagt tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga
    10501 agttttcttc gcccaccagt tcacgcagtg caaagttgct ttccacgctg gcacgcggag
    10561 aaccaatacc gatcactttc ttcgactgac gcagaatatc tgccgcgccc tgcattgcct
    10621 gttcggcgtt gagggtaatg aaatcatcgc cacgacgctg tactggctga cgcggacgat
    10681 ccttcaggtt gacgtaaccg taaccgaaac gaccacggtc gcagaggaag tagtggttta
    10741 ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc gtaacgttca ccggggctga
    10801 tgttacagcc gatggaacat tgctggcaga tgctcggcgc aaactgcata tcccatttac
    10861 ggttgtaacg ctcggagtgc gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca
    10921 ggttaccgga aaattcgctt tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt
    10981 cgtgcgcgcc ataaacgccc agatctgtac cgtcagcgta atctttgtag taacgcacac
    11041 agcgatagca ggcgatgcag cggttcattt cgtgagagat gaatggcccc aaatcctgat
    11101 tacggtgggt acgtttggtg aaacggtagc gacggaagct gtgtccggtc atcacggtca
    11161 tatcctgaag atggcagtta ccgccttctt cacataccgg acagtcgtgc gggtggttgg
    11221 tcattaacca ctcgaccaca ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa
    11281 taaaggtgcc atcggaagcc ggtgtcatac aggacatcac caggcgacca cgcgtgtctt
    11341 ccgcgttttg gtattgcttc accgcacact ggcggcaagc accgacgctt cccagcgccg
    11401 gatgccagca aaagtaagga atatcaaggc ccagagacag acaagcttcc agcaggttgt
    11461 ccgctccgtt gacctcgtat tctttgccgt ctacatgaat tgtagccatt agcatgcttc
    11521 cagttttctc ggtcagggac cgagcgttaa tcgaaattcg gttaccagcg ctctttcagc
    11581 aggttcggct gaatcccatt aatcaaatgg gtattgctga acggctgttt gattcccgcc
    11641 tcaaattctt cgcggaaata tttgatggcg ctctgtaacg gctccactgc accaggtgcg
    11701 tgggcacaga aagttttacc cgggcctaag aatcgacaca gttgctcaag tgtttcgata
    11761 tcgcccggct ggccttcgcc acgctccagc gcacgcagaa ttttcacgct ccacggcaga
    11821 ccgtcgcggc acggcgtaca ccagccgcag gactcacggg cgaaaaactc ttccaggtta
    11881 cgcaccagcg acaccatgtt gatctcatgg tcaaccgcca tcgccagcgc cgtacccaga
    11941 cggctgcccg ctttaccgat actttcgaat tccatcggca gatcaaggtg cgcttcggtc
    12001 aggaagtcag tccccgcgcc gcctggctgc caggctttaa atttcagacc atcacgcata
    12061 ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc cgaacggcag ttcccacagt
    12121 cccggatttt tcacccgacc ggagaagccc atcagcttgg tgccagcatc tttacttttc
    12181 gagatgttct gataccactc cacgccgtta gcgaggatcg ccggaacgtt acacagggtt
    12241 tcgacgttgt tgacacaggt cggtttgccc catacgccgg aggttgccgg gaaggggggt
    12301 ttcgagcgtg ggttagcacg acgcccttcc agggagttga ttaacgctgt ttcttccccg
    12361 cagatgtagc gccctgcccc ggtatggacg aacagttcga agtcgaaacc tgttcccata
    12421 atgtttttgc caagcaagcc cgcttcagtg gcttcggcaa tggcgcggcg cagatttact
    12481 gccgcttcga tatattcgcc acgcaggaag atgtagccac ggtaagcttt cagcgcaaac
    12541 gcggagatga gcataccttc caccagcagg tgcggcagtt gctccatcaa caggcggtct
    12601 ttataggtgc ccggctccat ttcatcggcg ttacacagca ggtaacggat gttcatggat
    12661 tcgtctttcg gcatcaggct ccacttcagg ccagtcgaaa agcccgctcc gccgcgccct
    12721 ttcagaccag cgtcttttac ctgattaacg atttcgtccg gagacagccc ggtcagcgcc
    12781 ttacgcgccc cttcgtaacc gtttttgctg cggtattcgt ccagccacac tggctgtttg
    12841 tcatcgcgca gacgccaggt cagcggatgc gtttcgggag tacggataat gtttttcatt
    12901 tataccgctc cagcagttca gggatcgctt ccggggtcag atgcgcgtga gtgtcctcat
    12961 cgatcatcat gtttggccct ttatcacagt tccccaggca gcaagttggc agcagcgtaa
    13021 agcggccatc aaatgtcgtt tgccctggtt tgatgttcag ctttttctcg agcgccgcct
    13081 gaataccctg atacccgttg atatgacaga ccacgctgtc gcaataacgg atcacatggc
    13141 gaccaaccgg ctggcggaag atctgactgt agaacgttgc cacaccttcg acgtcgcttg
    13201 ccggaatacc cagcacatcg gcgatcgcgt ggatcgcacc atccggcacc cagccacgct
    13261 gcttctgaac gattttcagc gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca
    13321 tctcgtgctc aatcgcttca cgctctgccg cactcagctc aaaagcctcg gtttgtggtt
    13381 gttgattctc gtgcataatt agcggtccac atctgacata acaaaatcga tactgcccag
    13441 ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg
    13501 cgcataactc ggcgtgcgga tacgagtgcg ataactcatg gtgctaccgt cgctggtcag
    13561 gtagtaactg ttgatcccct tggtcgcctc aatcatctgg aaagattcat tggcaggcat
    13621 caccggcccc cacgacactt gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt
    13681 gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct ttgaacgggc cttccggcat
    13741 gttgttgagg cactgctcaa gaatgcgcag actctggcgt agctcttcca ctttcagcat
    13801 tacgcgggtg tagcagtcag aaacaccacc accaaccggg atttcaaagt cgaagttttc
    13861 atagccagaa taaggacgcg ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc
    13921 cgcgccagtg gtgccccact ccagcgcctc tttcgcgcca taggcggcaa cgccctggga
    13981 acgacctttc agaatggtat tttgcagcgc cgctttctcg taagacgcca gacgtttcgg
    14041 catccagtcg aggaactcac gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac
    14101 gccgccaata cggaaccacg ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc
    14161 gtaaattttc tgacgatcgg taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg
    14221 aataaaggtc gagatgtaca gcaggtggct gttgatacgg aacagttcgg agagcataac
    14281 gcgaatgacg ttaacgcgat ccggcacggt gatcccggcc agtttctcta ccgccagcac
    14341 gtaaggcatt tcgttaacgc agccgccgag gtattcaata cggtcggtat acggaatgta
    14401 gctgtgccag gactggcgct cgcccatttt ctccgcacca cggtggtggt agccgatgtc
    14461 tggtacgcag tcgacaatct cttcgccgtc gagttgtaaa acgatacgga aagccccgtg
    14521 cgccgacggg tggttcggac cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg
    14581 cttcatcccc cactcttccg gtttgaaggt cagggcttcc atctccagat cctgtttggc
    14641 tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg
    14701 gtgacctttc caggtttgcg gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt
    14761 aatgccaaac agatcccagg tttcacgctc ataccagtta gcgttcggga acagtttggt
    14821 gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt
    14881 acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg
    14941 gtgtgtgcgc agacgttcgt ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg
    15001 cagtttcttt aagaaatcgc caacttccag taattgttca cgcttgatcc acacaacggg
    15061 aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc
    15121 gccaattacc ggatcatcaa gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa
    15181 gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc
    15241 gcgaaataga tttaggaata agcgaagaaa aatccctttg ccgacaggcg caaattaaat
    15301 ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc
    15361 tgattgcata ttggcgcgat aaacgccctg atcgccaacc acccaagaga gcggacgacg
    15421 ttctttgccg atagattcct gcaacagcat cagtgcctgc atgtacgctt caggacgcgg
    15481 cgggcagccc gggatataca catcaaccgg gatgaattta tcgacgccct gcacaacgga
    15541 ataaatatcg tacataccac cagagttggc acaggcaccc attgagataa cccattttgg
    15601 ttccagcatc tggtcataca gacgctgaat aaccggtgcc attttggtaa agcaggttcc
    15661 tgcaaccacc atcaggtcag cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg
    15721 cgccacgtca tgcaccgcgg taaacgaagt caccatctca acgtaacagc aggaaagacc
    15781 gaagttatac ggccaaattg agtttttacg accccagtta accatgtcat tgagcttgcc
    15841 cataaacacg tttttgttaa cttcttgctc cagagggtcg gttacgatct cctgcttttg
    15901 cagggggtaa cggtcgttct caccgttggg atctatgcgg gtgagcgtat aatccatctt
    15961 aatgcctcgc ggttagcgtt gacgattagc gatactgttc gtttccgggt tcatacgctc
    16021 gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata cgcaccagat aaaccagacc
    16081 tgccagtaac acaaaaataa aaattgcagc ttccacaaag cctacccagc cgctttcgcg
    16141 gatagaggtt gaccatgcga acagatacag cgcttcaacg tcgaagataa cgaagaacat
    16201 ggccaccaga taaaacttgg cggacaggcg taagcgggcg gagccgaccg agtcgatacc
    16261 ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccgcc
    16321 taccagcatt aggcaacaca ggccaatggc aacgataaga aagatagcga atgcccagtg
    16381 atgagcgatg acttcagtgg ttgttgacat actcattgct tactcatcaa aagtagcgcc
    16441 agattcactg ctcttcacgg cagatggacg ccacatcgat tcatggggag gaataaaaaa
    16501 aaccttacaa tcactgtaga aattctttta tacagctaat tgatgtggtc ttttactcct
    16561 ttctataacc ttttgtcaac tttaacaaaa gtttcttcac attagtttac ataatatcaa
    16621 caccattagc atttaatgcc ctttcacccc agatccttga cgactccagg ataattagat
    16681 gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa
    16741 tttgtgtcga ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc
    16801 attccataac aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg
    16861 attttttcag gcgattattt gtgcgttcgg gacgtgaatc tctggtgatt gaaaaatgaa
    16921 cagttttgta cgttctgcac tatgcgagga aggattttta ctaaaaaaaa gccgctgggg
    16981 tttaaaacac ccccagcggc tcgtttttta cactattgtc tcaggaatta tctatcgtcc
    17041 gtcgattact cgatatccct ttcaatcaac aatgaatcat ccccttccgg agcagacatt
    17101 gggctgtatt gccacggatt atggtagctc tccatcgcct gatagatcac ctgggccagc
    17161 tcattattac tggacgggtc atagcacagc aaatattcgg tatcaggtag cggcggtaag
    17221 ccatctactc cactcagcac acgcaggtcc gggctcatca tctcaaccgg ccttgccgtc
    17281 acgccaagac cggctttcac tgccgcacga acggccggaa gcgtcgaggc gacataagcc
    17341 agtcgccatg gaatatctgc tttattaagc gttgccagca ccatatcgcg aaacggactt
    17401 ggatcatcca gcaatacaag agggatcggc tcgccttttt gcaatacgta ttccgctgcg
    17461 cagtaccagt gtgttggcga ggtacgcagg ttcaatgcct taaacgctga aggacgatgg
    17521 gtggttacca tcaaatccac ttcctgggat tccagcattt ctgccatata ggcattacgt
    17581 ttaacgcgga catccagcgc taatttcgga taaaccgaac tcacgcgatt taacaggaaa
    17641 ggtaagatcg tatcggcaga ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta
    17701 ctgaacatta atgatgagca ggcctcatca ttaaaacgca ggattttcct ggcgtaacca
    17761 agaagttgaa tgccatgttc agttaacagt ttgttgcgac cgtgacgagc gaacagttct
    17821 ttcccaacga gttgttccag acgctgcatt tgctgactta cggcggactg agtacgacac
    17881 acagcggcag ctgcggcagc aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc
    17941 agatcgaggt cgaggttaat tatcggacga tttgcactta tcatatatta tcacttactg
    18001 gcggctcata ctgagctggt taatgctgtg cacacacaaa caagcaattc catttgtaat
    18061 gtgcctccct ggcagtttca tccggaaccc ggacgaaagt aaaaatgcat atgagttgca
    18121 ctaaaaaagc gactcacatt gttccgttat aatgcctgaa gtagatcaca gaatatatct
    18181 tcagggatcg catatctatt aagttactca ctcttttcta tttatgacat gcgcgtgttt
    18241 gtataaatgt aaatgtgagt ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga
    18301 acacgtacaa acagcaatgg tgaggcggca tcaagagcag ggatccgtca tttatgcgag
    18361 cattttaccc caaaaacctt ttatttataa gggccattgc gaatcatctg atgcaaagtt
    18421 atgttatgtt aggcaaagta atcttctatt attaataagc acatcaaaac ctttttgaat
    18481 attaaataat aattaattag catcatcctc attcattaat tccgcttaac aatagtttca
    18541 caccttgccc ccattacgac acctcggaaa tcatcacgct gttaaactct ccgtaatgcg
    18601 gaaatatcat catcgcgttt cattagtgaa ttcttaaatg aggcattttc acacaattat
    18661 cttacagata aaaaaaccag acttacaatt aagaatcaga ataattcact atataacatt
    18721 gcatgtaaag catatacacc tcattatttt gtcattatta agttattaac agcacaatcg
    18781 agccttcccc tctggcaaaa tcttattctg cagaccttca aaacaccgtc ctgggggagt
    18841 acattgttct aagctgactt ccacggcagg gagtggcgat aacagcaaaa aaggtcaaga
    18901 ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg tctgttatga catccgtggt
    18961 ccggtgctga aagaagcaaa acgcctggaa gaagaaggta acaaggtact gaaactgaac
    19021 atcggcaacc cagccccgtt cggttttgac gcgccagatg aaatcctcgt tgacgtgata
    19081 cgcaacctgc ctaccgctca agggtattgc gattccaaag gtctttactc cgcgcgtaaa
    19141 gccatcatgc agcactacca ggctcgtggc atgcgtgatg ttaccgtgga agatatttac
    19201 atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc aggcattgct gaacagcggg
    19261 gacgaaatgt tggttcctgc accagattac ccactatgga ccgcggcggt ttcgctttcc
    19321 agcggtaaag cggtgcatta tctttgcgat gaatcctctg actggttccc ggacctcgat
    19381 gatattcgag ctaaaattac gcctcgtacg cgtgggatcg ttattatcaa cccaaataac
    19441 ccaaccggcg cggtatattc caaagagctt ttaatggaga ttgtggagat tgcacgtcag
    19501 cataatctca ttatcttcgc cgatgaaatt tatgacaaaa ttctttacga cgacgctgag
    19561 catcactcaa ttgcgccgct ggcacctgac ctgctgacca ttacctttaa cggactgtcg
    19621 aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg tgttgaacgg gccgaaaaaa
    19681 cacgccaaag gctacatcga aggtctggaa atgctggctt caatgcgcct gtgtgctaac
    19741 gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt atcagagcat cagtgaattt
    19801 attacccctg gcggtcgtct ttatgagcag cgtaaccgcg cgtgggaact gatcaacgat
    19861 attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt atatgttccc gaaaatcgac
    19921 gccaaacgct ttaacattca cgacgatcag aaaatggtgc tggatttcct gttgcaggaa
    19981 aaagttctgt tggtgcaagg gacggcattc aactggccgt ggccggatca cttccgcatt
    20041 gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga gcaagttcgc gcgtttcctt
    20101 tctggttatc atcagctgta atcttaattt cactgccgga gattgcatcc ggcagcgtta
    20161 tcccgccaca atgacctgat gatgtcatca tacgtaaggt cactatgaaa cagagccatt
    20221 tttttgccca tctctcccgc ctgaaactca ttaaccgctg gccgctaatg cgcaacgtgc
    20281 tgacggaaaa tgtgtccgaa cacagtttgc aggtagcgat ggtcgcccat gcgctggcag
    20341 ctatcaaaaa tcgaaaattt ggcggtaatg tcaacgccga acgtatcgct ttactggcga
    20401 tgtaccacga tgcctcagaa gtgctcaccg gcgatctccc tactccggtg aaatacttca
    20461 attcgcaaat cgctcaggag tacaaggcta ttgaaaaaat cgctcagcaa aaactggtcg
    20521 atatggttcc ggaagagctg cgggatatct ttgcgccgtt aattgacgag catgcatata
    20581 gcgatgaaga aaaatcgctg gtgaaacagg cagatgcact gtgtgcatat ctgaaatgtc
    20641 tggaagaact cgcggccgga aataatgaat tcttgctggc aaaaacgcga ctggaagcga
    20701 cgcttgaagc gcgtcgcagc caggagatgg actacttcat ggaagtattt gttcccagct
    20761 tccatctttc gctcgatgag attagccagg attcaccgct gtaagcagcc ggagtctgcg
    20821 tcgcatcagg caataagcgc cggatgcgac atcaggctct tgtcaaaacg gaaacagcat
    20881 cgggatcatc accacacaaa ccgccatcac gataatggtg aacggtaccc ccaacttcac
    20941 aaagtcactg aagctgtaat ttcccggacc taaaaccagt gtgttaacag gtgaagaaac
    21001 cggtgtcata aaggcggcgg atgctgccat cgccacgacc atcgcgaatg gataaggcga
    21061 cacccccatc gttttggcag cagccagcgc aatcggagcc atcaacaccg ccgtcgcggt
    21121 attagagata aatagcccaa taaccgccga caagacaaac aaacacccca gcatcatatg
    21181 tggcccgtaa ccaccgccaa tgtccatcag ccctttcacc gccagcgcga cacctcccgt
    21241 tttctgtaat gccacagcaa acggcatcat cccaacgatc aaaataatgc tcggccagtg
    21301 aatggattta taggcgcttt cagcatctat acagcggaat ttccccatca gcaggcaggc
    21361 gatgatagcg gcaacaggat taggaatttc atctgtcagc attaacgcca ccattagcac
    21421 cagacagaaa atggcatggg gtgcctggct gtgcgcgggc gatgcttcac tcacctcttc
    21481 cggtaagttc agcgctacga agtcgcggcc ctgtttggcc agcataccga tcagtttcca
    21541 gttacccaca accaggatga tatcgcccag cagcagaggc tcatccgcca gcgaaccttc
    21601 cagcgccacg ccattgcgct ttagccccac cacattcagt ccgtagcggg tacgaaaacc
    21661 aatttcgcgc accgatttac caatcagttc tgactcagga attaatgaaa tctctgccat
    21721 gcccacatca agggcctggt cagaaaaata ctcgccgcgc agtaccatcg gctccagcaa
    21781 ttgctcacta caaaattgcc ggagatcgac atcagccgca gacatatcaa taagcaaaac
    21841 gtcacgcgcg cgaaattcag aaaccccatt aacgttcacg ataacgcgac gaaaacgccg
    21901 ccagcgttca acaccgatga cgttagcgcc ataacgctca cgtaatttga gatcatccag
    21961 ccgttgacca atcattggcg atccggggcg aatagccaga cgtcgcgcac gcccggtcag
    22021 tcgatattca cggataagat cgcgaaaggt tcgacgcgtc cagccttcgc gctgcggggt
    22081 ctgggtatcc cctttcagca tgaaacgcat cactaacata tacaaaatac ccagcaccag
    22141 cacgaccagg ccaacaggtg ttacgctaaa gaaactgaag ccgtgatagc cttcacgcag
    22201 caattcactg ttgactacca ggttcggcgg cgtcgccacc agcgtcatca tgccgctaat
    22261 cagcccggca aaacttaacg gcatcatcag acgtgacggc gaagtttgca tacgcatggc
    22321 aacgcttaac accacgggaa taaagatagc gacaacgccg gttgaactca taaacgcgcc
    22381 aagccccgcg acggtcagca tcaacaaaac caacattttg atttcactgt tgcccgccac
    22441 tttgaccagc catgttccca ttacggtggc aacaccggta cggaccaaac catcgccaat
    22501 aataaacaag gcggcaatca ggacaacgtt aggatcagaa aagccggaaa atacttcggg
    22561 gaccgtcagc gttccgctta atgcaaacgc gacaataaca aacaaagcga ccgcatccat
    22621 acgcactctg cccgtcgcaa acaagacgat ggcaacggcc agtaatgaaa gaacccaaat
    22681 caattcaccg ttcacaactt atccttgtta attgaggggg atgacttgat tctgccataa
    22741 aaaagccccg acgagacggg gctaaatcat gatcaggtgt ttcactgaat aataacatcg
    22801 ccatttagct gtttggtcac agtaatttgc tccagactgt ggaggaccaa atcgacctca
    22861 ttcaggcgcg gggtatctgc cggagcgtta accgcaatga catggcaacc cgccgccagg
    22921 ccagaaagca cgccagcggg agcatcttcc gccaccacac actcctgcgg cgcaagcccc
    22981 agcagctgcg cacctaacag atacgcatca ggttctggtt ttccgcgctt cactcgctca
    23041 gcggttacaa acacctctgg tgcgggaagc ccagctattt tatggcgcgc tcgcgctacc
    23101 ggcatggagc cagaagtcac aatggcccac ggaatacctg ctttattcaa atgattgagt
    23161 aaggcgattg cccccggaag cgcagtaata ccttcggttt ccgtggcctc gatgtgctcc
    23221 agacgcgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaaa
    23281 gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga
    23341 cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca
    23401 tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcgt tgataatttg
    23461 attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaca cattaagcat
    23521 gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccatgagtcc cagcgtcaaa
    23581 atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc
    23641 agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg
    23701 caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga tgatagtgcg
    23761 gcaggtttct tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta
    23821 accacgctca ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat
    23881 agtcatcatc ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt
    23941 tgactcctgt atcactctac tacggtgaaa aaaaaaagaa ggctg
//
LOCUS       DAJTMK010000051        23362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTMK010000051 DAJTMK010000000
VERSION     DAJTMK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23362
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.052"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(75..416)
                     /locus_tag="OO135_001708"
     CDS             complement(75..416)
                     /locus_tag="OO135_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR5397597.1"
                     /translation="MKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNLFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSITDCSDRCSDYINPEHKKT
                     IKALQDAGYLK"
     gene            complement(388..4170)
                     /locus_tag="OO135_001709"
                     /pseudo
     CDS             complement(388..4170)
                     /locus_tag="OO135_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414580.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(4600..7011)
                     /locus_tag="OO135_001710"
                     /pseudo
     CDS             complement(4600..7011)
                     /locus_tag="OO135_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(7008..7694)
                     /locus_tag="OO135_001711"
     CDS             complement(7008..7694)
                     /locus_tag="OO135_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5397598.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7665..8288
                     /gene="tesA"
                     /locus_tag="OO135_001712"
     CDS             7665..8288
                     /gene="tesA"
                     /locus_tag="OO135_001712"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR5397599.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            8278..9087
                     /gene="ybbO"
                     /locus_tag="OO135_001713"
     CDS             8278..9087
                     /gene="ybbO"
                     /locus_tag="OO135_001713"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5397600.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLYSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            9148..10002
                     /gene="cnoX"
                     /locus_tag="OO135_001714"
     CDS             9148..10002
                     /gene="cnoX"
                     /locus_tag="OO135_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5397601.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(10065..10844)
                     /gene="fetB"
                     /locus_tag="OO135_001715"
     CDS             complement(10065..10844)
                     /gene="fetB"
                     /locus_tag="OO135_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5397602.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVRAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(10831..11508)
                     /gene="fetA"
                     /locus_tag="OO135_001716"
     CDS             complement(10831..11508)
                     /gene="fetA"
                     /locus_tag="OO135_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5397603.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            11654..12571
                     /locus_tag="OO135_001717"
     CDS             11654..12571
                     /locus_tag="OO135_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5397604.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12568..13026
                     /locus_tag="OO135_001718"
     CDS             12568..13026
                     /locus_tag="OO135_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5397605.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(13027..13434)
                     /gene="cueR"
                     /locus_tag="OO135_001719"
     CDS             complement(13027..13434)
                     /gene="cueR"
                     /locus_tag="OO135_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5397606.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(13559..14851)
                     /locus_tag="OO135_001720"
     CDS             complement(13559..14851)
                     /locus_tag="OO135_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5397607.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            complement(14854..15786)
                     /gene="glsA"
                     /locus_tag="OO135_001721"
     CDS             complement(14854..15786)
                     /gene="glsA"
                     /locus_tag="OO135_001721"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5397608.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPSVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            16048..18552
                     /gene="copA"
                     /locus_tag="OO135_001722"
     CDS             16048..18552
                     /gene="copA"
                     /locus_tag="OO135_001722"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5397609.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPVPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(18760..19101)
                     /locus_tag="OO135_001723"
     CDS             complement(18760..19101)
                     /locus_tag="OO135_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5397610.1"
                     /translation="MKQATRKPTTPGDILIYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTIVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            19239..20033
                     /locus_tag="OO135_001724"
     CDS             19239..20033
                     /locus_tag="OO135_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5397611.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            20237..20716
                     /gene="ybaK"
                     /locus_tag="OO135_001725"
     CDS             20237..20716
                     /gene="ybaK"
                     /locus_tag="OO135_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5397612.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(20753..22405)
                     /gene="ushA"
                     /locus_tag="OO135_001726"
     CDS             complement(20753..22405)
                     /gene="ushA"
                     /locus_tag="OO135_001726"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5397613.1"
                     /translation="MKLLQRRVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            22623..>23362
                     /gene="fsr"
                     /locus_tag="OO135_001727"
     CDS             22623..>23362
                     /gene="fsr"
                     /locus_tag="OO135_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5397614.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASI"
BASE COUNT         5301 a         6567 c         6053 g         5441 t
ORIGIN      
        1 aataaagata agtaattacg cataaaacaa caatgattat aatagcaaaa ataaatatta
       61 tcatctttga tagattactt gagatagcca gcatcttgta aagcctttat cgttttttta
      121 tgctctggat taatataatc actacatcta tctgagcaat ctgttattga tggacatgtc
      181 aacccatggt catttacagc caagtctttt ttattatctt caatcatttc agaatgggat
      241 agctttactt ttctgccgta cataccgaac agatttaacc cgtaatctct aatctctttt
      301 tcataaccca gccctttcgc cgatcggcta acaccagcgt catttaattt agacactcga
      361 caaaatgcca tacaatggaa aaattgatca gtcccaccta aatttaatct cttcataagt
      421 atcagataat atatcccatg ctctttgatg gtttagttga tcttttcttc ttattagcgc
      481 tacatctgcg ggacttaacc ctaatggatc aataccattc accggattca gcggatacgc
      541 atacagactc catcccccct ccagccctat cgggtcctga gtgatatacc gcccctgcaa
      601 cggatcgtag taccggttcc ggttgtagta cagccccgac tccttatcat actgctgccc
      661 cggcagccgg tacggctggt gcaggtgatg cgggttctcc tcattaagct ggttgcccca
      721 ttcatcatat tcctcgctcc acgccgtatt gccgtcttca ctgataagcg ccagcggcag
      781 gccccggtgg tcacagtgat aaagatgtat ttttcgctca ggcaaataaa ccggctctat
      841 ctgtgccgcc agccgctcca ccgtcagccc gcactgcgca agccactggc ggctttcact
      901 gctcacgcag tttgcccgga tttccccctc cagcctgtcc agcagcccca ccagttcagc
      961 cgggaacacc acaccgtgac cgtcttcgct cccttcctgc tgtaacttct ccgccaggct
     1021 gcggcactgc gctttctccc gttcaccgtt gtctgtttcg atgcggatga gcggcgcgaa
     1081 gctccccggc tggtataccg tctggatacg cgtggtgtcg gtctgtaccg tcgtcagccg
     1141 gtcgccgtcc catccgtacc acgtcacctc cggtttacgc gacagcgaca tccaccccgt
     1201 caggtcacgc tcgcgacgcc acacccgttt ccccgtccgg cggctcagcg ggtcgtagag
     1261 gtagcggctc tcgaccagtg gctcgccatg ctgtatccgc gtgtggaaca ccaggcggtg
     1321 ctggctgtcg tagtggtagt ggtgggtccg ctcgtcgtcc gtccgtatca cacccgccgg
     1381 gatgcggtcc gtcttctccg tcagtctgcc gtattcatca tagtggtaaa catagtgcgc
     1441 atcctctgcg atgcggttat ccggccacgc cgtgagcgtg ctgtccgggt gtagctccgg
     1501 gtccggcagc cggtttcccg ccgggtcggt cgcatacggg atgcggatgt ccaggtccgg
     1561 tgcgagggtg cgcacgctct ccagcctgcc cgtggcgctg tagccgtatt cccacgtctg
     1621 tcgcggaccg ctgatgcgca ccaggtcgcc gttgtcattc cacccgtagt cacggtcata
     1681 caccaggctg ttcaggtgct ggctctgtaa ctggcctgcg ggggtgtatg tgctggtcag
     1741 ttcatatgcg gcattactgc ctgccatgct gccgaagctg cgcaccgtct cacggtgcag
     1801 cctgtcccgc gtatactcga ccagcggcgt cccgcccagc ttcatgcccg ccaggtaacc
     1861 gctgccgtag gtcagccact ccaccggcgg caggctgtcc ggcgtgacgc ggtttgccag
     1921 cccctgttcg ttgtaggcgt gtttcgtctc atgctgccac agcagttccc ccgtctccgg
     1981 gttctccacc gtctgccgtt cgcccgtcag gcggccctta tcgtcgtagc cgtagtggac
     2041 agcgacacgg tggccttcgc tcaggtggct gatttctctc agccagccgt ggccgtcata
     2101 ctgccactgc tctgccgggt cgccgttcac cgtccggtgc gtgatgcggt ccgacgcatc
     2161 gtagtgccag agggtgacaa gcccctcatc ctcactctgc gtgagttttc cggtcaggtc
     2221 ataatgataa cgttgcgtcc gcccgtcaaa gccgccctgc tgtaccagcc ggtccagcgc
     2281 atcgtagctg aagtcgctgt gactgccgtt ctcgttagtc agactgatga cacgtccggc
     2341 agcatcgtac tccatactgc gcgtcagccc gccctgcgtg gtgctgaccg cctttcccca
     2401 cgcatcgtac tgtgtctcgc tccggttgcc gtccggggtg ataacggcag tcaggtcgcc
     2461 tgcggcgttg tactcatact gcgtttcacg gccctgtgcg tctttcaccg aggttaaccg
     2521 gccacggttg tcatagtggc ggtaaaggct gataccttcc tcgcggtgaa ccgccgtcat
     2581 ctgccgggtg ctgcccgtcg catccgttgt cgtcgccggt aactcactgt gcgcgtcatc
     2641 gtagcggtag cgtactgtct ccccgctgcg cgatgtctcc gataccagcc tgcccggttc
     2701 atcatattcc cggcggctct ccagcccgtc cgggtacacc accgccgtca gctggttccc
     2761 gtcgttatag taaaatttcg tctcccgccc gtccggtgtg gtgatgtccg tgatatcgcc
     2821 ggacaccaca ttcagaccgt actctgtccg ccgtcccgcc gcgtccgtct gcgccgtgag
     2881 ccttcctgcc gcgtcatacc cgctgcgcgt gacgctgccg tccgccagtt cttttttcac
     2941 cacccgtttc agcccggccc cgccttctgt atgcagcacc tcacgccggt tcaggctgtc
     3001 ggtgacggtg atgcggtcct gctcataaag atagcggtag cttaaccctg ccgggttcag
     3061 ttgctccacc acccgccccg tatcgtcgta gcggtagcgc atctccggcc ttcccgcgta
     3121 acggtgcgcc accatccggc ccaggtgctg cgcgtcatac gtgaaagcgc gcacctgcgt
     3181 attgctgcgg tcatataccg ccagcagttc accggcttcc gtatacgtgt accgcgccag
     3241 tggcgcaccg ggcaggctct ccgggtatgc cgggtcgtgc atcagccaca ccgccgaaag
     3301 gcggataccc ctgtcggggc cgtactctgt gctctccggc agtgaatcag gaatgtccgg
     3361 tcgttttgct ccggcgacga ccgcctgctg ccgggcctct tccgcccgct gcgcctgtgt
     3421 ggtcagcacc agacggaact cccgcccggc accgtccgtc acgccggtga tttccccggc
     3481 caggtcgcct gcggcctcac gccggtacgt cagcgtccgc ccgaagcggt ccgccatccc
     3541 ggtaagcacc cggtacggcg gcagcggcgc tggcagtacg tcctcagcac ccggcacccg
     3601 ctcagatcac cccagtatcc accacggccc ctgtgcgctg ttggtcgcca ggtaaagatg
     3661 cgggcttaac cggatatccg gcggcagcga cgcccacagc cgtgccagcg tgtggccgtc
     3721 cggctgcgct gccttaccgc cacgcaccag ccacattgac tcactgcggc tgtacacagc
     3781 ctcccccggc agcagcgact caaagtgaat gctccgcccg ccgttgtcgt tgagtaccag
     3841 tgcatcatcg cgtatctgta agcggatatc agaaggcgct ttccagccgg ggccaaaaat
     3901 tcccactggc gcaggcgtct tcgtccggta gctgctgtag gtgcgggaga gaatgaacgg
     3961 cagcgggtcg ggcagcgcaa ggtccgtctc gccgggctgt acttttgcac cgagcagcgg
     4021 gttcaccggg ttgcccgagg tcatcccgcc cggacacacc gagcacgcca cgccagttgg
     4081 cgcgccaatt cttactcctg ccgaaccctg gacaatggga ccgccatact gagtcatatc
     4141 tccctgacgc gccgctggtt ttccgctcat ataaagtgcc cttttagtta ttgttcatat
     4201 ccctgtgtta gcaacaaaaa aaataaaatg acacacaaga ccagctaatg tatttatata
     4261 tttttcacat aaagttattt gttctcaatc aacaataact gttattgcag gtcactttca
     4321 atattttatt aaactaaaat ttttagcaca gatgaattca tcatcaacaa attcactata
     4381 tggataatat ccattcaacg catatgattt atgattttat tagtctgagt tatttttgta
     4441 gggctatatt taaaattatt aaactccacc gattcagatt aatatcagca atagaaagat
     4501 aggaaatcac caattcaata acatcatcaa tgaaacatat tgtagaatta atttaaacaa
     4561 ggcgttccat tcaaccagaa cgccccttat ttactctcat caccccgcaa actgcctgaa
     4621 cagcgcctta cccttaacca gtcgcgcacc cagccagccg ccgaaaagcg acagcagcag
     4681 cgcaccactg cacggcagaa caatccacaa tcgccagtct ggctcccacg ggaaatcaaa
     4741 cactttcgcc tgcaacacta ccagtgccgt ttccgcacca attgcggcca ccaggccgga
     4801 aacaaaccca agcatggcga actcacacca caatgtcgtg cgcagcagtt ttttccccgc
     4861 accgagtgtg cgccatacca ccagctcctg atgacgctgg cgcattccca cctgcacctg
     4921 tgccagcaac agcaacatac cgcaggcggt gaccagcacc accatcacct ccagcgcccg
     4981 acttacctgc tccagcacct gaccgacctg ttttaaaatc gcgccaatat ctaacaggct
     5041 aatggtcggg aactggcggt tgagttgcgt caacatgccg ttgccattct cccagcggaa
     5101 actggtaagc cagctctgcg gttgcccgtc taatgcccct tccgggaaaa taaaatagaa
     5161 attaggccgc agactttccc ggtccacttt gcgcaggctg gtcactttag cgcggaactc
     5221 ctgggtatcg cccataaaag tcacggtatc accgagggca acgtttaagc gtttcgccag
     5281 cccctcttcc atcgacactt catcggcttt tggcggccag ttaccggcga caatcggatt
     5341 atgatcgggc cgcgtatttt gctaggtaag attgagttcg cgattaagcg cctcatcttc
     5401 attaccttct gtcggcttat cgttaatcgc cgtcagccgc gcccgcacta ccggataaaa
     5461 tgattccggg actatatgat gttccgcgag gaacactttt agcggtgcta cctgttctgt
     5521 ggcgatgtta attaaaaagt agttcgggct ttctggcggt agctgctgcc agcggtcgag
     5581 cagatcgcca cgcaatacca gcaacagcgc cagcagcata aaggagagcg aaaatgccga
     5641 aagctggctt aacgttgacc acggctgacg taacaggcgg ctaaccgcca ggcgcagagg
     5701 tagcgatttc agcgtcatgc ggcgaagtac attcagctgc atccagccca gcacaccgca
     5761 cagcaaagcc agtactaccg cgcccgccag caccgcccaa agcagcatgc tgccacccat
     5821 taatccggcg agcagcagca caaccaccat actgacaatc ggcagataaa acttcagcgg
     5881 ccagacgttc gctaccacat cattacgtaa tacgcgtaaa ggctgtgttg ccagcaacaa
     5941 gcggtatggt cgtagcccca ccagcagcga gatgaccgtc atggtgccaa gcgcccacag
     6001 ccacggccag aggctggctg gcggtagtgc agcaggcaga acgggcttga gcagcaccat
     6061 caacacgttt tcgaacaaca ggcctatggc cccaccggta acggctgaaa gcgtcagcac
     6121 catcaaccac tgaccgacga ttagcttacg cagttgcgct cgccctgccc ccagcgtttt
     6181 gaggatcgcc accagatcgt agcgactgcg acagtaatga ttcatcgcta ccgacaccgc
     6241 tgccactgcc agcagcaagg tcagaagcgc cgaaagcagc aggaactgtt gcgagcgttc
     6301 catcgatcgc cccagcgcgc cttcgtcctg ttccagaccg taccagcgtt gttcgggttt
     6361 aagctgaggt aacaaccatt tctcatagcc gtcgagctgg ttctcgttgc cgccgaattt
     6421 ataacgccag gtgacccgac tccccggctg cacggctccg gttttatcga catccgccag
     6481 attcatcatc agacgcggag ccatctggaa ggggttaaaa ccggaatccg gttcctgaat
     6541 cacttctccg gcaatccgca aggtggcatc gcccacgtca atggtgtcgc ccgttttcag
     6601 gtttagcagt gccatcaggc gtggggccag caatacgctg cccgcctgcg gtttcaggcc
     6661 agggggatta gtttgcagat cgccatacat cgggtagata tcatccaccg ctttgacgtt
     6721 cgccagctgc ggtgtgtcgc ctgcaaaggt cattgtggcg aaagtcagct gcttgccgac
     6781 tttcaggccg cgcttttgcg cttcctccag ccacgcttgc ggcacttcgc gtgaactgcg
     6841 caacgcccga tcgcccgcca taaactcacg gctttgctgg cttaagccct tctccatgcg
     6901 atcgctgata ttgcccagcg ccagcacgca ggccaccgcc aggcttagcg ccagccagac
     6961 aattaatagc gacggcgaac gccattcgcg ccagaaccaa cgtgcaatca tgcttcctcc
     7021 tgcaactgcc cgttcaccag ccgtaagcag cggtcgcagc gtgccgccag ttgcaggtcg
     7081 tgggtcacca taatcaacgt ggtgccatgt tcacggttga gggaaaacag caggtcggca
     7141 attttatcgc ccgtctggcg gtcaaggttg ccggtgggtt cgtcggcaaa cagcacatca
     7201 ggtcgaccat taaaggctcg tgccagcgcc actcgttgct gttcaccgcc ggaaagctgt
     7261 gccggaagat gatccagacg tttacccagc cctaactgtt cgagcaacgc tttcgccccg
     7321 ttacgacttt ccgcgctact ctcaccgcgc agcagagccg gaagctcgac gttttccagc
     7381 gcgttaaggg taggaattaa cataaatgac tgaaaaacaa agccgacgtg cttcgcgcgc
     7441 aactttgccc gcgcttcttc gtccatatta tgtagcggtt gtcccaccag actcacttcg
     7501 ccactgctgc cgtcatcaag cccggcgagg atcgccagca aggttgactt acccgatccc
     7561 gactcgccca ccagtgcgat ggtctcgcca cgtttgacaa ccagctcaac tccggtgagg
     7621 atggagagtt catgctcccc ctgaccgacg gacttcttaa gatgatgaac ttcaacaatg
     7681 ttttccgctg gcatttgccc ttcctgttcc tagtcctgtt aaccttccgt gccgccgcag
     7741 cggacacgtt attgattctg ggtgatagcc tgagcgccgg gtatcgaatg tctgccagcg
     7801 cggcctggcc tgccttgttg aatgataagt ggcagagtaa aacgtcggta gttaatgcca
     7861 gcatcagcgg cgacacctcg caacaaggac tggcgcgcct tccggctctg ctgaaacagc
     7921 atcagccgcg ctgggtgctg gttgaactgg gcggcaatga cggtttgcgt ggttttcagc
     7981 cacagcaaac cgagcaaacg ctgcgccaga ttttgcagga tgtcaaagcc gccaacgctg
     8041 aaccattgtt aatgcaaata cgtctgcctg caaactatgg tcgccgttat aatgaagcct
     8101 ttagcgccat ttaccccaaa ctcgccaaag agtttgatgt tccgctgctg ccctttttta
     8161 tggaagaggt ctacctcaag ccacaatgga tgcaggatga cggtattcat cccaaccgcg
     8221 acgcccagcc gtttattgcc gactggatgg cgaagcagtt gcagccttta gtaaatcatg
     8281 actcataaag caacggagat cctgacaggt aaagttatgc aaaaatcggt cttaattacc
     8341 ggatgttcca gtggaattgg cctggaaagc gcgctcgaat taaaacgcca gggttttcat
     8401 gtgctggcag gttgccgaaa accggatgat gttgagcgta tgaacaacat gggatttacc
     8461 ggcgtgttga tcgatctgta ttcaccagaa agtgttgatc gcgcagcaga cgaggtgatc
     8521 gccctgaccg ataattgtct gtatgggatc tttaacaatg ccggattcgg catgtatggc
     8581 cccctttcca ccatcagccg tgcgcagatg gaacagcagt tttccgccaa ctttttcggc
     8641 gcacaccagc tcaccatgcg cctgttaccc gcgatgttac cgcacggtga agggcgtatt
     8701 gtgatgacat catcggtgat gggattaatc tccacgccgg gtcgtggcgc ttacgcggcc
     8761 agtaaatatg cgctggaggc gtggtcagac gcgctgcgca tggagctacg ccacagcgga
     8821 attaaagtca gcctgatcga acccggtccc attcgtactc gcttcaccga caacgtcaac
     8881 cagacgcaaa gtgataaacc agtcgaaaat cccggcatcg ccgcccgctt tacgttggga
     8941 ccggaagcgg tggtcgacaa agtgcgccat gcttttatta gcgagaagcc gaagatgcgc
     9001 tatccggtaa cgctggtgac ctgggcagta atggtgctca agcgcctgct gcctgggcgc
     9061 gtgatggaca aaatattgca ggggtgagtt gaagcgcgcg cttaagcccc catgtcaaag
     9121 aaaatatcga caacagagag cgactccatg tccgtagaaa atattgtcaa cattaacgaa
     9181 tctaacctgc aacaggttct tgaacagtcg atgaccactc cggtgctgtt ctatttttgg
     9241 tctgaacgta gccagcactg tttgcagtta accccgatcc tggaaagcct cgcggcgcag
     9301 tacaacgggc aatttattct ggcgaagctg gactgcgacg cggagcagat gattgccgcc
     9361 cagtttggtc tgcgcgcgat tccgaccgtg tatctgttcc agaacgggca accggtagat
     9421 ggcttccagg ggccgcaacc ggaagaggcg atccgcgccc tgctggataa agtgctgccg
     9481 cgcgaagaag agctgaaagc gcagcaggcg atgcaactga tgcaggaagg caattacacc
     9541 gatgccctgc cattgctgaa agacgcctgg cagttgtcga atcagaacgg ggagatcggc
     9601 ctgctgctgg cagaaacgct gattgcgctg aaccgttctg aagatgcgga agcggtgctg
     9661 aaaaccattc cgttgcagga tcaggatacc cgctaccagg ggctggtggc gcaaatcgaa
     9721 ctgctgaagc aggcggctga tacgccggaa attcaacagt tgcaacagca ggtggcggag
     9781 aatccagaag atgccgcact ggcgacgcaa ctggcgctgc aactgcatca ggttgggcgc
     9841 aatgaagagg cgctggagtt gctgttcggg catctgcgta aagatctcac cgccgcagac
     9901 ggtcagacgc gtaaaacgtt ccaggagatc ctcgctgcgc tgggtacggg tgatgcactg
     9961 gcgtcgaagt atcgccgcca gctgtatgca ttgttgtatt gatattccgt tgttcggcac
    10021 atgaaccaca acgccggatg cgatgctgcg catccggcat cgcatcattt cttcttcaat
    10081 tgcgtcacca ccaactggtg gcgcgaatta taaaacttac gataggttaa atagcaggca
    10141 ataatggtcg acaagctggc ggttgagagc agcataaagg tcaccataat ctgatattta
    10201 atcgccctca ccggatcaat cccggcaaat atcagcccgg acatcattcc tggtaaactc
    10261 actaagccaa ccgtttttgc tgaatcgacc gtcggaatta aagccgcgcg aatactgtcg
    10321 cgaatcaata tcgctgaagc ctgcttcggc gtcgcgccaa gactcagttt ctcctgaatc
    10381 tgttgctgtt cgctaatgac ccgttgccct aaattgttgt aacacaaccc caccgctacc
    10441 atggcgttac cggcaatcat cccggcgata gggatcacct gcatcgggat aaattcaatc
    10501 gaccctgaga gaatcagcac cgccagggta attcccgccc cgaccgtaat ggcaataaac
    10561 gatgagataa aagctttagc aatatattta ctgcgttttt gcgcgttcca cgccgcatta
    10621 aagcagataa ataacaccat caataatgtc aggctggcat catccacgct gaaaatatac
    10681 ttcagcacat agccgacaat aatcagctga attatcgcgc gcccgacgct ccagaaaata
    10741 tctttctcca gcgccagttt ttctttatgg ctaattaaga ttgccaccac caccagcatt
    10801 aatgccagtg ccaatgattc gttagtaata ttatgcgagt tcatagcgtg cttcctgcat
    10861 ttctccggca tgcggttgca gtgtaatcac tttatccgca tgattaattt cgtctttatc
    10921 gtgtgtcacc cacagcacgg caatattttg ctcgcgcaca taacgatgga tcatctcatt
    10981 aacgttatgt ttattacttt catccagcgc actggtgatt tcatccagca ataaaacctt
    11041 cggcataaat tgcaggttac gaatcagtga gatgcgttgt ttttcaccac cagatagctc
    11101 ggcgatattt ttcgtcaaaa tgctgtccgg caaggcgaag cgttcgagaa aatcgagaaa
    11161 aatggctggg tcaggctgcc ggttacggat ctgccaggga aagatcagat tatcgtatac
    11221 cgtatcgcca aacagcgtcg gtgtctgggc gcagtaagag acttgttggc ggtagatttc
    11281 tggctttagt gtgctgacat cctcaccttc aaacagtaac gttccgctgg ttgggctgat
    11341 caatgaagca actattttta gcagcgtact tttgccacaa ccagaaggac cggtaattaa
    11401 cttaaattcg ccagcacgca gcgaaaaatt gatgttatta agaatcttcg catcacccgc
    11461 cagatatcct acgttttgta gctgaagcaa aggactattt tcctgcatcg ctgttccctt
    11521 tttctgattt ttactaaaaa cagtttatcc ttcgcaggaa taagggggaa ctctctttca
    11581 gtaatcaggt ataatttgtt taatcaagca gcaattttgc tgattatgga cggtacaaca
    11641 ggaggttttt ccgatgctta tctttatccc gattctcatt tttgtcgcgc tggtcattgt
    11701 cggcgcgggc gtcaaaatcg tgccgcaggg ctatcaatgg acggtagaac gttttggtcg
    11761 ctataccaaa acgttacagc cggggctcag tctggtggtg ccgtttatgg atcgcattgg
    11821 tcgcaagatc aatatgatgg agcaagtgct cgatatccct tcccaggaag ttatctcgaa
    11881 agataacgcc aacgttacca tcgacgcagt ctgttttatt caggtgattg acgcgccacg
    11941 cgctgcctat gaagtcagca atctggagct ggcgatcatc aacctgacca tgactaacat
    12001 ccgtaccgtg ttgggttcaa tggaacttga cgaaatgctc tctcagcgcg acagcatcaa
    12061 ctcacgcctg ctgcgtattg tcgatgaagc caccaacccg tgggggatta aagtcacccg
    12121 tattgaaatt cgcgacgtgc gcccaccggc agagcttatc tcttcaatga acgcgcagat
    12181 gaaagcggaa cgtaccaaac gcgcttacat tcttgaagcg gaagggatcc gtcaggcgga
    12241 aatcctcaaa gccgaaggtg aaaaacagtc gcaaatcctg aaagcggaag gcgaacgtca
    12301 gtcggcgttt ttacaggctg aagcgcgtga acgttccgcc gaagcagaag cccgcgccac
    12361 caaaatggtg tctgaagcta tcgcctccgg tgatattcag gcggtgaact acttcgtagc
    12421 gcagaaatac accgaagcgt tacagcagat cggttcctcc agtaacagca aagtagtgat
    12481 gatgccatta gaggccagca gcctgatggg gtcgattgcc gggattgccg aactggtgaa
    12541 agacagcgcc aacaaacgga ctcagccatg atggagttaa tggtcgttca tccacatatt
    12601 ttctggctca gtctcggcgg tttgctgctg gcagccgaga tgctgggcgg aaatggttat
    12661 ttgttgtgga gcggcgtggc agcggtgatt accggcctgg tggtctggct ggtgccgctg
    12721 ggttgggagt ggcaaggggt aatgtttgcc atcctgacgc tgctcgccgc ctggctgtgg
    12781 tggaaatggt tgtcgcggcg ggtgcgcgaa caaaagcaca gcgatagtca tttaaaccag
    12841 cgcgggcagc agctgattgg ccgacgtttt gtgctggaat ctccgctggt caacgggcgc
    12901 ggtcatatgc gcgtcggtga cagttcatgg cctgtcagcg ccagcgagga tctcggtgca
    12961 ggtacgcatg ttgaagtcat tgcgatagaa gggataaccc tacatatccg agccgtctcg
    13021 tcttaatcac cctgcccgat gatgacagca gccggagaga ttttcgataa tcgggcagtc
    13081 ggcgctgtca tcgccagggc aggcattcgc cagtgccagc agctggtcgc gcatggattg
    13141 cagctcctca atgtgtcgtt cgatctccgc caccttctcc agcgtgcgcc gtttgacgtc
    13201 ggcgctgtgc cgctgcgggt cgttaaacag attcaccagc tcgccgctct cttccaggtt
    13261 aaagcccacc tgccgtgcct ggcgcagtaa ggtcagttcg ttgagatgct gctgcgtgta
    13321 ggtgcgataa ccgttttcgc tgcgcatcgg cggcgtcacc agccccttct cttcatagaa
    13381 gcgaatggct ttgctagtca ggccggtaat ttttgctaca tcgctgatgt tcatcgttcg
    13441 cgcaacgcct cctgtgcttt gttaaagggt ttcaacagat attcaagcac gtttttttgc
    13501 cagtgcgaat atcgaccgcc accacaataa tacaggaagt actgcgcaca gagagatgtt
    13561 atacggtttt attgcgcttc atgaccattg ccacaataag gctgagtaag agtgagccaa
    13621 taatccatat caacgcacgg ctaccctggg accacagact gtagataaag ccaacgatca
    13681 ccaacaacat caccaacgta ccaacgataa gaatcggcaa cgaggcgtga atatcatgac
    13741 gcaggcggat cgccaccaca aacaccgcca ggtagcaaat caaaaaggtg gcgctggcaa
    13801 cgctggcgag tgagcctaaa ttcagtgccg ccgtcatcag cataatcaac accacgacaa
    13861 tgatgttgcc ccaggtactc tgccgccaca gggatttatt cattagcttc ggcagttcgc
    13921 gttcgctgcc catgttgtcc atgatgttaa acacggcgaa caggttcgcg ttaatggctg
    13981 aagccgtcgc tagtaaagcg ccgatgacga cgatcacata acccacatgt ccgagcagcg
    14041 gagaagcagc ctgcgctacg gcggtatcgg catatttttc taactctaat gccgatacat
    14101 cgctaagcaa aaccagtgcc agcgagatat aaagcaacgt ggtaacgcca atcgccacca
    14161 gaaacgcccg tggcataatg acctgcggat ctttcacttt atccgccgcg ttcgccatca
    14221 tgccaaagcc cgcataggca aggaaagtta tcccaataca ggagaagaac gcaccggagc
    14281 tggggggcgc agagacggaa atatgcgctg gttgcagcga ccagacaccg gcaataatca
    14341 gcaataacag gatcatcatt ttaatgccga cgaggatcac ttccagccgc cctaccgcat
    14401 ggttgcttaa ggagttgaaa agcgtcatca ccgcaatgct ccccaacgcg tagagcaaaa
    14461 taaggtgctc ctcctggctg ccttcatgca aaaactgcac ggcataagcg ccaaaagcat
    14521 gggcgaccat ggcgatgctc accgccagcg tcaacaggta cagtaacgag agcgccagcg
    14581 aaaagacgcc gttgcctaat ccgcgacgaa agaagtcgat aatgccgcca ttgctgggat
    14641 agctcgcccc cagacgcgca taggcataac cggaaaacat cgccacaata ccgccaaaag
    14701 caaaggcgac ccaggtcgag gcttccatta gcaacgcagc ctgccccagc agcgcgaaga
    14761 tccccgcccc caccattgcc ccaataccga tggaaacgac attccataga ccgaggggtt
    14821 tgttaccgtt attaccttcc gtgttcatca tgatcagccc ttaaacacgt tatagccgag
    14881 ttgcttagcg accgatgcca ccattttttg accgcgaaca ctgttacctt cttcgtccag
    14941 cggtggtgag aacgcggcaa ttcccatcac gctagggacg accgccagaa taccgccacc
    15001 tacaccgctt ttgcccggta aaccaacgcg atacgcccag tcaccggagc gaccatacag
    15061 cccttccatc atcatttcgg ccagaatgta cggcacgttg tcggcctgaa gaacgcgttt
    15121 atgcgtcagc ggattcagac cacccgccgc cagcgtcgcg ccaagcgttg ccagttcaac
    15181 agtattgatg agcgtggagc actgacgggt gtacacgtca caggcttcca ttgcatcaca
    15241 atagagatat ccggcggagt acagcagcca ggctatggcc cggttatgga agttggttgt
    15301 ttgttccgac tggttgactt cgtcagagag cgctacctgc tcaccagcca gttgctgttg
    15361 gatatgtaaa attcgctgcc agcgttgttc aatattttca gcgttaatca ggctggtggt
    15421 ggcaatagcg ccagcattta ccagtggcga aagcggtttg ccgccatgca actctaaggc
    15481 gataactgag ttaaagggca atccggtcgg gtcagcgcca attttgtcct gtaccgcctg
    15541 cgggccgaca tcttctaacg caagggctaa cgtacagact ttcgagatgg attccagtgc
    15601 aaagcggtaa tcactgtcac ccgcactata gacgttgcca tcgcaggtca cgatagccac
    15661 tgccgccagt tgacctggta cattcgccag aaagggaatg taatcggcat tttgtccgcc
    15721 gttaagtgag tgaaattggg tgtaagcctg atccactgcc tgctgtaatt tgtttgcatc
    15781 taacatcttt tgttaactcc tttttataga tgcgggaggt aattcctcac cccggtgccg
    15841 attttcaggc atcctgattt aacttagcac cagcaactta actacaggaa aacaaagaaa
    15901 taaatgtcta atcctgatgc aaatcgagcc gattttttaa tctttacgga cttttaccca
    15961 cctggtttat taatttcttg accttcccct tgctggaagg tttaaccttt atcacagcca
    16021 gtcaaaactg tcttaaagga gtgttttatg tcacaaacta tcgacctgac cctggacggc
    16081 ctgtcctgcg gtcactgcgt taaacgcgtg aaagaaagtc tcgaacagcg cccggatgtg
    16141 gaacaagcgg atgtgtctat cactgaagcg cacgttaccg ggactgccag tgcagaacag
    16201 ctgatcgaaa ccatcaaaca agcgggttat gacgcatctg taagccaccc aaaggctaaa
    16261 ccgctggcgg agtcatcaat cccgtcggaa gcactgacag cggtttctga ggcgcttccg
    16321 gcagcgaccg ccgatgacga tgacagccag cagttgctgc tgagcggcat gagctgcgcc
    16381 agctgtgtca cccgcgtaca aaatgcgctg caaagcgtac cgggcgtcac tcaggcacgg
    16441 gtaaacctgg cggagcgtac tgcgctggtg atgggcagtg cctccccaca agatttagtg
    16501 caggcggtgg aaaaagcggg ctacggcgcg gaagcgattg aagatgacgc gaaacgccgc
    16561 gagcgccagc aggaaaccgc cgtcgctacg atgaagcgct tccgctggca ggcgattgtc
    16621 gcactggcgg tgggtattcc ggtaatggtc tgggggatga tcggcgataa catgatggtc
    16681 accgctgaca accgcagcct gtggttagtt atcggcctga taaccctggc ggtaatggtt
    16741 ttcgccggcg ggcattttta ccgcagcgcg tggaaaagcc tgctgaacgg tgcggcgacg
    16801 atggatacgc tggtggcgct gggtacgggt gtggcgtggc tctattcgat gagcgtcaac
    16861 ctgtggccgc agtggttccc gatggaagcg cggcatcttt actatgaagc cagtgcgatg
    16921 attatcggtc tgattaatct cggccatatg ctggaagcgc gcgcgcgtca gcgttcttct
    16981 aaggcgctgg agaaattact cgatttaacc ccaccgacgg cgcgagtggt caccgcagag
    17041 ggtgaaaaaa gcgtgccgct ggcggaagtt cagccaggga tgttactgcg cctgacgacc
    17101 ggcgatcgcg tgccggtaga tggcgagatt acccagggcg aagcatggct ggatgaagcg
    17161 atgctgacgg gcgaaccaat cccgcagcaa aaaggcgaag gcgaaagcgt ccatgccggg
    17221 acagtggtac aggacggtag cgtgctattt cgcgccagtg cggttggcag ccataccacg
    17281 ctgtcaagga ttattcgcat ggtgcgccag gcccagagca gcaagccaga aatcggtcag
    17341 ctggcggata aaatctcagc cgtatttgtg ccggtagtag tggtgattgc gctgatcagt
    17401 gcagcaatct ggtatttctt tggtccggta ccgcagattg tctataccct ggtgatagcc
    17461 accacggtgc tgattattgc ctgtccgtgt gcgctggggc tggcgacgcc aatgtcgatt
    17521 atttccggcg tcgggcgggc ggctgagttt ggcgtgctgg tgcgggacgc cgacgcgctg
    17581 caacgcgcca gtacactcga taccgtcgtg ttcgataaaa ccgggacgct gaccgaaggg
    17641 aagccgcagg ttgtcgcagt aaaaacattt gctgatgttg atgaagcgca agcattgcgt
    17701 ctggcggcgg cgctggagca aggttccagc catccgctgg cacgagcgat cctcgataaa
    17761 gcaggcgata tgcagctgcc gcaggtcaac ggtttccgca cattgcgcgg gctgggcgta
    17821 agcggtgaag ctgaaggtca tgcgttattg ctgggtaatc aggcgctgtt aaatgaccag
    17881 caagtcgata ctaaagcgat tgaagccgat atttcagctc aggcatcgca aggagcaacg
    17941 cctgtgctgc tggcgattga cgggaaagta gtagccctgc tggctgtacg cgatccgttg
    18001 cgtagtgata gcgtggcggc gctgcaacgc ctgcataaag cgggatatcg tctggtgatg
    18061 ttgaccgggg ataacccaac caccgccaat gcgatcgcca aagaagcagg gattgatgag
    18121 gtgatcgccg gggtgctgcc agacggtaaa gccgaagcga tcaaacgtct gcaaagtgaa
    18181 ggacgtcagg tggcaatggt gggcgacggc attaacgacg cgccagcgct ggctcaggcg
    18241 gatgtcggta ttgcgatggg tggcggcagt gatgttgcca ttgaaaccgc ggcgattacc
    18301 ctgatgcgcc atagcctgat gggtgttgca gatgcgctcg cgatttcccg cgcaacgctg
    18361 cgcaatatga agcagaacct gctcggtgcg tttatctaca acagcattgg tatcccggtc
    18421 gccgctggta ttttgtggcc gtttactgga acactgctta acccggtagt tgccggagcg
    18481 gcaatggcgc tctcgtcgat taccgtagtg agtaacgcca accggttgct gcggtttaaa
    18541 ccgaaggaat aagtacgtca ttgcggatgc gctgctttag ggcggcgcat ttggcatcga
    18601 cgcatgactg ccggatgcgg cgtaaacgcc ttatccggcc tacggttcag gcacaggttt
    18661 gtaggcctga taagacgggt aagcgtcgca tcaggcaatg gtgctcggat gcggcgtgaa
    18721 cgccttatcc ggcctacgag atgaagcgat gttttggcct tacgcgacct tttttgcacg
    18781 ctcttcacgg cgtgccaaat attcagccac tgtttcaatc cgtcccaatt cttcttgggt
    18841 gcgcatgttg ttttcaactt cccaaagatc aactgccgcc tggaggttta gccaaaaatc
    18901 tactatggta tcaaaaactt tcgccagacg gaatgccatc tcagtggtga gtttacgatt
    18961 attattgatc agtgtactga cgctattacg atgaacatgc agcaactctg ctaactcatt
    19021 gattttcaaa tcgagcggtt ccagatattc atagataaga atatctccgg gtgtcgtcgg
    19081 ttttcttgtt gcctgtttca tggtttttaa ctgttgcttc cctgtgaata gcgatgcaag
    19141 aacatactga atcatacaaa cacattaaac aagtccccaa ctccatttgc gccatccctc
    19201 accgcgcgct agcatgatat ttcacaaagg gaggtcgtat ggatctgttg taccgggtaa
    19261 aaacgctttg ggccgcgctg cgcggtaatc attacacctg gcctgccatc gatatcaccc
    19321 ttcccggcaa tcgccatttt catctgattg gcagtattca tatgggtagc cacgatatga
    19381 ctcccctgcc cacccgtttg ctcaaaaagc tcaaaaacgc cgatgcgctg atcgtcgagg
    19441 cggatgtttc caccagcgat acgccttttg ctaatttgcc tacttgcgag gcgctggaag
    19501 agcgcattaa cgaggagcaa ctccaaaatc tgcagcacat tagccaggag atgggcattt
    19561 ctccgtcgct cttttctact caaccgctgt ggcaaatcgc gatggttctt caggcgacgc
    19621 aggcacaaaa actggggctg cgggcagaat acggtatcga ttaccagtta ttgcaggcgg
    19681 cgaagcaaca acataaaccc gtgattgaac tggaaggggc tgaaaaccag attaccatgt
    19741 tgctccagct ccctgacaaa ggactggcgc tgctggacga tacgctgacc cactggcata
    19801 ccaacgcacg gttgctgcaa caaatgatga gctggtggct gaatgcacct ccgcaaaata
    19861 atgatataac gctgcccaat acgttcagtc agtcgctgta cgatgtgctg atgcatcagc
    19921 gaaatctcgc ctggcgggat aaattacgcg ccatgccgcc ggggcgatat gtggtcgcgg
    19981 tcggtgcact acacctgtat ggagaaggga atttgccgca aatgttgcgc taaaaaaatg
    20041 gccaatatcg ctattggccc gtcaaagagg aatttcattt ttttattatt atgccgtcac
    20101 tttaagcgac ggtgtaactc gattgtcgct caatctcacc atcctgccaa tactcttagg
    20161 caagatgact cttttttttg gaccgccatc aggcgtatgc ttgcttcgtt tttttgttta
    20221 aggcaggaaa attaccatga cacccgcagt taaattactc gaaaaaaaca agatttcgtt
    20281 tcaaatccat acctacgagc acgatccggc tgaaaccaat tttggcgatg aagtcgtcaa
    20341 aaaattaggt ttgaatccgg atcaagtcta caaaacgcta ctggtggcag tgaacggtga
    20401 tatgaaacat ctggcagtgg ccgttacgcc ggtcgcaggt caactggatc ttaaaaaagt
    20461 agcaaaagcg ctgggtgcca agaaagttga gatggccgat ccgatggccg cgcagcgttc
    20521 gacgggatac ctggttgggg ggattagccc actggggcag aaaaaacgtc tgccgacgat
    20581 tatcgacgcc cccgcacaag aatttgccac tatttatgtt tctggcggca agcgcggact
    20641 ggatatcgaa ctggcggcag gcgatctggc gaagatcctc gatgccaaat ttgctgatat
    20701 cgcccgccgc gattaagcat tgtgccggat gcaaatatcc ggcactttcg gattactgcc
    20761 agctcacctc acctttcggt tcataaacac tcacatccag cggcgagctt ttctggatat
    20821 acgctttcag cacttcggca tcaataaagc cggtattcac atagcccggt ttgttatcaa
    20881 ggcgcggata gccatcaccg ccggtggcat tgaagtttaa tgtcgccata cggtaagttt
    20941 tcgccggatc gaccggttcg cctttgattt tcaggtcgtt gagtttgccg tctttcgtca
    21001 caaagctaac gttggcaaat tgcgggtagg cacctgaatc tggcttcatt tgtgcgacgg
    21061 cggtcaggta atcaatcacc tctttaccgg tcatgtcggc atacaccacc acattgccga
    21121 atggctgcac tttcagtacg tttttatagc tgatatcgcc tgcttcgata gaatcacgaa
    21181 ttcctcctcc actcatcacc gcaaagtcgg caccagtgcg atccatttgg gctgccagaa
    21241 tcaaccgccc catattggtc tgtacaaaac gcactttgtc acgatcgcct tccagacgac
    21301 cattggtttc gcctattttc acttccaact gcgctttgcc tttgttctgg aacggcgata
    21361 acagcgagat catttgctgg ttttcagcga tttcaggagt gtaaagcacg cgctcgcttt
    21421 tcccgtcttc ccaggtcact ttcttcttca ggttcaccgg aatcagctgg tagttaacca
    21481 ttttcatttc gccattacga aactcaaaat cagcccgtcc cacgtatttg ccccactcat
    21541 gcgcctgcac aatccagatg ccgttttgtt gatctggttt gcatggcgta cccggcacgt
    21601 aatcgacctg ttttttgttt tctgccgcca tgcagaccgg agcttgcgag tgaccaccga
    21661 cgatcatcgc cagcgatccg gcaggcagcg cgcgtgccat ctccacatcg cccggtgcgt
    21721 tagagccgtg ctcaccatta tcgtaatgcc ccatatgggt cgccgcgata ataatgtctg
    21781 gcttttctgt ctgttgcagc tcctgaatca ccagcttcgc ttcatcggcg ggcttacgaa
    21841 attcgacatc agtgaagtat tccgggttac caatttttgc tgtgtcatcg gttgtcagcc
    21901 caatgacggc aattttcaga tcctgacgct taaacagcgc ccatggttta aacaggcgct
    21961 cgccggtact tttctggtag atattcgcgg aaagcaacgg gaacttggcc cacttttcct
    22021 gctggcgtaa tacggtgagc ggattatcaa attcatgatt accgatcgcc atcgcgtcat
    22081 agcccaccag attcataccg cgaaaatcag gttcggcatc ctgtaagtca gactcgggca
    22141 cgccagtgtt aatgtcgcca ccggaaagta gcagcacgct accgccttca gccgcaacct
    22201 ctttgcggat accatccacc agcgtttttt gcgccgccag accatattcg ccatattcat
    22261 tgcgccaaaa atgcccatga tgatcattgg tatgcagaac tgtaattttg taggttttat
    22321 cctgctcata cgccagagca gtttcactcg ccagtgtaaa tgtggttaac agcgctaacg
    22381 ccacgcgccg ctgcaataat ttcatacttc tctccctgac ctgatttcaa cgtgttgaaa
    22441 aatagtatga cgcaacatca tagacaagtc tgcgtccggt gaagataaat ctgcatccag
    22501 attagaaatt ctgtattcgg cgttgagact tccttcaggc gacgcataca ggtatgttag
    22561 tcgggtaata atcacaaata agatcattct ttacatcgca accctgaaaa agtgactttc
    22621 ctatggcaat gagtgaacaa acccagcctg tggcgggcgc ggctgcgtca acgaccaagg
    22681 cccgaacatc gtttggtatt ttaggtgcta tcagcctctc acatctgctg aacgacatga
    22741 tccaatcgct gattctggcg atttatcctc tgcttcagtc agaattttct ctgacattta
    22801 tgcagattgg catgataacc ctcaccttcc agctcgcctc ttcgctactg caaccagtgg
    22861 tcggctactg gaccgataaa tatccgatgc cgtggtcgtt gccaattggc atgtgcttta
    22921 ccttaagtgg tctggtgctg cttgcgctgg cgggcagttt tggcgcagtt ctgctggcgg
    22981 cggcgcttgt cggtaccggt tcatcggtct ttcatccgga atcttctcgc gtagcccgta
    23041 tggcttccgg cgggcggcat ggcctggcgc aatctatctt tcaggtcggc ggcaactttg
    23101 gcagttccct gggacccttg ctgacggcgg tgattatcgc gccttatggc aaaggcaacg
    23161 ttgcctggtt tgtgcttgcg gcactgctgg cgatcgtggt gttggcgcaa attagccgtt
    23221 ggtactcggc acagcaccga atgaataaag gaaaacccaa agcgacgata atcaatccac
    23281 tgccgcgcaa caaagtggta ctggcagtca gcattctgtt aatcctcatt ttctcgaaat
    23341 atttctatat ggcgagcatc ag
//
LOCUS       DAJTMK010000052        23101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTMK010000052 DAJTMK010000000
VERSION     DAJTMK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23101
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.053"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="OO135_001728"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="OO135_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5397615.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVITIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="OO135_001729"
     CDS             complement(1696..2010)
                     /locus_tag="OO135_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5397616.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2210..4168)
                     /gene="acs"
                     /locus_tag="OO135_001730"
                     /pseudo
     CDS             complement(2210..4168)
                     /gene="acs"
                     /locus_tag="OO135_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4561..5997
                     /gene="nrfA"
                     /locus_tag="OO135_001731"
     CDS             4561..5997
                     /gene="nrfA"
                     /locus_tag="OO135_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5397617.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWVGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6042..6608
                     /gene="nrfB"
                     /locus_tag="OO135_001732"
     CDS             6042..6608
                     /gene="nrfB"
                     /locus_tag="OO135_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5397618.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6605..7276
                     /gene="nrfC"
                     /locus_tag="OO135_001733"
     CDS             6605..7276
                     /gene="nrfC"
                     /locus_tag="OO135_001733"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5397619.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7273..8229
                     /gene="nrfD"
                     /locus_tag="OO135_001734"
     CDS             7273..8229
                     /gene="nrfD"
                     /locus_tag="OO135_001734"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5397620.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMLVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPAHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8249..9967
                     /locus_tag="OO135_001735"
     CDS             8249..9967
                     /locus_tag="OO135_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5397621.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9960..10343
                     /gene="nrfF"
                     /locus_tag="OO135_001736"
     CDS             9960..10343
                     /gene="nrfF"
                     /locus_tag="OO135_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5397622.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10340..10936
                     /gene="nrfG"
                     /locus_tag="OO135_001737"
     CDS             10340..10936
                     /gene="nrfG"
                     /locus_tag="OO135_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5397623.1"
                     /translation="MKEPKIPVKMLTALTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYTALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11278..12591
                     /gene="gltP"
                     /locus_tag="OO135_001738"
     CDS             11278..12591
                     /gene="gltP"
                     /locus_tag="OO135_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5397624.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLMHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12782..13470)
                     /locus_tag="OO135_001739"
                     /pseudo
     CDS             complement(12782..13470)
                     /locus_tag="OO135_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13564..15711)
                     /gene="fdhF"
                     /locus_tag="OO135_001740"
     CDS             complement(13564..15711)
                     /gene="fdhF"
                     /locus_tag="OO135_001740"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15292..15294),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5397625.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWIAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15909..17375)
                     /gene="mdtP"
                     /locus_tag="OO135_001741"
     CDS             complement(15909..17375)
                     /gene="mdtP"
                     /locus_tag="OO135_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5397626.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLQEEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17372..19423)
                     /gene="mdtO"
                     /locus_tag="OO135_001742"
     CDS             complement(17372..19423)
                     /gene="mdtO"
                     /locus_tag="OO135_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5397627.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSNWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19423..20453)
                     /gene="mdtN"
                     /locus_tag="OO135_001743"
                     /pseudo
     CDS             complement(19423..20453)
                     /gene="mdtN"
                     /locus_tag="OO135_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
     gene            complement(20472..20747)
                     /locus_tag="OO135_001744"
     CDS             complement(20472..20747)
                     /locus_tag="OO135_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397628.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20956..22941)
                     /gene="yjcS"
                     /locus_tag="OO135_001745"
     CDS             complement(20956..22941)
                     /gene="yjcS"
                     /locus_tag="OO135_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR5397629.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5212 a         6065 c         6296 g         5528 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcagcccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcacg ccgaggataa tcgtaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaatcaatg cctgatgcga cgctgtcgcg
     2161 tcttatcagg cctacaaacc gttaccgact cgcatccggc aattgtgggt tacgatggca
     2221 tcgcgatagc ctgcttcact tcaagcagct tctcgactac gccaggatcg gcaagcgtcg
     2281 aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt gcgcagaata cggcgcataa
     2341 ttttgccgga gcgggtttta ggcagggagt cggtccagtg cagcacgtct ggcgtcgcca
     2401 gcgggccaat ctctttacgc acctagttgc ggacttctgc gtacagttct ggtgacggtt
     2461 cctccccgtg attaagcgtg acgtaggcgt agatcgcctg acctttaata ttgtgcggaa
     2521 tacctactac ggcggcttcg gcaatcttcg gatgcgccac cagcgccgac tcaatctctg
     2581 ccgtccccag acggtgaccg gagacgttca gcacgtcgtc cacacgcccg gtgatccagt
     2641 aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa atacatattt ttgaaggtgg
     2701 agaagtaggt ctgttcaaaa cgttcgagat cgccaaacag cgtacgcgcc tgacccggcc
     2761 aggagtcggt gatcaccagg ctgccttcgg tagcgccttc cagcgggtta ccttcgttat
     2821 cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc cgaaccggct ttcagctcgg
     2881 tagcgccagg cagtggcgtg atcatgaaac cgccagtttc ggtctgccac caggtatcga
     2941 ccaccggaca tttctcgttg ccgatttttt tccagtacca ctcccacgct tccgggttaa
     3001 ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg gtcggtaccc tcgatcgctt
     3061 tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc agtatagaga atattgacct
     3121 gatgcttgtc caccacctgc gccatacggg caggcgtcgg ccagttgggt acgccttcaa
     3181 acatcagcgt ggtcgcaccg caggccagcg ggccgtacag caagtaactg tgtccggtca
     3241 cccagcccac atcggcggtg caccagtaga tatcacccgg atgataatca aagacatatt
     3301 taaaggtcag cgccgcgtac accagataac cgccggtagt atgcagcaca ccttttggct
     3361 taccggtaga accggaggtg tagagaataa acagcggatc ttcggcgttc atctcttccg
     3421 cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca ccacaggtcg cgcccttcct
     3481 gccagtcaat tttcccgcca gtacgcttca gtaccaccac atgctctacg ctggtgacgt
     3541 tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg aatactgcgc ccggcacgca
     3601 caccttcgtc ggaagtgatc accagtcgtg agttggaatc aataatgcgc ccggcaacgg
     3661 cttccggcga gaagccgccg aaaatcaccg aatgcaccgc gccaatgcgg gcgcaggcca
     3721 gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat cgccaccaca tcaccttttt
     3781 taatgcccag ctcgagcagg gtattggcga agcggcagac gtcgcggtgc agctatttat
     3841 agctgatatg tttgctctgg ctggcgtcgt cgccttccca gatgatggcg gtacgatcgc
     3901 cgttttcttg cagatggcgg tcaaggcagt ttgccgccag attcagcgtg ccgtcctcgt
     3961 accatttaat ggacacatta ccgggggcaa aggaggtgtt tttcaccttc tggtaaggtt
     4021 tgatccagtc aagaattttt ccctgttcgc cccagaaggt atcaggtacg ttaatagatt
     4081 gttgatacat tgcctcgtac tgctgagggt ttatcaggca acggtctgcg atgttggcag
     4141 gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc cttgtagaat gttaataata
     4201 tgtggcataa gcgttaaatg taggggtatt ggcagttttg tttagtattt gggcgacaga
     4261 tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa tttttgaagg tcagcaacaa
     4321 aagttgatta attctttgag gaacatgcag ttatgcatgc tgttgaaaaa gaggaagata
     4381 ctgactaact ctaaagtggt attttacatg cacttacaat tgattaaaga caacattcac
     4441 agtgtggtta tttgttacac ataggggcga gcaatgtcat gacagtgtag gtgcggttac
     4501 tgtcgtgaaa aacaataaaa accgccattg caacaatggc gcaattcgga tgaagcccct
     4561 atgacaagga taaaaataaa cgcacgccgt atcttcagct tattgattcc ttttttcttt
     4621 ttcacttctg ttcacgctga acaaacggca gctcccgcaa aacctgtaac tgtggaagcg
     4681 aagaatgaaa cctttgcccc gcagcatccc gatcaatatc tctcctggaa agccacctcg
     4741 gaacagtcag agcgtgttga cgccctggcg gaagatccac ggctggtgat cctgtgggtg
     4801 gggtatccct tctcgcgcga ttacaacaag ccgcgtggac atgcctttgc tgtgaccgat
     4861 gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg aagatggtcc gctaccgatg
     4921 gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga tccagaaaga cggcgaagat
     4981 ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa tcgtcaacaa cttaggttgt
     5041 gccgactgcc ataacaccgc ctcaccagag ttcgccaaag gcaaaccgga gttaaccctt
     5101 tcccgcccgt atgcggctcg cgcgatggaa gccattggta aaccttttga gaaagccgga
     5161 cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg tggagtatta cttcgacggc
     5221 aaaaacaaag cggttaaatt cccgtgggat gacggcatga aagtcgaaaa tatggagcag
     5281 tattacgaca aaattgcctt ctctgactgg actaactccc tgtcgaaaac gccaatgctg
     5341 aaagcgcagc acccggaata tgaaacctgg acagcgggca ttcacggtaa aaacaacgtg
     5401 acctgtatcg actgccatat gccaaaagtg cagaacgccg aaggcaaact ctacaccgac
     5461 cataaaattg gtaatccgtt tgataacttc gcccagactt gtgcgaactg ccatacccag
     5521 gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc agtcgattaa cgacctgaaa
     5581 atcaaggttg aagatcaact ggttcacgct cacttcgaag cgaaagcagc gctggatgca
     5641 ggcgcgacgg aagctgaaat gaagccaatt caggacgata tccgtcatgc ccagtggcgc
     5701 tgggatctgg cgatcgcttc ccacggcatt catatgcacg caccggaaga aggtttacgg
     5761 atgctcggta cggcgatgga taaagcggcg gatgcacgca ccaaactggc gcgcctgctg
     5821 gcgaccaaag gcatcaccca tgaaatccag atcccggata tctcaaccaa agagaaagcc
     5881 cagcaggcca ttggcctgaa catggaacaa atcaaggccg agaagcagga cttcatcaaa
     5941 acggtgatcc cgcagtggga agaacaggca cgtaaaaacg gtctgttaag ccaataaccc
     6001 cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa tatgagcgta ttacgttcgt
     6061 tattaactgc tggggtgctg gcgtcgggcc tgttgtggag cctgaacgga attaccgcta
     6121 cccctgcggc gcaggcatct ggtgatcgct acgaagttac ccagcagcgt aacccggatg
     6181 ccgcctgtct ggactgtcat aaaccagata ccgaaggtat gcatggcaaa catgcttccg
     6241 ttatcaaccc gaataacaaa ttgccggtca cctgcaccaa ctgtcacggc cagccatcac
     6301 cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa cgagccgatg tacaaggtcg
     6361 gagagcagaa cagcgtctgt atgtcctgtc atctgccgga acagttgcaa aaagcgttct
     6421 ggccgcacga tgtccacgta accaaagtgg cgtgcgccag ctgccattcc ctgcatccac
     6481 agcaagatac gatgcagacg ttaagtgaca aaggacggat taagatttgc gtcgattgcc
     6541 acagcgatca gcgcaccaat ccgaacttta acccggcgtc tgttccgttg cttaaggagc
     6601 agccatgacc tggtctcgtc gccagtttct caccggcgtc ggcgtgctgg cagccgtcag
     6661 cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc aatggggtgc gttacggcat
     6721 ggtgcatgac gagtcgttat gcatcggctg taccgcctgt atggatgctt gtcgagaagt
     6781 gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt cgtagcgagc cgcagggcga
     6841 atttcctgat gtgaaatatc gcttcttccg taagtcttgc cagcactgcg atcatgcgcc
     6901 gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat gccgctagcg ggattgtcga
     6961 tgttaatcct gatctctgcg tcggttgtca gtactgcatc gccgcctgtc cgtaccgcgt
     7021 gcgctttatc catccggtca cgaagacggc ggacaaatgc gatttctgcc gtaaaaccaa
     7081 tttgcaggcc ggtaagctgc ccgcgtgcgt tgaggcttgc ccgacgaagg cgctgacgtt
     7141 tggcaatctg gacgatccta acagtgagat ttcgcaactg ctgcgccaga agcctactta
     7201 ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac cgcgtaccgt ttaaatacgg
     7261 ggaggtgagc caatgacgca gacttccgca tttcattttg aatcgctggt gtgggactgg
     7321 ccgattgcca tctacctgtt tttgattggt atttctgccg gtctggtgac gctggccgtg
     7381 ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca gtacgttgct gcgcaccacg
     7441 ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga tcctcgtctt ccacctgaca
     7501 agaccgtgga ccttctggaa gctgatgttc cactacagtt ttacctcggt gatgtcgatg
     7561 ggggtgatgc tgtttcagct ttacatgctg gtgctggtgc tgtggctggc gaaaatcttt
     7621 gaacatgatt tgcttgccct gcaacaacgc tggttgccga agctggggat cgtgcaaaag
     7681 gttctgagcc tgctgacgcc cgctcatcgc ggactggaaa cgctgatgct ggtgttggcg
     7741 gtgctgctgg gggcttatac cggctttctg ctgtcggcgc tgaaatcgta tccgttcctc
     7801 aataacccga tcctgccggt gctgttcctc ttctccggca tctcgtccgg tgcggcggtg
     7861 gcgctgatcg ccatggcgat acgccaacgc agtaacccgc attccacgga agcgcacttt
     7921 gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct tcctgctggt ggcgtttttt
     7981 gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc tggtggcggc attaggtggc
     8041 ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc tggggctgat tgtgccaatg
     8101 ttgctcaaat cgtgggtcaa tcgcagttcc ggcattcctg ccgtgctggc ggcgtgtggg
     8161 gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc tctacgccgg acagttgacg
     8221 gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc cttcctgaag tcggttttct
     8281 ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg ttgacggcct ttgcgggagt
     8341 gcggttgcgc tggcctgcca tgatgcgact cacttgcatc ggcattctgg cgcagttcgc
     8401 gctcctgctg ctcgcctttg gcgtactgac gtattgtttt ctcatcagcg atttctcggt
     8461 catttatgtc gcccaacata gctacagcct gctgtcgtgg gaactcaaac tggcggcggt
     8521 gtggggcggt catgaaggtt cgctgctgct ttgggtgctg ctgctttccg cctggagcgc
     8581 gctgtttgcc tggcattatc ggcagcaaac cgatccgcta tttccgctga cgctagccgt
     8641 tttatctctc atgctcgccg cactgctact gtttgtggtg ctgtggtccg atcccttcgt
     8701 gcggatattt ccaccagcaa tcgaaggccg cgatctcaat ccgatgctgc aacatcccgg
     8761 tcttatcttt catccaccgc tgctttacct tggctatggc ggtttgatgg tagcggcgag
     8821 cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg gccagcgccc gaatttgctg
     8881 gcgctgggcg ttacctggct ggagtgcatt aacggcgggg atcatcctcg gttcctggtg
     8941 ggcctactgc gaactcggct ggggcggctg gtggttctgg gatccggtgg aaaatgcctc
     9001 tttattaccc tggctttctg ccactgcgct gctgcacagt ttatccctga cacgccagcg
     9061 ggggattttt cgccactggt cgctgttact ggcgatagtt actctgatgc tgtcgctgct
     9121 gggcacctta attgtccgtt ctggcattct ggtttcggtt catgcgttcg cgctggataa
     9181 cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt agccttgcgt ctctggctct
     9241 gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt ttttcggggt tatcgcggga
     9301 aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg ctactgatcg tgctggtggg
     9361 aacgctttat ccgatgattt acggtctgct gggctgggga cgcctctccg ttggcgcgcc
     9421 gtattttaac cgcgcgacgt taccgtttgg tctgttgatg ctggtggtga ttgtgctggc
     9481 gacgtttgtc tctggcaaac gcgtgcagct tccggcgctg gtagctcatg cgggcgtgct
     9541 gttatttgcc gctgggatcg tggtttccag cgtcagccgt caggagatca gcctgaattt
     9601 acagccgggt cagcaggtga cgctggcagg atacaccttc cgttttgagc gcctcgatct
     9661 gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg gcactgtttg accatcagca
     9721 acgcattggt gaactgatgc cggagcggcg tttttacgaa gcacgtcgtc agcaaatgat
     9781 ggaaccgtca attcgctgga acggcatcca tgactggtat gcggtcatgg gtgaaaaaac
     9841 cggagcggat cgttacgctt ttcgcttgta tgtacaaagc ggtgtgcgct ggatctgggg
     9901 gggaggattg ttgatgattg cgggcgcatt gttaagcgga tggcggggga ggaagcgcga
     9961 tgaataaagg acttctcacg ctactgctgt tatttacctg ttttgcgcgc gctcaggtcg
    10021 tagacacctg gcaattcgcc aatccgcaac aacagcaaca ggcgttaaat attgccagcc
    10081 agttacgttg tccgcagtgc cagaatcaaa atttactgga atccaacgcg ccggtggcag
    10141 tcagtatgcg ccatcaggtt tacagcatgg tggcggaggg gaagaacgaa gtcgaaatca
    10201 ttggctggat gaccgaacgc tacggagatt ttgttcgcta taacccaccg ttaacgggtc
    10261 agacgctggt gttgtgggcg ctgccagtgg tgttgttact gctgatggca ctgatcctct
    10321 ggcgagtgag ggcgaagcga tgaaagagcc caaaataccg gtgaaaatgc tgacagcact
    10381 cacgattttg atggtatttc tctgtgtcgg cagttatctg ttaagtccaa aatggcaggc
    10441 ggtacatgcg gaatatcagc gtcagcgcga tccgctacat cagtttgcca gccagcaaac
    10501 cccggaagcg cagcttcagg cattgcagga taaaatccgc gctaatccac aaaacagcga
    10561 acaatgggcg ttactgggcg agtactatct gtggcaaaac gattacagca attcgctgct
    10621 ggcgtaccgt caggcgttgc aactacgtgg tgagaacgct gaactgtata cggcgctggc
    10681 gacggtgctt tattaccagg ccagccagca tatgaccgcc cagactcgcg caatgatcga
    10741 caaagccctc gcgctggact ctaacgaaat caccgctctg atgctgctgg cttccgatgc
    10801 gtttatgcag gcgaattacg cgcaagccat cgaactatgg caaaaagtga tggatctcaa
    10861 ctcaccccgg atcaaccgga cacagctggt cgagtcgatt aatatggcga aattgttgca
    10921 gcggagatcg gattaatcat ctggtgatga agattatgtt attgagctgc ataaaaaata
    10981 atcgaatgaa catatgccaa aaataatcac taatcagtat tattgcagat taaacaaata
    11041 aaaatctttc cataacaaat ggttattcat taatcctgac ataaactcgc gttattatgc
    11101 attaatgcag cgaaaagctc tgttgttaaa gggttgcgca acataccgcg caaatgatac
    11161 tgatcataag cgttaaaaaa atctacaaac caacgcaaca caattcatgc cctggcagta
    11221 tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca ttgaggaagt tattctgatg
    11281 aaaaatataa aattcagcct ggcctggcag attctgtttg ctatggtgct gggcattctc
    11341 ctgggaagct acctgcacta ccatagcgac agccgcgact ggctggtcgt caatttgctc
    11401 tctccggcgg gtgatatctt catccatctg attaaaatga ttgttgtgcc gattgtgatc
    11461 tccacgctgg tggtgggtat cgcgggtgtg ggtgatgcca aacagctcgg gcgtattggc
    11521 gcgaaaacca ttatttactt cgaggtgatc accaccgtcg ccatcatttt ggggatcact
    11581 ctggcgaacg tcttccagcc cggtgccggg gtggatatgt cgcagctggc gaccgtcgat
    11641 atctcgaaat atcagagcac tacggaagcg gtacaaagca gttcccacgg cattatgggc
    11701 acgattttgt cgctggtgcc gacgaacatt gtggcgtcga tggcgaaagg cgaaatgctg
    11761 ccgatcatct tcttctcggt gctgtttggt ctggggcttt cctccctgcc cgcgacgcat
    11821 cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa ccatgtttaa agtgactcac
    11881 atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga ttgcggtgac ggtggctaac
    11941 tttggtttct cttctctgtg gccactggcg aaactggtgc tgctgatgca tttcgccatt
    12001 ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc tgtgcgggtt aagcgtctgg
    12061 atcctgattc gtattctgaa agatgagctg attctggcgt actccactgc cagctctgaa
    12121 agcgtactgc cgcgaattat tgagaagatg gaagcctacg gagcaccggc gtcgatcacc
    12181 agcttcgtgg tgccgaccgg ttactctttt aaccttgatg gttcgacgct gtatcaaagt
    12241 attgccgcta ttttcatcgc tcagctgtat ggcattgacc tgtccatctg gcaggaaatc
    12301 attctggtgc tgacgctgat ggtgacctcg aaagggattg ctggcgtgcc gggcgtgtcg
    12361 tttgtggtgt tgctggcaac gctgggtagc gtaggtattc cgctggaagg tctggcgttt
    12421 attgctggtg ttgaccgtat cctcgacatg gcgcgtactg cgctgaacgt ggtgggtaat
    12481 gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat ttgaccgcaa aaaggcgctg
    12541 gcttatgagc gtgaagtgct gggcaaattt gataaaactg cggatcaata attgaagatt
    12601 gccggggtta tccacccggc aatgtgtgga tgcctgatgc gacgctggtg cgtcttatca
    12661 ggcctacgcc agacagcgca atagcctgat ttagcgtgat tttgtaggtc ggataaggcg
    12721 tttacgccgc atccgacatc aacgcctgat gcgacgctta acgcgtctta tcaggcctac
    12781 cttaaccgtt ggttaatttt tcaaactctt cacacccggt atcaaaccct tccatacagc
    12841 tcaggttcaa ccagtgcagc gccttttgtt tgttcttctc gataaaacct tcttcaccgt
    12901 ttaagaacat cattcccgcc cagtactcgg aataaccggt tcgggaaatt gcagagctgc
    12961 gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa
    13021 tcaaaccaag cagcatttgg gcatcgacgg cagagtcgtt ctccagatct tccgaggctt
    13081 ttctaacagc gaaatcgcct ttggataatc cggtttaccc gcttgagtat ttaccagaat
    13141 atgcgccagc gttacttcac cttctttact gcccgcttgc gccgctttct ctgcaagaac
    13201 ttttgcttgt ggatagtcca gactgaccgg attggtgatt ttgatctgcg ccaacagcgc
    13261 gcaggcatca gcatcaccgc tgtcggtggc tttctgtgcc caatattcgg ctttgctcaa
    13321 atcgccggag ctaaaccagc tgtcggcaag aaaatattgt gcgcgtcggt ccccggcctc
    13381 tgctgccttt aaatactggc tgccaggctc ggagtcgttg gcgtgggcaa agaatgtcaa
    13441 aaacaacatc aatgcgataa tttttttcat tttgataata cgattgggta gggagtaacc
    13501 agtataaaga gttcgaatgg ataaaaaaac agcctccgaa aggaggctgt agaaaggact
    13561 gtattacgcc agtgccgctt cgcgcaggcg agttttcaac ttgttgtact cgtcaatcac
    13621 gtactgctcg gcggcgcgct gatcggcgat cggctcgacg cgcacggcgc agtatttgta
    13681 ttccggcgtt ttcgtaatcg ggcttaagtt ttcggtaacc agctcgttac aggcaccaat
    13741 ccaccactgg taggtcatgt aaatcgcccc tttgttcgga cgatcgctga cctgcgcacg
    13801 ggtgataatt ttgcctttac gcgagtgcac ccaaaccaat gcctcatctt caatacccag
    13861 acgtttggcg tcttcggtat tgatttgtgc gtagccaggt tcatcagcca gcgccgccag
    13921 tgccgcacag ttaccggtca tcgaacggca agagtagtga ccaacttcac gcaccgttga
    13981 cagtaccatc gggtactcgt cggtgagttt gtcgattggc gctatccagt cgcaggtgaa
    14041 gaactgcgcc agaccgttcg gggtatcaaa cttctcttta aacagataag aagtcccctg
    14101 atcggcatct gaagtatcgc ggcaaggcca ctgaatgaag ccctgttcgc ccattttctc
    14161 gtaagtcgca ccgtagaaat ccgggcacag atgacgcaac tcatcccaga tctcctgggt
    14221 gttgttgtag tgcatcggat aacccatacg ggtggcgatt tcactgatga tttgccagtc
    14281 cgttttcaga tcccatttcg gttcaactgc tttgaagaaa cgctggaagc cacggtcagc
    14341 cgcagtaaac acgccttcat gctcgcccca cgacgttgac ggcaaaatga catccgccgc
    14401 cgacgcggtt ttggtcataa agatgtcctg aacgatgacc agttccagat cttcaaaggc
    14461 tttacgtact gccgacagct ccgcatcagt ttgcagcgga tcttcgccca taatgtacgc
    14521 ggcacgcact tcgccatgcg ctgcgcggtg cggcagctcg ctgatgcgat agccggtatg
    14581 agcaggcagg ctttccacgc cccaggcttt ggcgaatttc tcgcggttag ccggatcttt
    14641 cacgtactgg tatcccggat acgtatccgg cagcgcgccc atatcgcagg caccctgcac
    14701 gttgttctga ccacgaaccg ggtttacacc cgcatgcggc ttaccgaggt taccggtcag
    14761 catcgcgagg ctggtcagag aacgcacggt ttccacgccc tggtagaact gggttacacc
    14821 catgccccac aggatggcgg cgcttttcgc ctgggcatac atccgtgccg cctgacgaat
    14881 ctcactggcg ctgacgccgg tgatatcttc aaccgactcc ggcgtgtagc cttcaacgat
    14941 tttacgatac tcttcaaagc cttctgtacg ggaagcgacg aacgctttgt cgtacagatt
    15001 ttcttcaata atgacatggc ccatcgcatt caacagcgcg atgttcgagc cgtttttcag
    15061 tgcaatgtgc atgtcagcaa tgcgcgcggt ttcaattttg cgcggatcgc agacgataat
    15121 tttcgccccg ttacgtttag cgttaattac gtgattcgcc acgattgggt gggaatccgc
    15181 cgggttgtac ccgaaaacga acactaaatc ggtattatca atttcgttaa tagcattgct
    15241 cattgcgcca ttaccgaccg attggtgcag acctgcaacc gatgggccgt gtcagacacg
    15301 agcgcagcag tcaacgttat tggtaccaat aacggcgcgc gcaaattttt gcattacata
    15361 gttggtttcg ttacccgtac cacgcgagga gccggtcgtc tggatggcat ccggaccgta
    15421 cttctctttg atggcgctca ggcgctcggc aacgtaattc agtgcctcat cccaggaaac
    15481 aggttcgagt ttgccgccac gctggcgacg gatcatgggg gttttcaggc gcggggtcag
    15541 gatctgggta tcgttaatga agtcccagcc ataataaccc ttcagacaca gggtaccctg
    15601 gttggttttc ccctgcgctg cctccgcccg gacgattttg ccgttatcga cgaccaggtt
    15661 gattttgcaa cctgatgcgc aataggggca aaccgtgacg acttttttca tcggtctcgc
    15721 tccagttaat caaatcacgc atacgcgctc tcgactacag tacgcatctt ttatgccaca
    15781 ttttatttgg ggtcattccc tgatattacg ggcactattt attcaaaact ctgacgaaaa
    15841 acaggctgtc gtcagttttg acgtgacgaa acgaaatacc gcgtgacagc catcacgcgg
    15901 cagacatttt attttttctc gacgacggga gccgcctgat acccgccgcc cagcgatttc
    15961 atcaactgaa tgctgtggat cacccggcgg ctgtccagca tcagtaatga catctcttcg
    16021 gcaagcactg gcaaccgggc ttcggtggcc tgtaagcggc tggttaagcc gcgctgatag
    16081 gcggcctcgg cagcgcgctg ggtgaagcgc gttgcttcca cgcgttcagc ctgcatttct
    16141 cgttcgtcgt tgagcgtttg cagacgcgtg ccgttgacgg caacgtcacg caccgcgttc
    16201 agtactgact ggttgtaacg ttcaatcatc atgttgctgg cggcgcgagt gccttcgaga
    16261 ttggcattca accgtccacc gtcaaacagc ggcaatttca gacccgggat gaagttgaac
    16321 tggcgactgg tttttttgaa taaggtatcc aggtggatgg agtccagacc gaaaaacgct
    16381 ttgatatcaa agctcgggta gaacagtgcc cgcgcggaat ccacctgatc taatgacgcc
    16441 tgaacatacc agcgcatggc ttgcagatcc gggcgtctgg cgagcaactc ataagagagt
    16501 gtcgccggaa tgccggtctg gactcgtggt aatgccaccg gtttgatctc cggcatatcg
    16561 ctggcccccg cgccaatcaa tgcacgcaga gattctcgcg tttcggtgat ttgccctttg
    16621 acggcagcaa tttgtttatc gaccgccaga atctgtgccc gcgcgccgtg gaaaggcact
    16681 tgtgcttcca gaccgtgcgc cactttactc tggtgcgctt tcaccgcgta atcaatcaca
    16741 tcgcgagttt gttctaacag atcgagcatc tgatagctgg cctgcatact gtaataaagc
    16801 tgcgctacgc ccgtggtcag cgatagctct actgctgcgg tttctgccag cgcggcatta
    16861 tgcgcgccaa tggcggcggc aaccgctgag cgatgcacac cccacaaatc aagatccagt
    16921 ccggcaaaca aacctactgt ggcttccgta tagtacggcc cgtccatacc cagtgagggc
    16981 gcatccatcg cataagggct taaaaagccg ttcgccgaga cgcgttggcg gttgagcatc
    17041 cctaacgctg cgacctgtaa ttgtgaaccg gcatctaaca aatcggcctg ggactgcgct
    17101 ttttcttcct gcagtttcgc ttcggcgagg gtgtgtgaac cacttagcgt ccgttggatc
    17161 agcgcatcca gctgcgggtc attgagttgt ttccaccact gcgcctgcgg ccagccggag
    17221 ctggcaagat gaatatcgtc ggccagtttg atttgttccg gtttgagctg ttgatgaggt
    17281 gcagaatcct tacgtaccag ggcacagccg gaaatcagcg tcgtgctgcc gagaatgctg
    17341 cacaacagca gacgtgaaag ttgacgattg atcattgcgt ggctccttgt gcctgttccg
    17401 tggcgggcgt taatgccggg gctgtccagt ctggcaagcg ggcgaaaagc tggcagacgt
    17461 gttgctcctg ttttaataag gtgggcagga tggcctgcga ggcgggtgtc tcttcaagcg
    17521 ttatttgagg agaacagctg agtgcggttg ccagaccggc agcgtatttt tccagggcgt
    17581 cggcaaactg acctgctctg tccggctgta gtgcgttatc cagcgcctgc gccgagttcc
    17641 aggtggcatc ccaggcgtga agaagatcgc ggccctgacg aataaccgtt tgcgaacgtt
    17701 caatcagtaa ggcgcgttct tcgctgtcca gttgacgctc cagcgccacg cgttggcaca
    17761 tctcttcaca ggcattaaac gccgcatgca gacctatacg aatttgcaga taagtgcgca
    17821 gagccgtgac ttcctgctgg cgtgggatcc gcattacttt acttagcata cccagcgcac
    17881 cggctagttt ttgcggcaga gtgcgcgctt cactttcagg ccagacaaag gtgtaaatca
    17941 ccgcggacac cacggtacca atgatgatac ccagggcgcg atcgcgaatt tccaccaggt
    18001 cgtacactgg gccaaaaacg ttttcgagcg tggcgagcgc gaaggtgacg accatctgtg
    18061 tgccgatata agaagagcgt tcagagctgg tggcaatcca tgcgcccaac aggaaaatcg
    18121 gtgccagcac aaacagcaat tcgacaatat tgtccagcca gggcatgacc agtagcgtga
    18181 ataacagcgc cagaatcgcg ccgcaaaagg ccccgccaaa acgcagcacc atcttctggt
    18241 acgacgaacc gacatttgga ttagcgacga tcacgcaggt cagcatacag gtgtgaatgc
    18301 cttcccagtc cacgccgctg taaaaggtgt aacagatcaa acaggcgagc aatgttttta
    18361 ccgcgtagcg tatatagtct ggattggtaa aagcatcggc gaccattgat ggcggtttgg
    18421 cggcgggcgt tggcggcgta ttcgggttca tctggcccag ttgtaacaac gtctggcaga
    18481 tattctccag gttacattcc cgtgccgcca ccgcttcact ttcactgatc cgccagttgc
    18541 tttgccagca ctgaccttca gcaacggcat gctgcagctt gttgatttct gaggctaatt
    18601 tttgccgaaa ttcaataatt gcctgagaat cagcaaaaga ggtgggatcg tagcgattca
    18661 gcgtcgagta aatgtaggtt accgttgcca cgcagctttg ccaccatgcg ctttgagttc
    18721 gccagttggc atcgtccgcg agacaaaaga cattcagttt ttgcagcgcc agcgcctctc
    18781 tttcaatccg cgtttcgggt agcggtgcga ggctgtctgt caggtgacta atggcatcat
    18841 caagccgatc attaagcgcc tgatgcattt gcgaaatggc acgactggga aaccacagca
    18901 cgccgattaa cgtcatcagc aaggttggat agaggccaac aacgatacac cacagcgtta
    18961 agcgcacgac cacttccgga tagtcgagca tggcggggaa ggtttgcccg taaatggcga
    19021 caatggcgac agcgaaaaag accagtccca gccgatgggt gcgcatcaaa aacatgcagc
    19081 ccatcaggat cggcccggcg atgatcaatc ggatcaacgg ttcgccgtat gaccatttgt
    19141 agatcaaaaa caggctgccg atctccagca ccgtggcaac cacaaacaag atcgccacaa
    19201 atttggtgta aaacgcgttc gactgaatac cgtaaaacag cactgccagc gataacgcca
    19261 caaaagggat ctcaaaagtc atcgagatca gaatcaccag caggcagcct acccagagtt
    19321 gcacgatctg cggcacgcgg ccgggtcgcc gctcgcttaa ctcttcatga aagaacgcca
    19381 gcagcctgac caccggtaat ggcagggagt tgagcgcgct cattattgcg gctcaagatt
    19441 agcgactgcc gaagcgccga tgcggaacat ttccgggtca ggtttatcga ccatgatttt
    19501 gaccgaaaaa cgctgggcaa cgcggaccca gttaatagag cgagaaactt tcggcaggcc
    19561 gcccagcacc aggccgccgt cattcggtag cacgccgtag ccaatcgaat ccactttacc
    19621 ctcgaaggtt ttgccgctgt cactcatcag gcgaatcgtt gcgggtgtac ctgagcgaat
    19681 atttttcaga tcggtttcgc ggaagttggc gatcacgtac cagtgacgag tgtcgattag
    19741 ggtaaaaata gggcgcatgg cagaagcaaa ttgcccgacg gaggttttga gggaaatgac
    19801 ccggccatca aacggcgcgc gaacggtcgc catttccaga tgcagtttgg tcagggcaat
    19861 atccgcttcg accgccgcac gctgggcaac taatacatcc acgccgctga cggcgctggc
    19921 ggctgactgc gcctgtaaca atacggcatt aagatccgct tctgcggcgc gctgcgccgt
    19981 tcttgcacgg tcaacatctt ccgctgagac aaaaccttct ttcagtaatg gctcggtgcg
    20041 gcgtaatgta tctgtggcct gtttcgcggc ggcacgtgct ttttctaccg tggcattaac
    20101 cgagtcggca ccaaactgtt gggcgtcaac gctacgctgg gtgagcataa tttgcttatc
    20161 cagcgccgcg agggaggctt cagctttcgc cagattggct tcgtacgggc gcgggtcaat
    20221 gcggaacagc aaatcgccct gtttgactgc ctggttgtcg gtgaccccag ttctacaatg
    20281 cggccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc gtcattagtt
    20341 gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc caacgcaacc
    20401 actaacagag cagggaattt actgcgagga gcttttttcg gcgtactttc catgagcgcg
    20461 tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagacagc aaaaagggcg
    20521 gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataat tcgcctcgtg
    20581 attagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata ataagcgccg
    20641 atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg aattatccag
    20701 gctgtttttt tgagttgcat cgccatgcga gagagaactg ttggcatatt aaatacatcc
    20761 tgtttgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg tcagtatggc
    20821 tattttcttg atgaataaaa atagctttat tcagccaccg cattcataac ttatatattt
    20881 tatgtattaa ttgattgtat gtatttttat aataaatgct tttgggggct taagcgcccc
    20941 caaaagtgag tggcattaat ttggggttac gatattcacc cacaaatcga aattatccag
    21001 acaggcaata atttcttcca gtttcgcgcc attgccaata attttggctt tcttcgcttt
    21061 taccagatcc gccattttgg cttgtcctgt cagcacggcg tgcagatctt cacggctgat
    21121 gtagaaagag gcgtcggctt gcggttgcag tgttttacgg tagttaagca cgctatcgtt
    21181 cagcgtcagg ttgaggttat cgccgttgct catattgaag ttcaggctga tatttttacc
    21241 cgcagctttc gcgctatcga ggcgaacgga cataaagtcg aacagcattt cgaccgacat
    21301 cccgcgaatg gtgtccgggg aaccggtggt gccgtggctg aacttatgca ccccttcgcg
    21361 cagctctttc gcgccggtca ggtagaaacc gcgccaggtg gcggactcgg cctgatagcc
    21421 cagctgttca aagttattcg cttgcagatt cttcgcgacc tggtcacccg ggttggcggc
    21481 aatcacctgt ttcagcagtt ctgccgacca gcggtaatca ccttgcttgt tcgcttcttg
    21541 cgccaggctg atgacacggg cagaaccgcc cagcgcctgc acgtaacgtt tacccatctc
    21601 cacctgacca tacggatgca ggttagccgg gttaccgtcg taatagccaa gatagaagtt
    21661 atacaccgcg cgggcgttgt ggctgacaga accgtaatag ccacggctgg cccagttatt
    21721 ggcaagtgca ggcggcagtt taatcatgtc gccgatttca ttcatggtgt agccctggtt
    21781 cgccaggtgc agggtctggt cgtgaatgta cttgatagta tcgcgatatt taccaatata
    21841 atcattgata tgcttattgc cccggaccgg ccaggtgtgc ggcataaaca ggacttccgc
    21901 gtcgttaccc cacatatcca gcgtttcgtt cagatactcg gtccacttgc tggtgtcgcg
    21961 ggttttcgcg ccgcgcagag tgtagaagtt gtgcagggta tgcgtggcgt tctcggcggt
    22021 acacagcgct ttcagggccg gaatatagaa gtgcatttcg gctggtgctt cgctacctgg
    22081 ggtcatcagg aagtcaaact ccaggccgtc gataatcatc ttctcaccgg ttttggtgat
    22141 ggttttgttc ggtgcgatga tgctcgggta gcccgatgcc agcgttacgc caagaccatt
    22201 gccgatgtta ccctgagggt tatgcgccag cagcaggccg taggagtaaa gtgcacggcg
    22261 gctcataata ttacccgcca gtacgttttc gctgatggct tcgtccataa agccagcagg
    22321 ggcgataacc tgcactttac cggatttcac atcagcttca gagataatgc ctttcacgcc
    22381 gccatagtgg tcggtatggc tgtgggtata aataaccgca acaatcggtt tttgcgggcg
    22441 attttggaag taaaggtcaa gggcggcttt tgcgctcggt ggcgtcacca gcgggtcgat
    22501 gacaataatc ccggtatcac cttcgataaa ggtgatgttg gagatatcct gaccgcgaac
    22561 ctggtacatt ctgtcggtga ctttaaacag gccggaaata ccgtttaact gcgactgacg
    22621 ccacaggcta gggttaacgg tttcgggcgc gggcgcattg atatcaaatt tataatcttc
    22681 cccgcgataa tatggtttgc cattagcatc gcgcagaata ccttcatcca gcagcggagc
    22741 gataaatcca cgctgagcgt cgtcaaaatc ctgacgatcg ctaaacggta atgatttggc
    22801 gtaattctga ttaacctgtt gggtatattg agtggcggct ttcgcttcct ctttcgcgtt
    22861 agcggtattt accatcatgc cgctggcggc agttaacgca ataacaattc tgctcaaacg
    22921 gaataaccga gagttattca tataaatctc catttatcat ttatgtcgta aatatgtaat
    22981 ccatacaaaa tacgtcattt aaagctgggg ttatcataat tgggaatttg cgactttaat
    23041 aagtggaagt gtgagctgaa cgatccattt tattaggcat ttgtgctgaa gagagaaaat
    23101 t
//
LOCUS       DAJTMK010000053        23090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTMK010000053 DAJTMK010000000
VERSION     DAJTMK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23090
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.054"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..198)
                     /gene="pspE"
                     /locus_tag="OO135_001746"
     CDS             complement(<1..198)
                     /gene="pspE"
                     /locus_tag="OO135_001746"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5397630.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVY"
     gene            complement(273..494)
                     /gene="pspD"
                     /locus_tag="OO135_001747"
     CDS             complement(273..494)
                     /gene="pspD"
                     /locus_tag="OO135_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5397631.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(503..862)
                     /gene="pspC"
                     /locus_tag="OO135_001748"
     CDS             complement(503..862)
                     /gene="pspC"
                     /locus_tag="OO135_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5397632.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(862..1086)
                     /gene="pspB"
                     /locus_tag="OO135_001749"
     CDS             complement(862..1086)
                     /gene="pspB"
                     /locus_tag="OO135_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5397633.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1140..1808)
                     /gene="pspA"
                     /locus_tag="OO135_001750"
     CDS             complement(1140..1808)
                     /gene="pspA"
                     /locus_tag="OO135_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5397634.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            1975..2952
                     /gene="pspF"
                     /locus_tag="OO135_001751"
     CDS             1975..2952
                     /gene="pspF"
                     /locus_tag="OO135_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5397635.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHLGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3072..4337)
                     /gene="puuE"
                     /locus_tag="OO135_001752"
     CDS             complement(3072..4337)
                     /gene="puuE"
                     /locus_tag="OO135_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5397636.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENALKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4375..5655)
                     /gene="puuB"
                     /locus_tag="OO135_001753"
     CDS             complement(4375..5655)
                     /gene="puuB"
                     /locus_tag="OO135_001753"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5397637.1"
                     /translation="MTEHTSSYYAASANKYVPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTAPMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFHAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(5657..7144)
                     /gene="puuC"
                     /locus_tag="OO135_001754"
     CDS             complement(5657..7144)
                     /gene="puuC"
                     /locus_tag="OO135_001754"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5397638.1"
                     /translation="MNFHHLDYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7419..7976)
                     /gene="puuR"
                     /locus_tag="OO135_001755"
     CDS             complement(7419..7976)
                     /gene="puuR"
                     /locus_tag="OO135_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5397639.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAICTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTITGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8003..8766)
                     /gene="puuD"
                     /locus_tag="OO135_001756"
                     /pseudo
     CDS             complement(8003..8766)
                     /gene="puuD"
                     /locus_tag="OO135_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            8978..10396
                     /gene="puuA"
                     /locus_tag="OO135_001757"
     CDS             8978..10396
                     /gene="puuA"
                     /locus_tag="OO135_001757"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5397640.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLYGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQILLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10545..10709
                     /locus_tag="OO135_001758"
     CDS             10545..10709
                     /locus_tag="OO135_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397641.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPSPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            10699..12084
                     /gene="puuP"
                     /locus_tag="OO135_001759"
     CDS             10699..12084
                     /gene="puuP"
                     /locus_tag="OO135_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5397642.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12218..12463
                     /locus_tag="OO135_001760"
     CDS             12218..12463
                     /locus_tag="OO135_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5397643.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            12776..14419
                     /gene="sapA"
                     /locus_tag="OO135_001761"
     CDS             12776..14419
                     /gene="sapA"
                     /locus_tag="OO135_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5397644.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLHR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14416..15381
                     /gene="sapB"
                     /locus_tag="OO135_001762"
     CDS             14416..15381
                     /gene="sapB"
                     /locus_tag="OO135_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5397645.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15368..16258
                     /gene="sapC"
                     /locus_tag="OO135_001763"
     CDS             15368..16258
                     /gene="sapC"
                     /locus_tag="OO135_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5397646.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16258..17250
                     /gene="sapD"
                     /locus_tag="OO135_001764"
     CDS             16258..17250
                     /gene="sapD"
                     /locus_tag="OO135_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5397647.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKVNVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17252..18058
                     /gene="sapF"
                     /locus_tag="OO135_001765"
     CDS             17252..18058
                     /gene="sapF"
                     /locus_tag="OO135_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5397648.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18126..18479
                     /locus_tag="OO135_001766"
     CDS             18126..18479
                     /locus_tag="OO135_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5397649.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            18847..19635
                     /gene="fabI"
                     /locus_tag="OO135_001767"
     CDS             18847..19635
                     /gene="fabI"
                     /locus_tag="OO135_001767"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5397650.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            19780..20907
                     /locus_tag="OO135_001768"
                     /pseudo
     CDS             19780..20907
                     /locus_tag="OO135_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            20975..22909
                     /gene="rnb"
                     /locus_tag="OO135_001769"
     CDS             20975..22909
                     /gene="rnb"
                     /locus_tag="OO135_001769"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5397651.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(22987..>23090)
                     /locus_tag="OO135_001770"
     CDS             complement(22987..>23090)
                     /locus_tag="OO135_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397652.1"
                     /translation="TYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
BASE COUNT         5199 a         6129 c         6016 g         5746 t
ORIGIN      
        1 atacactttc acggtgtcgt ttttatccgg aacggcggtg gcaatgcgct ctttcacttc
       61 cttcagggga atattgatgg ccccctgaac gtgctcttgc tgatactgct ctggaacacg
      121 aacatcgatc cagtgttcag cggcgaaaac gggcagtgaa aacaccagtg ccagagcaag
      181 taagcctttt ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg
      241 cctgagattt aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta
      301 ttagcagccc gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg
      361 cgagcaactg attttatcgc ccagcccgct acacccgccg ggccatagcg cagtgcggta
      421 agaagtacca gcttgcctgc taatttgaaa ccaggcttta ccttttgccc ggcctgttgc
      481 cagcgagtat tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat
      541 cggaagtgac ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt
      601 cgacttcatc cagcaattcg ctgctggagg gtagctgctc accaaaggcc atgttgtccg
      661 gcattggatc aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac
      721 cgaagaaaat cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt
      781 tggcaatccc ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataattttt
      841 tattgagatt aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat
      901 agattccagc gcctgaatac gttcgcgcat ccgttttgct tcatcagcca gttgcgctaa
      961 tcgctgctgc tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca
     1021 taaccagatc ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc
     1081 gctcataagt actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat
     1141 tattgattgt cttgcttcat tttggctttt aattgtgcca gttgttcact gattgcatca
     1201 tcggctttca gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt
     1261 tctgcttccg cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct
     1321 tcatccagtt tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga
     1381 tggcgtaaca tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctca
     1441 ccaatctctt tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccaga
     1501 ctcttaatca gatcggtcag tttctgtttt tcaattaacg ccgcacgtgc caaatcctct
     1561 ctctctttca gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct
     1621 tgttcaatac ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt
     1681 acttcaacca gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct
     1741 tccgctttct ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa
     1801 atacccataa tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat
     1861 acaatttgcg tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcatc
     1921 agcaacatgc caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggca
     1981 gaatacaaag ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt
     2041 tcgcatctcg caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa
     2101 gagctgattg ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc
     2161 cttaactgcg cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg
     2221 ggggcgttta ccggtgcgca aaaacgtcat ctagggagat ttgaacgtgc cgacggcggt
     2281 acgctatttc ttgatgaact cgctacggca ccgatgatgg tgcaggagaa attattgcgc
     2341 gtgattgagt acggtgaact ggagcgcgtt ggcggcagcc agccattgca ggtgaatgtg
     2401 cggttggtat gcgcgacgaa tgccgatctc ccggcgacgg tcaatgaagg cacttttcgc
     2461 gctgacctgc tcgaccgact ggcttttgat gttgtacaac tgccaccact gcgcgagcgc
     2521 gaaagcgaca taatgttgat ggcagaacac tttgccatcc agatgtgtcg ggaaatcaag
     2581 ctgcctctgt tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg
     2641 ccgggaaata ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc
     2701 agcgattatc cgcttgatga catcattatt gatcccttta aacggcgtcc gcctgaagac
     2761 gctatcgccg tttcagaaac cacctcgctt ccaacactgc cgctggattt acgtgagttt
     2821 cagatgcagc aggaaaaaga gttgctgcaa ctcagtttgc aacaagggaa atataaccag
     2881 aagcgcgcag ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag
     2941 caccagattt agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc
     3001 ggcatacata atcaataagt cgtttgcagg cctgataagc gcagcgcatc agacattatt
     3061 gcgttgggct attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc
     3121 gcatccggga tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc
     3181 agcaggagca gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac
     3241 ggctcgcccg tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc
     3301 gcagcaatgg ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc
     3361 agttgattcg cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg
     3421 gcagccaccg ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg
     3481 tccataatat tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc
     3541 gccatcgtca ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta
     3601 cgcgcaaagc cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg
     3661 cggcgaatgg cggcaaccag ctcttttggc gcaacgttga aaccgccctc gccctgcacc
     3721 ggttcgaaaa taatggccgc cacctgcttc gcttcaatgt ccgatttaaa caagcgttcg
     3781 atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga
     3841 tacaccgaac cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc
     3901 gccatggtca tatatgtacg accgtggaag ccgccgctaa acgcaatcac gccaggtcgt
     3961 ccggtatggg cgcgagcaat tttcaacgcg ttttccaccg cttccgcgcc ggtagtgaag
     4021 aatgcggttt tagcctgccc gctcactggg gcaagggcgt taattttctc cgccagcgtg
     4081 acgtagcttt cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc
     4141 tccaccgccg cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg
     4201 gcgaaatcga tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg
     4261 gcgaagaagt tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg
     4321 aattcattgt tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac
     4381 gcccagacga tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag
     4441 cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg aaggcatgga aacgctcggc
     4501 gtcaccgcgc agcagttcag caatcaaacg cccggcgagg tgggtacagg tgacgccgtg
     4561 gccgctgtag ccctgcatgt aatagatgtt ggtatcgagg cgaccgaact gcggcatccg
     4621 tgaaagggtc agcaggaagt tgcccgtcca gcgatagtcg attttgacgc ccttcagctg
     4681 cgggaaggtt ttcagcagtt tcggcacaac aagacgctca acgtcatccg ggtcgcgcgc
     4741 gccgtagacc acgccgccgc cgtacagcaa acggttgtcg gcggtaagac ggtagtaatc
     4801 aagcagataa ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc
     4861 ttccgacagc cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgccag
     4921 ttccggctct actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt
     4981 cacctgacct ttggcagtgc gcacaacggc tggcgtggtg tgctggatct gcgtcacggc
     5041 agaaagttca tacacgcgcc cgccgttgag gcggatggcg tccgcttcgc caatggcgag
     5101 gtttagcggg tgaatatgcc caccgctgtg atccagcagc gcaccggtat agcgatcgct
     5161 ggctacttca cggcgaatgg cgttggcgtc cagcaattcc agctgtttat tgccgtagcg
     5221 ttcccagttc tctttctgct cttcaagtgt ggcgagctgt ttatcattca tcgccacaaa
     5281 caggccgccg gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat
     5341 ctcaccacct tcgaacatca tgctgccgag cataggggcg gtgtccatgc cgtagctttt
     5401 ttcgatcacg tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct
     5461 tgcgccaaag ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag
     5521 atgcagcgcg gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat
     5581 tgactcattc agggtgtcga atggcacgta tttattcgca ctggcggcgt aataactgct
     5641 ggtatgttcg gtcatttcag gcctccaggc ttatccagat ggttttcagt tcggtgaatt
     5701 tttcaagggc atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccaccaaacg
     5761 gcacggtcat atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcggc
     5821 ggctcatacg gtgcgcgcgg gagaggtcgc gcatccatac cgccgcgcca aggccgtact
     5881 ggctgtcgtt ggcaagctgt agcgcctgtt cttctgatgt gaaacgcgtg accaccagca
     5941 ccggaccgaa aatctcttcg cggcttaagg acgcattcgg gtctacatcc acaaagatgg
     6001 tcgggccgat ggcggcagcc agcccggcgt tacggccatc cagcagcagt tgccctttgc
     6061 cttcgccctc acgaataaag ctatggaccg agtcggcgtg ggcgcagtcg attaaagtgc
     6121 ccatggtggt tgcggggtca agcggatggc ccggctgcca gttttgcgcc tgctgtttta
     6181 acagggctaa gaattcatcg gcgatgctct cttccagcaa caggcgtgtt ccggcgatgc
     6241 acacctgacc ctggttgtag aagatgcctg ctgcggtgga gctcgccgct ttttgcagat
     6301 ccgaacaatc ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacgcgtt
     6361 tcatgttgct gtcgcccgca tctttcagca gctgtttccc ggtacgggtt gaaccggtaa
     6421 aggcaatggc gtcgatatcg ttatgacgcg acagcgcctg ccccgcttca tgaccaaaac
     6481 ccgtcaccac gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac
     6541 gaatcgcact gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
     6601 gcgccgggcc gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg
     6661 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
     6721 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
     6781 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
     6841 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
     6901 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
     6961 cacggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg
     7021 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
     7081 agcgattttc aatggcgaga cttaacgctt tatcctgcca gtaatccaga tgatgaaaat
     7141 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
     7201 gagctccccg ggccgtatgc ccgtttattt ctggcgacat tctttatata cacttcatcc
     7261 ttcaagccgc ctctgccttg gctgcgttcg ctcccccgaa tcacttacct gagtaagctc
     7321 atcgggattc gctcacttgc cgccttgatg caacttgaat gattttgtgt atagattgcc
     7381 gggcgcattg cgcttacccg gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg
     7441 gcgctgataa ttcggcaaat acctgccgac gtattactga aactgtgcgg gatgccggta
     7501 ttaatggcat aactttgacc cgcgacgagg tggtaatcct gaccattaat cgtcagaaca
     7561 atttcacctt ccagtactgt gcctatttcc tcaccctgat gcttaattct ttccccagtg
     7621 attgtgcccg gctggtacgt ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta
     7681 tgaaccagct tcattgacac accctgactg cccatctcaa ttaagtcgtc ctgattaatg
     7741 acgacctgcg gctcatcagg tttttccggc tcggaaaaga attccgagag tgacagacca
     7801 tacaccttca gcagcttttg cagcgtactg atggcaggac tgactttatc ttgttctatc
     7861 gtacagatag cactgtgagt cagcccggag agttcggcgg cacgacgttg tgaaagcccc
     7921 tgctgctggc ggatttccga caagcgtttt cctggcgcca gtccctcatc actcatattt
     7981 gcatttcctt aaactgtagt ggtcagagtc gctgtttttc agcgatatgg tgctgacaag
     8041 cggtgataaa gccctcgaac aatatacgcg aaagcgcgta ctcgctactg ttccattccg
     8101 ggtgccactg tacgcccagc gcaaaaggat gattgatgac gctaaccgcc tcaactaagc
     8161 catccggcga gcgagcttca acacgcaacc gtgggctaac gaccttcgcc ccttgtccat
     8221 gtagagagtt tacccaaaag ttgctacatt caggtaacaa cgcagacagt aatcccccct
     8281 cttcaacctg aacttcatgc gacggtgcat attgctgttc caccggcagt tcggatcttc
     8341 ccgatgttcc agcaattcag gctgttcgca cagcttgcga tgcaacgaac caccggttgc
     8401 caccaccagt tcttgtaaac cccggcagat ggcgaaaatg gggatgcgcc tttcgagtgc
     8461 ggcattaatt atcgccatgc tcagaagatc acgcccggga tcggcgtcag gctcatcgcc
     8521 gttttcacca tataggtgcg gctgcacatt gctgggacta ccaggaagat aaatgccatc
     8581 gagtttcggc aaaagttgtt caagtaacga cggttccgcc agcgcatgtg gtagcgcaat
     8641 tggcaagccg cctgcgtgga tgatggcatt caggtacttt tcttgcagag tctgggtcgc
     8701 atgaccctta agcctgttcc tgcacattac gacaccgata accggattgt tcattatatt
     8761 ttccatgctc aatctcacaa agtggactaa attatcgcca ttactgcgct ttaaatgacc
     8821 tttatgttca atattttttc aatctagcag ttggttttca tttttgcaaa ctaaatttaa
     8881 catttgacga acatttagtt tgcatacaga ttcgaatggt ggtcattata ttttacgctt
     8941 tgataacgag cggaaaacaa accaaaggcg aagaatcatg gaaaccaata tcgttgaagt
     9001 agagaacttt gttcagcagc cagaagagag gcggggtagc gcctttacgc aggaagtgaa
     9061 acgctacctg gagcgctacc cgaatacgca atatgttgat gttttactta ctgatttgta
     9121 tggttgcttc cgtggaaaac gtatccctgt ttcaagcctg aagaagttgg agaaagggtg
     9181 ttatttcccg gcctcggtgt ttgcaatgga tattctgggc aacgtagtag aagaagcggg
     9241 tctgggtcag gaaatgggcg agccggatcg tacttgtgtt cctgttctcg gttccttaac
     9301 cccttctgcc gccgatccag agtttatcgg tcagattctc ctgaccatgg tcgatgaaga
     9361 tggcgctccc tttgacgttg agccgcggaa cgttcttaac cgcctgtggc agcagctacg
     9421 ccagcgcgga ttgttcccgg ttgtagcggt agagctggag ttctatttac tggatcgtca
     9481 gcgcgacgct gaagggtatc tgcaaccgcc ctgcgcgccc ggcaccgatg accgcaatac
     9541 acaaagccag gtttactccg ttgataacct caaccacttc gccgacgtgc tcaatgatat
     9601 tgatgaactg gcgcagttac agttaattcc cgccgacggt gcggtcgccg aagcctcgcc
     9661 gggtcagttt gaaatcaacc tttaccatac cgataacgtg ctggaagcct gcgatgatgc
     9721 gctggcgcta aaactcctgg tgcgtctgat ggcagaaaag cataagatgc acgccacttt
     9781 tatggcgaag ccgtatgaag agcacgcggg cagcgggatg catatccata tcagtatgca
     9841 aaataatcgt ggcgagaatg tgctttctga cacagaaggc gaagattcgc cgctgctgaa
     9901 aaagatgctc gccgggatga ttgacctgat gccgtcgtcg atggcgttgc tggcaccaaa
     9961 cgtgaactcg tatcgccgct tccagccggg aatgtatgtg ccgacgcagg cgtcgtgggg
    10021 ccataacaac cgcaccgtcg ccctgcgtat tccgtgcggc gaccgtcata atcaccgcgt
    10081 ggaatatcgc gtggcgggtg ccgatgccaa cccatatctg gtgatggcag cgatttttgc
    10141 cggtattttg catggccttg ataacgagct gccgttgcag gaagaagtcg aaggcaacgg
    10201 gctggaacag gaaggcttac ccttcccgat tcgccagagc gatgccctgg gtgagtttat
    10261 cgagaatgat cacctgcgcc gctatttagg cgaacggttt tgccatgtgt atcacgcctg
    10321 taaaaatgat gagctgttgc agtttgagcg cctcatcaca gaaacagaaa ttgagtggat
    10381 gttgaaaaac gcatagtacc ccttgcgggg ttgtttcatc gacctgagcg gagccaggcc
    10441 tgacatttgc tggtaatcta gacatttccc ggatgtcacg ttatggagcg caggcggcaa
    10501 cgggcttccg ttaacgatta agactgtgac gaggaaatga gatgatgccg atgattaaat
    10561 ccccgcatgg cgagggaggc tgcgtatgcg cccctcctgc tactgactgg actccgtccc
    10621 ctttgcttcc cttgctaaac aggtttgatt tcaggtcgac acgaccgcaa accttattac
    10681 gcaggggagg cagcaattat ggctattaat tcaccactga atattgctgc gcaacccggc
    10741 aaaacccgtc tgcgaaaatc actgaaattg tggcaggtgg tgatgatggg tctggcctat
    10801 ctcacgccga tgaccgtatt tgatactttt ggtattgtct ccggcattag cgacggtcac
    10861 gttccggcgt cctatttgct ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc
    10921 aaactggttc gccagtttcc ggaggccggt tcggcctata cctacgcgca aaagtcgatt
    10981 aacccgcacg tcggatttat ggtcggctgg tcatcgctgc tggattatct ctttttgccg
    11041 atgatcaacg tcttgttggc gaaaatctat ctctccgccc tcttcccgga agtgccgccg
    11101 tgggtgtggg tagtaacctt cgtcgccatt ttaaccgccg cgaatctgaa gagcatcaac
    11161 ctggtcgcta acttcaatac cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt
    11221 atcttcctgg tagttcaggg actgcataaa ggagaaggcg ttggcaccgt ctggtcactt
    11281 cagccgttta tcagcgagaa cgcgcacctg atcccgatta ttaccggggc gacgattgtc
    11341 tgtttctcct tcctcggttt cgatgcggtg accacgcttt cggaagagac gccggacgcc
    11401 gcacgcgtga tcccgaaagc catcttcctg acggcggtct acggtggcgt tatctttatc
    11461 gcggcgtcgt tctttatgca gctgttcttt cccgatatca gccgctttaa agatccggat
    11521 gccgcactgc ctgaaattgc gctgtacgtc ggcggcaagc tcttccagtc gattttcctc
    11581 tgcaccacgt ttgtgaacac gttagcctct ggcctggctt cacatgccag cgtgtcgcgt
    11641 ctgctgtatg tgatggggcg tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac
    11701 ccaaaatggc ggactccggc actcaacgtc attatggtcg ggattgtcgc gttgtcggcg
    11761 ctgttcttcg atttagttac cgccacagcg ttaattaact tcggtgctct ggtggcgttt
    11821 accttcgtga atctgtcggt gtttaatcat ttctggcggc gtaaaggaat gaataaaagc
    11881 tggaaggatc acttccacta tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg
    11941 ctgtgggtta acctcgagtc aacgtcactg acactcggtc tggtatgggc ttcgttaggc
    12001 ggcgcatatt tatggtatct gatccgccgc tatcgcaaag tgccgttgta tgatggtgac
    12061 agaacgccgg tgagtgaaac gtaatagtcg gatgcgcggc gcattatcct gcctgagggt
    12121 aatgcaccgc gccattaact ataaaaagat gtatgacccg gatcacgagt gctatcatcc
    12181 gcccttatgc ttagcgcatt aaccccaggg gcaaataatg aaccacgata tcccgctaaa
    12241 atattttgat attgccgatg agtacgcgac cgaatgcgca gagcctgtcg cagacgctga
    12301 acgcacaccg ctggcccact acttccagtt actgctcacc cgtttgatga acaatgaaga
    12361 gatcagcgaa gaagcccaac atgaaatggc tgctgaagcg gggattaatc ccgtacgcat
    12421 tgatgagatc gcagagtttt tgaatcaatg gggtaatgag taattattta attgcagacg
    12481 atcggtcagt atcgttgtcc ggataaagcg ttcacgccgc atccggctaa caatgacacg
    12541 ctctgcagcg tgctttaatg ggtttctcac gatcgtaccg ctctgctcct cgctaagttg
    12601 ttctgtaagt tatagttata gagaaagcac gggtacgaat gttgaacaga tggcgacgcc
    12661 acctgttcaa cgaccaacca cacggccctc attacgaccc ctgaacacaa acggtaatgg
    12721 caaatgtcat taaatattag taagatagcc tctttacata aaatccctta atattatgcg
    12781 ccaggtatta tcgtctcttt tggtgattgc tggacttgtg agtggtcagg caatcgccgc
    12841 gcctgaatcc cccccgcatg ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg
    12901 gcaagtcaac acctttaacc catccaaagc gagcagtggg ttaattgtcg atacccttgc
    12961 cgcccagttt tatgatcgac tgctggatgt cgatccctat acctatcgcc tgatgccgga
    13021 acttgccgaa agctgggaag tactcgacaa cggcgcgacc tatcgcttcc acctgcatcg
    13081 cgatgttccg tttcaaaaaa ccgactggtt tactcccact cgtaaaatga atgccgacga
    13141 tgtggtgttt accttccagc gaatttttga ccgcaacaac ccgtggcata acgtcaacgg
    13201 cagcaacttc ccctacttcg acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa
    13261 actggataat cataccgttg agttccgact cgcccagccg gatgcttctt ttttgtggca
    13321 cctcgcaacc cattatgctt cggtcatgtc ggcagaatat gcccggaagt tagagaaaga
    13381 agatcgccag gagcaactcg accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga
    13441 ataccgcgcc gggcaattta ttcgcctaca acgtcatgat gacttctggc gcggtaaacc
    13501 gttaatgccg caggtagtgg tggatttagg ctccggcggc accggacgtc tgtcgaaact
    13561 cctgaccggg gaatgcgacg ttctggcctg gcctgctgcc agccagctat ccattttgcg
    13621 tgacgacccg cgcttgcgtt taacgctgcg tcctgggatg aacgtcgcct atctggcatt
    13681 taacaccgcc aaaccgccgc taaataatcc cgctgtccgc catgcactgg cactggcgat
    13741 taataaccag cgcctgatgc aatccatcta ttatggtacg gctgaaacgg cggcctctat
    13801 tttaccgcgc gcctcgtggg cctatgacaa cgaggctaaa attactgaat acaatccggc
    13861 gaaatcgcgc gaacagttga aggcgttggg gctggaaaac ttaacgctga aactgtgggt
    13921 gcccacacgt tcgcaggcgt ggaaccccag tccactgaaa actgccgaac tgattcaggc
    13981 ggatatggcg caggttggcg taaaagtggt gattgtgccg gtagaaggtc gctttcagga
    14041 ggcgcggttg atggatatga gccatgatct gacgttatcc ggttgggcga cggacagtaa
    14101 cgacccggac agtttcttcc gtcctttact gagctgcgcg gcaattcatt cacagaccaa
    14161 cctcgcccac tggtgcgatc cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca
    14221 gcagctggcg gcgcgtattg aagcctatga cgaagcgcag agtattctgg cgcaggaatt
    14281 gcccattttg ccgctggcgt cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg
    14341 tctggtactt agcccgtttg gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga
    14401 tgaggtgaaa aaaccatgat tatcttcacc ttacgccgca ttttgttatt gattgtcacc
    14461 ttgttcctgc tgaccttcgt tggcttcagc ttgagctatt tcacccctca tgcgccgtta
    14521 caaggcgcgt cactgtggaa tgcctgggtg ttctggttta acggcctgat ccactgggat
    14581 tttggtgttt ccagcattaa cggtcaaccg attgccgaac agttaaaaga ggtcttcccg
    14641 gcgacgatgg aactgtgcat cctcgccttc ggttttgcgc tgattgttgg catccccgtg
    14701 gggatgattg ccgggattac gcgccataag tggcaggata acttgatcaa cgccatcgcc
    14761 ttactgggct tttcaatccc ggtgttctgg ctggcgcttc tgttgacgct gttttgttca
    14821 ctcacgctag gttggttgcc cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg
    14881 attaccggtt ttgcgttgat tgatgcctgg ctttcggact caccatggcg ggatgaaatg
    14941 atcatgagcg caatccgcca tatgatattg cccgtgatca ccctgtcggt tgcgccaaca
    15001 actgaagtga tccgcctgat gcgtatcagc actatcgaag tgtatgacca gaactatgta
    15061 aaagcggcgg cgacccgcgg tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat
    15121 aacgcgttac cgccggttat tcctcgcctg ggcttacagt tttccaccat gctgacgctg
    15181 gcgatgatca ccgaaatggt ttttagctgg ccgggtttag ggcgctggtt aattaacgcc
    15241 atccgccagc aggattacgc agccatttcc gccggagtga tggtgtgtgg ctcactggtc
    15301 attattgtta acgtgatttc tgatattttg ggtgccatgg ctaaccctct gaaacataag
    15361 gaatggtatg ccttacgata gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac
    15421 cgcctggcgc aaattttata gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg
    15481 gctggctgta ctgtgtattt ttggcggctg gtttgcgcct tacggcatcg accagcaatt
    15541 tctcggttat caattattgc cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct
    15601 ggggactgac gacctcggac gcgatgtttt aagccgttta ctgagcggag ctgcgccaac
    15661 cgtgggtggc gcatttgtgg tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac
    15721 cttcgccggg gcgacacacg gcctccgctc agcggtgctt aaccatattc tggatacctt
    15781 actggcgatc ccttcgctgc tgttggcaat tatcgttgta gcgtttgccg gaccgagttt
    15841 gtcccacgct atgtttgctg tctggctggc attgctgccg cgtatggtac gttcgattta
    15901 cagcatggtg catgacgaac tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc
    15961 atcaacgctg aatattctct ggtttgctgt gatgccaaac atcaccgccg ggctggtcac
    16021 cgagatcacc cgcgcactgt cgatggcaat tctcgatatc gccgcgctgg gctttctcga
    16081 tctcggcgca caactcccct cgcctgaatg gggagcgatg ctcggtgatg cgctggaact
    16141 gatttatgtc gccccgtgga ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt
    16201 gttggttaac ctccttggcg atggcgttcg tcgtgcgatt attgcggggg tggaataatg
    16261 ccattactcg atattcgtaa cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa
    16321 gccgtcgacc gggtaagtat gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa
    16381 tccggttccg gcaagagttt gattgcgaaa gcgatttgtg gggtgaataa agataactgg
    16441 cgtgttactg ctgaccgtat gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc
    16501 gaacggcgca aactggttgg ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt
    16561 cttgaccctt cagaacgtgt gggtcgccag ttgatgcaaa acatcccagc ctggacctat
    16621 aaaggccgtt ggtggcagcg ttttggctgg cgcaaacgcc gtgccattga actgctgcac
    16681 cgcgtgggga ttaaagatca caaagatgcg atgcgcagtt ttccctatga gttgaccgaa
    16741 ggtgaatgtc agaaagtgat gatagccatt gcactggcga atcaaccgcg tctgctgatt
    16801 gctgacgaac cgaccaactc aatggagcca acaacccagg cgcaaatctt tcgcctgctg
    16861 acgcgtctca accaaaacag caataccact attttgctta tcagccatga cttacaaatg
    16921 cttagccaat gggcggataa agttaacgtg ctttactgcg gtcaaacagt ggaaaccgcg
    16981 ccgagtaagg aattggtgac gatgccacat catccttata cccaggcgct gatccgcgcg
    17041 ataccagact tcggcagcgc gatgccgcat aaaagtcgcc tcaatacgct gcccggcgct
    17101 atcccactgc tggaacagtt accgattggt tgtcgtctgg ggccacgttg cccgtatgca
    17161 caacgagaat gcattgtgac gccacgtttg acgggggcga aaaatcatct ctatgcctgt
    17221 catttcccgc tgaacatgga gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa
    17281 tttaagtaaa acgttccgct accggaccgg ctggtttcgc cgtcagaccg tagaagcggt
    17341 aaaacccttg agctttacgc tacgtgaagg ccagacactg gcgattattg gcgagaatgg
    17401 ttcgggtaaa tccacgctgg caaaaatgct ggcgggaatg atagagccta ccagcggcga
    17461 gttattgatt gacgatcatc cactgcattt tggcgattat tccttccgca gtcagcgtat
    17521 tcgcatgatt tttcaggatc cttcgacctc gttgaatccc cgtcaacgta tctcgcaaat
    17581 tctggatttt ccactgcgcc tgaacaccga cctggaaccg gaacagcgtc gcaaacagat
    17641 tattgaaacg atgcgtatgg ttgggctgct accggatcac gtcagttact atccgcatat
    17701 gttggcaccc ggacaaaaac agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa
    17761 agtcattatt gccgatgaag cactcgcttc actggatatg tcgatgcgtt cgcagttgat
    17821 taatctgatg ctggagttac aggaaaaaca gggcatttcg tatatttatg ttacccagca
    17881 tatcggaatg atgaagcaca ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt
    17941 cgagcgaggc agcaccgcgg atgtgctggc atcgccgctg catgaactca ccaaacggct
    18001 gattgccggg cactttggcg aagcattgac agcggatgcg tggcgtaaag atcgctaatt
    18061 ttctgaatcc tccttccaga ttatccacct cattctgaca agagattaaa tcttgtctga
    18121 atgaggtgat ggataaaata aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc
    18181 aggaacgaaa gctctggcga tatcttcgca gccgacgttt tggtgatttc aaatttcgcc
    18241 gtcaacatcc ggtggggagc tacattctcg attttgcttg ctgctcggcg cgcgtagtcg
    18301 ttgagctgga tggtgggcag catgatttgg cagttgccta tgataccagg cgcactagct
    18361 ggcttgagtc gcagggctgg accgtgctgc gtttctggaa taacgagatt gattgtaatg
    18421 aggaggcggt gctggagatt attctgcagg aactgaaccg ccgatcaccc tcaccctgaa
    18481 agggcgaggg ggccgaccga gccgaatagc tgttgtggtg aaaacatgga gacagtgctg
    18541 gagaatattc ggcaaggtct gaaccatccc agccatcgcc ataaaagggt taggggctgt
    18601 atgagcctgt ttgttgctgg ggtaacaata tttgcacaat atggtcccct cgcccctctg
    18661 gggagagggt tagtgtaagg ggaaaagcgc ccaccctgcc gcagcctgct ccggtcggac
    18721 ctggcaacta tagctactca cagccaggtt gattataata accatttatc tgttcgtact
    18781 gtttactaaa acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt
    18841 aaagctatgg gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaacta
    18901 tccatcgcct acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc
    18961 taccagaacg acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac
    19021 atcgttctgc agtgcgatgt tgcagaagat gccagcatcg acaccatgtt cgctgaactg
    19081 gggaaagttt ggccgaaatt tgacggtttc gtacactcta ttggttttgc acctggcgat
    19141 cagctggatg gtgactatgt taacgccgtt acccgtgaag gcttcaaaat tgcccacgac
    19201 atcagctcct acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt
    19261 tctgccctgc tgaccctttc ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt
    19321 atgggtctgg caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt
    19381 ccggaaggtg tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcttcc
    19441 ggtatcaaag acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt
    19501 accgttacta ttgaagatgt gggtaactcc gcggcattcc tgtgctccga tctctctgcc
    19561 ggtatctccg gtgaagtggt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa
    19621 ctcgaattga aataatcatt ctgttggtaa atattgggcg gcgttctgcc gcccgttatc
    19681 tctgttatac ctttctgata tttgttatcg ccgatccgtc tttctcccct tcccgccttg
    19741 cgtcaggata acgatttcct ttacgaccaa ggagcgccca tggaacaacg ccacatcacc
    19801 ggcaaaagcc actggtatca tgaaacgcaa tccagtactg cggagtatga cgttctgcct
    19861 ctggtcccgg aagccgcaaa ggtcagcgat ccctttctgc tcgacgtgat ccttgatgaa
    19921 gaaacgctgg ctcccttcct ttcatagctg gtccctgcgc gcgttcttgc agtggaattg
    19981 ttccctgacc agcttaccgt gacccgttca cagaccttca ccgcttatga acgcttgtct
    20041 acggccctga ctgttgctca ggtttgcggc gtccagcggt tatgtaacta ctattcggcg
    20101 cgacttacgc cgctccccgg gcctgattcc tctagggaaa gcaatcatag gttggcacaa
    20161 atcacgcaat atgcccgcca actggctagc tcgccttcta ttatcgacaa ccgatcgcac
    20221 cagcatctga atgacgtcgg tcttactgcc tgggactgtg tgttcattaa ccaaatcatt
    20281 ggttttattg gctttcaggc gcggaccatt gcgacacttc aggcttatct cgggcacccg
    20341 gtacgctggt tacccggtct ggaaatacaa aactacgccg acgcgtcact gtttgctgat
    20401 gaatcaatac gctggcgaag cagctatgag gtggaaaaac tacctgaaga gtacacaaaa
    20461 agttcaacag cagaactttg ccaactggcc gaaacactct ctctccaccc tatttcactt
    20521 tcccttctcg aaaggttgtt aaacagtaca cgggttaata cacagccgga taataagctt
    20581 gcggcgttgt tatgcgcgcg gataaatggc agtcctgctt gttttgccgc ctgtatggat
    20641 tcatcaaatg aatataaaaa aatcagcccc cttctgcgca agggcgaaaa tgaaattaac
    20701 caatgggctg accgtcattc tgttgagcac gctaccgttc aggcgataca atggctgacc
    20761 cgagcacccg atcgctttag caccgcccag ttcagccctt tactcgaaca cgaacaatca
    20821 tcaacgcaga ttattaatct gctggtatgg agcgggcttt gtggctggat aaatcgctta
    20881 aaaatcgcgt tgggtgagac atattaacct tgccgcgtca gacagattcg cgtaaaactg
    20941 tcagccgctc taatggccac caaaatagac aattatgttt caggacaacc cgctgctagc
    21001 gcagcttaaa cagcaactgc attcccagac gccacgcgct gaaggggtgg taaaagccac
    21061 agaaaaaggc tttggcttcc tggaagtcga cgcgcaaaaa agttatttca ttccgccgcc
    21121 gcagatgaaa aaagtcatgc atggcgaccg aattatcgcg gtgatccaca gtgaaaaaga
    21181 acgtgaatcc gcagagccag aagaactggt tgaaccgttc ctgactcgtt tcgtgggtaa
    21241 agttcagggc aaaaatgacc gtctggccat cgttcctgat catccactct taaaagacgc
    21301 cattccttgc cgcgcagccc gtggcctgaa ccacgagttt aaagaaggcg actgggcggt
    21361 tgccgaaatg cgccgtcatc cgctgaaagg cgatcgttct ttctatgctg aattgacaca
    21421 atacatcact tttggtgacg accactttgt accgtggtgg gttacacttg cgcgccataa
    21481 tctggaaaaa gaagcaccag acggcgtcgc taccgaaatg ctcgatgaag gtctggttcg
    21541 tgaagatctg accgcgctgg attttgtcac catcgacagt gccagcacag aagatatgga
    21601 tgacgccctt ttcgctaagg cgttgccgga tgacaaactt cagctgattg tggcgattgc
    21661 cgatccaacc gcgtggattg ctgaaggcag caagctggac aaagccgcga aaattcgcgc
    21721 attcaccaac tatctgcctg gcttcaacat ccctatgctg cctcgcgagc tttctgacga
    21781 tctctgctca ctgcgcgcca atgaagtccg cccggtactg gcatgccgca tgacgctctc
    21841 cgctgatggc gccattgaag ataatatcga attctttgcc gccaccatcg aatccaaagc
    21901 aaagctggtg tatgaccagg tttctgactg gctggaaaat accggtgact ggcagcctga
    21961 aagtgaagca attgccgaac aagtccgttt gctagcgcaa atttgccaac gccgcggcga
    22021 gtggcgtcat aaccacgcac tggtgtttaa agatcgcccg gattaccgct ttattctcgg
    22081 tgaaaaaggt gaagtgctgg atatcgtcgc cgagcctcgt cgcattgcca accgtatcgt
    22141 cgaagaagcg atgattgccg ctaacatttg tgcggcccgc gtactgcgcg ataagctcgg
    22201 ttttggcatc tataacgtgc atatgggctt tgatccggcg aatgccgacg cgctggcagc
    22261 gttgctgaaa acgcacggtc tgcatgtcga tgccgaagaa gtgctcacgc tggacggttt
    22321 ctgcaaactg cgtcgtgaac tggacgcgca accaactggt ttcctcgaca gccgcattcg
    22381 tcgcttccag tcatttgctg aaattagcac tgaacccggt cctcactttg gcctcggtct
    22441 ggaagcatac gccacctgga cttcgccgat ccgtaaatat ggcgacatga tcaaccaccg
    22501 tctgctgaaa gcggttatca aaggcgaaac tgcgacgcgc ccacaggatg aaatcactgt
    22561 ccaaatggcc gagcgtcgcc gtctcaaccg gatggcagaa cgtgatgttg gtgactggtt
    22621 atacgcacgc ttcctgaaag acaaagccgg gaccgacacc cgtttcgcag cggaaattgt
    22681 cgatatcagc cgtggcggca tgcgtgtgcg tttggttgat aacggcgcta tcgcctttat
    22741 tccggcacct ttcttacacg ctatgcgcga tgaactggtt tgcagccagg aaaacggcac
    22801 tgtacaaatt aaaggtgaaa cggtttacaa agtaactgac gttattgacg tcaccattgc
    22861 cgaagtccgc atggaaaccc gcagcattat tgcgcgcccg gtcgcgtaat ctcctttcac
    22921 ggcctattcc tcatggatgg gccgtttatt tccccgctct accttcgtta tatccaggca
    22981 gattttttaa ctgactttcg tttgaaaact ggcgtttttc caaaactgga ttacaaataa
    23041 atatataaaa aaaacaatgc gtttagccgc cgttaatttc atgtaagtca
//
LOCUS       DAJTMK010000054        23077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTMK010000054 DAJTMK010000000
VERSION     DAJTMK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23077
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.055"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            131..628
                     /gene="ftnA"
                     /locus_tag="OO135_001771"
     CDS             131..628
                     /gene="ftnA"
                     /locus_tag="OO135_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5397653.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(666..905)
                     /locus_tag="OO135_001772"
     CDS             complement(666..905)
                     /locus_tag="OO135_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5397654.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1096..2307
                     /gene="tyrP"
                     /locus_tag="OO135_001773"
     CDS             1096..2307
                     /gene="tyrP"
                     /locus_tag="OO135_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5397655.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2369..3034)
                     /gene="yecA"
                     /locus_tag="OO135_001774"
     CDS             complement(2369..3034)
                     /gene="yecA"
                     /locus_tag="OO135_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5397656.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3230..3316)
                     /locus_tag="OO135_001775"
     tRNA            complement(3230..3316)
                     /locus_tag="OO135_001775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3280..3282),aa:Leu,seq:taa)
     gene            complement(3329..3402)
                     /locus_tag="OO135_001776"
     tRNA            complement(3329..3402)
                     /locus_tag="OO135_001776"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3368..3370),aa:Cys,seq:gca)
     gene            complement(3457..3532)
                     /locus_tag="OO135_001777"
     tRNA            complement(3457..3532)
                     /locus_tag="OO135_001777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3497..3499),aa:Gly,seq:gcc)
     gene            complement(3684..4232)
                     /gene="pgsA"
                     /locus_tag="OO135_001778"
     CDS             complement(3684..4232)
                     /gene="pgsA"
                     /locus_tag="OO135_001778"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5397657.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4289..6121)
                     /gene="uvrC"
                     /locus_tag="OO135_001779"
     CDS             complement(4289..6121)
                     /gene="uvrC"
                     /locus_tag="OO135_001779"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5397658.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLLQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6118..6774)
                     /gene="uvrY"
                     /locus_tag="OO135_001780"
     CDS             complement(6118..6774)
                     /gene="uvrY"
                     /locus_tag="OO135_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5397659.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7069..7245
                     /locus_tag="OO135_001781"
     CDS             7069..7245
                     /locus_tag="OO135_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397660.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7232..7456
                     /locus_tag="OO135_001782"
     CDS             7232..7456
                     /locus_tag="OO135_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5397661.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7523..8245)
                     /gene="sdiA"
                     /locus_tag="OO135_001783"
     CDS             complement(7523..8245)
                     /gene="sdiA"
                     /locus_tag="OO135_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR5397662.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8475..9227)
                     /gene="yecC"
                     /locus_tag="OO135_001784"
     CDS             complement(8475..9227)
                     /gene="yecC"
                     /locus_tag="OO135_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5397663.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9224..9892)
                     /gene="tcyL"
                     /locus_tag="OO135_001785"
     CDS             complement(9224..9892)
                     /gene="tcyL"
                     /locus_tag="OO135_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5397664.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9907..10893)
                     /gene="dcyD"
                     /locus_tag="OO135_001786"
     CDS             complement(9907..10893)
                     /gene="dcyD"
                     /locus_tag="OO135_001786"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5397665.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10998..11798)
                     /gene="tcyJ"
                     /locus_tag="OO135_001787"
     CDS             complement(10998..11798)
                     /gene="tcyJ"
                     /locus_tag="OO135_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5397666.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(12369..13052)
                     /gene="fliA"
                     /locus_tag="OO135_001788"
                     /pseudo
     CDS             complement(12369..13052)
                     /gene="fliA"
                     /locus_tag="OO135_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(13372..15078)
                     /locus_tag="OO135_001789"
     CDS             complement(13372..15078)
                     /locus_tag="OO135_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5397667.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNALAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSVKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGTGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            15344..16750
                     /gene="fliD"
                     /locus_tag="OO135_001790"
     CDS             15344..16750
                     /gene="fliD"
                     /locus_tag="OO135_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5397668.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKNYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            16775..17185
                     /gene="fliS"
                     /locus_tag="OO135_001791"
     CDS             16775..17185
                     /gene="fliS"
                     /locus_tag="OO135_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5397669.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            17185..17551
                     /gene="fliT"
                     /locus_tag="OO135_001792"
                     /pseudo
     CDS             17185..17551
                     /gene="fliT"
                     /locus_tag="OO135_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            17629..19116
                     /gene="amyA"
                     /locus_tag="OO135_001793"
     CDS             17629..19116
                     /gene="amyA"
                     /locus_tag="OO135_001793"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5397670.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(19150..19563)
                     /locus_tag="OO135_001794"
     CDS             complement(19150..19563)
                     /locus_tag="OO135_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5397671.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            19750..20955
                     /gene="yedE"
                     /locus_tag="OO135_001795"
     CDS             19750..20955
                     /gene="yedE"
                     /locus_tag="OO135_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5397672.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            20952..21185
                     /gene="yedF"
                     /locus_tag="OO135_001796"
     CDS             20952..21185
                     /gene="yedF"
                     /locus_tag="OO135_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5397673.1"
                     /translation="MKNIVPDYRLYMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            21292..21963
                     /locus_tag="OO135_001797"
     CDS             21292..21963
                     /locus_tag="OO135_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR5397674.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARIETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(22003..23077)
                     /locus_tag="OO135_001798"
                     /pseudo
     CDS             complement(22003..23077)
                     /locus_tag="OO135_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5626 a         5947 c         5618 g         5886 t
ORIGIN      
        1 tagcggtaat gcgttttatt cccattcgta tttttaataa ttgaagttta tattttacct
       61 tttgcaaata ataaaataac aaattatagt gacgccacag caacaaatat aacctttgtg
      121 gagcactatc atgctgaaac cagaaatgat tgaaaaactt aatgagcaga tgaacctgga
      181 actgtactct tcactgcttt atcagcaaat gagcgcctgg tgcagctatc ataccttcga
      241 aggtgctgcc gcgttcctgc gccgtcacgc ccaggaagag atgacgcata tgcagcatct
      301 gtttgattac ctgactgata ccggcaattt accgcgtatt aataccgttg aatctccgtt
      361 tgctgaatat tcctcacttg atgaattatt ccaggaaacc tataaacacg aacaattaat
      421 cacccagaaa attaacgaac tggctcatgc tgcaatgacc aatcaggact acccaacatt
      481 taatttcctg caatggtatg tttctgagca gcatgaagaa gagaaactgt tcaaatcgat
      541 tattgataaa ttaagcctgg caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga
      601 actctctacc ctcgacacac aaaactaatg ctctccggcg gcaggtttac tgccgccgtt
      661 tcaaatcagt gacggcaaat cttactctga tcggtcgaaa aaccgtcttt cgaaggtata
      721 aatttgcctt ttgctgccag aaacgctacc agctctcccg ctgtcatccc ttctgccgag
      781 caggtgtgaa aacgtgcctg ttcaccaaaa cgcgctttaa ttgctgcttc cagactggca
      841 tgcgtatatt gctcgcctga ttcaatcatc atatttaaca cttcatgacc gtgaatagag
      901 tccatcgttc ctcctcaaaa aaagcctagc gtagcgattg ccgcttatga agactttgcg
      961 ccagagcaga actgaatgct ttttattgta catttatatt tacaccatat gtaacgtcgg
     1021 tttgacgaag cagccgttat gccttaacct gcgccgcaga tatcactcat aaagatcgtc
     1081 aggacagaag aaagcgtgaa aaacagaact ctgggaagtg tttttatcgt ggcgggaacc
     1141 acaattggcg caggcatgct ggcaatgccg ctggctgcgg caggtgttgg ttttagcgtc
     1201 acgttaatct tgttgattgg gctttgggcg ttgatgtgct acacggcgct attactgctg
     1261 gaggtgtacc agcatgttcc ggcagatacc ggtctgggca cgctggcaaa acgctatctg
     1321 ggacgctacg gtcaatggct gacgggcttc agtatgatgt tcttaatgta tgctctgact
     1381 gcggcataca tcagcggtgc cggtgaattg ttggcctcca gcatcagcga ctggacaggt
     1441 atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt ttgttgccgg tggcgtggtt
     1501 tgtgttggaa catcgctggt cgatttattt aaccgttttc tgttcagcgc caaaattatt
     1561 tttctggtgg tgatgctggt attgctgcta ccgcatattc acaaagtgaa tcttttaacc
     1621 ctgccgttgc aacaggggct ggctctgtca gcaatcccgg tgatttttac ctcatttggt
     1681 tttcacggta gcgtgccgag tattgtcagc tatatggatg gcaacgttcg taagctacgc
     1741 agggtgttta taaccggtag tgcgatcccc ctggtagcat atattttctg gcaggtggca
     1801 acgcttggca gcattgattc aacaaccttt atgggattgc tggctaatca tgctggatta
     1861 aacgggctgt tacaggcgtt acgcgaaatg gtggcctctc cgcatgttga gctggcagtg
     1921 catttatttg ctgatttagc cctcgccacg tcatttctcg gcgttgcgtt aggcttattt
     1981 gattatctgg ctgatttatt tcagcgttca aataccgttg gtggacggtt gcaaactggt
     2041 gcaattacct ttctgccgcc gttggcgttt gcactgtttt atccacgagg atttgtgatg
     2101 gcgctgggtt acgccggtgt ggcgctggcg gtactggcat tgattatccc ttcgctgttg
     2161 acctggcaaa gcagaaagca caatcctcag gcgggttacc gggtcaaagg tggtcgtccg
     2221 gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg gcgtgcaatt tttgattgcg
     2281 gcagggttgt taccagaagt ggggtgatca gatagcctca aattccttat tgggtgccag
     2341 aattaacgct gacacccaat ttggcctctt aatgcaggca gcactgctta aatttcttac
     2401 cactaccgca cgggcaagga tcgttacgcc ccggtttctc ttctgctttg atcggttgct
     2461 gaacagcttt ttcctgcgga tgcgccatcc agtacgcatg tagatcaagc gccgccagtc
     2521 gaatggcatc tacgctctct tcaaacgctt ctggcgacat cttttctacc cgctcgaagt
     2581 tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg ttttaacgaa tcgggcaacg
     2641 ttgaccagtc agaaagtgcc acgccccgca tatagccaaa gcaccactcc tcaacaatcg
     2701 tcagctcgct gccatcaact tctcgcaagc cgaataacgg ctcaaactgc tccgggaatt
     2761 cgttcagacg ctctgcggta tcggccatat gttgaaaagc caggttcata aagcgcgtca
     2821 tctctttctc tgacgcccag cgcggcacat agtcagcccc accccacacg gcaaccagcc
     2881 actgttccgg ttcaatctct tgcggagaac tcaacaccgc cgtcaataaa tcgtccagct
     2941 ccgccacatc aaggatggcg tggttagtgt tgtatttggt cagaatatcg tccagccatt
     3001 ccaactcact ttcgtttaac ggtcccgttt tcatacgctt ttccttgtgg atctcaactc
     3061 gccagcacct atcttacatg ccggtccgta tcagagatac tttttgagtg gctttgccgg
     3121 tgattaaaaa ttaaggagga tgtaacgaca agttgcaggc acaaaaaaac cacccgaagg
     3181 tggtttcacg acactgctta ttgctttgat tttattctta tctttcccat ggtacccgga
     3241 gcgggacttg aacccgcaca gcgcgaacgc cgagggattt taaatccctt gtgtctaccg
     3301 attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt ccggagtcga accggactag
     3361 acggatttgc aatccgctac ataaccgctt tgttaacgcg ccaaattctt caggcctttc
     3421 agccagacat ccgcttgacg ccgatgtctt ttaaactgga gcgggaaacg agactcgaac
     3481 tcgcgacccc gaccttggca aggtcgtgct ctaccaactg agctattccc gcattcatta
     3541 agcaatcagt taatcacttg attttattat cgtctggcaa tcagtgccgc ctttcgatgc
     3601 gttgcattct acttacctgg cgcgatgagt caacgatatt tttcaccact tttgatcgtt
     3661 tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat ctgcacgcgc agcgctcaaa
     3721 tattgcaaca ttgaccacag agtcagtacc gcagccacaa agaaaagtgc aataccggcg
     3781 tactcaaccc aaatgttcgg acgccacagc agccaggcca acgccaccat ctgggcagtg
     3841 gttttcactt tcccaatcca agagacagcc acgctactgc gtttacccaa ctccgccatc
     3901 cattcgcgta gcgcagaaat aataatttca cgggcgatca tcgttgccgc cggtagggtc
     3961 acccaccagc tgtgataatg ctcggttacc agcaccatgg cgatagccac gagaacttta
     4021 tctgctacag ggtcaaggaa agcaccaaac cgggtactct ggttccagcg gcgtgccaga
     4081 aaaccatcga accagtcagt caccgccgcg acgcagaaaa tgagcgcggc ggcaaacggc
     4141 gaccaggtga caggcagata aaagaccaat acaaagaatg ggataaggat gacacggaat
     4201 agtgtaagca acgtagggat attaaattgc ataatgacgg gtaactatct gttgtcagta
     4261 agattacccc tatgttgcta cagagacatc aatgtttcaa cgaccagaag atcttttctg
     4321 ccagaccttg cgaaataccc ggcacttttg caatttcctc gacgctggcg ttacgtaaac
     4381 cttgcaaacc gcccatatat ttcaacaaca tttgccgacg ttttggcccg acgccttcaa
     4441 tggtttccag ggaactggta tttttgacct tcgcccgttt tttacggtgc ccgccaatcg
     4501 cgtgatcatg tgattcatcg cgaatatgct ggataacatg cagcgcgggt gaatctggcg
     4561 gcaaactaaa tccctcacct tccggctcaa agaacagcgt ttccagtcca gccttacgat
     4621 ctgctccttt ggcaacgcca agtagcagcg gatgattttt atcccatgag acatccagtt
     4681 cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc gccgtcgata aggatcacat
     4741 ccgggatctt actgtcgtca atggctttac cataacgccg acgcagcacc tgattcatcg
     4801 ccgcataatc atcgcccggc gtgatgcctg taatgttata gcgtcgatac tccgcacgca
     4861 gcgggccgtt agcatcaaac accacacagg aagcgacggt ttgttcgccc atggtatggc
     4921 tgatgtcaaa gcactccatc cgcttcactt ccggcaattt caacacgctg gcaagcgcgg
     4981 tcagtcgctg gtgaacggta gattgctgca aaagtttgct ggttaaggcc gtcgccgcat
     5041 tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg aggtttggtt tgaacattaa
     5101 tcttgcgtcc cgccagttct gaaagggaat cggcgagcag cgttttatcg ctaagattaa
     5161 aatcgagcag gatctcaccc ggtaaggtgc gcatctggct gccttgtaaa tagaactgac
     5221 ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc aggcactttc gggaaatagc
     5281 tgcggctgcc gagcactttg ccctgacgaa tgaacaatac gtggacacaa gccatgcccg
     5341 catcgaacgc cacaccaata acgtcaaggt cgtcgccggt attggaaaca aactgttttt
     5401 cggtgacgcg tcgcaccgcc tgaatttggt cgcgaatacg tgccgcttct tcaaactcca
     5461 gattctgact ggcagtttcc atacggctaa tgagttgcgt aagcacctga tcatctttgc
     5521 cagacaaaaa caggcgcaca tactcgacct gctgagcgta ttcttcttca ctcaccagtc
     5581 cttcaacgca cggtcccaga cagcgcccta tctgatattg cagacacgga cgcgagcgat
     5641 tgcgataaac actattttcg cactggcgaa tggggaaaat cttttgcagt agcgccagag
     5701 tttcacgtac ggcatagcca ttcgggaacg ggccgaaata ttcacctttg gcatgcttcg
     5761 caccacgatg catcgccaga cgcgggtggg tatcgccact caggaagata aaaggataag
     5821 atttatcatc gcgtagcaaa acgttgtaac gcggctgata gagtttgatg tagttgtgtt
     5881 ccagcaacag cgcttcggtt tctgtatgag taaccgttac atcaatttgc tgaatctggg
     5941 cgaccagcgc ttcggttttg cgcgaagcga ggttgctacg gaaatagctg gaaagccgtt
     6001 ttttcaggtc tttcgctttg ccgacataga taaccgtacc accagcatcg tacatgcgat
     6061 aaacgcctgg ctggctggtt acggttttta aaaacgcttt tgcgtcaaac tgatcactca
     6121 ctgacttgat aatgtctccg cattacacag accatggcga attgccaggt gagtcagctc
     6181 aacatcgcca tgaatgttta gtttactgaa catacgatag cggtagctgt tcaccgtttt
     6241 cggactgaga ttgagctgtt ctgagatctc attgaccttc tggcccttgg tgatcatcag
     6301 cataatctgc aattcacgtt cagacaaact ggcaaatggg ctttctgttt tttctggttc
     6361 gatctggctt aacgccattt gttgagcgat gtcagaagca atgtaacgct gccctgaata
     6421 gacagaacga atcgcactca cgacttcctg cggagccgcg cctttgctga ggtagcccgc
     6481 agcaccggcc tgcatgactt tcgctggtaa agggttttct gtatgaacgg taagcatgat
     6541 gattttgaca tcagctgtgg aacgcgcgat tttacgcgtc gcctcaagac cgccaatgcc
     6601 cggcatactc atgtccatta gcaccacgtc aacggcattt gtccggcacc acttaacggc
     6661 gtcttcaccg cacgatgcct caccgacgac ttttataccc tttatatctt ccagaatgcg
     6721 tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt agaacgttga tcaaagaaat
     6781 atctccagaa atagggataa cgctactgat agttagtcat tcgtatatta gcggttttta
     6841 ttgcaacttt gaaacgttaa aaatggtggg tattcgattt ttctctcgtt tttgtcaatt
     6901 cgattgtcca cagttatggt cacgcccgtc accaacggct taccggatcg tttcacagta
     6961 agccctacga tgaaatgtga caaaaattga ctttattcag caaaaatgaa aatcagcccg
     7021 ctgaatatgt aacattaatt aacccgaaca atacgggcaa aaacattcat gataaaaata
     7081 tttattggtc attatattaa cgtattttat agcactgccg atatcacgct caaaaaacaa
     7141 ccactgctat ttttagcaaa gcttatggta tactccgccg ccttaacatt tttcaccgca
     7201 aattttcatt gcaacatgac gaggaaaata aatgagtacg cctgattttt ctactgccga
     7261 gaataatcaa gaactggcaa atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct
     7321 gcaggcgatg ggacaagctg acgcgggccg cgtgatgtta aagatggaaa aacagcttgc
     7381 gctgatcgaa gacgaaaccc aggctgcagt attttccaaa acggttaagc aaattaaaca
     7441 agcctaccgt cagtaatata aaaccggctg agagcgtgcc tttcagccgg tttttgcatc
     7501 tggcacgcag aaagaaaagc gatcaaatta agccagtagc ggccgcgtaa caggccacct
     7561 gggtcttatt tggtgcattg atttttttct gcatgttttt ctgatggaaa ttgaccgtat
     7621 tctcagagat tgacaaaatc atcgctatct ctgctgatgt cttcccttcc gccgtccatt
     7681 tcagaatttc tttttcgcgc ttgctgaaat tcatctctgg cgtcatcact atttcatcat
     7741 ttaaacgcat cagagccatc agactttcgc gcaccagtaa ctgcattttt aattgcagtt
     7801 catcactaag aatgggtatt tcgcgcgtgc tgcaacggga aaaggacaaa aagcccagcg
     7861 cccgatttgg cagcattaaa tactgagtga caccgcggcg taaaccatgt gcgtgcgcgg
     7921 cttcccataa tggctgtgct tcgctgaata agtcatcatt ccacattaaa tggccctgac
     7981 taaagttttc agggttcagc acaggatcaa ttgcgagaaa attttttgcc tgataataac
     8041 taacccacga ctcagggtaa ttggtgtaga aagccacttt aggtcgcgtg aatggtaccg
     8101 ggtggcgaac acataacgaa tagtaatcgt actccagctg ctgagcctga agctctattt
     8161 catggtagac ctcttctgcg gcctccatcc tctgaaaacg caacagcatc gtgcgacgcc
     8221 agctgaaaaa atccgtatcc tgcatagtaa accgcaacgc ccctgagagt gagtatcatt
     8281 tataatgata attcaaacta tcacataaat aagatttata tataatttat attattcagg
     8341 caatgaatta cttttgcaag ccatcgcatt ctcttatgtt attaatgagt tatgctgatt
     8401 tgttaagcag ttttatcacg cttgaaatgg cgtcccgccc cgacaggtga atcgtcgggg
     8461 ctgatttttt cttattattg cagcagaaac ttctcgagga actgtcgggt gcgaggctgc
     8521 tggggatcgg caaataacgc ttttgctgcc ccctgctcga ctatccgccc caggtccata
     8581 aaaatcgccc ggtccgcaac atcccgggca aagctcattt cgtgcgtcac aatcaccatc
     8641 gtgcgctttt cctgcgccag ctggcggatt gtgttcagga cttcgcccac cagctctgga
     8701 tccagcgctg acgttggctc gtcaaacaaa atcacctcag gacgcattgc cagcgcacgc
     8761 gcaatcgcta cacgctgctg ttgaccgcca gacaaacgac gtggatagct ggtttcttta
     8821 ccggccagcc caactttcgc cagcagctcg cgagcgcgcg ccgtggcctc ttctttcggt
     8881 tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg atgcggaaac
     8941 aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat cagcgatttt
     9001 tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat cgttcccgct
     9061 tccggttgtt ccagcagatt tatgctgcgt agcaacgtgg ttttgccgga accactcgga
     9121 ccgataattg ccaccacttc gccaggcttt acctcgaggt cgataccgtg cagcaccgtc
     9181 tgaccgtgga attttttcac caggttctta acttcaatgg cactcatttg ggttctctct
     9241 cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataacaca gtcgccatga
     9301 tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg cgcgaggtaa
     9361 tcaactgcgc ctgacggaac agctccggca cctggattgt cgcggccagc gacgtatctt
     9421 ttaccagact gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc gcctgtggca
     9481 aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagcg gcttcccact
     9541 gacctttatc aatagaagaa atggcggcgc gcagcgtttc ggcggcatag gcggcagtgt
     9601 tcagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca aactgcggca
     9661 ggccgtaata gatcataaac agttgtgcga tgagcggcgt accacgaaaa atagagatat
     9721 aaaaacgcgc cagccagcgc acgggccaga ttggcgacag gcgcatcagc gcgagaataa
     9781 acccgagtag taagccaaaa aacatgccgc cgatactgag ttgcagggta tacccagccc
     9841 ctttcaacag gaacggcaag gaatcaataa ccagttgtat actttcttgc atgagcgttc
     9901 ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg tatgaataaa
     9961 cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca gccccgccat
    10021 cgcttttccg gtatacacag gatcaagcag aatgccttcc agacgcgcca gcaatttcac
    10081 tgcttccatg ccttcgtcgt tcggcacgcc gtagccaggt gcaaaatagt catcccagag
    10141 taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt gtaggttaac
    10201 cactttcggc aattgatcgg caacggaacg cgacacggtc acgccaatca gttcgctttc
    10261 aggcatcagg tgttccagcc caacagccaa tccggcgtga gttccggcac tgcccgatgc
    10321 gaccaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt ccagcgcact
    10381 ctccacataa cccagcgcgc ccagagcatt agaaccgcca accggaatga catacgggcg
    10441 aaagccttgt gcttcgactc gcgtcgccag ctcttccagt tgggcattgg gatcggtcag
    10501 tgcgtcgcac atttcaatct gggtattgaa cagattcagc aacaaacgat tgccgttggt
    10561 taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc agtgcaggcc
    10621 gagtttcgcc gcaactgcgg cagtctgccg cacatggtta gactggatcg cgccggcagt
    10681 aatcagcgta tcggcacctt cgcgcagggc atctgccgcg agaaattcca gcttacgtaa
    10741 tttattgccg cccattgcca tgggtgtgac gtcatcccgt ttgatgaaaa tttcccgtcc
    10801 aagataatca gaaaagcgcg gcagatattc gagcggcgtt ggcgcgccga taaactccag
    10861 ccgtggaaaa cgggttaaat tatgcagtgg cataatagcc tccgatgtgt gttgttgtga
    10921 ttttcttatt atgcacgctg aaaacgcgtt aataaaaaag gcgctagtga aagcgccctt
    10981 ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc ggaaagggct
    11041 tgcagagtgc catctttttg catttccgca attgcatcgt tcactgcttt cagcaggtct
    11101 tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc accggttact
    11161 gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacggtc aacgaggatc
    11221 gcatcgatac ggcctacgcg cagatcctga tatttggtcg ggtcatcatc ataggtacgc
    11281 acatcgacgc cctgaacatt ctgccgcagc cactcttcat agttggtacc cagaccgaca
    11341 cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt accttttttc
    11401 accagcgcct gaataccaga aatggtgtac ggggtagaga aatcgtattt tttcttgcgc
    11461 tcatcagaaa tggtgacctg attaatcacc acatcaatac gtttagagtc cagcgacgct
    11521 agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaagatg ttttgccagc
    11581 tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg aaaactgaac
    11641 ggcggataag ttccttccag ccctaccagt agcgtaccgc gctctttgac tttattaagc
    11701 agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgccac ggccatcaca
    11761 cccatcaatg cctgacgtcc cagatgtgct aatttcatat tcaccccgaa tgttgttatg
    11821 tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct acatcttatt
    11881 cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag tccggtacac
    11941 gtttgacgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt ggtgctggtg
    12001 gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagctg atcgagggaa
    12061 atattttgct catgtagatt attccccaaa cggcgcaaac gaacgacata ttcacgcacc
    12121 gtacctgggc tcatttcagt ttgctcaaaa agaaattgct taaagccgat aatatcgaaa
    12181 aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc cgcccatgac
    12241 tgttgttcca cttgccagcc attttcatca agcagcgtgt ccaggcggga aatctcgatt
    12301 ttattcacta ttttgccgtc gcgaactaag gtaatgcgat cgagtaattt acggcaatgc
    12361 gcgcaatggg cctggctgtg taactgactg acccgcgatt ccccgacctc cagcaccgcg
    12421 ccaatctctt tgagattcag ctcttcctgg taatagaggg ttaataccag tttttcgcgc
    12481 tccggcaacg tttcgatagc ttccatcacc cgctggcgca gattactgtc cagcagttgt
    12541 tgtagcgggt tttctcgctg atgatcatca gtaaccagtt cgatgctatc gccgtgctct
    12601 tcgcgccact catcgtagga gaaaagctgg ctgttattgg tgtcgagcaa catttggcga
    12661 taatcggcaa tatcgatccc taaacgttcc gctacctcag tttccgtggc gttgcggcca
    12721 agttcctgct ccagttgccc tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg
    12781 cgcggcaccc agtcacggct gcgaagttca tccagcatag cgccacggat acgctgcact
    12841 gcgtaagttg taaatgccgt tccttgtagg gcgtcatagc gttcgacggc attaagtaac
    12901 ccaatgccgc ccgcctgtag cagatcgtca agttccacgc tcgcgggcag tcgaacctgc
    12961 aggcgcaatg cttcgtgacg caccagcggg acataacgct gccacagaga gtgtttatcc
    13021 attacacctt cagcggtata gagtgaattc acgataaaca gccctgcgtt atatgagtta
    13081 tcggcatgat tatccgtttc tgcagggttt ttaatcggac gattagtggg tgaaatgagg
    13141 ggttatttgg gggttaccgg taaattccat gcagaaaaaa ccccgccggt ggcggggaag
    13201 cacgttgctg acaaattgcg ctttatgttg ccggatgcgg cgtaaacgcc ttatccggcc
    13261 tacaaaaatg tgcaaattca ataaattgca attcaccttg taggcctgat aagtgcagcg
    13321 catcaggcaa tttggcgttg ccgtcagtct cagttaatca ggttacgacg attaaccctg
    13381 cagcagagac agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc
    13441 ctgctggatg atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg
    13501 aatacgggac tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcaga
    13561 atccagacgg ttctgaatag cacccaggga tgaacggaat ttgtcgatgg agctgatagc
    13621 ttcatccagg gcagccagag gatctttggt tgcagtgcct gcactggtgg tttcagtggt
    13681 cagtttgcct gtagagttaa cataagcctg tgccccaaca gtagctgcaa atttaccagt
    13741 tgcacctgtg ccatttgaag ctacagttac tgtaccggta tccttatcga cgttgtagtt
    13801 gatagtgtaa gatgcggttg tggtgagctc tttgtctgca tcagtatagg tgtcattcga
    13861 attaccagca ccattagtat tcgcaccacc gaaggacgcc gttgcagact gaatacctgt
    13921 attatactta attgtcgcac cagtaactgc tgtgttagcc gttgcagcac tctgcactgt
    13981 gctcagtaca gtatcagcgc ttacagtttt ggtgtaggtt acgccagaag ttgccgctgc
    14041 accaaagtta taagttgtgc cagacaactg gatagaacca gttgtcgcta aggtactacc
    14101 gctagcaaac agatcggaca atttcactga agatgcaatg ccggcgttgt ttgtagatag
    14161 gttaccagtg gcgtccaggt acgcggcttt accaccgatg gtcacatcgc cattagcatc
    14221 tacatcaaac ttcacatcac cagtactacg agtataaaca cctgatgcag tagtgccagc
    14281 cgctggtttc agagtattcg caaagttaac aacatcgcca tctgcaactg ttgcttgagt
    14341 ggtgaagttc cctttagccg catcataagt atagatcgca gcactcgagc cagtagtagt
    14401 aactgtatct ccggttttca ggcgagacag tgcatcgcta gcgctgagtg ctgtattgtt
    14461 tgtggtcaca gcataaggac ctgttcccgt tgcaccagca gcggtcagat cgctgactgt
    14521 agcagctttg ttcgcaatag tgcctttgcc attcacgtta aaaccattca gccccagcgt
    14581 atctgagtca attttcttca gatcaatcgt gatagtctgg ccgtcattcg caccaacctg
    14641 aattttcatt gaaccgtctt tcgccagtgc gttcacgccg ttgaactggg tctggccaga
    14701 tacgcggtca atttcgtcca gacgggattt gatttcgtcc tgaatggagt ccagatccga
    14761 atcggagtta gtcccggtag aagcctgaac cgtcagttca cgaatacgct gtaagttgtt
    14821 gttgatttcg gacagcgcgc cttcagtggt ctgcgcaacg gagataccgt cgttggcgtt
    14881 acgggccgcc tgagtcaggc ctttaatatt agaagtaaaa cggttagcaa tcgcctgacc
    14941 tgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct cgatagaact
    15001 cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt
    15061 attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgtattggg
    15121 tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt
    15181 agaatttttt tcacaaacag ccattttttg ttagtcgccg aaatactctt ttctctgccc
    15241 cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa
    15301 ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc
    15361 gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca ccgccgcgca
    15421 aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc
    15481 ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg cattgtctaa
    15541 agccgatctt ttttccgcca ccagcactac cagcagcacc accgcgttca gtgccaccac
    15601 tgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg cgcaggcgca
    15661 aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca ccagcgacag
    15721 caaactcacc attcaacaag gcggcgacaa agatccgatt accattgata tcagcgcggc
    15781 taactcatcg ttaagcggga tccgtgatgc tatcaacaac gcaaaagcgg gcgtaagtgc
    15841 gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa atgacaccgg
    15901 ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa gttttatggg
    15961 ctatgacgcc agtgccagca gcaacggtat ggaggtctcg gtcgccgccc agaatgcgca
    16021 gctgacggtc aacaacgtcg ccattgagaa cagcagcaac accatcagcg acgcgctgga
    16081 aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa ccatcactca
    16141 ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata actcgttaat
    16201 agataccttc agtagcctga ccaaatacac cgccgtagat gcgggagctg atagccagag
    16261 ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga cgcagttgaa
    16321 gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc agattggtat
    16381 cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca ccgctgcact
    16441 gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa aaaccggcat
    16501 cacgaccacc atcggcagca acctgaccag ttggctttcg acaacgggca ttattaaagc
    16561 cgctaccgat ggcgttagta agaccctgaa taagttaact aaaaactaca acgccgccag
    16621 cgatcgcatt gatgcgcagg tcgcgcgcta caaaaaacaa tttacccaac tggacgtttt
    16681 aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa acaacagtaa
    16741 ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc
    16801 ctatgcacaa attggcgtcg aaagcgccgt aatgagcgcc agccagcagc agctggtcac
    16861 catgctattt gatggagtgc tgagcgcact ggttagagcg cgcctgttta tgcaggacaa
    16921 caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact
    16981 gcgggtgagt cttgatgaag agagcaaaga cgaactaacc caaaacttga ttgctcttta
    17041 tagctatatg gtcaggcgct tgctgcaagc caatttacgc aacgatgtct ccgcagtcga
    17101 agaagtggaa gcattaatgc gcaatattgc cgatgcctgg aaagagtcgt tactctcccc
    17161 ttctttgatt caggacccag tctgatgaac catgcaccgc atttgtattt tgcctggcaa
    17221 caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggacgaa
    17281 ctcatcgcca gcgaaatggc gtatgtgaat gcggtgcagg agtatagcac acctgactga
    17341 agaggttgcc ccgtccacca cgatgcagga gcagctccgc ccgatgctgc gcctgattct
    17401 cgacaacgaa agcaaggtaa agcagttatt acagattcgt atggatgaac tggcgaaact
    17461 ggtcggtcag tcatcggtgc aaaaatcggt gttaagtgcc tatggcgatc agggcggctt
    17521 tgtgctggct ccgcaagata acttctcgtg aatcttaacg agctaatggt tcgcgaataa
    17581 tccgattacg gctacgcttc taatgttccc cttgaatgga gtcgaagaat gcgtaatccc
    17641 acgctgttac aatgttttca ctggtattac ccggaaggcg gtaagctctg gcctgaactg
    17701 gccgagcgcg ccgacggttt taatgatatt ggtatcaata tggtctggtt gccgcccgcc
    17761 tataaaggcg catcgggcgg gtattcggtc ggctacgact cctatgattt atttgattta
    17821 ggcgagtttg atcagaaagg cagcatccct actaaatatg gcgataaagt acaattgctg
    17881 gccgccattg acgccctgaa acgtaatgac attgcggtgc tgttggacgt ggtagtcaac
    17941 cacaaaatgg gcgcggatga aaaagaagct attcgcgtgc agcgtgtaaa tgctgacgac
    18001 cgtacgcaaa ttgatgaaga aatcattgag tgcgaaggct ggacgcgtta caccttcccc
    18061 gcccgtgccg ggcaatactc acagtttatc tgggatttca aatgttttag cggtatcgac
    18121 catatcgaaa accctgatga agatggcata tttaaaattg ttaacgacta caccggcgaa
    18181 ggctggaacg atcaggttga tgatgaatta ggtaatttcg attatctgat gggcgagaat
    18241 atcgattttc gcaatcatgc cgtgacggaa gagattaaat actgggcgcg ctgggtgatg
    18301 gaacaaacgc aatgcgacgg ttttcgtctt gatgcggtca aacatattcc ggcgtggttt
    18361 tataaagagt ggatcgaaca cgtacaggaa gttgcgccaa agccgctgtt tattgtggcg
    18421 gagtactggt cgcatgaagt cgataaactg caaacgtata ttgatcaggt ggaaggcaaa
    18481 accatgctgt tcgatgcgcc gctgcagatg aaattccatg aagcatcgcg catggggcgc
    18541 gactacgaca tgacgcagat tttcacgggt acattagtgg aagccgatcc tttccacgcc
    18601 gtgacgctcg ttgccaatca cgacacccaa ccgttgcaag ccctcgaagc gccggtcgaa
    18661 ccgtggttta aaccgctggc gtatgcctta attttgttgc gggaaaatgg cgttccttcg
    18721 gtattctatc cggacctcta cggtgcgcat tacgaagatg tcggtggtga cgggcaaacc
    18781 tatccgatag atatgccaat aatcgaacag cttgatgagt taattctcgc ccgtcagcgt
    18841 ttcgcccacg gtgtacagac gttatttttc gaccatccga actgcattgc ctttagccgc
    18901 agtggcaccg acgaatatcc cggctgcgtg gtggtcatgt cgaacgggga tgatggcgaa
    18961 aaaaccattc atctgggaga gaattacggc aataaaacct ggcgtgattt tctggggaac
    19021 cggcaagaga gcgtaataac cgacgaacac ggcgaagcaa ccttcttttg caacggcggc
    19081 agcgtcagcg tgtgggttat cgaagaggtg atttaaattc atccccggcg gcaagccggg
    19141 gagatttcat tacggcagtg gcgtcggtaa cggcgcttta tccagcgcgg cggcacactc
    19201 tgccgtcggg cgatccacac gctccatcgt cataccatcg tactcaatgg tgttaccgtc
    19261 acgctcaacc tcgtacagct cacgttttac ggtaacgtta gtgagatcat cagacatcaa
    19321 tgtcaactta cccggcacag caatcacccg ctgccactga cggcaatcca gagtatcacc
    19381 ctctttagtg acaatcaggc tgcctatcgc ttccggactg accagcgcac gttgcggccc
    19441 tttggtctgc cagtagcccg ccagccagtc cggcgcgggg gttttcacga cattgttata
    19501 gttttcgact tcggcacagc ccgccagcaa catgagcgcg cctgcaattg cgagtttttt
    19561 catcattcgt cctgcatgag aagaaaagag gattgtggca ttaaagccct gatgtcgcca
    19621 gcctttccag gttcagagag aaatttgatc taactattat ttttgtgaat tattaccccg
    19681 aaaataaatt tgcatgatgt atgattcgcg actcttgttt caaaccttct gagttcagag
    19741 gctacattca tgtcatggca gcaattcaaa cacgcctggt tgattaaatt ctgggcaccc
    19801 atccctgcgg tcatcgcggc gggtattctc tccacttact attttggcat tactggcacc
    19861 ttttgggctg tcacgggtga atttacccgt tggggcggcc agctcctgca gctgtttggc
    19921 gtccatgctg aagagtgggg ttattttaaa attatccatc tggaaggatc gccattaacc
    19981 cgcatcgacg ggatgatgat cctcggcatg tttggcggct gctttgccgc agcgctgtgg
    20041 gccaacaatg tcaaactgcg catgccacgc agccgtatcc gcattatgca ggccattatt
    20101 ggcggtatta tcgctggttt tggcgcgcgt ctggcaatgg gctgtaacct ggcggcgttc
    20161 tttaccggta ttcctcagtt ctcgctgcat gcctggttct ttgccatcgc cactgccatt
    20221 ggttcatggt ttggcgcacg ctttaccctg ctgcccatct tccgtattcc cgtgaaaatg
    20281 cagaaagttt ctgccgcctc accgctgacg caaaaaccgg atcaggcgcg gcgtcgtttt
    20341 cgtctcggga tgctggtctt tttcggtatg ctgggctggg cgctgctcac ggcgatgaac
    20401 caaccaaaac tggggctggc aatgctgttt ggcgtcggct ttggtttact gattgaacgt
    20461 gcgcaaatct gctttacttc ggcgttccgc gatatgtgga tcaccggacg tacccatatg
    20521 gcgaaagcaa tcattatcgg tatggcggtg agtgccatcg ggatcttcag ttacgtacag
    20581 ttaggcgttg aacccaaaat catgtgggcg ggaccaaacg cggtaattgg tggtttactg
    20641 tttggttttg gcatcgtgct ggcaggcggc tgcgaaaccg gctggatgta ccgcgcggta
    20701 gaaggccagg tgcactactg gtgggtcggt ctgggcaatg tgatcggctc gacgattctg
    20761 gcgtattact gggatgattt cgctccggcg ctggccaccg actgggacaa aatcaacctg
    20821 ctgaaaacct ttggcccgat gggtggcctg ctggtgacat atttgctgtt gtttactgcg
    20881 ctgatgttga ttatcggctg ggaaaaacgc ttcttccgcc gtgcggcacc gcagactgct
    20941 aaggagatcg catgaaaaat atcgttcctg attaccgcct ttatatggtg ggtgaaccct
    21001 gcccttatcc ggcagtcgca acccttgagg cgatgccgca gttaaaaaaa ggggaaatcc
    21061 tggaagtggt gagcgactgt ccacagtcga tcaataatat tccactggat gcgcgtaatc
    21121 acggctatac ggtgcgggat attcagcaag acgggccgac cattcgttat ttaattcaga
    21181 agtaatccct cattcccgct gtggcccaca gcgggaacat cgttgcctct gacacataga
    21241 ttttacccgc cccctctcct attcttttca gtatgatctc aggaggtttc tatgtgtgga
    21301 cgctttgccc aatcccaaac gcgtgaagat taccttgcgc ttctcgcgga agatattgaa
    21361 cgcgatattc cctacgatcc cgaacccatt ggcagataca acgtcgcgcc gggaaccaaa
    21421 gttcttctgc tcagtgaacg tgatgaacac cttcatctgg atccggtttt ctggggatat
    21481 gctcccggat ggtgggataa accgccgctg attaacgccc gcatagaaac tgccgcaacc
    21541 agtcgcatgt ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc cgatggctgg
    21601 tttgagtgga aaaaagaagg cgacaaaaaa cagccttttt ttatctatcg tgctgatgga
    21661 caacctattt ttatggccgc gataggtagc acaccatttg agcatggtga cgaagccgaa
    21721 ggatttttaa ttgtcactgc tgcagcagac cagggtctgg tagacattca tgaccgccgc
    21781 ccgctggttc tgtcgccaga agctgcacag gaatggatgc ggcaagagat tggcggtaaa
    21841 gaagcatcag aaattgctac caacggctgt gtgccagcaa accagttcac ctggcacccg
    21901 gtatcgcgcg cggtaggtaa tgtcaaaaac cagggcgcgg agttaattca acctgtttgt
    21961 tgacatcctc cacgccctga aggacgaggc tccctgctac gttcaggctg tcgcctgaat
    22021 catttcggtg ggttcctgct tcaacgggcg gcctgactgc accatctctc cacaggcaag
    22081 aacggcgtgc cccgccgcta aaatgttacg agcgccattt acatcggcgt tcgctgtata
    22141 tccacatacc tggcatctga atttactttg tgacaggcgg ttttctttcg ctgtatgacc
    22201 acagcacgcg caacgctggc ttgtgtacgc tggcggaaca gcaagcacct gaccgccacg
    22261 ccagagctgc ttgtactcaa gctggcggcg catttcatac cagccctgat ccagtatcga
    22321 acggtttaaa cctgattttg cccggacatt gcgccccggc tggctgaccg tacccgctgc
    22381 tgactttgac atgttgctga ccttcaaatc ctcaatgact atcattgcgt ggtttttgct
    22441 gacggtcgtt gtgactttgt gaaggtagtc cctgcggata tttgcgatac aggaatgcag
    22501 tcgctgtatt ttgcgtttct gcttctgcca gttgttgctg aatttgacct tgcggcttaa
    22561 ctgtcgctga agtctcgcca gcttcttctg gtttttctga aaactgttta caggctcaaa
    22621 ggctgtgcca tctgacagcg tggcgagttt agccacgcca gcatccagcc cgaccattga
    22681 tgctgaaggg tgaaccggag ttgatacttc actttctgtc tgaatactga tgtaccactt
    22741 accgcaggac tggctgacag tgacattttt cacaacaccc gtgacctgac ggctgttccg
    22801 gtagcgcatc cagcccagtt tcggcagaaa aatacggctg ttttcctgat cgagcttaac
    22861 gccctgcggg tagcggaata catcattctg tccccgcttt ttgaatcggg gaaaagcagc
    22921 ccgattctgg aagaagtttt tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg
    22981 ctgtgacggg gcatctttaa gccattacgt ttcagtggca tttttccact caaccagcca
    23041 ggaagccatt ttcccgtaag ggatgtattt attaccg
//
LOCUS       DAJTMK010000055        22974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTMK010000055 DAJTMK010000000
VERSION     DAJTMK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22974
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.056"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1266)
                     /gene="wcaD"
                     /locus_tag="OO135_001799"
     CDS             complement(<1..1266)
                     /gene="wcaD"
                     /locus_tag="OO135_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5397675.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKAGNDSNLLIVFYVQIMPDDLVMQ"
     gene            complement(1241..2458)
                     /gene="wcaC"
                     /locus_tag="OO135_001800"
     CDS             complement(1241..2458)
                     /gene="wcaC"
                     /locus_tag="OO135_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5397676.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLINYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKYSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2455..2943)
                     /gene="wcaB"
                     /locus_tag="OO135_001801"
     CDS             complement(2455..2943)
                     /gene="wcaB"
                     /locus_tag="OO135_001801"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5397677.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKSVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2946..3785)
                     /gene="wcaA"
                     /locus_tag="OO135_001802"
     CDS             complement(2946..3785)
                     /gene="wcaA"
                     /locus_tag="OO135_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5397678.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKVCLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(3878..6040)
                     /gene="wzc"
                     /locus_tag="OO135_001803"
     CDS             complement(3878..6040)
                     /gene="wzc"
                     /locus_tag="OO135_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5397679.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNMFPIFGAGWDRLMGRQNEAVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLQEKRQALEEEKAKLNGRVTAMPKTQQEIVRLIRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(6043..6486)
                     /gene="wzb"
                     /locus_tag="OO135_001804"
     CDS             complement(6043..6486)
                     /gene="wzb"
                     /locus_tag="OO135_001804"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5397680.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6492..7631)
                     /gene="wza"
                     /locus_tag="OO135_001805"
                     /pseudo
     CDS             complement(6492..7631)
                     /gene="wza"
                     /locus_tag="OO135_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
     gene            7940..8089
                     /locus_tag="OO135_001806"
     CDS             7940..8089
                     /locus_tag="OO135_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397681.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            8290..9873
                     /locus_tag="OO135_001807"
     CDS             8290..9873
                     /locus_tag="OO135_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5397682.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(10147..12000)
                     /gene="asmA"
                     /locus_tag="OO135_001808"
     CDS             complement(10147..12000)
                     /gene="asmA"
                     /locus_tag="OO135_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5397683.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVDGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(12022..12603)
                     /gene="dcd"
                     /locus_tag="OO135_001809"
     CDS             complement(12022..12603)
                     /gene="dcd"
                     /locus_tag="OO135_001809"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5397684.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12695..13336)
                     /gene="udk"
                     /locus_tag="OO135_001810"
     CDS             complement(12695..13336)
                     /gene="udk"
                     /locus_tag="OO135_001810"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5397685.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13654..16964
                     /locus_tag="OO135_001811"
                     /pseudo
     CDS             13654..16964
                     /locus_tag="OO135_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(17073..17921)
                     /gene="alkA"
                     /locus_tag="OO135_001812"
     CDS             complement(17073..17921)
                     /gene="alkA"
                     /locus_tag="OO135_001812"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5397686.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            18055..19407
                     /gene="yegD"
                     /locus_tag="OO135_001813"
     CDS             18055..19407
                     /gene="yegD"
                     /locus_tag="OO135_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5397687.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANCRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(19420..21360)
                     /gene="yegI"
                     /locus_tag="OO135_001814"
     CDS             complement(19420..21360)
                     /gene="yegI"
                     /locus_tag="OO135_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR5397688.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPVSKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVTGFMMPKVSGKEPIHMIYS
                     PGHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLAMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYSAHLTDCLWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(21357..22118)
                     /locus_tag="OO135_001815"
     CDS             complement(21357..22118)
                     /locus_tag="OO135_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HCR5397689.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTELAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFIGLAAATQE
                     QLDLLPKLLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(22115..22774)
                     /locus_tag="OO135_001816"
     CDS             complement(22115..22774)
                     /locus_tag="OO135_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5397690.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         5484 a         6077 c         5814 g         5599 t
ORIGIN      
        1 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc
       61 attacccgct tttcgcaaaa taaacggaca gacgattaaa aatgcatatt ccgggctaaa
      121 tatcgaaccg gtaaaaaaca gcgataccgg agtaaaaaga tataattgca cgcgaaaatt
      181 gcggttatca ccaaaagcgt tgatcatcat ttttatcact ttccccatgt accacagcga
      241 taaaaacacc gcgaaccagg aaaaataaat aatcagcaga tacaaaccat tgtctatggt
      301 ttttccgaca tccgcaccgt taaatattcc gaatgatgcg acatattcat aaagtgagcc
      361 aaatctgact acaccgtcaa tatgggtcaa agaataaccg accatcacca gcggaccgac
      421 aatacgataa tacgatgacg atccttccgt acctaaatcg cccagacggg tggaaatata
      481 aggaaacgcg attaccacac caaccaggaa tactgccaga gatatcaatg ccaacggtaa
      541 ctttttctta atcgcctctt tattcagata ttgaaatgcc cactccagca aataaaacag
      601 gataaaggtc ataacccctg aaaacgatcc ggataatatt atccctgcga gaatcatagc
      661 atcggtttta ggcgttttga taccaaactg tttgatgctg agccaaattg agattaatgc
      721 cagagcgaaa aatgccggtt cgaaataaag tgctgtcgtg cgcttgccgc cgaatttaat
      781 gaaattcagt acatagctgt tgctgtaaat cagatatttc gaaatcgact ccataatact
      841 gctgccgccg gtgaggataa tttgcgccat ctccaccgcc gccagcgcca ccaccagccc
      901 gaccaccaga taaaagaaac gtaatatctt gcgatggttg tgtggcgaaa ttgttttaaa
      961 acgaatactc cacaccatgc caataatgat cacaatatag acaaacagca tagttgaagt
     1021 gacgtattta ctggcatcca gcgactgacc aaacagatag ttaaacgccg tgagtcccgc
     1081 gccaatccct aaggcaatca tcaatttttt aacgctgatg cgttctaaaa acagcagtaa
     1141 caagacaggt aaaaaagtga cgatggtgat ggggaaactt tcgccaagct gggcgatttt
     1201 gacgttaacc agcagataaa tcagcggcag caacaggtag ctacagattc tgatagaagt
     1261 tgacatactc ctccagcatc tgttgtccac tgtaggcggc gcggctgcgt tggctgaact
     1321 cagccagcgt ggtaccaaat atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt
     1381 gcagcacctc ttcttcgctg acggttttac cgccggattt ttgcaacact tcccgcgccg
     1441 catcgctatg ggtggcaatc accggcacgc caatcgatag cgcctcacac aaaatcagcg
     1501 ggtagttatc gacgcgagaa ctgaacacca gcgcatccat ctgattgagc gcgctcatca
     1561 acttgcgctt gtcagtttca aagccgtgat taaccacgtt gccagcggtg aacggcgagt
     1621 acttaccaaa ggtatgtagt tcaattttgt cgcccagcgc catcatctcg cgtaccagtt
     1681 gctggttagt tttgccgtcg taacgcagat catgcgccac caccgcgatt ttcggcttgc
     1741 cctgggtttc gcgcaccgga ggcaagtccg ccagaatcgc ttcggttgcc atatcaatgc
     1801 cattattgat aatccggcaa cgccctggac cgtacaggct attgaaagcg tcagccacat
     1861 gctggctggg ggaaataaac tgacagccca gcgccagcat ctcacggaat aactggcgtt
     1921 tgcccgccac cagctggtgt gcgcgatcaa tcttcaccgg cggataatta attaaggtcg
     1981 ggcatttctg gcagcccgtt ttccagcctt cacaaccgtc ggtaaaggcg cagcgcccgg
     2041 taacgctcca gtggtcgtgc agcgtccaga ccagagtgac atccggtttg tggtttttca
     2101 ccttttcgca aaagcgcacc acgctcttaa gatttagcca gtagctgtgc agcacatgaa
     2161 aatgcaggac caccggaccc ggtgtacgag taatggtgcg atataactca ttgaaattgc
     2221 caaacagatc gcgattaaac agacgaaaca gggcaatgtt cgccatcgcg gtcatccgcg
     2281 gcgtatgttt gatgacctgc ggatagttct gatggctgac gctctctttg ccgcctttgc
     2341 cgtagccgta gacaaaatgt gacgccagcc cctgttgcag cgcacgctgg tggagatcta
     2401 acgccacacc tgctgccccg ccttccgcca gtcgcacatt aaattgcaga atattcattt
     2461 gattaccttc actcgcgctt tttctcccac caccagcgcg ttgtccggga cagagtcaag
     2521 caccacgctg cccgcgccca cggtgacgtt gttaccaagc gtgatatcac caagaataat
     2581 gacgttggca ccgagttcga cgccgttgcc aatgtgtgga catgccatgt tatcggcacc
     2641 acgattgccg atagtgacgc cgtggcgaat ggtgaaatca tcccccgcca ccacgttttt
     2701 attgatcact acggcgtaac cgtgatggat agtaaagcgg cggccaatgg tcgcggcagc
     2761 ctggatttca taaccgaaaa agcattcggt gataatgcga tacagcacca gcagcggggc
     2821 cgcccacaga ttgttgagga cgctcttttt gcgccacacc gaacaaaaat gagcgacgcg
     2881 ataggcaaga accatgcagc acgggcgtaa actccagctg ttggcgcgca gatcttccag
     2941 catctttagc gcccccgaat accatcagcc agacgtttgc cgttacgcac cgacagcagc
     3001 gtcagcaaag tgcgccaggt catgcgttta ttgcggatct ggtagagggt aaacagctga
     3061 tattttttgc tggcgcggtc aaatttgtct ttgtgcttac ggtaaaagtg gaagtatccg
     3121 gagaattttt tcggtgacga ggtgatctgc atttcgccgt gattgatatg caggatctgc
     3181 gtcgcctctt ccactttcca cggttcgccg tactccacca ccatccgcag gaaaatgtcg
     3241 taatcctgtg ccgctttcag ttcggtatcg aataagcaca ctttgaaacg ccacgcccag
     3301 gtaaaaacct gattgccaat gatattgcgt ttgtagaaca ggcgacgtga atacggcgat
     3361 ttgggataca gtggtaagct tgccggttgg gaataaactt ctccctggca tacgtagtcg
     3421 ttagcgtaca aaaaggcgtg agtgaccagt tgctgtttat gggcgaggaa gacgctcaga
     3481 cggttgggtg tccattcgtc atcgtcatcg atcccggtaa tgtattcccc ttgcgccagc
     3541 ataatcgcct ggttacgtac cgcgcacgcc ccgctgttaa tgtcgttgtg aatgtaggtg
     3601 atacgcggat cgttgagggc agtgacgtac tgttgcagtt gttcccagga ggtggagcaa
     3661 tcatccacga tgatcatctc ccagttgctg tagtcctggc gcaggaccga ttttatcgcc
     3721 cgaatcgcca gttgttggcg gttccaggtc ggcatataga ttgaaatcag cggattgtct
     3781 ttcataggtt gttctcccgg cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc
     3841 ttatccggcc tacggggcgg tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt
     3901 cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg gagttcagaa
     3961 tcaccccttt caccggaata ccgttttgct caaagcggct cagactggtt tccacttctt
     4021 tcaatgtgtt gaccgcatag cgcgccacca ttaacgtggt tccagcgtga cgcccgacaa
     4081 ttgctgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg tcgtagtttt
     4141 tactcgccca gctcaccaat tcggcaaagc gttcgctcat caacagttca gaaggatttg
     4201 gcggcacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt ttagcagcgg
     4261 tagtaatatc gccctgacca atcagaattt ccgacaggcc attaacgtta ttggtgccca
     4321 acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacc cgtttattgg
     4381 tctggctgat caccgccgcc aggttggcgc agacaaaggt tttaccgatt gacgggctaa
     4441 ccccggtcat catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc aaactggtac
     4501 gcagactgcg gatggcttca atcgccagat cggttggatt ccccaccgcc agtagctggc
     4561 tctgtttata gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct ttctgccatt
     4621 ccgacagcgg gatgctggca tagacgctga ttccgtgttc ttccagcact tgcgggcttt
     4681 caatgccgcg attaaacaac gagcgcagca gtacacccac gatagagagc atcaggccaa
     4741 ggataatcgc gccgaggata atcagccctt tcttcggttt tagcacacca ggctgggtga
     4801 ttgccgggtc aacaatgcgc acatcgccga cggtgctggc ttcggtgatt ttcagctcct
     4861 gctctttatt cagcagttgc atatagacct gctgaccaga ctcgacatcg cggatcaggc
     4921 gcacaatctc ctgctgggtt ttcggcatcg ccgttacgcg accgttaagt ttggctttct
     4981 cttcttccag cgcctgacgt ttctcctgca gcgtgcggta cgccgggtga actttggtgt
     5041 acagcttgga gatttccgct tctttaaagg tcagttcgtt caactgtgcg tcgatgttca
     5101 ccatcgaatc gagcaccgct ttcgcttcaa gcggcagatc aacagaatct ttatcctgac
     5161 ggaaggcatt cagtttgttt tcggcaacat cgaggcggct acgtacttcc ggtaactgtt
     5221 gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt gcgctcaata ttttgttcct
     5281 gatagttacg ggcgatgctg ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat
     5341 aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccgtcagg ctgttttgca
     5401 gttggttgat catccccaac gtggagtatt tggtgacggt aaactcactg cccgggcggg
     5461 cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg cccgcttgcc
     5521 cacgggcgct aaagccgcca tcactgctca gggtgtagtt tttattgtcc agcacattaa
     5581 gcgtaaacac ctggtccgcc atctctttcg ggcggttaaa ggtggtcact ttcaccgcct
     5641 cgttctgacg tcccatcaaa cgatcccagc ccgcaccgaa aatcgggaac atgtttttgc
     5701 tcaccgcaat atcgaggtcg agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa
     5761 tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc taatgccgaa ccaatgtcct
     5821 gcactaacga attgccgctg ttttgctcga tttgtaccag tgcgtcggca ctataaatcg
     5881 gcgtggcgaa gaaggtgtaa accacggcac agagggcaaa tacagcggtg atgccaatca
     5941 cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt tcatcactgc
     6001 ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct gctctgcgtt
     6061 caatgcctgc gcccactggc gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt
     6121 ttcccggctt ttgcgatacg gatcggggat ttcacattcg ttatcccagt gaccaaacag
     6181 catcactttg ccgcgcatct caggtgccat ctcgcataag cgttcgatat ggcgcttttc
     6241 catggtcaaa atcaggtcat agttgcgaca caggcggcgg ctgatttgtc gtgcacagtg
     6301 gccttccaga gacagttgat gttctgcggc gacgctgata gcggtaggat cagcgccctt
     6361 accgaccagc gcgccgagtc cagcggactc cactttcagt tccgggtgat aacgttgcag
     6421 taagcgttcc gccgtcgggg aacggcaaat attgccgaca cagacaacta atatgttgtt
     6481 aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta acggacaccg
     6541 ctaatagttg gcagcaactg attgatcaga cggttccagc gggaaaccgg ggcggtggtg
     6601 acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag cgacgtcgca
     6661 tcggacatat ccagctggta gatattggca atcttgccgt tacgcccacc ctcgcctttc
     6721 agcggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat accttcggca
     6781 ttgcccagag cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt gctcagtttc
     6841 ttcacttcac ccatcacaaa tactttcaga tcatcattac gcggcacgta gagaatatcg
     6901 ccgggataaa gcagtcagtt ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa
     6961 atgcgttctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc
     7021 aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt
     7081 tgaccggatt tattcacctg acctgagata taggcctttt gtgagcggaa ggcggcgata
     7141 ttaacgtcca cctgcgggtc agcgatgtac gttgctaagc gcccggtaat atcactgcgg
     7201 atttcagcga gcgttttccc cactacgtga accttgccga tatacggata aaacatagtg
     7261 ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg
     7321 gtgagttccg ggtgatccca gacggtgaca ttaagaacgt cccccggccc gacgcgatac
     7381 tggtaattcg cgatctcact ttccagcgtc atattggggc gcgctacatt cgggcgtggg
     7441 cgtaattggt caatcaggcg cggagtcagc ggataaacat tcaccatttt gtcgagatcg
     7501 aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg tcgacatatt gctgcccgga
     7561 agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa tttcattttg
     7621 gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca ctattaatag
     7681 caatgtcgcc atgcacattt accttgcagt taattgaata aaattttaac tggcatcagt
     7741 cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact ggtctttcag
     7801 gcatccagac acgctaccgc ccctggcttt ttagctacca atacactgat ttagcttaat
     7861 ttttaaaacc ctctcaacat acagtcgttg atgagaaagg gttattgcgg aaattaactt
     7921 ccgaatataa ggtgacatta tggtaattga atattggctc tccaataatg caggaggaag
     7981 tgttacagct aacggaatag caggcaaggt aacaattcgg caattggcta tttttaagaa
     8041 ttatattaag tgtcattcaa tatggttttt aggagtttct ttaggttgac aatatttaat
     8101 attgcgtctc cacatgcgat atttcttaaa taatgtttta ttattaccac ttttaattca
     8161 gggataatgt gaggttatta ccctcaaata atatgagata aatagtgctg caacattgca
     8221 tttttgcccc gatttatcca tgatcgaatt gtgacatttg tcatacaacg aataggtttt
     8281 gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat cacgctgatt
     8341 gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct cgccgaaaaa
     8401 ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg
     8461 cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc tgactcaacc gctgttcagc
     8521 ttccgctcgt ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg
     8581 ttgttcaaag ccacgatgga gctgaacgaa cggctggaag ggaaagacag caataatccc
     8641 acacaacgca aaggtgcgaa gttctggggc gtggtgacgc aaattgtggt actggacgcc
     8701 attttctcac tcgactcagt gattaccgcc gtcgggatgg tcgaccattt actggtcatg
     8761 atggccgccg tggttatcgc tatcagcctg atgttgatgg ccagcaagcc gttaacccag
     8821 ttcgttaata gccacccgac catcgttatt ctctgcttaa gcttcctgtt gatgattggc
     8881 tttagcctgg tggcagaagg tttcggcttc gtcattccga aaggctacct gtacgctgcc
     8941 attggtttct cggtgatgat cgaggcactc aatcagttgg ctatctttaa ccggcgacgt
     9001 tttctttccg ctaatcagac gctacgccag cggactaccg aagcggtaat gcgcctgctt
     9061 agcgggcaaa aagaagatgc ggaactggac gccgaaaccg cgtccatgct ggtggatcat
     9121 ggaaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt acttaatctt
     9181 aaccagcgta ccgtcagcag cattatgacg tcgcgccacg atattgagca tatcgatctc
     9241 aacgcgccgg aagacgagat ccgccagttg ctggagcgaa atcagcatac gcggctggtg
     9301 gttaccgacg gtgatgacgc agaagatttg ctcggtgttg ttcacgttat cgacctgcta
     9361 caacagtctc tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca gccgctggtg
     9421 ttcccggaaa ccttgccgtt gttacctgcc ctcgagcagt tccgtaatgc ccgcacgcac
     9481 tttgcttttg tggtggatga gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc
     9541 actgaaacca ttgccggtaa cttaccgaac gaagtggaag agatcgacgc ccgccatgat
     9601 attcagaaga atgcggacgg ttcctggacg gcgaatggtc atatgccgct ggaggatctg
     9661 gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat tgccgggctg
     9721 ttgatggaat atttacagcg tattccaaaa cctggcgaag aagttcaggt gggggattat
     9781 ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aagtgcagat tattccactg
     9841 cgtaaagatg gcgagatgga gtacgaggtg tgatggtatc ggccatttta cccaatagcc
     9901 gaaaggaata agatgaggga accgcgccag cggcgattta ccgccgggag cccgggttgc
     9961 cagggtggtg gcgattgagc caccctggca cgttcaccgg cttgagcgtt ccatagtagc
    10021 gaggaacatc aagtgaacgg aacagccttc gatcttacat attcagaata taatgatgcc
    10081 gccacgaacg cctgatacga cgctttacgc gtcttatcag gcctacagga ctggtcacct
    10141 ggtaacctac atcttctcca gcaacttctt cacatccttg ccattgcggg aatctttatt
    10201 ccgctcggcc cagtcattca ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa
    10261 ctgatccact tgcaggctgt aattgagttg ctgccagttg ccataaaccc gcagcggtac
    10321 tggcgtttct ttcaggaaat caatcagttt actttccccg ttccagccat ccacgacccg
    10381 aatatcaaac tgggtatcgc aggtttgatc tgccagattc aacatgcctt cccctgtcag
    10441 cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt
    10501 caaatcggtg gtaaagcggt caagccgcgt cacgttatca aagttttcag cggccttcac
    10561 atcaccgcca ttacgctcta ccgcttgctg aatcatctgc tggaagttca tcccttccat
    10621 gcgcgtgtcg gtcatttcga catgtgcctg tccttgccag ttgtggcgga atgcgtcggc
    10681 atctatgtca gcaccggaga aatcaccagc cagtgacatt tttccggtca acgaaatcgg
    10741 atagttaaac gccttcagaa tggtaccaat ctcaacgttt tccagccgtg gctggaagtt
    10801 tatccgcgga tttattgatg tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt
    10861 aagcttgccc tgcagttgag taatttccag caaacccgac ttgttggtca tttgcgtggc
    10921 aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca aaatatcagc
    10981 cgtaaagcct tgcagtccct gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg
    11041 cggcaatgtt gattggctct gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc
    11101 attaagcggg atgaggttgt caagattcag ttgcgggaat tgcagcctca gctgccattc
    11161 cggtttctct gtcagagtga cctgtgcttg cccgctcagc gtactgtcat tggcggtcaa
    11221 gctgatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct ggaaactccc
    11281 ctgcccctga ataccttgtt ttggtaaatc ggcaccctgc aactgccagt taatttgttc
    11341 aatagccgcc gttaaatcat gcggataatc agaagcatcc accgtaccgt taagggatat
    11401 tgtgagatcg cgctgatcgc gattaacccg cccggagaac tcaaatgagc cacgatgttg
    11461 gggatcttgt tccatttgca ggcggatatt gcggattgtc acctgctcgt catcttcatg
    11521 ctggaacacc agcacgctgt ccgccacctt aagactggat atatcaaacg accatccgcg
    11581 atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt cttcactgcg
    11641 caccgcttcc gtctgcggcg tcagttggat cactgcccct tttagcatca cctgcttaac
    11701 gctcagttga tgactcagta gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg
    11761 aaccagtggc tggcttgcgc cctgggcggt gagagacatt cgcccggaga ggatactaag
    11821 ctgcggccag acgtgccaac gcagtggccc gtcgagctgc aattgataac cgctacgtgc
    11881 agcaacttgc ttgaccatat agtcgcggaa atcattcgga ttcaccagca acactaacgc
    11941 agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat
    12001 ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg cgccctgctg
    12061 gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg
    12121 ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca gcttaccgga
    12181 gttgtagaac tccagcacaa tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac
    12241 gtgcaccatc agccccagac gcgccagtga ggaacgcccg tccagccagc ccaccagatc
    12301 ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agctctcctg ggtgaagata
    12361 gaacgcctcg ccctcgtcga gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct
    12421 cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg
    12481 aaatttattg cccaggcgta catccaccgt cgcgccgtta atacgctcca ctggcggacg
    12541 tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt cacacagacg
    12601 catggcattt tctcctttcg cgcatcactc ccttaacgcc aattacgtca agggcatacc
    12661 aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga cttattttcg
    12721 ctttcaatat atcgatcgcg atgcggtttt tcccgccgcg cggcacgata atgtccgcat
    12781 attgtttaga aggctcaatg aattgcagga acatcgggcg cacggttttt tgatattgcg
    12841 ccatcactga atccattgaa cgcccacgct cgttaacgtc acgcttgatg cggcgcatca
    12901 ggcagatatc cagcggggta tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg
    12961 catccgtcag caacaaaatg ccttcgagaa tgatgacctt cttcggctca accgtcaccg
    13021 tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc
    13081 gtttcaacgc ttgtaaatgc tcaagcagca gactgtgatc catcgcgctg ggatggtcgt
    13141 agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa tagcagtctt
    13201 cgggaattac gccgatgtgt tcatcaccga cttgctcacg caattcacga taaagggtac
    13261 tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg acgcactgat
    13321 gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga agggggcgac
    13381 gaagcgccaa acgcggcaat tatagggatt tcatccgtct gataccagtc gaatagcgtt
    13441 gccgcgcgct cagagttaat tgttgacaaa gaattcccgg gggcaaatta cgttgatcag
    13501 ttttatgtaa ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct ttacgtcact
    13561 ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact agcataaaca cagaaacgaa
    13621 tactggcgac caggtcttgc ggataaagcg gtaatgagca aacaatcaca gcatgtatta
    13681 attgccctgc cccacccgct gcttcacctg gtcagtttag gtttagtctc gtttatcttt
    13741 acccttttct cgcttgagct ttcgcagttt ggcacccaac tcgccccact gtggttcccg
    13801 acatccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc gggaattgcg
    13861 ctaagctgtt cgctgggaaa tatcgccgca tccatcctgc ttttttccac cagctcgctg
    13921 aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt gctgctacgt
    13981 aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg tctggcactc
    14041 ggcagcgcca ttgttccacc tctgttgggg ggtgttctgg ttgtcctgct gacgcccgga
    14101 gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca
    14161 ctggtgccgc tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccacgg
    14221 ttgctttttg agtcgctgct cacgttagcc atcacactga cgttaagctg gctttcgatg
    14281 ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgt
    14341 ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt gtcgctgatg
    14401 atggccgcgg atccctccct gcttgctacg ccgcgtacgt acctgatgag ccatatgccg
    14461 tggctaccat ttttgctgat cctgctgccc gccaacatca tgaccatggt gatgtatgcc
    14521 tttcgtgcgg aacgcaaaca catttccgaa agcgaaatcc actttcggaa cgcgatggaa
    14581 tattccgcta tcggtatggc gttagtgggc accgagggac aatggctgca aaccaacaaa
    14641 gcgctctgcc agtttctcgg gtacagtcag gaagagctgc gcggactcac ctttcagcaa
    14701 ctgacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct gataagcggt
    14761 gaaataaaca cctattcaat ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg
    14821 gcgttgcttg ccgtctcact ggtgcgccac acggatggca cgccgctcta ttttatcgct
    14881 cagattgaag acattaacga gctaaaacgc accgaacagg tcaatcagca actgatggag
    14941 cgcatcactc tggctaacga agcgggcggg attggcatct gggagtggga gttgaagccg
    15001 aatattttta gctgggataa gcggatgttc gagctgtatg aaattcctcc acatatcaaa
    15061 ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa
    15121 gtgattcgtt gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg
    15181 gtattcgcca tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac
    15241 gtctcctcgg catcaatatg gatatgaccg aagtgaaaca gcttaacgag gcattgtttc
    15301 aggaaaaaga gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg
    15361 atatggcgat gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc
    15421 aggaagaagc gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg
    15481 gaccattaat ggagaacatt tacagtgccg acacctcacg ttccgcgatc gaacaagatg
    15541 tggtgttgca ctgtcggagc ggcggcagtt acgacgtgca ttacagtatt acgccgttaa
    15601 gtactctgga cggcagcaat attggttcgg ttctggtgat tcaggacgtt accgaatcac
    15661 gcaaaatgct gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca
    15721 accgcgccag ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacgcatc
    15781 agcgacatgc actggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg
    15841 ggcatgcggc gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc
    15901 gctccagtga cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg ctgccagact
    15961 gcaatgttga aagcgctcgt tttatcgcta cacgtattat cagtgctgtg aatgactatc
    16021 actttatatg ggaaggccgg gtacatcggg taggtgccag cgccgggatt accttgatcg
    16081 atgacaacaa tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct
    16141 ccaaaaatgg tggccggggc cgggtgacgg tttacgaacc gcagcaagct gccgcacata
    16201 gcgaacgggc ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga
    16261 tgatgctcgc ccacggtgtc gcttcgccac ggatcccgga agcgcgtaat ttgtggctga
    16321 tttcacttaa gctctggagt tgcgaaggcg agattattga tgaacaaaca tttcgtcgta
    16381 gcttcagcga tccggcgctt agccatgctc ttgaccgccg ggtattccac gaatttttcc
    16441 agcaggccgc aaaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg
    16501 ccggtttgag tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc
    16561 taccaccacg gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa
    16621 gcgtgcaaaa actgcggctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc
    16681 tgcaaatctt caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt
    16741 tatgcgccaa cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc
    16801 acgctcagcg actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg
    16861 atacgctttc tggcatcggc gtcgatctga tttatggtga ggtgattgcc gatgcccaac
    16921 cgctggattt gctggtgaat agtagttatt tcgcgattaa ctgaggcgtg cctccccatc
    16981 gcctgatgcg acgctaacgc gtcttatcat gcctacaaat cgctcattcc ccaggccgga
    17041 taaggcgctc gcaccgcatc cggcgaccaa cgtcatgctt cgtctggttg ccagccttcc
    17101 gtataccaga tatgcaacag cgcataagaa cgccagggct tccagcgctc ggcataacgg
    17161 cggatttgcg ccggtgtcat tcccggaaat cgctgtttaa tcagataatc atccggcagg
    17221 aaaacatctt tcgcctgcca gccacgcaaa gcaaaataat tcgccgtcca gcgcccgata
    17281 cccggaaaag tttgcagcgt tttcatcgcc tgctccacat cgcccggtat tgtcattggt
    17341 aaggtgccct ccagcgccgc atttgccaga tgaatcagcg cctctgcccg tttcaacggc
    17401 atacctaacg cttttaatgc ctgcgggtct gctgctgcca gccgctgagg cgtggggaag
    17461 cagatatact ccggaaaatc atccagccgt tcgccataaa gctgtaccac tttggcagtc
    17521 aattttgccg ccatcgccac gctcaccagt tggcctaaaa tcgcccgcac gccctgctca
    17581 aaagcatcaa cactgccggg taaacgcaat ccgggccgcg ccgcgcctaa cctgcccaac
    17641 gcaccgttaa caatttgtgg gttacattgc agatcaaaca ggcggctcat tttcgccaga
    17701 cactctgcgg caactggttc taaacctgca cttaaattta tgtgcagagt atggcgggct
    17761 atatccggaa tagcagtcac cacgccgcga tattcgccca ccgccagact acgggcataa
    17821 taactgtccg cgaccgtttc cacaccgctc accgcacggg cggcgagaaa tcccaacatc
    17881 cacgaccagt catacggcgg ctgccagttc agggtataca tcgcatctcc ttattcatcc
    17941 gctttcagca taaacgttat tcagacgctg cgctttgctt tcatattccg gttgtcgcga
    18001 cggcaacatt tcgctaaagt cacgcccctt cttcaccggc atggggatta tttcgtgttt
    18061 attggttttg attacggtac agcaaactgt tcagtggcgg tcatgcgtga cggtaaaccg
    18121 cacttgctaa aaatggaaaa cgacagcacg ctgctgcctt caatgctttg cgcgccaacg
    18181 cgtgaagcgg taagcgaatg gctgtaccgc catcatgatg ttccggcaga cgacgatgaa
    18241 acgcaggcgc tgttgcgtcg ggcgattcgt tataaccgcg aagaagatat cgatgttacg
    18301 gcgaaaagcg tgcagttcgg tctttcctca ctggcacagt acattgatga tccagaagaa
    18361 gtgtggtttg tgaaatcacc aaaatcgttc ctcggtgcca gcggcttaaa accgcagcag
    18421 gtagcgctgt ttgaggatct ggtctgcgca atgatgttgc acattcgcca gcaggcgcag
    18481 gcgcagctgc cagaagcgat tactcaggcg gtgattggtc gcccgatcaa cttccagggg
    18541 ctgggcggcg atgaagcaaa cgcccaggcg caagggattc tggaacgcgc ggcgaagcgt
    18601 gccggattca gggacgtggt attccagtac gagccggtcg cggctgggct ggattacgaa
    18661 gccaccttgc aggaagaaaa acgggtgctg gtggtggata tcggcggtgg tacgactgac
    18721 tgttcattgc tgctgatggg accgcagtgg cgttcgcgtc tcgatcgtga agccagcctg
    18781 ctgggtcaca gtggttgtcg tattggcggt aacgatctgg atatcgccct ggcgtttaaa
    18841 aacctgatgc cactgctggg tatgggtggc gaaaccgaaa aaggcatcgc cctgccgatc
    18901 ctgccgtggt ggaatgcggt tgccatcaac gacgtacctg cgcagagtga tttctacagt
    18961 agtgccaact gtcgtctgct taacgatctg gtacgcgatg cccgcgaacc ggaaaaagtg
    19021 gccctgttac agaaagtctg gcgtcagcgt ttaagctatc gcctggtgcg tagcgcagaa
    19081 gagtgcaaaa ttgctctttc aagcgtagcg gaaacccgcg cctcactgcc gtttatcagc
    19141 aatgaactgg ctacgctgat tagccagcga gggctggaaa gcgccctcag ccagccactg
    19201 gcgcggattc tggaacaggt gcaactggcc ctggataacg cccaggaaaa accggacgtt
    19261 atctacctga ccggcggtag cgcccgttct ccgctgatta aaaaagcgct ggcagaacag
    19321 ttgccaggca ttccgattgc aggcggcgat gactttggct ccgtcaccgc cgggctggca
    19381 cgctgggcgg aagtggtgtt tcgttaattg aaaaatggct cagcagcggc ttaaatctgc
    19441 ctgcgcctga gccagttttt ctgctgcctg acgtaacggt tccatcaacg gcatggtccg
    19501 tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgactgtca acgccgattc
    19561 cagccgatgt cgtttggcgg tcattttcgc cattaccgct tgtctgtctg ctggcgttat
    19621 cgcttcattc ggcctgaaca caaaacggcg ttcacagctc gctttccagt cgataaccgc
    19681 ctgggtcaga tggtcgccaa accctttcac ttgcttaacg ccacgacggg taacatctgc
    19741 ggctgtttca ataccaaaag accgtaacgc cgctttacgc gcaggaccaa cgccgggaat
    19801 agaggcaaca tcaataaaaa atccctccag aaacttctgc ttctgccgtt cccttgcggt
    19861 gtcgtgaagt gctgccagag cacgtttttc ttcttccggt aacccgagca tttcgtcctt
    19921 cattttttcg agcatcgtcc gtttggcgat aaaaccttcc agcccgccca actgttggat
    19981 ctggctgact aaatggtcat agtccatttt cgcgcgatta aatgcctcac ggcgttgctg
    20041 aacttctgct ttgtacgctt tgcttgtcag actgccaata atccagatag ccgacagcac
    20101 aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcaaca gcgataacgc
    20161 tgacagtgcg atctcaatca gaatgatgta ttcgcgccgt aacaggccta aaggaagcgg
    20221 cctgccagtc ggttggaaat gatcgggtaa tggcaattgc aatgctggcg gtgctactga
    20281 cgccatcacc atcgcccaga ctttcgccag cacaaaatca ccgctggtgg taatgacctc
    20341 ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgcacacc acaggcagtc
    20401 agtcaaatga gcggagtaaa catgcattgc cgaaacggta catttcttta actgttggcg
    20461 tagtaaatca agtgccgcta cccacgcttt tgccgtcgga cgcccggttg ccacgccact
    20521 ttccgtaaac gcctgctgaa acatggcttc aacatcgccc ggtaacatcg ccagcggaat
    20581 agatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg cataacggaa
    20641 atgggcaata tccgtctcca gcgcattacc cgcatcagag ataagcggca caccggaata
    20701 aggatgccga ccaccaaaca agacgtgaaa aatcaacaag gcaaggccaa aattatcgtg
    20761 attttctgta cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg gcggcgtaaa
    20821 atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct gaaaggagtc
    20881 gctatcaatc aacaccactt tgctgtcgcg acctaccata aaactgttct ggtttacgtc
    20941 acccacgacg tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat tgcgcgcaac
    21001 atagagtaga aaatcccacg cacaatgagg gtaactctgg cgacgatgtc ccgggctata
    21061 gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccgg taacttttcc
    21121 gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca actgcgcgtc
    21181 agctgtcgca gccataaagg caagtttgtc ctgttttgag acgggtggcg gcgtgtgata
    21241 aatcttggcg acgctatcga caaactcctc gatatcataa accgcgcctt caccaccttt
    21301 gcccagttca cggcctggcg taacgcactc accagtagca gtatataaag tgggtttcat
    21361 ggtaaccaca gcgccagtgc caacgtttta tcatcatcgg tacgctcgtt taccgccggg
    21421 ctggacaaaa actgctttaa caatttaggt aataaatcga gttgttcctg cgttgctgcc
    21481 gccagtccaa tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc cagcatattt
    21541 aacgccagtc gctgaatacc atccgtaaat gctgcaactt tatgcgcaag ctcagtgctg
    21601 gtgaaagttt ccagtctgga aacagcatct tcatcgctaa tgaaatgcgt catattggca
    21661 tattcaccca ccatcggcgt gagcggcagt tccagaccac gaccaaaatc caccactaca
    21721 ccgccgtcgc caatttgcat aatcaatgtg ccattagccg atgaaatcaa tcccagaaac
    21781 gtgcaggcaa aatcgcgaac tgctaatgct ttggcttctg cctcggcaaa aagtcgttga
    21841 cgaatggtca gtaccatatt tgtggcgaga acatcattaa gccccaattc accgtcctgt
    21901 actttttgca ccataaacgc catcgcttca ttgacggcga gcttcgcgcc ttctcctccc
    21961 tgcgaaacgc tacctgcgcc atcagcaagg aacatcacca gtaatggctg ctggtcattt
    22021 agccaggcaa cctgcatctg gcaggcatcc tgacaaggta aatcagcgct aatgtgcgac
    22081 gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag ccttttggcg
    22141 cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc gaactggata
    22201 accagctaaa cagttcacgg aattgtaatc cctgcagaga caaaggctga cggacgctga
    22261 tttgggccag cgtcttcata tccgccccct gaacaccgat ggaaaagaaa gcaaaccttt
    22321 tatcttcttc cccccgaaac actttgttgg ctgcagcctg ccactcatcg gttggtgcac
    22381 catcggtaat caggaaaatc cacgggcgat aatacgaaat accatttgca cgatactcac
    22441 gtttccgctc ctctaccata tccagggctt tggtaatggc cgcacccatt ggtgtatcgc
    22501 cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt tgttccacat
    22561 gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc ggatcagcaa
    22621 gcagttcatc acgaaaggta accaatccgg cgttaagttc attaataggt ctgccactca
    22681 tcgagccaga aacatccagc agcagaatgc aggggcaacg tggttccggg ttactggcaa
    22741 aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta gagaattatt
    22801 gaggtccggt atcataccga ctgcaataat gaaataagag caaacgaaga aactttcaga
    22861 attaggcccg gcggcgcaga aaattttgct attcgcctcg caagaacgga gagccgtcga
    22921 ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga ggtt
//
LOCUS       DAJTMK010000056        22921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTMK010000056 DAJTMK010000000
VERSION     DAJTMK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.057"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..1083
                     /locus_tag="OO135_001817"
                     /pseudo
     CDS             1..1083
                     /locus_tag="OO135_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            1082..1318
                     /locus_tag="OO135_001818"
                     /pseudo
     CDS             1082..1318
                     /locus_tag="OO135_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="binds to the twin-arginine signal peptides of
                     certain proteins, including dimethylsulfoxide reductase
                     and trimethylamine N-oxide reductase that are translocated
                     to the cytoplasmic membrane; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
     gene            1477..2769
                     /gene="clcB"
                     /locus_tag="OO135_001819"
     CDS             1477..2769
                     /gene="clcB"
                     /locus_tag="OO135_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5397691.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLATGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDCAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTCVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(2722..3417)
                     /gene="bioD"
                     /locus_tag="OO135_001820"
     CDS             complement(2722..3417)
                     /gene="bioD"
                     /locus_tag="OO135_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5397692.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(3542..4762)
                     /gene="mlc"
                     /locus_tag="OO135_001821"
                     /gene_synonym="dgsA"
     CDS             complement(3542..4762)
                     /gene="mlc"
                     /locus_tag="OO135_001821"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5397693.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGIPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTIAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(4897..5790)
                     /locus_tag="OO135_001822"
     CDS             complement(4897..5790)
                     /locus_tag="OO135_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5397694.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLAH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            5897..7149
                     /locus_tag="OO135_001823"
                     /pseudo
     CDS             5897..7149
                     /locus_tag="OO135_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            7545..7859
                     /locus_tag="OO135_001824"
     CDS             7545..7859
                     /locus_tag="OO135_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5397695.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            7952..8035
                     /locus_tag="OO135_001825"
     CDS             7952..8035
                     /locus_tag="OO135_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397696.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            8135..8956
                     /locus_tag="OO135_001826"
     CDS             8135..8956
                     /locus_tag="OO135_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5397697.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGCVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(8995..9324)
                     /gene="mdtI"
                     /locus_tag="OO135_001827"
     CDS             complement(8995..9324)
                     /gene="mdtI"
                     /locus_tag="OO135_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5397698.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(9311..9676)
                     /gene="mdtJ"
                     /locus_tag="OO135_001828"
     CDS             complement(9311..9676)
                     /gene="mdtJ"
                     /locus_tag="OO135_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5397699.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            10088..11121
                     /gene="tqsA"
                     /locus_tag="OO135_001829"
                     /pseudo
     CDS             10088..11121
                     /gene="tqsA"
                     /locus_tag="OO135_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(11146..12534)
                     /gene="pntB"
                     /locus_tag="OO135_001830"
     CDS             complement(11146..12534)
                     /gene="pntB"
                     /locus_tag="OO135_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5397700.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(12545..14077)
                     /gene="pntA"
                     /locus_tag="OO135_001831"
     CDS             complement(12545..14077)
                     /gene="pntA"
                     /locus_tag="OO135_001831"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5397701.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVTRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            14601..15545
                     /locus_tag="OO135_001832"
     CDS             14601..15545
                     /locus_tag="OO135_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5397702.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            15731..17113
                     /locus_tag="OO135_001833"
     CDS             15731..17113
                     /locus_tag="OO135_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5397703.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            17150..17872
                     /gene="folM"
                     /locus_tag="OO135_001834"
     CDS             17150..17872
                     /gene="folM"
                     /locus_tag="OO135_001834"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5397704.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTTDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17869..18204)
                     /locus_tag="OO135_001835"
     CDS             complement(17869..18204)
                     /locus_tag="OO135_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5397705.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            18333..19052
                     /gene="rstA"
                     /locus_tag="OO135_001836"
     CDS             18333..19052
                     /gene="rstA"
                     /locus_tag="OO135_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5397706.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKARHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            19056..20357
                     /gene="rstB"
                     /locus_tag="OO135_001837"
     CDS             19056..20357
                     /gene="rstB"
                     /locus_tag="OO135_001837"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5397707.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            20433..21362
                     /gene="tus"
                     /locus_tag="OO135_001838"
     CDS             20433..21362
                     /gene="tus"
                     /locus_tag="OO135_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5397708.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(21359..22762)
                     /gene="fumC"
                     /locus_tag="OO135_001839"
     CDS             complement(21359..22762)
                     /gene="fumC"
                     /locus_tag="OO135_001839"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5397709.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5547 a         5935 c         5601 g         5838 t
ORIGIN      
        1 atgacgacaa tccgcgacgg cgtccgcggc aaagataaac tggatgtgcc cattaagttc
       61 atctggaact acgcgggaaa taccctcgtt aatcagcatt ctgacatcaa caaaacgcat
      121 gaaattctgc aggacgaatc gaaatgcgaa atgattgtgg tcatcgaaaa ctttatgacc
      181 tcatcggcaa aatatgccga cattctgctg ccagacctga tgaccgttga gcaggaagat
      241 attattccta acgactacgc cggtaacatg ggatatctca ttttcctcca gcctgtcacc
      301 agcgaaaaat tcgaacgcaa accgatttac tggatcctga gtgaagtcgc gaaacgtctt
      361 ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc aggaacaatg gttacaacat
      421 ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt cttacgacga actgaaaaaa
      481 atgggtatct ataagcgtaa agatcccaat ggccattttg tcgcctacaa agcatttcgt
      541 gacgaccgcg aggcaaatcc acttaaaacg ccttccggta agattgaaat ttattccagc
      601 aggctggcgg aaattgcccg tacctgggaa ctggaaaaag atgaagtgat aagcccattg
      661 ccagtttatg cctcaacctt tgaaggctgg aactcccctg aacgtagaac cttcccactg
      721 caactgtttg gtttccatta caaatcccgt actcactcga cctacggcaa tattgatctc
      781 ctgaaggctg cctgccgtca ggaggtgtgg atcaacccta tagatgcgca gaaacgtggg
      841 attgccaacg gcgatatggt gcgggtgttt aaccatcgtg gcgaagttcg gctaccagcc
      901 aaagtaacac cacgtattct ccctggagtt agcgctatgg gccagggagc ctggcacgag
      961 gccaatatgt ctggcgataa aatagaccat ggcggctgtg tgaatacgct aaccactctg
     1021 cgcccttcac cactggcgaa gggaaaaccg cagcacacta atctggtcga gatcgaaaaa
     1081 atattttggt tcactgctgt tgatggctgc gtggttggcg gagaatggtc gccagacaga
     1141 atgcgaagaa ttgctggcat ggcacctttt tccgtggtca acacgttttc ttgatgtttt
     1201 tatcgaaaaa gcagaacacc ctttctaccg tgcactgggt gaactgtctc gcttaacgct
     1261 ggcgcaatgg caatcacaac tgttaattcc tgtcgcggtt aaaccgctgt ttcgatagga
     1321 tcaccgtaat attgccggat ggtgacgatt acgcgcaatc cggcaataat aggttacagt
     1381 gtcacgtttt tttatctctt aaagcacgca ctgcttttgc ggctggcctc ttttgccgca
     1441 aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca catctatccg
     1501 gatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc
     1561 tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt cctcaataat
     1621 gactccggca gtctggtcaa tgcagcgaca aacctttccc cctggcgacg gttgctaact
     1681 ccggcgctcg gcggactggc gacgggtttg ttgctgatgg gctggcagaa atttacccaa
     1741 caacgccctc atgcgccgac cgattacatg gaagcgttgc aaaccgatgg acagttcgat
     1801 tgcgcagcaa gcctggttaa atcgcttgcc tcgctgctgg tagtaaccag cggcagtgca
     1861 attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg ttttgcccaa
     1921 cgttttacgc cacgccagga gtggaaatta tggatcgcct gtggggccgc ggcgggaatg
     1981 gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt gctgtttggc
     2041 actatgatgt tggcctctct cggcccggtg attatttccg ccgtcgtggc attgctggtt
     2101 agcaatctga ttaatcatag cgacgcgtta ctctacaacg tacaactctc agtgacggtt
     2161 caggctcgtg actatgcgct gattatcagt acatgtgtgc tggcaggtct gtgcggacca
     2221 ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg
     2281 ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt cacacctgca
     2341 gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccacc actgttaatg
     2401 atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc
     2461 gcacccggtg gtgtctttac accgacgcta tttatcggtc ttgccattgg catgttgtat
     2521 ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacgctttt actcggattg
     2581 accgagatgg cgacactgtt ggcggcaacc acgcacgcgc cgattatgtc gacgttgatg
     2641 atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc ctgcgtcatt
     2701 gcgtcggtaa tttcgcggac gttacaccgt gactctatct accgccagca cactgcgcag
     2761 catagctaag cggatgtatt gccccagttc tcgctgttcg gcgcgcggca gataaggcag
     2821 ttcaccaatg agcggtgccg gaagtttttt acccagcaca tcaatgattt ccgcataatg
     2881 cgccagtcct gggttgattc ggttagccac ccagccaatg agcggcagcc cgtcgttggc
     2941 aatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac cgacaaccat
     3001 caacaccggc aactgttcct gcaccaccca ttcagagagt ggacgcaaat cattcatcag
     3061 actgcgccag ccgccagtcc cttctaccac gacatgatcg actttatcgg tcaggtttgc
     3121 caggccgttt gaaatgaggg tgtaattgat tgggcaactg tgcgccacgc tactttcttc
     3181 ttcgcttaac gcgataggat taactgcttc ataaggcagt tcgatggttg aaacactctg
     3241 caacaccagg gcatctttat tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac
     3301 gggtttatat cccgcaactg tttttccctg ggaggctaac gcttgtagca atgcgcggga
     3361 aaccaccgtt ttccctacag aagtgtctgt accggtaata aagaaacgct tcagcatcac
     3421 taactccacc gttatgcttc acaaatataa accaggaaaa taattaacct tgaaagtcta
     3481 agttatgctt tcctggccca aattgagata gcgcaaattt tggtataaca gttaaaaaat
     3541 gttaaccctg caacagacga atcaacaaag aaccgttata catcgcgtct tttaccagcg
     3601 cagcgcctgc tatcgtgccc tggttagaaa actgagtact ctcaacgcta atgtgctgac
     3661 tatacgtagg aagggcctgc tgacgtatgc tgtccgagat gacagggaag aggatatctg
     3721 ccgctttact taacggtgag ccaatcagta ttttttgtgg gttaaataaa ttcaccatga
     3781 tggcaagaat gcgcccgaca tgcgcgccca ccccggtaat gatgtctttt gccagtagat
     3841 cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa cggttgccca tgtaacatcg
     3901 agctcatgga ttgattgaga cgcagctgtg ccagctcaag aatactgtcc acactggcga
     3961 tggtttcgag gcagccgtgg ttcccgcaat aacagcgttt accatatggg tcgacctgtg
     4021 tgtggcctat ttccacgaga ctactgctgc ctgcgtgtag cagatgacca tcggtaatga
     4081 cgcccgcccc cacgttgtga tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg
     4141 caccaaacaa ggcctctgcc atcgtccatg cgctgatatc atgctgaata taaaccggta
     4201 tgccggtatg ctgctccagc gcctcgccga gcggcatctc ttttacatcc tcgtagaacg
     4261 gcatgcgatg tacaatacca ttttccgtat caataattcc cggcagggtt atggcaatcg
     4321 aagttagacg ctcaagtttt ttctggtggc ggataaaaaa ctgatcgata tgggaaatga
     4381 tacgatccag caatggcaag tcatctttta acgccagttc ctgcgactct tccaccacca
     4441 gtttgctgct cagatcgcgc agagcaagga aaatctcccc gcgactaatg cgcagagaaa
     4501 gatagtgcca ggcttcagtt tcaaccacca gccccaccgc cggacggcca cggttccccg
     4561 cttctttgat ttccagctct tgcaccaagt gtgcttcgag catctcacgg acaattttag
     4621 tgatactggc aggagccagc tgcgccagac gggaaagatc gatacgcgag actggaccaa
     4681 gctgatcaat caggcgataa accgcgcccg cgttggtctg ctttatttga tcaatgtgcc
     4741 caggctggtt ttcagcaacc accgcatact ccctatattt tcgcgctccg aaataatctg
     4801 taggctatgg tgaagcactt caatacgtgt cgtcaaattt ttacttagac atgtgattaa
     4861 cagcacattt ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat tcagcagatg
     4921 aatacgaaag ttacgcgaag ctggactttg ttcatgatgt ttcggccaga ccaaccacat
     4981 ttcagaaacc gcatcctctt cagcaatcgg cacccagcgc atttcgttga gctgaacacg
     5041 tttaaatgaa gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac cgatgatggt
     5101 cattgcctcg cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc
     5161 gagaatatcg tcatacagcc ctgtcccgac gtgcggatca aaaaagacga agggttcttt
     5221 cgccagttca gccaacgtta cattcgggtt atttgccagg ggatgatcgt gcgggatcat
     5281 cgccataagc ggttcatgga cgatgactgc gtgtgcaagc gtctccggta acgctgtgtt
     5341 acgcagcaat cccatatcca gcgttccttc aatgagcgga gcgatttgct cacgagtgtt
     5401 catttcgcgg gtttgtaaat ggacaccggg ataatcacgg cgaaacagcg ataacgtatc
     5461 ggacaccgcc cgaataaaag gagcggacga agtaaaacca atgcgcaact cccccgcttc
     5521 accctgatgc agtctttcag cgcgagcggc agcgtcatct accatagaca ggatttgccg
     5581 actatctgca agaaactgtt ttcctgctgc cgtcagcaat acactgcgat tagttcgtgc
     5641 cagcagtcgg gcaccaattt gttgctccag tgcctgaatc tgctgactta gcggtggttg
     5701 cgaaatattc aggcgggcag cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa
     5761 gtaacgcaga tgacgaagtt caatattcat atttaaaaca tcttatttga gattattaat
     5821 atattagaca gaacaattcg attttcctaa cctatgtata agcctgatcc acaggcatat
     5881 ttagcaagga tttcaagtga gccgtactac aactgttgat ggcgctccgg caagcgacac
     5941 tgacaagcaa agcatttctc agccaaatca atttattaaa cgcggtacgc cgcaatttat
     6001 gcgcgtcacc ctggcgctgt tctctgccgg actggcaacc tttgcactcc tctattgtgt
     6061 gcagcctatc cttccggtgc tttcgcagga gtttggctta acccccgcga acagtagtat
     6121 ttcactgtcc atttccacgg cgatgttggc tattggtttg ctgtttactg gccccctatc
     6181 cgatgccatt ggtcgcaaac cagtgatggt cacggcgcta ctgttggcct ccatttgtac
     6241 gttatttcga caatgatgac cagctggcac ggcattttga ttatgcgcgc cttgattggg
     6301 ctttcgttaa gtggcgtggc agctgttggc atgacttatc ttagcgagga aatccacccc
     6361 agtttcgtgg ccttttcgat ggggttgtat atcagcggca actcaattgg cggcatgagc
     6421 ggacgcttaa ttagtggtgt cttcacggac tttttcaact ggcgaattgc tctggcggca
     6481 atcggttgtt tcgcgctagc ctcggcgttg atgttctgga aaatccttcc ggaatcacgc
     6541 cattttcgcc cgacttcgct gcgacctaag acgttgttta tcaactttcg tctgcactgg
     6601 cgtgaccggg gattaccgtt attgttcgca gaaggctttt tgctgatggg gtcgttcgtc
     6661 acgctgttta attacatcgg ctatcggttg atgctttccc cctggcatgt cagtcaggcc
     6721 gtggttggct tattatcgct ggcttatttg accggtacac ggagctcacc caaagccgga
     6781 accatgacca cccgctacgg gcgcggtccg gtgatgttgt tttcgacggg ggttatgctg
     6841 tttggtttac tgatgacctt attcagctcg ctgtggctga tctttgccgg aatgttactc
     6901 ttctcagcag gattcttcgc agcccactca gttgccagca gctggatcgg cccccgcgca
     6961 aaacgcgcta aaggccaggc ctcctcgctg tatctgttca gttactatct ggggtcgagt
     7021 attgccggga cgctgggtgg tgttttctgg cataactatg gctggaacgg cgtcggcgca
     7081 tttattgctc tgatgctggt cattgctctg ctggtcggga cgcgtttgca tcgtcgtctg
     7141 cacgcctgaa aaataagccc ggactgcggt aactacacgt ccggacttat tgcttgctca
     7201 aaccaacgtt aatagccatc ctaaaataga cgaagcgcca gccaattccc gcagtgcgtc
     7261 tagcgtcatc aggattataa gtacccaaat aaacggattc attttgctgt gtgtcattta
     7321 ttactgatgc gcagttattc taccgctttg taagtagtaa aatagttaac ccgatcaaga
     7381 ctactattat tggtagctaa atttccctta agtcacaata cgttatatca atgctgtaat
     7441 ttattcagcg tttgtacata tcgttacacg ctgaaaccaa ccactcacgg aagtctgcca
     7501 ttcccaggga tatagttatt tcaacggccc cgcagtgggg ttaaatgaaa aaacaaattg
     7561 agggtatgac aatgaaaaaa gtattagctc tggttgttgc cgctgctatg ggtctgtctt
     7621 ctgccgcctt tgctgcagag actgcgacga ccaccaaagc agcgccggcg aaaacgacac
     7681 atcataaaaa acagcataaa gcagcacctg cccagaaagc gcaggcagct aaaaagcatc
     7741 ataaaaatac gaaagctgaa cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac
     7801 acgccgggaa acacagtcat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag
     7861 ttttcaacag taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcgggta
     7921 acgtttagca ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat
     7981 tcttatcatc cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgactgta
     8041 gttatctgat tttactccca ctttcatccc gtcccgtcta tagtatttat gagggtttgc
     8101 ttttaataat cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt
     8161 gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc
     8221 ggcaaacgat gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga
     8281 cacggcccaa tcaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg
     8341 tacggcgacg ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacacc
     8401 tccaaagggt aaagcggata aagcagtggc gctgcgtttt gtgtcaaata aaggtctttg
     8461 gcgctatgag atccacgaca tagaaggctg cgttgatccg acactgggaa agcggttaaa
     8521 agcagatggg gatggttgga ttgtacctcc cgcagccgcg ccgtgggact tcggattgat
     8581 tgtgctacgt aatccccctt ctggcattac accgttgccg ttatttgagg gagataaagc
     8641 cgcgcttact gccgcattaa aatcggctgg ccgtaaagta actcaggcgg gataccctga
     8701 agatcatctc gatacgttgt acagtcatca aaactgtgaa gtgactggct gggcgcaaac
     8761 gtcggtgatg tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctctgatgtt
     8821 gcataccaat gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga
     8881 tcgctggcgc gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca
     8941 actgtcgcaa aaataatgtt caaacgctgc ccgactgtgc gggcagcgtg ttcatcaggc
     9001 aagtttcacc atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt
     9061 taaccgttga ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc
     9121 ccacaatgca taagcgacag acaagtcgat ccctttaacg gcctgagaaa gcgcactaaa
     9181 ggcagccagc accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga
     9241 aaatttcaaa aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc
     9301 gtgaacccat tcaaactgcg ccatggttca cctccagttc aggtttacgc gctttacggg
     9361 tacctgattt tatcaacaca atcccggcga ccagggtggt taacccggca attttcatca
     9421 gcgataaact ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa ataccgatac
     9481 cttcccacag cgcataagct acgcctaagg cgatcttttt aacggcgaaa gagagaaata
     9541 tatacgatag agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc
     9601 tcgcccattt cattgacagc gtaccggtaa tttctgtagc aatagccagg cctaataaaa
     9661 tccaataaat atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc
     9721 agcgaaatca aagtaaagtg gtcaagcccc gcgcataggg caagcttagg cagaagaaag
     9781 gactaaagcg cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacga
     9841 agatgggtaa atgttgaaaa taaggtcctg aacaaattgt ccataatatt acaattatcc
     9901 gcagtgttgc ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat
     9961 ttgtatcata gcttaagaat taactcaaaa tattttcact tctttacctg agcggtttga
    10021 ttttcgttat gatgacggag cgaaaaagac attattatta gcaaaggaag aaaaaacggg
    10081 gacaagcatg gcaaagccga tcatcacgct caatggccta aaaatcgtca ttatgttggg
    10141 aatgctggtc attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat
    10201 tctcgcatta tttattgctg ttattcttaa cccgctggtg caacacatgg tccgctggcg
    10261 agtgccgcgt gtactggcgg tgtcgatttt gatgaccatc atcgtgatgg cgatggtgtt
    10321 gctattagct tatctgggtt ccgcgctcaa cgagttgacg cggacgttac cgcaatatcg
    10381 caactctatt atgacgccgc tgcaagctct tgagccgttg ttgcaacgcg tagggattga
    10441 cgtctcagtt gaccagctgg cgcattacat tgatccgaac gcagcgatga cgttgctcac
    10501 caacttattg acgcagttat ctaatgccat gtcatcaata tttttattgc tgctgacggt
    10561 gctgtttatg ctgctcgaag tgccacaatt gcccggaaaa tttcagcaaa tgatggcgcg
    10621 tccggttgaa gggatggtgg cgattcaacg tgcaattgac agtgtttctc attatctggt
    10681 gctgaaaaca gccatcagca tcatcaccgg cctggtcgcc tgggcgatgc tcgccgcact
    10741 cgatgttcgc ttcgcttttg tctggggatt gctggccttt gcgcttaatt acatccctaa
    10801 tattggttca gttctcgcgg caatcccccc tatcgctcag gtactggtgt ttaatggctt
    10861 ctacgaagcg ttgctggtgc tggcgggata tctgctgatt aatctggtct tcggcaatat
    10921 tctggagccg cgcatcatgg gacgtgggct ggggctttcc acattggtgg tatttttgtc
    10981 gttgattttt tgggatggtt gttaggaccg gtgggtatgc tgctttccgt gccgttaaca
    11041 attattgtca aaattgcgct tgaacaaaca gcgggaggtc aaagcatcgc cgttctgtta
    11101 agcgatctca ataaagagtg acggcctccg cagaggccgt cagggttaca gagctttcag
    11161 gattgcatcc acgctggctt tggcgtcacc aaacagcatg tgggtgtttt ccttgaagaa
    11221 cagcgggttt tgcacaccag catagccagt gttcatcgaa cgtttaaaga caatcacgtt
    11281 ctgcgctttc cacacttcca gcacaggcat accagcaata ggactcttcg gatcatcctg
    11341 cgccgccggg ttaaccgtat cgttagcacc aatcaccagt acggtatcgg tatcagcaaa
    11401 gtcatcgttg atctcgtcca tttccagcac gatgtcatac ggtacttttg cttcagccag
    11461 caatacgttc atatgtccag gcaaacgccc cgcaaccggg tggataccga aacgcacgtt
    11521 gataccacga gcgcgcagtt tctcggtaat ttcagcgaca ggatattgcg cctgcgcgac
    11581 tgccatgccg taccccggag taatgatcac tgaatgggag tttttcagca gttccgctgt
    11641 ctcttctgcg gtgatttcgc ggtgctcgcc cacttcctga tcatcgccag tagaagagcc
    11701 gtcggtgccg aaaccacccg caataacgct gataaaggaa cggttcatcg ccttacacat
    11761 aatgtaagaa aggatagccc ccgaagaacc gaccagcgca ccggtcacaa tcagcaggtc
    11821 gttgctgagc ataaagcccg cagccgcagc cgcccagccg gagtacgagt tcagcatcga
    11881 caccaccact ggcatatctg caccaccgat ggaggcgact aaatgccagc cgaataccag
    11941 cgcaatggcg gtcattatta gcaatgccag cacttgcagg ccgacgctgt ccgtgcgaac
    12001 aaatacaatc agcagcagga aggaaacgac cagagccgcc aggttcattt tgtgacggtt
    12061 tggcagcatc aacggtttag acgaaatctt gccacacagt ttgccgaacg ccaccaccga
    12121 acccgtgaac gttaccgccc cgatgaagat accgaggaac acttccgtca ggtgaatatt
    12181 gaccagaatc ggtgccattc ccgcgtcatg atgcagatag ctgttaaagc caaccagcac
    12241 tgccgccaga cccacgaagc tgtgcaggat cgccaccagt tctggcattt cggtcatttc
    12301 aactttcttc gccagacgga taccaattgc cccaccaatg accatcgcca gcaagatcca
    12361 gccaacatta cccgtatccg gtccaaaaat ggttgcgatt aacgcaatcg ccatccctgc
    12421 gataccgaag ttgttaccct ggcgagacgt ttcatgtttc gaaagaccgg ccagactgaa
    12481 gataaacagg atcgcggcaa caatgtatgc agctgtaact aatcctccag acatatgtta
    12541 ccccttaatt tttgcggaac attttcagca tgcgctgagt cacggtgaag ccaccgaaaa
    12601 tattaatgct ggctataagc accgcgataa aactaaggaa gctaacccag ccgccctggc
    12661 caatctgcaa cagtgctccg acaacaataa tccctgaaat cgcgttggtg accgacatca
    12721 acggtgtatg cagcgcgtgc gatacattcc acaccacgta ataaccgaca acgcaggcca
    12781 gcgcgaaaac ggtgaagtgc ccaagaaact ctttcggcgc aacgcttgcc agccaaccga
    12841 aaaggatgat tgccagcgcc atcaacgcgt atttacgcca cggtgagcag gcacattttt
    12901 cctcagtttt cacttccggt gccgcttttt gtgccgcctg cggctgagct gatacctgaa
    12961 tcggcggtgc cggccaggta atttcgcccg cacgggtcac ggtcacgcgg cgaatcacca
    13021 catcatcaaa atcaacagtg atattgccgt ctttctcttt gcacaacagt ttcagcagat
    13081 taacgaggtt agtaccgtaa agctgcgagg attgcgtcgg cagacggccc ggaagatcgg
    13141 tataaccaat cactttgaca ccattttccg tagtgaagat ttcacccggc acggtgtatt
    13201 cacagttgcc accgttttgg gctgccaggt cgacaatcac actgcccgtc ttcatggagt
    13261 caaccatttc acgggtaatt agcttcggcg ctggtttgcc tggaataagc gcggtggtga
    13321 caatgatatc gacctctttt gcctgggcgg caaagagttc catttccgct ttaatgaacg
    13381 cgtccgacat cactttggca tagccatcgc cgctgcccgc ttcctcttta aaatccagct
    13441 cgaggaattc cgcgcccata ctttgaactt gttctttcac ttccggacgg gtgtcgaatg
    13501 cacgcacaat cgcgccgaga ctgtttgctg cgccaatggc ggccagacct gcaacacccg
    13561 caccaatcac catcactttt gccggtggca ctttcccggc cgcagtaatt tgcccggtaa
    13621 agaagcgccc aaattcatgt gccgcttcaa caatggcgcg ataaccggcg atgttcgcca
    13681 tcgagcttag tgcgtccagc gattgtgcgc gtgagatacg cggcacagaa tccatcgcca
    13741 tcacggtcac gttacgttcc gcaagttttt gcattaattc cggattctgc gcaggccaga
    13801 taaaactcac cagcgttgtc ccaggattca gtaacgcaat ttcatcatct aacggcgcat
    13861 tgaccttcag aatgatctct gactgccaga cgctattccc ttctacaatt tcagcgcccg
    13921 cttgcacaaa cgctttatcg tcaaaacttg ccagttgacc cgcgccgctc tctaccgcga
    13981 cggtaaaacc cagtttcagc agctgttcca ctgtttttgg cgttgctgca acacgggttt
    14041 cattggttaa ccgttctctt ggtatgccaa ttcgcatgat attcccttcc atcggtttta
    14101 ttgatgatgg tttgcctgtg tcaggagcca cacaagccgc tcatgtacga gctaaatgtt
    14161 actccgttaa aataaattag taacaaacgc cctataacgt actgaaaatt atgcctgtga
    14221 tctagcgcca aaaatcagta tttcggcgtg aactcgcaaa atattaacga ttcagccgtg
    14281 atagtgggat aaacacctta gaacgccgga taaagactga taattgtctt cgacggtcgg
    14341 gtaaaacgag acatcgcccc ggcacgattc actacttaac attaaattaa cttatacaat
    14401 tcagttgctt cagtagtaat gatgctgata cggctgtttt ttaagcatag acggtcattt
    14461 gagcaggatt aaaattggct taaggaatgt gatatgaaaa atgacgcaga cagttacacc
    14521 gtttaaatgc aataatcagc cacgtttctc gttaataaca ataccagtac ctggtttgcg
    14581 caaggcgaag gattattttt atgaagctta agaacaccct cctggcgtcg gcactgcttt
    14641 ctgctacggc attctccgtt aacgcagcaa cagaactgac accggagcaa gcggcagcgg
    14701 ttaaaccttt tgaccgtgta gtggttaccg gtcgttttaa tgctattggc gaagcggtga
    14761 aagccgtttc tcgtcgcgca gataaagaag gtgccgcctc tttttatgtt gtcgacactt
    14821 ctgattttgg taacagcggt aactggcgtg tggtcgctga cctctataaa gccgatgctg
    14881 aaaaagcaga agaaacaagt aatcgcgtaa ttaacggtgt tgtcgaactg ccgaaagatc
    14941 aggctgttct gattgaaccg tttgacacgg tcaccgtcca gggcttctat cgtagccagc
    15001 cagaagtcaa tgatgccatc accaaagcgg caaaagcgaa aggtgcctac tctttctaca
    15061 tcgttcgtca aatcgatgcc aaccagggcg gcaaccagcg tattactgca ttcatctata
    15121 aaaaagatgc taagaaacgt atcgtccaga gcccggatgt gatcccggca gattccgaag
    15181 caggacgtgc agctctggct gccggtggcg aagccgcgaa gaaagttgag atcccaggtg
    15241 ttgcgactac cgcatcacca agttctgaag tcggtcgttt ctttgaaacc cagtcatcaa
    15301 aaggcgggcg ttacaccgtc acgctcccgg atggcactaa agtcgaagaa ctgaacaaag
    15361 cgaccgcagc gatgatggtc ccgttcgata gcatcaaatt ctctggcaac tacggcaaca
    15421 tgaccgaagt ctcctatcag gttgcgaaac gtgccgcgaa gaaaggtgct aagtattatc
    15481 acatcacccg ccagtggcag gaacgtggta ataacctgac cgtcagcgca gatctgtata
    15541 aataactatt cagttactcc tataaggcgg cttgatgccg cctttcgcat ttttagtcac
    15601 aaatttcccc gcgaccattg catgcagaca catctcttcg taaaatcccg cgcttcagtg
    15661 gcaaactcca tttttatgcg ttttgcccta atatttattc ttcttatcac gttttaatca
    15721 ctggatatcg atggaaaaga aactgggact gagcgcactc accgcgctgg tattaagctc
    15781 aatgctgggc gcgggtgttt tcagtctgcc gcaaaatatg gcggcagttg ccagcccggc
    15841 agcactgctc atcggctggg gtattactgg cgctggcatt ttattgctgg cctttgccat
    15901 gctgatcctc acgcgcattc gtcccgaact tgacggcggt atctttacct atgcccgcga
    15961 agggtttggc gagctaatcg gcttttgttc cgcatgggga tactggctgt gcgcagtcat
    16021 cgccaacgtc tcctatctgg tgatcgtttt ttccgcgtta agctttttta cggacacgcc
    16081 ggaattgcgc ctgttcggcg atggtaatac ctggcaatcg atcgtcggtg catcggcttt
    16141 attgtggatt gttcactttt tgatcctgcg cggggtgcaa accgctgcca gcattaacct
    16201 ggtggcgaca ctggcaaaat tgttgccgct gggtctgttt gttgtgctgg cgatgatgat
    16261 gttcaaactg gataccttca agctcgactt caccggactt gtacttggcg tacccgtttg
    16321 ggaacaggtg aaaaacacca tgctgatcac cctgtgggtt ttcattggtg tggaaggtgc
    16381 cgtggttgtt tctgcgcgtg cgcgtaataa acgtgatgtt ggcaaagcga cactgctggc
    16441 ggttctctcc gctctgggcg tttacttgct ggtaacgctg ctttcgctgg gtgtggtggc
    16501 acgtcctgaa ctggctgaaa ttcgtaaccc gtcaatggcc ggtctgatgg tggaaatgat
    16561 gggaccatgg ggcgaaatca tcatcgctgc cagtttgatc gtttccgttt gcggcgcgta
    16621 cttaagctgg accatcatgg cggcagaagt tccgttcctg gctgccactc ataaagcatt
    16681 cccacgcatt tttgcgcgcc agaacgcaca agctgcgcca tccgcctcac tgtggttgac
    16741 caacatctgc gtgcaaattt gtctggtact catctggctg accggttccg attacaatac
    16801 gttgctgacc atcgcctcag aaatgattct ggtgccctat ttcctggtcg gcgcgttctt
    16861 gctgaaaatc gcaacccgtc cactgcataa agcggtaggt gtcggtgcct gcatttatgg
    16921 cttatggtta ttgtacgcct ctggtccaat gcacttgctg ttatccgttg ttctgtatgc
    16981 gccggggctg ctggttttcc tgtatgcgcg caaaacgcat acacatgata atgtactgaa
    17041 ccgtcaggaa atggtgctga ttggcatgct gttaattgcc tcagttccgg cgacctggat
    17101 gctggtggga taacgttcat cccatcgttg gatagcaaag gagataacga tgggtaaagc
    17161 ccagcccttg ccaatattaa ttactggcgg aggtcgtcgc atcggcctcg ccctcgcctg
    17221 gcatttcatt aatcaaaagc aaccggtgat tgtcagctat cggacgcact atccagccat
    17281 tgatggactg attaaggcag gtgcgcagtg tattcaggct gatttttcga ccaccgacgg
    17341 tgtgatggcg tttgccgatg aagtactaaa aagcacccat ggtctgcgtg ctattttgca
    17401 taacgccagt gcgtggatgg cggaaaaacc gggtgcgcca ctggccgacg tactggcttg
    17461 catgatgcag atccacgtta ataccccata cctgctcaac catgcgctgg aaagattact
    17521 gcgtgggcac ggacacgccg ccagcgatat cattcacttt accgattatg tggtggagcg
    17581 cggtagcgac aagcatattg cgtatgctgc aagcaaagcg gcactggata atatgacccg
    17641 ctcgtttgcc cgcaagctgg caccggaagt gaaagtgaat tctattgctc catcgctgat
    17701 cctgtttaat gaacatgatg atgccgaata tcgacaacag gcgctgaata aatcactgat
    17761 gaaaaccgcg cctggcgaga aagaagtgat cgacctggtc gattacttac ttaccagttg
    17821 ctttgtcacc ggacgcagtt tcccacttga tggcggtcgt catctgcgtt aatgcagctt
    17881 tatccagcat ataatcaaca cccatgcgct tatcccccaa cacgcaacag aaccaacaaa
    17941 agcggcgggc aagcgcatca ttccggtgaa ataccacagc gacaccaggt aaacaaaata
    18001 gggaataatc gaccacatac taaaaatgat ggttgcgcgt aaggcttcaa tgccgcgttc
    18061 gctggcaaca atataatgcg cgataagcgc aaaggtcggg aaaagtggaa tcagcccggc
    18121 gatataataa tttttcgttt ttgctaaaac accaatcaac agcactacca gcgcaccgag
    18181 cgcggctttg attaccagcc ccattttttt accttaacac ttccataaca agtcatcagt
    18241 agaatacctg atgaaaactt gtttagaaac gattgatagt aagtaaaaac agcgcggtgt
    18301 attgtgacgt ttttatatct accgtgaatg ttatgaacac tatcgtattt gtggaagatg
    18361 atgcggaagt cggttcactg attgccgcgt acctggcaaa acatgatatg caggttaccg
    18421 tagagccgcg cggcgaccag gccgaagaaa ccattttgcg agaaaatccg gatttggtgt
    18481 tactcgacat catgctacca ggcaaggacg gcatgaccat ttgtcgtgat ttacgcgcaa
    18541 agtggtctgg accgattgtt cttctaacct ctctcgatag cgatatgaac cacatcttgg
    18601 cactggaaat gggtgcctgc gactatattc tcaaaacgat gccccctgct gttttgctag
    18661 cgcgtttacg tttgcatttg cgtcagaatg agcaagccac actgaccaaa ggtcttcagg
    18721 aaacgtctct gactccctac aaagcccggc atttcggcac gttgaccatc gatcccatca
    18781 accgcgtagt caccctggct aacactgaaa tctcgctctc gacagctgat ttcgaattat
    18841 tgtgggaatt agctacccat gccgggcaaa tcatggaccg cgatgcattg ctgaaaaatt
    18901 tacgcggcgt cagttatgac ggactggatc gtaacgtgga cgtggctatt tcgcggttaa
    18961 gaaaaaaact gctcgataac gccgcagaac cttatcgcat taaaactgta cgtaacaaag
    19021 gctatctttt tgcgcctcat gcatgggaat aagcgatgaa aaaactgttt atccagtttt
    19081 acctgttatt gtttgtctgc ttccttgtga tgtctctgct ggttgggttg gtgtacaaat
    19141 ttaccgccga acgcgcgggc aaacagtcgc tggatgattt gatgaacagt tcgctgtatc
    19201 tgatgcgcag cgaattgcgt gagatccccc cacacgactg gggtaagacg ctgaaagaga
    19261 tggatttaaa tctctctttc gatctgcgtg tcgagccact gagtaaatac catcttgatg
    19321 atatttccat gcaccgactg cgtggcggcg aaattgtcgc cctggacgat cagtacacgt
    19381 ttttgcagcg tatcccgcgc agccactacg tgctggcagt tggtcctgtt ccttatcttt
    19441 attatctcca tcagatgcga ttgctggata tcgccctgat cgcttttatt gctatttccc
    19501 tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca ggatatgtta aaactggaag
    19561 cagcggcgca acgatttggc gatgggcatc tctatgagcg tatccacttt gatgagggtt
    19621 cgagctttga acgacttggt atcgcattta accagatggc ggacaatatc aacgccttga
    19681 ttgccagcaa aaaacagctt attgacggta tcgctcacga actgcgaaca ccgttagtgc
    19741 gcctgcgtta tcgactggag atgagcgata acctgagcgc cgccgagtcc caggcgttga
    19801 atcgtgatat cagtcaactt gaagctttaa ttgaagagct gctgacttat gcccgactcg
    19861 atcgcccaca aaacgagctt catcttagcg aaccagacct gccgttgtgg ctgtcaacgc
    19921 atctggcaga tattcaggca gtaacgcccg ataaaacggt acggataaaa acgctcgtgc
    19981 aaggccatta tgcggcgttg gatatgcgct taatggagcg cgtgctggat aatttgctca
    20041 ataacgccct gcggtactgc cattcaacgg ttgaaaccag cctgctactg tcggggaata
    20101 gagcgacatt aattgttgag gatgatggcc cagggattgc cccagaaaac cgcgaacata
    20161 tctttgaacc ttttgttcgc ctcgatccca gccgggatcg ctcaaccggc ggctgcgggc
    20221 tggggctggc aattgtccac tctatagcac tggcaatggg cggtacggtt aattgtgaca
    20281 ccagcgaact gggtggtgct cgcttctcgt ttagctggcc gttatggcat aacatcccgc
    20341 aatttacctc tgcctgacac tacgcgcacg atggtcaagt caataccatt gtgctataaa
    20401 ttaaggatgt tgtaactaaa gtggttaata ttatggcgcg ttacgatctc gtagaccgac
    20461 tcaacactac ctttcgccag atggaacaag agctggctgc atttgccgct catcttgagc
    20521 aacacaagct attggttgcc cgcgtgttct ctttgccgga ggtaaaaaaa gaggatgagc
    20581 ataatccgct taatcgtatt gaggtgaaac aacatctcgg caacgacgcg cagtcgctgg
    20641 cgttgcgtca tttccgccat ttatttattc aacaacagtc cgaaaatcgc agcagcaaag
    20701 ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga taacctttcg caagcagcgt
    20761 tggtcagtca tattcagcac atcaataaac tcaagaccac gttcgagcat atcgtcacgg
    20821 ttgagtcaga actccccacc gcggcacgtt ttgaatgggt gcatcgtcat ttgccggggc
    20881 tgatcaccct taatgcttac cgctcgctca ccgttctgca cgaccccgcc actttacgtt
    20941 ttggctgggc taataaacat atcattaaaa atttgcatcg tgatgaagtc ctggcacagc
    21001 tggaaaaaag cctgaaatca ccacgcagtg tcgcaccgtg gacgcgcgag gagtggcaaa
    21061 gaaaactgga gcgagagtat caggatatcg ctgccctgcc acagaacgcg aagttaaaaa
    21121 tcaaacgtcc ggtgaaggtg cagccgattg cccgcgtctg gtacaaagga gatcaaaaac
    21181 aagtgcaaca cgcctgccct acaccgctga ttgcactgat taatcgggat aatggtgcgg
    21241 gcgtgccgga cgttggtgag ttgttaaatt acgatgccga caatgtgcag caccgttata
    21301 aacctcaggc gcagccgctt cgtttgatca ttccacggct gcacctgtat gttgcagatt
    21361 aacgcccggc tttcatactg ccgaccatct gttctggccg tacccagctg tcaaactcgg
    21421 cttcgctaag ataccccagc gcaagggccg cagcttttaa ggtcagccct tctttatgcg
    21481 cttttttggc gatctccgcg gctttgtcat aaccaatgtg ggtgttaagc gcagtcacca
    21541 gcatcagcga ttcattgagt aattgattga ttcgctcacg attcggttca atacccactg
    21601 cgcagtgttt gttaaaactt tccatgccat ctgccagcaa gcgcaccgat tgcaggaaat
    21661 tatggatcac catcggacgg aagacgttca gttcaaagtt accggaagcg ccacccatgt
    21721 tgatcgccac gtcgttcccc attacctgac agcagagcat ggttaatgct tcacactggg
    21781 ttgggttcac tttccccggc atgattgagc tgcccggctc attttccggg attgagattt
    21841 caccaattcc gcagcgcggg ccagaggcca gccagcggac atcattggcg attttcatca
    21901 gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac cagggcatca caggtcgcaa
    21961 gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca ggtaatgact gccagttcat
    22021 ctgctacgcg acgcgcatac tccggatggg tatttagtcc agtacccacc gctgtaccgc
    22081 caagagccag ttccgctacg tgaggcaggc tgtattcgat atgtttgaga ttatgctcga
    22141 gcatcgctac ccagccggaa atctcctgcc ctagtgttag cggcgtggcg tcctgcaagt
    22201 gggttcgacc gattttgacg atatcggcaa atgcacgcga tttttcactc agtgtctgtg
    22261 tcagggtttt aagctgcgga atgagttgct tgcgcagcgc cagcagcgcc gcaacgtgca
    22321 tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt cacgtcgtcg ttagggtgaa
    22381 ctttacgttc catcccgcgc acgccgccga gtaattcact ggcccggtta gccagcactt
    22441 cgttcatgtt catgttactt tgcgtgccgg agccggtctg ccagatagcc agcgggaatt
    22501 cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg ccgaatggcg ctcgctttct
    22561 cttcagacaa caatcctaaa tcttcattaa cttttgccgc tgcgcgcttg gttagcgcca
    22621 gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat gcggaaatgc tccagcgagc
    22681 gttgagtttg tgcgccccac agcttatctg ccgggacatc aatcgccccc atcgaatctt
    22741 tttcgctgcg tactgtattc atggcctgct cctcacctgt ttaatttttt ctttctgttt
    22801 tgctttcgtt aagcaactct tagctcactt attatttacc atttgataac aaatgtttgg
    22861 tctttcgtgc catgtaaaaa aaccgccccg aagggcgtaa tgccagtcag taaagcaact
    22921 g
//
LOCUS       DAJTMK010000057        22767 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTMK010000057 DAJTMK010000000
VERSION     DAJTMK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22767
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.058"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..334)
                     /locus_tag="OO135_001840"
     CDS             complement(<1..334)
                     /locus_tag="OO135_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5397710.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OO135_001841"
     CDS             complement(415..1842)
                     /locus_tag="OO135_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5397711.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSVLGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPVEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OO135_001842"
     CDS             complement(1839..2546)
                     /locus_tag="OO135_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5397712.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTCGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OO135_001843"
     CDS             2709..3686
                     /locus_tag="OO135_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5397713.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDSNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OO135_001844"
     CDS             complement(3756..4238)
                     /locus_tag="OO135_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5397714.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OO135_001845"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OO135_001845"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5397715.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPIWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OO135_001846"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OO135_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5397716.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSPAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OO135_001847"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OO135_001847"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5397717.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OO135_001848"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OO135_001848"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5397718.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9960
                     /locus_tag="OO135_001849"
     CDS             9349..9960
                     /locus_tag="OO135_001849"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5397719.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10220..10537
                     /gene="rsfS"
                     /locus_tag="OO135_001850"
                     /gene_synonym="rsfA"
     CDS             10220..10537
                     /gene="rsfS"
                     /locus_tag="OO135_001850"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5397720.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10541..11008
                     /gene="rlmH"
                     /locus_tag="OO135_001851"
     CDS             10541..11008
                     /gene="rlmH"
                     /locus_tag="OO135_001851"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5397721.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11039..12940
                     /gene="mrdA"
                     /locus_tag="OO135_001852"
     CDS             11039..12940
                     /gene="mrdA"
                     /locus_tag="OO135_001852"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5397722.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     FRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12943..14055
                     /gene="mrdB"
                     /locus_tag="OO135_001853"
                     /gene_synonym="rodA"
     CDS             12943..14055
                     /gene="mrdB"
                     /locus_tag="OO135_001853"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5397723.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14066..15154
                     /gene="rlpA"
                     /locus_tag="OO135_001854"
     CDS             14066..15154
                     /gene="rlpA"
                     /locus_tag="OO135_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5397724.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGLGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTLATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15294..16505
                     /gene="dacA"
                     /locus_tag="OO135_001855"
     CDS             15294..16505
                     /gene="dacA"
                     /locus_tag="OO135_001855"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5397725.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16616..16879
                     /locus_tag="OO135_001856"
     CDS             16616..16879
                     /locus_tag="OO135_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5397726.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16980..17621
                     /gene="lipB"
                     /locus_tag="OO135_001857"
     CDS             16980..17621
                     /gene="lipB"
                     /locus_tag="OO135_001857"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5397727.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17880..18833
                     /locus_tag="OO135_001858"
     CDS             17880..18833
                     /locus_tag="OO135_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5397728.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIISSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19042..20007
                     /gene="lipA"
                     /locus_tag="OO135_001859"
     CDS             19042..20007
                     /gene="lipA"
                     /locus_tag="OO135_001859"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5397729.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLSQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20108..20311)
                     /gene="tatE"
                     /locus_tag="OO135_001860"
     CDS             complement(20108..20311)
                     /gene="tatE"
                     /locus_tag="OO135_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5397730.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAVAKKGADVDLQAEKLSHKE"
     gene            complement(20440..21228)
                     /locus_tag="OO135_001861"
     CDS             complement(20440..21228)
                     /locus_tag="OO135_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5397731.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGLLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21321..21704
                     /gene="crcB"
                     /locus_tag="OO135_001862"
     CDS             21321..21704
                     /gene="crcB"
                     /locus_tag="OO135_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5397732.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21759..21968)
                     /gene="cspE"
                     /locus_tag="OO135_001863"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21759..21968)
                     /gene="cspE"
                     /locus_tag="OO135_001863"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5397733.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22143..22679)
                     /gene="pagP"
                     /locus_tag="OO135_001864"
     CDS             complement(22143..22679)
                     /gene="pagP"
                     /locus_tag="OO135_001864"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5397734.1"
                     /translation="MFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQPEHYDLYI
                     PAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDSWNKWEPIA
                     GYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYI
                     PGTYNNGNVYFAWMRFQF"
BASE COUNT         5814 a         5709 c         5631 g         5613 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc actggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcacagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggatc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccacagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ctttatctac aaccataagg
     2701 gaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagactcc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattccca tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggttc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccctgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgatga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccagtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc tgcatacgct gctacatggt
     9481 gtttcctttg atctggtttt atgcagtgaa ctggaacggg cacagcatac cgcgcgactg
     9541 gttctcagtg accgccagct ccccgtgcaa atcatccctg aactcaacga aatgtttttt
     9601 ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc
     9661 gcgtggtgca atgactggca gcatgcaatc cccacgaacg gtgaaggatt tcaggcattt
     9721 tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat ttcagcacta tcagaatatt
     9781 ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg cccgtttaat tggcatgcct
     9841 gccgaggcta tgtggcattt tcgcgttgac caggggtgtt ggagcgccat tgatatcaac
     9901 caaaaattcg cgacgctacg cgtcctcaat agccgtgcca tcggggtcga taatgcatga
     9961 cttttctgtt tttttacggg taagccgcaa cgaccattga cagacccggg caggctgata
    10021 ttctccgcag ccagactttt ttcgccagac acgactttgt agaaattgtt ttacaaaaat
    10081 ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg
    10141 acaattgtcc cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg
    10201 attcacccag ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat
    10261 tgatgacctc aaaggtcagg acatcatcgc cttagacgtt caaggcaaat ccagcatcac
    10321 cgactgcatg atcatctgta cgggtacgtc cagccgtcat gttatgtcca ttgctgacca
    10381 cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag
    10441 cgccgactgg attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag
    10501 ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc
    10561 cgtgggaacg aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt
    10621 tccgaaagat atgcccttcg agctgattga aattccggcc ggaaaacgcg gcaagaatgc
    10681 ggacatcaag cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa
    10741 ccgcattgtc accctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga
    10801 gctggaacgc tggaagctgg atggtcgcga cgtcagtcta ctgattggcg ggcctgaagg
    10861 gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg tcgctgtcgg cgcttaccct
    10921 cccccatccg ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac
    10981 caccaaccat ccttatcacc gtgagtgata agggagcttt gagtagaaaa cgcagcggat
    11041 gaaactacag aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc
    11101 gctggtcgcc tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa
    11161 tctgcaaatt tttcgcttta ccgactacca gacccgctct aatgaaaacc gcattaagct
    11221 ggtgcctatc gcgcccagcc gcggcattat ttacgaccgt aatggtatcc ctctggccct
    11281 caaccgcact atctaccaga tagaaatgat gccagagaaa gtcgataacg tgcagcaaac
    11341 gctggacgct ttgcgcagcg tggttgatct gaccgatgac gatattgctg cattccgaaa
    11401 agagcgcgca cgttcacacc gtttcacctc tattcccgtg aaaactaacc tgaccgaagt
    11461 acaagtagct cgctttgccg tcaatcagta ccgttttccg ggggttgaag ttaaaggcta
    11521 taaacgtcgt tactatcctt acggttcggc gttgacccac gtcatcggct atgtgtcgaa
    11581 aatcaacgat aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc
    11641 aacgcatgat atcggtaagc tgggcattga gcgttactat gaagacgtgc tgcacggtca
    11701 gaccggttat gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga
    11761 agtaccaccg caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata
    11821 tattgaaacg ctgctggcgg gtagccgcgc agctgtggta gtcaccgatc cgcgtacagg
    11881 tggggtgctg gcgctggttt ccacgcctag ttatgaccca aacttgtttg ttgacggtat
    11941 ctccagcaaa gattattccg ccttgttgaa cgacccgaat acaccgctgg tgaaccgcgc
    12001 cacacagggg gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt
    12061 gagcgccggg gtgatcacgc gcaatacgac gctgtttgac ccaggctggt ggcaactgcc
    12121 aggttcggaa aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac
    12181 aagatcgctg gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat
    12241 cgatcgcctc tccgaatgga tgggtaaatt cggttatggt cattacaccg gtatcgacct
    12301 ggcggaagaa cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa
    12361 accgtggtat cagggtgaca ccattccggt tggtatcggt cagggttact ggacagcgac
    12421 cccaatccag atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc
    12481 gcatttgctg atgagcaccg ccgaagacgg caaacaggtg ccatgggtac aaccgcatga
    12541 accgcccgtc ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg
    12601 tgttgctaac cgccctaacg gtacggcgca taaatacttt gccagcgcac cgtacaaaat
    12661 tgcggcgaaa tctggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc
    12721 gcacaaaatt gccgagcgtt tacgtgacca caaactgatg accgcttttg cgccatacaa
    12781 caatccgcaa gtggctgtag ccatgattct ggagaacggc ggcgcgggtc cggcggttgg
    12841 tacgctgatg cgtcagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc
    12901 tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa tcatgacgga taatccgaat
    12961 aaaaaaacat tctgggataa agtccatctc gatcccacaa tgctgctgat cttactggca
    13021 ttgctggttt acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg
    13081 gagcgtaaaa tcggccaaat cgcgatgggt ctggtcatca tggtggtgat ggcgcaaatt
    13141 cctccacgcg tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg
    13201 gtggcggtag atgctttcgg tgccatctct aaaggtgctc aacgctggct ggacctcggt
    13261 attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg taccactgat ggttgcgcgc
    13321 tttatcaacc gcgacgtttg cccaccgtcg ttgaagaaca caggcatcgc gctggtgctg
    13381 atatttatgc ccacgctgct ggtggctgca cagcctgacc tgggaacatc aatcctcgtt
    13441 gcgctttccg gtctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc
    13501 gcagtagtgc tggtggcggc gttcattcct attctgtggt tcttcctgat gcatgattac
    13561 cagcgccagc gcgtaatgat gctcctggac ccggaatcag acccactcgg cgcgggctat
    13621 cacattattc agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg
    13681 catggcactc agtcacaact tgaatttcta cccgaacgcc ataccgactt tatcttcgcg
    13741 gtactggcgg aagagctggg attagtgggc attttgattc tgctcgctct ctacattctg
    13801 ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg
    13861 gctggcggat taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc
    13921 ggtattctgc cggttgtagg ggttccgctc ccactggtca gttatggagg atcagcgcta
    13981 attgtgctga tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg
    14041 tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc
    14101 aggaatgctc gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca
    14161 gcctgcggta tgtaacggcc ccatagttga aattagcggg gcggacccgc gtttcgaacc
    14221 actgaacgcg acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca
    14281 ggatccgtct cgatttagcc aggcgggact ggcggcaatc tatgatgccg aaccaggcag
    14341 taacctgacg gcctctggcg aagctttcga tccgacacag ctgacggcgg cccatccaac
    14401 gcttccgatc cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt
    14461 gcgcattaat gatcgcggcc cttacggcaa cgaccgcgtt atttcgcttt ctcgcgcggc
    14521 agctgaccgt cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc
    14581 ccaggatggt tcgctttctg gtcttggtat ggcttgtacc acagtcgcca aacagactta
    14641 cgccctgcct gcacctcccg atttaagcgg tggcgcggga acaagttcag tgtctggccc
    14701 gcagggtgac attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc
    14761 gccggtaacc agtagcggtt tcctcggcgc accaacgacc ttagcgcctg gtgtactgga
    14821 aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca gctccgtcga caacgcttgc
    14881 aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat
    14941 ggtgcaagtc ggggccgtta gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg
    15001 acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct
    15061 tggccccttc gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc
    15121 ccaattacag tcatttatta ccaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa
    15181 tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt
    15241 tgctatagta gggcactttt tttaattcca tcacggatgt cgtagttcag accatgaata
    15301 ccattttttc cgctcgtatc atgaagcgcc tggcgctcac cacggctctt tgcacagcct
    15361 ttatctctgc tgcacatgcc gatgacctga atatcaaaac tatgatcccg ggtgtaccgc
    15421 agatcgatgc ggagtcctac atcctgattg actataactc cggcaaagtg ctcgccgaac
    15481 agaacgcaga tgtccgccgc gatcctgcca gcctgaccaa aatgatgacc agttacgtta
    15541 tcggccaagc aatgaaagcc ggtaaattta aagaaactga tttagtcact atcggcaacg
    15601 acgcatgggc caccggtaac ccggtgttta aaggttcttc gctgatgttc ctcaaaccgg
    15661 gcatgcaggt tccggtttct cagctgatcc gcggtattaa cctgcaatcg ggtaacgatg
    15721 cttgtgtcgc catggctgat tttgccgctg gtagccagga cgcttttgtt ggcttgatga
    15781 acagctacgt taacgccctg ggcctgaaaa acacccactt ccagacggta catggtctgg
    15841 atgctgatgg tcagtacagc tccgcgcgcg atatggcgct gatcggccag gcgttgatcc
    15901 gtgacgtacc gaatgaatac tcgatctata aagaaaaaga atttacgttt aacggtattc
    15961 gccagctgaa ccgtaacggc ctgttatggg ataacagcct gaatgtcgac ggcatcaaaa
    16021 ccggacacac tgacaaagca ggttacaacc ttgttgcttc tgcgactgaa ggccagatgc
    16081 gcttgatctc tgcggtgatg ggcggacgta cttttaaagg ccgtgaagcc gaaagtaaaa
    16141 aactgctgac ctggggcttc cgtttcttcg aaaccgttaa cccactgaaa gtaggtaaag
    16201 agttcgcctc tgaaccggtt tggtttggtg attctgatcg cgcttcgtta ggggttgata
    16261 aagacatgta cctgaccatt ccgcgtggtc gcatgaaaga tctgaaagcc agctatgtgc
    16321 tgaacagcag tgaattgcat gcgccgctgc aaaagaatca ggtcgtcgga actatcaact
    16381 tccagcttga tggcaaaacg atcgagcaac gcccgctggt tgtgttgcaa gaaatcccgg
    16441 aaggtaactt cttcggcaaa atcattgatt acattaaatt aatgttccat cactggtttg
    16501 gttaaaaatt gaacacttga aagtgtaatt tccgtcccca tatactaagc atcagtaaaa
    16561 aaactcccgc cttctggcgg gagttgctat ttaattacgt tacgccggag ctgacatgaa
    16621 aaccaaactt aacgaactgc ttgaattccc tactcctttt acttacaaag ttatggggca
    16681 ggcgttacct gagctggttg atcaggtggt tgaagtggta cagcgccatg cgccaggtga
    16741 ctacacccca acggtaaaac caagcagcaa aggcaactac cactcggtat ctatcactat
    16801 caacgccact catatcgagc aggttgaaac actgtatgaa gaactgggca aaatcgatat
    16861 tgtccgcatg gttctgtaac tcgcttctcc gttacccggc tcctggtcgg gtaactcccc
    16921 tctcctcgct gtgatatact tttcccccac ttttactcat tctccacgga gatgccgttt
    16981 tgtatcagga taaaattctt gtccgccagc tcggtcttca gccttacgag ccaatctccc
    17041 aggctatgca tgaattcacc gatacccgcg atgatagtac ccttgatgaa atctggctgg
    17101 tcgagcacta tccggtattc acccaaggtc aggcaggaaa agcggagcac attttaatgc
    17161 cgggtgatat tccggtgatc cagagcgatc gcggtgggca ggtgacttat cacgggccgg
    17221 gacaacaggt gatgtatgtg ttgcttaacc tgaaacgccg taaactcggt gtgcgtgaac
    17281 tggtgacctt gcttgagcaa acagtggtga ataccctggc tgaactgggt atagaagcgc
    17341 atcctcgggc tgacgcgcct ggtgtctatg tcggggaaaa gaaaatttgc tcactgggtt
    17401 tacgaattcg acgcggttgt tcattccacg gtctggcatt aaacgtcaat atggatcttt
    17461 caccattttt acgtattaat ccttgtgggt atgccggaat ggaaatggct aaaatatcac
    17521 aatggaaacc cgaagcgacg actaataata ttgctccacg tttactggaa aatattttag
    17581 cgctactaaa caatccggac ttcgaatata ttaccgctta attccataca tcaatggccc
    17641 aatttacact gggtcattac tcataatacc tgctaatgct ttacatctcc ttcgcgtttg
    17701 tatattttct ttatcacttg cagaaatgcg gtgccacgcc ttgctccgta actcacccca
    17761 ttgattaacc tattgaaata gttgattatg gtgagcaaaa gcgttccgtt ccaattagac
    17821 ataagattat tgtaattttt taaatattat ttttccatga ataaaattgg agtaagtgcg
    17881 tggatagtaa taatcaaatt gaaccctgtt taagcagaaa gtcgtcagaa ggtaaaccac
    17941 aaatatttac aaccttacga aatattgctc ttaaccttct gactattttt gaagctgtat
    18001 atgtacataa agggatcgtt aatgcagcga aagtgcttaa tctgcccccc tcggcaatca
    18061 gtcagtctat tcagaaactg cgcgttatat tccctgaccc attgtttatt cgcaaaggcc
    18121 agggtgtcac tcctaccgca tttgcgatgc atctacatga gtatatcagt cagggccttg
    18181 agtccattct tggcgcgctg gatatcgaag gaagctatga taagcaacga acgataacta
    18241 ttgcgaccac tccctcggtc ggagccctgg tccttcctgt catctatcgg gcgattaaaa
    18301 ctcactatcc gcagctttta ctgcgcaacc cgcccatcag cgacgcggaa aaccaactca
    18361 gtcagtttca aaccgatctc atcatcgata acatgttttg caccaatcgt acggtgcaac
    18421 atcatgttct gttcaccgac aatatggtgt taatttgccg cgagggaaat ccactactct
    18481 ctttagaaga tgacagagag actatcgaca acgctgcgca tgtacttctg ttaccggaag
    18541 agcaaaattt cagcggtctg cggcagagag ttcaagaaat gtttccggac cggcaaatca
    18601 atttcaccag ctacaacatt ttgacaatcg ctgcactggt tgccaacagt gacatgttag
    18661 cgattatttc cagccgtttt tataacctgt ttagccgctg ctggccgctg gaaaaactgc
    18721 cttttccgtc cttaaataag gagcaaatag atttctctat ccactacaac aaattcagcc
    18781 tgcgtgatcc catcctgcac ggagtaatag atgttatccg taatgcattt tgaaaaagta
    18841 ttgttacagc cagatcacag cgcagaacga taaaatggca caatccaaca acaattttac
    18901 attttcgcga ccgctttggc tgctttcagg tccgtttcaa tgatatactg ccagtcgtta
    18961 attcaaaaat agttgataat tacaacaatc tattgaattg aaacgctttc cttcgtaatt
    19021 cgcaactgga acacgcacgc tatgagtaaa cccattgtga tggaacgcgg tgttaaatac
    19081 cgcgatgccg ataagatggc ccttatcccg gttaaaaacg tagcaataga gcgcgaagcc
    19141 ctgctgcgca agccggaatg gatgaaaatc aagcttccag cggactctac acgtatccag
    19201 ggcatcaaag ccgcaatgcg caaaaatggc ctgcattctg tctgcgagga agcctcctgc
    19261 cctaacctgg cggaatgctt caaccacggc acagcaacgt ttatgatcct cggcgctatt
    19321 tgtacccgcc gttgtccgtt ctgtgatgtt gcccacggtc gcccggtagc tcctgatgcc
    19381 aatgaaccag tgaaactgtc gcagaccatt gccgatatgg cgctgcgtta tgtggttatc
    19441 acctccgttg accgtgatga cctgcgcgat ggcggtgccc agcactttgc ggattgcatt
    19501 actgccattc gggaaaaaag cccgcaaatc aaaattgaaa ctctggtgcc ggatttccgt
    19561 ggtcgtatgg atcgtgctct ggatattctg actgcaacgc caccagatgt gttcaaccat
    19621 aacctggaaa acgtaccgcg tatttaccgt caggtacggc ctggtgcaga ttacaactgg
    19681 tcgctgaagc tgctggaacg ctttaaagaa gcgcatccgg aaatcccgac caagtctggt
    19741 ctgatggtgg gactgggtga aaccaatgaa gaaattattg aggtaatgcg cgacctgcgc
    19801 cgtcatggtg tgacgatgtt aacgctgggg caatatttgc agccaagccg ccatcacctg
    19861 ccggttcaac gttacgttag cccggatgag ttcgacgaaa tgaaagccga agcgctggcg
    19921 atgggcttta cccatgctgc atgcggtccg tttgtccgct cttcttacca cgccgatttg
    19981 caggcgaaag ggatggaagt taagtaatcc ggtaataatt acttacatct gtctgataaa
    20041 aaacccgctt tttgaagcgg gttttttgta tcgaacaaga tattgaggga gcgtcctgct
    20101 cgccacgtca ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt
    20161 tcgccacagc atcatcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt
    20221 ctccgcccag cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta
    20281 ctagcagttt ggtaatacta atctcaccca tagatacctt cttgacatat aaccggcaac
    20341 agaacgcgcc ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca
    20401 atctgtaaca aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc
    20461 aaagcgacgg ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt
    20521 cacttccgcc ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg
    20581 atctataatc cggctttgcc cgatattttt gttcccgcac tcgcccgccg ccaccatata
    20641 acaggtggta tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcag
    20701 cccgcgaacc caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag
    20761 cgccagctct ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccttccac
    20821 ctccagtaac ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa
    20881 tgcatcatag agatgcagtt tggcataacg ggcgacgatg tttcctgcct gaagtgccac
    20941 cagcatattc catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat
    21001 catgttacgt ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc
    21061 tgatttaacc gatagatctg catcatgatc atcgcgtgcc agcaatgctt ccggcaggac
    21121 aaacagcgat acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt
    21181 cttttcccac acagatgtaa cagcaaactg tcctgcagca accagcattg ccacctcctg
    21241 tcagagtcgt aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa
    21301 tcaaatagca ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta
    21361 cgggaagcgt ggcgagatgg ctgttaagta tgcgatttaa cccactgcat caggcgattc
    21421 cgttggggac gctgacagca aacctgattg gggcattcat cataggaatg ggattcgcat
    21481 ggttcagcag gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt
    21541 gtggcggtct aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg
    21601 gtcgctttgg ctgggcatta ctgaacgttt tcgtcaacct tctggggtct tttgccatga
    21661 cagcactggc tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaacc
    21721 cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca
    21781 gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga
    21841 gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt gctgccgtct
    21901 tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc
    21961 ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt
    22021 acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt
    22081 tatttttgat gttacttatt ttagctattg attttaaaga agttactaaa acttcatttg
    22141 tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga
    22201 atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc
    22261 gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct
    22321 aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc
    22381 ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct
    22441 ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt atagcgatcg
    22501 gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca
    22561 taatgttcag gctgttgcca ggttggtgca atattttctc taaacgttgt catccactca
    22621 tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa ggaaaagata
    22681 gcgacatatt ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
    22741 ctccagtggc ttctgtttct atcagct
//
LOCUS       DAJTMK010000058        22760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTMK010000058 DAJTMK010000000
VERSION     DAJTMK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22760
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.059"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1005
                     /locus_tag="OO135_001865"
     CDS             <1..1005
                     /locus_tag="OO135_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5397735.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRVYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
     gene            complement(1007..1339)
                     /locus_tag="OO135_001866"
     CDS             complement(1007..1339)
                     /locus_tag="OO135_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5397736.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    1579..1651
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            1645..2058
                     /gene="ibpA"
                     /locus_tag="OO135_001867"
     CDS             1645..2058
                     /gene="ibpA"
                     /locus_tag="OO135_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5397737.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            2170..2598
                     /gene="ibpB"
                     /locus_tag="OO135_001868"
     CDS             2170..2598
                     /gene="ibpB"
                     /locus_tag="OO135_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5397738.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            2794..4455
                     /locus_tag="OO135_001869"
     CDS             2794..4455
                     /locus_tag="OO135_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5397739.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALSLGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(4452..5168)
                     /locus_tag="OO135_001870"
     CDS             complement(4452..5168)
                     /locus_tag="OO135_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5397740.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEFQVEYHLRRLHPEKS"
     gene            5464..7080
                     /locus_tag="OO135_001871"
     CDS             5464..7080
                     /locus_tag="OO135_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5397741.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            7080..8403
                     /locus_tag="OO135_001872"
                     /pseudo
     CDS             7080..8403
                     /locus_tag="OO135_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(8534..8965)
                     /gene="tnpA"
                     /locus_tag="OO135_001873"
                     /pseudo
     CDS             complement(8534..8965)
                     /gene="tnpA"
                     /locus_tag="OO135_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8973..10154
                     /locus_tag="OO135_001874"
                     /pseudo
     CDS             8973..10154
                     /locus_tag="OO135_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            10337..10684
                     /locus_tag="OO135_001875"
     CDS             10337..10684
                     /locus_tag="OO135_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5397742.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            10674..11036
                     /locus_tag="OO135_001876"
     CDS             10674..11036
                     /locus_tag="OO135_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5397743.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            11033..11530
                     /locus_tag="OO135_001877"
     CDS             11033..11530
                     /locus_tag="OO135_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5397744.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTILR"
     gene            complement(11538..12722)
                     /gene="emrD"
                     /locus_tag="OO135_001878"
     CDS             complement(11538..12722)
                     /gene="emrD"
                     /locus_tag="OO135_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5397745.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLCHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(13141..13230)
                     /gene="tisB"
                     /locus_tag="OO135_001879"
     CDS             complement(13141..13230)
                     /gene="tisB"
                     /locus_tag="OO135_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5397746.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13795..13893
                     /gene="ivbL"
                     /locus_tag="OO135_001880"
     CDS             13795..13893
                     /gene="ivbL"
                     /locus_tag="OO135_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5397747.1"
                     /translation="MTTSMFNAKLLPTALSVAVVVVRVVVVVGNAP"
     gene            13999..15687
                     /gene="ilvB"
                     /locus_tag="OO135_001881"
     CDS             13999..15687
                     /gene="ilvB"
                     /locus_tag="OO135_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5397748.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            15691..15981
                     /gene="ilvN"
                     /locus_tag="OO135_001882"
     CDS             15691..15981
                     /gene="ilvN"
                     /locus_tag="OO135_001882"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5397749.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            16054..16644
                     /gene="uhpA"
                     /locus_tag="OO135_001883"
     CDS             16054..16644
                     /gene="uhpA"
                     /locus_tag="OO135_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5397750.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            16644..18146
                     /gene="uhpB"
                     /locus_tag="OO135_001884"
     CDS             16644..18146
                     /gene="uhpB"
                     /locus_tag="OO135_001884"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5397751.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGHG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            18156..19475
                     /gene="uhpC"
                     /locus_tag="OO135_001885"
     CDS             18156..19475
                     /gene="uhpC"
                     /locus_tag="OO135_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5397752.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            19613..21004
                     /gene="uhpT"
                     /locus_tag="OO135_001886"
     CDS             19613..21004
                     /gene="uhpT"
                     /locus_tag="OO135_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5397753.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLCFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(21049..>22760)
                     /gene="adeD"
                     /locus_tag="OO135_001887"
     CDS             complement(21049..>22760)
                     /gene="adeD"
                     /locus_tag="OO135_001887"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5397754.1"
                     /translation="LLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVG
                     AEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHE
                     IVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQ
                     VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHES
                     YQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEG
                     HIDALIRRLIEQYNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVT
                     VQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVI
                     DVIHNELITHSHSSVYSENGFDRDDVYFIAVLERYGQRLAPACGLLGGFGLNEGALAA
                     TVSHDSHNIVVIGRSAEEMALAVYQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTA
                     QSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLE
                     VTE"
BASE COUNT         5197 a         5849 c         6211 g         5503 t
ORIGIN      
        1 ccgaagtcag agaaatatgt tttcatccat ggcccggaaa atcctgatga aacatggtat
       61 tacgatttcc atcatcgacg cggagtcatt gctgaaggcg gcaaggtgag caatctcgat
      121 gcaatggata ttaccgcgcc gtatacgcca ggagcgctgc gcggcggcag ccatgtgcat
      181 gtctttagcc cgaacggtga aagggtgagc tttacctata acgaccatgt aatgcatgaa
      241 ctcgatccgg cgctggattt gcgaaacgtc ggtgttgctg cgccgtttgg cccggtcaac
      301 gtacaaaagc agcatccgcg tgaatacagc ggtagccact ggtgcgtgct ggtgagtaaa
      361 accacgccca cgccgcagcc tggcagcgat gaaatcaatc gtgcttatga agaaggatgg
      421 gtaggaaatc acgcgctggc gtttattggc gacacacttt cgccaaaggg cgagaaagtg
      481 ccggagctgt ttatcgttga gttaccgcaa gatgaagctg gctggaaagc ggcaggtgat
      541 gcgccgttaa gtggaacgga aaccaccctg cccgcgccac cgcgcggcgt cgtgcagcga
      601 cgtttaacct ttacccacca tcgggtttat ccggggttag tcaacgtccc gcgccactgg
      661 gtgcgctgta atccgcaggg tacgcaaatc gcgtttttaa tgcgtgatga taacggcatt
      721 gtgcaactgt ggcttatctc gccacagggc ggcgagccgc gccagttaac ccataacaaa
      781 acggatattc agtctgcatt taactggcat ccgtcaggag aatggttggg ctttgtgctg
      841 gataatcgaa ttgcttgtgc ccatgcgcaa agtggcgagg ttgagtattt aaccgaaaac
      901 cacgccaatc caccttctgc ggacgccgtg gtcttctcgc cggatggtca atggctggcg
      961 tggatggaag gcggccagct gtggatcacc gaaactgatc gctaaatcag ttatcaggca
     1021 ttctggccgg tggaataacc gcattggtgg cttgcgcgtt ggcttcgctt ttttcaacgc
     1081 gtgagcgcac cgagctgtct ttgcggaaca catcccacgg cagtaaaagc gtgtccatca
     1141 cagcggtaaa cggcatatcg agaatcgcca gcgacttagt gccccagttg gtttcatcgt
     1201 cgccaatcat ggttgcgctg gcgcgggtgc cgggatacgt tccttctttc cctccggtat
     1261 gggacatcac gctggagcat cctccgagta atgtcattcc gctgcatatc atgaacgcta
     1321 acaacacatt tcttatcata ataaattcat ctgttgatcg tgggtgttgg cctgatgagt
     1381 tatagcgctc ccttgctgaa aataacatca tcattacgtc gcactgtggc ggctatcgca
     1441 ctttaacgtt tcgtgctgcc ccctcagtct atgcaataga ccataaactg caaaaaaaag
     1501 tccgctgata aggcttgaaa agttcatttc cagacccatt tttacatcgt agccgatgag
     1561 gacgcgcctg atgggtgttc tggctacctg acctgtccat tgtggaaggt attacattct
     1621 cgctgatttc aggagctatt gattatgcgt aactttgatt tatccccgct ttaccgttct
     1681 gctattggat ttgaccgttt gtttaaccac ttagaaaaca accagagcca gagtaatggc
     1741 ggctaccctc cgtataacgt tgaactggta gacgaaaacc attaccgcat tgctatcgct
     1801 gtggctggtt ttgctgagag cgaactggaa attaccgccc aggataatct gctggtggtg
     1861 aaaggtgctc acgccgacga acaaaaagag cgcacctatc tgtaccaggg catcgctgaa
     1921 cgcaactttg aacgcaaatt ccagttagct gagaacattc atgttcgtgg ggctaacctg
     1981 gtaaatggtt tgctgtatat cgatctcgaa cgcgtgattc cggaagcgaa aaaaccgcgc
     2041 cgtatcgaaa tcaactaata ccctaaggcc gcctgacgcg gcctgacatc tccatgctcg
     2101 ccgtcaggga gcatatgcga atcttcggat ttgcaggtac ttactcgctt cttagaagga
     2161 gaaatgacta tgcgtaactt cgatttatcc ccactgatgc gtcaatggat cggttttgac
     2221 aaactggcca acgcactgca aaacgccggt gaaagccaga gcttcccgcc gtacaacatt
     2281 gagaaaagcg acgataacca ctaccgcatt acccttgcgc tggcaggttt ccgtcaggaa
     2341 gatttagaga ttcaactgga aggtacgcgc ctgagcgtaa aaggcacgcc ggagcagcca
     2401 aaagaagaga aaaaatggct gcatcaaggg cttatgaatc agccatttag cctgagcttt
     2461 acgctggctg aaaatatgga agtctctggc gcaaccttcg taaacggttt actgcatatt
     2521 gatttaattc gtaatgagcc tgaacccatc gcagcgcagc gtatcgctat cagcgaacgt
     2581 cccgcgttaa atagctaact agctattctc tttgccccgc cattctggtg gggctttttt
     2641 tgtctttctt cttcaacaat gcgttaaatt tcgactgttt aagatatttc ggcacgttct
     2701 ttcccgtgat aatgtgcgct ataaggcaaa tctgcttcac cgattcagcc ataatccgta
     2761 gcaattaaaa tgttatctca agagaaatgg acgatgagtg atatagcatt aacggtcagt
     2821 attctggctt tggtggcagt cgtcggtttg tttatcggca acgtcaaatt tcgcggcata
     2881 ggattaggta ttggcggcgt gctgtttggt gggatcatcg tcggccattt tgtttctcag
     2941 gcggggatga cattaagtag cgatatgctg catgttattc aggaatttgg cctgatcctg
     3001 ttcgtttata ccatcgggat tcaggtaggg ccgggcttct ttgcctcatt gcgcgtctcc
     3061 ggattacgcc tcaacctgtt tgctgttctg atcgtcatca tcggtggtct ggttaccgcc
     3121 atcctgcata aactgtttga tattccactg ccggtagtgc tggggatttt ctccggtgcg
     3181 gttaccaata cgccagcgct gggggcaggg cagcagatct tgcgcgacct gggtacacca
     3241 atggaaatgg tcgatcagat ggggatgagt tacgcgatgg cgtatccatt cggcatttgc
     3301 gggattttgt tcaccatgtg gatgttgcgg gttattttcc gcgtcaatgt cgagacagaa
     3361 gctcagcagc acgagtcttc acgcaccaat ggcggcgcgc tgatcaagac tatcaatatt
     3421 cgcgttgaga accctaacct gcatgattta gccattaaag atgtaccgat tctcaacggc
     3481 gacaaaatta tctgctcgcg tctgaaacgc gaagaaaccc taaaagttcc ttcgccagat
     3541 accattatcc aactgggcga tttgctgcat ctggtggggc agccagcgga tttacataat
     3601 gcgcaactgg tgattggtca ggaggtcgat acttcgctgt ccacgaaagg cactgatttg
     3661 cgcgtcgagc gtgtggtggt caccaatgaa aacgtgctcg gaaaacgtat tcgcgacctg
     3721 cactttaaag aacgctatga cgttgttatc tcgcgcctga accgtgccgg ggtcgaactg
     3781 gtcgccagtg gcgatatcag cctgcagttc ggcgatatcc tcaatctggt ggggcgtccg
     3841 tccgcaattg atgccgttgc caatgtgctg gggaatgcgc agcaaaaact gcaacaggtt
     3901 cagatgctgc cagtgtttat tggcatcggg cttggcgtat tgttaggttc tattcccgtc
     3961 tttgtgcctg ggttcccggc cgcgttgaaa ctggggctgg cgggcggtcc gctgattatg
     4021 gcgttgagcc tcgggcgtat cggcagtatc ggcaagctgt actggtttat gccgccaagt
     4081 gccaacctcg cgctgcggga gctggggatc gtgctgttcc tctcggtcgt tggtctgaaa
     4141 tctggtgggg attttgtgaa taccctggtc aatggcgaag ggctaagctg gattggttat
     4201 ggtgccctga tcaccgccgt tccgctgatt actgttggca ttctggcgcg gatgttagcc
     4261 aaaatgaatt acctgaccat gtgcgggatg ctggcaggtt ccatgaccga tcctccggcg
     4321 ctggcgtttg ctaataatct tcatccaacc agtggtgcgg cggcgctctc ttacgccact
     4381 gtctatccgt tagtgatgtt tctgcgaatt atcacccccc aattactggc ggtgctcttc
     4441 tggagtatcg gttaactctt ttccgggtgg agtcgccgta aatggtactc cacctggaat
     4501 tcgctggcat ttctgaacat cactgaataa ttcaaaaact ccccgctctc gctatatgac
     4561 aacgaggtga tccgcagcag tggcgtgtgt tccgctacct tcatttgccg cgccagcagt
     4621 ctatcggcga gcaccggcgt caggctttca taattaccgc caatcaaaat cccacactct
     4681 tgttcaatat attcaaactt cgacccttcc agatgttgca gcgaaagatt acggaacagt
     4741 ttaaccggca tatagctgtc ttccagcatc agcggtttcc cttccacaaa acgaacgcga
     4801 cgggagaagt agatctgctc gttgatttga atccgtaact ggctggcaat ggccggaggc
     4861 gcaggcatga tttcaaaaat cagcacctgg ctggtgaccg tttttccctg ccgttttaac
     4921 acctccacca gtccggtcag gctggcggtt tgatgcagca catctttgcg caccaggtaa
     4981 gtgccgctgc cgtggcggcg gaccaccagc ccccacgcta ccagcaggtc aatcgcttta
     5041 cggatggtca tccgggatac tgcaaattct tccgccaact ttttttcacc gggaagaagg
     5101 ctgtttagcg tgaaatccgc ggaattaagt cgaattctta accgctccgc aatgcttttg
     5161 tagatcatct ctagatctct tttttgcctg caagcccggg taatgcgccc acaagttctg
     5221 cttaatcgat tgaaaaaaca tctttttttt aaagatgtgt tcgatgctgt gtaccagtgc
     5281 tcacagatgt ctactttttc gcgaaaacgt agatctctac cacccaacga aaagcatgaa
     5341 agcgatcacg aatcccattg ggtcgtcatg ttctgccgat cgcatcttcc tatcctcgct
     5401 ccaggcctgc cgcataacca atcaggcttc ctacttacag aattgagaaa agaggatgtg
     5461 gaaatgctca gtcaaattca acgctttggc ggcgcgatgt tcacgccagt gctgctgttt
     5521 cccttcgccg ggattgtggt gggtcttgcc atcttgctgc aaaacccgat gtttgtcggg
     5581 gaatcactga ccgatccgaa cagtttattc gcgcaaatcg tacacattat tgaagagggc
     5641 ggttggacgg tattccgtaa tatgccgctg atttttgctg tcggtttacc cattggcctt
     5701 gctaagcaag cgcaggggcg tgcttgtctg gcggtgatgg tgagtttcct gacttggaac
     5761 tatttcatca acgcgatggg aatgacctgg ggaagctact tcggcgtcga tttcactcag
     5821 gacgcggtgg caggtagcgg tctgacaatg atggccggga ttaaaaccct cgataccagc
     5881 attatcggcg caattatcat ttccggcatt gtgacggcgc tgcataaccg tctgttcgat
     5941 aaaaaactgc cggtttttct cggcattttc caggggacgt cttatgtggt gattatcgcc
     6001 ttcctggtga tgatcccctg tgcctggctg acgttgctcg gctggccaaa agtacaaatg
     6061 gggattgaat ctctgcaagc gttcctgcgt tcggcgggtg cgcttggggt gtgggtttac
     6121 accttcctcg aacgtattct gatcccaacc ggtttacacc acttcatcta cggacagttt
     6181 atctttggtc cggcagctgt tgaaggcggc attcagatgt actgggcgca gcatctgcaa
     6241 gagttcagtc tgagcgccga gccgctgaaa tcgttgttcc cggaaggcgg ttttgccctg
     6301 cacggtaact caaaaatctt tggtgccgtg ggcatttctt tagcgatgta cttcactgcc
     6361 gcaccggaaa atcgggtaaa agtggcgggc ttgctgattc ccgcaacctt aaccgccatg
     6421 ctggtgggaa ttaccgaacc gctggaattt accttcctgt tcatttcacc gttgctgttt
     6481 gcggtacacg ccgtgctggc ggcctcaatg tcgaccgtga tgtatctctt tggtgtggtg
     6541 ggcaacatgg gcggaggtct gattgaccag gttttaccgc aaaactggat cccgatgttc
     6601 agcaaccacg cggatatgat gctgacccaa atcgccattg ggttgtgctt taccctgctg
     6661 tacttcgtgg ttttccgcac actgattctg caattcaaca tgtgcacgcc gggacgtgaa
     6721 gatgcggaag tgaaactcta ctcaaaagcc gaatacaaag cctcgcgagg ccaaaccacc
     6781 gctgcagagc caaaaaaaga gctggatcag gctgccggta tcctgcaagc cctgggcggg
     6841 gtcggcaata tctccagcat taacaattgc gcgacgcgtt tacgtattgc actgcatgac
     6901 atgtcacaaa cgctggatga cgaagtcttt aaaaagctgg gagcgcacgg cgtcttccgt
     6961 agtggcgatg ccattcaggt gatcattggt ctgcatgtat cccagctgcg tgaacagctc
     7021 gatagcttaa ttaattctca tcaatcagca gaaaatgttg ccattacgga ggcagtataa
     7081 tgaccaaatt ctcagtggtt gtcgcaggcg gtggaagcac ctttacgcca ggcatcgtgt
     7141 tgatgctcct ggcgaatcag gaccgtttcc cgcttcgtgc actgaaattt tatgataacg
     7201 atggtgcgcg gcaggaagtg attgccgaag cctgtaaagt catccttaaa gagaaagcgc
     7261 cggacattgc gtttagttac accaccgatc ctgaagtggc attcagcgac gttgattttg
     7321 tcatggcgca catccgcgtc ggcaaatacc cgatgcgcga actggatgaa aaaatcccgc
     7381 tgcgccacgg cgttgttggt caggaaactt gcggacccgg cggaatagcg tacggcatgc
     7441 gttccattgg cggcgtcctg gaactggtgg attatatgga aaaatattca ccaaatgcct
     7501 ggatgctcaa ctactccaac ccggcagcaa ttgtcgcaga agccacgcgt cgtctgcgcc
     7561 cgaatgcgaa aatcctcaac atctgtgaca tgccaatcgg tattgaaagc cggatggcgc
     7621 aaattattgg gctgcaagat cgcaaacaga tgcgcgtgcg ctactacggc ctgaaccact
     7681 ttggctggtg gacatcaatt gaagatttgc agggcaacga cctgatgccc cagctgcggc
     7741 aatatgtctc taagcacggt tatgttccac cgcagcaaga tacacatact gaagcgagct
     7801 ggaacgacac ctatgcaaaa gcgcgggatg tccaggcact ggacccggat acattgccga
     7861 acacctatct gaaatattat ctcttcccgg attatgttgt tcagcattcc aaccctgaac
     7921 atacccgcgc gaatgaggtg aatggaacat cgcgagaaac aggttttcga tgcttgccgc
     7981 gccattacag cggcaggaaa ttcagcggcg gccaagctgg agattgacga acatgcgtca
     8041 tacatcgtcg atctggcggc ggcaattgcc ttcaacactc aggagcggat gttgctgatt
     8101 gtgcctaaca acggggcaat ccataacttt gatgatgaag cgatggtcga gatcccgtgt
     8161 ctggtcgggc acaacggacc agaaccactg gtggtcggcg atatcccgca gtttcagaaa
     8221 gggttaatga gtcagcaagt ggcggtggaa aaactggtcg tggacgcctg ggaacagcgt
     8281 tcatatcagc acctgtggca ggcgattacg ttatcgaaaa cggtaccgag cgcctcggtc
     8341 gccaaagcta ttctggatga gttgctggag gccaacaaag cgtactggcc agagttacgt
     8401 taattgtgtg gactggcatc cgttgggtgc tggtccgtat tctttagcac ggctgacggc
     8461 gcataaccaa atttatccgg tgcgccgtaa aaccccgtcc ttcagggcgg ggatataagg
     8521 cgcggttttc cacctaacca ggtgtttgct gttgttcaat gtattggcgg atgacggata
     8581 ttggcgcacc tccgcagcta ttggcaaaat agccaggact ccacaaaacg cctttgtagt
     8641 aatacctgac tgcaatatct ggtagatctc gtcgcagtca cctacccgat acgcctttga
     8701 ggctgtttac cagactggat atcgccagtt tgggaggata gttaattaac aaatggacgt
     8761 gatctggttc gccatccatt taaaccagtt cagcttcaaa atcagcacat acatttgaaa
     8821 agtaagtgcg tagtttttct gtcgcgtcat agtcaaaaat ctgacgtcgg tatttggtga
     8881 caaagaccag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg atatcggttt
     8941 cttttttcac agaccaaagt atcatttggc ctgtgaaacg actacaagca tttaaattcc
     9001 agttaagacc cggtggtcaa caggagcgtg aaatgaggcg cttcgcaggc gcatgtcgtt
     9061 tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca tgaggccggt aataaataca
     9121 tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact gaaacgcaat
     9181 ggcttaaaga tgccccgtca cagccattgc aacagtcact gaaagacctt gagcgggctt
     9241 acaaaaactt cttccagaat cgggctgctt ttccccgatt caaaaagcgg ggacagaatg
     9301 atgtattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt atttttctgc
     9361 cgaaactggg ctggatgcgc taccggaaca gccgttaggt cacgggtgtt gtgaaaaatg
     9421 tcactgtcag ccagtcctgc ggtaagtggt acatcagtat tcagacagaa agtgaagtat
     9481 caactccggt tcacccttca gcatcaatgg tcgggctgga tgctggcgtg gctaaactcg
     9541 ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa aaccagaaga
     9601 agctggcgag acttcagcga cagttaagcc gcaaggtcaa atttagcaac aactggcaga
     9661 agcagaaacg caaaatacgc gactgcattc ctgtatcgca aatatccgca gggactacct
     9721 tcacaaagtc acaacgaccg tcagcaaaaa acacgcaatg atagtcattg aggatttgaa
     9781 ggtcagcaac atgtcaaagt cagcagcggg tacggtcagc ctgccggggc gcaatgtccg
     9841 ggcaaaatca ggtttaaacc gttcgatact ggatcagggc tggtatgaaa tgcgccgcca
     9901 gcttgagtac aagcagctct ggagtggcgg tcaggtgctt gctgttccgc cagcgttgcg
     9961 cgtgctgtgg tcatacagcg aaagaaaatc gcctgtcaca aagtaaattc agatgccagg
    10021 tatgtggata tacagcgaac gccgatgtaa atggcgctcg taacatttta gcggcggggc
    10081 acgccgttct tgcctgtgga gagatggtgc agtcaggccg cccgttgaag caggaaccca
    10141 ccgaaatgat ttaggcgaca gcctgaacgt agcagggatc ctcgtccttc agggcgtgga
    10201 ggatgtcaat ttgcgctatg atacccatct gacctccttg tagcggatgg caaaagcata
    10261 tcaggaaacg ggcggacgaa attcttaaaa aaaaccgcta aagatagagc gttagtgaaa
    10321 ttgcaaagga gcaacaatga aaatttcccg ccttggagaa gcgcccgatt accgcttctc
    10381 gctggctaat gagcgtactt ttctggcgtg gatccgtacc gcactaggat ttctggcggc
    10441 gggggttggg cttgatcaac ttgcgccaga tttcgccacg cccgtcattc gtgaactgct
    10501 ggcgttgttg ttgtgtctgt tttccggtgg gttagcaatg tatggctacc tgcgctggtt
    10561 acgcaatgaa aaagcgatgc gtctgaaaga ggacctgccc tacaccaaca gcttattaat
    10621 cattagctta atattgatgg tcgtggcggt gatcgttatg ggactggtgt tgtatgccgg
    10681 atagccgcaa agccagacgc attgccgacc caggactgca gccggagcgc acatcactgg
    10741 cgtggtttcg taccatgctg ggctacggcg cgttgatggc gttggctatc aaacacaact
    10801 ggcaccaggc gggcatgtta ttctggattt cgattggcat tctcgccatc gtggcgctga
    10861 tcctctggca ctacacccgt aatcgcaatt taatggatgt cacgaatagc gatttttccc
    10921 aatttcacgt agtccgtgac aaatttttga tctccctcgc ggtgttatct ctcgcaatac
    10981 tgtttgctgt aacgcatata catcaactta tcgtatttat tgagagagtc gcatgacagg
    11041 aagtcaggtc attgatgctg aagaggacag gcataaactg gttgtagagt acaaggatgc
    11101 tctgcaacct gccgattttt atcacaattt caaacagcgg ggcatccgct ccgtacagct
    11161 cattccctat cttgaatttg atgaccgggg cgatctgacg gccgcttcgg tgacagcaga
    11221 gctgtggggg aaatttttaa ttgccctgtt cgagtgttgg gtgcgggcag atatcagtcg
    11281 tatctcgatt gagctttttg acgccacctt acaaaaatgg tgtggcggcg aaaacccgca
    11341 accgcgccgt gactgccagg cgtgcgactg gcatcgcctg tgcccgcacg ctcggcagga
    11401 gacgccagac agcgtgcttt gtgcgggcta tcaggcgttt tattcttact ccgcaccgca
    11461 tatgcgggtg atgcgtgatc tgataaagca gcatcgttct cccatggagc tgatgacgat
    11521 actgcggtga cgtgcgctta aacgggctgc ccctgatgcg acatccgcgt cgccagcggc
    11581 agccagcaca gcacgatcaa caatcccatt aaggtcatca ataaccccag gctaccctga
    11641 ccggtttgcg gcaacatcgc agagagcgac gccagcacgc cggaaccaat gttttgcaga
    11701 ccgccgacca gcgcgccagc cgtgcccgcc aggaagggga acggctccat cgcgccgctg
    11761 gtcgccagcg gaaacagcat cccggcaccg aaaaagaaca gcgcggcggg aacgagcagc
    11821 gtccagacat tcatcacgcc aaaccagtcg gggatccaca tcagcaagcc agccagcagg
    11881 cagcagataa ccgactgcca cattaacgtg gagaagcgtt tattgggacg tccggcaaac
    11941 catgcgccaa aaaacgctgc cggaatcggc agaataaaca aaatactgac cgtcatactg
    12001 ctcagcccta acaccgcgcc catcagcacg ccggagcagg cttcaaaggc ggcaatcccg
    12061 gccagaccgc caatcagcat cagcaaataa cagttaaaac cgctgttacc gaaaagcgtt
    12121 ttataactgg taagcaggcg cgtgcgcggt gcatcgaccg gacgcgtttc cggcatccag
    12181 cgggccatac tgaaggtcac accaacacaa agaaccaaca aaaagagata acaggcgcgc
    12241 cagttccaca tcgtatccag cagaccgccg attagcggtg cgagcaacgg actgacgaga
    12301 atccccatgt ttaacaggct gttagcatgg cacaactgtg tccgttcata taaatctcgc
    12361 ggcaaagtac gcgccattac gccgccaacg ccggtgccca tcccctgcat cgcgctggcg
    12421 gcaatcaata ccgtcaaact ggaggtcgtg accgcgacca gcgttgccag cataaaaatg
    12481 gacattccga cgaggatcac cggtcggcga cccacacggt cggaaatcgg gccataaaac
    12541 agctgtgaga caccgtaagt cagcagataa gcgcccatta cgctctgcac cgccccttca
    12601 cggacgttga gatcgcgcgc catatcggca atagctggaa tataaatggt ttgcgccatc
    12661 tgaccgacgg ccacgagtaa taccaacatc aataacaaat tgacgtttct ttgccttttc
    12721 attatcacgg atgcttttat aaaaataatt agaataagtc actacgcatt cccataaatg
    12781 cgggagagga atctatcaca ttgatgaatg ttagccagat atacgccagg aatggggaat
    12841 tgtttagcgg cagaatatgt aaacaaaagc ggcaataaat gttgccggga gggattacgt
    12901 gcgaactgta gccctctccc caaaggggcg agggggaaac caccgttccc tttcagaaaa
    12961 tgagctaaac attgcacggg acggtcccct cgcccctttg gggagagggc tagggtgagg
    13021 ggcacaggca gcgcaggtgc aaacattctg ctggtagaag aagcccccaa ctccaacccg
    13081 cacgctaaat ataaaagggg agcggttacc cgctcccctt tggtgcgact tgaatctgaa
    13141 ttacttcagg tatttcagaa cagcatcaag cagttgcagt gctgcaacaa tgagtttgag
    13201 gataagaatg gcgatatcca ccaggctcat acgcgtctcc tgtggttcag gagcactgcc
    13261 agctgacgta cctttccgct gcctgttgcc agctgttgtg cacgcggtat ctcaaggaga
    13321 aacgttgtgc ttgttgacat aacacagtgt gctcacgcgg ggttaaggcg ctgacggcac
    13381 cacccgtttc agccaggact tagtgcgccg ggtaacgatg cggcccccgc ataatacatt
    13441 gcgtacagtg atattataga agtttactgt ataaataaac agtaatattt ggacaaaacg
    13501 caaactgtga tcgatactca actatgccct aaatacgtca aaattcgtgc cgaaattgcg
    13561 cgttctgcgc ggaacacgta tactttcagt gttgacataa tacagtgtgc tttgcggtta
    13621 ccagctgcag gcggctgatg aaacctcgct ccggcggggt tttttgttac ctgcaattca
    13681 gtacaaaacg tgatcagccc ctcaattttc ccttgctgaa aaattttcta ttgtctcccc
    13741 tgtaaagctg tgcttgtata aatattgtta aacacaaaac caacaaggtc cccaatgact
    13801 acttccatgt tcaacgcaaa actactacca actgcgctat ccgtcgcagt ggtcgtcgtg
    13861 cgtgtggtgg tggtcgtcgg caatgcgccg tagggactgg aacaacacac gattccaaaa
    13921 ccccgccggc gcaaaccggg cggggttttt cgtttaagca cctcccggaa agtcggccca
    13981 gaagaaaagg actggagcat ggcaagttcg ggcacaacat cgacgcgtaa gcgctttacc
    14041 ggcgcagaat ttatcgttca tttcctggaa cagcagggca ttaagattgt gacgggcatt
    14101 ccgggcggtt ctatcctgcc tgtttacgat gccttaagcc aaagcacgca aatccgccat
    14161 attctggccc gtcatgaaca gggcgcggga tttatcgctc aggggatggc gcgcaccgac
    14221 ggtaaaccgg cggtctgtat ggcctgtagc ggaccgggtg cgactaacct ggtgaccgcc
    14281 attgccgatg cgcggctgga ctccatcccg ctgatttgca tcaccggtca ggttcccgcc
    14341 tcgatgatcg gcaccgacgc cttccaggaa gtggacacct acggcatctc tatccccatc
    14401 accaaacaca actatctggt cagacatatc gaagaactcc cgcaggtcat gagcgatgcc
    14461 ttccgcattg cgcaatcagg ccgcccaggc ccggtgtgga tagacattcc taaggatgtg
    14521 caaacggcag tttttgagat tgaaacacag cccgctatgg cagaaaaagc cgccgccccc
    14581 gcctttagcg aagaaagcat tcgtgacgca gcggcgatga ttaacgctgc caaacgcccg
    14641 gtgctttatc tgggcggcgg tgtgatcaat gcgcccgcac gggtgcgtga actggcggag
    14701 aaagcgcaac tgcctaccac catgacttta atggcgctgg gcatgttgcc aaaagcgcat
    14761 ccgttgtcgc tgggtatgct ggggatgcac ggcgtgcgca gcaccaacta tattttgcag
    14821 gaggcggatt tgttgatagt gctcggtgcg cgttttgatg accgggcgat tggcaaaacc
    14881 gagcagttct gtccgaatgc caaaattatt catgtcgata tcgatcgcgc agagctgggt
    14941 aaaatcaagc agccgcatgt ggcgattcag gcggatgttg atgacgtgct ggcgcaattg
    15001 atcccgcagg tggaagcgca accgcgtgca gagtggcacc agttggtagc ggatttgcag
    15061 cgcgaattcc cgtgtccaat cccgaaagcg tgcgatccat taagccatta cggcctgatc
    15121 aacgccgttg ccgcctgtgt cgatgacaat gcgattatca ccaccgatgt gggtcagcat
    15181 cagatgtgga ccgcgcaagc ttatccgctc aatcgcccac gccagtggct gacctccggt
    15241 gggctgggca cgatgggttt tggcctgcct gcggcgattg gcgcggcgct ggcgaacccg
    15301 gatcgcaaag tgttgtgttt ctccggcgac ggcagcctga tgatgaatat tcaggagatg
    15361 gcgaccgcca gtgaaaatca gctggatgtc aaaatcattc tgatgaacaa cgaagcgctg
    15421 gggctggtgc atcagcaaca gagtctgttc tacgagcaag gcgtttttgc cgccacctat
    15481 ccgggcaaaa tcaactttat gcagattgcc gccggattcg gcctcgaaac ctgtgatttg
    15541 aataacgaag ccgatccgca ggctgcattg caggaaatca tcaatcgccc tggcccggcg
    15601 ctgatccatg tgcgcattga tgccgaagaa aaagtttacc cgatggtgcc gccaggtgcg
    15661 gcgaatactg aaatggtggg ggaataagcc atgcaaaaca caactcataa caacgtaatt
    15721 ctggagctca ccgttcgcaa ccatccgggc gtaatgaccc acgtttgtgg cctttttgcc
    15781 cgccgcgctt ttaacgttga aggcattctt tgtctgccga ttcaggacag cgacaaaagc
    15841 catatctggc tactggtcaa tgacgaccag cgtctggagc agatgataag ccaaatcgat
    15901 aagctggaag atgtcgtgaa agtacagcgt aatcagtccg atccgacgat gtttaacaag
    15961 atcgcggtgt tttttcagta accgctcaag gcttgaacaa catcgcgctt atcgttaagg
    16021 taagcgcgtt tttttacccg ccaggacaag accatgatca ccgttgccct tatagacgat
    16081 cacctcatcg tccgctccgg ctttgcgcag ctgctggggc tggaacctga tttgcaggta
    16141 gttgccgagt ttggttcggg gcgcgaggcg ctggcggggc tgccggggcg cggtgtgcag
    16201 gtgtgtattt gcgatatctc catgcccgat atctccggtc tggagctgct aagccagctg
    16261 ccgaaaggta tggcgacgat tatgctctcc gttcacgaca gtcctgcgct ggttgagcag
    16321 gcgcttaacg cgggggcacg cggctttctt tccaaacgct gtagcccgga tgaactcatt
    16381 gctgcggtgc atacggttgc cacgggcggc tgttatctga cgccggatat agccattaaa
    16441 ctggcatccg gtcgtcagga cccgctaacc aaacgtgaac gccaggtggc ggaaaaactg
    16501 gcgcaaggaa tggcggtgaa agagattgcc gccgaactgg gcttgtcacc gaaaacggta
    16561 cacgtccatc gcgccaatct gatggaaaaa ctgggcgtca gtaacgacgt agagctggcg
    16621 cgccgcatgt ttgatggctg gtgatgaaga cgttgttctc ccgcttaatt accgttattg
    16681 cctgcttttt tatcttctct gccgcatggt tttgcctgtg gagtatcagc ctgcatctgg
    16741 ttgagcgccc tgatatggcg gtgctgttat ttccgtttgg tctgcgtctg gggctaatgc
    16801 tgcaatgccc gcgcggatac tggccggtgc tgctgggcgc ggagtggctg ctgatttact
    16861 ggctaacgca ggcggtcggt ttaacccatt ttccgttatt gatgatcggt agtttactga
    16921 cgttactgcc cgtagcgctg atctcgcgct atcgccatca gcgtgactgg cgcaccttgc
    16981 tgttacaggg ggcggcgtta acggcggcgg cgttgttgca gtcgctgccc tggctttggc
    17041 acggcaaaga gtcgtggaat gcgctgttgc tgactttaac tggcggcctg acgctggccc
    17101 cgatatgtct ggtgttctgg cactatctcg ccaataacac ctggctgccg ctcggcccgt
    17161 cactggtttc tcagccaatc aactggcgcg ggcgacatct ggtctggtac ttgctgctgt
    17221 ttgttatcag tctctggctc cagttgggat tgccggacga actgtcgcgc tttacgccat
    17281 tctgtctggc gctgccgatt atcgcgctgg cctggcacta tggttggcaa ggggcgctga
    17341 ttgcgacgtt gatgaacgcc atcgcgctga tcgccagtca aacctggcgc gatcatccgg
    17401 tggatttatt gctctcgctg ctggtgcaaa gtctgacagg gttgttgctt ggcgctggca
    17461 tccagcggtt gcgtgaactt aaccagtcgc tgcaaaagga actggcgcgc aatcagcatc
    17521 tggctgaacg gttgctggaa accgaagaga gcgtgcgccg tgatgtggcg cgtgagctgc
    17581 atgatgatat cggtcagacc atcactgcta ttcgtactca ggcgggcatt gttcagcggc
    17641 tggcggcaga taacgccagc gtgaagcaga gcgggcagct catcgaacaa ctatcgctgg
    17701 gcgtttacga cgcggtgcgc cgtttgttgg ggcggttacg tccgcgccag ttggatgatc
    17761 tcaccctgga gcaggccatc cgctcactga tgcgggaaat ggagctggaa gggcacggta
    17821 ttgtcagcca tctcgaatgg cgaatcgatg aatcagcgtt aagcgaaaac cagcgcgtga
    17881 cgctgtttcg tgtctgccag gaagggctga acaacattgt gaaacatgct gatgccagcg
    17941 cggtcaccct gcaaggctgg cagcaggatg aacggttgat gctggttatt gaagacgatg
    18001 gcagcggttt gccgccaggt tccgggcaac aaggttttgg cctcaccgga atgcgcgagc
    18061 gcgtaacggc gctgggtggc acattacaca tttcctgtct gcacggcacg cgtgtcagcg
    18121 tttctctacc tcaacgctat gtctaaggtt tgatgatgtt gccgtttctg aaagcgcctg
    18181 ccgatgcgcc attaatgact gataaatatg aaattgatgc ccgctatcgc tactggcgtc
    18241 ggcatattct gctgaccatc tggctgggtt acgcgctgtt ttacttcacg cggaaaagtt
    18301 ttaacgccgc cgtaccagaa atccttgcta acggcgtgct cagccgtagc gatatcggcc
    18361 tgttagcgac cctgttttac attacctatg gcgtgtcgaa gtttgtctcc ggcattgtca
    18421 gcgatcgctc aaatgcccgt tattttatgg ggatagggct tatcgccacg ggcattatca
    18481 acattctgtt tggcttctcg acgtcgctat gggcgtttgc cgtactctgg gtgctgaacg
    18541 cctttttcca gggctggggt tcaccggtgt gtgcgcgtct gttaacggcc tggtattcac
    18601 gtaccgagcg cggtggttgg tgggcattat ggaacacggc gcataacgtc ggcggcgcac
    18661 tcattcccat tgtgatggca gcggctgcac tacattacgg ctggcgtgcc gggatgatga
    18721 ttgctggttg tatggcgatc gtcgtgggaa tttttctctg ctggcggctg cgcgatcgcc
    18781 cgcaggcgtt aggtttaccg gcggtcggtg aatggcgaca cgacgcgctg gagatagctc
    18841 aacaacaaga aggggcaggg ctgacgcgta aagagatcct caccaaatat gtgttgctga
    18901 atccgtatat ctggctgctt tcgttttgct atgtgctggt ctatgtggtc cgggcggcga
    18961 tcaacgactg gggcaatttg tatatgtccg agacgctggg cgtcgatctg gtcacggcga
    19021 atacggcagt gacgatgttt gaactgggtg gatttatcgg tgcgctggta gcgggttggg
    19081 gctcggacaa attgtttaac ggcaaccgag ggccgatgaa tttgattttc gccgccggaa
    19141 ttttgctttc agtcggctcc ctgtggctga tgccatttgc cagctacgtg atgcaggcaa
    19201 cctgcttctt caccattggt ttttttgtct ttggcccaca gatgttaatc ggtatggcgg
    19261 cggcagagtg ttcccacaaa gaggcggcag gggcggcgac ggggtttgtc ggcttgtttg
    19321 cttatctggg ggcgtcgctt gctggttggc cgctggcgaa agtactcgat acctggcact
    19381 ggagcggatt ttttgtggtt atctctatcg ccgccgggat ttccgcactg ctgttactgc
    19441 cctttttgaa cgcccagaca ccgcgcgaag cgtgatgcat ctcacctttt cacttcatat
    19501 ccggcaaaac taagaaattt tccaggtttt gcctggacgc tatctcaggc ctgatttgct
    19561 gctgattttt acaatgcatg cctcacgcag gtattcattt caggagtaac ccatgctggc
    19621 tttcctaaac caggttcgca agccgaccct ggaccttccg ctcgaagtgc ggcgcaaaat
    19681 gtggttcaaa ccgttcatgc aatcctacct ggtggtcttt atcggctacc tgacgatgta
    19741 cctgattcgc aagaacttta acatcgcgca gaacgatatg atttcgacct acgggttgag
    19801 catgacgcag ctggggatga tcggcctggg tttctccatc acttatggcg tgggtaaaac
    19861 gttggtttca tactacgccg acggcaaaaa caccaaacaa ttcctgccgt tcatgctgat
    19921 cctgtctgcc atttgtatgc tgtgcttcag tgccagtatg ggcagcggct cggttagcct
    19981 gttcctgatg attgccttct acgccttaag cggctttttc cagagtaccg gcggttcgtg
    20041 cagttactcc accatcacca aatggacgcc gcgtcgtaaa cgcgggacat tcctcggttt
    20101 ctggaatatt tctcacaacc ttggcggtgc aggcgcagca ggtgtggcgc tgttcggggc
    20161 aaattacctg ttcgatggcc atgtcatcgg catgtttatc ttcccgtcga ttatcgcgct
    20221 gattgtcggt tttatcggcc tgcgttacgg cagcgactcc ccagaatctt atggcctcgg
    20281 caaagctgaa gaactgttcg gcgaggagat cagcgaagag gacaaagaga cagaatctac
    20341 cgatatgacc aagtggcaga tctttgttga gtatgtgctg aaaaacaaag tgatctggct
    20401 gctgtgcttc gccaacattt tcctctatgt ggtacgtatt ggtatcgacc agtggtcaac
    20461 cgtatacgcg ttccaggaac tgaaactctc taaagcggtg gcgattcagg gctttacgct
    20521 gtttgaagct ggtgcgctgg tcggtacgct gctgtggggc tggctctctg acctggcgaa
    20581 cggtcgccgt ggcctggtgg cctgcatcgc gctggcgctg attatcgcca cgctcggtgt
    20641 gtatcaacat gccagcaacg aatatatcta tctggcttct ctctttgcgt tgggtttcct
    20701 ggtctttggc ccgcaattgt tgattggtgt ggctgctgtt ggctttgtac ctaaaaaagc
    20761 gattggcgct gccgatggta ttaaaggcac ctttgcttac ctgattggtg acagctttgc
    20821 caagttaggt ctgggaatga ttgccgatgg gacgccggta ttcggcctta ccggctgggc
    20881 aggcaccttc gccgcacttg atatcgctgc gattggttgt atctgcctga tggcgatagt
    20941 ggcggtgatg gaagaacgca aaatccgccg cgagaaaaaa attcagcagt tgacagtggc
    21001 ataaacgtaa ctggtgactt ttgcccggca tgacgccggg cttttttatt attccgtgac
    21061 ttccagcgta gtgaaggcaa acttctcgcc atcaaatagc ccctgactgg ttagttttag
    21121 cgcggggatc actggcagag aaagaaacgc catctgaata aacggctcat cgggtaacgg
    21181 accgcattca cgggcggcgg ctttcaaggc gtcaatttgt tccgccagcg actgcgccgt
    21241 gtcggtgctc atcaggccgg caatgggcaa cggcagatga ctttggacct ggccgttacg
    21301 taccacgcac agcccgccgc catcctgaat cacctgatag accgccagcg ccatctcttc
    21361 ggcactgcga ccgatcacca caatattatg gctgtcatgg ctgaccgtcg cagccagcgc
    21421 accttcattc agtccaaagc cgccaagcaa accacaagcc ggagccagcc gttgcccgta
    21481 acgctcaagt acggcaataa agtacacatc atcgcgatca aaaccatttt cgctgtagac
    21541 gctggagtgg gagtgcgtaa tcaattcgtt atggatgacg tcaatgaccc gatagcgttt
    21601 tccgggcgta aattgcaggg caaagtcgct ggcggaaact ggctggcggg caatggtgtt
    21661 gccatatggc ggagcggatt gtgccagtct cgccgactct tccgcctgta aggtttgcgc
    21721 atcaatcggc tcgcctttca ccagtacctg ctgcaccgtg accttacgcg catcgctcaa
    21781 caggacgata tcggcctgct tgccgggtgc cagtaagccg aggtgattca gaccaaagtg
    21841 gcgcgccgtc gaccagctgg cgacgcgata tgccacatgc agcggcacat tgtattgttc
    21901 gatcaggcgg cgaattaagg catcgatgtg tccttcatgg gcgatctccc acgggttacg
    21961 gtcatcggta cagagcatgc attgcgggct gttaaattcg ttgatcaacg gtgccagcgc
    22021 gttgagattg cgggcagcgg acccttcgcg gatcatcaac gacatgccga gttgtaattt
    22081 ccggcgtcct tcttccagct gataactttc gtggcagttt tcaatacccg cagtaatata
    22141 ggcgttaagt tctttaccac ccaaacccgg gcagtgaccg tccagcgtca ggtggcgaaa
    22201 tgcatccagt ttatcgagca gcgcattctg cccgctaatt acgccagggt agtccatcat
    22261 ttctgcaagg ccggtaacct gcggatggtc ccgccaggcg agcatctgtt caagggtaaa
    22321 actggcaccg ttaacatcgc agccttccag ggcgggtacg caagagctga cctgtaagta
    22381 ctggttttgc cttgcctgtt cggcacagcg ggcaaaccag gcgaatccgg cttctcccat
    22441 cacgttgacg atttcatgag ggtcgcaaat aacggtcgtc aggccgcgcg gcagggtagc
    22501 ggtttcaaag gtgaccggcg tcatcatgct ggattcaata tgcaggtgag catcaataaa
    22561 ccctggcacc gccgttgcgc cgcgagcatc aatccgctgc aaagccggag catcagcgta
    22621 ttctgcgcca acaccggcaa tgtaacgtcc tttaatcaca attgggccgg aaatttctcc
    22681 gccattgatc aggtcgagaa tagagacatt atcaataata taatcggcaa cagcgtcgcc
    22741 acgggaaacg gctaacaatt
//
LOCUS       DAJTMK010000059        22726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTMK010000059 DAJTMK010000000
VERSION     DAJTMK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22726
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.060"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="OO135_001888"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="OO135_001888"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5397755.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO135_001889"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="OO135_001889"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5397756.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSTRRRTRRPRKRAPRREGTA"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO135_001890"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="OO135_001890"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5397757.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO135_001891"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="OO135_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5397758.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO135_001892"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="OO135_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5397759.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO135_001893"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="OO135_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5397760.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="OO135_001894"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="OO135_001894"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5397761.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEYHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7613..10129
                     /gene="mrcB"
                     /locus_tag="OO135_001895"
     CDS             7613..10129
                     /gene="mrcB"
                     /locus_tag="OO135_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5397762.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLVNLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGVKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10349..12538
                     /gene="fhuA"
                     /locus_tag="OO135_001896"
     CDS             10349..12538
                     /gene="fhuA"
                     /locus_tag="OO135_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5397763.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGYYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12589..13386
                     /gene="fhuC"
                     /locus_tag="OO135_001897"
     CDS             12589..13386
                     /gene="fhuC"
                     /locus_tag="OO135_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5397764.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13386..14276
                     /gene="fhuD"
                     /locus_tag="OO135_001898"
     CDS             13386..14276
                     /gene="fhuD"
                     /locus_tag="OO135_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5397765.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14273..16255
                     /gene="fhuB"
                     /locus_tag="OO135_001899"
     CDS             14273..16255
                     /gene="fhuB"
                     /locus_tag="OO135_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5397766.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16290..17570)
                     /gene="hemL"
                     /locus_tag="OO135_001900"
     CDS             complement(16290..17570)
                     /gene="hemL"
                     /locus_tag="OO135_001900"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5397767.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVLPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17795..19216
                     /gene="clcA"
                     /locus_tag="OO135_001901"
     CDS             17795..19216
                     /gene="clcA"
                     /locus_tag="OO135_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5397768.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MELDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19240..19305
                     /locus_tag="OO135_001902"
     CDS             19240..19305
                     /locus_tag="OO135_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397769.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19298..19642
                     /gene="erpA"
                     /locus_tag="OO135_001903"
     CDS             19298..19642
                     /gene="erpA"
                     /locus_tag="OO135_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5397770.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19689..20312)
                     /locus_tag="OO135_001904"
     CDS             complement(19689..20312)
                     /locus_tag="OO135_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5397771.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVHQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20350..21149)
                     /gene="btuF"
                     /locus_tag="OO135_001905"
                     /pseudo
     CDS             complement(20350..21149)
                     /gene="btuF"
                     /locus_tag="OO135_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21142..21840)
                     /gene="mtnN"
                     /locus_tag="OO135_001906"
     CDS             complement(21142..21840)
                     /gene="mtnN"
                     /locus_tag="OO135_001906"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5397772.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDANV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     ILMVESLVQKLAHG"
     gene            21924..>22726
                     /gene="dgt"
                     /locus_tag="OO135_001907"
     CDS             21924..>22726
                     /gene="dgt"
                     /locus_tag="OO135_001907"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5397773.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAA"
BASE COUNT         5163 a         5751 c         6338 g         5474 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaattcgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgatcctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgagcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 agtgcggtaa aacgaagaaa cggcaagaat gtggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg cggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacggcga
      661 cgcgttgacg gctcttcatc caactcgtta agcatccctt tttgatctgg tggcgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcgttacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttctt gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcatgccgcc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt tgcaacttcg ataatctccg ggccaaacat cacatgagcc
     1681 agacggaaac ggcgacccac aaggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttattga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcgtccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2341 gctggtacag cgagatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgccgc
     2521 gcagctggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgacaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa
     3841 ccgcacgctg gccttcagcc gctacgttca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttctg
     4021 cgccatattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttagt tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccacgcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagagaattc cattgcgaca atccttgtta
     4381 ttgcattagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacctggcg gcgggattgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggcggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctcggcg ctcacttcgc ctaaaaatgc cagagtcaga tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccag tggataatct gttcgcggat
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggagga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggccgaat acccgcctgg aagtagttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggag ttgagcggtg ttggattggt
     5341 gatccttgat gaatatcatg agcgtagctt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgcta attatgtcgg ctacgctgga
     5461 caacgaccgc ttgcagcaaa tgctgccaga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgatgccgt
     5581 tgcggtagcc accgctgaaa tgctgcgtca ggaaagcgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gtgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 attgctctgc ccgctgtatg gcgcgttgtc gctgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gttttgatcc
     5881 gcgcacgggg ctaacgcgac tgattactca acgcgttagc caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatctgcctg catctaatcg ccaaagaaca
     6001 agcagaacgc gctgccgcgc aaagtgaacc ggaaatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 aggtgaacgg cttagtgcgc aggggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttagcg cgaagaacga cgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgtgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcgtcgc cgtgggcaag atggacgcta tcaactggca aacggcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctgccg gtcgatattg atgagttagt
     6661 acaacgctgc ccgcagctgg tacagcagtc tgacactgtg gagtgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctgcaaat cggtctgctg acggtgaaag tgcagccgct
     6781 ggcgaaaccc tcggaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tattggcgac
     6961 gctggaaacg tggctgctgc cacatatgac tggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaagag atgtttggcg aggccaccaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctggag ttgctttcac ctgcccaaag
     7261 gccattacaa atcacacgag atttgagcgc cttctggaaa ggagcgtacc gtgaggtgca
     7321 aaaagagatg aaagggcgtt atcccaaaca tgtctggccg gacgacccgg caaatactgc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct aaccctctcc
     7501 ctcaagggag aggggaccga tcgaacacaa attttgagag atatcttctt ctgtcttgta
     7561 acagaagaac agaaaatcgg gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg
     7621 gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt
     7681 aagccgtcgt cgttacgaag atgacgatga ttacgacgat tatgatgact atgaggatga
     7741 agaaccgatg ccgcgcaaag gtaagggcaa agggcgtaag cctcgtggca aacgcggctg
     7801 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7861 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcctgcggc
     7921 agtttatggc cgaatggtca atcttgagcc agatatgacc atcagcaaga acgagatggt
     7981 gaagctgctg gaggcgactc agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     8041 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8101 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8161 tatggagaac aaccgtcagt tcggcttttt ccgtcttgat ccgcgtctga tcaccatgat
     8221 ctcctcgcca aacggtgagc aacgtctgtt tgtgccgcgc agtggtttcc ctgatttgct
     8281 ggtggatact ttgctggcga cagaagaccg ccatttctac gagcatgatg ggatcagcct
     8341 ctactcaatt gggcgtgcgg tgctggtaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8401 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gctcttactg
     8461 gcgtaaagcg aacgaagcct acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8521 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8581 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8641 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8701 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8761 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgcgg
     8821 tggggtgatc tctcctcagc cagcctttat gcaactggtg cgtcaggagc tgcaggcaaa
     8881 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8941 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     9001 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9061 tgcgatggtg ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9121 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9181 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9241 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9301 ggtggatgcg ttgacccgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9361 gctgcctgca gttacggata cctggatcaa actgggcgta ccgaaagatc agctgcatcc
     9421 ggttccggcg atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcgtt
     9481 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9541 ggaagatggc aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9601 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9661 gcttggggtg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9721 cgtagatact tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggtcg
     9781 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9841 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9901 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9961 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
    10021 caatccgttt gatcagtctt ctcagccgca acagcaacct gctcagcaag agcagaaaga
    10081 cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac atctaagcgt
    10141 gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag atattaaccc
    10201 tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg taccgcttgc
    10261 gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc gtttacatta
    10321 tcattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc agccaaaaca
    10381 ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt ctgtttatgc
    10441 acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac ctgcgccgca
    10501 agaaagcgca tgggggcctg ctgcaactat tgcggcgaag cactctgcta ctgcgactaa
    10561 aacggataca ccaattgaaa aaacgccgca gtctatttcg gttgtgacaa atgaagagat
    10621 gcagatgcat caatttcagt ctgtaaaaga agcattaggt tatacacctg gcgttacggt
    10681 tagcagtcgt ggtgcttcta atacatatga ttttgttatc attcgtggtt tctcatctgt
    10741 tggtctgaat caaaataatt accttgatgg actaaaactt cagggcaact tttataacga
    10801 tgctgtgatt gatccttaca tgctcgagag ggttgaactg atgcgtgggc cgacgtctgt
    10861 tctctatgga aaaagtaatc ctggtgggat tatttctatg gtgagtaagc gtccgaccac
    10921 tgaacccctg aaagaaattc agtttaaaat ggggacggat aatctgtttc agaccggatt
    10981 cgatttcagt gatgcactgg atgataacgg tgagttctct taccgtttga cgggcctcgc
    11041 tcgctcaaca aacgaacagc agaaaaactc tgaatctcag cgttatacca ttgctccatc
    11101 attctcatgg cgtccagacg acaaaaccaa ttttactttc ctgtcctatt tccagaatga
    11161 accagaaacg ggttattacg gttggttgcc gaaagagggg accgttgagc cattgcctaa
    11221 tggtaagcgt ctaccgactg acttcaatga aggtgcatcg aataatacat actcccgtaa
    11281 ccagaaaatg gtgggatata gttttgaaca tggttttaat gacaccttca ccgtgcgtca
    11341 gaatctgcgt ttcagtgaaa tgaaaacctc acagaaaagt gtttatggca cagggattgc
    11401 tggtgatggt cataccctaa accgcgggac agtagtggat aatgagcgtc tacaaaactt
    11461 tagcgttgat acccaacttg aaagtaagtt tgctacaggt gacgtcgagc atactttgct
    11521 gacaggggta gacttcatgc ggatgcgtaa tgatatcaat gccagctttg gatccgcacc
    11581 atccatcgat ctttataaca aatatcaccc tgaatacttt gcatttggta gcgcagagcc
    11641 ataccagatg aatgaaagca aacaaacagg tatttatgtt caggatcagg cggaatggaa
    11701 taaatgggta ttcactctgg ggggacgcta cgattggtct aagcaagcga ccactgttcg
    11761 tgaaaactct tatacgccga ctgaaggtta tattgagcgc aatgatcatc agttcacctg
    11821 gcgcggtggt gtaaattact tattcgataa tggtatttca ccttacttta gctatagcca
    11881 gtcctttgaa ccgagtgctt tcgatctgtg gagcaaccca cgcatttcct ataagccatc
    11941 gaaaggtgaa cagtatgaag ctggcgtaaa atatgttccg aatgatatgc cggtcgttgt
    12001 tacgggcgca gtctatcaat tgacgaaaac aaataacctg acagcagacc caacaaaccc
    12061 attagcgcaa gtcccagcag gtgagattcg cgctcgtggt gtggaacttg aagcaaaagc
    12121 ggcgttaaat gccaatatta acttgacggc ttcttatacc tacaccgatg cggaatacac
    12181 caaagacacc aatctcaaag gtaaaactcc agaacaagta ccggagcata tggcatctct
    12241 ctgggggtat tataccttca atgaagggcc gctttctggt ttaacattgg gaacaggtgg
    12301 tcgttttatt ggttccagct atggtgatcc ggcaaacact tttaaagtgg gtagcgcagc
    12361 tgtaatggat gctgttgtaa aatatgatct ggcacgcttt ggtatggcgg gatccagcct
    12421 tgctgtgaac gtcaacaatt tgctcgatcg tgagtatgtt gccagttgct tccagactta
    12481 tggctgcttc tggggcgcag aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt
    12541 tctcttttgg ggcacggatt tccgtgccca tttcacaagt tggctgttat gcaggaatac
    12601 acgaatcatt ccgataccac ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc
    12661 acgcttttgc atccgctgtc gttaaccttt cctgccggga aagtgaccgg tctgattggt
    12721 cacaacggtt ctggtaaatc cactctgctc aaaatgcttg gccgtcatca gcctccgtca
    12781 gaaggggaga ttcttcttga tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc
    12841 cgcaaagtgg cttatttgcc gcagcagctt cctccggcag aagggatgac cgtgcgtgaa
    12901 ctggtggcga ttggtcgtta cccgtggcat ggcgcgctgg ggcgctttgg ggcggcagat
    12961 cgcgaaaaag tcgaggaagc tatctcgctg gttggcttaa aaccgctggc gcatcggctg
    13021 gttgatagtc tctctggcgg cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag
    13081 gatagccgtt gtctgttgct cgacgaaccg acctcggcgc tggatatcgc ccaccaggtt
    13141 gatgtgctgg cgctggtgca ccgtttaagt caggagcgtg gcctgacggt cattgccgtg
    13201 ttgcacgata tcaatatggc ggcacgctac tgtgattatc tggtcgccct gcgcggtggt
    13261 gaaatgattg ctcagggaac gcctgcggaa attatgcgcg gcgataccct cgaaatgatt
    13321 tatggcatcc cgatgggtat tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt
    13381 tattgatgag cggcttacct cttatttcgc gccgtcgact gttaacggcg atggcgcttt
    13441 ctccgttgtt atggcagatg aataccgccc acgcggcggc tattgatccc aatcgtattg
    13501 tggcgctgga gtggttgccg gtggaattac tgctggcgct cggcatcgtg ccttacggcg
    13561 tggcggatac catcaactat cgcctgtggg tcagcgaacc accattgccg gactcagtga
    13621 tcgacgtcgg tttgcgcaca gaacctaacc ttgaactgct gaccgaaatg aaaccgtcgt
    13681 ttatggtctg gtcggcagga tatggccctt cagcagaaat gctggcacgt attgcgccgg
    13741 ggcgtggatt taacttcagc gacggcaaac agccgctggc gatggcgcgt aaatcactga
    13801 cggaaatggc agatttactt aacctgcaaa gcgcagcaga aacgcattta gcgcaatatg
    13861 aagactttat ccgcagcatg aaaccccgct ttgtgaagcg tggtgcacgc ccgttattgc
    13921 tgacgacgct tatcgatccg cgccatatgt tggtcttcgg tccaaacagc ttgttccagg
    13981 aaattcttga tgagtacggc atcccaaatg cctggcaagg ggaaaccaac ttctggggca
    14041 gtaccgccgt cagtatcgat cgtctggcag cgtataaaga cgttgatgtg ctctgttttg
    14101 atcacgacaa cagcaaagac atggatgcgc taatggcaac cccgctgtgg caggccatgc
    14161 cgtttgtccg cgccggacgc tttcagcgcg tacccgcagt ctggttttat ggtgcgacgc
    14221 tctcggcaat gcactttgtg cgcgttctgg ataacgccat cggaggtaaa gcgtgagtaa
    14281 acgaattgcg cttttcccgg cgttattgct ggcgctgctg gtgattgtcg ctacggcgct
    14341 gacctggatg aacttttcgc aggcgctgcc gcgtagccag tgggcgcagg ccgcctggtc
    14401 gccggatatt gacgtcatcg agcagatgat ttttcactac agcttgctgc cgcgtctggc
    14461 gatttcgctg ctggtgggcg caggcctggg gctggtgggc gtgctgtttc agcaagtgct
    14521 gcgtaatccg ctggcggaac cgacgacact tggcgttgct acaggcgcgc aactggggat
    14581 taccgtcact acgctctggg cgatcccagg ggcgatggcg agtcagtttg ctgcgctggc
    14641 gggcgcttgc gttgttggct taatcgtctt tggcgtcgcg tgggggaaac ggctttcgcc
    14701 ggtaacgctg atcctcgcgg ggctggtagt gagcctttat tgtggggcaa tcaatcagtt
    14761 actggttatc ttccaccatg accaactgca aagcatgttc ctgtggagca ccggaacgct
    14821 gacgcaaacc gactggggcg gtgttgagcg tttatggccg cagttgctgg gcggtgtgat
    14881 gctgacgttg ctgctacttc gcccgttaac cctgatgggg cttgatgatg gcgtggcgcg
    14941 caatctcggg ctggccttgt cgctcgcgcg tctggcggcg ctgtcgctgg cgattgtcat
    15001 cagtgcgctg ctggtgaacg cggtggggat tatcggcttt atcgggttgt ttgcgccgtt
    15061 gctggcaaaa atgctggggg cgcggcgtct gctgccgcga ctgatgctgg cgtcgttgat
    15121 tggtgcgctg atcctctggc tttccgatca aatcatcctc tggctgactc gcgtgtggat
    15181 ggaagtgtcc accggttcgg tcactgcgtt gatcggtgcg ccgctgctac tgtggctgtt
    15241 gccgcgttta cgcagcatta gcgcgccgga tatgaaggtc aacgatcgtg tcgcggctga
    15301 acgccaacat gtgctggcgt ttgccctcgc gggcggcgtg ctgctgttga tggctgtggt
    15361 ggtggcgctg tcgtttggtc gtgatgcgca cggctggatg tgggcgagcg gggcgttgct
    15421 cgatgattta atgccctggc gctggccgcg aattatggcg gcgctatttg cgggcgtcat
    15481 gctggcggtg gcgggctgta ttattcagcg actgaccggc aacccgatgg caagcccgga
    15541 agtgctgggg attagctccg gcgcagcgtt tggcgtggta ctgatgctgt ttctggtacc
    15601 gggtaatgct tttggctggc tgttacccgc gggaagtatc ggtgccgcgg tgacgctgtt
    15661 gattattatg atcgccgccg ggcgcggtgg attttcccca caccgcatgt tactggcggg
    15721 gatggcgtta agtaccgcat tcaccatgct tttaatgatg ttgcaggcga gtggtgaccc
    15781 gcgaatggcg caagtgctca cctggatctc tggttcgacc tacaacgcca ccgatgcgca
    15841 ggtctggcgt accggaattg tgatggtgat tttgctggcg attacaccgc tgtaccgccg
    15901 ctggctgacc attttacctc tgggcggtga taccgcacga gctgtgggaa tggcgctgac
    15961 gccgacgcga attgcgctgc tgctgttagc ggcttgcctg acggcgaccg cgacgatgac
    16021 tattggaccg ttgagttttg ttggtttaat ggcaccgcat attgcgcgga tgatgggttt
    16081 tcgacggacg atgccacaca tcgtaatttc ggcgctggtg ggtggtttac tgctggtatt
    16141 cgctgactgg tgtgggcgga tggtgctgtt tccattccag atcccggcgg ggctgctgtc
    16201 gacctttatc ggcgcgccat attttatcta tttgttgaga aagcagagcc gttaattgag
    16261 tgcctcgccc ggtgggcgag gcaaaattat cacaacttcg caaatacccg acgtgcagca
    16321 tcgatggtgt tattgatatc ttccatgctg tgcgccacag acataaagcc cgcttcaaac
    16381 gctgacggtg ccaggtaaac accttcgtcc agcatcatat ggaagaaacg cttaaagcgt
    16441 tccacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaaa
    16501 ataccgaaca tgccgccaac gtggttaacg accagcggag ttccggcttc ttctgccgct
    16561 tccagcagac cttctgccag acgtgttgtc agctcatcca gcgtttcgtg aacgcccggc
    16621 tgcgcgactt cattcagaca ggcgaaaccc gctgccatcg caatcgggtt accggaaagc
    16681 gtacccgcct gatagaccgg acccgtcggg gccagcgcat ccattacatc acgacgacca
    16741 ccgaatgcgc ctaccggcat tccaccgccg atgattttgc cgaggcaggt taaatctggc
    16801 actacgccgt aataatcctg tgcgccagcc agtgccacgc ggaagccggt catcacttca
    16861 tcgatgatca gcaacgcgcc aaattcgtcg cacagcgcac gcagacctgg caggaactct
    16921 ggctgcggca gaacacagtt catattgcct gccaccggct cgacgataat acaggcaatc
    16981 tcttgcgggt attgctcaaa cgcggcgcgt acagaagcca gatcgttata agtacaggtt
    17041 aaggtatgtt tggcgaaatc tgccggaaca cccggcgagt ttggctggcc taacgtgagt
    17101 gcgccagaac cggctttcac cagcaggcag tcagcgtgac cgtggtaaca accttcaaat
    17161 ttaatgattt tgtcgcgacc ggtaaaacca cgggccaggc ggatggcgct catggtcgct
    17221 tcagtgccgg agttcaccat gcgcaccata tccatggtcg ggaccagttc ggtcaccagt
    17281 tgcgccattt tcacttccat ttcggtcggt gcaccaaagc ttaaaccacg ctcggcggct
    17341 tcaatcacgg cattgcggat agccggatgg ttatggccca gcaccatcgg cccccaggaa
    17401 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    17461 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacaccg
    17521 ccagggagca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    17581 tgattcgtag aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc
    17641 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    17701 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    17761 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    17821 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    17881 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg tcgggctggc
    17941 agcggttgct tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt
    18001 acatactgct gataattatc cgcttctgtt aaccgtcgct tttctctgtt cggcggtgct
    18061 ggcgatgttc ggctactttt tggtgcgcaa atacgcgccg gaagcaggtg gttcggggat
    18121 cccggaaatt gaaggggcgc tggaagacca acgtcccgtt cgctggtggc gtgtgttgcc
    18181 ggtgaaattc tttggcgggc tggggacact cggcggaggc atggtgctgg ggcgcgaagg
    18241 gccaaccgtg cagatcggcg gcaatatcgg tcgcatggaa ctcgatattt tccgcctgaa
    18301 aggtgatgaa gctcgccata cgctgctggc aaccggtgct gctgcggggc tggctgcggc
    18361 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    18421 ctatacgtta atttcgatta aagcggtatt tattggcgtc attatgtcga ccattatgta
    18481 ccgcattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct
    18541 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    18601 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggcg gcaatattac
    18661 caaatgggtg ctaatgggcg gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    18721 accagcaacg tcgggcggcg gttttaacct gattcctatc gctaccgcag ggaatttcag
    18781 catgggaatg ctggtgttta tcttcgtcgc gcgggtcatt accaccttac tgtgcttctc
    18841 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    18901 cgctttcgga atggttgtcg ttgagctgtt tccgcaatat caccttgagg cggggacttt
    18961 tgctattgcc ggaatggggg cgttactggc ggcatctatt cgcgcgccgt taacggggat
    19021 cattttggtt ctggagatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    19081 tcttggcgca acactattag cgcaatttac cggcgggaaa ccgctatatt cggcgattct
    19141 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    19201 cagcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    19261 gaatttgccc aattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    19321 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    19381 cccgaatctg aaattacgcg tgtatatcac cggtggcggt tgcagcggct tccagtatgg
    19441 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    19501 cctggtggtt gatccgatga gcctgcaata tctggtcggt ggttccgttg actataccga
    19561 aggactggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    19621 cggttcttcc tttagcatct aatctgttgt tcatgtgccg gataaactat ccggcacatc
    19681 ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    19741 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    19801 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    19861 gcgtagattt ctgtacgtaa aatcatgggg atttcgcggg ccagtacatc acgaatgatc
    19921 ccgccgccaa cgccagtaat gacgcccata caaaccgcga ttaacggacc ggcttccgca
    19981 ttaaaggctt tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    20041 ggcaacatcc attttggtaa gcgtcttggc tggtgcacca gcacgatggt cagcatgctg
    20101 gttaccattg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    20161 agcgccatgt cgcgaattgt cccgccgcct acagcggtaa ccacgcccag taccagaaca
    20221 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cggagatggc aaataccgct
    20281 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctaaaaa
    20341 ggtcaggcgc taatctacct gtgaaagcgc attacagagc tgttgtgcag cgaggataat
    20401 acgtgggctt gcacgtcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt
    20461 tcaccccagt attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc
    20521 tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg gccaaggaac ccggctgtct
    20581 ttaaagatgt tttctccgcc acaaacttcg agtacctggt tttgaatcga ctcttttcca
    20641 ctggtaaatg gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca
    20701 tattgcgctt tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggcctta
    20761 tccggttgcg gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt
    20821 gtcgcatcga cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg
    20881 gcattacctc cacgccaggc aatcaccaga tcgggtttca gcgcgacaat gcgttccaga
    20941 ttcatcccct gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa
    21001 tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg caagttcagt gttggcggga
    21061 gaaagcgtga tgacgcgcgg tgccgcgttg agccacagtg gcgcgagaaa agacagggcg
    21121 accagcgccc tgaacagtga cttagccatg tgccagtttc tgcaccagtg actcaaccat
    21181 caggatggac tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg
    21241 atcggccacg tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac
    21301 atgggcgatt gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat
    21361 tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac
    21421 agcgttaaga ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc
    21481 tttaaagcct gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatt
    21541 cgcgtcgtga taacgtgctt cgtccgagac aacgatatcg cccactttca acgttggtgc
    21601 caggccaccg gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcaa
    21661 agtggcaccc agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt
    21721 tccattcagt tggccggtat agatttcgca accgccgaga ctgatagttt gacgattttc
    21781 gattttgtca cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat
    21841 agatttactc gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc
    21901 gtatttctca cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc
    21961 atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg
    22021 agagcgatcg cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc
    22081 aggtttttcc actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag
    22141 tccagcaggt ggggcgctac atcgccaaag aaattctaag ccgtatgaaa gagcttaaat
    22201 tactggaagc atacggcctg gatgaactga ccggcccctt tgaaagcatt gttgagatgt
    22261 catgcctgat gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa
    22321 atgattggtt tcgccagcgt ttgcacccgg aagatgccga aagccagcct ctgactgacg
    22381 atcgctgcag cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagctgc
    22441 ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg
    22501 tgcatacatt gatgaggatg aatctcacct gggcacaggt tggcggtatt ttaaaatata
    22561 cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc
    22621 cgggttatta tctttctgaa gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg
    22681 cgctttacag tcgttttcca ttaacgtgga ttatggaagc agccga
//
LOCUS       DAJTMK010000060        22359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTMK010000060 DAJTMK010000000
VERSION     DAJTMK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22359
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.061"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1298
                     /locus_tag="OO135_001908"
     CDS             <1..1298
                     /locus_tag="OO135_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5397774.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRLLTQDEIDVRIKRFWRYLDRTLPGAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEITPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCPQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLPDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="OO135_001909"
                     /pseudo
     CDS             1270..2133
                     /locus_tag="OO135_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
     gene            complement(2347..3129)
                     /locus_tag="OO135_001910"
     CDS             complement(2347..3129)
                     /locus_tag="OO135_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5397775.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRVAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="OO135_001911"
     CDS             complement(3126..4199)
                     /locus_tag="OO135_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5397776.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGNLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="OO135_001912"
     CDS             complement(4321..4482)
                     /locus_tag="OO135_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5397777.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO135_001913"
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO135_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5397778.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTEQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO135_001914"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO135_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5397779.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7269..7964)
                     /gene="yjjG"
                     /locus_tag="OO135_001915"
     CDS             complement(7269..7964)
                     /gene="yjjG"
                     /locus_tag="OO135_001915"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5397780.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKHRLPPPR"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO135_001916"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO135_001916"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5397781.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO135_001917"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO135_001917"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5397782.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="OO135_001918"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="OO135_001918"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5397783.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLNAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="OO135_001919"
     tRNA            10209..10295
                     /locus_tag="OO135_001919"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            10324..10410
                     /locus_tag="OO135_001920"
     tRNA            10324..10410
                     /locus_tag="OO135_001920"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10358..10360,aa:Leu,seq:cag)
     gene            10445..10531
                     /locus_tag="OO135_001921"
     tRNA            10445..10531
                     /locus_tag="OO135_001921"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10479..10481,aa:Leu,seq:cag)
     gene            complement(10570..10806)
                     /locus_tag="OO135_001922"
     CDS             complement(10570..10806)
                     /locus_tag="OO135_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5397784.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10947..11735
                     /gene="fhuF"
                     /locus_tag="OO135_001923"
     CDS             10947..11735
                     /gene="fhuF"
                     /locus_tag="OO135_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5397785.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11773..12449)
                     /gene="bglJ"
                     /locus_tag="OO135_001924"
                     /pseudo
     CDS             complement(11773..12449)
                     /gene="bglJ"
                     /locus_tag="OO135_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12407..13132)
                     /locus_tag="OO135_001925"
     CDS             complement(12407..13132)
                     /locus_tag="OO135_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5397786.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13751..14521
                     /locus_tag="OO135_001926"
     CDS             13751..14521
                     /locus_tag="OO135_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5397787.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14512..14985
                     /locus_tag="OO135_001927"
     CDS             14512..14985
                     /locus_tag="OO135_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5397788.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            15093..15632
                     /gene="dnaT"
                     /locus_tag="OO135_001928"
     CDS             15093..15632
                     /gene="dnaT"
                     /locus_tag="OO135_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5397789.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15635..16372
                     /gene="dnaC"
                     /locus_tag="OO135_001929"
     CDS             15635..16372
                     /gene="dnaC"
                     /locus_tag="OO135_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5397790.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16421..16915
                     /locus_tag="OO135_001930"
     CDS             16421..16915
                     /locus_tag="OO135_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5397791.1"
                     /translation="MMKTVKHLLCCAIAASTLIPTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            17169..19460
                     /gene="opgB"
                     /locus_tag="OO135_001931"
     CDS             17169..19460
                     /gene="opgB"
                     /locus_tag="OO135_001931"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5397792.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19599..20621)
                     /gene="lgoD"
                     /locus_tag="OO135_001932"
     CDS             complement(19599..20621)
                     /gene="lgoD"
                     /locus_tag="OO135_001932"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5397793.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20760..21674
                     /locus_tag="OO135_001933"
     CDS             20760..21674
                     /locus_tag="OO135_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5397794.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLESISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21889..>22359
                     /locus_tag="OO135_001934"
     CDS             21889..>22359
                     /locus_tag="OO135_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5397795.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         5516 a         5847 c         5536 g         5460 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga cttctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacaga accctgccag gcgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcacccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgcccgca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgccg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgaaaaaac
     1381 tgtcacaatc cgtggacgat ggaacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcagaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 atagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tatggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtgtc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccacgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcgggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agattgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttgcgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctgg ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct aaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatctg tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggctgtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctgaatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgagacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gccggatgcg gcgtaaacgc cttatccgac ctacaatacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaaaaac cacgttgata ttgctcgcac
    10321 tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac
    10381 gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa agtaagatga
    10441 ttgtgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga
    10501 cgtgggggtt caagtccccc ccctcgcacc aattatcttt acttcctttc ttgtttcttc
    10561 cttgattttt catccctgat tcaaattcat tatcatcagc atgatggcag caataagcgc
    10621 caggcacgat ggtagcaaaa cgtaatgccg taactgtttg tgatacagca tcaccggcgt
    10681 caatactaat cccatcagaa tgacaatccg ccactgatcc ggcgataaat ccgcatacat
    10741 cagccctgca atcagcaatc ccggcagcaa cactccccag ccactgccca gcgtacgttg
    10801 caacatggtt tcatctcttt cattgataat gataaccaat atcatatgat aatttttatc
    10861 atttgcaagc cagagaaatc ccttgctatc gggtaaacct atcgctagga ttagcaatca
    10921 ttatcattta gattactatc ccgattatgg cctatcgttc cgcaccgctc tatgaagata
    10981 tcatctggcg aacgcatctc cagccgcagg atgctgggct tgcacaagcc gtacgtgcga
    11041 cgatcgccaa acatcgtgaa catttgctcg agtttatccg cctggatgaa cccgctccgc
    11101 ttaacgccat gacgctggcg caatggtcat caccgaatgc gctaagttct ctactcgcgg
    11161 tctattccga tcatatctat cgcaaccaac caacgatgat ccgcgagaac aaaccgctga
    11221 tctcactatg ggcacaatgg tatatcggcc tgatggtgcc accattaatg ctggcgctac
    11281 tgacgcagga aaaggcatta gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa
    11341 ccggacgcgt cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt
    11401 cgccgcagca gcgaatggaa acgttaatca gccaggcgct ggttccggtt gtgcaggcac
    11461 tagaagcgac cggagagatc aacggcaaac ttatctggag taataccggt tatttgatca
    11521 actggtatct cactgagatg aagcaactgc tcggcgaggc gacggttgaa tcgctgcgcc
    11581 atgccctctt ttttgagaaa acgctcacga acggtgaaga taatccactc tggcgtaccg
    11641 tcgtgctgcg cgatggcctg ctggtgcgcc gaacctgctg ccagcgttat cgcttaccgg
    11701 atgtgcagca atgtggcgat tgtacgctga aatgaaagca ctgccgggga agtaaacccg
    11761 gcatcatgcg gattaatagg gatgcaacac attacttgct tcgcaatgcc gcatacataa
    11821 cagaatatct gcggcctcca acaaccctgc atcagaactg acgcccagtt tcgacatcac
    11881 attaaattta tgtgcacgga tcgttttgat attgcgttta agctgctcgg caatttgtgt
    11941 cattgagtag ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc ttagcgtccg
    12001 gctttggtta atgtaccact gattgttcag tcggtcggtc gcctgacgta caccatttaa
    12061 tgacaaaaag agttcctgat gaaaaatctc cagcgttgac gctttactta atacaccgtc
    12121 cagcggtgat ggcgacaatg aaccaatcag ccgagcttca atatcatcat ccgcaataac
    12181 taaacgccgg gtacgcggaa acttaatcgc cagttcagtc aggcaagata atccctctct
    12241 gcgctcactt ctcatggcag aaaaagaaaa atgaccgccg caaatgagat ccgcgacatg
    12301 gcatcctgaa atgactcctg actggtatag gtatggagct tataagggtt acccgcaaac
    12361 tttctgaata aactctcaat accgatactg ctcatgacgc atttttctat gagtgcgaca
    12421 tttctcttct taattcggct gtgttccatt ctgcggcatc tccataaaca gaaggcaagt
    12481 taagttctct tgcaccctgc gcgctattta tccatgcata catttcagcg ttgctgtgaa
    12541 ttgccagtcg acgcatcgca ctgttttttt gggcgctgat agttttatta cttttcttaa
    12601 gcaacgaaac tatctggttg atgccccatc ctttacctaa tagccgcagt accttgcgct
    12661 ctgaaagagt gagaatgacg gagcgatagc taaaatcatg agtttcattc attgccggag
    12721 aggtcagcat ggcgctgata cgctctgcgt gcgtattgcc ggaacgtacg gtcttgacca
    12781 gattctcaat gggttcaaca tcagacaata acgtcgccgt agggcacatg agcaattcta
    12841 ctgcctggga ataccaggag cgtgacacca tgaaaaccca gtgaatttcc cgatattgtg
    12901 agattaaaga ataataatgt tcacaaacag aacgtggatc ttcactttca ccggctaaat
    12961 cagcaatgac taatccggaa cgacgtaatt gtaataaggt aaggtcctcc gcagaggcgc
    13021 tggatattat ttcatattca gggaagtgag tcctcatgac ctcttttaac cctgcttgca
    13081 taacaggtat tttactgata acaattccat ttttgcagca tcctggcaac atatttcctc
    13141 cacatccact gtctaaaaat catgatggtt tattcaggat attcattcct taaatgtttc
    13201 cagcaaatta atagattaag cgaataaatt atccttagca ttcactttca tatcagcata
    13261 ctcctgctgc acaacaactc cactgcatgt gtcagaaggt tagcatcaaa tgataacctg
    13321 gtgaaatagc ctggagacat ttaaaatatc tacattaagt tgttaggaat acttaatgaa
    13381 aaacagaaat cacattcatt taatttataa ttaaatgata tcccttcccg cagaatgaaa
    13441 aatcacacaa ccaatattta ttcaatgaaa tctgatggtt ttaacttgtt aacattaaaa
    13501 aacgtgcccg gttatctatt atcctgattc gtaaaggcca aacaataaat tcattatagt
    13561 gagaatattc atgtgatagt ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc
    13621 ttgtcccgat aaccaggatt aatcagcgca attttgatct taacctaccg caaaacaacc
    13681 gtgcttcatg atacaaatgg attctcatta tctgaacaat acgcagcacg tctatgacaa
    13741 agggcgagtt atgcaaactg agcaacagcg agccgtaaca cggctttgta tccagtgtgg
    13801 attatttctt ttgcaacatg gtgcggaaag cgcgttggtt gatgagcttt cctcacgact
    13861 gggtcgggcg ttggggatgg acagcgtcga aagttctatc tcttcgaacg ccatagtgct
    13921 gacaaccatt aaagatgggc aatgcctgac atcgacacgt aaaaatcacg atcgcggcat
    13981 taatatgcat gtggtgactg aagtccagca cattgtgatt ctggcggagc accatctgct
    14041 ggattacaaa ggcgtagaga aacgatttag ccaaattcag ccattacgtt acccaagatg
    14101 gctggtagcc ttaatggttg gcctttcttg cgcctgtttc tgcaaactca ataaaggtgg
    14161 ctgggatggt gccgtcatca ccttctttgc cagtacggcc gcgatgtata tccgccagct
    14221 gctggcacaa cgtcatcttc atccgcagat caacttttgc ctcaccgctt tcgccgccac
    14281 caccatttcc ggattgcttt tgcaacttcc cactttcagc aatacaccca ctatcgcgat
    14341 ggccgccagc gttctgctgc tggtgccggg atttccgttg attaatgccg tcgccgatat
    14401 gtttaaaggc cacatcaata ccggactggc acgctgggcg atcgccagtc tgctgacact
    14461 ggctacctgc gtcggcgtgg tgatggcact gacgatttgg gggctacgcg gatgggtgtg
    14521 atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg ccgccattcc agcggtcggc
    14581 tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct ggtgtgcgct gcttggcgcg
    14641 attggtcatg gttcacgaat gatcttaatg accagcgggc tgaatattga gtggtcaacc
    14701 tttatggcct ccatgctggt cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg
    14761 catccgaaag tgtttaccgt ggcggctgtt atccctatgt tcccgggcat atcggcttat
    14821 accgcaatga tttcggcggt aaaaatcagc cagttaggtt acagcgaacc gttgatgatt
    14881 accctgttaa ccaactttct tacagcttca tcgattgttg gtgcgttatc catcggtctt
    14941 tccattcctg gattatggtt gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa
    15001 agcacacgga tgagagacag cctcctctcc tccgtgtgtt actataaaag ttatctccct
    15061 tctcgttcat cgttccatat ttgagaaaca gtatgtcttc cagagttttg accccggacg
    15121 tcgttggtat tgacgccctg gtacacgatc accaaaccgt tctggcaaaa gctgaaggcg
    15181 gtgtggttgc cgtatttgct aacaatgccc cggcgtttta tgccgtcacg cctgcacgcc
    15241 tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc gggaagcgat gtcgctctgg
    15301 acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc tgtacccatg gggaaattcg
    15361 ccatgtatcc ggactggcaa cccgatgccg attttatccg cctggcggcg ctatggggtg
    15421 tggcactaag agagccggtg accaccgaag aactggcctc attcattgcc tactggcagg
    15481 cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa actggcgcgc agcctgcaaa
    15541 tcggtcgtgc cagcaacggc ggactgccga aacgagatgt gaatacggtc agcgaacctg
    15601 acagtcaaat tccaccagga ttcagagggt aacgatgaaa aacgttggcg acctgatgca
    15661 acgcctgcaa aaaatgatgc ctgcccatat caaacctgca ttcaaaacgg gtgaagaact
    15721 actggcctgg cagaaagaac aaggagcgat ccgctccgcc gctctcgaac gtgaaaatcg
    15781 ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt cggccactgc atcagaactg
    15841 ctcctttgag aactatcgcg ttgagtgtga agggcagatg aatgcgttaa gcaaagcgcg
    15901 ccagtacgtc gaagagtttg acggcaacat cgccagcttc atcttttctg gtaagccagg
    15961 aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag ctgctgctac gcggtaaatc
    16021 cgtattgatc atcaccgtgg ccgatattat gtcggcgatg aaagatacct tcaggaatag
    16081 cggtaccagt gaagaacaac tgcttaacga tctgagcaac gttgatctgc tggtgatcga
    16141 tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg atcatcaacc agatcgtcga
    16201 tcgccgctct tcttccaaac gcccaaccgg gatgctgacc aacagtaata tggaagagat
    16261 gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc ctgggtaaca gtttgtgggt
    16321 gatcttcaac tgggatagct accgtagccg ggtaacaggt aaagagtatt aatccccgtt
    16381 tctggcggta tactgcgaca caattccacc ttgagtaatg atgatgaaaa ctgttaaaca
    16441 tcttctgtgc tgtgccattg ccgccagcac attaattccc accggggtgc atgctgcgtc
    16501 ctggaaagat gcgctctcca gcgccgccag cgaacttggc aaccaaaaca gcacgacaca
    16561 ggaaggcggt tggtcgctcg cgtcattaac taacttgctt agcagcggga accaggcctt
    16621 aagcgcagat aacatgaaca acgccgcagg cattctgcaa tactgcgcga agcaaaagct
    16681 ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg ctggaaaagc tgggcctgaa
    16741 cagtgaagag caaaaagaag acaccaacta tctggatggc attcagggtt tgctgaaaac
    16801 caaagatggt cagcaactca atctggataa catcggaacg actccgctgg cagaaaaggt
    16861 gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg aacttcattt cctgaaccgc
    16921 gaaacgccgc gttttagctg ttacccggct gacgcggctc tctcctgccc ttttccctgc
    16981 ttctggacgg ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat
    17041 aaactaattc ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg
    17101 ccattacatt gttcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg
    17161 atcgcccgtt gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg
    17221 catggaaagc gggacgtaac acctggtggt ttgcagccac attaacggtg ctggggctat
    17281 ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg
    17341 acgcggttct ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc
    17401 tgccgggtat cggcattgtg ctggggctga cagcggtgtt cggtgcgctg ggctggatcc
    17461 tgcgccatcg tcgccatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg
    17521 cgctgggttc ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc
    17581 agtcacgcga tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc
    17641 ctgacccgaa actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg
    17701 ataacgaagc tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg
    17761 atttcagcca cacgcagcag ctaccaggaa cggattacac gattgcgggc atggtggctt
    17821 ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa cgcctccgcc tctgtctcca
    17881 gcttcttccc gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact
    17941 atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga tgtgttcctg aagtcgcatg
    18001 gcttcgacca cttatacggc tcagaagagc tgaaaagcgt ggtggccgac ccgcactatc
    18061 gcaacgactg gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag
    18121 agctttcccg ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc
    18181 cggatggttt tatctcgcgt acctgtaacc gcaaaaaata tgattttgac ggtaagccga
    18241 accagtcatt cagcgcggta agttgcagcc aggagaatat cgcggcgttt atcaacaaaa
    18301 tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt ctcttctgac catttagcga
    18361 tgaacaacac ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca
    18421 ttcgtggcga caagccgcag caagagacgc tggcagtgaa gcgtaacacg atggataacg
    18481 gcgcgacggt gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt
    18541 tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga
    18601 agccggatat catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc
    18661 agcagaaaaa catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg
    18721 tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc
    18781 agctggccga tttcgctcca cgcgacaatt tcgtctgggt tgaccgttgc tataagatgg
    18841 cacaactctg ggctccggaa ctggcactct ccaccgactg gtgtgtctcg caagggcagc
    18901 ttggcggtca gcaaattgtt cagcatgttg acaaaacaac atggcagggc aaaacggcat
    18961 ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa tgtcgatacg ctgaaaattg
    19021 ttgataacga tattcgctac aaagccgaca gtttcatctt caacgtcgcc ggtgcgccag
    19081 aagaggtgaa acagtttagt gggatttccc gtccagagtc gtggggccgc tggtccaacg
    19141 cgcagctggg cgatgaagta aaaatcgagt acaagcatcc gctgccaaag aaatttgacc
    19201 tggtgattac cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag
    19261 gtaatgaaga acaaaccctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg
    19321 ataacccaac cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg
    19381 aagggaatat cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag
    19441 tggtggaacg tgaaggataa ttcctgactg atgtgtaggt cgcataaggt gcgacctaca
    19501 caccttctgc ggcctacaaa acatcattga gaaacagaaa aaaacagcgc ccaacacttc
    19561 cccggacgct gcatggcaac tttaataatc tcgccttatc agaaagtaat tacaccctta
    19621 atcaactcac gattgttaat cacatcgcgc tcgtaggttt ctgccagcgt ggcgaacgga
    19681 tagcgatggg ttaacatcat gtcagcagtg atttttcctt ccgccatcag tcgaccgact
    19741 ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca tcgtcgtttc ttttttatgg
    19801 aattccggat cggagaactg caactcacct ttaaacaggc cgacaaatac caccgtgccg
    19861 ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt gattacctgt cgcgtcgatc
    19921 actttctgcg ccagcgaacc accaaactgc gcccgtagct gcgcgtcaaa atcttcggct
    19981 gacgggtcca gtaaaggtaa ttccagacgc gttgccacat gttcacggcg cgccggactg
    20041 gtatccgcca ccaccacctg tgcgccatcg gctttagcga ttgccgccgc gcccagaccg
    20101 attggccccg ccccgaccac cagcacctgc tcgccgggag caatggctgc gcgacgcacc
    20161 gcatgagcgc taatagcgaa aggttcaatc aatgctgccg cctgcgggtc aataccgtct
    20221 gcgggcaaaa tgttcgccac cggcacgctc aaatactcac taaaaccgcc atcctgatgc
    20281 acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc cgcttttaca cgccgggcat
    20341 tgctgacagg caacataagg gatcacagca acttgctgac catttttaag atcagcaata
    20401 tttttaccca gcctaacaat ctccccacat atttcatggc ctaaaacacg tggataacta
    20461 aaaaatggtt gatttccacc ccaggcatga atatctgtac cgcaaatccc gacagactta
    20521 atttttatta atgcttcatt gtcacccgga ataggtatct cgcgttgttt ccagactaat
    20581 tctttcggct gctggcaaat taaaacattc atcgtagaca tgattcggct ctccattttt
    20641 tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg aacggaaaat ctgttttgtg
    20701 aaaacacacg cataaaaatg ttttaaatcg ggttttaatg gactcacaag gaggaaaata
    20761 tgagtcgttc acaaaattta cgccacaatg tgattaacca ggtcattgat gatatggcgc
    20821 ggggtcatat cccttcccca cttccatcgc aaagtgcgct ggcggaaatg tacaacatca
    20881 gccgcaccac agtgcgtcat attctcagcc atttacgcga atgcggcgtc ttgacgcagg
    20941 ttggaaacga ctatgttatt gcgcgtaaac ctgaccacga tgacggtttc gcctgtacta
    21001 ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc ttttttcacg ctgataaacc
    21061 agcgccagtt acgcccaggg gaaacttttt ctgaactgca actggctcgg gccgcgggtg
    21121 tcagcccggt ggttgtaaga gaatatcttt taaaattcgg ccgttataat cttattcaaa
    21181 gcgaaaagcg cggccagtgg agcatgaagc aattcgatca atcctacgcc gagcagctgt
    21241 ttgagctacg cgaaatgctc gaaacacatt cacttcagca ctttctcaac ctgcctgatc
    21301 atgatccccg ctggctgcag gcgaaaacca tgctcgaacg tcatcggcta ctgcgtgaca
    21361 acatcggcaa tagctttcgc atgttctcgc aacttgacag agattttcac tccttactgc
    21421 tttcagctgc cgacaatatc ttttttgatc aatcgcttga gagcatctca gtcatctttc
    21481 attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt gccgtcgatg
    21541 agcatatgac cattctcagc gctctgattt gccgcagtga cctcgatgcc acccttgcgc
    21601 tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat gagaacacgc
    21661 gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgagtgca cggttttatc
    21721 aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg actaaaaatg
    21781 tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc cgttaatgtc aaccttgcgt
    21841 aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt ggaaaaagaa
    21901 aatatcacca tcgatccgcg ttcttcattt actccatctt cgtcggcaga tattcccgtg
    21961 ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac caccgccatg
    22021 ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct gtcggtagca
    22081 aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc tttgctctcc
    22141 gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg gcccactatt ggatcgtaaa
    22201 ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca ggcaatgtct
    22261 ggcatggtgc acaactttac gcagttcgtg ttggtgcgta tcggtatggg gattggtgaa
    22321 gcgccgatga acccatgcgg tgtaaaagtc attaacgac
//
LOCUS       DAJTMK010000061        21405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTMK010000061 DAJTMK010000000
VERSION     DAJTMK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21405
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.062"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(57..221)
                     /locus_tag="OO135_001935"
                     /pseudo
     CDS             complement(57..221)
                     /locus_tag="OO135_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(300..394)
                     /locus_tag="OO135_001936"
     tRNA            complement(300..394)
                     /locus_tag="OO135_001936"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(358..360),aa:Sec,seq:tca)
     gene            687..2069
                     /locus_tag="OO135_001937"
     CDS             687..2069
                     /locus_tag="OO135_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR5397796.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2079..4370
                     /gene="yicI"
                     /locus_tag="OO135_001938"
     CDS             2079..4370
                     /gene="yicI"
                     /locus_tag="OO135_001938"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5397797.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4423..6132)
                     /locus_tag="OO135_001939"
     CDS             complement(4423..6132)
                     /locus_tag="OO135_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5397798.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSALRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETIPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6253..7644)
                     /gene="xanP"
                     /locus_tag="OO135_001940"
     CDS             complement(6253..7644)
                     /gene="xanP"
                     /locus_tag="OO135_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5397799.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGAALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7924..9129
                     /gene="gltS"
                     /locus_tag="OO135_001941"
     CDS             7924..9129
                     /gene="gltS"
                     /locus_tag="OO135_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5397800.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9163..11244)
                     /gene="recG"
                     /locus_tag="OO135_001942"
     CDS             complement(9163..11244)
                     /gene="recG"
                     /locus_tag="OO135_001942"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5397801.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLDIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11250..11939)
                     /gene="trmH"
                     /locus_tag="OO135_001943"
     CDS             complement(11250..11939)
                     /gene="trmH"
                     /locus_tag="OO135_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5397802.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11946..14054)
                     /gene="spoT"
                     /locus_tag="OO135_001944"
     CDS             complement(11946..14054)
                     /gene="spoT"
                     /locus_tag="OO135_001944"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5397803.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14073..14348)
                     /gene="rpoZ"
                     /locus_tag="OO135_001945"
     CDS             complement(14073..14348)
                     /gene="rpoZ"
                     /locus_tag="OO135_001945"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5397804.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14403..15026)
                     /gene="gmk"
                     /locus_tag="OO135_001946"
     CDS             complement(14403..15026)
                     /gene="gmk"
                     /locus_tag="OO135_001946"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5397805.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIKALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15284..16966
                     /gene="ligB"
                     /locus_tag="OO135_001947"
                     /pseudo
     CDS             15284..16966
                     /gene="ligB"
                     /locus_tag="OO135_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
     gene            complement(16963..17580)
                     /locus_tag="OO135_001948"
     CDS             complement(16963..17580)
                     /locus_tag="OO135_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5397806.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(17872..18696)
                     /gene="dinD"
                     /locus_tag="OO135_001949"
     CDS             complement(17872..18696)
                     /gene="dinD"
                     /locus_tag="OO135_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5397807.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19276..19788)
                     /locus_tag="OO135_001950"
     CDS             complement(19276..19788)
                     /locus_tag="OO135_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5397808.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVDSSW"
     gene            complement(20392..20673)
                     /locus_tag="OO135_001951"
     CDS             complement(20392..20673)
                     /locus_tag="OO135_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5397809.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWMEVMLLCTEDDDREWIKRRRE
                     TCLENVKRPPVKVEEFGDLHKAVTETQHRMGIAQPNGNAFRLNGGKRQR"
     gene            20932..21387
                     /locus_tag="OO135_001952"
     CDS             20932..21387
                     /locus_tag="OO135_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397810.1"
                     /translation="MVFAMSKSNLIAFRIPSELQDEFNRSVLASGGDKTSWLVDAIRM
                     KLGQPEKSIDSRMLGLVERMEKAAASLIAGKPNIPPKPYNETAVIKIIADTIQQGFDN
                     GRVIAERINEAGYQTKAGKAWDKDIYSAWKRQGSNAENLKAVIDCKVSV"
BASE COUNT         4944 a         5380 c         5695 g         5386 t
ORIGIN      
        1 tagtcgtata aaaaagggtg ttatggagcg tgtaaaacaa catcttcacc ttaatggcga
       61 tacagttggc tgagagccag cagggggcaa atcacctgca aataaacacg gcacatgctg
      121 gcgcgcatat cgcggatgcg ttcgaggaca gaagaaccga caggcggatt cttcagccgc
      181 tcatcgtcca tcacaaaacc ttagaccagg tattcctgaa gccgattgaa tattgagcag
      241 tagaaaatat ctggattgac agtattacaa ggggttggag aacgtcagaa gaggcatttt
      301 ggcggaagat cacaggagtc gaacctgtcc gggaccgctg gcggccccaa ctggatttga
      361 agtccagccg cctcaccgga gacgacgatc ttccgcgcct cgattgctac atggaggcgg
      421 ggcgcattat agctacttcc ttgagtttct acatccccca gatcagtttt ttccgcctaa
      481 tacacgcagc gtattatcac gaaagcatta tagttcatat aaaacccata ttttacaatg
      541 atatatgagc ggaattaacg ctaccacgat cacataaaac aagaatcata aattaataac
      601 cagatatcgg aatattcgct ctcacaggga tggtttacaa aatgcgttat cggtctcagc
      661 acccctatag catcaaggaa aagcagatga agagtgaagt gttgtccgtt aaagagaaaa
      721 ttggttatgg catgggagac gccgccagcc acattatttt cgataacgta atgttatata
      781 tgatgttctt ttataccgat atttttggca tacctgccgg ttttgtcgga accatgtttt
      841 tggtcgctcg tgcactggat gcgatttccg atccttgcat ggggttgttg gccgatcgaa
      901 cgcgctctcg ctggggtaaa tttcgtccgt gggtactgtt tggcgcactg ccattcggga
      961 tcgtctgtgt actggcctat agcacgccag atctcagtat gaacggcaaa atgatctatg
     1021 cagcaattac ttacacccta cttaccttac tttataccgt cgtcaatatc ccttactgcg
     1081 cattgggtgg tgtaatcacc aatgacccga ctcagcgtat ctcgctgcaa tcctggcgtt
     1141 ttgtgctggc gaccgcggga ggcatgcttt ctactgttct gatgatgcca ctggttaatt
     1201 taattggcgg tgataataaa ccactcggtt tccagggcgg tatcgcggtc ctttccgtgg
     1261 tggcattcat gatgctggca ttttgtttct tcaccactaa agaacgcgtt gaagcaccac
     1321 ctacaacaac gtctatgcgg gaagatttac gtgatatctg gcaaaacgac cagtggcgga
     1381 ttgtcggttt actaaccatt ttcaatatcc tggcggtgtg cgtactcggt ggggcgatga
     1441 tgtattacgt cacatggatt ttgggcacgc cggaagtgtt tgtcgctttt ctcaccactt
     1501 attgcgtggg taacctgatt ggttccgcac tggcaaaacc tctgaccgac tggaaatgta
     1561 aagtcactat cttctggtgg acgaatgccc tgctggcagt gattagcctc gcgatgttct
     1621 ttgttcccat gcaggccagc atcactatgt ttgtcttcat cttcgtgatt ggtgtgttgc
     1681 atcaactggt gacacctatc cagtgggtaa tgatgtccga taccgtcgac tacggcgagt
     1741 ggtgcaatgg taaacgcctg accgggatca gttttgctgg cacgctgttt gtgctcaaac
     1801 tggggttggc cttcggcggc gctcttatcg gctggatgct ggcttatggc ggatatgatg
     1861 cggcagaaaa agcgcagaac agcgccacga ttagcatcat tattgcgcta ttcacgattg
     1921 ttccggcgat ctgttatttg ctgagcgcga ttatcgctaa acgctactac tcactcacga
     1981 cgcacaatct gaaaaccgtt atggaacagc tggctcaggg taaacgccgt tgccagcaac
     2041 aattcacctc tcaagaagtg cagaactaag gaacggcaat gaaaattagc gatggaaact
     2101 ggttgattca acctggcctc aatttgattc acccgcttca ggtgttcgag gttgaacagc
     2161 aggataatga aatggtggtc tatgctgccc cccgtgatgt gcgtgaacgt acctggcagc
     2221 ttgatacgcc tttatttacg ctgcgctttt tctccccaca ggaaggtatt gtcggtgtgc
     2281 ggattgagca ttttcaggga gcgctgaata acggtcctca ttatccgctc aatattttgc
     2341 aggacgtgaa ggtcacaatc gaaaacacag aacgttatgc tgagtttaaa agtggcaact
     2401 taagcgcgcg tgtcagcaaa ggtgagttct ggtcactcga ttttctgcgc aacggtgaac
     2461 gtattaccgg tcaggacacg aataatcaac gaaattatat gtttgagcgg cttgatcttg
     2521 gcgttggcga aacagtttat ggtctgggag agcgttttac tgccctggtg cgcaatggtc
     2581 agacggtaga gacctggaac cgggacggcg gcacaagtac tgaacaggcg tataaaaata
     2641 tcccgttcta tatgactaac cgtggctatg gggtgctggt caatcacccc cagagtgtct
     2701 cttttgaagt gggatcggag aaagtctcca aagtgcagtt cagcgttgag agcgaatatc
     2761 tcgaatactt tgttatcgac ggtccgacgc cgaaagcggt acttgatcgt tatacccgtt
     2821 ttactggtcg tccggcgctg ccgcccgcat ggtccttcgg cctgtggcta accacttcat
     2881 ttaccaccaa ctacgacgaa gcgacggtaa acagctttat cgatggtatg gcggaacgca
     2941 atctgccgct gcatgttttc cactttgact gtttctggat gaaagccttc cagtggtgcg
     3001 attttgagtg ggacccgctg actttcccgg acccggaagg gatgatccgc cgcctgaaag
     3061 cgaaagggct gaaaatctgc gtctggatta acccctatat cggtcaaaaa tcccccgtct
     3121 ttaaagagtt acaagagaaa ggctatttac tcaaacgccc ggacggttcg ctatggcagt
     3181 gggataaatg gcagccaggt ctggcgattt atgactttac caatccggat gcctgcaaat
     3241 ggtacgccga caaactgaaa ggtctggtcg cgatgggcgt tgattgcttt aagaccgact
     3301 ttggcgaacg tatcccaact gatgttcagt ggtttgacgg ttccgatccg cagaaaatgc
     3361 ataaccatta tgcgcacatc tacaacgaac tggtgtggaa cgtgctcaag gacaccgttg
     3421 gtgaggaaga agctgtcttg tttgcccgct cggcctccgt cggtgcgcag aaattcccgg
     3481 tacactgggg tggcgattgt tacgctaact acgaatcaat ggcggaaagc ctgcgcggtg
     3541 gtttgtctat tggcctttca ggttttggct tctggagcca ctatatcggc ggctttgaaa
     3601 ataccgctcc ggcgcacgtt tacaaatgct ggtgcgcgtt tggtttgctc tccagccata
     3661 gccgtttaca cggtagcaaa tcttatcgtg tgccgtgggc ctacgatgat gagtcctgtg
     3721 atgtggtgcg cttcttcacg caactgaaat gccgcatgat gccgtatctg tatcgtgaag
     3781 ctgcgcgtgc aaacgcgcgg ggtacgccga tgatgcgggc catgatgatg gagttcccgg
     3841 acgatccggc ttgtgattac cttgaccgtc aatacatgtt aggcgacaac gtgatggttg
     3901 cgccggtgtt cactgaagcg ggcgatgtgc agttctacct gccggaaggt cgctggacac
     3961 acctgtggca caacgatgag ctcgacggtc gttgctggca taaacagcag cacagcttcc
     4021 tgagtctgcc catttatgtg cgtgataaca ccctactggc gctgggcaac aacgatcaac
     4081 gtcccgatta cgcgtggcac gaaggcacgg cattccacct ctttaatctg caagacgggc
     4141 atgaagccgt ctgtgaagtg cccgatgctg acggatcggt gatctttact ttaaaagcag
     4201 cacgtactgg caacacgatt actgtgactg gtgcgggcga ggcgaagaac tggacactgt
     4261 gcctgcgcaa tgttgtgaaa gtaaatggtc tgcaagacgg ttcgcaggct gaaagtgagc
     4321 aggggctggt ggtgaagcct caagggaatg cgctgacaat tacgttgtaa tctattgatg
     4381 gaatgccggg tacgtctgag tcgtacccgg cgagattgat gtttactgca ccagctctgc
     4441 cgacgtaact tcaccgctgg aaacgatgcc agcattcatg gtttgagtca cttgctgctt
     4501 tgcggcgttc acggcatgca attgcccgct aaccgtaggt ttcagcgggg cattggcctg
     4561 aatatctcca ctggcggtaa gctgaatatt gccgtcgcca gtcaacggta attcaggcca
     4621 tccccattgt tgcaaaatat tcaccggcac accgcgtcca ttcaggctga tatgggtctg
     4681 acgttgtggc gtttgtgaaa cactggcagt ggcttccaga atgccttttt cagtaaatgc
     4741 actcagttcg ctgatattca ccgtgctgct gttggcggtc agcgccagcg acgggcgacg
     4801 aacatcaaca cgattgaatg tcgcggcggc ggcattcaga ttcgccgagc cactccagac
     4861 gccccattta tgatcggtaa ccagcgtcag gttggcaccg taaccatcga gcgtggtgag
     4921 ctgccacggg aagtcagggt cgatatcaat gatcagattg cggctggcgc taaatctctt
     4981 cagttgcagg ctgtttaacc aaccgggtat cgtttccatc cacaactgtt gccagttttt
     5041 cggcaaggta tattccagcc cggcaattgc cgcatcatca aggatcaacg ttttcccgtc
     5101 acgcagccag ttccctgacg ttctgaccat ccccccttcc cagcggctgg tgaactggcg
     5161 cagcgctacg ccctgcgggg aaaattccgc gtttataatc gggtcaaata aatgcagcga
     5221 accataaatg aactcgctag cgttcatcga cagtttgcct tcttgtgtct gccagtcatc
     5281 tttactgaag gtcatgttgc gcaagctgag atcgaggtcg gtcaccgccc agtccggacc
     5341 ttgcaaacga gcatcgatca cttccaggcg accaatctgc aacgacggga cagagcgtaa
     5401 tggcgcaaag aagtcggtta gcgatttttc gctttgtaga cgtatatccg ccatgcgcag
     5461 gttttccact tgccagctgc cgtcggcgtt acgctgcgcg tttccggtta atgtcccgcg
     5521 ggcgatgtcg gcacccaggt tagtcagcgt aacgcgatcg ttatcaatac tgccttcaat
     5581 cagtacattg gtggcaggaa catcattgag cgaaagcgat ccggcactaa actgaatctg
     5641 cgccttcgta cccagcactt taccggcttt tggtgaccac ggaactacgc cgccatttac
     5701 ccgcagcgcg ctcagtttcc attcgctatt cgggctatta aacgccatat cacgcagttg
     5761 cagacgatcg gctttgaacg gtagcggcgc ggtctggtcg gtgagattca gcgtcccgtt
     5821 ttccagcagg atggtatcga catggcgtgg ttcggtcagt tgccgactgc ttagcgcaat
     5881 gtcgacactt ttggccacca gggtcgcggg ctgaccatca cgaccaaacg tgacattctc
     5941 cagcacgata tgagatggcg cagaaaaacg gtgatccatc gccccgaagg ccagatgata
     6001 gtcgctattc tcggaaaccc atgcgctgat atgttctgct ccccagcggg tttgcagaag
     6061 aaaatagagg ccagcgatca ccactaacag agcgatgaga atgtagagaa gcagcttccc
     6121 aataaatttc atggtcttcc atcccgtgaa atgcacataa gagagttatg cacgatttac
     6181 gcgcaatcct caaggcggga atggtgaaag aggtgagact gcggcggtaa ttatcttacc
     6241 gccgccagtg aattactgtt tttctggtgg gaaaatcaga ttcagcacga tggcagtaat
     6301 accgcccgcg gcgatcccgg aggagagcag gtttttcagc cattcagggg caaactgcaa
     6361 aatcagcggc tgctgagaca cgcccagacc aaccgccagc gacagcgcga taatcagaat
     6421 cgcccgacgg ttcagcggct cacgagaaac gatacgcaca ccggaggcgg cgatggtgcc
     6481 aaacattaca agcgttgcgc cgcccagaac cggttctgga atgtgttgta caaaaccgct
     6541 cactgccggg aacagaccca gcacgatcaa catcagcgcg acgacaaaac cgacatagcg
     6601 gctggcaaca ccagtcaact ggatcactcc gttgttttgc ccgaagcagg agttcgggaa
     6661 ggtgttaaac accgccgaaa caaacgagtt caggccgttt gccagcacgc cgcctttcag
     6721 gcgtttcatg tacagcggac cggacactgg ctgttcggaa acgtcagagg tcgacgtgat
     6781 atcgccaatg gtttccagcg aagtgatcat aaagaccagc atcagcggca gcagcagact
     6841 ccattcaatg ccaagaccgt aatagagcgg cgttggcacc ataatcagtt cttgcgtcat
     6901 cggttcgttg ctttctggca acatgcccat aaaccacgcc agcgcatatc cggccgccat
     6961 cgcaatcacc agtgaggcca cgcgtaagta agggttacgt tgacggttaa gcaggataat
     7021 taaggctaag accacgcctg ccagcagcag atttttcggt gcgccgaagg tgttatcgct
     7081 catggctgcg taaccgccgc caatggacgt taacccaacc tgaattagcg acaggccgat
     7141 aatcatcacc acaacgccag aaaccagcgg cgtaataatg cggcgcgcca gatgcagaac
     7201 gcgggagatc accatctcgg tgcaacttgc cagcatcaac gtgccgaaca aagccgccat
     7261 catggtagga acatcagcac caccggtttt cagcgcggca ccgcccataa tcagcggggc
     7321 aacaaagttg aagctggtgc cctgaataga caacagcccg gagccaaccg gaccccaggc
     7381 cttaatttga ataatcgatg ccacaccgga ggcaaacagc gacatactaa taatgtgttg
     7441 cgtgtcttgt gccggtaaac ccagcgcctg gcagattaat agcgctggcg tgatcaccgc
     7501 aacgaacatc gccagcagat gctgacaggc ggcaaacagg gtttgaggaa gcggcggacg
     7561 atcttcaaga cggtaaatca gttcgctgtt ttgagtctgc gcaaccggtt gcgcattttc
     7621 tgactcgagg gtggaaacag acatcgaaaa caatcccgtg gtagcaaagc gggcatttta
     7681 gctgaccgaa agagaaaagc aaacgtttgc cacatcctca tctaaatatt taaatgagat
     7741 aaatgcactt ttttatataa cttttggtat ttttctgctt aaaatccatg ccatttgttc
     7801 attaacagct gagaatttga cctaggaaag cataactcga tacccgtcat agttcatgaa
     7861 gttgccactg cgcgatgaag tatgacgagt atgaaagagt gatgcggata caaaggagta
     7921 actatgtttc atctcgatac tttagcaacg cttgttgccg caacgctgac gttgctgctc
     7981 gggcgtaagt tggtccattc cgtctccttt ttgaagaaat acaccatacc ggaacctgtt
     8041 gcgggtggtt tgttggtggc gctggcgcta ctagtactga aaaaaagcat gggctgggaa
     8101 gtcaactttg atatgtccct gcgcgatccg ttaatgctgg ctttcttcgc caccattggc
     8161 ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg tggttggcat cttcttgatt
     8221 gtggttgttg gtctgttggt gatgcaaaat gccattggca ttggtatggc tagcctgtta
     8281 gggcttgatc cgctgatggg gctgttggcc ggttctatta cgctttccgg cggtcacggt
     8341 acgggcgctg cgtggagtaa attgttcatt gaacgttatg gcttcaccaa tgcgacggaa
     8401 gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg gcttgattgg cggtccggtg
     8461 gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta ttccggatga ccaggaagtc
     8521 ccgacggcgt ttgaaaagcc ggatgtggga cgcatgatca cctcgttggt gctgattgaa
     8581 actatcgcgc tgattgctat ctgcctgacg gtggggaaaa ttgttgcgca acttttggct
     8641 ggcactgctt ttgaactgcc gaccttcgtc tgtgtactgt ttgttggcgt gattctgagc
     8701 aacggtctgt caataatggg cttttaccgc gtctttgagc gtgcggtatc cgtgctgggt
     8761 aacgtaagct tgtcgttgtt cctggcgatg gcgttgatgg ggctgaaact gtgggagctg
     8821 gcttcgctgg cgctgccgat gctggcgatt ctggtggtac agaccatctt catggcgttg
     8881 tatgccatct tcgttacctg gcgcatgatg ggcaaaaact acgatgcggc agtgctggct
     8941 gcgggtcact gtggttttgg cctcggtgca acgccaacgg caatcgccaa catgcaggcg
     9001 atcactgaac gctttggccc gtcgcacatg gcgtttttgg tggtgccgat ggtcggtgcg
     9061 ttctttatcg atatcgtcaa tgcgctggta attaagttgt atttgatgtt gccgattttt
     9121 gccggttaac cgatgaagcg gcggtcttct caccgccgcc ttttacgcat tcgagtaacg
     9181 ttctgtctcc ggcatccagc gttctatcaa ggcttttgcc tgttgtgggt aacgttcgtg
     9241 aatatgtcgc gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc
     9301 cactttaaat tcagcattac ccgtctgacg cgtgcctaac aattcgccag ggccgcgaat
     9361 atccagatct ttttgcgcaa tcacaaaacc gtcgttactg tcgcgcagca cttgcaggcg
     9421 aatttgcgcc gttttagaaa gcggcgtttt gtagagcagc acgcagtgag aagccaccgc
     9481 gccacgacct acgcgcccgc gcagctggtg taactgcgcc agacccagac gctcagggtt
     9541 ttcgataatc atcaggctgg cgttaggcac atcaacaccg acttcaataa cggtagtggc
     9601 aaccagcaga tgtagctcac cttgtttaaa cgacgccatc accgcctgtt tctcggctgg
     9661 tttcatccgc ccgtgtacca ggccaacatt caattctggt agcgccagtt tcaactcttc
     9721 ccaggtagct tccgccgcct gcgcttccag taactctgac tcttcaatca acgtacaaac
     9781 ccagtacgcc tgacgacctt cagttatgca ggcatggcgc acgcggtcaa tgatgtcggt
     9841 acggcgggta tcaggaatag cgaccgtagt cactggcgtg cggcctggcg gcagctcatc
     9901 tatcaccgag gtatcgagat cggcatacgc cgtcattgcc agcgtgcggg ggatcggcgt
     9961 ggcggtcatg atcaactgat gcggatggaa gccttgctgc tggcctttct cccataacgc
    10021 cagacgctga tgcacgccaa agcgatgttg ttcgtcgata atcaccagcg ccagaccgtt
    10081 aaactgcacc tgttcctgga agatggcgtg cgtaccaaca atcatctgca cctgaccgct
    10141 ggcgatggct tcctgctgag acagtcgcgc tttgcctttc tgcttaccgg cgagccagcc
    10201 cacttcgata ccgagcggtt caaaccagtt gcggaagtta ttggcgtgct gttcggcgag
    10261 taattcggtt ggtgccatca atgccacctg tttgccgtgg gcaatcgcac gcagcgcggc
    10321 gagggcggcg accagcgttt taccggaacc tacatcgccc tgtaccaggc gcatcatcgg
    10381 cacatccagc gccatatcgc gctcgatctc cgccactacg cgtgcctgtg cgcccgttgg
    10441 cttgaacggt aaggctgcga ggagtttatt tttcagcgca tcattggcgc tcagcggctg
    10501 ggcatgaaaa cgctgtgctc cggcacgtaa ggctaacatg ctgaggttgt gcgccagcag
    10561 ttcttccaga atcagacgac gttgcgccgg atgctgcccg gtttccagat cgctaagctg
    10621 tagcgtcggt ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg
    10681 tgacagttcc ggcggcagga gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg
    10741 gtcggttaat ttacgcagcg tggcctgctt tacgccttcc gttgttggat aaaccggtgt
    10801 gagcgtttcc tgtaattctg gcgtgctgag atcgccctgc acgcggtatt ccgggtggat
    10861 catctccgca ccatatttac cgcgctttgc ttcgccataa gccagtacac ggcggcccgt
    10921 cgccaggcta tttttcattg ccgcgctgaa attgaaaaag cgcatggtga ggatgccgga
    10981 accgtcgctg atctggcagg tcatcatccg ccgaccgccg aaggagatat tgcagttcag
    11041 cacttcgcct tccaccgtgg cataaacgcc cggcagtagt tctccgatgg ggtagagatg
    11101 ggtgcgatct tcgtagcgca ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt
    11161 gattttcgcc agtttgttac taagtgctgc gccaacgccc gttagggaac tgagtgggac
    11221 agcatctaac aggcgacctg tcatggcact taccctgcag cctgcatagt agcccaccag
    11281 tcggcatcag cttcgatctc gccttgctga ttgacgtggg gataaggcag gcctttgcgt
    11341 tttgcgactt tcgccagcac cggatagccg ccttcaaaca acaggcgttg ttgctctgct
    11401 tccggcaaca tgctgttttc acgcaggtac atgcctgcat tttgccgctg acgctgggct
    11461 tcgtaaagaa tgagggctga ggcaacggaa acattcagcg actgcaccat gccgatcatc
    11521 ggaatgatga tgtcctgatc cgccagggcc aatgcttcct gcgtgatgcc cgttttctcc
    11581 tgtcccatca aaatgcaggt cgggcgagtg taatcaattt cgcggaaatc gacagcgtta
    11641 tcagaaagat gggttgccag aatctgcatg ccctggcctt tgagatgagc gacggcatcg
    11701 ccaatggtgc ggtgtgtttt cacctgtacc cagctgttac tacccgccgc tgccgaagcc
    11761 atggtgcgca tgcggctacc aggccagacg gcgtgaactt catgtacgcc aacggcatct
    11821 gcggtacgaa taatcgcaga aacgttatga ggtttgtgga cctgctccat gcagacggtc
    11881 agatcaggct gccgcctggc gagcatttcg cagatgcgtg cataacgtgt tgggttcata
    11941 aaacattaat ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg gattttgcgc
    12001 atgatattcg ccagatgcac acggtcacga gcggtcagac gaataaaggc gctgtagacg
    12061 cgaccatctt tctcttccgt attcaaactt tgaatattcg aagtcgtggt gttaattgcc
    12121 gccgtcaggt ttgccagcgc accctgatga ttgaacatct ccaccttgat ttcggtgatg
    12181 aactcctgcg ccgtctcttt atcccattcc acagccataa acttctctgg ctctttctgg
    12241 tagccacgga tgttacggca ggattcatgg tggatcacca gacctttacc ggggctgacg
    12301 tgggcgataa tcgggtcgcc aggaataggg cggcagcatt tcgcaaaggt gatcagcacg
    12361 ccatcagcgc ctttgatcgg cagatgcccg tggctttgag tcgccggtgg aatggaggcg
    12421 tccccatgtt gtagattttt cgcgaccacc acgctcattg cgttaccaag gccgatttct
    12481 gccagcagat cgtcaagcgt tgccagcttc atgcgatcca gctcgcgctg aatattttcc
    12541 tgcgggattt cattgagctt acggctacca cccaaagcat ggttgagcag acgacggccc
    12601 aggcttacag aatcatcacg cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct
    12661 ttcgagctaa cgacaaagtt cagccaagcg gcattcgggc gagcgcccgg agcggtaatg
    12721 atttcaacgg tctgaccgct agtaagcggc tgcgacagcg ggtaaggctg gcggtcgacg
    12781 cgtgcgccca cgcaggcatg gccgatatcg gtatgcactg cataagcgaa gtcgacgggc
    12841 gttgcaccgg caggcagctc gacaatgcgc ccttccggtg tgaaaacgta aatctcatcc
    12901 gggaagagat cggatttaac gctctcgata aattcaaacg aactaccggc gctctgttgc
    12961 agctccagca ggctttgcat ccagcgctgg gcgcgtattt gtgcggtagt actggtttcg
    13021 ccgtgctctt tataagccca gtgcgcggca acacccatct ccgccatctg atccatatct
    13081 tcggtacgga tctggacctc aaccggcacg ccgtgcgggc caatcatcga ggtgtgcaac
    13141 gactgatagc cgttcgcttt tggaatggcg atatagtctt tcacgcggcc cggacgcggc
    13201 ttgtacaggc tgtgcatctg gcccagcacg cgataacagg tgtcagaatc attgacgatc
    13261 acgcggaaag cgtagatgtc catgattgag tgaaaacgct gctctttgag caccattttg
    13321 cagtaaatcg aataaagatg cttttcgcga ccactgacgc ggcacggtat tcccgcttcc
    13381 tgcaaacgcc cttcgatttc agaaagaatc ttctggatca tctctttacg gttgccgcgc
    13441 gcggctttca ccacttcttt gattacgcga taacggttgg gatacagcgc ctcaaaaccc
    13501 agctcttcga gttcggtttt aatgtggtgg atacctaaac ggtgcgccag cgggctgtaa
    13561 atttcgagag tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg
    13621 cgcatgttgt gggtacggtc ggcaagtttg atgaggatga cgcggatatc ctgcaccatc
    13681 gccataatca tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg gaacttgagt
    13741 ttatcaagtt tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc
    13801 atatcctggt aggtggcggg agtatcttca atcacgtcat gcagcagcgc cgccatcagc
    13861 gtttcatagt cgagtttcat ctcggccaga atgcaggcaa ccgctaccgg gtgcgtgata
    13921 tagggttcac cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc
    13981 tgccgcagac gcttgatttg gtcttccggc aggtaggttt gaatcagttg attcaggctt
    14041 tcaaacagat acaagggcga cccgctttgt gattaacgac gaccttcagc aatagcggta
    14101 acggcttgta attcagcggc ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg
    14161 atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg
    14221 ttttcttccg gtaccagcgg atcctttccg cctacctgca tctgacgagc gcgacgcgcg
    14281 gcgaccagta ccaggtcaaa acggttacca attttctcta cagcgtcctg aacagttacg
    14341 cgtgccatac ttaaaaagct ccacaggtga agaaatgact gggcatgata ctgaaatcag
    14401 gttcagtctg ccaacaattt gctgattaaa gcgtcatgac gctgcttttg gcggctcatg
    14461 cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca acgcggtatc gaagtcatca
    14521 ttcacaatca ggtaatcata ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata
    14581 cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg gtccagttca
    14641 attttagacg gcggtaaaat aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc
    14701 tgcgcgccct gccagtcgat atcgagaaaa acatcgacac cggtcgccag tacttgctca
    14761 atggcctcac gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca
    14821 tctctgctaa tcatttcttt aaattcatca tgattaacaa agaaataatg ttcaccgtgg
    14881 acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac
    14941 ggttgggttt ttaataaagc cttaatcagg ctggatttac ccgcgccact gggggcagaa
    15001 acaatataaa gcgtgccttg agccatgagt atctttcgta tgtgattagc gaaataaagc
    15061 ctacatacga gcttattata cacggcgcgg ccatgtgacg tagcccttgc tcacactttt
    15121 tgcgcaaagt tgttgcgttt tgcgtagctg cttccgcatt tgcctgtgat ttactgcaat
    15181 aaagacgatt ctcaggaagc gtcctcgcag actccgtttt tgccactcgg ctttgccttc
    15241 accacgttgt atatctgcca gcctgactac agcggaggtg aagatgaaag tatggatggc
    15301 gatattaata agtatcttgt gctggcaatc atctgtgtgg gcggtctgtc cggcctggtc
    15361 gccagccaga gcacaggaag aaattttccg ccagcaacag caaataaaac agtgggacga
    15421 tgactactgg aaggaaggaa aaagtgaggt ggaagacggt gtttacgatc agttaagcgc
    15481 ccgtcttacg cagtggcaac gctgctttgt gagcgagccc cgcgacgtga tgatgccgcc
    15541 tttaaatggt gcggttatgc atccggttgc ccatacgggt gtacgcaaaa tggcggataa
    15601 aaacgcatta agtctgtgga tgcgagaacg tagcgatctt tgggtgcagc caaaagttga
    15661 tggcgttgcg gtaaccctgg tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg
    15721 taatggcctg aaaggcgagg actggacgca gaaagttagc ttaatttccg ctgtgccgca
    15781 aaccgttagc gggcctttag ccaacagtac gcttcagggg gaaatatttc tccagcgcga
    15841 ggggcatatc caacaacaaa tggggggaat aaatgcccgc gcaaaagttg ctggcttgat
    15901 gatgcgccag gacgatagcg acacgctgaa ttctctgggc gtttttgtct gggcatggcc
    15961 ggatggaccg cagttaatgt ctgatcgttt aaaagagctg gctaccgcag gttttactct
    16021 aacgcagacg tatacccgtg cggtgaaaaa tgctgatgaa gttgcgcgcg tacgcaatga
    16081 gtggtggaaa gcggaattac ccttcgtcac cgatggcgta gttgtacgag cggcgaaaga
    16141 gccagaatcc cgccattggc taccgggcca ggcagagtgg ctggtggcct ggaaatatca
    16201 acctgtagct caggttgccg aagtgaaggc gattcagttt gcggtgggta agagcggtaa
    16261 aatatcggtg gttgcgtcac tcgcatctgt catgctggat gataaaaaag tccagcgggt
    16321 gaatattggt tccgtcaggc gctggcagga gtgggatatt gcgcctggtg atcagattct
    16381 cgtcagcctt gccggtcagg gtattcctcg cattgatgat gtggtgtggc gcggtgcaga
    16441 acgtacaaaa ccgacaccgc cagaaaaccg ctttaactcg ttgacctgct actttgcttc
    16501 tgatgtttgt caggaacagt tcatttcacg cttagtctgg ctggggtcaa aacaggttct
    16561 tgggctggat ggcattggtg aggccggttg gcgcgcgctg catcagactc atcgctttga
    16621 gcatatcttt tcttggcttt tattaacgcc agagcaatta cagaacacgc cggggatcgc
    16681 gaaaagtaaa agtgcgcagt tatggcatca gtttaatctg gctcgtaagc agccttttac
    16741 tcgctgggtg atggcaatgg gaataccgct aacccgggcg gcgcttaatg ccagtgatga
    16801 acggtcctag tcgcaacttt tatttagcac ggagcagttc tggcagcagc tgccggggac
    16861 tggatctggg cgcgccagac aggttattga atggaaggaa aatgcgcaaa tcaagaagct
    16921 gggcagttgg ctggctgccc agcagatcac aggttttgaa ccttagtgac cttcgtggct
    16981 gtagtaaaag accggtaatc ccagcttaag acgtagcgcc agtaagcggg caaaaaagcc
    17041 gaataccagg gtggagataa tcaccacatc atggttagaa acatagtgtt gcagcgcgat
    17101 gtacagcacg gcggaggcaa atgagacacc agcatacaac tctttctgga ataccagtgg
    17161 gatgcgttta cagaacatat cgcgcaaaac gccgccaaac acgccggtgg ttaccgccgc
    17221 gacaacggca ataattgggc cgtgccccat atccagtgca acctgtgcgc cgatgataga
    17281 aaagactacc agtccgagcg catcgagcac cagaaacact ttgcgcaggt aaggcattac
    17341 aggagcaacg atagtggtga gtactgcggc ggtagcgacg ataatcacat attccgggtg
    17401 tttgacccag ccgagcggat agtggcccag cagaatatcg cgcactgacc ctccgccaat
    17461 tgcggtggcg gtcgcaataa taattacgcc aaatgtatcc atgcgccgtc gtccggccgc
    17521 cagcgccccc gtcatggctt cggcagtgat gccaaccaaa tacaaaatgt gtaacagcat
    17581 ggttctctcc ctaaaaatct gtctggcagg gtagcgattt gtgcgcattg tcacgattga
    17641 gattttttaa ggtaagagga aattgataac ttgtagttat gtgaaatcgc tattttctgt
    17701 agcgtagatg cattattctg agttcttgtc tgagaaaatg cataagaaaa aataggttaa
    17761 aattgagggg tggaaaagaa aggtgcaatg tttagccatt gtgttcactg cacctggact
    17821 ttggcttacg catcgggtat ctcaattaag aaaaagagtt tttagtttct gctatttctt
    17881 ctctgtaatt tttacactgt tttccaactg cttgatgctg acctgcgggg tcggcaactc
    17941 ctcaggcatg gttccgccaa gttcctgaat ggtttgcctc accttgcgac ccacgtcaaa
    18001 gtgtgtggtg tttgcttgct gttttgaatt cacgccatcc cgcttgagtt tttcttctgt
    18061 ttgggtagct cgaaagagat tagccgccag ttctgttgaa cccatatgat ccaggatatt
    18121 ctgattcttt ttcagcccct tccgctgatg aatagctttc tgatctaatc caccatacag
    18181 cccccggtaa ccatgattct gaaatatggc gaaatcgata gctgtcgcta ccccagcttg
    18241 ctgcgctgcc tcaaccaatt gtttgttatg ttctttcaat tcatttctca gaaaaagacg
    18301 cttttcgtct tcacgaagtt gcctgaaagc ttcatcgtca gccagctcct gccgtcgggt
    18361 ctggatagca aaataagttt gccctgccgc aataaccggt ttcgcagggt cgccgttttg
    18421 tactaccagg taacaggcat agcgggagag atgaacatct tcaagttctc gttgagcccc
    18481 tgaacctaat acaaccattt tggtggtttc aacgaaatgg tcagatgcag cttgattgct
    18541 ggcttcgcac gcttgagtcg cgcgggccag taccttttga aaattgcgcc agtcccgata
    18601 atccagtaac ggtgccaatt ctctagctga ccaaaattct tgcccttcag tgccataatg
    18661 ccgtatttct tcaaaaggtt gatgatgttc gttcattgcc cactccatgt gatatcggtt
    18721 gcaaagaaag taaatctgta actgtattta tatacagttg tgagtatctc acgttctcag
    18781 tgtgctgggc aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag aattgaagtt
    18841 gcggggaaga aaaatgccgc tctgtttgag tgggcggcat ttgtgaaggc tgaaaagtaa
    18901 gaattggcgt tacgagttga atctgtttat tgagtagtgg ctttaaacgc ccgtggttgc
    18961 tgtgtttctt gtaatgcgtt ttctcagtag gtataccctt gtgtacacgg tattttgagg
    19021 cgctgtcttt ggctgctcgt tttatgctct catgcttgct gttctggcct cttatctttt
    19081 gcgccttatt gtatgatagt gctatcccac aataaggaac ctgccagcta tgagaatgac
    19141 cagccgcaaa aaagagatac ttagctactt tgaaccggat aacctcgaat gggtgactgg
    19201 tgagattggt gcgccgccgt tcgatgtgtc gggtgtggcc tacctgcttc atggcatggt
    19261 gtcgttcgat aagcgtcacc agctcgaatc aacccggcgc tcaatatcca gcatcaggaa
    19321 attgtggatc tcggcctgct caggattatc agactctctg gcctgtaata gcaattcatt
    19381 gtaaccatgc aagccgcctg agttttccac cggggtggtg cctttcccac gcaggcagct
    19441 aatgggctga ttgctcgtat cgtgtgtttg cttctccagc acaatgacgc aatcccacca
    19501 gtcgccaaag tcatacgtgt aaaccagctt gtcaccatca ttctggagcg ctgcccatag
    19561 tggtgtgatt gccgcattac ccatgctttc atcctcaccc catacgcgat actcgctacc
    19621 gggatcacct ttacgccctt tctcgaacat atacaggtgt tcgtcattcc agccaaaagc
    19681 caactggatc accgcgtgaa gttcactaaa ggggatttgc tctggaacca gcacacgccg
    19741 ccatataggt ggtttgctgt ctcggatggt tatctttagc tggtacatgc aaatcctgaa
    19801 taaatgcgaa tcggaaggat aacgatactg acgtactcac aattctgtaa aggtgaataa
    19861 aggaaatttg cttaatacgc ataaggtaca tgtggaataa tcccatatct acctgcgcgg
    19921 cgctggcttc ttccactggt aggcaggtgc tcgcattatt tcgcggaacc tgtcgcgccg
    19981 ggctatatcg gcattaactg atcgttctat ctcccgttga tctgcctgtg taagctggtg
    20041 gcctccgtgc tcaagtttgg cgatggtcaa taaggctatt tcgcgtggtg tcatacgtca
    20101 taccctctgg aaggatgaaa cacgcccgga ttaaatttca tttttcgcgg gtataaaaaa
    20161 acgatggggc gggcagtccc ggatagtcag ggctgtagag atttgactac cccctcccca
    20221 ctggattcag gttactgcta ttccgttaag cgctgcttac ggggatagct gcgttgtgat
    20281 tttgaccatt caggtcgccg ctatttcctc accgattggg tgctacgcgc gcgcgtactt
    20341 gtcccgaaat accagaaacc gccagccaga ggatgatttc cctctggtgg tctacctttg
    20401 cctcttgccg ccatttaacc ggaaggcgtt accgttcggt tgcgctatcc ccatgcggtg
    20461 ttgcgtttcg gtaacagctt tatgcaggtc gccaaattcc tcaaccttca cgggcgggcg
    20521 cttcacgttc tccagacacg tttcacggcg gcgctttatc cattcccgat catcgtcctc
    20581 ggtgcataac agcatgactt ccatccatcg tgcggcagct ctccggtata gcccttttga
    20641 ctccagctct tcagctttcc tgtcccttat catttctgcc tctcagatta tatagataag
    20701 caaaaaggtg ttggttcagt tggttcagtt ggttcaattt gtaaagatcg ttgtttttat
    20761 tatgtttttc taaaatattg aaccaacatc aactcatttt gaaccaacac agcccagctc
    20821 tgaaccaaca acacagcgat agctttgtga ttggtccgtt attttctggc tgatatttga
    20881 gcgtataaca gtgttataca gtggtatact gtatggatat tactcactga tatggtcttt
    20941 gcaatgagca aatctaatct gatcgcgttc cgcatcccat ctgaattgca ggatgagttt
    21001 aatcggtctg ttctcgcttc tggaggggat aaaacctcct ggcttgttga tgcgattcgc
    21061 atgaagctgg gacagccgga aaagtctatt gattcacgaa tgcttgggct ggtggagcgc
    21121 atggaaaaag ccgctgcgtc gcttattgcc ggaaagccga acatcccacc aaagccatat
    21181 aacgaaacgg cagttataaa gatcattgcc gataccatcc aacagggatt tgataatggg
    21241 cgtgtgatag ctgaacggat caatgaagca ggctatcaaa caaaagctgg taaagcgtgg
    21301 gataaagata tttacagcgc gtggaagcgt cagggaagca atgctgaaaa tttaaaagcg
    21361 gtgattgatt gtaaggtcag cgtataaaca tgagtgccca ttgga
//
LOCUS       DAJTMK010000062        21310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTMK010000062 DAJTMK010000000
VERSION     DAJTMK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.063"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..777
                     /gene="umuC"
                     /locus_tag="OO135_001953"
     CDS             <1..777
                     /gene="umuC"
                     /locus_tag="OO135_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5397811.1"
                     /translation="DLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLAD
                     TDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQA
                     ICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIIN
                     AATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNA
                     KEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(823..1353)
                     /gene="dsbB"
                     /locus_tag="OO135_001954"
     CDS             complement(823..1353)
                     /gene="dsbB"
                     /locus_tag="OO135_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5397812.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1499..3040)
                     /gene="nhaB"
                     /locus_tag="OO135_001955"
     CDS             complement(1499..3040)
                     /gene="nhaB"
                     /locus_tag="OO135_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5397813.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLIEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMR
                     WIATL"
     gene            3262..3981
                     /gene="fadR"
                     /locus_tag="OO135_001956"
     CDS             3262..3981
                     /gene="fadR"
                     /locus_tag="OO135_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5397814.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4033..5565)
                     /locus_tag="OO135_001957"
     CDS             complement(4033..5565)
                     /locus_tag="OO135_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5397815.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            5895..7193
                     /gene="dadA"
                     /locus_tag="OO135_001958"
     CDS             5895..7193
                     /gene="dadA"
                     /locus_tag="OO135_001958"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5397816.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7203..8261
                     /gene="dadX"
                     /locus_tag="OO135_001959"
                     /pseudo
     CDS             7203..8261
                     /gene="dadX"
                     /locus_tag="OO135_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(8648..10384)
                     /locus_tag="OO135_001960"
     CDS             complement(8648..10384)
                     /locus_tag="OO135_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5397817.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10479..11393)
                     /gene="ldcA"
                     /locus_tag="OO135_001961"
     CDS             complement(10479..11393)
                     /gene="ldcA"
                     /locus_tag="OO135_001961"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5397818.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            11493..12104
                     /gene="emtA"
                     /locus_tag="OO135_001962"
     CDS             11493..12104
                     /gene="emtA"
                     /locus_tag="OO135_001962"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5397819.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12106..12840)
                     /locus_tag="OO135_001963"
     CDS             complement(12106..12840)
                     /locus_tag="OO135_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5397820.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQIKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13041..13295
                     /locus_tag="OO135_001964"
     CDS             13041..13295
                     /locus_tag="OO135_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5397821.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            13474..13914
                     /locus_tag="OO135_001965"
     CDS             13474..13914
                     /locus_tag="OO135_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397822.1"
                     /translation="MIQKINILDSSQSGVKNGAYSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(13993..15565)
                     /locus_tag="OO135_001966"
                     /pseudo
     CDS             complement(13993..15565)
                     /locus_tag="OO135_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
     gene            complement(15885..17303)
                     /gene="dhaM"
                     /locus_tag="OO135_001967"
     CDS             complement(15885..17303)
                     /gene="dhaM"
                     /locus_tag="OO135_001967"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5397823.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(17314..17946)
                     /gene="dhaL"
                     /locus_tag="OO135_001968"
     CDS             complement(17314..17946)
                     /gene="dhaL"
                     /locus_tag="OO135_001968"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5397824.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(17918..19027)
                     /gene="dhaK"
                     /locus_tag="OO135_001969"
     CDS             complement(17918..19027)
                     /gene="dhaK"
                     /locus_tag="OO135_001969"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5397825.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGKLGESNVTEQNSNC"
     gene            19255..21174
                     /gene="dhaR"
                     /locus_tag="OO135_001970"
     CDS             19255..21174
                     /gene="dhaR"
                     /locus_tag="OO135_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5397826.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSAFLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
BASE COUNT         5328 a         5324 c         5526 g         5132 t
ORIGIN      
        1 gatttatcaa atctggaacg ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc
       61 tgggggattg gacggcggat cagcaaaaaa ctggacgcga tggggatcaa aaccgttctt
      121 gatttggcgg atacagatat ccggtttatc cgtaaacatt ttaatgtcgt gctcgaaaga
      181 acggtgcgtg aactgcgcgg tgaaccctgt ttgcaactgg aagaatttgc accgacaaag
      241 caggaaatta tctgttcccg ctcgtttggt gaacgtatca cggattatac gtcgatgcgg
      301 caggccattt gtagttacgc tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat
      361 tgtcggttta tctccacatt tattaagacg tcaccatttg cgctcaatga accttattac
      421 ggcaatagcg cgtcggtaaa actgctgacg cccactcagg acagcaggga tatcattaac
      481 gccgctacgc gatctctgga tgccatctgg caagcgggcc atcgctacca aaaagcgggc
      541 gtgatgctgg gggatttctt cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac
      601 gcaccgcgcc ccgggagtga gcaattgatg acggtaatgg atacactgaa tgctaaagag
      661 ggcagaggaa cactctattt tgccgggcag gggatccaac aacaatggca gatgaagcga
      721 gccatgcttt caccacgtta tacaacgcga agttctgatt tactgcgggt caaataaata
      781 tagcggcagg aaaaaagcgc tcccgcagga gcgccgaatg gattagcgac cgaacagatc
      841 acgttttttc gctttaaacg gctgggaaat catcaccagc accgcgacaa tcaggtaagc
      901 gataaaaata ccgagcagcc actgcggcat ttccagacct aaaaaatccc actgacgctc
      961 ggcgcaatcg ccagaggcga caaacacttg cggcacccat ttatccagcg gcagccattc
     1021 cgggaaacga accataaaat cacaggtggc aaacggcgaa ggatagagct gaagcatggt
     1081 gtgctcgtaa gttaactgca caccgcggaa cgcactatac aaccagataa ccatcgctac
     1141 ataacgcagc ggagttttcg gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa
     1201 taacgcgcag cgttcataaa tacagagcac gcaaggtttc agtaacatca catgctggaa
     1261 ccacagcgcc gtcagttcca gtgccagagc agtaaacgcc atcaacagcc acgcgccccg
     1321 gccttgtgaa cattggttca aaaatcgcaa cataatcatt tccctgcaat atgcatagag
     1381 tgcgcagttt aaaccaattc attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa
     1441 ttggacaatt atcgggcgaa aaggcaaacc gggcatttat atgcccggta agttgttatc
     1501 aaagcgttgc tatccagcgc atttgcataa accattcggt tacaggggca agcgtaaact
     1561 cgacgcagag cagcccgacg agtgtcagga cgagggtgta aggcagggcc atccacacca
     1621 tgcggccata agagaggcga atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg
     1681 cagcctgacc gttcggcgta gcgacagagg gcagattggt accggtatta atggcgaccg
     1741 ccagcagctc gtattgcttc aacgtgatag caccactttc cattgccgct ttcgcttcgt
     1801 tgatataaat cgtccccacg aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa
     1861 tatagaacaa cgacagctga gcatgttccg atgcctgcaa cacaaactga ataatcggcg
     1921 aaaacagttg ttggtcgata atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg
     1981 tgaatggcag agattcggtg aaggctttac cgatagcatg ttcatcggtg acaccggtca
     2041 atgatgtcgc cagaataatg accgacaaac caatcaagcc aacctccgcc agatgcaacg
     2101 ccagcgcagt caccagccag acgccgataa tcgcctggac aatcagacgg attttatcct
     2161 gacgggtacg ctggtggcgg ctttgatcgt caaactgttg caacacttcg cggactttct
     2221 ccggcagcgt ttcaccgtag ccaaaccaac gcagcttctc tatcagcagg caggttaaca
     2281 ggccacaaat cagaaccgga acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat
     2341 gccagccagc cgctttagcg atgatcaggt tctgtggttc gcccaccatg gtcattacgc
     2401 cgcctaatgc ggtgccgaca ccggcatgca tcatcaggct acgcagaaag ccacggaact
     2461 gttccagaac cactttgtaa tgcttgtcga tatgactatc gtcttgcagg tcggtatctt
     2521 cagtacggga agaggctacg cggtgataaa taccataaaa accgactgca acgctgatca
     2581 ccaccgccac gacggttaag gcatcgagga acgcggagag gaacgccgcc gccacgcaaa
     2641 aagagagcga cagcagcatt ttggaacgaa tgcttaacag caaacgggta aatatgaaca
     2701 gcaatagctg tttcataaaa tagatacccg ccaccataaa catcagtaac agcaagactt
     2761 caagatttgc cgccacctct tcacggacgt gttccgcgct ggtcatgccg atgaataccg
     2821 cttcaatagc caacagacca ccggggagta gcgggtagca tttcagggcc atcgccagag
     2881 tgaaaataaa ttccgctacc agcaaccagc cagcgacgaa agggctgatg aggaaaatta
     2941 acggatttac gattaagaaa atgatgaggg cgagtttgta ccagtcgggg gactggccca
     3001 aaaaattgcg ccatagcgcg cggccccagg agatctccat gatggtttcc cttaccttac
     3061 aaataatcaa tgatgttttt atgtttaaac gcaaagctta acggtcaggc aggagtgagg
     3121 caagtcttga tagtcaaggg gaaagagatg cggaaaatga agccttgatc ccttttttct
     3181 tctttttgtc tgctatcagc gtagttagcc ctctggtatg atgagtccaa ctttgttttg
     3241 ctgtgttatg gaaatctcac tatggtcatt aaggcgcaaa gcccggcggg tttcgcggaa
     3301 gagtacatta ttgaaagtat ctggaataac cgcttccctc ccgggactat tttgcccgca
     3361 gaacgtgaac tttcagaatt aattggcgta acgcgtacta cgttacgtga agtgttacag
     3421 cgtctggcac gagatggctg gttgaccatt caacatggca agccgacgaa ggtgaataat
     3481 ttctgggaaa cctctggttt aaatatcctt gaaacactgg cgcgactgga tcacgaaagt
     3541 gtgccgcagc ttattgataa tttgctgtcg gtgcgtacca atatttccac tatttttatt
     3601 cgcaccgcgt ttcgtcagca tcccgataaa gcgcaggaag tgctggctac cgctaatgaa
     3661 gtggccgatc acgccgatgc ctttgccgag ctggattaca acatattccg cggcctggcg
     3721 tttgcttccg gcaacccgat ttacggtctg atccttaacg ggatgaaagg gctgtatacg
     3781 cgcattggtc gtcactattt cgccaatccg gaagcgcgca gtctggcgct gggcttctac
     3841 cacaaactgt cggcgttgtg cagtgaaggc gcgcacgatc aggtgtacga aacagtgcgt
     3901 cgctatgggc atgagagtgg cgagatttgg caccggatgc agaaaaatct gccgggtgat
     3961 ttagccattc aggggcgata atcccttccg tttaaaaagc aaacccctca aacgaggggt
     4021 tttttgttgt ttttacagat ttcccattct tggcgggcaa cgttccagca actcgacgct
     4081 gccatcttcg ttttgctgtt ccagcatcac atcaaatccc cacaggcgat gcacatgctt
     4141 cagcacttct ttgcgccccc gatccagcgg tgcgcgatta tgtggaatgt aacgcagcgt
     4201 cagcgaacgg tcgccgcgca aatccacgtt ccagatctga atattcggct ccagattact
     4261 taagttatat tgcgacgata accggttacg gatctcccga taaccttctt cattatgaat
     4321 agcggaaatc tccagataat tatgccgatc gtcatccagc acggtgaaga agcggaaatc
     4381 acgcatcact ttcggtgaca gaaactggct gataaaactc tcatctttga aatcacgcat
     4441 cgcgaaatgc agcgtttcca gccagtcgga accggcgata tccgggaacc agtatttgtc
     4501 ttcttccgtt ggcgactgac aaatccgttt aatatcctgg aacatggcga acccgagggc
     4561 atacgggttg atgccgctgt accacgggct gttatagggg ggctggaaga ccacattggt
     4621 gtggctgtgc aaaaactcca gcataaaacg ttccgttact ttcccttcat catacagatg
     4681 gttaaggatg gtgtagtgcc agaaggtggc ccagccttcg ttcatcacct gagtctgttt
     4741 ttgcggataa aaatactggc tcaccttacg cacaatacgc aggatttcac gctgccatga
     4801 ttccagcagt ggcgcatttt tctccataaa atagagcagg ttttcttgtg gttcggacgg
     4861 atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag
     4921 catattgacc tgactttgta gatactcttc gcgacttttc tgccgggctt tctcttcttg
     4981 cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg tagttcatca gcgcgtggca
     5041 cgagtccaga agccgttcta cttcatccac gccataacgt tcttcgcact cggtaatata
     5101 tttacgggca aaaatcagat aatcgacaat cgaactggcg tcggtccaac tgcggaataa
     5161 gtaattgttt ttaaagaaag agttatgccc atagcaggcg tgtgccatca ccagcgcttg
     5221 catggtaatg gtgttctctt ccatcaggta agcgatacac gggttagagt taatgacgat
     5281 ttcataggcc agtccttgct gaccgtgctt atacagccgt tcagtctcga taaacttttt
     5341 accgaatgac cagtgcggat agttaattgg catgccgacg ctggagtagg catccatcat
     5401 ctgttctgag gttatcactt caatctggtg cgggtaggta tccagccggt agagtttcgc
     5461 cacccggtct atctctgcca gataaacatc cagcaggtcg aacgtccagt cgggtccatc
     5521 gctcaaccgt gtggtgtcct tattcataga atcgatagtc gccatacgcg cacctcattg
     5581 ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat agaacacctg ttaaaaaccg
     5641 cgtcgccgga gaattttttt ctttgcgatt tcttattatc agagtgccac taatccgctt
     5701 ctgaacggaa ttttatgctg gataaaaagg gcgttcagca ggagatacta aagacgccat
     5761 attgccgcag agtcagggag atgtgagcca gctcaccata aaaaagctgt atgttgaata
     5821 atattttcaa ctgagttatc aagatgtaat tagattatta ttcttttact gtatctaccg
     5881 ttatcggagt ggctatgcga gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg
     5941 cctggtactt aaatcaggca ggacatgagg tcaccgtcat tgatcgggag ccgggggccg
     6001 ccctggaaac cagcgccgcg aatgccgggc aaatctcccc cggatatgct gcaccgtggg
     6061 cggcaccagg tgtgccttta aaagcgatca aatggatgtt ccagcgccat gcgccgctgg
     6121 cggttcgtct cgacggtacg cagtttcagt tgaaatggat gtggcaaatg ttacgtaact
     6181 gcgacaccag ccactacatg gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc
     6241 gtgattgcct gaaagcattg cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg
     6301 ggacgctgca actgttccgt accgaacaac agtatgaaaa tgcgacccgc gatatcgccg
     6361 tgctggaaga tgccggcgta ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg
     6421 agccagcgct ggcagaagta gcgcacaaac tgacgggtgg tctgcaactg cccaatgatg
     6481 aaaccggaga ctgccagctg tttacccaga atctggcgcg gatggcagag caggcggggg
     6541 ttaaattccg ctttaatacg cccgttgacc aactgctttg cgacggcgag caaatctacg
     6601 gcgtgaagtg tggcgatgaa gtgattaagg ccgatgcgta tgtgatggcg tttggttctt
     6661 actcgacggc gatgctcaaa ggcattgttg atattccggt gtatccgctg aaaggctact
     6721 cgctgactat tccaattgcg caggaagatg gtgcgccagt atccaccatt cttgatgaaa
     6781 cctacaaaat cgccattacc cgtttcgata accgcattcg tgttggcgga atggcggaaa
     6841 ttgttggttt taacaccgag ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc
     6901 gcgatctcta tccacgcggc ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc
     6961 cgatgacgcc agacggtacg ccggttgtcg ggcgtacacg ctttaaaaat ctgtggctga
     7021 ataccggtca cggcacgctc ggctggacga tggcttgcgg ttccggtcag ttgttaagcg
     7081 atctgctctc tggtcgcacg ccagcgatcc catatgagga tctaagcgta gcgcgctaca
     7141 gccgtggatt tacgccatca cgtccgggcc atttacatgg cgcacacagc taaggaaacg
     7201 agatgacccg tccgatacag gccagcctcg atctgcaggc attaaaacag aatctgtcca
     7261 ttgtccgcca ggccgcgccg cacgcgggtg gtaaaagcga acgcttacgg gcatggtatt
     7321 gagcgtatct ggagcgcgct cggggccacc gatggctttg cattacttaa cttggaagag
     7381 gcaataacgt tacgtgagcg cggctggaag gggccgatcc tgatgctgga aggatttttc
     7441 catgctcagg atctggatat ttatgaccag caccgcctga ccacctgcgt acacagcaac
     7501 tggcagctca aagcactgca aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa
     7561 gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc gcgtgcttac cgtctggcag
     7621 cagttgcggg caatggcgaa tgttggcgag atgaccctga tgtcgcattt tgccgaagcg
     7681 gaacatcctg atggaatttc cagcgcgatg gcgcgtattg agcaggcggg gaagggctgg
     7741 agtgtcggcg ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg
     7801 actgggttcg gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata
     7861 tcgccaatac cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga
     7921 cgctaaaagc gggtgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc
     7981 gaatcggcat tgtcgccgca gggtacgccg acggttatcc gcgccacgcg cctaccggta
     8041 cccctgtttt agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc
     8101 tggcggtcga tttaacgcct tgcccgcagg cgggtattgg tacgccggtt gagctgtggg
     8161 gcaaggagat caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga
     8221 tgtgcgcgct ggcgctacgc gtcccggttg tgacggtgta acttgttgta agccggatcg
     8281 gaggcaacgt cttctgggtg caaaaaaaat catccatccg gctggtcagc aactgtagtt
     8341 gttaatgtga cagagccatt gcccatgata gtgtctatta aaaggatgga cactatttcc
     8401 ccggaacctg aactcaccgc acaggcgttc tacataaaac gcttacgctt cattgttgac
     8461 tcggagtcga cttcagataa atcacgcttt acccttgatg gagcctgtac atagatttgt
     8521 gtaattgcct gattttgata tgttcaattc aacatcaaat gaaggttaaa ttatggacga
     8581 caaacaattg caggctcagg ctgcgttcag caaagcatcg caaccggcga tagatgcttc
     8641 attaaattta agattcagct tcctcttcag ccacccgtac gccaatcttc aacacttcat
     8701 tatcttcttt ctcggccacc gtccagatca tcccggcaaa ctctacctgg tcaccgacaa
     8761 ccggtgccgc gcctaacaac tgctggacaa tttcacccag cgtttgctgc ttatcacgat
     8821 attctcgccc gtcttccagg ccatatatca gcgcaacatc agcatatttg gcgctggctt
     8881 cgagaatgaa gtcaccaaag aagcgttgat ccagcgcgac cggcggcgac tggctgaaca
     8941 gtttaccgag cgccggaaga tcgcgttccc gaccaattac acacaacaca tcgccttcgc
     9001 gcagtcgggt gctgccggta ggatgaagca actggttatc acgaaacagt gccgcaatac
     9061 gcgtctcttt tggcatatgc aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac
     9121 tcaattgata aacaaactgc tcccacggat tttccggatg aatatccagg ccaacgcgtg
     9181 acaccggacg tcccactggc ggaaccacca ctttggcttt tttagccgcc cacgagagtg
     9241 atgttccctg caagagcagt gaaaccagaa ccacaaagaa ggcgacatta aagaacagac
     9301 gtgcattctc cagccccgcc atcatcggga acacggccag gatgattggc accgcgccgc
     9361 gtaatcctac ccagctgata aacacgcgct cacgcagatt gaaaccgcgg aaggggagca
     9421 atccagcaaa taccgaaagg ggacgggcga agaatatcat ccatgccgac aaaatgagcg
     9481 ccggaatggc aataggcagc agatcgcttg ggttaaccaa tagccccagc accaggaaca
     9541 tggcgatttg cgccagccag gcgaggccgt cgaaattttg caggatgccg tagcggttgc
     9601 gaatcggccg attacccagc agaaaaccgc acagataaac agccagaata ccgctgcctt
     9661 ccagcgccgt ggttaaagca aaaattaaaa taccgccgct taatgccagc aatggatata
     9721 atccggcagg cagggcgatg cgattaatca tttgcagcag taaataacca ccgccaagtc
     9781 cgatgacaat cccgaggcca aattgctgca gaatatcgac cacgaacatc caactgacac
     9841 tgctttcatg ttgctggatc atcgcaatca gggtaatcgt cagaaagacc gccattggat
     9901 cattactgcc ggattcaatt tccagcgtcg agccaacacg ttcgttaagc cccttaccac
     9961 ccagcagaga aaagaccgct gcagcatcgg tagagccgac gatagcgccg attaataagc
    10021 cttcaatcaa atcaagatta aacagccacg ccgccatcat gccggttaaa ccagaggtga
    10081 taagcacgcc cagcgtcgcc agcgacagtg ccggtcctaa cgccacacga aaggaactgg
    10141 cctgagtgcg catcccgccg tcgagcaaaa taatcgccag tgccaggtta cttaccatgt
    10201 aggcgaaggg gtaattatca aacgggatac cgccgacgcc atcgactcct gccagcatgc
    10261 cgatcgccag aaaaataacc agaataggaa tgccaagacg ggaagaaaat gaactaagta
    10321 atatactgct ggtgacaagg atggatccta agatgaaaag gctaattatt gttgtggcat
    10381 ccaacggtcg gttactcctg attacgctgt ctcttatata aaccctacca tattagcggc
    10441 agagacagcg ttttacttag ccctgaagcg tgattttttt acattttaag aacaggatga
    10501 ccgctaatcg tcaactgagt gccttcctgg gtgttattca gaatggcatg tgcgcccaac
    10561 ggcagcgtaa ccgtgcgttg ttcatgacca aaatcgaggc cggtaatgag tggaatcgac
    10621 aggcgggaac gcaaaaacgc gtacactgac tccaggttgt aacctgcgtc ataatcattg
    10681 ggcgtgctgc cgctaaagct accgagaata atcgccttct gacgtggcaa aattcccgca
    10741 tgatagagct gcaacagcat acgttcgacg cggaaagggt gctcgttaat atcttccagt
    10801 accaggatac cgttctcaat ttttggcatc cacggtgtac caatcagtga aatcagcatc
    10861 gcaagattgc ctccccacaa cgtgccttcg gtctgacatg tcgggccttc cccttgccat
    10921 tcaatggtga aggttttatt gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc
    10981 agttcatccg caccaaagtt tgctaccagc atggggccgc taaaggtgat gacattgcct
    11041 tgcgccagaa gaccgcactg aatggcggta aaatcgctat gtccgcaaat gagtaacggg
    11101 tcatgttgtt ggcgagccac cagtgcctgc cagtcaatat ctgccagtaa acgactggca
    11161 ccgtaaccgc cgcgtacagc cagcacgatg gtgttgggtg tagtcagtct ggcaagggaa
    11221 ttgagatctt ccagacgctc cgtttccgta cccgcaaaac gctcacaacg acgggcaata
    11281 acctcgacgt tattgacttg atgccccgcg tcggtaaggc gctggatacc acgcagcgcg
    11341 gcgtgctgtt taatgctgta acccgatggg gcaattaagt gaaacagaga catggcaatt
    11401 ccttgctgac aacagaaacg aaatgtatat catgccgctt aggtgtgccg ttgtcacctc
    11461 aacggcgatt ccaggctata aggatagaag aagtgaaatt gagatggttt gcctttttga
    11521 ttgtgttatt agcgggttgt tcatcaaagc atgactatac gaacccgccg tggaacgcga
    11581 aagttccggt gcaacgtgcg atgcagtgga tgccaataag ccagaaagcc ggtgcagcct
    11641 ggggcgtcga tccacaattg atcacggcga ttatcgctat cgaatcgggt ggtaatccga
    11701 acgcggtgag taaatcgaat gccattggtt tgatgcagtt aaaagcttca acctccggac
    11761 gtgatgttta tcgccgtatg ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc
    11821 cagagcgtaa tatttcaatg ggggcggctt acctgaatat tctggaaacc ggcccgctgg
    11881 caggcattga agatccgaag gtactgcaat atgcgctggt ggtgtcatac gctaacgggg
    11941 caggtgcgct gctacggact ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg
    12001 atttggatgc tgacgagttc ctcgagcacg tagcgcgcaa tcatcctgcg ccgcaggctc
    12061 cgcgctatat ctacaaactt gagcaggcac tggacgcgat gtaaatcagt cgcgtacttt
    12121 gtccgctttc tcccgggctt cccgctcgag agagaaaata atccgttgta attgccgctc
    12181 caccgtcggg ctgacgttaa gaaaacggaa gctcagacgg ggagtggtga tggtttcatt
    12241 cttgccatca atcactttgc gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac
    12301 accccattgc cccatgttga cttcaatctg agcgaagcgc atgccttctt gtaattcggc
    12361 aggctttgct gtttccagta atgcgcccat gccgcctaac gacaaatcat acaggcggaa
    12421 acgtaacgta ctgttatccg ccagtttgat ctggcaaaaa taaggcggat ggagtggggc
    12481 ggagatgcgg aaatatcggc gtcgttgtac aaaccataag gtgggaggcg gtacggtaat
    12541 aaatgccgga agctgcaagt attcactctg ctgtagttgt tcaacagtaa actcgacttt
    12601 cgcaccctga gtttcggcgg taatggtaat atgctgcgcc tttagcacgg cgatgttgtc
    12661 ttcggcttga ctgccgaaat ccagcaccag tttatccggg gttattgcca gtattttgct
    12721 gatcagctgc ccgccattcc aactgagacg caaaggaatt gcggctttgt gcaaatcgcg
    12781 taacacgccc aggacggcta acggattttg tttcaggaac tgctcatggt aatgactcac
    12841 gcggaaaaac tcctgactga caaactgtct ttggtttatc ggtcacagtt aacaaaactt
    12901 aatacaaatg cgtgaatatt ttttacatgt tgttcttaaa tcagccgaca tacgcccagc
    12961 atcgatcccg accttgccta tacttagagc gttgacgtaa gcatttcttg cgtccgattc
    13021 atcgaaacga gggcctgaac atgggaatta ttgcctggat tatttttggc ctgatagccg
    13081 gcattatcgc caagctaatc atgccggggc gtgatggtgg tggatttttc cagacctgta
    13141 ttctcgggat agtcggtgcg gtggtcggcg gctggctggc gaccatgttt ggcattggcg
    13201 gctccatcag cggttttaat ttgcacagct tcctggtggc ggtggtggga gctattctcg
    13261 ttctgggcgt attccgcctg ctgcgaagag aataagattt tcataaggcg gatagcgata
    13321 cagatgccgc tatccgcttt tcacatcaga acgtatattc gacacctgct ttacgggtga
    13381 aaaaaatcaa tatagcactt cgatatccga taaagtcggt tcccggtaac aacaacagaa
    13441 tcctttttta attattgttt cgttgttttt ggtgtgattc agaaaataaa tattttagac
    13501 agtagccagt caggcgttaa aaatggggcg tatagtgagg cggttttggc tgcgcttatt
    13561 gaacatttag aattgatcaa tccttctggc agattaagcc aaaatacccg ttcggcaatg
    13621 cttcagttac gcgaagagga atggtctgaa ttcttttttt ggttgctaaa ttctctggag
    13681 tgtctggatt atgtgattat taatttaact ccagaaagca aaaaaacgtt gatgagtgaa
    13741 caccgtaaca atattcaggt agcaatcgat gctttatata ggcaacgtcg gtgtaaatca
    13801 ccgggggatg atagtgaaac acttacccgt agaaatgacg ctatcttcgg caaccacgta
    13861 tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaca
    13921 ccatagctat ggtgttatct gccgacaacg ccatccggag aagccgaatg acgttgtcgg
    13981 agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt gagggttgcg tcgttgctga
    14041 cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg ggcagatcaa
    14101 atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc
    14161 gccacctctt tttgcccgta gttttgtaat ccttctgtcg cgacccactg taacggtgcc
    14221 cagccatttg gcgcatccca ttgttgccca cttttcaccg acgtggtgtt caggccgccg
    14281 ggttgcagca gatgtgtttt cgtcgccgtc gccattttgt tggcgcgatc tttcgctgcc
    14341 gcattgacgt acagcgggaa cagggcggcc gcggttaact gattgcgcac tttatgactt
    14401 ttcaggtcgt aatcggcata ccagccttgt tgatcgttcc acaggtattt ttcgattcct
    14461 ttttgacggg cattcgccag cgtttcgtac tggtttgcca tcgcgttatc tcctgcagct
    14521 ttgctggcgc gggcgaggat tttttccatt ttaaacatca ggctgttcag atcgaccggt
    14581 acgatgctgg tggtgcgtaa ggtatttaac tgctgcgggt tatccatcca gcgcgagctg
    14641 aaatcccagc cagacgcagc ggcagagcgc aggtcgcggt aaatttcagt ggcaggtcga
    14701 ttcggattgc ttttggcggt ggcaatatct tccacccatg actctggtcg tggcgtatcg
    14761 cgatcgtccc agtagcggtt gagaagggta ccatcctgaa gtttgacaac gcgtttttcc
    14821 tgttgtccgg cttgcaggtt ttcaacaccg tccatccagt aagcatattc tttttgcatt
    14881 tgcggcaggt attgcttcaa cgcggcatcg ccttcatgct gcgccagtaa ctctaccatc
    14941 agggcaaaga agggcggttg cgagcggctt aaatagtaac tgcggttgcc gttgggaata
    15001 tgaccgtaag tgtctatttc atgagcaaaa ttggccacca tatccgcgac tttatcccag
    15061 tgaccgcttt cggcaagtcc taacatggtg aagtaactgt cccagtaata tacctcgcga
    15121 aaacgtccgc ccggcacgac ataaggttcc ggcagcggta acagagaatc ccatttttcg
    15181 gtgttttcgg tagaacgcgt taataccggc caaagtccgt caatatgttc gcgcagtgac
    15241 tgcccctctg gcggaacata tttctcgcct tctttcggca gggtgaaatt gacgttaacg
    15301 aaatggcgca gatcaaatcc gctctggttt tgctgcatcc gataatcagc ggatcgctgt
    15361 tcggcacggc atcggcaaag gttttttggt ccggaaaaag tttggcgttt tgcacatcat
    15421 taaacagcgg ccctaataaa atatcaggcg gctgtggtgt taccggtgtt tcttctgcct
    15481 gcaccgatag cgcagcgaaa cacaaaaaga tacaggctgg aattaacgcc attttttgcg
    15541 ggcgagaagg tgcgggggat ttcatcaatc attctccttt ggcgaaaccg aataaaacgg
    15601 tattcaagta tcagaaaacc ttagttcagg atcgcgctag ctgcatgatc gaaatctcat
    15661 tctgcgaact gtcaggcatt ttagaaaagg gaattaaagg gataaataac ggcgaaatag
    15721 agtgatcaaa taacgtctgt ttattagcca gccagcggcg ttgggttcgt gattagcgaa
    15781 gaaaaatcat cataaattgg tgaaaaatat aacaggtagt taaaaaccat tagtgctgag
    15841 taaattgccg gatgacatca gaacgatgcc atccgaacag tggcttaacc ctgacggttg
    15901 aaacgttgcg ttttaacgtc cagcgttagc gtttcttctg gttgtatcgc atacagtttc
    15961 tcaccctgct ggcaaatcca gccaatcccc agttcacggg cgattagggc gctgtgggat
    16021 accggacttc cggcgctaag gcagatacct tttacaaccg ccggatccag ttgcagtact
    16081 gtggaaggat aaatgttctc cgccagtaga atagttggcg agttaaactg cgggagttct
    16141 tctttcgttt gggtcaggtg gaccagggtg cgatgcagaa gatcgtccac atcaatatag
    16201 cgagcttgta gatattcatc atccagttgc tggtattgct ggctaagttc tttaagaact
    16261 tgctgccagg catattctgc cgtgcaatgt tcatgctgaa ggagttcgct tgccgccgcc
    16321 agcagttccg gatcatctaa cagtgtatgg tgaccagaaa agattgcggc aatatcgtca
    16381 agcccgctgg cttctgcttt cgctgttaac gtcatcagat ctaataacgt gaagtcaata
    16441 gcctggcgta atcgatcttg ttcttcttcc acggtcaggg ttgattttgc ctgtaccgta
    16501 tataaaactg gttgataata aaaggcttta cccgaaacag gcggaacggg acgcagagta
    16561 ggtggagcga cttcctccgt ttcaccaaag ttatcttcag ccagctgacg gaaagcgatc
    16621 agtgcctctt cagcttctgg ccctttcgca atcaggcgca gcgtatcgtt atagcgaact
    16681 tgtagtaacg caatctggtt aatactctct ggtgtgacgc atttgccgtt tttttccagc
    16741 aacatatcgg cattaaatgt cgataaggta taaaccagcc gggaggccgg acgtacatgc
    16801 aggccgttac ggttttttat gaccaccgcc agagaacggg cttcttcatc gtacgcagga
    16861 catgtgtcag agatttcagt gtcggaggac ggtaaaccca gttgttcacg tttggcttcc
    16921 agcgcatgca tagcgtcaaa gataactttg tcgatatccg ctcccgaggc cgcgctgacc
    16981 gttgctgcca gtgtaccttc gaccaacggc gcagcacaca aacgtacttt tgcggcgatc
    17041 ttgggagcca gcaattccag cgcagtttca gcactcaata atgcgctacc catatccatc
    17101 atgaccagca catggtcggc atcagcaaca gattcgatgg cctccatcac tttgacggca
    17161 tcggtaccaa taggattttg tggatcgtca attcccgcgg caatggcgat tttacaacta
    17221 tcactcatta acatctgacg ggctaattca ccgacacctt ctcccagtcg gctgctatgt
    17281 gaaactatga ccaggtttac catcaccaat tccttactct tttgcggcta acgccaacat
    17341 ttgcatcata aacatcaccg aggtcgcgcc gggatcctgg tgaccaatac tgcgttcacc
    17401 gagataactg gcgcggcctt tgcgggcttg catcgtaatc gtactttgtg cagcggattc
    17461 ggcgatgcta ctggcagctt cgagcgccac cggaacagag agattttgct cgctggactg
    17521 acgtaacgat tccaccaccg gcacccacac atcacacatg gttttatcgc caggttcggc
    17581 tttcccgcga ctgattacgc cgtccgcgcc atcgcggaac atctgataaa gctcttccag
    17641 tgtcaggctt tgccgtgcct gggtcgcctg tgcggcgcgg ataaagaagg taccgaacag
    17701 cggaccactg gcaccaccga cgctggaaag cagcgtcata ccggtattct tgagaatgaa
    17761 accgatatct ttatctgcga tagcagggag tttttccacc actttgctaa agcctcggtt
    17821 catatttagc ccgtggtcag cgtcgccaat ttcgcgatcc agtccggtaa gatactcgct
    17881 ctcggtgctg aaaatatcgc cacaacgagt gagccagtta acaatttgag ttctgctcag
    17941 tgacattgct ttctcctaat ttaccccagt taagggccgg ggtgtggacc ggggcgtccc
    18001 agagtgccag cgtttcgtca tcaactttca gtaaggtgat tgagaaaccg gtcatatcca
    18061 gtgaggtgca gtacgcgcca attaaattac gttcgatagt caatcccgct tgctggcaac
    18121 gtgtggtcag gcggttatag acgccgtaca gctcagaaag cggagttgcg ccaagattgt
    18181 taaccagcgc aatcacccga tcgccagact ggagcggttg tttggtttgt tgttcttcct
    18241 gccaactgcc ttgttgataa tcccagaaac gtaaagtgcg atggtatgag ccatttccca
    18301 gcagggtgtc gaacatttca tcgacggttt gatcaaggga agagaaggag cggcggtcaa
    18361 tacccggctc accatgaatg ccgacgccaa actccatctc attatccgcc agggtaaaag
    18421 aaggtttgcc cgcggcagga acggtacagg caccgagagc gatacctatt gagtggcctt
    18481 gattattcaa cttacgcccc agttccgcac aggcgtccag tgagtcgcca cgctccgccg
    18541 ctgcgcctac gagtttttca attaatacgg tgttggcaac gccgcgtcgc ccggcagtat
    18601 aaagactgtc ttttaccgca acgtcgtcat caatgaccac agtggtcact tttacgccgc
    18661 tatcgtgcag taactcggtc gctgtttcaa agttaagaat atcgccggtg taatttttga
    18721 taatcaacag tacaccttcg ccgccatcaa cttgcatggc gcattcaaag attttatcgg
    18781 gcgtcggtga ggtgaaaatt tcgcccggac aggcccccga aagcatcccc tgaccgatat
    18841 acccacagtg catcggctcg tgtccgctgc cgccacccga cagcagggcg acttttcctg
    18901 caacaggggc atcagctcgg gtgacataca ccggatcctg atgcagtgtc agcgatggat
    18961 gcgctttcgc cagtcctgcc agttgttcgt ccagtacgtc ttgcacatca ttgatcaatt
    19021 ttttcattat tttgctccag caattacggt agggcatgga tgatgttcaa cgacacggcg
    19081 accactgact gccgatgaat ccattgtgca tcaggcaagg gaaaagaaaa ttagcccaaa
    19141 ttatgtttca tagtgaaaca tatgctttaa tgaatgttcc atattgaaac ttttacgtgt
    19201 attaatactt aaaattgcga gccggaacac cttttgtcat aagggatgcg ggatatgagt
    19261 ggcgctttta acaacgatgg tcggggcata tctcccttaa ttgcaacctc ctgggagcga
    19321 tgcaataagc tgatgaaacg ggagacatgg agcgtaccac atcaggccca gggcgtgaca
    19381 tttgcttcta tttatcggcg taagaaagcg atgctgacgc tcgggcaggc tgcgctggaa
    19441 gatgcctggg aatatatggc accgcgagag tgtgcgctgt ttatcctcga tgaaaccgcc
    19501 tgcattctca gccgtaatgg cgatccgcaa accttgcagc agctaagtgc acttggattc
    19561 catgacggca cgtattgcgc cgagggaatt attggtactt gtgcgctatc gttagcggct
    19621 atctctggtc aggccgtgaa aacgatggcc gatcaacatt tcaaacaggc actctggaac
    19681 tgggcctttt gtgcaacgcc gttgtttgac agcaagggcc gattgacggg aacaatagcg
    19741 ctggcgtgtc cggttgagca aactaccgca gctgatttgc cgttgacgtt ggcaatcgcc
    19801 cgcgaggtcg gaaatttact gctgacggac agtttgctcg ctgaaactaa ccgtcattta
    19861 aatcaactta atgccctgtt agaaagtatg gatgatggcg tgattagctg ggacgagcag
    19921 ggtaatttgc aatttatcaa tgcccaggcg gcgcgggtct tgcgccttga cgcgacggca
    19981 agtcagggac gggcaatcac tgaactctta acgttacccg ccgtattgca acaagcaata
    20041 aaacaggcac atccgctcaa acacgtagaa gcaacctttg aaagtcagca tcagtttatt
    20101 gatgcggtga taacccttaa accgataata gaaacgcagg gaaccagctt tattttgttg
    20161 ctccatcctg tggaacagat gcggcagttg atgaccagtc aattaggaaa agtcagccat
    20221 acctttgctc atatgccaca ggacgatccg caaacccgcc gcttgattca ttttggtcgc
    20281 caggcggcgc gcagtagctt tcctgtcctg ctttgtggag aagagggcgt gggcaaggca
    20341 ctgctaagtc aggcaattca taatgaaagc gagcgtgctg caggtcctta tatcgccgtc
    20401 aattgtgagt tatatggtga tgcggcactg gcggaagaat ttattggtgg cgatcgtacg
    20461 gacaatgaaa atggccgtct gagtcggctg gaactggcgc acggcggcac gctgtttctt
    20521 gaaaagattg aatatctggc ggtggagtta cagtctgctt ttcttcaggt tatcaagcag
    20581 ggtgttatca cgcgactgga tgcgcggcgt ttaataccaa ttgatgtcaa agtgattgca
    20641 acaacgaccg cggacctcgc aatgctggtg gaacaaaatc gttttagtcg ccagctgtat
    20701 tacgcgctgc atgcatttga aattaccatc ccgcctctgc gtatgcggcg tggcagcatt
    20761 ccggcgctgg tgaataacaa attacgcagt cttgaaaaac gcttctctac gcggctgaaa
    20821 attgatgacg atgccctcgc tcgcctggtt tcttgtgcat ggccaggcaa cgattttgaa
    20881 ctttacagcg tcatcgagaa tcttgctctg agtagtgata atgggcgcat tcgcgtcagt
    20941 gatttgccgg aacatctgtt taccgagcag gcaacagatg atgtcagcgc cacccgcctt
    21001 tccaccagtc tgtcatttgc ggaagttgaa aaagaggcaa ttattaacgc agcccaggtc
    21061 acaggcggtc gcattcagga aatgtcggct ttacttggaa tcggccgcac tacgctgtgg
    21121 cggaaaatga agcaacatgg cattgatgca gggcagttta agcgccgggt atgaaagaca
    21181 gaaacgattt ctaatacatc agagtgatct atatttcatt ccggcgcacg ctaacattta
    21241 tcagcatcgt ttaagggctt gtctatcacg cacttaaaag ctgaagcgat atcctccgtt
    21301 gacttgcttc
//
LOCUS       DAJTMK010000063        20919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTMK010000063 DAJTMK010000000
VERSION     DAJTMK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20919
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.064"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(252..998)
                     /gene="cutC"
                     /locus_tag="OO135_001971"
     CDS             complement(252..998)
                     /gene="cutC"
                     /locus_tag="OO135_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5397827.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANLLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1012..1578)
                     /locus_tag="OO135_001972"
     CDS             complement(1012..1578)
                     /locus_tag="OO135_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5397828.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTAEHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1794..3527
                     /gene="argS"
                     /locus_tag="OO135_001973"
     CDS             1794..3527
                     /gene="argS"
                     /locus_tag="OO135_001973"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5397829.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3704..4192
                     /locus_tag="OO135_001974"
     CDS             3704..4192
                     /locus_tag="OO135_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5397830.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4312..4704)
                     /gene="flhe"
                     /locus_tag="OO135_001975"
     CDS             complement(4312..4704)
                     /gene="flhe"
                     /locus_tag="OO135_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5397831.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4704..6782)
                     /gene="flhA"
                     /locus_tag="OO135_001976"
                     /pseudo
     CDS             complement(4704..6782)
                     /gene="flhA"
                     /locus_tag="OO135_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(6775..7878)
                     /gene="flhB"
                     /locus_tag="OO135_001977"
                     /pseudo
     CDS             complement(6775..7878)
                     /gene="flhB"
                     /locus_tag="OO135_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(7961..8605)
                     /gene="cheZ"
                     /locus_tag="OO135_001978"
     CDS             complement(7961..8605)
                     /gene="cheZ"
                     /locus_tag="OO135_001978"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5397832.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8616..9005)
                     /gene="cheY"
                     /locus_tag="OO135_001979"
     CDS             complement(8616..9005)
                     /gene="cheY"
                     /locus_tag="OO135_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5397833.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9020..10069)
                     /gene="cheB"
                     /locus_tag="OO135_001980"
     CDS             complement(9020..10069)
                     /gene="cheB"
                     /locus_tag="OO135_001980"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5397834.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTSKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAVGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10072..10932)
                     /gene="cheR"
                     /locus_tag="OO135_001981"
     CDS             complement(10072..10932)
                     /gene="cheR"
                     /locus_tag="OO135_001981"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5397835.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10951..12481)
                     /locus_tag="OO135_001982"
                     /pseudo
     CDS             complement(10951..12481)
                     /locus_tag="OO135_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(12539..13954)
                     /locus_tag="OO135_001983"
     CDS             complement(12539..13954)
                     /locus_tag="OO135_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HCR5397836.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYKNYYTALTELIDYLDYGNTGAYFAQPTQGM
                     QNIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQ
                     ASALEETAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIAD
                     SSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSVQAA
                     KEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIVQ
                     VALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPS
                     RPASEQPPAQPRLRIAEQDPN"
     gene            complement(14099..14602)
                     /gene="cheW"
                     /locus_tag="OO135_001984"
     CDS             complement(14099..14602)
                     /gene="cheW"
                     /locus_tag="OO135_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5397837.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKEQEICGYDQ
                     VTRIANTPAFIKGVTNLRGVIVLIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14623..16294)
                     /gene="cheA"
                     /locus_tag="OO135_001985"
                     /pseudo
     CDS             complement(14623..16294)
                     /gene="cheA"
                     /locus_tag="OO135_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(16299..17225)
                     /gene="motB"
                     /locus_tag="OO135_001986"
     CDS             complement(16299..17225)
                     /gene="motB"
                     /locus_tag="OO135_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5397838.1"
                     /translation="MKNQTHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17222..18109)
                     /gene="motA"
                     /locus_tag="OO135_001987"
     CDS             complement(17222..18109)
                     /gene="motA"
                     /locus_tag="OO135_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5397839.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDCLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18236..18814)
                     /gene="flhC"
                     /locus_tag="OO135_001988"
                     /pseudo
     CDS             complement(18236..18814)
                     /gene="flhC"
                     /locus_tag="OO135_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
     gene            complement(18824..19162)
                     /gene="flhD"
                     /locus_tag="OO135_001989"
                     /pseudo
     CDS             complement(18824..19162)
                     /gene="flhD"
                     /locus_tag="OO135_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
     gene            19942..20370
                     /gene="uspC"
                     /locus_tag="OO135_001990"
     CDS             19942..20370
                     /gene="uspC"
                     /locus_tag="OO135_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5397840.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(20377..>20919)
                     /locus_tag="OO135_001991"
     CDS             complement(20377..>20919)
                     /locus_tag="OO135_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5397841.1"
                     /translation="QIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQ
                     HFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELT
                     SALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQI
                     VPRSAESQQRDKVATFPKLA"
BASE COUNT         5095 a         5673 c         5020 g         5131 t
ORIGIN      
        1 taattgctaa ggaataaccc tgttgttatt tagaatatat atttgtcagt tttagcaatt
       61 taaaccccat aattaatgtg aatatataaa atatttttag cacaacgcta tgttcactgt
      121 cacccgctca acagatcacg gttatcgtac gttttttata ctgctcaggg ataaaaaaag
      181 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
      241 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
      301 gccccgtcta cgacataacg cgaatactcg tccgcgtgtg catctgatga catggacaat
      361 ccttgattac gataacgcat cggtgaagct tgccacgctc ccgcggagct atggacttcc
      421 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccggccata
      481 atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc ttgcagtgca
      541 tctgattttt gccctgatgt cagcacccgg gcgatgccca attccgcaag attactgagt
      601 gtatttaaaa ggttagcgca catatcgaag gcgcgatgaa aagtcactgc cagcggaccg
      661 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagcacg
      721 cccgtcacca gtccaggaaa acctaattcg cggactgtgc gcacatcctc aagaatggcg
      781 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcggataat cggatgcaca
      841 gggatcgtca cccactggcg cacggatttc agtacaccca acgacggcgt taagcccccc
      901 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca
      961 cattccatgc tgtaacagca aatttccagt aatgccattt ttactcctta attacgccga
     1021 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag ttgttttgcc
     1081 atcggtgacc gccagcgtcg gattcggtaa tcgctcatac tcaccttttg gcgaactggt
     1141 tttcactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     1201 attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac cttcgtgtgg
     1261 gtaacgtttt tccccgggcc acggtagctc cacaatggaa aactgccagt gcgcaacctg
     1321 taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcatat tttctgacaa
     1381 aagctcgcca cactgttcaa aaccgcgacg ccagcgctca gcggtgacgt tttgatggca
     1441 acgcaaagaa atgtgctcgg cagtcagcgg agtgatatcc aaccccagac ggcgggagag
     1501 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     1561 agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa tttaccctgt
     1621 tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacggca
     1681 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     1741 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     1801 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     1861 attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat caggctaacg
     1921 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     1981 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     2041 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     2101 atcgtctcgg tgtttctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     2161 acgtggcgaa agagatgcat gtcggtcacc tgcgctctac cattattggt gacgcagcag
     2221 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     2281 gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa aacgccggtg
     2341 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     2401 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     2461 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcacct
     2521 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc ctctacaacc
     2581 caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta gaaagcgaag
     2641 gggcgaccgt cgtattcctt gatgagttta aaaacaagga aggcgaaccg atgggcgtga
     2701 tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     2761 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     2821 aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     2881 cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg ttcaaaaccc
     2941 gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa cgtgcacgcc
     3001 gtctggtggc agaaaagaac ccggatatgc cagctgacga gctggaaaaa ctggctaacg
     3061 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     3121 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     3181 cttacacgcg tgtattgtcc gtgttccgta aggcagaaat taacgaagag caactggctg
     3241 cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     3301 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     3361 tgtacgatct ggctggtctg ttctctggct tctacgagca ctgcccgatc ctcagcgcag
     3421 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg aagacgctaa
     3481 agctgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg atttttcgtg
     3541 agagtaaagc ctgatcaggg ttacctgatc aggctttttt attgccatct aaatgtattc
     3601 tgaaaatgga catgccattg ttttctgact gttggataag aggccagaag cgtaatatcc
     3661 ggccccaggg aaacgataac ggttgaattt aaggaatacc gcagtgttta aatttcttgt
     3721 attaacatta ggcattatct cttgccaggc ttacgcagaa gatacggtta tagtaaacga
     3781 ccatgacatt tcagccatca aagattgttg gcaaaaaaat tcagatgatg atactgacat
     3841 taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc aattaaataa
     3901 agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg taaaaaaacc
     3961 tgataccgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg attttaggga
     4021 caaagaatgt gaattaatcc tttcaaatga ggacgttcag gatttaagtg acccttattc
     4081 tgaatcagaa tggctctcat gtatgatcat acagaccaat acgcgaactc gccagttgca
     4141 gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat aattcacatc
     4201 tttttggcat gttttgttgc aagctattcc tgataaataa ttgcaacaag acatcgagcc
     4261 tttttcactg agttattaaa catactcgcg agcgcgtaat ttttttgtcc ttcagcgata
     4321 attcacaatc acttcattac gttgtacctt tagcggtgga attaaccgcc caccgcctgg
     4381 cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg ccacggtggt
     4441 tccgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa cccgcagtcc
     4501 tgaaggtgtc gggccgataa gctgataacg ccacgctacc agcgtcatca atcctgaagc
     4561 aggttgtcgc gtagaaagag gcgcagacga catcgactca ccgcgatgat ttagcgtaat
     4621 tcctacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca gcggaaacaa
     4681 taatattgct aataaggttc tcattatttg ccgccaattg tcgccgtcat gcggatatgt
     4741 cggttatcag acagttccag attcgacagg accactaact gcggcaagct gcggcgcagg
     4801 aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg cgcacccagc
     4861 atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc cagccctggc
     4921 tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg tgtatcgagg
     4981 ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc ccgtcccaac
     5041 gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc atgttccgcc
     5101 agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag gagattttgc
     5161 agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc cgtcagcttt
     5221 ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc aaacagctct
     5281 gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct ggcctcaacc
     5341 actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca gatagcattc
     5401 aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc ggttccaggg
     5461 ttaatcgcca accagcgccc cggataagca tcaccactgc caatctccac gcctttcatc
     5521 aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac cactggcggc
     5581 agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc caacaactca
     5641 ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat tcccagagaa
     5701 tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc tgccattttt
     5761 accggtttgg gttcggcagg cgctttttat tcgcgtccgc gtatccacca ggccagcccc
     5821 agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac caagccgagt
     5881 aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag ctgattcacc
     5941 atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgacccc cgcggcggta
     6001 gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa tagcgtataa
     6061 ctttccgccg cgtgtcccat gctcatgcca tgttgcagca cgccgaccag caacccgccg
     6121 acaacgttaa tgaccatgat gaggatcccg gcgatggcat cgccgcgaac aaacttactt
     6181 gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg gcgttttttc
     6241 gcctcatctt caccaatcaa tccagcgtta aggtcggcgt caatcgccat ctgcttaccc
     6301 ggcataccat cgagaacaaa gcgcgcaccc acttctgcga tacgcccggc acctttggta
     6361 atgaccataa agttgatgat cacgagaatg acaaacacca cgatacctat agcgaaattg
     6421 ccaccaacga ggaagtgacc gaacgcttcg accaccttcc ctgccgccgc cgcgccggta
     6481 tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg caacaacgta
     6541 gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt aaacatcgcc
     6601 accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc gagtatgaat
     6661 gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg tccggcaagg
     6721 atctgccatt gtgtcgattt caggtttgcg ggcaggcgca gcatcgcggc cagattactc
     6781 atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat gagtaggttg
     6841 tacagggcgc tgtccaccag ccagacgcca gcgtttcagt tgccagatcc aggccagcac
     6901 ttccgccacc gcggcgtaca gttgacccgg gatttgttga ccaatctccg catgtcgata
     6961 cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag cgccaatttc
     7021 acgaatgcgc agcgcgacca gccctgcacc tttagcgacc actttcggtg cgctcatttt
     7081 gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa tgacatccgc
     7141 tttcggcaca tcggccatca tccgacgccg tgcggcagct cgctgcatct gacggatccg
     7201 tcctttaaca tgggggtcgc cttcgctttg tttgaactca tcacgaatat cctgccgcga
     7261 cattcgcagc tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg
     7321 aatgacacca agcaccacca gcagtgcgca tagccctacc aaatccatcg cattacccat
     7381 ggcggtaatc ggagactcgg ccagtaagcg catcatctgc ggccagtgat gccagagaaa
     7441 aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gctttaagca actccgcgcc
     7501 agtctgtgcc gagaacatcc gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg
     7561 ctgcaaggat tttccgctaa ataccagccc ccccagcatg accggagaaa tgagcgctac
     7621 cagcaccacg ccgctaatca gcggtagcag cgccagcatg gcttctctga tcagcagaat
     7681 aatctgcctg aggatcagat tcgggtcttt gataatactg tgatcaaaat gcagcccagc
     7741 ggagagcatg cccgacaatc gacgggccag cgacacaccg ccgaaccaga taacacacac
     7801 gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt agggcttttg
     7861 ctgccacaca tcaagcatag tgtgcgtttg tcggatgcgg cgtcatcgcc ttatcagacc
     7921 gcctgatatg acgtggtcac gccacatcag gcaatacaaa tcaaaatcca agactatcca
     7981 acaaatcgtc cacctgatcc tgactggcta ccacaccggc tttgctggta tcgacctgag
     8041 gtccattaag caaactctgg ttttcacgtt ttggacgcga ctcctgttcc gggatgtttt
     8101 ccaacagcac catcagcaac tggcgttcga tctcctgaat gacatccatc atccgcttaa
     8161 taacctgccc ggtgagatcc tgaaaatcct gcgccatcat gatttccagc agttgcgcgt
     8221 tagtaaagct ggtatgcgcg ggtacatctg ccagaaattg tcgtgtatct gttaccagtt
     8281 cacgggcgtc ggcaaggtca atcggatcgg caaaccagtc atcccaacgt tgggttaacg
     8341 cttttgctga tttctccatt tgatcctgat gcggttgtga cgcctcaaca ctgttcagcg
     8401 cccgctccgc agcctgggcg gtcatctgca caacatagta caaacgatcg cgcgcatcgg
     8461 ggatggcttc cgccgcttcg gcaatggcct gatccagccc cagttcccgc aaactgtcgc
     8521 gcagcatacg cgtcaggctg ccgatgcgcg caattatatc gccagctgaa tgctcgtcag
     8581 caggtttgat tgatggttgc atcatagtcg catcctcaca tgtccagttt atcaaagatt
     8641 ttgttgagtt tttcctccag cgtcgcggcg gtaaatggct tcaccacata gccactggcc
     8701 cccgcttgcg ccgcagcaat gatgttctct ttcttcgctt ctgcagtcac cattaacact
     8761 ggcaatgccg acatcgcgcc atccgcacga attgttttca gcaattccag gccatccata
     8821 ttgggcatgt tccagtcgga gataacaaat ccataaccgc ctgcctgcaa cttattgaga
     8881 gcgtcgacgc catcttccgc ttcctcaaca ttattgaatc ccagctcttt cagcaggtta
     8941 cgcactatgc gtcgcatggt ggaaaagtca tccacaacca aaaatttaag ttctttatcc
     9001 gccatttcac actcctgatt taaatacgta tcgcctgtcc ggcactaatt tttgccaaca
     9061 tttgctggct tacctggcta agatcgacca cttcgcagac accacccata ttgatggcct
     9121 cgcgcggcat gccgaacacc acgcaacttg cttcgttttg cgcaagggtc catgcccccg
     9181 cctgacgcat cgccaacatt cccaccgcgc cgtcgttgcc cataccggtc aggatcaccc
     9241 caaccgcatt acgccccgcc tgtttggcga cagaatggaa caacacatct accgaaggcc
     9301 gatgacggtt aaccgccggg ccgtcgtgaa ttttgatttg ataatttgcg ccactacgcg
     9361 ccagttccat atgccgatcg cccggcgcaa tataggcatg ccccggcaag acacgttctc
     9421 cgtcttcggc ttctttaacc ccgatctggc aaagcttatt aagtctgtcg gcaaaagagc
     9481 gggtgaaacc gggcggcata tgttgggtaa ttaacaatgc cgggctggaa agcggcaacg
     9541 gttgcagtac gtgacgaatt gcctcagttc cacccgttga agcaccaatc gcaatcagtt
     9601 tttcagaact caacaacgga cccgccttca gcgttgtcgg tgctgacaat ggcttatgtg
     9661 ctgcaaggct cgcctttgct gccgtacgca ccttttcagc aatcatttcg ctatacgcca
     9721 gcattccttc gcgaataccc agttgcggtt tggtgacaaa atctattgcc cccagctcca
     9781 gcgcgcgcag cgtgacttct gatcctttgc tggtcaagga agaaaccatc acaacgggca
     9841 ttggacgcaa acgcattaat ttttcgagga aatccagtcc gtccatccgc ggcatttcaa
     9901 catccagcgt cagcacatcg ggattgaatt tcttaatcaa gtcacgcgcg accagcggat
     9961 caggcgcggt cgccaccatt tccatgtcgc tatggctgtt gatgatttct gtcatgatct
    10021 ggcgcatcag tgccgaatca tcgacagata acaccctgat tttgctcatc gttaatcctt
    10081 acttagcgca tacaccgtct gaccacgcag cgtgaagcgg cgctcaaggt ggctaaagtt
    10141 ttcagagtga cccgcaaaca gcaatccgtc gggtttaagg agcggaacaa agcggcgcaa
    10201 aatctcctgc tgggtatttt gatcgaagta gatcatgacg ttacgacaga agatcgcatc
    10261 aaacggcccc ggcacggtgt actgtttcgc cagtagattc agcggggcaa aatcaacata
    10321 gttcgccagc tcctgacgca cgcgtaccag cccttcatgc ggtcccgtcc ctcgcatgaa
    10381 ataccgttgc agttgctgcg gcgtcaggtt tttcaactct tcatggcgat aaataccgct
    10441 tctggctttt tccagcactt cggtgtcgat atcactggca aacactttcc agcgtccggg
    10501 cgcggtgccc aatgtgtcag ccagcgtcat cgcaatgctg tacggctctt cgccggttga
    10561 agccgccgcg ctccatacgc gatactcgcc agaacggcga cgtgcgtgat ccgcgagcag
    10621 agggaaatga tgtgcctcac ggaaaaatgc cgtcagattc gtggtcagcg aattgataaa
    10681 cgcctgccac tcaccgctgt gctgattaga ttccagcaag ttcagataat gaccgaaatc
    10741 cgtcagtccc agcgaacgca aacgacgaac cagtcggttg taaaccatgt cgcgtttatg
    10801 atcggccagg acgatcccgg ctcgttgata gatcaattga cttatccgcc gaaaatgcgc
    10861 gtcggaaagc gccaggcgct cggtcatctg taacaataaa gacgtttgcc cacagggcag
    10921 agatgaagtc atagcgcctt ctcaatcact tcaggatacc actggcgcaa tttgtaactg
    10981 cgccgactca tgtcgtgcta cttcatgttc ttcaagggta aataccgcca cacgcgacga
    11041 aagatggtcg gcctgattcg ccagttgttc cgttgccacc gctgcttctt ctaccagcga
    11101 ggcgttctgc tgagtcacct gatccatctg gctgacagcc tgtgcaacct gctcaatccc
    11161 ccgccgttgt tcttccgacg ccgaagcaat ttctcccata atgtcgttca cgcgggtcac
    11221 cgaactgaca atatcggtca tggtcgcggc ggcgttattc accagtttcg aaccctgctg
    11281 gacacgattg actgactctt cgatcagccc tttgatctct tttgctgcct gcgcgctacg
    11341 gctggcaaga ttgcgcactt cacctgccac taccgcaaaa ccacgcccct gctctccggc
    11401 gcgagccgct tccactgccg cattcagggc cagaatattg gtctggaaag caattccgtc
    11461 gataacgctg ataatgtcgc caattttttg cgagctggtg gcgatctcct gcatggtgtg
    11521 agtcatggta ctgacctgaa caccgcccgc ctgcgccgtt gtcgcggcat tttttgccag
    11581 ttccgacgcc tgtcgtgcgt tatcggcgtt ttgccctacc gtggcggtta attgctccat
    11641 actggcggcc gtttgtgcca gcgatgccgc ctgctgttcg gtacgacttg agagatcgtt
    11701 attgcctgcg acaatctccg cgatcccaat gtgcatctca tggcttccct tacgcacatc
    11761 actcaccgtc ccacgcaaag cctgctgcat ggtcttcagg ctggcaaaaa tggctaccaa
    11821 taatggccag tggttgaaca atcatcttgc gcgtccacca cagcgcactg ctgatgtaga
    11881 ttgccgcaac aataatcatg ctgataaaca ccagcgccga aatctgatag ttacgctgac
    11941 tttgcgcact ggcggcttcc agcacatggt tgatctccag ctgccaggcc tcaaagttaa
    12001 cgtcaaacgc attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa agttgattac
    12061 tttcaagcca ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa cttttctccg
    12121 tctctttttg caatgccctg acgtgctcgt tgcccgtagt catcgccata aaacttttaa
    12181 acagcgtggt ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt ttaatgtcat
    12241 ccgccggata gctaagtgcc gtcagagtgc ccgctttgtt cagcgcggta ctggcctgta
    12301 acattatcgc ccgagtttgc gctaatgccg cacgttgctg attgctctgc tcgacctgat
    12361 tcagtcgttg caaatcgtcg cgaaatgccc aaaaagacat gccgttactg ccaatctgca
    12421 agatcccgca gagaatcaaa attaaaaaca gcgtggtcga aattcgaata cgattaaaca
    12481 tcaacgctcc ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa aatgtttcct
    12541 agtttggatc ttgttcagca attcgcagtc gtggctgtgc cggtggttgc tcactggcag
    12601 gacgggatgg tgtttgcggt ttattggtga gtgggctggc tgccagacgg aacgcggaaa
    12661 ccgcttgcgt taaacgactc gcctgttctt ccagcgcagc ggcggcggca gctgattcct
    12721 gcaccagcga tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc aatgcgactt
    12781 gaacgatgcc acggctctgt tcatccgatg ccgatgcaat ctcgcccata atgtcagtca
    12841 cgcgagtgac agcattgacg atattgttca ttgtttcccc ggcgctttcg accagcaccg
    12901 aaccggtatc aacgcgtgcg acggagtctt caatgagggc tttgatctct tttgccgcct
    12961 ggacgctgcg actggcaaga ttacgcactt cacccgccac cacggcaaaa ccacggccct
    13021 gttcacccgc acgcgcggct tcaaccgcgg cattcagcgc gaggatatta gtctggaagg
    13081 caataccgtc gataacgctg ataatgtcgg caattttctt cgaactatcg gcgatctcat
    13141 gcatcgtttt cactacgcca tccaccactt tgccgccgtg ctgggcggtg tcggaggcac
    13201 tttgcgccag ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact gtcgcggtga
    13261 gctgctccat gctggcggca gtttcttcca gcgcggatgc ctgctgttca gtacgggagg
    13321 aaagatcggt gttgcccgcc gcaatttcac gggtaccggc atagatggca tctgaacctt
    13381 cgcggacatg agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg ctctgcgcca
    13441 ggtcgcccat ttcactgcgc ccgtcaatgt tttgcattcc ctgggttggc tgagcgaaat
    13501 aagctccagt attgccataa tctagataat caatcagttc agttaacgct gtgtaatagt
    13561 ttttatagtt tttatatttt tcatcaatat tacgactggt agcgaccatt tcaggtaacg
    13621 gtgccatgct tttgaatttt ttataatgcg tcgctgcctg cgccaatgtt ttcctggcgc
    13681 tatcgagcaa ttcaactttg gcgttacttt gttgattgga ggaatccatc atcatccgca
    13741 ccgctgaacg actcaggtta atgcgcgttt gcagcattaa atcccaggtt gacgtcagct
    13801 cgccctgctg ttcccgcaat tgattggaaa ccacaaagct cttctggcta tggtgaaggg
    13861 aagaaaaaaa cagactgccg gaaataagct gtaacagtgc aaataccccc aacaccatta
    13921 ccaacagcgt gactacgcgg atacggttaa tcataaggca ccttcctgaa aacaagttga
    13981 tctcgttatc ggcaagaagg ggggaaactt tattgctgat gccacccgcc gcgaaattga
    14041 aataaaaaac ccgatgcgca gatcatcggg ttcatttcaa ttgaggaaat cgggagaatt
    14101 acgccacttc tgacgccgca ctatctaaca gcgccatctc ttcgctgttc agcagttttt
    14161 cgatgttcac cagaatcaac atccggtcgc ccagtgcgcc cagtccagtg agatattctg
    14221 ttgaaagcgt cacggcaaat tccggtgccg gacgaatttg ctccgccgtc aatgaaagca
    14281 cgtctgagac gccgtcaacc acgatgccga ccacccgctg tccgagattc aggacgataa
    14341 ctaccgtgtt gtcgttatag tccacatcca cttggctgaa cttaattcgt aagtcaacaa
    14401 tcagcacaat aacgcctcgc agattcgtga cgcctttgat aaacgctggc gtgttcgcaa
    14461 tccgtgttac ctgatcgtag ccacagatct cctgctcttt caggatatca ataccgtact
    14521 cttcatcacc aagggtaaat accagaaatt cctggcctga cggctcgctg gccagctttg
    14581 ttacattcgt cataccggtc atattgttac ctttttactc attcaggcgg cggtgttcgc
    14641 catacgttgt tcgcggttta tcgcctgcaa ggcggagaca tcaacaatca gtgccacgct
    14701 gccgtcgcca agaatggtcg cagcagaaat gccggggact ttgcgatagt tactttcaag
    14761 gtttttaacc acaacctggt gctgaccaat taattgatcc accagcaagg cgtagcggcg
    14821 accgccactt tgtaagatca ccacaattcc ctgggtggct tcggttttct cgcccgcgac
    14881 gttgaacact ttccacagtt cgacgatggg cagatattcg ccccgcactt ccagcacccg
    14941 ctcgccgccg gccagtggat gcagatcggc ttcatggggt tgcagtgatt ccatgacagc
    15001 attcagcggc agaatgaaaa cttcatccgc aacgcgtacg gacatgccgt cgaggatggc
    15061 cagcgtcagc ggcagtaaaa tgcggatcgt agtgccagta ccctgcttcg actggatttc
    15121 gacatgaccg cccatctcct ggatattacg tttaacgacg tccatgccga cgccgcgccc
    15181 ggagacgtcg gtgacctgct ctgccgtgga gaagcctggt gcaaatatca gcatcgcgac
    15241 ttcgtcgtcg ctcatgtttt cgctgacagt caaaccttgc gaggccgctt ttgccagaat
    15301 tcgctcacgg tttagccccg ccccatcgtc ggtcacttca atgcaaatgt tgccgccctg
    15361 atgttcggca gacagaatta aatttccgac gctgttttta cctgcggcga gccgtttttc
    15421 tggcagttca ataccgtgat cgaggctatt gcgtaccagg tgggtcagcg ggtcgataat
    15481 gcgttctatc aggctcttgt cgagctcagt ggaactgccc accagcgtca gttctacctg
    15541 cttgccgagt tttcccgcca gatcacgcac cagccgggga tagcgactaa aaacatattc
    15601 catcggcatc atgcgaatcg acatcactga ttcctgcaaa tcacgggcgt tacgttgtaa
    15661 ctgccccatg ctggttatca aatcaccatg attaaccggg tccagttcgc tggaacgctg
    15721 ggcaagcatg gactgggtga taaccagctc gccgacgagg ttaattaatt gatcaacctt
    15781 ttctaccgct acacggatgc tggtggattt actgctgcgc gtcgtttttt cccgctccac
    15841 gcggccggtt ggtgcttgtt cggctgccag tttaagcact ggtggggtgg atatttttgg
    15901 cgagacttct actgtttcaa aggtaatctg atcggcttca atcacaaaac agagtaccgc
    15961 tgtgatgtca tcttcggcga tgtcgcccgg taatattgcc gagagcgaat ccgccccttt
    16021 caccacgtca gttaatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
    16081 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
    16141 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
    16201 gctgcaaaac cagcagatgc tgctccatat cagccaacag ttcgtccgct tcatcaaaaa
    16261 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
    16321 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
    16381 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
    16441 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
    16501 gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
    16561 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tgtaaccttt ctcaccactg
    16621 gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat accgttcagt
    16681 acaggcgcga tggcacgcag aatgtcgcgc atatagggtt cgacatcggc actgccggtt
    16741 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
    16801 tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat caactggtcg
    16861 agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag ctcttcgatg
    16921 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc gccgggaatt
    16981 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    17041 aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag ccacatcacc
    17101 agaaaaaagg ccatcatcgc agtcataaaa tcggcataag caatcttcca cgatccatgt
    17161 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgtttgattc
    17221 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    17281 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    17341 cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg cactgcatca
    17401 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc tagtggggaa ataaatccgt
    17461 aagccaataa aataccgagg aaagtcccca ccatcgcatg tgcgataagc gcccccagct
    17521 cggcggcagg acgatcggct gaacctaacg cgtgaacgac ccccattaca gccgcaacaa
    17581 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    17641 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatgt
    17701 gaccgctgat aatcaggcgc agacaatcga cgataaaatc aagcatgacg ctatccgcga
    17761 ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    17821 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    17881 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gctttcagcg
    17941 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    18001 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    18061 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    18121 tccactgttg accatgacag gacgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    18181 aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc gccacaccgt atcagttaaa
    18241 cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga ttctgggaaa
    18301 gtttacgtct ttttactgcc cgggatggcg gttgacataa gctgcaggca aagctgccaa
    18361 caggctggtg agcgtgggta ataaaattgc cgccgcagca gttgcagctg gaaagttgca
    18421 gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc agcagtggtc
    18481 cttcttctgc ttgtgggcac tgttcaaggt ataaacggta ggctttgatc accgcatcga
    18541 cgccattaca caaacctgtt ttcagtaaaa actgccatgc attacagaac atcgaagcat
    18601 gaacgttttg ttcctaggtc ataaaccagt cggttgagaa tggcagcatg cctttcggcg
    18661 gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg tccgcgactt aactgtgttt
    18721 cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc agctgaatat
    18781 cccgcgcttc ctgaacaatg cttttttcac tcatgatcag gcccttttgc agcgcttctt
    18841 caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta tgaatttgct
    18901 ggagatcgtt aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg ctgtcaaaac
    18961 ggaagtgaca aaccagttga ttggtttctg ccagcttaac catttgcgga agagtcagtg
    19021 ccgctaacgt tgtcgccatt tcttcattta tgccgagacg aaacatagcg gacgctttgt
    19081 cctgaacaat caaacgctgt gcaagtagta aatatgacaa gttgatgtca taaatgtgtt
    19141 tcagcaactc ggaggtatgc attattccca cccagaataa ccaactttat ttttatgcgg
    19201 tttcaccgca ccccgtgatg tcgccgggaa gccccggtaa aaaataatta gcattagaat
    19261 agttgcgata agctgcaata agcagaacca cctttttggt ttaatatgtc cttacaaata
    19321 gaaatgggtc tttacactta tctaagattt ttcctaaatc gacgcaactg tactcgtcac
    19381 tacacgcaca tacaacggag gggggctgcg attttcaata atgtgtgatg cagatcacac
    19441 aaaacactca attacttaac ataaatgcat aagccatact tttgttttgg gtgtatttca
    19501 atctgttaaa aagtttttcg ctacgctagc aagcaaaaat gaaactggaa taatcgaaat
    19561 gggatgttgc gcacagttaa aataactcac cgtaaataat catctgctat aaataatcac
    19621 tttcatgcaa taccagataa gctattttta aacagacact taccgcacaa caaactaatt
    19681 aaatacaaag aaaggcaaca atcaacttaa tgttaatgaa atgttttaca tatattaacc
    19741 acttgtttaa tgcggagaat cagtacacta ttttcttata attacatttg aaattatatg
    19801 atcacatgga gtgattaatt aattgtcttt actaataatc aggctaatat ttacctgttt
    19861 gaccgcgttg ggattgcgtc gtttctccat taggagtaaa gctttaatgt cacctgaagt
    19921 tcacagaata aggaacaggt tatgagctat agcaatattc ttgtcgctgt tgcggttaca
    19981 ccggaaagtc agcaactgct ggcaaaagcg gtatctatcg ccaggccagt aaagggacac
    20041 atcagtttaa ttactctcgc ttccgacccg gaaatgtaca atcaattagc cgcgccgatg
    20101 cttgaagatt tacgcagcgt gatgcaagaa gaaacgcaaa gctttcttga taagttaatt
    20161 caggatgcgg ggtatcccgt tgacaagacg tttattgcct acggtgagtt aagcgaacat
    20221 attctggaag tatgtcacaa acatcatttc gatttggtga tttgcggtaa tcacaatcac
    20281 agtttctttt cgcgagcatc ctgctcggcg aaaagagtga ttgcctcaag tgaggtcgat
    20341 gtgctgttag ttccacttac gggtgattaa ccgctcctac gcaagctttg gaaaggtagc
    20401 aactttatcg cgctgctggc tttccgcgct tcgcggaact atctgcttta ggtcgctaat
    20461 gaagcactcc tgccagtggt taatatcgtt tttcacgata acgtccagca tttctgcatg
    20521 acgggaaata cgttccgcca gcgacatagt caatgcacga tccagcgcag ctgcaacttc
    20581 gtcacgatcg taggggttaa caattaacgc cgacgttaac tcgtttgccg ctcccgcaaa
    20641 ttgcgaaaga acaagaacgc ccgggttggc tgggtcctga gcagcaacat actcttttgc
    20701 taccaggttc atcccgtcac gcagtggcgt cactaagccc acgtcagagt agcggaatat
    20761 tttcatcagt aatttacggt caaaatgctg attcaaataa taaagcggcg tccagcctaa
    20821 ttgcccgtat ttaccattaa ttcgtccagc ttcattttcg agctgatgac gaatatcctg
    20881 ataggcttgc acatcaccac gcgacgttgg tgcaatctg
//
LOCUS       DAJTMK010000064        20602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTMK010000064 DAJTMK010000000
VERSION     DAJTMK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.065"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(101..733)
                     /locus_tag="OO135_001992"
     CDS             complement(101..733)
                     /locus_tag="OO135_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5397842.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1029..1304
                     /locus_tag="OO135_001993"
     CDS             1029..1304
                     /locus_tag="OO135_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397843.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAIWFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1381..1623)
                     /locus_tag="OO135_001994"
     CDS             complement(1381..1623)
                     /locus_tag="OO135_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5397844.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            1976..2896
                     /gene="lpxP"
                     /locus_tag="OO135_001995"
     CDS             1976..2896
                     /gene="lpxP"
                     /locus_tag="OO135_001995"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5397845.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEHEKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALEKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            3252..3323
                     /gene="ypdK"
                     /locus_tag="OO135_001996"
     CDS             3252..3323
                     /gene="ypdK"
                     /locus_tag="OO135_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5397846.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3388..4626)
                     /gene="alaC"
                     /locus_tag="OO135_001997"
     CDS             complement(3388..4626)
                     /gene="alaC"
                     /locus_tag="OO135_001997"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5397847.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            5003..6700
                     /gene="ypdA"
                     /locus_tag="OO135_001998"
     CDS             5003..6700
                     /gene="ypdA"
                     /locus_tag="OO135_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR5397848.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            6715..7449
                     /gene="ypdB"
                     /locus_tag="OO135_001999"
     CDS             6715..7449
                     /gene="ypdB"
                     /locus_tag="OO135_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5397849.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            7578..8315
                     /locus_tag="OO135_002000"
     CDS             7578..8315
                     /locus_tag="OO135_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5397850.1"
                     /translation="MGSLCIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAAN
                     LPINNKPVMLLSLVFAPTWLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTH
                     LSRSPLEQNIIQPLVLSLLHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTR
                     ESVAQFFNITPNHLSKLFAQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGF
                     PDSDYFCRVFRRQFGLTPGEYSARFQG"
     gene            complement(8318..10813)
                     /gene="ptsP"
                     /locus_tag="OO135_002001"
     CDS             complement(8318..10813)
                     /gene="ptsP"
                     /locus_tag="OO135_002001"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5397851.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRCLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSGIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGCTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEALG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQFRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(10838..11875)
                     /gene="ypdE"
                     /locus_tag="OO135_002002"
                     /pseudo
     CDS             complement(10838..11875)
                     /gene="ypdE"
                     /locus_tag="OO135_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11875..12960)
                     /gene="ypdF"
                     /locus_tag="OO135_002003"
     CDS             complement(11875..12960)
                     /gene="ypdF"
                     /locus_tag="OO135_002003"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5397852.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(12975..14222)
                     /locus_tag="OO135_002004"
     CDS             complement(12975..14222)
                     /locus_tag="OO135_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5397853.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(14244..14570)
                     /locus_tag="OO135_002005"
     CDS             complement(14244..14570)
                     /locus_tag="OO135_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCR5397854.1"
                     /translation="MSKKLIALCACPTGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(14789..15754)
                     /gene="glk"
                     /locus_tag="OO135_002006"
     CDS             complement(14789..15754)
                     /gene="glk"
                     /locus_tag="OO135_002006"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5397855.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            15958..17214
                     /locus_tag="OO135_002007"
     CDS             15958..17214
                     /locus_tag="OO135_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5397856.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAGGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            17329..17655
                     /locus_tag="OO135_002008"
     CDS             17329..17655
                     /locus_tag="OO135_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5397857.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(17796..19034)
                     /locus_tag="OO135_002009"
     CDS             complement(17796..19034)
                     /locus_tag="OO135_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5397858.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            19370..20572
                     /gene="nupC"
                     /locus_tag="OO135_002010"
     CDS             19370..20572
                     /gene="nupC"
                     /locus_tag="OO135_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5397859.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVV"
BASE COUNT         5020 a         5441 c         5067 g         5074 t
ORIGIN      
        1 gccaaataat tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcggctgga
       61 gggagttaaa tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt
      121 cgctgtcgac gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt
      181 gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca
      241 tcagatattg gttttccatt acgccaacac cggccaggcg aagttcttcc attgcgccct
      301 ttttatcccc tttcgccatt ttttcattgg caatttttat cgcggcttct ttttcgggag
      361 ttgctacata actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat
      421 tgagattggt ctttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca
      481 gcaaagcgga agcttcattg gtcagttctt ttgccttttc tggatcgcca tgaaacagag
      541 ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg
      601 ctgctgccgt tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc gctaccggtg
      661 cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa
      721 ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc catcatattg
      781 ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacagt
      841 tgtagttatt atcgcaagaa gtattttagt cttacaattg taggaatctg cttcctgtcg
      901 cagagagcta ttcttatgta ccacaaagag atgtcattta ttatatcgat gtaaatattg
      961 attaattata tgtaaataca gccataacat cgtaggttct cagtgaacca ttttcaggat
     1021 aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc tctattatgg
     1081 catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc tataaagaga
     1141 ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca tcacaggcga
     1201 tttggtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa atcacgccta
     1261 cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg tcacatggca
     1321 ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca
     1381 ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa
     1441 cataaatatc cccgtcacta gttcattacc gccagttaac cacatggtga agtaataaat
     1501 gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca cgttcagtac
     1561 ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat
     1621 catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga
     1681 caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcatg ttaaatgcaa
     1741 gactgttgtg tacggaaaaa tatttacttt gcacgattaa taatcagtag ctgaaagcag
     1801 tcagcgagtg ataaagtaca gataccgcat tcatgctcct cgtggttatg tcctgactag
     1861 tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat tgagtgtacc
     1921 gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt
     1981 tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca catggtttgg
     2041 gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac
     2101 gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa
     2161 cctggaactt tgtttcccgc agcattctgc ggaagaacac gagaagatga ttgccgaaaa
     2221 ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct ggcccgacag
     2281 tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg cacaaatgca
     2341 aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt
     2401 gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc agctgatgga
     2461 atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca gaaataatct
     2521 gcgcggcatt gtcggtgcac tggagaaagg tgaagcggta tggtttgctc ccgatcagga
     2581 ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac
     2641 caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg taacgatggt
     2701 aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg aaggctaccc
     2761 gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag agatcatgcg
     2821 cgcaccggag cagtacctct ggatccactg tcgctttaaa acgcgcccgg tgggagaatc
     2881 atcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg ccataataac
     2941 ctcatagttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat ctaattatta
     3001 ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa agtaaataag
     3061 aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctt gagcagttta
     3121 gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt cgctttgccc
     3181 ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagc aattacgcgg
     3241 agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc gtttgggccg
     3301 gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc
     3361 tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat gtttgctgct
     3421 ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg cctgacgaat
     3481 acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt cgccaaagcc
     3541 aatccccggc gagacacaga ccttcgcttc gttaagcaat ttcttggcaa ataccagcga
     3601 gcccatggca gcatacggtt ccggtatttt cgcccagaca tacatcgaag ccttcggcat
     3661 ttcaactatc cagcccgctt catgcagccc tttaaccaat acatcacggc ggcgtttgta
     3721 ctgttcggca atgtcgcgca cgcactgttg atcgccctcc agcgccgcaa tcgccgccac
     3781 ctgcaacggc gtaaaggtgc cgtaatcgtg atagctttta atacgagcca gggcgctgac
     3841 cagcgttttg ttgccaacca taaagccgat acgccagccc gccatgttgt agcttttcga
     3901 cagcgtaaag aactcgactg ccacatcgcg tgcacccggt acctgcatga ttgacggcgc
     3961 tttccagcca tcgtagacga tatcggcata ggccaggtcg tggaccacca gcacatcgta
     4021 gcgtttcgcc agcgccacaa ccttttcgaa gaactccagc tccacgcatt gcgcggttgg
     4081 attcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac gaatggcgcg
     4141 ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta cctgcgcccc
     4201 ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca gcaccgtgtc
     4261 accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac caatagtgac
     4321 gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc agcgggaaat
     4381 ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt ccgggcgctg
     4441 ggccacagta cataattttt cgacgatatg cggtggagtc gcaccgtccg ggttacccat
     4501 gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt cagcggtgat
     4561 gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc
     4621 agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg ctgccacaat
     4681 ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt agaacatttt
     4741 tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc tacgctaaca
     4801 agaaaattac caccaatttt attaacatta taatttcaat aaatttacca gccagaagtg
     4861 cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt ttaaatttat
     4921 ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat catagaggtt
     4981 taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg cggtcttcga
     5041 tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc tgtttcgcga
     5101 gctgttgcac aagtcggcac actccccaaa agagttgctt gctgttaccg ccattttctc
     5161 gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc tggtgaacgt
     5221 acgtattatc gcggtgatgt ccggcgggat tctgtttggc ccgtgggtag gcatcattac
     5281 cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga cggcgatccc
     5341 ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc tgaaaatccc
     5401 caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga cgttgaccat
     5461 gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg tctctaaaat
     5521 cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc tggtgcaaag
     5581 cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc tggatatcgc
     5641 caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca aggtctgcga
     5701 aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg atcatgtgct
     5761 ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca ttagcccgac
     5821 tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg aagcccaccg
     5881 cacaccagag attcattcca tgctggtgat cccattgtgg gagaaaggcg ttgtgaccgg
     5941 aacgctgaaa atttactact gccacgcgca tcagataacc tcgtcattac aggaaatggc
     6001 ggttggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg agcagctacg
     6061 tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc cccattttct
     6121 gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata ccgctcgcca
     6181 gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag acgatgagca
     6241 aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg agcaggcccg
     6301 ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt gctgcattcc
     6361 cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc agccttgcaa
     6421 aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac gcattgcggt
     6481 gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag cgaatgaaat
     6541 gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat tgtatggcga
     6601 ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca ttcctaacca
     6661 acgcacccca gtcgcctcac aggctacgtt attgctttga gccgaagtga tattgtgaaa
     6721 gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct aattaaagag
     6781 cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt gaagtttttg
     6841 cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct ggatggcgtg
     6901 ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt catcaccgcg
     6961 tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat tctcaaaccg
     7021 tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg gcaacaacag
     7081 cagaccagca gtacgcctgc agcgacggta acgcgtgaga atgacaccat taatctggtg
     7141 aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga agcgcacgag
     7201 aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa cattaccgaa
     7261 ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt ttgtgtcaat
     7321 ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct gcgactgaaa
     7381 gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg ccagttaatg
     7441 catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca acaatttttc
     7501 gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt ctggtttgcc
     7561 agccagcctc gttaccggtg ggcagtttat gtattgattt tccccgtctg gatatcgtgc
     7621 tgcgcggcga atacggcaac ctgctggaag caaagcagca gcgaatggtg gaaggagaaa
     7681 tgctgtttat tccggcacgc gcggctaatt taccgatcaa caacaaaccg gtgatgctgt
     7741 taagcctggt gttcgcaccg acctggcttg ggttatcgtt ttacgatagc cgcaccacgt
     7801 cgttgttgca tcctgctcgc cagatccagc ttcccagcct gcaacgcggt gaaggtgaag
     7861 cgatgcttac cgccctcact catcttagcc gttcgccgct ggagcagaat atcattcagc
     7921 cactggtgtt aagtttgctg catctttgcc gcaacgtggt gaatatgccg ccgggtaatt
     7981 cgcaaccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag gataattata
     8041 cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc aatcatctgt
     8101 caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt tgggtgcgaa
     8161 tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc aattcatgaa gtggcacagc
     8221 gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag tttggtctga
     8281 cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat tctgtttcca
     8341 gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg aagtttttat
     8401 tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct tcgttagcac
     8461 ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag tcaatcggtt
     8521 tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg gtgtgcggga
     8581 tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc ccgctgccag acatcttctt
     8641 ccagctcaaa cggatgttca gtgcgcccgt taacgccgag gttgccgcac aggaactgga
     8701 tcgcctgctc tttattgcta aaatcctgat caacaaagat attctccagc gccagcagtg
     8761 ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc tgcgcactgc
     8821 ggcattcaca tgcctgacgc gccagttccc gacacgcctc gctatccagt tgacgaaact
     8881 ggcttttcac cgccggaata cgcgggctac tcatactcag ctcatccagg cccagcccaa
     8941 gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct acccatttgc
     9001 cccgctgatg cgcagtggta actatttgct gcaacatgcg caggaacgat ggcgtaatcg
     9061 ggttatatag cggcgatacg cgcgggttat tacgatcgac cgcatacaga tactgggtca
     9121 tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtggtcg atgatgtagc
     9181 acaccgacgg cacttccacc atgatcccaa gcgtaatcgt ctctgcatga cgcaggccat
     9241 cgcgcttaag ctcaacgatc gctttttgga tctcgccctt cacccataag atctgatcga
     9301 gactgtggac catcgggatc atcaactggg cgttgccaaa actggcggcg cgcaaaatcg
     9361 cccgtagttg agtgcggaac aggccagcaa attccgggta aatacgcacc gcgcgatagc
     9421 cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta tcgccaccga
     9481 tgtccattgt gcggaagata atcggcttgt cgcccgctgc cagtagcacc tgctggtagg
     9541 cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc catatagagc atttcagtac
     9601 ggaacagtcc aacgccttcc gcgccgttgg caaacgcgcc tagcgcttcc agagcggtgc
     9661 cgatattcgc cgcaatatca atacgcttgt tatcacggga ataggccagc tgcgcggcgg
     9721 cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg tgcgacctga taataaccgc
     9781 tcaccgcgtc attcgggtta atcgccagca cgccgcactg ggcgtcaagc accgccggtt
     9841 gcccggcata acgggcaatc gcatccagcg gcaggccgct cagtaccggg atcgccgagg
     9901 cgcgggccag aatcagggta tgcgaggtgc acccagtttt ctccagaatc atgcctgcaa
     9961 gattttttaa atcgaggctc aaaaactggc ttggggttaa atcctcggct accagaatag
    10021 tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc cggccaggtg atatgcagca
    10081 actgttcgct aatatcgcga atgccgctaa cacgttcgcg cagataatca ctggcagaag
    10141 cagaaagttt ggcgcaaacc tgctccatat tgctgatgat cgccgccccc agcccttgat
    10201 gctgttctgt catcagacaa cggatattgc ccgcgaattc atcatcctga atcagcgaca
    10261 aatgggcgct aaggatagtt ttgctttcgc cgtcacgctc acgcaattgc tggttcagtt
    10321 gctcggcaag cgttgcaagg ctgtgctcca gccgggtgga atcttgcgca ctggcgggga
    10381 ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc acgccgacgc
    10441 tgcttgccag cacattgccg tacagtaaat ccgggttcag gcggcttaat gaacgcggca
    10501 gcggatgcgc cgtcagttct gcctgcgttg gctggatgct gtcgctgtcg ataaagcgca
    10561 cctggatgta ctcttccaac acgcgctgcg cctgctcttc atcgctgccg ctaatgttca
    10621 ggctgcaact gtcattaaat aaagtaccgg tgccaatcag cgccagcgag cttttcgcat
    10681 cggcctttgc gttctggcga tggttgataa aggtgatttc gctttgccac tggctgcact
    10741 gttctttaag ttcccacgcc ggacgagcat gaagaccgtt tggcaaagga cagagaaatt
    10801 gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc gtcagttgaa
    10861 caaccgtctc acgcgtaaga cgtttaataa gggcagataa aagctgctgc atctgcaaaa
    10921 tgtcacggca atcggcaatc gatgcggcgc aatgtccgtg gcgggttgct ggccccatca
    10981 ccactgtagg cacgccggtg ccggttaagt gcaccgctcc gccgtccgtg ccgccgttgc
    11041 tgaacatatc tgcctgtaac ggcacgccaa tttctgccgc cacggtttag atccacgcgg
    11101 taagttttgg cggcgcaatc agcgacttgt cgcttaacac cagcatcggc ccgttacaaa
    11161 tctgacgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg gtatcaagca
    11221 caatggcgac atccggcgac accgcgcggg tggcagtttg cccgccgcgt acccccacct
    11281 cttcgctgga actggccacc agccacactt ccgcaggcag ttcagcgctg tgtagttcgc
    11341 gcagtaacgt caccagcagg tagcaaccga ggcggtcatc aaaggctttc cccatcaccc
    11401 gctggtgagg gagaacctga aaagttgtat caaaggtgac gcgatcgcct ggacgaattc
    11461 ccgcctgcat cacttcgtca tacgagcgcg taccaatgtc cacgcgcatg gcgctgacgt
    11521 cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca cgggtggtga
    11581 tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc agcacatcaa
    11641 ttgccccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata tgggcgcaga
    11701 tcatcacctt cggccctgtc gattcattga ggcggatcag caccgatccc agaccatcaa
    11761 atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc acttcctgtt
    11821 ccgaggaggc gatcgcatct gcctcgctca acgcttttaa tagcgataaa tccattatgc
    11881 ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc cttgcggggt
    11941 aaccagcaca acatcttcga tgcgcacgcc cccttgccct ggcaaataaa tccccggctc
    12001 cacggtcagc aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa aacgcggatc
    12061 ttcatggact tcaatgccga tagcgtgacc agtgttatga ccgaaatagt cgccaaatcc
    12121 agcctcggta atgacccggc gcgcggcgtc gtcaatctgc tggcagcgca cgccggggcg
    12181 aattgcagag attgccgcga gctgtgcctg caggacaatc tgatagacgt caaacagcgg
    12241 gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca tatcagagca
    12301 gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg caacaatctt
    12361 gtcgctggct ttgccgtgcg gcagcacccc gcgccagccg ctggcgacaa tggtgtcaaa
    12421 agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg ctatctcgcg
    12481 ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat cggcaatccc
    12541 acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca gcacatccgg
    12601 cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg tttcccaact
    12661 cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga tttgattgac
    12721 gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt gcgcacgggc
    12781 ttccacctca acgaaatagc gcgaatctac cagaatgtgc gcactttcac ggctaataac
    12841 cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc gtgaggagag
    12901 aagcactgca tccagttgtt gcgccttaag ccagtcgcgc agcgaagcga gtaatgtcat
    12961 ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa catcatcaga
    13021 cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc cgccatatag
    13081 acgccaaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa ccattgcact
    13141 gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata cgacggaatg
    13201 gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc cataaaggca
    13261 aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcg tttgccggtt
    13321 aaacgacggt caataatggt cgccagcccc agaccaatcg gaggaataac gatagcgata
    13381 gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg
    13441 gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac
    13501 atcagcgcgc ccttctcacc tgcggcagtc agcacggtac ggataccgcc gttgatccag
    13561 ccgccaaagg gggtaatgac gtagtacatc gccagcatga caaaaatggc ggaaagaatc
    13621 ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat tttctggttc
    13681 atccacttca caaggtaacc cgcgacgata gaaataatca gcgcaccaat gaaggtggac
    13741 ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag cagttgcgtc
    13801 ggctgggtag acatcaagcc gccgataaaa ccagccggga acgccagttt gccaccgata
    13861 gagttcgcca caaaagcagc aaacatcgga atggcaaaac caaacaacac gccgccgaag
    13921 gactgcgaca gccaggcaaa tttcagtaag gagaggtcga aaccggagaa tttcccgcta
    13981 ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata agcaatcagc
    14041 tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg tgaaatccct
    14101 gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg cggattctta
    14161 actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt tttaatggcc
    14221 ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc ttcgatttct
    14281 ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt
    14341 gattcgaaac gctcattatc ttccggggta actgccacgg agtggatgat gatggttgct
    14401 tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg ggtttcaatt
    14461 ttcacttcat aaccggcttc taccgccgct tcttccaacg cctgagcggc cataaaggtg
    14521 tgagccaggc ccgttgggca ggcacataag gcaatcagtt tcttactcat cgttaattcc
    14581 tcattcagtg atagttgacg agtgatatta tgaacgtgca aagcaaatcg ccgttaccag
    14641 acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc
    14701 cttgtgaggg gacacaaaga aaaattgaat taactgtatg atttaaaaga ttatcgggag
    14761 agttacctcc cgatataaca ggaaggattt acagaatgtg ccctaaggtc tggcgtaaat
    14821 gtgcaccgga accgagaaga cccggattgt catggacgat gagatacacc ggaatatcat
    14881 ggacatattc tttaaagcgc cctttatctt caaatgcagc acggaaacca gaggctttga
    14941 agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca aatgtcccga
    15001 gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc gacaacgcgc
    15061 ggcggcaatc ggtacagctg tcagccagcg cgcgttcggt aatatctttt ggcttgagat
    15121 tttctggcag gcggttgtca gctttcacaa ttgcgcgata caaattcacc agcccagggc
    15181 cagaaagcac gcgctccgcc gaaacatgac caatttccgc acgcaatatt tcgaggataa
    15241 tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta
    15301 cccaacgctt atcgacatgg accagatgcg caaccccaag ccctgttccg gcaccgtaaa
    15361 ccgcaatagg tttaccttct accggttctg cgccaccaaa ctgaatcaga tgctcttttt
    15421 tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc agatggctaa
    15481 aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggtta gtcattgcca
    15541 cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc tcgaccttat
    15601 gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc cctgaatagg
    15661 tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt gcgttggtgc
    15721 cgcccacatc accgactaat gcatacttta tcattcttca actgctccgc taaagtcaaa
    15781 ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg aaaggcaggc
    15841 tccctgtaaa tatcgatctg ggtcacacat ttactttatc gtttcagcac caattgcagc
    15901 gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga atagaacatg
    15961 ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc aattgggatt
    16021 gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca gaatttgctc
    16081 tggcaacgac tgccgggaac tctggggata gcccaggatt cacctctctg gatcatcggt
    16141 gtattaacgc taacgggcat tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc
    16201 ggaccagacc ccgcctgtga accgctgatc ggcgcaccag ttccgccctc tgcgctacca
    16261 ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg gccggaacat
    16321 ccgatcatga ccgtcaatat cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc
    16381 gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc gctgtttggc
    16441 acacctgttg cagcggcgtt gatattttcg caaaccttaa atggcagtag tgaagttccg
    16501 ctatgggatc gtctctttgc gccgttaatg gcggcagcag gtggtgcact aactaccgga
    16561 ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat ggaaatgacc
    16621 gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcagggat ggtcgccgta
    16681 tggtgcttac cacggttgca cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg
    16741 ggtattggcg gatttattct cggtattctg ggggttattg gtggaccagt ttcgctgttt
    16801 aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac cagcgattac
    16861 tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag tggctttcgc
    16921 ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat gctgcatgag
    16981 cacgtacccg ccgtaccagc ggcaataacc gtttcttgcg ctattctcgg catcgtgctg
    17041 gtggtaacac gcgatggctg gttaagtctt tttatggcgg cggtcgttgt acccaatacc
    17101 acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt agcaggtaag
    17161 ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataacgt ttagcaaaaa
    17221 atgctctcct ttatgattta agagttatgg cgatgatacg gggctttacc tccccgtaat
    17281 attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat gttcaggtca
    17341 ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaacgc aggtgaaatc
    17401 accctactgc catcaatcaa attacaaatt ggcgatcgcg atcattacgg taattactgg
    17461 gacggtggtc actggcgcga ccgtgactac tggcatcgca attatgagtg gcgcaaaaac
    17521 cgttggtggc gtcatgataa tggctatcat cgtggctggg ataagcgtaa agcgtatgag
    17581 cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat
    17641 ggacaccgcc attaaacaca ttcgccaatg gagcacaatg cctgatgcga cgctagcgcg
    17701 tcttatcagg cctacaaacc ggatctttaa cgcgtagggc ggataaggcg tttacgccgc
    17761 atccgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc cgtccccacc
    17821 aacaaccaga tattcagcgc cacgaccagc actacaatca cccagcctgt ctgttttacg
    17881 cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga
    17941 accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag aatccgtgtc
    18001 ggatctaatc ccatcagaat gacaataaat gacggcagca tggtgactgt acgacgcacc
    18061 cacagcggga tatggaagcg aatgaacccc tgcatcacca cctgccctgc cagtgtcccc
    18121 actaccgttg aggacagtcc ggcagcaacc agacttaacc caaagaccgt tgccgcagca
    18181 tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcggcaac accagtatga
    18241 ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac aaaaccggca
    18301 atagtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg acgcgaaccg
    18361 ccatgtaaat gctgagtgag cgaggagtgc aaataaatca catgcggcat aatcgtcgcc
    18421 cctaacacgc ctgctgccag gaagaccgct tccgaagtag gtaaactcgg gatcaccatt
    18481 cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat gtaagccgcg
    18541 gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc gcgacgttgc
    18601 agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca
    18661 ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc ggttgccatc
    18721 gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata gtgatcgcga
    18781 atctgctccg ccagattttt accggtggca atccctagtt tggcagagag gatctgaatc
    18841 agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc gaagctggca
    18901 cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac
    18961 gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct actctcaacg
    19021 cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca tgctatgata
    19081 aacgagaatg attatcaaat tcatttaaat ggattgtggt gatttctgtg atagaccagg
    19141 attatgacta cgaaaagatt gatgacttga atgttatggt tatgtttaat gttagcacat
    19201 ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacaga tcactatttt
    19261 tgaagcctgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt
    19321 tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata tggaccgagt
    19381 ccttcatttt gtactggcac ttgccgttgt tgcgattctc gcactgctgg taagcagcga
    19441 ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag tgttactggc
    19501 gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt
    19561 cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta gcatgaatga
    19621 tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta tctctgcact
    19681 gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatc atccgcgcaa ttggtttcct
    19741 gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca gctccctgat
    19801 tctgggtcag tctgaaaact ttattgccta taaagatatc ctcggcaaaa tctcccgtaa
    19861 tcgtatgtac accatggctg ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc
    19921 atacatgacc atgctggaac cgaaatacgt cgttgctgcg ctggtattga acatgttcag
    19981 cacctttatc gtgctgtcgc tgatcaaccc ttaccgtgtt gatgccagtg aagaaaacat
    20041 tcagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg aatacattct
    20101 ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg atcggcttta tcgccctgat
    20161 cgctgcgctg aacgcactgt ttgccaccgt gactggctgg tttggctaca gcatctcctt
    20221 ccagggcatt ctgggctaca tcttctatcc gattgcatgg gtgatgggtg ttccttccag
    20281 tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg agttcgttgc
    20341 gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag gcatcatctc
    20401 tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcag gtgcagttaa
    20461 aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg
    20521 ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtggtgt aagaccatac
    20581 ataaaaaagc cggggataat tc
//
LOCUS       DAJTMK010000065        20373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTMK010000065 DAJTMK010000000
VERSION     DAJTMK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20373
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.066"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..670
                     /locus_tag="OO135_002011"
     CDS             <1..670
                     /locus_tag="OO135_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR5397860.1"
                     /translation="TYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQDL
                     QLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRELM
                     LLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEINGSE
                     KELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQPKLRGNVQTAQR
                     EVAP"
     gene            667..1755
                     /locus_tag="OO135_002012"
     CDS             667..1755
                     /locus_tag="OO135_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5397861.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLYSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTSTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKSVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(1843..3141)
                     /gene="exuT"
                     /locus_tag="OO135_002013"
     CDS             complement(1843..3141)
                     /gene="exuT"
                     /locus_tag="OO135_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5397862.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATTNGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3624..5036
                     /gene="uxaC"
                     /locus_tag="OO135_002014"
     CDS             3624..5036
                     /gene="uxaC"
                     /locus_tag="OO135_002014"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5397863.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5051..6527
                     /gene="uxaA"
                     /locus_tag="OO135_002015"
                     /pseudo
     CDS             5051..6527
                     /gene="uxaA"
                     /locus_tag="OO135_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6610..7161
                     /locus_tag="OO135_002016"
     CDS             6610..7161
                     /locus_tag="OO135_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5397864.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7166..8410)
                     /gene="sstT"
                     /locus_tag="OO135_002017"
     CDS             complement(7166..8410)
                     /gene="sstT"
                     /locus_tag="OO135_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5397865.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9025..9990)
                     /gene="alx"
                     /locus_tag="OO135_002018"
     CDS             complement(9025..9990)
                     /gene="alx"
                     /locus_tag="OO135_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5397866.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVAEPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(10274..11260)
                     /locus_tag="OO135_002019"
     CDS             complement(10274..11260)
                     /locus_tag="OO135_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5397867.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11339..12022)
                     /locus_tag="OO135_002020"
     CDS             complement(11339..12022)
                     /locus_tag="OO135_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5397868.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNAGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ESGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12099..12602)
                     /locus_tag="OO135_002021"
     CDS             complement(12099..12602)
                     /locus_tag="OO135_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5397869.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVLGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12687..13823
                     /gene="rlmG"
                     /locus_tag="OO135_002022"
     CDS             12687..13823
                     /gene="rlmG"
                     /locus_tag="OO135_002022"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5397870.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQEPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKAHDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14108..14422
                     /gene="higB"
                     /locus_tag="OO135_002023"
     CDS             14108..14422
                     /gene="higB"
                     /locus_tag="OO135_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5397871.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14419..14835
                     /gene="higA"
                     /locus_tag="OO135_002024"
     CDS             14419..14835
                     /gene="higA"
                     /locus_tag="OO135_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5397872.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(14880..16898)
                     /gene="fadH"
                     /locus_tag="OO135_002025"
     CDS             complement(14880..16898)
                     /gene="fadH"
                     /locus_tag="OO135_002025"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5397873.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASRIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(17124..19475)
                     /gene="ygjK"
                     /locus_tag="OO135_002026"
                     /pseudo
     CDS             complement(17124..19475)
                     /gene="ygjK"
                     /locus_tag="OO135_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19492..20319)
                     /locus_tag="OO135_002027"
     CDS             complement(19492..20319)
                     /locus_tag="OO135_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5397874.1"
                     /translation="MKYGSWFGLIYGEGWTVQADHDGNAWVPDHSWGGFEGGINRFYG
                     GYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGYVIPNTRDIMTALKVQNLSGNFRY
                     SVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMMNGYIQYDLAEGITWMNGLEITDG
                     TGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYA
                     KHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         4975 a         5346 c         5338 g         4714 t
ORIGIN      
        1 cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag ccagtggtac
       61 agacgatttg ctgggtgcgg tattggtgga tgtgaaaggc ttctctgaac aggatgaaga
      121 gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc agcttggtca
      181 aagcgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg aggttaacga
      241 cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag ggaatcgtga
      301 attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct ttaactacaa
      361 ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg tggggctgaa
      421 cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta acggcagcga
      481 aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga gtgttgaaaa
      541 gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga cttattcaca
      601 attaccggag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc aaagggaggt
      661 tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac gctcactggc
      721 gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc tggtagagcg
      781 gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat gtggtatgaa
      841 tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc gacagtacaa
      901 atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa aggatttaaa
      961 acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa agggcttcag
     1021 acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca ttattatata
     1081 tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa tttgccgatt
     1141 ggtggcgtct ggaaggggaa agttaaatta tattcgaaca gcccggccca ggactatttc
     1201 gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt cccggagttc
     1261 gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt taacggcagc
     1321 aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt taacggtaat
     1381 gccatcagtt atgaaatcat gctcaaagat gaagggagac cagctgcagg gcgcagagac
     1441 ggtgacttct ctatctatcg tcagggagga acctctaccg acgagggaga acgcattgat
     1501 taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa
     1561 aatatggtct ggaacagcat taacctgaaa agtgtgcgtc cggtggtact gcccggtatt
     1621 cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa attcagcgtg
     1681 atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac gccttccttg
     1741 ccaaccatca attaaccctg caaagcggac aaacgctttt ttctatataa acgacgaagc
     1801 cgcctgataa ggcggcttcg tcgttataac ttacgttaaa cgttaatgtt gcggtgcggg
     1861 atcgttatgt gattcctgcg ctacctcaat cgccggtttg ttctgcaaca cggtccagat
     1921 aaccagcgca cctaacaggt cgaacactgc cagtactgcg aacagcgggc tgaagccgat
     1981 ggtgtcagcc agtgcaccga caaccagcga aaacagcgta cttgccagcc atgcggacat
     2041 cccggttaaa ccgttcgtcg tcgccacttc gttacgaccg aacacgtcag aagagagcgt
     2101 aatcagcgcg ccagacagtg cctggtgagc aaaaccaccg atacacagca gcataattgc
     2161 gacatacggg ttggtgaaca ggccgatcat acccgggcca atcatcagca ctgcacccag
     2221 cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg
     2281 cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa
     2341 catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc
     2401 gttaaaagta ccccaggctg gttctgccag gaaacgcggc agcgcgatac cccagaactg
     2461 acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc
     2521 ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg
     2581 atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat
     2641 aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg gcgcaatcat
     2701 cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct ctttcgccgg
     2761 gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac
     2821 tgcaccacga gcaatcgcca ggccacccct gctaccagcc agcgcggttg caccacagaa
     2881 tacggcccac agtacagcaa acattgcata accgattttc gtacccagca catccagtac
     2941 atagcccgct accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga
     3001 atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt
     3061 gttacgcgtc aggtaaccaa gcacggtgcc aagcgtcacc agtgcgatca tataccaacg
     3121 taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg
     3181 gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaccg
     3241 gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta ccggttaacc
     3301 cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta tgatttgcga
     3361 cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcata aatgtgcgaa
     3421 tatttgtagg gatattacct gacgacagcg aggccagtac tggcgcggcc tgcagcgaga
     3481 tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca cgaaaatatc
     3541 ttcgaactct ggaaattggt gtgataactt tgtcagcatc gcaccataag caagctagct
     3601 cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc ctgttagata
     3661 ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt ttcgattacc
     3721 attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac ctgtatgaca
     3781 tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt gtggccgagc
     3841 gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg gcgactgttc
     3901 cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt cgtccgtttg
     3961 gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac gaatgtaacg
     4021 aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg aacgtgaaaa
     4081 tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag atcgccaaag
     4141 acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc ttcaacatcg
     4201 aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat accgacattc
     4261 gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc gccgctcacg
     4321 gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg aacgaagcgg
     4381 aactggacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag cacgaagtgg
     4441 cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt cgcggctggg
     4501 tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc aaactgctgg
     4561 ggccggatgt aggctttgac tccatcaacg accgtccgat ggcagaagag ttgtctaagc
     4621 tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctgtac tgcctgaacc
     4681 cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa ggtatgccgg
     4741 gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt atggaacgtc
     4801 agatgaccca actggcgcag ctcggtttgc tgagccgctt tgtcggtatg ctgactgaca
     4861 gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg tgccagatga
     4921 tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg ggcgagatgg
     4981 tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg aactaaggtc
     5041 tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg tcgctttagc
     5101 ggatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc tgcgccagga
     5161 tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca atgtcatcaa
     5221 atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac acgtccacgc
     5281 ccacaatacg cgcacgaatc tgagcgatct ggatcagtcc tgattttcag gatctgcctg
     5341 cgcaagcggc agatcgtgaa gtgcagatct atcgtcgcgc taacggcgat gtcggggggc
     5401 gtaacgagct gtggatcctg ccaaccgtgg gctgtgtcaa cggcatcgcg cggcagatcc
     5461 agaaccgttt tctgaaagag accaacaacg ctgaaggtac cgacggcgtg ttcctcttca
     5521 gccacaccta cggctgctca cagctgggcg acgatcacat taatacccgc accatgctgc
     5581 aaaacatggt gcgccacccg aacgcaggcg cagtgctggt gattggtctg ggctgtgaaa
     5641 acaaccaggt tgccgcattc cgtgaaacgc tgggcgatat cgatcctgaa cgcgttcatt
     5701 tcatgatctg ccaacagcag gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc
     5761 tgtataacgt gatgcgcaac gataaacgcg agccaggcaa actcagcgaa ctgaagtttg
     5821 gtctggagtg cggtggttct gacggtcttt ctggtattac tgctaacccg atgctggggc
     5881 gtttctctga ctacgtgatt gctaacggcg gtactaccgt actgaccgaa gtgccggaga
     5941 tgtttggcgc agagcagttg ctgatggacc attgccgcga cgaagcaacg tttgaaaaac
     6001 tggtcaccat ggtcaacgac tttaaacagt actttattgc ccatgatcag ccgatctacg
     6061 aaaacccatc accggggaac aaagcgggcg gtatcaccac gctggaagac aaatcacttg
     6121 gctgtaccca gaaagcgggt tccagtgtcg tgggtgacgt gctgcgttac ggcgagcgtc
     6181 tgaaaacgcc agggctgaac ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg
     6241 ccctggcggg tgcgggctgc catatggtgc tgttcagtac tggtcgcggc acgccatacg
     6301 gtggatttgt gccgacggtg aaaatcgcca ccaacagtga actggcggcg aagaaaaaac
     6361 actggctcga ctttgacgcg ggtcagctga tccacggtaa agcgatgccg cagttgctgg
     6421 aagaatttat cgacaccatc gttgagtttg ccaacggtaa acaaacctgc aacgagcgta
     6481 acgacttccg cgaactggcg atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt
     6541 tgttaataaa aaacggcgtt tcataatgtg aagcgccgtt tgcttttcca tacctgataa
     6601 ggatttatca tgaccgcgta ttggctggcc cagggcgtgg gtttcatcgc ctttctgatt
     6661 ggtatcacaa catttttcaa tcgtgacgaa cgtcgcttca aaaagcagct ttcggtctat
     6721 agcgccgtta ttggcgtaca tttttttctt ctgggcacct atcccgctgg tgccagtgcc
     6781 atccttaatg ccattcgtac attgattacc ttacgcacgc gcagcttatg ggtaatggcg
     6841 atttttattg tgctgactgg cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta
     6901 ttgccggtta tcggtacgat tgtcagtacc agggcgctgt tccgctgtaa agggctcacc
     6961 atgcgctgcg tgatgtggtt ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg
     7021 tcgataggcg gcacaatgat tgagggtagt tttctgctta tgaatggctt gaatatcatt
     7081 cgtttctggc ggatgcagaa aaggggaatt gatccgttta aagtagagaa aacccctccc
     7141 gccgtagacg aaaggggtta aacaattaat tacgcagggc gctatttgcc agacgatcgt
     7201 cttccgcctg gcaagctgcc gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt
     7261 cgcaagagtc ctgcaatacg ccgatgataa agccgacggc aaccacctgc atggcgatat
     7321 cgttcgaaat accgaacata ttacaggcca gcgggatcag cagcagagaa ccccccgcca
     7381 cgccggatgc gccacaggca cacagagaag ccaccacgct caacagcagc gccgtgggca
     7441 gatcgaccgg gatacccagc gtattaaccg cagccagcgt caacacggta atggtgattg
     7501 ctgcgcccgc catattgatg gtggcaccca gcggaataga aacggaatag gtatcgcgat
     7561 ccagattcag cttttcacac agtgccatat tcaccggaat gttcgccgca gagctgcggg
     7621 tgaagaaggc atacacgccg ctttcgcgca ggcacagcag caccagcggg aacgggttac
     7681 gacgaatttt ccaccacacc agcaatgggt taaccaccag cgccaccagt aacatacagc
     7741 caaccagcac gaccagcagt tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca
     7801 gggtagaaga aaccagacca aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca
     7861 taaaggtgac ggcattcgac agatcgttga ccaggttttt ggtggtctcg ttaccgtgac
     7921 gcagtgcgaa accgaggcca attgcccaca ccaggatccc gatgtagtta cctttcagca
     7981 gcgcgtcgat ggggttggaa accatgctca ttaccagccc gcgcatcact tctacaatgc
     8041 ctgacggtgg cgaaatatca cccgcgctac tggataagtg cagggtagaa gggaaggcga
     8101 agctgaaaac gactgcggcc agagcagcag agaaagtacc cagcagatag aggaacaaaa
     8161 tagggcggat attggttttc tgcccgtgct ggtggttagc aatagatgcc atcaccagca
     8221 tcaacaccag gatgggggca acggctttca gtgcgccgac gaacaaagta cctaacagac
     8281 caacagcttc cgccgcgggt tttgagatcc atgccagaag aatccccaga acaaggccga
     8341 ccaggatttg ttttaccagg ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt
     8401 gcgtagtcat ttttcgatcc tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga
     8461 cgcatctttc aggatgtaac aaagtgtttg caaggtcgag tataaggaaa gttacagctt
     8521 cgggaagcga aaaatgctgg attttaaaga gaggtcatat tttgcaactt aattgttata
     8581 tttgtttgac ataaacagag ggaaggactg gctactaagg ctgattagat aacatatggg
     8641 caaaactatt tggaatatga gaacggttgc gctcctttaa tgtgctatgc catataaagt
     8701 cactggcgca gggagcgcgc agggggggcc aatcgccgcc gccccctgca cccccaggct
     8761 ctggcgaaca aaatcgccgc tgcgcgattc cctcagctta tcccttgcgg ctaccgggtc
     8821 gggcgcaagg gaacgtccct gtaagatgcg ccctcgccgc gcatccatgc gcgtcgcccc
     8881 ggcctccagg gaacgcttcg gcgattttga cgcaaccaac acccgtgcgg tctttttgta
     8941 aagagctaca agccaattac ataattaata gctcaccttt gtctggaatg agttatctct
     9001 aggaatggct taatatacag cgaatcattc tccccgctgc ttatcatgcc gataattcac
     9061 ccaggcgttg ataataaacg tcatcaccag aatgccaaac accacgccca gcgagacggc
     9121 gattggaata tggtagaagt cgacaatcag catcttgata ccgataaaca ccaggatcac
     9181 cgccaggcca tatttgagca tcgagaaacg ctctgctacg cccgccagca ggaaatacat
     9241 cgcacgcagg ccgaggatcg caaacaggtt tgaggtcagc acaatgaacg ggtcagtggt
     9301 cacggcgaag atagccggaa tgctatccac ggcgaaaatc acgtcgctca attccaccag
     9361 aatcagtacc agcatcagcg gtgtggcgta cagcaatcca ttcttacgca caaagaaatg
     9421 ctcgttgtcg atggtgtcgg tcatgcgcaa atgaccgcgt agccagcgca ccagcggctt
     9481 gtcgccaatg cctgattcat cttcatgggc gagcgccatc ttcacgccgg taaacagcag
     9541 gaaggcaccg aagatataca ggatccagtc gaactgcgaa attagccagc tgccagtgaa
     9601 gatcatgatg gtacgcagaa caatcgcccc gagcacgcca tacaccagca cgcggcgttg
     9661 taatgccgcc ggaacagaga aatagctgaa caacatcagc cagacaaaga cgttatcgac
     9721 cgccagcgat ttctcaatca gataacccgt gagaaaggcc agtgcctgtg gttcggcaac
     9781 ggcgcgacct tcggtttgca ccagatacca ccagaaagcg gcgttaaaca gtaacgacag
     9841 cgtcacccaa accagcgacc aggccgcagc ctgtttcatg gtcatggcat gtgccccacg
     9901 acgcccctgc aacaacaggt cgatagccag cataatggcg acaacaacag cgaatccgcc
     9961 ccatagcaac ggcgtgccga cagtattcat agaagttcct tacacataaa aaaaacgggc
    10021 aacgtcggaa gacgtcagcc gctgcttttt atgcatagac ctcgccttcc ggcaaggtct
    10081 cacttacaac aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg gtgaccggat
    10141 gtggtttttc acacatcgta atgacgatcg accggcaatg aagttactcc cctttgcagg
    10201 taacaaagta tgacagacca ttcagccagt caatggtctg ttacatacct tttacactgt
    10261 tttactttgg caattatagt tttacgctat ctgccggaaa aatcacccca gtctggcggc
    10321 ggatctcagt cagcagtttg gcggtgatgc gactgaccgc cagccctgga tgatccacca
    10381 gatgctcatc caccagggta gcaaacagct ctgcttcgta gagcatggta ttaatatgct
    10441 gcggctgggt gagatcctgc atctggctgc cacgcggcac gaagcacact ttctggcatt
    10501 cagacagttt ttcaatcacc agcgatcccg cttcgccctg aatctcgctt tccaggacag
    10561 aatcactgac ctttgagtgc agcaacgtaa cgctgaaatc accgtaatcc atcaccacca
    10621 cgccgtgggc atctacgccg cttgccaaca aactggcggt tgcctgcacg cttttcggtt
    10681 caccgaataa tgccaccgcc gacgccaggc agtaaaagcc gatatccata attgaaccgt
    10741 tagagaatgc cggattaaag gtgttgggat tctcaccatc caggtaacgt tgataccgcg
    10801 aggaatactg gcaatagttg aaaaagactt tacgcaattt gccgactttc ggcagcgcct
    10861 ggcgcagcaa atgaaagttc ggcaggctgg cggttttaaa tgcctcaaac agcaccacct
    10921 gattttcccg cgcacaggca atggcggcat ccacttccgc cagattcgac gccagtggtt
    10981 tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg
    11041 cattcgggct ggcaatatac accgcgtcaa tggcatcgct ttccgccatc gcttccagcg
    11101 aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg ctgggcctgt tcaaggctgc
    11161 gggaatatac ggcggttaac ttgtatttac cgctctcatg ggcggcctcg acgaactggc
    11221 gagtgatcca gttcgtacca atcacagcga aacgtatcat aacgcgtgga tactccataa
    11281 agggattctg tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca gatacgcatt
    11341 attcttttgc cggacagcca tgctcactaa acggcccaat catgaccgat ggcccgagat
    11401 atttcggctg gcgatggagg attttggcgt caatcacggc gctgacacga gcgagtttct
    11461 cccgcagttg agtataaaaa ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag
    11521 cgtcgatgct gtactgtttt gccagccaga tggcgcgttg gttatgaaat tcttgcgaga
    11581 taatggtgat atggttttca ccaaaaacct ttttggcacg cactaccgaa tccagcgttg
    11641 agaatccggc atagtcgcag aagatcactt tcgccggtac gcctttagcg atcaaagcct
    11701 tctgcatacc tgattcttca tcgtaatttt tacgtccgtt atcgccactt accagtagcc
    11761 atttcacttt tccggcgtgg tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc
    11821 gattacccgg cctggccccc agcaataagc ccgcgttacg cgccggaact gcgttgacat
    11881 cgctccaggt cagctgcttg ctggcgttga ccattactcg atcggcgata aaaatggtcg
    11941 ctcctgccac aagtaacagt aagcaggcaa tctttagagt ccggcgagaa aaacaaatac
    12001 ggagaagaag gcggggctgc atggtcgtta tccttaacga aaggggggag gtcgatttta
    12061 ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc
    12121 gacaactgcg ttagccacag ccgggtgtcg aactccagct ggtggtactg cggctccata
    12181 tggcagcaca actggtaaaa cgctttgtta tgctctttct ccttcaggtg cgccagttcg
    12241 tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc gaaacacagt agcgacgcgg
    12301 atctctgctt ttgctttcag cttgccgccc agcacacgag aaacagcggt atgtaacccg
    12361 agcgcgtttt ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca
    12421 ttgcgcagaa actgattttt tagatcctga gtatattgcc agagggcttt atcggtggcg
    12481 tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc
    12541 agcgttcgca cctgggaaag tagctgctcg ggataaccct gaagataagt aagattgctc
    12601 ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt
    12661 cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga
    12721 cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcgg
    12781 atgaatattt gctgcaacag ttggacgaca cagaaatccg cggcccggtg ttgatcctga
    12841 atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg
    12901 actcatacat cagtgaactg gcgacgcgcg agaatttacg cctcaacggg attgatgagt
    12961 cgagcgtgaa gtttctcgac agcaccgccg actacccgca agagccgggt gtggtgctga
    13021 tcaaagtgcc gaaaacactg gcattgctgg aacaacaact gcgtgcgctg cgcaaagtgg
    13081 tcacgtcgga tacacgtatt attgccggtg ccaaagccca tgacattcac acttccacgc
    13141 tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc
    13201 gcctgattaa ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct
    13261 ggaagctgga aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg
    13321 ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga
    13381 ttgtcgatct cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc
    13441 aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg
    13501 ttgaaaccaa catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct
    13561 ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa cccgccgttt caccaacaac
    13621 atgcgctgac cgataacgtc gcctgggaga tgttccacca tgcccgccgc tgcctgaaaa
    13681 tcaacggcga gctgtatatt gttgccaacc gtcacctgga ttacttccat aaactgaaga
    13741 agattttcgg caactgcacc accatcgcca cgaataataa atttgtggtg ctgaaagcag
    13801 tgaagctggg gcgtcgtcgg taagattggg agttacctgc cggatgcggc gttaacgcct
    13861 tatccggcct acgttcagca cgttactccc aggccggata agacgcggca agcgtcgcat
    13921 ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa aacaaaaaat ccctctcacc
    13981 atcctctaca ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa
    14041 ttcatacgtt gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg
    14101 atgaagaatg cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca
    14161 acataaaacg gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc
    14221 tgagtcatta aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta
    14281 tgttttcaat gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca
    14341 aaagtgctac atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca
    14401 tcgtactaag gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta
    14461 actgcggtgg caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg
    14521 gaactggtag atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt
    14581 gccaaaataa ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct
    14641 caagccatgc ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc
    14701 cttgccgatc tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag
    14761 aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc
    14821 ttgtttattg attaacgcga cgtgccggat gcgttgcatc cggcgcttca aataacgatt
    14881 taaatctcca gcgccagccg tgttccctgg gcaattgccc gtcgtgcgtc cagctccata
    14941 gctacatcgc agccgccaat taaatgcaca gttttcccgc tatcaatcag cggttgcgcc
    15001 agcgcgcggt ttggctcttg ccctgcgcag atcaccacat tgtccacagc taatacctgc
    15061 gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa tcttctgata acttacgcct
    15121 gggatcattt tcacaccccg cgagagcagg gtggtgcgat ggatccagcc ggtggttttg
    15181 cctaacccct gtcccggttt gctggctttg cgctggagca tcacaatttg ccgtgggcta
    15241 cgcgggatct gcattccctg cgggcttaag ccaccagcct gttgtaggct gctgtcgatc
    15301 ccccactcat tacagaacct ggcgatattc tggctggtgg attcgcccgg ctgacttaaa
    15361 tacatcgccg tatcaaaacc aatcccgcca caaccgatga tggcaacctt gttgcctacc
    15421 ggcgctttgt cgcgcagtac atcgagataa ctcaatacct tcggatgatc gatcccgtcg
    15481 ataggcggaa cgcgcggcac gatcctactg gcgaggatcg tttcatcgaa cgcctgtaac
    15541 tgatccgccg tcacggtgtg attgagtttc agcgtcacgc ccgtcacttc gatcatccgg
    15601 tggtaatagc gcagcgtttc gtaaaactcc tctttgccgg ggatctgttt ggcgatatta
    15661 aactgcccgc cgatctcgct gtgggcatca aacaatgtta cctgatgccc acgcgccgcc
    15721 gcgttaatgg caaacgccag cccagcaggt cccgcaccga ccaccgccag attttttttc
    15781 tgcacggcag gaaggattgg cattttggtt tcgtggcagg cgcgaggatt caccaggcac
    15841 gaggtgactt tgccaacgaa gatctgatcg aggcaggcct gattgcagcc aatgcaggtg
    15901 ttgatctcat cggctcgccc cgattgcgct tttgacagca gttccgcatc agcaagaaac
    15961 ggtcgtgcca tcgataccat atcggcatcg ccgcgcgaga gaatatcgtc ggcaacctgc
    16021 ggatcgttaa tccggttggt ggttaccagc ggcagcgaga cgtggccttt cagtttgcgc
    16081 gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcaa tggtcggaat acgtgcttca
    16141 tgccagccaa tgccggtgtt gataatggtc gcgcccgccg cttcaatggc ctgcgccagc
    16201 tctaccgttt cggcaaaagt cccgccgtct tcgaccaggt cgagcatcga cagtcggtag
    16261 ataataatga agtcgttgcc gacgcgttcg cgcaccgcac gcactacttc tacggcaaat
    16321 cgcatccggt tgcggtaatc gccgccccac tggtcactac gctgattggt gtgcagcgtc
    16381 agaaattcgt tgatcaaata cccttcggac cccattacct ctacaccgtc gtatcctgcc
    16441 tcccgtgcca gttgcgcgca gtgggcgaaa tcgtcgatca gttgcaggat ctcttcatgg
    16501 gtgagttcgt gaggaacgaa acggttgatg ggggcctgca atgcggaggg agcgaccaga
    16561 tgcggttggt agctgtagcg cccggtatgc aaaatttgca gggctatttt gccgccttcc
    16621 tgatgtaccg cttcggtaat ggtgcgatgg tgtgggatct ggctggcgtc gttgagcatt
    16681 gcgccacctt ccatgccaac gcctgttaaa tctggtgcaa tgccgccgct gacaatcagc
    16741 gccacgccgt gacgggcgcg ttcggcataa aacgctgcca gccgctcggc accgtccggg
    16801 tattcctcca gcccggtgtg cattgagccc atcaacacgc ggttttttaa cgtggtaaaa
    16861 cctaaatcca gcggggcgaa cagcgacggg tagctcataa aattgtccag tatgtaaaat
    16921 aattgttatg tggtcggatg agttctaatt tagccgtcac cggagaaaag ggaaaagggg
    16981 agtgcggggt ttgtgatggg attcaaaaag aatacgcccg gtggtaacgc cggatgcgac
    17041 gcttgccgtg ttttatccgg cctgggggat tgcacatgta gggcggataa ggcgtttacg
    17101 ccgcatccgc cagcagttgc aaattattgc ttacggaaaa aatcgttata caacatatac
    17161 aaatgcgcgg cactccagga gaaatttggt gcgccttgct gcgcgccagt cagcgggttg
    17221 taattctcct gaattgggcc atcggcggtt aatcctttgg cgtgccggaa gaacgtatcc
    17281 gccagcttca gggcatcatc acgataaccg taacgctcca tccctttcag agcaaaccag
    17341 aactgatcca cccatacgcg cccgcgccag tagatatcag cgccaaaagc cggattggtt
    17401 aacgccgccg ttcccagcgg gacaaaggtg ttgaactctt taggatcgag catcaccttc
    17461 accaccgcgt cggcattggc ctgcgttgcc gcaccgttaa acagcggcga ccagccttcc
    17521 ggccctttac cgcgctcaac aatcggtttg cccgcgcagc cgttcgccag tggtttatct
    17581 tcaatacgta cgtcatagta gaactgggta gtcgggtcga acatacaggt gttgatgtag
    17641 tccgcgagct gctgtgccaa ctggcgatag cgtttggcct cttctggctt accgaggatg
    17701 gttgccatct ccgccagata atggttatcg ctgtacatat agctggcctg atccaccgac
    17761 tcctgcaata gcgagtagcc cagcaacgtt ccgtcctgac tgcggttttc ggcgaatttc
    17821 accgtccagt cgctacgttt gccaccatta gcgacatatt tatccaactg ttctttgtcg
    17881 ataaaaccaa agacggcggc atcatcacgc cccgattccc acgacgcagc gacctgtgcc
    17941 ggaatttcca gactgtcgta ctggcctttc tccaccacgc gggcgtagtt gttcagccca
    18001 gactgcgtct cttctttgtt gcctttcttc accgcaaaca gcatctcgcc gctctcagtg
    18061 ttgtgggctt tgtcgcgggt cgcgccatat tccggcacgc cgttgccgtt gtgatcgcgg
    18121 ttacgtaacc accagtcgtg ataggccacc agtttcgggt acatctctgc caaccaggct
    18181 ttatcctggg tgacgttgta cacttctatc accgaccagg cggcaaggct gggtttggta
    18241 ttacgttcgt tccagttgcc gccatcgcca ccacgctcgg ggctaaggtt ccacgctatc
    18301 aggtcgggga caaagcccac atcctgcgga cgcacgcggt cgccaggctg gatctgccag
    18361 gagaagaccg cgcggatatt ctctttggcg atgtccggat tgaaatgcgc catcgcaaac
    18421 gcctgcttct aggtatccca cggccaggtc tgattgccgg agaaccagcg cccggtcacc
    18481 gacggtgtaa cggtgttaaa tttcaccgca ccgcccggtg agcgccagtt accgttgagc
    18541 gtttcgatcg ctttcaccgc gacgcgcgtc tgttccggcg tcgcatccgg attggttaac
    18601 cctttcttca ggtattcttc ccagcgttgc tgcgagtcgg tgagataaaa cgccggacgc
    18661 gccagaatat cgcggatctg catttgctct ttgctaactt cctgagcggt cagcagatgg
    18721 gaataggtgg tgtagagcgt ggtcgaaccg ttgatatgcg ccttactggt aaagcgatta
    18781 ccgttgattt cagtctgcac cggcagggat ttatgcacct gatattccga ttcgccggag
    18841 gtcagcagat cccaggtggc gcgcactttg ccaaaggtga ctttcaggcc atcacgggtg
    18901 gcgctgattt tgcgctgata gtcagggtat tcgccagcaa tggttttatc ggaaagcggt
    18961 ttcccttctt tcgcttccag tttttccagc agttcgccgt cccacaccag atccagcggt
    19021 ttattgctgg tgattttggt ttccagtagt gacgtgcgcg gcgtggcgaa gcgcagagtc
    19081 atttcgactt gcacatcttt tgctgtcagt ttttgcacca gcgcaccggg aatactgtat
    19141 gcctccagcg taaagtcgac tttcttgccg tcctgccaga cggtcaggcg gtcgaaattg
    19201 ctggccataa agttgatgta ctcttccgtc agcagcgcaa cgcccggaaa gccgcccatg
    19261 gtgttagggc cgtctggcaa cagatgacca tgccaggctc cgagatcgaa aaacggatta
    19321 aaacgctggt gatcgtcgta atcgtaatcc ttcatgtact gcggcgcgcc agtacggtta
    19381 atcacgtttt tataattgtc ggcgttagcg gcatgggcgg aaaaacttat cagcagagcg
    19441 caggttactg gcgttaaaat agtttttatt ttcatgaaat tacagtcctt attaccagta
    19501 gaaatattgc atcaggaaga tttggtcgct gggatcgtga gaggcatttt tcatgtagaa
    19561 gcggctatca aaagccgtgg cgaaacgtcc gccgccgtat tcgtaccaga taccaaactg
    19621 catgaaagag gtggtttcat cgtcagcgtg gtcagggcga tgtttggcgt aggtgtaagc
    19681 ggtggagaga taaacgccct ttaacgcttc atacatggcg ctcaccatca tcccactttc
    19741 acttcccggt acgtccagcc cgtcggcgcg tccggtatgg tgccaggcgc gagcggcaaa
    19801 gttaggagtt agcaggcccg tgagatagag ctgtcctgtg ccgtcggtga tttccagacc
    19861 gttcatccag gtgatgcctt cggcaaggtc atactggatg taaccgttca tcattgccgg
    19921 ataggtgtat ttgtcgtcat agcgatcgta tttgccaaaa tgtagccagg ctttgctttc
    19981 atcatgatgt ccggcaggag tcgcggtgac gctataacga aagttgccgc ttaagttctg
    20041 taccttcagc gcagtcataa tgtcgcgggt attgggaatg acgtagccca gatcgggggt
    20101 gaaatcgccc caccattgca ggtcatccag cgaggaatcc tgacgcagac tgagcatgat
    20161 ttcagtgcca tcattggtac gataaccgcc atagaaacgg ttaattccgc cctcgaaacc
    20221 accccagcta tggtctggca cccaggcatt gccgtcgtgg tcggcctgca cggtccagcc
    20281 ttcgccgtaa atcaggccga accaggagcc gtacttcatc tccaggccac cttcaatata
    20341 ggtaccgtcg ctgtatttat gtttattctc acc
//
LOCUS       DAJTMK010000066        20271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTMK010000066 DAJTMK010000000
VERSION     DAJTMK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20271
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.067"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..121)
                     /locus_tag="OO135_002028"
     CDS             complement(<1..121)
                     /locus_tag="OO135_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose specific IIB subunit"
                     /protein_id="HCR5397875.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIP"
     gene            complement(149..592)
                     /gene="cmtB"
                     /locus_tag="OO135_002029"
     CDS             complement(149..592)
                     /gene="cmtB"
                     /locus_tag="OO135_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR5397876.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIGFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(906..2897)
                     /gene="tkt"
                     /locus_tag="OO135_002030"
     CDS             complement(906..2897)
                     /gene="tkt"
                     /locus_tag="OO135_002030"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5397877.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKDAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            3175..3933
                     /gene="loiP"
                     /locus_tag="OO135_002031"
                     /gene_synonym="yggG"
     CDS             3175..3933
                     /gene="loiP"
                     /locus_tag="OO135_002031"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5397878.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4139..5059)
                     /gene="speB"
                     /locus_tag="OO135_002032"
     CDS             complement(4139..5059)
                     /gene="speB"
                     /locus_tag="OO135_002032"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5397879.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5196..7172)
                     /gene="speA"
                     /locus_tag="OO135_002033"
     CDS             complement(5196..7172)
                     /gene="speA"
                     /locus_tag="OO135_002033"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5397880.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVSTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7181..7312)
                     /gene="yqgB"
                     /locus_tag="OO135_002034"
     CDS             complement(7181..7312)
                     /gene="yqgB"
                     /locus_tag="OO135_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5397881.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7448..7663
                     /locus_tag="OO135_002035"
     CDS             7448..7663
                     /locus_tag="OO135_002035"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397882.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            7967..9121
                     /gene="metK"
                     /locus_tag="OO135_002036"
     CDS             7967..9121
                     /gene="metK"
                     /locus_tag="OO135_002036"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5397883.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            9557..10951
                     /gene="galP"
                     /locus_tag="OO135_002037"
     CDS             9557..10951
                     /gene="galP"
                     /locus_tag="OO135_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5397884.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRYTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11028..11525
                     /locus_tag="OO135_002038"
     CDS             11028..11525
                     /locus_tag="OO135_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5397885.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVVK"
     gene            11620..12327
                     /gene="endA"
                     /locus_tag="OO135_002039"
     CDS             11620..12327
                     /gene="endA"
                     /locus_tag="OO135_002039"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5397886.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            12407..13138
                     /gene="rsmE"
                     /locus_tag="OO135_002040"
     CDS             12407..13138
                     /gene="rsmE"
                     /locus_tag="OO135_002040"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5397887.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13151..14101
                     /gene="gshB"
                     /locus_tag="OO135_002041"
     CDS             13151..14101
                     /gene="gshB"
                     /locus_tag="OO135_002041"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5397888.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTCTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIAHQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14210..14773
                     /locus_tag="OO135_002042"
     CDS             14210..14773
                     /locus_tag="OO135_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5397889.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPERRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14773..15189
                     /gene="ruvX"
                     /locus_tag="OO135_002043"
     CDS             14773..15189
                     /gene="ruvX"
                     /locus_tag="OO135_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5397890.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(15365..16345)
                     /locus_tag="OO135_002044"
     CDS             complement(15365..16345)
                     /locus_tag="OO135_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5397891.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTILPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            16363..17067
                     /gene="yggS"
                     /locus_tag="OO135_002045"
     CDS             16363..17067
                     /gene="yggS"
                     /locus_tag="OO135_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5397892.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17085..17651
                     /gene="yggT"
                     /locus_tag="OO135_002046"
     CDS             17085..17651
                     /gene="yggT"
                     /locus_tag="OO135_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5397893.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGLWMAL"
     gene            17648..17938
                     /locus_tag="OO135_002047"
     CDS             17648..17938
                     /locus_tag="OO135_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5397894.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17946..18539
                     /locus_tag="OO135_002048"
     CDS             17946..18539
                     /locus_tag="OO135_002048"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5397895.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            18532..19668
                     /gene="hemW"
                     /locus_tag="OO135_002049"
     CDS             18532..19668
                     /gene="hemW"
                     /locus_tag="OO135_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5397896.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGRKVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(19823..20191)
                     /locus_tag="OO135_002050"
                     /pseudo
     CDS             complement(19823..20191)
                     /locus_tag="OO135_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
BASE COUNT         4893 a         5308 c         5214 g         4856 t
ORIGIN      
        1 tgggaataaa taacgcagta ataaaacccc aggcaataaa agcaccaata ttggggatga
       61 ccattgcagt caaaaagccc ccaaaagcct ggacctttgc acgagcagac ttgttttcca
      121 taatattatc ctgtagagga gagagtaatt atccgcggct gataatgtcc gctaattgtt
      181 tttctgatga tgctgttaag agttgttcga gtatttcttc ttcgcacagt aattcactta
      241 acgcctgaat agcgccaatg tgcgaatcgg catcggcagc agagagtccg atgagtaatt
      301 taattggttc gtcattcccc ggaaaataaa caccttgttc aagtaatgtc aaagacatcc
      361 cggttttaag cgccccacat tccggtcgcg catgaggcat tgccacgcct ggtgcgagaa
      421 tataataagg gccattgtta atggtggaat ctttaattgc ctgaatgtaa ttctcgctga
      481 tatagttttt atccagcaat gataccatcg agaaaccgat agcttcctgc caatcttttg
      541 ctgaatgtat aactgagatt gatgattctg gaaaatagtc actaagccgc ataaattatc
      601 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      661 aaagcaattg agcagggaaa ccggtgtttt gaaaacagtg aattattaat ttcttatata
      721 acattgagtt ataggtataa caacaaatgt caatacgcat atcgtgatgc atattatata
      781 attgatattt attgcatata aatatttgtg atctgcaaca cgcttcatct attgcttgtc
      841 cgcaaacgga catatcaagg taataaaaaa ggtcgccgaa gcgacctttt tacccgaaat
      901 gctaattaca gcagttcttt tgctttcgca acgacgttgt cgacggtgaa gccgaactct
      961 tcgaacagca actctgccgg agctgactca ccgaaggtgg tcataccgat gatagcgcca
     1021 ttcaggccaa catacttgta ccagtagtca gcaatacccg cttctaccgc aacgcgtgca
     1081 gtaaccgctt tcggcagtac ggattcacgg taagcagcat cctgcttgtc aaatgcgtcg
     1141 gtagacggca tggacaccac gcgcgctttc acgccttcgg cagtcagttt ttcgtaggca
     1201 gcaacagcca gttcaacttc tgaaccggta gcgatgaaaa tcagttccgg ctgaccggcg
     1261 cagtctttca gcacataacc accgcgcgcg atgtttgcca gttgctcttc agttcgttcc
     1321 tgctgcgcca ggttctgacg ggagaggatc agtgcggtcg ggccgtcctg acgctcaaca
     1381 ccgtatttcc acgcgactgc ggattcaacc tggtcacacg gacgccatgt agacatgttc
     1441 ggggttacgc gcagagaagc gacctgctct accggctggt gagtcgggcc atcttcgccc
     1501 agaccgatgg agtcgtgggt gtaaaccatc acctgacgct gtttcatcag cgcagccata
     1561 cgtacggcgt tacgtgcgta ttccacgaac atcaggaagg tggaggtgta cggcaggaaa
     1621 ccaccgtgca gggagatacc gttagcaatc gcggtcatac cgaactcgcg aacaccgtag
     1681 tggatgtagt tacccgcagc atcttcgttg attgctttag aaccagacca cagagtcagg
     1741 ttagacggtg ccaggtcagc agaaccgccg aggaattccg gcaacagcgg accgaaagct
     1801 tcgatagcat tctgagacgc tttacggctg gcgattttcg ccgggttagc ctgcagttta
     1861 gcaatgaact cttttgcttt cgcgtcgaag tcagacggca tttcgccttt catacggcgg
     1921 gtaaattcag cggcttcctg cggataagct ttcgcgtaag cagcgaattt ctcgttccat
     1981 gcggattctt tcgcctggcc tgcatctttc gcatcccact gagcatagat ttcagacggg
     2041 atttcgaacg gtgcatattt ccagcccagc tgttcgcggg tcagggcaat ttcagcgtcg
     2101 cccagcggcg caccgtggga gtcgtgagta ccggctttgt tcggggaacc gaaaccgatg
     2161 atggttttgc acatcagcag ggacggtttg tcagtcactg cgcgcgcttc ttctactgcg
     2221 cgtttgatgg atgccgcgtc atgaccgtcg atgtcgcgaa taacgtgcca gccgtaagct
     2281 tcgaaacgca ttgcggtgtc gtcggtgaac cagccttcaa cgtgaccatc aatagagata
     2341 ccgttgtcat cgtagaacgc aatcagttta cccagcttca gcgtacccgc cagagagcaa
     2401 acttcgtggg agatgccttc catcatgcag ccgtcgccca tgaaggcgta ggtgtagtgg
     2461 tcgacaatgt cgtggcccgg acggttaaac tgcgccgcca gcgttttttc tgcaatcgcc
     2521 ataccgactg cgttggcaat accctgaccc agcggaccgg tggtggtttc cacaccagcg
     2581 gtgtaaccca cttccgggtg gcccggagtt ttagagtgca gctgacggaa gtttttcagt
     2641 tcttccatcg gcagatcgta accggtgagg tgcagcaggc tgtagatcag catggagccg
     2701 tggccgttgg acagcacgaa gcggtcacgg tcagcccagg acggattctg cgggttgtgt
     2761 ttcaggaaat cacgccacag gacttcggca atgtcagcca tacccatagg ggcacccggg
     2821 tgaccggatt tggctttctg tactgcgtcc atgctcagcg cacgaatagc attggcaagc
     2881 tctttacgtg aggacatttt gactccagat cggatgatga agggcacgcc cttaacgact
     2941 tgacgacagc gcgttttggg ctacgccgga aaatttgcca acaatttacc gcaagccgcg
     3001 cgtcatgtac atggaacatc cttttgccgc ttcagaaatc tctggatcat gctcgcatgt
     3061 tgcgcaatct actcgcccgt ccgctgcgct tttccttata ctgagactga gcgtcgattc
     3121 acctgcaaac ggcgcatttt tagaataatc ctgaccttgt gcggaagaga aaacatgaaa
     3181 attcgcgcct tattggtagc aatgagcgtg gcaacggtac tgactggttg ccagaatatg
     3241 gactccaacg gactgctctc atcaggagcg gaagcttttc aggcttacag tttgagtgat
     3301 gcgcaggtga aaaccctgag cgatcaggca tgtcaggaga tggacagcaa ggcgacgatt
     3361 gcgccagcca atagcgaata cgctaaacgt ctgacaacta ttgccaatgc gctaggcaac
     3421 aatatcaacg gtcagccggt gaattacaaa gtgtatatgg cgaaggatgt gaacgccttt
     3481 gcaatggcta acggctgtat ccgcgtctat agcgggctga tggatatgat gacggataac
     3541 gaagtcgaag cggtgatcgg tcacgagatg gggcacgtgg cgttaggcca tgtgaagaaa
     3601 gggatgcagg tggcgcttgg tacaaatgcc gtgcgagtag ctgcggcctc tgcgggcggg
     3661 attgtcggca gtttatctca atcacaactt ggtgatctgg gcgagaaatt agtcaattcg
     3721 caattctccc agcgccagga agcagaagcc gatgattatt cttacgatct tctgcgccaa
     3781 cgcggcatca gcccggcagg tcttgccacc agctttgaaa aactggcgaa actggaagaa
     3841 ggccgccaaa gctcaatgtt tgacgaccat ccggcatccg ccgaacgcgc ccagcatatt
     3901 cgcgatcgca tgagcgcgga tgggattaag taaagcctgg tggtgtcgaa cgtaggtcag
     3961 ataaggcgtt cacgccgcat ctgacattaa tggcacattt gttaccttgt gcgcattgcc
     4021 ggatgcgatg ctggcgcatc ttatccggcc tacgggtgcc gaacgtaggt cggataaggc
     4081 gttcacgccg catccgacat taatggcacg ttttacccgt gcgcatcgca tctggtgctt
     4141 actcgccctt tttcgccgcc tgaatataca gcatctccag cgccagcgtc gccgcagcca
     4201 gagcggtgat ttccgactga tcgtatgccg gagccacttc cactacgtcc atcccaacaa
     4261 tgttgagatc tttcaggccg cgtaccagtt taatggcgcg atcggaggtc aggccgccaa
     4321 tcactggcgt accggttccc ggcgcgaaag caggatccag gcagtcgata tcaaaggtca
     4381 ggtaaaccgg catatcaccc acaatctgtt tcacttgggc gataacgtca tccacgctgc
     4441 gatcgttcac ctggcaggcg tccagcacgg taaaaccgtt gtctttatca aactcggtac
     4501 gaataccaat ctgcacggaa tgattcgggt cgatcagacc ttctttcggc gcggtataga
     4561 acatggtgcc gtggtcaaat tcacaaccgt tcgcgtaggt atcggtgtgg gcgtcaaagt
     4621 gtaccagcgc cattttgccg aaatgcttcg catgagcacg cagcagcggc agcgtaacaa
     4681 agtggtcacc accgaaagag agcatacgct taccggcagc cagcagcttc tcggcgtgtg
     4741 cctgcagttt ttcgctcatc tcacgggcat cgccaaaggc atataccaga tcgccgcagt
     4801 ccacgacgtt caggcgctca cgcatgtcga aattccacgg gaagcgattg tgttcccagg
     4861 caagattcgt cgaaacctga cggatcgctg ccggaccgtg gcgtcccccc gcacgaccag
     4921 aagtggccat atcgaacggc acaccagtaa tcacccagtc agcatcgctg tcatacggct
     4981 ggaagttcat cggcaggcgt aaaaaaccaa aggcgttgga aaccagtgag ttatcgtatt
     5041 gatgacctaa ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccttc
     5101 cgcgtcgtta ggcccgacga ggaagggttg gatttgtcac aataaattgt ggcggattat
     5161 caccgctaaa ttaagcggat tcaagtaata cggacttact catcttccag ataagtataa
     5221 ccgtacaaac ctgcctcgaa ctcttcaagg aactgttgtt gcagttcagc atcaagatcg
     5281 gttttcttca cttgatcgcg gaactgagtt aacagcgttt tcggatcgag ctgcacatat
     5341 tgcagcatat ccgcaacggt atcgccttcg tcagacagtt ccacttctac actaccgtca
     5401 gggaagacga acacgtcaac cgcttcggta tcaccgaaca ggttgtgcat gttgccgagg
     5461 atctcctgat atgcgccgac cataaagaaa ccgagcatcg gcggattctc tggatcgtac
     5521 tccggcattg gcattgtcgt ggcaatacca tcaccatcaa tatagtggtc gatagcaccg
     5581 tcagagtcac aggtaatatc cagcagcaca gcgcgacgtt ccggcacttg atccagccct
     5641 tccagcggca gaaccgggaa caactggtcg atcccccatg cgtccggcat tgactggaac
     5701 agcgagaagt tgacgtacat tttgtccgcc atacgttcct gcagttcgtc gattatcgga
     5761 cgatgcgcac ggttttgcgg atccagttgc ttctgcactt catggcacat gctcagatag
     5821 agttgttctg cccatgcacg ttcttgcagg ctaaaggtgc cggaagagta gccgatatga
     5881 atgtcgtgca gatccatctg actgtcgtgt aaccattcac gcagagaacg gcgagttccc
     5941 ggttcgtgca tctcctgcca ggtttcccac atgctttgca gcgcgcgcgg cgcatcttct
     6001 gcaggcgcgg tcgacaccgt gtattcgtta cgttccacgc cgatgatatt agacaccagc
     6061 acggtgtgat gcgcagtcac cgcacgaccc gattcggtga ttaccgtcgg atgcggcaga
     6121 ccgttttctt cacacgcatc accaatcgcc cagatgatgt tgttggcata ttcattgagg
     6181 ccgtagttta cggagcagtc agactgcgaa cgggtgcctt catagtccac gcccagaccg
     6241 ccgccgacgt cgaagcactg aatattgacg cccagcttgt gcagttccac atagaaacgc
     6301 gcggattcac gaacgcccgt ggcgatatcg cgaatattcg ccatctgcga accgaggtgg
     6361 aagtgcagta gttgcaggct gtcgagacgc ccagcttcac gcagcgtttc aaccagttgc
     6421 agtacctgag tcgcagccag gccgaacttc gatttttccc cgccggaaga ctgccattta
     6481 cccgagccct gcgaagccag acgtgcacgc acgcccagac gaggaacgac attcagacgt
     6541 tctgcttcat ccagcacaat ggcgatttct gacatcttct caatgaccag atagaccttg
     6601 tgccccatct tctcgccaat taatgccagg cggatatatt cgcggtcttt ataaccgttg
     6661 cagacgatga cgctacgggt catgccagca tgtgccagca ctgccatcaa ctcggctttg
     6721 gaaccggctt ccagacccag cggttcgccc gaatgaatca gggactcaat cacgcggcgg
     6781 tgctggttaa ctttgatcgg ataaacaagg aagtaatcgc cgttatagcc gtaggattcc
     6841 ctcgcacgtt tgaacgcggc gttaatggaa cgcaaacggt gctgcaggat ctgtgggaaa
     6901 cagaacagtg caggcagacg ctgaccctgt gcttcacgag ttttcactaa ctgcgcgaga
     6961 tcgacgcgag cttccgggac gtccgggtcc gggcacacgc tgatgtggcc cagctcgtta
     7021 acgtcatagt agttattgcc ccaccaggca atattgtaag tacgcagcat cttgctggct
     7081 tcctgggagc tcattgcaac ctcctgcatg gagcgtagta caccgtgttc gcccgctgac
     7141 gaaggcaaac ccatagacat gtcgtcagac atagcgaacc tcaaattatt ttattaagtg
     7201 taaaacagtt aacgactatc gcagcctgga actgcgataa caacccataa gcacatggat
     7261 tttccagcag tgaatgctga cgctccaact gcgcaaccgg tttctttttc ataacattat
     7321 taagcacata accgaacgta agtgtgacag ttcggcgaaa ccacgagaaa actcttgttt
     7381 ttacaagagc gcccttgttc agtcctcagt aactgtaacc agctcttgaa tcctgagaag
     7441 cgccgagatg ggtataacat cggcaggtat gcaaagcaga gatgcagagt gcggggaacg
     7501 aatcttcacc agaacggtga gacaggttaa gcagcagact acggttcatt atttcgtatc
     7561 acctccacgg ccgcctgtta agacgaaccc acaagccaaa actctgattt caacccggct
     7621 ggaagtggca acacgaaaga aacgtcgtgt gctttttatt taagccgcgc gccgcgtttt
     7681 ataccccaca atggcagaaa attgcaaaat ataaatacgc agaatgccgg cattgtcagg
     7741 aaaaatttcc agccacgttt ttaacagaat gagacacgat tcaaaaaaaa gtggaaatcg
     7801 ggtgaagaat tgacctaaaa tagccatcca gatgttaatc catccatacc gattaacact
     7861 cagactgcca gtgtttttaa tctgcagagt cgtggtagga tccgctacca cagaaaatcc
     7921 acacaacagt ttgagctaac caaattctct ttaggtgata ttaaatatgg caaaacacct
     7981 ttttacgtcc gagtccgtct ctgaagggca tcctgacaaa attgctgacc aaatctctga
     8041 tgccgtttta gacgcgatcc tcgaacagga tccgaaagca cgcgttgctt gcgaaaccta
     8101 cgtaaaaacc ggcatggttt tagttggcgg cgaaatcacc accagcgcct gggtagacat
     8161 cgaagagatc acccgtaaca ccgttcgcga aattggctat gtgcattccg acatgggctt
     8221 tgacgctaac tcctgtgcgg ttctgagcgc tatcggcaaa cagtctcctg acatcaacca
     8281 gggcgttgac cgtgccgatc cgctggaaca gggcgcgggt gaccagggtc tgatgtttgg
     8341 ctacgcaact aatgaaaccg acgtgctgat gccagcacct atcacctatg cacaccgtct
     8401 ggtacagcgt caggctgaag tgcgtaaaaa cggcactctg ccgtggctgc gcccggacgc
     8461 gaaaagccag gtgactttcc agtatgacga cggcaaaatc gttggtattg atgctgtcgt
     8521 gctttccact cagcactctg aagagatcga ccagaaatcg ctgcaagaag cggtaatgga
     8581 agagatcatc aagccaattc tgcccgctga atggctgact tctgccacca cattcttcat
     8641 caacccgacc ggtcgttttg ttatcggtgg cccgatgggt gactgcggtc tgactggtcg
     8701 taaaattatc gttgatacct acggcggcat ggcacgtcac ggtggcggag cattctctgg
     8761 taaagatcca tcaaaagtgg accgttccgc agcctacgca gcacgttatg tcgcgaaaaa
     8821 catcgttgct gctggcctgg ccgatcgttg tgaaattcag gtttcctacg caatcggcgt
     8881 ggctgaaccg acctccatca tggtagaaac tttcggtact gagaaagtgc cttctgaaca
     8941 actgactctg ctggtacgtg agttcttcga cctgcgccca tacggtctga ttcagatgct
     9001 ggatctgctg cacccgatct acaaagaaac cgcagcatac ggtcactttg gtcgtgaaca
     9061 tttcccgtgg gaaaaaaccg acaaagcgca gctgctgcgc gatgctgccg gtctgaagta
     9121 atctttcttc acctgctttc aaaggccagc ctcgcgctgg cctttttctt ttacctcacc
     9181 gcggtggata ggcattcacg ccgcatccgg caaaaagctg cccgcacaat aacatcattc
     9241 ttcctcaaca cgttttatcg caaattatca tcactactga aaccgattac accaataaca
     9301 ccagccaaat atttgtaatg ttttcatatt attattcagt tttcacagtt gttacatttc
     9361 ttttcagtaa agtcttaatt gcagataaca gcgtttaatc tatgatgata taactcaatt
     9421 attttcatgc acttaaacca taactaagat aaatgttagt gtaagcgatt acactaatgt
     9481 gatttgcttc acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac
     9541 catattggag ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac
     9601 gtttttcgtc tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt
     9661 aattgctggc gcactgccgt ttattgcaga tgaattccag attacttcgc acacgcaaga
     9721 atgggtcgta agctccatga tgttcggtgc ggcagtcggt gcggtgggca gcggctggct
     9781 ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc gcaattttgt ttgttgccgg
     9841 ttcgctgttc tctgcggctg cgccaaacgt tgaagtactg attctttccc gcgttctact
     9901 ggggctggcg gtgggtgtgg cctcttatac cgcaccgctg tacctctctg aaattgcgcc
     9961 ggaaaaaatt cgtggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct
    10021 cggtgcttat ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg
    10081 tgtgattatc atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc
    10141 acgttggttt gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg
    10201 ttacaccagc gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa
    10261 acagagtggc tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg
    10321 cgtactgtta caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc
    10381 gaaaatcttc gaactggcgg gttataccaa cactaccgag caaatgtggg ggacagtgat
    10441 tgtcggcctg accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg
    10501 acgtaaacca acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg
    10561 tacaatgatg catatcggta ttcactctcc gtcagcgcag tatttcgcca tcgccatgct
    10621 gctgatgttt attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc
    10681 cgaaattcag ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg
    10741 gattgccaac atgatcgttg gcgcaacgtt cctgaccatg ctcaacacac tgggtaacgc
    10801 caacaccttc tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct
    10861 ggtaccggaa accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg
    10921 taaactgcgc gaaataggcg ctcacgatta atctccccaa gcttcctccc atcgcggagg
    10981 aagccacctc ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc
    11041 gtctccctat cgccatccaa caggccgtta tgcgtcgcct gcgggaaaaa ctcgcccagg
    11101 ctaacctgaa attggggcgt aactacccgg agccaaaact ctcttatacc cagcgcggca
    11161 cctccgccgg aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt
    11221 tggaaaacag tgaagccttt attgaagaag tggtgccgca cgaactggca catttgctgg
    11281 tatggaaaca tttcgggcgc gtagcaccac atggcaaaga gtggaagtgg atgatggaaa
    11341 acgtgctggg tgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca
    11401 acaccttccc ctaccgctgc aagtgccagg agcatcagct taccgtacgc cgccataatc
    11461 gcgtagttcg tggcgaagcc gtctatcgct gtgttcactg cggcgaacaa ctggtcgtaa
    11521 aataaccatc tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact
    11581 ttcgctacgt tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt
    11641 gctgcggtgg tactgagcgc agcattttcc ggcccggcgt tggccgaagg tatcaatagt
    11701 ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg acgcgcccgg tacgttttat
    11761 tgcggatgta aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc
    11821 tatcaggtgc gcaaaaatga aaaccgagcc agccgcgtag agtgggaaca cgtcgttccc
    11881 gcctggcagt tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct
    11941 aaagatccgg tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggt
    12001 gaggtgaatg gcgatcgcgg caactttatg tacagccagt ggaatggcgg cgaaggccag
    12061 tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt
    12121 gcacgcggtg ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc
    12181 tctcgccagc aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg
    12241 gagtgcgagc gcgatgaacg catcgcgaag gtgcaaggca atcataaccc gtatgtgcaa
    12301 cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg gactcttttt gttaacccct
    12361 accccacgcg tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg
    12421 catttatcat cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc
    12481 caaccatatc gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg
    12541 tagcaaccag gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa
    12601 ggtgctggaa ggcaagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt
    12661 gatgtcgcgt ggtgaaaaaa tggaatttac gatccagaaa tcgatcgaac tcggtgtaag
    12721 cctcattacg ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa
    12781 caagaagctc cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa
    12841 ccgggtgccg gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga
    12901 aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgccgttacc
    12961 ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc
    13021 catgactgcc cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga
    13081 gacaactgcg ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg
    13141 gagaagaata atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa
    13201 gaaagattcc agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta
    13261 tatggagatg ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cctgcacgct
    13321 gaacgtgaag cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct
    13381 ggccgatctc gatgtgctcc tgatgcgtaa agacccgccg tttgataccg agtttatcta
    13441 cgcgacctat attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca
    13501 gagcctgcgc gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga
    13561 aacgctggtt acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat
    13621 cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga
    13681 tccaaacctc ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc
    13741 gcaaaattac ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga
    13801 gccggtaccg tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc
    13861 tgccggtggt cgcggtgaac ctcggccgct gacggaaagt gactggaaaa tcgcccatca
    13921 gatcgggccg acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga
    13981 ccgtctgact gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt
    14041 tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta
    14101 acccacctta gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg
    14161 catactgggc gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca
    14221 gcatcacttt cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt
    14281 ctacatttgc gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga
    14341 aaatctcaaa attgaaggga ttctggaaaa gctgaagatc acgccggagc ggcgtgatga
    14401 atcaatccgt ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt
    14461 tattttgcat actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt
    14521 gatgaccact tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt
    14581 attggtggct ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga
    14641 taacgcgtgg ctaacggccc cggcggatct gaatattttg ttcaaaacgc cgattgccga
    14701 ccgctggcgc gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc
    14761 agggcacgcc tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc
    14821 gtagcggtcg gccaacgcat taccggcact gctcgccctt tgcctgcaat taaagcacag
    14881 gacggtacgc cggactggaa cctcatcgaa cgtttactga aagagtggca gccggacgaa
    14941 atcatcgtcg gtttgccgct gaatatggac ggcaccgagc aaccattgac tgccagagcg
    15001 cgtaaatttg ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgaa
    15061 cgtcttagca ccgtggaagc ccgttccggt ctgtttgaac agggcggcta tcgggcgctc
    15121 aacaaaggca aagtcgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag
    15181 ggatattaag gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg
    15241 gcgtgaacgc cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta
    15301 gcgtcgcatc agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc
    15361 aacgtcacaa acgcccttcc cccacccgct gctgataact ctgctgaaac gttatcatcc
    15421 ccacctgctg cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca
    15481 aattccccac cgcgggagtg ttaatcaata attcaaacag cgccacgcgt ccttcctgtt
    15541 tatccacttc cagcttttgt gacaacacgg cccgtaaact acctgccagt tgattacgta
    15601 cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac
    15661 cacgcgtatg taatgttgcc agcaccaaat gcccggtttc tgccgccgtc agtgccagac
    15721 ggattgtctc gctgtcacgc agctctccga gcaaaatcac atcaggatct tcccgcaatg
    15781 cggcccgcaa tcccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca
    15841 aacatcgctg gctggcatag atatattcaa caggatcttc cagcgtcaga atatgcgcat
    15901 cggcatgttg attgagatag ccaaccatcg ccgccagcgt ggtagatttg ccgctccccg
    15961 tcgcccccgt caccagaatc aggccattct cgctcttgag taattccggc aatatcgttg
    16021 gtgcgccaag ctgttcgagc tgcgggcagt gcgaaggtaa cagccgtaac gccagcgaaa
    16081 tgccatgccg ttgcgcgaat gcgctgccac gcaatcgctg gttttccgcc agcgacacgg
    16141 caaaatccag ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc
    16201 gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc ttccattagc ccgcgaatgc
    16261 gccatcgtgc gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa
    16321 gggccacaat ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat
    16381 aacctggcac aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca
    16441 gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc
    16501 attgatgccg ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt
    16561 cgccactttc aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag
    16621 tctaataaaa gccgcctggt ggcagagcat ttcgactggt gtcataccat cgaccgtttg
    16681 cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg
    16741 attcaaatta atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac
    16801 gagctggcag cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc
    16861 cctgcgcctg agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca
    16921 tttgccggac tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac
    16981 gatatggaag ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt
    17041 ggtgcgcgtg attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt
    17101 tcctgctttc aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc
    17161 agtgggctca ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtaacgcagc
    17221 caattatcgg gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc
    17281 tgctggttgc ctatattctc agttttatca aagccattgt gctgtttaaa gtggtgacct
    17341 tcctgccaat catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga
    17401 ttttctgggt cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga
    17461 ttgaatacgt gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac
    17521 cggcaatggg tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca
    17581 atatgggtgt cgcagaagta ttacaggcaa ccggaaacat cctgctgccg gggctgtgga
    17641 tggcgttatg aatgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca
    17701 gccgaaagcc agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat
    17761 taccgcgccg ccagttgacg gccaggccaa cagtcatctg gtgaagtttc tcggtaagca
    17821 attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca
    17881 aattaaaatc attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt
    17941 atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt
    18001 cgctgcttag cgactttggt cttgatatcg tggcacaaac agacctcggc gttgattccg
    18061 ctgaagaaac cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa
    18121 aagtgaccgg tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg
    18181 gcgcgccggg tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc
    18241 tgcaaaaact gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcacgtttcc
    18301 actgcgtgct ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca
    18361 gctggccggg cgtgattact cgcgaaccag cgggcactgg tggctttggt tatgatccaa
    18421 tcttcttcgt accttccgaa gggaaaacag ccgccgaact gacccgcgaa gaaaagagcg
    18481 ccatttccca ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa
    18541 ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc
    18601 gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg
    18661 ctttgcgatc tggacaatga tgtggcttac gctcagggcc gtaaagtaaa gacaatcttt
    18721 attggcggtg gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctggacggc
    18781 gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaaccctggt
    18841 acggtagaag ccgatcgctt tgtcgattat cagcgtgctg gtgtgaaccg catctctatt
    18901 ggtgtgcaga gttttagcga agaaaagctg aaacgacttg ggcgcattca tggcccgcaa
    18961 gaagcgaaac gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat
    19021 ttgatgcatg gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc
    19081 attgaactga atccgccgca tctttcctgg tatcaactga ccatcgaacc caatacgctg
    19141 tttggttcgc gaccaccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag
    19201 gggcatcagt tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa
    19261 cccggttatc agtgccagca caatctcaac tactggcgat ttggtgacta catcggtatt
    19321 ggctgcggcg cgcacggcaa agtcaccttc ccggatgggc gcattctgcg taccactaaa
    19381 acgcgtcatc ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa
    19441 gccgcagata agccgtttga gttctttatg aaccgctttc gcttgctgga ggccgcgcca
    19501 cgcgcagagt ttagtgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac
    19561 gaggcgattg cccagggtta tctcacagaa tgtgcggatt actggcagat aacggaacat
    19621 gggaagctgt ttttaaattc gctgctggag ctttttctgg ctgagtaaac ttgtattgcc
    19681 ggatgcggcg tgaacgcctt atccagccta catgtggcaa cggttgtagg tctgataaga
    19741 cgcgcaagcg tcgcatcaga cgttgattgc cggatgcggg gtcaacgccg catcctgcta
    19801 caaatcgtgc actatatcaa acttacttaa tcaccccatt gcgccgcagt tgttcggtat
    19861 cttttttcca gcgttcatta gcttggttga tatcgtcaat tttcatcaaa atcgcgactt
    19921 ttttactgtt tatttctgaa actttcatcg ttaacggata ttgccctttt tcgagcgtta
    19981 cccagtcatc ctgcaattta cggctgagtt tttgtgattg cagaaaagtc tgtcgccgtt
    20041 gttcgttgat atcgaaatct cgtttctgct gaatcggcac ttcataacac tcttcactct
    20101 gtttatgaca tgccgcaatc gccggttgat aaacctcttt gtcatattta accgcgaacg
    20161 cctcgctatc gttctgttta ttaaactcat cagtaagcgt acagcctgaa ccgtctggtc
    20221 agaatctgac aaattagaca aagtggtgtc caccaaataa gtagtgggaa c
//
LOCUS       DAJTMK010000067        19662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTMK010000067 DAJTMK010000000
VERSION     DAJTMK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19662
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.068"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(95..907)
                     /locus_tag="OO135_002051"
     CDS             complement(95..907)
                     /locus_tag="OO135_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5397897.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            1169..1930
                     /gene="udp"
                     /locus_tag="OO135_002052"
     CDS             1169..1930
                     /gene="udp"
                     /locus_tag="OO135_002052"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5397898.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2071..3498
                     /gene="rmuC"
                     /locus_tag="OO135_002053"
     CDS             2071..3498
                     /gene="rmuC"
                     /locus_tag="OO135_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5397899.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3593..4348
                     /gene="ubiE"
                     /locus_tag="OO135_002054"
     CDS             3593..4348
                     /gene="ubiE"
                     /locus_tag="OO135_002054"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5397900.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4362..4967
                     /gene="ubiJ"
                     /locus_tag="OO135_002055"
     CDS             4362..4967
                     /gene="ubiJ"
                     /locus_tag="OO135_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5397901.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            4964..6604
                     /gene="ubiB"
                     /locus_tag="OO135_002056"
     CDS             4964..6604
                     /gene="ubiB"
                     /locus_tag="OO135_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5397902.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6683..6952
                     /gene="tatA"
                     /locus_tag="OO135_002057"
     CDS             6683..6952
                     /gene="tatA"
                     /locus_tag="OO135_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5397903.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDSDFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            6956..7471
                     /gene="tatB"
                     /locus_tag="OO135_002058"
     CDS             6956..7471
                     /gene="tatB"
                     /locus_tag="OO135_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5397904.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7474..8250
                     /gene="tatC"
                     /locus_tag="OO135_002059"
     CDS             7474..8250
                     /gene="tatC"
                     /locus_tag="OO135_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5397905.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8292..9074
                     /gene="tatD"
                     /locus_tag="OO135_002060"
     CDS             8292..9074
                     /gene="tatD"
                     /locus_tag="OO135_002060"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5397906.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(9071..9559)
                     /gene="rfaH"
                     /locus_tag="OO135_002061"
     CDS             complement(9071..9559)
                     /gene="rfaH"
                     /locus_tag="OO135_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5397907.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9726..11219
                     /gene="ubiD"
                     /locus_tag="OO135_002062"
     CDS             9726..11219
                     /gene="ubiD"
                     /locus_tag="OO135_002062"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5397908.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11265..11966
                     /gene="fre"
                     /locus_tag="OO135_002063"
     CDS             11265..11966
                     /gene="fre"
                     /locus_tag="OO135_002063"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5397909.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12158..12976)
                     /locus_tag="OO135_002064"
     CDS             complement(12158..12976)
                     /locus_tag="OO135_002064"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HCR5397910.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARMHG
                     ISREMQDAFAARSHARTWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVE
                     ALATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGC
                     DPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKIN
                     LNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(12986..15175)
                     /gene="fadB"
                     /locus_tag="OO135_002065"
     CDS             complement(12986..15175)
                     /gene="fadB"
                     /locus_tag="OO135_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5397911.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIVKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15365..16696
                     /gene="pepQ"
                     /locus_tag="OO135_002066"
     CDS             15365..16696
                     /gene="pepQ"
                     /locus_tag="OO135_002066"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5397912.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            16696..17310
                     /locus_tag="OO135_002067"
     CDS             16696..17310
                     /locus_tag="OO135_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5397913.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17349..18800
                     /gene="trkH"
                     /locus_tag="OO135_002068"
     CDS             17349..18800
                     /gene="trkH"
                     /locus_tag="OO135_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5397914.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            18812..19357
                     /gene="hemG"
                     /locus_tag="OO135_002069"
     CDS             18812..19357
                     /gene="hemG"
                     /locus_tag="OO135_002069"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5397915.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4581 a         4968 c         5377 g         4736 t
ORIGIN      
        1 tccaatcaat tcatactgat tattttttgg gggacagaga tggcagcaaa acgcactcgt
       61 tcactcacca tctcaaatat agactaagag gaaactaact ccgcttccca ccatactgct
      121 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
      181 gataatcagc gttgaatgca tgcccggcgt ccgggtacac gataatctct gctttcgcat
      241 tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg ctgttatcct
      301 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
      361 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
      421 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
      481 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
      541 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
      601 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
      661 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
      721 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
      781 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
      841 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
      901 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
      961 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagccctgca gaatgaagag
     1021 tgatttatgt gatttgcatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     1081 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     1141 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     1201 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     1261 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     1321 cacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     1381 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     1441 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     1501 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     1561 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatccattg gcgcgacaac
     1621 tcacgttggc gtgacagctt cttctgatac cttctaccca ggccaggaac gttacgatac
     1681 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     1741 cgtaatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa gtcagggcct
     1801 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     1861 tgagacgatg aaacaaactg aaagccaagc ggtgaaaatc gtggtggaag cggcgcgtcg
     1921 tctactgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     1981 gtagcgcctc gcaggaaatg cctttccaac tggacgcttg tacagcacaa ttctattttg
     2041 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     2101 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     2161 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     2221 caaattaccc aaagcgagca ctggcgtgca gagtgcgagt tactcaataa cgaagtgcgc
     2281 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     2341 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     2401 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     2461 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     2521 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     2581 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     2641 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
     2701 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
     2761 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
     2821 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
     2881 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
     2941 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
     3001 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
     3061 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
     3121 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgcaa cgcccagcaa
     3181 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
     3241 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
     3301 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
     3361 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
     3421 cgacttcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga tgatgaatat
     3481 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
     3541 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
     3601 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
     3661 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
     3721 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
     3781 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
     3841 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
     3901 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
     3961 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
     4021 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
     4081 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
     4141 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
     4201 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
     4261 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
     4321 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
     4381 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
     4441 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
     4501 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
     4561 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
     4621 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
     4681 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
     4741 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
     4801 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
     4861 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
     4921 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc caggtgaagt
     4981 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
     5041 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
     5101 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
     5161 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
     5221 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
     5281 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
     5341 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
     5401 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
     5461 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
     5521 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
     5581 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
     5641 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
     5701 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
     5761 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
     5821 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
     5881 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
     5941 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
     6001 ttcaggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
     6061 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
     6121 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
     6181 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
     6241 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgcgct
     6301 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
     6361 attggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg ataagattgc
     6421 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
     6481 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
     6541 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
     6601 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
     6661 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
     6721 cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
     6781 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
     6841 cagtcaggat tctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
     6901 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
     6961 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
     7021 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
     7081 ggcgacaacg gtgcagaacg aactgatcca ggagttaaaa ctccaggagt ttcaggacag
     7141 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
     7201 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatccaga
     7261 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
     7321 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
     7381 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga aaaccgctgc
     7441 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
     7501 atcacgcatc taattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
     7561 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
     7621 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
     7681 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
     7741 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
     7801 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
     7861 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
     7921 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
     7981 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
     8041 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
     8101 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
     8161 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
     8221 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
     8281 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
     8341 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
     8401 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
     8461 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
     8521 ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
     8581 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
     8641 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
     8701 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
     8761 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
     8821 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
     8881 aattgctgat cgaaactgat gcgccgtatc tgctcccgcg cgatttcacg ccaaagccat
     8941 catcccggcg caacgagcca gcccatctgc accatatttt gcaacgtatt gcgcactggc
     9001 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
     9061 ggattgcgtt ttagagtttg cggaactcgg tattcttcac actgtgctta atctctttat
     9121 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
     9181 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa ggggttgccg
     9241 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
     9301 tcgctggcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg ttgatagtcg
     9361 tggtatgaat cacttctgga tcaaattcga caaacaggta gttgggaaac aatggctcac
     9421 tgactgcagt acgttttcca cgcacgattt tttccagggt gatcatcggt gccaggcaat
     9481 tcacagcctg tctttcgagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
     9541 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
     9601 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
     9661 atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgta gattaagagg
     9721 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cttgacgctg cttgaacagc
     9781 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
     9841 ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
     9901 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatgggac
     9961 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    10021 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caagtattga
    10081 acatgccgac aaagcggctg cgtggtgtgc cctgccaaca aaaaatcgtc tctggcgatg
    10141 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcaccgctga
    10201 ttacctgggg gctgacagtg acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    10261 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    10321 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    10381 tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt ccggatacac
    10441 tttcagagta tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg gtgaagtgta
    10501 tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg tatatcgaac
    10561 aaggcgaaac tgcgccggaa gggccgtatg gcgaccacac cggttactat aacgaagtcg
    10621 atagtttccc ggtatttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    10681 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    10741 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    10801 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    10861 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    10921 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    10981 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    11041 attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc acgaataaat
    11101 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtcg
    11161 cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    11221 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    11281 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    11341 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    11401 aacgtccgtt ttcaatggct tcgacgccgg atgaaaaagg gtttatagag ctgcatattg
    11461 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagattatc
    11521 aaatcgtggt cgatattccc tacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    11581 tgattttgat tgcgggcggc accgggttct cctatgcgcg ctcgatttta ttgacggcgt
    11641 tggcgcgtaa tcccaatcgc gatatcacca tttactgggg tgggcgtgag gagcaacatc
    11701 tttatgatct ctccgagctt gaggcgcttt cgctgaagca tcctggcctg cgtgtggtgc
    11761 cggtggttga acaaccggaa gcgggctggc gtgggcgtac tggcaccgtg ttaacggcgg
    11821 tcttgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    11881 tggcgaaaat tgctcgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgtctgt
    11941 ttggcgatgc gtttgcattt atctgagata taaaaaaaac cgcccctgac aggcgggaag
    12001 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaatcggtg
    12061 cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc aggatcggta
    12121 gaccggataa gacgttcacg ccgcatccgg caagtggtta aacccgctca aacaccgtcg
    12181 caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg tctttgcgtt
    12241 ccatcagatt cagcagcgtg gtgctgatac gcgcaccgga acagcccagc ggatgaccca
    12301 gcgcgatcgc accaccattg aggttgatct tctcgtcaat ctgctccatt agcccgagat
    12361 ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac acgccgatat
    12421 cgctggcaga aagccccgct tttttcagcg ccagcttcga ggccggaacc gggccgtaac
    12481 ccataatcga tgggtcacaa ccaacgaccg ccatcgaacg cacacgagcg cgcggcttaa
    12541 gacctaattc atgggcgcgg ctttcactca tcaccagcat ggcagctgcg ccatcggaaa
    12601 gtgcagaaga tgtgcccgcc gttaccgtac cgtttactgg atcaaacgcc ggacgcagcg
    12661 tggcgagggc ttccacggtg gtttccggtc gaatcacctc gtcgtaatta aactgcttca
    12721 ggacgccgtc ggcatcgtga ccaccggtcg ggatgatttc atttttaaat gcgcccgact
    12781 gcgtggcggc ccaggtgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc
    12841 tgataccgtg catacgcgcc agcaggctac gcattaaatg agcggagaga tcttctgcac
    12901 gcacgttacg aaaagcaccg cccttcgaac ggcccatcgg ggtgcgaatt gcatcgacaa
    12961 tgacaacctg ttccattgtg actccttaag ccgttttcag gtcgccaacc ggacgggctg
    13021 gctcaaccgg aggatagtac ggttcgttat gacgcgcttt attacgcaga ccttccggca
    13081 cttcatacag cgggccgagg tgctgatatt gctgtgccat atcgaggtat tttgcgctac
    13141 cgagggtgtc cagccagcgg aacgcgccgc cgtggaacgg agggaagccc aggccgtaga
    13201 ccagcgccat atccgcttcc gccggagtgg cgataatgcc ctcttccaga cagcggacca
    13261 cttcgttgac catcgggatc atcatgcggg cgataatctc ttcttcgctg aaatcgcgct
    13321 tcggctggct cacttctgcc agcaggtctt caacggcggc gtcttcttct ttcttcggct
    13381 tacctttgct gtcttcttta taacgccaga aaccgaggcc gttcttctga ccaaagcggt
    13441 tggcatcaaa cagcgcgtcg atggcatcgc ggtaatcttt ctgcatccgc tgcgggaagc
    13501 ctgctgccat gacagcctga gcgtgatgcg cggtatcaat gcccacgaca tccagcagat
    13561 atgccggacc cataggccag ccaaactgtt tttccatcac tttgtcgatc ttgcggaaat
    13621 ccgcgccgtc gcgcagcagc tggctgaaac cggcgaaata cgggaacagc acgcggttaa
    13681 caaagaagcc ggggcagtcg ttaaccacaa tcggcgtctt gcccatcttg ctcgcccagg
    13741 cgacaacttt cgcgatggtt tcgtcggagc ttttctcgcc gcgaataatt tctaccaacg
    13801 gcatccggtg taccgggtta aaaaagtgca ttccgcagaa gttttccggg cgttccagcg
    13861 cgttggccag ttcgctgata ggaatggttg aagtgttaga cgccagcacg gtatccgggc
    13921 gtactttttg ttcggtttct gccagtacgg cttttttcac tttcgggttt tcgacaaccg
    13981 cttctaccac aacatccacg cggtcaaacc cggcgtagtc gagcgttggg tggatcgtgg
    14041 agatcacgcc agccagtttc agaccatcga tcttgccgcg ttcaagctgc ttgttcagca
    14101 gtttcgcggc ttcggtcatg ccgagggtta acgacttgtc gttgatatct ttcatgacaa
    14161 ccggtacgcc tttccacgca gactggtaag caatgccgcc acccatgatg cctgcgccca
    14221 gtacagcggc ctgtttcggt gtttcaacgt ctttggtgag tttcttcgct ttgcctttta
    14281 catattgatc gttaaggaaa atgccgacca gtgcgcgggc ttcgttggtg tgcgccagcg
    14341 ggacaaagct tttgttttcc agatttaatg cttcttcacg accaaaacgg gccgcagctt
    14401 caatggtttt tactgcggtg atgggcgccg gataatgttt ccccgccgtt tgtgcgacca
    14461 tccctttaac gatggtgaag ctcatggtgg cttcaatctt gctcagtttt agtggttcca
    14521 gcttcggctg acgttttgct ttccagtcga ggtcgccgtt aatggcctgg cgtaaaaccg
    14581 cctttgcgcc ttcaaccagt ttttctgctt tgactacgcc atccaccaga ccgattttca
    14641 gcgcctgatc cgcgccgaca tctttaccgg cggcaatgat ttccagcgca ctgtcagcgc
    14701 ccagcatacg tggcatacgt acagaaccgc caaagcccgg catgatgccc agtttggttt
    14761 ccggcagacc gatgcgcaga tccggcgtcg ccagacgata atcggtcgcc agcacgcatt
    14821 cgcagccacc acccagcgca tagccgttga cggcagcaat ggtcggcacc ggcagatctt
    14881 ccaggcgatt aaacacgcta ttggcaaagt gcagccactg acttaactgt tcttcaggaa
    14941 cgaggaacag ggacaaaaat tcggtgatat cagcaccgac gataaaggct gctttgttcg
    15001 aacgcagcag cagccctttt agatctgatt gctgttccag cacgccgatg gcctcgccga
    15061 ggctggcgac ggtcgcagtg tcgagtttat taactgaacc tggggcatca aataccagtt
    15121 cggcaatgcc atcttccagc cagtcaaggt acagggtgtc gcctttgtaa agcatgtcag
    15181 tctcctgaat ccgcaaggtg atctggtcgt accagatgca tcgaagtgtg tattttatgt
    15241 taaaaatatg caaataaaag attaaaaaaa tgccgatctg atcacgctcg gcagaaatca
    15301 cgctctggat gaacgatgtg ctaagatgcg gagacttaag gtcaaaaaaa cagaagggta
    15361 aaaaatggaa tcactggcct cgctctataa aaatcatata gctaccttac aagaacggac
    15421 tcgcgatgcg ctggcgcgct tcaagctgga tgcattactt attcactccg gcgagctgtt
    15481 caacgttttt ctcgacgatc atccctatcc gtttaaagtg aacccgcaat tcaaagcgtg
    15541 ggtgccggta actcaggtgc caaactgctg gttgctggtg gatggcgtga acaagccgaa
    15601 actgtggttc tatctgccgg ttgattactg gcacaacgtc gaaccgctgc cgacctcctt
    15661 ctggactgaa gatgtggaag tgatcgcact gccgaaagcc gatggcattg gtagcctgct
    15721 gcccgctgcg cgcggcaata tcggttatat cggtccggtg ccggaacgtg cgctgcaact
    15781 gggtattgag gccagcaaca tcaacccgaa aggggtgatc gactacctgc attactaccg
    15841 ctccttcaaa accgagtacg aactggcctg tatgcgtgaa gcgcagaaaa tggcggtcaa
    15901 cggtcatcgt gcggcagaag aagcgttccg ttctggcatg agcgagttcg atatcaacat
    15961 cgcctatctg accgcgaccg gtcatcgtga taccgacgta ccttacagca acattgtggc
    16021 gcttaacgaa cacgcttcgg tgctgcatta caccaaactg gatcatcagg caccggaaga
    16081 gatgcgcagc ttcctgctgg atgccggggc cgaatataac ggctatgcgg ctgacctgac
    16141 tcgtacctgg tcggcaaaaa gcgacaacga ctacgcacag ctggtgaaag acgtaaatga
    16201 tgaacaactt gcgctgatcg ccaccatgaa agctggcgtc agctatgtgg attaccacct
    16261 ccagttccat cagcgcatcg ccaaattgct gcgtaaacat caaatcatca ccgatatgag
    16321 tgaagaagcg atggtcgaaa acgatctcac cggaccgttt atgccgcacg gtatcggcca
    16381 tccgctgggc ctgcaggtgc atgacgtagc cggttttatg caggatgata gcggtacaca
    16441 cctcgcggca ccggcaaaat atccgtacct gcgctgcacc cgtattctcc agccgggcat
    16501 ggtgttaacc atcgaaccgg gtatctactt catcgaatcg ctactggcac cgtggcgtga
    16561 agggcagttc agcaagcact tcaactggca gaaaattgaa gcactgaaac cgttcagcgg
    16621 cattcgtatc gaagacaacg tggtgatcca cgaaaataac gtggaaaaca tgacccggga
    16681 tctgaaactg gcgtgatgga aagctggtta attcctgcgg caccggtcac ggtcgttgaa
    16741 gagatcaaaa agagccgctt cataacgatg ttggcgcata ccgatggcgt tgaggcggcg
    16801 aaagcgtttg ttgaatcggt gcgggcagaa caccccgatg cccgccacca ttgcgtggcg
    16861 tgggtggcgg gtgcgccgga tgattctcaa cagctgggtt tctctgacga cggggagccg
    16921 gcgggaacgg caggtaaacc gatgctcgcc cagctaatgg gcagcggcgt cggggaaatt
    16981 accgctgtgg tagtgcgcta ctacggcggc atattgcttg gcaccggtgg gttagtgaaa
    17041 gcgtatggcg gcggcgtgaa tcaggcgctg cgccagctaa cgacccaacg caagacgcca
    17101 ttaaccgaat atactttgca atgtgaatat agtcagttaa ccggcattga agcgttgctg
    17161 gggcagtgtg acggcaaaat tatcaacagt gattatcagg cattcgttct gctgcgggtg
    17221 gcgcttccgg cggcgaaagt ggctgaattt tccgcaaagc tggcggattt tagccgtggt
    17281 tcattgcaat tgttagcgat tgaagaataa tccccacttc gttttgcaga actaaggaag
    17341 cggcagagat gcattttcgc gccattaccc gaatcgttgg actactggtc atcttatttt
    17401 cagggaccat gattatccct gggctggtag cactcatcta ccgggatgga gcgggccgcg
    17461 cttttaccca gacctttttt gtcgccctcg ccattggctc tatgctgtgg tggccgaacc
    17521 gcaaagagaa gggcgaactg aaatcccgtg aggggtttct gatagtggtg ctgttttgga
    17581 ccgtgctggg tagtgtcggt gcgctccctt ttatcttctc ggaaagcccg aacctcacga
    17641 ttaccgatgc gttttttgaa tctttctctg gcctgaccac caccggggcc actacgctgg
    17701 tggggctgga ttcgctccct catgccatcc tcttttatcg ccagatgctg caatggtttg
    17761 gcgggatggg gatcatcgtg ttagcggttg cgatactgcc tatcctcggc gtgggtggga
    17821 tgcagctcta tcgcgcagaa atgcccggcc cgctgaaaga taacaaaatg cgcccgcgaa
    17881 ttgcggaaac ggcgaaaacc ctgtggttga tttatgtctt gctgaccgtc gcctgtgcgc
    17941 tggcgttgtg gtttgccgga atggatgcct ttgatgccat cggccatagc tttgcgacta
    18001 tcgctattgg cggcttctcg acacatgatg ccagtatcgg ttatttcgac agtccgacta
    18061 ttaacactat cattgctatc ttcctgctga tctccggttg taactacggt ctgcactttt
    18121 cactgttaag tgggcgtagt ctgaaggttt attggcgcga tccggaattt cgcatgttta
    18181 tcggcgtaca gtttacgctg gtggttattt gtacactcgt actgtggttt cataatgtct
    18241 acagttcggc gctgatgaca attaaccagg cgtttttcca ggtggtatcg atggcgacaa
    18301 ccgccgggtt tacgactgac agcattgccc gctggccgct ctttttgccg gtactgcttt
    18361 tatgttcagc ttttatcggc ggttgcgccg ggtcaacggg cggtggcctg aaagtgatcc
    18421 gcatcctgct gctgtttaag caggggaacc gtgagctgaa acgactggtg catccgaacg
    18481 ccgtctatag cattaagctg gggaatcgcg cactgccgga acgtatcctc gaagccgttt
    18541 ggggattttt ctccgcctat gcattggtgt ttattgtcag tatgctggcg attatcgcca
    18601 cgggcgtgga tgatttttct gcctttgcct cagttgttgc gacattgaat aacctggggc
    18661 cagggcttgg cgtggttgct gataacttta ccagtatgaa cccggtggct aaatggatcc
    18721 tgattgccaa catgctgttt ggtcgtctcg aggtctttac attgctggtg ctctttaccc
    18781 cgactttctg gcgtgaatga tggagtaata cgtgaaaaca ttaattcttt tctcaacaag
    18841 ggacggacaa acgcgcgaga ttgcctccta cctggcttcg gaactgaaag aactggggat
    18901 ccagacggat gtcgccaatg tgcaccgcat tgaagaacca caatgggaaa actatgaccg
    18961 tgtggtcatt ggtgcttcta ttcgctatgg tcactaccac tctgcgttcc aggaatttgt
    19021 caaaaaacat gcgacgcgac tgaattcgat gccgagcgcc ttttattccg taaacctggt
    19081 ggcgcgcaaa ccggagaagc gtactccaca gaccaacagc tacgcgcgca agtttctgat
    19141 gagttcacaa tggcgtcccg atcgctgcgc ggtcattgcc ggggcgctgc gttacccacg
    19201 ttatcgctgg tacgaccgtt ttatgattaa gctgattatg aagatgtcag gcggtgaaac
    19261 ggatacgcgc aaagaagttg tctataccga ttgggagcag gtggcgaatt tcgcccgaga
    19321 aatcgcccag ttaaccaaca aaccgacgct gaaataagca taaagaataa aaaatgcgcg
    19381 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    19441 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaattc
    19501 cgacagagaa agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc
    19561 ccgcgccgct gagaaaaagc gaagcggcac tgctctttaa caatttatca gacaatctgt
    19621 gtgggcactc gaagatacgg attcttaacg tcgcaagacg aa
//
LOCUS       DAJTMK010000068        19572 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTMK010000068 DAJTMK010000000
VERSION     DAJTMK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19572
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.069"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            6..81
                     /locus_tag="OO135_002070"
     tRNA            6..81
                     /locus_tag="OO135_002070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO135_002071"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO135_002071"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="OO135_002072"
     CDS             complement(468..1226)
                     /locus_tag="OO135_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5397916.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2327)
                     /locus_tag="OO135_002073"
                     /pseudo
     CDS             complement(1234..2327)
                     /locus_tag="OO135_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(2337..3507)
                     /locus_tag="OO135_002074"
                     /pseudo
     CDS             complement(2337..3507)
                     /locus_tag="OO135_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3575..4600)
                     /locus_tag="OO135_002075"
     CDS             complement(3575..4600)
                     /locus_tag="OO135_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5397917.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTVLQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5031..5252)
                     /locus_tag="OO135_002076"
     CDS             complement(5031..5252)
                     /locus_tag="OO135_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397918.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5505..8609)
                     /gene="acrF"
                     /locus_tag="OO135_002077"
                     /pseudo
     CDS             complement(5505..8609)
                     /gene="acrF"
                     /locus_tag="OO135_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(8621..9778)
                     /gene="acrE"
                     /locus_tag="OO135_002078"
     CDS             complement(8621..9778)
                     /gene="acrE"
                     /locus_tag="OO135_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR5397919.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10177..10839
                     /gene="acrS"
                     /locus_tag="OO135_002079"
     CDS             10177..10839
                     /gene="acrS"
                     /locus_tag="OO135_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR5397920.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIDWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10842..11021)
                     /locus_tag="OO135_002080"
     CDS             complement(10842..11021)
                     /locus_tag="OO135_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5397921.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11105..11989)
                     /gene="yhdJ"
                     /locus_tag="OO135_002081"
     CDS             complement(11105..11989)
                     /gene="yhdJ"
                     /locus_tag="OO135_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5397922.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLILADPPYN
                     IGKNFDGLIDAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12075..12371)
                     /gene="fis"
                     /locus_tag="OO135_002082"
     CDS             complement(12075..12371)
                     /gene="fis"
                     /locus_tag="OO135_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5397923.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12397..13362)
                     /gene="dusB"
                     /locus_tag="OO135_002083"
     CDS             complement(12397..13362)
                     /gene="dusB"
                     /locus_tag="OO135_002083"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5397924.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13691..14572)
                     /gene="prmA"
                     /locus_tag="OO135_002084"
     CDS             complement(13691..14572)
                     /gene="prmA"
                     /locus_tag="OO135_002084"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5397925.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14584..16035)
                     /gene="panF"
                     /locus_tag="OO135_002085"
     CDS             complement(14584..16035)
                     /gene="panF"
                     /locus_tag="OO135_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5397926.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16025..16267)
                     /locus_tag="OO135_002086"
     CDS             complement(16025..16267)
                     /locus_tag="OO135_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5397927.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16360..17295)
                     /locus_tag="OO135_002087"
     CDS             complement(16360..17295)
                     /locus_tag="OO135_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5397928.1"
                     /translation="MGELLAEFLSRNPHQTFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(17327..18157)
                     /locus_tag="OO135_002088"
     CDS             complement(17327..18157)
                     /locus_tag="OO135_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5397929.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            18500..19354
                     /locus_tag="OO135_002089"
     CDS             18500..19354
                     /locus_tag="OO135_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCR5397930.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQSAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            19390..19566
                     /locus_tag="OO135_002090"
     CDS             19390..19566
                     /locus_tag="OO135_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter"
                     /protein_id="HCR5397931.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKTMSI"
BASE COUNT         5193 a         4893 c         4688 g         4798 t
ORIGIN      
        1 agcgcgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggatcagt cggaagactg ggccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccattt catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggttc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacct
      781 gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttcttaggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta aaacggtcag atgagggaag agattgaaat gctgaaatac cattcccact
      961 tcctgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctacgaca
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaaccagaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta tggacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggcgttaaa
     1261 acgtttttcc agatgttggc tataacgtga catgctgaaa caaaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaagcc gatgatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacacct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaccccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggtagg gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctcaagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttg gtgcgattga cacaaggcca atcagtaatg ccaggttaat tcgccagcgt
     2041 tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat gaagacccaa
     2101 caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccagttagc ctgcaaaaat
     2161 gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc aatagtcagc
     2221 aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc gcggcttgag
     2281 ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat gactccttag
     2341 cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat agtcaggctg
     2401 ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc cgtctggttc
     2461 agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc ggcaagagag
     2521 gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac tcgcagtgcc
     2581 tggggaataa tgacctggcg tagcgtaacc ccagtgaccg agccgcttca tgttgaccat
     2641 aaggcactgc ctggctccca gcgcggataa tctcggcgat aaatgcagag gtataaaccg
     2701 aaagtgccag cgttaaggct gccagttcag gaattaaaac cattccgccg cggaagttaa
     2761 agcctcgtag ggctgggaca tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta
     2821 aaggcaaacc gatgatcaac actgcggcga acggccaggt gcggcgcagt tgtctggtct
     2881 ttatctggta tgttttatta aagcggaata gcccaacaga aaggactata gccataacaa
     2941 cagccaggat aaacgcaata aatccgtctc ccagctgcgg tgacggaata taaagcccac
     3001 gattgctcaa aaaggccaga tcaaacgcgc tcacagcctg gcgcgtggtc cgggcaaatt
     3061 gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc gggggaatat tacggaagat
     3121 ctcaatataa attgtggata gctttcgtag cagccagtta tccgaaagtc tcgccagacc
     3181 gataaagaag cctaaaacag aagcgaacac aatacacaat gcagaaacca gtagcgtatt
     3241 gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc tgctggtaat cgatcaaatg
     3301 ctggacaata ccgaagccag cgccgcgatc cagaaaggca aaacctgaag taatgccacg
     3361 attactgaga ttcgttacag tgttgtgaaa taaccaacca acaattccga caacagcaac
     3421 aacggcaagg atctggaata accaggcacg aaccgtaggg ttggcaaagg agagtgagcc
     3481 tttaacggtt gagcggcgat gagacataga aacctcggaa agcataacgc tgaagagagc
     3541 gccgccgtag cgacgctcgt tacctcacac cgacttaacg cacgggcggc gcgtactgaa
     3601 taccgccgtt attccagaga ttattttgcc cacgtttaat tttcagcggg ctttctgaac
     3661 ctacgttacg ctcgaaaatt tccgagtagt tacccacctg tttgatgatg ttataggccc
     3721 atttattatc caacttcaga tccttgccgt aatcgccttc tttacccagc agatgtgcca
     3781 tatcaggcgt tgctggatta gccgcttttt catcgacgtt ctgggaattg atgcccatct
     3841 cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat tcatcatcgc
     3901 cacgacgaac taccggccca agcggttctt tagagataac ctccggtaag acaatccatt
     3961 cagctgggtt gcttaatttg atgcgcaggg catacagttg cgactgatcc gaggccagcg
     4021 tatcgcagcg accagattcc agtgccttcg ctgattcgtc agagcgatcg aaagtcaccg
     4081 gtgtgtactt catattgttt gccttgaaat agtcggcaac gttgagttcg gtatcagtac
     4141 ccgcctgaat acagacggta gcgccatcca gttctttcgc gctttttagc cccgctttat
     4201 cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc attcccatcc
     4261 cggcgtcgcg agatgaagtc caggtcgtat tacgggagag caaatccact tcccctgact
     4321 gtaaaaccgt gaagcgttct tttgctgtga gtggggtata tttcactttc gtgtcgtcac
     4381 caaatacagc agcggcaaca ctacgacaaa catcaacatc aatacctgaa aacttaccgt
     4441 ctgcatcggc ataagagaac ccaggtaatc catcactgat cccgcattgc acaaaacctt
     4501 ttttctgaac ggcatcaagc gtcgcgccag catgcgcctg atttgcaacg gcaagcagca
     4561 cgctggcggc agccagtgtg gctatcatca tctttttcat aatgcttcct gtgaggcgaa
     4621 aattatcgtt ataggacgtt tgggaaacgt cttttacctg tctgtggcca tcctcttata
     4681 agcaaaagta gtgccagctt ttgcgcacga tttcgcgtcg caataagctg agtgtagaca
     4741 ggaaataata aaaacagcgc cccgcaatgg tgcgaaaatg aaacatgcgc cacattttgg
     4801 cgcaaaatat ttacgcgtgt tttactaagc agaataatga ttgttagcgt gagtatattt
     4861 ctggtgggaa gtgtgaaagt taaattgatg cgttatttcg aaagcagcga aataacgcga
     4921 tataacatgc taaatcagca gagaattagc cagcagaata tagcaatatt ttatcttatg
     4981 taaagatact gtatcgttaa acagagcgcg aaactccgcg ccctgttcag ttatttcgac
     5041 agagcgatga tccccattgt caggccagca acagccgctg ccccgccggc aatagcccca
     5101 gccgtttccc agttatcctg agtcgtgcct ttcatacagg tattggtgtg aaccagacga
     5161 ccctgatcgt cataaaccgg aacgcagggc gaatcgtgag cacagcccgc cagcaatgcg
     5221 gtgagcaatg ccacaggaat taatcttttc atggtgttac ctcagtaata aacgctaacg
     5281 cttaataagt taactctctt attaacgagc tgaatgccat tttatcaccg gaggttataa
     5341 atattgcgga atgaataatg acaaattatg tgaattgtaa aaatagtgaa gggagcggga
     5401 actatagaaa gtttttaaag agtgagtgga agaatatctt attaattttt atgggtttca
     5461 ctgaaaataa taaaggcacc cgaaagtgcc tttatgtttc tgatttatcc tttaaagcaa
     5521 cggcggatca ccacaaagaa caccggtacg aagaagattg ccagcaacgt tgcagagacc
     5581 attccgccca ttaccccaat acccactgcg ttctgcgcgc cactgcctgc accgttactg
     5641 atagctagcg gtaatacgcc gagaataaag gcgagagagg tcatcaggat aggacgcaga
     5701 cgcatacgta ctgccattag tgtcgcttca acaacacctt taccctcttt ctccatgaga
     5761 tctttagcga actcaacaat caaaatagcg tttttggccg acaagccaat tgtcgttagc
     5821 aagcccacca taaagtagac gtcatttttt tgattaaaga gtgttgccgc cagcagcacg
     5881 tcgacaatcc ctaacggcac gaccaacata accgagacag gaattgacca gctttcatag
     5941 agtgcagcaa ggcacaggaa aacaaccaca aaggaaattg ctaccagagc gggagcctgg
     6001 tttcccgata agcgttcctg atacgacata cccgtccagt cataaccaat gcccgcaggt
     6061 aattttgacg caaggttttc catcaacgcc atggcatcgc cggaactggt tcctggcgcg
     6121 gcttccccct gaatctccat tgacggcaga ccgttgtagc gttccagtcg cggagagcca
     6181 tacacccaat gtgaagtggt aaaggccgag aatggcacca tttcgccgtt ggcgctgcgg
     6241 acataaagtt tatcgacatc ttctggcagc atacggaatt tggcatccgc ctgaacatac
     6301 acctttttca cgcgaccacg gtcgatgaag tcgttaacat aagtcccacc caacgccgtt
     6361 gaaatggtct gattgatgtc agaaagtgaa acacttaatg cctgcgcctt ttcctggtca
     6421 acttccagtt taaactgcgc ggtgtcttcc aggccattag gacgcacgct gactaagctg
     6481 gcaggatgtt gcgccgccat accaagcaac tggttacggg cctgggttag ggcatcgtga
     6541 cccagcccag cctgatcaat taactcaaag tcgaaacccg ttgccgtgcc cagttcaaca
     6601 atggctggca tattgaatgg aatgacaaaa ccgtcgcgga tcttgcccaa ttccattttg
     6661 gcacgatgga ttaccgcttc cgcactgttt tcatcaccat tacgctcttc ccacggtttc
     6721 agactgacga aggccatacc ggcgttttat gcctggccgc tgaagctaaa gccgttaacc
     6781 gtaaagacac tttcaacgtt cgctttctcg ttcttcagat agtaatccgt cacttgatcc
     6841 aacacttttt gcgtccgctc ttgcgtcgcg ccagcgggta actgaatcat ggtcagaaag
     6901 acaccctgat cctcttcagg taagaaggaa gacggaagac gtaaaaacaa caccaccatt
     6961 cctgcaacaa tcagcgcata gatcagtaaa tatcgtcctg tggaaccgag gattttgccg
     7021 acgctgttgg tgtagtggtt aacgctatga tcgaaggtgg tattaaacca gccgaagaaa
     7081 ccgcccttat tttcgtgatg ctcagcagag acgggtttaa gcagcgttgc acataacgca
     7141 ggggtaagaa tcaatgtcac cagaacagaa agtgccattg ccgaaacgat ggtgatagag
     7201 aactggcgat aaattgcccc agtagaaccg ccgaagaatg ccatcggaat aaataccgct
     7261 gacagcacca tcgcgatacc caccagtgcg ccctgaattt gcgacatcga tttttccgtc
     7321 gcttcttttg gcgggagctt atcctccatc atcacgcgct cgacgttctc caccaccact
     7381 atcgcatcat cgacgagcag cccgatggca agcaccatcc cgaacatcgt tagtgtgttg
     7441 atggagtaac caaaagcggc gaggatggca aacgtcccta acaacaccac gggtaccgca
     7501 atggtgggga tcagcgttgc tcgcatattc tgcaagaaca gatacatcac caggaacacc
     7561 agcataatgg cttcgaacag cgtttttacc acttcgtgaa tagaaagctg gacgaatggc
     7621 gtggtgtcat aagggtagag aaccttcatt ccctgcggga agaatggctg taattccgcc
     7681 agttttgcct taatggcttt cgcggtatcg agagcattcg cgccggttgc cagcttaatc
     7741 cccaggcccg ccgccggttt tccgttgata cgagcgataa cgttatagtt ttcaccgcca
     7801 agttcaaccc gtgcgacatc tttcaggcgt accaccgagc cgtcactgtt tacgcgcagg
     7861 gtcactttgc cgaattcttc cggatttttt aaccgcgttt gagcaataat cgaggcattc
     7921 aactgttgcc ctggtaacgc tggcgttccg cccaactgtc cggcagcgat ctgatcgttc
     7981 tgtaccttca actggttaat cacatcaacc ggtgtcagtt tatatttgtt tagcagatcg
     8041 gcgtccagcc agatacgcat cgcatactgt gcgccgaaaa gctgtacgtc accgacgcca
     8101 ttcagacggc taagcgtatc tttaacgtta gaggccacat agtccgagat atcgtcctgt
     8161 gtggtgcctg ggttatcaga gacaaaaccc gccaccatca aatagctgct actggacttt
     8221 tcaacactga tcccctgctg ctgaacctcc tgcggcagca acggcgtggc gagctggagt
     8281 ttgttctgca cctgcacttg cgcgatatca ggatcggtcc cggactggaa ggtaagggta
     8341 attgtcacgc taccggcgga atcgctggtg gaggacatat acatcaggtt atcgatacca
     8401 ttcatattct gttcgataac ctgcgtcacc gtatcctgca cggtctgcgc atcagcacct
     8461 ggatagtttg ccgaaacaga aaccgcaggc ggtgcaatgg ttggatactg agcgacgggc
     8521 aattgtagga tcgccagtgc gcccgccatc atcagaataa tggccagcac ccatgcaaat
     8581 atcggtcgtc gaataaaaaa gtttgccatg tcagattacc ttacttcgat gcagtatctg
     8641 cgggggtatc ggtagtggct ttaacctgct ctcccggacg cgctttttgc aggccgctga
     8701 caatgacttg atcgccagat ttcagacctt cgctaatcaa ccatttatcg ccaatcgcct
     8761 gactggcaac gacagggcgc gcttccacct gacttttatc gttaacaatc agcacggttg
     8821 catcaccacg cggtgtgcgg ctaacgcctt gttgcgggat aagaatggcg tcaggttgga
     8881 cgccttcatc aatccgtgca cgcacataca tacccggcaa aagcgtatgt tgcgggttag
     8941 ggaagacagc acgtagggtt atggagccgg tgctttcatc aacggtcaca tcagagaatt
     9001 gcagcgtacc tttcagggga taggtttgac cgttttccat gaccaactct acgttgctgg
     9061 tggcgttttc cttatgcaaa tttccttgct ctacggattg cttcagcctc ataaaatcgt
     9121 tgctggattg ggtcacatca acgtagatag gatcgagctg ctggacagtc gccagttcag
     9181 tcgtttgccc attagtgaca agagcgcctt cggtcacggt cgatttgccg atacgtccgc
     9241 taattggcgc agtcactttg gtataagcga gattgatgcg agcgctttcg actgtggctt
     9301 ttgcggcaat cacggcggca tcggcctggc gagcatcagc aatggcctgg tcgtactcct
     9361 gctggctgat gtatttcgta cccacgagcg gaacgtaacg ttttaccgtc aaatgcgcga
     9421 tggcggcggc ggcttcactt ttcgccagtt cgcctttcgc gctgtcataa tttgcctgat
     9481 aggtcgcggg atcgatctgg tacagggact ggcctgcttg tacatcgctg ccttcagtga
     9541 aattacgatt cagtacgatc ccgctaacct gtgggcgaac ttcggctata cggtaagcat
     9601 tggtgcggcc tggtaattca gtcttaactt ctaacggggc cgttttcaca atatgaacgg
     9661 taacctgcgg ttcaccgacg tgagctttct cttctccctt atcgttacaa ccggcgatta
     9721 aagccgcgga gatcagaata aaggagggca ggaggaaaaa cctggcatgt ttcgtcatta
     9781 ctattcctca aaaaaccaaa agcgcgttat ttacccagaa aggcaacgcg ttaactcgca
     9841 gaaagaaaaa tacagtttgc tatcctacaa attatcattc gtcgatgtaa ggaatagtta
     9901 tgaatactgg cgtctcaagg cacataaaca caaaaaaaga ttaatattct actgttttat
     9961 tttgacgcgg gttgaaagag gcagaattaa aacctcgtaa attgaaatat atattcatgt
    10021 ggcgaatgta ttttaggtaa ataatataca ttgtttattt gacgatttat agcaactcaa
    10081 tgttaccctc tcgtataaat acacattaga tgatagatta accttcgcta ttttctcacc
    10141 ctgtgtcgaa tatatttatt tcctgaataa ttaatcatgg caaaaagaac caaagccgaa
    10201 gctctgaaga cccggcaaga actgattgaa actgccatcg cccagtttgc gcagcatggc
    10261 gtaagcaaga cgacgctcaa cgacattgcc gacgccgcta acgttacgcg tggcgctatc
    10321 gactggcact tcgaaaacaa gactcaactg tttaatgaga tgtggttgca acagccttca
    10381 ttgcgggagt taatccagga acacttgacg gctggattag agcatgaccc gtttcaacaa
    10441 ttgcgtgaaa aattgattgt cggcttgcaa tatattgcca aaattccccg ccagcaggcg
    10501 ttgctgaaaa tcttatatca caaatgtgaa tttaatgatg agatgctggc cgagggagtg
    10561 atacgcgaaa agatgggctt taatccgcag actctccgcg aagtattgca ggcgtgtcag
    10621 caacaaggtt gtgtagcaaa taacctcgat ttagatgttg tgatgattat tattaatggt
    10681 gccttcagcg gaattgttca aaactggtta atgaatatgg cgggttatga tctttataaa
    10741 caagcacccg cgctggtcga taacgtatta agaatgttca tgccagatga aaacataacg
    10801 aaattaattc atcaaacgaa tgaattaagt gtcatgtaaa ttcacattct gtcctgaaaa
    10861 ttcagttcgt cacagttaac cagttttagt ggattaactg agttcatatt acggcatttg
    10921 tctgtttctg ttgtcagcag ttcaatccac tgcatcagta aagtatcaaa cagaaaaacg
    10981 aagacagcga aaacgacgag ccaccaatac ttgcgaatca ttacctgagc ctgtgcatgt
    11041 ttaaccaagt ctggcaggaa atatacacga aatgaataat ctgaaaacca ggcttacact
    11101 tcctttactt tgcaatgaga tcggggtcaa cttcgcttaa ccggcttcgt tttgacaggt
    11161 tgccggtctt tctttttttc actttcgcca gttcttccgc agagtaatgc gacgcgacat
    11221 ccagccgtcg aagccccatt ttgatgtact cgctgttgat ctcaatacca atgaattttc
    11281 gcccgctgga gacggctacg gcaccggtag taaagctacc ggcaaacggg tcgagaacga
    11341 tatcgcctgg gttggaagag gcgagaataa tgcgtttcag taaggcttcc ggtttttgcg
    11401 tcgggtggtt ctcatattca tccatcaaat aacgcacgcg cggaaaatcc cagacgttac
    11461 ccggtacttt ttgatgattg tatggctgtg gaggattttt gcgataatcg atcaacgcgc
    11521 gctgcgatcc ggttttggct tctaccagaa tagcatcacc gttgaatgtg tagttctttg
    11581 cgtctttcac catcatcagg atggggtcgt acatggagcc gtagtgtttt ttcgcctgta
    11641 caccagaact gtcatatgac cagacgatgc gacttttgat ggtaaaaagc ttgcggcact
    11701 ggagatcgat aaagggcatg ttttccgtgc tgttcatgat gtacatgctg ccctgctttt
    11761 tcagaacgcg gtggcactct gcaatcactt caaacagcca gtcgataaac agatcttctt
    11821 tccaggcgtc gatcagacca tcaaaatttt taccgatgtt atatggtggg tcggcaagga
    11881 tcagatcgac actttcggcg gggatctttt taagttcggc aagcgcatca ccgtgaataa
    11941 tggtcttagc ttcattacca aaccgggtcg gttcacatcc tgttctcatg gtcactccct
    12001 ttgtgacacc tataaaaaag gcgcttcccc atgccgagta gcgccttttt aatcaagcat
    12061 ttagctaacc tgaattagtt catgccgtat tttttcaatt ttttacgcag cgtaccacgg
    12121 ttgatgccca tcatcagcgc agcacgggtc tggttaccac gggtgtattg catcaccatg
    12181 tccaacaggg gctgttctac ttcagccagt accagctcat agaggtcatt cacatcctga
    12241 ccattcagtt gagcaaaata gttcttcagt gcctgtttaa ccgagtcacg caggggtttt
    12301 tgggttacct gatcctgaga gttaacggta gaaacggtca gtacgtcaga atttacgcgt
    12361 tgttcgaaca tagttctgtc agctctttat ttctgtttac gcaaaatttt cgaagtatgc
    12421 ctccaacgcc tccagctgtt cgctagcatc ctcaatggcg ttgaatgtgc gccgaaactg
    12481 gtcatttgga gcgtgttcct ggagatacca ggaaacgtgt ttacgtgcaa ttcggtaccc
    12541 ttttgccgga ccataaaagt catgcagttc ccgaacgtgc gcgcaaagca agcgcttaac
    12601 ctctgccaaa ggcagtgggg gcagcaactc cccagtgtcc agataatgct ggatttcccg
    12661 aaagatccag ggtcttccct gagctgcgcg gcctatcatc agggcatccg cccctgtata
    12721 gtcgagcaca gctctggctt taagcgggtc agtaatgtcg ccattcgcga taaccggaat
    12781 ggaaactttc tgcttaactg cccgaatact gtcgtactca gcttctccat tgaacaaaca
    12841 ggcgcgtgta cggccatgaa tggtcagagc ctgaatgcca cagtcttcag ccagttgggc
    12901 aatctcttcg cagttacggt gttccggtgc ccagccagtg cgaatcttca gggtaacagg
    12961 aacgtccact gcattgacga cctcggtaag gatcgattta acgacatccg ggtactgcaa
    13021 gagggctgaa cctgcgagct tgcgattcac ttttttagcc gggcaaccca tattgatatc
    13081 aataatctgg gcaccgcttt ccacgttaat acgtgctgca tctgccattt ctttcggatc
    13141 gctaccagca atttgcacgg tgcgaatacc gggttcatca atgtgcacca tccgtaaacg
    13201 agatttgtcg ctttcccaaa cctgtgggtt agaagacatc atctcggata ctgtcaatcc
    13261 ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct gtaatgccag ccatgggcgc
    13321 tgcgatcagg cgatttctga gctgatattg tccgatgcgc atgagttaag aaatgaccat
    13381 actgtgactg caaggcggcg tatattacgc attttttgca cgagatgaaa ggccaaactt
    13441 tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa atgagcgtgg tgcgataatt
    13501 acttataaaa atcatcatat tagaaaatag tgactaaaat ttacattcaa agaaatttga
    13561 gtaagttctc aatttttctt tatgaatgaa aattttggca cgcaaatttg cgtaaataac
    13621 cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc gccccgcgtc accctacggc
    13681 gatgcgaagg ttaattctta cgaccggtaa tacggcacca ctcttctttt tccacgaccg
    13741 ggtccagtgc gaagctatcg gcataagctt cacaaacgct ctctgcctgg cttgccagaa
    13801 taccggaaag gcccagcaaa ccgcctgaaa ccggcaggac gctgattaac ggtgccagtt
    13861 cacgtaatgg gcctgcaagg atgttcgcga ccaccacgtc ggctttcatt tcttctggct
    13921 gatctttcgg taagtagagt tccagacggt cagaaacgcc attacgttcg gcgttgtcgc
    13981 ggctagcctg aatcgcctgc ggatcgatat caataccaat ggcttttgct gcacccagtt
    14041 tcagcgccgc gatagccaga atgccggaac cgcagccaaa gtcgatgact gttttaccag
    14101 ttaaatcgag gctgtcgagc cattgtaggc acagagaggt agttggatgg gtacccgtac
    14161 caaacgccag ccctgggtct aacatcacgt tgacggcgtt ttcgtccggc acatcacgcc
    14221 agcttgggca gatccacagt cgttcaccaa agcgcatcgg gtggaaatta tccatccatt
    14281 cgcgctccca gtctttatct tccagttgtt cgattttatg cgcgaagcct gcgccgagca
    14341 gcggatggtt ttccagaatc gccaccacgt cgttcatatc ggtttcagcg tcgaacagac
    14401 caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg cagcggctca aatactggcg
    14461 tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc ttccatcagc gcatcgctaa
    14521 gatcttcagc gttcgcgccg gtggtgttca gtttcagttg gatccaaggc atggcaaaac
    14581 tctttattta tcagtagtca aaacggtagc ttgcgggacg gatgtaccga aacggtttcc
    14641 gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg atagggtgga agcccaggta
    14701 ctgaatattc agcgtcgcga gtacggcata cagcacgccg ccaacgatca tcgcacttag
    14761 cgcgcctttg gcgttggcgc gttcccagta aagacccagc accagcggcc acaggaaaac
    14821 ggcttccaac ccaccgaagg ccagcaaatt cagccagatg atcatttctg gcggcttcca
    14881 ggcggcaagc agcagcaacg cgccgagaac taacgtaatt accgccgaca tccgcttcag
    14941 acgcgtctcg ttttgcattt gatccggacg gatattcaga tagagatctt taatgatcgt
    15001 agcggaactt tgtagcaatt gggcgttaat tgtcgacatg atcgcagcca taggtgcagc
    15061 cagaaagatc ccggcagcaa acggtggcag cacttttacc attaacgtcg ggatcaccag
    15121 gtccggtacg gtgagatcgg ggatcactgc acgacctaac gctccggcca ggtgcatacc
    15181 gaacatcagg attgcgacta caatcgtacc gatgatgatc ccccggtgta ccgctttgct
    15241 gtctttataa gagatacagc gcaccgcagt atgcggcagg ccaatcacgc caaagcacac
    15301 cagtacccag aacgacgtca taaaggcagg cgacagaata tcgtcagcgc cttgtggcgt
    15361 aaccagttgc ggatcgatgg tttgcagcgt ctctaccgca ttacttaaac cacctgcggc
    15421 atgtaccacg ccaataagca gcacaacggt gccaatcagc atcacaagcc cttgcatggt
    15481 gtcgttcagc acgctggcgc gaaagccgcc aaaggcggta tacaacgcaa tgctgatacc
    15541 gaaaatcagc agcccggttt cataaggaat acccgccgcg gtttccagca ggcgcgcacc
    15601 gccgataaac tgcacggtca ttgccccaac gaacgcaacc agcaaactca aactcgccag
    15661 ccacaccaga agacgactct ggtagcgggc aaacagcatg tcgttcagcg tcactgcatt
    15721 gtagcggcgc gcaagaatcg cgaacttctt gccgagaata ccgagcgaaa gccagactgc
    15781 aggaagctga atcatcgcca gcaataccca acccaggccg tatttataag cagctccagg
    15841 cccgccgata aacgaacttg cactgatata ggtcgcggtg agcgtcatcg ccagcacaat
    15901 accgcccata gagcggctgc cgaggaaata ctcattaagg aaggtaccgg tgctccgttt
    15961 acgcatcgca taaacagaga taccgaacac caccaccaga taggcgacca gcggtagaat
    16021 cacttcaagc tgcatcgtca tcctccagtg ggatatcgcg atagataaat ttcaccatcg
    16081 cccagcacag tccaataaac agcagcggcg tcaggatgca ggccatctca aaccagcgcg
    16141 gaaagccggt aaaaccgggg gcaacgtcag gtaagtaagc ggctgctaac caaactgcca
    16201 gatacaaaag ggtcagcccc agcgcccagc gcgcctcttt atgggcctga acaaaacgag
    16261 tgtccacgga agggttcctc ctcatggggg gatacgagaa acgggcaatt ctacggcaac
    16321 tcttcaggca gggaaaataa tctgacagaa aattgccgtt cagtgctgct gcaaaaagtc
    16381 ttcaatgtca gccagtgagg atatcccttc catcggacct tgccgcatta cggcgagcgc
    16441 gccgctggca tttgcatatt gcagtgcctt atgtgccggg aaaccggata agaaaagagt
    16501 gataaacgtt gcgccaaagc agtcgcctgc acccgttgga tcgataattt cgatatcgtg
    16561 gcctgtaaca tgctgggcgt gtaatgtacc gtttttaagc ttgtaatagc tggccccacg
    16621 ctgggcgcgt tttatcgcca catgttttac gccgccgtgg agaagatcgc taacaatctg
    16681 ttcttctgac agatttttgt gacgcgcgaa gaaagggagt tcgctttcgc tggggatgaa
    16741 aatatccgta tattcaatca aataatcgag agcctgcgcc atttcaggaa tgctcagcat
    16801 ctctttgcga atattgggat cgaaagaaac ggtgccgcca gccgatttga tcgtcgttat
    16861 tgctttacgc atgacatcga tcatgcgaaa tgagaacaac gatgagccca caatatggag
    16921 atggttacac tgttttagca gatccttatc aatgtgctca gcagtaaaca agccgcaggc
    16981 gctgttaggg atattaaaga cgaaatcgcg ctgctgggga ttttgataac tcacgaacgc
    17041 cgtacctgtg acggcattgt tcataacatg gatcccatcg gtaatgacac cttcatgacg
    17101 gaggcgctct ataatgagtc ggccaaaatc atcattacca acacatccga agaagatggc
    17161 ccgatgggac agttttgcca cctgagcagc aaaaattgct ggcgcaccgc tgggaaatgg
    17221 cccgataaac tccccaggct gagtgaatgt ttgatgtggg ttgcgggaca aaaactcggc
    17281 cagcaattcg cccatagcgc agatagttat tgtcacaaaa cccctctcat tcctgaagcc
    17341 tttcgcgatt ggaccaggta aagcactcat tactggtata aaggataacg aaatcagccg
    17401 actgcaattg ttgctgatat tgttcgttaa tgctgcggct gacgatcaca ttttctgcct
    17461 gatgcaaatg cccgatagca gccgttgatg gcgcaggatc ggcggcaacg ataaccgtct
    17521 tattctgggc atgatcgaaa cagagctgcc aggcagcggc cagtctggga acgttgatat
    17581 ttaaagtacc atcagcatcc agatggttga tttccagaat gctatgtgta atggtgaaat
    17641 gctgaagtgc ctgttcaagc ggttttccgg ccagtgcggt gtcggggcgc agcatttcac
    17701 tgccagccag tattatttcg ccatcaataa actgatttgc cagcagcgca tgttcgggat
    17761 cgttggtgat cagacgcagt tttttcactt cgctgagcaa tggaatgatc ttacggcaga
    17821 ttgtcccatg tccaagaaat agcgtgtcac gatcgcgaat tagccgtgcg caatgtcgtg
    17881 ctatttccat ttgttgtgcc agagacaatg gcgcgatcgg ttgtatcgct tctggttcag
    17941 aggagagtgg tgtcgcaata gcaccgccaa atgatcgctt taaatatccc tgttgctcca
    18001 gataactgag atcgccgcgg atcgtcacgc cagagacacc aagttgtgca gataagtcat
    18061 caactttgat ttcaccttgg gcggcaacaa tttctgcaat ttttaatctg cgtttcagta
    18121 ggttagacga ttttctcggc acgttaacac tcttcattgg cctttaatgc gtaacactat
    18181 agccccttgt atttttcttt gaaagttatc cttcaataat atcgacaatt cgaaaagaaa
    18241 atcatgggtc aaaaatcgca acaaaaatag attaattaat tgaaaaataa atgaatttat
    18301 tcatatttat gcagagatgt tattggtttc acttgtaaag tgaaaaacaa attaagttat
    18361 ttttttgtaa gttattttga tgatattcga catgtatcac gctttttgcc gcttttgctg
    18421 tgctagtttg ctttcaaaat gaaagttaat attctctttc ttcattttgt aaggcgaacg
    18481 acaaataaca ggagctgcga tgtacatcat ttcaagcaaa aacatgctga aaaaagccca
    18541 gtcagaggga tatgccgtcc ccgctttcaa cattcataac cttgaaactt tacaggtagt
    18601 tgttgagagc gctgcacaac ttcgctctcc cgttatgctg gcgggaacac ccggtactta
    18661 tcgttatggt ggcgtgggaa gtttaatttc gttagtacaa tcgctggctc gcgagtacaa
    18721 cctgccgtta gtcctgcatc tcgaccatca tgaagagagt gatgacattt ttaacaaggt
    18781 tcgcgcaggg atccgttctg tcatgattga tggctcccat ttgccatttg aagacaacat
    18841 tgcgcgagtg caagaagtta ccaccttttg ccatcgcttt gatgttagcg ttgaggccga
    18901 gctagggcgt cttggcggcc aggaggacga tctgctggtg gatgaaaaag attccgctta
    18961 tacctcacca gccgcagctg cagagtttgt tcaacgtacc caaattgact ccctggccgt
    19021 agcaatcggc acagcacatg gtttatatgc gcatgaacca cgactggatt ttgtgcggct
    19081 ggaacagatt cgtcagcgag tggagattcc actggtgttg catggtgctt cgggcttatc
    19141 agaacaggac gttcagcact gtattcgccg cggtatttgt aaagtgaatg tcgcgacaga
    19201 attgaaaatt gcgtttgccg atgcgttaaa aggatatttc tacaacaatc ctgcggcaaa
    19261 tgatccacgc cactatatgc agtccgcaaa ggccgccatg aaagaagtcg taactcgtat
    19321 tattggtgtc tgtggtagtg aaggaaaaat ttaagacgtt gtaccctaca aaaacaatga
    19381 ggattgttta tgaaaaatat taaattactt ggttcagcta tgttggtgag cctggcattg
    19441 ttctcgcaaa attcgttggc aaaagagtat aaaattggtg cgtcattatt aacacagcaa
    19501 catcctttct atattgatct tgcgaatgcc atgaaagaag aggcaaaaaa gacaatgtca
    19561 atttaagtgt ct
//
LOCUS       DAJTMK010000069        19543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTMK010000069 DAJTMK010000000
VERSION     DAJTMK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19543
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.070"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(8..67)
                     /gene="ibsA"
                     /locus_tag="OO135_002091"
     CDS             complement(8..67)
                     /gene="ibsA"
                     /locus_tag="OO135_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR5397932.1"
                     /translation="MRKHVIILVILLVISFQAY"
     gene            complement(339..395)
                     /locus_tag="OO135_002092"
     CDS             complement(339..395)
                     /locus_tag="OO135_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5397933.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            complement(667..723)
                     /locus_tag="OO135_002093"
     CDS             complement(667..723)
                     /locus_tag="OO135_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5397934.1"
                     /translation="MMKILIIVVLLVISFPAY"
     gene            1002..2249
                     /gene="mdtA"
                     /locus_tag="OO135_002094"
     CDS             1002..2249
                     /gene="mdtA"
                     /locus_tag="OO135_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5397935.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELEAQQTLVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPSAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            2249..5371
                     /gene="mdtB"
                     /locus_tag="OO135_002095"
     CDS             2249..5371
                     /gene="mdtB"
                     /locus_tag="OO135_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5397936.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVNDTQFELMMAIALVVMIIYLFLRNIPATIIPGFAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5372..8449
                     /gene="mdtC"
                     /locus_tag="OO135_002096"
     CDS             5372..8449
                     /gene="mdtC"
                     /locus_tag="OO135_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5397937.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQATINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8450..9865
                     /locus_tag="OO135_002097"
     CDS             8450..9865
                     /locus_tag="OO135_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5397938.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASCHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFITPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9862..11265
                     /gene="baeS"
                     /locus_tag="OO135_002098"
     CDS             9862..11265
                     /gene="baeS"
                     /locus_tag="OO135_002098"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5397939.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLSSMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVISSPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            11262..11984
                     /gene="baeR"
                     /locus_tag="OO135_002099"
     CDS             11262..11984
                     /gene="baeR"
                     /locus_tag="OO135_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5397940.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            12175..12507
                     /locus_tag="OO135_002100"
     CDS             12175..12507
                     /locus_tag="OO135_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5397941.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12654..14015
                     /gene="yegQ"
                     /locus_tag="OO135_002101"
     CDS             12654..14015
                     /gene="yegQ"
                     /locus_tag="OO135_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5397942.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(14346..14663)
                     /locus_tag="OO135_002102"
     CDS             complement(14346..14663)
                     /locus_tag="OO135_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397943.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            15069..15968
                     /gene="yegS"
                     /locus_tag="OO135_002103"
     CDS             15069..15968
                     /gene="yegS"
                     /locus_tag="OO135_002103"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5397944.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEVLDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVISIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(16050..16823)
                     /locus_tag="OO135_002104"
     CDS             complement(16050..16823)
                     /locus_tag="OO135_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5397945.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSDVDKLALQKTRVKLITV"
     gene            complement(16929..17969)
                     /gene="gatD"
                     /locus_tag="OO135_002105"
     CDS             complement(16929..17969)
                     /gene="gatD"
                     /locus_tag="OO135_002105"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5397946.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVLLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(18017..19372)
                     /locus_tag="OO135_002106"
     CDS             complement(18017..19372)
                     /locus_tag="OO135_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5397947.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(19376..>19543)
                     /gene="gatB"
                     /locus_tag="OO135_002107"
     CDS             complement(19376..>19543)
                     /gene="gatB"
                     /locus_tag="OO135_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5397948.1"
                     /translation="RVNEIETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGIGIEA
                     LQNKILTILQG"
BASE COUNT         4592 a         5234 c         5045 g         4672 t
ORIGIN      
        1 ggtgttctta gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct
       61 tcctcataac cctttcctta tttaaggccc cttcctcggg aggggctttc ccgtttcagc
      121 atcccgctga aatcgtcggc ttacctcctt tcgccatgca tgcagtctat cgctgacttg
      181 cggataaaat agtttccagt gtgattattg cgtaacgtct cgcaaagaat atttgctggc
      241 aggatcgcag actgcaaagc ctgcagattg acaatctgac cgtgaaggca tactttcagg
      301 agtgagggtt gagcggggtt tcccccgccc tggtagtctt agtaagctgg ccagctaatg
      361 actaagagca ccacgatgat gagtagcttc atcatgaccc tttccttatt taaggcccct
      421 tcctcgggag gggctttccc gtttcagcat cccgctgaaa tcgtcggctt acctcctttc
      481 gccctgaata cagtctatcg ctaacgcgtg aacaaaatag tttcctctgt gattattgcg
      541 taacgactcg caaagaatat ttgctgccag gatcgcagac tgcaaagcct gcggattgac
      601 aatcttatcg tgaaggcata ctttcaggag tgagggtaga gcggggtttc ccccgccctg
      661 gtagtcttag taagccggga agctaatgac taagagcacc acgatgatga gtatcttcat
      721 catgaccctt tccttattta agaccccttc ctcgggaggg gctttcccgt ttcagcatcc
      781 cgctgaaatc gtcggcttac ctcctttcgc caagcatgca gtctatcgct aacgcgtaga
      841 taaaatagtt tcctgtgtta ttactggatg cgtgctcgca aatgtgcccg ccattcagac
      901 gattcccgat agtgtttcat aattcctcca tttttctccc ttattggctg gctacactag
      961 tatcattccg cgaaacgttt caggaagaga aactcttaac gatgaaaggc agttataaat
     1021 cccgttgggt aatcgtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc
     1081 aaggccgcaa tgactcccgg agtgcagccc caggggcgac gaaacaagcg cagcaatcgc
     1141 cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg
     1201 ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattacc gccgctaata
     1261 ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc
     1321 agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat
     1381 tagcacaagc ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg
     1441 acctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt atcccgtcag gagctggagg
     1501 cccaacagac gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg
     1561 ccagcgcgca gctgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg
     1621 gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc gggatcgtgg
     1681 tgatcaccca gacgcatcct atcgatttgc tctttaccct gccggaaagc gatatcgcta
     1741 ccgtcgtgca ggcgcagaaa gccggaaaac cgctggtggt agaagcctgg gatcgcacca
     1801 actcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa
     1861 ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc
     1921 agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccatcag
     1981 ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagt gaaaacaagg
     2041 tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag
     2101 gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga
     2161 aagtggaagt ggtggaagcc cagagcgcca ccactccaga agagaaagcc accagccgcg
     2221 aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg
     2281 cccgtctcgc ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt
     2341 actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta
     2401 tccgaccatt caggtagtca cgctttaccc aggtgcaagc ccggatgtca tgacctctgc
     2461 cgttaccgcg ccgctcgagc gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc
     2521 gcaaagttcc ggcggtgcgt cagttatcac cttacagttc cagctaacat taccgctcga
     2581 tgtcgccgag caggaagtgc aggccgcgat taacgccgcc actaacctgc taccgagcga
     2641 tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct
     2701 cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt
     2761 cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg gcggtcagcg
     2821 tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga
     2881 aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg
     2941 cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg aagagtatcg
     3001 ccagctaatc atcgcctacc agaacggcgc gccgattcgt cttggcgatg tcgcaaccgt
     3061 agagcaaggt gcagaaaaca gctggcttgg cgcatgggcg aacaaagaac aggccattgt
     3121 gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca gcatccggca
     3181 gatgctgcca cagctcaccg agagtctgcc gaaatcggtg aaggtaacag tgctttccga
     3241 tcgcaccacc aatatccgcg catcggtcaa cgatacccag tttgaattga tgatggctat
     3301 cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc
     3361 cggcttcgca gtgccgctgt cgttaatcgg caccttcgcg gtcatggtgt ttctcgattt
     3421 ttcaatcaat aacctgacgc tgatggcgtt aactatcgcc accgggttcg tggtcgatga
     3481 cgccatcgtg gtgatcgaaa atatttcccg ctatatcgaa aaaggcgaaa aaccgttggc
     3541 ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcgct
     3601 gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga
     3661 atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac
     3721 accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc
     3781 ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa
     3841 agtactgaat catccgtggc tgaccttaag cgtggcactc agcacgctgc tgcttagcgt
     3901 gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaacg gcattattca
     3961 gggcactttg caggcaccgc aatccagctc ctttgccaat atggcccagc gacaacgcca
     4021 ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt
     4081 tgatggcact aacccgtcgc tgaacagtgc gcgtttgcag atcaacctca aaccgttgga
     4141 tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt
     4201 gccgggcgtc gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag
     4261 ccgcacccag taccagttta ccttgcaggc cacgtcactg gatgcgctca gtacctgggt
     4321 gccacagttg atggaaaaac tccagcagct gccacagcta tctgatgtct ccagcgactg
     4381 gcaggacaaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
     4441 gatcagcatg gcggacgtcg ataacgccct gtacaacgcg tttggtcagc ggctgatttc
     4501 cactatttat actcaggcca accagtatcg cgtggtgctg gagcacaaca ccgaaaatac
     4561 cccagggctt gcggcgctag ataccattcg cctgaccagc agcgacggcg gcgtggtgcc
     4621 gttaagctca attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga
     4681 tcagttcccg gtaacgacca tctcttttaa cgtgccagat aactattcgc tgggcgatgc
     4741 ggtgcaggcg attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
     4801 gttccagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt
     4861 cgcggcggtg gtggcgatgt atatagtgct cggcattctg tacgagagct ttattcaccc
     4921 gatcaccatt ctctcgacgc tacccacggc aggtgttggc gccctgctgg cgttaatgat
     4981 tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga tcggtatcgt
     5041 gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat
     5101 gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac
     5161 caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
     5221 actgcgtcgt ccgttaggta tcggcatggt cggcggcctg attgtcagcc aggtgctgac
     5281 gctgttcacc acgccggtga tttatctgct gtttgaccgc ctggcattgt ggaccaaaag
     5341 tcgctttgcc cgtcatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
     5401 cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggcttccg
     5461 tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
     5521 gctgcccggt gcgtcgccgg agacaatggc gtcttccgtt gccacgccgc tggagcgctc
     5581 acttgggcgc attgccgggg ttagtgaaat gacctccagc agttcgctcg gcagcacgcg
     5641 cattattttg cagtttgatt ttgaccggga tatcaacggc gcagcgcgtg atgtgcaggc
     5701 gacgatcaac gccgcacaaa gtttgctgcc cagtgggatg cccagccgtc cgacctatcg
     5761 caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc
     5821 gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga
     5881 cggtgttggt gatgtcgatg tcggaggcag ctcactgccc gccgtacgcg tcgggctgaa
     5941 tccgcaggcg ctgtttaatc agggcgtctc gctggacgac gtacgcactg ccatcagcaa
     6001 tgccaacgtg cgtaaaccgc agggggcgct ggaagatggc actcaccgct ggcagatcca
     6061 gaccaatgat gagctaaaaa ccgccgccga atatcagccg ctgattattc attacaacaa
     6121 cggcggcgcg gttcgtctgg gcgatgtggc gacagtgacc gactctgtac aggatgtacg
     6181 taacgccggg atgaccaatg ccaaaccggc tattttgctg atgatccgca agctgccgga
     6241 agccaatatt atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggaaac
     6301 cattccggcg gcgattgatc tgcaaattgc tcaggatcgc tcccccacca ttcgcgcctc
     6361 gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt
     6421 tttattcctg cgctcgggtc gcgcaactat tattcccgcc gttgcggtgc cggtttcgct
     6481 gattggtacg tttgcggcga tgtacctgtg cggtttcagt ctcaataatc tttcgttaat
     6541 ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat
     6601 tgcccgtcat ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga
     6661 agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct
     6721 gttgatgggc ggattgccgg gccgactgtt acgcgaattt gccgtgacgc tttctgtcgc
     6781 cattggtata tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct
     6841 gaaagccagc aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc
     6901 cctgcaacaa ggctacggca agtcactgaa atgggtgctc aatcataccc gtctggtggg
     6961 cgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac
     7021 cttcttcccg gagcaggaca ctggcgtgtt gatgggcggg attcaggcgg atcagagtat
     7081 ttcgtttcag gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc gtgacgatcc
     7141 ggcagtggat aatgtcaccg gctttacagg cggttcgcga gtgaacagcg ggatgatgtt
     7201 tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct
     7261 gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat
     7321 tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct
     7381 ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact
     7441 ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg
     7501 cgacaccatg gcacggctgg gaatcgacgt acaagccgcc aacagtctgt taaataacgc
     7561 cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat
     7621 ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa
     7681 taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaatgcccc
     7741 actatcggtg aatcatcagg gattatcggc ggcctcgacc atttcgttta acctgccgac
     7801 cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt
     7861 gccttcgacg gtgcgcggca gttttgccgg cacggcgcag gtgttccagg agacgatgaa
     7921 ctcgcaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct
     7981 ttacgagagt tacgtacatc cgctgacgat tctctccacc ctgccctcgg cgggcgttgg
     8041 agcgctgttg gcgctggagc tgttcaatgc cccgttcagc ctaatcgccc tgatagggat
     8101 catgctatta atcggcattg tgaagaaaaa cgccattatg atggtcgatt ttgcgcttga
     8161 agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg
     8221 ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggtgcgctgc cgctggtatt
     8281 gtcgggcggc gacggatcgg agttgcgcca acccctgggg atcaccattg tcggcggtct
     8341 ggtaatgagc cagctcctga cgctgtatac cacgccggtg gtgtatctct ttttcgaccg
     8401 tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagatct
     8461 tcccgacagc acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct
     8521 ggacaccacc atcgtaaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc
     8581 gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc
     8641 cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct
     8701 gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc
     8761 acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg gtcggcagat tgacggtgat
     8821 gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca
     8881 ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtgtca
     8941 ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgctgtt
     9001 aatgccgaac tacaccatgc agacgcggcg ctttgatctt tccggctttt tattgctggc
     9061 ggttggcatg gcggtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc
     9121 gctggcgatt gctggcctgg tcgcagttgg tgtggtggca ctggtgcttt atctgctgca
     9181 cgccagaaat aacaaccgcg ctcttttcag tctgaaactg ttccgcactc gtaccttttc
     9241 gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttattac
     9301 accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac tgatgatgat
     9361 cccgatggtg cttggcagca tgggaatgaa gcgaattgtt gtacaggtgg tgaatcgctt
     9421 tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctggtca ccctgttgtt
     9481 tatgactacc gctctgctgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg
     9541 gatggtcaac tcgacgcgtt tctcctccat gaacaccctg acgctgaaag atctcccgga
     9601 caatctggcg agcagcggaa acagcctgct gtcgatgatt atgcaattgt cgatgagtat
     9661 cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga
     9721 cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt ttatcatcgc
     9781 ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc
     9841 gcggcgaaaa aggagcgcgc aatgaagttc tggcgacctg gtattaccgg caaactgttt
     9901 ctggcgattt tcgccacctg cattgtcttg ctgagcagta tgcactgggc ggtgcgtatc
     9961 agttttgagc gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg
    10021 ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac
    10081 aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa
    10141 cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac
    10201 aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt
    10261 ctggtcaacg gtgcggaagt gggcgcggtg atctcctccc ccgttgagcg gttaacgcgc
    10321 aatactgata tcaatttcga taaacaacag cggcaaacca gctggttgat tgtcgctctg
    10381 gcaacgttac tcgcggcgct cgccaccttt ctgctggcgc gtggtctgtt ggccccggtc
    10441 aaacggctgg tggacggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg
    10501 cccaccagtg aagatgaact aggcaaactg gcgcaagact tcaaccagct cgccagcacg
    10561 ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt
    10621 acgccattag cggtgctgcg cggtgaactg gaagccattc aggatggcgt gcgtaaattc
    10681 acgccggaga cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac
    10741 gatctccatc agttgtcgat gtctgatgaa ggcgctctcg cctatcaaaa agcaccggta
    10801 gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagccgt
    10861 ggcctgaaac tgcaattttc cctgccagac agtatcaccg tatttggcga tcgcgaccgt
    10921 ttaatgcagt tattcaataa cttacttgaa aacagcctgc gctacactga cagcggcggc
    10981 agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagc
    11041 gcgccgggcg tcagtgacga tcagctacaa aaattgtttg aacgttttta tcgcaccgaa
    11101 ggttcccgga accgcgccag cggcggttcc gggctggggc tggcgatttg cctgaacatt
    11161 gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt
    11221 acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg
    11281 acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca
    11341 ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac
    11401 tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt ggatctgatg ctccctggca
    11461 ccgatggctt gacgctgtgc cgggaaattc gtcgtttttc tgacattcct atcgtgatgg
    11521 tgacagcaaa aatcgaagag atcgaccgcc tgctggggct ggagattggc gcagatgact
    11581 acatctgcaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc
    11641 gttgcaaacc gcagcgcgaa ttgcagcaac aggatgctga aagtccgttg attatcgacg
    11701 aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc
    11761 gtctgctgaa aacgctctct cacgaaccag gaaaagtgtt ctcccgcgag caattgctca
    11821 atcatcttta tgacgactac cgcgtagtaa ccgaccgcac catcgacagc cacattaaaa
    11881 acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttatg
    11941 gcgtcggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacattatt
    12001 ttgttagccg gagatggcgt tcagacccga tccggcaaca ttatcccacg catggtcagc
    12061 aaactgacat tatctctccg ccctggcagg catcagaaaa tcgctcatac tttaatcggt
    12121 aaacagcacc tttagatgct gttttcgata cactatttca atcaaggagt cattatggct
    12181 ggttggtttg aactcagtaa gagcagtgat aatcagttcc ggtttgtgct aaaagcgggc
    12241 aatggtgaga ctatcctcac cagcgagctg tatacctcaa aagcctctgc ggaaaagggc
    12301 atcgcgtcgg tgcgtagcaa cagcccgcaa gaagaacgct atgagaaaaa aacggcaagt
    12361 aacggcaaat tctatttcaa tctgaaagcc gctaatcatc aaattatcgg ctccagccag
    12421 atgtacgcca ccgcgcaatc tcgtgaaacc ggaattgcct ccgttaaagc caatggcaca
    12481 agccagacgg tgaaagacaa tacgtaatta cgatgccggg cgtgggttgt tggcgtccgg
    12541 cgctttacca gatcccccca cagcgctaca atgcccgccc ttaaagtggg ggcactcccc
    12601 taaccgcttc atcaggtgaa gcggatctga cctgtcatca gaacgagaga attatgttta
    12661 aaccggaact cctttccccg gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg
    12721 gcgcagatgc tgtttatgcg ggccagccgc gttactccct gcgtgtgcgc aacaacgaat
    12781 tcaaccacga aaatcttcag ctcggcatca atgaagccca cgcgctgggg aaaaagtttt
    12841 atgtcgtggt caacattgca ccgcacaacg ccaagctgaa aacctttatc cgtgacctga
    12901 aaccggtggt ggaaatgggg ccggatgcgc tgattatgtc cgatccaggg ctgattatgc
    12961 tggtgcgtga gcacttccct gaaatgccga tccacctctc ggtgcaggct aacgccgtaa
    13021 actgggcgac ggtgaaattc tggcagcaaa tgggcctgac ccgcgtgatc ctctctcgcg
    13081 agctgtcgct ggaagagatt gaagagatcc gcaatcaggt gccggatatg gagatcgaga
    13141 tcttcgttca cggtgcgctg tgcatggcct actccggtcg ctgcctgctc tctggctata
    13201 tcaacaagcg cgatccgaac cagggtacct gcaccaacgc ctgccgctgg gagtacaacg
    13261 tccaggaagg gaaagaagat gacgttggca acatcgtaca caagtacgag ccgattccgg
    13321 tgcaaaatgt tgagccgacg ctgggtatcg gcgcgccaac cgacaaagtg tttatgatcg
    13381 aagaagccca gcgtccgggc gagtatatga ccgcgtttga agatgagcac ggcacttaca
    13441 tcatgaactc gaaagatctg cgcgccatcg cccatgtaga acgcctgacc aaaatgggcg
    13501 tgcattcgct gaaaatcgaa ggccgtacta aatctttcta ctattgcgca cgcaccgcac
    13561 aggtttaccg taaagctatc gatgacgccg ctgcgggcaa accgttcgat accagcctgc
    13621 tggaaactct ggaaggtctg gcgcatcgtg gctataccga aggtttcctg cgtcgtcata
    13681 ctcacgacga ttatcagaac tacgaatacg gttattcagt ttctgaccgc cagcagtttg
    13741 ttggtgagtt taccggtgag cgcaaggggg acctcgcggc ggtagcggtg aaaaataaat
    13801 tctccgttgg cgacagcctt gagctgatga cgccgcaagg caacattaac tttacccttg
    13861 agcacatgga aaacgccaaa ggtgaagcga tgccggtcgc accaggcgat ggttatactg
    13921 tgtggctccc ggtcccgcag gatcttgagc tcaattacgc tctgctgatg cgtaatttct
    13981 ccggggaaac cacgcgtaac ccacacggta agtgattaat ttcgattatt tttcccgtat
    14041 ggaaaattct tagaaaccga tcacatacag ctgcatttat taaggttatc atccgtttcg
    14101 ctgaaaaaca taacccataa aatgctagct gtaccaggaa ccacctcctt agcctgtgta
    14161 atctccctta cacgggctta tttttttatg cataagccct atctctggta accgtcttcc
    14221 attgaccaca tcgatagaat cctccttcat agcacgatgc ctttcactta tcggcatcgt
    14281 gctcccacag gttccggcta cgcacagcca gaacgcgcat atttgacgct taccaaaaaa
    14341 tattctcact ctccacattt gaatgtcaga cgagcgacac catgtaatcc tacaccttct
    14401 gtcttcagct caactatttg catttttttg ccctgagtaa cacagaaatg agttgcatca
    14461 ttttttacta tattttctgc accagatatt ctacccctgg ctaaagaagc ttcggcttcg
    14521 gtgtagtatt ggttatcgag tttacgctga atattacttt tatatgcaag accaaattta
    14581 ccgatacttg tctcatcatt atgcacagca caaccagaca taataaaaat actaattaat
    14641 gatatagcag ctatcttttt catctcacct tcccccatta aataccaacg acactctcta
    14701 gtgtttaaat ataataatgg catgattatt ataattgaat aggattataa taaatgttct
    14761 gtacaacatt tcctacataa gtaggaatta cggacattga ggcccttcag ggtaactcca
    14821 tgggggcttt aatatattat attgaagatg ccactgttta gttgaatatt aggtatatgc
    14881 tcttttttga aatttatcgg tggcagccgt tagtattcgc tgtccccatt gcaagctcct
    14941 ggtggtaacc actgaatcct ccatacttga actgactttt tatcctccga ctttcatcct
    15001 gttctgactc caccttttgt tttctgctct acactatcta cagaccaatc ataaaggcac
    15061 atacgatcat ggcagaattt cccgccagct tactgattct taatggcaaa agtactgaca
    15121 atctaccctt gcgcgaagca attatgctgt tgcgtgagga aggaatgacg atccatgtgc
    15181 gggtcacctg ggagaaaggc gatgccgcac gatatgtaga ggaggcccgg aagttgggcg
    15241 tcgcaacggt gattgccggt ggtggcgatg gcaccattaa tgaagtttct acggcgttga
    15301 ttcagtgtga gggggatgac atacccgcgc tgggaatttt gccattagga accgccaatg
    15361 attttgccac cagtgtaggg attcctgagg tactggataa ggcgctgaaa ctggcaattg
    15421 ccggtaacgc cattgcgata gatatggcgc aggtcaacaa acaaacctgt tttattaata
    15481 tggcgacagg cggatttggg acgcgtatta ccacagaaac gccggaaaaa ttaaaagccg
    15541 cgctgggtgg cgtctcttac atcattcatg gcttaatgcg catggatact ctgcaaccgg
    15601 accgttgtga aatccgcggt gaaaactttc actggcaagg tgacgccctg gtcattagta
    15661 ttggtaacgg gcgtcaggcc ggtggcggtc agcaattgtg tccgaacgcg ttaattaacg
    15721 atggcttgct gcaactgcgc atttttaccg gcgatgaaat tcttccagct ctcgtatcaa
    15781 ccttaaaatc tgacgaagat aacccgaata ttatcgaagg cgcttcgtcg tggtttgata
    15841 tacaagcccc acatgaaatc acttttaatc ttgatggcga accgttgagc ggacaaaact
    15901 ttcatattga aatacttccg gcggcgttgc gttgtcgatt accaccggat tgtccattat
    15961 tgcgttaatc aaactattct ctcttgaacc agggagaatc cgtaatgccg gatgcggtgt
    16021 aaatacctta tccggcatac agaacaatac tacaccgtaa ttaatttcac ccgtgtcttc
    16081 tgcaatgcca gtttatccac atcagacaca gcatcatcgg taataataat gtcaattttt
    16141 tcgattggca gtacctgatt aaaaccgcga cggttaaatt tcgatgaatc gagaacggca
    16201 atcactttat tagcggctgt gaccatcgca ccgctaacgg aataaccctc attaaaggtc
    16261 gtaataccat taaccgcatc aataccatca gcaccgacaa acattaaatc ggcattaata
    16321 tcctgcaggg aacgttcagc aatagatcca tgcatagatc gcgttttatg gcggaccgtg
    16381 ccaccgcaga cgaccagagt aatgtcttta ttttcggaaa gggcaaacgc agccgggaga
    16441 ctgttggtaa tcaccgtgat atttttggcg gtcattaatc cttcggcgat aagcattgtg
    16501 gtacttccgc tgtcgagaat aacggtcatc ccttcatgga tcattttgac tgccgcctgc
    16561 gctattctgt ttttcggcgc gctggcgagc tgaaatcgct ctttaaaccc gacttcctgg
    16621 gtctcggttt cactattacc agacattatt ttcgccgcac cgccatgaaa gcgcgtaacc
    16681 acgccttttt gttcgagaaa gcgcaaatcg gcacggattg tcgcttccga ggcagcaaat
    16741 actcccgcca gatcctgaac aagcacggtt ccctgttcat tcactaattg gatgatctta
    16801 ttccttcgct cgaatgaatt catcgtcatt tacccatatt caattgtggc tagtgtaaac
    16861 gaagggggac cattggtgaa tgatctgttt tgcacaatac cgggccagca tcacgctggc
    16921 ccgcggtttc atggaatgag caacactttg cccggcatgg cattacgagc gatgtcacgc
    16981 accgcctggg tgaagctttc aaagcttcca cggtgagcga ttaatggctc caggcttaac
    17041 ttacgttctg tcagcaaccg gctcgccgtt tcccactcct gccccggcca agggctggaa
    17101 tagttcatcc aactgccgat aaccgtcagc tctttacgca atattttgcc aaacgttgcc
    17161 gatgttaaat gcagatcctg atgcaacgtg cccaccagcg ccagttgggc atgaggaccg
    17221 gcaatctcta ccgccagttc gacagtttgc ggtacgccag ccgtctcgag gataagctga
    17281 ttaaaacgca gttcgcgtaa aacgctctgc atttgcggcg cgctcatttc gctactgtta
    17341 aatgtttgca tcgcaccgaa agattttgcc agtgccagtt tttctgagct aatgtcgata
    17401 gccgtcacac tctttgctcc cagcgcgaca gcgcactgaa tagccagcag gccaatggtt
    17461 ccggcaccaa taataataac gtttttattc tcacaacctt gcgctaaatg aaaagcatgc
    17521 agaccaacgg taatcggctc aataaaagcc ccatcctcaa taggcatatc cgtgggtaga
    17581 gcaaagacat tttttcgctt aacgacaata tattcagcaa atccaccatc acgccgcgag
    17641 ccaataaaat catattttgc gcactgggaa taaaaccctt tcagacactc tggacaagta
    17701 aaacagggta ataacagcac acaggcaacc gcatcgccag ggtgtaaatc atcaacaccg
    17761 gatcccaccg catcaatata gccgctaaat tcatggccta acgttattgg ataataatgt
    17821 gcaccatttt taaatatcct gggtaaatcg gaaccacata agcccgagct ggcaattttt
    17881 acccgcacct catcctgatg tttaatttca ggaatgacgc tttctgcaac gcgcacgata
    17941 ccatcagtat cattcaccac tgatttcata aaaactcctg attgttaagg gggataacct
    18001 ccccctcagg taaaaattat tctgcgagaa cgactttctc ttgtttaata aagccacgcg
    18061 ctctacgcca ggtcatgaaa ataccggtca gataaattgc accgataata atgaaaccgg
    18121 gaatattttg cggggagaaa acctgaatca gtaaccaggt aatgggagaa ccgccctgat
    18181 ccattgaagc caccataccc ccggctttta acgccccagc attagccgcc agttgggtgt
    18241 gcaaaccaat agtttgcgtc gcgatccaca gagtgatgct catgatgatg acacccgaga
    18301 ttaaggtgcg gaacagattt ccacgatgca cggcgactgc catcgccaca aagaagccga
    18361 tggtggcaag atcgccaaac ggcagcacct gattacccgg aacacaaaca gcaattaaaa
    18421 tggtgagcgg gataaaaatc aggcttgccg ataccaccgc cgtatgcccc agcagtaatg
    18481 cgggatccag gccaatcagg aactcctgac cgccgaactt cgcctgtaaa cgactacggg
    18541 cctgcttagc gatgggcgtt aaaccatcca tgatgggttt aatcacccgt ggcattagca
    18601 gcatcactgc cgccgttttt accgccagct gcaatacacc tttgacatcg taacccgcga
    18661 ggatgccgat aatcagcccc atcacaaaac ccacggtgac aggctcgcca aatggaccaa
    18721 atttgcgctg aatatcgtcg gcgctaaatt taattcggtt aacgcctggg attttctcga
    18781 tgatagcatc gaccagcacc gcaatcggcc ccatatacgc cgacgtaccg tgcggaatag
    18841 caataccttc cagctcaaag aaatttcggg tatcgcgggc aaaccagtcg ccgagcttat
    18901 aaacaaacgc cgcgtgaatt accacacctg ccatccctat catccatgaa ccagttgcca
    18961 gatgcagcaa cgcgccggtg aaggtcatat gccagatatt ccagatatca atatttacca
    19021 cccgcgtcat acgggtcagt aacatcgcca cgttaaccag aatcgcaatc ggaatcgcca
    19081 ccagcgcaat ttgcgacgcc caggtcattg gtgaagagcc cggccagccg acatcgacca
    19141 catgcagatt caggtcgaaa ttttccgcca tcgctttcgc cgccggacca atggaatcca
    19201 gcattaagcc aatcacaagg ccaatgccaa caaagccaat cccgatatgc agacccgctt
    19261 taaagcaatc gcctgccttc atgcctaata ttttagaaaa aataatgatg acaatcggaa
    19321 gcatcaccgt agggccgagg tcgagaatat aacgcatgac ttctgaaaac ataggtcacc
    19381 cctgtaagat agtcagaatt ttattttgta atgcttcgat accgatacca gaaataaaag
    19441 gcatgccgtg aactaacgga atatcgccaa aactacgatc cactttggca gtggtgcata
    19501 tcaaatgcac accatccata taggtttcta tttcattaac ccg
//
LOCUS       DAJTMK010000070        19194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTMK010000070 DAJTMK010000000
VERSION     DAJTMK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19194
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.071"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            535..687
                     /locus_tag="OO135_002108"
     CDS             535..687
                     /locus_tag="OO135_002108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397949.1"
                     /translation="MVDNIDVMKNVEMLIGYRAIKSKYLSILQNFGAGNIHLDNANRV
                     WELRRI"
     gene            1188..2081
                     /gene="galF"
                     /locus_tag="OO135_002109"
     CDS             1188..2081
                     /gene="galF"
                     /locus_tag="OO135_002109"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5397950.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEALGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            2392..3711
                     /locus_tag="OO135_002110"
     CDS             2392..3711
                     /locus_tag="OO135_002110"
                     /inference="COORDINATES: protein motif:HMM:NF024832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HCR5397951.1"
                     /translation="MVIKNIFWQFAEKILRLIFAIFISALLARHLGVSGFGLYNYINA
                     IISVALVVTSLGFNRILVRESTCCKDKISHNELISTSFYSRLLFSILIFALLWGGYSY
                     IDNKNSIIYLISFLSVIFVSFDVIDYHLQGQCIFKTATLCRVVSFLIVSFIRIFLVFE
                     NATLIWFCITILFEYAISAILLYLSSRFIHRNIFTIKIKHVSLNKAKYLLKESWSEII
                     AGFGAILFMKMDQIMLQYMLNAESVGVYSAALRISESWYFLPAAIVSSVFPVIMNKYN
                     KENTNESALVELFGHIMTLLVWLSICAAITISLFGPAVIHWLFGDEYIRSATILIYHV
                     WAGVFLSMGILSGSWLVAKKKLKLNLYRNIGGLIVNFFLNIILIPVYSGVGAAIATIS
                     GFAFAFLFFDLFHKELRGMFFLKIKSLSPINLIQAIRFLSNIRLKYL"
     gene            3928..4186
                     /locus_tag="OO135_002111"
                     /pseudo
     CDS             3928..4186
                     /locus_tag="OO135_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            4460..6817
                     /locus_tag="OO135_002112"
     CDS             4460..6817
                     /locus_tag="OO135_002112"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015983.1,HMM:NF016145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HCR5397952.1"
                     /translation="MNMKNIIERIKPLFFDGSRHLVRPVVLQFPVIDICNSKCQMCRI
                     WENKKSVDISVEQLKKGIQNELFKDVMSIGFNGGEPTLREDLSEVIETCLYSLPKLKQ
                     ISLITNGFKYDQVIHQIEKIGKLIKSKDIFFDVMVSLDGFGVIHDKVRGRTGSFKHAQ
                     HVLTFLKNADYVSNIRIGCTVIKENVYYLHELLEFCIREKLYVKYRQGVPHKRLYTEN
                     LIEPYSLTFEEKYEFVEFLEGIIKYYEPSLLQKHFYRSLINQIIYEHPRTAGCDWRYR
                     GATITAKGELAYCAIESKTLMKDISDGNAYQIYFDNENHLNDIKENKCKNCHHDYVGL
                     PPPAEYRKIFLSSINEKWKIKDKIKKIPGGRGINKIINEYKYSTCINQYRNIHSTQVQ
                     DVSLRGNTRVLICGWYGTETLGDKAIIGGIINSVRSSLGYEVVFYIASLYPYITEMTR
                     RQMPELKNCQIVNIQQAILLAKDMTYVVFGGGPLMAIDDIAPMQVIFEKAKQAKVKTG
                     IMGCGIGPLGDKRYNNSIKTILELSDFRIYRDQKSKSEALRLGVNAENDIVAEDPAFS
                     WIKSYYEQSVVGGRHRKAYINDEKEILLLGLRDFPWREYAKHLSEKECLSIKDNFEEV
                     LVSALKEVVKKRDNLIIRPLPMCTNHFGSDDRWFYRRLFRDSGIPSENLDFSLCGREL
                     APTEYCKAFLDANALLAMRFHSLVFGLGLGTPSLALDYTLGKGKVKSLAERFGANVIS
                     LSKINKEELLLEIEDLLEHNSQESNVSILESIKFQQSVDTFMKRD"
     gene            6819..8027
                     /locus_tag="OO135_002113"
     CDS             6819..8027
                     /locus_tag="OO135_002113"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5397953.1"
                     /translation="MKILHICYSDCLGGAARAANRLHQAQRKLGINSFMLVVDKYTDD
                     DYVLELNKLTKFRIKILNMISGYILRHLIQKNPVKHSLNIFSAGVVNAINKLNPDIVN
                     IHWIGDNMLSIKEISKIKSPIIWTMHDMWAFSGCEHYDSFPGIRRYEDDYNKKDGIFD
                     LNRIIYRYKKKKWSHINMQFVSPSRWLAECACRTSLIEGKRVHVIQNTIDHVVFDKVD
                     KVCARKLLGLPQNRKLILFGAMSGTADHRKGFHLLNEALKYLSEKYATEYGLVIFGDK
                     KDGDDIHGFPTYRLGTFYDELSIKLIYSAADVYVAPSLQDNLPNTLVESLAVGTPCIA
                     FNIGGMPDLIINSKLGGLVTEVDSRALSIAILDVLNRNIDSNIIRNISLAERAEDNIA
                     HKYEDVYLNA"
     gene            8020..9213
                     /locus_tag="OO135_002114"
     CDS             8020..9213
                     /locus_tag="OO135_002114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397954.1"
                     /translation="MHKKLYYYFLIVSPLIYLTGGIEHVTPLSIAGYLDLPSLSLINY
                     IFFLWCVFFLHFYSFGKNILWLYLLDIVIIMIIFRESRVDSIFFSTLSQFLILSPLFL
                     LVGCKIHIGYERIKKISYIFVFYLICSILLHYIFIMYYKYIGFSLPNERAVGIFKNPN
                     HLAAFSILLLIFLYNLAFIKLVDVKKIFFAELIVAVTVYLSGSRSSQVFFIILLLIHS
                     FLYNKRFFSLYCCLAVLSVAYVGMSDIGVDKIEQLLTKRETKDIAEAGNMRMSILFDM
                     LENFNFREILIGKGSGQGTAVYITNQSNNNEKITWLDSNVNTLTYTYGVVFLIFSLAA
                     MLLKMLFLALMKRTGEFYIFFFVFYFMWFINIGEFFPLIFMLLIQVSTSNMGQQHDIN
                     NYYKL"
     gene            9185..9946
                     /locus_tag="OO135_002115"
     CDS             9185..9946
                     /locus_tag="OO135_002115"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5397955.1"
                     /translation="MISIITINYNNKNELYRTLSSIDSQKLKSHIEVIVIDGGSTDGS
                     QEIVSNSFKHLVTYFISEPDMGIYDAMNKGILLSNKEWLYFLNAGDIFFSDDVLNKLY
                     DFIINSNLTNLIYAPYISDGKIDNTQLFSLSYLSSHMINHQSMLLHKELFQKQLYDIN
                     YRFCADYALLLNLFDNIKPVKTDFIIVDYDSKGISSLDDNKVFIWKERILAVLKSNLS
                     FYKKLFLLKRAFGVYPYHLLKSKLKCMKNIMTHCR"
     gene            9913..10752
                     /locus_tag="OO135_002116"
     CDS             9913..10752
                     /locus_tag="OO135_002116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5397956.1"
                     /translation="MHEEYNDTLSIVFCALYDDWLKNKELIHRSFEHFVKIWEERCFF
                     ILIIQDAKQRKIELAFKNMHIFYMQERGISLARNRGVEEACTLNSRWIIFHDASICWT
                     EEAVGFLFKNRFSKISPKVKLKFQLIEEKPSTDVQGEIRKINPVYDTYVGSVLFEVQS
                     IAKIRFNIFHGPGQNTIYKSGEDVLFFFDYFSMKKNFCVYESKTAYIYHPPREKDFSK
                     HKIYAKGQGRVFKILLKEYFSIRLLMDCILFFGNALVRCIFFRKNSFYILKERCVGFF
                     CED"
     gene            10754..11872
                     /gene="gmd"
                     /locus_tag="OO135_002117"
     CDS             10754..11872
                     /gene="gmd"
                     /locus_tag="OO135_002117"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5397957.1"
                     /translation="MKKALITGITGQDGSYLAEFLLEKGYEVHGIKRRASSFNTDRVD
                     HIYQDRHNDSPNFLLHYGDLTDSSNLIRLIKEIQPDEVYNLGAQSHVAVSFESPEYTA
                     DVDAMGTLRLLEAIRICGLEKKTRFYQASTSELFGLVQETPQRETTPFYPRSPYAVAK
                     MYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANISQGIEKCLYLG
                     NMDSLRDWGHAKDYVRMQWMMLQQDHPEDFVIATGKQISVREFVRMSAREVGLELEFS
                     GEGVDEIATVVNKTSDCAIGVNIGDVIVRVDPRYFRPAEVETLLGDPSKAKKVLGWEP
                     EITVEEMCAEMVASDLAKAKQHALLKSHGYDVAVSLER"
     gene            11876..12841
                     /locus_tag="OO135_002118"
     CDS             11876..12841
                     /locus_tag="OO135_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5397958.1"
                     /translation="MTKKRIYVAGHRGMVGSAICRQLSLRDDIELVVKTHKELDLTVQ
                     KDVEVFFEQERIDQVYLAAAKVGGIYANNTFPAEFIYQNLMIESNIIHSAHKAGIQKL
                     LFLGSSCIYPKFAEQPMKESALLTGILEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGMNDNFHPENSHVIPALMRRFHEAKESGAPEVIVWGTGTPMREFLYVDDMA
                     AASVHVMELDDVIYQQNTQPMLSHINVGTGVDCSIREMAETMASVVGFQGKIVFDSTK
                     PDGTPRKLMDVTRLKNLGWQYRYNLHEGLSLTYKWFLANINSFRG"
     gene            12844..13305
                     /locus_tag="OO135_002119"
     CDS             12844..13305
                     /locus_tag="OO135_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5397959.1"
                     /translation="MKKRLEYELFKTIVEHTPLISIDLIIRNDKGEALLGQRLNRPAQ
                     NYWFVPGGRIFKDESFEAAFKRITFEELGVQTSLNEALFLGVYEHFYNDNFSETEFST
                     HYVVHGYEIQLNPQQLHLPTIQHNSYKWFDIETLLNSTDVHQYTKNYFISR"
     gene            13311..14717
                     /locus_tag="OO135_002120"
     CDS             13311..14717
                     /locus_tag="OO135_002120"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HCR5397960.1"
                     /translation="MLLPVVMAGGSGTRLWPLSRTLYPKQFLSLNSRLTMLQETLRRL
                     DKVEHKPTLVICNESHRFIVAEQLRKEGLKHSGILLEPVGRNTAPAVALAALQAKASG
                     EDPILLVLAADHEIQDEGRFIAAVLAAKNFAEQGKLVTFGIVPAAPETGYGYIKVGEE
                     LDGQGYKVAAFVEKPELDLAKQYLADGGYLWNSGMFMFRASIFLDELKKFRPDILASC
                     QQSLSSSTQDLDFIRLDNSSFSCCPEESIDYAVMEKTAEAVVVPLNAQWSDVGSWSAL
                     WEISSKDQSGNAIRGDVLIEDATDSYLYSQHRLIGAVGVKDLVVVETKDAVLVAHKDK
                     VQQVKNIVAQLKKNNRTEYLQHREIFRPWGSHDTIAEGQRFQVKHVIVLPGHITAKQI
                     HYHRTEHWIVVSGTAKVHLEDKTYLVSENESTYIPIGIPHAIENPGKIPLEIIEVRSG
                     VYLEEDDVIRVSSSGVGY"
     gene            14717..15463
                     /locus_tag="OO135_002121"
     CDS             14717..15463
                     /locus_tag="OO135_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5397961.1"
                     /translation="MRISIITATYNSEKTLLDTLLSLEKQTHPDIEYIVVDGASKDNT
                     IKLIKSNCTRVSKIICEPDNGIYDALNKGIQAASGDVIGFLHSDDLLAYDDVIADIAK
                     AFERSGCDAVYGDLEYVAQNDTTKRIRLWKSGAFSRLKMKLGWMPPHPSFYMKRDCYG
                     QFGCFSLDYRISADYDSLLRYMLKQRISITYLPKVLVKMRVGGISNRSVSSMVNKSME
                     DIRVMKHNGIIWPIALAYKNLSKLPQFIKK"
     gene            15469..16893
                     /locus_tag="OO135_002122"
     CDS             15469..16893
                     /locus_tag="OO135_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCR5397962.1"
                     /translation="MLNAKKIITGSNIAFGTSGARGLVVDFTHDVCAAFTHAFLSVIQ
                     NNFNFRIVALAIDNRPSSYEIAQACAYAIKQHGFEVEYHGVIPTPALAHYSMQKNIPC
                     IMVTGSHIPFDRNGLKFYRPDGEITKEDEFAIVNSEYTFSPVGVLPHLETSSQGADCY
                     LERYVSLFNSDILKGKRIGVYEHSSAGRDLYAPLFNQLGAEVISLGRNDEFVPIDTEA
                     VSDEDRILAREWSKKYNLDAIFSTDGDGDRPLVADENGEWLRGDILGLLTAIELNIKA
                     LAIPVSCNTAIEQSNKFASIQRTKIGSPYVIAAFAALGKQFDSVAGFEANGGFLLASD
                     LQINGKELNSLPTRDAVLPALMLLIASRNSTISQLVNNLPQRFTWSDRVKNFPSESSQ
                     QIIKNALSAPNKFLNRLGYESLSCSAIDETDGARFTLTNGDIIHLRPSGNAPELRCYS
                     EASNENQAKQYVTKVLGNITSLIS"
     gene            17087..18493
                     /gene="gndA"
                     /locus_tag="OO135_002123"
     CDS             17087..18493
                     /gene="gndA"
                     /locus_tag="OO135_002123"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5397963.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLEKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKDAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPKIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            18734..>19194
                     /locus_tag="OO135_002124"
     CDS             18734..>19194
                     /locus_tag="OO135_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5397964.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     VVDKEIQNFLQSDEIRFNATLDKKEAYRNADYVIIATPTDYDPKTNYFNTSSVESVIQ
                     DVIEINPFAVMVIKSTVPVGFTAAMQTKYHTENIIFSPEFLREGKALYDNL"
BASE COUNT         5910 a         3129 c         3975 g         6180 t
ORIGIN      
        1 tgagtaatga taaatgtcac ccttcgatca tggataaatc agggccaaaa tgcaatgtta
       61 cagcaccatt tatccgacat tatcctggag cttatcggca caatatccat catttgttgt
      121 acgtgagaaa attataaatc tggttaacaa atgtcgtgtg gggagatgtt gtattaaaag
      181 tcaccagcct aaagaatgta ctaaaaagcg tcgtgaaata cgcttcatat aattcctgaa
      241 aatcgctaat ggttttacct gataatccat tagttgtaac actgcctccc gcattactgg
      301 agagtcaata ttcaaatacc ttaatcacca tccagcgttt cttgcggaat ggataacggg
      361 atacattctg gcaggataaa acgttgttgt gacgaatatc acaaatctgg ctgctgaccg
      421 ctactccatg ttgaggatga ctggcattcc accatttgca atatcacgcc agatcccagt
      481 aggaaaatgg ggattgataa tacaaaggct tgtatttgca atggtgataa tttcgtggtt
      541 gataatattg atgtgatgaa aaatgtggag atgttaatcg gctacagagc gattaaaagc
      601 aaatacctct ccatactgca aaactttgga gcaggtaata ttcatcttga taatgctaac
      661 cgtgtctggg aattacgcag aatctaaatc ttcgcaggaa atcccccctt tatcgtgctg
      721 acccgcattg aaatgaagtg tgctttgttg gaacaatacg acagattttg acttgttttt
      781 tattaaatat caataatata tttgtattcg tcgctcgtca gtattgacca tgagattagc
      841 cctgcgcgaa gacgtctgtg actggtttat ggcaaagggc gaaacacgct cgttgctgga
      901 aaatgttcat gctgtgaatg aaaagaacaa agctccgtcg atatcgacag aggtaatcac
      961 cgtattatta atgtggaaaa gattaacttt cgacagccag aagaggggag gagatttgcg
     1021 accattcctg gaaaaatgga accatactta aaaatatcgc tactgcaatc ctgaacgaag
     1081 cggcgtagca tcggcagtaa atcagtattc agttcaggat aattgatgct ggcgaaaaaa
     1141 atcgaacgag ctataattca gcaaccattt cacaggtgga tgaaataatg acgaatttaa
     1201 aagcagttat tcctgtagcg ggacttggga tgcatatgtt gcctgccact aaggcgatac
     1261 ccaaagagat gctaccgatc gtcgacaaac ccatgattca gtacattgtt gacgaaattg
     1321 tggctgcagg gattaaagaa atcctcctgg tgactcatgc gtccaagaac gcagtcgaaa
     1381 accacttcga cacttcttat gaactagaat ctctccttga actgcgcgta aaacgccagt
     1441 tgctggctga ggttcagtcc atctgcccac cgggcgttac cattatgaac gtgcgtcagg
     1501 gcgaagcttt aggtttgggc cactccattt tgtgcgccag acctgccatt ggcgacaacc
     1561 catttgtcgt ggtactgcct gatgtcgtta ttgatgacgc cagcgccgat ccgctgcgtt
     1621 acaaccttgc cgccatgatt gcgcgattca acgaaacggg ccgtagccag gtgctggcaa
     1681 aacgtatgcc gggtgacctc tctgaatact ctgtcatcca gactaaagag ccgctggatc
     1741 gtgaaggcaa agttagtcgc attgttgaat ttatcgaaaa acccgatcag ccgcagacgc
     1801 tggactcaga cattatggcg gttggtcgct atgtgctttc tgcggacatt tggcctgaac
     1861 tggagcggac tcaacccgga gcatgggggc gtattcagct aactgacgca attgctgaac
     1921 tggcgaaaaa acagtccgtt gatgcaatgt taatgacggg tgatagctac gattgcggta
     1981 agaaaatggg ctacatgcag gcatttgtga agtacggact acgtaacctg aaagaaggag
     2041 cgaagttccg caaagggatt gagaagctgt taagcgaata aaaatcttac cggatgtaac
     2101 ggtcaataag aaaaaccata acggcagtga agatttgtgg caaaagtaaa ttgcaacaaa
     2161 tgttcctgtc attgtttcgt tataaaacac tataataaca atgagttagc aataagattt
     2221 tagtcaaagt tttcctggat ttttcttgtt tccagagcgg attggtaaga taatcgacgg
     2281 ttcgatttct tgggggttag tgtgtggata acgcacgtta aattgtagac tgcatataca
     2341 gtgctctggt agctgttaag ccaggggcgg tagcgtatat aaattaatta tatggttatt
     2401 aagaatattt tttggcaatt tgcagagaag attttaaggt taatttttgc gatttttatt
     2461 agtgcgttgt tggcaagaca tttaggtgtc agtggctttg gtctgtataa ctatattaat
     2521 gcgattattt ccgttgcatt agttgtaact tcgctcgggt ttaatcgaat tttagttaga
     2581 gaatccactt gttgcaaaga taaaattagt cacaatgagt tgatatcaac atcgttttat
     2641 tcaagactgc ttttttcaat ccttatattc gctctcttat ggggggggta cagctatata
     2701 gataataaaa actcaataat atatttaata tccttccttt cagtaatttt tgtttcattt
     2761 gatgtaattg actatcactt acaaggacag tgtatattta aaacagcaac tctatgtaga
     2821 gttgtgagtt ttttaattgt gtcctttata agaatttttt tagttttcga aaatgcaacg
     2881 ttgatatggt tctgtattac catattattt gaatatgcca tatctgccat tttattatat
     2941 ttgagctcta gatttataca tagaaacatt tttactatta agataaaaca cgtatcacta
     3001 aataaagcaa agtatttgct gaaggaatcg tggtcagaaa ttatagcagg atttggagcc
     3061 attctgttta tgaaaatgga tcaaatcatg ttacaatata tgcttaatgc tgagagtgtt
     3121 ggtgtttatt ctgctgcctt aaggataagt gaatcatggt attttttgcc ggcagcaatt
     3181 gtttcgtcag tatttcctgt aattatgaat aaatataata aggaaaatac aaatgagtcg
     3241 gcgttggttg aattatttgg tcatattatg acccttcttg tctggttatc tatctgtgct
     3301 gcgattacta tttctttgtt tggcccagcg gtaatacatt ggttatttgg tgatgaatat
     3361 attagaagtg ccacaatatt gatatatcat gtatgggcag gagttttttt gagcatgggg
     3421 attctttctg gttcatggtt agtagctaaa aaaaaattaa aattaaattt ataccgaaat
     3481 atcggaggtt tgatagtaaa tttttttcta aatatcatat taataccagt gtatagtggt
     3541 gttggagctg ctattgcaac aatttcagga tttgcattcg ctttcttgtt ttttgatttg
     3601 tttcataaag agttaagggg gatgtttttt ttaaaaataa aatcactttc cccgattaat
     3661 ttaattcaag caatcaggtt tttatctaac ataagattaa aatatctata gtttataatc
     3721 atatgtcatg attggggcat atcacggtgc gccataggca caaaggtcag ggaaagatca
     3781 tcagaggtac tctttttgta ctaaaaaatt tgcatttcat actcactaat tgagatattc
     3841 cttattacct aaactgtttt tcatagctta tatattatca aatactcctt ttatgaataa
     3901 ggagaacaaa atggaactta aaaattgatg gagcatattt ctgctatcct cgattacaga
     3961 caagcttgga aagtggaata taaattgtta gatattctac tgttaactat ttgcaccacc
     4021 atttctggtg cagaaggttg ggaggatata gataatttgg gtgaaacata tctcgatttt
     4081 ttgaagcaat atagttgatg ttgaaaatgg tatttctgtt catgatatca ttgcgagagt
     4141 tatgtcctgt attactcttg ctaaatctta tgaatatttt attaactgaa tattggtata
     4201 tttatatgtt gactgtttaa accaagggca gggtatttaa tatattaaac atatctacgg
     4261 atacaggtta taactattct tgttgttttt tttgaaattg attttctgtt ataactcctg
     4321 attaaagcaa gtgttttgaa atgtgatttt ctagttataa ttcttcgata aaacatagtt
     4381 ctatttccag tatgttatga gtcaaaactc tttaaacaaa gataaataga ttaacttaaa
     4441 ttgcatataa agtgatatta tgaatatgaa aaatataatt gaaagaatta aaccattgtt
     4501 ctttgatggc tccagacact tggtgagacc tgttgtttta cagttcccgg taattgatat
     4561 ttgtaattca aaatgccaaa tgtgtagaat ttgggaaaat aaaaaaagtg tagacatttc
     4621 tgtagagcaa ttaaaaaaag ggatacagaa tgagctattc aaagatgtta tgtctattgg
     4681 gtttaatggt ggagagccca ctcttcgtga ggatttatcg gaggttatag aaacctgcct
     4741 atattctttg ccaaagctaa aacaaatatc attgattacc aacggattta aatatgatca
     4801 agtcattcat cagattgaaa aaatcggtaa gttgattaag tctaaagata tattttttga
     4861 tgtaatggtt tctttggatg gttttggtgt tatccatgat aaagtacgag gaagaactgg
     4921 aagttttaaa catgctcaac atgtattaac ttttctaaaa aacgctgatt atgtaagcaa
     4981 tatacgaatt ggttgtacag taataaaaga aaatgtttat tacttgcatg agttgttgga
     5041 gttttgcata agagaaaagc tgtacgtaaa atatagacaa ggcgtgccac acaaacgatt
     5101 atacacagaa aatttaattg aaccatattc tttaaccttt gaagaaaaat atgagttcgt
     5161 tgagtttttg gagggaataa taaaatatta tgagccaagt ttattacaga aacattttta
     5221 tcgctcatta attaatcaaa tcatatatga gcatccaaga acagcaggat gtgattggag
     5281 atatcgtggt gcgacgataa cagcgaaggg tgaattggca tattgtgcaa tagaaagcaa
     5341 aacgctgatg aaggatatat ctgatggtaa tgcatatcaa atatattttg ataatgaaaa
     5401 tcatttgaat gatattaaag aaaataagtg taaaaactgc catcatgatt atgttggtct
     5461 gcctcctcct gctgaataca gaaaaatatt tctatcaagc ataaatgaaa agtggaaaat
     5521 taaagataaa ataaaaaaaa ttccgggagg gaggggcatt aataagataa taaatgaata
     5581 caagtatagt acatgtataa atcaatatcg aaatattcat tctactcaag ttcaagatgt
     5641 atcattacgt ggaaacacaa gggtgctaat ttgtgggtgg tacggaactg aaactttggg
     5701 agataaagct atcattggag gcattatcaa ttctgtgcgc agtagtttag gttatgaggt
     5761 tgttttttat atcgcatcat tatatccata tatcacagaa atgactcgcc ggcagatgcc
     5821 tgaattgaaa aactgccaaa tagtaaatat acaacaagca atcttgttag caaaagatat
     5881 gacttatgtt gtttttggtg gggggccatt aatggcgata gatgatattg ctccaatgca
     5941 ggttatattt gaaaaagcga aacaagcaaa agtaaaaact ggaataatgg gatgcggaat
     6001 agggccttta ggtgataaga gatataataa tagcattaaa actattcttg agctttctga
     6061 ttttcgtata tatcgagatc aaaaatcaaa aagtgaagca ttaaggttag gagtaaatgc
     6121 tgaaaatgat attgttgccg aggatccagc attttcatgg ataaaatctt attatgaaca
     6181 gtcggtagtg ggtggaagac atcgtaaagc gtatatcaat gatgaaaaag aaatattatt
     6241 attaggttta agagattttc catggagaga gtatgcaaaa catctttcag aaaaagaatg
     6301 cttaagcatt aaagataatt ttgaggaagt attagtatca gctctaaaag aagtagtaaa
     6361 aaagagggat aatttaataa ttagaccatt acctatgtgt actaatcact ttggttcaga
     6421 tgatcgttgg ttttatcgtc gattatttag agattctggt attccatcag aaaatttaga
     6481 tttttcttta tgtggtagag aattagcgcc gactgaatat tgtaaggcat ttttggatgc
     6541 caatgctctt cttgctatgc ggttccattc cttagttttt ggacttggat taggtactcc
     6601 atcgttagct cttgattata cgttgggaaa gggaaaggtc aaatcattgg cggagcgttt
     6661 tggtgctaat gttatatcgt tatctaaaat aaataaagaa gaactactct tagaaataga
     6721 agacttattg gaacataact cacaggaaag taatgtctct attttagaaa gtattaaatt
     6781 tcagcaaagc gtagatacct ttatgaagag ggattaaatt gaaaattcta catatatgct
     6841 acagtgattg tttaggtggg gcagccagag ctgcaaaccg tttacatcag gcacaaagaa
     6901 aactgggaat aaatagcttt atgcttgttg ttgataaata tacagatgat gattatgtct
     6961 tggagcttaa caagctaaca aaatttcgaa taaaaatact taatatgatt tctggctaca
     7021 tattaaggca cctaatacaa aagaatcctg tgaagcactc gttaaatatt ttttcagcag
     7081 gggttgtgaa tgcaataaat aaattaaacc ccgatatagt taatattcat tggattggtg
     7141 ataatatgct ttcaatcaaa gaaatatcaa aaataaaatc gcccataata tggacgatgc
     7201 atgatatgtg ggctttttct ggttgtgaac attatgatag ctttcctgga ataagacgtt
     7261 atgaagatga ctataacaaa aaagatggga tatttgatct aaatagaatc atttatcgat
     7321 ataaaaagaa aaaatggtcc catataaata tgcagtttgt ttctccaagt cggtggttgg
     7381 ccgagtgtgc ctgtagaacg agtctcatag aaggaaaaag agttcatgtt attcagaata
     7441 caatagatca tgttgtattt gataaagtgg ataaagtatg tgcaagaaaa ttgttgggcc
     7501 ttccccaaaa ccgaaaactt atcctatttg gagcgatgtc tggtactgca gatcatcgaa
     7561 aagggtttca tttgctaaat gaagcgttga aatatttatc ggaaaaatat gctactgaat
     7621 atgggcttgt tatttttggt gataaaaaag atggcgatga tatccatgga ttcccaactt
     7681 atagattagg tacattttat gatgagctat ctataaagtt gatatattca gccgcggatg
     7741 tatatgttgc accttcttta caagataatc tacctaatac attagtcgag tcattggctg
     7801 tggggactcc atgtatcgct tttaatattg gaggtatgcc tgatttaatt ataaatagca
     7861 aactaggggg attagtcaca gaggttgata gcagagcatt atctattgct attttggatg
     7921 tactaaatag aaatatagat tctaatataa ttagaaatat ttcgttagca gagagggccg
     7981 aggataatat tgctcataaa tatgaggatg tgtatctcaa tgcataaaaa actatattat
     8041 tactttttaa ttgtttcacc tttgatatat ctaacaggtg gtattgaaca tgttaccccg
     8101 ttatcaatag cggggtattt ggatttaccg tcattgtctc tgattaatta tatattcttt
     8161 ctatggtgtg ttttttttct gcacttttat tcatttggga aaaatatatt atggttgtat
     8221 cttttagata tagtaataat aatgattata tttagagaaa gtagagttga tagcatcttt
     8281 ttttctactc tgtctcaatt tttaattctt tcaccattgt ttcttcttgt cggttgtaaa
     8341 attcatattg gatatgaaag gatcaaaaaa attagctata tatttgtttt ttatttaata
     8401 tgtagtatac ttcttcacta tatatttatc atgtactata aatatatagg tttttcttta
     8461 ccaaatgaga gagcagtagg tatttttaaa aacccaaatc atttagctgc tttttctata
     8521 ctcttactaa tttttctcta taatttagct tttattaaac tagttgacgt aaaaaaaatt
     8581 ttctttgctg agctcattgt tgcagttact gtttatttat ctggtagtag gtcttcgcag
     8641 gtttttttta taatattatt attgatacat tcgttcttgt ataacaaacg tttcttttct
     8701 ttatattgct gtttagccgt tttgtctgtt gcttatgtag gtatgtcaga tatcggagta
     8761 gataagatag aacaattgtt aacaaagcga gagacgaaag atattgctga ggctggtaat
     8821 atgcggatgt cgattctttt tgatatgctt gagaatttta attttcgcga aatattaata
     8881 ggcaagggta gtgggcaagg aactgctgta tatataacta accaaagtaa taataatgag
     8941 aaaattacgt ggttggacag taatgttaat acactaacct atacgtatgg ggttgttttt
     9001 ttaatttttt cattggctgc aatgctgtta aaaatgcttt tcttagctct tatgaaaaga
     9061 acaggtgaat tttatatatt tttcttcgtt ttttatttta tgtggtttat taacattggg
     9121 gaattttttc ctcttatttt tatgttgtta attcaagtaa gtacatctaa tatgggacag
     9181 cagcatgata tcaataatta ctataaatta taataataaa aacgaacttt atcgtacatt
     9241 atcatcaatt gatagtcaga aattaaagag tcatatagag gttattgtta tagatggtgg
     9301 ttctaccgat ggttcgcaag aaatagtatc taactcattt aaacatttgg ttacatattt
     9361 catttcagaa cctgatatgg gtatctatga tgcgatgaat aaaggtatac tattgtctaa
     9421 taaagaatgg ctgtactttt taaatgcggg agatattttt ttctctgatg atgttttaaa
     9481 taaattatat gactttatta taaatagtaa tttgactaat ttgatatatg caccttatat
     9541 tagtgatggg aaaatagata atactcaact tttttctttg tcctatcttt cttcccatat
     9601 gattaatcat caaagtatgc ttctacacaa ggaactgttt caaaaacagt tatatgatat
     9661 taactatagg ttttgtgctg attatgcatt attattaaat ttgtttgata atatcaaacc
     9721 ggtaaagacg gactttatta ttgttgatta tgattcgaaa gggatcagtt cattagatga
     9781 taataaagta tttatatgga aagaaagaat tcttgctgtt ctgaaatcaa acttaagctt
     9841 ttataaaaag ctatttttat tgaaaagggc ttttggggta tacccatatc atttattaaa
     9901 aagtaaatta aaatgcatga agaatataat gacacattgt cgatagtgtt ttgtgcttta
     9961 tatgatgact ggttgaaaaa taaagaactt atacaccgct catttgagca tttcgtaaaa
    10021 atatgggagg agcgatgttt ttttatttta attatacaag atgcgaaaca gcgaaagatt
    10081 gagttggcat ttaaaaacat gcatattttt tatatgcaag aaagaggtat ttcgttagca
    10141 agaaatcgag gtgttgagga agcatgcaca ttaaacagtc gttggattat attccatgat
    10201 gcatctattt gctggactga agaagctgtg ggctttctat ttaaaaatag attctccaaa
    10261 atttcgccta aagttaaact aaaattccaa ttaattgagg aaaagccatc aacagatgtt
    10321 caaggtgaaa taagaaaaat taatccagtt tatgatacat atgttggttc tgttttattt
    10381 gaagttcagt caatcgctaa aataagattt aacatatttc atgggcctgg tcagaatact
    10441 atttataaaa gcggggagga tgttctattc ttttttgatt atttttctat gaagaaaaat
    10501 ttttgtgtgt atgagtctaa aacagcgtat atttatcatc caccacgaga aaaagatttt
    10561 tctaaacata aaatatatgc gaaaggacaa gggcgggtct ttaaaatttt attaaaagaa
    10621 tatttttcaa ttcgtttgct aatggattgc attctttttt ttgggaatgc gcttgtgaga
    10681 tgtatttttt ttaggaaaaa ctctttttat attcttaaag agagatgtgt tggttttttt
    10741 tgtgaggatt aaaatgaaaa aagcattaat tacaggtatt actggtcaag atggttctta
    10801 ccttgctgaa tttcttctcg aaaaaggata tgaggttcat ggtataaaac gtcgagcatc
    10861 ttcttttaac accgatcgcg tagaccatat atatcaagat cgccacaatg atagtccaaa
    10921 ttttctccta cattatggtg atttaacaga ttcatcaaac ttgattcgtc tcattaaaga
    10981 aatccaacca gacgaagttt ataaccttgg cgctcaatct catgtagcgg tttcattcga
    11041 atcacctgaa tatactgctg acgtagatgc gatggggact ttacgtttgc tggaggcgat
    11101 tcgtatttgt gggctagaga aaaaaacacg tttctatcag gcatcaactt ctgaactctt
    11161 cggcttagtt caggaaaccc ctcagcgaga aacaacacca ttctaccctc gttcgcctta
    11221 tgctgttgcg aagatgtacg cttactggat tactgtaaac tatcgtgaat cctatggaat
    11281 gtacgcctgt aacggtattc tcttcaacca tgaatcccca cgtcgtggtg aaacattcgt
    11341 aacacgtaaa atcactcgtg ctattgcaaa tatttcgcag ggaatcgaaa aatgtctcta
    11401 ccttggcaat atggattcac tgcgtgactg gggacatgcg aaagattacg tacgtatgca
    11461 gtggatgatg ttgcagcaag atcaccctga agattttgta attgctaccg gtaaacaaat
    11521 ttctgtccgt gaatttgtcc gaatgtcagc tagagaagtt ggcctagagc ttgagttctc
    11581 tggtgaaggt gttgatgaaa tcgcgaccgt tgtaaataaa acatctgact gcgcgattgg
    11641 cgtaaatatc ggcgatgtta ttgttcgtgt cgatcctcgc tacttccgtc ctgctgaagt
    11701 tgaaactctt cttggtgatc caagtaaagc taaaaaagta ttgggttggg agccagagat
    11761 tacggttgaa gaaatgtgtg cagaaatggt tgctagcgat ttggcgaaag caaaacagca
    11821 tgcactactc aaaagtcatg gttatgatgt tgcggtttct ctggagcgtt gaggtatgac
    11881 aaagaaacgt atctacgttg ctggtcatcg aggtatggtt ggctctgcta tttgccgtca
    11941 attatcactg cgtgatgata tcgaattagt ggtcaaaaca cacaaagaac tcgatctgac
    12001 cgtgcagaag gatgttgagg tattttttga gcaagagaga attgatcaag tttatcttgc
    12061 tgcggcgaaa gtaggaggca tttatgccaa taatacattt ccggcagaat tcatctacca
    12121 gaatctcatg attgagagta atattattca ttcagcccac aaagctggga ttcaaaaatt
    12181 actttttcta ggctccagct gtatttatcc aaaatttgca gagcagccga tgaaagagtc
    12241 tgcgctttta actggaatac ttgaaccaac taatgaacca tacgcaattg ctaaaattgc
    12301 gggcataaaa ttatgtgaat cttataatcg tcaatatgga cgtgattatc gcagtgtaat
    12361 gcctactaat ctttatggta tgaatgataa tttccatcct gaaaattctc atgtcattcc
    12421 ggccctcatg cgtaggtttc atgaggcaaa agagagtggt gcaccagaag tcattgtctg
    12481 gggaacggga acaccgatgc gtgagttttt atatgttgat gatatggctg ccgcgtcggt
    12541 tcatgtaatg gaactagacg acgtaattta tcaacaaaat acacagccaa tgttatcgca
    12601 tattaatgtt ggtactggtg tggattgttc catacgtgaa atggctgaaa caatggcttc
    12661 tgtagtaggt ttccagggaa aaattgtttt tgactcaacg aaaccagatg gtacaccgcg
    12721 taaacttatg gacgttactc gactcaaaaa tttgggttgg caatatcgct ataatttaca
    12781 tgaaggttta tcattaacgt ataaatggtt tcttgcgaat attaattctt ttcggggata
    12841 gctatgaaaa agagattaga atatgagtta tttaaaacaa tagttgagca tactcctcta
    12901 atctcgattg atctcataat tagaaacgat aaaggagagg cgctgcttgg gcagcgcctg
    12961 aatcgcccgg cacaaaatta ttggtttgtg ccgggggggc gaatttttaa agatgagtca
    13021 ttcgaggcgg cttttaaacg gataacattt gaagagttgg gcgttcaaac cagtcttaat
    13081 gaagccttat ttcttggtgt gtatgaacat ttttacaatg ataacttttc tgaaacagaa
    13141 ttttctacac actacgtagt acatgggtat gaaatccaac taaatccaca gcaacttcac
    13201 ctaccaacga tccagcataa ctcctacaag tggtttgata tagaaacgtt gcttaatagc
    13261 actgatgttc atcaatacac caaaaattat tttatttcaa ggtaatagat atgcttcttc
    13321 ccgttgttat ggccggtggt tctgggacca gattatggcc tctttcacgt acgctttatc
    13381 ccaaacagtt tctgtcttta aatagccgtt tgaccatgtt gcaagagaca ttgcgccgac
    13441 ttgacaaggt cgaacataaa cctactttgg tcatttgtaa cgaatcacat cgctttatcg
    13501 ttgctgaaca attgcgtaaa gagggtttaa agcatagcgg tattttgctt gagcctgttg
    13561 gtcgtaatac tgcgccagct gttgcgttgg cagcactcca ggcaaaagca tctggcgaag
    13621 atcctatact ccttgtactt gctgctgatc atgaaattca ggatgaaggt cgctttattg
    13681 ctgctgttct tgcggctaag aattttgctg aacagggtaa gcttgttaca tttggtattg
    13741 ttccagctgc tcctgagacg ggttatggtt acatcaaggt tggtgaggag ctagatgggc
    13801 agggttacaa agtcgcggcc tttgttgaaa aaccagagtt ggatttagct aaacagtatc
    13861 tggccgatgg tggctatctt tggaatagcg ggatgttcat gttcagggca tctatatttc
    13921 ttgatgaact gaaaaagttt cgtccagata ttctggccag ttgccaacaa tccctgtcct
    13981 cttcgacaca agatttagat tttattcgcc tggataactc ttcattttct tgctgtcctg
    14041 aagagtctat tgactatgct gttatggaaa aaacagccga agctgtcgtc gttccattaa
    14101 atgcgcaatg gagtgatgtt gggtcatggt ctgctctgtg ggaaatcagt tcaaaagacc
    14161 aaagcggcaa cgctattcgt ggtgacgttt tgattgaaga tgctacagat agctaccttt
    14221 attcgcagca taggcttatt ggtgctgttg gtgtaaaaga tttggttgtt gttgaaacga
    14281 aagatgcagt attagttgct cataaagaca aagttcagca agttaaaaat attgtcgctc
    14341 aacttaaaaa gaataatcga acagagtatt tacagcatcg ggagattttt cgtccttggg
    14401 gcagccatga cactatagct gaaggacaac gctttcaggt aaaacatgtg attgtattac
    14461 ctggtcacat cactgctaag caaatccatt atcatcgtac tgagcattgg attgttgtct
    14521 cggggacagc taaagttcat cttgaagata agacttatct tgtttcagaa aatgaatcaa
    14581 catatatacc tattggtatt ccgcatgcca ttgaaaaccc tggcaagatc ccacttgaaa
    14641 taattgaagt tagatcagga gtctacttgg aagaggatga tgtcataaga gtttcttctt
    14701 ctggagtcgg atactaatgc gaatttctat tatcacggca acctataata gtgaaaaaac
    14761 tctccttgat acattactct ctctggaaaa acaaacacat ccagatatcg aatatatagt
    14821 tgtagatggg gcatcgaaag acaatacaat caagctaatc aagagtaatt gtacaagagt
    14881 ttcaaaaatc atttgtgaac ctgacaatgg catttatgat gcgctgaata aaggtattca
    14941 agctgcttct ggtgatgtta ttggtttttt gcattctgat gacttacttg cttatgatga
    15001 tgttattgca gatatagcaa aagcatttga acgttcaggg tgtgatgctg tttatggtga
    15061 tttggagtat gtcgcccaaa atgatacgac taaacgtatt agattatgga aaagtggagc
    15121 atttagccgt ttgaagatga aactgggttg gatgccacca cacccatcat tttatatgaa
    15181 acgtgattgt tatggtcagt ttggttgttt ttcattagat tatcgaatat ctgctgatta
    15241 tgactcattg ttacgttata tgttaaaaca gcgtatttca ataacgtatt taccaaaagt
    15301 attagtgaag atgcgtgttg gcggaattag caatcgctca gtatcttcca tggtcaacaa
    15361 gtcaatggaa gatattcgtg ttatgaaaca caatggtatt atttggccaa tagctttagc
    15421 gtataaaaat ctatccaaac ttccacaatt cattaaaaag taattctcat gttaaatgct
    15481 aaaaaaatca ttaccggtag caatattgct ttcggaacta gtggcgcgcg tggtctcgtt
    15541 gtcgatttta ctcatgatgt ttgtgcggcg ttcacccacg ctttcctttc cgttatacag
    15601 aataacttta acttccgtat tgttgcctta gccattgata accgcccaag tagttacgaa
    15661 atagctcagg catgtgcata tgctataaaa caacatggat ttgaggttga atatcatggt
    15721 gtcattccga ctcctgcatt agctcattat tcgatgcaga aaaatattcc ctgcataatg
    15781 gtcactggaa gtcatatacc ttttgatcgt aatggtttga aattctaccg accagatggt
    15841 gaaatcacaa aagaggatga gttcgcaatt gtaaatagtg aatatacatt ttctcctgta
    15901 ggtgtattac ctcatcttga aacaagctct caaggtgcgg actgctacct ggaacgttat
    15961 gtttctcttt ttaattctga tattttaaag gggaaaagaa taggggtata tgagcattct
    16021 agtgcaggac gagatctata tgcacctctt tttaatcaat tgggggctga ggtcatatcc
    16081 cttggtagaa atgatgagtt cgttcctatt gatacggaag ccgtaagcga tgaagatcgt
    16141 atacttgcgc gagagtggtc taaaaaatat aatcttgatg ctattttttc aacagatggt
    16201 gatggtgatc gtcctttagt tgccgatgaa aatggtgaat ggctaagagg cgatattctg
    16261 ggattactta ctgctattga gcttaatatc aaggcgttgg ctattccagt gagttgtaat
    16321 acagcaattg aacagtctaa taaatttgca agtatacaac gaacgaaaat aggttctcct
    16381 tatgtaattg cagcgtttgc agctcttggt aaacaatttg attcagttgc tggttttgaa
    16441 gctaatggtg gttttctgct cgcctctgat ttacaaatta atggtaaaga attaaattca
    16501 ttgcctacgc gagatgctgt attaccggca ttaatgcttc taatagcttc tcgtaatagt
    16561 actatctctc aactggttaa taatcttcca cagcgattca cttggtcaga tagagttaaa
    16621 aacttccctt cagagtcaag tcagcaaatt ataaaaaatg ccttatcggc acctaataaa
    16681 ttcttaaata gattagggta tgaatcatta tcctgttcag ctattgatga aacggatggt
    16741 gcaagattta ctttaactaa tggtgatatt atacatctcc gtccttctgg gaatgcccct
    16801 gaactccgtt gttattctga agccagtaat gaaaatcagg ccaagcaata tgttacgaaa
    16861 gtgcttggaa acattacctc tttaatttct tgatgttata ggtttatcta cgcttatatg
    16921 tgtgcgtagg tttgattaca agtggatgcc ggtatacagg atgaagaacg gtattcgttg
    16981 cattaatgaa attcagcact acacacattc gtgcaacttg agataacatc tcaatcacat
    17041 tcaagccgcg caaccccgcg gtgaccaccc ctgacaggag taaacaatgt caaagcaaca
    17101 gatcggcgtc gtcggtatgg cagtgatggg gcgcaacctt gcgctcaaca tcgaaagccg
    17161 tggttatacc gtctctattt tcaaccgttc ccgtgaaaag acggaagaag tgattgccga
    17221 aaatccaggc aagaaactgg ttccttacta tacggtgaaa gagtttgttg aatctcttga
    17281 aacgcctcgt cgtatcttgt taatggtgaa agcaggtgca ggcacggatg ctgctattga
    17341 ttcccttaag ccataccttg aaaaaggcga catcatcatt gatggcggta acaccttctt
    17401 ccaggacacc attcgtcgta accgtgagct ttctgctgaa ggttttaact tcattggtac
    17461 gggggtttcc ggtggtgaag agggcgcgct gaaaggtcct tctattatgc ctggcggtca
    17521 gaaagatgcc tatgaactgg ttgcgccgat cctgaccaaa attgctgccg tagctgaaga
    17581 tggcgaaccg tgcgttacct atattggtgc agacggtgct ggtcactatg tgaagatggt
    17641 tcacaacggt attgaatacg gtgatatgca gttgattgct gaagcctatt cactgctgaa
    17701 aggtggtctg aatctctcca acgaagagct ggcgcagacc tttaccgagt ggaataacgg
    17761 tgaactgagt agctatctga tcgacatcac caaagatatc ttcactaaaa aagatgaaga
    17821 tggtaactat ctggttgatg tgattctgga tgaagcagct aacaaaggca ccggtaaatg
    17881 gaccagccag agtgcgctgg atctcggtga gccgctgtcg ttgattaccg agtctgtgtt
    17941 tgctcgttat atctcttctc tgaaagagca gcgtgttgcc gcgtctaaag ttctgtccgg
    18001 tcctcaggca cagccagcag gcgacaaagc ggagttcatc gagaaagttc gccgtgcgct
    18061 gtatcttggc aaaatcgttt cttatgctca gggcttctct caactgcgtg ccgcgtctga
    18121 agagtacaac tgggatctga actacggtga aatcgcgaag attttccgtg ctggctgcat
    18181 catccgtgcg cagttcctgc agaaaatcac cgatgcctat gcagaaaatc caaaaatcgc
    18241 taacctgctg ttggctccgt acttcaagca aatcgccgac gactaccagc aggcactgcg
    18301 tgatgtcgtc gcttatgcag tgcaaaacgg tattccggtt ccaaccttct ctgcggcggt
    18361 tgcttattac gacagctatc gtgccgctgt tctgccagcg aacctgatcc aggcacaacg
    18421 tgactatttc ggtgcgcata cttataagcg tattgataaa gaaggtgtgt tccataccga
    18481 atggctggat taatctgatt taatcagcaa taaattgagg cccggcatat attgccgggt
    18541 ttttttgcta ataatcctat agattaaatt tcattgctta atattaactt aataaatatc
    18601 agctactctt ataagtaaag tctgattttg ttgttacgct gatgctggga tcagcgtagc
    18661 gctaaaaatt ttaacttcta tcaggatgct aaaacatcat gattcacagt taagttaatt
    18721 ctgagagcat gaaatgaaaa ttaccatttc cggtaccggc tatgtgggcc tgtcgaatgg
    18781 gcttctaatc gcacaaaacc atgaggttgt tgcattagat attttgcctt cacgtgttgc
    18841 aatgctgaat gatcggatat ctcctgtcgt tgacaaagag attcaaaatt ttttgcagtc
    18901 agatgaaatt cgatttaacg ccacgttaga taaaaaagaa gcctatcgga atgcagatta
    18961 cgtaatcatc gcaacgccta cagactacga tccaaaaaca aattatttca atacgtctag
    19021 tgtggaatct gtcattcagg atgttattga aataaaccca tttgcggtta tggttatcaa
    19081 gtctactgtc ccggttggtt ttaccgcggc aatgcagacg aaatatcata ctgaaaacat
    19141 tattttttcc ccagaattcc tccgcgaagg caaagccctt tacgataacc ttca
//
LOCUS       DAJTMK010000071        19116 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTMK010000071 DAJTMK010000000
VERSION     DAJTMK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19116
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.072"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..191)
                     /locus_tag="OO135_002125"
     CDS             complement(<1..191)
                     /locus_tag="OO135_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5397965.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTV"
     gene            292..891
                     /locus_tag="OO135_002126"
     CDS             292..891
                     /locus_tag="OO135_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5397966.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            999..1766
                     /gene="tpiA"
                     /locus_tag="OO135_002127"
     CDS             999..1766
                     /gene="tpiA"
                     /locus_tag="OO135_002127"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5397967.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(1821..2576)
                     /gene="cdh"
                     /locus_tag="OO135_002128"
     CDS             complement(1821..2576)
                     /gene="cdh"
                     /locus_tag="OO135_002128"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5397968.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(2683..3672)
                     /gene="sbp"
                     /locus_tag="OO135_002129"
     CDS             complement(2683..3672)
                     /gene="sbp"
                     /locus_tag="OO135_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5397969.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(3992..4954)
                     /gene="pfkA"
                     /locus_tag="OO135_002130"
     CDS             complement(3992..4954)
                     /gene="pfkA"
                     /locus_tag="OO135_002130"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5397970.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(5135..6037)
                     /gene="fieF"
                     /locus_tag="OO135_002131"
     CDS             complement(5135..6037)
                     /gene="fieF"
                     /locus_tag="OO135_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5397971.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHMISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(6185..6685)
                     /gene="cpxP"
                     /locus_tag="OO135_002132"
     CDS             complement(6185..6685)
                     /gene="cpxP"
                     /locus_tag="OO135_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5397972.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSDDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            6835..7533
                     /gene="cpxR"
                     /locus_tag="OO135_002133"
     CDS             6835..7533
                     /gene="cpxR"
                     /locus_tag="OO135_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5397973.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7530..8903
                     /gene="cpxA"
                     /locus_tag="OO135_002134"
     CDS             7530..8903
                     /gene="cpxA"
                     /locus_tag="OO135_002134"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5397974.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9009..9683)
                     /gene="yiiM"
                     /locus_tag="OO135_002135"
     CDS             complement(9009..9683)
                     /gene="yiiM"
                     /locus_tag="OO135_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5397975.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(9832..10814)
                     /gene="kdgT"
                     /locus_tag="OO135_002136"
                     /pseudo
     CDS             complement(9832..10814)
                     /gene="kdgT"
                     /locus_tag="OO135_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(11074..11694)
                     /gene="sodA"
                     /locus_tag="OO135_002137"
     CDS             complement(11074..11694)
                     /gene="sodA"
                     /locus_tag="OO135_002137"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5397976.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            11978..13012
                     /gene="rhaT"
                     /locus_tag="OO135_002138"
     CDS             11978..13012
                     /gene="rhaT"
                     /locus_tag="OO135_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR5397977.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13009..13875)
                     /gene="rhaR"
                     /locus_tag="OO135_002139"
     CDS             complement(13009..13875)
                     /gene="rhaR"
                     /locus_tag="OO135_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5397978.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQAWQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(13931..14767)
                     /gene="rhaS"
                     /locus_tag="OO135_002140"
     CDS             complement(13931..14767)
                     /gene="rhaS"
                     /locus_tag="OO135_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5397979.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15055..16467
                     /gene="rhaB"
                     /locus_tag="OO135_002141"
     CDS             15055..16467
                     /gene="rhaB"
                     /locus_tag="OO135_002141"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5397980.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSMTLREIHRFNNDLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            16464..17723
                     /gene="rhaA"
                     /locus_tag="OO135_002142"
     CDS             16464..17723
                     /gene="rhaA"
                     /locus_tag="OO135_002142"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5397981.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            17990..18814
                     /gene="rhaD"
                     /locus_tag="OO135_002143"
     CDS             17990..18814
                     /gene="rhaD"
                     /locus_tag="OO135_002143"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR5397982.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAIPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            18824..>19116
                     /gene="rhaM"
                     /locus_tag="OO135_002144"
     CDS             18824..>19116
                     /gene="rhaM"
                     /locus_tag="OO135_002144"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5397983.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         4722 a         5032 c         4855 g         4507 t
ORIGIN      
        1 ttaacagtta ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc cgcggtctga
       61 atatcgagta aatttttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg
      121 cctatccaaa tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc
      181 tgtatggtca tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag
      241 ggcgacagat atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca
      301 gggtgtacgc tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca
      361 caaacaccag atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat
      421 ctctccatca gccagtttcg agaagacttt aacagccaga atcctagcct gccactgaac
      481 gaatttcgtg ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa
      541 attaacgaga acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc
      601 attcaaatga cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct
      661 caggaataca tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc
      721 cagaaaaaac tgcagtcgct actaacggcg gggaaaaaca aacgttatta caccgagaca
      781 gaaggtgcac tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt
      841 gaaccgatta agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca
      901 aagcctttgt gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc
      961 catcttcctt tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg
     1021 gtaactggaa actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta
     1081 aagagctggc aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg
     1141 atatggcgaa gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc
     1201 tgaacctgtc cggcgcattc accggtgaaa cctctgctgc tatgctgaaa gacatcggcg
     1261 cacagtacat catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac
     1321 tgatcgcgaa aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg
     1381 gtgaaaccga agctgaaaac gaagcgggca aaactgaaga agtttgcgca cgtcagatcg
     1441 acgcggtact gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac
     1501 ctgtatgggc aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca
     1561 aattcatccg tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc
     1621 agtacggcgg ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg
     1681 acggcgcgct ggttggcggt gcgtctctga aagctgacgc tttcgcagta atcgttaaag
     1741 ctgcagaagc ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt
     1801 actttcctta actcttcgcc ttaacgcaaa atctcacact gatgatcctg aatttcctcg
     1861 gctgaagcac ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac aaatgagtta
     1921 tcactctgcc gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggtact
     1981 tcttctgcca gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc
     2041 gccaggtatt catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc
     2101 gccagattgt tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga
     2161 aaatggtttt gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga
     2221 accggctggc cgtacttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag
     2281 ttcggcgttg aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc
     2341 atcaacagat attgcagtgg gccattaaga tcttttaaaa ccacgtatcc ggcattgggt
     2401 ttgacttccg cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca
     2461 aggacaatct tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg
     2521 gcagccacaa cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt
     2581 ttttccctgt acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc
     2641 ggtcgcgaga aaccaccggg tttgataatt atcctgggga aatcagcgtt tgctgatctg
     2701 atcgaacgta ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc
     2761 ttcatcaatg gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc
     2821 agcgtcgcgc ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag
     2881 atatttcagg taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac
     2941 caccgacacg gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc
     3001 tttccccagt tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc
     3061 aatcccgcgc tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac
     3121 gtttttatac agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg
     3181 cagcgcgtag ccccaggcag ccaggtagtt ccagcgtgcg ccaccagagc ttttcggatt
     3241 aggcgtgatc accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg
     3301 attgccctta cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag
     3361 acgtttgatc cactctttat caatccgccc gcgttccgca attgcgtcca cgtcataggc
     3421 cagagccagc gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc
     3481 tgaaccaccg tgtgactgac gcatcaccac gttatcacca gtttgctgtt tccagtgggc
     3541 gctgaatgcc ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag
     3601 aagctgaata tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc
     3661 ccacttgttc atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag
     3721 gttaaccaaa cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg
     3781 gattaaaaag gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc
     3841 ggatgcggcg cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga
     3901 attcatgtag gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca
     3961 ggctaaagga atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc
     4021 agtcaccttt gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca
     4081 gctgttcgtt ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag
     4141 cgtaagcgcc catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct
     4201 ggatgtggcc cagcacagtt gcgcgggttt cacgaccggt ttctttctcg atgaaatgcg
     4261 ccagttcgtc aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt
     4321 tcgcgatacc cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa
     4381 ccacaacgaa ttcacagcca ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac
     4441 ggcccatcac ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt
     4501 cgatcgcttc tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt
     4561 tgatgtcgtt gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac
     4621 gcattgcacc catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt
     4681 ttttcaggtt ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg
     4741 cagaaccgag gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct
     4801 gtaccatacg gtcttcatac agacccagat agccgtcata aatacccatt acttccagac
     4861 cttctgtcag cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac
     4921 cgccgcttgt caacacaccg attttcttaa tcatgactac ctctgaactt tggaatgcaa
     4981 aatgaaatct gttgccggaa gtcttcttgc acatcgcagt gatccaacga atgtgcaaat
     5041 agtataacaa tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata
     5101 cacaaaatgc gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct
     5161 ccctgggtac gacggaacag gggtcctgat ggataattac atccgatccc ggaaaacgcc
     5221 gtaaaatagc ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt
     5281 cttccatttc caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat
     5341 cgtgagcgcc gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttcct
     5401 catcaggcaa tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc
     5461 tatataaaat atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt
     5521 accaggacaa ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat
     5581 gtagcatatc agcccgcacc gcctggcttt gcgtccggcg caccacccaa cgctgaaacg
     5641 agacaaggat aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat
     5701 ctatcatcgg tgttggagat atcatatgtt gaatacccgt caaaaacagg aatagtgccg
     5761 aaccggagat aaacatactt tgcgccagcg ccgcgaggga ctctgcttta ccgtgaccaa
     5821 acgagtgatt atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg
     5881 acgcgccgat atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat
     5941 accaccatgc aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg
     6001 ccgcccgact gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac
     6061 gctagtataa cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa
     6121 aaaaaacccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg
     6181 tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc
     6241 attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt
     6301 gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac
     6361 gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat
     6421 tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt
     6481 cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta
     6541 tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc ggatgccagt
     6601 tatcgtctga accgacttca gcagcgtggc ttaatgaact gactgccagc gttgaggcca
     6661 tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc gcgattcaac
     6721 gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa caacgtaaag
     6781 tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta aacaatgaat
     6841 aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga gctgctcgag
     6901 atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga tcttctggac
     6961 gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg tatcgacaca
     7021 ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc gcgcggcagt
     7081 gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc gaaaccgttt
     7141 aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca ctggagcgag
     7201 caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt agtgctgaat
     7261 ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg tactgagttt
     7321 accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga acatttaagc
     7381 caggaagtgt tgggcaaacg cctgacgcct ttcgaccgcg ctattgatat gcacatttcc
     7441 aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa aaccttgcgt
     7501 ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg cgcatcttcg
     7561 ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta cccaagctcg
     7621 attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg atgattgagc
     7681 agcatgtcga agcggagctg gcgaacgatc cgcccaacga tttaatgtgg tggcggcgtc
     7741 tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg gtgaccaccg
     7801 aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac tttattggtc
     7861 aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa ctggtcggtc
     7921 cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt ccggccagca
     7981 gttctcaatc cgatttcatt aacttactgt ttgaccgccc gctattactg ctgattgtca
     8041 ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca aaaccggcgc
     8101 gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag cacccggaac
     8161 tggaagcggg gccacaggaa ttccttgccg caggtgccag ttttaaccag atggtcaccg
     8221 cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct cacgagctgc
     8281 gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt agcggtgaaa
     8341 gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg atcaacgatc
     8401 tgttggtgat gtcacgtaat cagcaaaaaa acgcgctggt tagcgaaacc atcaaagcca
     8461 accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcaa atgggcaagt
     8521 cgttgacagt taacttcccg cctgggccgt ggccgctgta cggcaatccg aacgccctgg
     8581 aaagtgcgct ggaaaacatt gttcgtaatg ctctgcgtta ttcccatacg aagattgaag
     8641 tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat ggtcctggcg
     8701 ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat gaagcacgcg
     8761 atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc attcagcagc
     8821 atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg gtgatttggt
     8881 tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc gtttacgccg
     8941 catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc aggcctacac
     9001 tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc gctcagtcgg
     9061 cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag taaacggtga
     9121 tactgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg cacggtcaca
     9181 tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc tggcgctatc
     9241 acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat atcgctgata
     9301 tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg aattaatgcc
     9361 tctccccagc ggaaaatatc gcccatataa acattacttt ccgtcaggcc gtcggttgag
     9421 aggttttcac caaacgcagg cgcaacaaac aactccgcct gttccggaaa ttcccgcgcc
     9481 cagtagagat aatgctcacg aggataatga cacagcgctc tgtctggccc accgtgaacc
     9541 tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa ctcaccatca
     9601 acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat cttgcctgtg
     9661 tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc cattagacca
     9721 tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg aactcaatag
     9781 caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg tttatttcac
     9841 cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc agatagaggt
     9901 aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg aagttgctgc
     9961 cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg tcgctaccgc
    10021 ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc caatcaattt
    10081 atcggcgata atcaacggaa taccggtcac gataattact gccacaccca acaggatccc
    10141 cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg cgaaggcaaa
    10201 gaatggaatc agcgtttgca ccgctttgct gaaaaattct cgtaattcag ggtcaaggtt
    10261 ccccagggca aagcccacga ggaacggtaa tactgcgccg acgaaaacat gcggttcaaa
    10321 cgaggcaatc cctgcagtgc ccagaataat catcgtcatg agcgccccgg actccaacga
    10381 catcaacaca aatgccccag cttcttcttt tgtgccgtac tgctgcatga tggaagcgta
    10441 aagtccgcca ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa gtccggcaaa
    10501 gaatccaact tcaacgccat gttccggaat aatgcgcgag gcaattgccg caaccaccca
    10561 cgcgacggca atttttgtta ccaccagcgt accggattta cgcaataccg ttcccgtcgc
    10621 gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg acaccgtacc
    10681 ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg agaaggtgtg
    10741 gcacagtgcg ccaaggaata gcgggacgag catcatcccc ccgggatttt ctcaatcgag
    10801 cgttttatct gcatttccat cacctgtcgg catattttta agggctgatg cgtaattcct
    10861 caatggagca tcagtgttga tggaaacgaa gatagacatt catttaatca ataaaagtga
    10921 tcaaaaccac attaattgaa acgctgtttt atttttatcg gatcattgtt tttattttga
    10981 aattgaacac aaaaaaacca ccgccgttgg cgatggttca ttttttaagc tgatatgcgg
    11041 cgtaaacgcc tcattgcagc aggcggcaaa tgattatttt ttcgccgcga aacgtgccgc
    11101 tgcttcatcc cagttcacca cgttccagaa ctctttgatg tagtccggac ggcggttctg
    11161 gaatttcagg tagtaagcat gttcccacac atccaggccc agaatcggga agccggaagc
    11221 gccagaaata gcttcaccca tcagcgggga atcctggtta gcagtagaaa ccaccgccag
    11281 tttatcgcct ttcagcacca gccatgccca gccggaacca aagcgggaag ctgccgcttt
    11341 ctcaaattct gctttgaagt tatcaacgga gccgaagtca cgttcgatag ccgctttcag
    11401 gtcaccctgt aaggtggtgc cttttttcag acctttccag aacaggctgt ggttagcgtg
    11461 accgccagcg ttgttgcgca gtacggtttt cttgtctgct ggcagctggt ccagtttggt
    11521 gatcagctct tcaaccggca ggttggcaaa ttctggcagg ctttccagcg ccgcgttggc
    11581 gttgttgacg taggtctgat ggtgtttggt gtggtggatt tccatggtct gcttatcgaa
    11641 gtgcggttcc agggcatcgt aagcatacgg cagggatggc agggtatagc tcatattcat
    11701 ctccagtatt gtcgggcggc cgattgttaa tgcgcgtaag cagttggttc attatagtta
    11761 attaaatgat attgaaaatg attatcaatg ctgtactttt cgtaagggta tggttttgca
    11821 ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg ccgtgattgc cagtaaatcg
    11881 acaacggcgg caacaggcga aaggttaatc gacagcacga tttttacact catctcgtcg
    11941 gagatgtgac gcgacgaaaa atgatgagga taagaagatg agtaacgcga ttacgatggg
    12001 gatattttgg catttgatcg gcgcggccag tgcagcctgt ttttacgctc cgttcaaaaa
    12061 agtaaaaaaa tggtcatggg aaaccatgtg gtcagttggt gggattgttt cgtggattat
    12121 tctgccgtgg gccatcagcg ccctgttact gccgaatttc tgggcgtatt acagctcgtt
    12181 tagtctctct acgctgctgc ctgtttttct gttcggcgct atgtggggga tcggtaatat
    12241 caactacggc ctgaccatgc gttatctcgg catgtcgatg ggaattggca tcgccattgg
    12301 cattacgttg attgtcggta cgctgatgac gccaattatc aacggcaatt tcgatgttct
    12361 gcttaatacc gaaggcggac gcatgacgtt gctcggcgtt ctggtggcgc tgattggtgt
    12421 agggattgtg actcgcgccg ggcagttgaa agagcgcaaa atgggcatta aagccgaaga
    12481 gttcaacctg aaaaaagggc tggtgctggc ggtgatgtgc ggcattttct ctgccggtat
    12541 gtcttttgcg atgaacgccg caaaaccgat gcatgaagcc gctgccgcac tgggcgtcga
    12601 tccactgtat gtcgctctgc caagctatgt catcatcatg ggcggcggcg cgatcatcaa
    12661 cctcggtttc tgcttcattc gtctggcaaa agtgaaggat ttgtcgctaa aagccgactt
    12721 ctcgctggca aaaccgctga tcattcacaa tgtgttactc tcagcactgg gcgggttgat
    12781 gtggtatctg caattctttt tctatgcctg gggccacgcc cgcattccgg cgcagtatga
    12841 ctacatcagt tggatgctgc atatgagttt ctatgtattg tgcggcggta tcgtcgggct
    12901 ggtgctgaaa gagtggaaca atgcaggtcg ccgtccggta acggtgttga gcctcggttg
    12961 tgtggtgatt attgtcgccg ccaacatcgt cggcatcggc atggcgaatt aatctttctg
    13021 cgaattgaga tgacgccact ggctgggcgt catcccggtt tcccgggtaa acaccaccga
    13081 aaaatagtta ctatcttcaa agccacattc ggtcgaaata tcactgatta acaggcggct
    13141 atgctggaga agatattgcg catgacacac tctgacctgc cgcagatatt gattgatggt
    13201 cattccagtc tgctggcgaa attgctgacg caaaacgcgc tcactgcacg atgcctcatc
    13261 acaaaattta tccagcgcaa agggactttt caggctagcc gccagccggg taatcagctt
    13321 atccagcaac gtttcgctgg atgttggcgg caacgaatca ctggtgtaac gatggcgatt
    13381 cagcaacatc accaactgcc cgaacagcaa ctcagccatt tcgttagcaa acgacacatg
    13441 ctgactactt tcatgctcaa gctgaccgat aacctgccac gcctgcgcca tccccacgct
    13501 acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat cccggaatcg ctccctgcca
    13561 gtcaagattc agcttcagcc gctccgggca ataaataata ttctgcaaaa ccagatcgtt
    13621 aacggaagcg taggagtgtt tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat
    13681 gcgataaggg cgatcgttga gtacatgcag accattaccg cgccagacaa tcaccagctc
    13741 acaaaaatca tgtgtatgtt cggcaaaaac atcttgcgga taacggtcag ccacagcgac
    13801 tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt tttaactgat gcgccaccgt
    13861 ggctacctcg gccagagaac gaagttgatt attcgcaata tggcgtacaa atacgttgag
    13921 aagattcgcg ttattgcaga aagccatccc gtccctggcg aatatcacgc ggtgaccagt
    13981 taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatccg cagcgatagg
    14041 cgatgtcagt aacgctggcc tcgctgtgac gtagcagatg tcgggccttc atcagtcgca
    14101 ggcggttcag gtaacgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg
    14161 tacgcagtga aagagaaaat tgatccgcca ctgcatccca attcacctca tcggcaaaat
    14221 ggtcctccag ccaggccaga agcaagttga gacgtgatgc gctgttttcc aggttctcct
    14281 gcaaactgct tttacgcagc aagagcagta attgcataaa caagatctcg cgactggcgg
    14341 tcgagggtaa atcattttcc ccttcctgct gttccatctg tgcaaccagc tgtcgcacct
    14401 gctgcaatac gctgtggtta acgcgccagt gagacggata ctgcccatcc agttcttgtg
    14461 gcagcaactg attcagcccg gcgagaaact gaaatcgatc cggcgagcga tacagcacat
    14521 tggtcagaca cagattatcg gtatgttcat acagatgccg atcatgatcg cgtacgaaac
    14581 agaccgtgcc accggtgatg gtatagggct gcccattaaa cacatgaata cccgtgccat
    14641 gttcgacaat cacaatttca tgaaaatcat gatgatgttc aggaaaatcc gcctgcggga
    14701 gccggggttc tatcgccacg gacgcgttac cagacggaaa aaaatccaca ctatgtaata
    14761 cggtcatact ggcctcctga tgtcgtcaac acggcgaaat agtaatcacg aggtcaggtt
    14821 cttaccttaa attttcgacg gaaaaccacg taaaaaacgt ctatttttca agatacagcg
    14881 tgaattttca ggaaatgcgg tgagcatcac atcaccacaa tgaagcaaat tgtgaacatc
    14941 atcacgttca tctttccctg gttgccaatg gcccattttc ctgtcagtaa cgagaaggtc
    15001 acgaattcag gcgcttttta gactggtcgt aatgaaattc agcaggatca cattatgacc
    15061 tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg ggcgcgtgat gctggcgcgt
    15121 tacgagcgtg aatgccgcag catgacgctg cgcgaaatcc atcgttttaa caatgacctg
    15181 catagccaga acggttatgt cacctggaat gtggatagcc tggaaagtgc cattcgcctt
    15241 ggattaaaca aggtgtgcga ggaagggatt cgtatcgata gcattgggat tgatacctgg
    15301 ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg tgggcctgcc cgttgcttat
    15361 cgcgatagcc gctccaatgg cctaatggcg caggcacagc aacaactcgg caaacgcgat
    15421 atttatcaac gtagcggcat ccagtttctg cccttcaata cgctttatca actgcgtgcg
    15481 ctgacggagc aacaacctga acttattcca cacattgctc acgctctgct gatgccggat
    15541 tacttcagct atcgcctgac cggcaagatg aactgggaat ataccaatgc caccaccaca
    15601 caactggtca atatcaatag cgacgactgg gacgagtcgc tactggcgtg gagcggggcc
    15661 aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg tcattgggca ctggatttgc
    15721 ccgcagggta atgagattcc ggtggtcgcc gttgccagcc atgataccgc cagcgcggtt
    15781 atcgcctcgc cgttaaacgg ttcacgcgct gcttatctct cttctggcac ctggtcattg
    15841 atgggcttcg aaagccagac gccatttacc aatgacacgg cgctggcagc caacataacc
    15901 aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa atattatggg cttatggctg
    15961 cttcagcgag tgcttcagga gcggcaaatc aacgatctcc cggcgcttat cgccgcgaca
    16021 caggcacttc cggcctgccg cttcatcatc aatcccaatg acgatcgctt tattaatcct
    16081 gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa cggcgcaacc gatcccggaa
    16141 agtgatgctg aactggcgca gctacgcggt gaagatttct cgcaactgca tattgtcggc
    16201 ggcggctgcc agaacacgct gctcaaccag ctatgtgccg atgcctgcgg tattcgggtg
    16261 atcgccgggc ctgttgaagc ctcgacgctc ggcaatatcg gcatccagtt aatgacgctg
    16321 gatgaactca acaatgtgga tgatttccgt caggtcgtca gcaccaccgc gaatctgacc
    16381 acctttaccc ctaatcctga cagtgaaatt gcccactatg tggcgcagct tcactctaca
    16441 cgacagacaa aggagctttg cgcatgacca ctcaactgga acaggcctgg gagctggcga
    16501 aacagcgttt cgcggcggtg gggattgatg tcgaggaggc gctgcgccaa cttgatcgtt
    16561 tacccgtttc aatgcactgc tggcagggcg atgatgtttc cggttttgaa aacccggaag
    16621 gttcgctgac cggggggatt caggccacag gcaattatcc gggcaaagcg cgtaatgcca
    16681 gtgagctacg tgccgatctg gaacaggcta tgcggctgat tccggggccg aaacggctta
    16741 atttacatgc catctatctg gaatcagata cgccagtctc gcgcgaccag atcaaaccag
    16801 agcacttcaa aaactgggtt gaatgggcga aagccaatca gctcggtctg gattttaacc
    16861 cctcctgctt ttcgcatccg ctaagcgccg atggctttac gctctcgcat cccgacgaca
    16921 gcattcgcca gttctggatt gatcactgca aagccagccg tcgcgtttcg gcctattttg
    16981 gcgagcaact cggcacacca tcggtgatga acatctggat cccggatggt atgaaagata
    17041 tcaccgttga ccgtctcgcc ccgcgtcagc gtctgctggc agcactggat gaggtgatca
    17101 gcaagaagct aaaccctgcg caccatatcg acgccgttga gagcaaattg tttggcattg
    17161 gcgcagagag ctacacggtt ggctccaatg agttttacat ggggtatgcc accagccgcc
    17221 agactgcgct gtgcctagac gccgggcact tccacccgac tgaagtgatt tccgacaaga
    17281 tttccgccgc catgctgtat gtgccgcagt tgctgctgca cgtcagccgt ccggttcgct
    17341 gggactgcga tcacgtagtg ctgctggatg atgaaaccca ggcaattgcc agtgagattg
    17401 tgcgtcacga tctgtttgac cgggtgcata tcggccttga cttcttcgat gcctctatca
    17461 accgcattgc cgcgtgggtc attggtacac gcaatatgaa aaaagccctg ctgcgtgcgt
    17521 tgctggaacc taccgctgag ctgcgcaagc tggaagcggc gggcgattac actgcgcgtc
    17581 tggcactgct ggaagagcag aaatcgttgc cgtggcaggc ggtctgggaa atgtattgcc
    17641 aacgtcacga tacgccagca ggtagcgaat ggctggagag cgtgcgggct tatgaaaaag
    17701 aaattttgag ccagcgtggg taaacactgc cggatgcggc gcgagcgcct tatccggcct
    17761 acgggtcggc aacagttgta ggcctaataa gacgcgacag cgtcgcatca ggcattgatt
    17821 gccggatgcg gcacgagtgc cttatccggc ctacaggtcg gcaatagttg taggcctgat
    17881 aagacgcgac agcgtcgcat caggcattga ttgccggatg cggcacgagc gccttatccg
    17941 gcctacgggt cgtgcatctg acaacaccga atttacagga acacagaaca tgcaaaacat
    18001 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg
    18061 ctgggacgaa cgcaacggcg gcaacctgac gctgcgccta gatgacgccg atatcgcgcc
    18121 ttataaagac aacgtccatg cacaaccgcg ctatatcccg ctcagccagc ccatgccttt
    18181 actggcaaat acaccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
    18241 cgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gatggcgcgg gctaccatat
    18301 tctctggggg ttaaccaacg aagccatccc gacatccgaa ctcccggcgc actttctctc
    18361 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
    18421 caccaacctg atcgccctca cctatgtact ggaaaacgac accgcggtct ttactcgcca
    18481 actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc
    18541 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
    18601 ttcgctggtg ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac
    18661 attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
    18721 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
    18781 cgttacacca ctagccagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
    18841 tgtcatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg
    18901 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    18961 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc
    19021 ggtagccagt actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
    19081 taacccggat aacagcccgg tgagtagcga gctgca
//
LOCUS       DAJTMK010000072        19099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTMK010000072 DAJTMK010000000
VERSION     DAJTMK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19099
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.073"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            41..970
                     /gene="aaeR"
                     /locus_tag="OO135_002145"
     CDS             41..970
                     /gene="aaeR"
                     /locus_tag="OO135_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5397984.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1098..2543)
                     /gene="tldD"
                     /locus_tag="OO135_002146"
     CDS             complement(1098..2543)
                     /gene="tldD"
                     /locus_tag="OO135_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5397985.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADTWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2699..6499)
                     /gene="yhdP"
                     /locus_tag="OO135_002147"
     CDS             complement(2699..6499)
                     /gene="yhdP"
                     /locus_tag="OO135_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5397986.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRGPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGDVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRDWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6567..8036)
                     /gene="rng"
                     /locus_tag="OO135_002148"
     CDS             complement(6567..8036)
                     /gene="rng"
                     /locus_tag="OO135_002148"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5397987.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8026..8619)
                     /gene="yhdE"
                     /locus_tag="OO135_002149"
     CDS             complement(8026..8619)
                     /gene="yhdE"
                     /locus_tag="OO135_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5397988.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8628..9116)
                     /gene="mreD"
                     /locus_tag="OO135_002150"
     CDS             complement(8628..9116)
                     /gene="mreD"
                     /locus_tag="OO135_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5397989.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9116..10201)
                     /gene="mreC"
                     /locus_tag="OO135_002151"
     CDS             complement(9116..10201)
                     /gene="mreC"
                     /locus_tag="OO135_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5397990.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRTLRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPHSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10267..11310)
                     /gene="mreB"
                     /locus_tag="OO135_002152"
     CDS             complement(10267..11310)
                     /gene="mreB"
                     /locus_tag="OO135_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5397991.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11615..13555)
                     /gene="csrD"
                     /locus_tag="OO135_002153"
     CDS             complement(11615..13555)
                     /gene="csrD"
                     /locus_tag="OO135_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5397992.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAVYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSIQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13707..14681
                     /locus_tag="OO135_002154"
     CDS             13707..14681
                     /locus_tag="OO135_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5397993.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15659..16129
                     /gene="accB"
                     /locus_tag="OO135_002155"
                     /gene_synonym="fabE"
     CDS             15659..16129
                     /gene="accB"
                     /locus_tag="OO135_002155"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5397994.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16140..17489
                     /gene="accC"
                     /locus_tag="OO135_002156"
     CDS             16140..17489
                     /gene="accC"
                     /locus_tag="OO135_002156"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5397995.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17581..18628)
                     /locus_tag="OO135_002157"
                     /pseudo
     CDS             complement(17581..18628)
                     /locus_tag="OO135_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18625..>19099)
                     /locus_tag="OO135_002158"
     CDS             complement(18625..>19099)
                     /locus_tag="OO135_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5397996.1"
                     /translation="FDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHST
                     PERAIAGFLEEGVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWI
                     ACRQLGFDVHRALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIQEAAQ"
BASE COUNT         4596 a         5273 c         4724 g         4506 t
ORIGIN      
        1 atattatgtg atctaaatca cttttaagtc agagtgaata atggaacgac taaaacgcat
       61 gtcggtgttt gccaaagtag ttgaatttgg ctcttttact gccgccgcca gacagctaca
      121 gatgagcgtt tcgtccatca gtcagacggt gtcaaaactg gaagatgagt tgcaggtaaa
      181 gctgttaaac cgtagcacac gcagcattgg cctgaccgaa gccggtagaa tttactacca
      241 gggctgccgt cgtatgcttc atgaagtgca ggatgttcat gagcaactgt atgccttcaa
      301 taacaccccc atcgggacgc tacgcattgg ctgttcttca actatggcac aaaatgttct
      361 cgccgggctg acagccaaaa tgctgaaaga atacccaggt ttgagcgtca atctggttac
      421 cggaattcca gcccccgacc tgattgccga cggtctggat gtggtgatcc gcgtcggcgc
      481 gttgcaggat tccagcctgt tttcccgccg tcttggcgcg atgccaatgg tggtgtgcgc
      541 cgcgaaaagc tatctcacac aatacggcat accggaaaaa cccgccgatt taagtagcca
      601 ttcatggctt gaatacagcg tgcggcccga caatgaattt gaactgattg caccggaagg
      661 gatctcgact cgcctgatcc cgcaagggag atttgtgact aatgatccga tgacgctggt
      721 gcgctggcta acggcgggtg ccgggatcgc ctacgtgccg ctgatgtggg tgatcaacga
      781 gatcaatcgt ggggagctgg agatcctgct gccgcgttat cagtcagatc cacgcccggt
      841 ttatgcgtta tataccgaaa aagataagct gccgctgaag gtacaggtcg tgatcaactc
      901 gctgacggat tattttgttg aggtcggtaa attgtttcag gagatgcacg ggcgcgggaa
      961 agagaagtaa ttcatattgt actgttacgt tctacaaacc tgtgccaacg ggttcccctc
     1021 accctaaccc cagaggagcg aggggaccgt tcgtgcacgt agaaagatta attatccttc
     1081 tgaaaataat gaaattatta cgcagtaccg ccaacagtca ggttatcaac tttcagcgtt
     1141 ggctggccca cgccaaccgg caaactttgc ccttctttac cgcagacacc cacgccgtta
     1201 tccagtttca ggtcgttgcc aaccatcgaa atctgctgca tggtttcgat accggaacca
     1261 atcaacgttg cgcctctcac cggcttcgtt actttaccgt tttcaatcag atatgcttct
     1321 gaagtggaga aaacgaattt gccggaggtg atatccacct gaccgccacc aaagttcggt
     1381 gcatagatac cgtactcaac ggattcaata atttcctgcg gggtcgattt acccggcagc
     1441 atataggtgt tggtcatacg cggcatgggc agatgggcgt aggattcacg gcgaccgttg
     1501 ccagtcggcg tcatccccat caaacgcgcg ttgagtttat cctgcatgta gcctttcaga
     1561 atgccgttct caatcagcac gttgtactgg cctggcgtac cttcgtcatc aatcgccacc
     1621 gaaccacggc gatcgaccat cgtgccgtca tcaaccacgg tacacagttc tgaagccacc
     1681 agctccccga cctgcccact aaatactgaa gtgccgcggc ggttgaagtc gccttccaga
     1741 ccgtgaccta ccgcttcatg caacagcacg cccggccaac ctgcgccaag tactaccggc
     1801 atggtgcccg ctggtgcagc aacggcagaa agattgacca gcgccatacg cactgcttct
     1861 tttgcccatg tatccgcacg gacttcgccg tcaagatcgg caaggaagaa ctcataacca
     1921 aaacgaccgc caccgccact ggcaccgcgt tcgcgtttgc catcttcttc gaccagaacg
     1981 ctcacggaaa gacgcaccag cggacggaca tccgccgcta gcgtgccgtc ggtggccgca
     2041 accagaatta attcatagac accactgagg ctggcagtca cttcctgtac gcgcttgtcc
     2101 gcttcgcgag caaccttatc gacgcgacgc aggatatcca gcttctcttc acggctcatg
     2161 ctttgcagcg gatctaccga ggtatacaac gggctatgcc ctaccgcgcc cagcgtctgt
     2221 actttgccat caccactatc acggacgatg gtgcgcgccg cttgcgcact ctgttccagc
     2281 gccagcaggc tgatttggtc agcgtaagca aatccggttt tttcaccgct gattgcacgc
     2341 acgccaacgc cctgatcgat gttgtaagaa ccatctttaa taatgcggtc ttctaaaacc
     2401 caggattcgt gatagctcga ctgaaaatag agatcgccat aatcaaggcg acgttcggcc
     2461 agttgaccga ggatcgcgaa caagtcctga tgtttcaggc cgttcgccgc tagcaattgt
     2521 tcacttacca ggttaagact catcgttttt gctactcgtt agttactgca gtagaggatt
     2581 ttttacggct gccggatgcg gcgtgaacgc cctatccgac ctacggttat gttctggctc
     2641 gttggccttt ggcaacgatt atcatatgag attggggcaa ttacgcgccc tcgtcaaatc
     2701 attgcgcttt ttctttacgc ggttggcgca acacttcgtt gatttgcgga tcgtccagcg
     2761 gacccgaaat gtgatagcgc aaaatggaga ctttgctcca cagcggcccc agcactttac
     2821 tggctgcaaa tactgccgcg ccaacaatgg ggttaaccgc aaaagccgca gccacgccca
     2881 ccgtcgcaga aatctctggt gcgacaaccg cttccatatt caggtcgcga cgtaccagat
     2941 ttaccgaccc tttcatggcg atatccgcct ccaggccatc caccagcgtg tcgtcggtgt
     3001 gcataacgcc gtctttaatc cacgcggtgc tgcgaatgga gtcaaaatag aacccttcgc
     3061 caaaagtgtc tctgaaatca aaacgcagct tacgcatcag ggcatcaacg ctcaataagc
     3121 gcagcaattg cccggcatgt ccggtattga tttcggtaat ttcgccttta cccagttgag
     3181 tatgaatgat gccattcaac gtcgcctcat ctggttgcca gggtgctttg cgccagtgta
     3241 aatcgtaatc cacattaaat gacgactggc gtatgggcgt cgtgacacca aaaaattctg
     3301 cggcggcatc aattttctgc ccgcgcagtt ttcctttcag cgaggtacgt tcattccccg
     3361 gattattaac ccattcacca tcggcagtaa gccgcgagaa accagtatca atcagtccat
     3421 tggtcagcgt taacgtatcg ccagaaatgg tgagatcact gtcaatacga ccgaattttt
     3481 gcccccagaa ccagcactct gtgcatcgta tttgtgcgtc cggccagtca cggaagttaa
     3541 tgcgctccgt tgtcgggaaa ggtgatgacg gtgttgaatc accacgagtt ttcgccacgc
     3601 tcgggttgta ataaagatat ttgatattcg ccagccacgg cgcgttatta cgcatcgcca
     3661 gcgtggcatt gatttcacgc ccctgcgcct caaccagggt gccatttgcc gtcggttgcg
     3721 aaacaatact caggttattc cattgctgat ttcccagtga caacataggc gtacgtaacg
     3781 ttatgtgttg tgggaaactc gctgcaccac cgacactctc cgcagcgcct ttctggaaca
     3841 gggccagcca ctcggcacca ttcatcggcg gcatattgag ttcaacgcca ctttgttccg
     3901 gcaacggcgg gagcgtttta ctgtctgccg cccaaatagc acgatcgagc gtcagctttt
     3961 gaccaagcaa ccagcggcta ttgaaatggt tatccgcacc agcctgtccg gttaattcaa
     4021 agctgctgag attgccatca accttaacgt ttaccggcag tggttcaccc gcaggtttgg
     4081 ctaacggtga aggtaagtga ctgctcacat tattcagatc gccgttcagc tctacgttat
     4141 aggtcgcgcc agcatgataa ggcagatcaa tgcccacttt accatcccac gccacgctgc
     4201 cactcaatgc ttcgttcacc gctgcaggca gaacgccggt tttcgccggt tgccagttac
     4261 cgttgaggtt taccgcaacc tggtaggctt ttgccccttc tttggtggaa aaatccacgt
     4321 tcaacggctg attaaaccag cttgctgtca gtggttcact ttgcagatcg ccattgataa
     4381 agctgaattt accgctcaaa tttttcaggg tgctgtcgag tggtttgata aacagactgt
     4441 tattacgcag cgtcacttca cctttcgcgg ttaccagttc gccgttcagc gggatatcaa
     4501 gatgtaagcg agcattcaca tcgccgtcga gctggagttc ttgcagggtc gcacccagag
     4561 aatctttcag cggtgtctca tcaaagtaag ggccaacggc tttacccgga cctttaatgt
     4621 cagcgtcaat cagcagtttt tctttcgagt agtcagggat cactgcggtg agattactcg
     4681 cgcgcacgtc gcccagatta acgtcatcgg ttttcatcca taaaccgtcg ttaataaagt
     4741 ccagttcaat atcaaggtta gttaatgcag gccagtccgg ctggaaggca aacttcgcgt
     4801 tacgcagcgg caccagcact tcaaactgac cttcgttgtg tttatagggg aagagttgcg
     4861 gattgccacc ataaaccagc gtcgcgttat ccgcttcacc gccctgaatc gcgccactta
     4921 agtaatcaac taggtcttta cccatcaagt tttccgggaa atagcgccag gcttgtgaac
     4981 catcatcggt actgatgcca gccagaatac ccagccaggg ttcatcgtta gcaggttgca
     5041 ggtaacgaaa accgccgcgc gcatggacgg ctttggcttt aacgtcaata ttacgcccat
     5101 ccagctggaa acctttgtca ttgttcagcc agcttatagt tgcctggccg tcggcgattt
     5161 ccagtggccc acggaatacc gtttcgtaag gcatctttgc ctgcttcatc gacgcggtaa
     5221 gcaaaccatt ttcaacgctg ccggaaagcg tcccggagaa gtgttccgca cccggtaata
     5281 atttccattg cttccaggcc agatcgctcc acgatgcctg aaaacgggtc ttgtctgccg
     5341 cctgaagcgg gatatccagc gccagagtgt taatcttgcc gctcggttgt gtggagcgcc
     5401 aaacatcacc cagtgcaggt gaaagtttcg cggccagcgg gcgtatgccc tccaggcctg
     5461 ccagctccag attactggcg cgaatccgga gttcgtcact gcgtttattg tctttgccgc
     5521 caacgtcctg ttccggtatc caggccagcg tcaatgctcc gctcggccag ggtttgccgt
     5581 ccatcgtgat ccgtgtatcg ggaatagaga actgccagcc cggattttca cgcgtaatat
     5641 gcgcggtcag attatccacc gacagcgtat gcgtttgctt ctcgcccaac cagctggcac
     5701 cgccctgttt cagccagacg tcaccgccgg ttacatcgcc tttgtcgatc gtcatccagc
     5761 cttcaaggga gaactgtgcc gtttccagag caatattgtc ctgcatccat ttaccgagcc
     5821 acggcttcag gtcgatgtca tccgcctgta gccagacgcg accattgctt aacaacccct
     5881 catcatcgcg caaatccatg cgcacctgca tcacgccgtg ctgtccggta aggctggaga
     5941 ggcttaccag gccttccgca cggtgtcgac gtggatcgtt cagccaggtg agttgtggga
     6001 tcgccagctc ggcgcgctga ccggatggcg tcagaaaact gacttcactg tcgcggagat
     6061 cgaaatggtc aaattgacga agaaacagat cgctgatgtg actggcttcc aggctatcat
     6121 taccaccacc gctggtgata ggagtgttgg tgcgaaagcg cagctgccag aaagtgaggt
     6181 cgcgaaactg ccagcgcata tgtaacaagc tctgccagac atccagcgcc agagtaacgc
     6241 gtttaaccga aaattcgccg ccatctttta gttctgcacg gatgtcgtgt gcttcaagcg
     6301 tcgggccaaa attctgccag ctggctgaga gctgactggc ttctaccggc atgccagtcg
     6361 cggattctat tttgttgagg atttccggac gccaggcgtc aagatgcggt aaagcaatac
     6421 gcaggccgct aaccagcagg gctgcgatca caacgagcgc ggctccagta agcagtaaaa
     6481 tccccggcaa tcgcctcacc cgtcactcct tgtctgctaa aaatgtgact caaaaaacct
     6541 ttgccggatg gcggcccagc atctgtttac atcattacga cgtcaaactg ctcctggtta
     6601 tagagcggtt caatttgtac tttaacctgt ttgccaacga aaatttccac ttccgccagc
     6661 gagtgtgact cttcgccttt caaggcttca gctactgccg gagaagcata gaccaggaaa
     6721 cggtcggagt cgtaagcatg gtggacacga acaatctcgc gcatgatttc atagcatacc
     6781 gtttccacgg ttttcaccgt tccgcgaccg tggcaggttg ggcattcgtt acacagtacg
     6841 tgctcaatgc tttcgcgggt gcgtttacgc gtcatctcca ccagccccag cgccgaaaaa
     6901 ccattaacgc tggttttcac ccggtctttg ctcaacgcct gctccagcga gtgcagcacg
     6961 cggcggcggt gatcttcatt attcatatcg atgaaatcaa taatgataat cccacccaga
     7021 ttacgcaacc gtaactggcg agcgatagcc tgcgtcgctt caatattggt attgaaaatg
     7081 gtgtcgtcca gattgcgatg accgacaaac gctccggtat tgatgtccac ggtggtcatc
     7141 gcttcggtct ggtcgataat cagataacca ccggatttca gttctacttt gcgttccagc
     7201 gctcgctgga tttcgttttc gacatcaaag agatcgaaaa tcggctggcg tccggtgtaa
     7261 tgctccagct tgctggtcat ctcgggaatg tactccgagg taaactccag caatgcttcg
     7321 taagtcaggc gtgagtcaac gcgaatgcgg tccagttcgg cgtcggcgaa atcacgcaga
     7381 acacgctgcg ccagcgccag ttcgccgtac agctgataac gggtctgcgg gcgtttttta
     7441 cgctccataa ctttggtcca gacgcgtttc agataagcgg catcggaggc cagttcagcc
     7501 tcgccaaccc cttccgctgc ggtacggatg ataaacccgc cctgctcgtc gcaatactct
     7561 gcgaccactt ttttcaggcg ttcacgttct gattcgcttt caatacgttg ggaaacccca
     7621 acgtgagaag cccctggcat aaacaccaga tagcgagaag gcagcgtgat atcggtggtc
     7681 aggcgcgcac ctttagtgcc aagcggatct ttcaccacct gcaccatcag atcttgcccc
     7741 tgacgaacca gttccgagat gtcgcgcacc gtgaattgct tttgttcttc acccgccaca
     7801 cattcggtgt gcggcatgat atcggatgca tgaagaaacg cggctttatc cagcccaata
     7861 tctacaaaag ccgcctgcat acccggaagt acacgactta cacgaccctt gtagatattg
     7921 cctactattc cgcgtcgcgc ctcacgttca atatgaattt cctgcagaat accgccatca
     7981 atatacgcca ctcgcgtttc cgaaggcgtt acgtttacta acaattcagc cgtcatgttt
     8041 atcccttttc tcacgcagtg cgttaaaatt acttaataat tcatacgttt caaccagcgg
     8101 taagccgact acggcgtgat agctgccatt tatcttcctg acaaaacagc ctcccagccc
     8161 ctgaataccg tatgcacctg ctttatctaa cggttcaccg ctggcgacat agcccgcgat
     8221 gtcttcgtct gttaacgttc tgaaagtcac atcggtgacc accaggcaat cgagaatgtg
     8281 ctggctgtcg gccaacacca ctgccgtcat cacctgatgg gtctgacccg ataatttgcg
     8341 caacatctgc gccgcatgct ctgcgtcgcg cggtttctcc agcacttctc cattcaggat
     8401 aacgatagta tccgcaccca gcaccgggag atcctgcgcc gtttgcgcga cacccgcctg
     8461 tgctttctcg cgtgccagac gcacaacata ctgctgcgcg ctctcctgcg gctgacgctg
     8521 ctcctcaatg cccgtaacaa tacgttcaaa ggtcacgcca agttgcgcaa gtaactcctg
     8581 acgacgcgga gaaccggaag ctaaatacag agaagtcata gaaaccttta ttgcactgca
     8641 aactgctgac ggactttacg catcagcaag aaaatccacg gccagagcac cccattgact
     8701 acactactcc agaacacttc cggtctgaaa gagacgttaa tcactaaaaa ctctgcccag
     8761 aaaacaataa tatccaccac cagcgaaagc aacatgacga ctagcgcctg ctgccataat
     8821 gcgaggttgc ggaaaagctg gtatttcagc gccaccaggt aagcaatgat gctcatcgcc
     8881 aatacgcgta cgccaagcgt cgagccgctg atcagatcca gtatggcacc catcacaaaa
     8941 cctgtgccca catttacgcg atggggcaag gccaggatcc aatacaacaa gataagtaac
     9001 acccagtttg gccggaaaac aatcaggtta tccggccagg gcatgatttg cagcaacagc
     9061 gcaatgagga aagagagcca gattacccaa cgtccctggc tacgatagct cgccactatt
     9121 gccctcccgg cgcacgcgca gggggttgag caccactttg cggcggcgta gccctttgtg
     9181 gagagcgatt agcagcaggc gcagtagctg catttcctgt cgtcggttgc tgcggagtcg
     9241 gctgagcgat ccccgttgcc ggttcaggta actttggccc catcgcgtct ggcgaaggca
     9301 atacctgcgg catcatctgc atcagacgtt cattagcaac acgatgcacc tcttccggcg
     9361 tcatcgggtt agcgccgtta cgatccgcgc cccacagcag cagcagataa cgcaaacgtt
     9421 gcagtcctgc agtcggacgc gcctgaatca cagtataagc gcgctgggta tcgagtttta
     9481 cggaagagac aaccgcgacc ggatagcctt ccgggaaacg accgcccaga ccggaggtca
     9541 ccagcacatc accaacacga atatccgtat tcgctggcag atgctcaagc tgcaaatcat
     9601 ccgtacaacc gttaccggct gcaattacgc ggatatcgtt gcgcagcacc tggattggca
     9661 gcgcgtgggt cgcatcacaa atcagcagca cgcgactggt cagtttagcg acggccacca
     9721 cctgaccaac aacacctttg tcgctgatga ccggctggcc ttcataaacg ccattaacgc
     9781 tacctttatc gataacaact tgatcgctat gaggatcgtt aaccgtggag ataacctgag
     9841 tcaccatttt ctgctcatcc tgacgcagcg gggaacccag cagctcgcgc agacgcgcgt
     9901 tctcctgttt gtattgtcca agcatcagca gttcactgtt tttcagcaac agttcctgac
     9961 gtaacgtccg gttttcaagt tctagttggt cacgcgaggc cagcgtctgc gatacgccat
    10021 ccagcaattc acgaggagca ttggaaacaa agtagaaagg actgacggcg gtatccatat
    10081 aagtacggat ttgactgaac gtccccaggc gactgtcggc aataataatg ccgagcgcca
    10141 ccagcaccgc cagaataagg cgaatctgta gcgacgggcc acggctaaaa attggcttca
    10201 taagttatgc gtattctcgt atcagaccag gcagggtaaa cagacatttc ccctgcctgc
    10261 atccgattac tcttcgctga acaggtcgcc gccgtgcatg tcgatcattt ccagcgcttt
    10321 gccgccaccg cgcgccacac aggtcagcgg gtcttcagca acaacgactg gaatgccggt
    10381 ttcttccatt aacaaacggt caaggttacg cagcagtgcg ccaccaccgg tgagcaccat
    10441 gccgcgctcg gagatgtcgg aagccagttc cggcgggcac tgttccagtg caaccattac
    10501 cgcgctcaca ataccggtca gcggttcctg cagtgcttcg aggatttcgt tggagttcag
    10561 ggtaaaaccg cgtggaacac cttctgccag gttacggcca cgaacttcga tttcacggac
    10621 ttcatcgccc gggtaagccg aaccgatttc gtgcttgata cgttctgcgg tggcttcacc
    10681 gatcagagaa ccgtaattac gacgcacata gttgatgata gcttcgtcga aacgatcacc
    10741 accaatgcgc acggaagagg agtaaaccac gccgttcaag gagataacag caacttcagt
    10801 ggtaccacca ccgatatcaa ccaccataga accggtcgct tcagaaaccg gcaggccagc
    10861 accaattgcg gcagccatcg gttcttcaat caggaagact tcacgggcac cagcgccctg
    10921 cgcggattca cgaattgcgc ggcgttcaac ctgggtcgcg ccaaccggta cacaaaccag
    10981 aacgcgcggg cttggacgca taaagctgtt gctgtgcact tgtttgatga agtgctggag
    11041 cattttttca gtcacgaaga agtcggcgat aacgccgtct ttcattgggc gaatggcagc
    11101 aatattgccc ggcgtacggc ccagcatctg cttcgcgtca tgacctactg cagctacgct
    11161 tttcggtgaa ccggcacgat cctgacgaat ggccaccacg gaaggctcat tcaatacgat
    11221 gccttgtcct tttacataaa tgagggtatt cgcagtaccc aggtcaatgg acaagtcatt
    11281 ggaaaacatg ccacgaaatt ttttcaacat actaagggat aatcctgaaa gctggggcgg
    11341 aaaagaaaat ccgcttactt taccaaccac acgcagcagc gacaaggcgc aaaaatcatc
    11401 tgctacggtg aaaattagtg cagttcgttt cctttgttac aaatctctgc ctgagtccag
    11461 aaaggcttaa tgcatcagca gcattcctcg cctgtttgca accgcgtaag gtcattcatc
    11521 tgcatatgtg ctgcaacaat ctggcgagca gacaagcaca ctcccatgag acgcagcgcg
    11581 cgttattcta cgtgaaaacg gattaaacgg caggttaaac cgagtatctt tgtgaatatt
    11641 ttttcacgtt agtatcaagt ggctgtgagg acgcgaaaaa atccccttgc ccgcctgtaa
    11701 caccgcgctg aatcagggtc tgccactcgc ttcgcgaacg cacaccggtg gcgtaaacct
    11761 ggatgctggt cccggagcag gcttccacca ggctttgaac cagcagctgg ttctccgttc
    11821 gcttctcaat gtttctgacc agccccggat ggagcttgag taactcaaca ttaagttctt
    11881 tgatccaact ggtgcttacc agcgtcaaac cagcctggtt gacggctacc cgtaccccta
    11941 aagcattcac taaacgaata acaggttgta aacgactgat atgttgacct acatcggcct
    12001 ctgcaagttc aataattatg cgttttcgtt gtgatttttc acattgcatt aacgtatcgc
    12061 gcagccaacg ctgaaaacgc gggcgaatca gcgactcaac ggtaacctga atcgccagat
    12121 tttcctctgg ccagtaacgc aatagtggaa taagacggct gatttgcaga cggtcatact
    12181 cttccgataa gccaaactgc aagaccatcg gcatatactc cgccgagcta acctcttcat
    12241 taccatcgaa gatgcggcac atgagttcgc gatgatgaac ctgaccttcg cgagtaaccg
    12301 ccggtttttg ataaaggcgc gggccgccgc gactgagcat ttgctcgata agcgtacgcc
    12361 agcgaacatt accgcgtcct ttttcaggca acgagtcatc gtaaacagcc cagctattgc
    12421 cgccctgcaa tcccgcatta cgcgtggcag actctgcatg ttccattacc tgctcggtat
    12481 cctgaccact acgccaggcg cagataccaa tgtggatcat atcgtcgcga tcgagcattt
    12541 tattgttcgg caaggtatcg acggctttga ttaactgacc ggcgatgctc tctgcctctt
    12601 ttaacgtccg gtgcggtaac agcgcagcaa aatcactgcg gtggtaacgc gccagcagtg
    12661 cgccagggta gcgcatcata aatgtcgaca gcagattcgt cagagtgaag aactgttctt
    12721 caacctggct gtgcccccaa gtatcgctca acatattgaa atccggcaga cgaatcatca
    12781 tcacgatccc gtgggtacct actttctcct gatcttccag taacgttgct aactgattat
    12841 cgaaaaagag tcggttattg aggccggttt tcacgtcctg ggcggcataa gagcggatca
    12901 gcgtatcaag acggctgtgt tgttcgcgtg cgttctgaat ttcacgaagc agcgtatcca
    12961 gcgcactgct ggttctgggc ggccattcat agatggttcc caacacatta gagccacgct
    13021 caccgtttaa gatacgagta gcccgggttt ccagcaattc ttgcccggca agttgccgtt
    13081 gtaaccagcg taccgccaag aagagcataa caatgataaa gctaatcgcc cccgtgagcg
    13141 gcgcggtggt catcaacgaa tggaaatagt tgcccatcgg atcctgataa accagacgca
    13201 gcgacatccc cggatgcttt atcaacggaa cgctcagttc acgaaacaga tcgctggagc
    13261 caaccggacg ataactacca tttctggcca gggtataaac ctgtttatca ccatggagca
    13321 ggtctacacg aacgatatct gccgacatca ttaattcggt aatttgtggc cttaatgtgc
    13381 tgaagtcatt cgacacaagg tgggtatcaa tcgccgtcgc caccgcctga acgcgatgac
    13441 taaacttata ctgaattgcg ttgtagaaac ttagcgaaca gcccagcaaa gtcacaaaaa
    13501 ttgttaaccc ggtgagcagc gtaacaaagg ccgaaaattt cgtcgttaat ctcatccttg
    13561 tgttaactcc gatagtgagg aagcgggcat actagcaaat cagatttatc tcgcaattta
    13621 ttgcgcttca tcggctttgc ttttccatta gcgagtataa tcttcagaaa ttattttcca
    13681 atccatcatg cacatgagga ccacttatgc aggcgttact tttagaacag caggacggca
    13741 aaactctcgc atcagtacag actctggacg aaagtcgcct gccggagggc gatgtcacgg
    13801 tcgatgttca ctggtcgagc ctgaactata aagatgcgct ggcgattacc ggtaagggaa
    13861 aaatcatccg taattttccg atgattcctg ggatcgattt tgccggaact gtacgcacca
    13921 gcgaagatcc gcgttttcat gccggtcagg aggtgttact cactggctgg ggcgttggtg
    13981 aaaaccactg gggtgggctg gcggagcagg cgcgagtgaa aggtgactgg ctggttgcca
    14041 tgccgcaagg gctggacgcg cgtaaagcaa taattatcgg tactgccggt tttaccgcca
    14101 tgctgtgtgt gatggcgctg gaagatgccg gtgttcgccc gcaggacggg gagattgtcg
    14161 tgacgggtgc cagtggtggc gtcggcagta ccgccgtggc gctgctgcat aagttgggtt
    14221 atcaggtcgt tgccgtttcc ggtcgcgaaa gtacccatga atatctgaaa agtttaggtg
    14281 ccagccgtat tctccctcgt gatgagtttg ccgaatcccg tcctctggaa aaacaagtct
    14341 gggctggggc aattgacact gttggcgaca aagtgctggc aaaagtgctg gcgcaaatga
    14401 attacggcgg ctgcgtggcg gcctgtggtc tggcgggtgg ttttactctg ccaaccacgg
    14461 tcatgccatt tattctgcgt aatgtccgtt tgcaaggggt ggattcagta atgacgccac
    14521 cagaacgccg cgcacaagcc tggcagcgac tggtcgccga tttaccggaa tcattctata
    14581 cccaggcggc aaaagagata tctctgtcag aggcaccgaa ctttgccgag gccatcatta
    14641 ataaccagat ccagggtcgc acgctggtga aggttaacta accatttagc aaggaataat
    14701 aagagaggga actcattttg acatcattat tcaatcggtt aacgggaaaa gcggttagct
    14761 ggacagcttt cgtcgaacac cttggtcagg aagttgtaca acatcatcca aactggaaag
    14821 tcatgatttc gactgaccac aaattgatgc gcattgatac tcctctaaac agccattatt
    14881 gatacgcctc cgtcgcctgt taggtttatg gtgctttgcc tgggcgacac tacgcttaac
    14941 ctgttactga tttctggtac catggggtga ataatctgat tttatttgac tacaaattaa
    15001 ccactcgaac ctatttaatg ctgagaattg tcagtcggtt gattttgcgt gctttagcat
    15061 tcacatctat ccagacgatg cagtgaaaat tgggtaatcc ccagcaaccg ctgcgtaatg
    15121 tcgtctatct tgtcgcgatc ctggcatccc tacattattt gtggtctgtg aagattatct
    15181 cattgcagcc cctcatcttc gcagggctgg ctttacagct tttcacctta cgttataaga
    15241 agttccgtcg atgatggcgc taatttcgtg aattgtgcgg cttgttgcaa attacacggt
    15301 gttgaaggtt atttacatgt tagctgttga ttatcttccc tgataagacc agtatttagc
    15361 tgccaattgc tacgaaatcg ttataatgtg cgacctcgtc ctccctgacg cagtttttgc
    15421 gctgcggaaa aggtgacatt ggcgcaacga aggtatattt tgttttttgc cggaggatag
    15481 cagcagatcg ctgcacaatg tccgtcaagt ctaacattga tactctgggg caaaatagac
    15541 cggcgtcccg gcctgctgga atttatcgct atgcatacag ctgtcggggc atacgcttta
    15601 cagacggcgg tgaaacgcct gtcacaatca cactaaacaa agagtacgga acccactcat
    15661 ggatattcgt aagattaaaa aactgatcga gctggttgaa gaatcaggca tctccgaact
    15721 ggaaatttct gaaggcgaag agtcagtacg cattagccgt gcagctcctg ccgcaagttt
    15781 ccctgtgatg caacaagctt acgctgcacc aatgatgcag cagccagctc aatctaacgc
    15841 agccgctccg gcgaccgttc cttccatgga agcgccagca gcagcggaaa tcagtggtca
    15901 catcgtacgc tccccgatgg ttggtacttt ctaccgcacc ccaagcccgg acgcaaaagc
    15961 gttcatcgaa gtgggtcaga aagtcaacgt gggcgatacc ctgtgcatcg ttgaagccat
    16021 gaaaatgatg aaccagatcg aagcggacaa atccggtacc gtgaaagcaa ttctggtcga
    16081 aagtggacaa ccggtagaat ttgacgagcc gctggtcgtc atcgagtaac gaggcgaaca
    16141 tgctggataa aattgttatt gccaaccgcg gcgagattgc attgcgtatt cttcgtgcct
    16201 gtaaagaact gggcatcaag actgtcgctg tgcactccag cgcggatcgc gatctaaaac
    16261 acgtattact ggcagatgaa acggtctgta ttggccctgc tccgtcagta aaaagttatc
    16321 tgaacatccc ggcaatcatc agcgccgctg aaatcaccgg cgcagtagca atccatccgg
    16381 gttacggctt cctctccgag aacgccaact ttgccgagca ggttgaacgc tccggcttta
    16441 tcttcattgg cccgaaagca gaaaccattc gcctgatggg cgacaaagta tccgcaatcg
    16501 cggcgatgaa aaaagcgggc gtcccttgcg taccgggttc tgacggcccg ctgggcgacg
    16561 atatggataa aaaccgtgcc attgctaaac gcattggtta tccggtgatt atcaaagcct
    16621 ccggcggcgg cggtggtcgc ggtatgcgcg tagtgcgcgg cgacgctgag ctggcacaat
    16681 ccatctccat gacccgtgcg gaagcgaaag ctgctttcag caacgatatg gtttacatgg
    16741 agaaatacct ggaaaatcct cgccacgtcg agattcaggt actggctgac ggtcagggca
    16801 acgctatcta tctggcggaa cgtgactgct ccatgcagcg ccgccaccag aaagtggtcg
    16861 aagaagcacc agcaccgggc attaccccgg aactacgtcg ctacatcggc gaacgttgcg
    16921 ctaaagcgtg tgttgatatc ggctatcgcg gtgcaggtac tttcgagttc ctgttcgaaa
    16981 acggcgagtt ctatttcatc gaaatgaaca cccgtattca ggtagaacac ccggttacag
    17041 aaatgatcac cggcgttgac ctgatcaaag aacagctgcg tatcgctgcc ggtcaaccgc
    17101 tgtcgatcaa gcaagaagaa gttcacgttc gcggccatgc ggtggaatgt cgtatcaacg
    17161 ccgaagatcc gaacaccttc ctgccaagcc caggcaaaat cacccgtttc cacgcacctg
    17221 gcggttttgg agtgcgttgg gagtctcata tctacgcggg ctacaccgta ccgccgtact
    17281 atgactcaat gatcggtaag ctgatttgct acggtgaaaa ccgtgacgtg gcgattgctc
    17341 gcatgaagaa tgcgctgcag gagctgatca tcgacggtat caaaaccaac gttgatctgc
    17401 agatccgcat catgaatgac gagaacttcc agcatggtgg cactaacatc cactatctgg
    17461 agaaaaaact cggtcttcag gaaaaataat ttgtgacgcg cggtgttttc atcgcgctta
    17521 gcaaagaacg taatcaacgc cgggcctgtt aacgctgccc ggcgttttta ttgccgttag
    17581 ctaacggtag cctgcaattt ccgctttcaa cgcgctcatt tcagcatcgc catcaacaac
    17641 tcccagctca aagcagtatt tctttgtttc ccctggctca ataaagggca gacgcccctg
    17701 cgctcgttct ttatcacgac cgtcaggaaa actattactg acctccagtc ccataacata
    17761 ctcacccgcg cctaaatttt tccactggat aaagttgggt agctgcatgg tgtcgaattt
    17821 ctcgtaaatt ccccacccca gttcggcgtt caccagagca actttactca tcccctgact
    17881 gtcgctattc aacgtgagat agtaaacctg ctcgtcaaaa ccgggctgtg gggtacaaat
    17941 ctccagatga ctggttaacc cttccgccgc atgcggagtt cgtggcgtta tgcttttagc
    18001 cgacagcaag atttctggcc tgtggcgata ataaaggcca gccgtaatta aagtgataaa
    18061 gaatttgcag cggctggcgg gtgaaaccat gattagtcac cttgtcttca atgcgcagca
    18121 cattttcgcc ataacggcag cgaatggtcc tttccagcgt cagattttcg ccgaacagtc
    18181 gcgtttcccg tactttgcca ttgatgacaa attcaatgcc gtcgtcggtc cgttctgtgc
    18241 gataccccac ctcttctgct ggagtcgcag acaaacgccc gtgtaacccc agcgtttctc
    18301 cttcatcctc acaaggcgtg ccaatgtagc taagtccgca ggtagtcagt aaccccgcgt
    18361 aaaagctacg taaaaatccg ttgccctgag actcaaaaaa agcgggggcg acaatgccgt
    18421 tgggggtgat aaaagaaagc gccgtgtcct tgtagctggc ccatgcaata tccagcgcgc
    18481 gatcttttac caccgtaaat gccagtcccg caccagtacg gacatcaaac gcttctgtac
    18541 cctttgctct gccactacgt aactcatagt gtttaattcc ggcaacctgc gagagatcgc
    18601 caatccacga ccaaatttcg tttttcattg tgctgcttcc tgaatggaca tgtcatggcg
    18661 ctggatgaac gcttcgattt ccgcaaggcg agacgttccc tccattgggc cacgtctggt
    18721 taccgccagc gcaccgcagg cattggcata ttgcagcgct cgatggacgt caaaccctag
    18781 ctggcggcag gcaatccacg cgccaccaaa gcagtctccc gcaccggtcg ggtcaacttc
    18841 ttccaccgga tagctctcaa catgaaactg ctcattcgcg gagtagtaac tggcaccttg
    18901 attgccgcgc ttcacaatca cctctttaac accttcttca agaaaacctg cgattgcacg
    18961 ctccggcgtc gaatgtggcg acaagagcaa cacttcgccc tcgctgggca tatagatatc
    19021 cgttaactca agaacaaagt ggagggcgtc acgcatttct ggaatatcga gcatctcttt
    19081 acgaatatta gggtcgaaa
//
LOCUS       DAJTMK010000073        18909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTMK010000073 DAJTMK010000000
VERSION     DAJTMK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.074"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OO135_002159"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OO135_002159"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5397997.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OO135_002160"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OO135_002160"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5397998.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OO135_002161"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OO135_002161"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5397999.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OO135_002162"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OO135_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5398000.1"
                     /translation="MTQLTIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OO135_002163"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OO135_002163"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5398001.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OO135_002164"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OO135_002164"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5398002.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="OO135_002165"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="OO135_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5398003.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OO135_002166"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OO135_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5398004.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OO135_002167"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OO135_002167"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5398005.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHS
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OO135_002168"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OO135_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5398006.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OO135_002169"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OO135_002169"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5398007.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="OO135_002170"
     CDS             complement(15664..16701)
                     /locus_tag="OO135_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5398008.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLKILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="OO135_002171"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="OO135_002171"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5398009.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNCGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..>18909
                     /locus_tag="OO135_002172"
     CDS             17758..>18909
                     /locus_tag="OO135_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR5398010.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTSNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPM"
BASE COUNT         4825 a         5008 c         4600 g         4476 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcatcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaaggac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcaccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgtgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaactc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggtgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cacggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtt gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccatt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttatcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc agcatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgctg
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgaa tggaggaaat cgccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aatcaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtagacttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc gaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 tttcagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacggt cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgagtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg gtgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaactg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttatcca atacatatat tgagatttaa ctcataatta cttcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgttt gtttttacca
    18241 aattcaaaaa accaaacaag taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttg caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta
    18781 ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag
    18841 ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat
    18901 cctcccatg
//
LOCUS       DAJTMK010000074        18744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTMK010000074 DAJTMK010000000
VERSION     DAJTMK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18744
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.075"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..733
                     /gene="rihB"
                     /locus_tag="OO135_002173"
     CDS             <1..733
                     /gene="rihB"
                     /locus_tag="OO135_002173"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5398011.1"
                     /translation="IMRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMA
                     SDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPE
                     AARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFEN
                     YGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTK
                     VGITIDTDWFWGLVEECVRGYIKTH"
     gene            833..2083
                     /locus_tag="OO135_002174"
     CDS             833..2083
                     /locus_tag="OO135_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5398012.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGGDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2139..3227)
                     /locus_tag="OO135_002175"
     CDS             complement(2139..3227)
                     /locus_tag="OO135_002175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5398013.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3230..4087)
                     /gene="nfo"
                     /locus_tag="OO135_002176"
     CDS             complement(3230..4087)
                     /gene="nfo"
                     /locus_tag="OO135_002176"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5398014.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4161..5210)
                     /locus_tag="OO135_002177"
     CDS             complement(4161..5210)
                     /locus_tag="OO135_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5398015.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5310..6191
                     /locus_tag="OO135_002178"
     CDS             5310..6191
                     /locus_tag="OO135_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5398016.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            6397..7866
                     /gene="lysP"
                     /locus_tag="OO135_002179"
     CDS             6397..7866
                     /gene="lysP"
                     /locus_tag="OO135_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5398017.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8159..10139
                     /gene="cirA"
                     /locus_tag="OO135_002180"
                     /pseudo
     CDS             8159..10139
                     /gene="cirA"
                     /locus_tag="OO135_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10171..11007)
                     /gene="yeiG"
                     /locus_tag="OO135_002181"
     CDS             complement(10171..11007)
                     /gene="yeiG"
                     /locus_tag="OO135_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5398018.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYYLGQGAGF
                     YLNATQPPWATHYRMYDYLRNELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11265..11933
                     /gene="folE"
                     /locus_tag="OO135_002182"
     CDS             11265..11933
                     /gene="folE"
                     /locus_tag="OO135_002182"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5398019.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11950..13107
                     /locus_tag="OO135_002183"
     CDS             11950..13107
                     /locus_tag="OO135_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5398020.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13249..14289
                     /gene="galS"
                     /locus_tag="OO135_002184"
     CDS             13249..14289
                     /gene="galS"
                     /locus_tag="OO135_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5398021.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSTHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14569..15567
                     /gene="mglB"
                     /locus_tag="OO135_002185"
     CDS             14569..15567
                     /gene="mglB"
                     /locus_tag="OO135_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5398022.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15628..17148
                     /gene="mglA"
                     /locus_tag="OO135_002186"
     CDS             15628..17148
                     /gene="mglA"
                     /locus_tag="OO135_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5398023.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLMGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17164..18174
                     /gene="mglC"
                     /locus_tag="OO135_002187"
     CDS             17164..18174
                     /gene="mglC"
                     /locus_tag="OO135_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5398024.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18417..>18744)
                     /locus_tag="OO135_002188"
     CDS             complement(18417..>18744)
                     /locus_tag="OO135_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5398025.1"
                     /translation="LADQGFDSLQEMVGLANNNIVPAEDLDRSYIVYPRINLDKCVGC
                     GRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGCIDLGEVKFKKGEK
                     EHPVTL"
BASE COUNT         4477 a         4606 c         4979 g         4682 t
ORIGIN      
        1 cattatgcgt aaacaaatcg ttgccgataa tattcacggt gaaaccggac tggatggtcc
       61 ggtattcgag tcgctgaccc gccaggcaga aagtacccac gcggtgaaat acatcattga
      121 taccctgatg gcaagcgatg gcgatattac tctggtgccg gttggtccgc tttcaaatat
      181 cgcggtggca atgcgtatgc aacctgcgat cctgcccaaa atccgtgaaa ttgtgctaat
      241 gggcggcgct tacggtacag gcaacttcac gccatctgcc gagttcaaca tctttgctga
      301 tccggaagca gcccgcgtgg tgttcacctc cggcgttcca ttagtgatga tgggcctcga
      361 tctcaccaac cagactgttt gcaccccgga cgtgattgca cggatggaaa gggcaggcgg
      421 ccctgccgga gagctgttca gcgacatcat gaacttcact ctcaaaacgc agttcgaaaa
      481 ctacggcctg gctggcggcc cggtgcacga cgccacctgc atcggttatc tgattaaccc
      541 tgatggcatt aaaacccagg agatgtacgt cgaagtggac gtcaacagtg gcccttgcta
      601 tgggcgtacc gtctgcgacg agctgggcgt tcttggcaag cccgctaata ccaaagtcgg
      661 catcactatt gataccgact ggttctgggg cttagtcgaa gagtgcgtgc gcggctacat
      721 caaaacccat taagtgttga taacaagccg ggtcagtatc gcaagatcca agcccggcta
      781 tttctgatgg gcagaaagcc gtcgcccaat atgacatgaa gagataataa ctatggatgt
      841 catgagaagt gttctgggaa tggtggtatt gctgacgatt gcgtttttgc tgtcagtaaa
      901 caagaagaag atcagcctgc gtaccgttgg cgcggcgtta gtgttacagg tcgtgattgg
      961 cgggattatg ctttggttac cggcagggcg ttgggtcgct gaaaaagtcg cttttggcgt
     1021 gcataaagtg atggcgtaca gcgatgcggg tagcgcattt atcttcggtt cgctggtcgg
     1081 gccgaaaatg gacacgctgt ttgatggcgc aggatttatc tttggtttca gggtattacc
     1141 ggcaattatc ttcgtcactg cactggtgag tattctctac tacatcggtg tgatggggat
     1201 tttaattcgc attctcggcg gtatattcca gaaagcatta aatatcagca agattgagtc
     1261 attcgtcgcg gtcaccacca ttttcctcgg gcaaaacgaa ataccggcga tcgtcaaacc
     1321 ctttatcgat catctgaatc gcaatgaatt atttacagcg atttgtagtg gcatggcctc
     1381 gattgctggt tcgacaatga ttggttacgc cgccctgggc gtacctgtgg aatatttgct
     1441 ggcggcatcg ttaatggcga tcccaggcgg gatcttgttt gcccgcctgt taagcccggc
     1501 tacggaatct tcgcaggttt cctttaataa cctctctttc accgaaacac cgccaaaaag
     1561 cattattgaa gctgctgcga caggggcaat gaccgggctg aaaatcgccg ccggtgtagc
     1621 gacagttgtt atggcatttg tcgccatcat tgcgttaatt aatggtatta tcggcggcgt
     1681 tggcggctgg tttggttttg cacatgcctc gctggagtcc attttaggtt acctgttggc
     1741 cccattggcg tgggtgatgg ggggtgactg gagtgatgca aatcttgccg ggagtttgat
     1801 tgggcagaag ctggcgatca atgaatttgt cgcttatctc aatttctcgc catatctgca
     1861 aacgggtggc actctggatg ctaaaaccgt ggcgattatt tctttcgcgt tgtgcggttt
     1921 cgctaacttt ggttctatcg gggtggtggt gggggcgttt tctgcggttg cgccacaccg
     1981 tgcgccggaa atcgcccaac ttggtttacg cgcgctggcg gcggcgacac tttctaacct
     2041 gatgagtgct actattgcag gattctttat tggtttagcg tagtgcgcaa tttatgagcc
     2101 ggataagatg cggtcaagcg tcgaatccgg caacattgtt acaccattgg cactaatgaa
     2161 agcgcgttat cggcagacag ggtagggttg tttaagctgc cgctggcgcg cgaaattgcc
     2221 gcgcaggcta ccgcaaaacg ggcgctgtcg cggaaactgt atccttcaag aaagctatat
     2281 accaggcccg ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt atgcgctggc
     2341 gcggtcagca aaaattgttc tccatccttt tcgctgcaat aaactgactc atcgggcaaa
     2401 taaacaaaca gttgctgaac accttgctga tgcaatgcat tcactgcggc attacggtca
     2461 gcatcgctgg tgatcgcctg tccccataaa atttccagct ccggtaaagt gggtttcagg
     2521 gtgtgaatat gcgccagcca gtgtttgatt ttgcccgctt tgaattctga aacggtatcg
     2581 acaaacaccg ggatttcatc agcgagggta aagacccatt ccagcgcctc ggctgtcagg
     2641 ttgcaatcag ccagtaccac gcccgcatga cgaagtaaat cgcgcgaccc gttcagtagt
     2701 tgcggtgtca actgttccag cagatgggta tcgttaatcg ccagcacggt ttgatcgtct
     2761 cgattggcaa ttgccagata cgtcgatgtg ctttgaccat gcaaacgaac gcagccggag
     2821 acattcacgc cggcacggcg cgtttcttcc aggagcattt cgccataaaa gtcatcgcca
     2881 atcactgaaa gcaaatggac gtcacggcct aacagcgcca gattgtgggc gatgttgcgt
     2941 cccacgccgc ccgccgagca atgaattgta ccgggatgag aagccgcttg cgggtaacgg
     3001 atatccgcca tcccgcgaat atccatattg attgtcccca ccactacgca gtattcctgc
     3061 tcggtgagaa tgtaaccttt gcctttaatc cgccctttgc gcattaaatc cataatatgc
     3121 gccgcaacgc gcgaacgact gatttgcagc atgtccgcaa tttcgttctg ctgaatcagc
     3181 gggttacgcc gtaaaattgc aaggatctcc ttttcccggt tattcatctt caggctaccg
     3241 ctttttcagt ttgttgcgct ttcagccagg cgatctcttc tgcccagata tccgggttga
     3301 tggtttcaag gatcagcgga atgccgtcga aacggtcgtc ctgcatgatc cagcggaacg
     3361 catcatgacc gatattgcct tcaccgaggc tatgatggcg gtcaacgcgg ctgccaaagg
     3421 tgcttttcgc atcgttaagg tgcatcccgc gcagatactt aaagccaaca atacgggcaa
     3481 aatccgcgaa cgttttctcg cattcggctg gagtacgcaa atcatacccg gcagcgaaag
     3541 catggcaggt atcaatgcag acgccgacac gggatttatc ttccacgccg tcgataatcg
     3601 ccgcgagatg ttcgaattta aaccctaagt tactgccctg accggcggtg ttttcaatca
     3661 ccgctgttac accttgagtt ttatccagcg caatgttgat ggattcggca atacgcgcaa
     3721 ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg gtggaagttg agcaaagaaa
     3781 gccccagttg ttcgcaacgc tgcatttcat ctataaaggc atcgcgcgat ttttccagag
     3841 cttcagtgac cggatgtccg aggttaatca gatagctgtc gtggggaaga atttgcgccg
     3901 atgtgtagtg atatttttca caggccgctt tgaattcatc gatggtttgc gtcgtgagcg
     3961 gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc aaacgcggtt gcgtcgattt
     4021 cggcggcgcg aattgcggca tttgccagac cgccagcagc actaacgtgc gctccaatgt
     4081 atttcatgcg aggactcctg ttaaacccgc tggaggaaaa cggtaatgat agcgggttaa
     4141 caggagcaag atgtagtggt ttatgcgatg acgctttgaa tcacatagtt aatcgcacca
     4201 ccaccaacaa tcagccaggc aaacagtacc agtgccatca gcagaggttt cgccccggct
     4261 tttttcagcg cgctgacgtg agtggtcaga cccagcgccg ccatcgccat tgccagcagg
     4321 aaggtatcca gcgttaccag catgttcacc acgctctgcg gtaacaggtg gaacgagtta
     4381 aagatggcaa cgacgatgaa caagatggca aaccacggaa tagtgatttt gcttttctcg
     4441 ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca gcaggatgag gaacggagcc
     4501 agcatcatca cgcgcagcat tttggaaata actgctgcgt tttccgcatc cgggctgatg
     4561 gcatgacctg ccgccaccac ctgcgccact tcgtgcacag tagaaccaat gtagataccg
     4621 aaagtttccg gactaaacca ttgagacatc agcggatata tcgccgggta gaggaaaatc
     4681 gcgacggtcc cgaagataac aacggttgca acagccacgg ttactttact ggcttccgct
     4741 ttcactaccg gctcagtcgc cagtaccgcg gcagcaccac agatactgct accggcaccg
     4801 atcaaccagc tggtgtgctt atccagacca aacactttct gccccaggaa gcaagccagc
     4861 aggaaggtac tggacagcgt caacacgtca atgatgatcc cactgatacc gacatcggca
     4921 atttgcgaga acgtcagacg gaagccataa agaatgatac ccagacgtaa taaatattgc
     4981 ttggcaaaca gcacaccacc gtcacagctt ttccagatgt gcggatagat ggtgttgcct
     5041 aaaaccatcc ccaacaagat tgcgagggtg agggcactaa acccggcacc cgcaaccgcg
     5101 ggaatggaac caccccacag ggcgaccccg gtgataactg cactcagggc taaccccgga
     5161 ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga tattcgtcat aaccctctcc
     5221 ttataaccgg gcataaggtt acgactaact ggtttaaaaa taaaattgat tatatattta
     5281 taattaatct ttataagtgg taagcgacta tgcacatcac cctccggcag ttggaagttt
     5341 ttgcagaagt attgaaaagt ggatcaacca cccaggcgtc ggtgatgctg gcgttgtcgc
     5401 aatcagcagt gagcgcagcc ttgaccgacc tggaagggca gcttggcgtg caactgtttg
     5461 atcgcgtggg gaaaagactg gttgttaatg aacacgggcg gctgctctat ccgcgtgcgt
     5521 tggcattgct tgaacaggcg gttgaaatcg aacaactgtt tcgcgaagac aacggcgcga
     5581 ttcgtatcta tgccagtagt accatcggta attacattct gcctgcagtt atcgcccgtt
     5641 atcgccatga ttatccgcag ttgccgattg aacttagcgt tgggaacagc caggacgtga
     5701 tgcaagcggt gctggatttc cgcgttgata ttggctttat tgaaggaccg tgccacagca
     5761 ctgaaatcat ttctgaaccg tggctggaag acgagctggt ggttttcgcc gcgccgactt
     5821 cgccgttggc tcgtggtccg gtcaccttag aacagctggc cgctgcgccg tggatcctgc
     5881 gtgaacgcgg ttccggcacg cgggagattg tcgattatct attgttgtca catttaccga
     5941 agtttgagat ggcgatggaa ttaggtaact ccgaggcaat caaacatgcg gtgcgtcatg
     6001 ggttgggaat tagttgcctg tcgcgacggg tgattgaaga tcaattgcag gcaggcacat
     6061 taagtgaagt tgcggtccct ctgccgcgcc tgatgcgtac gttgtggcgt atacatcatc
     6121 ggcaaaaaca cctttccaac gcgctacggc gctttctgga ctattgcgat cccgcgaatg
     6181 taccgcgtta agttgctgta caagaacatg ctggtgctgt gtcgatttcg tgacgcagcg
     6241 ccctcagcat gcattcgcca gaaaagagat tggctgcttt acttataatc cctgggcgat
     6301 catgaaggtg tcttataacc gtgtatttct gccggaagga ttgccaatcg tctgctacaa
     6361 tcgcgccatc atttttaaga tggatagcat ttttgtatgg tttccgaaac taaaaccaca
     6421 gaagcgccgg gcttacgccg tgaattaaag gcgcgtcacc tgacgatgat tgccattggc
     6481 ggttccatcg gtacaggtct ttttgttgcc tctggcgcaa cgatttctca ggcaggtccg
     6541 ggcggggcat tgctctcgta tatgctgatt ggcctgatgg tttacttcct gatgaccagt
     6601 ctcggtgaac tggctgcata tatgccggtt tccggttcgt ttgccactta cggtcagaac
     6661 tatgttgaag aaggctttgg cttcgcgctg ggctggaact actggtacaa ctgggcggtg
     6721 actatcgccg ttgacctggt tgcagctcag ctggtcatga gctggtggtt cccggataca
     6781 ccgggctgga tctggagtgc gttgttcctc ggcgttatct tcctgctgaa ctacatctca
     6841 gttcgtggct ttggtgaagc ggaatactgg ttctcactga tcaaagtcac gacagttatt
     6901 gtctttatca tcgttggcgt gctgatgatt atcggtatct tcaaaggcgc gcagcctgcg
     6961 ggctggagca actggacaat cggcgaagcg ccgtttgctg gtggttttgc ggcgatgatc
     7021 ggcgtagcta tgattgtcgg cttctctttc cagggaaccg agctgatcgg tattgctgca
     7081 ggcgagtccg aagatccggc gaaaaacatt ccacgcgcgg tacgtcaggt gttctggcga
     7141 atcctgttgt tctatgtgtt cgcgatcctg attatcagcc tgattattcc gtacaccgat
     7201 ccgagcctgc tgcgtaacga tgttaaagac atcagcgtta gtccgttcac cctggtgttc
     7261 cagcacgcgg gtctgctctc tgcggcggcg gtgatgaacg cagttattct gacggcggtg
     7321 ctgtcagccg gtaactccgg tatgtatgcg tctactcgta tgctgtacac cctagcgtgt
     7381 gacggtaaag cgccgcgcat tttcgctaaa ctgtcgcgtg gtggcgtgcc gcgtaatgcg
     7441 ctgtatgcga cgacggtgat tgccggtctg tgcttcctga cctccatgtt tggcaaccag
     7501 acggtatacc tgtggctgct gaacacctcc gggatgacgg gttttatcgc ctggctgggg
     7561 attgccatta gccactatcg cttccgtcgc ggttacgtat tgcagggaca cgacattaac
     7621 gatctgccgt accgttcagg tttcttccca ctggggccga tcttcgcatt cattctgtgt
     7681 ctgattatca ctttgggcca gaactacgaa gcgttcctga aagataccat tgactggggc
     7741 ggcgtagcgg caacgtatat tggtatcccg ctgttcctga ttatttggtt cggctacaag
     7801 ctgattaaag gaactcactt cgtacgctac agcgaaatga agttcccgca gaacgataag
     7861 aaataagttt cctcccttcc ttgctaagcc ctctcaaccg agagggcttt ttcaattcca
     7921 tttccctgac aaatcatgcg gatataaaat ttaacattta gattgataat tgttatcgtt
     7981 tgcattatcg ttacgccgca atcaaaaaag gctgacaaat cagaggctgt tccggctttc
     8041 tgggatgatc acctgcatac aaataagtcc accgcgatgc tgccgtacgc aaggggacgt
     8101 gaagaagatg tgagcgataa cccattttat tttcgtagtt acctcatgga gatatggaat
     8161 gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg tccgctattt cttgtgcatg
     8221 gcctgtgtta gcggtcgatg atgatggcga aacgatggtt gtcactgcat cttccgttga
     8281 acaaaacctc aaagatgcac ccgccagtat cagcgtcatt acccaggaag acctgcagcg
     8341 aaaaccggta cagaatctga aggatgtcct caaagaagtg cttggcgtac aactgacgaa
     8401 cgaaggggat aaccgtaagg gcgttagtat tcgtggtctg gacagcagct acaccctgat
     8461 tctcgtcgac ggtaaacgcg ttaactcccg caatgccgtc ttccgccaca atgatttcga
     8521 tctgaactgg atcccggtcg attccatcga acgtattgaa gtggttcgtg gcccgatgtc
     8581 gtcgctatac ggctccgatg cgctcggcgg tgtagtgaat atcatcacca aaaaaatcgg
     8641 tcagaaatgg tcgggtaccg ttaccgtcga taccaccatt caggaacatc gcgatcgcgg
     8701 tgatacctat aacggtcagt tctttaccag tggaccatta attgatggtg tgctgggaat
     8761 gaaagcttac ggcagcctgg caaaacgtga aaaggatgac ccgcaaaact caacgaccac
     8821 cgataccgga gaaacgccgc gtattgaagg attctccagc cgcgacggca atgtcgaatt
     8881 tgcctggaca ccgaatcaaa atcacgattt tactgccgga tacggtttcg accgtcagga
     8941 actcgctgga caaaaaccgc ctggaacgcc agaactactc cgtcagccat aatgggcgtt
     9001 gggattacgg caccagcgaa ctgaaatact acggtgagaa agtcgagaac aaaaaccctg
     9061 gcaacagcag ccggataact tccgaaagca atacggtcga cggcaaatac acgttgccgc
     9121 tgacggcgat taatcagttt ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg
     9181 atgcggtgaa cctgaccggg ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt
     9241 ttgtggaaga tgaatggcgg atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg
     9301 acgatcacga aacctacggt gaacactgga gtccgcgtgc ctacctggtt tataacgcca
     9361 ccgacaccgt aacggtgaaa gggggctggg cgacggcatt taaagcacct tctctgttgc
     9421 aacttagccc tgactggacg agcaattcct gccgtggcgc atgtaagatt gtgggtagcc
     9481 cggatctgaa accagaaacc agcgaaagtt gggagctggg gctttactac atgggtgaag
     9541 aaggctggct ggaaggggtt gaatccagcg ttaccgtttt ccgtaacgat gtgaaagatc
     9601 gtatcagcat cagccgtacg tctgacgtca acgctgcacc gggctaccaa aaccttgttg
     9661 gttttgagac gggcgctaac ggacggcgca taccggtatt tagctactac aacgttaaca
     9721 aagctcgtat tcagggcgtg gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac
     9781 tgtcgatcaa ctacacctac aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc
     9841 tatccgatct gccgttccat actgctaacg gtacgctgga ctggaaaccg ctggcgctgg
     9901 aagactggtc attctatgtt tctggtcact atatcgggca gaaacgcgcc gacagcgcga
     9961 cggctaaaac accgggcggt tataccatct ggaataccgg cgcggcctgg caggtgacta
    10021 aagacgtcaa actgcgcgca ggcgtgctga accctggcga caaggatctc agtcgtgacg
    10081 actacagcta taacgaagac ggacgtcgtt actttatggc agtggattat cgcttctgat
    10141 gagaagatgc ccggcgaacc gggcggactt tcacttcagt aaatactgcg catggaagcg
    10201 caggtgatcc tctataaaag aggcgatgaa gtagtaactg tgatcatatc ccggctgaat
    10261 acgcagcgtc atcggccacg ctttctggcg cgcggcttct gccagtaccg caggttgcaa
    10321 ctggtcggca agaaactgat cattatcgcc ctgatcgata agcgtcggga tcgcatcctg
    10381 cgcgttactg gcatacatca gcgcgcaact gtcccattcc agccatgcat ttttgtcctc
    10441 acctaaatag ctgctaaacg ctttgattcc ccacgggacg ctgcacggat tcacaattgg
    10501 cgcaaaggcc gaaacgctgg tgtatttacc cggatttttc agcgccataa tcagcgcacc
    10561 gtgaccaccc attgagtgac cgctaatggc gcagcggtcg ctgacattaa attgcgactg
    10621 aaccagcgcc ggtaattcat tgcgcagata atcatacatc cggtaatgcg tcgcccacgg
    10681 cggttgcgtg gcattaagat aaaagcctgc gccctggcct aaatagtagc catcgtcgtt
    10741 ggcaaccttt tcgccgcgcg ggctggtgtc tggcatcacc agtacaatcc ccagttctgc
    10801 cgccacgcgc tgggcacccg ccttggtggt gaagttctcg tcattgcagg ttaatccgga
    10861 aagccagtac agcactggtg gcggggtgtg atcacgaggg ggtgggagaa agatactgaa
    10921 cgtcatcggg cagtttaagg tactggagtc gtgtcgccag cgttgctgcc agccttcaaa
    10981 acagcggtgc tcttcgagca tttccatgcg tggctccttg ttgtgttgtt tgcaatttct
    11041 tcatcatacc gtttattcaa tgtgctgtga gtaactttca cttccgtatt tgcataacga
    11101 tgttttaaca tctgctgatg aaaggcagcg gcaattacaa taattatcgc tgtgaatact
    11161 ggattatgtg cgccgcctca cgcacaataa tcaggctgta aatcagctta ataactttgc
    11221 ccccacgcag ggcggaggcg tcacacctgc aggagaaatc ataaatgcca tcactcagta
    11281 aagaagcggc cctggttcat gaagcgttag ttgcgcgagg actggaaaca ccgctgcgcc
    11341 cgcccgtgca tgaaatggat aacgaaacgc gcaaaagcct tattgctggt catatgaccg
    11401 aaatcatgca gctgctgaat ctcgacctgg ctgatgacag tttgatggaa acgccgcatc
    11461 gcatcgctaa aatgtatgtc gatgaaattt tctccggtct ggattacgcc aacttcccga
    11521 aaatcaccct cattgaaaac aaaatgaagg tcgatgaaat ggtcaccgtg cgcgatatca
    11581 ctctgaccag tacctgtgaa caccattttg ttaccatcga tggcaaagcg acggtggctt
    11641 atatcccgaa agattcggtg atcggtctgt caaaaattaa ccgtatcgtg cagttctttg
    11701 cccagcgtcc gcaggtgcag gaacgtctga cgcagcaaat tcttattgcg ctacaaacgc
    11761 tgctgggcac caataacgtg gctgtctcca ttgatgcggt gcattactgc gtgaaagcgc
    11821 gtggcatccg cgatgcaacc agtgccacaa caacgacctc tcttggtgga ttgttcaaat
    11881 ccagtcagaa tacgcgccac gagtttctgc gcgctgtgcg tcatcacaac tgattaaaag
    11941 gcaggaacca tggagcgcaa cgtaacgctc gattttgttc gcggcgtcgc cattctgggg
    12001 atcctgctat taaacatcag cgcctttggg ctaccaaagg cggcttatct caatcccgcc
    12061 tggtacggcg ctattacgcc gcaggatgca tggacctggg catttctcga tctcatcggc
    12121 caggtgaaat tcctcacgct ttttgcgctg ctgtttggtg cgggcctgca aatgttgctg
    12181 ccccgtggca gacgctggat ccagtcgcgg ttaacgctgt tagtcttgct gggttttatt
    12241 cacggtttat tgttctggga cggcgatatt ctgctggctt acgggctggt gggcttaatc
    12301 tgctggcggc tggtgcgcga tgcgccatcg gtaaaaagcc tgtttaatac aggcgtcatg
    12361 ctttatctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    12421 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    12481 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    12541 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    12601 agcggctggt tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    12661 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    12721 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    12781 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    12841 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    12901 atttgtacca tgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    12961 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    13021 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    13081 acggcaatat ctaaaacatc aagataacga tctggatcac attcgttaac aaaacggctg
    13141 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    13201 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    13261 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    13321 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    13381 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    13441 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctagtc
    13501 gctcagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    13561 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    13621 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    13681 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    13741 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    13801 actcacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    13861 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    13921 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctat atttgcttat
    13981 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    14041 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    14101 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    14161 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    14221 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    14281 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    14341 tgattgcacc gttttaacgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    14401 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    14461 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccctgcc
    14521 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    14581 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    14641 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    14701 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    14761 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    14821 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    14881 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    14941 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    15001 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    15061 cagtccgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    15121 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    15181 atgtgggata ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    15241 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    15301 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgctct gccagaagcg
    15361 ctggcgctgg tgaaatccgg tgcactggcg ggcaccgtac tgaacgatgc taacaaccag
    15421 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gtaaaggtgc ggctgatggc
    15481 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    15541 aacctggctg aattcagcaa gaaataagac tgattgggcg cagtctatta ctgcgccctt
    15601 attgtatgac caacaaggta aaattatatg gtcagctcaa cgactccgtc ctccggggaa
    15661 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    15721 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggcgcagga
    15781 aaatcaacat tattaaaatg cttgtttggt atttatcaaa aagactccgg caccatttta
    15841 ttccagggta aagagatcga tttccattcg gccaaagaag cactggaaaa tggtatttcg
    15901 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggacaa catgtggctt
    15961 gggcgatacc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    16021 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatct
    16081 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    16141 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    16201 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaggaa
    16261 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    16321 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaac
    16381 cagcgtttcc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaacctg
    16441 acgtcactgc gccagccgtc gatccgtgat gtctcgtttg atctgcataa aggggagatc
    16501 ctcggtattg ccgggctgat gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    16561 attcgcgaga aatcggccgg caccattacg ttgcacggca aaaagatcaa taaccataat
    16621 gccaatgaag ccataaacca cggatttgct ctggtaactg aggagcgccg ctcaacggga
    16681 atttatgcct atctggatat cggttttaac tcgttaattt ccaatattcg caactacaaa
    16741 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    16801 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    16861 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    16921 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    16981 ctggcgaaga aaggcaaagg gattattatt atctcctctg aaatgcctga gttgttaggg
    17041 ataacagacc gtattctggt catgagcaac ggtctcgttt ccggaattgt cgacacaaaa
    17101 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    17161 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    17221 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    17281 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    17341 gcagggttaa ttgtcaccca ggggaccgac ctttctgcgg ggcgtcaagt agggctggcg
    17401 gcagtggtgg cagcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    17461 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    17521 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    17581 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    17641 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    17701 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    17761 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    17821 gcaaaagtat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggt
    17881 gtgttctatg ccttcggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    17941 ctcggcttta tgtatgaact ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    18001 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    18061 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    18121 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgaaatgat
    18181 tgtggtccgc attcggtcat aaacgcacat aacatcatga tctgtgtggt tttgtaggcc
    18241 ggataaggcg ttcacgccgc atccggcgac aggagccaaa tgccggatgc aacgctggcg
    18301 cgttttatcc ggcctacaat gagctcaaca tactgatttg tatggttttg taggccggat
    18361 aaggcgttca agccgcatcc ggcgacagga accaaatgcc ggatgcgtct ctggttttac
    18421 aacgttaccg ggtgttcttt ctctcccttc ttaaacttca cttctccgag atcaatacaa
    18481 cccaccgggc agacgtggcc acaaagcaga caacccacac atttctcggt attacaatgc
    18541 ggtgtgcggg ttttctcgct ccattccatc gcctggtgac cgccgtcgta acaggaaata
    18601 taacagcgtc cacagccaac acatttatca agattgatac ggggatagac aatataactg
    18661 cggtctaaat cttccgccgg gacaatattg ttattcgcca gacctaccat ctcctgcaac
    18721 gaatcaaatc cctgatcggc gaga
//
LOCUS       DAJTMK010000075        18735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTMK010000075 DAJTMK010000000
VERSION     DAJTMK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.076"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..894
                     /locus_tag="OO135_002189"
     CDS             <1..894
                     /locus_tag="OO135_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398026.1"
                     /translation="DWFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFI
                     TIGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRT
                     MWGMMLGFSAINYTAWLYLAWLPGYLQTAYYLDLKSTGLMAAIPFLFGAAGMLVNGYV
                     TDWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGT
                     SCWGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVT
                     AAGALAYIFLVRQPINDPRKD"
     gene            complement(943..2598)
                     /gene="tsr"
                     /locus_tag="OO135_002190"
     CDS             complement(943..2598)
                     /gene="tsr"
                     /locus_tag="OO135_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5398027.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2717..3163)
                     /gene="hpaR"
                     /locus_tag="OO135_002191"
     CDS             complement(2717..3163)
                     /gene="hpaR"
                     /locus_tag="OO135_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5398028.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3435..4713
                     /locus_tag="OO135_002192"
                     /pseudo
     CDS             3435..4713
                     /locus_tag="OO135_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
     gene            4710..6176
                     /gene="hpaE"
                     /locus_tag="OO135_002193"
     CDS             4710..6176
                     /gene="hpaE"
                     /locus_tag="OO135_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5398029.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGMKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6178..7029
                     /gene="hpaD"
                     /locus_tag="OO135_002194"
     CDS             6178..7029
                     /gene="hpaD"
                     /locus_tag="OO135_002194"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5398030.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7039..7419
                     /locus_tag="OO135_002195"
     CDS             7039..7419
                     /locus_tag="OO135_002195"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5398031.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7530..8333
                     /gene="hpaH"
                     /locus_tag="OO135_002196"
     CDS             7530..8333
                     /gene="hpaH"
                     /locus_tag="OO135_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5398032.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8344..9132
                     /gene="hpaI"
                     /locus_tag="OO135_002197"
     CDS             8344..9132
                     /gene="hpaI"
                     /locus_tag="OO135_002197"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5398033.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9307..10683
                     /gene="hpaX"
                     /locus_tag="OO135_002198"
     CDS             9307..10683
                     /gene="hpaX"
                     /locus_tag="OO135_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5398034.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFSQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10693..11579
                     /gene="hpaA"
                     /locus_tag="OO135_002199"
                     /pseudo
     CDS             10693..11579
                     /gene="hpaA"
                     /locus_tag="OO135_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11830..13392
                     /gene="hpaB"
                     /locus_tag="OO135_002200"
     CDS             11830..13392
                     /gene="hpaB"
                     /locus_tag="OO135_002200"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR5398035.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13410..13922
                     /locus_tag="OO135_002201"
     CDS             13410..13922
                     /locus_tag="OO135_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5398036.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14315..16465
                     /gene="btsT"
                     /locus_tag="OO135_002202"
     CDS             14315..16465
                     /gene="btsT"
                     /locus_tag="OO135_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5398037.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGMIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16596..16799
                     /locus_tag="OO135_002203"
     CDS             16596..16799
                     /locus_tag="OO135_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5398038.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16810..17766
                     /gene="yjiA"
                     /locus_tag="OO135_002204"
     CDS             16810..17766
                     /gene="yjiA"
                     /locus_tag="OO135_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5398039.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17844..>18735)
                     /locus_tag="OO135_002205"
     CDS             complement(17844..>18735)
                     /locus_tag="OO135_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR5398040.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         4282 a         5184 c         4989 g         4280 t
ORIGIN      
        1 gactggttca acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc
       61 attggcgttg ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc
      121 gggatgttta ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc
      181 tatcgcaacc gcgagcacgt agaactgact gccgttgagc aagcttatct caatgcaggt
      241 agcgtcaatg cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac
      301 cgtacaatgt ggggaatgat gctcggattc agtgccatca actacactgc gtggctgtat
      361 ctggcctggc ttcctggtta cctgcaaaca gcctattacc tggatttaaa aagcacaggg
      421 ttgatggcgg ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt
      481 accgactggc tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt
      541 gccgggatgt tctgttctgc cgcctttacg ctgatagtac cacaagcgac aacatccatg
      601 acggcggttc tgctgattgg catggcactg ttctgtattc actttgccgg aacatcctgc
      661 tggggcttga tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag
      721 aactttgcca gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat
      781 accacccact cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca
      841 ctggcgtaca tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctcct
      901 tatgcccgat aacattttgc ttatcgggca ttttcatggc gattaaaatg tttcccagtt
      961 ctcctcgcta tctgccacgg ccattttacg cggcgcagct ggcgtcacgg tttttaccac
     1021 agccgatgtt tcacgctgct gttgctgaat ccggaacact gccactgctt cggtcaggcg
     1081 actggcctgc tcttccagcg cggcggcggc agcggcagac tcttccacca gcgctgcgtt
     1141 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
     1201 ctgctcatca gaagcagaag caatttcgcc cataatgtcc gtcacgcggg tcacggcgct
     1261 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
     1321 ccccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tgcgctgagc
     1381 cagattacgc acttctcccg cgaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc
     1441 agcctcaacc gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac
     1501 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
     1561 gttatctacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct
     1621 ggcctggcgc gcattttcgg cgttctgctt caccgttgcg gtcagttgct ccatgctggc
     1681 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc
     1741 ggtggcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
     1801 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
     1861 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
     1921 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
     1981 gaccgccagt acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc
     2041 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
     2101 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
     2161 gcctgcacct aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
     2221 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc
     2281 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
     2341 ggttgaaccg ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag
     2401 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
     2461 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc
     2521 gcctgatgtc agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat
     2581 cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
     2641 aaagcaaaaa ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt
     2701 atcagatact aaaaagttat tcattatcgc ccgggatggc ttcttgtcgg gaattgccca
     2761 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
     2821 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
     2881 cctctttggt tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
     2941 ctaaaccgtc gcgttccatc cgcgtgagaa tcccggtcag gcttgggcgc aaaatgcagg
     3001 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
     3061 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg
     3121 cttctcgcgc ctggagcaac gcaatggtta gtgagtcgtg cattatcttt cccctgaaaa
     3181 attcaaaatc attaatatag aaacagttaa tcgtcacgtt accgggactg cgctgcccct
     3241 gcaattcctg attttatcaa aattgatgat attttattaa cttactgata tttaatattt
     3301 tattatcttt aaatgtaaat ggtttgttaa ttagatcaca tttacatcac ttacttttgc
     3361 caaactacgc aatcgggaat aaaagtaatc attaacatat taatgattaa gatgatcgaa
     3421 tccgaggagt ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgctgccaac
     3481 ttgatgcatg gcaggaagcg ttccagcaat ccccctacaa agccccgcct aaaactgcgg
     3541 tctggtttat taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac
     3601 agggtgaaaa ggtactgagc ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga
     3661 aagtacgtga agaagatgcg gcagagtaca tcgccggata tgcgctggct aacgacgtca
     3721 gcctgccgga agagagcttt taccgcccgg caatcaaagc aaaatgtcgt gatggattct
     3781 gccccattgg cgaaaccgtg gctctcagca atgtcgataa tctgaccata tataccgaga
     3841 tcaacgggcg tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcgcagt
     3901 tgctgagcgc cctgagcgaa tttgccacgc tgaatccagg cgacgccatt ctgctcggca
     3961 cgccacaggc gcgcgtggaa atacagccag gcgagcgcgt tcgtgttctc gcagaaggtt
     4021 tcccgccgct ggaaaatccg gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc
     4081 caacgcagcc acacccgcac ggcacgctgt ttgccctcgg cctgaactac gccgaccacg
     4141 ccagcaaact ggaatttaag ccaccggaag aaccgctggt gttcctgaaa gcgccaaata
     4201 ccctcactgg cgataaccag acctccgtgc gtccaaacaa tattgaatac atgcactatg
     4261 aagcggagct ggtggtagtt attggcaaac gtcagcgaag ccgatgccat ggattatgtc
     4321 gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa ctactaccgc
     4381 cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac catcgtgccg
     4441 aaagaggcga tcccggaccc gcataatctg ccccttcgca ccttcgtcaa cggcgagtta
     4501 cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat cgcctactta
     4561 agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc caaaggctta
     4621 tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg ccgcctggtg
     4681 aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg atcaacggca
     4741 aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt gaagtgctgg
     4801 cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca gcgaaagagg
     4861 cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg cgccgtctgg
     4921 gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg gacaccggcc
     4981 tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac tttgaatttt
     5041 tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac aagatgctca
     5101 actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg aacgtaccgt
     5161 ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc gcggtactga
     5221 aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg ctggaagccg
     5281 gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca ggggatgcgc
     5341 tggttcgtca tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc accgggcgca
     5401 acatcatgaa aaacgccggg atgaaaaaat actccatgga actgggcggt aaatcgccgg
     5461 tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg ttcaccatct
     5521 tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag caaagcatct
     5581 acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg ggcgatccga
     5641 acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa aaagtctccg
     5701 gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc ccggataaac
     5761 cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg gtgctggcag
     5821 acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc gcctgcctgc
     5881 tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag tacggcctcg
     5941 cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt ggcattgaag
     6001 ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca tttggcggcg
     6061 taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg ttcgcggaaa
     6121 tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga gtctgatatg
     6181 ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct ctctgaactg
     6241 ccagggaaaa accacggttg ccgccaaggc gcgatcgacg ggcataaaga gatcagcaag
     6301 cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg gctggtcaac
     6361 agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag caacgagctg
     6421 ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt ggggcagctt
     6481 attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat tcccagcctg
     6541 aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa gcacttcaaa
     6601 gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg caagctgggc
     6661 gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct tgccagcggt
     6721 tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa cagctacacc
     6781 cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga aggccagttc
     6841 aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga aggcaatatg
     6901 cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg caaggtggag
     6961 tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt tttcccgctt
     7021 cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa catccgcgaa
     7081 gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc cacgggtatt
     7141 tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca gatggccgac
     7201 gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg tcgcagcctg
     7261 gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca cttcgccgcc
     7321 ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca tccgacgctg
     7381 aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg cccggtggcg
     7441 ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat aaggcgcagc
     7501 cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac accctgatcg
     7561 cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc tcgctggatt
     7621 acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt cgcctgaaaa
     7681 tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa gcaatgcagg
     7741 ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg ttcttccacg
     7801 atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg gagctggctt
     7861 tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc tacaacgcca
     7921 cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac atcgatccgg
     7981 aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc aacgccgggg
     8041 tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg atctccgccc
     8101 tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg ctgaatcatc
     8161 cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta caactggaag
     8221 ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt aagggcgaca
     8281 ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt taaggagaga
     8341 acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat cggattatgg
     8401 ctgggactga gtagcagcta cagcgcagag ttactggccg gagcaggatt cgactggttg
     8461 ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca gctacaggcg
     8521 attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc ggtgcaaatc
     8581 aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca aaacgctgac
     8641 gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg cggtgtgggc
     8701 agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca aaaagccaac
     8761 gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa cttaccgcag
     8821 attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct gagcgccgat
     8881 atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga gcaggcgatc
     8941 gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga gcaactggca
     9001 aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac caccctgctc
     9061 gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc cgtgaagccc
     9121 ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac caaaccgtag
     9181 gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc ggaccggata
     9241 aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc cattagagga
     9301 agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc gaatcagcat
     9361 aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg cctgatcgtc
     9421 tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg ctttgccgga
     9481 ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc taccaccctg
     9541 ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag tattgtcggt
     9601 gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac tgccaccatg
     9661 tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat taccgaagcc
     9721 ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta tttccgcgcc
     9781 cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg ctcgatcgtt
     9841 tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca gtggctgttt
     9901 ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg gcttgatgac
     9961 tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca agagatgatg
    10021 gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca cgccatgcaa
    10081 caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac cctggcgtat
    10141 ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat cctgcaaagt
    10201 tttagtcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc gcagatttgt
    10261 accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga acgaaggcat
    10321 cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc ggcaactgat
    10381 cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt cagcgcaatg
    10441 gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc gatcggtatt
    10501 gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat gatcggctgg
    10561 ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc gctgctggtg
    10621 attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg agcgaccccg
    10681 taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa agagtacgat
    10741 gaaagcctag gcacggacga tgtgcattat cagtcgttcg cccgcatggc ggcatttttt
    10801 ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt cctcaatagc
    10861 ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc cctgtttgtc
    10921 ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga cggtcatgtg
    10981 ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc gggcactcgg
    11041 gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa ctggcggcgc
    11101 tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct agacgggaac
    11161 acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac gcaaaactcg
    11221 acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt tttaatatgc
    11281 tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac gaactgcata
    11341 tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg ccaaaacggt
    11401 tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct gataacgccg
    11461 tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg cgctttttta
    11521 atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct gtgacgtgaa
    11581 aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc tccgcctgga
    11641 gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca aaaccgtacc
    11701 cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc ccctgcaagc
    11761 ttcaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat taatactgta
    11821 gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt caccggggaa
    11881 gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga gcgagtgaaa
    11941 gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca actgtacgac
    12001 gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac cggcagcggc
    12061 ggctataccc ataaattctt ccgcgtggcg aaaagtgccg acgacctgcg ccagcaacgc
    12121 gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac cccagactac
    12181 aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca gttcgagcag
    12241 aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa ccacgcgatt
    12301 gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt ttacatcaag
    12361 ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt tgccaccaac
    12421 tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat gggcgaaaac
    12481 ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa attaatctcc
    12541 cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta cccgctctcc
    12601 agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat cccatgggaa
    12661 aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga aggcggtttt
    12721 gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga cttcattaca
    12781 gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg tgtacaggcc
    12841 gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga ctcgatgtgt
    12901 tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc actgcaaacc
    12961 tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga acgcaacgtt
    13021 accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc gcagatcgac
    13081 cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca gcacatcaag
    13141 atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca cgaactgtat
    13201 gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg ccaggcacaa
    13261 agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc ggaatacgac
    13321 cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat gctggataag
    13381 ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc ctgcgctttc
    13441 gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag ggcgacgccg
    13501 gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca ccatcgctga
    13561 tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac ggtaagttgt
    13621 gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg ggcatgacag
    13681 gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg ctggcgcagc
    13741 cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg caggcaattg
    13801 gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca gaaggtcacg
    13861 gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa gctgcgattt
    13921 aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc tcaatcacat
    13981 atccacccga tgattaattc ttgcccaacc gctcaccgcc attcctgacc gcttacgtcc
    14041 ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg gcaacatcac
    14101 tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac ggcttcggcc
    14161 tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag tgagtgaatc
    14221 cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg cacctgtaag
    14281 ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac tattcaagca
    14341 cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag ttgcattacg
    14401 tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg tgtatctggt
    14461 ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg accccacgcg
    14521 cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca accgttacgt
    14581 gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg gtccggtgct
    14641 cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg tagtactggc
    14701 cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg gcgcatctct
    14761 cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc tgtttggctg
    14821 tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta aagccctggc
    14881 cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc tgtttatggg
    14941 tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta ttggtatcgt
    15001 gctgctggtt gcctctatct atttcggtgg catgattgct cacgatccgt actggggtcc
    15061 ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg cgtttgtttc
    15121 cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa ccttcctgaa
    15181 aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg aactgaaaat
    15241 gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg ctctgttccc
    15301 gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc tgatctcttc
    15361 cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg gctacggcgc
    15421 aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca tcatcgaacc
    15481 gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca tgcctaacct
    15541 gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag acgttaccgc
    15601 acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc agatcctgca
    15661 aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg cgccgacgct
    15721 ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca tgggcttctg
    15781 gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg atgcgggtac
    15841 tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt tcctgaaaaa
    15901 aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc tgtggggcta
    15961 cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt ggccgctgtt
    16021 cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg ttgtgctgat
    16081 taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat ggctgcttat
    16141 ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga tggaaggctt
    16201 cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc tgacggcgca
    16261 gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag gcctgagtat
    16321 tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct ggcttgcggt
    16381 gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc cggaaggcgg
    16441 cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta tccggcctac
    16501 aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat ccctagcccg
    16561 gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta ggacaggcaa
    16621 aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac gacaactacg
    16681 tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc
    16741 gtgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat
    16801 ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa
    16861 accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa
    16921 aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc cacgcagatc
    16981 aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga agacgcacta
    17041 ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct ggtcattgaa
    17101 tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca tgagatttta
    17161 tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca tgccgatgag
    17221 cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg tattctgctg
    17281 acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac
    17341 gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct tctgttcaac
    17401 accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt ccactttatc
    17461 gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc ggtagatatc
    17521 agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt
    17581 tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag
    17641 cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca tagcacgatg
    17701 gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc gggactcaaa
    17761 aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat attcctccag
    17821 aatatgaggg cattcgggct taattatctc tgaacgtgca acacttcctg cgactcctta
    17881 tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt ttcgtacatt
    17941 ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa cccactctga
    18001 ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg tgagaaaaca
    18061 atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt ttcttccgct
    18121 gcggcctgcg gtacaccaaa gaaattattt gcccggaacg ttaaggccat tgcaggtgtt
    18181 ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt atcttccagc
    18241 gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc ctgtgggatt
    18301 ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact tatattcagc
    18361 gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca cttatcaaat
    18421 ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca aacgttcagg
    18481 atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt gatgccatcc
    18541 caacgtgtta atgtatccac caactgacgg cgcggatcgc tctgtgtcaa accagatgtc
    18601 gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact ggtttggcga
    18661 ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag tcggtcgatc
    18721 tccgtaacgc gatct
//
LOCUS       DAJTMK010000076        18548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTMK010000076 DAJTMK010000000
VERSION     DAJTMK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18548
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.077"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..893)
                     /locus_tag="OO135_002206"
     CDS             complement(<1..893)
                     /locus_tag="OO135_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5398041.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSEIYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYW"
     gene            complement(1021..2973)
                     /gene="topB"
                     /locus_tag="OO135_002207"
     CDS             complement(1021..2973)
                     /gene="topB"
                     /locus_tag="OO135_002207"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5398042.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSAD
                     KKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2978..4021)
                     /gene="selD"
                     /locus_tag="OO135_002208"
     CDS             complement(2978..4021)
                     /gene="selD"
                     /locus_tag="OO135_002208"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5398043.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDTFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(4138..4689)
                     /locus_tag="OO135_002209"
     CDS             complement(4138..4689)
                     /locus_tag="OO135_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5398044.1"
                     /translation="MDALELLVNRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4850..6706
                     /gene="sppA"
                     /locus_tag="OO135_002210"
     CDS             4850..6706
                     /gene="sppA"
                     /locus_tag="OO135_002210"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5398045.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6873..7889
                     /gene="ansA"
                     /locus_tag="OO135_002211"
     CDS             6873..7889
                     /gene="ansA"
                     /locus_tag="OO135_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5398046.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFIILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7900..8541
                     /gene="pncA"
                     /locus_tag="OO135_002212"
     CDS             7900..8541
                     /gene="pncA"
                     /locus_tag="OO135_002212"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5398047.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(8702..8974)
                     /locus_tag="OO135_002213"
     CDS             complement(8702..8974)
                     /locus_tag="OO135_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5398048.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(9016..9429)
                     /gene="msrB"
                     /locus_tag="OO135_002214"
     CDS             complement(9016..9429)
                     /gene="msrB"
                     /locus_tag="OO135_002214"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5398049.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            9771..10766
                     /gene="gapA"
                     /locus_tag="OO135_002215"
     CDS             9771..10766
                     /gene="gapA"
                     /locus_tag="OO135_002215"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5398050.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVSVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10850..11734
                     /locus_tag="OO135_002216"
     CDS             10850..11734
                     /locus_tag="OO135_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5398051.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(11785..12639)
                     /gene="yeaE"
                     /locus_tag="OO135_002217"
     CDS             complement(11785..12639)
                     /gene="yeaE"
                     /locus_tag="OO135_002217"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR5398052.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(12729..13475)
                     /gene="mipA"
                     /locus_tag="OO135_002218"
     CDS             complement(12729..13475)
                     /gene="mipA"
                     /locus_tag="OO135_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5398053.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGNHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13911..15845
                     /gene="yeaG"
                     /locus_tag="OO135_002219"
                     /pseudo
     CDS             13911..15845
                     /gene="yeaG"
                     /locus_tag="OO135_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            15958..17241
                     /locus_tag="OO135_002220"
     CDS             15958..17241
                     /locus_tag="OO135_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR5398054.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            17388..>18548
                     /locus_tag="OO135_002221"
     CDS             17388..>18548
                     /locus_tag="OO135_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE4 domain-containing protein"
                     /protein_id="HCR5398055.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDLGQIYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSI"
BASE COUNT         4654 a         4698 c         4415 g         4781 t
ORIGIN      
        1 tgccagtaaa gaaatcccat tagactattt ttttgataat cttcttcgct ttcgaacaac
       61 tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc ttcaattgtc
      121 ccgttttgaa aagctgtgct tgatatcgag atcatccatg ataattccgc cgcccatatt
      181 agcttcgccg aggatttacc ggagcgatta ttagcgcaat cagagatata gtctgaggga
      241 aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc catgtttgct
      301 ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaaggc acaataaatt
      361 tcgctatctt cgatacccca ttgattctct aaagactcgc gtcttttact tatgatatcg
      421 atcgagtcaa aaggaagcac atgatattgg aaggtatctt tgccaggttc aggctttcgc
      481 ggccagaact ccagcgtttc agaccattgc ttatgataga atcgataagg tgcgatcaat
      541 tgtagcgcct gtaacttctc gatactgagc ggctcaatac ctttagcctg ataataatgc
      601 agttgttctt tttttgcttt aaaaccggcc cgaacaataa gccccatcat aattaataga
      661 taaagaaaag agcatcccgc ggtaatcagg cctctttcat tcaaaccgtt ggatgttatc
      721 gctgcgaaca caaacattac agcgacaaca catgttaaat aaaaacccca cttacaaagc
      781 agcatggcct tattttcttt aatcatccgt tcaaaattac tattaaatat ttcccagcca
      841 ttaaaagaat acttctcgct cccaggatgg ttttgtaata aaactttttt catcacattc
      901 cctgttatta catactttag aggcgataat tttatccatg caaaaaaaat atccaacgaa
      961 ttttcttgat tcgctggata tttcagaata tgactcgaat agcacgaaag attcactcgc
     1021 ttacgctatc gccccgcttc cgacttcatc tgctggcgga ctttttttcg cactacgttt
     1081 acgcggtgca gcctttttct tatcagcact gcccggagcc acaatgccgc gaaactgccg
     1141 caccggcgta cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat
     1201 aaagtcctga tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc
     1261 ggtcatgtcc ggtcgcgtcg ccatttctgg cagcgaatgg aataacgctt ttccggcgtc
     1321 ggtagagtgg atatagcgcc cttttttggt cagaaaacca cgcttgaaca gcagttcaat
     1381 aatcccggca cgggttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag
     1441 atctttgtcc tgcacaaagc gcgcgatccc ggtcatcgcc gaaagcagtg ttgcatcggt
     1501 aaaatggcgc ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc
     1561 atcgcctttc gccaccacag gcaatggcgt accgtcgttt tcttcatcgc gctctttgct
     1621 gcctaacagc gtgcgccagc ctgcttcagc aagaaaacgc gctttagcga caaatttgcc
     1681 tttggcaatg tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat
     1741 caagtactga cgggcaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc
     1801 agaactccgt gcggtcggga tgatggcgtg gtgtgcatcg acctttttgt catcccaaca
     1861 gcggttgcgt atatctggat ctaccactgg ttgcggcaac agatctggtg catgaacgct
     1921 gatggcattc atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc
     1981 ggaacgcgga taagtgatta gcttgtgcgt ttcgtacagc ttctggcaga tatcaagcac
     2041 gttctgcgcg ctcagaccaa agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa
     2101 aggcagcggc gcggattctg attcccgttt atcgttatag ctggtgacaa ttgctggttg
     2161 accactaatg cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc
     2221 atcctggtac ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc
     2281 aggtgtcacg atatgtgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc
     2341 ttcatcgcgg cgcaccacca gcccgagcac gggtgtctgc acgcgtccca cggaaagtac
     2401 gccctgataa ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta
     2461 cagccagtcg gcacgcgctc gcgccagcgc agaaacgcac aatggcacaa actcactgtt
     2521 ggaccgaaga cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca
     2581 acgctgcacc tgctggcgct tttccggtgc cagttgcaga tagtccagca cttcatccac
     2641 cagcagttgc ccttcacgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag
     2701 gaagcgtttg atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt
     2761 ttccgggaca atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc
     2821 tggctgcgcc tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca
     2881 ctcgataaag ccatcgcctt tccggtgcgg tttgggcagg acatcagcaa tggcgcgcgc
     2941 cagactcggt ttttcggcaa taaacaaccg cattgaatta acgaatctca accatggcac
     3001 gaccgccgcg cgctggcacc agttcgccaa ttgccgtcag ttcaatgcca aactcggcgg
     3061 ctgtagcttt gacctcattt tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag
     3121 tttgcggatc gcacagcaaa tcgcgcactt cacgcggcat ttcacccatc agatgaccgt
     3181 agctggcaaa gttacgttcg gtgccgccag gcactgcgcc caacttaatg tactcttcaa
     3241 cccccggcaa ttttgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac
     3301 acatttcgct caaatggccc agcagaccaa agcccgtaac gtcggtcatc gcttttacgc
     3361 cttcgatgtt ggcaaaggac gcgcctgcga tgttcatccg gcacatcact tccgtcgcta
     3421 gtccctgatg ttctggtttc agcagtgatt ttttctcagc cgtggtaaga acgccgatcc
     3481 ccagcggttt cgtcaggaac agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc
     3541 gctcggtcgg tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg
     3601 agtgaccgcc agccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta
     3661 cttcgcgggc aatttccggg gaaagtttgt taatcggcca gccgaggatc gcaatcgcca
     3721 taatcggttt gccccccatc gcgaaggtat cgctgatggc gttagtcgcc gcaatgcggc
     3781 caaaatcgaa aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg
     3841 tgccattgcc cagatcgtac accgccgcat cgtcgcgggt ctcattaccc acaagcaaat
     3901 tcggatcaac aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa
     3961 ttttacagcc gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca
     4021 tggacatctc ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt
     4081 gagagtgtct gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca
     4141 gaaataagtt acaaacggcg tcgggtccgg gacgttaatc gacgtagatg ctttcaactg
     4201 cggcgtgccg aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc
     4261 acgcactacc ggactttcag ttaatgcgcc actgcgccag atgccgccaa atccctgggc
     4321 aacggctgcc atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg
     4381 cgggacttta tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa
     4441 cggcgcatta cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg
     4501 ttccagtacg gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg
     4561 ttgcatggac ttatggtccg gcgcacgcat acccgcacgc aggatgtttt gcagttgttc
     4621 acccgttggc gcgggttcag ccaggcggga ggcgctacgg cgattgacca atagttcgag
     4681 tgcatccatt tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga
     4741 ttttgttaca gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa
     4801 ccacatctat tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct
     4861 ttggcgattt attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga
     4921 aatggtactt aacctgttct ttattttcct cgtactggtt ggtgtgggga tctggatgca
     4981 ggtcagtggt ggtgattcga aagaaacggc cagtcgtggc gcactgctgc tggacatttc
     5041 cggtgtgatc gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccagct
     5101 gcttggtgcc agttccgatc gtctgcagga aaactcactg tttgatatcg tcaacaccat
     5161 tcgccaggcg aaggacgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc
     5221 aggcggcgac cagccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag
     5281 cgggaaaccg gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag
     5341 tttcgccaat aaaatttggc tgtcaccgca aggcgtggtt gatctgcacg gttttgctac
     5401 caacggtctg tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg
     5461 cgtgggtacg tataaatctg ccgttgaacc gtttattcgt gatgatatgt caccggcagc
     5521 ccgcgaagct gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc
     5581 cgctaaccgg cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg
     5641 tttaaccaaa accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc
     5701 actggcatcg agtgccgaaa tcgaaaaagc tctgaccaaa gagttcggct ggagtaagac
     5761 tgataaaaat tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac
     5821 cggtgacagc atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca
     5881 ggggaatgtt ggcggtgata ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa
     5941 agtgaaagcg attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt
     6001 gattcgcgct gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg
     6061 cggcatggcg gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa
     6121 ccccagcacc ctgaccggtt ctatcggtat cttcggcgtg atcaccaccg tagaaaatag
     6181 tctggattcg attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc
     6241 tatcaccagg gcactgccgc cggaagcgca gcagatgatg caattaagca ttgagaatgg
     6301 ctataaacgc tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcaaattga
     6361 taaaatcgcc cagggccacg tctggaccgg tcaggatgca aaagctaacg ggctggtcga
     6421 tagtctcggg gatttcgatg atgcggttgc caaagcagca gagctcgcaa aagtgaaaca
     6481 gtggcatctg gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat
     6541 gtctggttct gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct
     6601 tgcctcggta gcctctaccg ttaaaagtga aagcgacaag ctggccgcgt ttaacgaccc
     6661 acaaaaccgt tatgcgtttt gcctgacctg cgccaacgtg cgttaagtct tgtactgagt
     6721 ggccgacaga tcgtcggcca cattattttt tacctcgacg aatcctcttc ccgctgtctc
     6781 gccccatatt tccttatccg catagtatca ggtgcgctcc tcctgcctca cgtatatact
     6841 tttgctcttt cgatatcatt catatcaata tcatgcaaaa gaaatcaatt tacgtcgcct
     6901 acacgggcgg gaccatcggg atgcagcgtt ccgagcaggg ttatattccg gtgtcaggtc
     6961 atctacaacg ccaactggcg ctgatgccgg aattccatcg cccggagatg ccagatttca
     7021 ccattcatga atatacgccg ttgatggatt cttctgatat gacgccggat gactggcagc
     7081 atattgctga agatattaaa gcgcactatg acgactatga tggttttatc attctacacg
     7141 gtaccgacac gatggcgtat accgcctctg cgctgtcgtt catgctcgag aatctcggta
     7201 aaccggtcat tgtgacaggg tcacaaatcc cgctggctga gttacgctct gacggacaaa
     7261 ttaatctgct gaatgcgttg tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct
     7321 ttttcaataa ccgattgtat cgcggcaacc gcactaccaa agcccatgcc gatggttttg
     7381 atgcgtttgc ctctccaaac cttcctccgt tactggaagc tggtatccat attcgtcgtt
     7441 tgaatacgcc acccgccccg cacggtgaag gggaattaat cgttcatcca atcacgccac
     7501 aaccaattgg cgtagtgacg atttatccgg ggatttctgc tgatgtcgtg cgcaattttc
     7561 tgcgccaacc ggtgaaagca ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa
     7621 acaaagcctt cctgcaggaa ttacaagaag ccagcgatcg cggtattgtg gtggtcaacc
     7681 tgacacaatg tatgtccggt aaagtgaata tgggtggtta tgccaccggt aacgccctcg
     7741 cccatgccgg cgttattggc ggtgcagata tgactgtaga agccacgcta accaaactgc
     7801 attacctgct gagccaggaa ctggatactg aaaccattcg caaggccatg agccaaaacc
     7861 tgcgcggcga actgacgccg gatgattaag gagactgcaa tgccccatcg cgccctgtta
     7921 ctggtcgatt tacaaaatga tttctgtgct ggtggcgcgc tcgccgtacc ggaaggtgac
     7981 agtacggtgg aggttgctaa ccgccttatt gactggtgcc agtcgcgcgg agaagcggtt
     8041 atcgccagcc aggactggca tccggcgaat cacggcagtt ttgccagtca gcacggtgta
     8101 gagccttata cgccaggcca actcgacggt ttgccacaaa ccttctggcc agatcactgt
     8161 gtgcagaaca gtgaaggcgc acaattacat ccgttactga accaaaaagc gatcgcagcg
     8221 gtgttccata aaggcgaaaa tcctttagtt gacagttaca gtgccttttt tgataacggc
     8281 cgtcggcaga aaacctctct cgatgactgg ttacgcgatc atgaaatcga tgaattgatc
     8341 gttatgggcc tggctactga ctattgcgtg aagtttaccg tgctggacgc gttacagtta
     8401 ggttataagg tgaacgtgat taccgatggt tgtcgtggcg tgaatatcca gccccaggac
     8461 agtgcacacg cgtttatgga gatgtcagca gctggggcaa cgctatatac gctggcagac
     8521 tgggaagaga cacaggggta atattagttg aattcatctc tggcctacat ttcctcaatg
     8581 acattgtagg ccagataata cttgtcagtg ttgcatttaa cctacttgca gctaaaacac
     8641 gacgtccgaa caagtatatt tgccctccct taataacagg cttaaatact agataactca
     8701 ttcacttaaa catagcaata ggctttgctg agataccaaa atcttctttt aactgctgtt
     8761 tgctcttcat gaccatctga ccatttgtgt caattgtcat atgctgtgct tcggtattat
     8821 ggcgggcttg ccacagcatc accagttgca ggcaattctc tttttgttcc tcggtcaacg
     8881 caacgccatc aggccatttc cccagttcaa cggcggtcga caaacgctgg tatacctcag
     8941 gcgtcatgct gttgataatg tcatcaagat tcataatttt tccgctcctg tggaataagt
     9001 tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt tcgccatcag tgaagcgtaa
     9061 agaagcagag ttaacacaat aacgttcgcc tgttggttgc ggcccgtcgg gaaagacatg
     9121 ccccagatgg gcatcacagt taccgcaacg aatttctatg cgctgcattc catgtgacaa
     9181 gtctttgata taacgaatgg attcttcact caccggttcg tagaaactgg gccagccaca
     9241 gccggaatca tacttggttt cggaatgaaa cagcggggca tcgcagatca aacagtgata
     9301 tacgccgtca cgcttgttat gcagtaaacg ccccgtaaat ggcggctctg tcccatgatt
     9361 ctgcgtcacg taaaactgca tctcggacaa attttttttc agttcttctg ccgaaggttt
     9421 attagccatt tgctcacatc tcactttaat cgtgctcaca ttacgtgact gattctaaca
     9481 aaacattaac accaactggc aaaattttgt cctaaacttg atctcgacga aatggctgca
     9541 cctaaatcgt gatgaaaatc acatttttat cgtaattgcc ctttaaaatt cggggcgccg
     9601 accccatgtg gtttcaagcc caaaggaaga gtgaggcgag tcagtcgcgt aatgcttagg
     9661 cacaggattg atttgtcgca atgattgaca cgattccgct tgacgctgcg taaggttttt
     9721 gtaattttac aggcaacctt ttattcacta acaaatagct ggtggaatat atgactatca
     9781 aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct gctcagaaac
     9841 gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac atggcataca
     9901 tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg aaagacggtc
     9961 atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg gctaacctga
    10021 aatgggacga agttagtgtt gacgttgtcg ctgaagcaac tggtctgttc ctgactgacg
    10081 aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact ggtccgtcta
    10141 aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct ggccaggaca
    10201 tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa gttatcaacg
    10261 ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc gctactcaga
    10321 aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct tcccagaaca
    10381 tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca gaactgaatg
    10441 gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta gttgacctga
    10501 ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt aaagccgctg
    10561 ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta tctaccgatt
    10621 tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct ctgaacgaca
    10681 acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac aaagttctgg
    10741 acctgatcgc tcacatctcc aaataagttg agatgaaact gtgatctaaa aagagcgact
    10801 tcggtcgctc ttttttttac ctgataaaat gaagttaaag gactgcgtca tgattaagaa
    10861 aatttttgcc cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc gtaaactgga
    10921 tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg cattacaggg
    10981 cgcacacctt ctctcgtgga aacctgcggg tgaagaagaa gttctgtggt tgagcaacaa
    11041 cacaccgttc aaaaatggcg tcgctattcg cggtggcgta ccggtttgct ggccgtggtt
    11101 tggtccggcg gcacaacaag gtctgcctgc gcacggtttt gcccgcaacc tgccgtggac
    11161 gctgaaatca catcatgaag atgctgatgg cgtagcgctg acttttgaat tgacgcaaag
    11221 cgaagagacg aaaaaattct gtccgcacga ctttacgctg ttagcgcatt tccgcgtggg
    11281 taaaacctgt gaaatcgatc ttgaatcaca tggcgaattt gaaaccacct ctgccctgca
    11341 tacctacttt aacgtgggtg atatcgctaa ggtaagcgtc agtgggctgg gcgatcgctt
    11401 cattgataaa gtgaatgacg cgaaagaaga tgtactgacc gacggtattc agaccttccc
    11461 tgaccgtacc gatcgcgtgt atctgaatcc gcaagattgc agcgtgatta atgatgaagc
    11521 gctgaatcgt attatcgccg taggccacca gcatcatctg aacgttgtag gctggaaccc
    11581 gggaccggcg ctttcaatta gcatgggcga tatgccggat gatggctaca aaacatttgt
    11641 ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtc accaaagaga aacctgcaca
    11701 tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg ttaatgttgt gtgccgggtg
    11761 cgatgcatcc ggcacgtatc aacatcacac catatccagc gcagtttttc cttttggtgc
    11821 cggatacgct ttatccagca tggttaattc cgctgacgaa agttcgacct caagcacagc
    11881 cgcattctgt tgaacatggg caatcgtggc cgcttttgga atcgccatca caccctgatg
    11941 gctgattacc ctcgccaaca atacttgtgc cgagctgata ttgtgagcat gtgcaatttc
    12001 gttgactacc gcgtttttta acagtccatt gcgcaaccgc ccggcctggg ctaatggact
    12061 gtaagccatc accggcatct gctgttgctg gcaccagggg agtagatcgt actcaatccc
    12121 tcgtgaaccg agatggtaaa gcacctgatt agtggcacac tgatttcccc ccggcagctg
    12181 ccagagttcc tgcatatcag cataatcaag gttagaaacg ccccagcggc ggatttttcc
    12241 ctgggcgatc aatttttcca tcgctgcaac agtctcttca aaagcgaaac tgccagacca
    12301 gtgtaataag tagagatcga gataatcagt attgagacgg cgtaaactgg cttcgcatgc
    12361 atttatcgct ttttgcctgc cagcattcca cggatagact ttagagacga gaaagacgtt
    12421 atcacgcaga ccggttaatg cttccccaac caccttttcg gcaccgccat cggcatacat
    12481 ttcggcggta tcaatgaggg ttaaaccgag ctcaatgccc gcgcgtagtg cagcaacttc
    12541 tgttttgcgc tgactggcat cttcgcccat ataccatgtt ccctgcccta tggctggcag
    12601 tgagacatcg ccactaaatt gaatcatttt ttgttgcatt gtttcctccc aggtattgca
    12661 ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag
    12721 atgcattatc agaatttgta ggtgatcccg gtagaaatca ggccagtcca ggatttatcc
    12781 accatcgggc tgtcagtaac ttcatcagac agacgggtat agcgcgcggt accgtaaaca
    12841 ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca ggtaagggct ccagctgtcg
    12901 ttcgggttat agccacgcag accgctgcga gcggactctt tgcgcgatac gccataatag
    12961 tattcgttct ggttttcgct gttccactgc acaccaatac ccggagtcac ggtcaggcca
    13021 ccgttggtgt aacgatacaa ccaggccata tcccagacaa tgccgttgct gttatccagg
    13081 gtatcgccag ccagggtggt acgcaggtaa ccgtactggg taaagtgagc ataagacaga
    13141 ccagccatca tggtgctctt acggtcatcc aggtgacgca tttggtgatt gccactgtct
    13201 ttcgctttga agtaaagcgg cgaccagtaa gcggtaattg aaagtttatc cgttgcgtca
    13261 ttccacaggt agtaaccacc acctaagcca cggaaccaga agttatcgcc ttcatagttg
    13321 attaccggta ctgggtaaac atcggtatcg taatctttat atgggtgctc aacgacacct
    13381 acgcctgcgc ccaaggaaaa tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata
    13441 agcactccaa gtgccagaag tttgagtttg gtcacaatta atcattcctt aaacaaatgt
    13501 ttagcgggcg acaaagttta cccgtcaata catcgaaacc caaccttttt acgttttcat
    13561 tttttaaagt aactgtttaa ttttcctgcc gcggatgaca ccgcgcttac agccaaatga
    13621 atttagcgtt actggcgagc ctggtcttta cattaattat gcaaaattta tggatgagtt
    13681 gttgatatgc cattgaaatt aagaaagccg tgcaggcaag ttttccattt gccatctacg
    13741 cttaattttg aaggtgtatc accgggcacg ttgttctcat cgtcgataaa atggcatgag
    13801 agttgctgtg ttttagcaag agacgtcgtt cagtttacct cttccgggag cctctactat
    13861 tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc atgaatatat
    13921 tcgatcacta tcgccagcga tatgaagctg ccaaggacga agagttcaca ctgcaggagt
    13981 ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag cggctattga
    14041 tggctatcgg tgagcctgtc atggtcgata cagcccagga acccagactt tctcgactct
    14101 tttctaaccg ggtcattgca cgttatccgg cgtttgaaga gttttacggc atggaagacg
    14161 cgattgaaca gattgtctct tatctgaaac acgcggctca ggggctggaa gagaagaaac
    14221 aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct gagcgactga
    14281 aatcattaat gcagctcgta ccgatttatg tattgagcgc caacggtgag cgtagcccgg
    14341 tcaacgatca tccgttctgt cttttcaatc cgcaggaaga tgcgcagatt ctggaaaaag
    14401 agtatggcat tcctcgccgt tatctcggca ccatcatgtc gccgtgggcg gcaaaacgcc
    14461 tgcatgaatt tggtggcgat atcactaagt tccgggtagt gaaggtctgg ccgtcaattc
    14521 tgcaacaaat tgctatcgcc aaaacggaac ccggcgatga gaacaaccag gacatctccg
    14581 cgctggttgg gaaaatcgat attcgtaaac tcgaacacta cgcgcagaat gacccggacg
    14641 cctacgacta ttccggtgcg ctgtgccgcg ccaatcaggg gatcatggaa ttcgttgaga
    14701 tgtttaaagc accgataaaa gtgctgcatc ccttgttaac cgccactcag gaaggtaact
    14761 acaacgggac ggaaggtatc tccgccctgc cgttcaacgg gattattctc gcccactcga
    14821 acgagtccga atgggtcact ttccgtaata acaaaaacaa cgaagccttc ctcgaccgtg
    14881 tttacatcgt gaaggtgccg tattgcttgc gcatttccga agagatcaaa atctacgaga
    14941 aattgcttaa tcacagtgaa ttgactcacg ccccatgcgc ccctggcacg ctggaaacac
    15001 tgtcacgttt ttccattctt tcgcgcctga aagagccaga aaactccagc atttattcaa
    15061 agatgcgggt ttatgatggc gaaagtctga aagacaccga tcccaaagcc aagtcgtatc
    15121 aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg
    15181 cgtttaagat cctctcccgc gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg
    15241 tccatctgtt ctacgtcctg gaacagcaga tcgagcgcga gcagttcccg caagagcagg
    15301 cagaacgcta tctggagttc ctgaaaggtt atctgatccc gaaatatgcc gagtttatcg
    15361 gcaaagagat ccagacggcc tacctttaat cctattccga atatgggcaa aacattttcg
    15421 accgttatgt tacctacgcg gatttctgga ttcaggatca ggagtatcgc gatccggata
    15481 ccgggcagct gtttgaccgc gagtctctta acgccgagct ggagaaaatc gagaaaccgg
    15541 cggggatcag taatccaaaa gatttccgca acgagattgt taacttcgta ctgcgcgcca
    15601 gagcgaataa cagcggacgc aatccgaact ggaccagcta tgaaaaactg cgcacggtta
    15661 tcgagaagaa aatgttctcc aataccgagg agctgttgcc ggttatctcg tttaacgcca
    15721 aaacgtcaac cgacgagcag aagaaacacg acgactttgt cgaccgtatg atggaaaaag
    15781 gctacacccg taaacaggtg cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt
    15841 cttaacaacc ctggcccggt acacatgcta ccgggcctac aacgacagcg aaccgtgggc
    15901 cagagaaatg gcaacacagg cgtagcatac agttggcaaa tgtagtacgg ggggcatatg
    15961 acctggttta ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt
    16021 tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat gaataagcgt
    16081 tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga tattagcgaa
    16141 ccgatgtttc atcaggggcg tggcggcctg cgccaccgcg tacatccggg caatgaccat
    16201 ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg gcggaggttc cggcagtggt
    16261 cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca gatttcgaaa
    16321 gatgagtatc ttgatctgct ctttgaagat ttggccttac cgaatctgaa acaaaaccaa
    16381 caacgccagc tgaccgaata taaaacgcat cgggcgggtt ataccgctaa cggcgttcca
    16441 gccaatatca gcgttgtgcg ttccttgcag aactcactgg cgcgacgcac agccatgacg
    16501 gcgggcaagc gacgggaact tcatgcgctg gaagagaatt tggccatcat cagcaacagt
    16561 gaacctgcgc aactgctgga agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc
    16621 aaaattgaac gcgtcccttt tattgacacc ttcgatttac gttacaagaa ctacgagaag
    16681 cggcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc cggttcaatg
    16741 gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct gttcctcagc
    16801 agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaagc gaaagaagtc
    16861 gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccattgtttc cagcgccctg
    16921 aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa tatttacgct
    16981 gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca tgaaatcctg
    17041 gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac ccgtcgtgca
    17101 catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg
    17161 cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt tcataaacaa
    17221 aatgcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc tgatttttag
    17281 cttactgtaa tttatctcat ttattacata cattagctta caatcgcttt aaatatgaca
    17341 gcataacctt tacataattt agttccagaa aacaatcatt cggaaaaatg attcagtcaa
    17401 cacgtatttc catggggtta ttctttaaat attttttatc gttaacgaaa attgatcttg
    17461 gtcaaatcta tatatctctg ccatcaataa aatccagcac tcacattgct ctccttttta
    17521 tggtttctat gggtacacaa aaattaaaag ctcaaagctt ttttattttc agtttattgc
    17581 tgacattaat tttattttgc attactacct tatataacga aaacacaaat gtaaaactca
    17641 tcccacagat gaattacctg atggttgttg tggctttgtt tttccttaac gccgtcattt
    17701 ttcttttcat gttaatgaaa tatttcacta acaaacaaat tttaccaaca ctcattttaa
    17761 gccttgcatt cttaagtggc cttatctatt tagttgaaac cattgtaatt atccataaac
    17821 caattaacgg cagtacactg atccagacaa agtcgaatga tgtttctatt ttctatattt
    17881 tccgccaact cagttttatt tgtttaacct cgctggcact cttttgttat ggaaaagaca
    17941 acatccttga caacaataag aaaaaaacgg gaatcctgtt gctggcgttg attccttttt
    18001 tagtttttcc ccttctggca cacaatctga gcagttataa cgctgactat tctttgtatg
    18061 tcgtcgacta ctgtccggac aaccatactg cgaccagggg aatcaactat acaaaaatat
    18121 tggtttgtct gtgggcattt ttactgttct ttattatcat gcgtacacga ttagccagcg
    18181 aactatggcc gttaatagca ttattatgtc tggcatcgct atgctgcaac ttacttctac
    18241 tgactctgga tgagtataat tacaccatct ggtatatcag tcgcgggatt gaagtttcca
    18301 gtaaactgtt tgttgtgtct tttctgattt ataacatttt tcaggagctg caactctcca
    18361 gcaaactggc agttcatgat gtgctgacca atatttataa tcggcgctac tttttcaaca
    18421 gcgtagagtc attattgtcg cgacctgttg ttaaggactt ctgtgtcatg ctggttgata
    18481 ttaatcagtt caaacgcatc aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg
    18541 tttcaatt
//
LOCUS       DAJTMK010000077        18518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTMK010000077 DAJTMK010000000
VERSION     DAJTMK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18518
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.078"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..149
                     /locus_tag="OO135_002222"
     CDS             <1..149
                     /locus_tag="OO135_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398056.1"
                     /translation="FDIGVLLSNREFTEDAIKMRKYEPYLLNDNSILSRIALLELGII
                     GEQQ"
     gene            146..334
                     /locus_tag="OO135_002223"
     CDS             146..334
                     /locus_tag="OO135_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR5398057.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            430..2904
                     /locus_tag="OO135_002224"
     CDS             430..2904
                     /locus_tag="OO135_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5398058.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQNTENSAGKINPVTAAICREYKQTWKTLDEELAYALWPGDIDTGNIDGSIHRWA
                     KNEVIDKDREDWKRISASMRKQPDAVRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDDEGTNQGTAGTLPSPVPETDAVETAMPDNEKTECEVEDEPSVEREGPFY
                     FLFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTIA
                     QPEPVKVTADEVNKIMQAANISQPDANNLLAVSRGEFVAGISDPNDPKWVKGIETRDS
                     VNQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDC
                     GAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTDGNQHHDSDNETGETADH
                     SIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTI
                     DLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVW
                     GNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHN
                     ALDDARYQAKYVSVIWQKLIPNQADF"
     gene            2969..3217
                     /locus_tag="OO135_002225"
     CDS             2969..3217
                     /locus_tag="OO135_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5398059.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            3195..4325
                     /locus_tag="OO135_002226"
     CDS             3195..4325
                     /locus_tag="OO135_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5398060.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMRSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDVITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(4371..5009)
                     /gene="ompW"
                     /locus_tag="OO135_002227"
     CDS             complement(4371..5009)
                     /gene="ompW"
                     /locus_tag="OO135_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5398061.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            5249..5338
                     /locus_tag="OO135_002228"
                     /pseudo
     CDS             5249..5338
                     /locus_tag="OO135_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5366..6109
                     /locus_tag="OO135_002229"
     CDS             5366..6109
                     /locus_tag="OO135_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5398062.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            6139..6678
                     /locus_tag="OO135_002230"
     CDS             6139..6678
                     /locus_tag="OO135_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5398063.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6783..7181
                     /gene="yciA"
                     /locus_tag="OO135_002231"
     CDS             6783..7181
                     /gene="yciA"
                     /locus_tag="OO135_002231"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5398064.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(7221..7949)
                     /gene="tonB"
                     /locus_tag="OO135_002232"
     CDS             complement(7221..7949)
                     /gene="tonB"
                     /locus_tag="OO135_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5398065.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            8164..8460
                     /locus_tag="OO135_002233"
     CDS             8164..8460
                     /locus_tag="OO135_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5398066.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            8760..10013
                     /gene="kch"
                     /locus_tag="OO135_002234"
     CDS             8760..10013
                     /gene="kch"
                     /locus_tag="OO135_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5398067.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10068..10241)
                     /locus_tag="OO135_002235"
     CDS             complement(10068..10241)
                     /locus_tag="OO135_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5398068.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            10384..11844
                     /gene="cls"
                     /locus_tag="OO135_002236"
     CDS             10384..11844
                     /gene="cls"
                     /locus_tag="OO135_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5398069.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            11879..12208
                     /locus_tag="OO135_002237"
     CDS             11879..12208
                     /locus_tag="OO135_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5398070.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(12261..13265)
                     /gene="oppF"
                     /locus_tag="OO135_002238"
     CDS             complement(12261..13265)
                     /gene="oppF"
                     /locus_tag="OO135_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5398071.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(13262..14275)
                     /gene="oppD"
                     /locus_tag="OO135_002239"
     CDS             complement(13262..14275)
                     /gene="oppD"
                     /locus_tag="OO135_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5398072.1"
                     /translation="MSVIETATVPLAQQQAGALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGHLCACFKPVE
                     ELL"
     gene            complement(14287..15195)
                     /gene="oppC"
                     /locus_tag="OO135_002240"
     CDS             complement(14287..15195)
                     /gene="oppC"
                     /locus_tag="OO135_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5398073.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSTPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVISHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(15210..16130)
                     /gene="oppB"
                     /locus_tag="OO135_002241"
     CDS             complement(15210..16130)
                     /gene="oppB"
                     /locus_tag="OO135_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5398074.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPIRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(16216..17847)
                     /gene="oppA"
                     /locus_tag="OO135_002242"
     CDS             complement(16216..17847)
                     /gene="oppA"
                     /locus_tag="OO135_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5398075.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            18131..18427
                     /locus_tag="OO135_002243"
     CDS             18131..18427
                     /locus_tag="OO135_002243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398076.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
BASE COUNT         4905 a         4470 c         4385 g         4758 t
ORIGIN      
        1 gttttgatat tggtgttctg ctcagtaacc gggagtttac ggaagatgcc attaagatga
       61 gaaaatatga accttatctt ctcaatgata attccatact ttccagaatt gcccttcttg
      121 aacttggtat tatcggagaa cagcagtgac ttcagcattt gcactggtga tgaccgtttt
      181 tcttataacg ggtgagccac aaaatgtgat taccggaatt tatgacagta agtcatcctg
      241 cattcaggta agggacgaac aaaaaatccc cggtgaatgc ctcccgttaa aaaaagtatc
      301 gctgaacctg aataacgaaa taccggctgg ataacccgcc agccatatta acgccatacc
      361 aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct gtaatgaggt
      421 taaaacaaaa tgagtaaagt ctttatttgc gccgccattc cggacgaaca ggcaataaag
      481 gaagaaggtg ccgtcgctgt agccactgcc attgaagccg gtgatgaacg tcgcgcccgc
      541 gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg cgcttataaa
      601 tttcttgtct gcgaggataa acccggtata ccccgccctg ccctcgattc ctgggatgct
      661 gaatatatgc aggaaaaccg ctgggatgag gaatccgctt cctttattcc ggtcgaacca
      721 gaatccgatc cgatgaacgt caattttgac aagctgtccc ttgaagtaca gaacgcggtc
      781 ctggttaagt tcggtacatg tgaaaacatc accgttgata tggcgattga cgcgcaggaa
      841 ttactgcagg aagacgttgc tacctttgac gggcatatcg ttgaagcact gatgaaaacg
      901 cctgaaatta acgctatgta tccggaacgc aaactgttcg ctatcggatg ggttaaacac
      961 aaatgtaatc cgggtgccaa atggcccgaa attcaggctg aattacgtaa ctggaaaaaa
     1021 cggcaggacg cagagcgcaa agagactgga aaatacacgt ctgttgttga tctcgccagc
     1081 gccagagtca atcaacagaa cactgaaaac tcagcaggaa aaatcaaccc agtcactgcc
     1141 gccatttgtc gcgaatacaa gcagacatgg aaaacgctgg atgaagaact ggcctacgct
     1201 ctctggcctg gcgatattga taccggaaac attgacggca gcatccatcg ctgggcaaaa
     1261 aatgaagtta tcgacaaaga tcgcgaagac tggaagcgca tttccgcatc aatgcgcaaa
     1321 caacccgatg ccgttcgcta cgaccgtcag actatttttg gccttgtccg tgagcgtccg
     1381 atcgacattc acaaagatcc cgtagcactg aacaaataca tcactgaata cctgactacc
     1441 aagggcgtgt ttgaagatga cgaaggaaca aatcagggca cagctggtac tctcccgtca
     1501 ccagtaccag aaactgatgc agtggaaacg gcaatgccgg acaacgaaaa aaccgaatgc
     1561 gaagtggaag acgaaccatc tgtagagcgt gagggaccgt tctacttcct tttcaccgat
     1621 aaggacggcg aaaaatacgg tcgcgcaaac aaactttctg gtctggaaaa agcactggcc
     1681 ctgggagcta cggaaatcac aaaagaggaa tacttcgcac gtaaaaacgg cacgtactca
     1741 ggttcacaac aaaatactgg tgcatctgac acgatcgcac aaccagagcc ggtaaaagtt
     1801 accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccaat
     1861 aatttgcttg ctgtatcacg tggtgaattt gttgcaggga ttagcgaccc gaatgatccg
     1921 aaatgggtga aggggattga aacccgcgat tcagtgaatc agaaccagca agaaacggaa
     1981 cagaacggcc agaaagcgga acaaaacagc ccaaatgcgt tacaaaacga gccagaaacg
     2041 aaacagcctg aaccagtggc gcaacaggaa gtggaaaaag tctgcaccgc ctgcggtcag
     2101 accggcggcg gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac ataccaggaa
     2161 acattcgatg aagagtatca ggttgaagtt caggaagatg atccggagga aatggaaggc
     2221 gctgaacatc cacacaagga gaacactgac ggcaatcagc atcacgatag cgataatgaa
     2281 actggcgaga cggcagatca ctcaattaag gtgaacggtc atcaagaaat cacatccacc
     2341 agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc
     2401 ccgatcatct caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa
     2461 tttagtaaga ctatcgatct ggaaactgct ggcggggtca ttgatcggga caccattaaa
     2521 tggtggctta agcaatcacg cgaagcgcaa tctgccatta tgaccgatga aatcccgtta
     2581 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
     2641 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
     2701 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
     2761 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
     2821 aatgcacttg atgacgcccg ttaccaggca aaatacgttt cagttatctg gcaaaaactg
     2881 atcccgaatc aggctgattt ttaatgttca accccggtcg ttgcccacca gctatagtgg
     2941 cggcgaccat gattagcgaa cgacgctcat ggcaagactt attctgctca ctgagtgggc
     3001 aaaagaggaa ttcagtgaac cggtcccaac tccgagtacg ttaagtaaat acgctaaagc
     3061 cggaatgata tttcctctcc ccaaaaaagt tggaagacgc tggcgagtgg atccgcaagc
     3121 tcgctttgtc ggaatggtaa acaagccgga ggtgatcgcc acagatcacc ctgctttgaa
     3181 gaggatactg gaagatggcg cgcccgcgaa aatataaaac cgatgttccg ggattatctc
     3241 cgtattttga taaaagaaat aacaaagttt actggcgtta caggcatccc ataacaggca
     3301 aaaatcacgg tctcggcagt attgaccaga aactggcaga aactattgca gcagaagcga
     3361 acagccgtct tgcccggcag caaatggaac aaatgcgcag tctgcaggag aaaattatta
     3421 gtgataccgg cggttcatca accgttacca tttttctgaa taattacaga aaaattcaac
     3481 aggaaagata tgaaaacggc gagatcaaac tcaacacgct gaaacagaaa gcggcccctc
     3541 tcagggtatt tgatgaacgt tttggcacca gaccgttaga tgtcataacc gtaaaagatg
     3601 tggtatcagt actggaagag tacaaggcca gaggacataa cagaatggga caaattttca
     3661 ggaaagtact gatcgatgtt ttccgggaag ctcagcaaac gggcgatgtc ccgccaggct
     3721 ttaaccctgc agaatcggca aaaaaaccgc aggtgcggat atcaagacag cgactgactt
     3781 ttgatgagtg gatgatgatt tataacgcag cggaaaagga tggttacttt ttacagcgcg
     3841 gtatgctgct ggcactgatg acaggccagc gcctttcaga tatttgcaaa atgcaatttt
     3901 cggatatccg ggatggttat cttcatgtcg aacagcaaaa aacaggaacc cggattgcca
     3961 tccctctggc tctgcgttgc gataaattaa atctcaccct ggatgatgtg gtgtcatcct
     4021 gccgcgattg cgttcttagt ccgtggctat tgcaccacca tcacgcgaaa gggacagcta
     4081 agcgcggcgg gatggttaag ccagcaacat taaccgttgc atttaaaaaa gcccgggatt
     4141 ctgtggatta caactggcgt gctaatggca cccctccctc tttccatgag cagagatctt
     4201 tatcagagcg attgttcaga gagcaggggg ttgataccaa aattttgcta ggccattcga
     4261 atcaaaaaat gaccgatatt tacaacgacg cacgcggtaa ggaatggaaa aaactggtca
     4321 tttgatgacc agttttgcag aggggttttg cagaggtttt gcagagaaaa ttaaaaacga
     4381 tatcctgcgg agaacataaa cacccacgga tccagtcgta ccgagtcttt tacggtagta
     4441 acaccagatt tgtacttagc cgtggtatcg atatccatgt accacactga catgttaacc
     4501 aaccagtcac ggttaatcag ataatcaacc cccacctgcc cggcagctcc ccaggaatct
     4561 ttcagactga gatcggaaag ccctgcctct ttgccatgat cgttaaatcc attatcaaag
     4621 aaggtggtgt agttaatacc tgccccaacg taaggacgga atttgctgct ggcatcacca
     4681 aaataccact gcgccatcag tgttggcggc agatggtgaa cggttgcaat atcgccggtc
     4741 gcccgggtgc cgattttatg gcggaacggc gtcgctgcca gtaattccac accaatgttg
     4801 tcggtcgcca tataagtaaa cgtcaagccc agttgagtgt tattggtcac gctgaatcca
     4861 cccagacttc ctaacgtacc accagcacct tctgttggac gtacggttgc agaacctgca
     4921 cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc cagagagaag agttgttact
     4981 gccaaagccg ccactgttaa ctttttcata tccgctccgt cgttatggtt ataaaaacgt
     5041 gacgaatata cctacattgg agtaacaaat gatcctacat agatcacatt acatatgata
     5101 attcaccctt tattgatctg gattaattta gaagttgcat tgagaatcct ggttaacgtg
     5161 atttaaatca atttttaaat attttcgcta cgcaaatatt aactcgatcg ccacttgccg
     5221 cggtttgccc acgcctgaag gccagcaatt acctccgctt tacaaagata tgcttttcca
     5281 tacaagacct gatgctgcca cccgaggctt aaccagggta caattgcccg ctaattaaca
     5341 cctgcataaa actcaaggag agtgcatgtc tatcacggcg cagtccgtat accgtgacac
     5401 cggaaatttc ttccgtaatc aatttatgac cattctgttg gtatcgttgc tatgtgcgtt
     5461 tatcacagtg gtgttagggc atgttttctc acccagtgat gcacagcttg cgcagctcaa
     5521 tgacggcgtg cccgttagcg gcagtagtgg gttgttcgac ctggttcaga atatgtcacc
     5581 ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc actttttcag gattaatcgg
     5641 taacgccatt ctcgccggag gcgtaatatt aattatccag ctggtgtctg cgggtcagag
     5701 agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata ttgccaaagt tatttattct
     5761 gatttttctg actacccttt tagtacagat tggcatcatg ctagtggtcg ttccgggaat
     5821 tatcatggcc attttactgg ccctggcacc ggtgatgctg gtacaggaca aaatgggcat
     5881 ttttgcctcg atgcgtagca gtatgcggct gacttgggcg aatatgcgtc tggtggcacc
     5941 cgcagtactg agctggttgc tggcaaaaac actgttgctg ctttttgcct cttcttttgc
     6001 cgcattaacc ccggaaattg gtgccgtact ggcgaacacc ttgagcaacc tgatttcagc
     6061 cgtattgctc atctatctgt tccgtctgta tatgttgatt cgccaataac cttaagcatt
     6121 tgattacgga atcgtaaaat gaagcagttt cttgattttt taccgctggt tgtctttttc
     6181 gcgttttaca agatttatga catctatgcg gctactgcgg cgctgatcgt cgccacggcg
     6241 attgtgctta tctatagctg ggttcgcttt cgtaaggttg agaagatggc cctgatcact
     6301 tttgttctgg tggtcgtctt cggtggcttg acgctgttct tccacaatga tgagtttatt
     6361 aaatggaagg ttacagtcat ttatgctctg tttgcgggag ccctgttagt cagccaatgg
     6421 gtgatgaaaa agccgctaat tcagcggatg ctgggtaaag aactcacgct gccgcaaccg
     6481 gtatggtcga agctgaatct ggcctgggct gttttcttta tcctttgcgg tctggcaaac
     6541 atctacatcg cattctggct gccgcaaaat atttgggtca actttaaagt ctttggactg
     6601 accgccctta ccttaatctt cacattgtta agcggtatct atatctaccg ccacatgccg
     6661 caggaagata aatcctaacc agactgccag tccttcgggg ctggcgctgt atctcctctg
     6721 ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt ttccttttaa gtcggtttta
     6781 ccatgtctac aacacataac gtccctcagg gcgatcttgt tttacgtact ttagccatgc
     6841 ccgccgatac caatgccaat ggtgacatct ttggtggttg gttaatgtca caaatggata
     6901 ttggcggcgc tattctggcg aaagaaattg cccacggtcg cgtagtgacc gtgcgggttg
     6961 aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt ggtgtgctgc tatgcacgct
     7021 gtgtccagaa agggacgaca tcggtcagca ttaatattga agtgtgggtg aaaaaagtag
     7081 cgtctgaacc aatcgggcaa cgctataaag cgacagaagc attatttaag tatgtcgcgg
     7141 ttgatcctga aggaaaacct cgcgccttac ctgttgagta atagtcaaaa gcctccggtc
     7201 ggaggctttt gactttctgc ttactgaatt tcggtggtgc cgttaatttt aaacaggata
     7261 ttcaccacaa tcccactgcc tggcttaccc ggctcataac gccatctgcg catcgcattt
     7321 ttcacctcac gctcaaacat gttcgcaggc ttggctgaga ggatttgtac gttatccacg
     7381 cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc cttcaatgcg caatgcctgt
     7441 gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc gtggtcctga agccacactg
     7501 gtaaccggct tgctggttgc agccgttgct gtacttgatg tcaggcgtgc cggtgccgta
     7561 ttttcaaacg gtgatgccgg acgcgactct acgggtttga catcgcgttt tggctgctcc
     7621 tgtacctttt tcaccggctt cggttttggc ttaggtttcg gcttcggctt ttcaatgacc
     7681 accggtgctt cttttggcgg ttcggggatc ggctcaggtt ccggttctgg ctctaccacc
     7741 ggctccggtg gcggctgaac ggcttgtggc ggttcgagat cagcaggcgc aaccatcgtg
     7801 acagaaattg gctgcgcagg cgcaggtagt tcaataacct gatgtaccga ggtatagagc
     7861 agacccgcca caacagcacc atgaatgcag accgaaagta acgtcggcca ggggaagcgg
     7921 cgaggtaaat caagggtcat tgaggtcata atcatttcag tagaaaaacc aagcctcgat
     7981 tttaaatgca aatagcaatc atattcaata aggcaagacg atcaggatca gtgaaaatgt
     8041 catttttacc gtaattaacc ttacgttatg catggtctta acaaagacat catctttccg
     8101 ttgcagatct tcgccctttc aaataacgta ctttacaact ttcccgaaca aggagttgtg
     8161 cccgtgttgt atgttattta cgctcaagac aaagctgata gcctcgaaaa acgcctttcc
     8221 gttcgtccgg cacatttagc acgtttacag ttactgcatg atgaaggtcg tttgttgaca
     8281 gctggtccaa tgccagcggt agacagtaac gatccagggg ctgcgggatt tactggctca
     8341 accgttattg ctgaatttga atcgctggaa gcagctcagg cctgggccga tgctgacccg
     8401 tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac catttaagaa agtgttctga
     8461 atagagattc aatatttaag ggcacggttt ttgcaaccgt gttcttgttt ggcctttcct
     8521 tatagccggt cagtgcattc accaccacaa gcagataata actttatctg tatttcttta
     8581 tatgatgtga gcatattcag gaaaaatgac ctttcctgag gtacagttgc tcgctcaaaa
     8641 cctttgatta ctgcggataa agtcatctac tattgtttaa cacattcacg cgctgcatgt
     8701 atgttcagaa aatgtgcacg ctaagataat taaacaaaat tgtggttaca gaggtatcag
     8761 tgagtcactg ggctacattc aaacaaacag caacaaattt atgggtgacc ctacggcacg
     8821 acattctcgc gctggccgtc tttttaaatg gattgcttat ttttaaaaca atctatggta
     8881 tgtcggtcaa tttgctagat attttccata tcaaagcatt ttcagagttg gatctctcct
     8941 tgctggcaaa cgccccacta tttatgctcg gcgtctttct tgtcctgaac tccattggct
     9001 tactgttccg ggcaaagctc gcatgggcaa tcagtatcat tttgttgttg atagcgctaa
     9061 tttataccct gcatttttat ccctggttga aatttagtat tggattttgc atttttacgc
     9121 tggtgttttt gctgatactg cgcaaagact tctcccacag tagcgccgca gccgggacaa
     9181 tttttgcatt tattagtttc acgacattac tgttttactc aacctacggt gcgctttatt
     9241 taagcgaagg ttttaatccg cgaatagaaa gtttgatgac cgcgttctat ttttcgatag
     9301 aaaccatgtc aaccgtcggc tatggcgata ttgtccctgt ttctgaatca gcacgattgt
     9361 tcactatttc ggtcattatt tccggtatta ccgtttttgc cacatctatg acctcaattt
     9421 ttggtccgct tatccgcggg ggattcaaca aacttgtaaa aggaaacaat catacaatgc
     9481 atcgtaaaga tcattttatt gtttgcggac attcgattct cgccatcaat acgattctgc
     9541 aactgaatca acgcggacaa aacgtaacgg ttatcagcaa cttgcctgaa gatgatatca
     9601 agcaacttga gcaacgctta ggcgataacg ctgatgttat ccccggtgac agtaatgaca
     9661 gttcagtatt aaagaaagcg ggaatcgatc gttgcctggc cattctggcg ctgagtgata
     9721 acgatgcaga taacgcgttt gttgtactct cggcaaaaga tatgagcagt gatgtcaaaa
     9781 cagttctcgc cgtcagtgat agcaaaaacc tgaataagat taagatggta catccggata
     9841 tcattctctc accgcaactg tttggcagcg aaattctggc gcgagtttta aatggtgaag
     9901 agattaataa tgatatgctc gtttcaatgt tgttgaactc cggtcatggc attttcagcg
     9961 ataacgatga acaagaaacg aaagctgaca gtaaagaatc ggcgcaaaaa tagagtttaa
    10021 agatggagga gtaaacttat tgggcaccgc aatggtgccc agtacattta gatattgtag
    10081 atcgcaaaca ataacgagtt acgctgtttg tttagaatgc acttcctgat gctgtggata
    10141 cgcatatttc ggcgcgattg cccctcaagc caacgcgatt tacgtcggct agcctgacgc
    10201 tgcatgcgcc agcgccccac ttccgttcta ctccgcttca tgttaactac tctatgcaat
    10261 aacagaatgg tcattatacc cccgcccccg acgctgacca gtgcttttcc cgcgttttta
    10321 tttgccagat gaatccatat gcgtaaactc ataacaatgc gctttcaaaa ggatttctaa
    10381 tctatgacaa ccgtttatac gttggtgagt tggttggcca ttctgggata ctggttgctc
    10441 attgcaggcg taactttacg cattctaatg aaacgacgcg cagttccctc cgcgatggcc
    10501 tggctgttga ttatttacat tctgccgtta gtcggaatta ttgcctatct tgccgttggc
    10561 gagctccatt taggcaaacg ccgcgctgag cgcgccagag cgatgtggcc ttccaccgca
    10621 aaatggctta acgaccttaa agcctgtaag catatcttcg ccgaagaaaa tagcagtgtc
    10681 gctgcgccat tattcaagct ttgcgagcgt cgtcagggga tcgctggggt caaagggaat
    10741 cagctacaac tgatgaccga gtcagatgat gtgatgcagg cgttaatccg cgacatccag
    10801 ctcgcgcgcc ataatattga gattgtgttt tatatctggc agcccggcgg catggcggac
    10861 caggtggctg aatcattaat ggccgctgcg cgacgcggca ttcattgccg attgatgctc
    10921 gactccgccg ggagtgtggc ttttttccgc agcccgtggc ccgagctaat gcgtaatgcc
    10981 ggtattgaag tggtcgaagc cttaaaggtc aatctgatgc gtgtgttttt acgccgtatg
    11041 gacctgcgcc aacatcgcaa gatgatcatg atcgataatt acatcgcgta caccggcagc
    11101 atgaatatgg tcgatcctcg ctacttcaaa caagatgcgg gcgtagggca atggattgat
    11161 ctgatggcgc gtatggaagg ccccatcgcc accgcgatgg ggattattta ttcctgcgac
    11221 tgggagattg aaaccggaaa acgtattctg ccgccaccac cagatgtcaa tattatgccg
    11281 tttgaacagg ccagcggtca caccattcac acaattgctt ctggccccgg ctttccggaa
    11341 gatctcattc accaggcatt attgactgcg gcttattcgg cgcgtgaata tttgatcatg
    11401 accacgccct actttgtgcc aagcgatgat ttacttcatg cgatttgcac ggcggcacag
    11461 cgcggggtgg atgtcagtat tatccttccg cgaaaaaatg actcgatgct ggtcggctgg
    11521 gccagtcgcg cattctttac ggaacttctt gctgctgggg ttaaaattta tcagtttgaa
    11581 ggcgggttac tgcataccaa gagcgtgctg gtcgatggcg aactaagtct ggttggcacc
    11641 gttaaccttg atatgcgtag tctgtggctg aatttcgaga ttaccctggc aatcgacgat
    11701 aaaggttttg gtgcagacct tgccgccgtt caggacgatt atatttcgcg ttcacgtctg
    11761 ctcgatgccc gtttatggct aaaacgtcca ttatggcaac gtgtcgccga gcgactgttt
    11821 tacttcttca gcccgttgct gtaaaacgtc gccaacagag gttaaacagg tagtgactat
    11881 ggatatggat ctaaacaatc gcctgactga agatgaaacg cttgagcagg cctacgatat
    11941 ttttctcgaa ctggctgccg acaatctcga cccagctgat gttctgctgt tcaatcttca
    12001 gtttgaagag cgcggcggcg cggagttatt cgatccggcg gaagactggc aggaacatgt
    12061 tgatttcgat ttgaacccgg acttttttgc cgaagtggtg attggtctgg cggacagtga
    12121 ggatggtgaa atcaacgatg ttttcgcccg cattctgtta tgccgcgaaa aagatcacaa
    12181 actttgccat atcatctggc gggaataaat aatcgctttc ggcgtgaata agggctgaca
    12241 actgtcagcc cttattgtgt ttaaagcgga tcgactttca ggcaagaaac ggcgtgtctg
    12301 aaactccctt ccagcacagg acgtgttttg gcgcactccg gaccggcaat cgggcaacgg
    12361 gtacggaaaa cacaaccgga aggcgggttg atcggcgacg gtaattcccc ttccagtaac
    12421 tggatggttt tgttcttctc cagatccgga tcaggtatgg ggactgccga catcaatgcc
    12481 ctggtgtaag gatgtagtgg attgtggtag acctcatcat aggtccccag ttctaccgca
    12541 tggccgagat acatcaccaa cacacgatcg gaaatgtgtt ttaccacggc caggtcatga
    12601 gcaataaaaa ttaatgacaa tcccatctca cgttgcagct gctggagcag gttgaccacc
    12661 tgcgcctgaa ttgacacgtc cagcgccgac accggctcat cacagataat cagcttcggt
    12721 tcaagaataa gagcacgagc aatcccgata cgctggcact gcccaccgga gaactcatgc
    12781 ggatagcggt taatcaggtt aggcaataac ccgactttca gcatcatcgc cttcacgcgc
    12841 tcgcgaactt cctgacgtga cattttcgga tgataagtac gcagtggttc agcgatgatc
    12901 tcgccgatgg tcatgcgcgg gtttagcgat gccaacggat cctggaaaat catctgaata
    12961 tcactgcgaa cggcacgcca ttcatcgggc ttaatgccca gcaactcttt acctaaccag
    13021 gcaacatgac cgtcggtcgc tttaaccagg ccaatgatgg cgcgagcaaa ggtggactta
    13081 ccgcatcccg attcccctac cacacctaat gtttcccctt catacaggcg aagcgtgaca
    13141 ccatcgacgg ctttgagcgt tttcggcggt tgccagaacc actgtttgcc atctttgatt
    13201 tcaaagtgca ctttcagatc ggcgatttca aggaggactt ttcttccttc ggttacagca
    13261 ttcataacag ttcctccacc ggtttaaagc aagcacacag atggccaggc gtaaactctt
    13321 ccagcggcgg tgcgctacta caaatttcca tcgcatgcgg acaacgtggc tggaacgggc
    13381 aacctttcgg taatcgcagc aggtttggcg gattacccgg gatggtcaac attgtttcac
    13441 cttccgcatc gagacgcggc accgcgttga gcaaaccgat agaataagga tgaacgggtt
    13501 gataaaagac atcgcgtgcg ttgccatatt ccatcgtgcg cccggcgtac attaccagca
    13561 ctttgtcaca gatccccgcc accacgccaa gatcgtgggt aatcataatg atggcggtat
    13621 taaattcccg tttcagttca tttaatagcg tcatgatctg cgcctgtacg gtgacgtcca
    13681 gcgctgtagt gggttcatcc gcaatcagca gcttaggtcg acatagcaat gccatcgcaa
    13741 tcataactcg ctgacgcatg ccaccagaaa actcgtgcgg gtacattttc atgcgtttac
    13801 gtgcttccgg catttttacc gcatcgagca tccgcaccga ctcttcaaac gcctcagctt
    13861 tgctcatgtt cttatgcagc atcagcactt ccatcaactg ctcaccgacg cgcatatagg
    13921 gattcaacga agtcattggg tcctgaaaaa tcattgagat ttgttcagcg cgcagtttat
    13981 tgagttcatg ctctggcaaa ttgaggattt cacgcccatt gaaggttgcc gatccgccaa
    14041 tacgtccgtt ggcagccaac aggcccatca acgcaaatgc agtttgcgat ttacccgaac
    14101 cagactcacc tacaataccc agcgtttctc cggcacgtag ggaaaaattc aaatcattga
    14161 ccgccgtgac gtcgccgtcc ggggtactaa aggtgacacg caaatctttc acgttcagca
    14221 gtgcgccagc ctgttgttgt gcgagcggca cagttgcagt ttcaattacg ctcatggctg
    14281 cactccttaa cgatctttcg ggtcgagggc atcacgcaag ccatcgccga taaagttgaa
    14341 acaaaacagc gtcaccacga ggaatcccgc tgggaacaac aataaccatg gagagacttc
    14401 catcgagttc gcgccatcac tcagcaatgc accccagctg cttaacggct cttgcgtacc
    14461 caaccccagg aagctaagga aagattcaaa gaggatcatg ctgggcacca gaagtgatgc
    14521 gtagaccacc accacaccga gtacattcgg cacaatgtgg ctaataacaa tgcccgatgt
    14581 cgatacaccg ccaacttgtg ccgcctcaat aaactctttg cgcttcagac tcagggtttg
    14641 cccacgcaca atacgagcca tatccagcca ggaaaccatg cctatcgcca cgaaaatcag
    14701 caggatgttt tgaccgaaaa aggtcaccag caaaatgacg aagaacatga atgggaagga
    14761 gttgaggatt tccagtagac gcatcattac cgaatccact ttaccgccca gataaccgga
    14821 aagcgaacca taaagtgtcc cgacgaccac agccaccagt gccgcagcaa caccgaccat
    14881 gagtgagata cgcccaccaa tcgcaacgcg cacaagcagg tcgcgaccgg atgagtcagt
    14941 accaaagtag tgaccggact ccatatccgg ggtgctggac atcatcgccc agtcagtatc
    15001 gtcataggca aactgggaaa gcatcggtgc caggattaca aataacgcga tcagcaccag
    15061 caccatcaga ctggcaaccg ccgcacggtt atgaataaaa cgtcgacgtg cgtcctgcca
    15121 caagctgcgc ccttcgacct ccagcttttc actgaaattt tccagcgtct cgctgttttt
    15181 cttacttaac atcatcgcga gctccagtat cagtaacgga ttttcgggtc gataaccgca
    15241 tatagcacat cgacaatggc attaaacaaa atggttaaag caccaaccag gatggtcagg
    15301 cttaacacta aggaatagtc acggttcaat gcgccattaa cgaacagttg tccaatcccc
    15361 ggcaaaccat aaatggtttc gataaccata gaaccggtaa taatgccgac aaatgcgggg
    15421 cccatataag agagcacggg taacagagca ggttttaatg cgtgacgtaa aatgatccgc
    15481 cgtataggta accctttcgc ccgggcagta cgaataaaat tggagtgtaa tacttcaatc
    15541 atagagccac gggtaatacg cgcaatactg gcgatataag cgagtgacaa cgccaccatc
    15601 ggcaatatca tgaatttaag cgccccacca ttccagccac cgcccggcag ccaatgcaaa
    15661 atgatcgcaa atatcatgac taataatggt gcaaccacaa aactggggat aacaaccccg
    15721 gtcattgcca gccccatcac ggtatagtcc catttggtgt tttgttttaa tgcggcaata
    15781 acgccagcac taacacccag tattaccgca aggaaaaatg ctgcggctcc cagtttggca
    15841 gaaacgggaa aactggatgc aaccaagtca ttgaccgaat aatctttata tttaaacgat
    15901 ggaccgaaat caccgtgcgc cagttgtttc aggtagctga aatactgtgt catgatcgga
    15961 tcattaagat gatatttcgc ttcgatattg gccatcactt ctggcggtaa agtacgttcg
    16021 ccggtaaaag ggcttcccgg cgcgaggcgc atcataaaga acgaaatagt aataagaata
    16081 aatagcgtcg gaatcgcttc cagacagcga cgtagaataa attttaacat tgccctacct
    16141 tctggcctgt gccttctgta atgcgataaa aatcagacac cgtggagcag gacactcctg
    16201 ccccacgtat tgccattagt gcttcacaat gtacatattc cgggtatagg tattatccag
    16261 cggatctttg ccggtatagc caccaaccca cggtttcacc agacgcgcat tcacgtagta
    16321 ataaacagga acaatggccg aatccttatc cagctgttgt tctgctttag tgtacagagc
    16381 tgtgcgctgc gcctcgtcag tcactttcag cgtttccgcc ataatgctgt caaaggccgg
    16441 gctcttataa tgcgcggtat tcatcgagct gttcgaaagc atggtgttca ggaaggaagt
    16501 tggttcgttg tagtcagcac accagcctgc acgggccaca tcaaaagtac cctggtgacg
    16561 ggtgtcgagg aacgttttcc actcctggtt aaccagtttg acgtttacac caatgttttt
    16621 cttccacaat gaagaggcag caatcgccag ctttttatgc agatcggagg tgttatacaa
    16681 caggttgatg gtcaacggtt tgtctgcggt ataacccgct tcagccagca gttttttcgc
    16741 ttcttcgtta cgtttttcct ggctccagcc aaaccattcc ggctgagtca attttgcgcc
    16801 atcagtatac ggtggagtgt aaccataggc gggcatgtcg ccctgcgctt tcactttatt
    16861 aacaatgata tcgcggtcca tacctagttt cagtgcggta cgcacacgca catcgttgaa
    16921 tggcggtttc tggttgttaa tttcgtaata gtaagtgcac aggtatggat caacgtgaac
    16981 ttcgtccggg atctcttttt tcagcttctg gaacaattcg atcggcatgt tgttataagt
    17041 catgtcgatt tcaccactac ggtagcggtt gacatcggta acttcagaag caataggcaa
    17101 ataggttacc tggttaataa cggttttcgc gttgttccag taggtcgggc tgcgttcaag
    17161 aacgattcgt tcgtttacga cccaatcttt taaggtatag gcaccgttgg tgacgatatt
    17221 accaggctgg gtccattttt cgccgaattt ctcgatagcg gcttttggca ccggtgaagt
    17281 tgatgggtga acaagtaatt tatagaagta cggaacgggt tcacttaagg tgacttctaa
    17341 tgtgtgatca tcaatagctt tcacgccgag atcggtaatc ggttttttcc cttcaagaat
    17401 ttcatcaata ccggcgatat gcccatattg cagataactg gcatacggag aagcagtgtt
    17461 cggatcaaca gaacgttgcc agctatacac aaagtcttgt gctgtgactg gcgtaccatc
    17521 agaccatttc gcatctttac gcaaatggaa ggtccagact ttcgcgtctt tattatccca
    17581 ggattcagcg acgccaggtg ccggatgacc gtcaagatcg ctgaccagta agccttcaaa
    17641 caggtctcgg ctgatattag actccggaac accttcaatt ttgtgcggat ctaatgactg
    17701 aacttctgaa ccattgttac gtaccagcgt ttgtttttcc gccagtgtga cgcctgcggg
    17761 tacatcagct gccagcgcga cattccctgc cattagcgca gccagaacgc cagctgctac
    17821 taaacttctc ttggtgatgt tggtcattgt tttttggact ccctcattat aattactggt
    17881 attaaccagc atgtgtaatc ccggattggg accctgcaca ggctcggaga ctttctgctg
    17941 tcaggttact ttatcgttac ggattgctat caccgacttt atttattctg gtgattcaga
    18001 cgatcacctt atgtcgattc gtactgtgtc tcccctgtcg agacacatta ttctttcgcg
    18061 acgctcttta attgttaatt attctcatta ccaataaaga atcgtctggc ggttgcccgg
    18121 aaagtaccaa atgcggttct tactcgccaa tacattttgc aaaattgtta agcaattctc
    18181 ttttattgac cgtatcagaa tctatagctt gcatccatgc cttaacaaac atctttcaat
    18241 tcaatcaaat agagattggc taagcatgtc tatcttttct gattacagtt tcagtattga
    18301 aatgcacaat aatttaacaa ttgattatta tttagcactt tcctctacca aaggcagtgg
    18361 aattactaat atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat
    18421 aacatgatgg tgaggagaag cagttaagaa aatccttata actaactgta atttgagaag
    18481 aatattttta cctattcctt ttgcttttga taactgcg
//
LOCUS       DAJTMK010000078        18286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTMK010000078 DAJTMK010000000
VERSION     DAJTMK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18286
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.079"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..371)
                     /locus_tag="OO135_002244"
     CDS             complement(<1..371)
                     /locus_tag="OO135_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5398077.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLMADFTGELHRLIS
                     EYLQCKRRKDKSVEPAPLYYLWSREAVANDTTPYLMVLLLNNDVWCKSREVVALRNLI
                     EQAWGKVLGEDNSITHLVVYP"
     gene            complement(465..1079)
                     /locus_tag="OO135_002245"
     CDS             complement(465..1079)
                     /locus_tag="OO135_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5398078.1"
                     /translation="MITIETLYGECHEAHGLKIRNQVNNLFQKHPRITAIRVDLHYPP
                     VIDNGDTITCFPNLSPGEISRFRNSLNAKINADQHRKKQQGKRTYATSVCIIWAKEYT
                     QSFKCHYHVCLFFNKDAYYHLGDYERSDTLRGLITGAWYSALGLELEDHQGLVHFPAN
                     GRYILDINDPDFPEQYQRLLARLDYLTKLDTKASGQRNFGYSGF"
     gene            complement(1461..1961)
                     /locus_tag="OO135_002246"
     CDS             complement(1461..1961)
                     /locus_tag="OO135_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5398079.1"
                     /translation="MIQRYFKEPLLQLTGELGRPDYTLADRKRLVRLLTQDMQRGDIL
                     ITKSLTCFGNTPEKIQEVLSLSIRQGINICFFTSSELPDGDDIPVVLTGMILPTDIRG
                     KIPDISIRKIKRKPGRKEGSIHSKNIRQLKLQGFSQSQTARKLSISLSTVKRHWDKGI
                     VDLTAE"
     gene            complement(2243..2989)
                     /locus_tag="OO135_002247"
     CDS             complement(2243..2989)
                     /locus_tag="OO135_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398080.1"
                     /translation="MMKRVSFSLAETYEADVIKKYQHLKKCSFSAAIKECLKLGAPVL
                     NRINENIAAITDIEDKLRQFFNEEPFVQRTRPEITKGEFFHSIYKSHIKYEYDVLDRK
                     IFPHESTRNAMGVAEKKGIKENATLMLEYYKVEKAICIYTNRKVSHTLNRAGGFYKTI
                     LIKTSVFGDYFFDFCNSVCLPIDELIEYGTKETVRRHQIRSTGFCTFHIPIFYINNKA
                     VIVPVLRTEEVSQSSRTGGDVIIINPFEDE"
     gene            complement(3052..3279)
                     /locus_tag="OO135_002248"
     CDS             complement(3052..3279)
                     /locus_tag="OO135_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5398081.1"
                     /translation="MWKPLRDENRLSILKIYGYTCDRLVREKERQKITSISRSQAWKL
                     EREGKFPPRKTIGKKSCGWLLSDLLWWISQY"
     gene            4060..5664
                     /locus_tag="OO135_002249"
     CDS             4060..5664
                     /locus_tag="OO135_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398082.1"
                     /translation="MVTKKVKTNPPYYRVLSGTSMPFPHIHEAVSEQLLLLIFKARRL
                     YLDPEYRPWFDEQHRILSALRANTKLQSILEKNRENYSISETDNPLSKDLYAAFQVMC
                     HLYNGFDNRLVSLKTWGDMNREPTAKFVGQNSGGWKTPEMEEFVRNDLFNILTDCKAH
                     CSPLPALVRQGYCHQDLSVAHSCTKELLEQLNRYHQIDESAILARTLGLAINNCYHSE
                     TEIPEWLSPFENEYKSPWNCEKPDTNDVSKLRKQLLTIGRTEFVDKMFSLANKNATKI
                     IYDGYVKLYALEIYEWLNKLVQYFKGNDFIRYTWKFTTIISDESDTIENTTAWEKDYC
                     QITIQRDELLKLIYTNTLQDAAMNLADCLQQAYTEPLKMEINYPGINIDAKERKIRKS
                     IEFRSELNNFTNIMKQLEIFEKWYNANKILLVSNKKIRTEKIDVNIRLAGLKGYDLNE
                     GIPDGQGRRIKDGVNELVKADGSLQLPQDISDTSLNRYRQAVQHIIKDEIDTLLIAQR
                     EKNKINPYSEDRYSIRPLWGKCLVEQ"
     gene            complement(5747..6343)
                     /locus_tag="OO135_002250"
     CDS             complement(5747..6343)
                     /locus_tag="OO135_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398083.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDVRHKVVATLELQRRPRLEGVFVQKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETIVTKAGVVLLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGE
                     RISYDGKSIPESEIWSEHPERNRHAVVLVAESVNGKAA"
     gene            complement(6473..7789)
                     /locus_tag="OO135_002251"
     CDS             complement(6473..7789)
                     /locus_tag="OO135_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5398084.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQPSGSK
                     IFYFRYYVEKGKKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQAELERQQFAE
                     QQRIQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGITFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRFCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRAFNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(8086..8850)
                     /gene="nfeF"
                     /locus_tag="OO135_002252"
     CDS             complement(8086..8850)
                     /gene="nfeF"
                     /locus_tag="OO135_002252"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR5398085.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRSFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            9138..9761
                     /gene="nfeR"
                     /locus_tag="OO135_002253"
                     /gene_synonym="yqjI"
     CDS             9138..9761
                     /gene="nfeR"
                     /locus_tag="OO135_002253"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5398086.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(9915..11435)
                     /gene="aer"
                     /locus_tag="OO135_002254"
     CDS             complement(9915..11435)
                     /gene="aer"
                     /locus_tag="OO135_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5398087.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQGLQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLEVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            11852..13231
                     /gene="ygjG"
                     /locus_tag="OO135_002255"
     CDS             11852..13231
                     /gene="ygjG"
                     /locus_tag="OO135_002255"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5398088.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVVAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(13273..13605)
                     /locus_tag="OO135_002256"
     CDS             complement(13273..13605)
                     /locus_tag="OO135_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR5398089.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            13824..14807
                     /gene="ebgR"
                     /locus_tag="OO135_002257"
     CDS             13824..14807
                     /gene="ebgR"
                     /locus_tag="OO135_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5398090.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            14991..18083
                     /gene="ebgA"
                     /locus_tag="OO135_002258"
     CDS             14991..18083
                     /gene="ebgA"
                     /locus_tag="OO135_002258"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5398091.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYQHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            18080..>18286
                     /locus_tag="OO135_002259"
     CDS             18080..>18286
                     /locus_tag="OO135_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5398092.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVH"
BASE COUNT         5018 a         4500 c         4078 g         4690 t
ORIGIN      
        1 tcaggataca caaccagatg tgtaatggag ttatcttcac ccaatacttt tccccacgcc
       61 tgctcaatca gatttctgag agccaccacc tcacgactct tacaccagac atcgttatta
      121 agtagcagta ccataagata aggagtggta tcgttagcca cagcttccct actccagaga
      181 taatataaag gggcgggctc aacagattta tctttacgtc gcttgcactg caaatattca
      241 gaaattagtc tatgcagttc accagtaaaa tcagccatca gagagggaat tgccttgttc
      301 atacctgggc aaggtattaa tttaaattgt aataatttaa ttttaggatg tgtagctgca
      361 gcccgataca gagttgcaag gacatacttt tgccagaggg cgttacagga aagcttaacg
      421 tttgattctg tatacataat aaatcacctt acagttacaa taggtcaaaa accgctgtag
      481 ccaaagttac gctggcctga tgctttagtg tcgagcttcg tcagataatc cagacgtgcc
      541 aataagcgct gatactgctc agggaaatca ggatcgttaa tatccaggat gtaacgtcca
      601 ttagcaggga aatgaactaa tccctggtga tcttccagtt ccaaccctaa agcactgtac
      661 caggctccgg taattaaccc tctcagggtg tcagagcgct catagtctcc caggtggtaa
      721 taggcgtctt tgttaaaaaa aaggcagacg tgataatgac atttaaagga ctgcgtatat
      781 tctttcgccc agataataca aaccgacgtt gcgtaggtgc gtttgccttg ttgtttttta
      841 cggtgttgat ctgcattaat tttcgcattc agcgagttac ggaaacgaga tatctctccg
      901 ggactcaggt taggaaaaca ggtaatggta tccccgttgt caatcacggg aggataatgg
      961 agatccaccc gtattgccgt tattctggga tgtttctgga agaggttatt cacctggttt
     1021 cttattttta acccgtgagc ctcatgacat tcaccgtaaa gagtttcaat cgtaatcatg
     1081 ttaatattcc ttttgctttc agagataagc taatagttaa cctacccaat acacaacaga
     1141 agcagatatt aatattcgta tcagtagttc ccagagggat atataaccct ctgggcgtca
     1201 attaagaaaa acgctactgc ctattgttac attagagaac aggatgatgt tacacagtta
     1261 cattagaacg ttctaaaaca ttaaacagag ttatgatcta cacacaacta attatttggt
     1321 atcagaatac cattagctct attagtacat catatccatc attacacata tactatacat
     1381 cacagatata atatatatta cctatccaac gcacatgtat ttataactcc attgccaata
     1441 gctacaagga ctataaaatg ttactctgca gttagatcaa caataccttt atcccagtgt
     1501 ctttttacag tggataaact gatgcttaat tttctggctg tttgtgactg actgaacccc
     1561 tgcaatttca actgtctgat atttttacta tgaatactcc cttctttcct tcctggtttt
     1621 cttttaattt tccgtatact gatgtcggga atttttcctc taatatctgt aggtaaaatc
     1681 atcccggtga gtaccacagg aatatcatca ccatccggca actctgatga cgtaaaaaag
     1741 caaatattaa taccttgtct gatacttaaa gataaaacct cctgtatttt ttccggcgta
     1801 ttaccaaaac aagttaacga tttagtaatc agaatatcac ccctttgcat atcctgcgtc
     1861 aggagtcgaa ctaaccgttt tctgtctgcc agagtataat ccgggcgacc caattcgcct
     1921 gttaactgca atagtggttc tttaaaatat ctctgaatca tggattttaa gcgattaagt
     1981 tttgaccatt tttcagagtc tatgtatgca aaactcgcca taatgcctca aactggtgtc
     2041 atgaattaat tattgaacct gttttttcct gagtcgcatt actcttttct aagggaggct
     2101 ctccagatac atcggaaaag tactcgataa aagagttggc cactcccggg taatgattgc
     2161 tctctttaac ctcagaaagc ggtttctgtt catcatttac accacgattt ctgggttatg
     2221 aataaatcta cgcgggccat aatcattcat cttcaaatgg gttaataata ataacatctc
     2281 cacctgttct ggaactttgt gaaacttcct ctgttctcag aacaggcact attacagcct
     2341 tattattgat ataaaaaatc ggaatatgaa aagtacaaaa tcctgtacta cgaatctgat
     2401 gtcgcctcac tgtttctttc gtaccatact caatcaactc atctatcggc aggcaaactg
     2461 aattgcaaaa gtcaaaaaaa taatccccaa aaacactggt tttaatcaga atggttttat
     2521 aaaaccctcc tgcacgattc agggtatggg aaacttttct gttcgtgtaa atacaaattg
     2581 ccttctcaac tttataatat tccagcatta aggtcgcatt ctctttaatt ccctttttct
     2641 ctgcaactcc catagcattt ctggtgcttt catgaggaaa tatctttcta tcgagaacat
     2701 catattcata tttgatatgc gatttataaa tgctatgaaa aaactctcct tttgttattt
     2761 caggccttgt ccgttgcaca aatggttcct cattaaaaaa ctgacgtagc ttatcctcta
     2821 tatcagttat agcagcaata ttttcattta tcctgtttaa aacaggagcc cctagcttta
     2881 aacactcttt aatcgctgct gaaaatgagc atttttttaa atgctgatac tttttaatta
     2941 catcagcttc atatgtttct gcaagagaga aagagacacg tttcatcatg aaactccttt
     3001 ttaattataa aattaaaata tagaaatata tattattctg atactataag gttaatactg
     3061 actaatccac cacaatagat cacttaataa ccacccacag gatttctttc caatagtttt
     3121 acgtggcggg aattttcctt cacgttccag tttccaggct tgtgaacgag aaatagacgt
     3181 aattttctgg cgttcctttt ctcgtaccag tctgtcacag gtgtatccat agatcttcaa
     3241 aattgataaa cggttctcat ctcttaacgg cttccacata ttgctctcct tatatattca
     3301 tgattttcag attgtatatg catgtgagct gctcaaatca ctacatctat ctgtagtggt
     3361 atgctgttga acagcaccag caactcacga gcaaaacaat acatttgcac aaccaagaaa
     3421 tcaaattgcg agaaatgatt taattaactg acttctaata acacaataac aacaaagaaa
     3481 gtcacaatat aaaaataact caaacaatgc tttttggcat acaaaataaa aatcaaaatc
     3541 acaatattcg taggttattt tatgcagaca aaaacaaaaa aaaatcttgt tataatctat
     3601 gatatcaaga ggaggggtag tgaccaaaaa ttctccccag aaaagccccc acaacattag
     3661 tttgaaaacg tgacgaagtt cacaaaatta tcttctggcg ataatagaac ttgtaagagg
     3721 gagttgattt ttgccaactt tctaaagcta aagtgcagac aaaagataat aattgaatga
     3781 aaacgataca tattttcaga tgaaagtgac gtgactgtag agggctataa ccgtaaaatc
     3841 atttgcgtcc tgagcaatat gcaacaaaag ggaatgctcg taaaaaaatc aggaataaac
     3901 gctaaggata tcgtattaga ttcagcatcg tataatgcca ctgcataact gattcccatt
     3961 tcgtagaggc tgttaactct gtatcaatgt cctgacaaag gactgtatca agtttctcta
     4021 ctggaaacag ctatgcaata gacggaagaa tttaacatta tggtaactaa aaaggtaaaa
     4081 acaaaccccc cgtactatcg tgttttatcc ggcacctcca tgccgtttcc ccacatacat
     4141 gaagctgttt ccgaacagtt gcttctactc attttcaaag cacgcagatt gtatctggat
     4201 ccagaatatc gtccttggtt tgatgaacaa catcggattc tctcggcatt acgcgctaac
     4261 acaaaactcc aaagtatttt agagaaaaac agagagaact actccataag tgagaccgat
     4321 aatccactat ctaaagatct gtatgctgct tttcaggtta tgtgccattt atacaatggt
     4381 tttgataaca gactcgtcag cctgaaaacc tggggagata tgaacaggga accaacagca
     4441 aaatttgtcg gccaaaatag tggtggctgg aaaacacctg agatggaaga attcgtcaga
     4501 aacgatttgt tcaatattct gacagattgt aaagctcatt gctcaccact tcccgctctg
     4561 gttcgacaag gatattgcca tcaggatctt tctgttgcgc actcatgtac taaagaatta
     4621 ctggaacaac tgaaccgata ccatcaaatt gatgaatcag cgatacttgc cagaaccctt
     4681 ggtttggcta ttaacaactg ctatcactca gaaacagaga ttcctgaatg gctttctcct
     4741 tttgaaaatg aatacaaaag tccctggaat tgtgagaaac cagatactaa cgatgtgagt
     4801 aaacttagaa aacaattgct aacaatcggg cgtacagaat tcgtggacaa aatgttttcg
     4861 ctagccaaca aaaatgcaac aaagataata tatgatggat acgttaaact ttatgctctg
     4921 gaaatatatg aatggttaaa taaattagtg caatatttta aaggaaatga ttttattcgt
     4981 tacacctgga agttcacaac aattatttcc gatgaaagtg acacgattga aaacaccacc
     5041 gcatgggaaa aagactattg tcaaatcact atacagcgtg atgaacttct gaaactgatt
     5101 tataccaata ccctgcagga tgcagcaatg aatcttgctg attgcctgca acaggcatac
     5161 acagaaccgt taaaaatgga aataaattat ccaggaataa acattgatgc aaaagagcgt
     5221 aaaattagaa aatctattga attcaggagc gaactgaata acttcacaaa catcatgaaa
     5281 caactggaaa tatttgaaaa atggtataac gccaataaaa ttctgttggt aagtaacaaa
     5341 aagatacgta cagaaaagat tgacgtgaat attcgtcttg caggactgaa aggctatgat
     5401 ctgaatgaag gtatacctga tggtcaggga cgcagaatta aagatggtgt gaatgaactt
     5461 gtcaaggcag atggcagtct acaacttcca caagatatca gtgacacttc gcttaaccgt
     5521 taccgtcaag cagttcaaca cattattaag gatgagatcg acacgttgct gatagcacag
     5581 agggaaaaaa ataaaataaa tccatatagc gaagatagat actcgatacg acctttatgg
     5641 ggaaaatgtc tggtcgaaca gtaactcact gagaaagggg atagcagttc gctatcccca
     5701 aaggagactt tccagcaaga tctcaaaaaa tgagacactc agcaaattat gcggctttac
     5761 cattaacaga ttcagccaca agaacgacag cgtgtctgtt tcgctcagga tgttcactcc
     5821 agatttctga ttccgggatg cttttcccgt cataactgat ccgttcgcca tcaaacgggt
     5881 aataccgtgc ggaatcagta tctaaaataa acaccttcag attcgttgaa cgacgcgcaa
     5941 tatgctgcca gagagcttta cctccttcat attgctcgtt atcacttaat agtacaacgc
     6001 ccgcttttgt gactatcgtt tcataaacaa acgttgcaag accaacatca cgtacagcgc
     6061 catcgacaac aatcccttca atctgagcaa catcctgttc ttcccagaat ttctggacaa
     6121 acacgccttc aagccgggga cgacgctgaa gctccagagt ggcaacaacc ttatggcgaa
     6181 catcaccatc aataacagaa ggtgcagctt cgattaccgc agcaaacgga gtctttggat
     6241 cccagatgat ctggtaaagc acgccctgaa tagttactcc atccagtatc gaaaaacgac
     6301 gagctacagt agcaggggag taagacattg gaattacagc catttttgaa tcccctctga
     6361 gtaataccgg cacgaaaatt tacctcaata attatagtaa acaatacaag aaccaagtgc
     6421 atcaacaata cccatcaatt gtacacaatt cgtacagcaa ccaggaactc cctcaccttc
     6481 tgccaaacag attgacaaca ttattgcctg tttgctgttg atagttatta acccggtcct
     6541 cccagatctc aagtgctctg cgtttttctg gcaaataatc gtatcgatca taatgttttg
     6601 aactgacatc gttcaacgca tgattttgaa tacgatcgcg gatttcctta ctgatcccag
     6661 cttctcccat taacgtttta caggtccgac gtaagtcacg tgctgtaaaa attttaaagt
     6721 caggattaaa agcccggaaa tacatgatag cgcgagccag actgtccgta cgaaccggac
     6781 gcttaccatt agtcgaaagt ggaaaaatgt aagggctgga tttttctttt gttaactcct
     6841 gcacacagga taattcctgt aatgctgatt cagtcaacgg tatcaaatgc tctcgtttat
     6901 ttttagagac atcagcagtc accagcagcg ttttttgttg aaaatcaaca tcacaccact
     6961 gactggcgat catttcgaaa ggacgttgcc ctccggtata gatacagaat cggatcaaat
     7021 gttgcatcaa taatccaacg ttggttgctt cagcaaaatg ttccattaga aaacgcaact
     7081 cttcaagtgt cagccaggta tcaccgactt tttcagcaga tgactgtttc ggtatcactg
     7141 acaccggatt tgcctccagc ccaaaagtaa tccctacact tgtgttcatc ggatcgttat
     7201 ctgctttcag tccatagtta aatgcagcca tcaggtaaga acggatccgg ttagagtgaa
     7261 ccacagcacc gcgctgaatg atcccggcca ggatatgttt aatctgtccg gaagtcacat
     7321 ctttagcttt agtttcacgg gggatcactg tatagcattc attttcaagg cgtttcagaa
     7381 catcctgcca tgtcctctta ttatcaattt tcattttgtt aacataacca tgaatcaatg
     7441 cttcaaatga gccctgtgat tgatggagtt gtatccgctg ctgttctgca aactgttgtc
     7501 gttccagttc tgcctgggga tcctttcctt caatgagcca ggcaccatac ttttttgcca
     7561 gttcattcgc tgtggccagt ttcatctcag gccatatacc caattgaatg aatctttctt
     7621 ttttcccttt ttccacgtaa tagcggaaat agaaaatctt gctgccagaa ggctgaactt
     7681 tgacaccaag tcgtccagtg ccacgttgag agctgttgct ccagacatag aacgatgtct
     7741 tttttgcttt caatgctcgg atggctgttt cagtaagatt gacggccata gaacctcttt
     7801 tctaaaccag taacaatcag aataactcgt gattttgggt caggtaatgg gtcaggtaac
     7861 gatcgtacaa tgaagaaaaa acggaaccaa gcggagacag ggaaaatcac ataatttatt
     7921 gattacaaac aactaataaa gcaatcacga acaaacaaaa acaatataga acgaacttgc
     7981 ctcatgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac tagatatagc
     8041 aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact ttgcgtgcca
     8101 gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc ggcggcttaa
     8161 attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg cagggatttg
     8221 catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt cgatattaaa
     8281 accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgtacgctaa ccagcgcact
     8341 aacttgcggt ttaacggcaa gtttgctcaa cgtttccagg cggcggcgca gtgcgggcat
     8401 tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca ccagcgaacc
     8461 gcgcggacca gccaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc agccgctggc
     8521 gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt cgtcatacag
     8581 cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc cctcttccgt
     8641 taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc tgtgatcgtc
     8701 aaagctgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc gctgaaaacc
     8761 ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat cattgcgaac
     8821 gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg taatcagata
     8881 tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc aagcgattag
     8941 tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc atcattactc
     9001 aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt ccacatttaa
     9061 aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata tatctaatta
     9121 agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat gaaggccagc
     9181 cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc ggacacggtc
     9241 accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc ggtcgtcggc
     9301 aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc tcgcgcgatg
     9361 acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg aattacaccc
     9421 caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg ctgattacca
     9481 tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc gcgcagtggc
     9541 tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg cgttgcgttg
     9601 gcgcggcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt aaagcggtgc
     9661 tggatttacg cgtcaaccag agcgatatca gtgatgcaca aataaaaaag atcattgcgg
     9721 tgatcgaccg cgccgctttt gatattacgc aactggatta atcgccgcat ccgccagtgg
     9781 cgcggtgcaa ttgccggatg cgacgcttga cgcgccttat ccggcctaca cccgctacac
     9841 accccgcagg cctgataaga tgcgccagca tcgcatcagg cattgtgctc caaccgccgg
     9901 atccggcata ccgattaatg cagcaccgtc accgcgtctt ccagtcggct ggcgcggtgt
     9961 ttcaccatcg ccgacacctg cgcactctct tccaccagct cagcattttt ctgggtgatc
    10021 aggttaagct catccactgc acgggtcagg ctggaaagcc catcggcctg ttccagcgtt
    10081 gaatggctaa tctgggcgat caactgggtg acgtttttca cctgtgccac aatatcttcc
    10141 atcgtccgtc cggcggcgtg tacctgctgc gaaccggatt gcaccttatc agcactggca
    10201 tcaatcagct tgcgaatatc gttggcagca ttagcgctgc ggctggctaa atgacgcact
    10261 tcccctgcca ccactgcaaa acctttgccc tgttcgccgg cacgcgccgc ttccaccgcc
    10321 gcattcaggg ccagaatatt ggtctgaaac gcaatatcgt taatcagcga agtaatggtg
    10381 ccaatgcgct gggtactgtc ggcgatatcg tccattgtct tgatcaccgt ggtcatcgcc
    10441 tccccaccct gcactgccgc attactggcg gtgatagaaa gtttatcggc agccgacgcc
    10501 gtggcagagt tctgtttcac cgacgccgcc atttggttca tggtcgccac cgtttgctga
    10561 acgttatcaa ctgtctgctg ggtatgttcg ttcagttcat cggtgccttt cgccagcgtc
    10621 tcactgccat ttctgacact ggacacctgg cttgagacat cgttaattag ccaacggcac
    10681 atcaggccaa gctgccctac cgcgcgtaat gtcagtccca gctcatcgct gcgattcagg
    10741 tgctcaacac tattacgctc tccggtcgcc accttcagtg cctgacgggc gacattttct
    10801 atcgggcgca caatctgcca ttcaaaacaa gcgcttgcca acaataccac taacacacag
    10861 aggaaatacg tcaccaccgg ggcagcaaca aaccaaagca tggccgccag caagataaac
    10921 atcagggtca tcactccacg cgcccgccag cgaagcggta atgaaggcag tttacccagc
    10981 cagcctttac gcacctccag gcctttatga atacgcttac tggtacgtcc ggcgttcagc
    11041 gctttgtaca gcggctccac cgccgcaatc tcttcatccg tcgcccgggt acgaatcgac
    11101 atatagccac tgatttttcc ctcgcgcacc atcggtaccg cattggcccg tacccaataa
    11161 tggtcgccat ttttgcggcg atttttcacg atgccgctcc agggctcccc ttttttcagg
    11221 gtgaaccaca tatccgcaaa cgccgctttt ggcatatccg ggtgacgcac catgttgtgc
    11281 ggctgccctt gtaacccttg caaggtaaag ccgctcacct gcacaaaagt gtcattagca
    11341 tgagtgatat agctttgcag atcggtagtg gacatcagag tggtatcgtc cgccagcggg
    11401 gtattttgct gggtgacata cggatgagaa gacatgttcg cgtcctatgc aggttatatg
    11461 gttgttaact tcttgtcaga gtttatgtcg gccccgctgc ggttatcttt aaccgattaa
    11521 tttgatttag atcgcaattt gcgattaaag cacaaatcta attccttgat ttaaaatact
    11581 ttcactctgt tactatacga aaacgttaat tatcttgccc aaaatcaggc aattatcgcc
    11641 ctgaaaacgt gcatttgcac agcaatcatc aaatccatac ccgacaaaaa ccgtgcaaaa
    11701 taacaacgaa tgttaacaaa tagcattaaa tactgcgcaa atgataacca catttgtgtt
    11761 tatcccgatt ttcgcgatcg cagccggagt ggcgcaatcc ctgcaatact taaatcggta
    11821 tcatgtgata cgcgagcctc cggagcatat tttgaacagg ttaccttcga gcgcatcggc
    11881 tttagcgtgc agcgcccacg ccctgaatct cattgagaag cgaacgctgg atcatgagga
    11941 gatgaaagca cttaaccgag aggtgattga atacttcaaa gagcatgtca atccggggtt
    12001 tttagagtat cgcaaatctg ttaccgccgg cggggattac ggagccgtag agtggcaagc
    12061 gggaagttta aatacgcttg tcgacaccca gggacaggag tttatcgact gcctgggagg
    12121 ttttggaatt ttcaacgtgg ggcaccgtaa tccagttgtg gtttccgccg tacagaatca
    12181 acttgcgaaa caaccgctgc acagccagga gctgctcgat ccgttacggg cgatgttggc
    12241 gaaaaccctt gctgcgctaa cgcccggtaa actgaaatac agcttcttct gtaatagcgg
    12301 caccgagtcc gttgaagcag cgctgaagct ggcgaaagct taccagtcac cgcgcggcaa
    12361 gtttactttt attgccacca gcggcgcgtt ccacggtaaa tcacttggcg cgctgtcggc
    12421 cacggcgaaa tcgaccttcc gcaaaccgtt tatgccgtta ctgccgggct tccgtcatgt
    12481 gccgtttggc aatatcgaag ccatgcgcac ggctcttaac gagtgcaaaa aaaccggtga
    12541 tgatgtggtt gcggtgatcc tcgaaccgat tcagggtgaa ggtggcgtaa ttctgccgcc
    12601 gccgggctat ctcaccgccg tacgtaagct atgcgatgag ttcggcgcac tgatgatcct
    12661 cgatgaagta caaacgggca tggggcgcac gggcaagatg ttcgcctgcg agcatgagaa
    12721 cgtacagccg gatatcctct gccttgccaa agcgctcggc ggcggcgtga tgccgattgg
    12781 cgcgaccatc gccactgaag aggtgttttc agttctgttc gacaacccat tcctgcatac
    12841 caccaccttt ggcggcaacc cgctggcctg tgcggcggcg ctggcgacca tcaatgtgtt
    12901 gctggagcag aacttaccgg ctcaggctga gcaaaaaggc gatatgttgc tggacggttt
    12961 ccgtcaactg gcgcgggaat atcccgatct ggtacaggaa gcgcgtggta aagggatgtt
    13021 gatggcaatt gagtttgttg ataacgaaat cggctataac tttgccagcg agatgttccg
    13081 ccagcgcgta ctggtggccg gaacgctcaa taacgccaaa acgatccgca ttgaaccgcc
    13141 actgacactg accattgaac agtgtgaact ggtgatcaaa gcggcgcgta aggcgctggc
    13201 ggccatgcga gtaagtgtcg aagaagcgta atatcaaatc ggatggcgat acgacgtcgc
    13261 catccgatct ttttatacga cgcgtacgcc cgcaggcatc atccgctccg gcgttaacaa
    13321 cacgctttca ctgccgtcat cggtttccgc acacaacagc atgcattccg acgtttcacc
    13381 acgcattttc gctttttgca gattgcataa gaccaccacc gttttcccca tcagctcttc
    13441 ttcgctgtag tacggcacca ggctggtcac ggtttgcagt gttttttccc ccacatcaac
    13501 ctgtacgatg tacagcttgt cggcgttttc atggcgtttc acttccacaa tctttccgac
    13561 gcgcatttcc agacgtgcaa aatcagcgta agccacggtt tccattgttt ccttcctccg
    13621 ggtaaaattt tactaaacta tagcaaaggt ttttctcaat cctgtaggct aaaaatggag
    13681 aatgcaggcg tgatcacatt cgtgagccgc tgtgttaccg ttacagcgtc aaagaaacgc
    13741 gctttattta ctgaaaacag gtgacccgat aagtacttcc tctacaatgg gggcgcacat
    13801 cagggaaagt aaaaaaggta aacatggcaa cactaaaaga catcgcaatc gaagctggcg
    13861 tatccctggc gacagtatcc agggtcttaa atgacgatcc gacattgaat gtgaaagaag
    13921 agacgaaaca tcgcattctc gagatcgccg aaaagctgga gtacaagacc agtagtgccc
    13981 gtaaactcca gacaggtgca gtcaaccaac accatattct ggctatctac agctaccaac
    14041 aggagctgga gatcaacgat ccttactatc tggcgatccg ccacggcatt gaaactcagt
    14101 gcgaaaagct gggcattgag ctcaccaact gttatgaaca cagcggatta ccagacatta
    14161 aaaacgtcac cggtatttta attgtcggca aacccacgcc cgccctgcgc gccgccgcct
    14221 gtgcgttgac cgacaatatc tgttttatcg actttcacga acccggcagc ggttacgatg
    14281 cggtggatat cgatctggca cgcatcagta aagaaatcat cgacttctat atcaatcagg
    14341 gcgttaatcg tattggtttt attggcggtg aagatgagcc aggcaaagcg gatattcgtg
    14401 aggtcgcctt tgcggaatat ggccgactga aacaagtggt acgcgaagag gatatctggc
    14461 gcggaggctt ttccagttcg tcgggttatg aactggcaaa acagatgctg gcgcaggaag
    14521 actatccgaa ggcactgttt gttgcttccg attccattgc tatcggcgta ctgcgggcaa
    14581 ttcatgaacg aggcctgaac atcccacagg atatttcgct tatcagcgtt aacgatatcc
    14641 ccaccgcgcg atttaccttt ccgccgctct ccaccgtgcg catccattcc gagatgatgg
    14701 gaagtcaggg cgttaaccta gtgtatgaaa aagcccgcga tggtcgcgcg ctgccgctgt
    14761 tagtcttcgt tcccagcaaa ttaaaactgc gcggcacgac ccgttaaatc cccttacaca
    14821 ctgtccggca atcgtttttg ccggacagtg ctgccgttta ttttcgtgat ccagttaaag
    14881 taaatgcatt tatctgctac tttttagtga aaattttact aagctcccca gcaattacac
    14941 aaactaccat caccatgaat ggttccgatt tctctctacc gggaggccct atgaatcgct
    15001 gggaaaacat tcagctcacc cacgaaaacc gacttgcgcc gcgtgcgtac tttttttcat
    15061 atgattctgt tgcgcaagcg cgtacctttg cccgcgaaac cagcagcctg tttctgccct
    15121 taagcggtca gtggaatttc cacttttttg accatccgct gcaagtacca gaagccttca
    15181 cctctgagtt aatggctgac tgggggcata ttaccgtccc cgccatgtgg caaatggaag
    15241 gtcacggcaa actgcaatat accgacgaag gttttccgtt ccccatcgat gtgccgtttg
    15301 tccccagcga taacccaacc ggtgcctatc aacgtatttt caccctcagc gacggctggc
    15361 agggtaaaca gacgctgatt aaatttgacg gcgtcgaaac ctattttgaa gtctatgtta
    15421 acggtcagta tgtgggtttc agcaagggca gtcgcctgac cgcagagttt gacatcagcg
    15481 cgatggttaa aaccggcgac aacctgttgt gtgtgcgcgt gatgcagtgg gcggactcta
    15541 cctacgtgga agaccaggat atgtggtggt cagcggggat cttccgcgat gtttatctgg
    15601 tcggaaaaca cctaacgcat attaacgatt tcactgtgcg taccgacttt gacgaagcct
    15661 attgcgatgc cacgctttcc tgcgaagtgg tgctggaaaa tctcgccgcc tcccctgtcg
    15721 tcacgacgct ggaatatacc ctgtttgatg gcgaacgcgt ggtgcacagc agcgccattg
    15781 atcatttggc tattgaaaaa ctgaccagcg ccagctttgc ttttactgtc gaacagccgc
    15841 agcaatggtc agcagaatcc ccttatcttt accatctggt catgacgctg aaagacgcca
    15901 acggcaacgt tctggaagtg gtgccacaac gcgttggctt ccgtgatatc aaagtgcgcg
    15961 acggtctgtt ctggatcaat aaccgttatg tgatgctgca cggcgtcaac cgtcacgaca
    16021 acgatcatcg caaaggccgc gccgttggaa tggatcgcgt cgagaaagat ctccagttga
    16081 tgaagcagca caatatcaac tccgtgcgta ccgctcacta cccgaacgat ccgcgttttt
    16141 acgaactgtg tgatatctac ggcctgtttg tgatggcgga aaccgacgtc gaatcgcacg
    16201 gctttgctaa tgtcggcgat atcagccgta ttaccgacga tccgcagtgg gaaaaagtct
    16261 acgtcgagcg cattgttcgc catatccacg cgcagaaaaa ccatccgtcg atcatcatct
    16321 ggtcgctggg caatgaatcc ggctatggct gtaacatccg cgcgatgtac catgcggcga
    16381 aagcgctgga tgacacgcga ctggtgcatt acgaagaaga tcgcgatgct gaagtggtcg
    16441 atattatttc caccatgtac acccgcgtgc cgctgatgaa tgagtttggt gaataccagc
    16501 atccgaagcc gcgcatcatc tgtgaatatg ctcatgcgat ggggaacgga ccgggcgggc
    16561 tgacggagta ccagaacgtc ttctataagc acgattgcat tcagggtcat tatgtctggg
    16621 agtggtgcga ccacgggatc caggcacagg acgaccacgg caatgtctgg tataaattcg
    16681 gcggcgacta cggcgactat cccaacaact ataacttctg tcttgatggt ttgatctatt
    16741 ccgatcagac gccgggaccg ggcctgaaag agtacaaaca ggttatcgcg ccggtaaaaa
    16801 tccacgcgcg ggatctgact cgcggcgagt tgaaagtcga aaataaactg tggtttacca
    16861 cgcttgatga ctacaccctg cacgcagagg tgcgcgccga aggtgaaacg ctcgcgacgc
    16921 agcagattaa actgcgcgac gttgcgccga acagcgaagc ccccttgcag atcacgctgc
    16981 cgcagctgga cgcccgcgaa gcgttcctca acattacggt gaccaaagat tcccgcaccc
    17041 gctacagcga agccgggcat tctatcgcca cttatcagtt cccgctgaag gaaaacaccg
    17101 cgcagccagt gcctttcgca ccaaataatg cgcgtccgct gacgctggaa gacgatcgtt
    17161 tgagctgcac cgttcgcggc tacaacttcg cgatcacctt ctcaaaaatg agtggcaaac
    17221 cgacatcctg gcaggtaaat ggcgagtcgc tgctgacccg cgagccaaag atcaacttct
    17281 tcaagccgat gatcgacaac cacaagcagg agtacgaagg gttgtggcag ccgaatcatt
    17341 tgcagatcat gcaggaacat ctgcgcgact ttgccgtaga acagagcgat ggtgaagtgt
    17401 tgatcatcag ccgcacagtt attgcaccgc cggtgtttga cttcgggatg cgctgtacct
    17461 acatctggcg catcgctgcc gatggccagg ttaacgtggc gctttccggc gagcgttacg
    17521 gcgactatcc gcacatcatt ccgtgcatcg gtttcaccat ggggattaac ggcgaatacg
    17581 atcaggtggc gtattacggt cgtggaccgg gcgaaaacta cgccgacagc cagcaggcta
    17641 acatcatcga tatctggcgc agcaccgtcg atgccatgtt cgagaactat cccttcccgc
    17701 agaacaacgg caaccgtcag catgtccgct ggacggcact gactaaccgc cacggcaacg
    17761 gtctgctggt ggttccgcag cgcccaatta acttcagcgc ctggcactat acccaagaaa
    17821 acatccacgc tgcccagcac tgtaacgagc tgcagcgcag tgatgacatc accctgaacc
    17881 tcgaccacca gctacttggc ctcggctcca actcctgggg cagcgaggtg ctggactcct
    17941 ggcgcgtctg gttccgtgac ttcagctacg gctttacgtt gctgccggtt tctggcggag
    18001 aagctaccgc gcaaagtctg gcgtcgtatg agttcggcgc agggttcttt tccacgaatt
    18061 tgcacagcga gaataagcaa tgaggatcat cgataactta gaacagttcc gccagattta
    18121 cgcctctagc aagaagtggc aacgctgtgt tgaagcgatt gaaaatatcg acaacattca
    18181 gcctggcgtc gcccactcca tcggtgactc attgacttac cgcgtggaga cagactccgc
    18241 gaccgatgcg ctatttaccg ggcatcgacg ctattttgaa gtgcat
//
LOCUS       DAJTMK010000079        18130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTMK010000079 DAJTMK010000000
VERSION     DAJTMK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18130
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.080"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(324..680)
                     /locus_tag="OO135_002260"
     CDS             complement(324..680)
                     /locus_tag="OO135_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5398093.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(747..1041)
                     /gene="yebG"
                     /locus_tag="OO135_002261"
                     /pseudo
     CDS             complement(747..1041)
                     /gene="yebG"
                     /locus_tag="OO135_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            1174..2352
                     /gene="purT"
                     /locus_tag="OO135_002262"
     CDS             1174..2352
                     /gene="purT"
                     /locus_tag="OO135_002262"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5398094.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(2408..3049)
                     /gene="kdgA"
                     /locus_tag="OO135_002263"
                     /gene_synonym="eda"
     CDS             complement(2408..3049)
                     /gene="kdgA"
                     /locus_tag="OO135_002263"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5398095.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGLKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(3086..4897)
                     /gene="edd"
                     /locus_tag="OO135_002264"
     CDS             complement(3086..4897)
                     /gene="edd"
                     /locus_tag="OO135_002264"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5398096.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(5132..6607)
                     /gene="zwf"
                     /locus_tag="OO135_002265"
     CDS             complement(5132..6607)
                     /gene="zwf"
                     /locus_tag="OO135_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5398097.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            6945..7814
                     /gene="hexR"
                     /locus_tag="OO135_002266"
                     /gene_synonym="yebK"
     CDS             6945..7814
                     /gene="hexR"
                     /locus_tag="OO135_002266"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5398098.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            7942..9384
                     /gene="pyk"
                     /locus_tag="OO135_002267"
     CDS             7942..9384
                     /gene="pyk"
                     /locus_tag="OO135_002267"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5398099.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(9444..10286)
                     /locus_tag="OO135_002268"
     CDS             complement(9444..10286)
                     /locus_tag="OO135_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR5398100.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHETTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(10309..11134)
                     /locus_tag="OO135_002269"
                     /pseudo
     CDS             complement(10309..11134)
                     /locus_tag="OO135_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(11227..12024)
                     /locus_tag="OO135_002270"
     CDS             complement(11227..12024)
                     /locus_tag="OO135_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCR5398101.1"
                     /translation="MIIDAHTHIGDFVKISMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQDDNNERMLRLVRQHAKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSEKVQDYIRLAQKYDLPVVSHTSNDYESSPQLFYEMALKY
                     PDVNFVMCHMGLATDNQEAIELIAKLPNLYGDTVWVRADMVYQAIKICGSDKILFGTD
                     NPINGLDTYNDDDFYNKYFTELKSKLTQSEFEQLMFTNAKKNFKIKI"
     gene            complement(12046..13845)
                     /locus_tag="OO135_002271"
                     /pseudo
     CDS             complement(12046..13845)
                     /locus_tag="OO135_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(13863..14696)
                     /locus_tag="OO135_002272"
     CDS             complement(13863..14696)
                     /locus_tag="OO135_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5398102.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFHG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEADKIDGAGHLTIFIQIILPLAKPILVFIGLVSFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            complement(14699..15987)
                     /locus_tag="OO135_002273"
                     /pseudo
     CDS             complement(14699..15987)
                     /locus_tag="OO135_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            complement(15990..17221)
                     /locus_tag="OO135_002274"
                     /pseudo
     CDS             complement(15990..17221)
                     /locus_tag="OO135_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
     gene            17493..>18130
                     /locus_tag="OO135_002275"
     CDS             17493..>18130
                     /locus_tag="OO135_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5398103.1"
                     /translation="MVLIKKNVLSASIATVILGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGA"
BASE COUNT         5108 a         4253 c         3878 g         4891 t
ORIGIN      
        1 tgctcctcaa taaccatttt tagtgcctgt agcatagcgg atgaggatgt cagaaaatgt
       61 atatcgagac aaaagatgcg caaataagcg gggcggtagg ccgttattca aagaaattcg
      121 cgccaggtaa ggtatttact gtatatcagg tgataagggt attttaggtg aaagtagttt
      181 gtcgttttga gtggcattgt ttgatgctga ttgcccgatg cgacgctgtt gcgtcttctc
      241 aggcctacga ttgtggtgcg ttctggggcg gacaaggcac tcgcgccgca tccgccaacg
      301 gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt ccgccattcc cgctttgcag
      361 tccacgctga cctggtaatg aatatcggca cttttaccac gcacggttag cggtactgac
      421 catttatcat ctttaccctg aatgtcctgc aaactgaccc aagccacggg atcggcctga
      481 ccgacaattt tttgatcatc tgcccaacgc gccacgcgat tttgttgata atcacgtttt
      541 acgctcgcgg caattccggc agcatccaga ccttcacact ttgggaaagt gaccgacttg
      601 ctggtttcat tattggcagc gaaaactgat gcgcaggcag aaaccaacaa cagccctaaa
      661 aacgcccctc tttttttcat gtttttctcc atagcacaat gattcaggag aaagcatggt
      721 acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg aggcatcttc ctcttcggat
      781 tcagcaccga cgacctgtgg gtggggacgg taatttgccg gttttcagaa tggtgctcag
      841 cacatctttt tgttctgcca gccatagcga aagggcttca cgttgctcgt cttccatttg
      901 cactggagaa ttagtcagcc aggtgtcaag cagatccgcc gtatcaagca ttttgtcata
      961 agcatcggct tcctttttgc tggtaaacga cattttctct tcgccctcac gaatgactac
     1021 gtatttaact tcaaccgcca tttgcagcct ctcataataa ctgtgatttt atacagtata
     1081 tttcttttcg gttgagaatc aacatcagca ataaagacac acgcaaacgt tttcgtttat
     1141 actgcgcgcg gaattaatca ggggatattc gttatgacgt tattaggcac tgcgctgcgt
     1201 ccggcagcaa ctcgcgtgat gttattaggc tccggtgaac tgggtaaaga agtggcaatc
     1261 gagtgtcagc gtctcggcgt agaggtgatt gccgtcgatc gctatgccga cgcaccagcc
     1321 atgcatgtcg cgcatcgctc ccatgtcatt aatatgcttg atggtgatgc attacgccgt
     1381 gtggttgaac tggaaaaacc acattatatc gtgccggaga tcgaagctat tgccaccgat
     1441 atgctgatcc aacttgaaga ggaaggactg aatgttgtcc cctgcgctcg cgcaacgaaa
     1501 ttaacgatga atcgcgaggg tatccgtcgc ctggcggcag aagagctgca gctgcccact
     1561 tccacttatc gttttgccga tagcgaaagc cttttccgcg aggctgttgc tgacattggc
     1621 tatccctgca ttgtaaaacc ggtgatgagc tcttccggca aggggcagac gtttattcgt
     1681 tctgcagagc aacttgctca ggcatggaag tacgctcagc aaggcggtcg cgccggagcg
     1741 ggccgcgtaa ttgttgaagg cgtcgttaag tttgacttcg aaattacctt gctaaccgtc
     1801 agcgcggtgg atggcgtcca tttctgtgca ccggtaggtc atcgccagga agatggcgac
     1861 taccgtgaat cctggcaacc acagcaaatg agcccgcttg cccttgaacg tgcgcaggag
     1921 attgcccgta aagtggtgct ggcactgggc ggttatggtc tgtttggtgt cgagctgttt
     1981 gtctgtagtg atgaggtgat tttcagtgag gtctcccctc gtccacatga taccgggatg
     2041 gtgacgttaa tttctcaaga tctttcagag tttgccctgc atgtacgtgc cttcctcgga
     2101 cttccggttg gcgggatccg tcagtatggt cctgcagctt ctgccgttat tctgccacaa
     2161 ctgaccagtc agaatgtcac gtttgataat gtgcagaatg ccgtaggcgc agatttgcag
     2221 attcgtttat ttggtaagcc ggaaattgat ggcagccgtc gtctgggtgt tgcgctggcg
     2281 accgctgaga gtgttgttga cgccattgaa cgcgcgaagc acgccgccgg acaggtaaaa
     2341 gtacagggtt aaacccggca aaaaaacgct acaaaaatgc ccgatcgtag atcgggcgtt
     2401 ttgactttta cagcttagcg ccttctacag cttcacgcgc cagcttagta atgcggtcgt
     2461 aatcgcccgc ttccagcgca tctgccggaa ccagccagga accaccgatg cacagcacgc
     2521 ttttcagcgc caggtagtca cggtagttag ccggagaaat accacccgtc gggcagaaac
     2581 ggacctggga gaacggaccc gcgatcgcct gcagggcttt caagccgccg ttagcttcag
     2641 ccgggaagaa tttgaactct ttcaaaccgt agtccatacc cagcatcagt tcggaaacag
     2701 tgctgatccc cggaattaga ggaatagtcc cttcggtagc agctttcagc agcggctcgg
     2761 tcagacccgg gctaattgcg aactgtgcac ccgcttcagt gacttctgcc agctgctgtg
     2821 gattcagcac cgtaccggca cccacaatcg cttcaggcac ttctttggcg atagcacgga
     2881 tagcgtcaac tgcacactcg gtacgcagag tcacttccag aacgcgcacc ccaccagcaa
     2941 ccaacgcttt tgccatcggc actgcgtgtt ccagtttttt taccacgata accggtacaa
     3001 ccgggccggt ggtcaggatt gattctgcac ttgttttcca gtttttcatc agagttttct
     3061 ctcgcctgat tacaaatttg tcgtcttaaa aagtgataca ggttgcgccc tgttcggcac
     3121 cggacagttt ttcacgcaag gcgctgaata attcacgtcc tgtccccacg cgtgacgcgc
     3181 tcaggtcagg aatgtgcggt tcgcgagcag ccagttccgc ttcgtctacc agcagcgtca
     3241 gttcgcctgt ctgtccattc acacgaatga tgtccccgtc gcgcactttt gccagcagcc
     3301 cgccatcgta ggcttctggt gttacgtgga tagctgacgg cactttacct gaagcgccgg
     3361 agagtcgtcc atcggtaact aacgcaattt tgaaacaccg gtccaataat acaccaagtg
     3421 gcggcatgag tttatgtaat tctggcattc cgttcgcttt tggcccctga tgacggacaa
     3481 caacgacaca atcgcggtcc agcaaacccg cttcaaaggc cggcataacg tcatgctggc
     3541 tttcaaaaac aaccgctggc gcttcaatca cctggttctc aaccggcacg gcagaggttt
     3601 tcataaccgc acggcccagg ttaccgctta acacttttgt cccaccatga tgagagaaag
     3661 gttgttcgaa ggaagcgatc acattgctgt cgagtgattt ttccgcccct tcccgccagt
     3721 ccagttcacc attattcagc catggttcaa gggtataacg agacagacca aagcctgcca
     3781 ccgtattgac atcttcatgc agcaggcctg ctttgagcag ttcacgcacc agaaccggta
     3841 cgccacctgc cgcctggaag tggttaatat cggccggacc gttcgggtag agacgtgcca
     3901 tcagcggtac aacatcagaa aggtcagaga agtcatccca gttaatctga ataccggccg
     3961 cgcgcgccat cgccaccagg tgcatggtgt ggttagtgga accaccggtc gccagcagtg
     4021 caacgatacc gttcaccacc actttctcat cgatcatctt accgatcggc atccattcat
     4081 taccattacc ggtcatgcgt gtaacctgac gcgcagcggc agcggtcaaa gcatcgcgca
     4141 gcggagaatc cggatgaaca aaagaagagc ctggcgactg catccccata aattccacca
     4201 ccatctggtt ggtgttggca gtaccgtaga aagtacatgt tcccggcgca tggtaagacg
     4261 cggcttctga ctccagtaag gccatgcggt ccactttacc ttcggcataa agctggcgaa
     4321 tacgcacttt ttctttattt ggcaaaccgc ttgccatcgg tccagacggc acaaacaccg
     4381 caggcaaatg accaaacgac agggctgcca tcgtcagacc cgggacaatc ttgtcgcaca
     4441 caccgaggaa cagagcacca tcaaacatgt tatgggacag ccccaccgcc gcagacatcg
     4501 ctatcacttc gcggcttagc agcgacaatt ccattccatc ctgcccctgg gtgacaccat
     4561 cacacatcgc cggaacaccg cccgcaacct gaccaaccgc attcgcttca tgcagggctt
     4621 tacgaatgat ttctggatag tgttcataag gctggtgcgc ggagagcatg tcgttatagg
     4681 aggtgatgat ggcgatattg ttacgcaaca tgcttttcaa agaggctttg tcttctggct
     4741 ggcaggcagc gaaaccgtgt gccaggttac cgcatgccaa ctgcgaacga tgaacggtcg
     4801 acgttttcgc ttgttctatc cgggcgagat aagcagagcg agtctcgcgc gaacgttcaa
     4861 tgattcgatt tgttacgcgt aacaattgtg gattcataaa ggctcctgaa attgagttgt
     4921 cagagcagga tgattcacaa cgcgtttcat tcagaggatt tatgagtgaa acgcctgtaa
     4981 ccggagctca tagggcaaaa acgtttcagt cagtgtaata aaaaaagcct cgtgggtgaa
     5041 tccgcacgag gcctgaaagt gtaaaaattg ttctacaatc tgtgcaagat catgttaccg
     5101 gtaaaatgac cataaaggat aagcgcagat attactcaaa ctcattccag gaacgaccat
     5161 cacgggtaat catcgccacc gaggcaacgg gtccccaggt tccggcctga tacggtttcg
     5221 gcgcatcatt gtccatcgcc cacgcctcag taatggagtc tacccatttc caggcttctt
     5281 ccacttcatc acgacgtaca aacagtgcct gaataccacg catggtttcc agcagcagac
     5341 gttcataggc atccgccaga tgcgtctgat taaaggtttc tgaatagctc agatccagct
     5401 tggtgatttg caggttatgt ttgtggtcaa ggccaggaac tttattcagt acctggatat
     5461 ccacgccttc atcaggttgc agacggatag tcagtttatt ctgcggcaga tcctgccacg
     5521 actctttaaa cagattcagt tcaggtgttt tgaaatagac cacgacttca gaacatttgg
     5581 tcggcagacg tttaccagta cgcaggtaga atggcacacc ggcccagcgc cagttatcaa
     5641 tgtcgacgcg gatcgccacg aaggtttctg tattgctgct cttgttcgcg ccctcttctt
     5701 ccagatatcc cggcactttt ttgccctggg cgaagcccgc agtatattgc ccacgtacgg
     5761 ttttttcgcg tacgttggag cggtcgatgc ggcgcaggga cttcagtact ttcacttttt
     5821 catcgcggat gctgtctgcg ctcaggtcag atggcggaga catcgcaatc atgcagagaa
     5881 tttgtagcag gtggttctga atcatgtcac gcatctgacc ggctttatca aaatagcccc
     5941 agcgcccttc gatccccact tcttctgcca cggtaatctc aacatgatca atggtgcgat
     6001 tgtcccagtt attcacaaac agggagttag caaaacgcag cgccaacagg ttcagcaccg
     6061 tttctttacc aagatagtgg tcgatacggt aaacctggca ctcctcgaag tattcgccaa
     6121 cctgatcatt gatttcctgc gaggtcgcca gcgacgtccc cagcggtttc tccatgacta
     6181 cgcgtgccgg tttagcattc agttttgcct cgccaagccc tttgcaaatt gcgccaaaag
     6241 tgctgggcgg catggcaaag tagttaatgg tgatacgatt tttttgatcc agcatcgcgc
     6301 cgagacggtt gaatgcagca gtgtcattga catcgagatt acaaaaatcc agacgtgcac
     6361 tcagggtgtc ccataaacct tcatcaatgg tttctttcat gaaagtttcg agcgcctcgc
     6421 ggacaacttt ggtatatgcc gctttatccc agtcagcacg ccctacgccg ataatccggg
     6481 tgtccgggtt gagctgaccg gctttttcca gttgatacag ggaaggcagc aatttacgac
     6541 gcgcaaggtc gcctttcgcg ccgaaaatga ccaagtcaca ggcctgggct gtttgcgtta
     6601 ccgccatgtc attctcctta agttaactaa cccggtactt aagccagggt atacttgtaa
     6661 ttttcttacg gtgcactgta ctgcttttac gagcttgcga aaactgtaaa cgcttatcca
     6721 cccgtgcgat tacgggaaaa gcgcgcaaag tgcggcaaaa ctgataaaaa aatcatcgtt
     6781 ttccattcgg taaaaatctg acactgatca tgttatgaaa aaaaataaca acttttttat
     6841 ctgcttttgt cattaacacg gcacacaggc gtaatatctg acaaaactgc atttcgattt
     6901 ctttcagtgc ggaaatcgtc attacccgtg agtctcttta catcatgaat atgctggaaa
     6961 aaatccagtc tcagctggaa catttgagca aatcagagcg caaagttgcc gaggtcattc
     7021 tggcttcgcc cgataacgcg atccattcga gtattgctgc tatggcactg gaagccaatg
     7081 ttagcgaacc gacggtgaat cgtttctgtc gcagcatgga cacgcgcggt tttcctgatt
     7141 ttaaacttca tctggcacag agtctggcga atggcactcc ctatgttaat cgcaatgtca
     7201 atgaagatga cagcgttgaa tcatacacag ggaaaatatt tgagtccgca atggcaacgc
     7261 ttgatcatgt ccgtcattca ctggataaat ctgccatcaa ccgcgccgtc gacttgctca
     7321 ctcaggcaaa aaaaatcgcc tttttcggat taggctcttc agccgccgtt gcccacgatg
     7381 cgatgaataa gttctttcgt tttaatgttc cggtggtgta ctccgatgat atcgtgctgc
     7441 aacgcatgag ttgtatgaat tgtagcgacg gagacgtggt ggtgctgatt tctcacactg
     7501 gaagaacaaa aaatctggtc gagctggcgc agctggctcg cgaaaacgac gcaatggtga
     7561 ttgccctcac ctctgcgggt accccgctcg cccgggaagc aacgctggca attaccctcg
     7621 acgtaccgga agatactgac atttatatgc ccatggtttc tcgacttgca cagctgaccg
     7681 tgatagatgt gctggcgaca ggatttactt tgcgacgcgg tgcaaaattc agagataact
     7741 tgaagcgggt caaggaagcg ctgaaggaat cgcgttttga taagcagtta cttaatttaa
     7801 gtgacgatcg ctaaaaacga ctgtcactgt cctaatctta tacgacatcc gaatgagatt
     7861 aatttatcgc catcgcggcg ttatttcatt cggatttcat gttcaagcaa cacctggttg
     7921 tttcagtcaa cggagtatta catgtccaga aggcttcgca gaacaaaaat cgttaccacg
     7981 ttaggcccag caacagatcg cgataataat cttgaaaaag ttatcgcggc gggtgccaac
     8041 gttgtacgta tgaacttttc tcatggctcg cctgaagatc acaaaatgcg cgcggataaa
     8101 gttcgtgaga ttgccgcaaa actggggcgt catgtggcta ttctgggtga cctccaggga
     8161 cccaaaatcc gtgtatccac ctttaaagaa ggcaaagttt tcctcaatat tggggataaa
     8221 ttcctgctcg acgccaacct gggtaaaggt gaaggcgaca aagaaaaagt cggtatcgac
     8281 tacaaaggcc tgcctgctga cgtcgtgcct ggtgacatcc tgctgctgga cgatggtcgc
     8341 gtccagttaa aagtactgga agttcagggc atgaaagtgt tcaccgaagt caccgtcggt
     8401 ggtcccctct ccaacaataa aggtatcaac aaacttggcg gcggtttgtc ggctgaagcg
     8461 ctgaccgaaa aagacaaagc agacattaag actgcggcgt tgattggcgt agattacctg
     8521 gctgtctcct tcccacgctg tggcgaagat ctgaactatg cccgtcgcct ggcacgcgat
     8581 gcaggatgtg atgcgaaaat tgttgccaag gttgaacgtg cggaagccgt ttgcagccag
     8641 gatgcaatgg atgacatcat cctcgcctct gacgtggtaa tggttgcacg tggcgacctc
     8701 ggtgtggaaa ttggcgaccc ggaactggtc ggcattcaga aagcgttgat ccgtcgtgcg
     8761 cgtcagctaa accgagcggt aatcacggcg acccagatga tggagtcaat gattactaac
     8821 ccgatgccga cgcgtgcaga agtcatggac gtagcaaacg ccgttctgga tggtactgac
     8881 gctgtgatgc tgtctgcaga aactgccgca gggcagtatc cgtcagaaac tgttgcagcc
     8941 atggcgcgcg tttgcctggg tgcggaaaaa atcccgagca tcaacgtttc taaacaccgt
     9001 ctggacgttc agttcgacaa tgtggaagaa gctattgcca tgtcagcaat gtacgcagct
     9061 aaccacctga aaggcgttac ggcgattatt accatgaccg aatcgggtcg taccgcgctg
     9121 atgacctccc gtatcagctc tggtctacca attttcgcca tgtcgcgcca tgaacgtacg
     9181 ctgaacctga ctgctctcta tcgtggcgtt acgccggtgc actttgatag cgctaatgac
     9241 ggcgtagcag ctgccagcga agcggttaat ctgctgcgcg ataaaggtta cttgatgtct
     9301 ggtgacctgg tgattgtcac ccagggcgac gtgatgagta ccgtgggttc tactaatacc
     9361 acgcgtattt taacggtaga gtaatctcac ctgccccggc cgctgatggc cggggtattt
     9421 agcaacacat aaattaggac tacttattca atccaatcat aatcacagcc ccctgtaaaa
     9481 tgaatacagg gatagcctga atggagatct aaatagcttg caagacgtcg catacccgct
     9541 tcctctgcaa cactatgtcc aataactgcc atcggtaagc cactatcttt ggagtattgt
     9601 gtctgcaccc atgttttaat tgaatcgtta atcgtaatat aataatcagc ctgatattcc
     9661 atatattgta ttggatcaca aaaacagcct gccccaatgc caacagttct gactactctg
     9721 gctttatctc cataaaattg aatgccagga agattatata ttttcaaatt tttggcaaaa
     9781 cgtttggtaa tgttaatggc actgtcaggt tctatagcat atacacagta catctcatca
     9841 gaagcaacaa tatttgtaac atccagttct aactgctgag ccaaagcctt agacattccg
     9901 aacgtaggct ctcgatccat gacatcatgg caacgaatga ttaccaaatt atttttatgc
     9961 aaaaactcct ttttttgctc aattattttt aaataactga gttcaccgcg agtataatcc
    10021 attatttttc tggcccatga aaactcaaga tcgtcaccct ccagatcagc ataagaataa
    10081 aatgtagtct catgtgtaat aatgatattg acaccttcaa aaacagcctt tcttaaaaca
    10141 tccattgagg caatccagca ggtcccaatt ttattaataa gtgtcgcctc agcaccaaaa
    10201 ataattttat caacagttgg ttgcgaaaca gcaaccattg aatttaaaaa acaattaaga
    10261 tccgcggctg ttttaatatg atgcattcaa catccttcac aggtttcatc agcaataaaa
    10321 taatttcaac atttctggga aacgttttgc ccatgcagac tcagagtggg tatcacctcc
    10381 ccataaatga taatctatag ttacattgcg ttgtttacgg agactcacgc ataatttttc
    10441 agcaccggaa atatagactt caggaaagtc ttcgcgcata ccactggttt cctgcgtacc
    10501 gacatcgaga tatacagtaa gatcttcaac gatgttgttt tcctgaataa aattcagcat
    10561 tgcgctgtca ttgaaccata gtgccggcga aaaaacgcca acttttgaaa aaacgtcctg
    10621 acgaagaatt acggcaaaca aactgatgta cgcacccata gaactacctg ctatcgctgt
    10681 attattacga tctgggagag ttctacaatt actgtcaaca tagttcttga gcgtattcac
    10741 aataaacgcc ttttcctcca ctataatcca cagcagggtc ccagccaggt aagggaaaag
    10801 atgtgtttcg cggccatgga gaatactcat ccatacggct gaatttacca tgagacgatg
    10861 cgttatcaat ccccacaatg ataacaccgt cgtattgtga atttgggaaa aaagcatcta
    10921 ccgcctcagg tatatcccat acatgtggac caaaagatac agatttatca aataaatttt
    10981 gcccatcgtg catatatatt acagggtaac tgatatcaga ttcataatag aacgcaggca
    11041 gtaatattct tatttcgcga ctactcgcca attgtggcat aggaaaagat tgcaacacat
    11101 gatagcgatt cccatagttc tcaatatcgt tcatgattta aattaacctc aatgcaaaat
    11161 gaaatataac atctaagcac tcccctacca cattctatat ggggagcact tagaaatgac
    11221 taataactat atttttatct taaaattttt tttcgcattg gtaaacataa gttgttcaaa
    11281 ctcactttgc gttaatttac tttttaattc agtaaaatac ttattataaa aatcatcatc
    11341 attgtacgta tccagcccat taataggatt atcggtaccg aataaaatct tatcactacc
    11401 acagattttt atcgcctggt aaaccatatc cgccctaacc cacacagtat cgccatagag
    11461 attaggaagt tttgcaatca attcgattgc ttcctgatta tctgtcgcga gccccatatg
    11521 acacataaca aaattcacgt ctggatactt aagtgccatc tcataaaata actgtgggct
    11581 ggattcataa tcattagaag tatggctaac taccgggaga tcatattttt gagcaaggcg
    11641 tatataatcc tgaacttttt ctgagttgaa agccattttt gaatgataag ggtgaacttt
    11701 gatgccataa atgatatctc gattgctggc aatcatatct tcaaagtctt gatcacaact
    11761 ttccagacga ggcttaatcc acataaacgc gccaattcgc tttgcatgtt gacgaacaag
    11821 tctgagcata cgttcattgt tatcatcctg tgtaacttga tcttcatccg gaatcgggtt
    11881 ctggtcatgg tcaacttcaa cagcagatcc acaggaacat aacgcaaaat caatattata
    11941 cttgtccaga gatgccagaa aaacatcttc tggcatgctg attttcacaa aatcaccaat
    12001 atgagtgtgt gcatcaatta tcataacaaa catcctaatt gaaatttaaa aatcataaag
    12061 atcagcagca ggtaaacctt caatttcata actgaaaccg gagatctcca tacttcttcc
    12121 gtcataaata aagttggcag tatcttcact attatttaag aagaaagtga tgttatcgcg
    12181 ttgataagcg atcaccgtag gatgttcaac atctttccat tgtattaatg cctcacacca
    12241 ttgtggatgt tttttacgta gacgtattaa ccaacgtata aattgccgta attcaagatc
    12301 ttgtttgttt tcatcccata tcatacattt tctattattc tcaagcgtca ttgatttaaa
    12361 accatcaatt cctatttcgc taccgtagta gatacaagga gagcccgctt gagttgacat
    12421 aaataaatag gcgagtttaa cttttcgttt atcattcaaa caaacgctta aaatacgcgc
    12481 ggtgtcatga ctatccagaa gattaaacat aacttcagtt atatttttag gataacaaag
    12541 ataagaacgt gtaacggatg tcataaattc ttgttttgtt gtatcctgta acgcaaaata
    12601 atcaataatc ccatatgtta agggataatt cattaatgaa tcaaactgat caccacgcaa
    12661 ctagggggtg ccctcatgcc atatctcact caaaatataa cattcaggtt tgatatcttt
    12721 aatcaccttt ctgaaatctc gccagaaatg atgatccact tcattcgcaa catccagacg
    12781 ccaaccatca atatcaaaat tttgcgtcca atagcgaact atactaagta agtattcacg
    12841 gcactcttca ttctcagtat ttagcttagg catttcaata acattgccaa aggtttcata
    12901 gttaaagttt ttaaaatccc attcactttt tggagtatcg ggataaacag gcaatttatg
    12961 aatccagaac caatcggcgt aacgtgaatt ggcaccattg cgtacaacgt caagccatat
    13021 tggagagtca gcgcctaaat gattgaatac tgcatccaac atgattttta ttccacgctt
    13081 atgcgcttct tcaatcaaag tatggaaagc aatattccca cctaaatgtg gatcaacact
    13141 aaaatgatcg atggtgtcat atttatggtt ggatgctgag gtaaaaatag gacaaaaata
    13201 aatcccatta atacccagat cttccagata atcgagttta tcaataaccc cccacagatc
    13261 cccacccata aagttgaatg aagtcggtgt actgccccac ggttctaccc cttctgggga
    13321 tatttcaggt ctgccgttac agaaacggtc tggaaaaatt tggtaccata ccgtattttt
    13381 tacccaggag ggggtattta gaacgtcaat tttattaaga taagggaagc aaaaaaagtt
    13441 atttaagcgg cttaactctc gttcttcgca ctccggtgaa ctgatatcaa cacacctttt
    13501 ctcaccaaaa agaaacttct ctccctcttt cccaaaaagt ataaaaccgt aacggcaacg
    13561 gcgtttttga ggtttgaaca ctgcaaacca gtgatcatga aattcggtga cagcttcttg
    13621 ttccatctcg atctcgttgc cgccaatcca gccgaacgct tctgtcccgg ccatgttacc
    13681 gccgtcaaga ccaccttcag cccaattata tggatcgcct atccagagaa taactttatc
    13741 aacttctcct cttaatgtgc gtaaccgtac atgcaaagtt tcgttatcat aggcatagct
    13801 atcttcactt tttgctgagt gttttaacga actaactgta atcataaata ctaatcccta
    13861 aattattcct taacggcacc gctaacaagt ccagtagtta tatgcttctg tgtgaataca
    13921 aataacaatg tgataggtac tgcaactaaa agcgccccag cagcaaaaag cgtataattc
    13981 tctgctgaat tagaagcaat ccaactaaaa atgccaatcg ccagggtcat tttatcctca
    14041 ctacgaagca ccaacgttgg caaaatgaag tccatccatg gtccagtgaa cgaaactaat
    14101 ccaatgaaaa cgagaatcgg cttagccaat ggcaatatta tctgaataaa aatagtcaga
    14161 tgccctgctc catctatttt atcggcctca tccaaagaag ttggaatagc atcaaaataa
    14221 ccttttacca accacgtcat gaagggcaat gaaccggtta cataaaccag aagcaaccca
    14281 gtatatgtgt ctatcaaatt ggcctttgat agcaggatat aaatggcagt cattgataaa
    14341 aatgcaggaa acatttgcaa tacaagaata ctcattaacg tattacgctt tccatgaaat
    14401 cgataccttg aaaaaacgta ggcggtcatc gtcacaacga acagtgaaat taacatgtta
    14461 gcggtagcaa gaatgaaagt gttcaaatac cactgcatat atggcgtatc cgtgaacaac
    14521 gttatgtagt ggtcaaatgt aaactcgatg ccactaaatg ttgaactaaa tagattattc
    14581 ccttttttaa aagatgccat tatcgtccat atcactggcc caagcacaag aatagagttg
    14641 cagattaaaa aaagataaac taagagcgtt gatattttac gtaaaaattt atccattgtc
    14701 acatccctac atcatcttta aatgatttca tccgtctgaa ttgccatatt gctataaccg
    14761 ataagaagat aaatattatg atggagatta cagaagctat atgatattgc tggaaattca
    14821 atgtgagttt ataaatccag gtaataaaaa tatcggtatg cccagcgaat ctgtattccg
    14881 gattagtcgg accaccttca gtcagaagat aaattgcacc aaaattattg aaattatatg
    14941 caaatgtcat taccagagtt gacgcaacct gatgtaacac cattggcaat gtaatttcct
    15001 taaactgttg ccatttagtt gcgccatcga cctcactagc ctcatacaaa tctcgggata
    15061 tgtttgttaa tgcacccgcg atcaatagca taaaatacgg tgcccctacc caaacacaaa
    15121 cggcaattac tgttattttt gcatttaatg gaaccgacag aaaatcaact gaattcattc
    15181 cgatactgtt cagaaacgag ttaaccggac caattccatt taaaagaaga cgcaaaataa
    15241 gtaacgttac aaatgtgggt atggcatatg gcagtataaa tatcaatcgc cagacttttg
    15301 cgaaacgtat atttttttag ccagtgctaa agccagaata aagccaaaac tgaaggtaaa
    15361 aaccgtcgcg caaattgccc aaataaatgt ccagagagcg acacctagaa acgtatcaga
    15421 ccatatttta aatttaaaca acattatgaa atttttaatc ccaacccaat caaccaggtt
    15481 ctttggtggg atgtgatttg gcgcagcata gtttgtaaac gatacaagaa ctgtaattac
    15541 aataggtaaa acaatgaatg ctatacttgc taaaaatgcg ggcgttagga ctataaatgc
    15601 aaatttatcc tcatagagct tatataactg ttgtttaaaa gtcagatggc aataagatga
    15661 gttccgcgca tctttaatat taaggatgta aatagtggcg aataagccaa caataatggt
    15721 cgctatcacg ccctctacaa gcataaatat agaattgtca ccctgaataa cagtcaaccc
    15781 ttttcgaatt tgcgcgacat cacctaatgt gaacaaacct attaatgtat gcacaatatc
    15841 ggaaagataa agcaaaaagc atacttgtat tgcaaagaat atcccgcctt tcacataata
    15901 atgacgtgat aactgagtcc cgcccataat caaacaggaa ataggtagca caggtgcact
    15961 cttcattttc agttgttgtg tattcacaat tactcctgaa gcgaaagttg ttcttcaata
    16021 attgtttgtg catgatttaa aacttcatcc ggtgttttat tctcaaccca ggtagccaaa
    16081 atagcattag tcattggaga ccatagataa gacatttccg gtattgatgg cattgcgtca
    16141 gaataattag cttgagcaat aattgctttt atagcttcac tacctgtctg gctaattttg
    16201 ttcataagtg aatcgattgg tgggatgaga ttcgtcattt cataacgctt catcaacatc
    16261 ttatccgttg tcaggtagtc agcgaataat tcagcggcat gaggatatgc ggtaaatgag
    16321 cttacaacgg ccagccgtac tgttgaaaat gtgcgaggct gatgaccatc taatgttggg
    16381 atgcgtgtca cgccaaaatt gattccgctg tcaatgtaac cttgtatgga ccaaggtccg
    16441 tcgattacag cagcaacttt accttcatta aacagacctc ttagaacttg tggattaccc
    16501 atatcaagag gattagaggg attcgctttc tttaagcgtt tcatcgtctc tagccctttc
    16561 ttcgcttcag atgaagcaat gcctagagct ttaggatcgt taccggtttt accgaattcg
    16621 ttggcaccat acaaggtaat aaacatacgt gaaacataat aattttgtac atcccatagc
    16681 agagcatatt tatgctcgga tgaattatta aacttttact gaaagtattg atttcttcga
    16741 atgtttttgg tgcggtgttt aacaaatcct tattatagaa aagcgccagc gttgcgaaac
    16801 ttacaggaaa accatagctg atgttattat atgtagctgc cgatactgcg cctggaataa
    16861 atgttttttt aatccgctca gctgagacca tgttttccat aaccccgcca gcaacgacta
    16921 accgccccag ggtatcatgt tcaatttcag ctacatcagc tacacgagtc gtacccccat
    16981 cctgcataat tcttgatgct gcatccattg gcgctatatt gcgaaatgta aactttacct
    17041 tgtagccaaa atctttattg aattcctttg ctgcatattt catatattcc acgcttgtgg
    17101 catcagacca aactaacaac tcagcatcag tttcaggaat taatttttct gccatcgcct
    17161 ggggtgttag cgtactggaa tttgcaagaa ttataactgt gacgatattt gaaagcttca
    17221 ttatatttac ctcatgaata acatctttaa gtagtatatg atgtacgact acataagcat
    17281 tcacgccatt atcaacacaa atcaaggcca atcaactgtc aaataagatt ttaatgatga
    17341 ataactgcaa atagtgatgt atatcccatt tgcagtaatt tgttgagaaa aaaatatttt
    17401 attgccgcag agtgtttagg agtacatgag gtgcgactat atggaataac agaaatcata
    17461 tcttaaattg atattttgga aggaactcat ttatggtttt aataaaaaag aatgtcttat
    17521 cggcttcaat tgcaacagtc attttaggct caacaggagc ctttgcggca aacgatcgcg
    17581 gctttacctc cgacactgac tggtcagaag aacaagcggg ctttaatgga catgttggta
    17641 ctacatttga atatgagacc aaaagcactg ataactattt aggtaaaaca gtaaaggaat
    17701 atactaaaac acacgagatt ttccaggcct atttcagcca acctaattgg caatttgcag
    17761 caatctatgg ccttaagtct atcgatcgcc gacaagaaga aaaaggctat cacgaaaatg
    17821 aaacaagtct gcaaaactat atatcactaa acaaaacgtt cactattggt aacggtttcg
    17881 acttcagtct ggtatacgac ctgatgtata ccgacggtaa tatagatagc ccttatgtca
    17941 caggcttgca tactgtaact gttgacaaca gttttcgccc tacgttgacg tactttaact
    18001 ctgactggaa cgcaggtttt tattcaaacg ttgaatactt gtacagtcga aatgataaga
    18061 gctcctgggg cgtacgcgaa gaagaaggtc aaagtatact atttcagccc tataaacgtt
    18121 ttggtgcatg
//
LOCUS       DAJTMK010000080        18047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTMK010000080 DAJTMK010000000
VERSION     DAJTMK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18047
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.081"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            35..352
                     /locus_tag="OO135_002276"
     CDS             35..352
                     /locus_tag="OO135_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HCR5398104.1"
                     /translation="MATDSQLTIETALNVGLALLGYFYIVFCSRRWLSLLFLKKWNKR
                     RKQDERQKAMNAFSEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTSRKNH
                     ISP"
     gene            306..620
                     /locus_tag="OO135_002277"
     CDS             306..620
                     /locus_tag="OO135_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398105.1"
                     /translation="MTITKQRVEKIIYRHEMGLNSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMDGCDSCGQDCGADMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAASE
                     QD"
     gene            607..1152
                     /locus_tag="OO135_002278"
     CDS             607..1152
                     /locus_tag="OO135_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5398106.1"
                     /translation="MSKIDYQALREAAEKATKGKWAVEFDDEIYSTDGINHEQIAMVF
                     SENESRDAEFIAAANPATVLALLDERERNQQYIKRRDQENEDIALTVGKLRVELEAAK
                     SKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYGHPINADERQVMIPKENGS
                     WLYLIDLEHALRVADIRIKGE"
     gene            1154..1417
                     /locus_tag="OO135_002279"
     CDS             1154..1417
                     /locus_tag="OO135_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398107.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPLPKKIGFYTIGDKTIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            1428..1940
                     /locus_tag="OO135_002280"
     CDS             1428..1940
                     /locus_tag="OO135_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5398108.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLA
                     AEPVAKIIAHYPLGVDVGKQKFVQAIRELPDFGGYLFAAPPAPIVPEEMYRQDAPVEG
                     SSKAAAYATGWNDCREAMLQSGNFRENKDSSTNNFRKIPEASTSSPVTPDGWISCSER
                     MPDDRQEVNQ"
     gene            2025..2267
                     /locus_tag="OO135_002281"
     CDS             2025..2267
                     /locus_tag="OO135_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5398109.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            2424..2678
                     /gene="xisR"
                     /locus_tag="OO135_002282"
     CDS             2424..2678
                     /gene="xisR"
                     /locus_tag="OO135_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5398110.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            2712..3998
                     /locus_tag="OO135_002283"
     CDS             2712..3998
                     /locus_tag="OO135_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HCR5398111.1"
                     /translation="MSNASYPTGVEKHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            4019..4720
                     /gene="mlrA"
                     /locus_tag="OO135_002284"
     CDS             4019..4720
                     /gene="mlrA"
                     /locus_tag="OO135_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR5398112.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIRESK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIHDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGYDIFPLGI"
     gene            4780..4887
                     /locus_tag="OO135_002285"
     CDS             4780..4887
                     /locus_tag="OO135_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5398113.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4868..5599)
                     /gene="yehW"
                     /locus_tag="OO135_002286"
     CDS             complement(4868..5599)
                     /gene="yehW"
                     /locus_tag="OO135_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5398114.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5604..6530)
                     /gene="yehX"
                     /locus_tag="OO135_002287"
     CDS             complement(5604..6530)
                     /gene="yehX"
                     /locus_tag="OO135_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5398115.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGELRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPSGTLHFQDLLEEA"
     gene            complement(6523..7680)
                     /gene="yehY"
                     /locus_tag="OO135_002288"
     CDS             complement(6523..7680)
                     /gene="yehY"
                     /locus_tag="OO135_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5398116.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDVIRRISTHPLLRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQIVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7687..8604)
                     /gene="osmF"
                     /locus_tag="OO135_002289"
     CDS             complement(7687..8604)
                     /gene="osmF"
                     /locus_tag="OO135_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5398117.1"
                     /translation="MPLSKVWAGSLVLLAAVSLPLHAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8815..11112)
                     /gene="bglX"
                     /locus_tag="OO135_002290"
     CDS             complement(8815..11112)
                     /gene="bglX"
                     /locus_tag="OO135_002290"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5398118.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNNYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVMNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            11308..13023
                     /gene="dld"
                     /locus_tag="OO135_002291"
     CDS             11308..13023
                     /gene="dld"
                     /locus_tag="OO135_002291"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5398119.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCHVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(13061..13993)
                     /gene="pbpG"
                     /locus_tag="OO135_002292"
     CDS             complement(13061..13993)
                     /gene="pbpG"
                     /locus_tag="OO135_002292"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5398120.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTAPQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(14167..14754)
                     /locus_tag="OO135_002293"
     CDS             complement(14167..14754)
                     /locus_tag="OO135_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5398121.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14924..15502
                     /locus_tag="OO135_002294"
     CDS             14924..15502
                     /locus_tag="OO135_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5398122.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(15632..16393)
                     /locus_tag="OO135_002295"
     CDS             complement(15632..16393)
                     /locus_tag="OO135_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5398123.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNACAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(16446..17882)
                     /gene="mdtQ"
                     /locus_tag="OO135_002296"
                     /pseudo
     CDS             complement(16446..17882)
                     /gene="mdtQ"
                     /locus_tag="OO135_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
BASE COUNT         4541 a         4787 c         4567 g         4152 t
ORIGIN      
        1 gttatgttca accagttact taaacaggta agaaatggca actgactcac aactaaccat
       61 agaaacggcc ctgaatgtcg gcctggcgct ccttggttat ttttacatcg tgttctgcag
      121 cagacggtgg ctgtcgctgt tgttcctgaa aaaatggaat aaacgccgta agcaggatga
      181 acgccagaag gcaatgaatg cgttttccga agccttcgga attgacggca tggaaccagg
      241 ggatccagct cgcgcaatca gcagaggggg tgtagtaatc cttgtatatc ggagtgaaga
      301 gaaaaatgac gatcacaaaa caacgagtag aaaaaatcat atatcgccat gaaatgggac
      361 tgaacagcga tgtcactgcc gaagaggttt atgacctggc tgtactggcg ctgaatttat
      421 caaatatcgc aaacctgaag cgatacgagc ttgatatgga tggttgcgac tcgtgcggtc
      481 aggattgtgg cgcagatatg actgaagatc ctgatggtga ttatgtcctg tttgatgacg
      541 tggttaagtt gtttgaattt gatacaacca ctcaaaagtt agaaatcccg gcaaaggagg
      601 ctgccagtga gcaagattga ctatcaggca ctgcgtgagg cggcagagaa ggctacgaaa
      661 ggtaagtggg ctgttgaatt cgacgatgag atttactcca ctgacggcat aaaccatgag
      721 caaatagcta tggtgttcag tgaaaacgaa tctcgtgatg ctgaattcat cgccgcagcc
      781 aatccggcta ccgtcttggc gcttctggat gaacgggaaa gaaaccagca atacatcaaa
      841 cgccgcgacc aggagaacga ggatattgcg cttacggttg ggaagctgcg cgttgagctt
      901 gaagcagcaa aatcaaaact caacgagcag cgcgagtatt acgagggagt tatctctgat
      961 gggtgcaagc gtattgctga actggaagcg cgggaagttc aattaccgac tcgctacgac
     1021 cttcgatatg gacacccgat aaatgcagat gagcgacaag tcatgatacc taaagaaaat
     1081 ggcagttggc tttacctgat tgacctagaa cacgcattac gcgtcgctga cattcgtatc
     1141 aaaggagagt gatatggcca ctttaacaaa aaaagaacgg gcatggttga atgaattaca
     1201 ggacgttctt gatcgctgtc cattaccaaa aaaaattggt ttttacacca ttggcgataa
     1261 aaccatttac ctgtatgacc tacgccgcat ggatgaaatc atggaggctc ttgataatcg
     1321 ttcgtcgatg gattggtgtg ttgctgtcca tgatatgaat gcagggtttg atgaaaagat
     1381 tttgttcccc tcatcagttg aaagcactgc gggttaagga gtaacacatg accactataa
     1441 ccaaagagcg actgctgaca atcaagcagt ggcgcgaaac atacggaccg ggtagcaacg
     1501 ttgtgcttcc agcagaagaa gcggaagagc tggcacggat tgcgctggca tcactggcag
     1561 cagagccggt ggcaaagatt atagctcatt acccattagg agttgacgta ggcaaacaaa
     1621 aattcgtaca ggccattaga gagcttcctg actttggcgg atatctattt gccgcccctc
     1681 cagcgccgat agtgccggaa gaaatgtata ggcaggatgc gccagttgaa ggcagcagca
     1741 aagcggctgc atacgctaca ggctggaacg attgccgcga agccatgctt cagtccggaa
     1801 actttcggga aaataaagat tcgtcaacca ataattttcg gaaaatcccg gaagcgtcaa
     1861 ccagctctcc ggtaactccg gatggttgga taagctgtag tgagcgaatg ccggacgaca
     1921 ggcaggaggt gaatcaatga gctggcctga tgcaatcgta actctggggg tggtattcgc
     1981 agcagcgttt gttgtgttct cgatttgtcg atggggataa ccacatgttc gctttgattc
     2041 aacgcggtca gatatacacg gacagagccg gataccccgt ggtgattact cgcatcactg
     2101 agcactcagt gttctttcga cggatggacg gacgatccgg gcgggtacgc attggtgagt
     2161 taaactgcct gttcgaacat attgaccacc aggagtaccg caaaattctc gcggacactg
     2221 agcaggaaaa gcacctgaaa aaattacgag ccataaaaag gaagtaaaga atgaataaag
     2281 catttgaacg atgggtccac cagcgttacg gcaatcgcta tgacctgacg cgagatgttg
     2341 acggcttcta ctgtcgtgaa gttgtgaagc gaatgtttga cgtgtggtgc cactgccgtg
     2401 gatgaaaatt ttatgaggtt ggcatgcaga caatcatcta tcagataacc cccagcaaat
     2461 ggtgtacgga gagagtcctc attgcatcaa cagggctaaa gcctggcacc attgagcggg
     2521 caagaagaaa gtcatggatg cagggaaaag aataccgcca ttacgctgta gaaggtgatc
     2581 cggggcacta cagtgaatgc ctgtacaaca tcgaagaaat tatgcgatgg atcgaaaacc
     2641 agaaacaacc aggtgccaaa aatgcaagtt ccggttaacc tgttaatgct cctggacgtc
     2701 tgggaggttt aatgagtaac gcatcatacc cgacaggcgt tgaaaaacat ggaggatcac
     2761 tccgtatatg gtttcactat aatggcaaac gtgtcagaga aaacctcggt gttcctgaca
     2821 cagccaaaaa ccggaagatc gctggtgaac ttcgcacttc cgtttgtttt gcaatcagaa
     2881 tggggagttt cgactacgcc gcgcagttcc ctaattcccc taacctgaaa cactttggtc
     2941 tgggaaaaag agagataacc gttaaggcac tttcggaaaa atggttggac cttaagaaaa
     3001 ttgagatttg tgcgaatgca cttaaccgtt accagtcagt aattaaaaac atgttaccaa
     3061 tgttaggtga aaaaaaactg gtttcatcca taacaaaaga ggatttactt ttcgtaagga
     3121 gagatttgtt gaccggttac caaaagcttt ctaatggaaa gacttcttcc ataaaagggc
     3181 gctcagtggt cacggtaaac tactatatga caaccatagc tggaatgttt caatttgcaa
     3241 cagataatgg ttatacctca ggaaacccat ttaacggtct ggctccctta aaaaagtcca
     3301 aggtaaaacc agatcctctc acccgtgacg aatttattcg ttttattgag gcttgccgtc
     3361 atcaacaaac aaaaaacctg tggattctcg ctgtatacac gggtattcgt cacggggagt
     3421 tggtatcgct ggcatgggaa gatatagacc ttaaagcaag gactataacc atccgtagaa
     3481 attatacaaa acttggcgaa ttcactccac caaaaaccga tgcaggcacc ggaaggacaa
     3541 ttcatctggt tcaaccagct attgatgctc ttaaaagcca ggcggaaatg accatgcttg
     3601 gaaagcaaca ttctgtagag gtgaagcaga gggaatatgg gagaactgct gtgcataaat
     3661 gcacttttgt ttttagtcct caggtaacaa aacagcagca gttgtccgga cctcactaca
     3721 aggttgactc catcagggag tcatggacaa gtatcttaaa acgcgcaggt ctgagacaca
     3781 gaaaatcgta ccaatccagg catacttatg catgctggtc acttgccgca ggagctaatc
     3841 ctagttttat cgcaagccag atgggccaca caaacgcaca aatggtattc aatgtttacg
     3901 gagcatggat gaaagacaac aatcacgaac agatagaact ccttaacaaa agactatctg
     3961 aaagtgtccc atgtatgccc cataagaaag ctgggtaaaa taaaaacttg caaaatcaat
     4021 tagtttaccc ttaatccctg tcacgttacg cgcgtggcag aggcgttacg gattgctgaa
     4081 accgcaacgg acagacggcg gtcatcggct gttcaacgat gccgatattg accggatccg
     4141 cgagagcaaa cgctggatcg acaacggcgt gcaggtcagc aaagttaaaa tgctgctcag
     4201 taatgaaaat gttgatgtgc agaacggctg gcgcgatcag caagaaacat tactgactta
     4261 cttgcaaagc ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg gtcaggatta
     4321 ccccgcccag acgctcacca cgcatttgtt tattcctctg cgtcgacggc ttcagtgcca
     4381 acaaccgact ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc
     4441 catctgtctg gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa
     4501 tattcacgat accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg
     4561 cattgatgtc ctcgcccact cgctcaatca actacgccct gaactgttcg aaggccgtac
     4621 attgctggtg tggtgcggtg aaaatcgaac ctccgcccaa caacagcaac tcaccagttg
     4681 gcaagaacaa ggctatgata ttttcccact cggcatttaa tgattcgtta acaaatgcgc
     4741 tttactgtac aatcctttcg ttaacataag gagtgcatta tgcgcatagc taaaattggg
     4801 gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat gctcgcaggt
     4861 tataccttta ttttgcgtgc tggctaagcg cctgcaccag cctttcaaac aggcggtctg
     4921 cgatgatcgc cgccagtgcc accagtaacg ccccctggat cacatacgcg gtattaaatc
     4981 cgctaagccc gatgatgatg ggcgtaccca gcgtgctggc ccctaccgtt gaggcgatcg
     5041 tcgccgtacc aatgttgata atcaccgaag ttcgcacgcc cgccagaatc accggagctg
     5101 ccagcggtag ctcgacctta cgcagtcgct gaccacgact cattcccata cctttcgcaa
     5161 cttctgtcac gctggcatca atcgctccca gcccggcaag tgtcgcctgc aggacgggca
     5221 gcacaccgta aaggatcaag gcgataatcg ctggttgcag accaaacccg atcaccggaa
     5281 cggcgatcgc cagcactgcg acgggcggaa aagtctgtcc aacggcggca atagtttcca
     5341 ccagtgggcg aaattccgcg ccccacgggc gagtgacagc aattccggca ccagtaccaa
     5401 tgatcaccgc aaacaaactc gaaattccca ccagccagaa atgagccagt gccagagcgg
     5461 caaaactttc ttgctgataa acgggtcgtg gcagttgagg gaacaaggca gcaaacagcg
     5521 gctggctgta aggcagccag aaaatcagtg ccacaaacaa agcaatgagc cagaacagcg
     5581 gatcgcgcaa catcttcata cgcttacgcc tcctccagca gatcctgaaa atgcagcgtg
     5641 ccgctaggct ggccctgcgt gttcaccacc ggcagcacct cgcatccccg cgcgacaaac
     5701 agggagagcg catcgcgcag cgtcatctct tctgccagtg cctcaccttc tgcccgttct
     5761 tcgcgacgca cgtaatccgc cacactacgt aacgaaagca ggcgcacacc cagttcacta
     5821 cgtccaaaaa actggcggac aaaatcattc gccggacgag tcagcatcgt cagcggattc
     5881 ccctgctgca ccacttcacc gtgatccatc aataccagat gttctgccag ccgtagcgcc
     5941 tcatcaatat catgagtgac cagcacaatg gtacgcccca gcaaacggtg aatgcgcgtc
     6001 atctcttgtt gcaacgcgcc gcgcgttacc gggtccagtg cgccaaaagg ttcatccatc
     6061 agtaagactt gcggatcggc agccagtgcg cgcgctactc ccacacgttg ctgctgacca
     6121 ccggaaagct gatgcggata acgctcacgt aaatttgact ccagccccag tagcgccatt
     6181 aattcgtcga tacgatcgtc aatcctcgcc cgcgaccatt tttgtaattg cggcacggtg
     6241 gcaatgtttt gtgccacgct ccagtgggga aacaggccaa tagattgaat ggcatagccc
     6301 atacggcggc gcaactccag tactggcagc gagcgaattt cttctccggc aaagcggagc
     6361 tctccgctgt catgttccac caggcggtta atcattttca gggtggtgga tttgccggag
     6421 ccagatgtgc caatcagcac cgaaaaactc ccttcctgaa aattgagatt gagatcgtta
     6481 acggcttttt gtgcgccaaa cagtttgctg acatggctaa attcaatcat tacgtttcac
     6541 cttcaacagt gcgataagta aatcgaacag cgcatcgatc agcaccgcca gaacaattac
     6601 cgggatcacc cccagcaaca ctaaatcaat ggcgctgctt agcagcccct ggaaaaccag
     6661 cgcaccaaaa ccgcctgcgc cgattaacgc cgcaatcacc gccatgccta caatttgcac
     6721 catcaccacc cgcaggctgc gcagaaatac cggtaacgcc agcggtaact gaacatgcag
     6781 gaatcgctgc gccccgctca tccccatcgc tctggcgctc tccagcacat cgcgcgggat
     6841 ctggttcaag cctactacca cgccgcgcac cagcggcagc aaggcataga gcaccagcgc
     6901 aatcagtgcg ggtgtcattc cggttcctgc tatgccgagc ttccccagcc acggaaaggc
     6961 cgtcaccagc gcggcaagcg gcgcaatcaa caggccaaag agcgccaccg aaggcacggt
     7021 ctgaatgaca ttgagcagag aaaaaatcgc cccctgccgc gcggtggaaa agtagcacca
     7081 gatgcccaat ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca gcaacgtcag
     7141 atgttgagcc agcgcgtcgt caaacacatc ctgacggttg gcgtattctt tcattagcga
     7201 gagatcgtta agcgtgccgg agtaaagcaa ccacagcgga ataatggcaa tctgcatatg
     7261 caacaaccag cgcaacagcg gatgcgtgga gattcggcgg atgacatcgc tacaggccag
     7321 caatgccagc gccgcagcca gccagaaacc actgccgagg ctggtacgcg ccagcgcact
     7381 gccattttgc gccagttggg tcgccgcctt tccagctccc cacaccagca atacgaagac
     7441 gaattgcgcc agaatgagtg caaaaatgct gcctttttta gcgggaataa aacaggctgt
     7501 cagccaggcg caaccaacgc ccaccagcat ccagatcgtt tgcggccaca gttgccagag
     7561 atgacgccct tcacccgaga ctaaacgatt aggcgcgtag ctgataaacg gcagcgctgc
     7621 tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga ttaatacgta gataagtcac
     7681 gggaaattac ttcgcccacc ctttttgttt caggtagtcg gcagccactt ttttggcatc
     7741 cagtccttcc actgcaatgc tggcattcag ttgctgcaat gtttttgcat cgaggctggc
     7801 gaagactggc tgtagccact gtgccatttg cggatactct ttcagcaccg actcacgcac
     7861 cactggtgca ggcgcgtaga taggttgcac gccttgcgga tcgcttaagg tttgcagccc
     7921 cagcgccgcg accggaccgt cagtgccgta agccattgcg gcattaacgc cagaagtttg
     7981 ctgggcagcg gctttaatcg tcaccgccgt gtcgccacca gccagtgaca gcaactgatc
     8041 ctgaccgagc ttaaagccat aggctttttc aaacgcgggt aaggcatcgg cgcgttcgat
     8101 aaactctgcc gaggctgcca gtttgaaggt gccgccctct ttcagataac gactcaggtc
     8161 agcaagcgaa gtgagtttgt ttttctctgc cacatcctga cgcacggcga tggtccaggt
     8221 gttatttgca ggtgcgggcg tcagccagat taacttgttt tgctctgcat cgagtttttt
     8281 gactttctcg taaccttgct gcgcgttttt ccacgctgca tcgttttcat ctttaaagaa
     8341 aaacgcgcca ttgccggtat attccggata gatatccagt tcaccagaag taatcgcccc
     8401 gcgcaccaca ggagtcgttc cgagttgcac tttattgacc gtcggtacgc cgtggctttc
     8461 cagtacctgc aaaatgatat tgccgagcag cgcgccttca gtatcgattt ttgaaccgac
     8521 tttaacgggg gaagccgcgt gcagcggcag gctcacggct gccaacaaaa ccagtgaacc
     8581 tgcccagacc tttgagagtg gcatgatgct ttcctcattc ttttactgtt gttttccgcg
     8641 aattaagaga aaagcatagt tgataatggc gggattagcc tgagcggacg gattcagttg
     8701 cagaatcaga taaatactta agaggcatat tcggtccggc gtttcctgcc ggatgcggcg
     8761 cgagcgcctt atccggccta caaagggcgc aaatgtcacg cccttactaa agcattacag
     8821 caactcaaac tcgcctttct taacgcgtgc agaatcagtg ccgataaaga cattgaactt
     8881 gccaggctcg gcgtcatatt tcatctgttg attccagaac ttcagcgcct cgatatcaat
     8941 cgggaagcta acggtctgag tttcgcccgg tttcagggtg attttctcaa agcctttcag
     9001 ctgtttcacc ggacgactca tggaagccgt cacatcctgc aagtacatct gcactaccgt
     9061 ggccccttcg cgtttaccgg tgttcatcac ctgcacgctg gccgtcactt tgccgtcacg
     9121 cttcatggtc ggcgcagaaa gtttcacatc agagacggtg aaagtggtat agctcagacc
     9181 atagccgaac ggataaagcg ccccgttagc ttcatcaaaa taacgcgaag tgtatttgtt
     9241 cggcttgtcg gcattatacg ggcgaccggt gttcagatgg ctgtagtaca ccgggatctg
     9301 cccgacagaa cgcgggaagg acatcggcag cttgccggac gggttgtaat cgccaaacaa
     9361 cacatcggca attgcattac cgccttcagt ccccgcaaac caggtttcca gaatcgcatc
     9421 agcctgctga tcttctttca ccagcgccag cggacgcccg ttcatcagca ccagcaccag
     9481 cggtttaccg gtggctttca gcgccgcaat caagtcacgt tggctttgcg gaatagtaat
     9541 atcggtacgg ctggaggcct cgtgcgccat cccctgtgct tcaccgacta cagccaccac
     9601 cacatcagat tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg gcgaacgcgg
     9661 atcgactttg accgcttctt catactgatt caggaaatcg ataatgcctt tgtcactggt
     9721 aacgttcgcc cctttggcat acagcacttt accgttttca ccgacggcat ttttaatccc
     9781 ggtcagcacg gtcacggatt gatctgcaac acctgccgcc gaccagcttc ccatcacgtc
     9841 acgcttgctg tctgccagcg ggccaaccac cgcaatggtt gcggattttt tcagcggtaa
     9901 cgtttcgaga cggtttttca gcaacaccag gctttcacgc gccacttcac gcgcttcttt
     9961 gcggtgcaag cggctttcgg cattggtatc caccgggtca gactctttcg gaccgagatg
    10021 gctgtatggg tcgttaaaca accccatatc atatttaacg ttcagtacat ggcgggcagc
    10081 atcgtccagc tctgccatcg tcactttacc ggatttaatc aacccaggca gatacttcga
    10141 gtagtactcg tcgctcatac tcatgttgat tccggatttc agcgccacgc gcaccgcatc
    10201 ttccgggtct gccgccgtgc catgtttaat cagctctttg attgcaccgt gatcggaaac
    10261 ggtgatgcct ttaaagcccc actggtcgcg cagaacatct ttcagcagcc aggaatcgga
    10321 ggtggctggc gtgccgttca gcgagttcag cgccaccatc accgcgccgc tgcctgcgtc
    10381 cagccccgct ttgtacggcg gcatataatt attaaacagg cgctgcggac tcatatcgac
    10441 ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt tgacgctggt
    10501 catcaccgag tagcgatctg ccgggctttt accctgcatc gcttccacca tggttttacc
    10561 cattgttgag gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc cccagcgcgg
    10621 atcgcgcgag acatcgacca tcggtgccca ggtcatattc aggccatcat ctgccgcttc
    10681 ataagcagag acacgtccga ccgttttcac tgcatcgagg ttaaaagacg aggccagacc
    10741 gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa agaaaagagg
    10801 aattttcagg cggcttaatt ccatcacctg atcctgcatg gcgcggatat cctgacgggt
    10861 tacggtgttg aaaatcgccc caacctgacc gtctttgatc atctcgcgga tcgcctcttt
    10921 cgggttatcc gggccgacgc tgattaagcg cagctgacca attttctcat caactgtcat
    10981 tttcttaagc agttcggtga cgaacgcatc ccgcgcttcg ggcgttaatg gatggttgcc
    11041 gaataaatca tccgccagtg ctggctgcag ggccagactc accgcgattc ctactgaaca
    11101 tagccatttc atatggattt ttcttcctct gttgcccgac gaggcagcga aaatggtgca
    11161 aaaaccgtag tttgccataa gcatgatgga gagagaaaaa gaatgctcag tttattgtct
    11221 gaattttcaa aatattcact cgctgaattg tcatacaagg cgctattcta gtttgtgata
    11281 ttttttcccc accacaagga gtggaaaatg tcttccatga caacaactga taataaagcc
    11341 tttttgaatg aacttgcccg tctggtcggt cattcacacc tgctcaccga tcccgcaaaa
    11401 acggcccgct atcgcaaggg cttccgttct ggtcagggcg acgcgctggc tgtcgttttc
    11461 cctggctcac tactggaatt gtggcgggtg ctgaaagcct gcgtcaccgc cgacaaaatt
    11521 attctgatgc aggctgccaa tacaggcctg accgaaggat cgacgccaaa cggtaacgac
    11581 tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg acaagctgca cgttctcggc
    11641 aagggcgaac aggtgctggc ctatccgggc accacgctct attcgctgga aaaagccctc
    11701 aaaccgctgg gacgcgaacc gcactcagtg attggatcat cgtgtatagg cgcatcggtc
    11761 atcggtggta tttgtaacaa ctccggcggc tcgctggtgc aacgtggccc ggcgtatacc
    11821 gaaatgtcgt tattcgcgcg tataaatgaa gacggcaaac tgacgctggt gaaccatctg
    11881 gggattgatc tgggcgaaac gccggagcag atccttagca agctggatga tgatcgcatc
    11941 aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg attatgacta tgtccaccgc
    12001 gttcgtgata ttgaagccga cacgcccgca cgttataacg ccgatccgga tcggttattt
    12061 gaatcttctg gttgcgccgg gaagctggcc gtctttgcag tacgtcttga taccttcgag
    12121 gcggaaaaaa atcagcaggt gttttatatc ggcaccaacc agccggaagt gctgaccgaa
    12181 atccgccgtc atattctggc taacttcgaa aatctgccgg ttgccgggga atatatgcac
    12241 cgggatatct acgatattgc ggaaaaatac ggcaaagaca ccttcctgat gattgataag
    12301 ttaggcaccg acaagatgcc gttcttcttt aatctcaagg gacgcaccga tgcgatgctg
    12361 gagaaagtga aattcttccg tccgcatttc actgaccgtg cgatgcaaaa attcggtcac
    12421 ctgttcccca gccatttacc gccgcgcatg aaaaactggc gcgataaata cgagcatcat
    12481 ctgctgttaa aaatggcggg cgatggcgtc ggtgaagcca aatcgtggct ggtagattat
    12541 ttcaaacagg ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag caaagcgttt
    12601 ttacaccgtt tcgccgctgc gggcgcagca attcgttatc aggcggtgca ttccgatgaa
    12661 gtcgaagaca ttctggcgtt ggatatcgct ctgcggcgta acgacaccga gtggtatgag
    12721 catttaccgc cggagatcga cagccagctg gtgcacaagc tctattacgg ccattttatg
    12781 tgccatgtct tccatcagga ttacatagtg aaaaaaggcg tggatgtgca tgcgttaaaa
    12841 gaacagatgc tggaactgct acagcagcgc ggcgcgcagt accctgccga gcataacgtc
    12901 ggtcatttgt ataaagcacc ggagacgttg cagaagttct atcgcgagaa cgatccgacc
    12961 aacagcatga atccggggat cggtaaaacc agtaaacgga aaaactggca ggaagtggag
    13021 taaaaattac ggatggcaga gtatcgccat ccgaattcac ttaatcgttc tgtgccgtct
    13081 gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggca
    13141 caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg
    13201 tatatttgcc aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtac
    13261 gcatcaccag acaatggccc gccgcattgg taaagccggt tttggttaac tgaatattcc
    13321 agttatcgcg atacaccaga tgattagtat tgcggaacgg cagcgtatac gtcgggttag
    13381 agaaggttgc catatcttcc cgggtagtac ttaactgccc gatcaacgga tattgtttgc
    13441 tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc gacaatccgg
    13501 taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca ttcattgcct
    13561 taataaaggc tttgtaacca ccgggataat ggtgcgcaag gcttgccgcc gcgcggtttt
    13621 ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta ttcagtcgta
    13681 cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt agtttttcat
    13741 ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa
    13801 tcggacgcac cagatccggg tggttcgaat agatcacttt gttggtattc agatcaacaa
    13861 tcatcgcgct accggaggca atttccggtt gtggagcggt ggtagcggct gccgttttcg
    13921 caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc
    13981 gaaatttcgg catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg
    14041 gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa
    14101 aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc aacgtcaaag
    14161 aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg cgagcagcac
    14221 ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc cttcctcttt
    14281 attgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt aaaacagcgc
    14341 caccgtgccg accagcgcac acaaccagac cagcggataa agcgccacca gaccgcttaa
    14401 aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg
    14461 cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc cagccagcat
    14521 aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact tcaggatagt
    14581 gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat
    14641 cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt tttcgcgatt
    14701 aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc
    14761 cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt
    14821 tgttcatcca tgtaatttgc accgtctgat gtatgtcctt tttcagcgtg aatgatttcc
    14881 agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc tcaatacact
    14941 catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt
    15001 gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc tggtggtact
    15061 ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg ggcggcggtt
    15121 tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa
    15181 acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt atggctttcg
    15241 ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccgct
    15301 gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt acgctggtgg
    15361 tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact gggtctggtt
    15421 gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc gcgggaagaa
    15481 aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg ccgcatccga
    15541 catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta ccaaaatttt
    15601 acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc ggattcgcca
    15661 acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg ccctctgagc
    15721 acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc agaggaatcg
    15781 aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc gtggcgatcg
    15841 ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc gccatcgctt
    15901 tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc ggcagcggcg
    15961 tatgttcatg taccgacgta atgttgatga tgcgaccgcc ctgcccttgt ttcaccatct
    16021 ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta aaaatcttgc
    16081 gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgcac gcattattca
    16141 ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc gcaccttctg
    16201 gcagtttacc gagatccagc tgcacaatct ccgcacgtac gccgtggcta actacctcac
    16261 gcgcagtatc ttttgcccct tcttcatctg agtgccaggt aataccaata tcaaacccct
    16321 gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg gtaataatcg
    16381 caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat agaaggcgca
    16441 tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga agcatccagc
    16501 cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat tctggcttcg
    16561 ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag acctaccaca
    16621 cgcaaggcat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag tgtctgaacc
    16681 tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt gtagctggtg
    16741 atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg accactatcg
    16801 aaaataggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg acggaacaga
    16861 tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc aggataaaat
    16921 gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg cgccgcctgc
    16981 aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg gcttgccact
    17041 ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggtgatttgt aagtgcgctt
    17101 aaccgtgcct caataatttt cattttcccc gcgacatcgt tgagctgctg ccgggttttg
    17161 ctggcattaa tatcggtttc cacaccgtca actgaagaag taatcccgtt ctgatatagc
    17221 tggcgatcgg tcgcgataat ggtgttctgc tctttttcta tttgctgcaa gaccgtgttt
    17281 aacgccgcct gggtttgcca ctcccagtac aggcgggcta cgctgccagc cagcaattgg
    17341 cgggtttgct cgcgttccgc cgcccgtgct ttaaccgtac ccaggcgggc agtaacctcc
    17401 gcccgattct ttccccagat atcgagatgc cagcccgccg ttaagccaaa agttccgttg
    17461 gtgtaccacg gaccggtcgt acctgcggcc ggatcgttca gagcaaacgg ccccattagc
    17521 ccttctgccg acattttttg ccgctccata tccgcctaaa agtcgatctg cggaccatcc
    17581 tgagtggcaa ctgccttcgc ctgggcttca gctagctgaa tgcgctgttc agccacctgc
    17641 atatccggtg cgttctgtag tgcattgtta attaatgaag tgagctgatt atcgtgatac
    17701 tccagccacc attggctgtc tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca
    17761 acttgtgcag cgggcgtttg ctggcttaac gcctggcggg tttcatgcat aggcgcacac
    17821 ccggccagca tcagtaacag cggaaaacag gcgatggctg gataaaagga atcacgattc
    17881 atgggggaat aatcaggtaa gaaacggtgc gcggtgatta ccgtgtgttg cgatatattt
    17941 tttagtttcg cgtggcaata catcagtagc aataaaacga catacccaga aaaatataga
    18001 ctaagtgatt gatattctcc gatttatctt aatcacttat aaatgac
//
LOCUS       DAJTMK010000081        17867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTMK010000081 DAJTMK010000000
VERSION     DAJTMK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17867
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.082"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            274..840
                     /gene="yqaB"
                     /locus_tag="OO135_002297"
     CDS             274..840
                     /gene="yqaB"
                     /locus_tag="OO135_002297"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5398124.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQTIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            837..1250
                     /locus_tag="OO135_002298"
     CDS             837..1250
                     /locus_tag="OO135_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5398125.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFCALVKRYAMLQLQQLPFRA"
     gene            1337..2893
                     /gene="gshA"
                     /locus_tag="OO135_002299"
     CDS             1337..2893
                     /gene="gshA"
                     /locus_tag="OO135_002299"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5398126.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3043..3558
                     /gene="luxS"
                     /locus_tag="OO135_002300"
     CDS             3043..3558
                     /gene="luxS"
                     /locus_tag="OO135_002300"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5398127.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3622..5160)
                     /gene="emrB"
                     /locus_tag="OO135_002301"
     CDS             complement(3622..5160)
                     /gene="emrB"
                     /locus_tag="OO135_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5398128.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPDAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5177..6349)
                     /gene="emrA"
                     /locus_tag="OO135_002302"
     CDS             complement(5177..6349)
                     /gene="emrA"
                     /locus_tag="OO135_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR5398129.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVAANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6476..7006)
                     /gene="emrR"
                     /locus_tag="OO135_002303"
     CDS             complement(6476..7006)
                     /gene="emrR"
                     /locus_tag="OO135_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5398130.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7097..7432)
                     /gene="ygaH"
                     /locus_tag="OO135_002304"
     CDS             complement(7097..7432)
                     /gene="ygaH"
                     /locus_tag="OO135_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5398131.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7422..8159)
                     /gene="ygaZ"
                     /locus_tag="OO135_002305"
     CDS             complement(7422..8159)
                     /gene="ygaZ"
                     /locus_tag="OO135_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5398132.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRYVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8283..9467)
                     /locus_tag="OO135_002306"
     CDS             complement(8283..9467)
                     /locus_tag="OO135_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398133.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9759..10751)
                     /gene="proX"
                     /locus_tag="OO135_002307"
     CDS             complement(9759..10751)
                     /gene="proX"
                     /locus_tag="OO135_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5398134.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10809..11873)
                     /gene="proW"
                     /locus_tag="OO135_002308"
     CDS             complement(10809..11873)
                     /gene="proW"
                     /locus_tag="OO135_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5398135.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTTLLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11866..13068)
                     /gene="proV"
                     /locus_tag="OO135_002309"
     CDS             complement(11866..13068)
                     /gene="proV"
                     /locus_tag="OO135_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5398136.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13423..14382)
                     /gene="nrdF"
                     /locus_tag="OO135_002310"
     CDS             complement(13423..14382)
                     /gene="nrdF"
                     /locus_tag="OO135_002310"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5398137.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KNFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14392..16536)
                     /gene="nrdE"
                     /locus_tag="OO135_002311"
     CDS             complement(14392..16536)
                     /gene="nrdE"
                     /locus_tag="OO135_002311"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5398138.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16509..16919)
                     /gene="nrdI"
                     /locus_tag="OO135_002312"
     CDS             complement(16509..16919)
                     /gene="nrdI"
                     /locus_tag="OO135_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5398139.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFSEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16916..17161)
                     /gene="nrdH"
                     /locus_tag="OO135_002313"
     CDS             complement(16916..17161)
                     /gene="nrdH"
                     /locus_tag="OO135_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5398140.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17409..17738)
                     /locus_tag="OO135_002314"
     CDS             complement(17409..17738)
                     /locus_tag="OO135_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5398141.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALMKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
BASE COUNT         4446 a         4552 c         4653 g         4216 t
ORIGIN      
        1 cgtaaccttc agcaatgaag gtatcgaaga gatagcggta ttaaccgcag gcactaggga
       61 ggataacgtt gcgtcagcaa cggcccgaag ggcgaacgcc agtgagtcat cctcccggat
      121 gcaccatctt ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat
      181 ccatttcctg aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa
      241 gtagcgtcat atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt
      301 tttgatatgg atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa
      361 gtattagggc actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg
      421 cccacctggc gtattgctca gacaattatt gagctgaatc aggccgatct cgacccgcat
      481 gcgttagcgc gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg
      541 cttcctcttg ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg
      601 gggagtgaaa gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt
      661 gacgccgtcg tcgctgctga tcacgtcaaa caccataaac ccgcgccaga cacatttttg
      721 ttgtgcgcgc agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat
      781 ttcggtattc aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga
      841 gtgaagcgtt atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac
      901 ccggcaactc cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg
      961 ttttagtttt aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg
     1021 ggcgtttctt tccattgcgt aaaacatcgc gctggcaaga gaaagctact ggctggctga
     1081 aacgctatgg tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt
     1141 gtctgttagc gggatggatg cgcatctcgt ggggaccggt aatctttttt tgtgccttgg
     1201 taaagcgtta cgctatgttg cagttgcagc agctaccgtt cagggcatga tgtggtggca
     1261 ctaattgtag gcctgcacat atggtcacca ttacagttat gctaattaaa acgattttga
     1321 caggcgggag gtcaatttga tcccggacgt atcacaggcg ctggcctggc tggaaaaaca
     1381 tcctcaggcg ttaaagggga tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc
     1441 tgatggcaca ctggcaacaa caggtcatcc tgaagcatta ggttccgcac tgacgcacaa
     1501 atggattact accgattttg cggaagcatt gctggaattc attacaccag tggatggtga
     1561 tattgaacat atgttgacct ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg
     1621 cgatgagcgg atgtggccgt taagtatgcc atgctacatc gcagaaggtc aggacatcga
     1681 actggcacag tacggcactt ctaacaccgg acgctttaaa acgctgtatc gtgaagggct
     1741 gaaaaatcgc tacggcgcgc tgatgcaaac catttccggc gtgcactaca atttctcttt
     1801 gccaatggca ttctggcaag cgaagtgcgg tgatatctcg ggcactgatg ccaaagagaa
     1861 aatttctgcg ggctatttcc gcgttatccg caattactat cgtttcggtt gggtcattcc
     1921 ttatctgttt ggtgcatctc cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc
     1981 gctgccgttt gagaaaaccg agtgcggtat gtattacctg ccgtatgcga cctctcttcg
     2041 tttgagcgat ctcggctata ccaataaatc gcaaagcaat cttggtatta ccttcaacga
     2101 tctttacgag tacgtagcgg gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc
     2161 gaagattggt attgagaaag acggtaagag gctgcaaatc aatagcaacg tgctgcagat
     2221 tgaaaacgaa ctgtatgcgc cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc
     2281 ttctgatgcg ctgttacgtg gcggcattga atatattgaa gtgcgttcgc tggacatcaa
     2341 cccgttctcg ccgattggtg tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt
     2401 ctggtgtgcg ctggctgatg caccggaaat gagcagtagc gaacttgcct gtacacgcgt
     2461 taactggaac cgggtgatcc tcgaaggtcg caaaccgggc ctgacgctgg gtatcggctg
     2521 cgaaaccgcg cagttcccgt taccgcaggt gggtaaagat ctgttccgcg atctgaaacg
     2581 cgtcgcgcaa acgttggata gcattaacgg cggcgaagcg tatcagaaag tgtgtgatga
     2641 actggtcgcc tgcttcgata atcccgatct gactttctct gcccgtatct taaggtctat
     2701 gattgatact ggtattggcg gaacaggcaa agcgtttgca gaagcctacc gtaatctgct
     2761 gcgtgaagag ccgttggaaa ttctgcgcga agaggatttt gtagccgagc gcgaggcatc
     2821 tgaacgccgt cagcaggaaa tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga
     2881 aaaacacgcc tgacagaaaa gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga
     2941 attcagggat gatgataaca aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa
     3001 agagttcaga aaatttttaa aaaaattacc ggaggtggct aaatgccgtt gttagatagc
     3061 ttcacagtcg atcatacccg gatggaagcg cctgcagttc gggtggcgaa aacaatgaac
     3121 accccgcatg gcgacgcaat caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa
     3181 gtgatgccag aaagagggag ccatactctg gagcacctgt ttgctggttt tatgcgtaac
     3241 catcttaacg gtaatggtgt agagattatc gatatctcgc caatgggctg ccgcaccggt
     3301 ttttatatga gtctgattgg tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg
     3361 gcaatggaag acgtgctgaa agtgcaggat cagaatcaga tcccggagct gaacgtctac
     3421 cagtgtggca cttaccagat gcactcgttg caggaagcgc aggatattgc gcgtagcatt
     3481 ctggaacgtg acgtacgcat caacagcaac gaagaactgg cactgccgaa agagaagttg
     3541 caggaactgc acatttagtc agtaaactat cttcacaatt aattgaaaaa agccagttca
     3601 aatgaactgg cttagttgta cttagtgcgc accacctccg ccgccacctg cgccaaaggg
     3661 cggtttagca aaccacacca gccccagcag gacgaggaat atcccggctg acatccagaa
     3721 gatctcattg gcggaaataa tcagcccctg attagtgatc tgctgggcaa tccagcctga
     3781 tgcctgctgt tgcgtcatcc caagcccttc cagttgactg tacatcgcct gggcatccgg
     3841 gttgaatggg tttaccgact cagtcaactg cgcatggtgc atcgactcgc ggttggtcca
     3901 catggtcgtg gttatcgacg tgccgataga ccccgccagc gttcgcgtaa agttagagag
     3961 gctcgatgcc gccgccagtc gttccggtgg caaaccagac agcgtaatgg tggtcagcgg
     4021 cataaagaag caggccaccg caaacccctg gataaactgc ggccaggccg acgcgccaaa
     4081 atccatacct ggttcaaagg tataggcacg ccagtagaag cagacggcat acataataaa
     4141 gctgaaggtt accagccgcc gcatatccag tttatgcgcg aagcggccga taatcggcga
     4201 caggatcacc ggaataatcc ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta
     4261 accgtagacc tcctgcaaca actgcggcag cagaacaatc gcgccgaagt agagcatata
     4321 cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca acgacagatc
     4381 gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag ccaccaccgc
     4441 caccacggta agaatgataa tttcctgtga tgaaaaccag tccagctctt taccgcggtc
     4501 gagcataatc tgcaggctac cgataccaat aaccagcagt gccagcccca cggcatcaat
     4561 ccgccgccgt tcggtgcggg tttcgcgtcc gcgcagggtt tgcagtgtca tcaacaccac
     4621 cgccacgcca attggcacgt tgatgaagaa tatccagccc cagtggtaat tatcgctgat
     4681 ataaccgccg aggattgggc cgcaaattgg cgcgacaatc accgtcatcg accacaacgc
     4741 cagcgcgatt gagcgtttgg ctggcgggta attattcagc aaaagacttt gcgaaagtgg
     4801 gatcaacggc ccagcgacaa tcccctgaat cacacggaag aagatcagca tattcaggct
     4861 gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc aaaggaacag
     4921 tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga tggcattcgc
     4981 caccccgaaa gaagtgatta cccacgtacc ctggctgagc gatgagccca gattcccggc
     5041 gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga atgtcgccag
     5101 tgacagcgca atcgtcataa tgaccagttg cgcgccttcc agcggttttt gctgttgcat
     5161 cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc agtttattga
     5221 caggtgccag gctgatttca cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat
     5281 ttgccagtac ctgaccgtca cggttagtgg tattgacgct caccagcgtg gacaaaccaa
     5341 tgcgcagcgg gtattgctcc agctgtttct ggtccagttc gatacgcaca ggtagacgct
     5401 gaacgacttt gatccagtta ccggtcgcat tttgcgccgg aagcagtgag aatgcgctac
     5461 ctgtgcccat atccagacca accactttac cggtgtattt cacatcatcg ccgtaaatat
     5521 ccgtggtgat agtgaccggc tgaccgatac gcatattggc aatctgcgtc tctttaaagt
     5581 tggcagccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc gttgggctaa
     5641 tttgcgcccc aggctgtacc gcgcggcggg agacataacc ggtcatcgga ctgacaatac
     5701 gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt tgcacggcag
     5761 gctgatcttc cagtttagtc cccagaatca tcgcctgatt ggcattgtat tgttgaatag
     5821 cgacgtccag ttgcgcctgg gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc
     5881 gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt tgtgctttcg
     5941 caagggcgat tttttgcacc tcaatattcg cctgtaactg tttgctgtta atcatcagct
     6001 ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac gcctggcgag
     6061 catctgtcgg gtcgagagtg accagaacat cgccttcttt tacaaaatcg gtgttatcgg
     6121 cccagacttt cgttacgctg ccagacacct gagacataat ttgaatctga ttccctgcca
     6181 cgtatgcgtc atcggtttct tcgaaatgac gcagtaccaa aaaccaataa atccctatcg
     6241 ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta cgtttgccgc
     6301 tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata ttgttctcca
     6361 cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag catgttgcga
     6421 tgctggccgg tcattttttc ttttataaat ctggattttt gagcgagatg acgcgttagc
     6481 tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc gagacgggag agcaatttgc
     6541 gggtgatttg ctcgagctga tctttttctg ttgtgctgag cgcggaccag agttgatgca
     6601 ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt
     6661 gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt
     6721 ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca caacttaatt
     6781 cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc ataaacaacg
     6841 tctcgttaat cccctgagcc ttcagcattt tattgcggtt ctccagcagc ttgctttgca
     6901 tgtgcatgca aagacgagtc agaaggatct cctgataagg aaaatcttcg tggcggctgg
     6961 cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg gtatgacctc
     7021 attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat tgttttagaa
     7081 aaatgattct tgtgggttat ataatcgcca tcactttcca ggcgagccca taggccagcg
     7141 cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca cccagtaccg
     7201 cgaagccgac cagcgtgggc acgaaacggc gtgtatcgtg catcacttct ggtgcggtag
     7261 agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa atacctaccg
     7321 cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaaa tagcggaagc
     7381 aataattcgc cacgccaacc agtaacccaa gcagcagaac ctcatagctc atcgggcgct
     7441 ccttgccaga atgcctggat taacgcagtg aggcagccac agacaatgcc tgccagaatg
     7501 gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc tgcggtaacg
     7561 caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag tgccggaagc
     7621 ataaaaccta atgcagcttc aacggcggga taaccttgca gcaagccgct gccggagaat
     7681 gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat gccgatcatc
     7741 cagttctcgc tccagcggcg attattgcgt accagttttg cggttgcggc ggcaaaaacc
     7801 tcatccgtca ggccaaatgc ccacagggcg gttttcgatt tttgcagacg ctgaataata
     7861 cggctacgca gtgacgggcc atacaacaca tagcgaacat ccattgccat gacggtcagt
     7921 gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg
     7981 cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc
     8041 gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg taaactgtct
     8101 ttgcatcctt ccatgaaggt cgccgaacca agagcaggct gtggagtagg gctttccata
     8161 gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc ttaaccttat
     8221 cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg gttgctgatt
     8281 gcttaatttg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa gttaaccagg
     8341 gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc catgttgcca ggctgaggct
     8401 gaaattagcg aaccggcggc accgccaata aagtagctgg tcatgtaacc tgcggtcagg
     8461 cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat atgcacgccc
     8521 tgtacggtga gatccagcac caggatgccg ataatcagcg ccagtacgga agtgtgacca
     8581 aaccagatcg ccagccatga aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc
     8641 gatttaccct tatcggcaaa accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc
     8701 gcaagtccaa acagaccaat gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa
     8761 aaggccattg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg
     8821 cgggtgcgca ggatcttatc gctgataaac atgctgaaaa cggaacctaa caactgtggg
     8881 tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc cagcgccatc
     8941 agtgccatta acaccgaggc aacccaaaag acggtgcgcc agccgccgag attcgccagc
     9001 aatccggcaa cggtccgtgc gagtaagatc cccaacagca gaccgctcat aatagtgcca
     9061 accactttgc cgcgtttgtc cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt
     9121 gccacgacag agaataaacc ggttaatgcc gtaccgagga tcatcatcgc cagcgactga
     9181 ctgctggcag tgattaacat gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg
     9241 cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgctgc atagcccaat
     9301 tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg
     9361 gtgtcgagca atggctgggc gtaatagtta ctggctaccg ccagaccggt ggcgatagac
     9421 atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat ttttgttcat
     9481 caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatatg ggatgaaaga
     9541 tagcttgttg aaatgtgcga tgtggtttgt aggcatgata agacgcgcca gcgtcgcatc
     9601 aggcattgtg cacagaacgg cggatgcggc gaaaacgcct tatccgccat acggaatgag
     9661 tgtattttgt aggcatgata agacgcgtca gcgtcgcatc aggcattgtg tacagagcgg
     9721 cggatgaggc aaaaacgcct tatccgcctg aataaaaatt acttctgcgc tgccagcgcc
     9781 tcattcaccc agccatcgaa ctgctgctgg tgggctttga tccagccatc aacgtggccc
     9841 tgaatatcgc cttctgaggc tttgccgtca tgcataatgg cgttctgggc gttaatatcg
     9901 gccactggca actgcataat ggcaaacagt ttggctgccg ccggattttt ctcggtccag
     9961 gctttgttgg caacgatatg catggtgctg accgggaagc cgtaattcgc gccattcggc
    10021 agtttggtat cggcattttt atcgcccggc agtgcggaga acggcacctg caaccagacc
    10081 acatctttcc ctggcttcag ctcattactc acccagtacg gcgtccaggt gtaataaaac
    10141 accggtttgc cctctttgta gcgactgatg gtgtcggcca tcatcgctgc gtagttcccc
    10201 tgattatgcg tcacggtgtt ggtcagttca tacgcggcaa gctggtggtt gatcgcacct
    10261 tcgcagcccc agccagggtt acaaccggtt aaatccgctt taccgtcgcc gttggtatcg
    10321 aacagtttgg cgatcttcgg atctttcagt tgtgcgatgt tggtgatttt gtactggtcg
    10381 gcggttttct tatcgatcag gtaaccctgt gccgcgccgt taacaaatac cccttcacga
    10441 taaaatttct tatcgccacc ggcagcttcg tacatgttgt catgcagtgg cgtccagttc
    10501 acggcggtga aggttgcatc gccggaagca agcgaggtgt agccaacgtt gtaatctact
    10561 tcgctgggtt tgttgacggt atagcctaat ttctccagcg cacggctgac cagcagcgtc
    10621 tggaaggttt cttcggtgat ggtgctttga actggattaa cagtaatacc tttgcccggc
    10681 agatcggcag caaaagtttg tgtagagata agcgtggcaa acgctgtcgc aaaaagtacg
    10741 ctatgtcgca ttgttattcc tttttttggc agggtgataa tgcccggcgt caccgggcaa
    10801 gtgcagagtt acttaatgaa tgggcgggtc agcagaccaa cagggccagt ggtgtaccag
    10861 cgacggttgc cgcgactgcg tgagtcgcgc ccaacggcct gcgtcaggcg atcgaggata
    10921 atggcgagga tcacaatccc gacgccgcca acggtggcaa gccccatatc cagacgaccg
    10981 ataccgcgaa gtaccatctg acccagtccg ccgacggcaa tcatcgaggc aatgaccacc
    11041 atagaaaggg ccagcatcag cgtctggtta acgcccgcca taatggtcgg catcgccagc
    11101 ggtaactgaa ctttgaacag catctggcgc gggctggcac cgaatgagcg cgaggcttca
    11161 atcagatccg ccggaacctg gttaatcccc agaatggtca gacggataat cggcggcaga
    11221 gcaaagatga tcgtcaccac cacgcccggc acgttaccga taccaaatag catgacgatt
    11281 ggcaccagat aaacaaacgc tggcgtggtc tgcatggcat caagcagtgg acgaataatt
    11341 ttcgccgctc gcggacttct cgccagccag atccccagcg gcaaaccaat gacgatacag
    11401 aacagcaggg tggttaacac cagcgccagg gtaaccatgg cctgcgacca ggcaccgatt
    11461 gcgccgatgg caatcagcga aaccagcgtc gccacgccca ttccgacccc ggaaatctgc
    11521 caggcgatga gagcgaaaac gataatcgcc accggtgcgg gcatacccag cagcaattgc
    11581 tggaaaccgt tgaggatata atcaaccgga acgcgcacgc cctggaagac gggacggaaa
    11641 tgggtaacaa cccagtcgat cccttcagtg acccaactgt cgagcgggat cagcgtttta
    11701 tgaaacggat cgagaatatt aaaatgctcg acgtttggcg caggcgtact ggtcagccag
    11761 tcagcaccac cgccgtcagt cggtgcagtc gccggtgtac cccaggcgtc tgcggattgc
    11821 acggcactgt ccgccgctgg cgtggtatcc cacggattat tttgatcagc cattatttac
    11881 cccctcacga tctaaagcgc gcagcagcat tcctttcgaa atgatgccga catactgttg
    11941 gtcctcgtcg accacgggca ccgcacaggg tgcctgtccg acatgagaga gcaactcgct
    12001 aagaggcgtt tgtgcatcga ctgctaacgg cgcatcaatc agcgccgcat caagaccttg
    12061 ctgctgcgtt aacgcggttt taagcgaatc gatggaaact gcgccgacaa acttattacc
    12121 gcgttcgata acgtagccat attcgcgatc ttcatcctgc aataatttca gtgccgaacg
    12181 tgggccgaag ccaggggttt tacgaattaa gccattcggt gtccggcggg caatatcttt
    12241 cgcactgaat acctgactaa tatcaacgcc acggaagaag gtacggacat aatcattcgc
    12301 cggattattg agaatttcat ccggtgtgcc gacctgtacc acttcaccat tttgcataat
    12361 ggcaattcgg tcgccaatac gcatagcttc atcgagatcg tgggaaataa agacaatggt
    12421 gcgctgatgt ttcgcctgta attttaccag ctcatcctgc atctcggtgc gaattaatgg
    12481 atcgagcgcc gagaaggctt cgtccattaa taatatatcc ggattaatcg ctaacgcgcg
    12541 ggctaatccc acacgttgac gcatcccgcc agagagttca tccgggtagc tgtgggcata
    12601 attttccagc ccgacctgac gcagtgcatc aagggctttt tcccggcgtt cttcggcatt
    12661 aattccggcc aattccatac cgaacgcagt attgtccagc acggtcatat gcggcattaa
    12721 ggcaaaggac tggaagacca tcgcaatctt ttttctgcgc acctcacgga gttcggcatc
    12781 ggatattttg gcaatatcca caccatcaat cagcacttgc ccgcgggtgg gttcaatcag
    12841 gcgattgaga aggcgtacca ttgtggattt acccgagccg gataatccca tgatgacaaa
    12901 tatctcgcct tcttcaatgg ccagactggc gtcttttacg ccaagcgata gcccagtttt
    12961 ttccagaatt tgttctttcg aaagtccttg ttcgatatat ttgaacgctc gctgtggatg
    13021 ctcgccaaat attttataaa gatttttaat ttctaattta attgccatgc aatagaaaga
    13081 ttcctttatt tgtttatgtc gatatattac ccaatggaaa tagtcacttt tttctaccct
    13141 aacatactga gaatctgagg caacccctga gggcaaatgt ggcatgaata ttgatgcgag
    13201 caaatcgccg gaatttcccg tgatataagg gctgagagca aatcgagaaa agttgatatt
    13261 ttttgtcata gatagcgaaa aaacgcctga atattgtagt ttcaggcgaa taatgcaaca
    13321 gagtctcagt gtagatcact acagaatatt tgcgtgataa aaagctattt ggcgggaatt
    13381 atttccctgc tgcgggtagt gatatttttg aaaataacac cctcagaaat tccagtcttc
    13441 atcttctgtt tcaaccgctt tccccatcac ataagaggag cctgaaccgg aaaagaaatc
    13501 gtgattttca tcggcattcg gcgaaagcgc ggcgaggatt gccggattca cttccgccat
    13561 ttctgcggga aataacggtt cgtagcccag attcatcaaa gccttattgg cgttgtaaca
    13621 gagaaacgct ttcacatcgt cagcccacgg ggtttcggcg tacagctcat cggtgtattg
    13681 caactcgttg tcgtagagtt ccagcaacaa atcgaaggcg aaattcttca actcttcacg
    13741 ttgtcccaga gatatctttt ccatgttttt ctgatattta tagcctatgt agtaaccgtg
    13801 gactgcttca tcgcggataa tcagacggat cagatctgcg gtattggtca gctttccgcg
    13861 gctggaaaaa tacatcggca gccagaaacc ggaatagaac aaaaaagatt caagaaacac
    13921 actggcgatt ttctttttca gcggatcatc accgcgataa tgttgctgaa taatctgagc
    13981 ttttcgctgc aacggtgcgt tttcttcact ccaggcgtag gcggcatcga catctttggt
    14041 ctgacacagc gtcgagaaaa tcgaactgta agagcgggca tgaaccgctt ccataaagct
    14101 gatattcgat aataccgctt cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc
    14161 gccgataaca ttttgcagcg tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag
    14221 ttgttgttct acgacagtta atgtctgcca ggcaggaata tcgttcgaca gcggcacctt
    14281 ttctggtagc cagaaattgc tggtcaggcg attccacacc tccagatctt tatcgtcaga
    14341 tatcttgttc cagttgatgg cgctgatacg tgagagtttc atagatattc cttaaagtgc
    14401 acaggagacg cagccttcaa tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta
    14461 atagagcgtt ttgataccct tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt
    14521 ggtggcggta tcggggaaaa acagcgtcag cgacagcccc tgatcgacat ggcgagtcgc
    14581 ttcggcgtag gtatcgatga tcttttctgc gccgatttcg taagcatcct gatacagcgc
    14641 cagattctcg ttagtcataa acggggcagg gtagtaaacg cgtcctgttt tgccctcttt
    14701 gcgtatctct actttcgcca caatcggatg aatactcgac gtagcatggt tgatataaga
    14761 gatagaaccg gttggcggca ccgcctgaag attctggtta taaatgccgt agcgcatcac
    14821 gtcgtcgcgc agttgcgccc acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa
    14881 cagttcgcca actttcgccg ttttcggctg ccagttacct tgcagatatt ggctaaaata
    14941 ttcaccactg gcataacgtg actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc
    15001 cagcaacatc gaggtacgca gtgcgtgcca ggtgatggca tagaaataga gattggtgaa
    15061 atccagtgct tccggcgaac cataagcgat gccttctcgc gccagatagc cgtgtaaatt
    15121 catctgcccc agtccgatgg cgtgtgaggc ggcatttcca gcttcgatgg acggcacgct
    15181 gcggatatga ctcatatctg acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg
    15241 ggcaaaatcg ggggaatcca tggtgtgcgc aatattcaac gaacctaaat tgcaggagat
    15301 atcatggcct gtgcgggcat agtcgagatt ctcgtcatac tctgaggcac agttgacctg
    15361 caaaatctct gagcagagat tactcatatt tatgcgcccg gcgacagggt tagcacggtt
    15421 taccgtgtct tcatacatga tgtagggata gccggactca aactggattt ctgccagccg
    15481 ctggaagaaa tcacgggcgt tgaggtattt tttgcggatg cgttcatcgg caaccagttc
    15541 gtcatagtgt tcgctgatgg cgatatcggc aaacggcttg ccataaactc gctcaacgtc
    15601 atagggcgaa aacagcgcca tctgcgcatt ctcttttgcc agatggaaag tgatatccgg
    15661 gatcaccacg cccagcgaca gtgttttaat gcggattttt tcgtcggcat tttcccgttt
    15721 tgtatcgaga aaacgcagaa tatcgggatg atgagcatgt aaatagactg cacctgcccc
    15781 ctgacgcgcg ccgagttggt tggcatagga aaatgcgtct tccagcattt tcatcaccgg
    15841 aattacgcca gaagattgat tttcaatacg tttaattggc gcgcccgctt ctcgcagatt
    15901 cgacagcaaa aatgccacgc cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc
    15961 ccggccaatc gactccatat tgtcttcaat acgcagcaga aaacaggaaa ccagttcgcc
    16021 gcgctgctgc ttaccgcagt tgaggaatgt cggcgtggct ggctgaaagc gtcctgacag
    16081 catttcatcg gtcagttgca acgccagcgt cgcatcgcct tgtgccagcg tcagcgccac
    16141 catcgtgact cgatcggcaa aatcttccag ataacgttta ccgtcgaatg tcttcaacgt
    16201 atagctggtg taaaacttcc atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg
    16261 tgcgtgggca aacagcgtaa tgacaaagtc gcgagagtag cgattaagaa cgctttcatc
    16321 atagtaacct tcgttgacca gccagttcag gcgctgctgc tggctactga aggtcacact
    16381 gttcggacgc acatgcgtcg ccataaaggc gtcaacggcc tggcggtctt tatcgaactg
    16441 aatgcgacct gcgctatcgt agaggttaag catcgcattc agcgcgtggt aatccatcgt
    16501 ttcctgcgtc aggcattctg cggttgtcgt tgccaaaatt cggttactcc tttacgaacg
    16561 ttttcgatat cgctttgcgt acccatgagt tcaaaacggt acagccacgg cacgccgcat
    16621 ttccgggcaa tcacatctcc ggcgcggcca tacgcctcac taaagttgcg attaccagaa
    16681 gcaataacgc cgcgaagcaa cgcccggttg tgctcgtcgt ttaaaaagcg aattacctgt
    16741 cgtggcaccg cgccagccgt accgccgccg ccgtaagagg gcacgatcag gatgtaaggc
    16801 tcgtctacct gaatccgttc ccgctcattg agcgggatgc gcaccgcggg cagacctaaa
    16861 cgttcgataa aacgctgcgt gttttcggag ctgctggaga agtagacgag ctggctcatg
    16921 cactggccgc gtgtggcgct ggatgcagac ggttaatcat gtccggacgg aaaccagacc
    16981 agctaagatc gccagcaatc actaccggca actgacgaaa gccctgagca cgcaacgctt
    17041 ccgctgcttc aggaacgcga tcgacattaa tcatttcaaa atcaaacccc cggttttcca
    17101 tcgcccgttt ggtggcgtgg cactgaacgc aatcgttacg agtgtaaata gttatgcgca
    17161 tgattcgtat ttccgtttaa aatgaagata cggcgcgatg atacgcgtcg ggttgtctct
    17221 ctgttgatac agagatacta gatgtagttg aaaaaagatt caactacaca atatatagca
    17281 aatagtgatc gaaattaccc tggatatgag cgtgacgggg gagggggatt tttgtgattc
    17341 accaggcaaa aagaaacccc gaagacaggc ttcggggtca aagacgcgta tttattatca
    17401 tttttgcact acgatttgcg catgcttaac agtgcgccga taaaaatacc taccgcagct
    17461 gctgtaccca cgctacacca gggtctttca cgaacaaaag aatccgcgca gccaacggca
    17521 tcgcgcgcgg cttgctggac gcgagtacga ccatgcattc gcgccctggt ttctttcatc
    17581 aacgcctgag ctttactgcg tgcggcttca gcttcccctt tggcgtcgct gccccaggac
    17641 ttcaatacag attcgaggct gtcagctaat tgattgacat cattctggat atcctgcacg
    17701 ccatcatcaa cgtcgttgcg gttcggtcgg ttaaacatat gatcacccat tagaattcga
    17761 tgtaattttc agtgtagacc ataattttct taactgaggt tcatcgcggc tttatggact
    17821 tttctgaaag cgttgtgaat gcgcaatcag gtaaggtagg cccgcgg
//
LOCUS       DAJTMK010000082        17487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTMK010000082 DAJTMK010000000
VERSION     DAJTMK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17487
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.083"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(36..2633)
                     /gene="topA"
                     /locus_tag="OO135_002315"
     CDS             complement(36..2633)
                     /gene="topA"
                     /locus_tag="OO135_002315"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5398142.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3013..3264
                     /locus_tag="OO135_002316"
     CDS             3013..3264
                     /locus_tag="OO135_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5398143.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3300..4349)
                     /gene="sohB"
                     /locus_tag="OO135_002317"
     CDS             complement(3300..4349)
                     /gene="sohB"
                     /locus_tag="OO135_002317"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5398144.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4569..5327
                     /locus_tag="OO135_002318"
     CDS             4569..5327
                     /locus_tag="OO135_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5398145.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEEPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5324..5915
                     /gene="cobO"
                     /locus_tag="OO135_002319"
                     /pseudo
     CDS             5324..5915
                     /gene="cobO"
                     /locus_tag="OO135_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5955..6827)
                     /gene="rluB"
                     /locus_tag="OO135_002320"
     CDS             complement(5955..6827)
                     /gene="rluB"
                     /locus_tag="OO135_002320"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5398146.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7038..8933)
                     /locus_tag="OO135_002321"
     CDS             complement(7038..8933)
                     /locus_tag="OO135_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR5398147.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKYPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINIGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8961..9581)
                     /locus_tag="OO135_002322"
     CDS             complement(8961..9581)
                     /locus_tag="OO135_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5398148.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9578..10459)
                     /gene="yciV"
                     /locus_tag="OO135_002323"
     CDS             complement(9578..10459)
                     /gene="yciV"
                     /locus_tag="OO135_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5398149.1"
                     /translation="MSDTNYAVIYDLHSHTIASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10597..10641
                     /gene="trpL"
                     /locus_tag="OO135_002324"
     CDS             10597..10641
                     /gene="trpL"
                     /locus_tag="OO135_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5398150.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10618..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10733..12295
                     /gene="trpE"
                     /locus_tag="OO135_002325"
     CDS             10733..12295
                     /gene="trpE"
                     /locus_tag="OO135_002325"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5398151.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSMPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVANLTKVDR
                     YSYVMYQVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12295..13890
                     /gene="trpD"
                     /locus_tag="OO135_002326"
     CDS             12295..13890
                     /gene="trpD"
                     /locus_tag="OO135_002326"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5398152.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13891..15252
                     /gene="trpCF"
                     /locus_tag="OO135_002327"
     CDS             13891..15252
                     /gene="trpCF"
                     /locus_tag="OO135_002327"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5398153.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQCFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15264..16457
                     /gene="trpB"
                     /locus_tag="OO135_002328"
     CDS             15264..16457
                     /gene="trpB"
                     /locus_tag="OO135_002328"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5398154.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMTQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     SRGEI"
     gene            16457..17263
                     /gene="trpA"
                     /locus_tag="OO135_002329"
     CDS             16457..17263
                     /gene="trpA"
                     /locus_tag="OO135_002329"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5398155.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQSEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4222 a         4669 c         4345 g         4251 t
ORIGIN      
        1 accctttgtt tataaaaacc tgacagaatt aaaggttatt tttttccttc aacccatttg
       61 ccatcaacat aaaatgctga ccagccagtc gcctttccgt ctttttccga agagacatat
      121 tgctgtttgg ttttacggct aaagcgaacc atggtcttat taccttccgg atcctgctgt
      181 ggcgcatcgg ccagataacg cagtttttcc ggcagacggt cgcggaagcg ataaagctct
      241 tccaccagtg gcgcacgcgt ttcacgcgat ttcgggaaag tgttggcagc caggaaaaca
      301 cccgcagcgc cgtcacgcag cacgaaataa gcatctgatt tttcgcacgg cagctcaggt
      361 aatggcaccg gatcttcttt cggtggtgcc acttcgccgt tacgtaaaat cttacgtgtg
      421 tttttacact cttcgttggt gcaggccatg tatttaccga aacgccccat tttcaggtgc
      481 atttcagagc cacatttttc acactcaacg atcgggccgt cataaccttt aatgcggaat
      541 tcgccctctt cgatctcgta accgtcgcag gttgggttat taccacagac atgcaactta
      601 cgtttcggat cgatgagata gctgtccatc gccgtgccgc atttcgggca acgacgtttt
      661 gcgcgcagcg cgttggtttc agcgtcttcg ccttccagta cgttcagcac ttcgttttcc
      721 ggcaccaggt taatggtggt tttgcaacgc tctttcggcg gcagcgcata accagaacag
      781 ccaaggaata ccccggtgct cgctgtgcga atccccattt tgcgaccaca agtcgggcag
      841 tcaatgctgg tcagaaccat ctggttcggg cgcataccac cctcttccgg atctttttca
      901 gctttatcta actgctgggt gaaatccgag aagaagtgat ccagtacagc tttccactct
      961 gcttcgtgat ttgccacctg gtcgaggctg ttttccatct gtgcggtgaa gtcgtagttc
     1021 attaactcgc ggaaattttc ttcaaggcga tcggtgacga tttcgcccat tttttccgca
     1081 tagaaacgac gattttctac tcgcacgtag ccacgatcct gaatggtcga aatgatcgac
     1141 gcataggtag atggacgacc gataccgcgt ttttccagct ctttaaccag cgatgcttca
     1201 ctgaaacggg ctggcggctt ggtaaagtgc tgggctggtg taagttcaac gagcgtcaga
     1261 gcatcgcctt tattaactgc tggtaagatg cgatcttcat cgcctttacg caacgcaggc
     1321 atcacttttg tccagccatc aaaacgcaaa atacgaccgc gtgctttcag gcggaaatcg
     1381 cccgcaccaa cggtcagcgt cgtggagtca tatttcgctg gggtcatctg gcaggctacg
     1441 aactgacgcc agattaactg gtacagtttc tgcgcatctg cttccatatc cttcagcgat
     1501 tccgccatca cattgacgtc agaaggacga atcgcttcgt gcgcttcctg tgagttttct
     1561 ttgctggcgt actgattcgg actttccggc agatatttct taccaaaatt atcgctgata
     1621 taaccgcgaa ccatatttac cgcgtcctga ctcaggttag tggagtcggt acgcatgtaa
     1681 gtgatatagc ctgcttcata caaacgctgc gccatcatca tggttttttt cacgccaaat
     1741 ccaagacggg tgctggcagc ttgttgcagc gtagaggtaa taaaaggagc gccaggttta
     1801 ctggttgtcg gtttgtcttc acgttccagc acgctgtagc gcgctttttc cagcagactt
     1861 accgcagcct gagtttgttc tttgttgacc ggacggaacg gtttgtcgtt ctgatgagtc
     1921 acctgtaacg ccaacgcttc accagatggc gtggtcgtgc tggcatcgac ttcccagaac
     1981 tcttccggca cgaacgcttt aatttcacgc tcacgctcga ccaccaggcg aaccgccacc
     2041 gactgcacac gaccggcaga cagaccacga gcgatctttt tccatagcag cggcgaaacc
     2101 atatacccca ccacgcggtc cataaagcga cgcgcctgct gggcattaac acgatcaata
     2161 ttcagctcac ccggtttgtt aaatgcctgg cggatcgcgt ttttagtaat ttcgttaaac
     2221 accactcggc tatagcgcgc atcatcaccc ccaatcactt cccgcaggtg ccatgcaatg
     2281 gcttcccctt cgcggtcaag gtcggttgcg agatagatgt ggtcggcttt ttcagccagt
     2341 tgtttcagtt cagagacgac cttctcttta ccaggcaaca cttcatagtg cgcctcccaa
     2401 ttgtgccacg ggtcaacccc catacggttg acgagagcgc cacgttcatc ctttttaggc
     2461 tttttagccg tcttggtgga ggtagagtcg gcactctttt tggcagctga gccactggtc
     2521 ggcaaatcgc ggatgtgacc gacgctggat ttcaccacgt agtcactacc cagatacttg
     2581 ttgatcgttt tggcttttgc cggggactca acgatgacaa gagctttacc catattcacc
     2641 ttacctaatt tgattcttcc aggaatgcgt cgcacgttga taaaccttcc actggcaacg
     2701 agttaccgat atggactcgt tctctgcgga tatcaacccc ttgtcgtgac tctgttcacg
     2761 aaaattcagg tgactgagtt aacaggtctt ggcctacagg cgctatagca cgacggaatt
     2821 ttggtcgaat gtcaagcaaa tctattgcca gattgacgaa agcgtcacac tgtacctgat
     2881 aaaatccgat acgcaacttt attagcatgc aaaatccaaa gttgccagta aggcgctctt
     2941 gccaccagcg tggtatattt gtctgcaagc catgcctggc gtagactaat gtccttgttt
     3001 aaggagtaaa taatgaacaa agagacacaa cccatcgacc gcgaaacgct gttgaaagag
     3061 gccaacaaaa tcattcgtga gcatgaggat acgctcgcag ggattgaagc caccggcgtc
     3121 acgcagcgta atggcgtgct ggtatttacc ggcgactact ttttagatga acaaggattg
     3181 ccaacagcca aaagcaccgc ggtgtttaat atgttcaaac acctggccca tgtgctttca
     3241 gaaaaatatc atctggttga ttaaagcaaa acgcgaggtc ttagcctcgc gtttgtcttt
     3301 tacatcaatg gcttttgacc ccgctgccac cagcgtagca acaatcgatc ggcgctctct
     3361 gccgcgctgc cggtgaatcg gtcaatgagt cgtttacgct gcatatagcg tacattaacc
     3421 acttcacggc cttccatcag gctaagaata acttcatcac tggtgttgat ttcatcaacc
     3481 aggcctttct ctaccgcctg ttgtccgtac cagtgttcac ctgttgccac ctgttcaata
     3541 tccagagacg gacgcatacg cttcacaaaa tctttaaaca actgatgcgt ttcgttcaac
     3601 tcttcgcgga atttctcccg cccttcttcg gtattttcac ccagcaacgt cagagtacgc
     3661 ttatactgcc cggcagtgtg cagttcgata tcaatatatt tgcttttcag gaagcggtta
     3721 aagttgggca tttgcgccac caccccaatg gaacccacaa tagcaaacgg tgcggaaaca
     3781 attttgtccg ccacacaggc catcatgtaa ccgccgctgg cagcgacttt gtctaccgta
     3841 acagttaaag gaatgttttt atcacgcaga cgctgcagct gcgaagccgc caacccgtaa
     3901 ccatgcacca tgccaccagg gctttccaga cggagcacaa cctgatcctg cggtttgaat
     3961 gctgcgagta ccgccgttat ctcttcacgt agcgagttca cttcatgggc gtccatgctg
     4021 cctttaaaat ccagcaccca gacgcgaggt ttactgtcag ttgccacctc gcccagtttg
     4081 gctttcgctt ttgctgcttt cgcttcttgc ttgtgctttt tcttctgcgc tttgtgccac
     4141 tgtttttgct gatgtgagcc catcagcgcc gcggccagtt cttctttcat ctccttatac
     4201 tgttcgctga gattgttgac ccgtaactcg ccacgctggc gtttattacg ttgagcaaca
     4261 ttgacaataa tggcggcaat cgccgcaatc gctagcacaa cggtaacgat tttcgccaaa
     4321 aacaaaccat attcagacaa caattccacg agtccccacc ttggttaaac aacgctttgg
     4381 ctcacagttt acaacaggca tgttaagccg tctcgcagag atgttattgc agcgaaagta
     4441 tgacaaagta tccattttgc ggtgttatcg ccccaggctg attgaaatgc ccttctgttt
     4501 caggcataaa gccccaaagt cataaagtac actggcagcg cgacagacaa cgtcgttgag
     4561 gagtagccat gcattaccag ccaaaacaag atttactcaa tgatcgcatt atcctggtga
     4621 cgggagccag cgatgggatt ggtcgtgaag ccgcgatgac gtatgcacgc tatggtgcga
     4681 cagtgattct gttgggccgt aatgaagaaa aattacgtca ggtagccagc cacataaacg
     4741 aagaaactgg gcgtcagcca cagtggttta ttctcgattt gctgacctgc acgtccgaaa
     4801 attgccaaca actggcacag cgcattgtcg ttaattatcc gcgtctggat ggtgttttgc
     4861 ataatgccgg attgctcggc gatatttgcc caatgagcga acaaaatccg caggtctggc
     4921 aggacgtcat gcaggtcaac gttaatgcca cctttatgct cacccaggca ctgcttcctt
     4981 tattactcaa atcggacgcc ggttcactgg tctttacttc atcaagcgtt ggacgtcagg
     5041 gacgagctaa ctggggtgca tatgcagcgt cgaaatttgc caccgaaggg atgatgcagg
     5101 tactggccga tgaatatcag cagcgcctgc gcgtcaactg cattaaccca ggcggtactc
     5161 gcaccgcaat gcgcgcaagc gccttcccga ccgaagagcc acagaaactt aaaacacccg
     5221 ctgatatcat gccgctctac ctctggctga tgggcgatga cagccgccgt aaaaccggca
     5281 tgacctttga cgcccaaccg ggccgtaaac caggaatttc ccaatgagtg atgaacgcta
     5341 ccaacagcgt cagcagcgag tgaaagaaaa agtagatgct cgtgtggccc aggccccagg
     5401 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
     5461 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
     5521 aaggcacctg gcctaatggc gaacgcaatc tgctggaacc acatggcgtt gagtttcagg
     5581 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
     5641 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
     5701 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
     5761 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
     5821 atattcttga actggcggat acggtaagtg agctgcgccc catcaaacat gcgtttgatg
     5881 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
     5941 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgaccgcc gctcacctga
     6001 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
     6061 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
     6121 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
     6181 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
     6241 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
     6301 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
     6361 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
     6421 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
     6481 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
     6541 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
     6601 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
     6661 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
     6721 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
     6781 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
     6841 ggtgtcgcct tcccaggcgt ctgatatgct tttgtgcaac agtgagtcag aacttacatc
     6901 tgactcactg atagtaaagt ctgtttgaac tcttcaggcc ttaagtaaac gactcgacgt
     6961 ttatcggcct ggctttgagg cgcaacatac tacactatct ttatggttaa agcttgtttc
     7021 gattaacatc agcttaatta ccagccacca ccgcctccgc cacccgcgcc gccgccagca
     7081 ccaccaccgg aataacttcg ccccaacaaa ctggtattgg ccccgcaact taggtgcgtc
     7141 tgtaatgaga ataaaacgcc gttacgggca atctctggag cgcgaatcgc cgaagaaagc
     7201 gaaggggcaa tgcgtaacgc ccattccctc cccaaatttg ccgatatagc ccaacctagc
     7261 aacgcgtaat ccagtttccg tgtttcccct ttccgccgac gtccactgtg tgttgctgcc
     7321 tctttacgcg caagatagtt aacgatagct tcaccgcggg cataacgttg ctgcccagtt
     7381 tgggtaaact ttggcagaaa aatatacccc atgccagtga gataaccggt caggcaaaca
     7441 gcgctaaaat aacccgctgg catataccag taaaagaata catctccgat attgatatag
     7501 agagaataga aggccacgcc tgaacaaatc agtggtaaaa acaaagtgat gattgcacga
     7561 ttaacgcaag gcttgtcatc atcgcgggca aacaaaagct caagggaaca aaataaaaaa
     7621 ggaataatcc agcagatcat aataaacata tcgcctacca gactcattcc tgcccaacct
     7681 acgggactca ggttaccgta gagaatgatc gccaatattg ccagtccacc ccattgcaaa
     7741 aacgtatcag gccggtaaaa caaagacttt ttacgcggca gataaaattt ctgcatccgt
     7801 aaaaatgccc tgcgtaagac tctgttgcgt ctccccgtaa gaacgacctt atcgccttta
     7861 cgaaataacg cttccagtaa taactgctct gcgggcagta atacctcatt atttctggtc
     7921 gcgccttcat taacacaaat caggatttgc tggttcttat catactgatc ttccagatga
     7981 atttttccct tcacgatcag atttacgata tcggcacaga aacctttatc gtcgtaaacc
     8041 agcttcgcat cgagacgtaa catgccgggc gtgtaatctg gcggaatgac atcggtttca
     8101 atcacatcta ccggcgtaaa ttgcggcctg cgcttccaga gatataacca tccacaaacc
     8161 aggaatagac acggaaacca aattaacaac gatgacgtgg agggtaaaag aagatggctg
     8221 aaaatattcg tcgtttgtgg agccggggca ttgctaagta acgcgtgagg ccagcggtag
     8281 agtaccgtga aatcttcacg ataaaacgga tcgcgggatt caattcttcc gtcctcaagg
     8341 atgcggccat ttcgatatgt gtcgccctct tctccagtaa agagatcgat ttcgctaaat
     8401 ggattacccg cgatatcagg gtacttgagt gaaaatcggg tcttatagat ttcaaacggc
     8461 cagtggttac cagtcacgtt ccagattaac agatctgaat ctccctcacg caggaaagca
     8521 tttttaacct gataatgaat ttgataatga tagtcgcctt gaggcaagaa acgttgttta
     8581 tcgcccacga caatactcat taacccgata tgccagtcaa gatgccaggg ttcaggaata
     8641 ccatcacgag tgacgccaac aatatgatag ttcagcagtg cagcgtcccc atcaggctgc
     8701 atccagagtc gtggtaaatc acgataaaat ccgtgcttaa tctgctgatt tcgcgcctga
     8761 aaatgaatat tttctttgat gtcgagcacc ccatccggtt gcagagtggc ttcaacatca
     8821 aagagcgaga tcccttcttc ataagaaggg atctccgggt tttctgcata acttagagat
     8881 gatacaaaca gccctgctat caacagcaaa atgcagcgaa acttccctgc catagtcttc
     8941 catccgtgga acgcaatttg ttataagaaa ggcttcacat cacccacgcc ttcacgcacc
     9001 acgaccggcg tatcatcggt aagatcaatg accgttgtcg gtttctgtcc gagatagccg
     9061 ccgtggataa tcaaatccac ctgtttttcc agacggtctt taatttcttc tggatcggat
     9121 tcggtaaatt cgctgcctgg cagcattagc gaagtggaaa gcatcggttc gcccagtgct
     9181 tcaagcaacg cctgcgcaat cgggttagaa ggcacacgca taccgatggt tttgcgtttt
     9241 tcctgcaaca gacggcgtgg cacctccttc gtccccttca ggatgaaggt atagttgccc
     9301 ggcgtgttgt ttttcattaa acgaaacgca acgttatcca cgaatgaata ggtcgaaagt
     9361 tcagaaagat cgcgacacat cagggtaaag ttgtgaccgt ccggcagctg gcgaatacga
     9421 caaatacgct ccatcgcgtt tttatcttca attttacagc cgagcgcata gccggaatca
     9481 gttggataaa caatcacccc gcctttacgc acgatctcca ccgcctggtt gatcagacgt
     9541 tgctgcgggt tatcaggatg aatataaaaa aactggctca taattccctc tctgtggtgt
     9601 tctgcggctg ttcccatagc tgccagacgc cttcaacgcc tgcgggcagc cagagtttac
     9661 gacccagttc gatccacggg catggctgat gaaaatcaga tccttgtgat gcccataaat
     9721 gatgctgacg cgcaagtgtc gtcaactggg tgcgttcatt gggcgattgc tggcactgcg
     9781 cgacttccat cgcgtcgccg tggtgttcgg caaaatgcgc aaccaatctt ttcagccatt
     9841 tagcactaag attgtaccgc ccaggatgag ctagcaccgc cttaccgcca gaatgatgaa
     9901 tgacatcaat agcttgttct attgtacacc actgtggcgg aacgtatccg gttttcccgc
     9961 gcgccagata ctttttaaag acatctgcca ttgaactggc tttgccgcac tcaacaagga
    10021 aacgcgcaaa atgaccgcgc gtcactgcgc cgccctgcgc cagtcgttgt gcgccttcca
    10081 gcgccccagg tatttgcgct ttttcaagtc tttcggcaat ttgctgcgcc cgctgattgc
    10141 gccgttctgt ctgctgcgcg aggaactcac acattagcgg atgagtaata tcaatattca
    10201 gcccgacaat atgaatttca tgattttccc agaccgtgga aatttccacg ccgggaataa
    10261 gattcagcgc cagtcccgaa cgtgaaattt cctctcttgc tggcgcgatt gcagctgtgg
    10321 tgtcatggtc ggtgatcgcc agggtgccga cgcgcatctc gactgcacgg tgcaccaatg
    10381 cttctggcgt caggcagcca tcggaagcta tggtatggct gtgcaggtcg taaatcactg
    10441 cgtaattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc
    10501 ataacggttc tggcaaatat tctgaaatca gctgttgaca attaatcatc gaactagtta
    10561 actagtacgc tagttcacgt aaaaagggta tcgacaatga aagcaatttt cgtactgaaa
    10621 gcttggtggc gcacttcctg aaacgggcag tgtattcacc atgcgtaaag caatcagata
    10681 cccagcccgc ctaatgagcg ggcttttttt tgaacaaaat tagagaataa caatgcaaac
    10741 acaaaaaccg actctcgaac tgctaacctg cgaaggcgct tatcgcgaca acccgactgc
    10801 gctttttcac cagttgtgtg gggatcgtcc ggcaacgctg ctgctggaat ccgcagatat
    10861 cgacagcaaa gatgatttaa aaagcctgct actggtagac agtgcgctgc gcattacagc
    10921 attaggtgac actgtcacaa ttcaggcact ttccggcaac ggcgaagccc tgctgacact
    10981 actggataac gccctgcctg cgggtgtgga aaatgaacaa tcaccaaact gccgtgtgct
    11041 gcgcttcccc cctgtcagtc cactgctgga tgaagacgct cgcttatgct ccctttcggt
    11101 ttttgacgct ttccgtttat tgcagaatct gttgaatgta ccgaaggaag aacgagaagc
    11161 aatgttcttc ggcggcctgt tctcttatga ccttgtggcg ggatttgaag atttaccgca
    11221 actgtcagcg gaaaataact gccctgattt ctgtttttat ctcgctgaaa cgctgatggt
    11281 gattgaccat cagaaaaaaa acacccgcat tcaggccagc ctgtttgctc cgaatgaaga
    11341 agaaaaacaa cgtctcactg ctcgcctgaa cgatcttcgc cagcagctga ccgaagccgc
    11401 gccgccgctg ccggtggttt ccatgccgca tatgcgttgt gaatgtaacc agagcgatga
    11461 agagttcggt ggcgtggtgc gtttgttgca aaaagcgatt cgcaccggtg aaattttcca
    11521 ggtggtgccg tctcgccgtt tctctctgcc ctgcccgtca ccgctggcgg cctattacgt
    11581 gctgaaaaag agtaatccca gcccgtacat gttttttatg caggataatg atttcaccct
    11641 gtttggcgcg tcgccggaaa gttcgctcaa gtatgacgcc accagccgcc agattgagat
    11701 ctacccgatt gccggaacac gcccgcgcgg tcgtcgcgcc gatggttcac tggacagaga
    11761 cctcgacagc cgcatcgaac tggaaatgcg taccgatcat aaagagcttt ctgaacatct
    11821 gatgctggtg gatctcgccc gtaatgatct ggcacgcatt tgcacccccg gcagccgcta
    11881 cgtcgccaat cttaccaaag ttgaccgtta ctcttacgtg atgtaccagg tctcccgcgt
    11941 ggtcggtgag ctgcgccacg atctcgacgc cctgcacgct taccgcgcct gtatgaatat
    12001 ggggacgtta agcggtgcgc cgaaagtacg cgccatgcag ttaattgccg aggcggaagg
    12061 tcgtcgccgc ggcagctacg gcggcgcggt aggttatttc accgcgcatg gcgatctcga
    12121 cacctgcatt gtgatccgct cggcgctggt ggaaaacggt atcgccaccg tgcaagccgg
    12181 tgctggcata gtccttgatt ctgttccgca gtcggaagcc gacgaaaccc gtaataaagc
    12241 ccgcgctgta ctgcgcgcta ttgccaccgc gcatcatgca caggagactt tctgatggct
    12301 gacattctgc tgctcgataa tatcgactct tttacgtaca acctggcaga tcagttgcgc
    12361 agcaatgggc ataatgtggt gatttaccgc aaccatattc cggcgcagac cttaattgaa
    12421 cgcctggcga cgatgagcaa tccggtgctg atgctttctc ctggccccgg tgtgccgagc
    12481 gaagccggtt gtatgccgga actcctcacc cgcttgcgtg gcaagctgcc cattattggc
    12541 atttgcctcg gacatcaggc gattgtcgaa gcttacgggg gctatgtcgg tcaggcgggc
    12601 gaaattctcc acggtaaagc ctccagcatt gaacacgacg gtcaggcgat gtttgccgga
    12661 ttaacaaacc cgctgccagt ggcgcgttat cactcgctgg ttggcagtaa cattccggcc
    12721 ggtttaacca tcaacgccca ttttaatggc atggtgatgg cagtacgtca cgatgcggat
    12781 cgcgtttgtg gattccagtt ccatccggaa tccattctta ctacccaggg cgctcgcctg
    12841 ctggaacaaa cgctggcctg ggcgcagcgg aaactagagc caaccaacac gctgcaaccg
    12901 attctggaaa aactgtatca ggcgcagacg cttagccagc aagaaagcca ccagctgttt
    12961 tcagcagtgg tacgtggcga actgaaaccg gaacaactgg cggcggcgct ggtgagcatg
    13021 aaaattcgcg gcgagcaccc gaacgagatc gccggagcag caaccgcgtt actggaaaac
    13081 gccgcgccgt tcccgcgccc ggattatctg tttgccgata tcgtcggcac cggtggtgac
    13141 ggcagtaaca gcatcaatat ttccaccgcc agtgcgtttg tcgccgcggc ctgtgggctg
    13201 aaagtggcga aacacggcaa ccgtagcgtc tccagtaaat ctggttcgtc cgatctgctg
    13261 gcggcgttcg gtattaatct tgatatgaat gccgataaat cgcgccaagc gctggatgag
    13321 ttaggcgtat gtttcctctt tgcgccgaaa tatcacaccg gatttcgcca tgcgatgccg
    13381 gttcgccagc aactgaaaac ccgcactctg ttcaacgtgc tgggaccatt gattaacccg
    13441 gcgcatccgc cgctggcgtt aattggtgtt tatagtccgg aactggtgct gccgattgcc
    13501 gaaaccttgc gcgtgctggg gtatcaacgc gcggcggtag tgcacagcgg cggtatggat
    13561 gaagtttcat tacacgcgcc gacaatcgtt gccgaactgc atgacggtga aattaagagc
    13621 tatcagctaa ctgcagaaga ttttggcctg acaccctacc accaggagca actggcaggc
    13681 ggaacaccgg aagaaaaccg tgacatttta acacgcttgt tacaaggtaa aggcgacgcc
    13741 gctcacgaag cagccgtcgc ggcgaatgtc gccatgttaa tgcgcctgca tggccatgaa
    13801 gatctgcaag ccaatgcgca aaccgttctt gaggtactgc gcagtggttc cgcttacgac
    13861 agagttaccg cactggcggc acgagggtaa atgatgcaaa ccgttttagc gaaaatcgtc
    13921 gcagacaagg cgatttgggt agaaacccgc aaacagcagc aaccgctggc cagttttcag
    13981 aatgaggttc agccgagcac gcgacatttt tatgatgcgc tacagggtgc acgcacggcg
    14041 tttattctgg agtgcaagaa agcgtcgccg tcaaaaggcg tgatccgtga tgatttcgat
    14101 ccggcacgca ttgccgccat ttataaacat tacgcttcgg caatttcggt gctgactgat
    14161 gagaaatatt ttcaggggag ctttgatttc ctccccatcg tcagccaaat cgccccgcag
    14221 ccgattttat gtaaagactt cattatcgac ccttaccaga tctatctggc gcgctattac
    14281 caggccgatg cctgcttatt aatgctttca gtactggatg acgaccaata tcgccagctt
    14341 gcagccgtcg ctcacagtct ggagatgggg gggctgaccg aagtcagtaa tgaagaggaa
    14401 ctggagcgcg ccattgcatt aggggcaaag gtcgttggca tcaacaaccg cgatctacgt
    14461 gatttgtcga ttgatctcaa ccgtacccgc gagcttgcgc cgaaactggg gcacaacgta
    14521 acggtaatca gcgaatccgg catcaatact tacgctcagg tgcgcgagtt aagccacttc
    14581 gctaacggtt ttctgattgg ttcggcgttg atggcccatg acgatttgca cgccgccgtg
    14641 cgccgggtgt tgctgggtga gaataaagta tgtggcctga cgcgtgggca agatgctaaa
    14701 gcagcttatg acgcgggcgc gatttacggt gggttgattt ttgtcgcgac atcaccgcgt
    14761 tgcgtcaacg ttgaacaggc gcaggaagtg atggctgcgg caccgttgca gtatgttggc
    14821 gtgttccgca atcacgatat tgccgatgtg gtggacaaag ctaaggtgtt atcgctggcg
    14881 gcagtgcaac tgcatggtaa tgaagatcag ctgtatatcg atacgctgcg tgaagctctg
    14941 ccagcacatg ttgccatctg gaaagcatta agcgtcggtg aaaccctgcc cgcccgcgag
    15001 tttcagcacg ttgataaata tgttttagac aacggccagg gtggaagcgg gcaatgtttt
    15061 gactggtcac tattaaatgg tcaatcgctt ggcaacgttc tgctggcggg gggcttaggc
    15121 gcagataact gcgtggaagc ggcacaaacc ggctgcgccg gacttgattt taattctgct
    15181 gtagagtcgc aaccgggcat caaagacgca cgtcttttgg cctcggtttt ccagacgctg
    15241 cgcgcatatt aaggaaagga acaatgacaa cattacttaa cccctatttt ggtgagtttg
    15301 gcggcatgta cgtgccacaa atcctgatgc ctgctctgcg ccagctggaa gaagcttttg
    15361 tcagcgcgca aaaagatcct gaatttcagg ctcagttcaa cgacctgctg aaaaactatg
    15421 ccgggcgtcc aaccgcgctg accaaatgcc agaacattac ggctgggacg aacaccacgc
    15481 tgtatctgaa gcgcgaagat ttgctgcacg gcggcgcgca taaaactaac caggtgctcg
    15541 gtcaggcttt actggcgaag cggatgggta aaaccgaaat catcgccgaa accggtgccg
    15601 gtcagcatgg cgtggcgtcg gcccttgcca gcgccctgct cggcctgaaa tgccgtattt
    15661 atatgggtgc caaagacgtt gaacgccagt cgcctaacgt ttttcgtatg cgattaatgg
    15721 gtgcggaagt gatcccggta catagcggtt ccgcgacgct gaaagatgcc tgtaacgagg
    15781 cgctgcgcga ctggtccggc agttatgaaa ccgcgcacta tatgctgggc accgcagctg
    15841 gcccacatcc ttatccgacc attgtgtgtg agtttcagcg gatgattggc gaagaaacga
    15901 aagcgcagat tctggaaaga gaaggtcgcc tgccggatgc cgttatcgcc tgtgttggcg
    15961 gcggttcgaa tgccatcggc atgtttgcag atttcatcaa cgaaaccaac gtcggcctga
    16021 ttggtgtgga gccgggtggt cacggtatcg aaactggcga gcacggcgca ccgctaaaac
    16081 atggtcgcgt gggcatttat ttcggtatga aagcgccgat gacgcaaacc gaagacgggc
    16141 agattgaaga atcttactcc atctccgccg gactggattt cccgtctgtc ggcccacaac
    16201 acgcgtatct taacagcact ggacgcgctg attacgtgtc tattaccgat gatgaagccc
    16261 ttgaagcctt caaaacgctg tgcctgcacg aagggattat cccggcgctg gagtcttccc
    16321 acgccctggc ccatgcgctg aaaatgatgc gcgaaacccc ggaaaaagag cagctactgg
    16381 tggttaacct ttccggtcgc ggcgataaag acatcttcac cgttcacgat attttgaaat
    16441 cacgagggga aatctgatgg aacgctacga atctctgttt gcccagttga aggagcgcaa
    16501 agaaggcgca ttcgttcctt tcgtcacgct cggggatccg ggcattgagc agtcattgaa
    16561 aattatcgat acgctaattg aagccggtgc tgacgcgctg gagttaggta tccccttctc
    16621 cgacccactg gcggatggcc cgaccattca aaacgccact ctgcgcgcct ttgcggcagg
    16681 tgtgactccg gcacaatgtt ttgaaatgct ggcactgatt cgccagaaac acccgaccat
    16741 tcccattggc ctgttgatgt atgccaatct ggtgtttaac aaaggcattg atgagtttta
    16801 tgcccagagc gagaaagtcg gcgtcgattc ggtgctggtt gccgatgtgc cagttgaaga
    16861 gtccgcgccc ttccgccagg ccgcgttgcg ccataatgtc gcacctatct tcatctgccc
    16921 gccaaatgcc gatgacgacc tgctgcgcca gatagcctct tacggtcgtg gttacaccta
    16981 tttgctgtca cgagcgggcg tgaccggcgc agaaaaccgc gccgcgttac ccctcaatca
    17041 tctggttgcg aagctgaaag agtacaacgc tgcacctcca ttgcaggggt ttggtatttc
    17101 cgccccggat caggtaaaag cagcgattga tgcaggagct gcgggcgcga tttctggttc
    17161 ggccattgtt aaaatcatcg agcaacatat taatgagcca gagaaaatgc tggcggcact
    17221 gaaagttttt gtacaaccga tgaaagcggc gacgcgcagt taatcccaca gccgccagtt
    17281 ccgttggcgg cattttaact ttctttaatg aagccggaaa aatcctaaat tcatttaata
    17341 tttatctttt taccgcttcg cttacccctg tcgaacgtca acttacgtca tttttccgcc
    17401 caacagtaat ataatcaaac aaattaatcc cgcaacataa caccagtaaa atcaataatt
    17461 ttctctaagt cacttattcc tcagata
//
LOCUS       DAJTMK010000083        16922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTMK010000083 DAJTMK010000000
VERSION     DAJTMK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.084"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..3025
                     /gene="ygfK"
                     /locus_tag="OO135_002330"
     CDS             <1..3025
                     /gene="ygfK"
                     /locus_tag="OO135_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR5398156.1"
                     /translation="SIFGIPEQQFYSPVKGKTVSVFGETCATPVGPAAGPHTQLAQNI
                     VTSWLTGGRFIELKTVQILDRLELEKPCIDAEDECFNTEWSTEFTLLKAWDEYLKAWF
                     ALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQPPMQQFIDNMMDASDHPKFAQYRDT
                     LNKLLQDDAFLARHGLQEKRESLQALPARIPTSMVQGVTLSTMHGCPPHEIEAICRYM
                     LEEKGLNTFVKLNPTLLGYARVREILDICGFGYIGLKEESFDHDLKLTQALEMLERLM
                     ALAKEKSLGFGVKLTNTLGTINNKGALPGEEMYMSGRALFPLSINVAAVLSRAFDGKL
                     LISYSGGASQLTIRDIFDTGIRPITMATDLLKPGGYLRLSACMRELEGSDAWGLDHVD
                     VERLNRLAADALTMEYTQKHWKPEERIEVAEDLPLTDCYVAPCVTACAIKQDIPEYIR
                     LLGEHRYADALELIYQRNALPAITGHICDHQCQYNCTRLDYDSALNIRELKKVALEKG
                     WDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAGYFLARAGHPVTLFEREANAGGVVKN
                     IIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHYVLIATGTDKNSG
                     VKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCARAALRVPGVEKAT
                     IVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPECFDADGTLTLRVMSLGEPDEKGRR
                     RPVETNEIVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDHNGETRLTDVFMI
                     GDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNNKYWNNVNPAEIYQRKGDISITL
                     MNSDDRDAFVAQEAARCLECNYVCSKCVDVCPNRANVSIAVPGFQNRFQTLHLDAYCN
                     ECGNCAQFCPWNCKPYKDKITVFSLAQDFDNSSNPGFLVEDCRVRVRLNNQSWVLNID
                     SEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            3028..4362
                     /gene="ssnA"
                     /locus_tag="OO135_002331"
     CDS             3028..4362
                     /gene="ssnA"
                     /locus_tag="OO135_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR5398157.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIAIENDVIVAIGDALTQRYP
                     DASYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDE
                     ESLYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTD
                     RNNGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREA
                     VKATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIA
                     LLNQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDT
                     GGPLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGH
                     IAFGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            4413..5192
                     /locus_tag="OO135_002332"
     CDS             4413..5192
                     /locus_tag="OO135_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR5398158.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYPACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAEEEAV"
     gene            5189..8071
                     /locus_tag="OO135_002333"
     CDS             5189..8071
                     /locus_tag="OO135_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR5398159.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HTAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADADADVIIERTYNSTQA
                     QQCPTETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFG
                     SKQDILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAV
                     KMDFRANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAP
                     KGNFAITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCA
                     LEEILRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFI
                     VHSGGADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGN
                     AARLAAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGF
                     GSLVGTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQI
                     YGATLRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPF
                     GAKSISEIGVNGAAPAIATAIHDACGIWLREWHFTPERILTALEKI"
     gene            8236..9636
                     /gene="xanQ"
                     /locus_tag="OO135_002334"
     CDS             8236..9636
                     /gene="xanQ"
                     /locus_tag="OO135_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCR5398160.1"
                     /translation="MSDINHAGSDLIFELEDLPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSIIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            9654..10970
                     /gene="guaD"
                     /locus_tag="OO135_002335"
     CDS             9654..10970
                     /gene="guaD"
                     /locus_tag="OO135_002335"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCR5398161.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLGVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSSYRTYVDGRLVYERN"
     gene            11006..12373
                     /gene="ghxQ"
                     /locus_tag="OO135_002336"
                     /gene_synonym="ygfQ"
     CDS             11006..12373
                     /gene="ghxQ"
                     /locus_tag="OO135_002336"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HCR5398162.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(12409..12897)
                     /locus_tag="OO135_002337"
     CDS             complement(12409..12897)
                     /locus_tag="OO135_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5398163.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVECCPTQALQLLTERELRRVLQQRIVASGEN
                     PL"
     gene            complement(12897..14815)
                     /gene="ygfT"
                     /locus_tag="OO135_002338"
                     /pseudo
     CDS             complement(12897..14815)
                     /gene="ygfT"
                     /locus_tag="OO135_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
     gene            15251..16699
                     /gene="uacT"
                     /locus_tag="OO135_002339"
     CDS             15251..16699
                     /gene="uacT"
                     /locus_tag="OO135_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5398164.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            16701..16826
                     /locus_tag="OO135_002340"
     CDS             16701..16826
                     /locus_tag="OO135_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398165.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(16823..16894)
                     /locus_tag="OO135_002341"
     CDS             complement(16823..16894)
                     /locus_tag="OO135_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398166.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
BASE COUNT         3997 a         4343 c         4305 g         4277 t
ORIGIN      
        1 ctcaatcttt ggtattcccg agcaacagtt ttactcacct gtaaaaggta aaactgttag
       61 cgtcttcggt gaaacctgtg ccactcccgt cggccctgcc gctggcccac acacgcagct
      121 cgcgcaaaat attgtcactt cctggctgac tggcggacgt ttcatcgaac taaaaaccgt
      181 ccaaattctt gaccgcctgg agctggaaaa gccctgtatt gatgccgaag acgagtgctt
      241 taacaccgaa tggtctaccg aatttaccct gcttaaagcc tgggatgaat acctcaaagc
      301 ctggtttgcc ctgcaccttc tcgaagcgat gttccaacct tctgattccg gtaaatcgtt
      361 catctttaat atgagcgtcg gttacaacct cgaaggtatt aagcaaccgc caatgcaaca
      421 gttcatcgac aatatgatgg acgcatctga ccatccgaaa ttcgcgcaat atcgcgatac
      481 gctgaataaa ttactccagg atgacgcatt tttagcccgc cacggattgc aggaaaaacg
      541 cgaaagcttg caagccttac ccgctcgcat ccccaccagt atggtgcagg gcgtcaccct
      601 ctccaccatg cacggctgtc ctccgcatga aatcgaagcc atttgccgct acatgctgga
      661 agaaaaaggg ctcaacacct ttgtgaaact caacccgaca ttactggggt acgcgcgtgt
      721 tcgtgagatc ctcgatatct gcggtttcgg ttacataggc ttaaaagaag agtcatttga
      781 tcacgacctc aagctgacgc aagcgctgga aatgctggaa cgcctgatgg cactggcaaa
      841 agaaaaatca ctcggctttg gcgtaaaact gacgaacact ctcggcacca tcaacaataa
      901 aggcgcactg cctggtgaag agatgtatat gtcaggccgt gcgctgttcc cgctctccat
      961 caatgttgca gcagttctct ctcgcgcctt tgacggcaaa ctgctcattt cttattccgg
     1021 tggtgccagt cagctgacta tccgcgatat ttttgatact ggtattcgcc ctattactat
     1081 ggcaaccgat ctgctgaaac ctggcggcta tctgcgctta agtgcctgca tgcgcgagct
     1141 ggaaggctcc gacgcctggg gacttgacca tgttgacgtc gaacgactga accgactggc
     1201 agcagacgcg ttaaccatgg aatacaccca gaaacactgg aagccagaag agcgtattga
     1261 agtggcagaa gacctgccgc tgaccgattg ctacgttgcc ccctgtgtta ctgcctgcgc
     1321 tatcaagcaa gatattccgg aatacatccg tctgcttggc gaacaccgct atgccgacgc
     1381 gctggaactc atctaccaac gcaacgctct gcccgccatt accggtcata tttgcgatca
     1441 ccagtgccaa tacaactgta cccgcctgga ttacgacagt gcgctgaata tccgcgaact
     1501 gaaaaaagtc gcgctggaaa aaggttggga tgaatataag caacgctggc acaaaccagc
     1561 cggttctggt tcacgccatc cggttgccgt gattggtgca ggtccggcgg gtctggcagc
     1621 aggttacttc cttgccagag cgggccatcc ggttacgctg tttgaacgcg aagccaatgc
     1681 gggcggcgtg gtgaaaaata tcattcctca gttccgtatt cctgcagagt taattcagca
     1741 cgatatcgat tttgttgccg ctcacggcgt gaaatttgag tatggctgct cacccgattt
     1801 gaccgttgaa cagttaaaaa atcagggctt ccactatgtt ctgattgcca ccggcactga
     1861 taaaaatagc ggtgtgaaac tggcgggcga caaccaaaat gtctggaaat cactcccctt
     1921 cctgcgtgaa tacaacaagg gtacagcgct caagctgggc aaacatgtgg tcgttgtcgg
     1981 ggcgggtaac accgcaatgg actgcgctcg tgcggcgtta cgcgttccag gcgtagaaaa
     2041 agcaacgatc gtttaccgtc gttcactgca agagatgccc gcatggcgcg aagagtatga
     2101 agaagcgttg cacgacggcg tggagttccg tttcctgaat aatccggaat gtttcgatgc
     2161 tgatggcacc ttaaccttgc gcgttatgtc gcttggcgaa cccgatgaga aaggtcgtcg
     2221 ccgtccggtt gaaaccaacg aaatagtaac actgcatgta gacagtctga tcaccgccat
     2281 cggtgaacag caggatactg aagccctgaa tgcgatgggc gtaccgctgg acaaaaacgg
     2341 ctggccagac gtcgaccata atggcgaaac tcgtctgact gacgtcttta tgatcggcga
     2401 cgtacagcgc ggaccatcct ccattgtcgc tgctgtcgga accgcgcgtc gggcgaccga
     2461 tgccatcctt agtcgggaaa atatccgttc ccaccagaac aataaatact ggaacaacgt
     2521 caatccggcg gaaatctatc aacgtaaagg cgatatctct atcacgctga tgaacagtga
     2581 cgatcgtgat gcgtttgtcg cgcaggaagc tgctcgctgc cttgaatgta actacgtttg
     2641 cagcaagtgc gtggatgtct gcccgaaccg cgccaacgtc tccattgcag tccctggctt
     2701 ccagaataga ttccagacgc tgcacctcga cgcttactgt aacgaatgcg gcaactgcgc
     2761 tcagttctgt ccgtggaact gtaaaccata caaagacaaa atcaccgtct tcagcctggc
     2821 gcaagacttt gataacagca gcaacccagg cttccttgtg gaagattgcc gggtacgagt
     2881 acgtctgaat aaccaaagct gggtgttaaa catcgacagc gaaggtcagt ttaacaacgt
     2941 accaccggag ctgaacgata tgtgccgcat catcagccat gtccaccagc atcatcatta
     3001 tctgctgggc cgcgtggagg tgtaatcatg ttgattctga agaatgtcac cgcagtgcag
     3061 ttacacccgg cgaaagtgca ggaaggcgtt gatatcgcta tcgctatcga aaatgacgtg
     3121 attgtcgcta tcggcgatgc cctgacgcaa cgctaccccg atgccagcta caaagagatg
     3181 catggtcgga ttgtgatgcc gggaattgtc tgctcgcaca accattttta ctcggggctt
     3241 tcccgcggaa ttatggcaaa catcgccccc tgcccggatt tcatctcaac gctgaaaaat
     3301 ctctggtggc ggctcgatcg cgcccttgat gaagagtcgc tctattacag cggactgatt
     3361 tgttccctgg aagcgattaa gagcggatgt acatcggtta tcgatcacca tgcctctccg
     3421 gcgtatatcg gcgggtcgct ctccacattg cgcgacgcat ttttaaaagt tggcctgcgc
     3481 gcaatgacct gttttgaaac tactgaccgt aacaacggca tcaaagagtt gcaggaaggt
     3541 gtagaagaaa acatccgttt cgcccgtttg attgatgagg cgaagaaagc gacaagcgag
     3601 ccgtatctgg tggaagcaca tatcggtgct cacgcgccgt ttaccgtgcc ggatgccggt
     3661 ctggagatgc tgcgtgaagc cgtgaaagcc acaggccgtg gtttgcatat tcacgctgcg
     3721 gaagaccttt acgacgtttc ctacagtcac cactggtacg gcaaagacct gctggcacga
     3781 ctggcgcaat tcgatctcat cgacagcaaa acgctggtcg ctcatgggct gtacttgtcg
     3841 aaagatgaca tcgccctact caatcagcgc gatgcgttcc tggtgcataa cgcccgttca
     3901 aacatgaaca accatgtcgg ctacaaccat caccttagcg acatccgcaa cctggcattg
     3961 ggaacggacg gcattggttc ggacatgttt gaagagatga aatttgcctt ctttaaacat
     4021 cgcgatacgg gtggtccgct gtggcctgac agttttgcca aagccctgac taacggtaac
     4081 gaactgatga gccgcaactt tggcgcgaaa tttggacttc tggaagccgg ttacaaagct
     4141 gatttaacca tttgcgatta caactcaccg acgccgctgc tggcagacaa tatcgccggg
     4201 catatcgctt tcggtatggg ctcaggcagc gttcacagcg tgatggtcaa tggtgtgatg
     4261 gtctatgaag accgtcagtt taacttcgat tgcgattcca tttatgcaca agccagaaaa
     4321 gccgctgcca gtatgtggcg tcggatggat gcgctggcat aaatgacaga tgccctcttc
     4381 ccgcagggaa gagggttaaa taaggaaaga agatgattga acaatttttc aggcccgact
     4441 ctgtcgaaca ggcgctggaa ctgaagcgcc gctaccagga tgaagccgtc tggttcgccg
     4501 ggggcagcaa actcaatgct acaccaaccc gtaccgataa aaagattgcc atttccttac
     4561 aggatctgga actggactgg gttgactggg ataacggtgc actgcggatt ggcgcaatgt
     4621 ctcgcttgca gccactgcgt gatgcgcgat ttattcctgc agcgctgcgt gaagccctcg
     4681 gttttgttta ctcacgccat gttcgtaatc agtcgaccat tggtggtgaa atcgccgccc
     4741 ggcaggaaga gtcggtgctg cttcccgtcc tgttggcact ggatgctgaa ctggtttttg
     4801 gcaacggcga aacgctgtca atcgaggact acccggcctg cccatgcgat cgcctgctaa
     4861 ccgaaattat cattaaagat ccgtatcgca cctgtgcgac ccgcaaaatt agccgttctc
     4921 aggcaggttt aaccgtcgtg acggcagccg ttgcaatgac agaccacgac ggtatgcgaa
     4981 ttgcgctgga tggcgtcgcc agtaaagcac tgcgtctgca tgatgtcgaa aaacaaaatc
     5041 tggaaggcaa tgcacttgaa caggctgtcg ccaacgccat tttcccgcag gaagatttgc
     5101 ggggcagcgt ggcctataaa cgctatatca cgggagttct ggtagccgac ctgtatgccg
     5161 actgccaaca ggctgaggag gaagccgtat gatcatccac tttactttaa atggcgcgcc
     5221 tcaggagcta accgttaatc caggcgaaaa cgtgcaaaag ctgttgttta acatgggaat
     5281 gcactctgta cgcaacagtg atgatggctt cgggtttgct ggttctgacg caataatctt
     5341 taacggtaat atcgttaacg cgtccttgct tattgccgca cagttagaga aggcagatat
     5401 tcgtaccgca gaatctctgg gcaaatggaa cgagttaagt ctggttcaac aggcaatggt
     5461 tgatgttggc gtggtgcagt ctggttataa cgatccagct gcagctctga ttatcaccga
     5521 tcttctcgat cgcatcgccg cacctacccg cgaagagatc gacgacgcgc tttctggttt
     5581 gttcagccgc gatgctggct ggcagcaata ctatcaggtc attgaactgg cggttgcacg
     5641 taaaaataat ccgcaggcca ccattgatat cgctccgact ttccgtgacg acctagaagt
     5701 cattggtaag cattatccta aaactgatgc cgcgaaaatg gtgcaggcga aaccctgcta
     5761 tgttgaagac cgcgtaacgg ctgacgcctg cgtcattaaa atgttacgta gcccacacgc
     5821 tcacgcactg attactcatc tggatgtcag caaagctgaa gccttaccgg gcgtcgttca
     5881 cgttattact cacctgaatt gcccggatat ctactatacc ccgggtggtc agagcgcacc
     5941 ggaaccatca ccgcttgacc gccgtatgtt cggcaagaaa atgcgtcacg tcggcgatcg
     6001 tgttgctgcg gtcgtcgcag aaagtgaaga aattgcgctc gaagcactga agctcatcga
     6061 cgttgaatat gaagtgctta agccggtaat gtcgatcgac gaagcaatgg cggaagatgc
     6121 gcctgtcgtg cacgatgaac cggtggtgta tgttgctggt gcgccagata ctctggaaga
     6181 cgataacagc catacagccc agcgcggcga gcatatgatc atcaacttcc cgatcggttc
     6241 tcgccctcgc aaaaatatcg ccgccagtat tcatggtcat attggcgata tggacaaagg
     6301 ctttgccgat gccgatgccg atgccgatgt gatcattgag cgaacctata actcaacgca
     6361 ggcacagcag tgcccgactg aaacacatat ctgctttacc cggatggacg gcgatcgtct
     6421 ggttatccac gcctccaccc aggtaccatg gcacttacgc cgccaggtcg cgcgcctcgt
     6481 gggcatgaaa cagcataaag ttcatgtcat taaagagcga gttggcggcg gttttggttc
     6541 caaacaggac atcctgctgg aagaagtgtg cgcctgggca acctgcgtga ccgggcgtcc
     6601 ggtactgttc cgctacaccc gtgaagaaga gtttattgct aacacctctc gtcacgtcgc
     6661 gaaagtcacc gtcaaactgg gagcgaaaaa agatggtcgc ctgacggcag tgaagatgga
     6721 tttccgcgcc aacactggcc cttacggcaa ccactcactc accgtaccgt gtaacggacc
     6781 ggcgctgtcg ctgccgttat atccgtgcga taacgtcgat ttccaggtca ccacctacta
     6841 cagcaacatt tgcccaaatg gtgcttatca gggttatggc gcaccgaaag gtaacttcgc
     6901 tatcaccatg gcattagcgg aactggctga acagttacag atcgaccaac tggaaattat
     6961 cgaacgtaac cgggtacacg aagggcaaga gctgaaaatt ctcggtgcaa tcggtgaagg
     7021 taaagcgccg acctccgttc cttccgccgc cagctgtgca ctggaagaga tcctgcgtca
     7081 gggtcgcgag atgatccaat ggtcgtcacc gaaaccgcaa aatggtgact ggcacatcgg
     7141 tcgcggcgtc gccattatca tgcagaaatc gggtatcccg gatatcgatc aggctaactg
     7201 catgatcaaa ctggaatcgg acggcacctt tatcgttcat tctggcggtg cggatattgg
     7261 tactggtctg gatactgtag tgacgaaact ggcagcagaa gtgctgcact gcccaccgca
     7321 ggacgtgcat gttatctccg gtgataccga tcatgcgttg tttgataaag gcgcatatgc
     7381 ctcgtccggt acttgcttct cgggtaacgc ggcgcgtttg gcagcggaaa atctacggga
     7441 gaaaattctg ttccacggcg cgcaaatgtt gggtgagcca gtggcagatg ttcaactagc
     7501 aacgccgggc gtcgtgcgcg gcaagaaagg cgaagtcagc ttcggggata ttgctcataa
     7561 aggcgaaacc ggcaccggct ttggttcact ggtgggaact ggcagttata tcacgcctga
     7621 tttcgccttc ccgtatggcg caaacttcgc tgaagttgcc gtcaacacgc gtacgggtga
     7681 aatccgcctg gataaattct acgccttgct ggactgcggt acaccggtca acccggaatt
     7741 agcattggga caaatctacg gtgctaccct gcgagctatc ggccacagta tgagcgaaga
     7801 gatcatttat gacgccgaag gtcacccgtt aacgcgtgat ttacgcagct acggcgcacc
     7861 gaaaattggt gacattccgc gtgatttccg cgcagtgctg gtgccgagcg acgataaagt
     7921 cggcccgttc ggggcgaaat cgatctcgga aatcggtgta aatggcgcag ctccggcgat
     7981 tgctaccgca attcacgatg catgcggcat ctggttacgc gaatggcatt tcacaccgga
     8041 gagaatactc accgcgctgg aaaagatata aataataaat ggccgcttcg gcggcctgtt
     8101 tctctgtttg gacgatgact aaatagtgac tgcgaaaatg agataagccg ttctcaaggc
     8161 gtcctgcgcc ccaaattcgt gggaatttat tttactaatt cagatgatca aatttacttt
     8221 aaaaggagtg aagggatgtc tgatataaac catgcaggtt ctgaccttat atttgaactg
     8281 gaggatctcc ctccctttca tcaggctctc gttggtgcca ttacccatct gttggcaatt
     8341 ttcgttccga tggtaacccc cgcgttaatc gtgggtgcgg ccttacagct ttccgctgaa
     8401 acaactgcct atcttgtttc aatggcgatg atcgcctctg gtattggtac ctggttacaa
     8461 gtaaaccgct acggcatcgt cggttctggc ctactctcaa ttcagtcagt caatttttca
     8521 tttgttacgg tcatgattgc gctgggcagc agcatgaaaa gcgacggttt tcacgaagag
     8581 ttaatcatgt cgtcgcttct tggcgtctcc ttcgttggcg catttctggt tgtcggctct
     8641 tcttttatcc tgccctattt acgtcgggtt attacgccta ctgtcagcgg tattgtggta
     8701 ctgatgatcg gcttaagcct gattaaagtc ggcattattg attttggtgg aggatttgct
     8761 gccaaaagca gcggtacctt cggcaattac gaacatctcg gcgttggttt attggttttg
     8821 attgtggtga tcggctttaa ctgctgtcgc agtccgttgc tacgcatggg agggatcgct
     8881 attgggctat gtgtcggcta tatcgcatcg ttatgcctgg gcatggtgga tttcagcagt
     8941 atgcgcaatt tgccgttaat caccatccca catccattca aatacggctt tagttttagc
     9001 ttccatcagt tcctggtggt tggcacgatt tatctgctta gcgtgctgga agcagtcggc
     9061 gatatcaccg ccacggcaat ggtttcccgc cgccccattc agggggaaga gtatcagtcc
     9121 cgactgaaag gcggcgtgct ggcagatggt ctggtttctg ttatcgcctc cgctgtcggt
     9181 tcattaccat taaccacgtt tgcgcaaaat aatggtgtta ttcagatgac tggcgtcgct
     9241 tcacgttatg tcgggcgaac catcgcggta atgctggtta tcctcggctt atttccgatg
     9301 attggcggct tcttcacgac cattccctcg gcagttctgg gaggcgcaat gacgttgatg
     9361 ttttccataa ttgcaatcgc agggattcgc atcatcatca ccaacggttt aaagcgccgt
     9421 gaaacactta ttgtcgccac ttctttaggt ttagggcttg gcgtctccta cgatcccgaa
     9481 atttttaaaa tattgccagc ctctatttat gtattagttg aaaaccctat ttgtgctggc
     9541 gggttaactg cgattttatt aaatattatc ctccctggtg gctaccgaca ggaaaacgtt
     9601 ctgcctggta ttacctcagc ggaagagatg gattaacagt aaaggagtca atgatgtcag
     9661 gagaacacac gttaaaagcg gtacgaggca gttttattga tgtcacccgt acggtagata
     9721 acccggaaga gattgcctct gcgctgcggt ttattgagga tggtttatta ctcattaaac
     9781 agggaaaagt ggaatggttt ggcgaatggg aagacggaaa gcatcaaatt cctgacacta
     9841 ttcgcgtgcg cgactatcgc ggcaaactga tagtaccggg ctttgtcgat acacatatcc
     9901 attatccgca aagtgaaatg gtgggggcct atggtgaaca attgctggag tggttgaata
     9961 aacacacctt ccctactgaa cgtcgttatg aggatttaga gtacgcccgc gaaatgtcgg
    10021 cgttcttcat caagcagctt ttacgtaacg gaaccaccac ggcgctggtg tttggcactg
    10081 ttcatccgca atccgttgat gcgctgtttg aagccgccag tcatatcaat atgcgtatga
    10141 ttgccggtaa ggtgatgatg gaccgcaacg caccggatta tctgctagac actgccgaaa
    10201 gcagctatca ccaaagcaaa gaactgatcg aacgctggca caaaaatggt cgtctgttgt
    10261 atgcgattac gccacgcttc gcccctacct catctcctga acagatggcg atggcgcaac
    10321 gcctgaaaga agaatatccg gatacgtggg tacataccca tctctgtgaa aacaaagatg
    10381 aaattgcctg ggtgaaatcg ctttatcctg accatgatgg ttatctgggt gtttaccatc
    10441 agtacggcct gaccggtaaa aactgtgtct ttgctcactg cgtccatctc gaagaaaaag
    10501 agtgggatcg tctcagcgaa accaaatcca gcattgcttt ctgtccgacc tccaaccttt
    10561 acctcggcag cggcttattc aacttgaaaa aagcatggca gaagaaagtt aaagtgggca
    10621 tgggaacgga tatcggtgcc ggaaccactt tcaacatgct gcaaacgctg aacgaagcct
    10681 acaaagtgtt gcaattacaa ggctatcgcc tctcggcata tgaagcgttt tacctggcca
    10741 cgctcggcgg agcgaaatct ctgggccttg acgatttgat tggcaacttt ttacctggca
    10801 aagaggctga tttcgtggtg atggaaccca ccgccactcc gctacagcag ctgcgctatg
    10861 acaactctgt ttctttagtc gacaaattgt tcgtgatgat gacgttgggc gatgaccgtt
    10921 cgagctaccg cacctacgtt gatggtcgtc tggtgtacga acgcaactaa taataaaact
    10981 ttaacatcct cgtgaggaca tcattatgtc tggagacatc ctacaaacac cggacgcacc
    11041 aaagccacag ggcgcgctgg ataattattt taaaattacc gctcgtggca gtaccgttcg
    11101 tcaggaagta ctggctggct taacgacctt tctggccatg gtttattccg ttatcgtcgt
    11161 tccgggaatg ctgggcaaag caggttttcc tcccgcagct gtgtttgttg ccacctgtct
    11221 ggtcgcgggc ttcggctcgt tgctgatggg attatgggcc aatttgccaa tggcgattgg
    11281 ttgcgcgatt tccttgacgg cgtttaccgc attcagtctg gtactcgggc aacaaattag
    11341 cgttcctgtc gcactgggcg cggtatttct gatgggcgtc atcttcaccg ccatttccgt
    11401 aaccggtgtg cgtacctgga tcttacgtaa tttgccgatg ggtatcgctc atggtacagg
    11461 tatcggtatt ggcctgtttc tgctgctgat tgctgctaac ggtgtgggta tggttatcaa
    11521 aaacccgatt gaaggcttgc cagtggcgct cggtgcgttt acctccttcc cggtgatgat
    11581 gagcttgctg gggctggcgg tcatcttcgg tctggagaag tgtcgcgtac ccggcgggat
    11641 cttgttggtg attattgcaa tttcgatcat cggcttaatc tttgatccag cggtgaagta
    11701 ccacggtctg gtggcgatgc caagcctgac tggcgaagat ggtaagtctc tgattttcag
    11761 cctcgatatt atgggtgcac tccagccaac tgtacttccg agtgtactgg cattggtgat
    11821 gaccgcagtg ttcgacgcta ctggcaccat ccgtgccgtc gccggtcagg cgaatttgtt
    11881 ggataaagac aaccagatca tcaacggcgg caaagccctg accagtgact cagtaagttc
    11941 aatattctcc ggcctggtgg gcgcagcgcc cgcagcggtt tatatcgaat cagcggcagg
    12001 aaccgccgcc gggggtaaaa cagggttgac cgcaaccgta gtgggggcgt tattcctgtt
    12061 aattctgttt ttatcaccgc tgtcattttt gatccctggt tacgccactg cccccgctct
    12121 gatgtacgtg ggtttgctga tgttaagtaa cgtctcgaag ctggatttca atgattttat
    12181 tgacgctatg gctggcctgg tgtgtgccgt gttcatcgtt ctgacttgta atattgttac
    12241 cggtattatg ctgggctttg tgacactggt cgtaggccgc gtctttgcac gcgaatggca
    12301 aaagctgaat attggtacag tgatcattac tgctgcactg gtcgcgtttt acgcgggtgg
    12361 ttgggcaatc taagagattc tccgcgcctt tcagcgcgga gatttctttc agagaggatt
    12421 ctcaccgctg gcaacaatac gctgctggag gactcgcctg agttctcttt cagtaagcaa
    12481 ctgcagcgcc tgtgtcgggc aacattcaac gcaggctggc ccctcttccc gctgttcaca
    12541 gagatcgcat ttcacgattt gttgccgggt atcccccggc aacgactgaa tggtgatcat
    12601 tccaaacggg cacgcactga cgcaactctg acagccaata caacgggcag aattggtctg
    12661 caccacctgc tcgcccatcg tcagcgcccc cacggggcaa gcgccaacac aaggggcgtt
    12721 ttcacactga tggcacatca ccggcgcact gatgctgtcc agtcgctgta ccttcagccg
    12781 gggtaaaaag acatcggcat tcaactcctg ttctgaaggg tgagcgacca cacaggctac
    12841 ttcacaagta cggcagccaa tacagtcagc cggattaacg ataattaacg atttcatcac
    12901 gatgccttcg tatcaaacag agttaacata tcgcgcgccg cctgccttcc tgcggccatt
    12961 gcagtgacaa ccagatccgc gccatgaact gcatccccac cagcaaagac ttttttcaga
    13021 tgcgtctggg taggtaaata cccgacgtca ccggtttgaa tcaggcccca tttatcgagt
    13081 ttaattccgc tgccctgcaa ccacggcatg gcatgcgcct ggaaaccaaa ggccataatg
    13141 agaacatcgg cgggcaattc aaactctgaa cccgcaaccg gacgaggacg acggcgacca
    13201 tccggccccg gctcacccat ggcggtacga atcaggccca ccgcagttaa acgcccatct
    13261 tcgtcacaag cgatatattg cggttgaaca ttgaactgaa actcgacacc ttcctcgcgc
    13321 gcattaacca cctctttgcg cgagcccggc atactgactt catcacgacg atacgcgcag
    13381 gtcacgctgg cggcattgag gcggatggaa gtccgcaaac aatccattgt cgtatcgcca
    13441 ccgccaatac cacgactcgc ttaccttcca cgtccgtcag cggatactct tcagactccg
    13501 gcaatcccat gagctggcgg gtatgggcag tcaggaacgg tagagcctga atgacacagg
    13561 gcgcatcttc atgcggcaga tctgctcgca tcatcccgta agtccccacg ccgatgaaaa
    13621 ctgcatcata ttcagaagtt aaatcgctaa aggtaatatc gcggccaatt tcacagttga
    13681 gatgaaagtc gattcccatt gcggtgaata tctctcgccg ctggcttaat accgttttat
    13741 cgagtttgaa aggaggaatg ccaaaagtca gcataccgcc aatttctgga tggcgatcaa
    13801 agacatcaac ctgaactcct gcgcgcgcca gaatatcagc acaccctaac cctgctggcc
    13861 cagtgccaat caccgccact ttttcgctac ggggaacaac tttgctgaca tcgggacgcc
    13921 agcccatcgc cagcgcagta tcggtgatgt agcgttccag attaccgata gtgactgcgc
    13981 cagagtgatc tttcaaagta catgctcctt cacaaagacg gtcctgtggg cataccctgc
    14041 cgcagatttc tggtaaggaa ctggtctggt ggcaaagttc tgccgcttca ataatatttc
    14101 cttcctgtac cagacggatg taatccggaa tagcgttatg cagcgggcaa tgccagttgc
    14161 agttagcttt ttcggcacaa taaacacagc ggtcactctc ataagtcgct tgcagtggat
    14221 ccagcccgca atagatttcg ccaaagtggg ttttccgttc actcgctgaa attttatctg
    14281 cgcctttacg cgagttaaca gggagcatcg ctgcactgcg agatggctga gcgtctgatg
    14341 acgcttttcc tgctgccgtt ttgcgctggc gggccacctt tatctgctgt agccctttat
    14401 cgtccatcag tcgtaacgcc tgtgttgggc agacttaaat acaggcttgc gtgccggaac
    14461 tgcgctggtt acaaaggtcg catttctgcg caatcgtatc gaccatctca acgacgccaa
    14521 aggggcaagc gattgcgcat cttttacaac caatacattt tttctcgtcc agttgtacgc
    14581 tatcggactg gaaagtcaga gcattaaccg gacaagccgt aacgcaaggg gcattgttgc
    14641 agtgatggca ggccaccgga ttcgcagcct ggcctttccc tacaacgtgg atacgcggtc
    14701 gaaagtcact gtgactcagc ggccagtttt cttgattgtg tgccaccgca caggcaattt
    14761 cacaagcatg acagcctata cattccgcag cttcagcagc gataaactta ttcatttgca
    14821 tccctttcat ttaatgagtt atgtcttctc aaatttcgcg atgcaataaa cgggcatata
    14881 ttaggtagca tgaccgtttt aattataatt ttgcgactga gttcaaatta ttgcccctca
    14941 atctatgccg ctttcattat ttcaacattg ccgatcaaaa gagtgctatc cagataaaaa
    15001 cgattaaatg cgagagtacg acatgccaga atgattaatg aaatatcaat atgagaaaat
    15061 tacagataat tccattgtca ggttctcttc tcattttcgt ggtttaacca cgattatgtg
    15121 attgcctccg caattcctgt ctctaactcc ccttcctcgc aaaaactggc actccacgag
    15181 catgtgttta gacagtttca ttaacgtaaa cggtttcttt ttactctggc gggcgaaagg
    15241 agaaacactg atgagcgcca tagattccca acttctctca tcttctgggc aagaccgccc
    15301 aactgatgag gttgaccgca tattatcacc aggaaagctg atcatactcg gtctgcaaca
    15361 cgtccttgtc atgtacgcag gtgcagtcgc tgttcctctt atgattggtg accgactggg
    15421 cctctcaaaa gaagctattg cgatgctcat tagctcggat ctcttttgct gcgggatcgt
    15481 cacattattg caatgtatcg gtatcggccg ctttatgggg atccgcctgc cggtgattat
    15541 gtcggtgacc tttgctgctg taacaccaat gatagccatt gggatgaacc cggatattgg
    15601 cctgctgggg atatttggtg ccactatcgc cgcgggtttt atcaccacat tattagcgcc
    15661 acttatcggt cgcttgatgc ctttattccc gccactggtt accggtgtgg ttattacttc
    15721 tgtcggactt agcatcattc aggtgggtat tgactgggcc gccggaggta aagggaatcc
    15781 gcaatatggt aatcccgttt atttaggtat ctcctttgcc gtcttaattt ttatcttgct
    15841 cattactcgc tatgcgaaag gatttatgtc caacgtcgcc gtattactgg ggattgtatt
    15901 tggcttttta ctttcgtgga tgatgaatga agtcaattta tccgggctac atgatgcctc
    15961 gtggtttgcg attgtcaccc cgatgtcgtt tggtatgccg attttcgatc ccgtttccat
    16021 tctgaccatg actgccgtgt taatcatcgt gtttatcgag tcgatgggga tgttcctggc
    16081 actgggtgaa atagtcggtc gtaaactctc ttcacacgat attattcgcg ggctgcgtgt
    16141 cgatggcgta gggacaatga taggcggcac gtttaacagc ttcccccaca cgtcattttc
    16201 tcaaaacgtt ggcctggtta gcgtgacgcg cgttcatagc cgctgggtgt gtatttcttc
    16261 gggaattata ttaatcctgt ttggcatggt gccaaaaatg gcggtgctgg tagcctccat
    16321 tccgcaattt gtgctgggcg gcgctggtct agtgatgttc ggcatggtac tggcgacagg
    16381 gattcgaatt ctgtcgcgct gtaactacac caccaaccgt tacaacctct atattgtggc
    16441 gatcagtctc ggcgttggca tgactccgac gctctctcac gatttctttt ctaagttacc
    16501 ggccgtactg caaccgctgc tacatagcgg cattatgctc gcaaccctta gcgccgttgt
    16561 gctgaacgtc ttctttaatg gctatcagca tcatgctgac ctggtgaagg aatccgtctc
    16621 tgataaagat ttaaaagtca ggacagtacg tatgtggctt ctgatgcgca agctgaagaa
    16681 aaatgagcat ggagaataat ataaatcttt taatgcgcgc tatattcagt ctgctgttgc
    16741 tttttactct ctctattcct gtcatttctg actgtgttgc aatggccatt gaaagtcgct
    16801 tcaaatatat gatgctactt ttttaaatgg tttttacctg tcggcatccg ctcaaaacgg
    16861 gcggttgtcg ataaacgctc acttggttaa tcatttcact cttcaattat ctataatgat
    16921 ga
//
LOCUS       DAJTMK010000084        16697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTMK010000084 DAJTMK010000000
VERSION     DAJTMK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.085"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(78..1439)
                     /gene="eutB"
                     /locus_tag="OO135_002342"
     CDS             complement(78..1439)
                     /gene="eutB"
                     /locus_tag="OO135_002342"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCR5398167.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(1451..2854)
                     /gene="eutA"
                     /locus_tag="OO135_002343"
     CDS             complement(1451..2854)
                     /gene="eutA"
                     /locus_tag="OO135_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5398168.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLEEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGSHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDVRSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(2851..4077)
                     /gene="eutH"
                     /locus_tag="OO135_002344"
     CDS             complement(2851..4077)
                     /gene="eutH"
                     /locus_tag="OO135_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR5398169.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKTLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(4294..5481)
                     /gene="eutG"
                     /locus_tag="OO135_002345"
     CDS             complement(4294..5481)
                     /gene="eutG"
                     /locus_tag="OO135_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR5398170.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGGIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTIAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPLTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQLGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(5471..6307)
                     /gene="eutJ"
                     /locus_tag="OO135_002346"
     CDS             complement(5471..6307)
                     /gene="eutJ"
                     /locus_tag="OO135_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR5398171.1"
                     /translation="MAHDEQWLTPRLQMAAMLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTSVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNVGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(6318..7721)
                     /locus_tag="OO135_002347"
     CDS             complement(6318..7721)
                     /locus_tag="OO135_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR5398172.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQEMRLLFVETTAEHPFAVIELMMTVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(7733..8020)
                     /gene="eutN"
                     /locus_tag="OO135_002348"
     CDS             complement(7733..8020)
                     /gene="eutN"
                     /locus_tag="OO135_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR5398173.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(8127..8420)
                     /gene="eutM"
                     /locus_tag="OO135_002349"
     CDS             complement(8127..8420)
                     /gene="eutM"
                     /locus_tag="OO135_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR5398174.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(8459..9475)
                     /gene="pta"
                     /locus_tag="OO135_002350"
     CDS             complement(8459..9475)
                     /gene="pta"
                     /locus_tag="OO135_002350"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5398175.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(9465..10274)
                     /gene="eutT"
                     /locus_tag="OO135_002351"
     CDS             complement(9465..10274)
                     /gene="eutT"
                     /locus_tag="OO135_002351"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR5398176.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMCADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            complement(10271..10972)
                     /gene="eutQ"
                     /locus_tag="OO135_002352"
     CDS             complement(10271..10972)
                     /gene="eutQ"
                     /locus_tag="OO135_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR5398177.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            complement(10947..11426)
                     /gene="eutP"
                     /locus_tag="OO135_002353"
     CDS             complement(10947..11426)
                     /gene="eutP"
                     /locus_tag="OO135_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5398178.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(11439..11774)
                     /gene="eutS"
                     /locus_tag="OO135_002354"
     CDS             complement(11439..11774)
                     /gene="eutS"
                     /locus_tag="OO135_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5398179.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(12083..14347)
                     /gene="maeB"
                     /locus_tag="OO135_002355"
     CDS             complement(12083..14347)
                     /gene="maeB"
                     /locus_tag="OO135_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5398180.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEA"
     gene            14636..15586
                     /gene="tal"
                     /locus_tag="OO135_002356"
     CDS             14636..15586
                     /gene="tal"
                     /locus_tag="OO135_002356"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5398181.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            15606..>16697
                     /locus_tag="OO135_002357"
     CDS             15606..>16697
                     /locus_tag="OO135_002357"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5398182.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYVNELQANPAKIATRKASQNTL"
BASE COUNT         3747 a         4855 c         4360 g         3735 t
ORIGIN      
        1 ttgtcccatt gacgccatca cgctgcgtac aatttcttca atctgttttt gatccatgat
       61 gtgttatctc cgcgtcatca gaagaacagt gacggatcgc ccgcccgttt ggtcaggcga
      121 ccgtttgcca taatgcccat gctttccagc cagcgttcaa actctggtga cgggcgcagg
      181 ttgagtaact gacgcacagt ggcggtatcg tggaatgcgg tggtctggta gttgagcatg
      241 atgtcatcac ccagcggcat ccccatgatg tagttgcagc ctgcggtggc gagcagaatc
      301 atcaggtttt cgttgaggtt ctggtcagcg tcagcgtggt tggtgtagca gcagtcacag
      361 cccatagaga tgccgctcag cttgcccata aagtgatctt ctaagccagc acggataatc
      421 tggcggtcgt tgtagagata ctccggccca ataaagccga ccacggtgtt gacgataaac
      481 ggatcgtaat gacgcgccag cccatagtta cgtgcttcca tcgtcacctg gtcagcgccg
      541 aagttagcgc cagcggatag cgcagagcct tgtccggttt cgaagtagag gcagttttcc
      601 ccggcgatac ggttgaactc tgcacccact gcgcgcgctt cgtcgagcat cgccagttcc
      661 acgccaaact ctttcagccc tttttcgctg ccacagatac tctggaaaat cagtccgcca
      721 ggcgcgccgc gacgaatcgc ttcgatctgg gtggtgacgt gcgccagtac gcagccctga
      781 gttgggatgt tgaatttgtc gatcacgcca taaatggtat ccagcacgcg gcttaagttt
      841 tccacgtcgt cagttaccgg gttgacgccg atcaccgcat cgcccacccc gaaggaaagc
      901 ccttcgtaga tttgcgcggc gatactttgc acgtcgtcac gagtgtcatt tggctgcaaa
      961 cgggcgctga aggtgcccgg aataccgatg gtggtattgg cctttttgat taccggcatt
     1021 ttcttcgcgc cgtagatcag gtccgcgttg gagcaaatct tcgctaccgc cgcgaccact
     1081 tccgaggtca gccctttgcg ggtaaaggca atgtcgtcca cgctagtttc atcgctcagc
     1141 acatactcac gcagttcgct gatgctccag tttttaatct ggttgtaggc cgtttcgtta
     1201 acatcgtcct gaatcagccg cgtcacgcag tcatcttcat aggcaatcac cggattattg
     1261 cggatgtccg ctacggtcat ttccgacaac acctgctttg ccgccacgcg ctcctgtgaa
     1321 cttgccgctg caacgcctgc cagcacatcc cccgaacgca gttcgttggc tttagccagc
     1381 acctctttta catccttaaa ctgatataca ttgccgaaca atgtggtctt tagtttcata
     1441 agtcgttccc tcaggaagga aatgcgagtg atttcaccgt caccggcaca accgatccgc
     1501 caaaaagagg cgtaccaatg tcgatatagt cccccgcgcg gacaatcact tcgtcaatga
     1561 ctgccaacgg gagttgttgt agctgtgggc gcaacaacat gcccagagct ttaccaaagt
     1621 cctgcccggc caccaccagc aggggatgcg gattcggaaa acgcgcgacg aaatcgacca
     1681 gcgcgttgat gaccgtcagt accgcggcgt aacgcacagg cagcgaggcg ggaagcgcca
     1741 gcacgtacgc gtcagttttg ggatcaagat ccagctgaag cagcgcctgt tgccaggcac
     1801 tcaccagatc cgtttcatca attgggatcg ccaccggcaa attgcgcagc ggcagttgta
     1861 cgccctccag ccagatagtg ctgccagaga gcgaaagggt atgtgcgccc gcgccaatca
     1921 ccgtggcgcg tacggtttgc gccggaaact gcacattcat ctcacgcagg cgcggatggt
     1981 catgcagcgc cgttgccagc agcgggccaa tatcggcaaa acagaacgga tcggcgggct
     2041 ggtggcgata acattcgccc acgccgccag aaagcgtaat gatttcgggc gtaacacctg
     2101 cgggcagcaa accggtttgc attaatgcct gcgcgagcgg cgaaagcgtt ccgtcaatca
     2161 cttcgacaat cagttctgcc atccgccggg taacctgcac cagctgcgcg ccggtcagcg
     2221 aacggacgtc agtgcctgca ccgaagcact catccacaat catctgcccc ggtttatgag
     2281 cgtaaaccac gcgcccctgg ctgtcggttt ccagcaggcg accaccgacg ttgaggcagg
     2341 cagtgccgct gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca ccgccgatgt
     2401 cgatattcag tacccgacac agccgttgtt cagaaagggt ttgcgccccg gcaccgtgac
     2461 cggcgatcac ggattcgagg tgcgacccgg cgctggcgac gacaaaatcg cccagcgact
     2521 gagagagcgc catcaccgcc gggcgagcat tgcgggtttt cgcgctttca ccggtgatga
     2581 tgatggcacc agaatcaacg ctttccggcg caatgcccgc agcctgatat tgctctaaaa
     2641 tcagcgactt cagttccgct tcttctaaac caccctgttt atcgacaggg gtaaagaaca
     2701 ccgggctttg ccagctaatt tcgcgtttga tgaattcgta gcgcggcacc tgcgacaccg
     2761 ccgcacggtt aaccagctcc aggtgggaga aaatcacctg ggtggtggtg gtgccgatat
     2821 cgataccgac gctcagtagc tggcgagtgt tcacgattgt gcctccgctt cggttttagc
     2881 tgcggtcgcg tcatcttttg gcaccagcat catcgccacg ccaatcgccg ttacgccgcc
     2941 gatcaacttg ccgacaatca tcgggaagat catggcattc atgttggcag cggcgaaccc
     3001 taagtggtcg cccagggcga aagcagcaga aacggcgaag gcgcagttga tgactttgcc
     3061 gcgggtatcc atctgcttca tcatgccgaa catcgggatg ttgttggcaa gcgttgccac
     3121 catgccggct gccgcgatgt tgttcatatt cagcacttta ccgacgctca tcagcgtttt
     3181 ttcaaaccag cgagtcagca gcagcaccat cggatacgcc cctaacagaa cgcaggagat
     3241 agaaccgata acttcaatgg cgcgcatcac ctcaccgggt ttatcgccag gggccataaa
     3301 gataggatcc agaccgggga tcagttccca gccaagcagg aatttcacta ccgcagcggc
     3361 aagaccgagg gtgatcaatg caacgaggaa tttggcgaag atctggaagc cgttgatcat
     3421 tttttccggg atgaatttca gccccagcgc caccagaatc gcaacaatga tcaccgggat
     3481 catgttcatc aggatcaggg cgaaagtgaa ttccaccggc tgaccgttga tctgcacacc
     3541 ggagtacata gcaaccagac caccagcaat acagccaatc ggaatggtca caatgcccgc
     3601 cagcacgccg agcgccagat aacgacggtc agaaggttcg ataatgccga gcgccaccgg
     3661 aatggaaaac acaatcgttg gccccatcat cgacccaaga attaacccag agtatagcca
     3721 cgctgctacg tcgccgcccg ccagctcttt ggcgaggaag aagccgccca tatcgcacgc
     3781 cagcagcgtt ccggcgaaca ttgacgggtt tgcgccgagc atttcgtaaa ccgggataat
     3841 taccggcccg agtacgtgag ccagtaccgg tgccagcgcg gtcataccga ccatcgccag
     3901 acccagagcg cccattgcca taaagccttc ttcgaactga ccgcctgatc cttcgatact
     3961 tttaccgaac ttaccgagga aacgagcaga accgccgaac tgcgacagga tcctgtctac
     4021 ggcagctatc agcataaaga acatcatgat gtacatgatg atttcgttaa ttcccatcgc
     4081 ctttactccc tgttagttgt tatttattgg cggatgcggc gtaaacgcct tatccgccct
     4141 acatgtgcaa tcccgaaggc cggatcagac gcggcaagcg tcgcatccgg caattgcacc
     4201 gcgccactgg cggatgcggc gtgaacgcct tatccgccct acatgtgcaa tcccgtaggc
     4261 tcgataagac gcggcaagcg tcgcatccgg catttattgc gccgctgcgt acaggccgac
     4321 aatctgctcc aggctggcgg tacgcgggtt actgcgcaga caaatatctt ccagcgcggc
     4381 ctgcgcccat gcgccgtaat gcgcagatgt cgcaccaaca tcgcccagtc gtttaccaat
     4441 cccaacttcc gcaatcagct cacttaccgc gttaatagcg tcacgatcgt cggatttttt
     4501 agttcgcagt gcccgaccaa tctgactaaa gcgttcacga caaaccatcc ggttaaattc
     4561 catcaccgtt ggcagcaaca tggcgttcgc cagaccgtgt ggaatatgca gcgccgcgcc
     4621 cagctgatgc gccatcgcgt ggcacaaccc aagacccgca ctggaaaacg ccattcccgc
     4681 catacatgaa gccagcaaca tgctctcgcg cgcggcaagg tcgtgaccgt agcccaccgc
     4741 tttcggcagc gatttgccaa tcatcgcaat agcgccaatc gccaggctgt cggtaagcgg
     4801 tgtagcgttc agggcgctgt atgcttcaat ggcatgggtt aacgcatcaa tgccggtcat
     4861 cgccgtgaca tgcgacggca caccttcggt caatgcggcg tcgaggatcg ccacatccgg
     4921 catcagcgag gcatgggcta acacctgctt gcgcccgctc accgcgtcga taatcaccgt
     4981 tacattggtg gtttcagagc cggttccggc gatcgttgga atggcaatca gcggcaagcg
     5041 cggttgcaga acgctggttt ctgacatctc tgccagcgtg ctatccgggt tcgtcaccag
     5101 caacgccacg gctttcgccg catccagcac cgagccgccg ccaaatgcga tccccccatc
     5161 acagcctgac tcacgcaact gcgccacggc tgcacacacg tcggtgatgc acggttcgcc
     5221 caccggacat ggccagagcg tcatggcgat gcctttaaca gccaggctgc gcgtcagccc
     5281 ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc acgaacagat gtttcagccc
     5341 acgcgtttgc gcttgctgtc cgcaactgct caccgagccc ggaccgcaaa gcgtcaccgg
     5401 tggaacgcta aatgttttta cccgttgcag attcagggta tcgaacgcct gaaagagcgc
     5461 ggtctgcaat tcattttgca tatagcccct ccgctttctc tcgcccacta ctggcgatcg
     5521 ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac ctgtaacgcc gggaattgtt
     5581 tgcgaaacag ttccgccacg cccggttgca tacaagagcc gcccgccagc cataaatcag
     5641 taatgccctg cccttcaata tgccgggcga caatgtccgc cattttttcg tacaccggtt
     5701 tcaccgtagg ccaaatctct tcaccgtgac cgcgcttgta ctgctccgcc tcttccagcg
     5761 aaatacggcg atttccggcg agagtcagag agatgtgatg tccgccggtt gcttcatccg
     5821 ccgagtacgt caccttgccc tttttcacga tggcaatgcc ggtagtgccg ccgccgatat
     5881 ccactacacc gacgttgtcc agttgcagca gatccgcaac tgcggttggc tcatccagca
     5941 catggctcac ttccagtcca gcagactcca gcacgttgat ggaaatacgc gggtccgtac
     6001 ctggaggaaa tgaagtcgcc gcatggctaa aacggcgacc gaattgctgc tcgagcgtgt
     6061 cgagatggcg acgaacgctg gtgacagcgc cgaagaaatc ccagacgatg ccgtcgcgga
     6121 ccacatcggc ccagtcgagg cataccgcca ccggctgacc gtcgcggtcg acaaccatcg
     6181 acaccacatc gcaggtgccc agatccaccc ccagccacag cggagattcc gtcgcggcgg
     6241 gcgtctggtt acacagcatt gccgccattt gcagacgtgg ggtgagccat tgttcgtcgt
     6301 gcgccatctg ttactcctta aacaatgcga aacgcatcga ctaatacaca gcgacgcaga
     6361 cggacaaacg tacgcgcgct ggttacccct tcaccggttg gcgtggtgat ggtcatggtg
     6421 gtccagcctt ccccgcccag ccccagcccg gcaatgcacg gtccgttctt aacgaaaatg
     6481 ctggtatcaa tagcattcgc catctggttc atgttttcga tgttgcgcga gtgcattgcc
     6541 gccgtgtggt ggcaaccgcc ttccagtttc accgccagcg caatggcatc cgccacgttg
     6601 gcgacgcgca cgacgggcag caccgtcatc atcagttcga tcacggcaaa cggatgttct
     6661 gcagtggttt ccacaaacag caaacgcatt tcttgcggca cgttcaggcc gattgccgcc
     6721 gcgattttcg ccgcatcgcg accaacccag tcacggctga cggtgccttt tccgcgctcg
     6781 tcgatatttt tcagcaacac cggttgcagc tgctgcgcct gttctgcggt cagtttcacc
     6841 gcgtgctggc cttccatcag acgcatcagt tcatcggcta cgctatcaac aacaatcagt
     6901 accttttcgt cggcacaaat gatgttgtta tcgaaagaag cgcctttgac gatggactga
     6961 gcggcacggg cgaggtcggc ggtttcatcc accactaccg gcgggttgcc agcgcctgcg
     7021 gcaatcagac gtttattggt gtgtttacgc gccgcttcta ctaccgcttc gccgccggtc
     7081 accaccagaa ggccgatgcc cggaaacttg aacaaacgct gcgcggtttc gatatccgga
     7141 tttgccacag taaccagtaa gttttccggc ccgcctgcgg caacaatcgc ctggttgagc
     7201 agcgtgatcg cccgctggga gacttttttc gccgccggat gcggggcaaa aatgacgctg
     7261 ttgcccgcgg caatcaggct gatggcgtta ttaattacgg ttgccgccgg gttagtggac
     7321 ggagttaccg aagccaccac gccccagggc gcgttttcaa ttaaagtcag gccgttgtcg
     7381 ccggtcagca cctgcggaga gagacactca acgcctggcg tgccgcgcgc ctgagcgacg
     7441 tttttggcaa atttatcttc aacgcggccc atgccggttt cactgacggc aagttccgct
     7501 aaatctctgg cgtgtttttc gcctgcctca cgaatggcag caatggctaa ctggcgcatt
     7561 gccacgcttt ttaacccttg ctgggcgact ttggctgccg caacggcgtc atccagggac
     7621 gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt cactgctttg cattttcagc
     7681 agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg ccttatttgt
     7741 ggaaaattac ctgaccgcca gacaccacct catcgacaat gccaatcacg cacagatcga
     7801 ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcacc agtaacaccc
     7861 actccccggt tcccgcgcca atattgtcga tggcgacggc gcattgcccg tcaggattac
     7921 cttgtggatc aatcatttcc accatcagca atttgtcatg cgccagtccg tgatggcgta
     7981 cggtacaaac aatttgtcca gtgacgactg ccagtttcat acccgcctcc gtggcgtatt
     8041 tcaggtaaaa gctcccccta ccctccgcag aaggtaaaat gaaaaaggag agagcgtgac
     8101 gcccgaatcg acgtcacaca gggtgattac aggttgctgc tatcgccttt caggccgatc
     8161 gggaagactt cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga taccagctcg
     8221 ccgatacgct gtgccgctgc ggcacctgca tcggtcgcgg ctttacaggc tgcaacatca
     8281 ccacgaacca tggcagtaca caggccgccg ccaatctgct taacaccaac cagcttgacg
     8341 cgcgcagctt tcaccatcgc gtcagaagcc tcaatcagtg caaccaggcc ccgggtttcg
     8401 atcattccta atgcttccat tgtgctttcc tctttatcag ggtccagaac gggaccgttc
     8461 attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc ggcacagctg
     8521 ccaccagcgc cagctcgata atttcctgca cgctacaacc acgagagaga tcgtgcatcg
     8581 gcacggcaag tccttgtatc agtggcccga cggcacgata tccgccgagt cgttgtgcga
     8641 tgttgtaacc aatatttccg gcttccagcg acggaaaaac catcacattg gccttgccct
     8701 gtagcgggct ggcaggcgct ttttgcgccg ccacttccgg cacgaaggcg gcgtcaaact
     8761 gtaactcgcc atccaccacc agctttggtg cgcgctcacg gacgatttct gtcgcctgct
     8821 ggacgttagc aacacagggg tgacgggcgc taccattgct ggaaaacgac agcatcgcca
     8881 cgcgcggctc ttctccggtg atggcgcgcc aggtttcggc actggcaagc gcgatatccg
     8941 ccagctgcgc cgccgtcggc tgtggcacca cgctgcaatc ggcaaagccc aacgccgggc
     9001 cgctgtactg tggcaacatc aggaaaatga aagagagcgt tttacagcct ggctgcaagc
     9061 cgataatgcg taatccggca cgcagcacat ttgccgtgga agagaggttg cccgcgatac
     9121 agacatccgc tttaccggcg ctgaccattg ctgcggcgaa catcagaggg tcggtaagtt
     9181 tttccagcgc atccggcggc gttttttcgc ccgcgcgggc cagccagcga tgagcaaatt
     9241 cttcccgcat tgcgaggttg ccatgcggat caatcacctg tagcccgtcc atcgccacgc
     9301 cgtgactgag cgcaaactga cgaagttcaa acggattggc gaccagaatg ggcgttgcca
     9361 gaccttgttg atgtaaatat tgcgcggctt tcagcacacg ttggtctaac gcatccggaa
     9421 aaaccactct ggcgggcgct cgcaacgcca gttcacgaca acgttcaata atcattgcgt
     9481 tcccccagtc gttgttggat ctgcttcacc gttagcggct gcttcgttac gctcaggatc
     9541 atcatcacgt agaccgtgct ggaaaggcgg ttcagcgcct gcaaaatgtc cgggcgtaat
     9601 acttcaaagc tgcgggtgat aaagacctgc gccgctacgg tttcggtttc ccgcactttg
     9661 gtgcgtagca gattgagcaa cgcagcatcg cgcccgtggc tggcttccgg caccagatga
     9721 tcgtggtcga gatagcgcag cggctgatga gaaagccgat gcagatcttc gtcagaaagc
     9781 ccgacaatcg cctggcagcc cagcggttcc cccagcgcat cggcgcacat aatgttgcca
     9841 agacgcgaac ggatatccgc cagccacggc tgccacggtt ccgccagttc aatttgcagc
     9901 cacactgcca gcgcaatggt gctgtcgaga accgcgcgaa aacccagacg cggatcgctt
     9961 ttggcgacca ttttttccgc cgacaggtga gtcagcgtat ccggcttttt tgccaccggc
    10021 tggcggcaca gttcgcagca cgcctgcgga tgttcatcgc tactggtcag cccatgaacg
    10081 ggctgcggct gctgctgttc atcgtcaaca aacaggcggc cctgctcgtc aataaacttg
    10141 atgcgcagat ggcggctttc cagcaactcc cgggcagagg gcgtcaggcg actgtccgca
    10201 gggagatgga tctctgcgcc ttcgctgagc gtatggttcg ctcttagcca tgcttcggtg
    10261 atgaaatctt tcatagggat tgccagttag ccggccaggc gacatacagg aatttcacgc
    10321 tggatgtcat accaaattcg atgctggagc ctttcgggat aaacatcacg tcgcctgctt
    10381 tggcgatcat ggtttggcct tcgtggcgaa catgcagctc cccttccagc accatgtcga
    10441 tttcgtcgta gttcagggtc cacgggaaga atgcgttctc ccactgcata aaaccggcgg
    10501 ccatgctgct gccgtcgtct ccggtcacaa gatcagtcaa gcccacgcaa tgcggctctg
    10561 cgccgtcgaa acgaccaaac ttgacgctgc tgccatcaat cactttgatg ccgcctttgc
    10621 cggtcaccga tttgaagctc ggctgcattg cgccctgctc cagcgactgt ttttccttca
    10681 tcactttttc catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg
    10741 caatgatggt ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca
    10801 cggacgccgt taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg
    10861 ccacttcacg ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt
    10921 cgccgcgcgc gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc
    10981 tcctgtttgg tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg
    11041 ctgtttagct cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg
    11101 gcgacgtcag catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg
    11161 ccaatatcca gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag
    11221 atcagcatgt ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta
    11281 aaatattcac ccggcgtatc aatatcgccc ttatcattaa attccacggc ctgtgttttt
    11341 ctggcgaggg tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg
    11401 ctacccacaa acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa
    11461 agcgtataat ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact
    11521 gcgcccaccg agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc
    11581 acatcagcgg ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc
    11641 gtcataatgc caattgcgcc cgcatccgga acgccgattt tcttcgccag ttcctcaccg
    11701 ggatgcgcga tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg
    11761 cgttctttat ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg
    11821 tcagagtaaa ttaacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcactaagaa
    11881 tggagagata ttcgctgtgg tgcataaact ttcatatgca acgcatgaat ataaataaaa
    11941 taatatcgag atattatata gactgctttt tttattattc gcaaactcag taataagctt
    12001 tactaatata ttgcagccaa accgcttacc ctgaatattc agggtaagcg tgagagttaa
    12061 aaaagaatta cagcggttgg gtttacgctt caaccacggc cagcgccacc atgttgacga
    12121 tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg cccatcagca
    12181 ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa ctaatgcggg
    12241 cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg ctgtccggca
    12301 tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca
    12361 tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg
    12421 ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggc tcaataccaa
    12481 aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct gcgtctggtt
    12541 cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc agcgcgttca
    12601 ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acgctaaaat
    12661 gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca cgctgaacca
    12721 tgatcgcgcc gatcactgtc gggttactga tcagcgcccg ctgcgcctgt tcctgagtga
    12781 caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg
    12841 attcgttatt gacgatctca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa
    12901 tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta
    12961 ccagttcctg agtggcatgc agaacgcgcg cctcttcccc ttctggcaga acaacgcgct
    13021 tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga
    13081 actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc gccacgcccg
    13141 actccatcgc ggctttagcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg
    13201 gttttggaat gatgtattcc ggaccaaagc tcagatcctg atcgccatac gctgaagcca
    13261 ccacttcgct ctgttccgca tgggcgagtt ctgcaatcgc acgtaccgcc gccagtttca
    13321 tctcttcgtt gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg aacgggaagc
    13381 acaggacgtt gttcacctgg ttcggatagt cagaacgacc ggtgcaaatg atggcatccg
    13441 gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga
    13501 tcattggcgc acgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac
    13561 atccgaggaa aatatccgcg ccttcaatca catcatcgag ggtacgtttg ccgtcatcca
    13621 ccaccgcata tgcggctttg gtttccgcca tgtttggctc acggccctga tagataacgc
    13681 cttttgaatc gcaaaccacg atgttatgtt tttgcagacc cagcgctacc agcaggttca
    13741 tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt
    13801 tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc gtgccgtgct
    13861 gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac
    13921 attctggcgc tttaatgtct tcgaggttga ttccgccgaa ggtcggttcg agcgcagcga
    13981 caacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat acatcaatcc
    14041 cggcgaattt cttaaacaga acgcccttgc cttccatcac cggtttgcca gccagcgcgc
    14101 caatgttgcc taaccccagc accgccgtac cgttagagat caccgccacc aggttaccgc
    14161 gcgcggtata tttgtaggct tttaacgggt ctttttcgat ttcaagacaa ggtgcggcaa
    14221 cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg gttggagaaa
    14281 cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt
    14341 catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc
    14401 ctgcccacaa cgtggcgcac attattaccc cgccggagtc tacagacttt gagcaagtcc
    14461 aaactctcac cattaatata atgttttggt aataatccta taacactgat gttacctgct
    14521 taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc
    14581 agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa
    14641 cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagcggcg atattgagtc
    14701 cattcgccat tatcatcccc aggatgccac caccaatcct tcgctgttgc tcaaggctgc
    14761 cggattatca caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg
    14821 caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtta atttcggtgc
    14881 tgaaattctc aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac gcctctcttt
    14941 tgataaagaa aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg
    15001 cgttgagaaa tcacgcattc tgatcaaact ggcttcgacc tgggaaggaa ttagcgcggc
    15061 agaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttgccca
    15121 ggcgcgggcc tgtgcggaag caggcgtttt tctgatttcg ccgtttgtcg ggcgtattta
    15181 tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt
    15241 taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat
    15301 gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac
    15361 tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tcgccagtgg tacgtaaatt
    15421 aatcccacct tctcagacgt tcccacgccc agctcccatg agcgaagcgg agttccgttg
    15481 ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc
    15541 cgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt
    15601 gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgcggtac
    15661 aaaaagccaa ctctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc
    15721 tgtggaacga ttttcttaaa cataacccta ccgacccaac ctggtatgat cgcgaccgct
    15781 ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctgcat ctgaccggtt
    15841 acgacctgcc gctggaagag ctgaagaact tccgtcagtt gcattcgaaa accccaggcc
    15901 acccggagat tggttatacg cccggtgttg aaaccaccac cggtccactt ggacaaggtt
    15961 tggcgaacgc cgtcggactg gcgatagcag agcgtacact ggcggcgcag tttaatcagc
    16021 cggatcatga aattgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg
    16081 aaggtatttc ccacgaagtc tgttcgctgg cgggcacgct aggactgggc aagctgattg
    16141 gtttttacga tcacaacggt atttcgattg atggagaaac cgaaggctgg tttaccgacg
    16201 atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg
    16261 atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc
    16321 tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt aaggaagagg
    16381 cgcacggcgc accactgggg gaagaagaag tggcgctggc acggcaaaaa ctgggctggc
    16441 accatccgcc atttgagatc cctaaagaga tttatcacgc ctgggatgcc cgtgaaaaag
    16501 gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc
    16561 aactggcaga agagtttacc cgacggatga gcggtggttt accgaaggac tgggagaaaa
    16621 cgactcagaa atatgtcaat gagttacagg caaatccggc gaaaatcgct acccgtaagg
    16681 cttcgcaaaa tacgctt
//
LOCUS       DAJTMK010000085        16557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTMK010000085 DAJTMK010000000
VERSION     DAJTMK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16557
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.086"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..57
                     /locus_tag="OO135_002358"
     CDS             <1..57
                     /locus_tag="OO135_002358"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398183.1"
                     /translation="VELHDKVIGHYLNIKHYQ"
     gene            198..1475
                     /locus_tag="OO135_002359"
     CDS             198..1475
                     /locus_tag="OO135_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398184.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPVMGFFIDTVGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATH"
     gene            1745..2539
                     /locus_tag="OO135_002360"
     CDS             1745..2539
                     /locus_tag="OO135_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5398185.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFEWNHEATLDNIHAQKY
                     NKAYAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2610..3059)
                     /gene="rplI"
                     /locus_tag="OO135_002361"
     CDS             complement(2610..3059)
                     /gene="rplI"
                     /locus_tag="OO135_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5398186.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(3101..3328)
                     /gene="rpsR"
                     /locus_tag="OO135_002362"
     CDS             complement(3101..3328)
                     /gene="rpsR"
                     /locus_tag="OO135_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5398187.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3333..3647)
                     /gene="priB"
                     /locus_tag="OO135_002363"
     CDS             complement(3333..3647)
                     /gene="priB"
                     /locus_tag="OO135_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5398188.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3654..4049)
                     /gene="rpsF"
                     /locus_tag="OO135_002364"
     CDS             complement(3654..4049)
                     /gene="rpsF"
                     /locus_tag="OO135_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5398189.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4376..4651
                     /locus_tag="OO135_002365"
     CDS             4376..4651
                     /locus_tag="OO135_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5398190.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4726..5277)
                     /locus_tag="OO135_002366"
     CDS             complement(4726..5277)
                     /locus_tag="OO135_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR5398191.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5374..6060)
                     /gene="ulaF"
                     /locus_tag="OO135_002367"
     CDS             complement(5374..6060)
                     /gene="ulaF"
                     /locus_tag="OO135_002367"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5398192.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYWQK"
     gene            complement(6060..6896)
                     /gene="ulaE"
                     /locus_tag="OO135_002368"
                     /pseudo
     CDS             complement(6060..6896)
                     /gene="ulaE"
                     /locus_tag="OO135_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            complement(6906..7556)
                     /gene="ulaD"
                     /locus_tag="OO135_002369"
     CDS             complement(6906..7556)
                     /gene="ulaD"
                     /locus_tag="OO135_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5398193.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(7570..8034)
                     /gene="ulaC"
                     /locus_tag="OO135_002370"
     CDS             complement(7570..8034)
                     /gene="ulaC"
                     /locus_tag="OO135_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5398194.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(8044..8349)
                     /gene="ulaB"
                     /locus_tag="OO135_002371"
     CDS             complement(8044..8349)
                     /gene="ulaB"
                     /locus_tag="OO135_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5398195.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(8365..9812)
                     /gene="ulaA"
                     /locus_tag="OO135_002372"
                     /pseudo
     CDS             complement(8365..9812)
                     /gene="ulaA"
                     /locus_tag="OO135_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            10117..11181
                     /gene="ulaG"
                     /locus_tag="OO135_002373"
     CDS             10117..11181
                     /gene="ulaG"
                     /locus_tag="OO135_002373"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5398196.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTIPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNLEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            11289..12044
                     /gene="ulaR"
                     /locus_tag="OO135_002374"
     CDS             11289..12044
                     /gene="ulaR"
                     /locus_tag="OO135_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5398197.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(12041..12790)
                     /gene="yjfP"
                     /locus_tag="OO135_002375"
     CDS             complement(12041..12790)
                     /gene="yjfP"
                     /locus_tag="OO135_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5398198.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDIVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12972..13301
                     /gene="bsmA"
                     /locus_tag="OO135_002376"
     CDS             12972..13301
                     /gene="bsmA"
                     /locus_tag="OO135_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5398199.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQSTPQPALPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            13450..13725
                     /gene="yjfN"
                     /locus_tag="OO135_002377"
     CDS             13450..13725
                     /gene="yjfN"
                     /locus_tag="OO135_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5398200.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13842..15467)
                     /locus_tag="OO135_002378"
     CDS             complement(13842..15467)
                     /locus_tag="OO135_002378"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5398201.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(15551..>16557)
                     /locus_tag="OO135_002379"
     CDS             complement(15551..>16557)
                     /locus_tag="OO135_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5398202.1"
                     /translation="NGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMCLE
                     VVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAI
                     QERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGV
                     GGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLL
                     WRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDY
                     ADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG
                     "
BASE COUNT         4236 a         4348 c         3975 g         3998 t
ORIGIN      
        1 gtggagctgc atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt
       61 ggagtcatta ccgacttttt aaagggagaa atttacacac aataattttc aatatttcct
      121 ctgtacctga tttttattac ctattacacg gaaataccaa caaccagggt ttattaatat
      181 aattaaaagg tttaaacatg aataaaataa agaattaccg gtggcatatg attgccctcg
      241 tatgctttat cactgtaatc aattatctgg acagaacggc gttaggtatt gcggctccaa
      301 cgattatgga gacaaccgga ataactaaag agcaatattc atggattgtc agtgcattcc
      361 agttggccta tacattaggg caaccggtaa tgggcttctt tattgatacc gtgggtctga
      421 agttaagttt tgcgatatgt gccgcaattt ggggcctggc gacaatgggc catgcactaa
      481 ccggaacgtg gtctggtctg gcatttatgc gcgccctgat gggtttcagc gaagcgtcgg
      541 caattccggc gggtgtaaaa accgcatcaa catggttccc ggcaaaagag cgtggcgtgg
      601 cgacaggtgt tttcaatatg ggcacctcac tcggcgcgat gcttgctcca ccgttgattg
      661 cctggtgcat tatgtttcat agctggcaat ttgcgtttat tgtttcaggt agtcttgctt
      721 tgctcgcggc tttattttgg ttcttttgtt ataaagatcc gaaagatgcc aaacgccttt
      781 ctgatgaaga gcgccactat attgaatcag ggcaagaaca gcatcttaaa acagataaga
      841 aagaaaaaac gtcaatcaag catatcctca gccaacgtaa tttctggggg attggcatcg
      901 cgcgttttct cgcagacccg gcatggggaa ccattaactt ctgggtgccg attttcttcg
      961 tcgaaacgct gcattttagc ctgaaagaaa ttgccatgtt cgtctggctg cctttcctgc
     1021 tgggcgatct cggctgttta gccagtggtt ttgtcgcgaa gttcttccac gatcgcggcg
     1081 tgagtttaat taactcacga agaattacct tcactattgc agccgtcatt atgatgacga
     1141 ttggcctggt gagtattgtc gaaaatccct acattgccgt attactgatt agtattggcg
     1201 cgttctcgca tcaatgtctt tctactgtag cagcaactct gggtggcgac ctgttcaaaa
     1261 aagacgaagt agctaccgca gtgggtatgg caggagcctg tgcgtggagc ggtcagttga
     1321 ttttcaacct gttcatcggg gcattcgttc acattatcgg cttcgcgccg ttctttattg
     1381 ccctggcttt ctttgacatt attggtgcta ttgcgttgtg gacgcttatc aaagttaaag
     1441 atgaagaacc acaagtacag ttagctaccc actaattatt ttaaataaaa acgctataat
     1501 tgaggttatg gcgtttttaa agaagcttga gttatcgaac ctcgcttacc aacgcgttgt
     1561 cagtcatctc ccccctccct agtgcatcgt tatatccgtg atgagcgatt tttgatatta
     1621 atatctttca ccatccgcta ttttcaccac ttcagaaaac aaacttattt ttattaatat
     1681 caattctttt tctttgaaca ttcaactgcg atatttgtaa aaacattaaa caggcaacaa
     1741 taaaatgaca ttaccaacaa ctatttattc attccccgca tatttacctc gtttttcgtc
     1801 caccgacaaa ccagcaaaat taaaatttca tcaatacgca cgcgctacat tactgtcaaa
     1861 tcgtggaaga gatcataatt gcgatggcag acgtactgtt gagatccaca aactagatct
     1921 ctctgactgg caagcattca ataagttagc aacgaagtgt aatgcttacg atggagtaac
     1981 aatgaatggt gataattctt ttgaatggaa ccatgaagct acattagata atattcacgc
     2041 ccagaaatac aataaagctt acgcgggtgc gaggttaacc gcagaactga aatatttatt
     2101 gcaagatgtc gaatccttcg aacccaacag taaatacaca atacacgaag tggttttggg
     2161 acctggttat ggtactccag actataccgg ccaaacgata ggatacgttg taacccttcc
     2221 tgcccaaatg cctaattgct ggtcatctga actaccaaca atcgatcttt atatcgacca
     2281 acttcgcact gttacaggcg tttctaatgc cctcggattc atcattgctg cattgctaaa
     2341 cgcgtattct gatttacccc atgatttgaa aattggtctg cgttccttat caaattcagc
     2401 agcaatctac tcgggtttag gtttcgagcg agtgccgcag gaccgagaca taaggagtga
     2461 tcgtatgcat ctaacgccag caaaccatcc tgacttatgg acacaagaga atggtgaatg
     2521 gatctacttg cgaaactaat acctgacgaa aaatagaaag caaaacgccg accaatggtc
     2581 ggcgttttta cgtctcgttg aataacgaat tattcagcta ctacgtttac gatcactttc
     2641 gcgaatactt cgctgtgaac ctggaagctc acttcgtgtt cgccagtggt acgcagaacg
     2701 ccgttcggca gacgaacttc gctcttagcc acttcaacgc cagctgcagt tacagcgtca
     2761 gcgatgtcgc gagtaccgat ggaaccgaac agtttacctt cgtcgccagc tttagacgcg
     2821 atggtaacag tttccagtgc attgattttc tcagcgcgag cattagcagc tgccagaact
     2881 tcagccagtt tagcttccag ttcagcgcga cgtgcttcga agaattcaat gtttttcttg
     2941 gtagctggaa cagctttacc ctgcggtacc aggaagttac gagcatagcc cgctttaacg
     3001 tttacctgat cacctaggct acccaggttt gctactttat caagcagaat aacttgcatt
     3061 accttatcct ctcaaagtcg tattaatgga ccgtgaccga ttactgatgg cgatcagtgt
     3121 acggcagcag ggacaggtag cgagcgcgtt tgatagcgcg agccagctga cgctggtatt
     3181 ttgcacgggt accggtgata cggcttggga caatcttacc gctttcggtg atgtagtttt
     3241 tcagcgtagc gatatcttta tagtcgatct cttgaacgcc ttccgcggtg aaacggcaga
     3301 acttgcgacg acggaaataa cgtgccatat ggctagtctc cagaatctat caattcaatc
     3361 tgctcggcat gcaaaaccat tttgctcagt ccgttctttg ccttgtggca tgaaatgaac
     3421 ccctgaacgg ttatgcgact gccgaccgtt atactgtgag taatggcctg gttttcgtgt
     3481 ccgctaacaa taacgggcat ttggcaccac gcctgccggt gaaaaccggc ttcctcctgc
     3541 acagaacgat gctcaagcac gaactggcag tgaggaattc ctgatggact gacctttcga
     3601 aggggagtcc tgcacacggt gccggacaac accagacggt tggtcatcag aaattactct
     3661 tcagaatccc cagcttcagc atcatcagcg gtttcgtttg cgaaatcatc gcgacgctca
     3721 cggcgctcgt ctttcgcttt aaccatcgga gatgcttcgg taacagcgtg cttggtacgc
     3781 ataaccatgc tgcggataac ggcatcgttg aagcggaagg tagtttccag ctcatcgatc
     3841 acttcctgcg gagcttcaac gttcatcaga acgtagtgtg ctttgtgcag tttgttgatc
     3901 gggtaagcca gctgacggcg gccccagtct tccagacggt ggatcttgcc ttctgcacca
     3961 gtgatggcag cagtgtagcg ctcgatcatg cccggaacct gttcgctctg gtcaggatgg
     4021 accataaaaa cgatttcgta atgacgcatc gaattgctcc ttacggatta ttcagcctcc
     4081 tgtctgggtc agccgaatcc cggggaggca aggaacgtgt taaaggtcgg ctgaaaaatg
     4141 acgcgttata gtacttgtat gccccactaa actcaaggcc agccgacaaa taaatctcat
     4201 ctttcgctca aaagaactaa ctcgctgatt tgaagttcat cacattccgt cacagtgttt
     4261 tttcgaacaa ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa
     4321 atcagaagat catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt
     4381 cagtcgcgtt ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat
     4441 tgaaattaat aaccaccagg ccagaaatat ggacgacgta cagagcttag gcgtgattta
     4501 tatcaatcat aatttcgcca ctgaaagcga agcacgtcag gcattaaatg aagagacaga
     4561 tgcgcagggc gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa
     4621 tatgcacgcc agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca
     4681 tagtttgctt tgcccccttc gcagggggct tttttatacg gttattcacc ggattgcgac
     4741 ttccggatct ggctcatgag taatgcgatt acgtaagtcg cggcgcataa tttctattat
     4801 ccagaaccag aaaatatgcc ccaggatttc agaggcatat tcgtcaaaag gcaattgagt
     4861 gagcggcggc gttaaattca gcgccgggca aaatataccg tggactgcca ctgtcacaat
     4921 aagccctgct aatatcccct gccataattt cactttcggg aaaatttccg cgacaataca
     4981 ataccctatc gcaaaaacca gtgagaagat gatatgcgtc accatcaccg ggttgataat
     5041 atgttccgag aatgtataca cagtttgggt tgggtcgata cctaaataat cccgtagaaa
     5101 gatatacggc gggttaaatt catcgcgacc accggaaaat gtgcgcggtg gcaaggggac
     5161 ttccgctccc catttaacaa atgctgaaat aattcctgaa agtacaccca caagtaacgc
     5221 aacgccatag cggcgacgtt gcggatccgt tctgacaaac aatccattga tagccataaa
     5281 aacacctgtt gtttatacga ttttttaact gaagcggccc cggaatcaga ccaattacat
     5341 cccgcaggag gtaataattc cggggcgcac tgcttacttc tgccagtaat aggcgttagg
     5401 accgtgctta cgcatgaagt gtttattcat gaggaagctg tcgatgtgat tcagttgtgg
     5461 gttaatactg cgcgcaatcc acgccatttt cgccacttct tccatcacca ccgcgttatg
     5521 caccgcgtcg tgggcatctt tcccccaggc gaacggcccg tgctgataca ccacaattcc
     5581 cggcgtatgc agcggctcgg cgtcacccag cgtttcgata atcactttgc cggtgttcag
     5641 ttcatactcg ccctgcacct cttcttcgct taatccacgc gtacacggga tgtcgccaaa
     5701 gaagtagtcg gcgtgcgtgg tgcctaacgc cgggatcgcc agccccgcct gcgcccatgc
     5761 ggtggcatga gttgaatggg tatggacaat gccgccgagc gacgggtaac gacggtagag
     5821 ttcaagatgc gtcgcggtgt cagaagacgg gcgatacttc ccttctacca ccttgccgct
     5881 catatcgacc accaccatat cgtctgcttt catggtttcg taggcaacgc cgctgggctt
     5941 gatcaccacc agcccgcgtt cgcggtcgat agcgctgacg ttgccccagg taaaggtcac
     6001 cagcccatag cgcggcagat ccatgttggc ttcaaacacc tgctgtttta gcttttgcat
     6061 tatgccgcct ccaccatgcc tgctttggcc atgcgcgctt tcaccacttc tgccgccggg
     6121 tcttccgccg tttcgctcca catctcaatc aggtacggcc cgcaatagcc actctgtttg
     6181 agcgtttcga agcaacgttc gaaatccacc acaccttcgc caaatggcac gtttttgaag
     6241 acgcccggtt tggtgtcttt cacatgcacc gcgacgatat gcccgattcc ggcctgcaac
     6301 tccatctgca catcgttgtc ccacgccgac aggttgccga tatctgggta gagctggaac
     6361 cacgggttgt tgagatagtg cgcgtaaccc agcgccttgc tgatggagtt catcaacgga
     6421 taatccatga tctccatcgc cagcgtcacc tgcgcgcggc tcgccatctc aacgctctct
     6481 ttcaggccgt cacggaaacg acgacgcgtt tcgttattgg cttcctgata gtaaacgtca
     6541 tagcccgcca gctggatcac gcgaatgcca acgtcctagg cgaactggat ggctttacgc
     6601 ataatctcca gcccctgcgc ccgcaccgcg tcatcttcgc tgcccaacgg gaaacgacga
     6661 tgggcagaaa ggcacatgga cggcacacgc acgccggttt caacaatcgc attcaccagc
     6721 gccagacgct gctcgcggct ccagtcgagg cgcgacaggc gctcgtcagt ttcatctacc
     6781 gacatctcga caaaatcgaa gcccaacgtt tttgccagtc gcaggcgttc cagccagcac
     6841 tccccggcgg ggagcgcttt ttcatagatg ccaagcggga tttgtttgga caacatatcc
     6901 gctccttagc cccacagttc ggcgatggaa cgtttgaact gacgcgcggc ttcaaccgga
     6961 gaagcggcat cacggatgct gcgaccggcg ataaacacat gaattggaat gcctttgaac
     7021 agcggcagat cttccagcgc caggccaccg gtgacggtga ctttgaagcc catatcggaa
     7081 agacgtttga tcgcagtgat atcggcttca ccccacgcca cgccagcggc ctgcgcgtca
     7141 cggctgcggt gataaaccac ctgctgaata cctgcgtcgc gccactgttg cgcctgttcc
     7201 caggtccagt aaccggtcag ttcgatctgc acgtcgccgt taaactcttt tgctacgtcc
     7261 agcgcacctt tggcggtgtt gatatccgca caacaaatca cggtcaccca gtcagcgttg
     7321 gcttcgaagc acatacgaga aagaattttg cccgcatcgg caattttggc gtctgccagc
     7381 acgattttgt gcgggtagag cgctttcaga tcgcgaaccg cacgcacgcc ttcgcccacg
     7441 cacagaatgg tacccacttc gataatgtcg acttcttcgg caatcaggcg agtggtttcg
     7501 taggcgctat ccatagtctg gttgtccagc gcgacttgca acatcggtaa tgacatcttc
     7561 aattccttct taagctgccg cgttggtgcg gtcaatgaga tccagtactt cctgctcggt
     7621 acggcaggca cgtaaacggt cgaaattttc ttcatcttca aacaggttga cgatctgcat
     7681 gatgcccact tcctggtgcg tgttggcatc gaccgccgcc atggtgatga ggatatccac
     7741 cgggtcgtta tcttcgtggt tgaactccag cggctttttc agcgtcacca gcgcaaaacc
     7801 ggttttctta acgccctctt ccgggcgacc gtgcggcatt gccaggcctg gtgcaagcac
     7861 aaagtacgga ccgtgctgtt caaccgcatc cagaatcgcc tggtagtaac gcggctcgac
     7921 aacatccgcc gccaccagca ggtcaacgcc gatttttact gcgtcctgcc atgtctcagc
     7981 ttcagcctgc aggcggatgg atttattctc cgccagcgaa tcacgtaatt tcatggcgcg
     8041 tccttacttc acatcctgcg ggaaatgcgc tttgatcact tccagcagtt tcgggccaaa
     8101 gtcggcagga gagagcatgt tgcgcacgcc aaccacgtat ttgttgccgg tcacggtgat
     8161 ttcgcccgca atgtgcgtag aagcgatgat gatatccgcg ccgctcaact cgcttttgta
     8221 ttcgccaacc gcgcagctat ttaccgtgtg gtcaatgttt gattgggtta aaaactggtc
     8281 cactttcatc ttcatgatca tggaactgcc ttgtccgttg ccacacacag ccagaatacg
     8341 tacggtcata atcaaaactc cttattaagc agactgttct gccagttgtt tttctgcatc
     8401 ttcttctgcg cgcagtgcgc ggccagcgaa gaacatataa gcagtgcaat tacaatgatg
     8461 acggccataa aggcgatacc gatggagaag aagccctgca tcatcggcgg tgccagaatc
     8521 gaccagtccg ccatgcccat ccaggcgctc ataccggtga gtttcaccgc ccagacgcag
     8581 ccaaagattt caatcatccc catcaccaga catatcttca gcgccgcacg ccagccgccg
     8641 aagtggttag cgaacacgcc gatggtggcg ttggagaaga acatcgggat aaagccggga
     8701 ataatcagga tcgaggaacc gcaggcgacc aggatgccaa ccgcaatcag ctgaccgatg
     8761 gtgccccaca taaagcccca aaccacggcg ttcggcgcga agctatagat agccgcacag
     8821 tcaatcgcca gaaccgcacc agggatcagg cgctgggaga taccgttaaa tgcttcagag
     8881 agttccgcca caaacatgcg cacgccctga gtgatgatga agatcgccac cgcgaaggag
     8941 aaaccagttt gcaggatgta caccgtccag ttcactttgc ctgccatcgc ctgcacggtg
     9001 tcgataccga aggagagcag aatggcacca aagaagatgg tcatcacaat cgccgtggag
     9061 acgatgttgt cgtggaaaat attcagccag cctggcagtt tgaggtcttc aacgctttcc
     9121 tcttttttgc cgaggaacgg cgcaacttta taggcaatcc atgatgcaaa ctgttgctgg
     9181 tgaccgatgg agaaaccgca gccgtccgtc acttcctgag tcggcttgta catcatgttg
     9241 gaggtaatgc cccagtagag cgaaaccaga atcgccgtgc agataatggt tgtccacatg
     9301 gagtagccga aaatgaacaa cgtaacggca atcagccccg cctgctggaa catgatatga
     9361 ccagtcagca tgatggtacg aataccggta atgcgacgca gcagcacgta acagatgttc
     9421 agcgccagcg ctaacaacac cgcgtaacct acccagctat aggcatcgcc catgcggtcg
     9481 atggttgcca tcatcgacgc gtaagtatcg gaaattgcgc cgttaatgcc gtagacttcg
     9541 gacattttcg ccaccaccgg tttgaaggtg ctggtgagga tgccggaccc tgcctgcaac
     9601 aacatgaaac caattatggt tttaatcgtg cctttaataa taacgctgac acttttgcgc
     9661 agtaggatgt agcccagaca ggtcacaata cccagcaaca acggggcatt ggtcatgacc
     9721 tggttaaaaa acacggtaaa gatgttgtag agacgatctc ctgaagaaga ggtaaccggg
     9781 tattccgcac acccggaatg ttgtgcactc actctaattt tcaaaagtaa tcacaacaag
     9841 attattttat gattaaatgt gacgcgaccc gcaaataatt ccatacggat agagtgggat
     9901 tcacacaacg aaacaattat ttatttaaat ttatttaaat tcatttagtt aatttaacta
     9961 cacattccat taaatctttc cacactcaaa atcaacaaca acttccctgg caaaaagtca
    10021 aaaaatggtc attgccttcc tgaaaaaata gtgtcaggat taatcaaaac caatcacata
    10081 ttgattcgaa ttgaactatc atgaaggtaa atggcgatga gtaaagtgaa aagtatcacc
    10141 cgagaatcct ggatcctgag cactttcccg gagtggggta gctggctgaa tgaagaaatt
    10201 gaacaagagc aggtcgcccc tggcacattt gcgatgtggt ggctaggctg caccgggatc
    10261 tggttgaaat cagaaggtgg caccaacgtt tgcgttgatt tctggtgcgg cactggcaaa
    10321 caaagtcacg gtaacccgtt aatgaaacag ggtcaccaga tgcagcgcat ggctggcgtg
    10381 aaaaaactgc agccaaacct acgcaccatc ccgtttgttc ttgatccgtt tgcgattcgc
    10441 cagatcgacg cggtactggc gactcacgat cacaacgatc atatcgacgt taacgtcgct
    10501 gctgccgtga tgcagaactg tgctgatgac gtaccattca ttggaccgaa aacctgcgtt
    10561 gatttgtgga ttggctgggg cgtaccgaaa gagcgttgca tcgtggtcaa accgggcgat
    10621 gtggtgaaag tgaaagacat tgaaattcat gcgcttgatg ctttcgaccg tactgcactg
    10681 atcaccctgc ctgccgatca aaaagcggct ggcgtactgc cggacggcat ggacgatcgc
    10741 gcggtgaact atctgttcaa aacgcctggc ggctccctgt atcacagcgg cgactcccac
    10801 tactctaact attatgcgaa gcacggtaac gaacatcaga tcgacgtggc tttaggttca
    10861 tacggcgaaa acccgcgcgg tatcaccgac aaaatgacca gcgccgatat gctgcgtatg
    10921 ggtgaagccc tgaatgcgaa agtggttatc ccgttccacc acgatatctg gtcaaacttc
    10981 caggccgctc cgcaagagat ccgcgtgctg tgggagatga aaaaagatcg tctgaagtat
    11041 ggcttcaagc cgtttatctg gcaggttggc ggcaaattta cctggccact ggataaagac
    11101 aacctcgagt accactatcc gcgcggtttc gatgattgct tcactattga accggatctg
    11161 ccgttcaagt cattcctgta atctgtcgtt tataccggat agcgtggaga agttcgcgct
    11221 atccggcgta gtatttcctg gctatttcaa atatcatcta aaaaaatcaa attttatcgg
    11281 aatagctcat gactgaagca caaagacatc aaatcctcct ggaaatgctc gcacaattgg
    11341 gctttgtgac cgttgagaaa gtcgttgagc gtctgggaat ttcgcctgcc actgcgcgac
    11401 gcgatatcaa taaacttgac gaaagcggca aactcaaaaa ggtgcgcaat ggcgcagaag
    11461 ctattaccca acagcgcccg cgctggacgc cgatgaatct gcatcaggcg cagaatcacg
    11521 atgaaaaagt acgtattgct aaagcggcct cgcagctggt taatccgggc gaaagcgtag
    11581 tcatcaactg cggctccacc gcgtttctgc ttgggcggga aatgtgtggt aagccagtgc
    11641 aaatcatcac taattatcta ccgctggcaa attacctgat cgatcaagaa catgacagcg
    11701 tgatcattat gggtggacag tacaacaaaa gtcagtccat cactttaagc ccgcagggca
    11761 gcgaaaacag tctctatgcc gggcactgga tgtttaccag cggaaaaggg ctgaccgcag
    11821 aagggttgta taaaaccgat atgctgacag caatggcaga gcagaagatg ctgagcgtgg
    11881 tagggaaact ggtggtactg gttgatagca gtaagattgg cgaacgcgcg ggaatgcttt
    11941 ttagccgtgc cgatcaaatc gatatgctta tcaccggcaa aaatgctaac ccggaaatcc
    12001 tgcaacaact ggaagcgcaa ggggtcagca ttctgcgtgt ttaaagatgc tggcggaaaa
    12061 atgtcacggc agcatctaac gcctcaggcg taatgcggtg acgcacgcct ggctgccatg
    12121 aacaggttag cagtttatcc cgtcccgtct cgcttaaggc ctgctgcaaa cgtagtgatt
    12181 cgtcggcagg cacaatgtca tcgtccaggc catgccacag cagtagaggt ctgtccccaa
    12241 gttgttccag gtggtttgtc gcttcccact ctgccagcgg cgcgataatg ttattgaatt
    12301 cattctgctg tgctgccgtt tcaggtatca gcggtggaaa cagtgaacgg gcgagcgatg
    12361 taaaatagcc cgatcccatc atgctggcgg tacatttcac cgtggggtgg cgagcggtaa
    12421 ttcccagtgc cgtcatcgcg cccatcgacg cgccaccgac tgccagacgg tcatcaagca
    12481 gccagttttc ttcggctatt gccgcacgta aagtagtgaa ttcctgcata ctttgtagca
    12541 agatttgcca gaattgattt aaccgccgcg cttcgtcgct actaaaacgg ctaccgtgat
    12601 cgggcgcatc cggcatgatc acccgcaaac cagcctgcgc cagcgcaacg gcaaaatagc
    12661 tatacaccag gctggatgaa gtaaagccgt gataaaaaat tacgcacggt aacggggtat
    12721 ctttttgccc gacaggataa gcatgaagaa cgggaatatc tgccagctcg cgtgattcta
    12781 tttcaatcat ctgtctctcc tttttgtctg gtatgacatg aagaaatcct tagcacaagt
    12841 ttctgctggc ggtattagtt cataatgttg agatgtgggt tacgctttcg tcacgttttc
    12901 attcgaaaat cgcgtactag gtaacaattc gggaacattc ccccaaagtt aaattaataa
    12961 ggcactacac tatagttagc aggaaacgta atagcgttat ttaccggttt gccagtttat
    13021 tattggtgtt gatgttaagt gcctgtagcg cactgcaaag tacgccacag ccagcactgc
    13081 cagtaacgaa ccatccgcaa gagattcgcc gcgaccagac gcaagggtta cagcgaatag
    13141 gtagcgtaag cacgatggtt cggggttctc cggatgacgc attagcagaa atcagagcaa
    13201 aagctgtcgc tgcaaaagct gattattacg ttgtcgtcat ggtggacgaa accatcgtga
    13261 caggacagtg gtattcacaa gccattttgt accgtaaata aatcagacaa cttacatagt
    13321 cagctttaca ttgctttgcg tccctatgcg tttacctgtg cacaataaat taccggctgg
    13381 aagcctgggc gtttattacc gcgatggaat gccctgcaac atgtggggaa acgaaaaatg
    13441 gagctgacga tgaaacaatt acttgcttca ccctcgctgc aattagtgac ttatcctgcg
    13501 agcgccacgg cacagtctgc agaattcgcc agtgctgact gcgtgacggg cctgaatgaa
    13561 ataggtcaga tctcagttag caatatttca ggggatccgc aggacgtgga acgtatcgtg
    13621 gcgttaaagg ccgatgaaca aggtgcgtca tggtaccgca ttattaccat gtacgaagac
    13681 cagcagcctg ataactggcg cgtacaggcg atactgtatg catgagtgtc atctacagca
    13741 aatgaacaat gccggattcg gcgcgagcgc cttgtccggc ctacaatttt cacatatttc
    13801 attgagttaa tcgaaaccag cgtcgcatca gtcgtatacg cttacacaca cactcccccc
    13861 gtcgcccgca gcaataaatc attctggatc tgctctgaca accgtacgcc gccgcgcgta
    13921 tctaacatca cctgacacca cgcctgcgcc attggcggag aagcatattt caacatttgc
    13981 gcaccgcagc ccagcaggaa tagctgatga gtaatctctc gccccagttc ttcagctggc
    14041 ttacgcagct gctgctgtaa acgacgaacc gcgcgatcaa aatagcgatc ctgccctttc
    14101 acttccacaa atgcttccga caataagtcg tatacgcccg cttgcttatt gagaacgcgc
    14161 aacacatcca ggcacataat attgccggaa ccttcccaaa tactgtttac cggcatctcc
    14221 cggtaaagcc gcggcagctc gctctcctcg caataaccaa tgccgcccag cacctccatc
    14281 gcttcggcca caaacggcat acctcttttg cagatcacaa attttgccgc aggcgtaaat
    14341 aaacgcgccc acagggcttc tttggcatcg gcacgccggt cccacgctcg cgcaagacga
    14401 aacagcaacg ccgtttgccc ttcaagctga agtgccatgc gacttaagac atgacacata
    14461 aggggctgtt ggatcaatgg attaccaaaa acatggcgtt gatgtgcatg ataaatcgcc
    14521 agcgaaaatg cacggcgcat catggcatgg ctacccaggg cgcaatcaaa acgcgtcatc
    14581 ccacccattt tcaggatcag acgaattcct tccccttcct gccccaacaa ccagccaatg
    14641 gcatcctgaa actccacttc gcaactggcg ttagagcgat tacccagctt atctttcagc
    14701 cgctcgaggc gaatcgcgtt gcgttgccca tcaggcaaaa agcgcggcac aaaaaagcag
    14761 gacaaaccgc ccgtagtctg cgccagcacc agatgcgcat cgctttgcgg caccgagaaa
    14821 aaccatttat gccccaccag ccgataagag ccatcttcca gacgctctgc acgggtggtg
    14881 ttgctcatga catcggaacc gccctgcttt tccgtcattc ccatgccaat caacaaaccg
    14941 cgtttttgcc cacctggcaa taagtgagaa tcgtagcgat cgctcagcag cggcgtggtc
    15001 cagtcctgaa acggcgtggg taacatctgt aacaacaatg gcgtggcggc aaaggtcatc
    15061 gttattggac ataacgaccc agcctcaacc tgcgcatgta acataaaacg cgccgcgcgc
    15121 gccacaaatg cgccggagcg agcgtcttct tcccaggcaa gattgtgcac ccgattggta
    15181 cataacgcct gcatcagcag gtgccaggcg gggtgaaaac gcacatcgtc cagacggcgt
    15241 ccttgcgcat cgtagcgcaa taattcaggc ggattcacat tcgccagccg ccccagttca
    15301 agagattcag ccgttcctaa ctgctgacca atactggcta gaaaatcgct atcccagcca
    15361 gcaccttcac gcgttaccgc ttcacagagc gcgccatcag acaggtataa gttgctgtta
    15421 tttaatggta taggttgatt aaaaacggtg tgagtttgcc agtgcactgt gtctccctcc
    15481 atcaatggca gtcaccctta gtatggtcac tgccatggat tcctgacaga aagagattgc
    15541 taaaacattc ttatccagca atgtaatgcg gaacgaaacg tgaggtgtct ttggtgatca
    15601 gcgtgttatc ttcacggatc cccattccgc acgcttcatc atcgacaatc cagctaccga
    15661 tgagtgtgta gctatcgcca aatcgcggca gaggttgaaa cgcctggtag atcatcggtt
    15721 catcggcgta atcaccatca gcgtggtcaa cgacgttatt ctgaccgtcg aaaatggtga
    15781 cgtttccgcc ttcgcgtgag tagatcggtt tacgcacata gctttcgcca gcggcaatct
    15841 gcggtttttc gccctcaaac caggatgcaa gaaggttagg atgacctgga aagaagcgcc
    15901 aaagcagcgg cattagccct ttattactca agatactttt ccataacggc tccacccatt
    15961 gctcgcgacg cttgcgcagc agcggaccgt tatcgtcacg catcatccac tccagcggat
    16021 acagcttaaa tgcacgctgg atgacattat catcaagatc ggtcagtacg ccgccgacgc
    16081 ccaaaccgag atcttcaatg tagataaacc gcgactcctg ccctgcctgc tgggcgcagt
    16141 cctgcaagta cagcacggta ctcctgtctt catcggtgtc ctgacagcag caaaaataaa
    16201 acggttcccg actgtaaagc tcactaaagc gcgaaatcag gcgttcctga atggcgttgt
    16261 actccagcag cttcaccggc gcattgccac accaggcaaa atccatccgg ccatacagcg
    16321 aaggatcgcg tgcgcgccag ctttcggcga tcacatccca gtacaacggc gggatcgcca
    16381 gttgcgtcag gatctcttcg tctttaaccg cgcgatccac cacctcaagg cacatctgat
    16441 gcagttccgc agtcggtttt tcgatctgct cttcaatctg gcgcagagta aaacggtaag
    16501 cccgactctc gtcccaatag atttcattgt cgatgatatg aaagtcgaaa ccgttgt
//
LOCUS       DAJTMK010000086        16535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTMK010000086 DAJTMK010000000
VERSION     DAJTMK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.087"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            193..2040
                     /gene="ldtD"
                     /locus_tag="OO135_002380"
     CDS             193..2040
                     /gene="ldtD"
                     /locus_tag="OO135_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5398203.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPTHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2221..2769
                     /locus_tag="OO135_002381"
     CDS             2221..2769
                     /locus_tag="OO135_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5398204.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2796..3443
                     /gene="gloC"
                     /locus_tag="OO135_002382"
     CDS             2796..3443
                     /gene="gloC"
                     /locus_tag="OO135_002382"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5398205.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO135_002383"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OO135_002383"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5398206.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO135_002384"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OO135_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5398207.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6731..8131)
                     /gene="asnS"
                     /locus_tag="OO135_002385"
     CDS             complement(6731..8131)
                     /gene="asnS"
                     /locus_tag="OO135_002385"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5398208.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO135_002386"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO135_002386"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5398209.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TTGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OO135_002387"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OO135_002387"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5398210.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12423..13174)
                     /gene="ssuB"
                     /locus_tag="OO135_002388"
                     /pseudo
     CDS             complement(12423..13174)
                     /gene="ssuB"
                     /locus_tag="OO135_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13171..13962)
                     /gene="ssuC"
                     /locus_tag="OO135_002389"
                     /pseudo
     CDS             complement(13171..13962)
                     /gene="ssuC"
                     /locus_tag="OO135_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13973..15118)
                     /gene="ssuD"
                     /locus_tag="OO135_002390"
                     /pseudo
     CDS             complement(13973..15118)
                     /gene="ssuD"
                     /locus_tag="OO135_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15115..16073)
                     /gene="ssuA"
                     /locus_tag="OO135_002391"
                     /pseudo
     CDS             complement(15115..16073)
                     /gene="ssuA"
                     /locus_tag="OO135_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            complement(16066..>16535)
                     /gene="ssuE"
                     /locus_tag="OO135_002392"
     CDS             complement(16066..>16535)
                     /gene="ssuE"
                     /locus_tag="OO135_002392"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5398211.1"
                     /translation="WNLQNFAPEDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAA
                     YSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVF
                     ADDSQVIDYHHRPQFTPNLQTRLDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4119 a         4294 c         4236 g         3886 t
ORIGIN      
        1 cataacggcg cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg
       61 tttatatact gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta
      121 ttggcgttgt acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac
      181 agggggcaag ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg
      241 tgcctggccg taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc
      301 cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc
      361 acggcaataa tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg
      421 caaatcgaat ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg
      481 ttgtatgccg cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc
      541 cagcaacagc tggcagaggt ggcgattgcc ggtttccagc cgcagtttaa taaatgggta
      601 gagttactga ccgatcctgg tgttaacggg atggcacgcg acgtagtgct ctctgatgcg
      661 atgatgggct atctccattt cattgcaaat attccggtca aaggcactcg ctggctatat
      721 agcagtaaac cttatgcact tgcaacgccg ccgctctcgg tgattaacca atggcagctg
      781 gcgctggata aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa
      841 tatgcggcga tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa
      901 ctgaccggca aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc
      961 gaaatattgc aacgcacagg catgttggac ggggggccga aaattactct acctggcgat
     1021 gacacgccaa ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact
     1081 aagccgatgg acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgcgccgcc
     1141 tacgataatg aactggtgga agccgttaaa cgttttcagg catggcaagg attgggggca
     1201 gatggtgcta ttggcccggc aacacgtgac tggttaaacg taacgcccgc ccagcgtgct
     1261 ggcgtgttgg ctctcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg
     1321 atcatggtta acattccggc ctattcgctg gtctactatc agaacggcaa tcaggtgctg
     1381 gattcgcgag tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgccctt
     1441 aacaacgtag tggtaaaccc gccgtggaac gtacctccaa ctctggcacg caaagatatt
     1501 ctgccaaaag tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgt
     1561 ggctggaaca gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc
     1621 tcgaatttac cgttccgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat
     1681 aaattcaata tgccgagttc agaggccatt tatttgcatg acacgccgac ccacaatctg
     1741 ttcaagcgtg atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat
     1801 ctggcgaata tgctgttgca ggatgcaggc tggaatgaca aacgtatttc tgatgcgctg
     1861 aagcagggtg atacacgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac
     1921 ctgacggcct ttgttggtgc agatggtcgt acccagtatc gtacagatat ttacaattat
     1981 gatctgcctg cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa
     2041 atgaagtagt tcggggaaat cagttgtcgt aataattagc atgattgggg gcgattctct
     2101 gcagcccccg tcactgctgg ggttgagtca tcttgacgtc tgctttacgg gcggttaagg
     2161 tgcctcttgt gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc
     2221 atggacaaat tcgacgctaa tcgccgcaaa ttgctggcgc ttggtggcgt tgcactcggt
     2281 gccgccatcc tgccgacccc tgcgtttgca acactctcta ccccacgccc gcgcattttg
     2341 acactcaata atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc
     2401 tatattcagg aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa
     2461 ataaagtcca tcgacccagg attattcgac cagttgtatc gcctgcaagg gttgttaggc
     2521 acgcgcaaac cggtgcaact catttccggt tatcgttcca ttgataccaa caatgaacta
     2581 cgcgcccgca gccgtggagt agcgaagaaa agctatcaca ctaaaggcca ggcgatggat
     2641 ttccatattg aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca
     2701 ggtggtgtag gatattaccc acgtagtaac tttgtgcata ttgataccgg gccagcacgg
     2761 cactggtagc aatcgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca
     2821 ccgcattctc tcagaactgt tcattaatct ggtgtgaaca aacccgtctg gccgcactgg
     2881 tcgatcctgg cggcgatgcg gaaaaaatca aacaggaagt tgatgacagc ggcctgacac
     2941 tgatgcagat cctgctgacg catggtcatc tggaccacgt tggcgcagcg gcggaactgg
     3001 cgcaacatta cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag
     3061 gcttgcctgc gcaaagtcgt atgtttggtc tggaagagtg ccagccgctg acgccagatc
     3121 gttggctgaa cgaaggcgat accatcagca taggaaatgt gactttacag gtgttacatt
     3181 gccctgggca tacgccgggt catgtcgtgt tttttgatga tcgggcaaag ctgctgattt
     3241 ctggcgatgt tattttcaaa ggcggagtag ggcgcagtga cttcccgcgt ggcgatcata
     3301 atcaactgat ttcttcaatc aaagataaat tgctgccact gggggatgac gtgacattta
     3361 ttccgggtca cggaccatta tccacacttg gttatgaacg cctgcataat cccttcctgc
     3421 aagacgaaat gcccgtctgg taaggcacat aaaaaagccc gcttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 atgcttacag cactgccaca atcgcttcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaaccg catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcagg ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcgcct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaac gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacggtttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcattcag gctgttaatc agtgcatcga
     4381 agtcaagagt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacact cgcttaacgc
     4501 tggtattttt tgccaggaaa tcggcagcta cgcgtagtgc gccagtgccc cccggagtct
     4561 gtgccgtgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gtataaaaaa aacaggacca aagtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgttg
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtatttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccttggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt ttttatgaca cctgccactg ccgtcaataa
     6181 gttctgtcaa taaaaattta cggaactatt gatgagagtt tggtgtcttt atgtgtctgc
     6241 aggcatcttt ccattcaaac taacgtttcg ctaccgtgaa agtgctgcaa agataaagat
     6301 ttagtttcaa aaagaaaaaa tatgtaacca aaagtaaaaa ttaaggaact ttgtgaacac
     6361 cgtcatattt ccatagaaac gtgatgatat ttacagcaat ttttatctat ttatatgatt
     6421 tacttatatt taaattaact aagcggaaat tttgtttctg atggaaactt tatcgacctg
     6481 gcacaaaatc ctctttcaga catccagaat gccaaaaaat agtatgaaaa tagtgctatc
     6541 agctaagaaa aaactaatcc gcaataagaa tgcgggttat ttttttgtgc gtaaatgttg
     6601 caatgtaata ttccgtaaca ggatgatcgt tatggcgggg ttgcaaatag attgcttgac
     6661 ttaacaaaca gataacttga cagaaaggat aaaaaagagc cagcaggcgc tggctctttg
     6721 gggaagaaaa tcagaagctg gcgttgcgcg gagtacgtgg gaacggaatc acatcacgca
     6781 cgttttgcac gccagttacg taagcaatca ggcgttcaaa accaagaccg aaacctgaat
     6841 gcggaacagt accgtagcga cgcagatcgc gataccacca gtaatcttct ttattcaggc
     6901 ccatttccag catacgctcg tccagaacgt ccagacgttc ttcacgctgg gagccaccaa
     6961 tgatctcacc gatgcccgga gccagaacgt ccatagccgc aacggtttta ccgtcttcgt
     7021 tgaggcgcat atagaacgct ttaatatctt tcggatagtt tttaaccact accggtgctt
     7081 taaagtgttc ttccgccaga taacgctcat gctcagaaga gagatcgacg ccccagtaaa
     7141 ccgggttttc aaacttcctg ccgcagtttt cgagaatggt cactgcgtcg gtgtaatcca
     7201 cctgcgcgaa atcggcctca atgaagcgtt ccagacgtga aacggcatct ttatctacgc
     7261 gttcagcgaa gaatttcatg tcgtctgcgc gttcttcgag aaccgctttg aagacatatt
     7321 tcagcatggc ttcagccaga cccgcgatat cgttcaggtt agcaaacgcc acttccggct
     7381 ccagcatcca gaattccgcc aggtgacggc tggtgttgga gttttcagca cggaaagtcg
     7441 ggccgaaggt ataaattttg gataatgcgc aagcgtaggt ttcgccgttc aactggccag
     7501 atacggtcag gaaagactct ttaccaaaga agtctttgtc gaaatccact ttgccctgat
     7561 cgttacgcgg caggttttcc agatccagcg tagaaacgcg gaacatttcg cctgcacctt
     7621 cggtatcaga tgcggtaatc agtggcgttg aaacccagaa gaatccctgc tcgttaaaga
     7681 agcgatgcag cgcctgcgcc agcgtatggc gaacgcgcgc gacggcacca atcaggtttg
     7741 tgcgcggacg caggtgagcg acttcacgca gatactcaat gctgtggcgt tttgccgcca
     7801 tcgggtaagt gtctggatct tcaacccaac cagcaacttc aactttgctg gcctgaattt
     7861 caaattgttg cccctggccc ggcgacgcca cgactttacc cgtcacaatg accgagcagc
     7921 cggtggtcag acgcaggacg tcttcattgt aattgggcag agaattattg atgacagcct
     7981 gtacaggatc aaagcaggaa ccgtcataaa cggcgaggaa ggagatgcca gcttttgaat
     8041 ctcggcgggt acgtacccat ccgcgtacgg tgacttcgct gtcaacggct acgcggccct
     8101 ggagtacgtc ggctacaggc acaacgctca taatattctc tctgttaata gtcggaaaaa
     8161 ataaacactt gtccacccgt aaatgggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agctttttat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact ggcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 attcttccag ccaggcagca agtgcagctc gctggctgtt ggctaggtcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgcgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggtg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aattcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcatcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccggatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctccg cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggttt gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccactg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt cacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgtgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccgatcc
    10861 gcccggacat ggtcattgag atgaacaact tctataccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgccat gatggcagcg cggcaacctg cgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtc gatctctccc atttccgccg ttggtacagc cagtccggca
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac cgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatctc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgct
    11461 gggatgcggc acaaagcctg ctggcaacct acatcaagct gaacgttgcc cgacatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgca gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcgaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcagttcc
    11881 acgaagcgaa caatatgacc gatgcgctgg cggcgctttc tgcggcggtt gccgcacagc
    11941 tgccatgccg tgatgcgctg atgcaggaat acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgcggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggcgc gtttgcgggc agcaatccgg cagcgttcca tgccgaagat gggagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtggcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga taaataaccg aatggcggca atagcgccgc cattcgggga
    12421 atttacccct gttttctcag gcgaatttca gattcaccac gctgcatcac cctttgcaac
    12481 acttccgatt ccagctccgc taatcgcacc gaccctaatc gacgcggacg ggggatatcc
    12541 actgtcaaat ccagaccaat ttttccctct tcaattaata acacccggtc agccattgct
    12601 accgcttcgc tcacatcatg cgtcaccaac agtacggtaa agccgtgctc ctgccaaagt
    12661 gacacaatca aatcctgcat ctcgagtcgc gttaaggcgt ccagtgcccc gagcggttca
    12721 tcaagcaaca ataaacccgg tcgatgaatc aacgctcttg ccagcgccac tcgctgtttc
    12781 tgcccgcctg aaagtgcggc aggccattcc ccggcgcgat tctccagtcc taccgcagcc
    12841 aacgcccgac gcgcggcatc gcgccactgg cctttaaggc ctaacccaac gttatcaatc
    12901 accgatttcc acggtagcag acgcgcatcc tgaaacatca ttcgcgtgtc ttcctgaatt
    12961 tcagccagcg gtgtggtgcc tgccaacaca tcgcctgcgg ttggcgtttc cagcccggcc
    13021 agcaggcgca gtagggtact tttgccacca ccgccacaaa ctgacctgcc ggaatatgta
    13081 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13141 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13201 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13261 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actctcgcgc
    13321 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13381 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13441 caggatcaca tggataaaca gcggtatgcc ggataaccca tagctacgcg ccatctccac
    13501 cagcccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg gaaacagcgt
    13561 accgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13621 cgggatcagc gccagatgcg gcacgttgcg caacatctga attgaggtat ccagcagccg
    13681 ttctccccag cgagatagcc cgctaatcag tcccaaaatc aaccccagcg atccgccaat
    13741 tgaaaagcca atcagcgccc gctaggaact gatcgccaga tgctgccaca gttcgccgct
    13801 ggcggagagc gtccagaacg ccgtcaccac cccttccggt gagggcaaaa tacgcgtcga
    13861 cagccagcca actgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    13921 gggggcaacg cgcaataacc acttcttcac tggcgttgcc attatttctt ccttagcttt
    13981 gcgcgacttt acgggggata aaatcattcg ccaccgcttc gccttgcgga ttcagcggct
    14041 gcggctgggg aatttccgga atggcgacat ccagatgtgg gaacagcaac tcgccgaccc
    14101 gatacgcttc ttccagatgc ggatagcccg aaagcacaaa gctgtcgatg ccaagcgcgg
    14161 cgtattcgtt gattcgcgca gcgaccgtag gaccatcgcc caccagcgcc gttccggcac
    14221 cgccgcgcac taagccaacg cccgcccata aattggggct gatctccaga ttgtcgcgct
    14281 tgccgttatg taacgccgcc attcgctgtt gtcctacgga atccgtccgg gcgaatgcgg
    14341 cctgtgcttt ggcgatagtt tcatcatcaa gatgcgagat taaccgctcg gcggcctgcc
    14401 acgcttcgtc gttagtttca cgaacaatca catgcagacg aataccgaaa cgaattttgc
    14461 gtccatgcgc ggcagctttc gcccgcactt gttcgatctt ctcttttact agttctggcg
    14521 gttcgcccca ggtgaggtag agatcaactt gttctgccgc cagctcctgg gcgacatctg
    14581 acgatccgcc aaagtaaagc ggaggataag gctgttgaat cggcgggaag agcagttttg
    14641 ctccgcgcac atgaatatgt ttaccgttga aattgacggt ttcccccagc aataagcgcc
    14701 gccagaccta ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14761 cgtcgcctgc cagctcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    14821 ttgagagacg gtcaagcgtg gcggcctggc gagcggcaac ggtaggtgag gttacgctgg
    14881 ggcgcagggc gacaagaaac ttcagccgcg gcgtcaccgg gatcatcgac gccgccacca
    14941 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15001 ccgccgcttg cgcaatttgt tgcagataac cgtgatcaac cgggcgtgaa ccttcttccg
    15061 ttccaagata gtgcccgtca ccgtgggtcg gtaaaaacca gaacatattc agactcataa
    15121 ttgttttcct tccagttgag tgggctgcca gatgcgctgg cgaatatcga cttttttcgg
    15181 caccagacga ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacctcggc
    15241 gttaaccggt ttgatggtgg taggagggcg atgatctaag taagaggcaa tcaccggtgc
    15301 cggtaagccc atcgtttttg ccagtaaagc gatgctttgc tcgcgctggc tgcgggttaa
    15361 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt tttttctgca
    15421 tagggacgag ctgccagata aaacgatcca gtttgattga gatcggtgcc gtctttcagc
    15481 acccgcacgc cgccctgtaa taatgcagca gagtagtagg gatcccagat agcccaggcg
    15541 tcaacgttac cttgctggaa cgcggcgcgg gcatcagcgg gcgtcaggta agtgggttgg
    15601 atatcggtaa acttaagtcc ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg
    15661 gaacctttct gaaaggcaac tttgtgacct ttaagatcgg ctacggtttt gatcgggctg
    15721 ttttctgcca ccagaatcac ttcggctttg ggcttcggtg gctcgacgcc cacatatacc
    15781 aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact gccgagatca
    15841 atactgccaa cgtttaaggc ttccaacatt tgcggacccg cggggaactc cacccaggag
    15901 atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccag taccatacca
    15961 atactgcctt tctgatagcc tatacataac gcttcaggcg aggattctgc agcaaccgca
    16021 atcgtagaga cgctaagcaa tcccaccagc gccagtttaa tgatgttacg catgggcatt
    16081 acctcgcaga gacagaaggt caggaacctg aacatcgcgg cggtgcaatg cctgccagaa
    16141 agtttctagc gcggtatcaa gacgggtttg cagatttggc gtgaactggg gtctgtgatg
    16201 gtaatcaatt acttgtgagt catcggcaaa cacgccgtgc aggatctcct gagctttcag
    16261 tgcgcttaaa actggtttaa gggcataatc gaccgccagc agatgggcca cggtaccgcc
    16321 cgtcgccagc ggtagcacca ctttgccttg caaagcgcgc tctggcagca ggtcgagcag
    16381 ggttttcagc gcgccggaat aggcggcttt atacacaggt gtagcgacta tcagtccatc
    16441 agcctgttgc agctgttcgg tgaaggtctt gagtgccgga ctatcgaaac gagcataaag
    16501 cagatcttcc ggggcgaagt tttgcagatt ccagt
//
LOCUS       DAJTMK010000087        16501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTMK010000087 DAJTMK010000000
VERSION     DAJTMK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.088"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..749)
                     /gene="mak"
                     /locus_tag="OO135_002393"
     CDS             complement(<1..749)
                     /gene="mak"
                     /locus_tag="OO135_002393"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5398212.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVL"
     gene            874..1785
                     /gene="rdgC"
                     /locus_tag="OO135_002394"
     CDS             874..1785
                     /gene="rdgC"
                     /locus_tag="OO135_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5398213.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(1863..1946)
                     /locus_tag="OO135_002395"
     CDS             complement(1863..1946)
                     /locus_tag="OO135_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398214.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(1943..2284)
                     /locus_tag="OO135_002396"
                     /pseudo
     CDS             complement(1943..2284)
                     /locus_tag="OO135_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2432..2716)
                     /gene="ppnP"
                     /locus_tag="OO135_002397"
     CDS             complement(2432..2716)
                     /gene="ppnP"
                     /locus_tag="OO135_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5398215.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2788..3465)
                     /locus_tag="OO135_002398"
     CDS             complement(2788..3465)
                     /locus_tag="OO135_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5398216.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3723..3914)
                     /gene="yaiA"
                     /locus_tag="OO135_002399"
     CDS             complement(3723..3914)
                     /gene="yaiA"
                     /locus_tag="OO135_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5398217.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3964..4488)
                     /gene="aroL"
                     /locus_tag="OO135_002400"
     CDS             complement(3964..4488)
                     /gene="aroL"
                     /locus_tag="OO135_002400"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5398218.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTING"
     gene            complement(4671..5129)
                     /locus_tag="OO135_002401"
     CDS             complement(4671..5129)
                     /locus_tag="OO135_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5398219.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5249..6058
                     /gene="proC"
                     /locus_tag="OO135_002402"
     CDS             5249..6058
                     /gene="proC"
                     /locus_tag="OO135_002402"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5398220.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6075..7190)
                     /gene="adrA"
                     /locus_tag="OO135_002403"
                     /gene_synonym="dgcC"
     CDS             complement(6075..7190)
                     /gene="adrA"
                     /locus_tag="OO135_002403"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5398221.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7292..7612)
                     /gene="psiF"
                     /locus_tag="OO135_002404"
     CDS             complement(7292..7612)
                     /gene="psiF"
                     /locus_tag="OO135_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5398222.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSGCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7731..9146)
                     /gene="phoA"
                     /locus_tag="OO135_002405"
     CDS             complement(7731..9146)
                     /gene="phoA"
                     /locus_tag="OO135_002405"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5398223.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQSVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9247..9507)
                     /gene="iraP"
                     /locus_tag="OO135_002406"
     CDS             complement(9247..9507)
                     /gene="iraP"
                     /locus_tag="OO135_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5398224.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(9734..9891)
                     /locus_tag="OO135_002407"
                     /pseudo
     CDS             complement(9734..9891)
                     /locus_tag="OO135_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9969..11063
                     /gene="ddlA"
                     /locus_tag="OO135_002408"
     CDS             9969..11063
                     /gene="ddlA"
                     /locus_tag="OO135_002408"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5398225.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11087..11299)
                     /locus_tag="OO135_002409"
     CDS             complement(11087..11299)
                     /locus_tag="OO135_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5398226.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            11559..11867
                     /locus_tag="OO135_002410"
     CDS             11559..11867
                     /locus_tag="OO135_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5398227.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(11926..13020)
                     /gene="yaiW"
                     /locus_tag="OO135_002411"
     CDS             complement(11926..13020)
                     /gene="yaiW"
                     /locus_tag="OO135_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5398228.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPDLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(13033..14253)
                     /gene="sbmA"
                     /locus_tag="OO135_002412"
     CDS             complement(13033..14253)
                     /gene="sbmA"
                     /locus_tag="OO135_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5398229.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            14605..15762
                     /gene="ampH"
                     /locus_tag="OO135_002413"
     CDS             14605..15762
                     /gene="ampH"
                     /locus_tag="OO135_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5398230.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(15763..16386)
                     /gene="iprA"
                     /locus_tag="OO135_002414"
     CDS             complement(15763..16386)
                     /gene="iprA"
                     /locus_tag="OO135_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR5398231.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
BASE COUNT         4120 a         4288 c         3946 g         4147 t
ORIGIN      
        1 aggacaatca catccggatc gagaatattc acgacatgtg ccagcgattt tgccagccgc
       61 agctcgtagc gacgcaatgc cagttccgct accggatcgc tttcttcaac caggcggata
      121 atttcactgc ctttcagcgc atgtccgctc aaacgacgat aatccatcgc gaatcccgtg
      181 cccgaaataa aggtttcaat acaaccttgt ttaccgcaat aacaagggac ttcctcgcga
      241 taacgcagtt cgtcttcgtc catccacggt agcggattgt gtccccactc acctgccgtg
      301 ccattgccgc tgatatgcgc ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg
      361 ataatcacgg caaataccgt ctgcgctccc gctgccgcgc catctactgc ttctgaaacc
      421 gccagacagt tagcgtcatt tgccagccgc acttcccgct gcaacctcgc gcttaagtct
      481 ttatcgaatg gctgaccgtt gagccaggtt gaattggcat tcttcaccac accggtgtaa
      541 ggcgaaattg agccaggaat gcccatacct accgttccgc gctgccccgt cgcctgctcc
      601 gccatatcaa ccaacgtggc gatcgtttca atagtctgcc ggtaatcatc acgcggcgtg
      661 ggcagacgat ggcggtacaa ctgctcccct gcatcgccca gtgcaatcac ttcagttttg
      721 gtgccgccta aatcgatacc tatacgcacg gtactctcct tatttttttc aatatcaata
      781 gcgtagagac ggacaaccgg attggcaatg caaggccgcc gacaattcgt tatcatgccc
      841 gctaaattta acaacaaggc cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt
      901 taccgtctta gccgcgagat ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg
      961 atggcattta ccccatgcgg cagccaggac atggcgaaga tgggctgggt tcctccgatg
     1021 ggatcgcaca gcgatgcgtt aacgcacgtt gccaatggtc aaattgttat ctgcgcgcgc
     1081 aaagaagaaa aaatcctccc gtctccggtg attaagcagg cgctggaagc gaaaatcgcc
     1141 aaactggaag cggaacaggc gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac
     1201 gaagtgctgc actctctgtt gccgcgtgct ttcagccgtt ttagccagac aatgatgtgg
     1261 atcgacacgg ttaacggttt gattatggtg gactgcgcca gtgccaaaaa agcggaagat
     1321 acgctggcac tgctgcgtaa aagcctgggg tcgttacctg tggtaccatt gagcatggaa
     1381 aacccgattg aactgacgct gaccgaatgg gttcgctccg gtagcgcggc acagggcttc
     1441 cagctgcttg atgaagccga gctgaaatcg ttgctggaag atggcggcgt gatccgcgcg
     1501 aagaaacaag atctgaccag cgaagagatc accaatcaca tcgaagccgg aaaagtggtg
     1561 actaaactgg cgctcgactg gcagcagcgc attcagtttg tgatgtgcga cgatggttcg
     1621 ctcaagcgtc tgaagttttg cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa
     1681 gatttcgccc agcgttttga tgccgatttc atcctgatga ctggtgaact ggcagcgtta
     1741 attcaaaacc tgattgaagg attaggtggc gaagcacaac gttaattgct gattttcctt
     1801 taatgccgga tgcgacgcct gccgcgtctt atccggcgta cgaagccaca ccagggatat
     1861 aattattcgc tacggcggac aataattttt agcgcagcaa tattatgcgt tttatgctgt
     1921 aacttgctcc atggacgttg tgtcattgtt tttcctcaag ccagtgacgc gcttcactgc
     1981 gaattttccg cattgccccg gttttcacta aatcccgtaa cagcgaaaga tcgggttgtt
     2041 cgacaaataa gagtaatgat ggagcctctt ttaaccacgt cgttttcaca tcggcagcga
     2101 gctgtggatt attcatagct agcgaccgat gccgagactc ggtcaatgtc gttaacaacg
     2161 acgccggggt atgtgcgttg cgtaatgccg tttggcgcaa atgatcattg cgactttgca
     2221 gcaatgcacg ccaccattca tcatctgatt tatcttttat cgtagccagt tccgcaacaa
     2281 tatctggaga tcttcactga tatttatatc aaccttgatg aattatgaat atatcggaaa
     2341 ttgaaaaaat cgcagcaaag gaaatattat tatgggttaa agagaggata tttcagaaaa
     2401 atgccctccc gttcaagggg aaggcgagga attacagata gcggcacaga taagaggtgg
     2461 gttcggcaac ttgcagatga aactcactgt gaccgggaac attaaacacc gaaccggctt
     2521 catacacctg ccagtcggtc gcatcaggca gtaacacatt cagcgcgcca ctgattaccg
     2581 tcatctcttc cggctcagcg gtgctgaagg tgtattcgcc ttcaaccata acacccacgc
     2641 tggcgcgacc agtgctgctg ctggaaaagc cgattgattt cactttgccg gaaaagtact
     2701 cattactttg aagcataaac tggccctgtc tggtagttga acatctactt cactatgggg
     2761 gccagaggcg ctggctgtca cgcaaaatta caccagtaat tccgcagcca gccgtgcaat
     2821 caatacgtta gacagcaaga caggaacatc gagctgtttt tgcagtaaat cgcgatgacg
     2881 ttggtgaaat cctaaacaat ccagcatgat gacatcagcc ccttttgcca gtaattcttt
     2941 cccggcatca atgatttttt gttctgaatc atgaatgggg ttacccaatg aaaataccgg
     3001 cgatttctgc aaaatttgcc atttttgcgc ctgaacgggc agcatctcct caaccggaac
     3061 gataaccccc acctgatgat cttcaacaat agaggaaacc agtggaggca atattcgcga
     3121 cggctcaaga aagatcgtat tacgcgcagt catactacta atgtttgctg tactcattaa
     3181 taaaatgacg tcataaccct gattatcgag cacttcaacc acaccttgca ggtctcgctc
     3241 cactttacga cgcgaaacat gggccagctg gttgtcattt aataatgtga gaatggtgtc
     3301 ttcacctgct tctggcgcgt actccgccat cacttcttca cgacttaact tccccagcag
     3361 gctatgatgg gaaatattat cttcgtcaat gtattccgtc aggagcggca aaacctcctg
     3421 cataggtaca atgccgatgg tcaggatcgc caacgacgca ctcattgttc atccacctta
     3481 cttttctttc gtcgttaatt accgggcaag tgtgaagcac cactgctgac acttacttgt
     3541 gctacaaatg acaaaaagcg tagcagcaga cgtgcacggc ataatgtaaa gattccaaat
     3601 gattccagta atggattgtt attgtttaat attctaatta ttagaaaaac atgaattatg
     3661 aaaaatgtga cgcagatccc ttgtagtggc actcagaatc ccttccgagc agtctgatgc
     3721 ggtcactctt tgtcagggtc ctcatagcgt tttttcgcat ccatcgcttc ctgagcggtc
     3781 ggatgttcac tgatcaacga gtctggttta ggatgatcgg ctctgagttg gtaccaggtt
     3841 accgtctgtc ctggctttcc tttttcaatc gtcactatat atgcttcacg aggataaggc
     3901 ggtttcgttg gcatcgttct tccttatttc acggaatgaa cgttaagtat aggcgctcta
     3961 aaatcaacca ttgatcgtct gtgccagggc gctacgaatt tcagaaatca cctggctggg
     4021 ttcgtttgtt gcgtcgatga taatatgcgc aacttcgcga tatagcgcat cgcgttcttc
     4081 cagcacttcc tgaacttctt cgctcagcgg ttttcccgtt aaggttggcc gtaaatcttc
     4141 ttccggtgca gcttgcagtc ggttaaccag gactgatact ggcgcacaca aataaaccac
     4201 gatcccgtta ttttgcatga agtgacgatt aaattccgtc agaataatgc cgccgcctgt
     4261 agcgataacg gtggatggcg cagttaccgc ttccagcgcc gccgtttctc tggcgcgaaa
     4321 tcccgcccac tcttcccttt cgacgatctc cgcgaccgtc atattgagct gtgattgcaa
     4381 ccactgatcg gtatcgacaa accgacggtt aagcgaatcg gcaagggcca ttccgaccgt
     4441 tgttttacca cagccccgag gcccgatcag aaaaagaggt tgtgtcatcg tgggttttcc
     4501 ccaataggtc gcaatgcggc gaaagccggt gtcatgagaa tagcgatcat tccatcaaac
     4561 tagtacaatt tcgattgtaa agaaaaaatt ccacttaaag tgaaaatctc aatacatccc
     4621 ttactatacc aataaatatt caagaatgaa gtgtaaataa taaattacat ttagccacga
     4681 ctacgttgca cttccagcca ccacttctcc agctccgcgg caaaggcctg gcggtcacgt
     4741 tgtgaaaggc tatctggtcc gccggtctgg atcccactgg cacgtaaggt atccataaaa
     4801 tcgcgcatcg tcaggcgctc acgaatggtc gctggcgtgt aacgttctcc gcgcggatta
     4861 agcgccgctg cgcctttctc gatggcttca gcagccaaag gtatatctgc ggtgatcacc
     4921 aaatcgcccg cttcacactg ccggacaatt tcgttatcgg caacgtcgaa acctgccgcg
     4981 acgcgcagcg tacgaataaa tcgcgatggc ggcacgcgta aactctggtt tgctaccagt
     5041 accagcggca tctgcatacg ttccgccgcg cgatacaaaa tctctttaat tacattggga
     5101 cacgcgtcgg catccaccca aattgtcata aagtcatcct ttgttgggta atcctctatt
     5161 gtgtcgcgct tttgccttcc ggcatagttc tgtttatgct tctgccagcg attatcaaaa
     5221 caatgaattt cacggcagga gtgaggcaat ggaaaagaaa atcggtttta ttggctgcgg
     5281 caatatggga aaagccattc tcggcggtct gattgccagc ggtcaggtgc ttccagggca
     5341 aatctgggta tacaccccct ccccgaataa agtcgccgcc ctgcatgacc agttcggcat
     5401 caacgccgca gaatcggcgc aagaagtggc gcaaatcgcc gacatcattt ttgctgccgt
     5461 taaaccaggc atcatgatta aagtgcttag cgaaatcacc tccagcctga ataaagactc
     5521 tctggtcgtt tctattgctg caggtgtcac gctcgaccag cttgcccgcg cgctgggcca
     5581 tgaccggaaa attatccgcg ccatgccgaa cactcccgcg ctggttaatg ccgggatgac
     5641 ctccgtaacg ccaaacgcgc tggtaacccc agaagatacc gctgatgtgc tgaatatttt
     5701 ccgctgcttt ggcgaagcgg aagtaattgc tgagccgatg atccacccgg tagtcggtgt
     5761 gagcggttct tcgccagcct acgtatttat gtttatcgaa gcgatggccg acgccgccgt
     5821 gctgggcggg atgccacgcg cccaggcgta taaatttgcc gctcaggcgg taatgggttc
     5881 cgcaaaaatg gtgctggaaa cgggagaaca tccgggggca ctgaaagata tggtctgctc
     5941 accgggaggc accaccattg aagcggtacg cgtactggaa gagaaaggct tccgtgccgc
     6001 agtgatcgaa gcgatgacga agtgtatgga aaaatcagaa aaactcagca aatcctgatg
     6061 actttcgccg gacgtcaggc cgccacttcg gtgcggttac gtccggcttt ctttgctttg
     6121 taaagcgcca aatctgccga tttcaaccac tcacgatagt gactcatttg tgggttcagc
     6181 ggcgcaaccc ccacactaat ccgtaaagtt acctgtggcg tattcggcaa gcgtaacgtg
     6241 tttagccctt cacgcacccg taacatggcg gtaatggcgc tctcagctgg cgtaccggac
     6301 atgattactg caaactcatc gccgccaaac cgaccaatca catcgctacc gcgcagggta
     6361 atttgtaact gtcgggtaag cgccacaatc gcttcatcgc ccacatcatg accccaggta
     6421 tcgttgatgc tcttgaaatg gtcgatatcg ataatcagta acgttgcatc gcgattatgc
     6481 cgccgacagt tatcaaattc attgcgtaac atagtttccc aatgacgtcg gttatacacg
     6541 cccgtcatgc cgtcgcgggt actcatgacc tgcaacctgc gtttatgttc cgccagtttg
     6601 gttgccgtct ggtagctgac ccagccaaac agcagaggat aaacgacaat aatgggaagg
     6661 gagagccacc attccagcgg cgcactattg aacgacacgg taatgcccgt aagctcgagg
     6721 gtgacaaggc aggaaaccac catcaacacc agacccgcga caaacagacg ggggccgcct
     6781 gcccccatca aattcagaca cataatcatc aacatagcgg tggaaggcag cacgtttacg
     6841 cccattacgc ctacccacat tcccgctaat actgcatcgg tttttaagtt gtaaatttcc
     6901 cggctaagcg gatcgacggc cctgctcgct atctgccagg ctaaatgcgg ccagacgaac
     6961 gcccagccga ccaacaccag ccaccaccag cccggcggcg ggtgcgaaac cagcgttgaa
     7021 gcaatcggta agaacatgcc agccaggcca accgcacggg gtagtctgac gcgacgggcg
     7081 aagcgcagcc cggaccgctg atgttcgttt tgaggagtta aaggaggttc ctccccatgc
     7141 gccgccgctt ttttgaaaaa gttttcatca ttcattattt ttgggaacat tcagaaacaa
     7201 ttttcccaaa ttatagagac ggggctaaag gcagaagtat gaaatttcgg gtgagccact
     7261 ttcgtagctc acccgttagc actatcaggt atcaggcggc tttcttgagg caggcactca
     7321 taaacttatt acgatcatca cctttcagag attgttgcgt tgcttgatta ttacattcgc
     7381 gcatcttttg ctgctgtggc gtcaaacttt tttcaccagg cgcagacttg ctgttcttca
     7441 ggcaaccact catgtaggtc ttacgagcat cccctttcaa cgcctgcgcc gtcgcctgct
     7501 gattacagga ggtcatacgc tgttgttgtg gggttaacgt tctctcggca gcgccgactg
     7561 tggttaaaaa aaccagaccg aaaagcaagg taaccagtaa tgtaattttc atagcaccat
     7621 ccctcttcat gttttaacca tgagcgtatg cgcccgagat ttgccattaa gtctggttgc
     7681 taacagcaaa aaaaccaccc ggcagcgaaa attcactgcc gggcgcggtt ttatttcagc
     7741 cccagggcgg ctttcatggt gtagaagaga tcggtctggt cggtcagtcc aacgacattg
     7801 gcggcatgcg ggccatacgc cgcaatacgc aactgactgc cggtatgttc ttgtgaatcc
     7861 tcttcggagt tcccgtagct catcaccatc actgcgccat ctttggtatt tagcgcctgg
     7921 gtgaggcccg gagctttggt atccggcgcg acactctggc tggcgtgggc gtgatcagcg
     7981 gtgactatga ccagcgtgtt gccatccttt ttagcgaatt ccagcgcccg ttgtacggct
     8041 tcatcgagat cgaccgtctc gccaatttgc ccacaaggat tcgcagcgtg atcctgttta
     8101 tcgatcgacg caccttcaac ttgcaggaaa aatcctttct catttttact caacaattca
     8161 atggctttgt cggtcatctg cgccagggtt ggtacactgt cattacgttg cggattaggc
     8221 gtacaggtga ctgcgggctt atcgatattg ccgtggtacg ttgctttcgg tcctagccag
     8281 cgcactggca tattgccgtc agcaaacagt ccaagcagag gtttttgctg attcgcttcc
     8341 gtcaccgcat ttagcgaggc ggcatcgctg accaactgat aaccacgcgc ctgtgcctgt
     8401 tcacgcagcg tttttccctg ccattcaccg gcggttgccg tttcagcaaa ggtttttgcg
     8461 ccaccgccaa gcgtaacgtc ggcacgagca ttaagcagct gttcggtaat cgatcctttt
     8521 ccgccttttt ccagagcgtt acccggacat ttttcactgg tcgcgctcgg accgtagcat
     8581 ttgcgcgaag tcacatgtgc caccagcgca gcgggcgtgg catcctgcaa ctctgcggta
     8641 gaaacgttac cggtcgccag acctgcggct tttgccattt ccagaatcgt tgggtgatct
     8701 ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt tgacaccggt tgaccaggcg
     8761 gttgctgatg cagccgaatc ggtgacgtag tccggtttgc cggttttttt attcagcgca
     8821 tagtgagtgt attgcccggt aagcggtaag gcatcgatac ctttaaaaaa gccgcccgca
     8881 ccttcggcat aattacgtgc ggctgtaatt tccgaatccc ccatcccatc gccaatcagc
     8941 aaaataatat tttttgcagg tttatcgcta agagaatcac gcagagcggc agtctgatca
     9001 cccgttaaac ggcgagcacc gccgggtgca gtaatatcgc cctgagcagc ccggttttcc
     9061 agaacaggca tttctggtgt ccgggctttt gtcacagggg taaacagtaa cggtaagagt
     9121 gccagtgcaa tagtgctttg tttcacttta ttttctccat gtacaaatac attaaaaaat
     9181 aaaaacaaag cgactataag tctcggccgt gacaacttta tgacagctgt tgaaaagatt
     9241 aactttttac tgacgaggat gcttcaataa cttctttaca taattgcgca gcagctccgt
     9301 atcgtcgtca ggaatgctgg catcaggctt tacctcgtaa agagccccct ccacctgttc
     9361 aatcaagcgc tgttggtcat tttgtgccat attgcgaagc attgcagtga cgataatctc
     9421 caaagcttct acctgcgcgc acagttcttt cgactcttct tctttttggg caagctttaa
     9481 taacaactca gcaatgagat ttttcatgtc tgtatttcct tatccaacgt atggagaagt
     9541 taacattatg tttcacaact tcatagtgtc tggaagtatt atttttatga caaaggaaat
     9601 actttgcgca aatgttcttt gtttgattac cagcgaaacg gttctcatat ttaaataatt
     9661 aaccacacaa agaacaggcc tttaattatc aacctttatt ataaacaaaa gttatgaaat
     9721 atattctctg cgtttactta caactaagag aaaaaactca ccgcatggcc atttaagcca
     9781 ggaataaatt agcttcccgt tcatcatttt tgtgatctcg cgcacatctt tgtgataacg
     9841 cactttcttc accattacgt tgcacgcttt gtcagcagaa gattgcaaca ttctgtcaga
     9901 aggccgcgaa tcccccgttt tgtcgtggta atgtatgcac tttgtgttag acgggatagg
     9961 tttttaagat ggaaaaactg cgggtaggaa tcgtttttgg tggtaaatca gcggaacatg
    10021 aagtgtctct gcaatcggca aaaaacattg tcgatgccat tgataaaagt cgcttcgacg
    10081 ttgtgctgct gggcattgat aaacaagggc aatggcacgt cagcgatgcc agcaattatc
    10141 tgctaaatgc agacgatcct gcccatattg cgttgcgccc ttcggcgact agccttgcgc
    10201 aggtgccaag taaacatgag catcagctta tcgacgcgca aaacggtcag ccgttaccga
    10261 cggtggatgt cattttcccg attgtccacg gtacgctggg cgaagatggt tccttgcagg
    10321 gaatgctgcg ggtcgccaat ttaccgtttg taggttctga tgttctggct tcagcagcct
    10381 gtatggataa agatgttacc aaacgtctgc tgcgcgatgc tgggctgaac attgcgccat
    10441 ttattaccct gacgcgcgcc aatcgtcaca acattagttt tgccgaagtg gagtctaaac
    10501 tggggttacc actgtttgta aaaccggcta atcagggctc ttctgttggt gtcagcaaag
    10561 taaccagtga agaacagtac gcaattgccg tcgatctggc gttcgaattc gatcataaag
    10621 tgatcgttga gcaagggatc aaaggtcgtg agattgaatg cgcagttctg ggcaacgaca
    10681 acccgcaagc cagcacctgt ggcgagatcg tactcaccag cgacttctat gcctacgaca
    10741 ccaagtacat tgacgaagat ggcgcgaaag tggtagttcc ggcagccatt gcgccagaaa
    10801 tcaacgataa gatccgggcg attgccgttc aggcttatca aacgttggga tgcgcaggca
    10861 tggcgcgtgt agacgtgttt ttaaccccag agaacgaagt ggtgatcaac gagatcaaca
    10921 cactgcctgg cttcaccaac atcagtatgt atccgaagct gtggcaagcc agcggtctgg
    10981 gttacaccga tctgatcaca cgtttgattg agctggcgct ggagcgtcac gctgcggata
    11041 acgcattgaa aaccacaatg taatatttta acgcccgata atattcttat tgggcgtttt
    11101 cagtttcttc atcgcgacga cgaataatca accccgccag ccagaaacta atcacccacg
    11161 tcaccagacc gacggcatag gtctgccaac cttttgcttc aaatcccagt aaccccacca
    11221 cgccattaag aatgaatata aggccaatgg cgaacgcata gtagtgccag tcacggcgga
    11281 tttttacagg cagattcatg gcagctccgt taacgaaaaa caacatcctt gcatattctg
    11341 caacaaccgt ctgtggtgta gacaacattt tcgtaattgt taataaattt aacttaaggt
    11401 tgccattttg tgactcacaa cgaatattca cgcttcggga atattcccaa catcaattta
    11461 ccatgattct gatattgaca atctaaatga cctgtcagac ttgccataag ccgctggtat
    11521 cacaccagaa gagaagcaga tgacttccgg aggtttttat ggctgatttc accctgtcaa
    11581 aatcgctgtt tagcggaaaa tatcgcaatg cctcttcaac gcctggcaac attgcctatg
    11641 cgttgtttgt gctgttttgc ttttgggctg gggcgcaatt gctgaacctg ttagtgcatg
    11701 cgcccggcgt ctatgagcgt ttaatgcagg tccaggaaac aggtcgccca cgggtggaaa
    11761 ttggtttagg tgtcggcacc attttcgggc tgatcccgtt tttagtaggc tgcctcattt
    11821 ttgcagtggt ggcgctatgg ctgcactggc gacatcgccg ccagtaagat ttacgttgcc
    11881 agatacggcc taaaacgcct tatctggcat gacattcctg tttgatcact gtttcataca
    11941 acgcgcccgc cgttcgtcta cgcgcttcgc aaaccaggcc gtagtcaggt tgcgggtgat
    12001 tttcgggctt tccagttgaa tgccaggcaa catttcgcgg ggtagtgatt tacccgtttt
    12061 cgcttcggca agttgataaa ctttcttgta cagcgccgtc tcctcaaaag agaagctgtc
    12121 acctttctct aactgacggc gaatttcgct gtcgctcatc cccagttttc ccgccagttt
    12181 gcgcgttgcc agttcggttt tcccgggttc tttactgtcg taacgaatta aatcaccgtc
    12241 cagtgccagc ttcacgccgc tggccttact gaccgcgttc tgaaacgcgg cattacggct
    12301 ggcgtaccag ccagcgttaa aatcagcaaa gcggtatatc ggtgcgctat agctggcggg
    12361 ataattcagt aaatggtaag taccaaacca caacccgccg cggcgactga agacttcctg
    12421 acgaactgta ccgtccattt tccacggata ccctttggta tgctgttcgg caaaagcaat
    12481 gctgacctgc atcggcccac cggtgcgcac cggattaagc gaaccaaaca gcgtctgccc
    12541 cattggtacc atgctgatca gatcgtcaaa aatcgcgctc aattgctttt ctgtacgcac
    12601 cgaatctaac cgttcgctat aacttttgcc gtttggcgat ttgattttta gcgcggtatg
    12661 aaccagaaaa gcaggaatgt gcatccgttc agcacgacgg tcaatctctt gccaggcgat
    12721 cttgcttaag tccggaacgg ccggatctgc ctgataattt gactcctgct gcgccaccgc
    12781 cagcaccgag cagacgtttt ccagcgtcgg tgccagcccc tgactttcaa aggtggtggc
    12841 gagatctttc gcccaggcat cgcggtcttt cacgctcgca ggcatcttct gccgcacgac
    12901 gctcgctaca tctatcgctt tttcgccctt tttcaacggt tgcggagcct gactactaca
    12961 ccccgctaaa accagaaccg ccaacaatga cagcgaagat agatgattga cgcgcgacat
    13021 tacttctcct ttttagctca aggtatgggt tacttcctga attttgtcac catccagctc
    13081 atgttcaaag ctgcgcagac gtttgtagat agacatcaac tcaaccagtg tggtccatga
    13141 gttaatcagg tactggaaag caccgcgaac ctgaccaaaa acgttggtaa tctgcgtcat
    13201 caggccgagc gtaatcgtac cggcaacaat tgacggaaac agcaagaaca aaccgaaaac
    13261 gttatcgacc tgcaaataga gaatgcgggc gatgttgaaa tacatatagt gaaaatagag
    13321 gcgaaaatag tttttccgta cggcgctaaa cagctcgcgt accgtaggcg gcgtcgcgcg
    13381 cgtggcatcg tcttcaccat aaaccagctc tttacggtag gcggcctcta ctcgctggtt
    13441 tttaaactcc agccccggca gtttgatccc taccactgcc agcaatccgg tccccatcag
    13501 cgaccagacg atagcggcaa tcaccagacc atacggaatg tgcccgataa tcggcagctc
    13561 cggcacatgc gcggagagcg ttaccagcac cggcaggaag gcgaccaacg tcatgatggc
    13621 gttgataaaa ctgacgccca tattctccag cgttgaagca aaacgcatgg tgtcttcctg
    13681 cacacgctgt gcggcacctt cgatatgacg cagttgttgc cagttcgcca tgtaatattc
    13741 gttcatcgcc gtacgccagc ggaacacata gtgactgaca aagaagttgt tcagtacact
    13801 gatcaccacc gcgatcagcg caatccccag aaagacgccc acttcgcggt aaaattgttc
    13861 gatagtgact ttatgcggcg aacttagcgc ggtttgaatc agatcataga acggtgcata
    13921 ccaggcgttg acggcgaccc cgacttccac caaaaaccag gtgacgaaga tgatcagtgc
    13981 agtaccgagt attgaccagt attgccaacg gtgcgggctg tagataaacc agaaaaatgc
    14041 aaaaagtcct acgcaaacaa tgtagtaagc gtaaaaaatc aggaaatcca acgaccagaa
    14101 acgcgcggcg ctaatcggga tctgcccgga agcgccggtg atacgcgcca cccagtctcc
    14161 cccaccggct tgccagaaga taacggcaat caatgcccaa acaaaggccg agagaaaaaa
    14221 cgctcccggc tttgggaaaa aagacttaaa catatgcact cctgctaact tcttatcgtt
    14281 ttaacaactg ctgtgtatta accgcatgac cactacaaag cgtcagccaa atgaaaccgc
    14341 ccattgtgac ccatagcaag gcgcttagtt cgccagcctg accataagaa ttgtttcgac
    14401 ggatttcaca aaccaaagat tgtttacata agcggattaa cacgcgcagg atcacaaatg
    14461 caggcattgg tgagcaggaa aaagctcgtg tttacgcgcc gttacgtttt taacactatt
    14521 attgcactaa ttcgcccttt gcaatccatc aatgagtagt ataaatacgc tcagttacct
    14581 tcattcaatc tatggacacc accgttgaaa cgtagtctgc ttttttctgc cgtgctgtgt
    14641 gcggcgtcat tgacctccgt ccatgcggcg cagccgatca ctgaaccgga gtttgcctct
    14701 gatattgtcg atcgttatgc cgatcatatt ttttacggca gcggtgccac ggggatggcg
    14761 ctggttgtta tcgacggtaa ccagcgcgtc tttcgcagtt atggcgaaac gcgacctggt
    14821 aataacgttc gcccgcagct cgattcggtc gtgcgtattg cttccctcac caagctaatg
    14881 accagtgaaa tgctggtgaa attgctcgac caggggaccg tgaagttaaa cgatccgcta
    14941 agtaaatatg ctccgccagg cgcacgcgtg ccaacctaca atgggacgcc cattacccta
    15001 gtgaatctgg caacccatac cagcgccctg ccccgtgaac agcccggtgg cgcggcacat
    15061 cgtccggtgt ttgtctggcc aacgcgcgag caacgctgga aatacctttc tacggcgaag
    15121 ctgaaagccg cgccaggcag tcaggcggcg tactctaacc ttgcgttcga cctgctggca
    15181 gatgcactgg cgaatgcgtc cggcaagcct tatacccagt tgtttgaaga gcagattacc
    15241 cgtccattgg ggatgaaaga caccacctac accccctcac cggatcagtg ccgccgttta
    15301 atggttgctg agcgcggtgc cagtccgtgc aataacacgc tggcggcaat tggcagcggc
    15361 ggcgtctatt ccacgcctgg cgatatgatg cgctggatgc agcagtattt atcatctgat
    15421 ttttatcagc gcagtaacca ggcagatcgc atgcagacgt tgatttatca gcgcgcgcag
    15481 tttaccaaag tgattggcat ggatgttccc ggcaaagcgg atgcgcttgg tttaggctgg
    15541 gtatatatgg ccccgaaaga gggtcgtccg gggattattc agaagacagg cggtggtggc
    15601 ggtttcatta cctatatggc gatgatcccg cagaagaata ttggcgcgtt tgtggtggtc
    15661 actcgttcgc cgttgacgcg ctttaaaaat atgagcgatg gcatcaatga tctggtgacc
    15721 gagctaagcg ggaataaacc gttggttatc cctgcatcct gattaatact ctgaaggcaa
    15781 acggttgata gcgaccagtt tgcctttatt catttcgata tagccgcctt tacgcaaagc
    15841 agcgagaact tctgcgacga cagaacgcga tatgcgtgta cgttgatgga tataattcat
    15901 cacaccaata cgtgatcgca attcttcatt ccagtcaatc attgataata atgttgcgcg
    15961 gatttgttcg taggaattat gcccaatgag ctgcacgtcg cgtaattcca gaattctatt
    16021 ttgccaggct aaccagtaaa aagcgtctcg ccagagttga ttttgttcaa taagcgtaat
    16081 ggtttgtttg gctggtagat gatatcccgt acaatttcct tctgatatta atttgtatgg
    16141 tatatcgttt ttcattaaac catcagccag ccccataata taagaagcct gggtaatacc
    16201 gataagtacg ttttcttctc tacgcagaga gataacgccc tctaaaataa cgaaagtatc
    16261 ttcgctattg acatcactgg aaaatataac ttgcttttca ttattaaact cgaagcgcgt
    16321 accgtatctg gacaaacatt tatcgagctt accaaattcc tgaagaggtt taactacaga
    16381 taacatttgc gcgtcctttg cagtaatgcc cgtcaaatcc ttgacgggca ttatttagat
    16441 taaattacca gaatttcttc ggagtgaaga atattaccaa gtatatttaa cacccacgtt
    16501 c
//
LOCUS       DAJTMK010000088        16494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTMK010000088 DAJTMK010000000
VERSION     DAJTMK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16494
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.089"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..601)
                     /gene="capU"
                     /locus_tag="OO135_002415"
     CDS             complement(<1..601)
                     /gene="capU"
                     /locus_tag="OO135_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5398232.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGH"
     gene            complement(604..1446)
                     /locus_tag="OO135_002416"
     CDS             complement(604..1446)
                     /locus_tag="OO135_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5398233.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1632..2828
                     /locus_tag="OO135_002417"
                     /pseudo
     CDS             1632..2828
                     /locus_tag="OO135_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2911..3168
                     /locus_tag="OO135_002418"
     CDS             2911..3168
                     /locus_tag="OO135_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398234.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(3726..4493)
                     /gene="fecE"
                     /locus_tag="OO135_002419"
     CDS             complement(3726..4493)
                     /gene="fecE"
                     /locus_tag="OO135_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5398235.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4494..5450)
                     /gene="fecD"
                     /locus_tag="OO135_002420"
     CDS             complement(4494..5450)
                     /gene="fecD"
                     /locus_tag="OO135_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5398236.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5447..6445)
                     /gene="fecC"
                     /locus_tag="OO135_002421"
     CDS             complement(5447..6445)
                     /gene="fecC"
                     /locus_tag="OO135_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5398237.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6442..7344)
                     /gene="fecB"
                     /locus_tag="OO135_002422"
     CDS             complement(6442..7344)
                     /gene="fecB"
                     /locus_tag="OO135_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5398238.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7389..9713)
                     /gene="fecA"
                     /locus_tag="OO135_002423"
     CDS             complement(7389..9713)
                     /gene="fecA"
                     /locus_tag="OO135_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5398239.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9800..10753)
                     /gene="fecR"
                     /locus_tag="OO135_002424"
     CDS             complement(9800..10753)
                     /gene="fecR"
                     /locus_tag="OO135_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5398240.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10750..11271)
                     /gene="fecI"
                     /locus_tag="OO135_002425"
     CDS             complement(10750..11271)
                     /gene="fecI"
                     /locus_tag="OO135_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5398241.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKAREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11569..11751
                     /locus_tag="OO135_002426"
                     /pseudo
     CDS             11569..11751
                     /locus_tag="OO135_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11745..11849
                     /locus_tag="OO135_002427"
                     /pseudo
     CDS             11745..11849
                     /locus_tag="OO135_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12189..12446
                     /locus_tag="OO135_002428"
     CDS             12189..12446
                     /locus_tag="OO135_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5398242.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(12766..12897)
                     /locus_tag="OO135_002429"
                     /pseudo
     CDS             complement(12766..12897)
                     /locus_tag="OO135_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13179..14537
                     /locus_tag="OO135_002430"
     CDS             13179..14537
                     /locus_tag="OO135_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5398243.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(14776..16161)
                     /locus_tag="OO135_002431"
     CDS             complement(14776..16161)
                     /locus_tag="OO135_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5398244.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(16211..>16494)
                     /gene="tnpB"
                     /locus_tag="OO135_002432"
     CDS             complement(16211..>16494)
                     /gene="tnpB"
                     /locus_tag="OO135_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5398245.1"
                     /translation="FNGLAAKVQTALKDDPMSGHIFIFRGRSGSQVKLLWSTGDGLCL
                     LTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTML"
BASE COUNT         3990 a         4323 c         4365 g         3816 t
ORIGIN      
        1 cgtgcccttt ttccgggcgc aacattccta cctgagcaat tacggggcat cctcttcggg
       61 acgccagcca ggaaagaaca ctcggaggca aactgtttcg ggaatcaacg tataattcct
      121 ggaaatcaaa gccaggaggc acaacagtaa cccgagttcg acatccttcc tgctccagat
      181 gtgtcttcat atttgttccg ggaacaatca cctcatcgca aaaatgattt attgaaaaaa
      241 attttgtttt tcgtgtcaaa tatgtctttt gcctgataat tctgaatgga tgtttccagg
      301 taaaaaaccg taccaaacca acaatattac tatcatgtcc actgtgacaa acgattgcat
      361 cgggctgaaa actatgaact attccgagta atctccatac agtaggaatg tgaagactgt
      421 ttcgaaataa cgcgaatgtg atatcaattc caaacttact ggcttcaaaa gcaattttgc
      481 tgttctccct gcagacaagc agaactgaat gccccatttt cttcagggcc ttcatttgag
      541 caacagcctg taattcctga ccacctatat tcggtgatga ttccgtaaat agtatattca
      601 ttattagtca tccggtaatc ttggcccctt atgaagggcc agcaacgatg aaaaaaacgg
      661 tgttgtataa taaaaaagtc gtcttttcag ccatacgcaa ttttcccgtt ccttggtgct
      721 tattcttcct atccgtgtag ttcctttagc aggagcattc attcttcttt ctgttgtata
      781 taaataatgg aatccaagtt cttcagcgat ctgaatataa tccttgttat aataaccttc
      841 cggccaacac aaatgtttac tgcattttcc tgtcattttt tttaaatatt ctctcccgct
      901 caataagtcc tgccgaagat gtttacactg ttcttctcgt gaagtgaact ttttatccca
      961 acgtgtatga gtatgtgtat gaacatgaaa ttcgaccaga ccagattgca acatttcgtt
     1021 cacttcagac cagcgaagca taacattatc agcattcccg gttgctattt gatgttcgca
     1081 atcgcgatga gaatattcct ttccaggaga gtgacgaacc ggaccatttc caataaaact
     1141 ggtaattaaa aaaatatgtg ccttgagatt aaactcgttt aataaaggat atacctgaaa
     1201 ccagttgtca aggtatccat catcaaatgt caacataacg cttttacggg gaagttttcc
     1261 gccccgataa aaaaactcca gttcatctga tgacagtgtt ttccagttgt tttcagccaa
     1321 ccatttcatt tgtttccgga atgtctcggg agaaagtgta accagtcctg ggcaatggct
     1381 gacatgatga tacattaata ccggaacatg atttgcttta aacaaaatgc cccctccgtt
     1441 taaaatttaa agtttctggc acccagtaag tagagataaa aggaaagcct aataaaggtg
     1501 gtgaacttta tatactacta aatcaaacat caacaattta taaacaataa tatattatca
     1561 tatcttttaa aaacggctca ctaagaacct tgaggtggca cactaaattt agctacctga
     1621 acagaggtga tatgctcacc tcagaacaac acaggtgcca taatgaaaaa agaaatttca
     1681 gcgcagagtt taaacgcgaa tccgctcaac tggtcgttga ccagaaatac accgtggcag
     1741 atgcagccag cgctatggat gtcggccttt ccacaatgac gcgatgggtg aaacaattac
     1801 gtgatgagcg gcagggaaaa acaccaaaag cctcccccat taccccggaa caaattgaaa
     1861 tccgtgagct caggaaaaag ctacaacgta ttgaaatgga aaatgaaata ttaaaaaggc
     1921 taccgcgctc ttgatgtcag actccctgaa cagttttcga taatcgggaa actcagggcg
     1981 cgttatcctg tggccactct ctgccatgtg ttcggggtcc atcgcagcag ctacaaatac
     2041 tggaaaaacc gtcctgaaaa accagacggc agacgggctg tattacgcag ccaggtactt
     2101 gaactgcatg gcatcagcca cggctctgcc ggagcaagaa gcatcgccac aatggcaacc
     2161 cagagaggct accagatggg gcgctggctt gctggcagac tcatgaaaga gctggggctg
     2221 gtcagttgcc agcagccgac tcaccggtat aagcgtggcg gtcatgagca cgttgctatc
     2281 ccgaatcatc ttgagcgaca gttcgccgta acggaaccaa atcaggtgtg gtgcggtgat
     2341 gtgacctata tctggacggg taagcgctgg gcgtacctcg ccgttgttct cgacctgttc
     2401 gcaagaaaac cagtgggctg ggccatctcg ttctcgccgg acagcaggct taccatgaaa
     2461 gcactggaaa tggcatggga aacccgtggt aagcccgtcg gggtgatgtt ccaagcgatc
     2521 aaggcagtca ttatacgagc aggcagttcc ggcagttact gtggcgatac cagatcagac
     2581 agagtatgag ccggcgtgga aactgctggg ataacagccc aatggagcgc ttcttcagga
     2641 gtctgaagaa ctaatgggtg ccagcgacgg gctatgtaag cttcagcgat gcagctcacg
     2701 caataacgga ctatatcgtt ggatattaca gcgcactaag accgcacgaa tataatggtg
     2761 ggttaccacc aaacgaatca gaaaaccgat actggaaaaa ctctaacgcg gaggccagtt
     2821 ttagttgacc acaacagact acctgaaggg agccgcggtc gcctagcagt tgcagtagca
     2881 ggagatcatc cagccgcagt acaggtcacg atgactctgg ttaatgatac cggctttgac
     2941 cccgtatttt ccggctctat cgctgaatca tggcgtcagc agccgtgcac accatcctat
     3001 tgttgtgact gggaggctgc caccatgctt cgcgctttcc ctctggcgaa aaagggagaa
     3061 ggacgggccc gtctgccttc actttatgcc agcttcggta agctgggtga gacaccgact
     3121 catgaagata tcattgataa caatcgatcc atcaactggc ctgtataacg tggctgccgg
     3181 tgattaagaa agctgcacct acctaagtag tagcaaacgc acacttttta gaaaaatcga
     3241 tggtcagaaa ctggattagc aattccgttc cagggttgct tttgatttac gttggcgtct
     3301 gatcattgat ttatcctcaa aagcccaacc tcattggtaa tgaaccagct ccgtgaatgt
     3361 ccgctctggc acagagcgaa attttttgat ctccccccct gaaatctaaa cttagtcatg
     3421 tcacgttttt gggtttctaa aattttaact tcgcgttttt cgttgccgta agggttatac
     3481 agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg atcacgactg gtttgaaagc
     3541 cgcgcccaag cctgtacagc tctggtttgc gttgattatg aacctgtcag cctaaagcaa
     3601 gcggatggac gatgagtatt ggtaatcttt cagagtccgg aaaagttcag ccccagtctg
     3661 aacaggcttg ctggcgccag tccagtttca ttcagtcgtg gtttggttct tacggcctgt
     3721 gcaatctacc tcattaggca catcggcctg ccagataccg gctcggggtg tatttccgct
     3781 tccacgctga atactgttct cagcaatcct ggggtcatca cctcttctgg tgtgccttgc
     3841 gccataacat gtccgtttgc cattaccacc agttgatcgc agtaccggct agcctgatta
     3901 aggtcgtgca gcacagcgac caccgttttc ccctgagtcc ggagttcgcc catcaaccgc
     3961 atcaggtcca cctggtgatt gatatcaaga taggtggttg gctcatcaag taatacaacg
     4021 ggcgtattct gggccaggac catcgccaga aatgcgcgct ggcgctgacc gccggaaagc
     4081 tcggttaacc gacgaacggc aagatgattg atccgggtct ggttcatggc gacattaact
     4141 cgtgcattgt cttcagcgga gagacgcccc cagagtgaca gccagggatt acgaccatac
     4201 gaaaccagct cctggactgt gatcccctct ggcgttaaat ggtgctgagg cagcagcgaa
     4261 agcctgcggg ccaactggcg tgatgagagc atatttatgg gattatcgcc gagaaatacg
     4321 gtgccagact gcggcattaa aagccgcgaa aaacagttta acagcgtcga tttcccgcaa
     4381 ccgttagggc cgatcagggc ggtgatcttc cccgttggca gtgagagtga aacgtcgtta
     4441 agtaccttgt ctgtcccgta actgaccgtc agattttcag ttcgtaaagt catttatcgc
     4501 attctcacaa gcaaccagac aaaccacggc gcaccgataa tggcggtcag cacgccaacc
     4561 gggagctcca gtgggggatg aataattctc gccagcagat cggcaaccac caacagcaac
     4621 gcacctgtca gggctgaaac aggcagcagt ctgcggtgac gtccaccggt gatgctacgc
     4681 atcatatgcg gcaccacgag accaataaag ctaatcgggc cgcaggcggc cacgccggta
     4741 gatgtcatgg cgacagctag taacaaagcc cagaatcggg tatggggcac cgacacaccg
     4801 agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga gatcgcggca aaaactcagg
     4861 ctcagcggca gaaataaaat catcagcggg atggcaatct tcacaaagct ccagtcacgg
     4921 ccccataagc tgccggtcag ccacagcagg gcgttgttca catcctgcgg gcgcgagagc
     4981 atcagataat ccgtcaggct ggcccagcat gcagaaagcg ccacgccggt gagcgccagc
     5041 ttcatcggct ggtgggtctt tgccagcatc ttcagtaata tcaaccccgc catgccgccc
     5101 gcaaaggcca gcagcggcag caccatcacg ggcagtgacg gcataagaag tagagccccc
     5161 acagaggcca ggctggcggc atggttaacg ccgagaatat ccggtgatgc cagagggttg
     5221 cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg ctgcaccgac aaacagtgcc
     5281 agcagcaagc gcggcagtcg gtactccatc aatacataat aatgctcgcg tccggcctgc
     5341 cagtcggtca gcagcgcgcg ccacggcacg gggatcactc ccatatggag tgataacagc
     5401 gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg caattttcat cctcgcctcc
     5461 tcacaagcca gacaaagcaa gggctgccaa tcagcgccag cactgcgcct gcgggcagat
     5521 ctccggggaa ggccagcgcg cgtgcgagta catctgccag cagcatcagc gtggccccca
     5581 gcagcatgct cactggcagt acgttgcgct gatcgaagcc tgcccagaag cgcgccagat
     5641 gtggcaccag cagaccgata aacgccaccg gacctgccac gctgacgcac gcaccaacca
     5701 gaagcagcac taacatattg atgaccaaac gtagcctcgt caggttcact cccagcgtat
     5761 gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt cgccagcagt aacacgacag
     5821 ggactgcagt gaccaccacc ggcaagagct gccagacatc ctgccagcgg gcgtgggaca
     5881 ctccgcctgc cagccagtaa aagatgccgt aagcatgatc ttcggccagc agcagggtga
     5941 tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc cgcgaggatc agtttgtttc
     6001 tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac cagcagccag ctcacgccgc
     6061 ccccgcatgc cgcgatgaac gacagagaat agcctgcaat cggcgtcgga ctcagcgcgc
     6121 tggtaagcgc catagccagc gccgcgccgc tgttaatgcc gagcagtgaa ggagaggcca
     6181 ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag cgccaggctt gcgccgatca
     6241 gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac cagcgcttct ggtagcgttg
     6301 gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc ggaaacagga atggccgagt
     6361 agcaaaacag actcagccag aaaataataa taagtgctgc aacgggaagc ccccacagca
     6421 gcatcgggtg ttttatcgcg gtcatttcac aacggtaagc ggctgatggt ggaagatttt
     6481 taccgtgtca gcggcaatac gctctgcagc aaaaataccg cgcatccgcg cccaggtgtt
     6541 actgtcgacc gaagcaacct gctgcttctg cgcggcggtt aacatctgcc agagcggatc
     6601 ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg gcaaccagca gccaggcagg
     6661 attgaccgcc agcagttgct ccaggccgat ggacggcatg gacgaacccg ccatcgcagc
     6721 gggaacgttc agccccagag aggccagcac gctgccggtc caggtctcct gagtatgcag
     6781 gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt gtccctttgg gaagctggct
     6841 ggcccactgc gccatcctct ctttatgttg ttccagacgt gcctgcatct ctcgcttttt
     6901 acccaccatt tcgccgatga tagccgcaga ttgcaaattt tcagcgtagg tttcgttgcg
     6961 ggacttaagc agcagtaccg gcgcgatttg ctgcaaggcg atgtaaaccc ccgcatggcg
     7021 actgctgtcg gcaatgatca ggtctggttt cagagcggca atggcttcca ggctcggctg
     7081 cgcgcgcgtt ccgacggact gccacggttt caggtgcgca cgcacttcgg gcaggatgcg
     7141 ttttgcatcg ttatcgtcgg caataccgat cgggctgacg tccacggcgg ccagcgcatc
     7201 ggcgaacgag agttccagca ccacaatccg ttgtggcgtt ttttcgagtg taaacgtgcc
     7261 gtgttcgtcc tgaaccatgg cggcaaaggc gtggctgatc accagcagca ggcctgcaaa
     7321 aagaaaacgg ataaatgcca acataatcac attccagcta aaagcccggc aagccgggcg
     7381 ttaacacatc agaacttcaa cgacccctgc atatacagcg tgcgcggctg gcctgcatag
     7441 atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt cctggtcgaa gatgtttttc
     7501 acaccgaatg ccaggttcag atctgccatc tgcgggccaa agtcatacgc cacgcgtgca
     7561 ccccagagca tgaagccggg aatgcggccg gtactgccgt cggcgctctc tttcaccgta
     7621 ttggcgttat ccgcaaactg gctggactgg aaatcgctgt tcagattgaa cgtccagttt
     7681 cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg gggagaatgg taccagattg
     7741 ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat acgcatagct ggcgtagatg
     7801 gaaacgttat caagcgttgg cgttagcgta cccagatcgt aacgtgcctg cgtttccagc
     7861 ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt tggtctggtt ggagtcgtac
     7921 tgattgttaa agttaatcag gaacagcccc atttccgccg tcagcgcgcc gtcgtcgtag
     7981 cgggtaccga gttcccaggt tcgcgctttt tccggttcca cattgccgct ttgcacagcc
     8041 ttgccaatct ggctgtactg tacggtgccg aacgagcctt cagtgtttgc ataaagattc
     8101 cagctgtcag tcaggtgata gagcacgttc aacgccggaa gcggtgcgtt atagctcact
     8161 tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact cgatatgttc gaaacgcata
     8221 cccggcgtga tggtccagtt gccgatgtcg attttgtcat ccagatacca ggcgtgcgcc
     8281 tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc cggacggcaa ctgcccgctg
     8341 ctggtggcgg tgtagtaacg catttcatgc gttgattcat tcacatagcg atagcccacg
     8401 cccacttcgt gcgcggaagg gccgatcata aagatctggc tgtagcgtgg ctcaataccg
     8461 cgcacccagt agttacgcgg cgagagggtg atgcgtttgc cttgctccag gtagccgctg
     8521 cgcagggttt gggtgtagaa cccctgaatg ttgaatttat gctggctgtc tggctggaac
     8581 tggtagccca ggctcgccag cttgcgacga ccccagaagc ggtcatacgg gcgggtggat
     8641 tgccagcgat cggcgtcgta atccgcgcga gacaggccac cgggcatgtc ggcttcaccg
     8701 tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat ccggcgcata tttgcttttc
     8761 agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc agtcactgcc gcgcgtgccg
     8821 gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc cgcccaccat caggttgtgc
     8881 gtctctttcg ggttgttttg tgaagaggtt gggctgagct gaccttccac gcccgcctcg
     8941 ataccaaagt cctgcggaat ggcacgggta acaaagttca ccacgccgcc cacgctctgc
     9001 ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa tggcatccat gttgccgagc
     9061 gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg cgaaggggac ggggatgccg
     9121 tccatcagga cggtcgagcg gctggcgagg cgcgggttca ggccccggat gccaaagttc
     9181 atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg cgctgacgcc agggatgcgg
     9241 ttaagtacct cacgcatggt ggttgcgccg gttttggcga aatcctcacg gcggatcacg
     9301 tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg catcacccag ccagtcgccg
     9361 accacggtca gggcatcttc ttttggtgcg ggcgcgggct ccagcgtcca gctgttattt
     9421 cccagcggtt ttacctgcag tccgctgccg tccagcagtt gttgcaggcc gctctcgacg
     9481 tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg tcaggctggc gtcaaccgag
     9541 agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt tatcgagcga tcccggtgcg
     9601 atattaacct gtgcagcaaa agcggaaaac gagagaccgg ccagcggcag caggctcagg
     9661 cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc gtaacggcgt cataccttcc
     9721 ccatcatcat ttttgttgtg ttcagctatg agtcgaacga gaagggtaaa aaggacaatc
     9781 gaaataagaa ttattttcct tacagtggtg aaatgtttat ccagtaccgc gtaatagact
     9841 gaattttaac gggaagcgtt tgcgcgataa cgttcaggat cgcatcggta tttttcagcg
     9901 ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc gcagcgcagc acgccgttgc
     9961 ggtaacgggt tagcgtggct atcacctcac ccagcggttt atcgctgaag ctcaggatgt
    10021 ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac tgcgccaaac tcagaggcgc
    10081 tgaactgcag gctttcacca gcgttcacga tgcgtttttg cgcgggggca ctggcgagaa
    10141 gcacttccac agcgtgctgc tgaacgtcaa gctgcgtgaa attatcctgc tggcggacgg
    10201 taaattctgt ccctaaagca gtgagctggc cctgacgggt caggacacgg aaggggcgtt
    10261 gcagggcatc tttcgcggta gtaatggcga tttcaccgta ccagagccgg acggtgcgct
    10321 gatgcgcatc aaaacgcaca tccgccgcgc tttgggtatt cagcgtgagc agggagccat
    10381 cttccagttg ctgacggctg actgtgcctt tggcggtgcg gtaatctgcc cgcagacctt
    10441 cgccggtttc cgactgccag agctgccagc ctccgccagc gccgagcaac agcagtaatc
    10501 ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa cgcccggctg gcaacgtcgc
    10561 caggcacacc gccaagctgg ttgcgcaggt tttcaacctg ctgccaggcc cactggttat
    10621 cctgatcctg ttcataccac tgttgccagc gcgcttcctg ttgtgggctg acgcgctcgc
    10681 cgcttagcac ggcataccag tgggaagctg aacgcagcgc ctgacggcgg gaatcggtta
    10741 acaaaggatt cataacccat actccagacg gaacagcagg cagtgctcga cggctttcgc
    10801 cacgtatttt ttcacggagc tgatggaaac accgagtttg tgcgcaatct cgctgtatgt
    10861 cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgct ttgccgttta gcccgtccag
    10921 catgctgtcg aggagttgta gggtctcgag ttggctttcg cgttcctcag gtgaaggcgc
    10981 tcccccctcc ggcataagcg ccagcatctc cagatacgct ttttccagcg cgtttcggcg
    11041 aaacaggtcc accatcacgc gtttggcgat agtgcagagg aaggagcgag gatcgcggat
    11101 cgtcgagagc gtttcgctga ccattacccg caaaaaagtg tcctgggcaa tgtcatctgc
    11161 atcaaaagca gactggagtt tgcgcgtcag ccagcttttc aaccagccgt gatgtgtgcc
    11221 ataaagcgac tcgaacgtta aggaagctgt ggtagtggcg cggtcagaca tgcggagtgc
    11281 atcaaaagtt aattatcacg tagtcatatt aatatgagaa tggttatcat tacaattgga
    11341 aataaaattg tttccaatag acatttttaa catgttgttt ttctaagtgt tataaggtag
    11401 gtataaaatg ggatggagcc tctgcttctg acatgtgtcg gtcagaatga ctcatgatgt
    11461 ggtctgctat tattgacatc ctcactgccc taaaggatgg ggatttcggt aatgatctta
    11521 cccagcaata gtggacacgc ggctaagtga gtaaactctc agtcagaggt gactcacaag
    11581 acaaaacagt ttcaaccagt aaaaaaacgt aaacaccatt cgcatgaatt tcgtagttaa
    11641 gatctgaagc ttgcagaatg catcggtgtt actgccggag tccgtgaact tagtctgcat
    11701 gaatcaccac agctttacat ctgtcgcagt aaacagcaaa atcagcaaac ttacttaatt
    11761 atatcgagtg cgattacaat cgctggcgat ggtacagttg gtgtggcggt ctcagtccgg
    11821 aacaatttga aaatcataat ctcacttaaa acggcatcca ttttacaaaa gatggccgtc
    11881 tgtgtatttg taatgaatac cttctacatg tttagagtgc ataatccacg acaaggcgat
    11941 atcctaaaaa ttagctagca ggcaactttg atggcataga taatgttttt agtaaaaaag
    12001 agttgactga atataataca gcctcttcct acataagaag gaatgtgctc acaaaaaaaa
    12061 accattagtc tgcttcctgt tcttacaata atcagtatta tgtattttat ttatatagca
    12121 aaatatgctt gttacacctg tcataattag gcatggtaaa attattgact gaaccctggg
    12181 ggagaaagat gcacagtgat tattataata tggtttttgg tgaaaaattg gccaatattt
    12241 tatatgaagc taactctcaa tttttttacg agagaaatgt catagaagag gcagtcaacg
    12301 ctttattctg tgagcgtgaa ataataaaca acaagaacat aataaagaag ctgatgttct
    12361 ttttgtcaga tgttaatcac accaagaaag atgttgttca gagcgcactt aatattatca
    12421 ttgacattac atcaggtgat atttgagatt ttacataaaa gataatctct caaggagtct
    12481 tatctgaaaa cgaaatatat agtccctctc gttttgtaat atcgattggc tttgttgaat
    12541 cggttacgta ccccggagcc ggtcatattt tcaggtaata cacacgcttt cagacatttc
    12601 ggctttcgtc atttttgtat catgggcata gcttctgcta cctgaccatc ataatcacgc
    12661 agcttcagtg aagcagtgac cacctgttta acccggtgta tcgttatgat cttatccagc
    12721 aattgaaccg ccccgggttt cctggagagt gttttatctg tgaactcagg ctgccagatc
    12781 atcgtttccg atggaagcat aataagcttt ttctgcttct gccggtggga tatggccctg
    12841 cctttccagc aatcgtcgat tgttatacca gtccacccac gtgagtgtgg tcagctcatc
    12901 atactgacac gtgcattacc cctaccgcga taatgcaacc gtacaatata gggcgtttcc
    12961 taaacgcagc cctcatcact ccaatccaac aagacacagc atttttgatc acgaagaaag
    13021 acaaataatt tccgtaaatg tttccgttgt tacaaataac aacagtgttt tacatttctt
    13081 tggcctcagg cccgcgcatg ttgccattcg atgtcatttt cctgactcgt ttcttcttta
    13141 tggtgtagtg tgatcttcac tttatagatg ctaacaatat gaaaagaaaa attattcccg
    13201 tgctaatcgg ttgtacgctc tctttctctg ccctggcggc acaacctacg gctgagcgct
    13261 atgtcgtcag ttttccagaa ggcacgcacg tgaactacgc cggcgcattt gccagtgcat
    13321 tcccgaacgg ccttccggtt gggataggtt ctggcctgct gttcaccggt aaacagggcg
    13381 atgccctgac attcgcaacc attaccgatc gcggtccgaa cgcggattca ccgaaagagg
    13441 ggaaaaacga aactaaaatt ttcgttacgc cagatttcgc gccgctgctg atgacgattc
    13501 gtgtacaaaa cggcaaagcg gaggccatcg atccccgtcc tctgcatgac gataaaggcg
    13561 cgatcaacgg tctgccgctg gcaagcgacg tgattggctc caccaatgaa gtggccttca
    13621 gcgacacgtt acatagactg aagggggata atcgcgggct ggatacggaa gggatcactc
    13681 cggatggtaa aggaggttac tggctgtgcg atgagtatgg cccgttcctg attaacatcg
    13741 acagtaaagg gaaaatcctg gcaatccacg gtccgcaggc ggcggaaggg gaaaaagcga
    13801 ttgcgggcgg tttgccaaat atcctcaaat ggcgtcaggc gaaccgcggc tttgaaggcc
    13861 tcacccgtat gccggacggg cgtattattg tcgccgtgca aagtacgctg gatatcgacg
    13921 ctaagagcaa gaaaaaggcg ctgtttacgc gtctggtgag cttcgatccg gcaagcggaa
    13981 aaaccgcgat gtacggttat cctatcgaca gcgcggccta cagcaaaaac agcgacgcta
    14041 aaatcggaga catcgtggcg ctcgataacc agcacatcct gctgatcgaa cagggaagag
    14101 ataaaaacaa cagaatgcgt aacctgattt acgaggtcga tctcaataag gcgagcgacc
    14161 tgtcaggctt cgacaagccg ggtgagtatc cggagtttga tgatgagaaa accctgtcac
    14221 agcgcggcat tacccttgcg cagaaaacgc aggttgttga tctgcgctcc ttgggctggc
    14281 agcaggagaa ggctgaaggg ttggcgctga tcgacagcaa aacgctggcg gtggcgaacg
    14341 ataacgactt tggcgtgaag gtggcgatgc aacatccggt cgagggcaag acattcaagg
    14401 attatcgggt gaacgcggaa ggaaagctga cgcttgacga caagcaggtt gaaacgaccc
    14461 tcagagtgaa gccactggag aaaccggagt ctgacagtga actgtggatt gtgacgctgc
    14521 cggaagcgtt gaaataagtt tataaggtac ggacagcagt atcgcttagt ttttcaaatt
    14581 catgtatgtg aacaatattg gggttccggc gtagaccccc aatgaaagcg tcaccacgtg
    14641 aaaacatgcc tgcacggttt acacgtcatc ccatggtggc agccggttaa ctttcctgcg
    14701 ccaccagtgg aaaaaggcca taagcagtcc gaacaaaagt aagcgtacag cgagggccgt
    14761 attgacgggg atgtgttatt cagctggcag tgctatgcgc cacggaagca gttcgctgac
    14821 ccggttgacc ggccagtctg ctatgacgcc aagcacatgg cgaaggtagc tttctggatc
    14881 cacgtcattc agtttgcacg tcccgatcag gctgtacagt agcgctcccc gctcaccacc
    14941 atggccagag ccgaagaaca gggtcttcat cttttcacgc aaccagcttt ccagggattt
    15001 caacagcggt ttcgtttttc gctgacgttc agcaagccgc tgctctgccg gcattcccct
    15061 tatatccgcc tctatggcgt acaactgacc gatctgctcc agggcttctt ccgtcagtgc
    15121 tgacgggatg cggacgtgca catcgtggat ctttcggcgg gcatgagccc agcaggcagc
    15181 ttccgttatc ccaccattgc gatacagctc gttgaacccg gcgtacgcat ccgcttgcag
    15241 cacaccgctg aagcaagcaa gatgagtctg cggatggatg ctttttctgt ccgggctgta
    15301 agcgaaccac actgcaggtg ccaacgctga cccggcattg cggtcatcac gaacatacgc
    15361 ccacaaccgc ccggtcttcg tcttcttatt acccggcagc agtacctgga ccggggtatc
    15421 atcggcatgg agtttgccgt cagtcatgac atagccatga agcgcctctt ccagcggaga
    15481 cagcagccgg cagcatgcat ccacccagcc cgacagcagt gaacggctca gctccacacc
    15541 ttgccggccg tatatttctg actggcaata cagcggggtg tgctctgcat acttcgaggt
    15601 cagcacgcgg gccagcagcc ccggtccggc gataccccgc tcgatgggcc gcgaaggtgc
    15661 aggtgcctgc acgatggtat cgcactgagt acaggcatgt ttttcccgta ccgtccggat
    15721 aacccggaag gcgctacgca tcaactccag ctgttcggcg gtatcctcgc ccagatagct
    15781 cagtgaaccg ccgcagttcg ggcagcacgg cgccgcaggc aacagtcgct tttcgtcacg
    15841 gggtagtgat tcagggaacg gcttacgggt gcgggtctga cgcaacggac gctgtactgc
    15901 cgggtcatac accctaccag tcagcgtatc gctctctttc tgaagccggt tcagatcggc
    15961 ttccatttgt gcgatacggc gggagacttt ttcggaacga ctgccgaagt tcatccggcg
    16021 gagtttatcc agctgcgcct gcagatggtc tatttcgcgc tcccggttgc tcagcttttc
    16081 ctgcagggcg tggatcagcg cttcctgttc ggccaggcgc tgtttcagca ggaagatgtc
    16141 gtcagaagag atgtcgttca taagcccgta ttttaccggg cttattctgt gacaaccagg
    16201 ataaagagat ttacagcatg gtcagggagg tcagcagccg cttgggctgt cgccagtcga
    16261 taccttccag cagcatcgcc agctgcgcct gcgtaaggaa cactttgcca tcacgggctg
    16321 acggccaggc gaagcgccca cgctccagcc gtttggtcag gaggcacagt ccgtcgccgg
    16381 tggaccacag cagtttaacc tgactgccgc tgcggccccg gaaaatgaaa atatggccgg
    16441 acatgggatc gtctttcagc gccgtttgta ctttcgcagc caggccgttg aagc
//
LOCUS       DAJTMK010000089        16471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTMK010000089 DAJTMK010000000
VERSION     DAJTMK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16471
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.090"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1561)
                     /locus_tag="OO135_002433"
     CDS             complement(<1..1561)
                     /locus_tag="OO135_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5398246.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFNMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVLYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
     gene            complement(1579..3495)
                     /gene="mngA"
                     /locus_tag="OO135_002434"
     CDS             complement(1579..3495)
                     /gene="mngA"
                     /locus_tag="OO135_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5398247.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKQALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            3663..4385
                     /gene="mngR"
                     /locus_tag="OO135_002435"
     CDS             3663..4385
                     /gene="mngR"
                     /locus_tag="OO135_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5398248.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFLDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(4489..5358)
                     /gene="sucD"
                     /locus_tag="OO135_002436"
     CDS             complement(4489..5358)
                     /gene="sucD"
                     /locus_tag="OO135_002436"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5398249.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(5358..6524)
                     /gene="sucC"
                     /locus_tag="OO135_002437"
     CDS             complement(5358..6524)
                     /gene="sucC"
                     /locus_tag="OO135_002437"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5398250.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(6800..8017)
                     /gene="odhB"
                     /locus_tag="OO135_002438"
     CDS             complement(6800..8017)
                     /gene="odhB"
                     /locus_tag="OO135_002438"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5398251.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLEKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(8032..10833)
                     /gene="sucA"
                     /locus_tag="OO135_002439"
     CDS             complement(8032..10833)
                     /gene="sucA"
                     /locus_tag="OO135_002439"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5398252.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(11134..11850)
                     /gene="sdhB"
                     /locus_tag="OO135_002440"
     CDS             complement(11134..11850)
                     /gene="sdhB"
                     /locus_tag="OO135_002440"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5398253.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(11866..13632)
                     /gene="sdhA"
                     /locus_tag="OO135_002441"
     CDS             complement(11866..13632)
                     /gene="sdhA"
                     /locus_tag="OO135_002441"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5398254.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(13632..13979)
                     /gene="sdhD"
                     /locus_tag="OO135_002442"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(13632..13979)
                     /gene="sdhD"
                     /locus_tag="OO135_002442"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5398255.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(13973..14377)
                     /gene="sdhC"
                     /locus_tag="OO135_002443"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(13973..14377)
                     /gene="sdhC"
                     /locus_tag="OO135_002443"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5398256.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            14758..14865
                     /locus_tag="OO135_002444"
     CDS             14758..14865
                     /locus_tag="OO135_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398257.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            15071..16354
                     /gene="gltA"
                     /locus_tag="OO135_002445"
     CDS             15071..16354
                     /gene="gltA"
                     /locus_tag="OO135_002445"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5398258.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         3898 a         4484 c         4132 g         3957 t
ORIGIN      
        1 gcaatactcg gtcatagcgc acaccgctgt ctttatctac cagttgcaga gaaccatcct
       61 cattgagcgc aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg
      121 caatcacgtt gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga
      181 caatctggtt gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta
      241 tttgccgatc gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacagtacag
      301 gctgaccgcg atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttaa
      361 taacttcttc ccgcggccag ggcatcaggt taaacagcac gagtttgtcg gcatcactct
      421 gtggcatgtt atcggcaatt ttgcgcatgt tgaaacgcag cagattatcc gccatgtctt
      481 cagccagttc gaagcgggcg acaatttcgc gatgaacttt gtcactgcaa caacaaccga
      541 tactgtcgtg ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt
      601 ggtattcaaa acccaacgtc caggccagcg ttgccagcgg ttccagcaga ttaacaatct
      661 tattttcaat acgtgcgtgg gcaattttga tatccatgcg cgtggaaccg atggtgcgat
      721 gtacgcgcat atatttgcca tcgataaatt cacctttcag ggtggccaga ctttcacgct
      781 gcgcttcgat cttctcaaat acctcttcaa agcggctcat cacaaactta cgttgagggt
      841 agatctcacg tagcttatcc atcacttcga agatattttg ctgcaatggc atctggtcat
      901 gaccattcgg cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac
      961 tgtcgaggcg tttacgtaat ccgttttcgt cggcaggtaa gtacttaccg atggcgtagc
     1021 ccagcggcag cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg
     1081 ttttatcagt accgtggcgc tccgaacatc cgcgccagaa catggtgcgg gtaatgccaa
     1141 atccattgta gatatgcggc agttgcccgg acatgccaaa ggaatctggt aaataaccta
     1201 ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga
     1261 tggattccgc agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact
     1321 tgccggcttc tacctgtttc ttcacacggt ctttgttttc cggtttcacc gcgaaataat
     1381 cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca
     1441 gtcggcacag gatctcttcc atattattga ccagcagaat acgtgactct tcggtggtga
     1501 aataccactc tcgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca
     1561 tcgtcgtttc ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac
     1621 cgcgtgacgc cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc
     1681 accaaaccag ccaattgcag ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag
     1741 cgagaaaatg ccggcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc
     1801 cgttaccata gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat
     1861 cgggatcgcc ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc
     1921 aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc
     1981 cgaaaccatt ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc
     2041 gcccaggcag aatgcataag cggctttgtt cactggccct ccaaggtcaa aggaacacat
     2101 aaaaccgaaa atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc
     2161 ggtaagcgag ttattgatcc atgcgacagg ttctcccacc acaaacagca tcagactgcc
     2221 cgctcccaac gtaccgagca ccgggtagag ataaaaagtc aggaatccat tgaatttact
     2281 gctaagacgc aagtgatttt tcacccagcg catcaagtaa ccggctatca atccgccaac
     2341 gaccgcgccg agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg
     2401 cgctaacgcc tgtttatctg ccagagaata ggcggtatag gccgcgagca ccggtaccat
     2461 caaaattccg agcagcccgc cgcccagctt gcggtacatc cacagccagg agttttcttc
     2521 attaaacaga tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt
     2581 gccccccgcg acaatcaacg gtacggcaaa agagattccg ctcaacagtg cctgtttcag
     2641 ctccgttttg acacttttca ccggttgcgt atcttgctgt acggtacgcg tctcatcgct
     2701 acgcttgagg gtaagcgctt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac
     2761 aggcactgaa agcgcgggaa tgccattaaa acgctcactc tccttgatgg cgacttcagc
     2821 cgcaaaaata caggcggttg cactattgag ttgatccgcc gttaaacgcc cttcaatgcc
     2881 gttagcgcct tgtttttcaa cgtaaacatt tacgccgagt ttgcgtccgg ctttttccag
     2941 atattccgca gccatatagg tgtgagcaat acccgccgga caggccgtta cgcagacgat
     3001 agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga
     3061 gagcaactca tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgcaag
     3121 gcgcgtagtc agcgctgtca gcagttgcat atgcatagta cccgcttcat tggggggaat
     3181 cgccagcagc accactaaat caactgcttc cgggccatca acgccttccc actgaagcgg
     3241 ctcgctgagt gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg
     3301 cacagccaac ccttcaccta aggcggtcgg gccaaggctt tcacggcgat acacttcttc
     3361 cagaaattgc tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg
     3421 gatggcctct tcacggctgg taaagcgcgc attcagacgc aacgcatcgc ggtgggtcag
     3481 agtcgtcaga ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg
     3541 atgaacaata ccattatatg atcaatttca cttgattgaa atcatttgta ttgtatttgc
     3601 attatttaca ggatgaattt atcgggcata atggctaaat tcgtattaat gagatactaa
     3661 aaatgggaca caagccctta taccggcaga ttgccgatcg cattcgtgaa caaattgcgc
     3721 gtggcgagct taaacctggc gatgcgttac ccactgaatc agcgctacaa acagagtttg
     3781 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagct agtcgagcag cagatcctcg
     3841 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
     3901 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagtcc
     3961 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgcgc
     4021 aggatcgtgt gtggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
     4081 aagaaacctg gatgccgctt gcgttgttcc tggatctcac ctggcaggtc atggaaaatt
     4141 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
     4201 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
     4261 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
     4321 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
     4381 gataagcaca aaaaaggcca tcatacgatg gccaaccatg tcgaaaccga cagcccttcg
     4441 ccattcctgt agagggaagc ggcgagggct atttcttatt acagatattt atttcagaac
     4501 agttttcagt gcttcaccga tatccgccag gctgcgaacg gttttcacgc ctgcggcttc
     4561 cagagcagcg aatttctcat ccgcagtccc tttcccaccg gcaatgatcg cacccgcgtg
     4621 gcccatacgt ttgcctttcg gcgcagtcac accagcgatg taacctacaa ctggcttggt
     4681 aacgtgctct ttgatgtacg ctgccgcttc ttcttcagcg ctaccgccga tctcaccgat
     4741 catcacgatc gcttcggtct gcggatcttt ttcgaacatt tcgaggatgt cgataaagtt
     4801 agagcctggg atcgggtcac cgccgatacc aacacaggtc gactgaccga aaccgtaatc
     4861 cgtggtctgt ttaaccgctt cataggtcag tgtaccggaa cgggaaacga tacccacttt
     4921 acccggtttg tgaatgtgac ccggctggat accgattttg cattcacccg gagtgataac
     4981 gcccgggcag ttcgggccga tcatacgaac gccggcttcg tccagtttca ctttcacagt
     5041 cagcatatcc agcgtcggga tgccttcagt gatggtgata atcagtttga tgcctgcatc
     5101 aatagcttcc agaatggagt ctttgcagaa cggtgctggt acgtagataa cagaagcggt
     5161 agcgccagta gcagcaacag cttcacgcac ggtgttgaac accggcaggc cgaggtgggt
     5221 agtaccgcct ttacctgggg ttacgccgcc aaccatttta gtgccgtatg caatggcctg
     5281 ttctgagtgg aaagtcccct ggctaccagt aaagccctgg cagataacct tggtgttttt
     5341 atcaattaaa atggacatta tttcccctcc actgcggcaa caacctgctg agctgcatcc
     5401 gtcagacctt ttgctgcaat aatattcagg ccgctgtcag ccagtttctt cgcgccgagt
     5461 tcggcgttgt taccttccag acgtaccacg accggtacgt taacacccac ttctgctacc
     5521 gcgccgatga taccgtcagc gatcaggtcg caacgaacga taccgccgaa gatgttaacc
     5581 agaacgactt tcactttgtc gtcagagagg atgattttga acgcttcggt tacacgttct
     5641 ttggttgcgc cgccgccaac gtcaaggaag ttagccggtt cgccgccgtg cagtttaacg
     5701 atgtccatcg tacccatcgc cagacctgcg ccgttaacca tacaaccgat gttaccgtcc
     5761 agcgcaacgt agttcagttc ccactgtgca gcctgtgctt cacgcggatc ttcctgcgac
     5821 tggtcacgca tttcgcgcag atcaagctgg cggaacagtg cgttgccatc agcgcccagt
     5881 ttgccgtcga ggcaaatcag atcaccctgt ttggtgatga ccagcgggtt gatttcgatc
     5941 aacgccaggt cgcgctccag gaaaatggtc gccagaccca tgaagatttt ggtgaactgc
     6001 tgaaccagtt taccttccag acccagtttg aacgccagct cgcgtccctg atacggcatc
     6061 gggcctgtca gcggatcaag cgcaacttta tggatcagct gcggagtttc ttccgccact
     6121 ttttcgattt ccacgccgcc ttcagtggag gccataaaga ccacacgacg ggaactacgg
     6181 tctacaacgg caccgagata cagctcttta gcgatatcgg tcgctgcttc aaccagaatc
     6241 tggttaaccg gttggccatt ggcatctgtt tgatacgtta ccagacgctt gccgagccaa
     6301 ttttctgcaa aagcacggat gtcttctttg ctgtttacaa ctttcacacc gcccgcttta
     6361 ccgcggccac cagcgtgaac ctgacatttc actacccacg gaccggcacc gatttttgaa
     6421 gcggcttctt ctgcttcgcg cggagtagta caggcataac ccaccggtgc tggtaagcca
     6481 tagcgggcaa aaagttgttt tgcctgatat tcatgtaagt tcatgtgttc tgtccatcct
     6541 tcagtaatcg ttatctttta aaccgtagac cgaatatttc gtatcgcttc cgggcattgt
     6601 cggtcacctg taggcctgat aagacgcgta agtgtcgcat caggcatcgc ttgtcggatg
     6661 cggcgcaagc gccttatccg acctacattg gtgcttcccg taggcctgat aagacgcgtt
     6721 aagcgtcgca tcaggcttca attgccggag aaggcgataa tgccttatcc tgtctacagt
     6781 gcaggtgaaa cttaaactac tacacgtcca gcagcagacg cgtcggatct tccagcaact
     6841 ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg tgatcgtagg
     6901 acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc atcggacgat
     6961 ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc ggcgtagaca
     7021 tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc agatcttcaa
     7081 cggtcagctt gccatcacgg cctttgactg ccagctcttt gattttcttc tcgatgtctg
     7141 ccatgccgag ggtatcgaca tcacgcagaa ccggcgtcac caggccgcgc ggcgtagaaa
     7201 ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg atagaagcat
     7261 tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag gacataaagc
     7321 ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc agatccataa
     7381 tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt ttcgcttcca
     7441 gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt tcactacgtg
     7501 cagccagagc cggttgcgcc gccggagcag ccgctgccgg tgcagactct ttcgccgggg
     7561 atttctccag atgtttttcc acatcttcac gggtcagacg accacccacg ccggtgcctt
     7621 taatagcgct ggcgtcgagg ttatgttcag ccagcaagcg acggatcgcc gggcttaacg
     7681 catcgttgtt ttgctcttcc agagacgcct gctggcgttg cgccggagtg gacgctttct
     7741 cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg cgaccaagga
     7801 tctgacgaga cgttacggtt gtaccttcat cttccagaac cgcatccaga atgccgtctg
     7861 ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc acttcatcac
     7921 gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg gctacggatt
     7981 caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta tttattcgac
     8041 gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca tataccctac
     8101 cgccggagag gcggaggccg ggcggcctgc ataacgcaga gaagccccaa acggaatcac
     8161 ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct cttcctggca
     8221 ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg ctttatgcgg
     8281 gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt tcttacgacg
     8341 ctgttccagc aggtcgtaat aaaccttacc agaacacatc actacgcgct tcacgccctt
     8401 cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg ccagttcttc
     8461 gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca cgaccagcgg
     8521 acgacgcatc ccgcgcagcg cctgacgacg aagcatgtgg taaacctgtg ccggggtaga
     8581 cggtacgcac acctgcatgt tttgctcagc acaaagttgc agataacgtt ccagacgcgc
     8641 ggaggagtgc tccggcccct gcccttcata accgtgcggc agcaacatca ccagaccaca
     8701 catccggccc catttctgtt cgccagagga gatgaactgg tcgataacca cctgagcacc
     8761 gttagcgaag tcaccgaact gcgcttccca gatggtcaga gtgcgtggtt ctgcggtggc
     8821 ataaccgtat tcaaacgcca gcactgcttc ttcagacagt acggagtccc agacgcggaa
     8881 cgcgccctgc ccgttatgga tgtgttgcag cggcgtgtaa gtggaaccgt tagactggtt
     8941 gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac ccgacaggcg
     9001 aaccggaatg ccttcgtcaa ccagcgtggc gtaagcgagg ttttccgcac cgccccagtc
     9061 gaacagtttc tcaccggcag ccatcgcctg gcgatcgcca taaatcttgg caacgcgaga
     9121 ctgcatttca actgcttccg gcaccgtgct gatgcgtttc gccagctcct gcaggcgctt
     9181 catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg gcgaccaggt
     9241 gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag catccagcgc
     9301 atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgccactt tttcctgctc
     9361 cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct gatacatcag
     9421 cggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt agcacaccag
     9481 gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac gggtcacaaa
     9541 ggcaacggct tccggatcgt ccgcgttaac gtggaaaatc ggggcctgaa ccatcttacc
     9601 gatatcagta cagtacggcg tagaacgggc atccagcgga ttagaggtgg tgaaaccaac
     9661 ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg ctttcgacat
     9721 gttcagggtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt ggatggtgat
     9781 tggcagcact ttgttgctgc tcggctcatc aagtctgtcc agacgggcac gaacagaacc
     9841 gataactacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg ccaggtgcac
     9901 caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca cgtcacccgt
     9961 gccgaggtgt tctttatgtt taccggcgaa ctcgtcgaac aagtcttgcg gttttttacc
    10021 cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccgagaa ccacttcgcg
    10081 ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta acgcgtcacc
    10141 gccttccagc gagaagcgtt ttgcgccagg gaattttgcg ccgaggtaac gttcaaggcc
    10201 ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcgctattga aagtcgcgcg
    10261 accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtga tgtgcatata
    10321 ctcggcacca atcgggccgc agtaggtttg cttgagggct tccagcagct cgccgagttt
    10381 catggtttct ttgccgctgg caaatgaacc gacgttgaag gtttcctgga agtctgcttc
    10441 ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc acagtcccag
    10501 cggatcgaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga gctgcaggac
    10561 tttaacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac gtgaagcgtc
    10621 tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg gtttgactcc
    10681 cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt caggatcggt
    10741 taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga ggtaagaaga
    10801 gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat cttttttatg
    10861 cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt cacttgcgag
    10921 ctcttgtcta cgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt gtcaggggca
    10981 gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt atcaggccta
    11041 cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact ggttgcctga
    11101 tgcgacgctt gcgcgtctta tcaggcctac ggtttacgca ttacgttgca acaacatcga
    11161 cttgatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac tgacgcagtt
    11221 catgatgctg tgacagcgga atacgctgaa tgcatcgctc aaaccgtcga ggcggctgtc
    11281 agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc
    11341 gataaactta tcgggattcc accagaaaga cggacaagag gttgaacaac atgcgcagag
    11401 aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta aatgctcgcg
    11461 agctggcgga ttttgtccat tattcaacag gtaaggctta attttctcat attgcgcata
    11521 gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat
    11581 cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt
    11641 cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacgacagg agcggcggaa
    11701 cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca tcatgtcgcg
    11761 accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa catccgggtt
    11821 atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt aagtacgaat
    11881 cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga
    11941 ttccgactct ggcagataca gggagtggca cagccagttt tcatcatcac gatccgggaa
    12001 gtcgaagcgg ctatgcgcgc cacggctttc ggtacggaag ttggcagaaa cagccgttgc
    12061 atacgccgtt tccatcaggt tatccagttc caggcactca acgcgctggg tgttgaactc
    12121 gctggaagtg tcatccagac gggcattttt cagacgctcg cggataactt tcaactgctc
    12181 aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct gcatacattc
    12241 ttgcagcgct ttacgaatcg ccaccggatc ttcaccgtta cgattattgt tccagcggtt
    12301 cagacgatcc agagatgctt caacatcaga ctcgctggca tcgcgcaatg cgccctgctc
    12361 ggcgatagac tcttgcagat gcagacctgc cgcacgacca aagaccacca ggtccagcag
    12421 cgagttgccg cccagacggt tagcgccgtg taccgataca caagcgattt caccaacggc
    12481 aaacagtccc ggaacaacca catcttcgcc tttctcattc acagtcagcg cctgaccggt
    12541 cactttggtc ggaataccgc ccatcatgta atgacaggtt gggataaccg ggatcggctc
    12601 tttcaccgga tcgacgtgag cgaaggtacg ggagagttcc aggatgcccg gcagacggga
    12661 ttcgagaact tctttaccca ggtggtcgag tttcagtttc gcgtgtggcc cccacggacc
    12721 atcacagccg cgaccttcac ggatttcgat catgatggaa cgcgccacca catcacggcc
    12781 cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat gtttgttcag
    12841 cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac cggcaatacc
    12901 ggtcggatgg aactgccaca tttccatatc ctgcaccggt acgcctgcgc ggatagccat
    12961 gccgacgccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa tacgccctgc
    13021 cccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac cagtttcgat
    13081 gcacagtgca gtacaaccca ccaccgcgcc atcctggttc ttcaccagat ccagcgcata
    13141 ccactcggag aaaatggtgg tgtggttttt caggttctgc tgataaagcg tgtgcaacag
    13201 tgcgtgaccg gtacggtcag ccgccgccgc agtgcgtgcc gcctgctcgc cgccgaagtt
    13261 tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcaagac gcgagaacgg
    13321 caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca tatattcaat
    13381 cgcatcctgg tcaccgatat agtccgaccc ttttacggtg tcgtacatat gccattccca
    13441 gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg
    13501 ggaacgggtc gggaagactt tagagagcag cgcacaggtc tggccgctct gggaaatttg
    13561 cagcgccgcg cgcatacctg cgccaccggc accaatcaca actgcatcaa attctctgac
    13621 tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta aaccaccagt
    13681 gcaacgacaa tcaccagttg cagcatcagg cgcaatgcca gcggtttaac gtagtcggtc
    13741 aacacctgcc acatgccgat ccaggcatga atcaagatag aaaacagcgc cagcagggtg
    13801 aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt cagttcgcca
    13861 ctggtggcaa aaaaaccgac catataaatg atgtagagcg tcaggacgat agcggtagcg
    13921 cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt gcttaccata
    13981 cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt ttggcggagc
    14041 gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg tggcgaatac
    14101 ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat ttgacgaaga
    14161 agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg ctggtaccca
    14221 gaagccacag caggatgccc actgcaacaa aggtgatcac accggaaacg cgatggagaa
    14281 tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta acaggtcttt
    14341 gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta ttccctgggg
    14401 actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc cttcctccgg
    14461 tctgcttgcg ggtcagacag cgtcctttct ataactgcgc gtcatgcaaa acactgcttc
    14521 cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg tgttccggag
    14581 acctggcggc agtataggct gttcacaaaa tcattacaat taacctacat atagtttgtc
    14641 gggttttatc ctgaacagtg atccaggtca cgataacaac atttatttaa tttttaatca
    14701 tctaatttga caatcactca acaaagttgt tacaaacatt accaggaaaa gcatataatg
    14761 cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag tgtggaagta
    14821 ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt cggattgcta
    14881 agtacttgat tcgccattta ttcgtcatca atggatcctt tacctgcaag cgcccagagc
    14941 tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa acgggtaaag
    15001 ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgcaata aggcgctaag
    15061 gagaccttaa atggctgata caaaagcaaa actcaccctc aacggggaca cagctgttga
    15121 actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta ctctcggttc
    15181 aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgtg aatctaaaat
    15241 tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga tcgatcagct
    15301 ggcgaccgat tctaactacc tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac
    15361 tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga tccacgagca
    15421 gattacccgt ctgttccacg ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg
    15481 tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca atcctcgtca
    15541 tcgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg cgatgtgtta
    15601 caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct atgccggtaa
    15661 cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc cgattctgga
    15721 acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg cctctacctc
    15781 caccgtgcgt accgctggct cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat
    15841 tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga
    15901 agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag ataaaaatga
    15961 ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac
    16021 cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag atgacctgct
    16081 ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa
    16141 actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg gtattccgtc
    16201 ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg cccactggag
    16261 cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata caggatatga
    16321 aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt tgtaaatatt
    16381 ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc taaacacaaa aaattgtaaa
    16441 aataaaatcc actatcggac attcatatat t
//
LOCUS       DAJTMK010000090        16380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTMK010000090 DAJTMK010000000
VERSION     DAJTMK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16380
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.091"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..789)
                     /locus_tag="OO135_002446"
     CDS             complement(<1..789)
                     /locus_tag="OO135_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5398259.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGCLPRYLAQRFLDSGALIEKKVVAQ"
     gene            complement(835..1659)
                     /locus_tag="OO135_002447"
     CDS             complement(835..1659)
                     /locus_tag="OO135_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5398260.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(1742..1996)
                     /gene="yoeB"
                     /locus_tag="OO135_002448"
     CDS             complement(1742..1996)
                     /gene="yoeB"
                     /locus_tag="OO135_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5398261.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(1993..2244)
                     /gene="yefM"
                     /locus_tag="OO135_002449"
     CDS             complement(1993..2244)
                     /gene="yefM"
                     /locus_tag="OO135_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5398262.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            2527..2577
                     /gene="hisL"
                     /locus_tag="OO135_002450"
     CDS             2527..2577
                     /gene="hisL"
                     /locus_tag="OO135_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5398263.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    2553..2676
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            2723..3622
                     /gene="hisG"
                     /locus_tag="OO135_002451"
     CDS             2723..3622
                     /gene="hisG"
                     /locus_tag="OO135_002451"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5398264.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTHRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRVQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            3628..4932
                     /gene="hisD"
                     /locus_tag="OO135_002452"
     CDS             3628..4932
                     /gene="hisD"
                     /locus_tag="OO135_002452"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5398265.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASGRLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGMADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            4929..5999
                     /gene="hisC"
                     /locus_tag="OO135_002453"
     CDS             4929..5999
                     /gene="hisC"
                     /locus_tag="OO135_002453"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5398266.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGSVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGIREESQRVIDALRAEQV"
     gene            5999..7066
                     /gene="hisB"
                     /locus_tag="OO135_002454"
     CDS             5999..7066
                     /gene="hisB"
                     /locus_tag="OO135_002454"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5398267.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMLQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            7066..7656
                     /gene="hisH"
                     /locus_tag="OO135_002455"
     CDS             7066..7656
                     /gene="hisH"
                     /locus_tag="OO135_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5398268.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            7656..8393
                     /gene="hisA"
                     /locus_tag="OO135_002456"
     CDS             7656..8393
                     /gene="hisA"
                     /locus_tag="OO135_002456"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5398269.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            8375..9151
                     /gene="hisF"
                     /locus_tag="OO135_002457"
     CDS             8375..9151
                     /gene="hisF"
                     /locus_tag="OO135_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5398270.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASCDGRVVDKSWVSRVAEVIDIPFCVAGGIMSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDAAVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            9145..9756
                     /locus_tag="OO135_002458"
     CDS             9145..9756
                     /locus_tag="OO135_002458"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5398271.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMSPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKTADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(9853..10830)
                     /gene="wzzB"
                     /locus_tag="OO135_002459"
     CDS             complement(9853..10830)
                     /gene="wzzB"
                     /locus_tag="OO135_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5398272.1"
                     /translation="MRVENNNVSGQNHDPEQINLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRDYNAK"
     gene            complement(10977..12143)
                     /gene="ugd"
                     /locus_tag="OO135_002460"
     CDS             complement(10977..12143)
                     /gene="ugd"
                     /locus_tag="OO135_002460"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5398273.1"
                     /translation="MKITISGTGDVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKINFNATLDKNEAYRDAGYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIIKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(12392..13798)
                     /gene="gndA"
                     /locus_tag="OO135_002461"
     CDS             complement(12392..13798)
                     /gene="gndA"
                     /locus_tag="OO135_002461"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5398274.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLTGPKAQPAGEKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(13880..14794)
                     /locus_tag="OO135_002462"
     CDS             complement(13880..14794)
                     /locus_tag="OO135_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5398275.1"
                     /translation="MNKEVIAVIVTYNRKALLLKVIDAVINQSYPLKKILIIDNNSTD
                     GTEMFISNRLSDVVKYKNTGDNLGGAGGFYRGFIEAEKYGYDYLWLMDDDFMPTVNCL
                     EILISNNPDGIVQPVRYNLDETCAELSPLTYDLSNPFKLNPKGTPLKNYLNTIGNKSA
                     KVDIEAIPFEGPLISRKVVEKIGYPEPKFFIFCDDIEYAIKAKRKGIPIQCNLKAKAY
                     RLLVNNQGNDLLSWKGYFMLRNLFYLHKTYGTNFLVRQKPIVLGLGYALSCVLKCNFS
                     QLPVIWRAFWDSSTLRNTEKFRPEMRSK"
     gene            complement(14787..15347)
                     /locus_tag="OO135_002463"
                     /pseudo
     CDS             complement(14787..15347)
                     /locus_tag="OO135_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            15377..16345
                     /locus_tag="OO135_002464"
     CDS             15377..16345
                     /locus_tag="OO135_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5398276.1"
                     /translation="MKDQITHLPDNADRTMAKQKFKLTNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVP
                     LRCPDYTSVSKRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRVASADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETA
                     MYRVKQLFGGSLTLRDYDSQVAEAMALVRALNKMTKAGMPESVRIA"
BASE COUNT         4182 a         4102 c         4011 g         4085 t
ORIGIN      
        1 ttgggcgacc actttcttct cgattaacgc accactatcg agaaaacgtt gcgccagata
       61 acggggtaaa cagccgcagc cgaggccgct aatttgcagc tccagcttgg ttttaaaatc
      121 gaaaacggta atagcttctt gttcgtcgag aagttgtgag gccgtagaag cgccggcctg
      181 tgcagtgtcc cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc
      241 ttcttccagg gctaacggat gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa
      301 ccgagagaaa ccaaactcac tggaagatgg tggctcatgc atcgcgccaa caataatatc
      361 cgctcgcccc tgagtcaggg catcccatga accaccgagt acaccattga taaacttcag
      421 gcgcgtcacg ctatgatgtt gataaaaagc ctcaatgagt ggcgcaagca gagaaaaagg
      481 gaaggtgtcg tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc
      541 ctgcttttcc agttctcgca cggtatgcag aacttcccgc cctttctcta ataacatttt
      601 tccggtgcgg gtgaatttag cgcggtgtcc gctgcgatca agcaattgaa tattaaggtc
      661 gctttccagc ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggccga
      721 cgccgccgca aaactgcctt ctttttctaa ggcatcgagg atcatcaaca catccagcag
      781 tggtttcata cacgccccct tgcagtgtat gcggcgtcct gctggcgtga tcgtttactc
      841 cagcggcatt accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg
      901 actgccatca ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttc
      961 cagccccaat aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc
     1021 acatatatta tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat
     1081 aacatcttct aaatggacta aattaacacc atgttcacca tcaggggcgg tttttccggc
     1141 aaagaagcgt ccgggatgac gtcccggtcc gaccaggccc gcaagacgca gaatatcgac
     1201 cgaagtaccg ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt
     1261 ggtcaccgga ttacgcgggg tggtttcttt caccgtgcct tgcgcgtcgc catagacaga
     1321 tgtcgagctg gtaaaaataa tgcggggaat acgatgggcc agcgcgctat ccactaactc
     1381 ttgtaccgct tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat
     1441 gaccagcgca tcggcatcca tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg
     1501 ctccatgcga agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg
     1561 tgtggtttta ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat
     1621 gcctaaccac cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct
     1681 tagatctata aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg
     1741 ttcaataatg ataacgacat gctgcaatga gcagtgaatc atcagtaacc gcgtatacca
     1801 gacggtgctc ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt
     1861 ctggtttccc cttaccttca aatggtgttc tgcgggtatc tttgataagt tcattgatct
     1921 ttttaactat tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag
     1981 accagattag tttcactcaa taatgtcctt ttccgttcct ttgcctgatt tcaggctatc
     2041 gattgagtcc atcaatctcc gggcgttagc gggggaacgc agtagataag ccgtctcttc
     2101 cagcgagttg tattcttcga gtgacatcag aacacaagcc tctccattct gacgagtaat
     2161 gaggatcggg gcatgatctt caacggcttt catcattgtt gccgacaaat tctgacgcgc
     2221 ttcgctgtag ctaattgtac gcatgtaaat ctcctctttt gtacagttca ttgtacaatg
     2281 atgggtgtta attcactatt tattaattag tttgtagatc aagacattgc cagcgagacg
     2341 aaaaatcaga ctttgctctt tttggtgctg tcaagttaga ggacagtcct cttagccccc
     2401 tcctttcccc gctcattcat taaacaaatc catcgccata aaatatataa aaaagccctt
     2461 gctttctaac gtgaaagtgg tttaggttaa aagacatcag ttgaataaac attcacagag
     2521 acttttatga cacgcgttca atttaaacac caccatcatc accatcatcc tgactagtct
     2581 ttcaggcgat gtgtgctgga agacattcag atcttccagt ggtgcataaa cgcatgagaa
     2641 agcccccgga agatcacctt ccgggggctt ttttattgcg cggttgttaa cggttcagac
     2701 aggtttaaag aggaataaga aaatgacaga caacactcgt ttacgcatag ctatgcagaa
     2761 atccggccgt ttaagtgatg actcacgcga attactggcg cgctgtggca ttaaaatcaa
     2821 tcttcacacc catcgcctga tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt
     2881 gcgtgacgat gacattcccg gtctggtaat ggacggcgtg gtagaccttg ggattatcgg
     2941 cgaaaacgtg ctggaagaag agctgcttaa ccgccgcgtc cagggtgaag atccgcgcta
     3001 ctttaccttg cgtcgtctgg atttcggcgg ctgccgcctt tcgctggcaa cgccggttga
     3061 tgaagcctgg gacggtccgc tctccttaaa tggtaaacgt atcgccacct cttatcctca
     3121 cctgctcaag cgttatctcg accagaaagg tatctctttt aaatcctgct tactgaacgg
     3181 ctctgtcgaa gttgcgccgc gtgccggact ggcggatgcg atttgcgatc tggtttccac
     3241 cggtgccacg ctggaagcta acggcctgcg cgaagtcgaa gttatctacc gctcgaaagc
     3301 ctgcctgatc cagcgcgatg gcgaaatgga agaatccaaa cagcaactga tcgacaaact
     3361 gctgacccgt attcagggtg tgatccaggc gcgcgaatca aaatacatca tgatgcacgc
     3421 accgaccgaa cgtctggatg aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat
     3481 tctaccgctg gcgggtgacc aacaacgcgt agcgatgcac atggtcagta gcgaaaccct
     3541 gttctgggaa acgatggaaa aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc
     3601 gattgagaag atgatggagt gatcgccatg agctttaaca caatcattga ctggaatagc
     3661 tgtactgcgg agcaacaacg ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc
     3721 attacccgca ctgttaacga tattctcgat aacgtgaaag cacgcggcga tgaggccctg
     3781 cgggaataca gcgcgaagtt tgataaaacc acggttaccg cactgaaggt gtctgctgag
     3841 gaaattgccg ccgccagcgg acgcctgagc gacgagctaa aacaggcgat ggcggtggca
     3901 gtaaagaata ttgaaacctt ccacactgcg caaaaactgc cgccggtaga tgtagaaacg
     3961 cagccaggcg tgcgttgcca gcaggtcacg cgcccggtcg cttcagttgg gttgtatatt
     4021 cctggcggct ccgccccgct cttctcaacg gtattaatgc tggcgactcc ggcgcgtatt
     4081 gcgggctgta aaaaagtggt gctgtgctca ccgccgccga ttgccgatga gatcctttat
     4141 gcggcgcagc tgtgcggtgt gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc
     4201 gcgctggcgt ttggtactga atctgtgccg aaagtggaca aaatcttcgg gccgggtaac
     4261 gcctttgtca ccgaagcaaa acgccaggta agccagcgtc tggacggtgc ggcgatcgat
     4321 atgcccgcag gcccgtcgga agtgctggtg attgctgaca gcggcgctac gccggatttc
     4381 gtggcttctg atttgctttc tcaggctgaa cacggcccgg actcacaggt aattttactg
     4441 acgcccgatg ctgatatggc gcatcaagtt gccgaggccg tcgaacgcca actggcagaa
     4501 ctgccgcgtg ccgaaaccgc ccgccaggca ctgagcgcca gccgcctgat cgtgactaaa
     4561 gatttagcgc agtgcgtaga gatttccaac cagtacggcc cggagcacct gatcattcag
     4621 acccgcaacg cccgcgaact ggtcgatggc atcaccagcg ccggttcggt atttcttggt
     4681 gactggtcac cggaatcggc aggcgactat gcctccggca ctaaccatgt tctaccgact
     4741 tacggttaca ccgccacctg ttccagcctc gggatggcag atttccagaa gcgcatgacc
     4801 gtgcaggaac tctcgaaaga aggcttttcc gcgctggctt cgaccattga aacactggcc
     4861 tccgccgagc gcctgaccgc ccataaaaat gccgttactt tgcgtgttaa cgcccttaag
     4921 gagcaagcat gagcaccgtg actattaccg atttagcgcg tgaaaacgtc cgcaacctga
     4981 cgccgtatca gtcagcgcgt cgtctgggcg gtaacggcga tgtctggctg aacgccaacg
     5041 aataccccac tgccgtggag tttcagctta ctcagcaaac gctcaaccgc tacccggaat
     5101 gccagccgaa agcggtgatt gaaaattacg cgcaatatgc aggcgtaaaa ccggagcagg
     5161 tgctggtcag ccgtggcgcg gacgaaggta ttgaactgct gattcgcgct ttttgcgaac
     5221 cgggtaagga cgccatcctc tactgcccgc caacgtacgg catgtacagc gtcagcgccg
     5281 aaacgattgg cgtcgagtgc cgcacagtgc cgacgctgga caactggcaa ctggacttac
     5341 agggcatttc cgacaagctg gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc
     5401 cgaccgggca actgatcaac ccgcaggatt ttcgcaccct gctggagtta acgcgcggta
     5461 aggcgattgt ggttgccgat gaagcctata tagagttttg tccacaggca tcgctggctg
     5521 gctggctggc ggaatatccg cacctggcta ttttgcgcac actgtcgaaa gcttttgctc
     5581 tggcgggcct tcgttgcgga tttacgctgg caaacgaaga agtcatcaac ctgctgatga
     5641 aagtgatcgc cccctacccg ctctcgacgc cggtcgccga cattgcggcc caggcgttaa
     5701 gcccgcaggg gagcgtcgcc atgcgcgaac gggtggcgca aattattgca gaacgcgaat
     5761 atctgattgc cgcattgaaa gaaatctcct gcgtggagca ggtttttgac tctgaaacca
     5821 actacattct ggcgcgcttt aaagcctcca gtgcagtgtt taaatctttg tgggatcagg
     5881 gcattatctt acgtgatcag aataaacaac cctctttaag cggctgcctg cgaattaccg
     5941 tcggaatccg tgaagaaagc cagcgcgtca ttgacgcctt acgtgcggag caagtttgat
     6001 gagtcagaag tatcttttta tcgatcgcga tggaaccctg attagcgaac cgccgagtga
     6061 ttttcaggtg gaccgttttg acaaactcgc ctttgaaccg ggcgtgatcc ctcagctgct
     6121 gaagctgcaa aaagcgggct acaaactggt gatgatcact aatcaggatg gtctgggaac
     6181 acaaagtttc ccgcaggcgg attttgatgg cccgcacaac ctgatgctgc agatcttcac
     6241 ctcgcaaggc gtgcagtttg atgaagtgct gatttgtccg cacctgcccg ccgatgagtg
     6301 cgactgccgt aagccgaaag taaaactggt ggagcgttat ctggctgagc aagcgatgga
     6361 tcgcgctaac agttatgtga ttggcgatcg cgcgaccgac attcaactgg cggaaaacat
     6421 gggcattaat ggtttacgct acgaccgcga agccctgaac tggccgatga ttggtgagca
     6481 actcactaaa cgagaccgtt acgcccatgt agtgcgcaac accaaagaga cgcagattga
     6541 cgtccaggtg tggctggatc gcgaaggtgg cagcaagatt aacaccggcg ttggcttctt
     6601 tgatcacatg ctggatcaga tcgccaccca cggcggtttc cggatggaaa tcaacgtcaa
     6661 aggcgacctc tatatcgacg atcaccacac cgtcgaagat accggcctgg cgctgggcga
     6721 agcattaaaa attgctcttg gcgacaaacg cggtatttgc cgctttggtt ttgtactgcc
     6781 gatggacgaa tgccttgccc gctgcgcgct ggatatctct ggtcgcccgc acctggaata
     6841 taaagccgag tttacctacc agcgcgtggg cgatctcagc accgaaatga tcgagcactt
     6901 cttccgttcg ctctcataca ccatgggcgt gaccctgcac ctgaaaacca aaggtaaaaa
     6961 cgatcaccac cgtgtagaga gcctgttcaa agcttttggt cgcaccctgc gccaggccat
     7021 ccgcgtggaa ggcgataccc tgccctcgtc gaaaggagtg ctgtaatgaa cgtggtgatc
     7081 cttgatactg gctgcgccaa cctgaactcg gtgaagtctg ccattgcgcg ccacggttat
     7141 gaacccaaag tcagccgtga cccggacgtc gtgttgctgg ccgataaact atttttaccc
     7201 ggcgttggca ctgcacaagc agcgatggat caggtgcgtg agcgcgaact gtttgatctt
     7261 atcaaagcct gtacccaacc ggtgctgggc atctgcttag ggatgcaact gctggggcga
     7321 cgcagcgaag agagcaacgg cgtcgatttg ctgggcatca tcgacgaaga cgtgccgaaa
     7381 atgaccgact ttggtctgcc gctgccgcat atgggctgga atcgcgttta cccgcaggca
     7441 ggcaaccgcc tgtttcaggg gattgaagac ggcgcgtact tttactttgt tcacagctac
     7501 gcaatgccag tcaatccgtg gaccatcgcc cagtgtaatt acggcgaacc gttcaccgcg
     7561 gcggtacaaa aagataactt ctacggcgtg cagttccacc cggagcgttc tggtgccgct
     7621 ggcgctaagt tgctgaaaaa cttcctggag atgtgatgat tattccggca ttagatttaa
     7681 tcgacggcac cgtggtgcgt ctccatcagg gcgattacgg caaacagcgc gattacggta
     7741 acgacccgct gccgcgctta caggattacg ccgcgcaggg tgccgaagtg ttgcatctgg
     7801 tggatctgac cggggcaaaa gatccggcaa aacgtcaaat cccgctgatt aaaaccctgg
     7861 tggcgggcgt taacgtaccg gtgcaggttg gcggcggcgt gcgtagcgaa gatgacgtgg
     7921 cggcgttact ggaagcgggc gttgcgcgcg tggtggtcgg ctccaccgcg gtgaaatcac
     7981 cagaaatggt gaaaggctgg tttgaacgct tcggtgccga tgccttagtg ctggcgctgg
     8041 atgtccgtat tgacgagcaa ggcaacaagc aggtggcggt cagcggctgg caagagaact
     8101 cgggcgtttc actggaacaa ctggtggaaa cctatctgcc cgtcggcctg aaacatgtgc
     8161 tgtgtaccga tatctcgcgc gacggcacgc tggcaggttc taacgtctct ttatatgaag
     8221 aagtgtgcgc cagatatccg caggtggcgt ttcagtcctc cggcggcatt ggcgacatta
     8281 acgatgttgc tgctctacgt ggtactggcg tgcgcggcgt aatagttggt cgtgcattac
     8341 tggaaggtaa attcaccgtg aaggaggcca tcgcatgctg gcaaaacgca taattccgtg
     8401 tctcgacgtt cgtgatggtc aggtggtgaa aggcgtacag tttcgcaacc atgaaatcat
     8461 tggcgatatc gtgccgctgg caaaacgcta cgccgaggaa ggtgcagacg aactggtgtt
     8521 ctacgatatc accgcttcct gcgatggccg tgtggtagat aaaagctggg tatctcgcgt
     8581 ggcggaagtg atcgacattc cgttctgcgt ggcgggtggg attatgtctc tggaagatgc
     8641 cgcgaaaatt ctttcctttg gcgcggataa aatttccatc aactcccctg cgctggcgga
     8701 cccgacgtta attactcgcc tggcggatcg ctttggcgtg cagtgtattg tggtcggtat
     8761 tgatacctgg tacgacgctg aaaccggtaa atatcatgtg aatcaatata ccggcgatga
     8821 aagccgcacc cgcgtcactc agtgggaaac gctcgactgg gtagaggaag tgcaaaaacg
     8881 cggtgccgga gaaatcgtcc tcaacatgat gaatcaggac ggcgtgcgta acggttacga
     8941 cctcgaacag ctgaaaaaag tgcgtgaagt ttgccacgtc ccgctgattg cctccggtgg
     9001 cgcgggcacc atggaacact tcctcgaagc cttccgcgat gccgccgttg acggcgcgct
     9061 ggcagcttcc gtattccaca aacaaataat caatattggt gaattaaaag cgtacctggc
     9121 aacacagggc gtggagatca ggatatgtta acagaacaac aacgtcgcga actggactgg
     9181 gaaaaaaccg acggactgat gccggtgatt gtgcaacacg cggtatctgg cgaagtgcta
     9241 atgctgggct atatgagccc ggaagcctta gacaaaacca tcgaaagcgg caaagtcacc
     9301 tttttctccc gcactaaaca gcgactgtgg accaaaggcg aaacgtcggg caatttcctc
     9361 aatgtggtga gtattgcgcc ggactgcgac aacgacacgt tactggtgct ggcgaatccc
     9421 atcgggccaa cctgccacaa aggcaccagc agctgcttcg gcaacaccgc tcaccagtgg
     9481 ctgttcctgt atcaactgga acaactactc gccgagcgca aaactgccga cccggaaacc
     9541 tcctataccg ccaaactgta tgccagcggc accaaacgca tcgcgcagaa agtgggcgaa
     9601 gaaggcgtgg aaactgcgct ggcagcaacg gtacatgacc gctttgagct gaccaacgag
     9661 gcatctgact tgatgtatca cctgctggtg ttgttgcagg atcaggatct ggatttaacg
     9721 acggtgattg agaacctgcg taaacggcat cagtgagttg cggggtaagc ggatgcgatg
     9781 ttgttgtcgc atccggcaaa aaaaacgggc aagatgtcac caccctgccc gttttcttta
     9841 atgctgcaaa gattatttcg cgttgtaatc gcgtagagca ttacgcccca acacaatccc
     9901 cgcgccaacc atgccgccca gcagcaccgc cagaatcaag gtgattgcct ttttcggact
     9961 atcgcgacga ataggtaacg tcggtttcat cacataacgg taagcatgaa tatcaagatc
    10021 atcaactttt aagctttcga tatccagcag gttttgacga gtctgatagt agtttggtga
    10081 gaacaccaac ggacgggtcg cctcatgctt aatcatcgac tccagcgctt cgctccccaa
    10141 aaggaacaac gtatcttgcg tcacatcttc agtctgctga acctgtggct ttgtcacctg
    10201 cgcctgattc gcatactgca acgcttcctg aatctgacgg atacgcagat ctttctgctc
    10261 ctgcgcgacc acttcctggg ttttcagaga gtcctgcaag tttttcatac gcaacgcgat
    10321 gttgtctttc aggtcttttt ccagttcttc atttacctgg tcgtcaactt gctggatata
    10381 cttcgccagc tgcttctgcg caccttcagg tgtttcacca acataagaaa ctgctaaagg
    10441 taatgactga tttttgacgg taggctcaat ggtcagcttt tctgcttctt cctggttatc
    10501 cagcgtttcc gctaatgctg agaatgcagt gctgtaacgt ccgataagtg aagcctgaat
    10561 gtcggagact ttcggtacag ccgatccgta gatcacattc atcgcgttgg tataaccagc
    10621 aatttgcccc acatcaggtt gagtaacgat agctgtggaa gtccactttt ctttggcaac
    10681 ggccagatac ccgatagcca gggcaatagc aacaatgaca gagatgatga ttgtcatctt
    10741 gccacgccac aactgcacca gtaaatcaat caaattaatc tgttccgggt catggttttg
    10801 cccagaaaca ttattatttt ctactctcat cattacccta acggacgaaa taattttaag
    10861 tcgccatagt ttagcgataa tcgccatgaa tgctatagga tatatgataa aaataatgaa
    10921 ttattacaaa gggaacataa gagaatcagg gcgaaaatcg ccctgataac aggatgttag
    10981 tcgctgccaa agagatcgcg ggtgtatacc ttatctgcca catccctaag ctcttctgcc
    11041 atacggttgg aaataatgac gtcggcttgt tgtttgaagg tggcgagatc tcgttccagg
    11101 cgagagttga agaatgagtc ttctttcatc actggctcgt atatgatcac ttcaacgcct
    11161 ttagccttga tacgcttcat aatcccctga atggaagacg cgcggaagtt atctgaaccg
    11221 ctctttataa tcagacgata aatacccacc acttgcggct tgcgtgacaa aatggcatcg
    11281 gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgaaatcag gttattcggc
    11341 acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata accaccataa
    11401 ccaaacgacg gattgttgta atggttgcca attcgcggat cgagacaaac gccttcgata
    11461 atctggcgag tattcagacc taaactttct gcatagctat ccagttcatt aaagtatgct
    11521 acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc ggttgaatcg
    11581 gtgaacagag tcgggatatt ttgcttaatc gcaccttcct gtaacaacgc agcaaaacgt
    11641 tctgcgcgtt ctgaacgctc accgatgaca atacgtgaag gatggagatt atcgtaaagg
    11701 gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg atatttctta
    11761 tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac cgcataagga
    11821 tttatttcaa ctacgtcttt aattactgat tctacactgg atgtattgaa ataattagtt
    11881 ttaggatcat agtcggttgg agtagcgatg atgacataac cagcatcccg gtaggcttca
    11941 tttttatcta atgtggcatt aaagtttatt ttatctgatt gcaaaaactg ctgaatttcc
    12001 ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg taaaatatct
    12061 aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc tacatcgcca
    12121 gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg tgaatcatga
    12181 tgtttttagc atcctgataa gagctaaaag ttttaacgct acgttgttcg atggtcaacg
    12241 caacgaaaaa caattcagat tttctttata agaatagctg atatttatta agttaatatt
    12301 aagcagtgaa atttagtcta taagataatt ggcaaaaaaa agcccggcac aatatacgcc
    12361 gggcctcaat tttattgtta gttagatcag attaatccaa ccattcggta tggaacacac
    12421 cttctttatc aatgcgctta taagtatgtg caccgaaata gtcacgctgt gcctggatca
    12481 ggttcgcagg cagaacagca gaacggtagc tgtcataata ggcaaccgca gcggcgaagg
    12541 tcggaaccgg gataccgttc tgtactgcgt aagcgacgac atcgcgcagc gcctgctggt
    12601 agtcatcggc aatttgcttg aagtacggag ccagcagcag gttagcgatc tgcggatttt
    12661 cggcataggc atcggtgatt ttctgcagga actgcgcacg gatgatgcaa ccagcacgga
    12721 aaatcttcgc gatttcgccg tagttcagat cccagttgta ctcttcagac gcggcgcgta
    12781 gctgagagaa gccctgagcg taagaaacga ttttgcccag atacagcgca cgacgaactt
    12841 tttcgatgaa ctcagccttt tcgccagctg gctgtgcttt cgggccagtg agaactttag
    12901 acgcggcaac gcgctgatct ttcagagaag agatataacg tgcaaacaca gactcggtaa
    12961 tcagcgacag cggttcgccg agatccagcg cgctctggct agtccattta ccggtacctt
    13021 tgtttgctgc ttcatccaga atcacatcaa ccaggtagtt accgtcttca tcttttttag
    13081 tgaagatgtc tttagtgatg tcgatcaggt agctgctcag ttcaccgtta ttccactcgg
    13141 taaaggtctg cgccagttct tcgttggtga ggttcaggcc acctttaagc agagaatagg
    13201 cttcagcaat cagttgcata tcaccgtatt caataccgtt gtgaaccatc ttcacatagt
    13261 gacctgcacc atcggcacca atataggtaa cgcatggctc cccgtcttca gctactgcgg
    13321 cgattttggt caggatcggt gcaaccagtt cataggcttc tttctggcca ccaggcatga
    13381 tggacggccc tttcagcgcc ccctcttcac cgccggaaac accggtacca atgaagttaa
    13441 agccttctgc agaaagctca cggttacgac gaatggtgtc ctggaagaag gtgttaccac
    13501 catcaatgat gatgtcacct ttatcgaggt atggcttgag agaatcaata gcagcatccg
    13561 tgcctgcacc tgctttcacc attaacagga tgcgacgagg cgtttccaga gattcaacaa
    13621 actctttcac cgtatagtaa ggaaccagtt ttttgcctgg attttcggca atcacttctt
    13681 ccgttttttc acgggaacgg ttgaaaatag agacggtata accacggctt tcgatgttga
    13741 gcgcaaggtt gcgccccatc actgccatac cgacgacgcc gatctgttgc tttgacattg
    13801 tttactcctg tcagggataa atatcgtgtt aagaatttta gtaaaaatcg acattaatta
    13861 ataaaaacat aataataaat tatttacttc tcatttcggg cctgaatttt tcggtatttc
    13921 ttaacgtaga tgaatcccaa aaagcacgcc atataacggg aagttgagaa aaattgcatt
    13981 tcaatacaca cgacaatgca tatcctaatc ccaatacgat aggtttttgt ctaacaagga
    14041 aattggtccc atacgtttta tgcaggtaga aaagatttcg aagcataaaa tatcctttcc
    14101 aggaaagaag atcattacct tgattgttga ccaaaaggcg gtatgccttg gctttaagat
    14161 tgcactggat gggaatcccc tttcttttag ctttaatagc gtattcaatg tcatcacaaa
    14221 aaataaaaaa cttaggctct ggatagccta ttttttcaac aacctttcgt gaaatcaatg
    14281 gaccttcaaa tggaatagct tcaatatcta ccttcgcaga tttattacca attgtattta
    14341 agtaattttt taatggtgta cctttagggt ttaacttaaa tgggttagat aaatcataag
    14401 ttaatggaga aagctctgca caagtttcat ccaagttata tctaacaggt tggacaatac
    14461 catcagggtt atttgatatt aatatttcaa gacaattcac tgtaggcata aaatcatcgt
    14521 ccattaacca aagataatca tagccatatt tttccgcctc tatgaatcct ctataaaacc
    14581 cgccagcccc ccctaaatta tcccccgtat ttttatactt caccacgtca gagagcctat
    14641 ttgaaataaa catctccgta ccatcagtac tattattatc tattatcaat atttttttta
    14701 gtggataact ttggttaata acagcatcta taactttcag caataatgct tttctattat
    14761 aggttacaat aactgcgatt acttctttat tcacctaaac gtacctcttt tcggaatgca
    14821 tttaaagctg atttgataac ttgatgcatg tcataatatt tatattccgc aagacgtcca
    14881 ccaaaaatca cattactttc ctgtttagaa agattataat acattttata gatttgcata
    14941 tttttttcat cattgacagg atagtaatac tcatcgcctt cttcgaattc ttttgggtac
    15001 tctcgagtaa tgagagtatt atgctgttca ccaaattcaa aaaatttatg ttctattata
    15061 cgagtgaatg ggaatttttt gtctatataa tttacaacag cattcccttg aaaattatcg
    15121 acattcagta attcatgctc aaattttaat gatcgataat ccaaagctcc aaatttatag
    15181 caataaaact tatcaatagg ccctgtataa ataattttct ttgcaataga atcccaatat
    15241 ttcttctctt caaaatagtc agtgcatgtt tgaacttcaa taccatttaa taatgcatcg
    15301 attaatttat tgtaaccacc gactggtatt ccttgatact ggtcggattt gttgaataaa
    15361 tcgaactttt gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgtac
    15421 catggcaaag caaaagttca aactcaccaa ctggcccacc tacaataaag ccctcatcaa
    15481 ccgtggctcc ataactttct ggctggatga tgaagctatt caggcctggt atgagtcggc
    15541 aacgccttca tcacggggaa gacctcagcg ctattctgat ctcgccatca ccaccgttct
    15601 cgtcattaaa cgcgtgttca ggttgaccct gcgggctgca cagggtttta ttgattccat
    15661 ttttacactg atgaatgttc cgttgcgctg cccggattac accagtgtca gcaagcgcgc
    15721 aaagtcggtt aatgtcagtt tcaaaacgcc cacccggggt gaaatcgcac acctggtaat
    15781 tgattccacc gggctgaagg tcttcggtga aggcgagtgg aaagtcaaaa agcatggcca
    15841 ggaacgccgc cgtatctggc gtaagctgca tctcgccgtt gacagtaaaa cacatgaaat
    15901 catctgcgct gacctgtcgc tgaacaatgt gacggactca gaagccttcc cgggtcttat
    15961 ccggcagact cacagaaaaa tcagggtagc atcggcagac ggcgcttacg acacccggct
    16021 ctgtcacgat gaactgcggc gtaagaaaat cagcgcgctt atccctcccc gaaaaggtgc
    16081 gggttactgg cccggtgaat atgcagaccg taaccgtgca gtggctaatc agcgaatgac
    16141 cgggagtaat gcgcggtgga aatggacaac agattacaac cgtcgctcga tagcggaaac
    16201 ggcgatgtac agggtaaaac agctgttcgg gggttcactg acgctgcgtg actacgatag
    16261 tcaggttgcg gaggctatgg ccctggtacg agcgctgaac aaaatgacga aagcaggtat
    16321 gcctgaaagc gtgcgtattg cctgaaaaca caacccgcta cgggggagac ttacccgaaa
//
LOCUS       DAJTMK010000091        15909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTMK010000091 DAJTMK010000000
VERSION     DAJTMK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15909
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.092"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..173)
                     /locus_tag="OO135_002465"
     CDS             complement(<1..173)
                     /locus_tag="OO135_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCR5398277.1"
                     /translation="MFDYQVSKHPYFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(206..427)
                     /locus_tag="OO135_002466"
     CDS             complement(206..427)
                     /locus_tag="OO135_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5398278.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            553..1122
                     /locus_tag="OO135_002467"
     CDS             553..1122
                     /locus_tag="OO135_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCR5398279.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFDCKIEDIEIIGKIISLTVKYVR"
     gene            1138..1341
                     /locus_tag="OO135_002468"
     CDS             1138..1341
                     /locus_tag="OO135_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398280.1"
                     /translation="MLGKVFFVVLSCSLSCSLLLSPLTTYAKNYPCSGKKGGVSHCTS
                     DGKFVCNDGTISKSKKICTKNSR"
     gene            1602..2582
                     /locus_tag="OO135_002469"
                     /pseudo
     CDS             1602..2582
                     /locus_tag="OO135_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2687..3223)
                     /gene="rcdA"
                     /locus_tag="OO135_002470"
     CDS             complement(2687..3223)
                     /gene="rcdA"
                     /locus_tag="OO135_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5398281.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            3307..4515
                     /locus_tag="OO135_002471"
     CDS             3307..4515
                     /locus_tag="OO135_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398282.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPVIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGAWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            4515..5330
                     /gene="ybjI"
                     /locus_tag="OO135_002472"
     CDS             4515..5330
                     /gene="ybjI"
                     /locus_tag="OO135_002472"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5398283.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            5416..5700
                     /locus_tag="OO135_002473"
     CDS             5416..5700
                     /locus_tag="OO135_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398284.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPTPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(5741..6970)
                     /gene="mdfA"
                     /locus_tag="OO135_002474"
                     /pseudo
     CDS             complement(5741..6970)
                     /gene="mdfA"
                     /locus_tag="OO135_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            7259..7855
                     /gene="ybjG"
                     /locus_tag="OO135_002475"
     CDS             7259..7855
                     /gene="ybjG"
                     /locus_tag="OO135_002475"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5398285.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7913..8671
                     /gene="deoR"
                     /locus_tag="OO135_002476"
     CDS             7913..8671
                     /gene="deoR"
                     /locus_tag="OO135_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5398286.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(8718..9920)
                     /gene="dacC"
                     /locus_tag="OO135_002477"
                     /pseudo
     CDS             complement(8718..9920)
                     /gene="dacC"
                     /locus_tag="OO135_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            10167..10792
                     /gene="gstB"
                     /locus_tag="OO135_002478"
                     /pseudo
     CDS             10167..10792
                     /gene="gstB"
                     /locus_tag="OO135_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(10789..11904)
                     /gene="yliI"
                     /locus_tag="OO135_002479"
     CDS             complement(10789..11904)
                     /gene="yliI"
                     /locus_tag="OO135_002479"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR5398287.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKISEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(12015..12398)
                     /gene="bssR"
                     /locus_tag="OO135_002480"
     CDS             complement(12015..12398)
                     /gene="bssR"
                     /locus_tag="OO135_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5398288.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            12611..13936
                     /gene="rimO"
                     /locus_tag="OO135_002481"
     CDS             12611..13936
                     /gene="rimO"
                     /locus_tag="OO135_002481"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5398289.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVNPGDILRVKVEHADEYDLWGSRV"
     gene            complement(13983..15311)
                     /locus_tag="OO135_002482"
     CDS             complement(13983..15311)
                     /locus_tag="OO135_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5398290.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIHVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAVINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(15319..>15909)
                     /locus_tag="OO135_002483"
     CDS             complement(15319..>15909)
                     /locus_tag="OO135_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5398291.1"
                     /translation="IGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLN
                     EPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFG
                     TGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESED
                     VARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         4102 a         3932 c         3768 g         4107 t
ORIGIN      
        1 agccagattt ctggtgcggt taataaatga ggctgagctg ggttcagctt gttccgcaga
       61 atctgcacat tcatgcctgc acgttctgcc agttgcacca gattgtggcg cagtgcaaat
      121 gcacgacagg cttcatcaaa atatggatgt ttggaaactt ggtaatcaaa catggtcaat
      181 gcctctgatg tatttcagaa tcgaactaat taaggtttag attgcattct gaaagcgcat
      241 caacggtcat tgctgctatg ttgatcatca ctttttcgcg ttttttatct ttgcgcagac
      301 ggtgacggat aaggcgtcca tcagccaaca tgtcattgat ggtatcgatg gatagccctg
      361 tcagctcgct atagcgttca atagtcacat gaggcgtggt aagagtgatt gaaatgttag
      421 gtctcatgat gcaacattcc tcgtttaatg atgattaatc aggacgaata cggatcgttt
      481 gtattttgtg aacaccataa gcatacgatc gcacagtgaa atcgccaaga taaaagttca
      541 cttggagtga ccatgaattt ggagaaaggc ggacgaggcg ccatagagcg catggtagaa
      601 gcttatggat tcaagactcg acaggcgttg tgcgatcatt taggaatctc taaaagtaca
      661 ctcgccacac gctacatgcg tgactcattc ccagcagaat gggtaatcca gtgcgccctt
      721 gaaacgggca cctcgcttaa ttggctcaca accggacatg gttcaaagca aacttcaggt
      781 aatacaaata ctatggaagt tgctaaatat gtattatctg atggtgcctt gcgtgaagac
      841 ggtttttata tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc
      901 acagataaca attctgaatt tatttgtgat aaagaatttg atgacatacg tgatggtaaa
      961 tgggtaataa gtattgatgg cgaagtaacg atccgtgaca ttactcgttt acccggtgga
     1021 agaatcttcg tcgagggtgg aaacagagcc ttcgactgta agatagaaga cattgaaata
     1081 attggtaaaa ttataagttt aacagttaaa tatgttagat aataccggga ggaaactatg
     1141 cttggtaaag tattttttgt ggttttatca tgctctttat catgctcttt gttattaagc
     1201 ccactaacta cctatgctaa aaattatcct tgttctggga aaaagggagg tgtctctcac
     1261 tgtacctccg atggaaaatt tgtttgcaat gatggaacta ttagtaaatc caaaaaaatc
     1321 tgtactaaaa actcacgata acttttgctt ttatatctgc gtctaaaata aaaatgagcc
     1381 gcaggttaac cgcaaaagtt acatgctcac atagcaaaaa gaatagccaa cttcattatg
     1441 gcttcagtga gatgtatggt cgtaggattt catacattga cactggttat acatacagta
     1501 aaaatactct ctactggagg gcatttttta tggcagtacg aaaactcacc actgggaaat
     1561 ggctttgcga atgttaccct gccggacgaa gtgggcgtcg tgtgcgtaaa caattcgcca
     1621 ccaaaggcga agcactggct tttgagcgcc acacgatgga agaaaccgaa gcaaagccct
     1681 ggctgggtga atcagtggat cgtcgaacac tgaaagacgt ggttgagcta tggttcaaac
     1741 tacatggtaa atctctgaca gctgggcagc atgtctatga caaattgctg ttgatggttg
     1801 acgctctggg caatcctctt gcaaccgatc tcacctctaa aatgtttgcc cactatcgag
     1861 ataaacgcct gacaggcgag atctacttca gcgagaaatg gaagaaagga gcaagcccgg
     1921 tcaccattaa cctggagcaa agctatctaa gtagtgtttt tagcgaacta tcccgtctgg
     1981 gcgaatggtc gtatccgaac ccactggaga acatgcgaaa attcaccatc gcagaaaaag
     2041 agatggcatg gcttacccat gagcagattg ttgaattgct ggctgattgc aaacgtcagg
     2101 acccaattct ggcactggta gttaagatat gcttaagcac aggcgcacgc tagcgtgaag
     2161 ccgtaaatct tacccgctca caggtgacca aataccgaat tacctttgtc agaacgaagg
     2221 ggaagaaaaa cagaagcatc cctatcagta aagagcttta cgaagagatc atggcgcttg
     2281 atgggttcaa tttcttcaca gactgctatt ttcaattttt atccgtgatg gaaaaaacgt
     2341 ctatcgtgct ccctcgcggt caactcacac acgttctgcg ccatacgttt gcggcgcact
     2401 tcatgatgtc gggtggaaac attctggcct tacaaaaaat tctcggacac cacgatataa
     2461 aaatgactat gcgttacgca catctggcac tggatcatct ggaaacggcg ctccgtttca
     2521 accctctggc aacgctgcca agtggcgaca aagtggcggc agcggttggc attaccccgt
     2581 aataaccacc actgaccacc aacctaactt attgttttta atctaactta ttgttttcat
     2641 taacccgttt acataaatgg tttttttgtt atctaaaatt tatctactac cctgcaaccc
     2701 tctcaaccat cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg acaaaatgca
     2761 gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt tccgggttca aaccattgtt
     2821 cgagcgtttg ctgactgcgc tgcatccagt tttgcattac cgtttttaat agcggttttc
     2881 ggctagccag cgcgtagagc tggtacatca gctccatatt atccggcgtt gcaacctgtg
     2941 agctgtagat catatcggtg atagcctggc atgcgccttg agcatcacta acatcgctaa
     3001 aaaatgcctg atattgacgg gacataatct cagtaaaact gctgaacgcc tccagcaaca
     3061 actcatcaat tcctgaaaaa tagtaggtca tcgatcccaa cggtaccccg gcaagggtag
     3121 caattttgcg gtgcgtaaca gcatgtattc cgtaaagttt caccgcctcc agcgtggcct
     3181 ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt cctctgtctg
     3241 caattgtgta caaatgtaca caaccttgct aatgttgtct tccctcttct tattttgacg
     3301 tgatctatga ccgtaaattc ttcacgtaat gcattgaaac gccgaacctg ggcgctgttt
     3361 atgttcttct ttttgcctgg cctgttaatg gcgtcctggg caacccgtac gcctgttatc
     3421 cgcgatattc tctctgtctc gatcgctgaa atgggcggtg ttctctttgg tctgtcgatc
     3481 ggttcgatga gcggtattct ctgctcggcg tggttagtga aacgctttgg gacacgtaat
     3541 gtcatcctgg tcacgatgtc ctgcgcattg atcgggatga tgatattaag tctggcactc
     3601 tggctgacat cgcccctgct ctttgccgtt ggtctcggcg tctttggggc aagttttggt
     3661 tctgcggaag tggcgataaa cgttgaaggt gccgccgttg agcgagaaat gaataaaacg
     3721 gttttgccga tgatgcacgg tttttatagc ctgggcacgc tggcaggcgc tggtgtcggg
     3781 atggcactga cggcctttgg cgttccggca acggtgcaca ttttattggc ggcgctggta
     3841 ggtatcgcgc ctatttatat cgccattcag gcaatccctg acggtacggg caaaaatgct
     3901 gccgatggca cccagcatgg cgaaaaaggc attccttttt atcgcgatat ccagttgctg
     3961 ttgattggtg ttgtagtgct ggcgatggcc tttgccgaag gttctgccaa cgactggtta
     4021 cccttattaa tggttgacgg tcacggtttt agccctactt ccggctcgct gatttatgcc
     4081 ggttttaccc tggggatgac cgttggacgc ttcactggcg cttggttcat cgaccgttac
     4141 agtcgcgttg ccgtggttcg ggccagtgca ctaatggggg cgttgggtat tgggctgatt
     4201 atttttgtcg atagcgcctg ggtcgctggg gtgtctgttg tactctgggg actgggtgcc
     4261 tcgctgggct tcccgctgac catttctgcc gccagcgata ccggccccga tgcaccaacc
     4321 cgcgtcagtg tggtagcaac gaccggttat ctggctttcc tcgtcgggcc gccgctgctg
     4381 ggctatctcg gcgaacatta tggattacgt agtgcaatgc tggttgtact ggcgctggtt
     4441 attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac gcagacggcg
     4501 atggagaata gttgatgagc attaaattaa ttgcggtaga catggatggt actttcttaa
     4561 gcgatcaaaa aacctataac cgtgagcggt ttatggctca gtatcagcaa atgaaagcac
     4621 aaggcattcg ctttgtggtc gccagcggga atcaatatta tcagttgatc tctttcttcc
     4681 ctgaaattgc taatgaaatt gcctttgtgg ctgaaaacgg cggctgggta gtgagtgaag
     4741 gcaaagatgt ttttaatggc gagctgtcga aggatgcctt tactactgtc gtggaacatt
     4801 tgctgacgcg cccggaagtg gaaattattg cctgcggaaa aaatagcgcc tatacgctca
     4861 aaaaatatga cgatgccatg aaaacggtgg cggaaatgta ttatcaccgt ctggaatacg
     4921 tcgataactt tgacaactta gaagatattt tctttaagtt tggcctgaat ctttccgatg
     4981 aactgattcc acaagtacaa aaagcattac atgaggccat cggcgatatt atggtgccag
     5041 tccacaccgg caacggcagc atcgatctga ttatccccgg cgtacataaa gccaatggcc
     5101 ttcgccaact gcagaaatta tggggaatag acgacagcga agtggtggtc tttggcgatg
     5161 gcggtaacga tattgagatg ctgcgtcagg caggctttag ttttgcaatg gaaaatgccg
     5221 gcagcgcggt cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt gaaggcgtac
     5281 tggatgtgat cgataaagtg cttaaacacg aagcgccatt tgaccaataa aaacggcagc
     5341 acgcccggca acacaatacc tgaatgaagg ctgcataatt cgcggaaatt aacccgctgc
     5401 attatttcag gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc
     5461 tttactggtg tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg atacaaagtg
     5521 gttgtagttc ctgattcagt cagcgttcca caagcggtca gcgtcgcgac tgtgccgcaa
     5581 accgtctatg tggctcctac gccagctccg gcttaccgcc cgcatcctta tgctcgccat
     5641 cttgccagcg taggtgaagg gatggttatc gaacaccaga ttgacgacca tcaccattaa
     5701 ttcgagaatg cctaatcgca caaagcagtc aggcattttt ttacccttcg tgagaatttc
     5761 ccatctgttt atcttttaaa aagataacca tcagcgacaa ccacaaaatt ccgttgacaa
     5821 ggttgaagag attaaactgt ccgttgcccc cgttcagcca ggcatgtttg ctgatttcaa
     5881 taccaacggt aaagatcagc atttgcagca ttcccatcgc cgcagaaacc gtacctttac
     5941 tcatatcgct ggcaaacagg gttaatcgca ccagtcccgc attcgccaga ccaataccga
     6001 aagcataaat acttaacccg gcagtcatcc ataaatacgc gtgcgatgag ataaccgttg
     6061 ccgcagcagc gaccaacaga ccaatcataa tcggccagcc gcccataatt atcagcgaac
     6121 gtacggtgcg gcgcgaggtc agacgcgcta acagcaagtt acccgcaatt aacgccccga
     6181 aaataggcac ttgcagcaag ccatattcat agctgctcaa ctgctcgccg gtaatgatga
     6241 taatcggcga ctgggcgatc cacgccagca atggcaggct aacgaatccc agcgccagcg
     6301 cccccgccac aaagcggccg ttcttcagca ccagcttata gtcacgaccg agttctttca
     6361 gtgacagttt ctcgcctata cgcgtggcgg tttcaggcat agctcgttgc agaccgaaaa
     6421 aggagatcgc tgccaatgcg gcaaacaaaa caaacatccc ctcccagggc agcacatgga
     6481 tccacgccgc gcccaccagc ggaccaagta gcggagcaat cagcgccacg ttcgccatca
     6541 gcgcggtgat cttgatacaa accgcctctt cgaaagattc ctgaattgcg gcgtatccca
     6601 cagcgccaat gaaacagagg cttatgccct gcaagaagcg caacagagtg aattgttcaa
     6661 tgttttgcgc cagtaatatt gccagacagg tgatgataaa ccacaccccc ccgccagcat
     6721 caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta aaaacatccc
     6781 gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct gatattgttc
     6841 caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa aattcgtaaa
     6901 gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg gaagctaatt
     6961 tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa aagcgcagct
     7021 aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac attattgcga
     7081 gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc acccaccgta
     7141 aacaggccgc ttgagggaaa taagacgatg ccgctttacc cacccagttt aacctgcact
     7201 ttattctcaa cgacttgcct gtattggctc ccttttaatc actttgcgtc gggaagttat
     7261 gctggaaaat ttgaatctct ctctattctc tcttattaac gcgacgccag actcggctcc
     7321 gtggatgatc tcgttggcga tttttattgc taaagatttg attaccgtgg tgccgctgct
     7381 ggccgcggtg ctttggttgt gggggcttac agcacaacgg caactggtga taaaaatcgc
     7441 tatcgcgctg gcggtcagcc tgtttgtgtc ctggacgatg ggacatcttt ttccgcacga
     7501 ccgacccttt gtcgaaaata tcggctataa cttcctgcat catgcggcgg atgactcatt
     7561 cccaagcgac cacggtacgg tgattttcac ctttgcactg gcatttttat gctggcatcg
     7621 cctgtggtcc ggctcacttt taatggtgct ggccgtcgtc attgcctggt cgcgcgttta
     7681 tcttggcgtc cactggccgc tggatatgct cggtggattg ctggcaggta tgattggctg
     7741 ccttagtgcc cagattatct ggcaagcgat ggggcataaa ctctatcaac gtctgcaatc
     7801 gtggtatcgc ttctgttttg cattaccgat ccgcaaaggc tgggtgcgtg actgatctct
     7861 gctaaaaagt gtagtattga gcggctcgct tcaataacta ttcagaggga ttatggaaac
     7921 acgtcgcgaa gagcgtatcg ggcagctgct gcaagaatta aaacgcagcg ataagttaca
     7981 tcttaaagac gccgccgccc tgcttggggt ttcggagatg acgattcgtc gcgatctgaa
     8041 caaccacagt gcgcccgtcg ttttgctcgg cggctatatt gttctggaac cgcatagtgc
     8101 cagccattac ctgttaagcg atcaaaaatc ccgcctggtg gaagaaaaac gccgggcggc
     8161 aaaactggct gcgacactgg tagaacccga tcagaccctc ttttttgact gtggcaccac
     8221 cacgccgtgg attattgaag cgattgataa tgaaatccct tttaccgccg tttgttattc
     8281 gctaaatacc tttctggcgc tgaaagagaa accccattgc cgcgcatttc tgtgcggtgg
     8341 tgaatttcac gccagcaacg ccattttcaa acccatcgat tttcagcaaa cgttgaataa
     8401 tttttgcccg gatatcgctt tttattccgc ggcgggcgtg catgtcagta aaggcgctac
     8461 ctgttttaat cttgaagagt tgccggtaaa acactgggcc atgtcgatgg cgcaaaagca
     8521 tgtgctggtt gtcgaccaca gtaaatttgg caaggtccgt ccggcgcgca tgggtgacct
     8581 gaaacgcttt gatattgtgg tgagtgattg ttgcccggaa gatgagtatg tgaagtacgc
     8641 gcagacgcac cgtattaagt tgatgtatta atgacgtata accggatgac gtttcgcgcc
     8701 atccggttat cagaagatta agagaaccag ctgccgaacc actgatggaa tttcatcatc
     8761 acgaaatccc acatccgacc aaagaatccg ccctcttcca cattttccat cacgatcagc
     8821 ggacgctgct caatggattt accgttaagc tggaaatcaa tggtcccgac aacctgacct
     8881 tttttcagcg gtgcggtaag ctgcggttcc gttaacgtgt agctcgcctt caggtttttc
     8941 agctgcccac gcggtatggt cactgagccc gcttcgcctg ccccgagatt cacttcgctc
     9001 ttatcaccaa accagacgcg ctgagtcaca aaggtggtat caggtttaat tggcgtcacg
     9061 gtttcaaaga agcggaaacc ctaggtcaat aatttctcag actcattaaa acggatacgg
     9121 tcagttttcg cccccaacac tacggagatt aaacgcatat cgccctgggt agccgaagca
     9181 accagattat atcccgcgcc tgccgtggtt cctgtcttca tgccatcaac attcagattg
     9241 ctgctccaca gcagacggtt acggttaggc tgacgaattt tgttgaaggt gaactctttc
     9301 tctttatgaa tggcgtactc ttccggcaca tcgtggatca atgctttacc cagcaatgcc
     9361 atatcgcgcg cggtactgaa ctgccccggc gcatccaggc cgtgcaccgt ctggaaggta
     9421 gtgttggtca gacccagttt tttggcataa ccattcatca gaccaataaa tgactcctgg
     9481 ctcccggcaa cgtaatcggc cagcgcaata caggcgtcat taccggactg gataatcaca
     9541 cctttgttca agtctgccac cgaaacctga tcgcccggtt tgaggaacat taccgatgaa
     9601 ccacgcagtg ccggatttcc cgtcgcccag gcatctttac cgaccgtcac catatcggtg
     9661 agtttaatct tatcggcctt aagcgcctgc ccaaccacat agctggtcat gattttagtc
     9721 aggctcgcgg gatccagttt ctcatccgca ttgccttctg ccagcacttt accgctggcg
     9781 taatccatta aaatccatgc acgcgcatcc acgctcggcg cttcaacggt ttgttcggcc
     9841 gcgaatgccg ttggggcaaa aaggaataaa aatgcagaac ccgctgcaag accacgaagg
     9901 agagaggagt attgcgtcat aaatgccacc cgagtatcca ttcaaaaaaa atacgttata
     9961 tcgccgtatg ataagttcgg tgattaataa cgcacacaca aacttaaaga aaccgtaagt
    10021 tggtaaagtt tttaaagttt atgcaatatc ctgccgcttc gctgcttctg gtgtcatttt
    10081 tttgccagct gttgcttaaa agttaacttt acttcaccat cggagtcctg ttcagcctgt
    10141 ggctgcctgt cggaaaaggg gtgagtatga ttacgctgtg gggtcggaat aattcaacca
    10201 acgtaaaaaa gtattgctga cgctcgaaga actggaacta ccttatgagc aaattctcgc
    10261 gggccgtgag tttgggataa atcacgatgc tgattttctg gcgatgaacc ctaacgggct
    10321 ggtgccgttg ttgcgtaacg acgaaagtga tctcattctt tgggaatcaa acgccattgt
    10381 ccgctatctg gcggcacagt acgggcaaaa acgcctgtgg atcgactcac cggcacgtcg
    10441 tgcggaagcg gaaaaatgga tggactgggc aaaccagacg ctcagtaatg cccatcgagg
    10501 gatcctgatg ggattagtca gaacaccacc ggaagagcgc gatcaggccg ccattgatgc
    10561 cagttgcaaa gagtgcgacg ccctgtttgc cctgctcgat gcggaactgg caaaagtaaa
    10621 atggttctct ggcgacgagt ttggtgtggg cgatatcgct atcgcaccgt tcatctacaa
    10681 tttgtttaac gttggcctga cctggacacc gcgtccaaat ctgcaacgct ggtatcagca
    10741 actcactgaa cgccccgcag tgcgcaaagt ggtgatgatt cccgttagct aattgcgtgg
    10801 gctaacttta agtaattccc cactggactc gtcggtgaga acgtataaat aaccgtcggg
    10861 tccagtgcga acatcacgaa ttcgctgccc tctgtccgtt aaaatacggc catcttctgt
    10921 cactttgtcg ccgttgacgc tcatcacaat gacatcttta tctttcagcg cgccaataaa
    10981 taatttttgc tgccactggg ggaatttatc gctgttatag aaggccatgc cgctcacagc
    11041 gggcgaatct ttccagtaaa aaacaggttg ctcggtcccg gcgacgatct cccctttcgc
    11101 ttccgatatc ttaaagcctg aatagttgat tccccaggtt gccagcggcc agccgtagtt
    11161 tttgcctttt tgcgggatat taatttcatc accaccgcgc gggccatgtt cattcagcca
    11221 cagtgcatta ctccacggat tcatcgccat tccttgcgga ttacgaatgc cataagacca
    11281 gatctcggcg cgcgcaccgg attcctttat aaaaggatta tcatccggga tttcgccctg
    11341 atcggtcaga cgcaccagtt tgccctgtaa tttatccaga tcctgcgccg tcgggcgctg
    11401 attgttttcg cccagagcaa taaaaagata acctttaccg tcgaatacca gccgcccgcc
    11461 aaaatggttg ccggtagaca gttttggcat ctggcggaag acggtgcgga agtcggtcac
    11521 ttttgagaga tcatcactta agcggccata acccacagca gttccggctt tgccatcatc
    11581 gccaacttcg gaataactta accagatgcg gcgagactga gcaaaatcag gcgctaaaac
    11641 cacgtccagc aggccgccct gcccgtgcgc ccaaacgtcc ggaactccgg aaagcggcgc
    11701 agataatcct tttcctgctt gccagtgacg caactcgccg ccgcgcagag tgattaacat
    11761 accgtgatta tcgggtaaaa aggccagtgc ccagggatgg tcgagtttgt cttgcagtac
    11821 ttcgacattt accgttgcag gagccgccca gagagcggaa gaaagacaaa taaggggcac
    11881 aaggaaaaag gattgtcgat gcataacgcc tccggattga ctctggctta aagcgtagtc
    11941 agtggaggag ataacaaatt catttttaca aaaacttaaa catgaagggg gagacgcttt
    12001 ctccccctta gttttcaggc cttctcaagc atggcgtgct tctgcaggct ctggatactc
    12061 agcgttaagc tcatcagaca attttcaagc ttatcggcgt tgacggtaat aacagtcggg
    12121 caatcatggt gcccactcat caaacatact gcggctgtcg ctaatgcttc ttcagcatga
    12181 tgaagagcac tccactcttc ctgatccaga tgaagattca accgcagcga tttatcgtgc
    12241 agttcgcgat tcagtttaaa aaagttatct cgtagatgat tgctttcgct gacggacatg
    12301 tatccttttg ccttcctcag ttgcacgtat gcagcgaggt caacgcgtgc gtcgatcaac
    12361 cggttcagca gatcgattcg ctgtctgtca acaaacatac ttcgatcctc ctctttccag
    12421 ccttgttgca attaaatagt atggctattt tttgagcaaa atgctgtcca ggatcaattc
    12481 ttcacgccga attaacaaaa tttacatttc cttcgcctcc gccagcgaat gagattgcga
    12541 tacaaatttt atccagaact ctgtagaatc cctgccctga cctttcctgt aactgaatat
    12601 tttttaagct atgagcaaag taactcccca gccgaaaatc ggctttgttt cccttggctg
    12661 tccgaaaaac cttgtcgatt cagagcgtat tctcaccgaa ctccgcactg aaggttatga
    12721 cgtggtaccg agctatgacg atgcggacat ggtgatcgtc aacacctgcg gctttattga
    12781 cagcgcggta caagaatcac tggaagccat tggtgaagcg ttgaatgaaa acggcaaggt
    12841 aattgtgacc ggttgtctgg gggcaaaaga agatcagatc cgcgaagtcc acccgaaagt
    12901 gctggaaatc accgggcctc atagctatga gcaggttctg gagcacgttc atcactacgt
    12961 gccaaaaccg aaacacaacc cattcctgag cctggtgcca gaacaaggtg tgaaactgac
    13021 gccgcgtcat tatgcctatc tgaaaatttc ggaaggctgt aatcaccgct gcaccttctg
    13081 cattattccg tctatgcgcg gcgacctggt gagccgtccg attggcgaag tgttaagtga
    13141 agcgaaacgt ctggtagatg cgggcgttaa agagattctg gtgatctcgc aggatacttc
    13201 cgcctatggc gttgatgtta aacatcgtac tggcttccac aacggcgagc cggtaaaaac
    13261 cagcatggtc agcctgtgcg aacagttatc gaaactgggg atctggacac gtctgcacta
    13321 cgtttaccct tatccgcatg tggacgacgt catcccactg atggcagaag gcaaaatcct
    13381 gccgtatctg gacattccgt tgcagcacgc cagcccgcgc attctcaaac tgatgaagcg
    13441 tccgggttct gtagatcgcc aactggcgcg catcaaacag tggcgcgaaa tctgcccgga
    13501 actgacccta cgctcaacct ttattgtcgg cttccctggc gagacagaag aagatttcca
    13561 gatgctactc gacttcctga aagaagcgcg tctggatcgc gttggctgct ttaaatacag
    13621 cccggttgaa ggtgcagacg ccaatgccct gcctgaccag gttccggaag aagttaaaga
    13681 agaacgctgg aaccgtttca tgcagctgca gcagcagatt tccgccgagc gcctgcaaga
    13741 gaaagtgggc cgtgaaattc tggtaattat cgacgaagtg gacgaagaag gcgcgattgg
    13801 tcgcagcatg gcagatgcac cggaaatcga cggcgcggtt tatctcaatg gtgaaaccaa
    13861 cgttaatccg ggtgatatcc tgcgtgtgaa agtcgagcac gccgatgagt acgatttgtg
    13921 gggtagccgg gtttaattaa ttcaggccgg gcaaaatcat ttgtccggcc tgcgaataat
    13981 gattactgcc agctaatctg agccctgccg tttttcttac tcttatacag ccgctcatcc
    14041 gccagcattt tcaagcgttc gacggtggta cattcatttg aatgcgctac accgatactg
    14101 gtacttaaat taatgcttcc cacctggaaa tgaacgattt cttttagaat cgatagcaac
    14161 tcttccagcg ttccacccgt ataaatcacc gcaaattcgt caccgccgaa gcggtagatt
    14221 ttacctttat ctaccgggat ctgtgattta aggattttca ccactttcat caacacttca
    14281 tcgcccagca aatgaccgaa ggtgtcgtta atgtttttga atttatcgac gtcaaacagg
    14341 cacaaataac cgcctttttc cggcagcgct tttaaatctt cgtcaaagga ttttctgccc
    14401 attgctttgg tcaggtcgtc caggaacgcc gagttataca tactgtagag ttttttgaca
    14461 tcggcagccg aaaagaaaag cccgccatcc agtacgcgtt caaaatggtt attaatgacg
    14521 gcttctttct ccacaatacc acgattaatc actctgcgta ccagatagag gcaactggca
    14581 cccgatatca cagccaccaa tgcggcaaag aaaataatca atgtacagac ccgcaaataa
    14641 acatcttttt tatacgcaaa tacgtacaat ccggtcggca aataggcgaa gtgctccatc
    14701 gcttccgaaa cggtgaagat ggcgttttta gcattatatt catcaccggt caccggttta
    14761 ccgttcttaa atagagagaa catcaggttc tcgctgtcca tcgccagcga ttttgacagt
    14821 aaagaaaaac gctccagacc aagcaaaaat atcagactac gattgtgagt taccggatag
    14881 aggtaataga cgaaggtttt accgtcaaaa gagataaacc ctgtattttt gacatgtcga
    14941 tacttcacaa cctgcataat atctgcacaa taatttttct cgaactcctt tacatgaata
    15001 cattcgtcac tggaaatcag ataaggaccg acatcgtaca aataagaggg ggaaaccgag
    15061 tcggcgtaag ctgtgcgcaa accaaccaac agctgttcct ccgtgggatt tagcgtgttg
    15121 ataagatcaa ttgccacgct acgccgatat tcagcaatat ttgccaccgc aaaagcagta
    15181 tcaataacgg actgtttcag gctatacacc cgttccttta ccatttgagt gtagatcccg
    15241 caggcaactg ctgaaatcat gataaaaatc agcagactga ctgtaagtac gaacttattg
    15301 attctggaca tacgtaaact actcttttac taattttcca cttttatccc aggcggagaa
    15361 tggcataggt ttctggtaca gatacccctg agcctgaaca catcctaatt taattaattc
    15421 gcgcgcaacg tcttcgctct ctacgccttc cgcaatcacc gggatccggt tatagcgtgc
    15481 cagatcgaaa ataatgcgca caatttttcc cgcagtaccg aaaatatcgc cggtaaattg
    15541 cgagtccacc tttatgctgt ctggattaat ttcataaaaa cgtttcaggt ttgacaaacc
    15601 ggtgccaaag tcatcaatcg atatccgtac cccttgttta cgcagttgtt caatattgag
    15661 ataaaaatgt tcattaattc gctcgatcac ggtttcagtg atctcaacac agaggctgtt
    15721 cgccttcagg ccattttctt tcatgatggt ggtaaaacgc tcatgaaagt gcggttcgtt
    15781 taactgccag ggcgaaacat taatatgcag taagaaatca tccttcagcg acgcacgctg
    15841 actaaaatgt ctgaattcac ccattgccgt tttaataatc tgataaccca gctcattgat
    15901 aaacccgat
//
LOCUS       DAJTMK010000092        15349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTMK010000092 DAJTMK010000000
VERSION     DAJTMK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15349
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.093"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(255..1367)
                     /gene="ydiK"
                     /locus_tag="OO135_002484"
     CDS             complement(255..1367)
                     /gene="ydiK"
                     /locus_tag="OO135_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5398292.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1756..4812
                     /locus_tag="OO135_002485"
     CDS             1756..4812
                     /locus_tag="OO135_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5398293.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4809..5219
                     /gene="menI"
                     /locus_tag="OO135_002486"
     CDS             4809..5219
                     /gene="menI"
                     /locus_tag="OO135_002486"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5398294.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRYQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5319..5507
                     /locus_tag="OO135_002487"
     CDS             5319..5507
                     /locus_tag="OO135_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5398295.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFTDLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6054..6422
                     /gene="sufA"
                     /locus_tag="OO135_002488"
     CDS             6054..6422
                     /gene="sufA"
                     /locus_tag="OO135_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5398296.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6431..7918
                     /gene="sufB"
                     /locus_tag="OO135_002489"
     CDS             6431..7918
                     /gene="sufB"
                     /locus_tag="OO135_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5398297.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRVNAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7928..8674
                     /gene="sufC"
                     /locus_tag="OO135_002490"
     CDS             7928..8674
                     /gene="sufC"
                     /locus_tag="OO135_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5398298.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8649..9920
                     /gene="sufD"
                     /locus_tag="OO135_002491"
     CDS             8649..9920
                     /gene="sufD"
                     /locus_tag="OO135_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5398299.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDAFALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRNNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVLARIGQRLPGGAR"
     gene            9917..11137
                     /gene="sufS"
                     /locus_tag="OO135_002492"
     CDS             9917..11137
                     /gene="sufS"
                     /locus_tag="OO135_002492"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5398300.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAVYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11150..11566
                     /gene="sufE"
                     /locus_tag="OO135_002493"
     CDS             11150..11566
                     /gene="sufE"
                     /locus_tag="OO135_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5398301.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11715..12719
                     /gene="ldtE"
                     /locus_tag="OO135_002494"
     CDS             11715..12719
                     /gene="ldtE"
                     /locus_tag="OO135_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5398302.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDCVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(12783..13019)
                     /gene="lpp"
                     /locus_tag="OO135_002495"
     CDS             complement(12783..13019)
                     /gene="lpp"
                     /locus_tag="OO135_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5398303.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13330..14742)
                     /gene="pykF"
                     /locus_tag="OO135_002496"
     CDS             complement(13330..14742)
                     /gene="pykF"
                     /locus_tag="OO135_002496"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5398304.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15300..>15349
                     /locus_tag="OO135_002497"
     CDS             15300..>15349
                     /locus_tag="OO135_002497"
                     /inference="COORDINATES: protein motif:HMM:NF022418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2767 family protein"
                     /protein_id="HCR5398305.1"
                     /translation="MGNRTKEDELYREMCRV"
BASE COUNT         3867 a         3714 c         4060 g         3708 t
ORIGIN      
        1 acacagcaat tcgttgtttc actcagggga tttccatgct tataaatcaa tatgttgatt
       61 tataaccgtg agctaatagt aggcatacgg actttttgcg tcgatcagat tcgtctcatt
      121 agtttaaaaa ctgtaaagaa tttgcgacag gtactaccag ctgaatggcg gtaagcagga
      181 aaatatgggg aagagcaagc aaatatcaat tagcgggggt taacaatcag gcgacagagc
      241 cgcctgatga gaaattactt attcggtttc tcgatttcgc caagttcttc gagaatttct
      301 tccggttggt ccgtcggcgg cgggacttct tccacccacg cggcaaacag acgccaggaa
      361 acggctaaca gaaccggacc aataaacaga ccgatcatcc cgaaagcaat caaaccacca
      421 ataacaccag agaggatcag gatcagcggt aaatcggcac ccatgcgaat taacattggg
      481 cggatgacgt tatccagcgt gccaaccaca ccgctccaca ctaacaatac cgttccccag
      541 gtggtatcgc cagtccagta gagccagata atcgccggaa tcagtaccgg caacgggcca
      601 agctggacaa ggcaggagag gatcattaac accgttagca acgttgcata aggtacgccg
      661 gatacggcga ggccgatacc gccaagcact gcctgtacta acgccgtcac caccacaccc
      721 agcgccaccg cgcggatagc ctgtgccgcc agcagtacgg cggcatctcc gcgaacgcct
      781 gccagacggg ttgcaaaatg gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc
      841 agggcactga agagaagcat cagcgcacaa tgcaccataa agcgcccgat atgcgccgcc
      901 tgcccaacga accaggtggt ggtggtgcca atataagggc ggactttcgc catgatcgcc
      961 gtccccccca tatccagcaa gttgtgccag cctgcataca gcttcgcgcc aatcaccgga
     1021 atggtattaa gccacgccag atcgggtaac gtcatgtcgc cgctggaaat ggctttaatt
     1081 agcgggccgc tgccgtcgac gatactgtta accagcaaag caatagggat gataaacacc
     1141 atcactaata acagcgtcat caccagaacg gcgagagagc ggcggccaaa catgatcttt
     1201 tgcaaacgta acaataccgg ccaggtggcg ataaccaccg taccggccca tgcaaagccg
     1261 agaataaagg gttgaacaat ccacagacat gccacaatca tgatggctaa aaacagcacc
     1321 gaaagcagaa tttgtgcgac atccctgggc tgacgaacat ttaccataac tacttttcac
     1381 cttttttgtg cgtcaaatcg tcgacgcgac gtgaacacgc gaaactcacc tgcataatga
     1441 tgagcaattt cagcggtttt taacaggccg gttctgccct taattctgat gggcgtgtta
     1501 gaaaaaaatg tggtacaaca actcaggcaa cacgcaaacg atttactcgt cgtatttcaa
     1561 gttgcatgtg ctgcggctac gttcgttcac cccagtcact tactattgta agctcctgga
     1621 gttcactcgc ttgccgcctt cctgcaactc gaaatccata gagtaaataa acatcacctt
     1681 tcagtgcgct attgcagccg cctgaaagaa tagtgtatcg acctacatca cagaccgcag
     1741 gaaaggggca atataatgat tccacagatt tcccaggcac ccggcgtcgt tcaactggtg
     1801 cttaattttt tgcaagagct ggagcaacaa ggttttaccg gcgatacggc gacaagttat
     1861 gccgatcgtc tgacaatgtc gaccgacaac agtatttacc aacttctccc cgatgcggtg
     1921 gtatttccgc gttcaaccgc agatgtggcg ctgatcgccc gtcttgccgc gcaggaacgc
     1981 tattcatcgc tgatctttac cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc
     2041 aaccagggga ttattgttga tatgtcccgc catatgaacc gcatcatcga aattaaccct
     2101 gaagagggct gggtgcgcgt tgaggccggg gttataaaag atcaactcaa tcagtacctg
     2161 aaaccgttcg gctacttttt tgcgccggaa ctttcgacca gcaaccgggc aacgctcggt
     2221 gggatgatca ataccgatgc atccggtcag ggatcgctgg tctatggcaa aacgtcagat
     2281 cacgtacttg gcgtacgcgc ggtgttgttg gggggcgata ttctcgatac gcaaccttta
     2341 cccgtcgaat tggcggaaac gctgggtaaa tccaatacca caatcgggcg aatttataac
     2401 acggtttatc aacgttgccg tcagcaacgc cagttaatta tcgacaactt ccccaaactt
     2461 aaccgctttc ttaccggtta cgatctgcgt catgtcttta acgatgagat gaccgagttc
     2521 gacctgacgc gcattctgac gggttcagaa gggacgctgg cctttattac cgaagcgcgg
     2581 ctggatatta cgcgcttgcc taaagtgcgc cgtctggtga acgtcaaata tgactctttt
     2641 gactctgcgc tgcgtaacgc gccgtttatg gttgaggcgc gggcgctttc ggtagagacg
     2701 gtggactcaa aagtgctgaa tctggcgcgg gaagatattg tctggcattc cgtcagcgag
     2761 ttgattaccg atgtgcctga ccaagagatg ctcgggctga acattgtgga atttgctggt
     2821 gatgatgagg cgctgattga tgagcgggta aatgcactct gtgcgcggtt ggatgagctg
     2881 atcgccagtc atcaagcagg tgtcatcggc tggcaggtgt gccgtgagct ggcgggggtt
     2941 gaacgtatct atgcaatgcg caaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc
     3001 gctaagccaa ttccgtttgc tgaggatacc tgcgtaccgc cggaacacct ggcggattat
     3061 attgctgaat ttcgcgcgct gctcgacagc cacggcttaa gctacggtat gttcggtcac
     3121 gtcgacgcag gtgtcttgca cgtccgtcca gcgctggata tgtgcgatcc tcaacaagag
     3181 attttgatga agcaaatctc tgatgacgtg gtggcgctga ctgcgaaata cggtggtttg
     3241 ttgtggggcg agcacggcaa aggttttcgc gctgaataca gcccggcgtt tttcggtgag
     3301 gaactttttg cagaactgcg caaagtgaaa gcggcatttg acccgcataa ccgactcaac
     3361 ccagggaaga tttgcccgcc agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg
     3421 aagcgcggta cattcgatcg gcagatcccc attgcggtac gccagcagtg gcgcggtgcg
     3481 atggagtgta acggcaacgg tttatgcttc aactttgatg cccgtagtcc gatgtgtccg
     3541 tcgatgaaga tcacccagaa ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt
     3601 gaatggctgc gtttgttggc ggatcgcggc gttgatccac tcaaactgga acaagaactg
     3661 cctgaatccg gcgtcagttt gcggacgtta attgcccgca cgcgcaatag ctggcatgcg
     3721 aataaaggcg aatatgactt ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc
     3781 tgtaaagcgt gttcgaccct gtgccccatc aaaatagatg tgccggagtt tcgctctcgt
     3841 tttctgcagc tctatcacac ccgttattta cgcccgctgc gcgaccacct cgtcgctacg
     3901 gtcgagagct acgcgccgct gatggcacgc gcgccgaaaa cctttaactt cttcattaac
     3961 cagccgctgg tgcgcaaact ctcggaaaaa catatcggca tggttgattt gccgctgctg
     4021 tcggtcccct cgctacaaca acaaatggtg gggcatcgct cggcaaacat gacgctggaa
     4081 cagcttgaat ctctcaatgc agagcagaaa gcgcgcacag tgttggtggt gcaggacccc
     4141 tttaccagct attacgatgc gcaagtggtg gcggattttg tccgtctggt cgaaaaatta
     4201 ggtttccagc ctgtgttact gccattttcg ccaaatggca aagcccagca tattaaaggt
     4261 tttcttaatc gttttgcgaa gacggcgaaa aagacggcgg atttcctcaa ccgtatggcg
     4321 aagctgggta tgccaatggt gggcgtcgat ccggcgctgg tactttgtta tcgcgatgaa
     4381 tataaactgg cgctgggcga ggagcgtggc gagtttaacg tcttactggc gaatgaatgg
     4441 ctggcaagcg cacttgagtc acagccagtg gctacagtca gcggtgaatc atggtatttc
     4501 tttggtcact gtaccgaagt taccgccttg ccgggtgcgc cagcacaatg ggccgcgata
     4561 tttgcccgtt ttggcgcgaa actggaaaat gtcagcgtgg gttgctgcgg catggcaggg
     4621 acttacggac atgaagcgaa aaaccatgaa aattcgctcg ggatctatga gttatcctgg
     4681 catcaggcta tgcagcgact gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt
     4741 agccaggtaa aacgggttga aggcacgggg gtacgccatc ctgtgcaggc tttactggag
     4801 attattaaat gatatggaaa cggaaaatca ccctggaagc actgaatgct atgggtgaag
     4861 gaaacatggt ggggttcctg gatattcgct ttgaacatat tggtgatgac acccttgaag
     4921 cgacaatgcc agtagactcg cggacaaagc agcctttcgg gttgctgcat ggaggagcat
     4981 ccgtggtact ggccgaaagt atcggttccg ttgccggtta tttatgtacc gaaggtgagc
     5041 aaaaagtggt tggtctggaa atcaatgcta atcacgtccg ctcggcacga gaagggcggg
     5101 tgcgcggcat atgtaaaccg ttgcatctcg gttcgcgtta ccaggtctgg cagattgaaa
     5161 tcttcgatga gaaagggcgt ttgtgctgtt cgtcacgatt gacgaccgcc attttgtgac
     5221 gttgtcacca gaaaagtgtg acgcatattg cttttggtca ttatctgcac agtaaacggt
     5281 ggtatactgt acacgtcttg tctaaaagcg aggatgctat gtctactcaa cttgatccca
     5341 cccagctggc cattgaattt ttacgccgtg accagagcaa tctttcacct gcccaatatc
     5401 tcaaacgtct gaaacaacta gaacttgaat ttactgacct gcttactctc tcttctgctg
     5461 aattaaaaga agaaatctac tttgcctggc gtctgggcgt gcattgatcc tgacttaatg
     5521 ccgcaagttc gccaaatcga cctggattaa cctccgtcca ggttcgatga ccttcttaat
     5581 actgcttatc taccgcctga tattgttcaa aaataatact aatcgcagtt tgtgttaaaa
     5641 cggcgggtta gctttatgag attattctta tcgccccttc aagagctaag ccactgagag
     5701 tgccggagat aagcgccgga tggggtagaa acccttaagc ctgtgtcgca cagacttaag
     5761 ggttttctta tttctgaacg ctgcactttt ttacctatct cttaaatagt cattttaata
     5821 caactttttc tttttcatca cagccttaat gctccttaga taataaccat tatctaacaa
     5881 tgagatacct aattcttagc gtacctgtta acccacacat cagggtctat gcttattaaa
     5941 agcaggcaaa aagcatgttt tacggtaaag cccctgcgtt tgctgggttg aactgataat
     6001 cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa tcgatggaca
     6061 tgcattcagg aacctttaac ccacaagatt tcgcctggca aggcttaacg ctgacacccg
     6121 cagcggcgat acacatccgt gagctggtgg caaagcagcc gggtatggtc ggcgtgcgct
     6181 taggcgtgaa gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt gttagcgagc
     6241 cggacaaaga cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc ccgctgcaag
     6301 cgatgccgtt tattgatggc acggaagtcg atttcgttcg tgaaggactt aatcagatat
     6361 tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc tttggggtat
     6421 aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacct ggaccggcgg
     6481 cccgctgaat tataaagaag gattcttcac ccagttagcc accgatgagc tggcaaaggg
     6541 gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg agtggatgct
     6601 ggagtttcgt ctaaacgcct atcgcgcatg gctggagatg gaagaaccgc actggttgaa
     6661 agcgcactac gacaagctga attatcagga ttacagctac tactcagcac catcgtgcgg
     6721 taattgtgac gacacttgcg cgtctgaacc tggcgcggtg cagcaaactg gcgcgaacgc
     6781 ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg tgcgggaagg
     6841 caaagaggtg gcggtggatg ccattttcga ctctgtttcg gttgccacta cttatcgcga
     6901 aaaactggcg gagcagggaa ttattttctg ttcctttggt gaggcgatcc acgatcaccc
     6961 ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact tctttgccgc
     7021 gcttaatgcg gcggtagcct ctgacggtac gtttatttat gtgcctaaag gcgtgcgctg
     7081 cccgatggaa ctttccacct attttcgcgt taacgcggaa aaaaccgggc agtttgagcg
     7141 caccattctg gtggccgacg aagacagcta cgtcagctac attgaaggct gttccgctcc
     7201 ggtgcgtgac agctatcagt tacacgcggc agtggtggaa gtcatcattc ataaaaacgc
     7261 cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca ccggcggtat
     7321 tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa tgtcatggac
     7381 gcaatcagaa accgggtccg cgattacgtg gaaatatccc agctgcattt tgcgcggcga
     7441 taactccatt ggtgagtttt actcagtggc gctgaccagc ggtcatcagc aagcggatac
     7501 cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct cgaaagggat
     7561 ctctgccgga catagtcaga acagttatcg cggcttagtg aaaatcatgc cgacggcaac
     7621 caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt gtggggcgca
     7681 taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg aggcaacgac
     7741 atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca gcgaagaaga
     7801 cgccatctcg atgattgtta acggtttctg caaagacgtg ttctcagaac tgccgctgga
     7861 atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg tcggataagg
     7921 aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag ctatcctgcg
     7981 tggattaagc ctcgccgttc gtcccggcga agttcacgcc attatggggc caaacggttc
     8041 gggcaaaagt accttatcgg caacgcttgc cgggcgcgaa gattatgaag tgacgggcgg
     8101 cacggttgag ttcaaaggca aagatttgct tgcactgtcg ccggaagatc gtgcaggcga
     8161 aggcatcttt atggccttcc agtatccggt ggagattcca ggcgtcagta accagttttt
     8221 cctgcaaacg gcacttaatg cagtgcgcag ctatcgcggc caggaaacgc tcgaccgctt
     8281 tgattttcag gatttgatgg aagagaaaat cgctctcctg aagatgccgg aagatttatt
     8341 aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg atattttgca
     8401 aatggcggtg ctggaaccgg agttatgcat tcttgatgag tcggactccg ggctggatat
     8461 tgacgcatta aaagtggtcg ccgatggcgt gaactccctg cgtgatggca agcgctcatt
     8521 catcattgtt acgcactacc aacgcattct cgactacatc aagcctgatt acgtccatgt
     8581 gctatatcag ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac aactggagga
     8641 gcagggttat ggctggctta ccgaacagca gtaacgcgct gcaacagtgg catcacttgt
     8701 ttgaagctga agggacaaaa cgctccccgc aagcacagca gcatttacaa caattgctgc
     8761 gtaccggact gccgacacgt aaacatgaaa actggaaata tacgccgctg gaagggctga
     8821 ccaatagcca gtttgtcagc attgcggggg agatatcccc acagcagcgt gatgccttcg
     8881 cgttaacgtt agacgccgtg cggctggtgt ttgtcgatgg acgttacgtg cccgcgctga
     8941 gcgatgcaat tgaaggcagc ggatatgaag tgagcattaa cgatgaccgt caggggttac
     9001 ccgacgctat tcaggcggaa gtgtttctgc atttgacgga aagcctggca caaagcgtga
     9061 cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta atgtatatca
     9121 cccagggcgt ggcaggtgaa gaggtgaaca ctgcccatta ccggcatcat ctggatctgg
     9181 cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag ccttaatgat gctcgtcatt
     9241 ttaccggggc acggttcact atcaatgtcg cagcgaacgc ccacttgcag catatcaagc
     9301 tggcgtttga aaacccgctc agtcaccact ttgctcataa cgatttgttg ctggctgagg
     9361 atgccaccgc atttagccat agtttcctgc tgggtggcgc agtgttacga aacaacacca
     9421 gtacgcaact caatggcgaa aacagcacgc tgcggatcaa tagcctggcg atgccggtga
     9481 aaaacgaggt gtgtgatacc cgtacctggc tggaacacaa taaaggtttt tgtaacagcc
     9541 gacagttgca caaaactatc gtcagcgaca aaggccgtgc ggtatttaac ggtttgatca
     9601 acgtcgcgca gcacgccatc aaaacggatg gtcagatgac caacaataat ctgctgatgg
     9661 gcaaactggc ggaagtggat acgaaaccgc agctggaaat ctatgcagat gatgtgaaat
     9721 gcagccacgg cgcgacggtg gggcgtattg atgatgaaca gatgttctat ctgcgctcgc
     9781 gcgggatcaa tcagcaggat gcccagcaga tgatcattta cgcctttgct gccgaactga
     9841 cggaagcact gtgtgatgag gggcttaaac agcaggtgct ggcccgaatc ggtcaacggc
     9901 tgccaggagg tgcaagatga ctttttccgt cgacaaagtg cgggccgact ttccggtgct
     9961 ttcgcgtgag gtaaacggtt tgccgctggc ttatctcgac agcgccgcca gtgcacagaa
    10021 accgagccag gtgattgacg ccgaggccga gttttatcgt catggctacg cggcggtgca
    10081 tcgcggtatt cataccttaa gcgcccaggc gaccgagaaa atggagaacg tgcgcaagcg
    10141 ggcatcgctg tttattaatg cccgttcggc ggaagagctg gtgttcgtcc gcggcacgac
    10201 ggaagggatc aatctggtcg ccaatagctg gggcaacagc aatgtgcggg cgggcgataa
    10261 catcatcatc agtcagatgg agcaccacgc taacattgtc ccctggcaga tgctttgcgc
    10321 acgcgttggc gcagagctgc gtgtgatccc tctcaacccc gacggtacgc tgcaactgga
    10381 gacgctgcat acgctgtttg atgagaaaac tcgcctgctg gcaattactc atgtctccaa
    10441 cgtgcttggc acagaaaatc cactggcggg aatgatcacg cttgcgcacc agcatggagc
    10501 aaaagtgctg gtggatggcg ctcaggcggt gatgcatcat ccggtggatg ttcaggcgct
    10561 ggattgcgat ttttacgtgt tttccgggca taaactgtat ggccccaccg ggattggcat
    10621 tctttatgtc aaagaagcct tgttgcagga gatgccgccg tgggaagggg gcggttctat
    10681 gatcgccacc gtcagcctga gtgaaggcac tacctggacc aaagcaccat ggcggtttga
    10741 agccggtaca cccaataccg ggggcatcat tggtcttggc gcggcgctgg aatatgtttc
    10801 ggtgctgggg cttaataaca tagccgtgta tgaactgaat ctgatgcatt acgcgctatc
    10861 acagctggaa tctgtaccga atctcactct ctatggccca caaaacaggc ttggcgttat
    10921 tgcttttaat ctcggtaaac accacgccta tgatgttggc agttttctcg ataattacgg
    10981 cattgctgtg cgtaccggac atcactgcgc tatgccatta atggcctatt acaacgtccc
    11041 tgcgatgtgt cgggcgtcgc tggccatgta taacactcat gaagaagtgg atcgtctggt
    11101 gaccggcctg caacgtattc accgtctgct gggataacag ggaggcacta tggctttatt
    11161 gccggataaa gaaaaattgc tgcgtaattt tttacgctgc gccaactggg aagagaaata
    11221 tctctacatt attgagctgg gccagcgtct gccagaatta cgcgacgaag accgaagccc
    11281 acaaaatagc attcagggct gccagaatca ggtgtggatt gtcatgcgcc agaatgcgca
    11341 gggaattatt gaattgcagg gcgacagcga tgcggcgatt gtgaaagggc ttattgcggt
    11401 cgtatttatt ctctacgatc agatgacgcc gcaggatatt gtcaatttcg atgtgcgtcc
    11461 gtggtttgaa aaaatggcgc tcacccaaca tctcacccca tctcgttcac aaggtctgga
    11521 agcgatgatt cgcgcaattc gcgccaaagc cgctgcactt agctaaacta aagggacagc
    11581 tttcatccgg ttggctcaca ttgccggatg gtcaatgttt acactgacct gcacaggagt
    11641 gttttggttt caggtgaaca taagcgccat ccgacgtttc agcgtgagtc tccggcaaat
    11701 acaggaggtt tacaatgaaa cgcgcgtctt tgcttacact cacgcttatc ggcgctttta
    11761 gcgctatcca ggctgcctgg gcggttgatt atccgctacc accaaccgga agccgactgg
    11821 ttgggcaaaa tcaaacgtat acggtgcaag aaggggataa aaaccttcag gctattgccc
    11881 gacgttttga tactgcggca atgttgatcc ttgaagccaa taacactatc gccccggtgc
    11941 caaaacctgg tacgacgata actattcctt cacaactgtt attacctgat gcaccgcgtc
    12001 aggggattat cgttaacctt gcagagctgc gcctttatta ttatccgccg ggagaaaata
    12061 tcgtgcaggt ttatccgata ggtattggct tgcaggggct ggaaacgccg gtgatggaaa
    12121 cgcgtgtggg gcagaaaatc cctaatccaa cctggacgcc tacggcaggc attcgtcaac
    12181 gttcgctgga gcgtggcatt aaattactgc cagtcgttcc tgccggacca aataacccgc
    12241 taggacgtta cgcactgcgc ttcgcgcatg gtaatggcga atacctcatt catggtacca
    12301 gtgcgccgga ctgcgtcggt ttgcgcgtca gttcgggctg tattcgcatg aatgcaccgg
    12361 atattaaagc cttattctcc agcgtgcgga cgggaacgcc ggtgaaagtg atcaacgaac
    12421 cggtgaaata ttccgtggag cctaacggga tgcgttatgt tgaagtacat cgaccactat
    12481 cggcagaaga acagcagaac gttcagacaa tgccatacac actgccagca ggctttacgc
    12541 aatttaaaga caataaggct gtagatcaga agttagtcga taaagcgttg tatcgtcggg
    12601 cagggtatcc ggtttcggtg agcagtggag caactcccgc agccagcaat gcgccttcag
    12661 tagagtcagc gcagaatggt gaaccagagc aagggaatat gttacgcgcg aggcagtagt
    12721 ggtaaatagc agacaaaaaa aatggcgcac aatgtgcgcc atttttcact tcacaggtac
    12781 tattacttgc ggtatttagt agccatgttg tccagacgct ggttagcacg agctgcgtca
    12841 tctttagcag cctgaacgtc ggaacgcatt gcgttcacgt cgttgctcag ctggtcaact
    12901 ttagcgttca gagtctgaac gtcagaagac agctgatcga ttttagcgtt gctggagcaa
    12961 cctgccagca gagtagaacc caggattacc gcgcccagta ccagtttagt agctttcatt
    13021 attaataccc tctagattga gttaatctcc atgtagcgtt acaagtatta cacaaagttt
    13081 tttatgttga gaatattttt ttgatgggaa ggcacttatt tttgatcgtt cgctcaaaga
    13141 agcatcgaac gctgggtttt agataaaaaa attgagagaa aacaggatgc ttccatcgga
    13201 ttcatcttag ataaagtgag attatatagt gaaatccgat ttgatttttt attgcttctg
    13261 gttatcgatt aaataaaaaa agcgcccatc agggcgcttc gatatacaaa ttaattcaca
    13321 aaagcaatat tacaggacgt gaacagatgc ggtgttagta gtgccgctcg gtaccagtgc
    13381 accagaaacc ataactacaa cgtcaccttt gtgtgccaga ccgctctgca gagccagttc
    13441 tttacccaga cggtagaaat catcagtaga agtgatctct ttaaccagct gcggcacaac
    13501 gcctttgctc agtaccaact gatgagccgt tttttcgttg gtggtcagtg ccaggatggt
    13561 ggcatccggg aagtatttac gtactgcgcg agcagattta ccgccctgag tagcaaccac
    13621 gatcagcgga gcatccagtt tttcagcagt ttcaacggca ccacggcata ccgcttcggt
    13681 aatgcgcagt ttacggttgt cattgttgaa ctcgagacgg ctgttcatca cgcggtcggt
    13741 acgttcgcag atggtcgcca tgatagaaac cgcttccagc gggtatttac cttttgcgga
    13801 ttcaccagac agcatcactg catcagtacc gtcgaggatg gcgtttgcaa cgtcaccggc
    13861 ttctgcgcga gtcgggcgtg ggtttttgat catggaatcc agcatctggg tcgcagtgat
    13921 aacgacttta cgtgcacgga tacatttttc gatcatcatc ttctgggcga agataacttc
    13981 ttctaccggg atttctacac ccaggtcgcc acgcgcaacc atgatgccgt cagaggcttc
    14041 gaggatttcg tcgaagttgt tgaggccttc ctggttttcg attttggaga tgatgtggat
    14101 gttttcgccg ccgtgcgctt tcaggtgctc acggatttcg ataacgtcag aacgcttacg
    14161 aataaaggaa gcagcaacaa agtctacgcc ttgttcgcaa ccaaagatca ggtcctgttt
    14221 gtctttttca gccagtgctg gcagagcaat ggaaacgcca ggcaggttca cacctttgtt
    14281 ttcgcccagg tcaccgttgt tcagcacttt acagataact ttgttacctt caatggcggt
    14341 aacttccata ccgatcagac catcgtcaac cagtacggtg ttgccaactg acaggtcagt
    14401 agtgaaacct tcatacgtta ccgcaaccat ttcgctgttg ccgataacag atttatcagt
    14461 ggtgaaagta aaggtctgac cagctttcag agaaacgtcg ttaccgcctt ccagtttcat
    14521 ggtgcggatt tccggacctt tggtatcaag caggatagcg gcggttttac cagttttgct
    14581 catcacgttg cgcagattct gaatgcgctg accgtgttct gcatagtcac catgagagaa
    14641 gttcagacgc ataacgttca tgccagcgtc cagcatttta gctaacatct cttcagattc
    14701 ggttttcggt ccgatggtgc aaacaatttt ggtctttttc atgacagtct tagtctttaa
    14761 gttgagaagg atgggagaaa cttgctttct ggacgcactg ccggaaagcg aatccggtat
    14821 tacgaaagtg gtggtgcctc gctctaagga taggtgacat cgaaaaagcg tgcagaggaa
    14881 tgtgtgcttg tgtctcagcc caacgtgagt gcctgttttt gagtcgttga gttctacagt
    14941 ccaaagtgct gaaaccattc aagagtcaat tggcgcgcat tatacgctga aattaatcca
    15001 aaaggaaaac aaaaacagcg tttcaaaaaa tatacaactt gttagacaat aaattgttat
    15061 caaagattta acgttccagg gaaaaggtta cgcaaaaact tcgctgtttg cgcaacgtct
    15121 ggtgccaaag ttgtcagtgt gaggataagt gaaagttcat ccggtgtata aatatatcat
    15181 cgctcaattg ctacagttaa aataatttat cgcgttattt gtctgatttt gtttgatttt
    15241 ttgtttaatt ggttagattg atatatgctg atagttggca tgataaatag gtataaagaa
    15301 tgggaaatcg gacaaaagag gatgaactgt accgagaaat gtgcagagt
//
LOCUS       DAJTMK010000093        15073 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTMK010000093 DAJTMK010000000
VERSION     DAJTMK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15073
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.094"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            124..381
                     /locus_tag="OO135_002498"
     CDS             124..381
                     /locus_tag="OO135_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR5398306.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(431..1381)
                     /gene="uspE"
                     /locus_tag="OO135_002499"
     CDS             complement(431..1381)
                     /gene="uspE"
                     /locus_tag="OO135_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5398307.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1533..2285)
                     /gene="fnr"
                     /locus_tag="OO135_002500"
     CDS             complement(1533..2285)
                     /gene="fnr"
                     /locus_tag="OO135_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5398308.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2480..2995)
                     /gene="ogt"
                     /locus_tag="OO135_002501"
     CDS             complement(2480..2995)
                     /gene="ogt"
                     /locus_tag="OO135_002501"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5398309.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIDRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3006..4531)
                     /gene="abgT"
                     /locus_tag="OO135_002502"
                     /pseudo
     CDS             complement(3006..4531)
                     /gene="abgT"
                     /locus_tag="OO135_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            complement(4568..6013)
                     /gene="abgB"
                     /locus_tag="OO135_002503"
     CDS             complement(4568..6013)
                     /gene="abgB"
                     /locus_tag="OO135_002503"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR5398310.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGRISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQTQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSSLK"
     gene            complement(6013..7322)
                     /gene="abgA"
                     /locus_tag="OO135_002504"
                     /pseudo
     CDS             complement(6013..7322)
                     /gene="abgA"
                     /locus_tag="OO135_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            7498..8406
                     /locus_tag="OO135_002505"
     CDS             7498..8406
                     /locus_tag="OO135_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5398311.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPAQSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8736..9299
                     /gene="smrA"
                     /locus_tag="OO135_002506"
     CDS             8736..9299
                     /gene="smrA"
                     /locus_tag="OO135_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5398312.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGVQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(9320..10552)
                     /gene="dgcM"
                     /locus_tag="OO135_002507"
     CDS             complement(9320..10552)
                     /gene="dgcM"
                     /locus_tag="OO135_002507"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR5398313.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            10807..11790
                     /gene="zntB"
                     /locus_tag="OO135_002508"
     CDS             10807..11790
                     /gene="zntB"
                     /locus_tag="OO135_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5398314.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            12267..13640
                     /gene="dbpA"
                     /locus_tag="OO135_002509"
     CDS             12267..13640
                     /gene="dbpA"
                     /locus_tag="OO135_002509"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5398315.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(13769..14704)
                     /gene="ttcA"
                     /locus_tag="OO135_002510"
     CDS             complement(13769..14704)
                     /gene="ttcA"
                     /locus_tag="OO135_002510"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5398316.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILLNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(14756..>15073)
                     /locus_tag="OO135_002511"
     CDS             complement(14756..>15073)
                     /locus_tag="OO135_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5398317.1"
                     /translation="GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIR
                     RRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWINDMNDEQIAMLNA
                     RLS"
BASE COUNT         3751 a         3700 c         3792 g         3830 t
ORIGIN      
        1 attaatcaat gcgttgattg taaatccagc taagaggtga ggttttcaga gcagacaacg
       61 gtgaaatgtc atggtattgt tacgtttagg taacaagaaa tttgtctgca caaggattac
      121 atcatgatta tggcgaaact gaagtcagcg aaagggaaga aatttctctt tggtttgttg
      181 gcggttttca ttattgcggc gtcggttgtg actcgcgcga ctatcggcgg cgttatagaa
      241 cagtacaata ttccgctgtc tgagtggacg acatcaatgt atgtgattca gtcatcgatg
      301 atttttgttt atagcctggt ctttactgtg ttgctggcaa tcccgttggg aatttatttc
      361 cttggcggcg aagagcagta agtaaaaaat aggcccgata actcgggcct tgtcagttat
      421 tgaagagtcg ttaatcgtct tcttcgtcat ccagttcaac gggtgtctga tactggtcag
      481 gtttaatgac caacagatcg cagcgaagat gatcaatcac ctgttccgcc gtgttgccga
      541 ggaatgctgc tgaaataccg gtgcgtccta ccgtgcccag aaccacaatc cccgcctgta
      601 agtgctccgc caaatcagga ataacctctt cgggcagacc tttttctacg tgcgtcatgt
      661 tttcattaat gccgaatttc tgccgcaggg ctttcattgc cagcaaatgt tgcccacgaa
      721 tggcatcgtt ataaacgctc ggatcaaatt ccggcagttc aatcgcgata ttaattggcg
      781 ttactgggta agcgccaacc agatgaactt cggtatggtt gacttgttct gccagttcga
      841 tcgtctcttt gaccagtttt tcattgagcg cattatgata cggctcttca ctggcgagat
      901 tcaccgccac cagcgcctta cctccttccg gccacggctg gtctttcacc atccacaccg
      961 ggcttgggca tttgcgtaac agatgccagt ccgttggcgt aaaaatcacc gcttccagac
     1021 ggtcatgttg gtgcgccatt tttagcacca aatcgtgccc gccgctgatc acttcctgaa
     1081 tgatggcttc gaaaggacgg ttatgccaga ccactttaat ttcaatggga acgccagcat
     1141 tgagataata ttttgcctgc tcgtggatcc aggctgtacg ctggctgatg acgccctgac
     1201 gcatagcggt acgttcgtcc ggggagagca gggtggtcat ttcgtatgag aagtcataga
     1261 tcggcaaaaa ggctttaatt ttgccaccaa tccgttgatg taaataaaca gctcgccgca
     1321 atgctggttg gtcgtcctgg ttaggatcga taacaacgag catgttctga tacatagcca
     1381 tacagggtct ccttacaaca actgtcaacg cagtttgtaa ttaaaagatt aacccatatc
     1441 tggtgaatga aacagtgatg aaccttctgc cagatcaata aatcagaaaa atttaatgat
     1501 atgacagaag gatagtgagt tatgcggaaa aatcaggcaa cattacgcgt atgaccagca
     1561 agctgggcca gcgcatcgtt attttcgatg gtgatgtatt tacctttgac tgccagcata
     1621 ccgcttttct ggaagcgacc tagcagacgg ctgatggttt ctaccgtcag gcccagatag
     1681 ttaccgatat cgccacgagt catcgtcagg cggaattcac gaggggagaa gccgcgttgg
     1741 gcaaaacgac gggacaggtt gtagatgaat gcagccagac gttcctcggc atttttcttc
     1801 gacaacagca ggatcatgtc ctgatcgcct ttgatttcac cgctcatcag acgcatcatc
     1861 tgctgacgca gattcggcat tttaccggac aaatcgtcca gcgtttcgaa cgggatttca
     1921 cataccatcg aggtttccag cgcctgagcg aagctcgggt gatggccgct gccgatggcg
     1981 tcaaatccca ccaggtcgcc agctaaatgg aaaccagtga tttgctcgtc gccttgctca
     2041 gtgatggtat aacttttaat cgtaccggag cggatggcat aaagcgattt aagttcatca
     2101 ccagccttaa acagcgtctg gcctttctga ataggcttct tccgctcaat gatattatca
     2161 agctgatcaa gctcatgttc gttgagtgtg aacgggatgc aaagctggct gatgctgcaa
     2221 tcctggcaat ggatagcaca accgccagac tgaatgcgcc gtataattcg cttttccggg
     2281 atcataggtc tgctcaagcc gtaattgata tttgtcaatt ttaacatctt tttagggagc
     2341 aagtaagtct aagcaaacct taacagcaga gaattccgat attagatgta aatatatgtc
     2401 tatctatttg aaaaccatta agttgttaag ggtaacttta cataaaagtg tgaacaagct
     2461 ggcacaagtt gtttaatgtt tacagcaaaa gataaccttc gtggcgcaat aaccactctt
     2521 ttcgctgaac tccgcctgca tatccggtca tggtgccgtt tcggtcaata acccgatggc
     2581 aaggtacgac gatgctgatg ggattcgatc cgtttgccgc accaacggca cgcgccgcgc
     2641 cagggcggcc caattgttca gccagttggc cgtaatgcat aacctgcccg caggggatag
     2701 tgcgtagagt tttccagact tcgcgctgaa atggcgtccc ccctgtggca gtgggaagcg
     2761 tatcaataat gctaagatta ccggcaaaat aatcactaag cttgtcgctt aaaccgcctg
     2821 ggttggtggc agaaatgcgc tcatagcctt ctttgcgata atggatgtcc agcagctgca
     2881 ccatgcgttc gctgtactct tcccattcaa ccgcccgcag gcgaaattgc tcatcgcaaa
     2941 tcacccacag tggacccagt ggcgtggcaa ttttttcttc aagtaatctc agcatccgtt
     3001 ctctcttaag acaaacgtgg gtaaatcccc ggacctatcg gcagaccaac aagataccac
     3061 gccagcaaca tcagcagcca taccaccaaa aagataagcg gatagggcaa gactaacgaa
     3121 tagtaagtac ccagtttcgc gtctggtttg tagcgttgca ggaatccaag aaacagtgga
     3181 acaaaaggag ataccggcgc taaggcaata cggatgagtc ggcaatacga aagaggattt
     3241 gcgcaaatgc cgggtgaaag ccaagtagca taaacattgg tacgaaaatg ggggccagaa
     3301 tcgaccagat tgcggaacca ctggcaataa acatgcataa gaaagaggaa agcaacgcca
     3361 gaccgacaaa cgccgggatg ccgctaagcc ctgaactttc cagtatatcg gtcagcccca
     3421 cggcgatgaa tttccccatg ttgctccagt taaacatggc gacaaactgg gcgaggggaa
     3481 aaaccatcac gataaatccc gccatctctt tcatcggttc aatcattaaa tgcggtaaat
     3541 ccgcctgatg tcgaattgtg cgggtagcga tgccataagc cagcgaaacc acaaataaaa
     3601 aaagaatgat tagtggcacg atacctttaa taaagggtga tggcatcacg gtgtgattaa
     3661 tcggatcgcg caatatcccg ttttccggga tcaccatcag cgcgatcgca gcaataaaaa
     3721 gtagcgatac gacacctgct atgcgtaaac caaaacgctg actttcggtc aatgtctgca
     3781 gtttctcatc gctgtttccc tgccattgac ctaaccgtgg ctcgatgatt ttgtcggtta
     3841 tcaggccgcc aacaatcgtc agtacgacta cggagctggc cataaaatac cagttatcaa
     3901 ttacactgac gtgcatttgc ggattgaacg cagccgccgc ttccgtgcgg atccccgaca
     3961 gcaacacgtc ggttgtgaca atcagtaaat tagccgtaaa gccgcaacct acgcctgcaa
     4021 tggcagccag taaacctgca actggatgcc tgcccaccgc cagaaaaatc agcgcaccca
     4081 tcggtggcat gatcactaac gccgcatcgg aagaaatgtg gctgaaaaaa gcaataaaca
     4141 gcaccatata actggcgtag cggacattaa catgcgatgc cattttaacc attagcgctg
     4201 gcagtaagcc gacgcgctcc gccagaccgg cacctaaaac cagcgccagg atcgcaccaa
     4261 gtggagcaaa accgctaaag tttttaataa cattgggtaa aaaccagtgt aatccttcca
     4321 cactgagcag gtttttcacc accaccggcg ttccatcggt cgggtttttc gcactgacgc
     4381 caaaggccga caaaattgct gtcgtcacca tgagtacgat aatcaaatag ataaagagca
     4441 aaaaaggatg gggaaccttg ttaccaattc tttcgaccca gccatagagc ttcccggatt
     4501 gggaggacga cggtatggat gacatactca tgggcattcc tcggttgttg tgtttgcgtt
     4561 gttgttgtta ttttaaagat gacggtgtca cgttttttgg gatagggcag tgatacggtt
     4621 gcgtgtccgt aacttgctga tgctcttgtt ggcattcttg caatagccct gaatcaatga
     4681 agagattgag tgtggttgct gccatagttt tcgccgccag cagcattcct ttatgagcaa
     4741 tagatgttcg cccctggcta accagttgcc acgtatgtaa cggtgtaccg acggcaaaac
     4801 aggggctgaa acactgggca acaggcagtt tccaactgac gtcgccgaca tcagtcgatg
     4861 ccgcaagcac gttatcggtg gcggcatatg gagcgacctc attcgccagt accgtttcac
     4921 gatgacgtag tgcaaaagcc ttgccgtttt cgccaccggt tgcggcgata ttattcagac
     4981 tgttttgccg atcgttgggg gggagcgtag cctgagtttg tttcgtaaaa gccagttctt
     5041 cggagttcca ttccggggta ccaaaatggg atagggcatg gtacatggca ttttctaagg
     5101 tgcgattcgg gagataactg gaacaggctt tgtcgaagcg gcattcaacc gtggtttcgg
     5161 tcatcaatgc cgcaccttcg gcgattttgg cgacccgatc ataaatatgc tgcacgtcgg
     5221 tcatttcggg ggcgcggata agataaagca cttctgcctg cgcctggacc acgttgggcg
     5281 agatcctgcc gctatttgtg atggcatagt gtacgcgcgc tttttcaata atatgttcgt
     5341 tgaggaagtt ggtgccagtg gtcatcaacg ttacggcatc aagggcgctg cgtcccaaat
     5401 gaggggaatt cgcggcatgt gctgcgatcc ctttaaagcg ccatgatgcc tgaatgtttg
     5461 ccagcgtgcg ggtattgaac ataccggcaa aggcttccgg gtgccaggtg agtgccgcat
     5521 ccacatcatc aaataccccc tcgcgaacca tgaacgtttt acccgagccg ccttcttcgc
     5581 caggacaacc ataaaagcgc accgtgccgc cttgcccata ttgttccagc catttcttga
     5641 cggctattgc agcggcaaag gcggcggttc ccagcaaatt gtgtccgcaa ccgtgaccat
     5701 tttcaccggg cgtcacggat gtaggttgcg cgcaacctgc ttgctgactt aaacctgcca
     5761 gggcgtcata ttctcccagc agggcgataa ccggtttgcc ttgaccaaac gaagcaataa
     5821 aggcatttgg gatatagcct acgttgcggg taacggtgaa gcctgcagat tccagcgccg
     5881 aagccagatg ctccgctgac cagaactctt caaaacgtgt ttctggatga tcccagattt
     5941 gatcggcaat atcggtataa cgttggcgat cggcttcaat cgcatcgtcg ataaaacgat
     6001 agatttcctg catcagatac ctcgcgtcca gggaaaattg agcgcggtgc gcgccagcgt
     6061 ttcgacggca atagcgagaa cctgctcgtc aaaatcgaat ttttcgttgt gatgacctgc
     6121 cgccagctgt gtgccaaaca ccacgtagga ggcttgccct tgatgttgct gcacgcgggc
     6181 catcattaat gtggcatctt cggaacccgc aggcgcttca acacgttcaa tggcctgatt
     6241 gacccccgcg acctgagccg cctgactttg caaccatgcg acccattgcg gtgaaggaga
     6301 actggcggta gctgcaccca tcagacgagt ttcaacgccg acaccataca tggttgctgc
     6361 gccctgaatc gcttgttgtg cacggtcaaa aacatattga ttaatgacgt cgctggcccc
     6421 gcgtgtttcc actttcagca acgccgaggc aggaacaacg ttacgaccgc ttcctgcctg
     6481 cataacgccc acgtttactc tggaagctcc ttcgctgtgc ggggcgattg catgcagtgc
     6541 aagagtggct tgtgctgccg ccaacaaggc attgtgaccg tcttctggtt ttgcgcctgt
     6601 gtgagcggcg gtaccggtga agtgcgcgtc aaatttggtg gttgccataa aattatcact
     6661 gccgcacacc acggtgcccg caggtacgcc agtgccaatg tgcacggcag taaataatca
     6721 acatcatcta cgacacctgc atcgaccatc gcccgcgcgc cacgcgtacc ttcctctgca
     6781 ggctgaaaaa tcagtttgat gacgcaatgt agtccggact cgaattgttt aagggtatgc
     6841 gccagcccaa gcccaatggc ggtatgtcca tcatgaccac aggcatgcat cattccggcg
     6901 ttacatgacg caaaaccgtc gcggtagggg cgatggctga catcctgctc ttcactgaga
     6961 tccagcgcgt ccatatcgac acggaaagcc atcaccggac cggggcgacc ggtatccagg
     7021 gtggcgacga taccagtgaa accaccttca aaaaccgcaa tccattgtgc tagcgcaccc
     7081 tgttgacgag cgcgctcgaa ttcgcgttgt agagtgagtt catcaggtag tcccatccgg
     7141 ctactttcat taactacttc gcggcccagc gccagtgaat agccgagctg ctgcaattct
     7201 tccgcaacaa gggtggcagt gcggaattcc acccagccag actctgcata gtgatgaaaa
     7261 tcacgtcgcc agtgcgataa ttttggggca agcgaattaa caaattgatt caaagactcc
     7321 ataacctttc ccgtcatcag taaaaagtgt gacccggttc acgtagcgat agtttttact
     7381 tatcactaac tgatttttca cagttttaac cgttcataaa ttaccctgac acaatcatct
     7441 gcattaaagt agatgccagt ttctttggtc tgataaataa cggttatcgg tggcgtcatg
     7501 gcttttcagg taaaaattca tcaaattcgt gcttttgttg aagtggctcg tcagggcagc
     7561 attcgcggag cgagccgaat gttgaatatg tcgcaaccgg cacagagtaa atctattcag
     7621 gagttggaag aaggattagc ggcgcaactc ttttttcgcc gtagtaaagg cgtgacgtta
     7681 actgatgccg gtgaaagttt ttatcagcac gccagtctta ttcttgaaga gctgcgcgca
     7741 gcccaagagg atattcgcca acgacaaggg caactggcag ggcagattaa tatcggcatg
     7801 ggggccagta tttcccgcag tctgatgcca gctgtcatat ctcgttttca tcagcagcat
     7861 ccgcaggtaa aagtacgcat tatggaaggg caactggtgt cgatgattaa tgaattgcgt
     7921 cagggagaat tggatttcac catcaatacc tattatcagg gaccgtacga ccacgaattt
     7981 acttttgaga aattactgga aaagcaattc gcgatctttt gccgcccggg acaccccgcc
     8041 attggtgccc gttcgatcaa acagttactg gattacagct ggacaatgcc gacgccacac
     8101 ggcagttact acaaacagtt gagtgaattg cttgacgatc aggcgcaaac gccacaggtc
     8161 ggtgtagtct gcgagacgtt ctcagcctgt atcagtctgg tggcaaaaag cgattttctc
     8221 agcatactgc ctgaagaaat gggctgcgat cccttgcacg gacaggggct ggtgatgttg
     8281 ccggttagcg aaattttacc gaaagcggcc tattatttga ttcagcggcg tgatagtcgc
     8341 cagacaccac tgaccccgtc attaatcacg caattccggc gagaatgcgg ctatctgcaa
     8401 agttaaaact gcataaaaaa atagagtctg tcgacatccg ccagactcta cagtacacac
     8461 agcagtgcat ccgcgtctta agtccggcat tgtctcctct gcgccggtga ctgtgttata
     8521 acggttttaa cctttagttg ccaattttct tcagtgacag atttcacgaa aatataagtt
     8581 acattattaa tatcgtgaat gaataatcat gcataagcat tttgcttaaa atatcggcaa
     8641 tatttggaac ttattactgg aattttgggt aatacgttgt tggaccgacc cggtctggtt
     8701 atcatatcgc gctcttaatt gcgggaggat gtaacatgaa ccttgacgac aaatcgctgt
     8761 ttcttgacgc catggaagat gtccagccgc tgaaacgtgc tacagatgtc cactggcatc
     8821 caacgcgtaa ccaacgtgcg ccgcagcgta tcgacacgct gcagcttgat aatttcctca
     8881 ccaccggatt tctcgacatc atcccgctaa gtcagccgct ggagtttcgg cgggaagggg
     8941 tgcaacatgg ggtgctggat aagctgcgca gtggtaaata tccgcaacag gcgagcctga
     9001 atcttttgcg ccagccggtg gaagagtgcc gcaaaatggt gttcagtttc attcaccaag
     9061 ccctggcgga tggtttgcgt aatgtgctga ttattcatgg taaagggcgg gaagataaat
     9121 cgcatgccaa tattgtccgc agctatgtgg cgcgctggct gaccgaattt gatgatgttc
     9181 aggcatattg caccgcgcta ccgcatcatg gcggcagcgg ggcgtgttac gtcgcactac
     9241 gtaaaacggc gcaggcgaag caagaaaact gggagcgcca cgctaagcgc agccgttgat
     9301 ctcgagacgc atccgcggct tatgccgcca gcacgcggtt gcgtccatca tttttcgccc
     9361 gatacaaagc atcatcaacg cgtttaaaca gttcatcgat gctttcattt ccttcgtgat
     9421 gcgccacacc aatgctgacg gtaaaacgtg gtaagcccga aatactcact ttggccacgc
     9481 ttacacggat agtttcagcc agcgaaagcg cggtatccag tggggttctt ggtagcaata
     9541 agacaaactc ttcgcctccc caacgaaaca ccaaatcgcc tttgcgagcg caactttcga
     9601 gggtgcgggc gagggcgcat aacacctcat cacctttaga atgcccatag agatcgttaa
     9661 tgtgtttaaa acgatcggtg tcgatgagca acaagctgta atcctgggcg atggcgaggt
     9721 gctgcatttg gcctggttcc gtaatgtgat aaaactgtcg ccgattcagt aatccggtca
     9781 tcgcgtcatg gtgagcagca tgttccagct gctcctccag ccgtttttgc tcagtaatat
     9841 catgcacaat acataacatg agcttgtcgc cataaatttc aatcggcccg gcataggtct
     9901 gcacatgacg agtagatcca tccgccagtt tatgaacaaa attcaaaggt ttatgaccgc
     9961 cgggtaaatg cgaaatttca tgcatgatag gcatgacgcg acgcccgagc atattaattt
    10021 cccaggtatg tttctggcac atcgtttcat ggttataacc gtagaaattg agcgcggcga
    10081 ggttagcatc gacgatttgg ccatctcgtg aggggtcaat caacaacatt ggtgcagagt
    10141 tagtcagaaa aaagcgcgca taaaaacctt gttttttgcg ctgataattt gccgagcgac
    10201 tggcttttaa acccagcgtt gccggcgctt cgataccttc gaaaataatc accggttctg
    10261 tttctgtcag ctctcgcaaa acaagccgac agcttaatgc tgtttcctct tctttacgct
    10321 gaacagtgag gatttcgata atatcgtgtt ggttttgcag atcggagagg tatttcggca
    10381 gttctttttg tgaggagacg gaatagggtc cggttcgtag ctgactaaac gtgaggtctt
    10441 gcatcaacag tcgcgccgcg ctattggcat aaattaactg ttcctcaaag ggcgaaacaa
    10501 tccagacagg actggtgagt aagtccaggg tattgaagtt gtgcgtaatc attgagatcc
    10561 cgttattttt atcaattttt gttgctatcc gatcgcaaaa aagccacgtc atatgatcag
    10621 ataattctga taatgataga cgctatttaa cacttcacac ggtttgtata cggaaaagca
    10681 ttttgctttt tgtattcaat ttagacagaa ttttattaat catttcaggg taatggggtg
    10741 atgagatgtt gcgtaacagg gccagaaggc tagactacaa aataatgcgt tgatgatgga
    10801 ggcactgtgg aagcgattaa gggatcggac gttaatgtcc cggatgcagt atttgcctgg
    10861 atgctggatg gtagaggcgg cgttaaaccg ctggaaaata cagatgtgat tgatgaagcg
    10921 catccctgct ggctccacct taattatgta caccatgata gcgcccaatg gctggcgaca
    10981 acaccgctgc ttcccaataa cgtacgtgat gcgctggcag gcgagagcac acggccccga
    11041 gtcagccgtc tcggtgaagg cacgctgatt acattgcgct gtataaacgg cagcaccgat
    11101 gaacgccccg atcaactggt cgccatgcgt gtatatatgg acgggcggtt aattgtttcg
    11161 acccgacaac gcaaagtgct ggcgctggac gatgtggtga gcgatctgga agagggcacg
    11221 ggtccgaccg attgcggagg gtggctggtg gatgtgtgcg atgcgttgac cgatcattcc
    11281 agcgaattta tcgagcagct gcacgataaa attatcgacc ttgaagataa tctccttgat
    11341 cagcaaattc caccgcgtgg attcctggct ctgctgcgca aacaattaat cgtgatgcgt
    11401 cgctatatgg caccgcaacg tgatgtttat gctcgtcttg ccagtgaacg tttgccgtgg
    11461 atgagcgatg accaacgccg tcggatgcag gatattgccg atcgccttgg gcgcggtctt
    11521 gacgaaatcg acgcctgtat agcacggact ggcgtgatgg cggatgaaat cgctcaggtg
    11581 atgcaggaaa atttagctcg tcgtacctat acaatgtcat tgatggcaat ggtcttttta
    11641 cccagtacct ttctgacagg gttatttggc gtcaaccttg gtgggatccc tggcggcggg
    11701 tggcaattcg gattttcaat tttttgtatt ctgttagttg ttcttattgg tggtgttgct
    11761 ttatggttgc atcgtagtaa atggttgtaa caaaagcaat ttttccggct gtctgtatac
    11821 aaaaacgccg caaagtttga gcgaagtcaa taaactctct acccattcag ggcaatatct
    11881 cccttgcagg tgaatgcaac gtcaagcgat gggcgttgcg ctccatattg tcttacttcc
    11941 ttttttgaat tactgcatag cacaattgat tcgtacgacg ccgactttga tgagtcggct
    12001 ttttttgcct gttatttatc agcgtctacc ctttaagagt ccacccaatg accggaggga
    12061 aatatgacga cacttattta tttgcaaatt cctgtccctg aaccgattcc tggcgatcct
    12121 gttccagtgc ccgatccgat ccctcgcccg caacccatgc ctgacccacc acccgatgaa
    12181 gaaccgatta aattgtcgca tcgtgagcgt agatctgcga ggatacgcgc ctgctaactt
    12241 tgcgtcgatg accacgagaa tagattgtga ccgctttttc taccctgaat gttttgcctc
    12301 ccgcccaact cacgaacctt aatgagttgg gttatttaac tatgacgccg gtgcaggccg
    12361 ccgcgcttcc ggcgatcctt gccggaaaag atgttcgcgt gcaggcgaaa accggcagcg
    12421 gcaaaacggc ggcttttggc ctcggcttgt tacagcaaat tgatgcgtcg ctatttcaaa
    12481 cccaggcttt agtgctgtgt cctacgcgtg aactggcgga tcaggtggca ggtgaattgc
    12541 gtcggctggc gcgttttctg ccaaatacca aaattttgac gttgtgcggt ggtcaaccgt
    12601 tcggtatgca gcgtgattcg ttgcaacatg cgccgcatat tatcgtggca acgccgggtc
    12661 gtttgctgga tcacttgcaa aaaggcacgg tatcactgga tgcgctgaat actttggtga
    12721 tggatgaggc cgaccgcatg ctggatatgg gatttagcga tgccattgat gatgtcatcc
    12781 gttttgcgcc tgcatctcga cagacgcttc tgttttcggc aacctggccg gaagccatcg
    12841 ccgtaatcag cggacgagtg caacgcgatc ctttggcgat tgaaattgac tcaacagatg
    12901 ctttgccacc cattgaacaa caattttatg agacatccag caaaggcaaa attcctctgt
    12961 tgcaacggtt attaagcttg catcagccat cctcttgcgt ggtgttttgc aataccaaaa
    13021 aagattgcca ggctgtctgc gacgcgctga atgaagtagg gcaaagtgca ttgtcattac
    13081 acggcgattt ggagcaacgc gatcgcgatc agaccctggt acgttttgct aacggtagcg
    13141 cccgtgtact ggttgcgact gatgttgctg cgcgtggtct ggatattaaa tcgcttgagc
    13201 tggtggtgaa ctttgagctg gcgtgggacc ctgaagttca tgtacatcgc atcggccgta
    13261 cagctcgtgc aggaaatagc ggtctggcga tcagtttctg tgctccggaa gaagcacagc
    13321 gggccaatat catttctgac atgttgcaga taaaacttaa ctggcaaacg ccgccagcta
    13381 atagttccat tgtgccgctg gaagcagaaa tggcaacgtt gtgtatcgat ggcgggaaaa
    13441 aagccaaaat gcgcccgggt gatgtattag gtgcactgac aggagatatc gggcttgatg
    13501 gcgcagatat tggcaaaatc gccgtgcatc cggcgcatgt ctatgtcgcg gtccgtcagg
    13561 ctgttgctca taaagcatgg aaacagttac agggcgggaa gattaaagga aaaacgtgcc
    13621 gggtgcggtt attaaaataa tgaaatgttg aattgccggg tgcaagagta aacatcttct
    13681 tcggcattgc cggatgcgac gttggccgcg tcttatccgg cctccataag agtagcccga
    13741 tacgcttgcg catcgggcgc tatcctggtt atttcacttc aaccacattc agccgtaact
    13801 catccaactg attttcatct tcttctggct gccagcccgc cggttgtagt gggatctctt
    13861 cgcgatcaaa cgccagatca cccccgttaa ccacttcaga accgtgggtg atgcctttga
    13921 aatcgaacag gttggtatcg cacagatgcg acggcaccac attctgcatc gcgctgaaca
    13981 tcgtctcgat acgccctgga taacgtttat cccagtcacg caacatgtca gcaatcacct
    14041 gacgttgcag gttaggctgt gaaccgcaca ggttgcacgg aataatcggg aacgcttttg
    14101 catcggcaaa tcgctgaata tctttctcgc ggcagtaggc cagcggacga ataacgatat
    14161 gtttgccatc atcgctcatc agtttcggag gcataccttt catcttgccg ccgtagaaca
    14221 tatttaagaa caacgtctgc aggatatcgt cacgatggtg acccagcgcg atcttcgtcg
    14281 cccccagttc cgttgcggta cgataaagga taccgcgacg aaggcgagaa cacagtgagc
    14341 aagtggtttt gccctctgga atcttctctt tcacgatacc gtaagtgttc tcttcgacaa
    14401 tcttgtactc aacgcccagc ttttcaagat acacgggcag aacgtgttct gggaagcccg
    14461 gttgcttttg atcgaggtta acagccacca gcgaaaaatt gattggcgcg ctttgctgca
    14521 aattgagcag aatctccagc atggtatagc tgtctttacc ccctgagagg caaaccatga
    14581 tgcgatcgcc ttcttcaatc atattgaagt cagcaatggc ttcgcccacg ttacgacgta
    14641 gacgtttttg taatttgttc aggttgtatt gttctttctt tgtaatttgt tgattttctt
    14701 gcattatttc agttctctgg tactaaatgg ggcaaattgg gggcaaactt tgcaactacg
    14761 ataaccgcgc attcaacatg gctatctgtt cgtcgttcat gtcattaatc cacataccgt
    14821 aaatttcata caccatttgc gcagtttcat gccccatctg gctggctata aatgccgggt
    14881 tcgctcctgc cgtcaacagc cagcaggcaa aagtatgtcg cgtatggtac ggattacggc
    14941 ggcgaatacc agcacgtttt actgctgcat tccatcttgc acccaaactg cttaccgagt
    15001 aataaggttt ctgtttttcg ttacacaccc tgggcatgaa aacaaaatgc agtttttgct
    15061 tttcagttct gcc
//
LOCUS       DAJTMK010000094        14768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTMK010000094 DAJTMK010000000
VERSION     DAJTMK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14768
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.095"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            120..443
                     /locus_tag="OO135_002512"
                     /pseudo
     CDS             120..443
                     /locus_tag="OO135_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000487120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1427..2284)
                     /gene="repA"
                     /locus_tag="OO135_002513"
     CDS             complement(1427..2284)
                     /gene="repA"
                     /locus_tag="OO135_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5398318.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(2277..2351)
                     /gene="tap"
                     /locus_tag="OO135_002514"
     CDS             complement(2277..2351)
                     /gene="tap"
                     /locus_tag="OO135_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5398319.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(2575..2835)
                     /gene="repA"
                     /locus_tag="OO135_002515"
     CDS             complement(2575..2835)
                     /gene="repA"
                     /locus_tag="OO135_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5398320.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            complement(3075..3665)
                     /locus_tag="OO135_002516"
     CDS             complement(3075..3665)
                     /locus_tag="OO135_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5398321.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(3704..3913)
                     /gene="hha"
                     /locus_tag="OO135_002517"
     CDS             complement(3704..3913)
                     /gene="hha"
                     /locus_tag="OO135_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5398322.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(3959..4420)
                     /locus_tag="OO135_002518"
     CDS             complement(3959..4420)
                     /locus_tag="OO135_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5398323.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(4664..4876)
                     /locus_tag="OO135_002519"
     CDS             complement(4664..4876)
                     /locus_tag="OO135_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5398324.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            complement(5007..5567)
                     /locus_tag="OO135_002520"
     CDS             complement(5007..5567)
                     /locus_tag="OO135_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5398325.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWSGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            complement(5622..6368)
                     /gene="traX"
                     /locus_tag="OO135_002521"
     CDS             complement(5622..6368)
                     /gene="traX"
                     /locus_tag="OO135_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5398326.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            complement(6388..11258)
                     /gene="traI"
                     /locus_tag="OO135_002522"
                     /pseudo
     CDS             complement(6388..11258)
                     /gene="traI"
                     /locus_tag="OO135_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            complement(11245..11664)
                     /locus_tag="OO135_002523"
     CDS             complement(11245..11664)
                     /locus_tag="OO135_002523"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5398327.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTGDDGRTVRNGADR"
     gene            11746..11973
                     /gene="vapB"
                     /locus_tag="OO135_002524"
     CDS             11746..11973
                     /gene="vapB"
                     /locus_tag="OO135_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5398328.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            11973..12371
                     /gene="vapC"
                     /locus_tag="OO135_002525"
     CDS             11973..12371
                     /gene="vapC"
                     /locus_tag="OO135_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5398329.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(12380..14578)
                     /gene="traD"
                     /locus_tag="OO135_002526"
     CDS             complement(12380..14578)
                     /gene="traD"
                     /locus_tag="OO135_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCR5398330.1"
                     /translation="MSFNAKDITQGGQITSMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGRDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLYKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGIS
                     ESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         3193 a         4302 c         3698 g         3575 t
ORIGIN      
        1 cggtataaga atcgaccatg cagagagaga aaacaccgga gtggagggaa aagcagaaaa
       61 gctcacgtgg cataaggcgc ggacaggggt atcgcttagt ttttcaattt cctatacgtg
      121 aacaggcggc tgcattttta ggtgtcgttc ccgttgaaaa atattccggc acctcggtca
      181 gagggtgtcc gaaactgtca aaaattggtc cgccggaaat ccgggcgaaa ctgtatctgg
      241 cttcgttgtg tggtctgaga tttaacccat tgatgaaagc gatgtatgag cgattatgcc
      301 tgagaggtaa ggccaaaatg tgtgccattg gtgcgctgat gagaaaactc gtgcactggt
      361 gctatggcgt actgaagacc caaaaaccgt ttgatgtggg ctatttgtct tcatatgcgc
      421 gtagcgcatg taaggcggca tgatgtagtt acatatggta caggagtaac accgccgaag
      481 cccggcgtaa gccggtacgt attgataggt ttcacctatc ccattcctgc cctgcgaggc
      541 catgcgggct ttcagcgatg atgaagtata ggtaagccct tttaaccccg ttagcgcggc
      601 tatgccgcgc tgtatcgggg acttagtgct gacagcttgc tggcagtact tagggggctg
      661 tacgttggag ttcaagcgat ttttctcgac agaacacgat aactgccaag ccatacccgt
      721 tttcagccat gacatagctt ctgtgcgcgg tcggagccca acaaaggttt acccgcagtc
      781 ggggcgtatc tccgtgttag cgggccgtga gggccgctta cgggcgtgta ctcgatacgt
      841 ccagtcagag gactgacagc gatgataata tagttacaac attcataatt aaaagcgact
      901 ctgttccggc ccttcgggcc ggggcggggc cgcttttcag ttatgaggga ggggctttgt
      961 ggtttcagtt ctgcgttgga cttgggtttt ttagggggtt atgtttgtat gttgtaacta
     1021 aaccagcttc ggtcggggcc cgccgcttgc ggtgggaggt gcatatctgt ctgtccacag
     1081 gacaggcagt gaacaggttt tctttttaaa tgaatgtaat taagtagttt aaaggagata
     1141 caaacaggtg tttaaaagat acattgcacc ctgtagggct gacggctggc gctttatgac
     1201 gtgaacggtt gtaaccttat ggtgaagtcc cttgcagttt aatgtggata agcaaaattc
     1261 cccgtctgtg aggcgtgttt tgtatcaaaa acagggggga ccggatgtac cagaaggtgg
     1321 atgatgaggt tgttttttgt atgtagcgct gattttttgt gcactggcgg gcttcaggcg
     1381 tgcgaatgcc tccggcgcgt gccggattat tctgaggagg tcactttcag ggggaagctg
     1441 tggccagccg gctgtaatta cggttacgtg acagaatcat gcgctccttc acacgacgct
     1501 ccacttcacg ttttaccgcc tcacgactgg cagtgaagcg cccttccgag atttcacgcg
     1561 tcagttgccg tttcaccagg gtgacaatat cctgacgctg cctgtccgca tcacgacgcg
     1621 cacgggcacg cttcattcca cgggacttaa gctctgtctg gtaactgcgg aaacgctcac
     1681 gaacaaaacg ccaggctttc gccatcagtt catccatacc cagggtatcc agcccctgct
     1741 ttttgcgctg tctgttttcc cattccacac gactgcggcg cgcggcggcc actgcctcct
     1801 ctgatacatc gagggcagca aacagtgcag atgtgaacgt gatatcggtc ggaatgtagc
     1861 acccgataag cgggtcatat tccgtctggt aggtaatcag tcccagctct gcaaggaacg
     1921 tcagggcccg ggtggcccgg gtgatggaga gttttccggc agcagactcc gtcgccagtc
     1981 cgcactcaat ggccagcgtg gtgatggaac actggacgcg gttggccagt gggtcatagt
     2041 gaaaacacag cccctgcagc agcgcatcaa tggcccggcg acgtagtacc ggtggcatgc
     2101 gccgacgcag accaagcgaa cgggcatgtg ccacatgaat ggcgaaatca aaacgggagg
     2161 tgaagcccac cgccttttcc atcagtttcc cgcagaacgg aagcgtccgg gcaccttcac
     2221 ggggtgtgaa caccggattc gggttcttta cctggcggta ataagtttgt tgaagatcag
     2281 tcacaccatc ctgcacttac aatgcgcaga aggagcgagc acagaaagaa gtcttgaact
     2341 tttccgggca tataactata ctccccgcat agcttgattg ttggctatac ggtttaagtg
     2401 ggccccggta atctttctga ctcgccaaag ttaaagaaga ttatcggggt ttttgctttt
     2461 ctggctcctg tacatctaca tcagaaccag ttccctgcca ccttatggcg tggccagcca
     2521 taaaattcct taaacgatca gtaatctagc atgctacgcc acaaagtaaa gtctttactt
     2581 tagtatatcc agtctctgca gttcatcttt gatgattttc tcaacgaact gagcctgtgt
     2641 tatcccctct ctctcgcagt actcaaccat gagatctttc agaggatttt tgacaaaaac
     2701 ttttatctct ttgtgtgtaa gacgttttct tgcaacagcg gccatttgtt tctcagagtc
     2761 agtcataggc ttacctctgc gcacaaaccg cttttgactc aatgaggaag tcactgcatt
     2821 ttctgtctgc gacatctcgc ctcctcaata ctcaaacagg gatcgtttcg cagaggatac
     2881 tacagttttt tgaaatcagc gacttgagaa ttgtggcgaa gatctgtgtt ggcgcacaaa
     2941 tcaacgggga ttactgtcgt ttaatgtgat ttaaactgtg aaatagtatg gttttcagtt
     3001 atggaaacgc cgggagcaag gaaaacttgc tttttcccgt ttccggggtt ggacaactga
     3061 gcaacgcgaa ggcgtcagct acgatgttcc ggtgactgct gatcagcccc tgttgtattc
     3121 agccattctc cggtcatctg cagcagtttt ctggcatcgt ggcttctgag caacggaata
     3181 ccagcctgcc tcagaacctc ttccagaaga atatcccggc gtctgcgttc cggtcgtaaa
     3241 tggctggcat catccagctc tactgctgca acaatactga acgaacgcag ctcaacgata
     3301 accacatcaa catgccactg agacaccatc ctgaataact gccaccactg gcgtgatcgt
     3361 ggccggatat tcccgttcag ctggacgata tccgcgaccc gtacctgcgg acacagatac
     3421 caccgcttca tatccacaat tctgaacagt cctttcataa aactgacttc cctttctgtc
     3481 agcagcgtct tctgacgttg ccagtattca ccgctggaca gtattcgctc tgcatcaccg
     3541 gtccgtatac ctgccgcatg caattgcatc agcaaaggag aatgctgatt tacacttctg
     3601 aggataatcc ggataagtag cgctgcaata atcagaacaa tgagaataaa aataatcaac
     3661 ttcattattt atctgctcct ggcaattaat tattgctgaa ttattacctt acatatttcc
     3721 atatctcttt tggtatcctg tcatataact ttccggtgat taattccgct agccggtggt
     3781 ctgctgctga attaaatgat tcacgttcac tgtttgataa actgtctcta tttttatgaa
     3841 taattttttc cagcgtattg actgatgaac atcgccgtaa ctgatacagc cattcctgtt
     3901 ttgtttttgc catttttatt cccgctgaga acagatgaat gataggattg ccatcgcttt
     3961 atttacggtg cctccatatc caggggggaa ccggatcagc atctgaccag agcccacgtt
     4021 tttgttgtct ggcttcgttc tgaaggacag gtagcacggg atctgtgttg tactgctcat
     4081 atacccaggc agccccggcc cggaccataa actgattaat attcattccg tctggtgcat
     4141 aaacctgccc gagaatacga ccataacgat ctcgctgaaa atatgtgacg gtaaccgttt
     4201 ttcctgcgac cagtgatttc atcatgtctg ttgaccaccg gccataatcc tgtttctttt
     4261 ccggtgcatc aatatttacc agcctgatcc gcaccgcctt cagtgagtcc atgacctcga
     4321 tggtatcacc gtccagtaca cgaacaaccc gtccatgaat atctgcagac aataccggcc
     4381 atgaaaagat aaataatacc agtggaatat atttcctcat gttattctcc atatcagata
     4441 aatggcacct gtatttcagc ccccctgaat agtgcagcac acattgaata acaccttatt
     4501 ttgtgaaatt aatcacaaaa tataaaaaat cgacttaatg cggaaaagac tctgtgctac
     4561 tcagaactgt ccggtcatac tgcccgaccg gatattaaga agcaaatctc ataaactcct
     4621 ttcagccttc tgactcctgg agtaatgaag gctgcttttt tcgttacccc cttccccggc
     4681 agcgccggtg gcaatgttca gcccttttcc gggcaaactg ctgccagtct gtcctaggcg
     4741 caacacaggc agcccgtcgc catgcttctg ccgcttccat ccagcgtcca ctgacttcca
     4801 gaataacggc ccgcccgccg gcacgctggt aacgccagac ctggccgttc cgtgaactgt
     4861 ttctgaatcc attcattttc ttgtccccct ttagttgtca gtattattgt gataaataat
     4921 tcagtggact ggcttccttt cggaagcgga agttatttcc ttttaagcct tatttccctg
     4981 cctcctctct ctgggggagg ctttttttat ttctcatcaa gcacggcctg aagttctgcc
     5041 tttatccggt tctggcgacg aattttcgcc agacgcgctc ccgcatacgc ttcctcctcc
     5101 tgagaaatat gctccgtcac atacccttcc gtgtcatacc ggcaggcacc ggctttcatg
     5161 gcacacagat agctttctga acgggtgatg gccttcagcg ccctgcgcag ttttttatgc
     5221 gagagcggga tattccgctg cgccacgtct tccagtaaca cgtcccggat accgcaggcc
     5281 agaagccggg gcgtgtcacc gtcaaataat cccgaccacc agggcttcag ggtattcaca
     5341 gcctcatcca gcgtgggcag gttcagataa atggacagcg cctgtctggc ctgcgctttt
     5401 tttgccgcca gctctgcttc acgggccgct ttctcggcca gcttctgctt tttcaccttc
     5461 cactttggtg gcgtggtgac attgatgatg gttttccggc tgcggacagt cgctgttccc
     5521 tctgtcttcc gcttcagtgt cagtaccggt cgcttctgct ctgtcatgct gtgttctgcc
     5581 tctgacgaat catcctcttg cggccaaaga taccacaccc gtcacagcgc cagaacgccc
     5641 agcacagcca gatgacaggc atagaacgtc gggaaaaagt ctccgggcca gaaacggggt
     5701 aatgattttc cggcacacaa caccagacca acggtcagca ccgtcatcac aagaccggcc
     5761 accgccgctg catcactcgt ggcaaggtta agcgccggaa taacagccag caggcaggcg
     5821 accagcgcca gacgttccgc tctgtcttcc gcccggtaca gccggttgct gactgccagc
     5881 atcagcaacc cggcaatacc gtaactggtg ccggacaaag gcccccacag ggccatcagg
     5941 agtatggccg ctgctgtacg ccacccactg cgcgtttcac accacgtcag cacctgtgcc
     6001 gccactgcaa aggcaaacag gatattcccc tcataccagg gaaagcctgc cagataatat
     6061 gcgaactggg caataattcc ccatccccac agccggttaa tggccggttg ccggatatgc
     6121 gcatgacggg acagattcag cccccacacc agggcaaaaa gcggaaaggc tcctcgcccg
     6181 gccagaaaca tccattcctg cttcagctgg aatatcagat tgatgtggtc cagtaccatc
     6241 agcaccagtg ccaccgtttt gatgatatcc cgctgtccgg gtgaccagac cagaaatgac
     6301 tgcaaccagg tatcggtccg tgcagccagt gaatcgttac gtgtcgtgtt cgtgttatcc
     6361 gttgtcatca gtgtttgtct tcctgtatca gtctccgccc ggggttttct ctttctgcag
     6421 gtcacgaacc atatcccgct ccatctgctg cagacgctcc tgcagcaaat ggagcgggca
     6481 acttcccgga ccgcttcccg cacccgttgt ggttcacgca gtacactctc cggcagcgcg
     6541 gcttcccgtt cagttatggc agcccggtca cgctcctgcc cggcaatttc cctgacagcc
     6601 tgctctgttt tgccgtccgg caggtcaggt ttgttctctg ccattttacg gacaatttca
     6661 tctgcgcgac gttccgtttc acggcggatg gcctcttcag cctgccgctg tgccagtacc
     6721 tctgccgtga ccggttcgcc atttccggct cccggctgga cgctgttgtc cgggatatcc
     6781 ccccacacgc ggccaccggt gatggcaccg ggattccacg gacggccttc accggcgatt
     6841 cttaccacca caccactgtc aggattatca cgggcaatcc ggacgccatc ctgcatatta
     6901 tcagccagca ggctttctcc gttacggctg ccctgcaggg ccacgaactg cgcatccccg
     6961 ctgcctttta cccgaccttc accgctgaat ccccgcagcc cgtttccgtc atccgtggtc
     7021 agcgggttca gccagatacc ggcggacttg ccgttacggt caaacgccgg cagtgccaca
     7081 tacggttgcg gatatttacg tcccggagca ataaaccgtg caggactgtc tcccccggcc
     7141 agccccgcct gacggagaac ggcacggcct gccgccacgt cccgcagctc ccgcgccgta
     7201 ctgaacagcc gctctgcatt catgacctcc cggtccggtt tcggctcaaa cacatcgtgg
     7261 gcagttcctt tctgtacggc attgttgatg gcatccgtcc agccctgacg gttatcggtg
     7321 tacacctgca catgctgctt catacgcgac agggccacgt aggctgactc aaagccggcc
     7381 atcagtttcc ggttaccttc cgtgccttca agcgcgatgg caaaggtttc actcgccccc
     7441 tgtgcaccgt gggcggtgat ggcataggcc aggtcaatat gttgctctgc ccgctcctga
     7501 ccggggcgaa tcacacgggt ctgctgaccg tcagaaagtg tgacactgtc accggaaacc
     7561 gctgtcaccg tccagacgct gttggccaca taaccgcgct cccggtcact cttcgtgaag
     7621 cgtatcctgt caccggtccc cacccggatg gtgtccgggg tgtacagcgt gacgccttca
     7681 gccaccgcct cccggggcga aatcagccgc gtgttacctt ccgcatcctg cagggttatc
     7741 agcccgtcat ccttgctgat accggcaatc cggtgataca cattatccac cagggcaagg
     7801 gcatccgggt tattctccca ggtggagaga cgacgcagct ccccgtcgcg tatattcgct
     7861 gtgttcagga caggcaccat gacctgcacc tgccccagtt ctccggcctt ttctcgtgca
     7921 tcatgaatca tgctgttcag tacgcgccgg tcctcattca ggtgcgtgac aatcagcgtc
     7981 tgctcccgtg cttccggtgt cctgccggta tagtcgcgga caatggcttc atacagtgtc
     8041 atggggacat ccggaaaagc ctcgcctttc agcatcgcct tctgctgcgc ttctgccagt
     8101 ttcgcttcct ggctgtgact gaactccgtc acggagtgct ccggtgccca tgcgccctcc
     8161 agacgtggca cctgagacgg tttcacacgc tcaagcccgg acagcgccct ttccacatcc
     8221 cggttaatca ggctgtatac cgcctcccgc agttccggcg tctgacgcac aatctccttc
     8281 atgatgacca catcggcagc actgcgcgtc tgctggagac ggaaaggctg accgggcgcg
     8341 atggcctgca gctggtccgt gtcaccgctg gccacagccc gaccgccacc ggccgcaatc
     8401 agggcgtatg cccgtgccat gtcggtattg cccaccatag agctctcatc gagaaggaac
     8461 agcgtgttgc tgaaatccgg cgtttctccg ctgcgctgct gcagctgcat gtcatgcaga
     8521 aaggacgcca gtgtctgtgc atccacgccg gcgctgcgca tctccccgac cgcacggtgt
     8581 gtgggcccca gccccacgac acggggacgt ccactctccg gcagcatgtt cacggctgac
     8641 atcaccgccc ggaactgtgt ggtcttaccc acaccggcat agccctgcac caccgtgaaa
     8701 cggtcggacg tttccagtat catacgggtg gcggcacgct gtcctgatgt cagtttctcc
     8761 atgagttcgc caggtactct ctccatcagc ggcgtgaccg cctccttacc ttcgagaata
     8821 tggcgaagaa tgctcttttc tgcctcatac gacgcacggg aaaccagcag gcctgtgcca
     8881 tagccttttg ccacatccac atacagtaaa tcaccccgtt ttatctgcgc attgatttcc
     8941 cctcccaggt cagcaaaacc ggttccttct gcagcaaacg actttgcctc tgtcagcagg
     9001 tccaccatgc tgaaggccag gtttttactt tccagcacag gaagggccag atgaatggcc
     9061 tgctgtgccg gcgtgtgaag cccggctttc tgcagactga tagcggtttc cagcaatgtc
     9121 tcaccggcac gcgcctttat ctgctcagaa accaccgtaa aggagggatg gcgggcaagt
     9181 ttttccgcag tacgggtttc atccagtgag gaatacagcc ggacatcacg accactgcgg
     9241 gccagaccgt tcagggtggc attgtccatt gccatctgtg tgacggaggc aaaaaccgtc
     9301 gcactgtcgc tgacggaatg cccgggcgtt tccacccagc cgttctccag cttcagtgcc
     9361 gtgaacggtg aatcgctcac aggcagggtg gccggttcag cccgtcccgg cacaacaacc
     9421 gtcatcgcat cttcactgac ggatgccacc tgcaggcggt caccgccgga gacgcggagt
     9481 ccgggaattt tccctgtcac cctcagacgc tcaccgtctg ccaccggcat tttttccggc
     9541 cggaacagcg accagctgct gtccagggaa gaaatacgca ccacctgcgt ttcgccctgc
     9601 gcattccgca gggtcaggct gtgactctgc gccgtcacac gttctgtcac atagcggtca
     9661 tgactgcgtg tctccgggtt ccactgctcc atcaccattc ccgggcggta catatcccgc
     9721 agataacggc tccggctgtc cagccagacc ggtgacaggg cggtcatggt tacctccggg
     9781 tgtccgagca cgccctgtgt tttcagctca ctgcgaatgg cctgtgtcag tatggcctgt
     9841 tcccgtactc cgctgacctg tgccacgctc tcttctccgg ctttcacgct ggccgcaaaa
     9901 tctccggcca gccgggcata gcggacataa cggtccggtt cactgatgat ggtggccggt
     9961 cgctgttctc ccccctgcca gcgatatgtg ttcaccccgg catccttcat ggccatcagt
    10021 gcactgcctg taccggttcg ctgcccgctg tcagttatca ggacctgtac gttatgacgt
    10081 gcggcaccat ccagcagggt taacgtctct ttcagggaga gtttttcgcc ctggtcgacg
    10141 ataacggtac tgcccggcgt gaaggccatg ccttccagca actgacgacg tccggttatc
    10201 agttcaccgg acagccgttc atcctgcttc aggttcatct gcgagcgacg gtcagcagcg
    10261 ataatctgca cctcccgccc ctgctcccgg gccatcatga ccagttcagc cacccgctca
    10321 cgctgcccgg ctgcaccgcc ctgcccggac acaatggcca gcgacggacg gtcctgtgcc
    10381 agaacactga cggcatcgct gtaaccggcc gtccggggga cacttttctc cggatgtacg
    10441 gtcacccggt tctgtttcat gatgtcacga ctgagtgccc ggactgacag ctcatcgagc
    10501 atatgaatcc cggacgtgaa cagccccttc tcacgatcga gggggataag ctgctcacgg
    10561 ctgatggcct catcgatacc ggcgcgtgcc cgttcaatca caccattttc cggcggcagt
    10621 atgccgaccg tcctggccag caggtccgta tacataaact gcactttgcg ttcacttaat
    10681 ccggcaatgg cctgtgtcac cgcctgctgc acatccggcc cgtcctgtga agcgggtccg
    10741 ggtgtctgcg tgcgggtata tgcccgttgt tccgctgcgt cacgatatgc ccggatgtcg
    10801 aacccggttt ccttcagcgt ctgcatccac tcggccattc tgacttcagg atcgacgtgc
    10861 tgtttggatt tacgcgtatc cagcgccgcc acatcccggg atttcagcga ggcgtcttcc
    10921 ccgacggcct cccggatagt ctgtgagcgt ccggaaaagg cctccaccgg tacacccggc
    10981 atttcccaca taccgtgctt accgaccact tcagtttcat agcccagcgc ctcaacctgc
    11041 tctttcagtt tttcccggta gagcctgcca aaggcaatct gattagcgta cacattctca
    11101 atgaaccccg ttttccccac tttgtcactg ctcagtgtct tccattcgcc gttatgctgc
    11161 gtaacattag tcaccaccgc atgcgtgtgt aactgtggtt cctgatcgcg actggtgtcg
    11221 tggttaaaca gtgccatcac cagattaccg gtcagcaccg tttctgactg tccgtccgtc
    11281 atcacccgtg tggaggccag tgcctccaca cgggtgatga cggacagcaa aatccacggc
    11341 ctggttatgt gcttcaataa gacgtttgtc ccccccgagc atggccatca tggagacact
    11401 tttgggagcg gagaaggtca ggtcgtagcc gggacgatgc ctgttactgc catcctgcat
    11461 gcggcttaaa tccgccccgt ccggcagcct gccctccaga aggcgggtaa agacatcctt
    11521 atcgacactg ccctgcagcc ccagttgttc agccccctgg ccggcccagc gttctcccat
    11581 gctgcccagc acatagtaat tatccttgtc ggtataataa ttaccggcac ttccggccga
    11641 tctgacctgc gcgatactca tcataaacgt atatcccttt gacatatccc ggtatcaatc
    11701 ccacaatgga tatacacaag acatatccac ataaggaggc aaataatgga aaccaccgta
    11761 tttctcagca accgcagcca ggcggtcaga ctgccaaaag cggttgcatt gccggaaaac
    11821 gtaaaacgcg ttgaagtgat tgctgtcgga cgaaccagaa tcattacgcc agccggagag
    11881 acatgggacg aatggttcga cggacacagc gtcagcgccg attttatgga taacagggaa
    11941 cagcccggca tgcaggaaag ggagtcattc tgatgctgaa gtttatgctc gataccaaca
    12001 tctgcatttt tacgataaag aacaaacccg ccagcgtcag ggagcgtttt aacctgaacc
    12061 aggggagaat gtgtatcagt tcggtcaccc tgatggagct gatatatggt gcagaaaaaa
    12121 gccagatgcc tgaacgtaat ctcgctgtga tcgagggatt tgtttcccgc attgatgttc
    12181 tggattacga cgctgctgca gccacacaca ccggccagat aagagcagaa cttgcccgtc
    12241 agggacgccc tgtcgggcca tttgatcaaa tgatcgcagg tcatgcccgc agtcgggggc
    12301 tgattattgt gactaataac acccgggaat ttgaacgtgt gggcggcctg agaactgaag
    12361 actggagctg acctgttcat cagaaatcat ctcccggctc aacatcctcc ccgcgttccc
    12421 ggtgcacatt gatgttcacc tcttcacgac gctgcatctg ctgccaggtg tccggatgat
    12481 tttcctgctg ccatgcctca taagcggcca tatccaccac ttcaccggat tcactgatcc
    12541 cgggtggcag ttgctgttcc atctcttctt ccggtttcat tttcagctcc tgctcgatcc
    12601 cccctgccgg aacattcaca cctgaatctg acttcttatc gttgatgaca ggagacaccg
    12661 gctgttgcgg ctgttgcggc tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt
    12721 cagcctgagt cacgtcttct ccggaaacaa cctccgggac atccggttcg aagaggctgg
    12781 ccatctgacg accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt
    12841 ccgggttgat gtcacgcgga ataaactccg gagcgacctt cggtcgttcc tgatatttca
    12901 gtgagagttt gactgccgga tacggtccgg gcagggtgac atagcaggtc agatccggca
    12961 gggactgaat gtcggaataa ctgaccagcg tctggcgctc catatcctta ccggtcgata
    13021 ccccgtcacg taccggatca gcaccgtagg aatactgttc gctggctttc aggtgctctt
    13081 tctcaccaat ttcaccggca gcgaactccg caatcttatg gctgggagaa cggaaaaagg
    13141 cacgggtgtt catgacgtca aacagcgtgg cagccgcttt ctcaccgtag atatcttcca
    13201 gctgggcata ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca
    13261 ggatctccac caggtccggc agtttgtata atgtgggtaa ctcatcacag aagaaccaca
    13321 cacgacgatt acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca
    13381 tggagatcac cggcttcagg gaggcatgag tgtcggcatt cgacgagata aacagccagc
    13441 cgtttttctg atcctcccgg acaccacgca tccagtcacg gatggtgaag ggctcaccgt
    13501 tatgctcaat cccctgcagg taacggatgg ctttcacata gttggtcagc acagcacgga
    13561 tggatatggc cgttttctcg attttctctt ccaccaggtt ggccgccggt gaattacgca
    13621 ggtaggtacg cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc
    13681 tgcggttggg gtcattacgc atcagatacg ccgcttccgc aaaaatggta cgtcctgaac
    13741 cctgccagaa cgggtcctct tttgtgccca tcgggatcag agtatttgcg gtattatcaa
    13801 aatccggctg tgtcaggcac tccttccaca aatcccaggc ggcacagcgt gcatccagcg
    13861 gattcaggat cttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt
    13921 cataaatcac caccatatca ccacgctgac gggcgtagtt ggccagacga cggataacct
    13981 ccgacttacc ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga
    14041 taatcggcag atcgccaatc cggatatcgg agtccctgcc gtcttttttc agcatccggg
    14101 caacgtcttt cggattgtct gtcagttgac gaccacctgt gacttcattt tcgctctgtt
    14161 gtttaccctg acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca
    14221 gtgccacaac tgtggccaga acgaatgcag accataactg ctcaccgcac cagatcatat
    14281 atttatcatg cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactgaatct
    14341 catataccgg ctgagacttg attaaatccc gcatgccttc cagcgtggta caccaccagt
    14401 aaatacagcc attcacaaac gtctgccagc ttatttttac ccataaaacc agaccaacga
    14461 gtatccagaa aaaaataaac aggcagtaaa gcataatatt ggcgatctgg ctgaacatgc
    14521 ggatacgcat ggacgtaatc tgaccgccct gggttatatc ctttgcgtta aaactcatga
    14581 tgatattccg aagaaagaaa gtcagtcgga ctgattatta atgatgaaaa gattaataac
    14641 cgttaaattc cggcttcgcc ggcaacactt cacggtcgca caaagaattt gcacaaccgc
    14701 tcagtgtttt catatttatt tcggaaacgc cggggagaaa taaaatttat tttatttctt
    14761 tccggggt
//
LOCUS       DAJTMK010000095        14759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTMK010000095 DAJTMK010000000
VERSION     DAJTMK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.096"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..430
                     /locus_tag="OO135_002527"
     CDS             <1..430
                     /locus_tag="OO135_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR5398331.1"
                     /translation="RGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
                     KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGIT
                     GADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ"
     gene            complement(431..622)
                     /locus_tag="OO135_002528"
     CDS             complement(431..622)
                     /locus_tag="OO135_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5398332.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(727..963)
                     /locus_tag="OO135_002529"
     CDS             complement(727..963)
                     /locus_tag="OO135_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5398333.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1081..2520)
                     /gene="rsmF"
                     /locus_tag="OO135_002530"
     CDS             complement(1081..2520)
                     /gene="rsmF"
                     /locus_tag="OO135_002530"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5398334.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     ALKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(2600..5233)
                     /gene="yebT"
                     /locus_tag="OO135_002531"
     CDS             complement(2600..5233)
                     /gene="yebT"
                     /locus_tag="OO135_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5398335.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPRNKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGGKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5202..6485)
                     /gene="yebS"
                     /locus_tag="OO135_002532"
     CDS             complement(5202..6485)
                     /gene="yebS"
                     /locus_tag="OO135_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5398336.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVELLDSRLLWDAHESGNARFDD"
     gene            6615..7112
                     /gene="msrC"
                     /locus_tag="OO135_002533"
     CDS             6615..7112
                     /gene="msrC"
                     /locus_tag="OO135_002533"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5398337.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIKDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7209..7907
                     /gene="proQ"
                     /locus_tag="OO135_002534"
     CDS             7209..7907
                     /gene="proQ"
                     /locus_tag="OO135_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5398338.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7927..9975
                     /gene="prc"
                     /locus_tag="OO135_002535"
     CDS             7927..9975
                     /gene="prc"
                     /locus_tag="OO135_002535"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5398339.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDAFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPVEQPAPVK
                     "
     gene            10167..11048
                     /gene="htpX"
                     /locus_tag="OO135_002536"
     CDS             10167..11048
                     /gene="htpX"
                     /locus_tag="OO135_002536"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5398340.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11094..12467)
                     /locus_tag="OO135_002537"
     CDS             complement(11094..12467)
                     /locus_tag="OO135_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398341.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            12644..13435
                     /gene="kdgR"
                     /locus_tag="OO135_002538"
     CDS             12644..13435
                     /gene="kdgR"
                     /locus_tag="OO135_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5398342.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13578..13817)
                     /locus_tag="OO135_002539"
     CDS             complement(13578..13817)
                     /locus_tag="OO135_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398343.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13976..14119
                     /gene="mgrB"
                     /locus_tag="OO135_002540"
     CDS             13976..14119
                     /gene="mgrB"
                     /locus_tag="OO135_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5398344.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14194..14481
                     /locus_tag="OO135_002541"
     CDS             14194..14481
                     /locus_tag="OO135_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5398345.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVILIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3879 a         3590 c         3780 g         3510 t
ORIGIN      
        1 aagaggcaat catgaacaga tggcgatgga tgcgctggca tcccagcaga tgtctttgtg
       61 gttgatgaat ggcggcgact ggtttattgc gctggcagat aatcaacaga aacaagcgaa
      121 aacggcgctg gaaaaatgtc agcatttgcc ctttattctt gaagtacaca gtcgcactgg
      181 caagcatgtt attgctcatg cggattatcc agatgatgtt tatgaatggc aaaaggacgt
      241 tgatttgcat caggtcttgt ggagccgctc gcgattaggt gaacgccaaa aagggcaggg
      301 aattacaggt gctgatcatt tctggtttgg tcatacaccg ttgcgacatc gcgtggatat
      361 tggcaacctg cattatattg ataccggtgc tgtctttggg ggcgaactga ctcttgtgca
      421 attgcaatga ttaaaaatca ccgtactcct gtgcaggtcg ccagaaacca tctataaaat
      481 cctcaatcgg aaaacaaccg ccctggcgga tccgttgatc gctcatagaa taaagacact
      541 gctgttccgt gttgtagaca tccacaacaa tatcttcaca accgccatcc aggtagcaaa
      601 caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg tgccgtaccg ttaagtttag
      661 gagagatttt acaacggggg aataaccagg acaaataacc cgccatgccg acgggttctt
      721 tttggatcag gcaagacgca taatccaggc atcagattgt tgatcgtaac gggtacggta
      781 aaggacagag tgttcgccat ttagcagggc agggatactg gtttcagcat cccaggcatc
      841 gagttgcata cataaatcgg ggtcagattt acaaggaatg gttaacgttc gatcacccgg
      901 cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg cctatgcgca cactggtttt
      961 catcttcgtg ctctccattt agctgccggt tgtggtacga ccagtccgat cgcaccattt
     1021 ttcagcctag ttgaggcagc cgaatgtgcc agtcaaaaag tgcgcttttt ttgtgcgctg
     1081 tcaggcgtta ccggtaaaaa gtttcccatc gcgcaccagt tcacgcggat agctgttttt
     1141 caatcgcgaa ccaatccgtt tggctaaacc aatcggttga tgctggaaag taaccaacac
     1201 gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc ccgcgatacc actcctccgc
     1261 ttcctgcggt gtcagttcaa aagcgttcac attgtcgggg gtggcaaggg caataactgc
     1321 ttcatgctgc cagcgataac ctttgttgtg cgtttcggca agtttaatcc ccaagcgaga
     1381 aaatcggact ttaccgatca gggcttcaat gcccaccggg aacaaccaca gttctttgtc
     1441 acgctgccag aggcgcaggt tttcatccca gtttaagcca acacctgcag ccgcctggcg
     1501 aatttgtcca gcttcgcgat ctttcaccgg gctgaacgga aaattaccga ctttgtattt
     1561 gggggcgggt aaggcgggaa tcgcctgagt tttacgcaga cgagcaacga aaaagccttc
     1621 gcagtcgtaa atttgtggga aaacatgcaa aaagccttct tcggtcagcg ctttgtttgc
     1681 accagggaag agatcgccaa gtggtaaaaa ctctactgcg tcggggtaag tctctttcag
     1741 ccacaggcaa acggcttcgt tttcttcctg gtttaaggta caggtcgagt aaaccagcgt
     1801 accaccagga cgtaatgcat gaaaggcgct gtcgataagc tcccgttgtg tagctgcgat
     1861 ttcctgattg ctttctggtg accagttttt tagcgcatcg ggatctttac gcaccacgcc
     1921 ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac atttctggca ctgccgcacc
     1981 aaacacgcgg ccatcaaaat gtgtgagcgc aacattactg atgccacagc ggctgatatt
     2041 ggcatgtaac acttttaccc gactggcgga aaactcattg gcaaggattg ccccttcgtt
     2101 attcatccgc gcggcaattt gcgtcgtttt tgagcctggc gcagcagcga catccatcac
     2161 ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg ggcaacattg aactggcttc
     2221 ctgaatataa aacaggccac ttaaatgctc ggcggtacta cccaatggca atgcatcttc
     2281 attgtcgcgt tcaatccaga aaccttcttc acaccacgga attggcgtaa gcgtccagcc
     2341 ataaggagcg gttaattgca ggaaatcagc aacggagatt ttcagcgcat taacgcgaat
     2401 gctgcggcgc aacgggcgct gacaggcggc aagaaaatca tcaaatgaga gcgtcgaagg
     2461 catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa taaacggtgt gttgggccac
     2521 gagcatgtac cactggcaaa aaaacaggcg cgcagtttag cataaacgct ccggcgcagg
     2581 cacgccggag cagtgggcat tatttgggaa gcgcagttcc ccattcacgc cactctttcg
     2641 gttcactttc ctgcaacagg aagtgtttgc cttcctgggc tttcggtgcc agtggcgtac
     2701 ccggaggcgt ggcgaaggcg atgccgccac ggataaactg gttaaaggtg ccggttttca
     2761 ctacgccgcc cgtcagacca aagtccagac tgtaacccga tgccaaccag aagacggaat
     2821 tgttacgcac caggtgttga tagcgtttac tgatgcgcat cgcaatcatc acgcgatctg
     2881 acaatgtccc cagcgtcatt ccggtaaccg tacctacttc cagaccacgg aacagcacag
     2941 gcgtaccaat acctaacgaa ccggcttccg gtgcttcgac aataatgctt aagccatcca
     3001 ggtaacgcga atcagtaata gtagcttctt gcaactcaaa gtcacggcga ggattacccc
     3061 gacctggttc gacattgata tacggctgga ggatagtatc tagatgctca acgcccgcgg
     3121 ctgaaatttg cggtgtgacc accgagaagc gcgtaccgcc gcgggcgaag gtctggacat
     3181 actccggata gagcaccgcc tttgcctgta cttcattgcg cgcggtaatc agatccagcg
     3241 tctggatttg cccgatatca ataccgagat agcgaattgg catcccgacc gccagttttc
     3301 cggcatcgaa agcgtgaagc gtaatctgcc caccaaccgc acgggccgct gtttcggaag
     3361 catacagaat tcgtttgtcg cctttacgct gactggcgct ggcaccgctg aggttatcga
     3421 agctaatggc tccctttaat gctctggaga gcggggatgc ctgtacggtc aggccactac
     3481 cattcagctg aactttcccc ccgccttctg cccagaacac gctattgctg gtcagaaggt
     3541 tgcgatactc cggcttaata tgcagatcga tatcaaacgc gttagctcgc ggacgcacgg
     3601 taatcacttc accaacttca aatttacggt acagcactac cgatcctgcc tgcacatccg
     3661 gcagcgtctc tgcactcaaa ctcacggttg tggtgggtaa atcgctaagg ctgttctcca
     3721 gcgctttttc cagattggca tacagtggat agctggcttt catctcacct ttattgcgtg
     3781 gcagaatacg tatcccgccg ttaatccatt ctgaggcgct ggcaccaaga aactcaacgc
     3841 catccagccc caccttcacg tcgacacggc tgttgacgac aaatttgcta tcgcctttta
     3901 ccagttcgcg atgctgaggc tcgatggcga cggtaaaggt aacgcctttg ctggtgagtt
     3961 tacgatcaat aacctggcct acctgcacgc cgtgaagaat gagcggctga cccgtatcaa
     4021 taccgtaact ttccggcgcg gtcagggtca gcgtcagaac atcaggttca tgcagcagtg
     4081 ctttttcgcc tggcacaaca acgaactctt tgcgtggctc gccatcgccg ggtaccaact
     4141 cgaaggtttt gccagtcagc agggcgctga gattggcatc gctaagggat aatttcggat
     4201 tacgtaattc gatgcgggta ttttcacgaa gtagggtaac gacgctggga tcaactgtca
     4261 tttccccagt gactttacca ccaggattta aatccagttt agttagctgt ccgacttcca
     4321 gtccctgata cattaatggc gtcgagtcgg cggttaatcc ggccccactc ggcagttcca
     4381 gtttgattat tacgccacgc tggctatggg cgaggtcttc atacagacca aaggtatctt
     4441 ccgcctcggc aggtttcgac tcttctggtg aatcgaaggc aatcgcaccg ttaaccagtg
     4501 ccgccaggct ttccagtttc accttcgcgc cactgatact gacattggca tcaacgccgg
     4561 aaacgttcca gaaacggcta ccttttttca ccagatcggt aaaacgccgc tcgatcagga
     4621 catcaatcac cacgccttgt ttgttgggat tgatggcata gtcgtagact tttcccaccg
     4681 ggatcttgcg gaaatagacc aatgaaccgc tgttcagcga accgagatcg ggggcttgca
     4741 ggtggatcat cagatcgcca ttgtccagcc gatatttcgg ttgggtatcg agtgcgacaa
     4801 agtgatcctg ctctttacct gtacccggca tcatgccgat atagttccca ccgacgaggg
     4861 cgtccagccc ggagacacct gccaacgatg cttttggcgt caccagccag aattgagtct
     4921 cttcgcgcag cgcatctttc atatcggact tgatgctgac cttgacttca atcttacgaa
     4981 gatcgtcgct gaggctgata tcctgcactg ttccgacttc aacgccctga taacgaacag
     5041 gcgtacggcc cggtacaata ccatccgccg acataaagtc gatggtgacg gtattacttc
     5101 ggtcctgata actgtcccaa atcagccaac cggcaatcat tagcgcgatg aaaggcagca
     5161 gccagaaagg tgagatacgg cgtttatttt taatctgcgc ttcagtcgtc gaagcgggcg
     5221 tttcctgact catgtgcatc ccaaagtaag cggctgtcca gcaattccac agcaagaata
     5281 gtcaaaatta cggctgcgcc aaaataaaac gcagccggtc ccatagtaaa agcgaggatc
     5341 tgatcgcgat taatcagcga catggttaaa gatatgacaa acaggtcgag catcgaccag
     5401 cgtccaatcc aggtcaccat ccgcagtaac agaatgcgtg tgcgtaaacc ttgctggcat
     5461 ttaaaatgaa tgctcaacag taaagtgaac atgacgatca ctttagtaaa cggtaccaga
     5521 atactggcga taaacacgat tccggcgacc gcaatgttgc tacttgccag cgacataatc
     5581 ccggaaagaa tcgtatcttc ctgccggcca ccattcagat aaatgataga aataggcaat
     5641 aggttggctg gtaacaacaa aacgattgac gctaacagcg ccgcccagca tttttgcaaa
     5701 ctatgacgac gacgcaggcg tagcgggata tggcaacgcg ggcagcgacc acgctgatca
     5761 ggatagccgg taaaatggca cccaagacag acacgaagtt tctcgtccct acgcgtagcg
     5821 gggcgctgcg gataatatcg ctcccacagc tcctcaacat taagatgtga caacgtcacc
     5881 gtcgttaaaa tcaccaacgc cacaaaagag aacaagccga cacccgcctg gatatgggca
     5941 taatcctgta cctttataga agcaacgcca atgccgacca ggtagatatc cagcattacc
     6001 cactctttaa gacgctcaag catcagcagt accggacgta aattcattcc cagtcggtta
     6061 ccaaaccata aatatgctat ggaggacacc agaatgaggg gggcaccaat aacgcagaaa
     6121 aagaccatcg cccccgttat cgtatcgccc tgtttggtca tttgccagat gccttgcatg
     6181 acgtttgcgt cgatacgaat acctaacagc cagatatgca acagcggttc gccccaggca
     6241 aacggcatca gcaacagcat ggtgaaagcc attgccgcca ggcgcgttag cgaccagtcg
     6301 cgcccgtcgc gaatttttgc ctgacagcgc ggacaatagg cactctgatg agaatttatc
     6361 tcgggcaggc taaacagcat gtcacattgc gggcaacgtt ggtaatcacc acgcggcagt
     6421 tcctcgccga ttgacctcac tgttatcttt tttgtcggcg taatttgtgg tgtgttaaga
     6481 gccatgacgt gccaaacgta ataagaatta acattaatct taacccatga tgggaatgat
     6541 cttgagcatt aacgcattta atgcttattt taataggctg gcagttccag gtaagacaaa
     6601 caagtgatta tataatgaac aaaacagaat tttacgcgga tttaaatcgc gactttaacg
     6661 cgctgatggc gggagaaacc agttttctgg caacgcttgc gaacaccagt gcgttgttat
     6721 acgagcgtct cactgacgta aactgggcag gtttttattt gcttgaggac gatacactgg
     6781 tactcggacc atttcagggc aaaattgcct gtgtccggat acccgtcggg cgcggggtgt
     6841 gcggcactgc ggttgcccgc aatcaagtgc agcgtatcaa ggatgttcac gcgtttgacg
     6901 ggcatattgc ctgtgatgcg gcgagtaatt ctgaaattgt tctgccgctg gtggtgaaaa
     6961 atcagattat tggtgttctt gacatcgata gtaccgtctt cggtcgcttt acagacgagg
     7021 acgagcaagg cttacgtcag cttgtggcac agcttgaaaa agtgcttgca acgacggatt
     7081 acaaaaaatt ctttgcgagc gtcgcaggat aatcaacgga taacgtagca tttactggta
     7141 gcgtcattat aatgacgcct gttcatgcct gcgcttgttg gctacgtccg ttgtaatcag
     7201 gaaatttcat ggaaaatcaa cctaagttga atagcagtaa agaagtaatc gcgtttctgg
     7261 ccgaacgttt tccccactgt ttcagtgcgg aaggtgaagc gcgtccgctg aaaatcggta
     7321 tttttcagga tttggtcgat cgtgttgccg gggaaatgaa cctgagcaaa acgcaattac
     7381 gatccgcttt acgtctctac acttcgagct ggcgttatct ttacggtgtt aaacccggcg
     7441 caacgcgtgt cgatcttgac ggcaacccat gcggtgagct ggacgagcaa catgtagagc
     7501 atgctcgcaa gcagcttgaa gaagcgaaag cgcgtgttca ggcacagcgt gctgaacagc
     7561 aagcgaaaaa acgcgaagct gccgcagctg ctggtgagaa agaagacgca ccgcgccgcg
     7621 aacgcaagcc acgtccgacc acgccacgcc gcaaagaagg cgctgaacgt aaacctcgtg
     7681 cgcaaaagcc ggtagagaaa gcgccaaaaa cagtaaaagc acctcgcgaa gaacagcaca
     7741 ccccggtttc tgacatttca gctctgactg tcggacaagc tctgaaggtg aaagcgggtc
     7801 aaaacgcgat ggatgccacc gtattagaaa tcaccaaaga cggcgtccgc gtccagctga
     7861 attcgggtat gtctttgatt gtgcgcgcag aacacctggt gttctgaaac ggaggccggg
     7921 ccaggcatga acatgttttt taggcttacc gcgttagctg gcctgcttgc aatagcaggc
     7981 cagaccttcg ctgtagaaga tatcacgcgt gctgatcaaa ttccggtatt aaaggaagag
     8041 acgcagcatg cgacggtaag tgagcgcgta acgtcgcgct tcacccgttc tcattatcgc
     8101 cagttcgacc tcgatcaggc attttcggcc aaaatctttg accgctacct gaatctgctc
     8161 gattacagcc acaacgtgct gctggcaagc gatgttgaac agttcgcgaa aaagaaaacc
     8221 gagttaggcg atgaactgcg ttcaggcaaa ctcgacgctt tctacgatct ctacaatctg
     8281 gcgcaaaagc gccgttttga gcgttaccag tacgctttgt cggtactgga aaagccgatg
     8341 gatttcaccg gcaacgacac ttataacctt gaccgcagca aagcgccctg gccgaaaaac
     8401 gaggctgagt tgaacgcgct gtgggacagt aaagtcaaat tcgacgagtt aagcctgaag
     8461 ctggcaggaa aaacggataa agaaattcgt gaaaccctga cacgccgcta caaatttgcc
     8521 attcgtcgtc tggcgcaaac caacagcgaa gatgttttct cgctggcaat gacggcgttt
     8581 gcgcgtgaaa tcgacccgca taccaactat ctttccccgc gtaataccga acagttcaac
     8641 actgaaatga gtttgtcgct ggaaggtatt ggcgcagtgc tgcaaatgga tgatgactac
     8701 accgttatca attcgatggt ggcaggtggt ccggcagcga agagtaaagc tatcagcgtt
     8761 ggtgacaaaa ttgtcggtgt tggtcaaaca ggcaagccga tggttgacgt gattggctgg
     8821 cgtcttgatg atgtggttgc cttaattaaa gggccgaagg gcagtaaagt tcgtctggaa
     8881 attttacctg ctggtaaagg gaccaagacc cgcactgtaa cattgacccg tgaacgtatt
     8941 cgtctcgaag accgcgcggt taaaatgtcg gtgaagaccg tcggtaaaga gaaagtcggc
     9001 gtgctggata ttccgggctt ctatgtgggt ttgacagacg atgtcaaagt tcaactgcag
     9061 aaactggaaa aacagaatgt cagcagtgtg atcatcgacc tgcgtagcaa tggcggtggg
     9121 gcgttgaccg aagcggtatc gctctcaggt ctgtttattc ctgcgggtcc cattgttcag
     9181 gtccgcgata acaacggcaa ggttcgtgaa gacagcgata ccgacgggca ggtgttctat
     9241 aaaggccctc tggtggtact ggttgaccgt ttcagtgctt cggcttcaga aatctttgcc
     9301 gcggcaatgc aggattacgg tcgtgcgctg gttgtgggtg aaccgacgtt tggtaaaggc
     9361 accgttcagc aataccgttc attgaaccgt atttacgatc agatgttacg tcctgaatgg
     9421 ccagcgctgg gttctgtgca gtacacgatc cagaaattct atcgcgttaa cggcggcagt
     9481 acgcaacgta aaggcgtaac gccagacatc atcatgccga cgggtaatga agaaacggaa
     9541 acgggtgaga aattcgaaga taacgcgctg ccgtgggata gcattgatgc cgcgacttat
     9601 gtgaaatcag gcgatttaac ggcctttggg ccggagctgc tgaaggaaca taatgcgcgt
     9661 atcgcgaaag atcctgagtt ccagaacatc atgaaggata tcgcgcgctt caacgctatg
     9721 aaggacaagc gcaatatcgt ttctctgaac tacgctgtgc gtgagaaaga gaataatgaa
     9781 gatgatgcga cgcgtctggc gcgtttgaac gaacgcttta aacgcgaagg taaaccggag
     9841 ttgaagaagc tggatgatct accgaaagat taccaagagc cggatcctta tctggatgag
     9901 acggtgaata tcgcactcga tctggcgaag cttgaaaaag ccagacccgt ggaacaaccc
     9961 gctcccgtca agtaatatca atcaggcaca agaaattgtg cctgattttt taacagcggc
    10021 aagatgccgt aaatcagatg ctacaaaatg taaagttgtg tctttctggt gacttacgca
    10081 ctatccagac ttgaaaatag tcgcgtaacc catacgatgt gggtatcgca tattgcgttt
    10141 tgttaaactg aggtaaaaag aaaattatga tgcgaatcgc gctcttcctg ctaacgaacc
    10201 tggccgtaat ggtcgttttc gggctggtac tgagcctgac agggatacag tcgagcagcg
    10261 ttcaggggct gatgatcatg gccttgctgt tcggttttgg tggttccttc gtttcgcttc
    10321 tgatgtccaa atggatggca ttacgatctg ttggcgggga agtgatcgag caaccgcgta
    10381 acgaaaggga acgttggctg gtcaatactg tagcaaccca ggctcgtcag gcagggatcg
    10441 ctatgccgca agtggctatc taccatgcgc cggacatcaa cgcttttgca accggtgcgc
    10501 gccgtgatgc ctctctggtt gctgtcagca ccggtttgct gcagaacatg agcccggatg
    10561 aagccgaggc ggtaattgct cacgaaatca gccacatcgc caatggtgat atggtcacca
    10621 tgacgctgat tcagggcgtg gtgaacacct tcgttatctt tatttcccgt attctggcgc
    10681 agcttgccgc gggttttatg ggcggaaatc gtgatgaagg tgaagagagc aacggcaacc
    10741 cgctgatcta ctttgcggtt gcaacggttc tggaactggt gtttggtatt ctggcgagca
    10801 ttatcaccat gtggttctcg cgtcatcgtg aattccatgc tgatgccggt tcggcaaaac
    10861 tggttggtcg cgagaaaatg attgccgcgc tgcagcgcct gaaaaccagc tatgaaccgc
    10921 aagaagcaac cagcatgatg gctttctgca ttaacggtaa gtcgaaatcg ctcagtgagt
    10981 tgttcatgac tcacccgccg ctggataaac gaattgaagc tctgcgtacg ggtgaatacc
    11041 tgaagtaata tgaagtaaat actaaaaagt gcgtcctgat cgacgcgctt tttttatgcc
    11101 ctggatcgtg gctgagtgat acgtaaacca ctgacacagg cagcaatcac tgccagaata
    11161 gccgcagcca tcagcgaaac gtgcgtacca ttatcaccga actgatttag catcagcgcc
    11221 accagtgccg cgccgctact ctgacccagt agacgagcag ttcctaacat gccactggct
    11281 ccaccgctac gttcgcgagg agcggaggta ataatggtgt ggttattggg tgactggaac
    11341 aagccaaatc cagcgccaca taagatcatc ggccagataa tattgatatc cgcaggtgac
    11401 gcgggcagca gaaccaggga aaaaagcccc gcagccatga tgaacaaccc tagagcaccc
    11461 agcaatcctg catgtacgcg ttcaatcaaa tagcctgcca acggagccat caccatcgtt
    11521 gctaacggcc acggtgtcag aagtaaacct gtttcgactt cactacgccc gagcacggtt
    11581 tgcaggtaaa agggcaggga aaccattgcc agcatttgtg cgcagaaaga gcaaacagat
    11641 gtgcaaatag aaagtgaaaa cagcgggata cgcagtaaat ccaccggtag cagcggtacg
    11701 ggaagagaaa gctggcggcg aataaagaaa ataccaacaa caaccattac caccagttcc
    11761 gcagcaatta acgtcagcga ttgcccctga gcgaaaccac tcaacgcagt gataagcagg
    11821 ccgaaggtta acgcgttcat cacggcgctg ggcaggtcga aacggggttt actggcgcga
    11881 gaaccattgg gtggcagaaa acgcatcgcc agaagcaggg cgataatacc taacggtacg
    11941 ttgattaaaa ataaccattt ccaggatgcg atggagagga ttgctgcagc aattgtcggc
    12001 ccggcagcag aagagacggc aacaataaac gagtttatgc ccatccctct ccccagaaaa
    12061 cgttgtggat agatcaggcg gataagtgcg gtattaacgc tcatcaacgc tgcaccgccg
    12121 aaaccttgta tgacacgcgc aagggtgagc atttgcagcg aatcagaaag ggcgcagaac
    12181 agtgaagaca acagaaaaac gaccagaccg catttataaa tacgtcgata gccaaacata
    12241 tcgcccagaa acgaaaacga gagcagggag atgacaatgg cgatttgata ggcgttcact
    12301 acccagatgg aactggctgg cgtggcatga aggtccgtgg cgattgttgg cagggcgacg
    12361 ttggcgattg cgccgtcaag gacagccatc gaaataccaa tcacaatggt taatatcgca
    12421 ccgtatcgct ggggcaatgg caggccgtcg gcctgaactt ttggcatagg aattttatat
    12481 ctttggtgaa taatcaacag gggtatgatt ctagcactga tattcacggt ggatgtcgca
    12541 gaattgtaac taaccggcaa aagatggatt gcgacccccc tggtgcgaaa ttataataaa
    12601 aaacagttct gatttttata aaacactcgc aatgaggtga taaatggcta acgcagatct
    12661 ggataaacag cctgattctg tatcttccgt gctaaaagtt tttggcattt tgcaggcgct
    12721 gggtgaagag cgcgaaatag ggataaccga gctgtcgcag cgcgtcatga tgtcaaaaag
    12781 caccgtttat cgctttttac agaccatgaa aaccttaggt tatgtggcgc aggaagggga
    12841 gtcggagaaa tattccctga ccctgaaatt gtttgaactg ggcgctcgcg cgttacaaaa
    12901 cgtcgattta attcgtagcg cagatatcca gatgcgtgag atctcccgcc tgaccaaaga
    12961 aactatccac ctcggcgcac tggacgaaga cagtattgtt tacattcaca aaattgactc
    13021 tatgtacaat ttgcgcatgt attcacggat tgggcgtcgt aatccgctgt acagcaccgc
    13081 gattggtaag gtactgctgg catggcgcga tcgcgatgaa gtgaagcaaa ttcttgaggg
    13141 cgtggagtat aaacgcagta ccgagcggac catcaccagt acagaagcgt tattacccgt
    13201 tctggaccag gtgcgcgagc aggggtatgg cgaagataac gaagagcagg aagaagggct
    13261 gcgatgcatt gcggtaccgg tatttgatcg ctttggcgtg gtcattgccg gtttgagcat
    13321 ctccttcccg acgttgcgtt tctctgaaga gcgtttacag gaatatgtcg caatgttgca
    13381 taccgcagcg cgcaaaattt ctgcccaaat gggttatcac gactatccgt tctgatgaga
    13441 gtaagaacct gtcggaatat caaacagaca ggttctttat ttagcatgag aaaaataaag
    13501 ttgaaggtgc gttatattaa acgcgcttgc tataagaata ttttactcat gagtgagaat
    13561 ctggttattt attaccctta accattatcg accacgatat tgcttttacg taacagtggg
    13621 caatctgtta tccccaagaa accactttta gtgtgcaagt attgtaccgt gctggtgcct
    13681 ctggcagtca gataggtaca ttgcaaacct aatcctgcgg cattctcttt gcttccaatc
    13741 aaaacgccat atccgctgag taataatcct atccatacca gtgctatcag cataactgtg
    13801 cgaatgatga atcgcattac aacctcttct ctttttatgt tcgcttaatc gtagcggcaa
    13861 tatgcgctga agcaagctac tcattccgaa aaagcacgaa tatcgacata gttaggcgct
    13921 gtttaactaa cgcatgctag tttaatgaca taaggtaggt gaagcggaga ttggagtgaa
    13981 aaagtttcga tgggtcgttc tggttgtcgt ggtgttggct tgcttgctgc tttgggcgca
    14041 ggtattcaac atgatgtgcg atcaggatgt acaatttttc agcggaattt gtgccattaa
    14101 ccagtttatc ccgtggtgat atcatttttt cagcactaga ttttcttcct ctccagcagt
    14161 ggtagaatag cgcctctact cagaggtggt gaaatgaacg aagttgtaaa ttcaggcgtg
    14221 atgaacattg cgtctttggt tgtatcggtg gtggttattc ttatcgggct catcttgtgg
    14281 ttttttatca atcgtgccag ttcacggact aacgaacaga ttgaactgct tgaggcgttg
    14341 ctggatcagc aaaaacgtca aaatgcactg ttacgtcgtt tgtgcgaagc aaacgaacca
    14401 gagaaagcag ataaaaagac cattgagagt caaaaatcgg ttgatgacga agatattatt
    14461 cgcctggtcg ccgaacgata atccttcctc aatcttgagt aaccccgtcg ttgaagggtt
    14521 actcaattca tcctgattat acgcactgtc atggtgcaac tgcttcaaat taaaactctg
    14581 gtctgacgtc aaataattat catattattt atttgcgtaa cgggatatca gaaaaagata
    14641 ttttcctcat taatttatta ggatgtttac atcggatttg tgattaagcg tggtattatt
    14701 tattagcgaa acgtttctct cttgattttt ttgctcattc atcaattttt cttatttta
//
LOCUS       DAJTMK010000096        14754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTMK010000096 DAJTMK010000000
VERSION     DAJTMK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14754
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.097"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1268
                     /gene="cmtA"
                     /locus_tag="OO135_002542"
     CDS             <1..1268
                     /gene="cmtA"
                     /locus_tag="OO135_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR5398346.1"
                     /translation="GWLPNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIG
                     TIGVIVGAEIPMFLGSMIMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLL
                     CLLGFEVIGPAVLIANTFVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYP
                     LGMQQASVNGKSIFFMVASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHEL
                     YFPYVLMKPLTIIAMIAGGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATI
                     AGVTVGTLVSFAITSLILKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAF
                     VCDAGMGSSAMGATTFRKRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVT
                     DKPLILINNYIGDPKLDTLFNQLTAEHKH"
     gene            1283..2560
                     /locus_tag="OO135_002543"
     CDS             1283..2560
                     /locus_tag="OO135_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5398347.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            2557..3522
                     /gene="yggF"
                     /locus_tag="OO135_002544"
     CDS             2557..3522
                     /gene="yggF"
                     /locus_tag="OO135_002544"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5398348.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            3544..4053
                     /gene="fumE"
                     /locus_tag="OO135_002545"
     CDS             3544..4053
                     /gene="fumE"
                     /locus_tag="OO135_002545"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5398349.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            4050..4763
                     /locus_tag="OO135_002546"
     CDS             4050..4763
                     /locus_tag="OO135_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5398350.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALREHLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(4735..5412)
                     /locus_tag="OO135_002547"
     CDS             complement(4735..5412)
                     /locus_tag="OO135_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5398351.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(5406..6083)
                     /locus_tag="OO135_002548"
     CDS             complement(5406..6083)
                     /locus_tag="OO135_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5398352.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHCTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6071..6778)
                     /locus_tag="OO135_002549"
     CDS             complement(6071..6778)
                     /locus_tag="OO135_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5398353.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(6779..7357)
                     /locus_tag="OO135_002550"
     CDS             complement(6779..7357)
                     /locus_tag="OO135_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5398354.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(7380..7811)
                     /locus_tag="OO135_002551"
     CDS             complement(7380..7811)
                     /locus_tag="OO135_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5398355.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGKFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8183..9202
                     /gene="epd"
                     /locus_tag="OO135_002552"
     CDS             8183..9202
                     /gene="epd"
                     /locus_tag="OO135_002552"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5398356.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9252..10415
                     /gene="pgk"
                     /locus_tag="OO135_002553"
     CDS             9252..10415
                     /gene="pgk"
                     /locus_tag="OO135_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5398357.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            10630..11709
                     /gene="fbaA"
                     /locus_tag="OO135_002554"
     CDS             10630..11709
                     /gene="fbaA"
                     /locus_tag="OO135_002554"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5398358.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12067..12927
                     /gene="mscS"
                     /locus_tag="OO135_002555"
     CDS             12067..12927
                     /gene="mscS"
                     /locus_tag="OO135_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5398359.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13066..13701
                     /gene="argO"
                     /locus_tag="OO135_002556"
     CDS             13066..13701
                     /gene="argO"
                     /locus_tag="OO135_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5398360.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            13794..14534
                     /locus_tag="OO135_002557"
     CDS             13794..14534
                     /locus_tag="OO135_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5398361.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT         3576 a         3586 c         4044 g         3548 t
ORIGIN      
        1 ccggttggct gcctaacgaa catttcgcca aaattgtcgg cccgatgatt acctatttat
       61 tgcccgtgat gattggttct acaggtggtc atctggtcgg cggtaaacgc ggggcggtca
      121 tgggcggaat aggtactatt ggtgtgatcg ttggcgcaga gatcccgatg ttccttggct
      181 caatgatcat ggggccgctc ggtgggttgg tcataaaata tgtcgataag gcactggaaa
      241 aacgcatacc tgccggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga
      301 tgctcctttg tctgctgggt tttgaagtga tcggcccggc ggtgttaatt gccaatactt
      361 tcgtcaaaga gtgtatcgag gcgctggtac atgcgggtta tctgcctctg ctgtcagtca
      421 tcaatgaacc ggcgaaagtg cttttcctta ataatgcgat cgatcagggc gtctattatc
      481 cgctgggaat gcaacaggct tcggttaacg gtaaatccat cttctttatg gtggcctcta
      541 acccaggtcc gggcctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta
      601 aacgttctgc gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt
      661 tcccatatgt gctgatgaag ccgctgacta ttattgccat gattgcgggc ggtatgtctg
      721 gtacctggat gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta
      781 tctttgctta cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta
      841 cggtaggtac cctggtgtcc tttgctatta cttcgctgat actgaagatg gaaaaaacgg
      901 tggaaacgga gagcgaagat gaatttgctc agtcagccaa tgcggttaag gcgatgaaac
      961 aagagggtgc attctcgtta agcagggtta agcgtatcgc ctttgtttgc gatgcgggga
     1021 tgggctccag tgcgatgggc gcgaccacct tccgtaaacg cctggaaaaa gcggggctgg
     1081 caatagaagt aaaacattac gcgatagaaa acgtgcctgc ggatgccgat atcgtcgtca
     1141 ctcacgccag tctggaaggg cgcgtgaaac gtgtgacgga taaaccgctg atattgatta
     1201 ataactatat tggcgatcca aaactcgaca ctttatttaa tcaattaacc gccgaacata
     1261 aacactgatt acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga
     1321 tgtccgtctg cgcgaatttg aactgccaga aattaccgat aatgaactgt tagtgagtgt
     1381 aatttctgac agcgtctgtt tatcgacctg gaaagcggcg ttactcggta gtgaacataa
     1441 acgcgtaccc gacgatttag aaaatcatcc ggtcattacc gggcatgaat gtgccggggt
     1501 tattgtcgaa gtgggtaaaa atctcactgg caaatataaa aaaggccagc gttttgtatt
     1561 gcaaccggcg atggggttac caagcggata ttcagcaggc tacagctacg aatattttgg
     1621 cggtaacgcc acctatatga ttattcccga aatcgccatt aatttgggct gcgtattacc
     1681 gtatcacggc tcttattttg ctgcggcgtc gctggtagag cctatgtgct gcattattgg
     1741 tgcttatcat gccaattatc acaccacgca atatgtttat gagcatcgca tgggcgtcaa
     1801 acctggcggc aatattgcac tgctggcgtg tgcgggtccg atgggcattg gcgctatcga
     1861 ttacgccatt aacggtggca tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa
     1921 acgcctggcg caggtgcaga agctgctgcc ggtggatctg gcggccagta aaggcattga
     1981 gctggtgtat gtgaatacca aagggatgag cgatccggtc cagacgctgc gggcgctgac
     2041 aggagatgtc gggttcgatg acatttttgt ttatgcggcg gtgcctgctg tcgttgagat
     2101 ggctgacgaa ttactggcgg aagatggctg tctgaacttc tttgccgggc cgacggataa
     2161 aaacttcaaa gtgccgttta atttctacaa cgtccattac aacagcacgc acgtagtcgg
     2221 tacatccggc ggttcaacgg acgacatgaa agaggcgatt gcgttaagcg ccacggggca
     2281 gttgcagccg tcctttatgg tcacgcatat cggtgggctg gatgcggtgc cagataccgt
     2341 gctcaatctg ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc
     2401 gctcactgcc attgccgatt ttgccgaaaa aggcaaaacc gatccgctat ttaaagagtt
     2461 ggcgcggctg gttgaggaaa cgcacggcat ctggaatgaa caggccgaga aatatctgct
     2521 ggcacaattt ggcattgata tcggggaggc cgcgcaatga tgtccctggc gtggccatta
     2581 tttcgcgtta cggaacaggc agcgttggct gcctggccgc aaaccggatg tggcgacaaa
     2641 aataaaattg atggcctggc ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt
     2701 cgtggacgag tggttatcgg cgaaggagaa attgaccatg cgccgatgct gtggattggc
     2761 gaagaggtgg gcaaaggcga tgggccagaa gtcgatatcg cggttgatcc cattgaaggc
     2821 acgcggatgg tggcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcgg
     2881 gatagcctgt tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct
     2941 gccggggtga ttgatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg
     3001 ttaggtaaac cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg cctgagtgcc
     3061 gcgattgagg aagcaacaca gctgggcgtg aaggtttttg ccctgccgga tggcgatgtc
     3121 gccgccagcg tgctgacctg ctggcaggat aatccttacg atgtgatgta caccatcggc
     3181 ggtgcgccag aaggcgtgat ttctgcctgt gccgttaaag cattaggcgg ggatatgcag
     3241 gccgagctga ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca
     3301 gagcaggaga gcaagcgttg caaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc
     3361 gatgaactgg tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggcgag
     3421 ttggtgaacg gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc
     3481 ggcgcggacc aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga
     3541 ccaatggcga agctgacaga agatgatgtg cttgagcaac tggatgcaca ggacaattta
     3601 ttttcattta tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg
     3661 tcgctgtttg tcgataacga tgaagagatc gtggaatatg cagtgaagcc gttactcgcc
     3721 caaagcggcc cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa
     3781 atggataaat ggctgtacgc cgatatcacg catttttccc agttctggca ttacctgaac
     3841 gaacaggatg aaacgcccgg atttgccgat gacatgacct gggattttat cagcaacgtc
     3901 aatagcataa cccgcaatgc gatgctctat gacgcgttga aagcgatgaa gttcgccgat
     3961 ttcgccgtct ggtcagaggc gcgttttagc gggatggtca aaacggcgct gacgctggca
     4021 gtaacgacaa tcttaaagga attaacgccg tgaaaattga attaacggtg aatgggctga
     4081 agatacaggc acagtaccag aacgaagaga ttgaaaatgt acataaaccg ttgctgcgta
     4141 tgctggcggc cttacagacg gtaaatccgc agcggcgtac ggtggttttt ctttgcgctc
     4201 cgccggggac gggtaaatct accctaacca ccttctggga atatctcgcg caacaagacc
     4261 cggagctgcc cgctattcag acgctcccga tggatggttt tcaccattac aatagctggc
     4321 tggacgcgca tcaattgcgc cccttcaaag gcgcaccaga gacatttgac gttgcgaaac
     4381 tggcggaaaa tctgcgccag gtggtggaag gggattgtac gtggccgcag tacgatcgac
     4441 aaaagcatga tcctgttgaa gatgcgttgc acgttaccgc accactcgtc atcgtcgaag
     4501 gaaactggtt gttgctggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct
     4561 cgatctttat caacgcgcca gctactgcgt tgcgggagca tctggtgggg cgcaagctgg
     4621 cgggaggggt atcgctggca gatgcagaag ccttttatga ccgtaccgac gggccgaatg
     4681 ttcgccgggt gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg
     4741 gtgaatatcg tctggtggat taacgttcct gattacacca tcttccagtc gcaccacacg
     4801 agagaaatga cgccgggtaa atgcggcgtc atgactgatt gctaccacgc tcgtgcctcg
     4861 ctggcggcac ttctccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg
     4921 actcggctcg tccagcagta atagtggcgg agagagtgct tccaggcagg cgacggcgac
     4981 catccgtcgc tgcgcactat gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg
     5041 acaacattgc agggcagcat gggtacgttg agtgatttca tccgccgggc atttttgcag
     5101 tttcaaacca aatgcgatct cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc
     5161 ctgaaacagt acgccaacct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcctg
     5221 ttgttgcagc ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag
     5281 cgtcgatttc cctgcgccat tgtcaccggt cagcgccaac cattcacctt gtttgagttg
     5341 cagagaaatg tcgcaaagac aatccgttgc cgcgccaggc cagcgataag atatctgatt
     5401 taacgttaac atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtag
     5461 ttcaggaaag gtgtgcaatg acgttcaaaa agaataatca gcgaaccacg ttcaaacgcc
     5521 cagtgttgta agcgctgcaa cagcatttcg ctggcctgcg gcgtcaggcg gctaaaggct
     5581 tcatcaagaa ttaagagctt tggctgcatc gcgatggcgc aggcgatgac cacgcgctgt
     5641 gtctctccac cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcaga
     5701 gtcagggcgg catcaatccg cgccataatc tctgcttcag caagacacag attttctggc
     5761 ccaaaggcaa cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat
     5821 tgctggacga gctggatggt ggcggattgt tcattaagcg ggagatgccc aatgggcgta
     5881 ccgaggagcg tccccgtgcc tgttatctct cctggcagaa agtccggata ccagcccgcc
     5941 atcagctgtg ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc
     6001 ggtttgacat agtgaaagtc ataacagaac ggaggatgcg ttgagtgtgt cgggcaataa
     6061 cggaactgtt ctaacgtaac catatccacg ctccgaattc tgtcagcatc agcaagatca
     6121 tggtgtaacg cgctacgcgt tgtagggtgc tatcggctgg tgcccacaac gttgttcgtc
     6181 ggttgtggat ccgaaatgcc cgcatatcca gcgccgcacc acggacagcc agatcgttaa
     6241 gcgcattgtg tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc
     6301 cctcatccag cggcacgccg cgggcacgct gcgcttcatg aataatcgct aattgccgtt
     6361 ttaactgctc aacgaccaat aacggcccgg caaacagata agcgacgcct ggcggcaggc
     6421 gagaagcaaa cagagcgcga ataaaacatt gcacggggac gtactgcatc cacaactgtg
     6481 aggttgaaac aatcgccaga atccgcaacc agagcgtaat agcatgcgac caccgttctg
     6541 gcgagcgtgg cgtgccgctt agccattctg gtagccagcc accgtgtacc agccacagcc
     6601 cggctcccag cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta
     6661 gcgcaataag gcagaaaaag gtcgccgcgc tatagatcgg caaaatggtc tgcgcgggca
     6721 ggataagcgt ggtgcaggcc gccaatgccc acagtgttaa tgaggtaaat ggatgcattt
     6781 agcgcacggc agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tggcgcagta
     6841 aaagccagac aatcaccgct gtaagaattt tatcgaccag gttcgcgcct atcaccgtaa
     6901 tcgccaccga ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg
     6961 ctccgctacc ggtgacaccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta
     7021 ccgcaagcgt aataatcacg ccgctgacaa cgacttttgg cagggtgcga aaccaacccg
     7081 ctcgcgccag ccaaccggcg accaggccga tgaccatcgc gacgggtgca aatgcggcgg
     7141 cgatcgggtc ggtcagcaat ccccagagta aattggtcag taagccggtc agcattccga
     7201 tcaccggacc aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aatataggta
     7261 atttgaccat gctggccagt tgcccgccga tcatattgat ggcgatagat ataacgatca
     7321 acaccagtgc ctgactggaa aagtggctac gcgccataaa aaaacctctt tgaaagtagt
     7381 tatttaacgg gggaaatatg caactcgtcg taaccagaaa gcgcgcaagg ttggcggcta
     7441 aacgccagtt cctcaaggct accgatcacc agttcaagcg tggtccgcac ggtacagccg
     7501 ggcatcatat gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgc
     7561 tcaccactct gttccagcgt gccgtttagt gagatcactt cgaatttacc gttcaggagc
     7621 gtggtgcctt cttgcctggc atagcgcaag gcgacgtcag tcaggctacc ggtacagcct
     7681 gcaatccagg cggcatgaag ttgatgctgc tgtgcaaaag cgtgtagctg ggaaaaaact
     7741 tcctgcccgg ggaggaggcg cagtgcgtaa aagcgtgcgg aggaagaata tgggtagggc
     7801 aatgtcatca ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat
     7861 cagtcatatt ctgatataag tcagggtttg tgacggcaca acgaaaagat tttgctacgc
     7921 ttattcgtga ttctgcaaag tggaataaat tccacagggg cggggctgaa aacgtgacgt
     7981 aagtcagata attgctttct ttgactggac aatcattcct tttattccac gtttcgctta
     8041 tcctagctga agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt
     8101 gcgacaggta aggtttcccc ggacgatttg ctggattact ctgtctggca aatttgctca
     8161 gggaaaacct tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat
     8221 cgggcgtaat gtggttcgtg ctttgtatga atccggacgc cgggcggaaa ttaccgtggt
     8281 ggcaatcaac gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag
     8341 ccatggccgt tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga
     8401 cgccatccgc gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt
     8461 tgatgtagtc ctcgactgca ccggcgtata tggctcccgc gagcatggcg aagcgcatat
     8521 tgccgccggg gccaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac
     8581 cgttgtttac ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaacgc
     8641 ttcctgtacc acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat
     8701 tgagtccggc actgtgacca caattcactc cgccatgcac gatcaacagg ttattgatgc
     8761 ataccatcct gacctgcgtc gcacccgggc agccagccag tcgatcattc cggtcgatac
     8821 taaactggcc gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaagcgat
     8881 tgcggtacgt gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa
     8941 acctgtaaaa gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca
     9001 tggtatagtt gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca
     9061 cagtgccatt gtcgatggca cccaaacccg ggtcagtggc gcacacctga tcaaaacgtt
     9121 ggtctggtgc gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat
     9181 ggctactgtt gctttcaggt aagacgcaag cagcgtctgc aaaactttta gaatcaacga
     9241 gaggattcac catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat
     9301 ttatccgtgc ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta
     9361 tccgtgcttc tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa
     9421 cttcccacct gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg
     9481 ttgttaacta cctgaaagac aaactgtcta acccggttcg tctggttaaa gattacctcg
     9541 acggcgttga cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag
     9601 gcgagaagaa agacgacgaa accctgtcca aaaaatacgc tgcactgtgt gacgtgttcg
     9661 taatggacgc attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat
     9721 tcgctgacgt tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag
     9781 cactgaaaga acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca
     9841 aactgaccgt tttggactcc ctgtctaaaa tcgctgacca gctgattgtt ggtggtggta
     9901 tcgctaacac ctttatcgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg
     9961 acctggttga cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg
    10021 ttcgcgtagc aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg
    10081 tgaaagctga cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa
    10141 tcctgaagaa tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga
    10201 acttccgcaa aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca
    10261 tcgctggcgg cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct
    10321 cctacatctc cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg
    10381 cagtagcgat gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc
    10441 ccttgtttca gcgcgctttt agagcacgca ttttttcata ctctaaataa ttcgagttgc
    10501 aggaaggcga caagagagtg aatccccagg agcttacata agtaagtgac tggggtgagc
    10561 gaacgcagac gcagcacatg caacttgaag tatgatgagt ataaggcccg acgatacagg
    10621 acaagagaca tgtctaagat ttttgatttc gtaaaacctg gcgtaatcac tggtgatgac
    10681 gtacagaaag ttttccaggt agcaaaagaa aacaacttcg cactgccagc agtaaactgc
    10741 gtcggtactg actccatcaa cgccgtactg gaaactgctg ctaaagttaa agcgccggtt
    10801 atcgttcagt tctccaacgg tggtgcttcc tttatcgctg gtaaaggcgt gaaatctgac
    10861 gttccgcagg gtgctgctat cctgggcgcg atctctggtg cgcatcacgt tcaccagatg
    10921 gctgaacatt atggtgttcc ggttatcctg cacactgacc actgcgcgaa gaaactgctg
    10981 ccgtggatcg acggtctgct ggacgcgggt gaaaaacact tcgcagctac cggtaagccg
    11041 ctgttctctt ctcacatgat cgacctgtct gaagaatctc tgcaagagaa catcgaaatc
    11101 tgctctaaat acctggagcg tatgtccaaa atcggcatga ctctggaaat cgaactgggt
    11161 tgcaccggtg gtgaagaaga cggcgtggac aacagccaca tggacgcttc tgcactgtac
    11221 acccagccgg aagacgttga ttacgcatac accgagctga gcaaaatcag cccgcgtttc
    11281 accatcgctg cgtccttcgg taacgtacac ggtgtttaca agccgggtaa cgtggttctg
    11341 actccgacca tcctgcgtga ttctcaggaa tatgtttcta agaaacacaa cctgccgcac
    11401 aacagcctga gcttcgtatt ccacggtggt tccggttcta ctgctcagga aatcaaagac
    11461 tccgtaagct acggcgtagt gaaaatgaac atcgataccg atacccaatg ggcaacctgg
    11521 gaaggcgttc tgaactacta caaagcgaac gaagcttatc tgcagggtca gctgggtaac
    11581 ccgaaaggcg aagatcagcc gaacaagaaa tactacgatc cgcgcgtatg gctgcgtgcc
    11641 ggtcagactt cgatgatcgc tcgtctggaa aaagcattcc aggaactgaa cgcgatcgac
    11701 gttctgtaag atattccttt ctgcttatct caaggcccgc tctgcgggtc tttttttcgc
    11761 caaaagcaaa attgcctgat gcgctacgct tatcaggcct acacgtatcc tgcaatatat
    11821 tgaatttgca ggtttttgta ggccggataa ggcgttcacg ccgcatccgg catgacaaag
    11881 cgcacattga tatctgtctg agccccgctc tgcggggatt ttttcgccaa aagcaaggtg
    11941 attcagatga gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc
    12001 agactgcccg tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa
    12061 tattgaatgg aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta
    12121 gctaaccagg cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc
    12181 atcgttggtt tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc
    12241 cgtaaaatcg atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc
    12301 gcctttacgc taatcgctgc cctgggacgc gtgggtgtac aaacagcgtc agtcattgct
    12361 gtactcggtg ccgcaggctt agctgttggt ctggctttgc aggggtcact ttctaacctg
    12421 gccgctggcg tgttacttgt catgttccga ccgttccgtg ccggagaata tgttgacctc
    12481 ggcggcgtag ccggtactgt gctgagtgtg cagattttct ccaccaccat gcgtactgca
    12541 gacggtaaaa ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc
    12601 tcccgcgagc cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc
    12661 gatcaggtta agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat
    12721 cgcgaaatga ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc
    12781 gtctggagca acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa
    12841 cgtgaatttg atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag
    12901 cgggtgaaag aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct
    12961 ctctcttatt agttattctg attgccaatt aatattatca atttccgcta ataacaatcc
    13021 cgcgatatag tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac
    13081 tttcaaggtc ttgctcttgg ggcagctatg attctgccgc tcggcccaca aaatgctttt
    13141 gtgatgaatc agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc
    13201 agcgatttgg tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg
    13261 ccgtggttgc tggcgctggt cacctggggc ggcgtagtct tcttgctctg gtatggtttt
    13321 ggcgctttta aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag
    13381 caaggcagat ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt
    13441 tacctggata cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa
    13501 cgttggtttg cgctcgggac aattagcgcc tctttcctgt ggttctttgg tctggctatt
    13561 ctcgcagcct ggctggcacc gcgcctgcgc acggcaaaat cacagcgcat tatcaatctg
    13621 gtagtgggat gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat
    13681 gcacaagcct tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct
    13741 tgcctccaga ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt
    13801 tcaaagttat cgccctagcg gcattaatgg gtattagcgg gatggcagcg caggctaacg
    13861 aattgccgga tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag
    13921 acattgccac tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga
    13981 aacaggcaga tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga
    14041 aaaaagatat cagctcagcg aatttacgca cccagccaga ttatgattat caggatggta
    14101 aaagtatcct gaaaggctac cgcgctgtga gaacggtgga agtcacgctc cgtcagttag
    14161 acaaactgaa ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttcag
    14221 tgtcgctggg cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg
    14281 ataacgcgat tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg
    14341 tatatagcgt gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga
    14401 aagccgatgc cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg
    14461 atgatcaggt cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac
    14521 ctgcagcaca ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata
    14581 ttgcctcctt tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc
    14641 ggtttcatca aggataggct gactgaaacg gttaagcgga caaggatgcg gggaggtgcc
    14701 cctattaagt tgtcaagcat gttatgaccc ctgcggggta taaaaagtcc cgtc
//
LOCUS       DAJTMK010000097        14736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTMK010000097 DAJTMK010000000
VERSION     DAJTMK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.098"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO135_002558"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO135_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5398362.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO135_002559"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO135_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5398363.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO135_002560"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO135_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5398364.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO135_002561"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO135_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5398365.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO135_002562"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO135_002562"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5398366.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO135_002563"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO135_002563"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5398367.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO135_002564"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO135_002564"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5398368.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO135_002565"
     CDS             complement(4598..5320)
                     /locus_tag="OO135_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5398369.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO135_002566"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO135_002566"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5398370.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO135_002567"
     CDS             6520..6738
                     /locus_tag="OO135_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5398371.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO135_002568"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO135_002568"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5398372.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO135_002569"
     CDS             complement(7472..7672)
                     /locus_tag="OO135_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5398373.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OO135_002570"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="OO135_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5398374.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OO135_002571"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="OO135_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5398375.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPCEQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="OO135_002572"
     CDS             10169..12082
                     /locus_tag="OO135_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5398376.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYADRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSNVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="OO135_002573"
     CDS             12082..13104
                     /locus_tag="OO135_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5398377.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="OO135_002574"
     CDS             13098..13316
                     /locus_tag="OO135_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5398378.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIIPRSQFRE"
     gene            13370..14239
                     /locus_tag="OO135_002575"
     CDS             13370..14239
                     /locus_tag="OO135_002575"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5398379.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="OO135_002576"
     CDS             complement(14294..14698)
                     /locus_tag="OO135_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5398380.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3643 a         4026 c         3612 g         3455 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctag tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aataatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa taacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggttcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca caggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgcta gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttatcgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcaaggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caagcccaga gccgcattaa gatgcttgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatgccgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcaa cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg
    12301 cgtttagtga tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta ttccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagta
//
LOCUS       DAJTMK010000098        14417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTMK010000098 DAJTMK010000000
VERSION     DAJTMK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14417
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.099"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            160..603
                     /locus_tag="OO135_002577"
     CDS             160..603
                     /locus_tag="OO135_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5398381.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRITLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            839..1657
                     /gene="ybhA"
                     /locus_tag="OO135_002578"
     CDS             839..1657
                     /gene="ybhA"
                     /locus_tag="OO135_002578"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5398382.1"
                     /translation="MTTRVIALDFDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1658..2716)
                     /gene="modC"
                     /locus_tag="OO135_002579"
     CDS             complement(1658..2716)
                     /gene="modC"
                     /locus_tag="OO135_002579"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5398383.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSIMKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQEEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2719..3408)
                     /gene="modB"
                     /locus_tag="OO135_002580"
     CDS             complement(2719..3408)
                     /gene="modB"
                     /locus_tag="OO135_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5398384.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3408..4181)
                     /gene="modA"
                     /locus_tag="OO135_002581"
     CDS             complement(3408..4181)
                     /gene="modA"
                     /locus_tag="OO135_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5398385.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGHPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4347..4496)
                     /gene="acrZ"
                     /locus_tag="OO135_002582"
     CDS             complement(4347..4496)
                     /gene="acrZ"
                     /locus_tag="OO135_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5398386.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4625..5413
                     /gene="modE"
                     /locus_tag="OO135_002583"
     CDS             4625..5413
                     /gene="modE"
                     /locus_tag="OO135_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5398387.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5481..6953
                     /gene="modF"
                     /locus_tag="OO135_002584"
     CDS             5481..6953
                     /gene="modF"
                     /locus_tag="OO135_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5398388.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLVSLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7214..8230
                     /gene="galE"
                     /locus_tag="OO135_002585"
     CDS             7214..8230
                     /gene="galE"
                     /locus_tag="OO135_002585"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5398389.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8240..9286
                     /gene="galT"
                     /locus_tag="OO135_002586"
     CDS             8240..9286
                     /gene="galT"
                     /locus_tag="OO135_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5398390.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDQDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9290..10438
                     /gene="galK"
                     /locus_tag="OO135_002587"
     CDS             9290..10438
                     /gene="galK"
                     /locus_tag="OO135_002587"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5398391.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10432..11472
                     /gene="galM"
                     /locus_tag="OO135_002588"
     CDS             10432..11472
                     /gene="galM"
                     /locus_tag="OO135_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5398392.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11674..12426
                     /gene="gpmA"
                     /locus_tag="OO135_002589"
     CDS             11674..12426
                     /gene="gpmA"
                     /locus_tag="OO135_002589"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5398393.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12584..13636)
                     /gene="aroG"
                     /locus_tag="OO135_002590"
     CDS             complement(12584..13636)
                     /gene="aroG"
                     /locus_tag="OO135_002590"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5398394.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13952..14332
                     /locus_tag="OO135_002591"
     CDS             13952..14332
                     /locus_tag="OO135_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5398395.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
BASE COUNT         3674 a         3751 c         3640 g         3352 t
ORIGIN      
        1 atttttaaaa cagctatagc ttctggtctt gccgaagaag atccagcttc tgcaaccagg
       61 ccattcagaa ctgaagtgaa gcgcagtcga ctgttaattg acgagtatct gttaatcagg
      121 aaaatagcag acgtccagaa cgaatggttc ggcctttgca tggatcttgc actggtaaca
      181 ggacagcgcg aaggagatct ggcggctatg agatgggagg atataagaga tggcaggcta
      241 tacgtggaac aacaaaaaac aggggcaaaa attcgcatta ctctccccac cacgatttcc
      301 agattgaatt taacacttac cgatgtactg gataacttga aaaaaataaa cggtaaaaac
      361 gaaaagttac tcggagggaa gacagccagg acaatcgcag cacagttcag gattgcccga
      421 gacacatccg gattgaaatg ggaaggtgac ccaccgccat ttcatgaaat ccgaagttta
      481 tccggtcgcc tgcactcagc agaaaaaggc agtgatttca ctcaggcgct ccttggacat
      541 cgttcatcca gcatgacgga taaataccgt gacggacgag ggagggaatg gaaagacatt
      601 tagaattaat ttcgaatgat ttcgaaacac tacttgtaat taattgatta taaaggatta
      661 attcctaaga ggcatttgct tcatgaatgc tcctttgcat ttagcaactg actgtaacag
      721 ctaaaattag tcgcttttgg cggtgaatgc tgaattaaaa cgcagaattt tgtccgatag
      781 tggtatcggt gtaccattcg cgcagacgaa aattcttaac cttaatctgg aatacgccat
      841 gaccacacgc gtgattgctc tcgactttga cggcacctta ttgaccccga aaaagaccct
      901 gcttccttca tcgatagaag ccctggcccg cgctcgcgaa gcaggctatc aattaatcat
      961 cgtcacaggt cgccatcacg tcgctattca tcctttttat caggcgctgg cgctggatac
     1021 acctgctatt tgctgtaatg gcacctattt gtatgattat catgcaaaaa ccgtgctgga
     1081 agcggaccca atgcccgtta ataaagccct gcaactcatt gagatgctga atgaacacca
     1141 cattcacggt ctgatgtatg tggatgatgc aatggtctat gagcacccga ccgggcatgt
     1201 cattcgcacg tctaactggg cgcaaaccct gccgccggaa cagcgtccga ctttcacaca
     1261 agtcgcttct ctggctgaaa cggcgcaaca agttaacgcc gtatggaagt tcgccctgac
     1321 gcacgatgac atgccgcaat tgcagcattt tggtaagcat gtcgaacatg aactgggact
     1381 ggagtgtgaa tggtcctggc acgatcaggt tgatattgca cgcggcggca acagcaaagg
     1441 taaacgtttg acgaaatggg ttgaggcgca aggctggtcg atggaaaacg tcgtggcgtt
     1501 cggcgataac tttaatgata tcagtatgct ggaggccgct ggtacaggcg tggcgatggg
     1561 caacgccgat gacgcggtaa aagcgcgcgc caacattgtg attggtgata acaccaccga
     1621 cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc gacacacttt
     1681 taatttgcgc gtacagccac aggcctggtt tgatcgccag ttcatccctg gcccacgggc
     1741 tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttcctcc tgaccgttgt
     1801 cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt tgctgcggcg
     1861 gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca gcttgcagcg
     1921 gttcgtccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt
     1981 aatgcggatg atgctcaagc accgtcactt tcataatgct actttgttgc tctttcggca
     2041 gccacggatt catcacgcta ctaccccaga cttcttccag tgcgccaaag gctttcacct
     2101 gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc agcgaatggc
     2161 tgacatacaa catcgggatg ttgatttccc gcgtcagacg ttgcagataa ggcaacagtt
     2221 cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc aattctggtg
     2281 ccgtcagcaa agcccgccca atcgctacgc gctgtttttc gcctccggac aggctgcctg
     2341 gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg aactgatcga
     2401 ccatgctttt cgccatgccg tagcgcagat tgccacgcac tttgtaatgc ggaaacagcc
     2461 gcgcatcctg aaaaacatag ccaacgcgac gcttttctgg cgtcaggcag ataccttttt
     2521 cggcatcatt tagtacccga ccattgagga caatcctccc tttttgcggg cgcgtcagtc
     2581 cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag atagcagtga
     2641 tgccattggc gggcagcgtt tcattaatag tcaggcaatg gttgcccaac gtctgggaaa
     2701 aattcagttc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg ccagccattc
     2761 tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc
     2821 actttccccg ccgggggtct ggatcagggt atacatggca gagggaatgg ttcgcgtttc
     2881 gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa
     2941 agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga tagtaaagaa
     3001 aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tcgagtttga catcaacccc
     3061 ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg cggcagcgag
     3121 aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca gacgttcacc
     3181 gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca cgggcggtaa
     3241 caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg tgcaacgcac
     3301 cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca gggaagaaac
     3361 tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta cttggttgta
     3421 aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc ataaaatgct
     3481 ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac ttttttatgt
     3541 gaatcttccg ggaaggtggc aaccactttt acccctttgc tgacaactgc gtcagaaccg
     3601 taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg ccagcgcgcc acgaacatct
     3661 tccgccggag ccaatttcgg ggagagcgta tcccatgcgc ccagtttttg cagtgcttct
     3721 tttgcataaa tgccagcagg gacatgttcc ggatggccaa ccgcgaggcg accgccattc
     3781 agcagcgaag tccagttagt tgtgctgtcg atgatgaaat ctttctgctc gctggctttc
     3841 ggtgctacaa cgaccaggct attgccgagc agtgtctggc gcgaagccgt atcgatcgct
     3901 tttttatcaa ccgcataatc catccatttc tgatcggcag agataaacag atccgcaggc
     3961 gctcccgctt caatctgacg ggccagagta gatgacgaag cgaaagaaga aaccacatcc
     4021 acgccttttt cttttttata ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg
     4081 gcggcgaaca ccgtgatttt cccttcatct gccagtgtat tgccagcaac agcgaaagag
     4141 agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa
     4201 cgttatatag gtgacaatat aacggctagc tcagaatttt cccagcagtt attgctaacc
     4261 tttgtgaggt agataagaaa aaatatcggc tggaaaagca ggaagttgag aaaaagaaat
     4321 gcccgactaa gtgggcattc agggaatcaa tgattttgtc cgggctggtc ttttttacca
     4381 acaccagaaa agatgttgaa tacttcacca agaccgtaaa tcagacccag gatgatggcc
     4441 atcacgacag gtaccatgat tacggcgaat accagacttt ttaataactc taacatggtc
     4501 aactccagat atagtcatga gactattcta accgctaagc acagaaaagc actccccttt
     4561 tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa taagactttt gccaggacat
     4621 tgttatgcag gccgaaatcc ttctcaccct taagctccaa caaaaattat tcgccgaccc
     4681 gcgccggatt tcgctactaa aacacattgc gctttccggt tccattagcc agggagcgaa
     4741 agatgccggt atcagctata aaagcgcctg ggatgccatt aacgagatga atcagttaag
     4801 cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac
     4861 ccgctatggt cagcgactga ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc
     4921 ctttgatgtg ttaagtgacg atgacgccct gccgctgaac agcctgctgg ccgcgatctc
     4981 acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc ggtaccatca ccgcccgcga
     5041 tcatgatgac gttcaacagc atgttgatgt cttactggct gacggaaaaa cacgcctgaa
     5101 agtcgcaatt accgcacaaa gcggcgcacg tctggggctg gatgaaggca aagaagtgtt
     5161 gatattgcta aaagcgccgt gggtaggcat tactcaggac gaagcggtcg cacaaaacgc
     5221 caacaaccaa ttaccgggta ttattagtca tattgagcgc ggcgcagagc agtgcgaagt
     5281 attaatggcg ctacccgacg ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac
     5341 ttctcttcag caagggcaga atgtcacggc ctactttaat gctgacagcg tgattatcgc
     5401 cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac gtatccctgt cagtaatcgc
     5461 tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta
     5521 gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt
     5581 ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc
     5641 cgcttttgaa aggtgaacgg caaagccagt tttcccacat cactcgtctc tccttcgagc
     5701 aattgcaaaa actcgtcagc gacgaatggc agcggaataa caccgatatg ctcggccctg
     5761 gcgaagatga caccggacgc actacggctg agatcattca ggatgaagta aaggatgcac
     5821 cgcgttgcat gcaactggcg cagcagttcg gtattaccgc cctcctcgac cgacgcttta
     5881 aatacctttc cactggcgag acgcgaaaaa ccctgctgtg tcaggcgctg atgtcggagc
     5941 ctgacttgtt gattcttgat gagccgttcg atggcctgga tgttgcctca cgtcagcagc
     6001 tggctgagcg actcgtctcg ttacatcagt ccggtattac tctggtactg gtgctcaatc
     6061 gcttcgatga gatcccggag tttgtccagt ttgctggcgt gctggcggat tgcacgttag
     6121 cggaaactgg cgctaaagag gaactgctcc aacaagcact cgtcgcgcaa ctggcacata
     6181 gtgaacagct tgaaggtgtg caactgccgg agccggatga accttcagca cgtcacgcct
     6241 tacccgccaa cgaaccgcgc attgtgctga acaatggcgt ggtttcttat aacgatcgcc
     6301 ccattcttaa taaccttagc tggcaggtga atccaggtga acactggcaa attgtcgggc
     6361 caaatggtgc aggaaaatcg acgttattaa gcctggttac tggcgatcat ccgcaaggtt
     6421 acagcaacga tttgacgctt ttcggacgac gtcgcggcag cggcgaaacc atctgggata
     6481 tcaaaaagca tatcggttac gtcagcagta gtttgcatct ggattaccgg gtcagcacta
     6541 ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat tggcatttat caggccgttt
     6601 cggatcgcca gcaaaaactg gtgcagcagt ggctggatat tctcggcatt gataaacgta
     6661 cggctgacgc tccgttccat agtctttcct ggggacagca gcatctggcg ctgatcgtcc
     6721 gcgcactggt gaaacatccg acgttgctta ttctcgatga accactacag gggcttgatc
     6781 cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc
     6841 aattgttgtt tgtttcgcac cacgctgaag atgcgcctgc ctgtattacc catcgccttg
     6901 agttcgtgcc ggacggtgga ctctatcgct atgtgctgac aaaaatatat tgagtcggta
     6961 gtgctgacct tgccggaggc ggcctaagcg ccctctccgg ccaacggttc gacgcatgca
     7021 ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc attcataaac cctctgtttt
     7081 ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt
     7141 tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct
     7201 aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg gaagtcatac
     7261 ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag
     7321 taagcgcagc gtactgcctg ttatcgagcg tttaggcggc aaacatccaa cgtttgttga
     7381 aggcgatatt cgtaacgaag cgttgatgac cgagatcctg cacgatcacg ctatcgacac
     7441 cgtgatccac ttcgccgggc tgaaagccgt tggcgaatcg gtacaaaaac cgctggaata
     7501 ttacgacaac aatgtcaacg gtactctgcg cctgattagc gccatgcgcg ccgctaacgt
     7561 caaaaacttt atttttagct cctccgccac cgtttatggc gatcagccca aaattccata
     7621 cgttgaaagc ttcccgaccg gcacaccgca aagcccttac ggcaaaagca agctgatggt
     7681 ggaacagatc ctcaccgatc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg
     7741 ctacttcaac ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat
     7801 tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc
     7861 gatttttggt aacgattatc cgaccgaaga tggtactggc gtacgcgatt acatccacgt
     7921 aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt
     7981 acacatctac aacctcggcg ctggcatagg cagcagcgtg ctggacgtgg ttaatgcctt
     8041 cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg agggcgacct
     8101 tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg
     8161 cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata
     8221 tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta
     8281 caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca
     8341 gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatcaag attgcttcct
     8401 ctgcgcaggt aatgtgcggg tgacaggcga taaaaaccac gattacaccg ggacttacgt
     8461 tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga
     8521 tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga
     8581 tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg tcaaaacctg
     8641 gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtgcaggtct ttgaaaacaa
     8701 aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg caaatagctt
     8761 cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa
     8821 atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt
     8881 cgaaaccgaa cactggttag ccgttgtgcc ttactgggct gcctggccgt tcgaaacgct
     8941 actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc agcgcagcga
     9001 tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt
     9061 cccctactct atgggctggc acggcgcacc atttaatggc gaagagaatc aacactggca
     9121 gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta aatttatggt
     9181 tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg
     9241 tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
     9301 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
     9361 ggcgcctggc cgcgtgaatt tgattggtga acacacggac tacaacgacg gtttcgttct
     9421 gccctgcgcg attgattatc aaaccgtgat cagctgtgca ccacgcgatg accgtaaagt
     9481 tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctca atgcgcccat
     9541 tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca
     9601 actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg tgccgcaggg
     9661 tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
     9721 ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
     9781 gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga
     9841 tcatgccttg ctgattgact gtcgttcact ggggaccaaa gcagtttcca tgccgaaagg
     9901 tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
     9961 cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ccctgcgcga
    10021 tgttaccatt gaagagttca acgctgttgc acatgagctg gacccaatcg tggcgaaacg
    10081 cgtgcggcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca
    10141 gggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
    10201 tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag ctgtgattgg
    10261 cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgttgat
    10321 cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac
    10381 aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
    10441 gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac
    10501 gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttcagc ccgtattccg
    10561 ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga atgctatcag
    10621 gatcaggccg cgtttctggg ggcctctatt ggccgttatg ccaaccgcat cgccaatagc
    10681 cgttatacct ttgacggtga aaccgtgacc ctttcgccaa gtcagggcgt taaccagctg
    10741 cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt
    10801 caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg taatctcggc
    10861 gcaacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca
    10921 gttgataaac cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag
    10981 cagtctgacg tgcgcaatca caagttgcag attctggcgg acgaatatct gccggttgat
    11041 gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacgtcttt tgatttccgc
    11101 agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt
    11161 tacgatcacg cattcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc
    11221 tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctccggc tctgcaattc
    11281 tactccggca acttcctcgg cggcacaccg tcgcggggaa cagaacctta cgccgactgg
    11341 caagggctgg cgttggaaag cgagtttctg ccggacagcc cgaaccaccc tgaatggccg
    11401 caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga atatcagttt
    11461 attgctgagt aatgttaagc cctcgtcatg agggctttat ctcatattgt tcaaatcacc
    11521 agcaaacacc gacatatttg caactcaata ttcacaacaa ccttacactg cgccactatt
    11581 ttcgctatgg ttatgcgtaa gcattgctgt tgcttcgtcg cggcaatata atgagaatta
    11641 ttatcattaa aagatgattt gaggagtaag tatatggctg taactaagct ggttctggtt
    11701 cgtcatggcg aaagtcagtg gaacaaagaa aaccgtttca ccggttggta cgacgtggat
    11761 ctgtctgaga aaggcgtaag cgaagcaaaa gcagcaggta agctgctgaa agaggaaggt
    11821 tacagctttg actttgctta cacttctgtg ctgaaacgcg ctatccatac cctgtggaat
    11881 gtgctggacg aactggatca ggcatggctg cccgttgaga aatcctggaa actgaacgaa
    11941 cgtcactacg gtgcgttgca gggtctgaac aaagcggaaa ctgctgaaaa gtatggcgac
    12001 gagcaggtga aacagtggcg tcgtggtttt gcagtgactc cgccggaact gactaaagat
    12061 gatgagcgtt atccgggtca cgatccgcgt tacgcgaaac tgagcgagaa agaactgccg
    12121 ctgacggaaa gcctggcgct gaccattgac cgcgtgatcc cttactggaa tgaaactatt
    12181 ctaccgcgta tgaagagcgg tgagcgcgta atcatcgccg cacacggtaa ctccctgcgt
    12241 gcgctggtga aatatcttga taacatgagc gaagaagaga ttcttgagct gaatatcccg
    12301 actggcgtgc cgctggtgta tgagttcgac gagaatttca aaccgctgaa acgctattat
    12361 ctgggtaatg ctgacgagat cgcagcgaaa gcagcggcgg ttgcaaacca gggtaaagcg
    12421 aagtaaacgt cattcgttta aaatgagaaa gccgactgca agtgagtcgg cttttttgtt
    12481 gctaacaatg gagcacattg cctgatgcga cgctgcgcga cttatcaggc ctgtggtgat
    12541 tcatcggata cgccactctg acggcgcatc cgacaattaa accttacccg cgacgcgctt
    12601 ttactgcatt cgccagttga cgtaacagag catcggtatc ttcccagccg atgcaggcat
    12661 cggtgatgct cttaccgtag gccagcggct ccccgctctc gaggctctga ttgccttcca
    12721 ccagatggct ttccaccatc acgccaataa tggccttttc gccaccggca atctgctggc
    12781 aaacgtcagc acaaacatcc atctgctttt tgaattgttt ggacgagtta gcatggctga
    12841 aatcgatcat cacctgtgct ggcaggcctg ctttgttcag cccttctttc acttcagcaa
    12901 cgtgcttcgc gctgtagtta ggctctttac cgccgcgcag aatgatatgg caatcgccgt
    12961 taccgctggt attcacaatc gccgaatgcc cccatttcgt tacggacagg aagcagtgcg
    13021 gcgcaccggc ggcattaatg gcatcgatag ccactttaat cgtaccgtcg gtgccatttt
    13081 tgaagccgac tggacaagaa agccctgatg ccagttcgcg gtgcacctgc gattcggtgg
    13141 tacgtgcgcc aattgcgccc cagctcatca ggtcagcgag atattgtggg gtgatcatat
    13201 cgagaaactc acccgccgct ggcagaccgc tgtcgttaat atcaagcagc aatttacggg
    13261 ctatacgaag accgtcgttg atctggaagc tgttatccat atgcggatcg ttaatcagcc
    13321 ctttccagcc caccgtggta cgcggctttt caaaatagac gcgcattacg atttccagct
    13381 catctttcag ctcttcacgc agcgccagca agcgagtggc atactcttta gccgcgacag
    13441 gatcatgaat tgagcacggg ccaatcacca ccaacaggcg atcatcatta cctttcagga
    13501 tcttatggat cgcttttcgg gcatgggcaa ccgtattcgc ggcattttca gtagcgggga
    13561 atttttccag caatgcgaca ggaggaagta actctttgat ttctttgatg cgtaaatcgt
    13621 cgttctgata attcatgtct gttccagtgt tgccatactt atcttagtga atgcaatact
    13681 tccaatctat atcttccgtc agaatgtgta aacggggttt tacattatga acggattaat
    13741 cctggaatta gcaagaaaaa cgccagattg tcgcgaaaaa cgagatctct cctacaattt
    13801 ctaactgtaa ctcctttgtt tgttaattat ttcaagattc tctgctgcgt ttcataaact
    13861 ggctgaaatc ttaaaccaat gccttatatt cacctgcaaa tgcactgttg gaagaggtta
    13921 tccgacataa cgaccataac aggagcatcc tatgaaaatg acaaaactgg ccacattatt
    13981 tctgactgcc actctaagcc ttgccagcgg tgccgcactg gctgccgata gcggagcgca
    14041 aactaataac ggccaggcaa acgccgcaac tgatgcgggc caggtagccc ctgacgcccg
    14101 tgaaaatgtc gcgccaaata acgtcgacaa taacggggta aataccggtt ctggcggcac
    14161 aatgctgcat tcggatggtt cttcaatgaa caatgacgga atgaccaaag atgaagagca
    14221 caagaacacc atgtgcaaag atggtcgatg tccggatatc aataaaaagg tacaaaccgg
    14281 tgatggcatc aataatgatg tcgataccaa aaccgacggt accacgcagt aaccctgcaa
    14341 aatagccaat aatgggagag cggagtctct cccttttaat ttttcacttc taaaagcagt
    14401 atgatgtcat aaagtta
//
LOCUS       DAJTMK010000099        14393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTMK010000099 DAJTMK010000000
VERSION     DAJTMK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.100"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..126)
                     /locus_tag="OO135_002592"
     CDS             complement(<1..126)
                     /locus_tag="OO135_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR5398396.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRD"
     gene            complement(139..1428)
                     /gene="arsB"
                     /locus_tag="OO135_002593"
     CDS             complement(139..1428)
                     /gene="arsB"
                     /locus_tag="OO135_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5398397.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1483..1836)
                     /gene="arsR"
                     /locus_tag="OO135_002594"
     CDS             complement(1483..1836)
                     /gene="arsR"
                     /locus_tag="OO135_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5398398.1"
                     /translation="MSFLLPIQLFKILDDETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            2575..2658
                     /locus_tag="OO135_002595"
     CDS             2575..2658
                     /locus_tag="OO135_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5398399.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(2712..4064)
                     /gene="gorA"
                     /locus_tag="OO135_002596"
     CDS             complement(2712..4064)
                     /gene="gorA"
                     /locus_tag="OO135_002596"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5398400.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(4136..4978)
                     /gene="rlmJ"
                     /locus_tag="OO135_002597"
     CDS             complement(4136..4978)
                     /gene="rlmJ"
                     /locus_tag="OO135_002597"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5398401.1"
                     /translation="MLSYRHSFHTGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMLHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            5181..7223
                     /gene="prlC"
                     /locus_tag="OO135_002598"
     CDS             5181..7223
                     /gene="prlC"
                     /locus_tag="OO135_002598"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5398402.1"
                     /translation="MMNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            7231..7983
                     /gene="rsmJ"
                     /locus_tag="OO135_002599"
     CDS             7231..7983
                     /gene="rsmJ"
                     /locus_tag="OO135_002599"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5398403.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTTLTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(8032..9501)
                     /gene="dtpB"
                     /locus_tag="OO135_002600"
     CDS             complement(8032..9501)
                     /gene="dtpB"
                     /locus_tag="OO135_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR5398404.1"
                     /translation="MNTTTPMGMLQQPRPFFMTFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(9819..10253)
                     /gene="uspA"
                     /locus_tag="OO135_002601"
     CDS             complement(9819..10253)
                     /gene="uspA"
                     /locus_tag="OO135_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5398405.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            10644..10979
                     /gene="uspB"
                     /locus_tag="OO135_002602"
     CDS             10644..10979
                     /gene="uspB"
                     /locus_tag="OO135_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5398406.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(11223..12722)
                     /gene="pitA"
                     /locus_tag="OO135_002603"
     CDS             complement(11223..12722)
                     /gene="pitA"
                     /locus_tag="OO135_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5398407.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            12954..14156
                     /locus_tag="OO135_002604"
     CDS             12954..14156
                     /locus_tag="OO135_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5398408.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
BASE COUNT         3608 a         3689 c         3810 g         3286 t
ORIGIN      
        1 atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc cgctgttgcg
       61 gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa tggtaatgtt
      121 gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg cagccagcgt
      181 cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc cccagctgat
      241 agtcatattc ttctgcgaaa gtacgtgcag ccagagtagt gtagccaggc taccaattgg
      301 ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg cttctttgat
      361 aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg tcggcatatt
      421 gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg cggcccacag
      481 gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc cggcattgcg
      541 caggccataa accaccagat acatgccgag cgagaagatg acaatttgcc agggggcacc
      601 gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa ataatatcag
      661 cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga cgaaaaatcc
      721 caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat ctttgatcgc
      781 ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt tgcgaaaata
      841 gagatgtagc atcaccagcg tggcaacaat cgcggcgata tccaccggca ccatcaccga
      901 ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca ccaggttgga
      961 gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca cgaacgccag
     1021 cgtcgtgcct ttactgaatc ctaaagccag cagcatggcg atgacaatcg gcgtcaaaat
     1081 aagcgctgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca ggacaatcca
     1141 ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg ccgcccattc
     1201 aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa acgcagccgt
     1261 cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca cgcccgtaac
     1321 taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt tcggctgcca
     1381 gataaccaat acaatggtca ggacaaagat agcgcctgcc agtaacataa tgcctcctgg
     1441 ataaaacaca tgcgaaaata catatgtgtt tagctaaatt ttttaactgc aaatgttctt
     1501 actgtctgcg gaacagtttt gtcgagccag gttgcggaca attgcctgaa tcttttcctg
     1561 ttcacatcgc caggcttcat caattatttt cgccgcccat gctggaatat gcggcgataa
     1621 gcggtaatga acccatttac cttgcttgcg gtccagcaat agcccgcttt cacgcagcaa
     1681 tgccaggtgg cgggagatct tgggctgcga ctggtcgaga gcagtgcaga gatcgcagac
     1741 gcataactct cccagttcgc tgagcagtaa aacgatgccc agacgggttt catcatcaag
     1801 aattttgaac aattggatgg gtaacagaaa tgacatattg cgctcctgat tgttgcaggt
     1861 agtgtctctc ttcgaagcgg ataagtcaaa acatatatga tttaacgaat atgtaagtgc
     1921 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga ggtctgaatc
     1981 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct
     2041 ctggctggag tgagagagtt cttatctaac agctcaatac tattagccag tggctaacat
     2101 tgcaagaatt agcgatttct tcagctggcg gagggagcac ttcggtatat atcgcagtga
     2161 cgatttatat actttcactg ggtcatcgtc atattaagcc ttgtccgaat ccggatgtaa
     2221 aaaaaccgac tttgcgtcgg ttttttactt tccagccctg agttggtggc tctgactgga
     2281 gtgagatagc caccatctaa cgcatcaatg ctaataccat actgaaacta ttgcaaggac
     2341 gtgctggttt tataacctgc atgtactgta tgattatcca gttagctctg aggcattttc
     2401 actctggcaa tgcgcataaa cgctttcaaa gtcctggtca gaagtacggg tggtgccgtt
     2461 aactgatgct ctggccggag tgagagagtt cttatctaac aatgagacat gcgccgtgac
     2521 aggcagtgga tgagtaagcg gatgcattct cactccatcg catggagaaa acgggtgatt
     2581 gataaagcaa tcatcgttct aggggcgtta attgcgctgc tggaactgat ccgctttctg
     2641 cttcagcttc tgaactgata gcggaaacgt aattaagggc taagagcaca ctactcttag
     2701 ccctttaaca tttaacgcat tgtcacgaac tcttctgccg ccgttgggtg aatggcgacg
     2761 gtattgtcga agtctttttt ggttgccccc atcttcagcg ccaccgcgaa gccctgcaac
     2821 atttcgtcca taccaaagcc aatgccgtga ataccgacaa tcttctcttc cgatccaacg
     2881 cacaccagct tcatgcggca cggctggcgg tgagtggtga cggcggtata catcgcggtg
     2941 aaagaggatt tatacacttt cacctgatcg tcgccatact gctcgcgcgc ctgcggttcc
     3001 gttaaaccaa cagtaccaat cggcggatgg ctgaagacca cggtcggaat gttgctgtaa
     3061 tccagatgct catccggctt gttattaaac aggcgttcag agagacggcg acccgctgca
     3121 actgccaccg gtgtcagctc cactgcaccc gtgttatcgc ccaccgcgta aataccttca
     3181 atgttggtgt tttgatattt atcgacgacg atatagcctt tttcgttggt tttaacgcca
     3241 gcggcttcga ggttgatgtt gtcattggca ggctcgcgac caatcgccca aatcaggcaa
     3301 tccaccgttt cactgcgacc atcttccagc tccagcgtca ggctaccatc ggcatttttc
     3361 actaccgctt tcgggatggc gttggtgtgc agctgcgggc cttcggcgtt catcacttcg
     3421 accagcgttt cggaaatcat cgggtcgaag ctgcgcagcg gcgcatgttt acgcacaaac
     3481 agatgcgttt tcgcgccgag gccgttaatc acgcccgcca gctcaacggc gatgtaaccc
     3541 gcgccaacaa ccgccacgcg ctctggcaaa gcaggaaggg cgaagaagcc atcagaatca
     3601 ataccgtatt ccacgcccgg aatatccggg tggctcggac gaccgcctgt ggcgatcaga
     3661 atatgatcgg ccgtgatggt ttcgccgttt acctccagca ttttggcatc aacgaagcgg
     3721 gcaaagcctt tgattacatc aacgttattt ttaccgagca cgttttcata ggaagtatga
     3781 atacggtcga tataggcggt acggctggcg atcaacgttt cccagttgaa cttgttgatg
     3841 gtggtatcga aaccataatc agggccgtac atatggatcg cttcacggat ttgcgccgcg
     3901 tgccacatca cttttttcgg cacgcagccg acgttcacgc aggtgccgcc cagctctttg
     3961 gcttcaatca gtgcgcattt ctggccgtac atagccgcgc ggttgatgga ggctataccg
     4021 ccgctgccgc cgccgatggc gatgtaatca taatgtttag tcatgacaga gtgtccttat
     4081 cgttgattac cgcgattgta gcgcgagacg taataggtgc cagcaatggc tgcaattact
     4141 ccggcacgat ccagcttacg gtggcgtgcc cggtgcctgc cggaaccagt ttgctgtgca
     4201 gccacggcag cacgttattc atctgctgtt ccagtttcca cggcgggtta atcacaatca
     4261 tgccggaagc ggtcatgcca cggcgatcgc tgtctggcag taccgccagt tcaatttgca
     4321 gaattttgcg aataccggtc gcttccagat cgtggagcat gcgcttaatt tgctgacgca
     4381 gcaccaccgg ataccacagt gcgtaagtac cagtggcgaa acgtttgtaa ccttctgcta
     4441 tcccgctgac caccgcctga tagtcggttt tcatttcata cggcgggtcg ataaggatta
     4501 aaccacggcg ggaaaccggc ggcagtttgg ccttaagctg ctggaaacca tcggcttttt
     4561 cgacgcgcgc acggctgtct ttctgaaatt cagaacgcag caacgggtaa tcgctcgggt
     4621 gcagttcggt cagttgcagg ctgtcttgtt cgcgcagtag ctggcgagca atcaatggcg
     4681 aaccggggta gtaacgcaac tgaccgctac ggttgaagtg ttttaccaca ttgatgtacg
     4741 cctccagttc tgcgggcaaa tcgtcctgct gccagatacg ggcgatgcct tcgagatatt
     4801 cgccggtacg ctcggcatgt tcgctgccta actgataacg cccggcccct gcgtgggtgt
     4861 cgagatagag aaacggttta tctttctctt tcagcgactc gatgatcagg ctctgaacgg
     4921 tatgtttaag gacgtcggcg tggttgccag tgtgaaagct gtggcgataa ctgagcatgg
     4981 gtaaaggtgt tccggtaagt aaaaatcggc ccgtattcgg ggcgcacaaa agcgtatcag
     5041 gacagtatac cgaaaagagg ccgccgccgc gaaagcgtaa cgtttctcat tgaaattcac
     5101 tacacttaac cccatgctac acacattatg taaagcgcct gttgagcgct tccttaacct
     5161 ctttaaccag gactgcgcga atgatgaatc cgttactgac tccctttgaa ttgcctccgt
     5221 tttctaaaat tctcccggaa catgtcgttc cagccgtgac tcaggcgctg aacgattgcc
     5281 gcgaaaatgt ggagcgcgta gtagcgcaag gggcaccgta cacctgggaa aatctctgcc
     5341 agccgttggc ggaagtggac gatgtgttgg ggcgtatctt ctccccggtc agccacctga
     5401 actcggtgaa aaatagcccg gaactgcgtg aagcctacga gcaaaccctg ccgctgctgt
     5461 cggaatacag cacctgggta gggcaacatg aagggctgta taaagcgtac cgcgacctgc
     5521 gcgatggcga tcattacgcc acgctgaaca cggcgcagaa gaaagcggtt gataacgccc
     5581 tgcgtgattt tgaactctct ggcataggtc tgccgaaaga gaaacagcag cgttacggcg
     5641 aaattgcgac ccgtctttct gaactgggca accagtacag caacaacgtc ctcgatgcga
     5701 caatgggctg gaccaaactc gttaccgacg aagcggagct ggcggggatg cctgaaagcg
     5761 cgctggctgc ggcaaaagcc caggccgaag cgaaagagct ggaaggctac ctgctgacgc
     5821 tggatatccc aagctacctg ccggtaatga cctactgcga caaccaggcc ttgcgtgaag
     5881 agatgtatcg cgcttacagc acccgcgcct ccgatcaagg cccgaacgcc ggtaaatggg
     5941 ataacagcaa ggtgatggaa gagatcctcg ctctgcgtca cgaactggcg caactgctgg
     6001 gctttgaaaa ctacgccttt aaatcccttg ccactaaaat ggcagaaaac ccgcagcagg
     6061 tgctggattt cttaaccgat ctggcaaaac gcgcgcgtcc gcaaggcgaa aaagagctgg
     6121 cacaactgcg cgccttcgcc aaagcggaat ttggcgtcga tgagttgcag ccgtgggata
     6181 tcgcgtacta cagcgaaaaa caaaaacagc acctctacag catcagtgac gaacagctgc
     6241 gtccgtactt cccggaaaac aaagcggtta acggcctgtt tgaagtggtg aaacgtattt
     6301 acggcatcac cgctaaagag cgtaaagatg ttgatgtctg gcatccggat gtacgtttct
     6361 tcgaactgta tgacgagaac aacgaactgc gcggcagctt ctacctcgat ctgtatgccc
     6421 gtgaaaacaa gcgcggcggg gcgtggatgg atgactgcgt aggccagatg cgtaaagctg
     6481 acggttctct gcaaaaaccg gtcgcgtatt tgacttgtaa cttcaaccgc ccggtaaatg
     6541 gtaaaccggc actgttcact cacgacgaag tgatcaccct gttccacgag ttcggtcacg
     6601 gcctgcacca tatgctgacc cgcatcgaaa ccgctggtgt ttccggtatc agcggtgtgc
     6661 cgtgggatgc ggtcgaactg ccgagtcagt ttatggaaaa ctggtgctgg gagccggagg
     6721 cgctggcgtt tatctctggt cactatgaaa ccggcgaacc gctgccgaaa gagttgctgg
     6781 ataaaatgct ggcggcgaag aactaccagg cggcgctgtt tattctgcgc cagctggagt
     6841 tcggcctgtt cgatttccgc ctccatgccg agttccgccc ggatcagggt gcgaaaatcc
     6901 tcgaaactct ggcagaaatc aagaaactgg ttgccgtagt accgtctcca tcctggggcc
     6961 gtttcccgca cgctttcagc catattttcg ccggtggtta tgccgcaggt tactacagct
     7021 acctgtgggc tgacgtactg gcggcagatg ccttctcgcg ctttgaggaa gagggcattt
     7081 tcaaccgtga aaccgggcag tcgttcctcg acaacattct gagccgtggc ggttcagaag
     7141 agccgatgga tctgttcaaa cgcttccgtg gtcgtgaacc gcagctggat gcgatgctgg
     7201 agcattacgg cattaagggc tgatcattca gtgaaaatct gcttaattga tgaaacaggc
     7261 gccggagacg gcgccttatc tgttctggcg gcccgctggg ggctggagca cgatgaagac
     7321 aacctgatgg cgctggtgtt aacgccggaa catctggaat tgcgcaagcg tgatgagcca
     7381 aaacttggcg gcatctttgt tgattttgtt ggcggagcga tggcgcaccg acgcaaattc
     7441 ggcggtggtc gcggtgaggc ggtggcgaaa gcggtgggca ttaaaggcga ttatttgccg
     7501 gatgtggtgg acgccaccgc agggctgggg cgcgatgcct ttgtactggc ttcagtcggc
     7561 tgccgcgtgc ggatgctgga gcgtaatcca gtggttgccg cgctactcga cgacggcctg
     7621 gcgcgtggtt atgcggatgc ggaaatcggc ggctggttgc aggagcggtt gcagttaatt
     7681 cacgcctcca gcctgacgac gctaaccgat attaccccac gcccgcaggt ggtctatctc
     7741 gatccgatgt tcccgcataa gcagaaaagc gcgctagtga aaaaagagat gcgtgtcttt
     7801 cagtcgctgg tgggaccgga tcttgatgcc gatggattac tggagcctgc acgcttgctg
     7861 gcgaccaaac gcgtggtggt aaagcgcccg gactacgcgc caccgctagc gaatgttacc
     7921 acgccaaacg cggttgtgac taaagggcat cgctttgata tctatgcagg tacgcccgtg
     7981 taaaagtaaa gcagggtagc gataacgcta ccctgctttc tgcaagaata attaatggct
     8041 ttccggcgtc gcaatcatgc gtttcagcca cggcaccatc aacagcatca ctactgcaac
     8101 gcccagcgtg accagaccaa tcttaccaaa cacgttggta tagacgggca acgtctcaag
     8161 cggatcggta atgttgtccg gcaccgcggt aaatgttgcc acatagccgc ccagcaagaa
     8221 cgcggcagcc tgcgtcagga accacatccc gagaataaag cccatcaaat gctgcggcac
     8281 cagggcagca atcatcgcca ggccaagggc gctaataaac agttcaccta agctctggaa
     8341 taagtacacc agcacgataa accatggcga tgtcagccct tgtgcatccg caaaccacat
     8401 tcccgcagct gccgccgtca aaaagcccag tgagcacata aacatgccga gagtaaattt
     8461 catcggcatc gagaggtctt tgcctttgtt acccagatgc gtgtaaatgc ctgccagtat
     8521 tgggctggcg agtaccaccc agaacgggtt aagcgcctgg aagctgaccg ggttgatgga
     8581 aaaaccgaga atttcatgat gcacgttgtt gatggcaaag aagttcagcg atgttggcat
     8641 ctgggcgtag agaatgtaaa acaccaccgc ttcgagcatc aggacaaagg cgacaaacat
     8701 tttattgcgc ccggttttat ccagcttgaa tgcctgacga aagaagatga tggtgacgac
     8761 gatggagaga acaatcagca ccagattggc gacttctacg ttgtgcatca gccatgcaca
     8821 tacgaagatc atcaccacgc tgccaagtaa tacgtacaac agtttgctga agctcatcgg
     8881 cttgaagtcg ggttcagaac caatgtcttt caccattcca cgacaggcga tgtaaaccag
     8941 taatgcgata attaaccccg ccccgcacag gttgtaggtg actgaataac cgaatctatc
     9001 agcgatcaca ggggccagcg ataacgctat caacgagccg atattgatcg acatatagaa
     9061 cagggtgaat gcgccatcaa gccgcggatc tttcggcgga tagcacttcg aaagcaagct
     9121 ggctgggtta gctttaaaca ggccgttacc gacagcgata gtccccaggg cgatgaaaat
     9181 caggtcaggc ttaagtagcg acatgccggt catgaagtag ccaatcgcca gcacaagtgc
     9241 tccgagaaca atggtgcgtt tggtccccag caggtggtcg ccgacatagc cgccaatgga
     9301 aatgaggcca tagaccagcg cagcaaaagc accaaaagtg acaaaagcct gctcttgcga
     9361 gaatccaagc tgtttaacga agaaaaccgc cagtacgccc tgcacgccgt agtagccgaa
     9421 tcgctcccat aactcgacaa aaaaggtcat gaaaaatggg cgaggttgct gcagcatccc
     9481 catgggtgtt gttgtattca tatttattaa ccttccaata ccatcctgaa aagtatgaac
     9541 gcgtaaggga taacgcgcga aaaagtttac atctgtcaga gattcttaat attgatgcgg
     9601 tgaatagatt aatacactat tacaacagaa aataaccagc taatcacact aatgatggaa
     9661 aatatgatgg tgttaaatgt tgtattggca ggagattttg attgcgtacg cagatcgtag
     9721 gccagataag gggtttacgc tgcatcaggt aacaaggcgc gccaaaaagc aaaacgcccg
     9781 catatgcggg cgttcaggaa cacgtcgtgg agggaagatt attcttcttc gtcgcgcagt
     9841 ggaacaatca gcatatcaac gtgaacggtg ttgatcagct gacgtgcgga agacatcagt
     9901 ttgctccaga agtcctggtg gtgaccacaa accaccagat ccatatcgta tttcttgatt
     9961 gcatcgacca gaacctggcc caggtcgccg ctgccgctca gggtttcagt gattgggtag
    10021 cctgcattag tggaaagctc ggtcagcgca tgatgtgtct cttcagagat gcgtttctgc
    10081 atatcaccca gattcacatc aataagcccg gtgtataggt cagagtagtt tacatctacg
    10141 tggatcagag aaactttcgc attgtagggg cgagccatag agactgcttt ctctaccaga
    10201 actttgcttt ccggggagag atcgaccgcg atgagaatgt gtttataagc catagtgtta
    10261 ctccttccat aaagttgtcg atgactggcc agctagcgtt tcttgtgccg ttacgtcacc
    10321 cgcgtcctgc gaactacgtg cgcggggcta acccagccgt aaagattcag tgttaagacc
    10381 atccttacct tatagcgacc cggatgatcc gtcaatccgc cttgcttacc cgttagataa
    10441 acaaattttc gacatgaata tattgatagt ggttaacctt ctggaaaaaa aacaacctga
    10501 tctcctacac tatctataga gccgctcgga tgttggtcac cacaaaagcg gtttctccag
    10561 gacctggtgt taactgattg gcagcgtatc gggagcggta gccccggggt ggaactccgc
    10621 gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc attattttgg gctttatgtg
    10681 tcgtttgcat tgttaacatg gcgcgctatt tctcatcact acgcgcgttg ttagtggtac
    10741 tgcgtaactg cgatccattg ctctatcaat atgttgatgg agggggcttt tttacctcac
    10801 atggccaacc caacaaacag gtgcgtctcg tttggtatat ctatgcccaa cgttatcgcg
    10861 atcatcacga tgatgagttt attcgccgct gtgagcgggt gcgtcggcag tttattctga
    10921 ctagcgcatt gtgtggtctg gtggtggtca gcctgattgc attgatgatt tggcattaag
    10981 cacttcagca ataataacgc ataaaaaaag cgggccagta aactgacccg ctcgagactg
    11041 attacaaacg caaaattgcc tgatgcgcta cgcttatcag gcctgcataa tctctgcaat
    11101 ctattgaatt tgcatgtttt tgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa
    11161 caaagcgcac tatgtcacaa tctgaagcgg gccagctgac ccgctcgttt tggtgcgtac
    11221 gattacagga actgcaagga gagccagtac agcccgccgg aaagcagtac cgcagccgga
    11281 agggtaaaca cccaggccat cagaatgctg gtcacggttt tacgctgtaa gccgccacca
    11341 tctaccacca tcgtccccgc gacagaagag gagagtacgt gagtagtgga aaccggcatc
    11401 ccggtataac tcgccaggcc gatagacact gccgccgtca tctgggcaga catcccctga
    11461 gcgtaggtca tgcctttctt accgattttc tcaccgatag tcgttgccac acgacgccag
    11521 ccaatcatcg taccgatacc taacgccagc gcgaccgcca tgatgatcca caccggtgca
    11581 tactcgatgg tgctaagcat gtcggacttc agtttcttca acaggcgctg atcgtcagca
    11641 ctcacgccag gtatcttcac tactttgtcg atagtgtcag aaacgcacag cataatgcgg
    11701 cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc tttccacatc ggtagttaac
    11761 atacctttca ggcggttgag cgcgttaatg gtattcgacg gatggcaatg gaactccgcg
    11821 ggttgcgttg cgccagcttc cggagccggt actaactgat cagcaccggt agcctgtttc
    11881 agcagcgcag gatgctgctc aaagtaagct tcgacgttgt tgatggcatc acgggtacgg
    11941 gtgatttcgt agccagtggc attcatattc accacgaagc ctgctggtgc gacaccaatc
    12001 aataccaaca taaccagacc aatgcctttc tgaccatcgt tcgcgccgtg cgaaaacgcc
    12061 acgccgatag cggaaaggat cagcgcaata cgcgtccaga acggcggctt tttcttgccg
    12121 tctttctttt cacgctccgc tggggtcagg tggatacggg cgcgtttctt ggtgccgctc
    12181 cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca ccaggccgac aataggggaa
    12241 acgatcagag aaccgaaaat acttaatact ttcgggatat tgagtgcatc caccactgac
    12301 gtcccggtca tcaacgcatt ggttaaacca atcccgatga tcgcgccaat cagtgtatga
    12361 gagctggatg caggtaaacc aaagtaccag gtacccaggt tccagataat cgccgccagc
    12421 aacatagaga acaccatggc aaggccatga gacgatccca tattaagcag cagatccgtc
    12481 ggcagcatat gcacaatggc ataggcaaca ctcagaccac ccagcaaaac acccaaaaag
    12541 ttgaacaccg ccgccataac cacggcgagc tgagaacgca tcgcgcgggt atagataacg
    12601 gttgccacgg cgttggctgt gtcatggaaa ccattgatgg cttcgtagaa cagcacaaaa
    12661 gccagtgcaa gcaataataa cagcccggta tgcaaatcca ggccagcaaa caaatgtagc
    12721 ataggacgtt acgccatttt gaggacatga acgcggcgca ttatcagtga ctttcgcggc
    12781 gcgggcaaag tgaaatatag actttttttg atttgcctcc tgtatggatt tcactcaaaa
    12841 aataattatc ttatataatt caggcaaata cttcctttta gtaatattga tgctggtgtg
    12901 accactgagg aatctttaca attcacgccc gttttttcta agaggagcgc aacgtggaaa
    12961 ggtttgatgc cattattata ggcgctggtg cggcgggtat gttctgttct gcgctggcag
    13021 gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa aaaaccaggg cgcaaaatcc
    13081 ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta tgtcgaacca ggcgcttatc
    13141 tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg ttttacccag tgggatttca
    13201 ttgatctggt caataaacac ggcatcgcct ggcacgagaa aacgttagga caactcttct
    13261 gcgatgactc cgcgcagcag attgtcgaca tgctggtgga tgagtgcgag aagggcaatg
    13321 taaccttcag attgcgtagc gaagtgctga gtgtggcgaa ggatgaaaca ggcttcacgc
    13381 ttgaactgaa cggcatgact gtcggttgcg aaaagctggt catcgcgacc ggtgggctgt
    13441 caatgccggg gctgggcgca tcgccgtttg gttataagat tgccgaacaa tttggcctca
    13501 acgtgctgcc gacccgtgcg ggtctggtgc cattcactct gcataaaccg ttgctcgaag
    13561 agttacaggt gctggcgggc gtggcggtgc cttccgtgat taccgctgaa aacggcaccg
    13621 ttttccgtga gaacttactc ttcacccacc gcggcttatc tggaccggcg gtgttgcaga
    13681 tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa tctgctaccg gatgtggacc
    13741 tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa tcagagcctg aaaaacacac
    13801 tggcggttca tctaccgaag cggttggttg aacgcttaca gcaactcggg caaatcccgg
    13861 atgtttcgct aaaacagctc aacgtgcgtg accaacaggc actgattagc acattgaccg
    13921 actggcgcgt acaacccaac ggcactgaag gctatcgcac tgccgaagtg acgctcggcg
    13981 gcgtggacac caacgaactc tcttcacgga cgatggaagc gcgcaaagtg cctgggctgt
    14041 acttcatcgg cgaagtgatg gacgttaccg gctggctggg gggctataac ttccagtggg
    14101 cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc agcgaagtcg tcctgacctg
    14161 ttacctaaag atgaaaatta ttagagccgc attaaaaatg agctaatttt gatagtggct
    14221 atcttgtgat tattttctaa tgagcccgtg aactgaaacc ctccaagctt aatataaggt
    14281 ggaaggaaag atgattgaaa actcacccag tggccagatc tttataaaat atgaacaggc
    14341 tgaaaaagac agtaactttg ttgttttttt atccttaacc actatgcatt aat
//
LOCUS       DAJTMK010000100        14324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTMK010000100 DAJTMK010000000
VERSION     DAJTMK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14324
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.101"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..161
                     /locus_tag="OO135_002605"
     CDS             <1..161
                     /locus_tag="OO135_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5398409.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="OO135_002606"
     CDS             complement(199..1260)
                     /locus_tag="OO135_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5398410.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMSVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="OO135_002607"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="OO135_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5398411.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="OO135_002608"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="OO135_002608"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5398412.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO135_002609"
     CDS             complement(3454..4200)
                     /gene="hda"
                     /locus_tag="OO135_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5398413.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO135_002610"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="OO135_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5398414.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO135_002611"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="OO135_002611"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5398415.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="OO135_002612"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="OO135_002612"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5398416.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTTSAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="OO135_002613"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="OO135_002613"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5398417.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="OO135_002614"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="OO135_002614"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5398418.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLSKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIREHDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="OO135_002615"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="OO135_002615"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5398419.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12123..14315)
                     /gene="pdeF"
                     /locus_tag="OO135_002616"
     CDS             complement(12123..14315)
                     /gene="pdeF"
                     /locus_tag="OO135_002616"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5398420.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPFLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQVLLVGNLIGVPLCYFIIRVVRNPFDLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT         3774 a         3673 c         3578 g         3299 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggtc agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc gatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatggc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actagcaggt tgccgtccag cgcctgaata atcagataca ccgcgaagca
      421 gctccagaat tccgtgcctg cgccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag aaccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccag gtggcgatcc ccaccacgct
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggcaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta acgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gattaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgaca aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaaaaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacga tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctag
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacaacg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctaatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acctcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgaccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctca atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagcctgca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaga aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgctccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aaccgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaaa ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgcg gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc ctcttctgcg tccagcacca cttcttcagg gcgcagcaca ctgatcagtt
     4321 tgaaaatcaa acttaagccg ataccgacga tggtcgccag cgccatacct tttaactccg
     4381 ccgcgccgat gtttaccttc gcgccactga cgccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt tcgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccgc
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtggagcca aagaagccgg agatcacggt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtagcccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac gaaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctga
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag cgaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcataccaa gacccgcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacgatttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga tcattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccac ttcagcggtg atcagcggca tcgcggaagg
     6961 ttgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgaa gattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgttctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccacg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta ttgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctcaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggtaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacgtcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcat ctgtaaatcg caagctccag cagttttttt ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gagcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctattgctg gagatggcgc gcaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggcttc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaggatgatt
     8881 acacctatct ggcggtggaa attatccgtg gagacaccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcgccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gctgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc cacatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacacgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgcgtggc gaaagattca cgcatcatcg actcgatgat ccacgccgca cataacggta
     9601 agaaagtcac tgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc agtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gcggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcacctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gcacgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttacgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgagtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgtggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga gcgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgc aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctacggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 aggtggcaaa tcgatgccac tatctgataa tccagactat ttgacacaat attgcggata
    12301 aaactgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaaaagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggctga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catgccatcc
    13021 caaaagaaac gaaattgctt gagatggcta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggggcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 acatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagagaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa atcaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aagacctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaaaaa ggcagaaaca gcggaagccc ccaccattta
    14281 tcacgtattt ttatataagt tgcattcagt ttcatagatg ctca
//
LOCUS       DAJTMK010000101        14291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTMK010000101 DAJTMK010000000
VERSION     DAJTMK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.102"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            470..1294
                     /gene="gadX"
                     /locus_tag="OO135_002617"
     CDS             470..1294
                     /gene="gadX"
                     /locus_tag="OO135_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5398421.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKFSSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFLIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1663..2391
                     /gene="gadW"
                     /locus_tag="OO135_002618"
     CDS             1663..2391
                     /gene="gadW"
                     /locus_tag="OO135_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5398422.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2754..5867)
                     /locus_tag="OO135_002619"
     CDS             complement(2754..5867)
                     /locus_tag="OO135_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5398423.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5892..7049)
                     /gene="mdtE"
                     /locus_tag="OO135_002620"
     CDS             complement(5892..7049)
                     /gene="mdtE"
                     /locus_tag="OO135_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR5398424.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7109..7387
                     /locus_tag="OO135_002621"
     CDS             7109..7387
                     /locus_tag="OO135_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398425.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7388..7915)
                     /gene="gadE"
                     /locus_tag="OO135_002622"
     CDS             complement(7388..7915)
                     /gene="gadE"
                     /locus_tag="OO135_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5398426.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHVPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8714..9286)
                     /gene="hdeD"
                     /locus_tag="OO135_002623"
     CDS             complement(8714..9286)
                     /gene="hdeD"
                     /locus_tag="OO135_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5398427.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9541..9873
                     /gene="hdeA"
                     /locus_tag="OO135_002624"
     CDS             9541..9873
                     /gene="hdeA"
                     /locus_tag="OO135_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5398428.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQVCTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9989..10315
                     /gene="hdeB"
                     /locus_tag="OO135_002625"
     CDS             9989..10315
                     /gene="hdeB"
                     /locus_tag="OO135_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5398429.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10379..11026
                     /locus_tag="OO135_002626"
     CDS             10379..11026
                     /locus_tag="OO135_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR5398430.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     MTAAIGMVIGSGMYELGMYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11068..11598)
                     /locus_tag="OO135_002627"
     CDS             complement(11068..11598)
                     /locus_tag="OO135_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5398431.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11754..12320)
                     /gene="slp"
                     /locus_tag="OO135_002628"
     CDS             complement(11754..12320)
                     /gene="slp"
                     /locus_tag="OO135_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5398432.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12675..13792)
                     /locus_tag="OO135_002629"
                     /pseudo
     CDS             complement(12675..13792)
                     /locus_tag="OO135_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3969 a         3025 c         3164 g         4133 t
ORIGIN      
        1 tgaactgttg ctggaagact acaaagcctc cctgaaatat ctcagcgatc acccgaaact
       61 gcagggtatt gcccagcaga acagctttaa acacacctga taacataacg ttgtaaaaac
      121 cgaatgccca gcctttaaaa aaacagctgg gcattcggtt gcttattaat cgcaataata
      181 tattggctgt ctattcatcg tgttgataag atatatgtta tatgaatgtt tattaaatgt
      241 agcgattgcc tttaatgtca cgaaagggga ttattttctt gctattaatt tcactgcgtt
      301 cttaaacacg tttttgtatt ataaatccag tcatcctgcc cgtagaacat cacacattat
      361 catcctgttc tcccgcttcg tttaaattta tttatcaatc aatttgactt aagagggcgg
      421 cgtgctacat taattaacag taatatgttt atgtaatatt aagtcaacta tgcaatcatt
      481 acatgggaat tgtctaattg cgtacgcaag acataaatat attctcacca tggttaatgg
      541 tgaatatcgc tattttaatg gtggtgacct ggtttttgcg gatgcaagcc aaattcgagt
      601 agataagtgt gttgaaaatt ttgtattagt atcaagggat acgctttcat tatttctgcc
      661 gatgctcaag gaggaggcat taaatcttca tgcacataaa aaattttctt cattactcgt
      721 tcatcactgt agcagagata ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa
      781 gaatcttcgc tatgcagaaa tgctacgtaa aagagcatta atctttgcgt tgttatctgt
      841 ttttcttgag gatgagcact ttataccgct gcttttgaac gttttgcaac cgaacatgcg
      901 aacaagagtt tgtacggtta tcaataataa tatcgcccat gagtggacac tagcccgaat
      961 cgccagcgag ctgttgatga gtccaagcct gttaaagaaa aaattgcgcg aagaagagac
     1021 atcatattca cagttgctta ctgagtgtag aatgcaacgt gctttgcaac ttattgttat
     1081 acatggcttt ttaattaagc gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt
     1141 catttacgtc tttcgaaatt attatgggat gacgcccaca gagtatcagg agcgatcggc
     1201 gcagggattg ccgaaccgtg actcggcggc aagtattgtt gcgcaaggaa atttttacgg
     1261 cactgaccgt tctgcggaag gaataagatt atagaatttt actcagacat aaaaaaaaac
     1321 ccggcatagg ggaccgggaa gaggatagtc tgccgtctcc agactaataa accgttataa
     1381 cactccctgt tggcacggga aactttgtgc tctcagtaag ttaaatataa cttttactgg
     1441 aaataagatc agccattttt ttataaacat aagctatacg ctgtgcgaaa atatattcgt
     1501 gctgcattta cttattatca attaactgtt atgcaaaact actttgtgga taaattttgg
     1561 tcctaccaaa tctggcagtt tttgcgctaa gaaacagtct ggcaacattt cattagtata
     1621 ctgaaattga aataatcgca gtatgaaata taagggataa tcatgactca tgtctgctcg
     1681 gtgatcctca ttcgtcgttc attcgatatt tatcatgaac agcataaaat atcgctgcat
     1741 aacgagagta tcgtgctgct ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg
     1801 gatacgcgac gactggatat cgatgagctg acagtttgcc attacttaca aaatattcgt
     1861 cagctaccac gcaatttagg gttacatagc aaagaccgtc tattaattaa ccagtcaccc
     1921 cccatgccgc tggtgacggc gatttttgat agcttcaatg aatccggggt aaattcaccg
     1981 atactgagca atatgctcta cctttcctgt ttatcgatgt tttctcataa gaaagaactg
     2041 atccccttac ttttcaatag catcagcact gtttcaggaa aagttgaacg ccttattagc
     2101 tttgatatcg ccaaacgttg gtatctgcgc gatatcgcgg aaagaatgta taccagcgag
     2161 agtctcatca aaaaaaagtt gcaggatgaa aatacctgtt tcagtaaaat attactcgct
     2221 tccaggatgt cgatggccag acgattactc gagttacgtc aaattcctct gcatactatt
     2281 gcagaaaaat gtggctatag cagtacgtcg tactttataa acacatttcg acaatattat
     2341 ggtgtaacgc cacatcagtt tgcgcaacat tcgccaggta ccttttcctg acatattttg
     2401 catttgaata ttggtcagga tctcacacct gcttcatgtg aaactcttcc ctgatgattt
     2461 ctgccgggct accggctagt tctctttcgc agtttgccag gcggtatagg tattttcagg
     2521 aggaaaagac tcggcatgtt tgggattatt aagctgacaa ttcatacgat tacgggaatg
     2581 tgggtcagta ttgtgctgtt caaattgatg acgaatggct ggagtggttt ctactttcag
     2641 tgctgtgtac taagcctggt attcctgacg gttagctggt tgttgagtgg tgaatggtta
     2701 gcaggaaaga gtaaggctga accttcacgt tcaaccttac tctcatttac acgttacgct
     2761 tttttaaagc gggcaaagag atgttccact acaacgaaaa agaccggaac gaagtaaatt
     2821 gccagcactg ttgcggcaaa catcccgccc atcacgccgg tacctaccgc gttttgcgcg
     2881 ccagaaccgg caccatgact gataaccagc ggcagcacgc cgagaataaa ggccagagag
     2941 gtcatcagga ttgggcgtaa acgcatccgc gccgcttcga tgattgcccc tatcggcgtt
     3001 ttcccttctt tctgcatcat ctcaacggca aattcgacaa tcaggatggc gttcttggcg
     3061 gaaagcccga tggtggtcag caaaccaacc tggaagtaga cgtcattact taagccgcgc
     3121 agatcggtgg ccagtaatgc gccaacgacg cctaacggaa caaccaacat caccgagaac
     3181 ggaattgacc agctctcata gagtgcggcg agggcgagga acaccacgac cagtgaaatg
     3241 gcatacagtg caggagcctg gtttgaggat aacgcttcct gatacgacag tccggtccat
     3301 gagtagccga cgcctgccgg aagtttagcg accaggtctg ccataaattt catagcgtca
     3361 ccggtacttt tcccggccgc cgcttcacct aaaatctcca ttgacgggat gccgttgtag
     3421 cgttccaatc gtggtgaacc ataggtccat tcggtagacg agtaggcaga aagcggtgcc
     3481 atcgtgccag aggcgttgcg tacataccat tggttgatgt tatccggcaa catacggaac
     3541 ggcgtgcctg cctggacata cacttttttc acccgcccct ggttgaggaa gtcgttcacg
     3601 tagctgctgc cgaaggcggt ggaaattgtc tggttgatat cagacagcgc cacacccatt
     3661 gcctcagctt tcgcggcgtt gacgttcact ttgaacatcg gcgtatcttc caggccgttc
     3721 gggcgtaccc cggtgacctg gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt
     3781 gcctgggtta gtttttcgtg ccccaggtta ccgttgtcca gcagttccat atcaaaacct
     3841 gacgcggtac ccagttcagc caccgcgggt aagttgaacg ggaagacgac ggctttattg
     3901 atactgctta acgcaatcat tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg
     3961 acacgttcag accacggctt gagactgata aacgccaggc cgttgttttg cccctggccg
     4021 ctgaagccaa agccgccaac ggtaaacacc gactgaacat tatctttctc ttttgtaaga
     4081 taataatccg tcacctgttg cagcactttc gtggtgttaa ccatggtggc accggaaggt
     4141 aactgcgcgg tggtcataaa taccccctga tcctcttctg gtaagaaaga ggtcggtgta
     4201 cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt agaccaccat gtagcgaccg
     4261 gtacaacgca acagcgagcg ggtgctatcg gtatagtgct gagttgattt ttcaaacagc
     4321 gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc cttccggcgc ggctttcaga
     4381 atggtggcgc acagggcagg ggtcaggctc attgccacaa atactgaaag cagcatggag
     4441 gagatcagcg tgatggagaa ctggcggtag atctccccgg ttgcgccgct cataaaggcc
     4501 atcggcataa acactgcgga aagaacaaca gcaataccga ccagcgcacg ttggatctgc
     4561 cccatcgatt tatgcgtcgc ttccttcggt ggtagcttat cttccgcaat gacacgctcg
     4621 acgttctcca ccaccacgat ggcgtcatcc accagtaacc ctatcgccag caccatcccg
     4681 aacatagtta acgtgttgat ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga
     4741 ataaccaccg gtacggcaat cgtcgggatg attgtggcac ggaaattctg caaaaacaga
     4801 tacatgacca ggaagacgag gatgatagcc tcaaccagtg ttttaaaaac ttcctgaata
     4861 gaaatttcga taaacggcgt ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa
     4921 taggctgata agcggttcag ctcctctttg actgcccgcg aggtatccag ggcgtttgct
     4981 ccggtagcca gtttgatggc gatcccggca gcaggtttgc cgttatagcg cgccacggtg
     5041 gaataatctt ccgctccaag ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca
     5101 tcttgctgaa ctttcaacag gattttgcca aattcttccg gcgtttgcag acgcgtctgc
     5161 acaatgatcg aggcgtttag ttgctggtct gccgcctgtg gcatgccacc cagttgacca
     5221 ccggaaatct ggttgttttg caccttaatc tgggaaataa catcggaagg taccagattg
     5281 tatttattga gtttttgcgg gtccagccag atacgcatgg cgtactcgga accaaagagc
     5341 tgtacgctac caacgcccgc ggtacggctt agcgggtctt tgatattaga cgctacatag
     5401 tccgcgatat cgtactggtt gaggctgccg ttatcagaaa taaacgccgc taccatcaga
     5461 atattactgc tcgacttatc aacgctaatc ccctgctgct gcaccgcttc aggtaatgaa
     5521 ggcatagcga gttgcagttt attttgcacc tgaacctgtg cgatatcagg agatgtccca
     5581 gtctcgaagg tcagagtgat agaggcatta cccgccgcat cactggttga agacatgtac
     5641 atcaggccat caagcccatt catattttgc tcaatcacct gagtgaccga gtcttctacc
     5701 gtttgcgcat cggcacctgg ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc
     5761 ggatactgcg caaccggtaa gttcatgatc gccagaccac ctgcaagcat cataataatg
     5821 gcaagtaccc aggcaaaaac cgggcgatca ataaaatagt tagccatgaa agtcccctta
     5881 agcctgcaac gttattgttt cgattcggtg ctggcgtttt cctggctgga ggaaattgct
     5941 cgtgctttga tacccggacg aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc
     6001 tgcaagccag aggtgacaac ccactggtcg ccgatggctt tgctggcttc aatttcgcgt
     6061 agctgcacaa catcgtcttt atccagaatg agcgccgttg ctttaccctg ggcgttgtgg
     6121 gtgacgcctt cctgcggcac cagtaataca ttctggcggc taccttcatc cactaatgcc
     6181 gtgacgtaca tgccaggcag caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc
     6241 acggagcccg tggtttcatc caccgtcggg tcggagaatt tcagtgtgcc ggtctggctg
     6301 tagcgtttac cattttccag attgagctgt actggcgtac tgccctgaac ctgtttgatt
     6361 tgcccactgg cgacctcttc tttcatgcgc aagaaatcct gcaccgactg cgtgagatcg
     6421 acataaatcg ggtccagacg ttgtacggta accagcgaat ctgcctgatt agcggtaacg
     6481 agtgcgccga cggtcaccga cgatttcccg ctgacgcccg taatcggcga ggtgacattc
     6541 gcgtattgca gattgatcgt cgcctgttca acagccgctt tggcgacggt gacattggct
     6601 tctgcttcat tcagctgggt gcgcgcggtg tcgtaatcct gacggctaac gtagttggtc
     6661 ttcagcaacg atgcctggcg gttaaaggtg atgcgggcat tgctggcggt agagagcgct
     6721 ttcgccagcg agcctttggc ggagtttagc tcggcctgta aaggtgcagg atcaatctga
     6781 tacagcgaat cgccctggtt cactttatcg ccttcgataa agttgcgttt aatgataata
     6841 ccgcccacct ggggacgtat ctcggcaact tcataaggaa cggttctacc gggcaattcg
     6901 ctcaacacat tgaccgaacc gggggagagt gtgacgacac cgacctcagg cgtcatggcg
     6961 gcggcgtttt ccgccgattt gtcatcgcag gcggtgagca tcgcgccgca gaataacaac
     7021 ggtattaaca gctttcttct tctgttcatt ttagtccctg aaaattcttg agaaataacg
     7081 gttctttaaa ttgcgcgcga ggagagatat gagggattta cagaccagcg gcatagtggg
     7141 gttgtcagcc agtaaggtgg gatacaggca cagtgatcga catggtgagg tcaacgacaa
     7201 aaaaaatgtt gccgttctgc caacagttcc tgccagcatt cgggcaacga agggttctac
     7261 tggtggatac acataccagg ggaataaagt cgttttagcc tctcctttag taatcaccgg
     7321 gggtaatgaa atcagcatct gcatccctcg tcatgccagc catcaatttc agttgcttat
     7381 gtcctgacta aaaataagat gtgataccca gggtgacgat gtcgctcata cgtcttagtt
     7441 tcaacttgta cataatgttg aatttctgaa tctttaatgt ttgttgcgaa atattgagcg
     7501 tcaacgtgat attgctttca tttttatttt tcagaatcag ttgcataatt ttgaactgat
     7561 tatgtgaaaa acataattgt gaattcttat ggggcacgtg tttaccataa gtaatctcaa
     7621 gaagattttt gatactttct ttgcggctaa caaaataaat aggcgcttta gcttttagtt
     7681 tactgatgtg ataaggtgcc aaaacaataa ttctctcggc atctaatttc tccagaaatt
     7741 taatcgcttc ttcatcaagg atatgattgt gataggtatc aatgataacc ttgaagggtt
     7801 tattgcctac ttttttgatc tctgacaggg aattgatttt tatcatttcg tgattatctt
     7861 tcaactgcca aaagccctgt aaaagaaaag aatctttcgt catgagaaaa atcataactt
     7921 gctccttagc cgttatcgtt tcgaacatgt catccttttc attatttaca tccttgtccg
     7981 aatcgttgtt caatatagta aacgcctata agaactttac ccttcgtgaa ctaatacgtc
     8041 tattaacact aatcaatact tcagatttat ccatcacgca ctccatatat tgatcctaag
     8101 taagattcct ggttgttatc agcttgtaaa tagtaaacaa aaagttaacg tttgcggatt
     8161 actgcccaag aataagttat caataaccag tttgtgatct ctgaagaata ttactaaagt
     8221 taaaatgtaa tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga
     8281 ttgccagctt tcttttaacg cattgaaatt acctgagtaa ataacgtatt catgtattca
     8341 ggtttttata tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca
     8401 aaaaaatgaa cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt
     8461 tgttcacttt cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat
     8521 gataatgttt gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag
     8581 gttgaaataa tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacac
     8641 tgcgtagcgg agagagtatt aatcggatca tagtcacatc aagtgactat gatccgggtg
     8701 acaaccgggg taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg
     8761 cgctaaatat cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg
     8821 ttgcaccaag gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc
     8881 agctgccttt cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac
     8941 agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga
     9001 agaaatagcc gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga
     9061 aattatgact gcggttgctg aataacccga caataagcgc aatacccgag cagattaata
     9121 atgcacccac tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca
     9181 acccgacgat aaacagcagc acggcaataa actggattgc tctgcgatgt tttttaagca
     9241 tctccagatc aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata
     9301 aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgcagt
     9361 cgatttaata aaaatttcct aattgcagta tctgatgcat ctgtaactca ttgtattgaa
     9421 ataaaaatat ctgattttga tattttccat caacatgaca tatacagaaa accaggttat
     9481 aacctcagtg tcgaaattga ttcgtgacgg ctctttcact ttatagttga ggatattacg
     9541 atgaaaaaag tattaggcgt tattcttggt ggtctgcttc ttctgccagt tgtgagcaat
     9601 gcagcggatg cgcaaaaagc agctgataac aaaaaaccgg tcaactcctg gacctgtgaa
     9661 gatttcctgg ctgtggacga atccttccag ccaactgcag ttggttttgc tgaagcgctg
     9721 aacaacaaag ataaaccaga agatgcggtt ttagatgttc agggtattgc aaccgtaacc
     9781 ccagctatcg ttcaggtttg tactcaggat aaacaagcca actttaaaga taaagttaaa
     9841 ggcgaatggg acaaaattaa gaaagatatg taattccggg aatgcgttac atcgtacttc
     9901 cttgcatatt gaacaggccg gaatatcttc tttaaaagca gctattcctc ctgttcatat
     9961 ataatctata tattgaatgg gttacaaaat gaatatttca tctctccgta aagcgtttat
    10021 ttttatgggc gctgtagcgg ctttgtcact ggtgaacgca caatctgcgc tggcagccaa
    10081 tgaatccgct aaagatatga cctgccagga atttattgat ctgaatccaa aagcaatgac
    10141 cccggttgca tggtggatgc tgcatgaaga aacagtatat aaaggtggcg ataccgttac
    10201 tttaaatgaa accgatctca ctcaaattcc taaagtgatc gaatactgta agaaaaaccc
    10261 gcagaaaaat ttgtatacct tcaaaaatca agcatctaat gacttgccga attaatgagg
    10321 tacaagtaaa aaggagtagc aagttgagcc atcttgctgc tcctttttgc atttttatat
    10381 gacagcagaa tttattatac gtcttatact cgcggcaatt gcctgtggcg ctattggcat
    10441 ggaaaggcaa atgcgaggca aaggagcagg gttacgcaca catgtattaa ttggcatggg
    10501 aagcgccctg tttatgattg tttcgaaata tggttttgct gacgtgctgt ctttagatca
    10561 cgtcggactc gaccccagcc gtattgctgc tcaggtggtg acgggcgtcg ggtttatcgg
    10621 tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt ttgacgacgg cagcggatat
    10681 ctggatgacc gccgctatag gtatggttat tggcagtggt atgtacgaat tgggtatgta
    10741 tggttcagtg atgaccttgc tggtgctgga agtcttccat caattaacct tccgcctgat
    10801 gaacaaaaat taccatttac agctgacatt agtgaacggt aatacggtat caatgctcga
    10861 ctggtttaag cagcaaaaaa taaaaacgga tttagtttcg ttgcaggaaa atgaagatca
    10921 tgaagtggtc gccattgata tccagcttca tgccacaacg tcgatagaag atctgttgcg
    10981 attattaaaa ggtatggcag gggtgaaagg cgtttcaatt agctaatctg tgtggcagcg
    11041 tagtcagact caccgtaagc ctgaaattca caccagataa tcaatatgct gatggcgtac
    11101 cagctcattg atccgcttaa gattgaggat catcatgata ttgtgtttat ggcagtagaa
    11161 cgtttttagc gttattttca gtttctccag aatatcctcc gttgtcattt gattcgcaat
    11221 gcagctcaga acctgatgtt gcttgcgcga aagtgacaca gccgcttcag gcacagagca
    11281 gtaactgcca ttgatgagtg cgacaaaatc gatgatagtg atagtccgcg gaacaaaggt
    11341 caccggcact ttccccaggc agcgtttaat attaaagggg gagaaaataa tcacatggac
    11401 aggttttaat cgttcaattt gagtgagaaa attagaatga aatacattat tcattaatgt
    11461 atcaataatg acaaaggcat tctgatgcaa cattccgtcg atctcttctt cgttctgtat
    11521 atgaacgaca ttacctttgc tcaaaatgtt tttcatcgcg gtgaagaaca tcgtatccct
    11581 ggtaattata agaaacataa ccgactcctg gtgcggacat taattttata atcagtttgc
    11641 caaaggtaat taacaggaca gggcttataa taataatagt tagcttgctg tgtattaatt
    11701 ttatgtaatg cacgatgcag atgctgagaa agcatctgca tctttcggtg gtgttatttg
    11761 accagctcag gtgttacctg actcaccgca ttggtgtagt aaggcgcacc ccagcccggt
    11821 tccggccaga atgcaccata gccgtaatcc cataggttat aggtggtatt aaccacttct
    11881 ctcaaatgcc acacctggat gccctgcata ttcacttcca ggaagttata cgggacttta
    11941 ttgataaagc caggttgttc accctgaatg gtaccgagga tggtaacaaa gtgattacga
    12001 tagttcactg ggtcaaggaa gccgctttgt ctggcgagca gtcggccctg atagttggct
    12061 tcaatatcag gtttcgcata gctatccaac ggtaatacag cgatttctaa caacgtatcc
    12121 gttttgccat tgataacgtt gataaccttc ccaccaaagc gcgcttgttg accaacatat
    12181 aaccccggct ggttatgaac agcaacaaaa cttttttgaa tatcaggttg gttattgcct
    12241 ttgatatttt gcggaattga actacatgcg gcaagcaaaa atgaaaggct gaggatgagt
    12301 gcaccttttg tcatgttcat gttactatcc ttatcaacaa atattaaaac cataatagat
    12361 atttctttat agttttcatc tgattctgag gcgatatgcc tgatagtaac gtaaacctgt
    12421 ctgatgttta tatccctttt gtttagttca attccattga ttttggggcg tggcataagt
    12481 atccatcgcc ccataatata atcctttgtt ttaaaattac ataaaatcta tttttattca
    12541 ataattataa agtgctaata atcaatatta tggtattata ttctgataat gatttgataa
    12601 gcgaattctt ttgccagatg gtatttctat ttccacccca taatttatat ctttttcatt
    12661 aggaagtggc ctctttaaat aaggcgtgtg tgacattttt tttgggttaa gcgtctcgaa
    12721 ttgaaatact tcgaactgat attcagacat ttctgcccat gtttgaattt tattgtatcg
    12781 agagagtgtc gtagaggctt taacttgctt ataacattgt ttttggaatt cttgctgata
    12841 ttttaatatg tcattgtatg aaagattggt tatcctggcc tctaaaaatt taattattgt
    12901 cgaagcaatt tccgcttttg ctcgtaaaaa gttgtcatta atggttaata atgttgaaaa
    12961 taaaccttcg atttgtttgt caagatattg accatcttta tataatttgg ggggtagggt
    13021 gttctttatg taaaaaaata gtgtcaccta aatttgcgtc agtgatattt atattaagta
    13081 gatcttcgaa ataaaacctt gacaaggtta gaacgaaccc ggaaaggttc tgtcctgtca
    13141 tattgaaatc aataaagttg gttccggcaa agactttcat cctgttttta ttttcactgt
    13201 gctcaatata taacttcgtc attttttgat acaggctttg aatatctttt atattagttg
    13261 gtaagtcagc tcgttgttca gcaataaaat tagtatctga aaacatggtt tggacactga
    13321 atgttatggg tattccctct tctacgctaa ttttagttat gattgccttg tcgggtttag
    13381 gattagcgac aacaatgatt cgacattcag cacttttgtt tttttcatat ctaatgttat
    13441 taatctgatt aagtattgat tgtgtctgtt gcgtaagtgg tgtcgaactt atcgtttcaa
    13501 tgttattaat aagacttaca agttgaacca caacttttga gtagtctgca tttgtatatg
    13561 tggtgttcgt gtggcatgca ttaatttgtt ggagttgttg ttgcgctaaa tcaaattgtt
    13621 cagggttgta tccttcttca gagaatgtgg aattaatggg ttgttgttct tcccaattat
    13681 cacaaggcca cttcggtgtg tctggcgttt taacattatc ggtatttgtt gtgcagttat
    13741 aaatatcgcc gtgatatgta tttattatac ctggggtaaa gtcaattgac ataacattta
    13801 ttattcatca tctgttttat gctaaggttt ttaaattgga taattagaac aggaatggat
    13861 tttcttctac acacaataaa tataatgcgt tatatcttcc tgatataatt acctttaaat
    13921 tccctttcat ttccacccgg taatatttgt gtataaaaaa agcagtgata gtatcgtaaa
    13981 ggaaaacgtt aacaactatc aaaaagcgct cattttaaaa agttattatg ttctccgata
    14041 gtgtgtgaag taatattttt gtgatgtttt tgatgtgtaa taatgaacca ggttaaagtg
    14101 tatttaaaaa tgtgttgtat tagcggctga aatctgcctt ttaaaaagga ggagggtact
    14161 gagacaaaga gtcattgctt ctccgataaa atgtcagaga agcatgatta cgtggttatt
    14221 gtctgttgat gacttgttga ccgagccgtc atcattctca atgcattagt taaatcattt
    14281 gtgattttta a
//
LOCUS       DAJTMK010000102        14083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTMK010000102 DAJTMK010000000
VERSION     DAJTMK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14083
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.103"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(177..389)
                     /gene="rpmE"
                     /locus_tag="OO135_002630"
     CDS             complement(177..389)
                     /gene="rpmE"
                     /locus_tag="OO135_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5398433.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            592..2790
                     /gene="priA"
                     /locus_tag="OO135_002631"
     CDS             592..2790
                     /gene="priA"
                     /locus_tag="OO135_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5398434.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSLKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2946..3971
                     /gene="cytR"
                     /locus_tag="OO135_002632"
     CDS             2946..3971
                     /gene="cytR"
                     /locus_tag="OO135_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5398435.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4063..5022
                     /gene="ftsN"
                     /locus_tag="OO135_002633"
     CDS             4063..5022
                     /gene="ftsN"
                     /locus_tag="OO135_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5398436.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAL
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5115..5645
                     /gene="hslV"
                     /locus_tag="OO135_002634"
     CDS             5115..5645
                     /gene="hslV"
                     /locus_tag="OO135_002634"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5398437.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5655..6986
                     /gene="hslU"
                     /locus_tag="OO135_002635"
     CDS             5655..6986
                     /gene="hslU"
                     /locus_tag="OO135_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5398438.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7053..7979
                     /gene="menA"
                     /locus_tag="OO135_002636"
     CDS             7053..7979
                     /gene="menA"
                     /locus_tag="OO135_002636"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5398439.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8072..8557
                     /gene="rraA"
                     /locus_tag="OO135_002637"
     CDS             8072..8557
                     /gene="rraA"
                     /locus_tag="OO135_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5398440.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="OO135_002638"
     CDS             complement(8642..8887)
                     /gene="zapB"
                     /locus_tag="OO135_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5398441.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9313..10158
                     /gene="glpF"
                     /locus_tag="OO135_002639"
     CDS             9313..10158
                     /gene="glpF"
                     /locus_tag="OO135_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR5398442.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10181..11689
                     /gene="glpK"
                     /locus_tag="OO135_002640"
     CDS             10181..11689
                     /gene="glpK"
                     /locus_tag="OO135_002640"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5398443.1"
                     /translation="MAEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11824..12834
                     /gene="glpX"
                     /locus_tag="OO135_002641"
     CDS             11824..12834
                     /gene="glpX"
                     /locus_tag="OO135_002641"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5398444.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGKQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12931..13677
                     /gene="fpr"
                     /locus_tag="OO135_002642"
     CDS             12931..13677
                     /gene="fpr"
                     /locus_tag="OO135_002642"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5398445.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     HDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13682..>14083)
                     /gene="uspD"
                     /locus_tag="OO135_002643"
     CDS             complement(13682..>14083)
                     /gene="uspD"
                     /locus_tag="OO135_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5398446.1"
                     /translation="PSGNEEDALLVNKALELARHNDAHLTLIHIDDGLSELYPGIYFP
                     ATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKEQCDLLVCGH
                     HHSFINRLMPAYRGMINKMSADLLIVPFIDK"
BASE COUNT         3383 a         3620 c         3812 g         3268 t
ORIGIN      
        1 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
       61 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      121 ggcaaaattc aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      181 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      241 tcacgctgtt tgccagtaaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      301 tgaccaacag tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      361 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      421 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      481 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      541 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      601 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      661 atgacggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      721 ggggttgtgg tgtcagttag cgatgtcagt gagctgccgc ttaacgagct gaaagcggta
      781 gtggaagtgc tggacgttga gccggtattt actcactccg tctggcggtt actgctctgg
      841 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      901 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
      961 ggccaggcgg tggatctgaa cagcctgaaa cgctccccca agcaacaaca ggcgctggcg
     1021 gcgttacggc aaggcaaaat ctggcgcgac caggtcgcca cgctcgaatt taatgatgcc
     1081 gcgttgcagg cgctacgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1141 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag
     1201 gccaccgccg ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg
     1261 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1321 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1381 cgttttcgtg aacgttttaa tgcccccgtg gaagttctgc attccggcct gaacgacagc
     1441 gagcggcttt cggcgtggtt gaaagcgaaa aatggcgagg cggcgattgt gatcggcacc
     1501 cgctccgcgc tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga tgaagagcac
     1561 gacagctcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat
     1621 cgtgcgcaca gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg
     1681 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacccgtcg ggcagggaat
     1741 gcgcgtccgg caattcaaca tgtgctggat ttaaaaggtc agaaggtgca agcaggtctg
     1801 gctccggcgt taatcacccg tatgcgccag catttacagg ccaacaatca ggttatcctc
     1861 tttcttaatc gtcggggttt tgcgcctgcg ctgctgtgcc acgactgcgg ctggatagcc
     1921 gaatgcccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     1981 caccactgtg acagtcagcg tccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2041 ctggtccccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gatgttcccc
     2101 ggcgtgccca tttcacgtat cgaccgcgat accaccagcc tcaaaggggc gctggaacag
     2161 caactggcgg aagtgcatcg cggcggcgcg aggattttga tcggcacaca aatgctggcg
     2221 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
     2281 ttttctgccg attttcgctc ggcagaacgt ttcgcccagc tttacaccca ggtcgccggt
     2341 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat
     2401 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2461 gagcgacgca tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2521 cataacaatc agcacgcgcc gctgttcctg caacaactgc gtaatctgat cctttccagc
     2581 ccgctggcag acgacaaact gtggattctc ggcccggttc cggctctggc acctaagcgt
     2641 ggtggccgct ggcgctggca gatcttgttg cagcaccctt cccgcgtgcg cttgcaacac
     2701 atcattagtg gtacgctggc gcttattaat acaataccgg attcccgcaa ggtgaaatgg
     2761 gtgttggatg tcgatcccat cgaaggctaa tgcgcaaacg ttgcgaggcg gatcgaaaaa
     2821 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2881 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtacc aggcgaggag
     2941 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3001 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3061 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg
     3121 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3181 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3241 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3301 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3361 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
     3421 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
     3481 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccactgt
     3541 gtcactaccg cctgcaaggc tatgttcagg cgctgcgtcg ctgcggaatt atggttgatc
     3601 cacaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagca atgcaacagc
     3661 tgcttgatct tccacaaccg cctactgcag tcttctgcca tagcgatgtg atggcgctcg
     3721 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3781 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3841 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3901 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     3961 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4021 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4081 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4141 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4201 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4261 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatt
     4321 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4381 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4441 gctgatatgc gccagcagcc aacgcagctg gttgaggttc cgtggaatga acagacgccg
     4501 gaacagcgtc agcaaacgct acaacgccag cgtcaggcgc tgcaattagc ggagcagcaa
     4561 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4621 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cagcttctac tcagcagccg
     4681 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4741 gcgccggttg ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4801 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4861 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4921 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     4981 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5041 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5101 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5161 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5221 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5281 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gttaaagccg
     5341 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5401 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5461 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgcg
     5521 cgctgttaga aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg
     5581 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5641 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5701 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5761 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5821 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5881 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctatgtc ggtaaggaag
     5941 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6001 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6061 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6121 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6181 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6241 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6301 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6361 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6421 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6481 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6541 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6601 ctgacctaat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcactga
     6661 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6721 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
     6781 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6841 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6901 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     6961 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7021 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7081 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7141 tcgggacggc gctggcatgg tggcaaggtc attttgatcc gctggtcgcc ctgctggcac
     7201 tgattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7261 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
     7321 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7381 ggctggcact ggttgcagtg gcgtgccata cgctggctga ttttgtcggt ttcctgattc
     7441 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7501 atatcggtct gggggatatt tccgtactgg ttttctttgg ctggttaagc gtcatgggga
     7561 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7621 gcctgctggc aacggcagta ctgaacatta ataacctgcg tgatatcaat agcgaccgcg
     7681 aaaacggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7741 cctgcctgct gatgggctct ctggtatgtc tggcgctgtt taatctcttt tcgctgcata
     7801 gcctgtgggg ctggctgttc ctgctggcgg caccgttgct ggtgaagcaa gcccgttatg
     7861 tgatgcggga aatggacccg gtggcgatgc gaccgatgct ggaacgcact gtcaaggggg
     7921 cgttactgac taacctgctg tttattttag ggatattcct aagccagtgg gcagcataac
     7981 tgacaaatat caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg
     8041 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8101 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8161 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8221 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8281 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8341 ggttatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8401 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8461 cgtcaatttt ggcggtgtaa ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8521 gattattctt tcagaagatc cgctggatat tgaatgataa gaaaggcacc gcaaggtgcc
     8581 ttttttctgc gttacctgtt ggcctacaca gtaaagaaat tacgcgaaag atgaagcgta
     8641 atcagacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct
     8701 gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa
     8761 cttcctgcga cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag
     8821 tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca
     8881 ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaaatcctcg tcccgattac
     8941 cggtgacgcc ttaataaata cgagcgcact ttagttagct ccgattgtat gaagccgcgc
     9001 catcgctgtc cagcggcacg ccttgcagat tacggtttgc cacacttttc atccttctcc
     9061 tagtgacata atccacatca atcgaaaatg ttaataaatt tgttgcgcga atgatctaac
     9121 aaacatgcat catgtacaat cagatggaat aaatggcgcg ataacgctca ttttatgacg
     9181 aagcacacac attttaagtt cgatatttct cgtttttgct cgttaacgat aagtttacag
     9241 catgcctaca agcatcgtgg aggtccgtga ctttcacgca tacaacaaac attaactctt
     9301 caggatccga ttatgagtca aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc
     9361 ggtaccgggt tgttgatttt tttcggtgtg ggttgcgttg cagcactaaa agtcgctggt
     9421 gcgtcttttg gtcagtggga aatcagtgtc atttggggac tgggggtggc aatggctatc
     9481 tacctgaccg caggggtttc cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg
     9541 ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta tcgtttcaca agttgccggc
     9601 gctttctgtg ctgcggcttt agtttacggg ctttactaca atttattttt cgacttcgag
     9661 cagactcatc acattgttcg cggcagcgtt gaaagtgttg atctggcagg cactttctct
     9721 acttacccta atcctcatat caattttgtg caggctttcg cagttgagat ggtgattacc
     9781 gctattctga tggggctgat cctggcgtta acggacgatg gcaacggtgt accacgcggc
     9841 cctttggctc ccttgctgat tggtctactg attgcggtca ttggcgcatc tatgggccca
     9901 ttgacgggtt ttgccatgaa cccagcgcgt gacttcggtc cgaaagtctt tgcctggctg
     9961 gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca ttccttactt cctggtgccg
    10021 ctttttggcc ctatcgttgg cgcgattgta ggtgcatttg cctaccgcaa actgattggt
    10081 cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa
    10141 caaaaagctt cgctgtaata tgactacggg acaattaaac atggctgaaa aaaaatatat
    10201 cgttgcgctc gaccagggca ccaccagctc ccgcgcggtc gtaatggatc acgatgccaa
    10261 tatcattagc gtgtcgcagc gcgaatttga gcaaatctac ccaaaaccag gttgggtaga
    10321 acacgaccca atggaaatct gggccaccca aagctccacg ctggtagaag tgctggcgaa
    10381 agccgatatc agttccgatc aaattgcagc tatcggtatt acgaaccagc gtgaaaccac
    10441 tattgtctgg gaaaaagaaa ccggcaagcc tatctataac gccattgtct ggcagtgccg
    10501 tcgtaccgca gaaatctgcg agcatttaaa acgtgacggt ttagaagatt atatccgcag
    10561 caataccggt ctggtgattg acccgtactt ttctggcacc aaagtgaagt ggatcctcga
    10621 ccatgtggaa ggctctcgcg agcgtgcacg tcgtggtgaa ttgctgtttg gtacggttga
    10681 tacgtggctt atctggaaaa tgactcaggg ccgtgtccat gtgaccgatt acaccaacgc
    10741 ctctcgtacc atgttgttca acatccatac cctggactgg gacgacaaaa tgctggaagt
    10801 gctggatatt ccgcgcgaga tgctgccaga agtgcgtcgt tcttccgaag tatacggtca
    10861 gactaacatt ggcggcaaag gcggcacgcg tattccaatc tccgggatcg ccggtgacca
    10921 gcaggccgcg ctgtttggtc agttgtgcgt gaaagaaggg atggcgaaga acacctatgg
    10981 cactggctgc tttatgctga tgaacactgg cgagaaagcg gtgaaatcag aaaacggcct
    11041 gctgaccacc atcgcctgcg gcccgactgg cgaagtgaac tatgcgttgg aaggtgcggt
    11101 gtttatggca ggcgcatcca ttcagtggct gcgcgatgag atgaagttga ttaacgacgc
    11161 ctacgattcc gaatatttcg ccaccaaagt gcaaaacacc aatggtgtgt atgtggttcc
    11221 ggcatttacc gggctgggtg cgccgtactg ggacccgtat gcgcgcgggg cgattttcgg
    11281 tctgactcgt ggggtgaacg ctaaccacat tatacgcgcg acgctggagt ctattgctta
    11341 tcagacgcgt gacgtgctgg aagcgatgca ggccgactct ggtatccgtc tgcacgccct
    11401 gcgcgtggat ggtggcgcag tagcaaacaa tttcctgatg cagttccagt ccgatattct
    11461 cggcacccgc gttgagcgcc cggaagtgcg cgaagtcacc gcattgggtg cggcctatct
    11521 cgcaggcctg gcggttggct tctggcagaa cctcgacgag ctgcaagaga aagcggtgat
    11581 tgagcgcgag ttccgtccag gcatcgaaac cactgagcgt aattaccgtt acgcaggctg
    11641 gaaaaaagcg gtcaaacgcg cgatggcgtg ggaagaacac gacgagtaat gtaaatgccg
    11701 gatgaagcgt ttatgccgca tccggtagtc ctgaaacgtg cgggggcaac cccgcacaca
    11761 tcaataatcc cacccttccc ctgtgctaca cttcgcgcca ttccttactg cttagagttt
    11821 gctatgagac gagaacttgc catcgaattt tcccgcgtca ccgaatctgc ggcgctggct
    11881 ggctacaaat ggttaggacg cggcgataaa aacaccgcgg acggcgcggc ggtaaacgcc
    11941 atgcgtatta tgctcaacca ggtcaacatt gacggcacca tcgtcattgg tgaaggtgaa
    12001 atcgacgaag caccgatgct ctacattggt gaaaaagtcg gtactggtcg cggcgacgcg
    12061 gtagatattg ctgttgatcc gattgaaggc acgcgcatga cggcgatggg ccaggctaac
    12121 gcgctggcgg tgctggcagt aggcgataaa ggctgcttcc tcaatgcgcc ggatatgtat
    12181 atggagaagc tgattgtcgg gccgggagcc aaaggcacca ttgatctgaa cctgccgctg
    12241 gcggataacc tgcgcaatgt agcggcggcg ctcggcaaac cgttgagcga actgacggta
    12301 acgattctag ctaaaccacg ccacgatgcc gttatcgctg aaatgcagca actcggcgta
    12361 cgcgtatttg ctattccgga tggcgacgtt gcggcctcaa ttctcacctg tatgccagac
    12421 agcgaagttg acgtgctgta cggtattggt ggcgcgccgg aaggcgtagt ttctgcggcg
    12481 gtgatccgcg cattagatgg cgacatgaac ggtcgtctgc tggcgcgtca tgacgtcaaa
    12541 ggcgacaacg aagagaatcg tcgcattggc aagcaggagc tggcacgctg caaagcgatg
    12601 ggcatcgaag ccggtaaagt attgcgcctg ggcgatatgg cgcgcagcga taacgtcatc
    12661 ttctctgcca ccggtattac caaaggcgat ctgctggaag gcattagccg caaaggcaat
    12721 atcgcgacta ccgaaacgct gctgatccgc ggcaagtcac gcaccattcg ccgcattcag
    12781 tccatccact atctggatcg caaagacccg gaaatgcagg tgcacatcct ctgattgatt
    12841 tgatcgattg agccttccag tccttcggga ctggaatttt tttgttcgga gaacgaagat
    12901 aaggcaagtc aatcaaaaca ggagaaaaac atggctgatt gggtaacagg caaagtcact
    12961 aaagtgcaga actggaccga cgccctgttt agtctcaccg ttcacgcccc cgtgcttccg
    13021 tttaccgccg ggcaattcac caagcttggc cttgaaatcg acggcgaacg cgtccagcgc
    13081 gcctactcct atgttaactc gcccgataat cccgatctgg agttttacct ggtcaccgtc
    13141 cccgatggca aattaagccc acgattggcg gcactgaaac caggcgatga agtgcaggtg
    13201 gttagcgaag cagctggctt ctttgttctg gatgaagtac cagattgcga aacgctatgg
    13261 atgctggcaa ccggtacagc gattggccct tatttatcga ttctgcaact aggcaaagat
    13321 ttagatcgct tcaaaaatct ggtcctggtt cacgccgcac gttatgccgc cgacttaagc
    13381 tatttgccac tgatgcaaga actggaaaaa cgctacgaag gcaaactgcg cattcagacg
    13441 gtggtcagtc gggaaacggc agcggggtcg ctcaccggac ggataccggc attaattgaa
    13501 agtggggaac tggaaagcgc gattggcctg ccgatgaata aagaaaccag ccatgtgatg
    13561 ctgtgcggca atccacagat ggtgcacgat acacaacagt tgctgaaaga gacccggcag
    13621 atgacgaaac atttacgtcg ccgaccgggc catatgacag cggagcatta ctggtaagcg
    13681 gttacttatc gataaacggc acgatgagca aatccgcact catcttattg atcatcccgc
    13741 gatatgccgg catcaaacgg ttgataaatg agtgatgatg accacagaca aggaggtcgc
    13801 actgctcttt ttgcataatt tccagcagtg tttccggcat ttctccgcgt tcaatacgca
    13861 gttttgtctt cggccattga atatttttcg tcagtttata cagcttgtta tccgacttat
    13921 tcttcaacaa ttgaagaata tcttctgttg cagggaagta gatacccgga tacaactcgc
    13981 ttaagccatc atcaatatga attaacgtca ggtgagcgtc attatgtctg gcgagctcca
    14041 gagctttatt caccagtaag gcatcttctt cattcccgga agg
//
LOCUS       DAJTMK010000103        14049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTMK010000103 DAJTMK010000000
VERSION     DAJTMK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.104"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..69
                     /locus_tag="OO135_002644"
     CDS             <1..69
                     /locus_tag="OO135_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398447.1"
                     /translation="FSKSVELHDKVIGHYLNIKHYQ"
     gene            227..271
                     /locus_tag="OO135_002645"
     CDS             227..271
                     /locus_tag="OO135_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398448.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            524..844
                     /gene="iraM"
                     /locus_tag="OO135_002646"
     CDS             524..844
                     /gene="iraM"
                     /locus_tag="OO135_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5398449.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     SICY"
     gene            complement(912..2162)
                     /gene="icd"
                     /locus_tag="OO135_002647"
     CDS             complement(912..2162)
                     /gene="icd"
                     /locus_tag="OO135_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5398450.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVTIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            2334..2987
                     /gene="rluE"
                     /locus_tag="OO135_002648"
     CDS             2334..2987
                     /gene="rluE"
                     /locus_tag="OO135_002648"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5398451.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2997..3458
                     /gene="nudJ"
                     /locus_tag="OO135_002649"
     CDS             2997..3458
                     /gene="nudJ"
                     /locus_tag="OO135_002649"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5398452.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3512..4618
                     /gene="mnmA"
                     /locus_tag="OO135_002650"
     CDS             3512..4618
                     /gene="mnmA"
                     /locus_tag="OO135_002650"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5398453.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4654..5295
                     /gene="hflD"
                     /locus_tag="OO135_002651"
     CDS             4654..5295
                     /gene="hflD"
                     /locus_tag="OO135_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5398454.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            5299..6669
                     /gene="purB"
                     /locus_tag="OO135_002652"
     CDS             5299..6669
                     /gene="purB"
                     /locus_tag="OO135_002652"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5398455.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVPNFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6837..7508
                     /gene="phoP"
                     /locus_tag="OO135_002653"
     CDS             6837..7508
                     /gene="phoP"
                     /locus_tag="OO135_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5398456.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7508..8968
                     /gene="phoQ"
                     /locus_tag="OO135_002654"
     CDS             7508..8968
                     /gene="phoQ"
                     /locus_tag="OO135_002654"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5398457.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            9044..10165
                     /gene="roxA"
                     /locus_tag="OO135_002655"
                     /gene_synonym="ycfD"
     CDS             9044..10165
                     /gene="roxA"
                     /locus_tag="OO135_002655"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5398458.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGERLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(10214..11440)
                     /gene="pepT"
                     /locus_tag="OO135_002656"
     CDS             complement(10214..11440)
                     /gene="pepT"
                     /locus_tag="OO135_002656"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5398459.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11690..12826
                     /gene="potA"
                     /locus_tag="OO135_002657"
     CDS             11690..12826
                     /gene="potA"
                     /locus_tag="OO135_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5398460.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12810..13673
                     /gene="potB"
                     /locus_tag="OO135_002658"
     CDS             12810..13673
                     /gene="potB"
                     /locus_tag="OO135_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5398461.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(13905..13976)
                     /locus_tag="OO135_002659"
                     /pseudo
     CDS             complement(13905..13976)
                     /locus_tag="OO135_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         3518 a         3501 c         3457 g         3573 t
ORIGIN      
        1 ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac
       61 tatcaataag ttggagtcat taccgtgttt tggtttagtc atcatccagt tatatgtatt
      121 ttagccagga acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat
      181 gtcatgttta tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt
      241 ggcacctagt agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat
      301 aacattatag ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc
      361 attttgttta acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt
      421 tttaattaat ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca
      481 tccaaatgaa ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat
      541 tgacacggta attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa
      601 aatgatcatc tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt
      661 taagcttggt attatccttc aggaaaaaga atatcctatt actatttata acattgcacc
      721 attcaacaag aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag
      781 caaaataaca aataaatgtt tacataatag ttgcattata aaaatatgct cgatatgcta
      841 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac
      901 tcatggatag attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc
      961 ttagcgcctt ccatcagacg ttcaaagtca taagttacgg ttttcgcgtt gatagcacct
     1021 tccatacctt taacaattaa atcagccgct tcagtccacc ccatatggcg cagcatcatc
     1081 tcagcggaga ggatgataga gcctggattt actttgtcct gaccggcgta tttcggcgca
     1141 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggggcgata
     1201 ccgataccgc caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata
     1261 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
     1321 atcacgtctt taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg
     1381 ccgccgtcga tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta
     1441 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga
     1501 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa
     1561 cacggcttaa taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct
     1621 tcacgcagga atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc
     1681 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1741 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1801 tcctggcgca gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga
     1861 cctttaatgg taacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1921 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1981 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     2041 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     2101 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     2161 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     2221 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     2281 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     2341 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac ctggttaagc
     2401 gattcagctc gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc
     2461 tgttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     2521 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg
     2581 aagggttact ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggca
     2641 aacgcaccgg gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2701 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc
     2761 tggttgacga accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2821 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2881 tgaccgccca tgttggtttc cccacgctgc gactgattcg ttatgcgatg ggtgattaca
     2941 ctttggataa ccttgccaat ggcgaatggc gagaagcaac agaataagga tgcacaatgt
     3001 ttaaaccgca cgttaccgtt gcttgcgtag tgcacgcaga gggtaaattt ttagtcgttg
     3061 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     3121 aaaccttagt ggaagctgcc gcccgtgagc tatgggaaga aaccggcatc agcgcgcaac
     3181 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     3241 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     3301 actgctgtcg ttgggtcagc gccgaagaaa ttttaaaggc gtcaaatctt cgttcgccgc
     3361 tggtggcgga aagtatccgt tgttatcaaa gcgggcaacg ctatccgctg gagatgattg
     3421 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     3481 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     3541 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     3601 tcaggttgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3661 agcggcagcg gatctggctg atgcccaggc tgtctgcgac aagctcggca ttgaactgca
     3721 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3781 taaagccggt cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc
     3841 tttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3901 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3961 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     4021 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     4081 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct
     4141 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     4201 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     4261 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     4321 tctggttgtt gcgcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     4381 gcagttgcac tgggtcgatc gcgaaccatt caccggtact atgcgttgca cggtaaaaac
     4441 ccgctatcgc cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga
     4501 agtgattttc gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     4561 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     4621 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4681 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4741 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4801 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4861 ctcggcgtgc tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca
     4921 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4981 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     5041 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     5101 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgccggcgaa agttcgcgca
     5161 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg
     5221 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     5281 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     5341 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     5401 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     5461 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt ccccaatttc
     5521 agcgaagaag atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa
     5581 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     5641 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5701 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5761 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5821 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag
     5881 taccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5941 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     6001 acctcgctgg gtattcagtg gaacccgtac actacccaga ttgaaccgca cgactacatt
     6061 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     6121 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     6181 tcttccacca tgccgcataa agttaacccg atcgacttcg agaactccga agggaatctg
     6241 ggcctttcca acgcggtatt acagcatctg gcaagcaaac tgccggtttc ccgctggcag
     6301 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     6361 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     6421 ctggatgagc tggatcacaa ctgggaagtg ctggcggagc caatccagac agttatgcgt
     6481 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc tgactcgtgg taagcgcgtt
     6541 gacgccgaag gcatgaagca gtttatcgac ggtctggcgt tgccagaaga agagaaagcc
     6601 cgcctgaaag cgatgacgcc ggcaaactac attggtcgcg ccatcaccat ggttgatgag
     6661 ctgaaataaa ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta
     6721 tccgcttttt attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc
     6781 cataaccaca taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc
     6841 gcgtactggt tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg
     6901 atgctggtca tcaggtcgat gatgcagaag atgccaaaga agccgattat tatctcaatg
     6961 aacatttacc ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac
     7021 tgattcgccg ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg
     7081 aaagctggca ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac
     7141 catttcatat tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc
     7201 tggcttcaca ggtcatttcg ctaccaccgt ttcaggttga tctctctcgc cgtgaattat
     7261 ctattaatga cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga
     7321 tacgcaataa tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg
     7381 cggagctgcg ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc
     7441 aggcacaata tccgcaagaa gtgattacta ccgttcgcgg ccagggctat ttgttcgaat
     7501 tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct
     7561 gttggcaacg gctgcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat
     7621 cggttatagc gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct
     7681 gttctatacc cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat
     7741 cgacaagcaa agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc
     7801 gcaacgtgac gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg
     7861 ttttcatgaa attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc
     7921 gatacagcaa cagttgcagg aagtgcggga agatgatgac gacgcggaga tgacccactc
     7981 ggtggcagta aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt
     8041 ggataccatt ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt
     8101 gctctcagcc aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt
     8161 acgccccatc gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt
     8221 gctcaatcca gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt
     8281 aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag
     8341 tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat
     8401 gagcgtcagt gatgctgaac cggtaatgct ggagcaaatc agccgcattt cacagcaaat
     8461 tggctactac ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca
     8521 tccggtcgcc ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa
     8581 aggggtcaat atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga
     8641 ttttgtcgag gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt
     8701 cgaaatttct gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc
     8761 cggtattcca ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt
     8821 acgccctggg caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg
     8881 taaaatcgtc gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg
     8941 ccagcattct gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca
     9001 tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac
     9061 tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg
     9121 cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga
     9181 aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg
     9241 cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt
     9301 gaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg
     9361 gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct
     9421 cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga
     9481 aaggctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga
     9541 agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc
     9601 gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggctttc gcgcgccaaa
     9661 tacgcgggaa ctgattagtg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa
     9721 ctactacagc gatcctgatg ttccacctcg cgctcatcct gcggatgttc tgcctcaaga
     9781 gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca
     9841 atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc
     9901 gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct
     9961 gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga
    10021 ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa
    10081 ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag
    10141 cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg
    10201 catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca
    10261 ccgctttttc cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc
    10321 cagtgaacaa gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc
    10381 cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg catcgcctgc tgggcgatat
    10441 cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca
    10501 ccagttcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca
    10561 ttttacattt acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat
    10621 cggcccgttc aacggtgcct ttcatgcccg ccaggtgata gaaaccttca tagccttctg
    10681 tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg
    10741 catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat
    10801 tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag
    10861 tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt
    10921 cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata
    10981 ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt
    11041 taccatcggt ggtaatcaac gtctgaccca gtagctgatg cagcaccggg aacataaccg
    11101 gtgataaaac ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa
    11161 tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc
    11221 caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct
    11281 cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata
    11341 acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca
    11401 gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg
    11461 tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg
    11521 caagcgggaa tatttatcag cattacctta atgaatataa agctaagcca gtaattcaca
    11581 acaaagattg gcgattcaag cggtttgccg tagaattaca gccctcatta agagtcacca
    11641 aggtggttaa ccacaaaccc cgcatcggta agccatccgt tgcgtttaca tgggacagag
    11701 taaaaaattg aataaacaac cgagttcgct ttcaccgctg gtgcaattgg cgggaattcg
    11761 caaatgcttt gatggtaaag aggtcattcc ccagctggat ctgactatca acaatggcga
    11821 gttcctcacg ctgcttggcc cttctggctg cggtaaaaca accgttcttc gcctgattgc
    11881 aggtctggaa actgttgatt ccggacgcat catgctggat aacgaggaca tcacccacgt
    11941 tccggcggaa aaccgctatg tgaacactgt tttccaaagc tacgcacttt tcccccacat
    12001 gaccgtgttc gaaaatgtgg cctttgggtt gcgcatgcaa aaaacccccg ctgctgaaat
    12061 tacgccccgc gtgatggaag ccctgcggat ggtgcaactg gaaaccttcg ctcaacgtaa
    12121 accgcatcag ctctctggtg gtcaacagca acgcgtcgcc attgctcgcg cggtggttaa
    12181 caagcctcgt ctgttgttgc tggatgagtc gctctcagcg ctggattaca aactgcgtaa
    12241 gcaaatgcag aacgagctga aagcgttaca gcgtaagctt ggcattacat tcgtcttcgt
    12301 gactcacgac caggaagaag cactcaccat gtcagacagg attgtggtga tgcgcgaggg
    12361 tcgcattgag caagacggca cgccgcgtga aatctacgaa gagccgaaaa acctgtttgt
    12421 tgccggcttc attggcgaaa ttaatatgtt taacgccacg gtgatcgagc gactcgacga
    12481 gcagcgcgta cgcgccaacg ttgaaggccg cgaatgtaat atctacgtta acttcgccgt
    12541 tgaaccgggg caaaaactgc atgttctgct gcgcccggaa gacttacgtg ttgaagagat
    12601 taacgacgac aaccacgctg aagggctgat tggttacgtt cgtgagcgta actacaaagg
    12661 catgacgctg gagtcggttg ttgaactgga aaatggcaag atggtgatgg tcagcgaatt
    12721 cttcaatgaa gacgatcctg actttgacca ctctctcgac caaaaaatgg ccattaattg
    12781 ggtagaaagc tgggaggtcg tactggctga tgaagaacac aagtaagttc cagaatgtag
    12841 tgattgtcac tattgtcggt tggcttgtgt tgtttgtctt tctgcccaac ctgatgatca
    12901 ttggcaccag ctttttgacc cgcgacgacg ccagtttcgt caaaatggtc tttacgctgg
    12961 ataactacac gcgtctgctc gatccgctct attttgaagt gctattgcac tcgctgaata
    13021 tggcgctgat cgccaccctc gcctgcctgg tgctgggcta tccatttgcc tggtttctgg
    13081 cgaagctgcc acacaaggtg cgtccgctgc tgctgtttct gctgattgtt ccgttctgga
    13141 ccaactcatt gattcgtatc tacgggctga aaattttcct cagcaccaaa ggctatctca
    13201 acgagttttt gctctggctg ggcgttatcg acacaccaat ccgtatcatg ttcacgccca
    13261 gtgcggtgat tatcggtctg gtttacattc tgctgccgtt tatggtgatg ccgctgtact
    13321 ccagtatcga aaaactggat aagccgttgc ttgaggcggc gcgcgatctc ggtgccagca
    13381 agttacagac ttttatccgc atcattattc cactgacgat gccggggatt attgccggat
    13441 gtctgctggt gatgctgcca gcgatgggcc tgttctatgt gtccgacctg atgggcggtg
    13501 cgaaaaacct gctgatcggt aacgtcatca aggtacagtt cctcaatatt cgtgactggc
    13561 cgtttggtgc agccaccagc attacgctga ctatcgtaat gggcctgatg ttgctggttt
    13621 actggcgcgc ttctcgtttg ctgaataaga aggtgagcga attagatgat taatcattta
    13681 tattcaatgc attaagtttg tttttgagtg gttttgccgt ttaaggtaac ggcgttatag
    13741 atcctatcaa aatcaatcaa ttccattctg gttttggaga aagatttttc gagagcttcg
    13801 gtctttaact aaaactgatt gcgtataacc ccaatacccc tccccgacta tctgaatggt
    13861 cggggaggca cttcacgcac aaaatatcat ccctgcgccg ttgattactc tgcaggtgaa
    13921 gtcggccact caatatcagg tgcagttgat gtatcaacac ggttcagcaa cacctgaagt
    13981 ggcacactga atttggccac ctgaacagag gtgatatgct cacctcagaa caacacaggt
    14041 gctccaatg
//
LOCUS       DAJTMK010000104        14006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTMK010000104 DAJTMK010000000
VERSION     DAJTMK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14006
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.105"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..422
                     /locus_tag="OO135_002660"
     CDS             <1..422
                     /locus_tag="OO135_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5398462.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFHLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            438..920
                     /locus_tag="OO135_002661"
     CDS             438..920
                     /locus_tag="OO135_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5398463.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGITHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENLRVAVVITTP
                     "
     gene            complement(997..1344)
                     /gene="rplS"
                     /locus_tag="OO135_002662"
     CDS             complement(997..1344)
                     /gene="rplS"
                     /locus_tag="OO135_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5398464.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVYSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1386..2153)
                     /gene="trmD"
                     /locus_tag="OO135_002663"
     CDS             complement(1386..2153)
                     /gene="trmD"
                     /locus_tag="OO135_002663"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5398465.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2184..2732)
                     /gene="rimM"
                     /locus_tag="OO135_002664"
     CDS             complement(2184..2732)
                     /gene="rimM"
                     /locus_tag="OO135_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5398466.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2751..2999)
                     /gene="rpsP"
                     /locus_tag="OO135_002665"
     CDS             complement(2751..2999)
                     /gene="rpsP"
                     /locus_tag="OO135_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5398467.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3136..4497)
                     /gene="ffh"
                     /locus_tag="OO135_002666"
     CDS             complement(3136..4497)
                     /gene="ffh"
                     /locus_tag="OO135_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5398468.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4664..5455
                     /locus_tag="OO135_002667"
     CDS             4664..5455
                     /locus_tag="OO135_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5398469.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5476..6762
                     /locus_tag="OO135_002668"
     CDS             5476..6762
                     /locus_tag="OO135_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5398470.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6817..7410)
                     /gene="grpE"
                     /locus_tag="OO135_002669"
     CDS             complement(6817..7410)
                     /gene="grpE"
                     /locus_tag="OO135_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5398471.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7533..8411
                     /gene="nadK"
                     /locus_tag="OO135_002670"
     CDS             7533..8411
                     /gene="nadK"
                     /locus_tag="OO135_002670"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5398472.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8497..10158
                     /gene="recN"
                     /locus_tag="OO135_002671"
     CDS             8497..10158
                     /gene="recN"
                     /locus_tag="OO135_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5398473.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10307..10648
                     /gene="bamE"
                     /locus_tag="OO135_002672"
     CDS             10307..10648
                     /gene="bamE"
                     /locus_tag="OO135_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5398474.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10710..11000)
                     /locus_tag="OO135_002673"
     CDS             complement(10710..11000)
                     /locus_tag="OO135_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5398475.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10990..11427)
                     /gene="ratA"
                     /locus_tag="OO135_002674"
     CDS             complement(10990..11427)
                     /gene="ratA"
                     /locus_tag="OO135_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5398476.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11598..12080
                     /gene="smpB"
                     /locus_tag="OO135_002675"
     CDS             11598..12080
                     /gene="smpB"
                     /locus_tag="OO135_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5398477.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIGEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12295..12657
                     /gene="ssrA"
                     /locus_tag="OO135_002676"
     tmRNA           12295..12657
                     /gene="ssrA"
                     /locus_tag="OO135_002676"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12818..14006)
                     /locus_tag="OO135_002677"
                     /pseudo
     CDS             complement(12818..14006)
                     /locus_tag="OO135_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3459 a         3780 c         3319 g         3448 t
ORIGIN      
        1 cgttgatgaa caattccgat gcccgaaaaa cgtcggcgtt actatttctt gatggcgata
       61 atttcaaata tatcaatgat acctggggtc atgcgacggg cgatagggtc ttgattgaaa
      121 tcgcaaaacg gttagctgaa tttggcgggc tgcgacataa agcataccgc ctgggcggcg
      181 atgaattcgc tatggtgctc tatgatgtac agtcggaatc tgaagtgcaa cagatatgct
      241 cagcactgac acaaatcttt aatctcccgt ttcatcttca taatggccat cagaccacca
      301 tgacattaag cattggttac gcgatgacca ttgagcacgc ctctgcggaa aaattacaag
      361 agcttgccga tcacaatatg tatcaggcca aacaccagcg tgccgaaaag ctggtgagat
      421 aacaaggata tatcgatatg ataaagcacc tggtagcacc cctgattttc acctcactaa
      481 tactgactgg ctgccagtcc cctcagggaa agtttactcc tgagcaagtc gccgctatgc
      541 aatcttatgg atttactgaa tccgccggcg actggtcgct gggcttatca gatgccattc
      601 tgttcgcaaa aaatgactac aaattgctcc cggaaagcca gcaacagatc caaaccatgg
      661 cagccaaatt ggcctcgaca gggataacac atgcccgtat ggatggacac accgataact
      721 atggtgaaga cagttacaac gaaggcttat cattgaaacg ggcgaatgtc gtggccgatg
      781 catgggctat tggtggacaa attccacgca gcaatctcac cacacagggt ttaggaaaaa
      841 aatatcccat agccagtaac aagaccgccc agggccgcgc cgagaacctc cgcgtcgcag
      901 tggtgattac aaccccttaa aacacgagca aacagacaaa aaaagggcca gccaattggc
      961 cagcccttct taacaggatg tcgcttaagc gaaatcttag ttaagacgct ctttgatacg
     1021 agcagcctta ccagtacgct cacgcaggta gtacagttta gctttacgaa cagcaccacg
     1081 acgtttgaca gaaatgctgt aaactaccgg agagtgagtc tggaagacac gctcaacgcc
     1141 ttcgccgttg gaaattttac gaacagtgaa tgcagagtgc agaccgcggt tacgaatagc
     1201 gataaccacg ccctcgaatg cctgcagacg ttttttggaa ccttcaacaa cccatacttt
     1261 cacttccacg gtatcacccg gacggaagga aggtacgtcc tgcttcatct gctcttgttc
     1321 aagttgctta ataatgttgc tcataattta atctcttatc ctgggtaaac tgatatctcg
     1381 ggggcttacg ccatcccatc atgtttatgt tgctgttgtg cgtgttccgt tttgaactcc
     1441 gccagcaacc ttgcttgctc ttcagtcaga gccaggtttt ccagaagttc aggtcttcta
     1501 agccaggtac ggcccagcga ctgtttcaaa cgccagcgac gtatctcggc atggttgccc
     1561 gacagtaaca ctggcggaac ttccatccct tctaacacct caggccgcgt atagtgcggg
     1621 caatccagca atccttcagc aaaggaatct tccgttgccg aggcttcatg tcccagtacc
     1681 cccggaataa accgggaaac ggagtcaatc agcgtcattg ctggtaactc accaccactg
     1741 agaacgtaat cgccgattga ccattcttcg tcaatttcgg tttggatcac gcgctcatct
     1801 ataccttcgt agcgaccgca caccagaatc aatttttgat tcgttgccaa ttcgctgacg
     1861 cccgcttgat caagcttgcg tccctgtggt gacagataaa tcacctttgc gccttcaccc
     1921 gccgcggctt ttgctgcatg aatggcgtcc cgcaagggtt gcaccatcat taacatcccc
     1981 ggtccgccgc cgtaaggacg atcgtccacg gtacggtgcc ggtcatgcgt gaagtcgcga
     2041 ggactccagc tctggatgct cagcaggcca tttttaactg cccggccagt taccccgtaa
     2101 tcggtaattg cgcggaacat ttcaggaaac aggctaatta tgccaatcca catagcgccg
     2161 tcttttaccg tttatccggt ggtttaaaaa ccaggatccc aatctacttc gattgaacga
     2221 gtagtgagat cgactttctt gataacctgc ccatcgagga acggtacgag acgttccttg
     2281 ataccaaacg catctttcag gtttgcctta atgacgagaa cgtcattaga tccggtttcc
     2341 atcatatcga cgactttacc gagatcgtag ccttcagtgg ttactacctg gcagcccatc
     2401 aggtctttcc agtagtagtc accctcttca agctgaggca actgcgatga atccacgaca
     2461 atttcacaat tcgtcagcag gttcgccgca tcacgatcgt caacgccttt cagcttgatg
     2521 atcatgtcct gattgtggtg cttccagctt tccagctgga cttgctgcca ctgacccgcc
     2581 ttctggataa accagggctg atagtcaaaa atgctttcgg cgtcttcggt ggaagaaaac
     2641 actctgagcc acccacgaat accgtaagac gaacccattt ttcccaaaac gatgggatca
     2701 acaggtgctt gcgcggtgag ttgtttgctc atcatgacca ccgtgacaga ttaagctgct
     2761 ttgtttactt ctttgatcag cgcagcaacg cgatcagaaa tagttgcgcc ctggccaacc
     2821 cagtgagcga tgcgatccag atccaggcga gtgccttctt ctttttcgct agcgattggg
     2881 ttgaagaaac caacgcgctc gatgaagcga ccgttgcgtg cattacggct gtctgcgaca
     2941 acaacctggt agaacggacg ctttttagcg ccgtgacgtg ctaaacgaat agttaccata
     3001 acatcctctt gtgtgaataa aacaaccgga ccccatcgag gaacggagtc cggtgtcata
     3061 ttaaaagccc gaaaatttta ctgatttctg gagaaaatgc aatcagcagt taataactct
     3121 gtcgcataag gtcatttagc gaccaggaaa tcccggcggc atcatcccct tcatgcttct
     3181 catcatcttc gccattccgc ccttcttcat tttcttcatc atgcgctgca tgtcgtcgaa
     3241 ctgtttcaga agacggttaa cgtcctgcac ctgcataccg caaccggcag caatacggcg
     3301 tttacgcgaa cctttgatga tttctggctt agcgcgctct ttcatcgtca tcgagttgat
     3361 gatggcttcc atacgcacca gcactttatc gtccatctgt gacttgacgt tatccgggat
     3421 ctgccccatg cccggcagct tgcccatcag gctggccatg ccgcccatat ttttcatctg
     3481 gcgcagctgc tcaagaaagt cgttgagatc gaagccgtca ccttttttca gcttgctggc
     3541 taatttctct gcctgcgcgc ggtcaacttt gctttcgata tcttcgatca gcgacagtac
     3601 gtcgcccatg ccgagaatac gcgacgcaat gcggtccgga tggaacggct ccagcgcctc
     3661 agttttctcg ccaacaccga ggaacttgat cggtttgcca gtgatgtgac gaatagagag
     3721 cgccgcaccg ccgcgggcat cgccgtccac tttggtcaac actacgccgg taagcggtaa
     3781 cgcttcattg aatgcttttg ccgtattggc cgcatcctga ccggtcatgg cgtcaaccac
     3841 aaacagggtt tcaaccgggt taatcgacgc atggacttgt ttgatctcgt ccatcatcgc
     3901 ttcgtcaacg tgcagacgac cagcggtatc caccagcagc acgtcgtaga atttcagttt
     3961 ggcttctttc agcgccgcgt taacgatatc taccggcttc tgaccaacat cagaagggaa
     4021 gaaatcaacg cccacctgct ctgccagcgt ctcaagctgt ttgattgccg ccgggcgata
     4081 aacgtcggca gaaaccacca gcactttctt cttgtgcttc tcgcgcagga acttaccgag
     4141 tttaccaacg ctggttgttt taccggcacc ttgcaggccc gccatcagca cgaccgcagg
     4201 cggttgcgcc gccaggttca gggtctggtt ctcttcgccc atcgccgcaa ccagttcgtt
     4261 acggacgatt ttgacgaact cctgccccgg cgtcaggctc ttattaactt catgaccaac
     4321 cgctttctct tttacgcgat tgataaactc acgcactacc ggcagagcga catccgcctc
     4381 cagcagcgcc atgcgcactt cgcgcagcgt atcttttacg ttgtcttcag ttaggcgtcc
     4441 acggccactg atattgcgca gcgtgcgcga caaacgatcg gttaaattat caaacattgt
     4501 ctctcgcctg gggtggaaac ggttggccgc aatcgcgaca catcatcagt attttgccgc
     4561 agtataacat gaaggcgtct ttgttgttat gcaacggttg gagcagcgtt cacctgacgc
     4621 tatactgctt ctctttctta ttgctcaaac tgtcgacatc actatgcccg tttttgctct
     4681 gctcgcgctt gtcgcctact ccgtcagtct tgcgctgatt gttcccggtc tgctgcaaaa
     4741 aaacggcggc tggcggcgca tggctattat ttctgcggtc attgcgctgg tctgccacgc
     4801 aatcgctctg gaagcccgca tcctgcccga cggtgatagc ggacaaaacc tcagcctgct
     4861 gaacgttggt tcattggtca gtttgatgat ctgtacggta atgaccattg tggcttctcg
     4921 caatcgtggc tggttgctgc tacccattgt ctatgccttt gcgcttatca acctggcgct
     4981 ggcgaccttc atgcccaatg aatacatcac ccatctggaa gcaacgcctg ggatgctggt
     5041 gcacattggc ttatcgctct tttcctatgc cacgctaatt atcgccgccc tgtacgcgct
     5101 gcaactggcg tggattgatt accaactgaa gaacaagaag ctggcattta accaggaaat
     5161 gccgccattg atgagtatcg agcgtaaaat gttccacatc acgcagattg gcgtggtgct
     5221 gctaacgctc acgctttgca ctggcctgtt ctacatgcac aacctattta gcatggaaaa
     5281 tatcgacaag gctgtgctct ctatcgtggc gtggtttgtc tatattgtgc tgctgtgggg
     5341 acattaccat gaaggatggc gtggacgccg cgtcgtctgg tttaacgttg cgggcgcggt
     5401 aattctgaca ctggcctact tcggcagccg aattgtccag cagttaatca gctaaaccca
     5461 gaaaaggagt ttcccctgga acacatttct actactacgt tgatcattat tctgatcatc
     5521 atggtggtca tttcagccta tttttccggg tccgaaaccg gaatgatgac cctcaaccgc
     5581 tatcgtctgc gacatatggc gaaacagggt aatcgctcgg ccaaacgcgt cgaaaaattg
     5641 ctgcgtaagc cagaccgcct gataagcctg gtgttaatcg gcaataacct ggtcaatatt
     5701 cttgcctccg cgctcggcac tattgttggg atgcgtttgt acggcgatgc gggcgtggca
     5761 attgcgactg gtgtgctgac ttttgtcgta ctggtatttg ctgaggtatt gccgaaaacc
     5821 attgccgcgc tgtacccgga aaaagtcgct tatccgagta gttttctgct ggctccgctg
     5881 caaattttga tgatgccgct ggtctggttg ctgaatgcta tcacccgtat gctgatgcgc
     5941 atgatgggta tcaaaaccga tatcgtggtt agcggctctt tgagcaaaga agagttgcgc
     6001 actatcgtgc acgaatcacg ctcacaaatt tcccgtcgca atcaggatat gctgctgtcg
     6061 gtgctcgatc tggaaaaaat gaccgttgat gacatcatgg tgccgcgcag tgaaattatc
     6121 ggtattgata tcaacgatga ctggaaatcg attctgcgcc aactctccca ctcacctcac
     6181 gggcgcatcg tgctctaccg tgattcgctg gacgacgcca tcagtatgct gcgagtacgt
     6241 gaagcctggc ggttgatgtc ggagaaaaaa gagttcacca aagaaaccat gctgcgcgcc
     6301 gcggacgaga tctattttgt gccggaaggt acgccgctca gcacgcagtt ggtaaagttt
     6361 cagcgcaaca aaaagaaagt cggcctggtc gtcaacgagt atggagacat tcaggggctg
     6421 gtgacggttg aagatattct ggaagagatt gtcggcgatt tcactacgtc gatgtcgcca
     6481 acacttgccg aagaggtcac gccgcaaaac gacggttcgg taattatcga tggcaccgcc
     6541 aacgtgcggg aaatcaacaa agcctttaac tggcatctac cggaagatga tgcccgcacg
     6601 gttaatggcg tcattcttga ggcactggaa gagatccctg tcgcaggcac ccgcgtgcgt
     6661 attggcgagt acgatataga tattctcgac gtacaggaca atatgattaa gcaggtaaaa
     6721 gtttttcctg tgaaaccgct gcgcgagagt gtggcggagt aacgaaaacg gccctgcatt
     6781 cgcatgcagg gccgtgaatt attacgaaaa cagaaattac gcttttgctt tcgctacagt
     6841 taccatcgcc gcacgaatcg tacgaccatt cagcgtataa cccttctgca taatgcccag
     6901 tacgttacct ggcgcaacgt catcagattc caccattgcg atagcctgat gcacattcgg
     6961 gtccagcggc acgttagttt cggcgatcac ttcaacacca aacttacgca caacatccag
     7021 catcgacttc agcgtcagct caatgccttc aaccatcgca gacatatccg ggttagcttt
     7081 atcagccact tccagcgcac gatccaggct atcaatcacc ggcagcaatt cgttgatgaa
     7141 tttctccagc gcgaatttgt gggctttttc aatatccagt tcagtacgtc gacgcaggtt
     7201 ttccatttcg gcttttacac gcaaaatgcc gtcacgttca cgggtctggg cttcagccag
     7261 ctgagcttcg agattcgcaa ttttttcatc gcgcggatcc acctgctcag cagaagcttc
     7321 tggctcaact gcctcaatct cttcgtgctg atccatgata atttcttccg gggcttgccc
     7381 ctcaggcgtt ttctgttctt tactactcat gaatttctcc gcgttttttt cgcattcatc
     7441 tcgctaactt cgcttattat ggggatcagt ttcagggttt caagggaagc actcacattg
     7501 tcatcaatct tcgcaacaag gacctcggaa aaatgaataa tcatttcaag tgtattggca
     7561 ttgtgggaca cccacggcac cccactgcac tgacaacaca tgaaatgctc taccgctggc
     7621 tgtgcacaaa aggttacgag gtcatcgttg agcaacaaat cgctcacgaa ctgcaactga
     7681 agaatgtgaa aactggcacg ctcgcggaga ttgggcaact agctgatctc gcggtagtcg
     7741 ttggtggcga cggtaatatg ctgggcgcgg cacgcacact cgcccgttac gatattaaag
     7801 ttattggaat caaccgtggc aacctgggtt tcctgactga ccttgacccc gataacgccc
     7861 agcaacagtt agccgatgtg ctggaaggcc actacatcag cgagaaacgt tttttgctgg
     7921 aagcgcaagt ctgtcagcaa gattgccaga aacgcatcag caccgcgata aatgaagtgg
     7981 tgcttcatcc aggcaaagtg gcacatatga ttgagttcga agtgtatatc gacgagatct
     8041 ttgcgttttc tcagcgatct gatggactaa ttatttcgac gccaacaggc tccaccgcct
     8101 attccctctc tgcaggcggt cctattctga ccccctctct ggatgcgatt accctggtgc
     8161 ccatgttccc gcatacgttg tcagcacgac cactggtcat aaacagcagc agcacgatcc
     8221 gtctgcgttt ttcgcatcgc cgtaacgacc tggaaatcag ttgcgacagc cagatagcac
     8281 tgccgattca ggaaggtgaa gatgtcctga ttcgtcgctg tgattaccat ctgaatctga
     8341 ttcatccgaa agattacagt tatttcaaca cattaagcac caagctcggc tggtcaaaaa
     8401 aattattcta attttacgcc agcctcttta ctgtatataa aaccagttta tactgtacac
     8461 aataacagta atggtttttc atacaggaaa acgactatgt tggcacaact gaccatcagc
     8521 aactttgcta tcgttcgtga gcttgagatt gattttcata gcggcatgac cgtaataact
     8581 ggcgagaccg gcgcgggtaa atctattgca atagatgccc tcggtctttg tctcggtggt
     8641 cgcgctgaag ccgacatggt gcgtaccggc gctgctcgcg ctgacctgtg cgcccgtttt
     8701 tctctgaaag atacgccagc ggctctgcgc tggctggaag aaaatcagct tgaagacggg
     8761 catgaatgtt tgcttcgtcg cgtgatcagc agcgatggtc gctcccgtgg tttcatcaac
     8821 ggtacagctg ttcctctgtc acaactgcgc gaactgggtc agttgctgat tcagatccat
     8881 ggtcagcacg ctcatcaatt actcaccaaa cctgagcacc aaaaattcct gcttgatggc
     8941 tatgccaatg aaacctctct actgcaggaa atgaccgcac gttatcagtt gtggcatcaa
     9001 agctgccgtg acctcgcgca tcatcaacag ttaagtcagg aacgcgccgc ccgtgcggaa
     9061 ctgctgcaat accaattaaa agaacttaac gaatttaatc cgcagcccgg agagtttgaa
     9121 caaatcgacg aagagtacaa acgtctggcg aacagcggtc aattgctgac caccagccag
     9181 aatgcattgg cattaatggc ctacggtgaa gacgcaaacc tgcaaagtca gctttacacg
     9241 gctaaacaac tggtgagcga attgattggc atggacagca aactgtccgg cgtacttgat
     9301 atgctggaag aagctaccat ccagattgct gaagccagcg atgaactgcg ccactactgc
     9361 gatcgtctgg atctcgatcc caaccgacta tttgaacttg aacagcgcat ctcaaaacag
     9421 atttcgctgg cacgtaaaca tcacgtcagc cctgaggcat tgccacagta ttaccagtcg
     9481 ctactggaag aacagcagca actggacgat caggccgact cacaagaaac gcttgcgctg
     9541 gcggtaacga aacatcatca gcaggcactg gaaatcgcgc gcgcattaca ccaacaacgc
     9601 cagcaatatg cagaagaact tgcacagctg atcaccgaca gtatgcatgc gctctcaatg
     9661 ccgcatgggc agtttacgat cgatgttaaa tttgacgagc atcacctggg cgctgacggt
     9721 gccgatcgta ttgagtttcg ggtaaccacc aacccaggtc agccaatgca gcctattgcc
     9781 aaagtcgcat ccggtggtga attgtcccgc atcgcactgg caacccaggt catcacggcg
     9841 cgtaaaatgg aaaccccggc actgattttt gatgaagtgg atgtagggat tagcggtcca
     9901 acagcggcag ttgtcggcaa actgctgcgt caactcggcg aatcaactca ggtgatgtgt
     9961 gttacccacc tgccacaagt cgcgggatgt ggtcatcaac actattttgt cagcaaagaa
    10021 accgatggtg cgatgacaga aacgcatatg caatccctga ataaaaaagc gcggttacaa
    10081 gagctggcgc gcctgcttgg tggcagtgaa gtcacacgta atacactggc gaatgcgaaa
    10141 gaactgcttg cagcgtaaac ttttttcctg cttcacggtc agagtaaaca gcaaaacgcc
    10201 gtaagaccgg aaagcaaaag gttttaaagt gatgaaaggt ctattatcat cggcatatta
    10261 cagatgagcc acgtactgct cgggcccgaa aaggaatcaa atcactatgc gctgtaaaac
    10321 gctgactgct gcagcagcag tactattgat gttgaccgca ggctgttcca ctctggagcg
    10381 agtggtttac cgtcctgaca tcaaccaggg gaactatctg accgctaacg acgtatccaa
    10441 aatacgtgtt ggcatgacgc aacaacaagt tgcgtacgca ttgggtacac cgctgatgtc
    10501 cgatccattt ggtacgaata cctggttcta tgtcttccgc cagcaaccag gtcatgaagg
    10561 tgtaacgcag caaacgctga cgctgacctt taacagtagc ggtgtgttga ccaatattga
    10621 taacaaacct gcgctgagtg gtaactaata ataaagttgc tctcaaagac gttaaaaaag
    10681 gtgctcaatg agcacctttt ttctgtctgt tatttattcg ctgatttttc tgctcgttgc
    10741 ctgcgaagct ctttcggatc ggcaatgaga gggcgataaa tctccacccg atcgccatca
    10801 tgcacactat cgcttagttt tgccggacgg ctgtaaatgc cgactttgtt tttagttaaa
    10861 tcgatatcgg tacgcaattc cagcaagcca ctggcgcgaa tagcttcttc aaccgtcgcg
    10921 ccctcctgca gcgtcacgcg ctgcaggtac tgcttctcag gtagcgcata agccacctca
    10981 acggcaattt tacctggcac tgtaaacctc tttcgcacga accgtaaaag cctggaccat
    11041 attagacgcc agctctttaa acacgcgacc aaaggcgagt tcaatcaact tattggtaaa
    11101 ctcaaagtcg agatgaaact cgatacgaca cgcctcctgg ctcagcggcg taaacttcca
    11161 tccaccaatc aatttcttga acggcccatc caccagattc ataagaatac tttggttact
    11221 ggtcaactgg ttgcgggtag taaacgtttt gctgatccca gccttagaga catctaccgc
    11281 cgcagtcatc tgcccaggag tggactccag aatccgactt ccggtacaac ccggcaaaaa
    11341 ctgaggataa gactgaacgt cattcactaa ctgatacatt tgctccgcgc tgtagggtac
    11401 cagtgcggtc cggctaatct gaggcataac tatttccatc aacaaaaatc caacaaataa
    11461 tatcatttat cctgctgtaa aaaaaacgct atcccggcgc tgggtaacat cgggttcatg
    11521 ctaagataga gccttgtccc ccgcaggatt gatatggggt gttttcgatt tcagattacc
    11581 gatgattcac gacgcttatg acgaagaaaa aagcacataa acctggttca gcgaccatcg
    11641 cgcttaacaa gcgcgcccgt cacgaatact ttatcggaga agagttcgaa gcgggacttg
    11701 ccctgcaagg ctgggaagtt aaatccctgc gcgcaggaaa agccaatatc agcgacagct
    11761 acgtccttct gcgtgacgga gaggcatttc tgtttggcgc taacatcacg ccaatggccg
    11821 tggcctccac gcatgtggtg tgcgatccta cccgtacccg caagttactt ctcaaccagc
    11881 gcgaactgga ctcattgtac ggtcgcgtca atcgagaagg ctataccgta gtggcgctct
    11941 ccctgtactg gaaaaatgcc tggtgcaaag tgaaaatcgg cgtcgccaaa ggtaaaaaac
    12001 agcacgataa acgttcagat atcaaagaac gcgaatggca ggtggataaa gcacgtatca
    12061 tgaaaaacgc ccaccgttaa acctgcactc caattattga ccagttcctc accgcgcctc
    12121 cctctccggc ggcgcgaatg aacatcttat tggctatcac atccgacaca aatgttgcca
    12181 tcccattgct taatcgaata aaaatcaggc tacatgggtg ctaaatcttt aacgataacg
    12241 ccattgaggc tggtcatggc gctcataaat ctggtatact tgccgttaca cattggggct
    12301 gattctggat tcgacgggat ttgcgaaacc caaggtgcat gccgaggggc ggttggcctc
    12361 gtaaaaagcc gcaaaaaata gtcgcaaacg acgaaaacta cgctttagca gcttaataac
    12421 ctgcttagag ccctctctcc ctagcctccg ctcttaggac ggggatcaag agaggtcaaa
    12481 cccaaaagag atcgcgtgga agccctgcct ggggttgaag cgttaaaact taatcaggct
    12541 agtttgttag tggcgtgtcc gtccgcagct ggcaagcgaa tgtaaagact gactaagcat
    12601 gtagtaccga ggacgtagga atttcggacg cgggttcaac tcccgccagc tccaccaatc
    12661 atgattggac ggtataagga caacaccaat aaaaacagga agttagcagt ctcagcagga
    12721 caccgaccag acggtgaaga gacataaaag gatacgcaaa ggagccgcgg ctcctggtga
    12781 catgaaagcc cacagatgtg ggctttttcg ttgatggtca gaacgaccag ttcacaccag
    12841 ctaccccgtt ccacggggat tccacaccgg caccatggct ataccccacc ccaagatgcc
    12901 cgcttaacct actgctgaat gaggctttaa tacctgcctg gtatattcca cgtctgcccg
    12961 acaaatcatt gacgaaatta ccgtcactat tcactttcac ccggttatca tcgacaaatt
    13021 ctttgcgcac agccgccttc agccacggct caacttccat accgttcccc agacgcatgt
    13081 tgtaactcag cgttgcaccc agttcacgat ataaactgcg ggtatcgact gatttcgatt
    13141 ccatgccatt ggataaatga tattcagggt tatcagcggt gaacccccgt taacgatgca
    13201 tacggcgtca ggttccagtt accatcggta aatcgcatcc cggtttcaat gtgaccgccc
    13261 agcccgttgc tgcgataact gccgttggcg gctccaccgc tgccaaccgt ggagtcgcga
    13321 gcttcggtac ctgctaatac caataaatta ccgccatttt ccagcaacat attggtcgct
    13381 aaattgccgg aaatggaaaa agtgccgtac tggtgagtac cgctgatttc aataccgtta
    13441 gccgtgctcg tctggagagc ggcaccgctg ttctggacgg tatctgtcgc tttgccatta
    13501 tcgttaactg tcagcgtacc gccttcattg atctttgttt ttattgcctc tccgttagct
    13561 gaaactgttt gtattccgcc gtcgttaatc gttgtctcat tcgccacccc ctcgacgatt
    13621 tgttcgccgc cggtgagcgt cgtgcctgtc gcagtggctt ttgttttgac gatctcccgt
    13681 ccgcccgtat tgacctgtgt tttgtcagaa gaggtgtctg actccacggt taacacgccg
    13741 ccatttgcca gcaggatatt gttcgccgca ccctgctcga tgctgaacgc gacgccatcc
    13801 gcgcgtgttc ctgtgacccg cgtcgccctg gtagttgcaa ccaaagcgcc ctggctactc
    13861 tgctgtatcc ccgttgcgct gcctttctcc cgcacatcga gtgtgccgcc gtcattaagc
    13921 accgagtttt cagccagaca gccctcatta actacctgtg ccccccccat taataatgga
    13981 accttccgct gtcccgtttg ccataa
//
LOCUS       DAJTMK010000105        13959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTMK010000105 DAJTMK010000000
VERSION     DAJTMK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13959
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.106"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            530..958
                     /locus_tag="OO135_002678"
     CDS             530..958
                     /locus_tag="OO135_002678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398478.1"
                     /translation="MAFDFYRINRFGRWEKYYNIQLRGALLSAINHLFTENNLDTEAI
                     TVSYEYILSRHLIANTEFSYLAFPDNYNRLFIPQPKTKAGNGSKHLIVKRLADCLLLA
                     AYTMGISKDSGKQQTSWGEMHLLVTINLWITKGYSSLVLQ"
     gene            883..1425
                     /locus_tag="OO135_002679"
     CDS             883..1425
                     /locus_tag="OO135_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HCR5398479.1"
                     /translation="MGGDAPAGYDQFMDNKGLLIAGASIVAGAVLGRMRLPDLESLEH
                     FGARGAVSGRPFNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMIYR
                     LKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTATLED
                     YKLKGELNDLYHQDALDYDN"
     gene            1415..1660
                     /locus_tag="OO135_002680"
     CDS             1415..1660
                     /locus_tag="OO135_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5398480.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(1945..4206)
                     /gene="metE"
                     /locus_tag="OO135_002681"
     CDS             complement(1945..4206)
                     /gene="metE"
                     /locus_tag="OO135_002681"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5398481.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREKLLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4442..5395
                     /gene="metR"
                     /locus_tag="OO135_002682"
     CDS             4442..5395
                     /gene="metR"
                     /locus_tag="OO135_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5398482.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5283..6182)
                     /gene="bioP"
                     /locus_tag="OO135_002683"
     CDS             complement(5283..6182)
                     /gene="bioP"
                     /locus_tag="OO135_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5398483.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAYLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6258..7058)
                     /gene="yigL"
                     /locus_tag="OO135_002684"
     CDS             complement(6258..7058)
                     /gene="yigL"
                     /locus_tag="OO135_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5398484.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(7066..8088)
                     /gene="pldB"
                     /locus_tag="OO135_002685"
     CDS             complement(7066..8088)
                     /gene="pldB"
                     /locus_tag="OO135_002685"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5398485.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEVHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8199..8819
                     /gene="rhtB"
                     /locus_tag="OO135_002686"
                     /pseudo
     CDS             8199..8819
                     /gene="rhtB"
                     /locus_tag="OO135_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
     gene            complement(8881..9501)
                     /gene="rhtC"
                     /locus_tag="OO135_002687"
     CDS             complement(8881..9501)
                     /gene="rhtC"
                     /locus_tag="OO135_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5398486.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9565..11394)
                     /gene="recQ"
                     /locus_tag="OO135_002688"
     CDS             complement(9565..11394)
                     /gene="recQ"
                     /locus_tag="OO135_002688"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5398487.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11527..12396)
                     /gene="pldA"
                     /locus_tag="OO135_002689"
     CDS             complement(11527..12396)
                     /gene="pldA"
                     /locus_tag="OO135_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5398488.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12561..13028
                     /locus_tag="OO135_002690"
     CDS             12561..13028
                     /locus_tag="OO135_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5398489.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTTTSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            13080..13959
                     /gene="rarD"
                     /locus_tag="OO135_002691"
                     /pseudo
     CDS             13080..13959
                     /gene="rarD"
                     /locus_tag="OO135_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
BASE COUNT         3497 a         3640 c         3465 g         3357 t
ORIGIN      
        1 gattacgact ccgctatttg accttaagaa agtcggcaag tcatagtcat aaatgagtct
       61 cttcaatgga agacacctta gagatttacc atctactccc attcacaaac aaacattaat
      121 tattatcaac acattcacat taattataaa tcatctcaga ccttcctgat aaaaagatat
      181 taaagtgaaa caatttgttt ttcacatttt agttcatcac ttttaggcgt aaataaatgt
      241 aaaaaaaaca acaatactat ttctctttca caaaagaatt aacaaaaggt tttttaagga
      301 tgagtgatat tatttacttg accataaccg gagaacaaca aggatgtatt tcctctcttt
      361 gtggaacgag cgcatcaata ggtaatcgct ggcagattgg ccatgaagat gaaatcttcg
      421 cgttctcgct ctctaatagt attacgaatg caggcaaagg gtcacaattg catggcctta
      481 gtttctgtaa attcttatta acgctattaa caataacgag cagttgttca tggcgtttga
      541 cttctatcgg atcaatagat ttggtcgatg ggagaaatat tacaacattc aactccgcgg
      601 cgcgctctta tcagcaatca atcacctgtt tactgagaac aatctggata cagaagccat
      661 cactgtcagt tatgaatata tactctccag gcatctgatt gctaatactg aatttagtta
      721 tttggcattt cccgataact acaaccgtct gtttatacca caaccaaaaa ctaaagctgg
      781 taatggttca aaacacttaa tagtaaagcg gttggcagac tgcttgctgc tggcggcata
      841 tacaatggga atatcgaagg attcagggaa acagcagaca agttgggggg agatgcacct
      901 gctggttacg atcaatttat ggataacaaa gggttactca tcgctggtgc ttcaatagtt
      961 gctggagcag ttctggggag aatgagactc cctgatttgg aatcacttga gcatttcgga
     1021 gccagaggag cagtatcagg aagaccattt aatcccgacc ttgccggtgg tccaatagaa
     1081 aatctgacga cagatggcgt aacaatcaac agggaaggta tcgctattgt tgaaaaacac
     1141 atagccagat ttggtcatga ccctgtaaat gaagtgatga tctatagact gaaagacatt
     1201 gagaaaggaa aaatcccccc agaacaagta gatcttaact tctatactca tgagtgtcgg
     1261 gaatatcagc gttactgtaa tcttggttgg gaaacaggac aacctgatgg tgacgcaggc
     1321 tatgcactct ggaatcatac gcatactgct actctagaag attataaact taagggagaa
     1381 cttaatgact tataccacca ggatgcactt gactatgata actgaaactg aaattaacgt
     1441 actgaaagtt atggctgaga aagatataaa ctggaactgg atgaatctcg atcgcacact
     1501 atccataagg caaattccag gattcggtaa tgttgttaac attgttaaca aattagctaa
     1561 cgagggtttg gttattattg aggatagcgg acataagtct atgcctcact accacgttac
     1621 tgaaaaggga tacaatttcg tgaaaagtga gaataaataa cgtagcgttg attactgaac
     1681 aaatcgctca ggattatatt ttgcgtaaaa atgattcagt tatttctgta tctgaacggg
     1741 cgcagatagc aaaatggctt ccggtgatgg aagccatcag attattggca aagtgcgcgt
     1801 tgttcatgcc ggatgcggcg tgaacgcctt gtccggccaa caaaactcaa aaattcaata
     1861 aattgcataa attatgtagg cctggtatca ggcagttttg cctctttcat cgtactgcaa
     1921 tttacagtga ctcgttaata ttcatcatcc ccgacgcaaa ttctgcgccg cctgcaccat
     1981 gttcgccagt gccgcgcggg tttctggcca gccgcgcgtt ttcaggccac agtccgggtt
     2041 gacccacagg cgctctgccg gaatgcgttt tgccgctttc ttcagcaagg cttcaatcca
     2101 ttctacgctc ggtacgtttg gcgagtgaat gtcatagacg cctggaccga tttcattcgg
     2161 ataatcaaac tcttcaaacg actccagcaa ctccatgtcg gaacgcgagg tttcgatggt
     2221 gatgacgtct gcatccagtg ccgcaatgga atccatgatg tcgttgaact cgcaataaca
     2281 catgtgagtg tggatttgtg tgtcatcttt cgccacggcg gcgttgatac ggaaggcctc
     2341 cacgccccac tggagatatg catcccagtc gctacgacgc agcggtaaac cttcgcgcag
     2401 cgccggttca tcaatctgga tgatgccaat tcctgcggct tccaggtccg ccacttcatc
     2461 acgcagcgcc agcgcaattt gtttggcgat ggtttcacgg ctgacatctt cacgcgggaa
     2521 cgaccagcag agaatggtca ccgggccagt cagcatccct ttcaccggtt tatcagtcag
     2581 agattgcgca tacttcgccc actccacggt aatcggtgcc gggcgactaa cgtcaccaat
     2641 aacaatcggt ggcttcacgc agcgggaacc gtagctctgt acccaaccgt tttgcgtaaa
     2701 gacaaatccg tcgaggtgct cgccaaagta ttccaccatg tcattacgct cggcctcgcc
     2761 atgtaccagc acatccagtc ccaaacgttc ctgctcaaca atggcctgct tgatatgttc
     2821 cgcaatgccc gtgcggtagt tgttggcgtc gagattgccc tttttgaaat ccagacgcag
     2881 ggtacgaatt tccgtggttt gcgggaagga accaatcgtg gtggtcggcc acgctggcag
     2941 tttaaaacgc gcacgctggg cttcagcacg cacttcatag acattcgcac gctggctgtc
     3001 ctgggcggtg atcgccgcca gacgcttttc taccgccgga ttatgtacac gagtagagtg
     3061 acgacgcgcc tgaattgggg cgctccactc tgccagagct gccgtgtcac cactgttcag
     3121 cgcatcgcgc agcagtgcca gttcatggca tttttgtaag gcgaaggcaa accagctttt
     3181 cacttctgca tcaagacgcg tttccacgct caggtcgatg gggctgtgca gcaacgagca
     3241 ggaagatgcc acccacaaat cacgtttgcc gacaatgtcc ttaatttgcg catatttctc
     3301 agtaagatcg gcgcgccaga cgttacgacc attgatcagt cccgcagaca gcagccagtc
     3361 agaaggcagg cgcttgtgca gttcagcaac gccatcttta ccatgtacaa ggtccacatg
     3421 cagtccctga acaggcagcg cagtaatcgt gtcgagattt ggcgtaacgc cttcaaaata
     3481 ggtagtcagc agcagtttca cctgtccctg gagcgcgtcg taagcgggtt tgtatgcgtc
     3541 cagccacgcc tgtggtagtt ccagtaccag cgcgggttca tcaatctgta cccactcgat
     3601 gccgcgtttc tccagttctg ccagcacttg ctgataaacc ggcagaatgt cgttcagcag
     3661 gctcaggcgg tcaaactgtt cacctttcac tttccccagc cacagccagg taaccggccc
     3721 cagcagcaca ggcttaacgt tgtggcccag cgccagcgcc tcgtccactt cctccagcag
     3781 ctgcgtccag gtcagtttga actgttggcc tttaacgaat tccggcacca tgtagtgata
     3841 gttggtgtta aaccatttgg tcatttccgc tgccgccgca ggttcgccag tcggcgcacg
     3901 tccacgacca atacggaaca gggtgtcaat atctaccgaa ccatctttgt tctgatgacg
     3961 cgccggaacg ttacccagca gcagactggt ggtcagtaca tgatcgtacc aggcaaaatc
     4021 gcccaccggc agcaggtcga tacccgcttg cttttgttga tcccagtgac gagcacgcaa
     4081 ttcacgccct accgccagca gtttttcacg cgtggagttc cctgcccaat aactctcttg
     4141 cgcttttttc agctcgcgac gcaggccaac gcgagggaaa ccgagggtgt gattcaatat
     4201 tgtcattttt cttcctctaa ttatatgtaa atcctatgga ttttgaattt agggaaggcg
     4261 gcaagtttat tcatccccgg gagcttactg aagtaagtga ccggggtgga gaaacgcagc
     4321 caacgcacca taaatttaaa agacgaagga ttttagccgt ccagatgttt acacatccat
     4381 aattggcggt tactgtatat tcctcaagcg caaattattc atgccgaagt gaaggacttt
     4441 catgatcgaa gtaaaacatc tgaaaacgct acaagcgttg cggaactgcg gctcactcgc
     4501 agccgctgcg gcgacgttgc atcagacgca atccgccctg tctcaccagt ttagcgatct
     4561 ggaacaacgc cttggcttcc ggctatttgt gcgtaaaagc cagccgcttc gctttacacc
     4621 gcagggagaa atcctgttgc aactggcaaa ccaggtgctg ccacaaatta gccaggcact
     4681 gcaagcctgc aatgaaccgc agcagacgcg tctgcgcatt gccattgagt gccatagctg
     4741 tatacagtgg ctgacgcccg cgttagaaaa tttccataag aactggccgc aggtcgagat
     4801 ggactttaaa tcgggcgtga catttgaccc gcagcccgcc ttgcaacagg gagagctgga
     4861 tctggtaatg acgtccgata ttctgccgcg cagtggtctg cattattcgc cgatgttcga
     4921 ctatgaagtg cgtctggtgt tagcacctga ccatccactg gcggcaaaaa cgcgtattac
     4981 accggaagat ctcgccagcg agacgctatt aatttatccg gtgcagcgta gtcgactgga
     5041 tgtctggcgg cattttcttc agccggcagg cgtcagtccg tcactgaaaa gcgtcgataa
     5101 caccttattg ttgattcaga tggttgccgc gcggatgggt attgccgcgc taccgcattg
     5161 ggtagtagag agttttgagc gccagggtct ggtggtgacc aaaaccctgg gcgaaggctt
     5221 gtggagccga ctgtacgccg ccgtgcgcga tggcgagcag cgtcagccag ttacggaagc
     5281 gtttattcgc tcagcgcgca atcacgcctg cgatcatctg ccgtttgtga agagcgcgga
     5341 gcgacccact tacgatgcac ccacagtgag gccaggatca ccagcgcgcc tgtaataaac
     5401 gttggccagt gcggctgttg gtgccagata gccaggttta ccagcagccc tgccggaacg
     5461 tgcatattat tcataattcc cagcgttccg gcgtccacct gcgtcgcgcc gtagttccac
     5521 ataaagtagc caatcccgga agccaccacg ccaagaaaca ccagaatgcc ccattgcaac
     5581 gtggtttgcg gcattttctg cgcatttccc agcaagaacc atgcaattac cgccaccagg
     5641 tacgcgccaa gatagaacca cgcaaaggcg ttatgctgcg gcatcgggcg agtttccatc
     5701 aggcgtttgt aacccaccat gccgatggca aaagtgatat tggagagttg caccagcaac
     5761 aagccagtcc agaaatggtc ggtgacctga tcatagcgaa taatcccggc accaatcacc
     5821 gccaacaagg cactaaaagc atatccccag cgcagacggc gcttattcat gatgtcataa
     5881 atcagcgtga tgtagagcgg cgtcagtacg gtgaacagca gcagttcgga aaccgtcaga
     5941 tagagataag cgcggaaact cagcatatac atcacgccga gctgcatcgc gcccaccagc
     6001 atatacaggc cgactgtttt caggctattg ccacgggtac gcagaaacgg cagaaaaacg
     6061 agtgccgcca ggccaacgcg caccagcacc gcaaaatagc tatcgacgtg ccccgcaagg
     6121 tactcgccat aaaagctaaa ggagaaggcc cacagaatcg tggtgatgat aagtagcgcc
     6181 acaatggaaa actctttgat taacgagtat ccattgtagc gaagtatcag gttgacaact
     6241 gaccaaataa agaacgatta cgataaatag agtttacgca gataatgcgg caccgcgtcg
     6301 tcggcattag taccaatcac ttccagctcg ggatgaaggt ctttcagacg ctggtgcgca
     6361 ctgcccataa tgcagccttt ccccgccatc gacagcattt cggcgtcgtt catcccgtca
     6421 ccaaacgcaa tacaatcctt caggctgtag cccagtttct tcgccaccgc ttccagcgca
     6481 tggccttttg aaacgccgcc cgccatcact tccagacagg ttaaggtaga gaaactgacg
     6541 ttgacgcgat cgccccaacg agcgttaatc gcctgctcca gcggcagcag ttgttcatgg
     6601 gaatcgcagg tgaagaacac tttgctgacg ccttccggct ccagtaatcc aggctcatac
     6661 agcgcatatt tgaacaccgc ttctttaaaa aagcgcatct cttccgggcg atggcgattc
     6721 ataaaccatt cgtcgtcgcg ataaacgtta gtaatgatgt ccggattgtc gttgactacg
     6781 ccaaacagat cgctggcaat gtcgcgatcc aggttatgag caaaaatcag attaccatcc
     6841 agatcgtgaa cgcgcgcacc attggaggta atcatgtaag acttaatctc cagattatcg
     6901 cgaatttgcc ccacatcaac gtggtgacga ccggtcgcaa acacaaagtt gatgccgcgc
     6961 gcggtgagca gcttcagagt ttctttggcg taaggggata acgtatggtc gggagaaagt
     7021 aacgtgccat ctaaatcaga cgcaacaacc tggtacataa gaaatttaaa cctctgtaga
     7081 gcggtttccg ctgggtgagt tatgcctgtt gaaaaaatca acgatggcgt ggagcgcgac
     7141 tgagcgcatt gcgtcctttt caaaaaggat ctcatggtaa gcacctttaa ttaccaacgg
     7201 ccgtcctcct tcgacaggat ggcccgcggc ggtgcggagt tcacaaaaac ggtcatgcat
     7261 gcggttatcc accacgcgtt cctcttcagc ctgcaagaga agcgttggcg tggcgtcatc
     7321 acccgcaccg gctaacacct gttcgccagc cagaatactt tcgcgtaccc aatggtaggt
     7381 cggcccaccg acgcgaatcg ttgggtcatc agcatagaag cgtaagttac gtcgatatcg
     7441 ctgtctgctg tgggtcagta cgttgatagc aaacggcaac gcgcgccagc gcccggtgcc
     7501 tattgcatag ccatcacgga aacgtggatg cacttcggcc cagttgagga tctgccgtgc
     7561 cataaatgac ggcatacgaa tcacgatccc aaacattggc gcagttagcg caatggcgtc
     7621 acatacacct ggatggcgtt gcagaaataa tgtggagatc gcaccgccca tcgaatgtgc
     7681 cagtatatag cgtttacgcc acggaccggg ctggacctcc tgctgccaga atgccgccag
     7741 atcatcaaca taatcattaa agcgattaac atgcccgaga tgcggatcgg ctaacaggcg
     7801 accggaacgt ccctgcccgc gatggtcgat gattaagaca tcaaacccca aatggaacag
     7861 gtcataggcc agttccgcat attttacgta gctctcaata cgccccgggc agatgactac
     7921 cacccggtca tggtgctgtg cgcgaaaacg gacaaagcgc accggaatgt catccacacc
     7981 agtaaactct gcttcatcac gctgacgcca gaaatcagtc agcggtccca tggtaaaagc
     8041 agcaaacgcg ttttctcttg tttcccagtc tttttgctgc tgaaacatcg ggtaatctgc
     8101 ctcttaaacc acgtaaaatc gtttttttta gcgtgcctga cacaacgctg cgacagtagc
     8161 gtattgtggc acaaaaatag acacaccggg agttcatcat gaccttagaa tggtggtttg
     8221 cctacctgct gacatcgatc attttaagcc tgtcgccagg ctctggtgca atcaacacta
     8281 tgaccacctc gctcaaccac ggttatcgcg gcgcggtggc gtctattgct gggcttcaga
     8341 ccggactggc gattcatatt gtgctggttg gcgtggggtt ggggacgcta ttttcccgct
     8401 cagtgattgc gtttgaagtg ttgaagtggg caggcgcggc ttacttgatt tggctgggaa
     8461 tccagcagta gcgcgccgct ggtgcaattg accttaaatc gctggcctct actcaatcgc
     8521 gtcgacattt gttccagcgc gcagtttttg tgaatctcac caatcccaaa agtattgtgt
     8581 ttctggcggc gctatttccg caattcatca tgccacagca gccgcaactg atgcagtata
     8641 tcgtgctcgg cgtcaccact attgtagtcg atattattgt gatgatcggt tacgccaccc
     8701 ttgctcaacg gattgctcta tggattaaag gaccaaagca gatgaaggcg ctgaataaga
     8761 ttttcggctc gttgtttatg ctggtgggag cgctgttagc atcggcgagg catgcgtgaa
     8821 aaataatgtc ggatgcggcg taaacgcctt atccgactta ctctgaagac gcgtctggca
     8881 tcaccgcgaa ataatcaaat gaatgccaaa tccggcaaat aacgccccgg caaaaccatc
     8941 aatccacttc gccagacgtt gataaccacg gcgcatttgc ggcagggcaa acaggctggc
     9001 aacgacggta aaccacgcca gtgtttcgac aatgatcagc gcaaaaatgc cccagcgcgc
     9061 ggtagtgcca acgttatcac cgacaaacaa tgagaacacc gagccaaagt agataatcgc
     9121 tttcggatta gcgagattgg tcagtaaacc tttcaggaaa ctgcgcccgc ttttcgccag
     9181 ctcaatctgt ggtgcaggcg cagatacagc ctcttttttc agtgcaccgc gtagcatctg
     9241 gtaacccatc cagcagagat acaggccacc gccaaccata atcagcgtat gcagccaggc
     9301 cattttttcg ataatcaaat gcaggccaag caacgcaatc cctgcccaaa ccataacgcc
     9361 gcaggtaata cccagcacgc ccatcatcgc ttctttacgg gaacgactga cagcggtctg
     9421 agagacaaaa aagaaatcgg gaccggggct cataagcgcc acaatgtgca ccatggcgac
     9481 ggtgagaaat aacatcaaca tacatttgac tcgcggggga aacgtttacg gagtcttcat
     9541 cctggcactt ttttatgctg ctgactactc ttcgtcgtcg ccatcgacat gcgcacgaat
     9601 cagcgccata aacggtttac caaagcgttc cagcttgcgc atcccaacgc cgttaacgct
     9661 gagcatttcg ctggcggtga tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt
     9721 aaacaccacg tacggcggga cattactttc atcggcaatc gatttacgca gtttgcgtaa
     9781 tttggcgaac agtttgcgat catagttgcc gccgaacgat ttctgcatcg ctttcggttt
     9841 gagcgccacg atacgcggca cggcaagttg caaagaggat tcgccgcgca gcaccgggcg
     9901 cgcggcctct gtcagttgta gggcagaatg ctgggcaata ttttgcgtca ccaggccgag
     9961 gtgaatcagc tggcggatca cgctcaccca atgttcatgg cttttatcac ggcccatgcc
    10021 atagactttc agtttgtcat gaccatagtc gcggatacgc tggttattag caccacgaat
    10081 cacttccacc acataaccca tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc
    10141 aatctgagca tcggttgaac cgtcgtactg tttcggcgga tcgaggcaga tatcgcagtt
    10201 cccgcacggc tcttgccgcc cttcgccaaa atagttcagc aataccagac gacggcaagt
    10261 ttgcgcttcg gcaaaagcgc ccatcgcatt gagtttatga cgttcgatat cctgtaactg
    10321 cccctgcggc ttctcttcca ggcaacggcg cagccacgcc atatcggccg gatcgtaaaa
    10381 cagcatcgct tccgcgggca ggccatcacg cccggcgcga ccggtttcct gataatagga
    10441 ttcgatattg cgcggaatat caaagtggac cacgaagcga acgtttggtt tattgatgcc
    10501 catgccgaac gccaccgtcg ccaccacaat ttgcaggtca tcgcgctgaa atttttcctg
    10561 cacgtcggcg cgaacattat tttccagccc agcatgatag gccgccgcgc taattccctt
    10621 gctttgcagg cgcgcagcgg tgtcttctac tttcgcgcgg ctgttgcagt agataatgcc
    10681 tgacttaccg cgctgttcct gcacgtagcg catcaactga tcgagcggtt tgaacttctc
    10741 catcagcatg tagcgaatat tcggacggtc aaaactgctg atttgaatca gcggatcgtt
    10801 cagccccagc aagcgcacga tatcctggcg cgtggtgtcg tcggctgtgg cagtcagcgc
    10861 cataaacggc agggtcggga aacgctggcg taactgacca agcgcggcat attccgggcg
    10921 gaaatcgtgg ccccattggg agatacagtg cgcttcatca acggctaata acaccggatt
    10981 ccagtgcgcc agatgctcaa gaaagttatc cagcatcagg cgttccgggg cgatataaag
    11041 cagacgaatt tgcccggtgc ggcagcctgt catcacttca agttgctgct cgcgggtttg
    11101 cgtcgagtta aggcacgccg ccgccacgcc gttggcttgc agttgatcca cctgatcttt
    11161 catcaacgaa atcagcggtg aaacaaccac ggtaaggccg tttagcaata aggcaggaat
    11221 ttgatagcaa agggattttc cgccaccggt gggcatgacg accaggcaat cgcgaccgga
    11281 aagcacagtg tcgataattt cttcctggcc ggggcgaaac tgttggtagc caaaggtttc
    11341 ttgtaaaacc tgtttagctc cggactccag attcaacact tccgcctgcg ccacattcac
    11401 cccgattgcc tgaaataaaa acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg
    11461 cttttagtct ctgagagggt aatcctggat gctgggttcc agatttgcca accactcaac
    11521 cgttcatcaa aacaagtcgt ttagcataac tcctacaccg acacgggtct ggttgaagtt
    11581 atagtcgatg agcgattcgc catagccgct gtaaacctga gtataaaggc gcacatgttt
    11641 ggtgatcggg taacttaagc ctaactccgc gccgccgtaa ccggtattcc agttgtactg
    11701 tcctttcgca ctcaacaccg catcaccgag gtgatagccg attttaagct ggtagtaacc
    11761 catatatttg gtgatatccg ggttatcgtc agtattaccc accacatacc acggcttcac
    11821 ttctaccagc cagttaccgt tttctgccat caggcgagta taaaggcggt tccagctgcg
    11881 ggaggtcggg tcggaacgcc cgttagagtc gtggttatac cccatctcca catcgcgcag
    11941 cgtccaacct gcaaaacggt aatcggtggc aaaaccgagg aacaattgcg gttcgtagtt
    12001 ggtttcacga aacggtgaag actcttcgct attggacagt tgccaccagg atttttgcgt
    12061 ataagacgca cccaacaccg agttcgggcc taaaatccca cgccacagtg gaaatgccag
    12121 gctcaactga aactttactt catccttacg cgcattttcc gcccagtcgt aactggcaat
    12181 cgcttctttg ttcagatcgc tggtttgggt gtagatgagg tagttggtgt cataaggata
    12241 gagcgtgaac ggattgtcat gctcctgcag catattggcg ataatactgc cacgcaccgc
    12301 tggcgcgtca tgcacctctt tcaccgttgc ctcttgtgca tatactgcca taggcaacat
    12361 aaacaccggc aacaaccagc cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt
    12421 attgataagg tgaatgtttt tctgcgggct attttacata cttaactcca ttgccgttag
    12481 ttatcccttc ctaaagtaga aagcaacaac aaagaagcat aaaatcaaca tttcattaac
    12541 caatgaagtg taaggatgat atgtctgccg tactgaccgc tgaacaagcc ctgaaattag
    12601 tgggtgagat gtttgtttat cacatgccat ttaaccgcgc gttagggatg gagctggagc
    12661 gttacgaaaa agagttcgca cagctggcct ttaaaaatca gccgatgatg gtgggtaact
    12721 gggcgcaaag cattttgcac ggcggagtca ttgcgtcggc gctggatgtc gccgccggtc
    12781 tggtgtgcgt gggaagtacc ttaacccgcc acgaaaccat cagtgaagat gaactacgcc
    12841 agcggctatc gcggatgggg accattgatc ttcgcgttga ttatctgcgc ccaggcaggg
    12901 gcgagcgttt tactactacc agtagcctgt tgcgtgcagg caataaagtc gccgtcgccc
    12961 gcgttgaatt acacaatgaa gaacagcttt atattgccag tgccaccgcc acctatatgg
    13021 taggttgaag acataaaagc aggtaaactg ctgttaccaa ttaaaaatga gtattcccga
    13081 tggatgcaaa acaaacgcgg cagggcgtat tactcgctct tgccgcttat tttatttggg
    13141 gtattgcgcc agcatacttc aagctgattt actacgtgcc cgccgatgag atcctgacgc
    13201 atcgcgtgat ctggtcgttt ttctttatgg tggtgctgat gagcatttgc cgccagtggt
    13261 cctatttaaa aacgctgatt cagacgccac agaaaatttt tatgctggca gtctctgccg
    13321 tgctgattgg tggcaactgg ctactgttta tctgggcggt gaacaatcac catatgctgg
    13381 aagcgagcct tggttacttt attaacccgc tggtgaacat tgtgctgggg atgattttcc
    13441 tcggcgagcg attccgccgg atgcaatggc tggcggtgat tctggcgata tgtggcgtgt
    13501 tggttcagtt gtggactttt ggttcgctgc caatcatcgc gctggggctg gcgtttagtt
    13561 ttgccttcta cggactggta cgcaagaaaa ttgccgttga agcgcaaacc ggcatgttaa
    13621 tcgaaaccat gtggctgctg cccgtggcgg cgatttatct gtttgctatt gccgacagct
    13681 caaccagcca tatggggcaa aacccgatgt tgctgaattt gctgctgatc gccgccggta
    13741 ttgtcaccac cgtaccgctg ttgtgtttta ccgcagctgc tacacgcctg cgtctctcca
    13801 cgttaggctt cttccagtat attggcccga cgctgatgtt cctgttggca gtgacgtttt
    13861 atggtgaaaa accgggtgcc gattagatgg tgaccttcgc ctttatttgg gtggcgctgg
    13921 caatttttgt gatggatgcg atttatacgc agcgtagaa
//
LOCUS       DAJTMK010000106        13796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTMK010000106 DAJTMK010000000
VERSION     DAJTMK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13796
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.107"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            65..172
                     /locus_tag="OO135_002692"
     CDS             65..172
                     /locus_tag="OO135_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5398490.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(321..1175)
                     /gene="kdsA"
                     /locus_tag="OO135_002693"
     CDS             complement(321..1175)
                     /gene="kdsA"
                     /locus_tag="OO135_002693"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5398491.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(1211..2020)
                     /locus_tag="OO135_002694"
     CDS             complement(1211..2020)
                     /locus_tag="OO135_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5398492.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMILAQINNIAHKHIVLH"
     gene            complement(2024..2416)
                     /locus_tag="OO135_002695"
     CDS             complement(2024..2416)
                     /locus_tag="OO135_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5398493.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(2413..3246)
                     /gene="prmC"
                     /locus_tag="OO135_002696"
     CDS             complement(2413..3246)
                     /gene="prmC"
                     /locus_tag="OO135_002696"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5398494.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(3246..4328)
                     /gene="prfA"
                     /locus_tag="OO135_002697"
     CDS             complement(3246..4328)
                     /gene="prfA"
                     /locus_tag="OO135_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5398495.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(4370..5626)
                     /gene="hemA"
                     /locus_tag="OO135_002698"
     CDS             complement(4370..5626)
                     /gene="hemA"
                     /locus_tag="OO135_002698"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5398496.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            5840..6463
                     /gene="lolB"
                     /locus_tag="OO135_002699"
     CDS             5840..6463
                     /gene="lolB"
                     /locus_tag="OO135_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5398497.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            6463..7314
                     /gene="ispE"
                     /locus_tag="OO135_002700"
     CDS             6463..7314
                     /gene="ispE"
                     /locus_tag="OO135_002700"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5398498.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            7465..8412
                     /gene="prs"
                     /locus_tag="OO135_002701"
     CDS             7465..8412
                     /gene="prs"
                     /locus_tag="OO135_002701"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5398499.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPMVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            8537..10216
                     /gene="dauA"
                     /locus_tag="OO135_002702"
     CDS             8537..10216
                     /gene="dauA"
                     /locus_tag="OO135_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5398500.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(10271..10549)
                     /gene="ychH"
                     /locus_tag="OO135_002703"
     CDS             complement(10271..10549)
                     /gene="ychH"
                     /locus_tag="OO135_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5398501.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            10827..11411
                     /gene="pth"
                     /locus_tag="OO135_002704"
     CDS             10827..11411
                     /gene="pth"
                     /locus_tag="OO135_002704"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5398502.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            11528..12619
                     /gene="ychF"
                     /locus_tag="OO135_002705"
     CDS             11528..12619
                     /gene="ychF"
                     /locus_tag="OO135_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5398503.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            13388..>13796
                     /locus_tag="OO135_002706"
     CDS             13388..>13796
                     /locus_tag="OO135_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398504.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIG"
BASE COUNT         3383 a         3520 c         3423 g         3470 t
ORIGIN      
        1 aatcttgaac catcaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
       61 ctatatgacg ctcgcgaagt ttgccatgat tttctggcac gacctggcag caccgatcct
      121 ggcggggatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
      181 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
      241 tgcggccttt tttgtatctg gcacccatga aaaaagtctt aacgcagaac gctaagactt
      301 tatttttcaa gcaaaaaaaa ttacttgctg gtatccagtt cttcgaaacc tttcaccaga
      361 tcatcaatcg ctttcatctg cttgaggaac ggttccagtt tagccagcgg cagcgcggat
      421 ggaccatcac atttcgcatg ttccggatcc ggatgcgctt caataaacag ccccgccaga
      481 cctaccgcca taccggctcg tgccagctca gccacctgag cacgacgacc accggaagct
      541 gcgccaaacg gatcgcggca ttgcagtgcg tgggtcacgt cgaaaatcac cggcgagtta
      601 ccagacactt tcttcataat gctgaagccc agcatatcga caaccaggtt gtcatagccg
      661 aagttagcac cacgatcgca aagaatcact ttttcgttgc cgccttcttt gaatttatca
      721 acgatattac ccatctgtcc cgggctgaca aactgtggtt tcttgacgtt aattaccgca
      781 ccggttttcg ccatggcttc aaccaggtca gtctggcgag caagaaacgc cggcaactga
      841 atcacatcca cgacatcagc aacgggctgt gcctgacttg gttcgtgaac gtcggtgata
      901 attttcacgc caaaagtctg cttcaactcc tggaagattt tcatcccttc ttccaggccc
      961 ggtccacgat aagagtggat ggaggagcgg ttggctttgt caaaagaggc tttgaacacg
     1021 taagggatac ccagtttctg ggtcacagtt acgtagtgct cgcaaatgcg catcgccaga
     1081 tcgcgagatt ccagcacgtt cataccgcca aacagtacga acggcaggtc atttgctacg
     1141 ttgatgtcgc caatgctaac cactttttgt ttcataggat cgccttaatc ttgagtaaaa
     1201 tgtcgattaa ttaatgcagc acaatatgtt tatgcgcgat gttatttatt tgcgcaagga
     1261 tcatttcgct gatcgggtct tccggacact gttcaacgaa ataacttaaa tcgttcaacg
     1321 caacgtgttc gcaatccagt tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat
     1381 cttcagggtt gaattgtaat aaagcttcgc tggtgcgtaa cgccagctcc atctgatttt
     1441 cttccatcaa cgaggctttg agtgtatcca gcaatttgcg gattacctca atgttatcag
     1501 cttcatcaag gtcttcataa aacagttccg ccgacgggct gatatttccc tttaaccata
     1561 cgtccagcat atgttcgctt aacgattcac cgttaaaagg attaatcagc caaatttcgc
     1621 catccggaca ttcaatgcgc aatatcagct gcgtagggaa aatcaccggc agcagcggca
     1681 aatcgagacg attcgcgacc cataataaaa ccgcacctaa tgataccgca ctgccctgtc
     1741 gattctttaa cacctggtcc agccataatg catcggaaag acgataaacg ccgcgtgagg
     1801 ctttaaatcc ccagtcgccg taaaacagcg cgatcagttt ttccagctgc tcttctaaag
     1861 gcagaagctg gctgatttct tccttcgcca gactaacgag acgctccagt tcgtcgtaaa
     1921 catcttgcga gggaaaatcg cggcggattg cttcgcaagc caggatcatg ccttcgcaca
     1981 atggcgcttt attaaattcg aaatcagcta acgatctcat gacttacccc agtaacggta
     2041 cttttgtggt ggcgagttta atgatgatgt acagcaccac caacgccagc gggaaggcaa
     2101 taatgcgcgc ctgctgactg tgcatacgac gataatcgag tgcaataaaa cccaaaacga
     2161 tataaatgat aactccaaac agcttttcag tcagccactg tgcctgcccg gaaaatggca
     2221 ggatgtgcgc tttagccatc aacgcaatgc cgcttaacaa taacaccgta tcgacaaccg
     2281 gcggcacaat gcgcgtccag cgagcaaatg cctgtggatg cttctgataa cgtagccaga
     2341 aacgtaaggt taatagccca acagaaagcg cgatactaat aagatgaaca ctaagcagtg
     2401 tagaaaaact tgtcattgat aatagcggcc gagcgttacg cgctcgttat caccatagtc
     2461 acggcaggtt tcgacgtcat gataccccgc gaggataaat gcttgtcgca ccgcttcgcc
     2521 ctgctgccag ccatgttcca gaagcagaaa gccgccggat accagcgcgt tacgcgactg
     2581 ttcgatgata tgcacgatgt ctgccattcc actgtctgcc gcaaccagcg cagtgagcgg
     2641 ctcaaagcgg acatcgcctt gttgaagatg tgggtcctgc tcgtcaatat acggcggatt
     2701 gctgacaatc atcgcaaact gctgcccggc tagcgcgcta aaccagtcgc tttgcagaat
     2761 gtggatattt ttgatcgcca gatgctgggc attacgttgt gccagggaga cagcatcagg
     2821 catacgatct acagcgataa tttcgcagtc cgggcgctcg ctagccagcg caagcgcaat
     2881 cgccccggta cccgtcccga gatcaagaat acggcaaggt tgttcaggca accgcgccag
     2941 tgcctgctcc accagacact ccgtatccgg gcgcggaatt aaggtcgctg gcgaaacaaa
     3001 taacggcaac gaccagaatt ctcgcacccc ggttaaatga gcaatgggtt caccatcgcg
     3061 acgacgtgtc agtagcgcat caagttgctg acattgttcg tcagtcagct gcgtttcacc
     3121 aaaggcgaga ataaaagtac gccctctgcc ggtaacatgc tccagcagga tttcagcatc
     3181 acgccgcggg ctttcgctcg cctgaagttg gcttattgct tcacgtaacc agtgttgata
     3241 ttccattatt cctgctcgga caacgccgcc agttggtcgg cctgatgttc ctggataatc
     3301 ggttcaatca gcatatccag cttaccttcc atcacttcat ccaggcggta gagcgtcagg
     3361 ttgatgcggt gatcggtaac gcgcccctgc gggaaattgt aagtacggtt acggtcgctg
     3421 cgatcgccac tccccagcag gttacgacgg gtagacgctt cggcctgttg gcgttttgcc
     3481 atttcagcag cgtggatgcg agcaccgagc actgaaagcg ccttcgcttt gtttttatgt
     3541 tgtgaacgtt catcctgaca ttcaacaaca atcccggtcg gcaagtgagt aatacgaatt
     3601 gccgaatcgg tggtgttaac gtgctgacca cccgcccctg acgagcggaa agtatcaatg
     3661 cgtaaatctg ctgggttgat gtccggcagt tctgcgtcag gcagttctgg cattaccgca
     3721 acggtacaag cagaagtatg aatacgaccc tgcgattccg tagcaggaac acgctgcacg
     3781 cgatgaccgc cagattcaaa tttcagacga ccatacacac catcaccgct aattttggcg
     3841 atgatctctt tataaccacc atgttcaccc tcgctggcgc tcatgatttc tacccgccag
     3901 cggcgggctt cggcgtaacg gctgtacata cggaacagat cgcctgcgaa cagcgccgct
     3961 tcgtcgccgc cggttccggc tcggacttcg aggaaggcgt tacgttcgtc atcaggatct
     4021 tttggcagta acagaacctg taattgctgt tccagttgct cgcttttttc tttagctttg
     4081 cgcagttcat cctgcgccat ctcacgcatt tcaggatcat cgagcatcat ctgtgcggtt
     4141 tcgatatctt cctgaacctg ttgccagtcg gtaaaacagc gcgaaacatc acttaactgc
     4201 gcatattcgc gtgataatgc gcgaaaacgt tcctggtcgg cgatagtttg cgcgtcaccc
     4261 agcaacgcct gaacttcttc atggcgttca tgcagggctt ccagtttggc aacgatagaa
     4321 ggcttcatag gcgtaaatgc accctgtaaa aaaagaaaat gatgtactgc tactccagcc
     4381 cgaggctgtc gcgcagaata ttcaggcgtt cgttatcccc gtcacgggcg gcctgttgaa
     4441 gtgatttcgt tggcgcatgg atcaagcggt tagtcagttt ccatgccaga tcctgcataa
     4501 tggcttgcgc gtcgccgccc tgctcaaggg ccgctaacgc tttggcggtt aactcatcgc
     4561 gaacgtgctc tgcctggctg cgatactcgc gaatggtttc gctggcgctt tgtgctcgca
     4621 gccacgccat aaattcgctg gtttcctgag cgacaatagt ttccgcctca accgctgcgg
     4681 ctttacgctg cgccaggttg tgcgaaatga tgctttgcag atcgtccaca ctataaagat
     4741 aagcattcgc cagtttgcca acttccggct caacatcgcg cggaacggca atatccacca
     4801 acagcattgg ttggttacga cgacttttta atgcgcgctc caccatgcct ttcccgataa
     4861 tcggtaacgg gctggcggtg gaactgatga tgatatcggc ttcgcgcaga cgttcgtcga
     4921 tctcactcag ggcaatcact tccgcgccaa cttcatctgc cagaatttgg gcacgttcgc
     4981 gagtgcggtt ggcgataatc atcttctgta ctttatgttc gcgcagatga cgcgctacca
     5041 gctcgatggt ttcgcccgcg cctaccagca acactgtgac cgtagagagc gattcaaaga
     5101 tctgccgcgc cagcgtacaa gccgcaaaag cgacagacac agcactggca ccgatatctg
     5161 tttcagtgcg aacgcgtttc gctacagaga aagatttctg gaacatgcgt tccagttcgc
     5221 tggccttcat atgacctttt tgcgaatcgg caaacgcttt tttaacctga ccgaggatct
     5281 gcggctcccc aagaaccagt gaatccaggc cgctggcaac acgcattaaa tggctaaccg
     5341 cgtcgttatc ctgatgccag tagaggcttt tacgcagatc ttcttcatta agattgtgat
     5401 aatcgcaaag ccagcggatt aacgcctctt gcaggttatc ctgctcttca acgctaagat
     5461 aaagttccgt gcggttgcac gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa
     5521 gcaggctgtc aagcgcctga tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg
     5581 atacaggtgc cgttttatgg ttgataccga gtgctaaaag ggtcatgtct gcgggaaata
     5641 ataccaacgt tgatagggtt agtctgcttg catcatacag gatgcgtaag atcaataaaa
     5701 gagagcgccc ccttttggag taattgccga aagccgttag attctggcaa ttaagacaac
     5761 ttgaacatag acgatagcgg acggtaacgc tagcattaag ggttataact gcaacgtatc
     5821 tcaaggactt gtcatcacta tgcccctgcc cgattttcgt cttatccgcc tgctaccgct
     5881 ggctgctctt gtgctcactg cctgttccgt taccacgccc aaaggtcctg gcaaaagccc
     5941 ggattcgcca caatggcgtc agcatcagca agacgtgcgc aatcttaatc agtatcagac
     6001 tcgcggcgcg ttcgcttata tttctgacca acaaaaagtg tacgcccgct ttttctggca
     6061 gcaaaccggc caggatcgct accgtctgct gctcactaac ccattgggca gcacggaact
     6121 ggagctgaat gctcaaccgg gtaacgtgca gttagtcgac aataaaggtc agcgttatac
     6181 cgccgatgac gccgaagaga tgattggcaa attgaccgga atgccaattc cgctcaacag
     6241 cttgcgccag tggattttag gtttaccggg tgatgcaacc gactacaaac tggacgacca
     6301 gtaccgcctg agcgaaatta cctacagcca gaatggcaaa aactggaagg ttgtttatgg
     6361 tggttatgac accaaaacgc atcctgcgat gccagccaat atggaactca ccgacggtgg
     6421 tcaacgcatc aagttaaaaa tggataactg gatagtgaaa taatgcggac acagtggccc
     6481 tctccggcaa aacttaatct gtttttatac attaccggtc agcgtgcgga tggttaccac
     6541 acgctgcaaa cgctgtttca gtttcttgat tacggcgaca ccatcagcat tgagcttcgt
     6601 gacgatgggg atattcgtct gttaacgccc gttgaaggcg tggaacatga agataacctg
     6661 atcgttcgcg cagcgcgatt gttgatgaaa actgcggcag acagcgggcg tcttccgacg
     6721 ggaagcggtg cgaatatcag cattgacaag cgtttgccga tgggcggcgg tctcggcggt
     6781 ggttcatcca atgccgcgac ggtcctggtg gcattaaatc atctctggca atgcgggcta
     6841 agcatggatg agctggcgga aatggggctg acgctgggcg cagatgttcc tgtctttgtt
     6901 cgggggcatg ccgcgtttgc cgaaggcgtt ggtgaaatac taacgccggt ggatccgcca
     6961 gagaagtggt atctggtggc gcaccctggt gtaagtattc cgactccggt gatttttaaa
     7021 gatcctgaac tcccgcgcaa tacgccaaaa aggtcaatag aaacgttgct aaaatgtgaa
     7081 ttcagcaatg attgcgaggt tatcgcaaga aaacgttttc gcgaggttga tgcggtgctt
     7141 tcctggctgt tagaatacgc cccgtcgcgc ctgactggga caggggcctg tgtctttgct
     7201 gaatttgata cagagtctga agcccgccag gtgctagagc aagccccgga atggctcaat
     7261 ggctttgtgg cgaaaggcgt taatctttcc ccattgcaca gagccatgct ttaagccggg
     7321 caagctgagt ttcggtgaca acgtcacctt gttccagacg ttgcatcgcg ctctttaata
     7381 caccgcctgg aaaggatcat gcctggcccg cacagttttc ggcagattct ttccaccaat
     7441 ggacgcatgc ctgaggttct tctcgtgcct gatatgaagc tttttgctgg taacgccacc
     7501 ccggaactag cacaacgtat tgccaaccgc ctgtacactt cactcggcga cgccgctgta
     7561 ggtcgcttta gcgatggcga agtcagcgta caaattaatg aaaatgtacg cggtggtgat
     7621 attttcatca tccagtccac ttgtgcccct actaacgaca acctgatgga attagtcgtt
     7681 atggttgatg ccctgcgtcg tgcttccgca ggtcgtatca ccgctgttat cccctacttt
     7741 ggctatgcgc gccaggaccg tcgcgtccgt tccgctcgtg taccaatcac tgcgaaagtg
     7801 gttgcagact tcctctccag cgtcggtgtt gaccgtgtgc tgacagtgga tctgcacgct
     7861 gaacagattc agggtttctt cgacgttccg gttgataacg tatttggtag cccgatcctg
     7921 ctggaagaca tgctgcagct gaatctggat aacccaatgg tggtttctcc ggacatcggc
     7981 ggcgttgtgc gtgcccgcgc tatcgctaag ctgctgaacg ataccgatat ggcaatcatc
     8041 gacaaacgtc gtccgcgtgc gaacgtttcc caggtgatgc atatcatcgg tgacgttgca
     8101 ggtcgtgact gcgtactggt cgatgatatg atcgacactg gcggtacgct gtgtaaagct
     8161 gctgaagctc tgaaagaacg tggtgctaaa cgtgtatttg cgtacgcgac tcacccgatc
     8221 ttctctggca acgcggcgaa caacctgcgt aactctgtaa ttgatgaagt cgttgtctgc
     8281 gataccattc cgctgagcga tgaaatcaaa tcactgccga acgtgcgtac tctgaccctg
     8341 tcaggtatgc tggccgaagc gattcgtcgt atcagcaacg aagaatcgat ctctgccatg
     8401 ttcgaacact aatcgaaccc ggctcaaaga cccgctgcgg cgggtttttt tgtctgtaat
     8461 atccatttgt atgacctatg cctccttcac ctgccattta gttgacagat gatgcgctca
     8521 cggatgaaac attattgtga acaaaatatt ttcctcacat gtgatgcctt tccgcgctct
     8581 gatcgacgct tgctggaaag aaaaatatac tgccgcacgg tttacccgtg acctgattgc
     8641 cgggataacc gtcgggatta ttgctatccc gctggcgatg gcgttggcta ttggtagtgg
     8701 tgtggcaccc cagtacggtt tatataccgc agctgttgcg gggattgtca ttgctctgac
     8761 gggtgggtca cgctttagcg tttccggtcc gactgcggca tttgtggtaa ttctctatcc
     8821 cgtgtcgcaa cagtttggac tggcaggact gctggttgcg gccttgctgt cggggatctt
     8881 tttgattctg atgggtctgg cacgctttgg tcgcctgatt gagtatattc cggtttccgt
     8941 caccttaggt ttcacctcgg gtatcgggat caccatcggt accatgcaga ttaaagattt
     9001 tctcggtctg caaatggccc atgtcccgga acattatcta caaaaagtcg gcgcattatt
     9061 tatggcgctg ccgaccatta atgtgggtga tgctgccatt ggcattgtga cgctaggtat
     9121 tcttgttttt tggccgcgtc tgggcattcg tttacccggt caccttccgg ccttgctggc
     9181 tggttgcgcg gtgatgggga ttgttaacct gctcggcgga catgttgcta ccatcggttc
     9241 gcaattccac tacgtcctgg ccgatggttc tcagggtaac ggtattccgc aactgctgcc
     9301 gcaactggtg ctgccgtggg atctgcctaa ttcagaattc acgctaacct gggattctat
     9361 tcgcacactg ctgcctgcgg cattctcaat ggcaatgctc ggcgcaatcg aatctctgct
     9421 ctgcgccgtg gtactggatg gtatgaccgg gacgaaacac aaggcgaaca gcgaactggt
     9481 tggacaggga ctggggaata ttatcgctcc attctttggt ggtattaccg ctacagctgc
     9541 catcgcgcgt tctgccgcta acgtccgtgc cggggcaact tcccctatct cagcggtgat
     9601 ccactctatt ctggttattc ttgccctgct ggtactggca ccgctgctct cctggctgcc
     9661 gctttccgct atggcagccc tgctgttgat ggtggcgtgg aacatgagtg aagcgcataa
     9721 agtggtcgac ttgctgcgtc atgcaccgaa agatgacatc attgtcatgc tgctgtgcat
     9781 gtcgctgacc gtgctgtttg atatggttat tgccatcagc gtggggatcg tgctggcatc
     9841 gctgctgttt atgcgtcgta tcgcacgtat gactcgcctg gcaccggtag tcgtagatgt
     9901 tccagacgat gttctggtac tgcgcgttat tggcccgctg ttttttgctg ctgctgaagg
     9961 cttgttcacg gacctggagt cacgtcttga aggcaaacgg attgtgattc tgaagtggga
    10021 tgccgttccg gtacttgatg ctggtggtct tgatgcgttc cagcgttttg tgaagcgtct
    10081 gcccgaagga tgtgaactgc gcgtgtgcaa cgtggaattc cagccactgc gcactatggc
    10141 tcgcgcaggc attcaaccga tcccgggacg cctcgcgttc ttcccgaatc gtcgcgcggc
    10201 gatggcggat ttataagtac gagattgacc agtcagcgca aactgactgg tcagcaaact
    10261 gcattatttg ttagctatga cggcgattat cgctacggcg gcaacgtttg tcatagtggc
    10321 gaacccacca gtaacggtcg cacacctgtt catgcccgcc aacacgggca cccgccagcc
    10381 agagaatggc accgacgaaa atacttagca ctgcaccatg tgcgaaatac tggggcatat
    10441 taaactgtgg taactggttg aggattgaat accccacgcc gaccaccatt accaccagac
    10501 ccaaccccat gagcacgtta ccgagtaacg aagcgttttt gcgtttcata tgtcacctcc
    10561 ggaactttct gggttgtaac agggaatacc cctcttcctt atgtgtaaag tatagacaac
    10621 accctgtggg ttatgtgcgg gcgtgatcac aattacaacc cttatttcaa caaaacttta
    10681 caaataaacg cctgaacacc ttgactttct aatagtccac tcaggcaatt attacgttat
    10741 ctgcatttga tacgcagttt tttgtcaagc ggggccgcaa ccagtaaact acgcgccagt
    10801 tatgtacaca ctcaggacaa aaaaacgtga cgattaaatt gattgtcggc ctggcgaacc
    10861 ccggtgctga atacgccgca acgcgacata atgctggtgc ctggttcgtt gacttactgg
    10921 cagagcgttt gcgcgctccg ctgcgcgaag aggctaaatt ctttggttat acttcgcgag
    10981 tcactcttgg aggcgaagat gtccgcctgt tagtcccgac tacatttatg aatctcagcg
    11041 gcaaagccgt tgcggcgatg gccagttttt tccgcattaa tccggacgaa attctggtgg
    11101 cccacgacga actggatctg cctcctggcg tcgccaaatt taaattgggc ggtggccatg
    11161 gtggtcacaa tggactgaaa gacatcatca gtaaattggg taataaccct aactttcacc
    11221 gtttacgcat cggaatcggt catccgggcg ataaaaataa agttgtcggt tttgtgttag
    11281 gcaaaccgcc tgttagtgaa cagaagttaa ttgatgaagc cattgacgaa gcggcgcgtt
    11341 gtactgaaat gtggtttaca gatggcttga ccaaagcaac gaaccgattg cacgccttta
    11401 aagcgcaata agtcgttgtc tgcggcattt ttgccgagtg ccgtgtataa taggcaaagt
    11461 tatttccatt tctgcaatct gttagcaata acaggttgat tattaagata ttaaggtgat
    11521 ttaaatcatg ggattcaaat gcggtatcgt cggtttgccc aacgtcggga aatctaccct
    11581 gttcaacgcg ctgaccaaag ccggtattga agcggccaac tttccattct gcaccattga
    11641 gccgaacaca ggcgtcgtac caatgcctga tcctcgcctg gatcaactgg ctgaaatcgt
    11701 aaaaccgcag cgtacgcttc ccacgaccat ggaatttgtc gatatcgccg gtctggtaaa
    11761 aggcgcatcg aaaggcgaag gtctgggtaa ccagttcctg accaacatcc gtgaaaccga
    11821 agcgatcggt cacgttgttc gctgctttga aaatgacaac atcattcacg tttccggcaa
    11881 agttaacccg gctgacgata ttgaagttat caacaccgaa ctggcgctgg cagacctcga
    11941 cacctgcgaa cgtgcgattc atcgcgtaca gaagaaagcc aaaggtggcg ataaagacgc
    12001 gaaagctgag ctggcggtcc tggaaaaatg cctgccccag ttggaaaatg caggtatgct
    12061 gcgcgcgctg gatttaagcg ctgaagagaa agcggctatt cgttacctga gcttcctgac
    12121 gctgaaacca acaatgtaca tcgccaacgt caacgaagac ggttttgaaa acaacccata
    12181 tcttgaccag gtgcgtgaaa tcgcggcgaa agaaggttct gttgtggttc cggtttgtgc
    12241 tgctgttgaa gcagacattg ccgaactgga cgacgaagaa cgtgacgagt ttatgcagga
    12301 gcttgggctg gaagagccgg gcctgaaccg tgtgatccgt gccggttata agctgctgaa
    12361 cctgcaaact tacttcaccg ctggggtgaa agaagtgcgt gcatggacca ttccggttgg
    12421 agcaaccgcg ccgcaggcag cgggcaaaat ccatactgat tttgaaaaag gctttatccg
    12481 tgcacaaacc atctcgtttg aagatttcat cacttacaaa ggtgaacaag gcgcgaaaga
    12541 agcaggcaaa atgcgtgcag aaggtaaaga ttacatcgtg aaagatggcg atgtgatgaa
    12601 cttccttttc aacgtctaat taatttattg tctcatgagg tttcgctaaa tctcatgaag
    12661 agcgcaaaag tatataaaat ccacgcaatt gcgtggattt ttcatttcac cgagtcctaa
    12721 tcattcttgt aacatcctac acttttgtgc ttattgccta tagtgaacca cgagaataat
    12781 taaagacata agtgttcaga tgacggatat ctcattagca gtgatataac gtaagttttt
    12841 gtatcactac acatcagcca ccttcacagc tgatcaaaat tgatcacgct ccaatatgct
    12901 ggcaagatga aaatgataac cagtagcaca ataacaacga taggccagaa aacccattga
    12961 ccaaaaatga atatcgatgt tttttgcttt ttcttattag taatgcaaaa atggcgcata
    13021 ttgtttttgt tatgcagata catcaagcga aattcccata actaattagg attattacaa
    13081 aaaatcttca ccaaattttt taaccgctgt ttgttgtaat atttttgcca tatttatagt
    13141 aacgaattgg aataagtaac gacaacttag ggaataactc gatataaact cacctttgta
    13201 agggggatgt taaatatata ttaattcctg tgttcctgat tttgcccggt aatagagaat
    13261 tattctctga gaagctcatc atgattgcac catacaaata tcatgatgat aagaggatat
    13321 tattcattga tttaaacaaa atgacaaaat ccatagaccc atagtctggg tactatatag
    13381 gagtggtatg ggcatcaaac aacacaatgg gaataccaaa gcagatcgtc tcgctgaatt
    13441 aaaaatccgt tcgccctcaa ttcaactgat aaaatttggc gctattggtt tgaatgcaat
    13501 tatcttttcc cccctgctga tagctgctga tacaggaagt caatatggca ccaatattac
    13561 tattaatgat ggtgacagaa ttacaggaga taccgccgat ccatcaggaa acctctatgg
    13621 tgtaatgacc ccagcaggaa acacgcctgg caatatcaac ctgggtaatg atgtcaccgt
    13681 caatgtcaac gacgcctctg gatatgcaaa aggaatcatt attcagggca aaaacagctc
    13741 cctgacagct aaccgactca cagtagatgt tgttggtcaa acctctgcca tcggca
//
LOCUS       DAJTMK010000107        13642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTMK010000107 DAJTMK010000000
VERSION     DAJTMK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13642
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.108"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..1868)
                     /gene="speC"
                     /locus_tag="OO135_002707"
                     /pseudo
     CDS             complement(1..1868)
                     /gene="speC"
                     /locus_tag="OO135_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2266..2973
                     /locus_tag="OO135_002708"
     CDS             2266..2973
                     /locus_tag="OO135_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5398505.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3079..3154
                     /locus_tag="OO135_002709"
     tRNA            3079..3154
                     /locus_tag="OO135_002709"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3112..3114,aa:Phe,seq:gaa)
     gene            complement(3303..3839)
                     /gene="yghD"
                     /locus_tag="OO135_002710"
     CDS             complement(3303..3839)
                     /gene="yghD"
                     /locus_tag="OO135_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR5398506.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3841..5019)
                     /gene="gspL"
                     /locus_tag="OO135_002711"
     CDS             complement(3841..5019)
                     /gene="gspL"
                     /locus_tag="OO135_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR5398507.1"
                     /translation="MSSILEIFFPLCATDPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5016..5993)
                     /gene="gspK"
                     /locus_tag="OO135_002712"
     CDS             complement(5016..5993)
                     /gene="gspK"
                     /locus_tag="OO135_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR5398508.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWVFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5996..6595)
                     /gene="gspJ"
                     /locus_tag="OO135_002713"
     CDS             complement(5996..6595)
                     /gene="gspJ"
                     /locus_tag="OO135_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR5398509.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6592..6963)
                     /gene="gspI"
                     /locus_tag="OO135_002714"
     CDS             complement(6592..6963)
                     /gene="gspI"
                     /locus_tag="OO135_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR5398510.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(6960..7523)
                     /gene="gspH"
                     /locus_tag="OO135_002715"
     CDS             complement(6960..7523)
                     /gene="gspH"
                     /locus_tag="OO135_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR5398511.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7527..7982)
                     /gene="gspG"
                     /locus_tag="OO135_002716"
     CDS             complement(7527..7982)
                     /gene="gspG"
                     /locus_tag="OO135_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR5398512.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7999..9222)
                     /gene="gspF"
                     /locus_tag="OO135_002717"
     CDS             complement(7999..9222)
                     /gene="gspF"
                     /locus_tag="OO135_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR5398513.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9222..10714)
                     /gene="gspE"
                     /locus_tag="OO135_002718"
                     /pseudo
     CDS             complement(9222..10714)
                     /gene="gspE"
                     /locus_tag="OO135_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10714..12774)
                     /gene="gspD"
                     /locus_tag="OO135_002719"
                     /pseudo
     CDS             complement(10714..12774)
                     /gene="gspD"
                     /locus_tag="OO135_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12804..13634)
                     /gene="gspC"
                     /locus_tag="OO135_002720"
     CDS             complement(12804..13634)
                     /gene="gspC"
                     /locus_tag="OO135_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCR5398514.1"
                     /translation="MFWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKT
                     FDKNDVQLISQQNWFGKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEG
                     GKQQVYLQGETLGSHNAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSD
                     EAKQAVAEPAVSAPVEIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRS
                     LFDASGFKEGDIAIALNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR
                     "
BASE COUNT         3055 a         3935 c         3523 g         3129 t
ORIGIN      
        1 cagcgctatg cgcatcctgg gggttcatca ccactgacgg gaaactctgc tggcggaaca
       61 tcgctttttg taggtctttg acgtcgaaac tgacatacag atcgtgcatc tcctgacaca
      121 actggcgcaa agtgtagtcg cgatagcgca ccggatattt gttataaacg ctcggcaaca
      181 cctcagccag cggcgagtca tcctcaatat gctgttcaaa ctgcgccaac atctctacca
      241 gttgtgccag cttctcgtgg ctttccgccg gagttaataa gaacagaatg gagttgagat
      301 cgcacttctc cggcacaatg ccgttctcac gcagatagtg cgccagaatc gtcgccggaa
      361 cgccaaagtc gctatattcg ccggtttcgg cattgatacc tggtgtagtg agtaacagct
      421 tgcacggatc aacaaaatac tgatccgcgg catatccttc aaagccgtgc cacttcgccc
      481 ccggctcaaa actgaaaaaa cggcggtcgc tggctaacac tgatgtcgga taatcctgcc
      541 acaatttgcc atcaacaacg ggcgggataa acgggcggaa cagcttacag cgcgcaagaa
      601 tagccttgcg cgactcaata cccaactcaa cgcactcagc ccacagccga cgcccactct
      661 ccccttcatg aattttggcg ttaacatcca gtgcagcaaa cagcggatag aaagggctgg
      721 tagaagcatg gagcataaag gcgttattca accgcttatg cgggcaaaaa cgcgcctgtc
      781 cgcggatatg gttatctttt ttatggatct gcgacgtctg tgagaatccc gcctgctgtt
      841 tgtgcaccga ctgagtcaca aagatccccg gatcgttttc gttaagttct aatagcagcg
      901 gcgagctatc cgccatcatt gggataaact gttcgtaacc gacccacgcg gaatcaaaca
      961 gaatgtaatc acacagatgc ccaacggtat cgatcacctg acgggcgtta tagacagttc
     1021 cgtcataggt tcccagctga ataatcgcca ggcgaaacgg gcgcggcagc tcggcttttt
     1081 ctggcgcaac gtcgcgaatt tgctggcgca gatactcttc attaaaacag tgcgcatcaa
     1141 taccgccaat gaaaccaaac gggttgcgtg aagcttccag atagaccggc gtcgcccccg
     1201 cctgaatcag cgcgccgtga tgattcgact tatggttgtt acggtcgaag agcaccagat
     1261 cgccacgcgt taacagcgca ttcgtcacca ctttattcgc tgccgatgtg ccgttcagca
     1321 caaaataggt tttatcggca tgaaagactt tggctgcgaa tttctgcgca tctttcgcca
     1381 ataagcagat cgcccaattt tacgtcagcg ttacacatat cggcgcgaaa gacgttctca
     1441 ccaaagaaat cgtaaaaatg gcgtccggca ggatgctttt taaaaaacgc accatgttga
     1501 tgtccagggc aagcaaaggt gctgttgccc atctcaacgt actgcgtcag cgtgtcataa
     1561 aacggtggca gcaaattctc ttcatactga caggctgcgg attccagctc cagccactgc
     1621 tgctcgttgc cgttgattac cgccgtaacg cccgcaggta attcaacagc atgttcggaa
     1681 tacaaaaaca ccggtagatg aaaaccggtg cgcttaagca acgcaagaat gccactgcga
     1741 ctatccgcag cggtaatgac gactgccgcg acgtccgtaa aatcagtatc tcccaacgcc
     1801 accacgcgac gatgagaaga aagtcgggat accagttcac tactggcggc aatattcatt
     1861 gatttcataa gcgcaaaccc gtttcgggga agtaagaata ccggacaagg tggaaaaccc
     1921 tgccccatga gatatggggc aaactggtca ccagctccga ccgccagaca tcagtaaaag
     1981 cagaaacgct ctgattttac tgttgtcctg cagtgagcgt gcgttaactt caccgcatga
     2041 gcagtaacat agaaagggaa acgtttcgcg cgaaacggcg ataagcgaga gaatgtaagg
     2101 aggtggcgtg catcgggcaa actccgtaca gagaggagaa aattcgcgca atcatggcac
     2161 ctttcgctaa agcgtgcaag ccaggacttt gcgaccagac aagccatcaa caaggcaaaa
     2221 agttcataat aagaaaatca aacaatacag ttaacaggaa ctcttgtggt catcggccct
     2281 tttatcaacg ctagtgcagt cttactgggt ggcgttctcg gcgcactgct cagccaacgc
     2341 ttaccggaac gtatccgcat ctccatgaca tctatttttg gtctggcatc gctggggatt
     2401 ggtattttac tggttgtgaa atgcgccaac cttccagcga tggttttagc tacgctactt
     2461 ggcgctctaa ttggcgaaat ttgtttgctg gagaaaggtg tcaatacagc agtcgccaaa
     2521 gcacaaaatc tgtttcgtca ctcacgtaag aagccaacgc atgaatcttt tattcagaat
     2581 tatgtcgcga ttattgtcct gttttgcgcc agcggcaccg ggatcttcgg ggcgatgaac
     2641 gaagggatga ccggcgatcc gagtatttta atcgccaagt catttcttga tttctttacg
     2701 gcgatgatct tcgcctgctc gctgggtatt gcggtgtcgg taattagtat cccattactg
     2761 atcatccagt taacgctggc gtgggctgcc gcgctgatat taccgctgac cacaccgtcg
     2821 atgatggcag acttcagcgc cgtaggcggt ttattgctgc tggcaaccgg attacgcatc
     2881 tgtggcatta aaatgttccc ggtggtcaac atgcttccag cactccttct ggcaatgccg
     2941 ctttccgccg cctggaccgc ttggtttgcc tgacaatgcg tgcaatagcg gcaaagtgat
     3001 gatagattgt gcagtctgca gtaaattgaa gaaatttgat tgacgagacg aggcgaatca
     3061 ggtttaatgc gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc
     3121 cgtgtccttg gttcgattcc gagtccgggc accactaatt cttaagaacc cgcccacaag
     3181 gcgggttttt gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc
     3241 tgggggaatt actctcgcca cgttaacgag agtaatttta ttgatactaa tctcctgata
     3301 ctttaccccc gtccaaactc cagccgctgc acattcacca tcccagtctt ctcagcagca
     3361 ctggcatcaa tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc
     3421 agcaggtcat taaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct
     3481 atccgcttga tgactaccga gtgcgcggaa gcactgtcac tgataacccg cgatacctgc
     3541 gcaggcgttg ttgtggcgga ttttcgcgcc gcaataatat ccggcgcggc gctcctcagt
     3601 ctggtgttca tcgccaccag ttgctgcaac attgtctcct gttgtactat ccgttcgctc
     3661 aacggctgcc agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt
     3721 aacatgccct tttcacgcgg agaacgcccc gctaggtgtt gtgccagcca gtgttcgcca
     3781 cggcttaact ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg
     3841 ttatttcctc cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaacgcgtc
     3901 ctgttgcaca acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt
     3961 cgcggcccgt agctggaggt gaagtatctg gcgtttttga tcaaaggtaa aaccacgcat
     4021 ttcgatgtcg gaaagtgacg cagatttcag ggtgctggct atcgctgaca actcggcgag
     4081 cagccgggta tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg
     4141 taaattcaca atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt
     4201 gcggctttgc gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag
     4261 cgcaactaga atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg
     4321 gtagctgacg cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt
     4381 ggtaacggcg ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga
     4441 tagcttttcc ggtgcaatgc cgactacggt tagcgaaagc ggtaaatcct gctcattgag
     4501 ctgggcgcgg aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc
     4561 aatgcggcag ataacccgtt gcgcatcgcc agccataacc ccacaaggaa tggacatcca
     4621 gtctggcgcg acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg
     4681 catatgctgt tggtgaatca ctgctacggt tgccagttgc tggtcgatat tcaacggggc
     4741 gaaatgcagt tcatcgatat cctggttcag ctcttcttcc agtaaggcgg gcagaatggt
     4801 cggtatctgc ttacgggcga catcaggcag ttcaacctgc cagacgctga tccattcgcc
     4861 gggaatgtag agtcgaatcg catcggtttg cagccattgc tggagctgtt cgttagcaac
     4921 gtcagaccag ataccgtgct ccacatcggg tgtacggcgc tgccaatgga tgggatcagt
     4981 ggcgcaaagc gggaaaaaaa tctcaaggat ggaactcact cactttctcc tgtctgatgc
     5041 cagagaaccg aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca
     5101 atctcattca cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg
     5161 gtttttagct gttttttagt acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac
     5221 tgatcgacat cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaacg
     5281 gggcttaacc acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta
     5341 ttgatgttga tttgctggcg gatcatcggc agcgcacaga ccagcggttt cagtttttga
     5401 taaagcccgg cgtccattcc ctgtaccacg cgcatctcac tgatatcagc cagcggttga
     5461 ttggcggcgt agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccagacgt
     5521 gtctgcacgc tgcggtcctc gtcaataaac acccacaggc tttcggctat cagttcggcc
     5581 cgataagcag gcacatctag gcgcgagatc agggcaatca actgttgtac cgcaagcgga
     5641 cgcgacgccg ttgtcggctg agcaagggca ttcaggttaa agcaagcctg tgcgtcacgc
     5701 agggtgaccg caatttgccc ctgcggcaga gggaagaaac gagggccgga tgcccacggc
     5761 tgtgccagat gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg
     5821 ctttccgcac tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc
     5881 ccaagttgtt gctgcatccg cccggaaagc gtgatggtta ccagcatcat aaccgccagc
     5941 aataccagca ccacgaccag tgccattccg cgttttggtg gtgaggtgat catgattatt
     6001 gcggcccgcg taacaaccag atgcgttcaa tctcgcccca ttgcggcgaa tgcagggtca
     6061 tgcgtaccgc cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtgccgt
     6121 cgtagaactg caaacggagc gaatcggccg gaatcaactt ttgcattgtc ggcttcacgc
     6181 tgcctgcggc atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt
     6241 aaccgacggt gagcagattg ctgcgcggca gacgcatcaa cgggttaacc acaccgccgc
     6301 gcacaaaacg cattccttca ctctcagacg ccagcacgcc agcgccagcc agtaaagctg
     6361 gttcacgctg gccctgatcg cctcttaccg gacgcggcat catttgggtc agatcgtggg
     6421 tcagaaaact catcgtttgc tgcatgaggt ttagtttttg atcgtgatcc gcgacggcgc
     6481 tattcacgcg cgtgacgccg ttagtcacct gctgcgccat cagtgccagc gaggcaaaaa
     6541 tggcaattgc caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg
     6601 gcctcccacg gcgctaaacc aggcgcgtcg tgactgaatc accgacgaaa agtcttcgtg
     6661 acggctgact tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt
     6721 tcgccagtac cagcggcttc ctgccatatc gctctcgccc tgatgcctga ccgcccgctg
     6781 ctcacgggtc atcaggtaga gcagcgcagt ctggttttca gctacccagc ccgctaccgt
     6841 tttttcctca agaacgtgct gattactcag cgcaccgctg gcaatctgta acaccgccgt
     6901 ggcagccagc gcaaaaatcg ccagcacgag catcacttcc agcaaggtaa atccgcgctt
     6961 catggcatcc tctcatcaca ttgattgagg gataacgcgc catcgtgtgc cagctttacc
     7021 gcccaacatg cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca
     7081 aaaggcgaaa aacggatctg cggcgtcttc tttttcgcct ctttttgcaa ctccagttca
     7141 atatcgtgca gcgtcaggcg acgacgttgc agctccagcg catattcctt ctgccagata
     7201 tcacttccgg gttgtaacag catctgcacc tgtttcggca ccgtaacctg tgccgataac
     7261 cgggtcgcag aaacgggcaa ccactgcccc tgacgacgct gcataaactg atagccaggc
     7321 gggtcgatta gcacaccgag tgtctgtcct tcaatcactg ccctgtcctt aaactgcgca
     7381 aagcgagtca gaaaatcctg tgccgctttt ttcgcgggcg gttcagaatc ggtcgcaaac
     7441 gtctgtacca cgcccgcact ggcaaggccg ataaggaaaa tcaccagcat gatttccaga
     7501 agtgtgaatc cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata
     7561 tctgcgccag cgccttcccc gttttcctga ccatccgccc ccagggtata aacatcaaac
     7621 agtccttttt cacccgggct gagatactga taatcattgc cccacggatc ctttggcagt
     7681 cgtttgatgt atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg
     7741 atcagcgctt ccagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata
     7801 tctagtgcgt tttccagcgc cacgatatcg ctgatggctt tttgccgatc ggctttctct
     7861 ttgttgccca acaggttagg caccaccaga cttgccagga cgccaagaat aacaatcacc
     7921 accatcactt ccagcagggt aaaacctgcc cgtggtttgt gtgtgcggga taacgaattc
     7981 atgtgataac tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag
     8041 gatggcgatg acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa
     8101 cagccccaac gccagcccca cctgggtatc aaattcccgt tcctggttaa cagcggcctg
     8161 ctcaagcatg gtttccagtt cgccgctctg ttcgccggaa gcgatcatgt acagcatcat
     8221 cggcgggaac aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg
     8281 atctgccgcc agcagcagtt gttgttcgac atagcgattt gccgacacgg cggcagcggt
     8341 ctgaatgcct tccagtagcg gaacactgct ggcggtgagg atgcttaatg tgcgggaaaa
     8401 acgcgccgta ttcagcccgc gcgcaacacg gcccgtcacg ggcagacgca gcaaggtttt
     8461 atcccagcgc aggcgcatag taggattttt aagtagccat tgccccagca ccagaagcgc
     8521 cagcaatccc gccagccagt aaacgccgct ggcctgtaac gcatcactca tggcgataag
     8581 tgcacgggtg gaggagggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg
     8641 caccactgcc gtcagcaaaa tagtgaccac gcccgttgcc accaccagca taaccagcgg
     8701 atagagcatg gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc
     8761 caggcgattg agcaccacgt cgagatgccc ggatttttct ccggcagcga ccatcgaaca
     8821 aaacagggaa tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tataaccttc
     8881 ctgaatccgg ctgcgcagcg ccattccgag gctcttcaca tgcagttttt cactttgctc
     8941 actgaccgcc tgcaagcagg tttccaacgg cattgctgcc tgcaccagcg ttgccagttg
     9001 gcgcgtgaac agcgccagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg
     9061 caacattccc cctgccgatg cattcatcct ggcttcaatg tgcacgggga taagctcttt
     9121 accgcgcaac aactggcggg catgacgcgc ggaatccgcc tcaatcatgc ctttggtttt
     9181 gcgaccatta cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc
     9241 cgcagaactt catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg
     9301 atacccgcag agtgttgccg gacataacgt tccagctcca gctccccggc ctggcggtgg
     9361 atcaaatcac gcaatgtggc atccaccacg tcagctcatg gatggcagtc cgtccgcgga
     9421 aacctttgtg attacaggcg gggcagccct gcggatggta cagagtgacg gttcgcgcat
     9481 cggtaatgcc cagcaggcgt ttttcttcgt cagtggcagg cgcggcctga cggcagtcgg
     9541 gacacagcgt gcggaccagt cgctgcgcca tcacgcccgt cagactggaa gagagcagga
     9601 aaggctccac gcccatatcc tgcaaacgcg tgatcgcccc caccgccgtg ttggtatgca
     9661 gcgtggaaag gaccaggtgt ccggtaagcg atgcctgaac agcgatttct gcggtttcgg
     9721 tatcgcggat ttcaccgacc atcaccacat ccgggtcctg acgcaaaatc gcgcgcaggc
     9781 cacgggcgaa ggtcatgccg acgcgggtgt taacctgtgt ctgaccgatc ccttcaatca
     9841 tgtattcgat ggggtcttca accgtgagaa tgttacgcga atggttgttc agctcctgca
     9901 atccggcgta cagcgtggtg cttttgccgg aacccgtcgg ccccgtcacc agaaagatgc
     9961 cgtgcggttt gtgtaacagc tggcgcaact gcacggtcag ttgctggcta agccccagac
    10021 gctccagcgt caggcgggcc tggtttttgt ccagcagtcg cagcaccacg cgctcgcccc
    10081 aggcagaagg catggtggag acacgcacgt caatcgcccg gccgcccagc agcagcgcaa
    10141 tacgtccatc ctgcggtacg cgcttttcgg cgatatccag ccgtgccatc accttgatac
    10201 gcgataccag cagcgaggcc agtttgtgcc cagggcgcag catttcatgt aatgtgccgt
    10261 caacgcgaaa acggatcacc agactctttt caaacgtctc gatgtggata tccgaagcgc
    10321 cttctttaat cgcctctgcc agcatggcgt tgatcagttt gatgatcggc gcatcgtcgt
    10381 cactttccag cagatcttcc gtttctggca gttcttccgc gagagtaaaa aagtcctcag
    10441 cagaaccgag atcttccatc agctggcgag cctcggaaga gtcccgctgc cagaccgcat
    10501 tcagccgctg ttcaaattcg gcctcgtcga tttgccgcag cgtaaagggc gcgttcagcc
    10561 cccgctgcag ctcctgcaaa acagagagcg atagcggatg aacgtggagg atctccagcg
    10621 acgcttcgca ccatgccacc aggctaaacc gacggctgaa actgtagggc agacgcacgg
    10681 tgttggcggt ggtttcctgt gctacaggca ccattaacgc gttctcccgg cattgaggaa
    10741 cgcgcgaact tccggcggta aggcctggtt ttgcgctggc agtaccggct gtgcggtgtg
    10801 cggcatcagg cttaaacctt gctcatcacg atagatctgt tcggcgcgca tatagttata
    10861 tttgcgctgc gacacgccgt ctgccgccat accgtcacgc agaatggtcg ggcggataaa
    10921 caccatcagg ttacgttttt cttttttatc cgccgtcgat ttaaacagtt taccaatcaa
    10981 tgggatatcg cccagcagag gcactttcgc cacgctttct cccgcctgat cgtccatcag
    11041 accaccaagc acaatcagct caccatcgtt agccagcacg gtggttttca gtttgcgctc
    11101 accaaacacc acgtcgaggc tggtctgtcc ttccaccttc gagacttcct gctcaatcac
    11161 catctgtacc gcgttacctt cgttaatctg cggcgtgact ttcagcatga tgccgacttt
    11221 tttcctttct actgtattga aaggattgct gttattggag ccaacggtag agccggttaa
    11281 taccggaacg tcctagccca ccatgaagaa ggcttcctgg ttgtccagcg tagtgatgct
    11341 cggcgtggag agaacgttcg agctggagtc gtttttaacc gcctgcacca gtgccatcca
    11401 gtcgcctttc accacgccaa ccgccgtacc gctaaatcca gaaagaagct gggcgagtgt
    11461 ggagagatcg ccgttggtat ccggattaat ggtggtagcg ccgttttcac tgatcaccgt
    11521 cgagcctttc tgcggtttcg cctgagaaat ggctgccccc agcgtaccga tagggatctg
    11581 cgtaccatta gcaaactgca ttaatccggc atctttcgac gcccactgca cgccgaagtt
    11641 gatattgcta ccttcggcaa cttccacgat caacgcctcg acgtgcacct gagcacggcg
    11701 aatatccagt tgttcaatta cgctttgcag cgactgcatg atgtcctgcg gcgcggttac
    11761 aatcagggca ttactgtgtt tgctggcggc gatggataca acctcacgcc cgctaccgac
    11821 cgtgccttcc gcctcttctt tagccgccgt gagcgtgccg ctgacctgtt tcagcacatc
    11881 gaccagatct tcggctttgc tgtatttgag atagaaaacc tggctgttgc cgctgcgttc
    11941 catttctgag tccagccgac ggatcaggcg gcgcattttg tcccgcgtgg ccgggtcacc
    12001 actgacaatc acactgttgg tgcgttcgtc ggcgacaatt tgagatttca gcgtcgcagg
    12061 ctggttctcg ccgctgtttt tcgtcaggct ttccagcacg cgggcgattt ccgaagccga
    12121 ggcgttatcc agcgggatca cctcttcggt gcgattaccc gcgtgatcca cgcgctggat
    12181 cacttccgtc aggcgctcca caacggaggc gcgtccggtg agcataatca cgttggaggg
    12241 atcgtaatta acaacgttgc ctgagcctgc gctgtcgatc atctggcgca gaatcggtgc
    12301 cagctcgcgt accgacacat tacgtaccgg cactactttg gtgaccattt catcgcccgc
    12361 gtagttgtcg ctgccttcac cgaccagcgg cagcggctcg actttcgcag cgctggattt
    12421 caccaccttc agcacgtcgt tttccatcgg tacgacggca tacccctgtg cttccagcag
    12481 gttaaggaat agctggtaat actggcgttc attgagcggg gtcatggtgc gaatactcac
    12541 tttcccctgt acgcccggcc ccataatgat ggttttatta aggttagcgc cgacggtttc
    12601 gatgaacgat ttcaggtcgg tatctttaaa attagcggtg aaagtggctt cttccgccca
    12661 gaccggtgaa ctgcataatg ccgctgccag caccagcggc agtaaacgct tttttgtttt
    12721 gaggccagtt gtcttcttac gccagaccga caacgtaata tcacgccaaa acacgatgaa
    12781 tgattctcct ggattaaatg cggttagcgc agcgcgatgg aaatgtcgtg gcgcgcaccc
    12841 ttgcgtaaaa ccgtaagttg aatggaatcc attgaaggta actgccgcat cagagcaatc
    12901 attgctcgtg gatcagtgaa atcctgctga tttagcgcaa tggcgatatc gccttccttg
    12961 aaaccgctgg catcgaacag agaacgatct gcccccggct tcactgcata accgacaatt
    13021 ccctccttac gcacaggcgt aagctggata tagttaaaaa ttttctgcgg atctttcgcc
    13081 agtgcctgac gcacggcagt cggtatctca actggcgcac tgacagcagg ttcagcaaca
    13141 gcttgctttg cttcgtcact gacagctttt ttgttggtcg cggcaacggt ggaacgctcc
    13201 tcttcagcca ggctcaggcg ctctattttt ccctgatagc gcagcatcac atggtcgcga
    13261 ttgatttcct caatcaccgc gttgtgcgag ccaagcgttt caccctgcaa atagacctgc
    13321 tgtttaccgc cttcttcaat aaccgcgccg ggtctggcac caaaggcgat cccgcgcagc
    13381 accacattaa gacgcgtttc tgccacaggc acaggttcgg gttgttttac cggcgtggcg
    13441 acgggctgat atttgccaaa ccagttttgc tggctgatta attgcacgtc atttttatcg
    13501 aacgtttttg catccgcacg gggcggttta ttcgccgatg gggaaacagc cgtatattcc
    13561 gcagaaaagg agaaatagcg ccagagtgaa tgcgccactt ttgcagaaat aataagcagc
    13621 atcagccaga acatcccgcg ta
//
LOCUS       DAJTMK010000108        13484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTMK010000108 DAJTMK010000000
VERSION     DAJTMK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13484
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.109"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(103..3324)
                     /gene="carB"
                     /locus_tag="OO135_002721"
     CDS             complement(103..3324)
                     /gene="carB"
                     /locus_tag="OO135_002721"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5398515.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3342..4490)
                     /gene="carA"
                     /locus_tag="OO135_002722"
     CDS             complement(3342..4490)
                     /gene="carA"
                     /locus_tag="OO135_002722"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5398516.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSALGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KHHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4946..5767)
                     /gene="dapB"
                     /locus_tag="OO135_002723"
     CDS             complement(4946..5767)
                     /gene="dapB"
                     /locus_tag="OO135_002723"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5398517.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5934..6848)
                     /gene="rihC"
                     /locus_tag="OO135_002724"
     CDS             complement(5934..6848)
                     /gene="rihC"
                     /locus_tag="OO135_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5398518.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6914..7864)
                     /gene="ispH"
                     /locus_tag="OO135_002725"
                     /gene_synonym="lytB"
     CDS             complement(6914..7864)
                     /gene="ispH"
                     /locus_tag="OO135_002725"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5398519.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7866..8315)
                     /gene="fkpB"
                     /locus_tag="OO135_002726"
     CDS             complement(7866..8315)
                     /gene="fkpB"
                     /locus_tag="OO135_002726"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5398520.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8384..8878)
                     /gene="lspA"
                     /locus_tag="OO135_002727"
     CDS             complement(8384..8878)
                     /gene="lspA"
                     /locus_tag="OO135_002727"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5398521.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8878..11694)
                     /gene="ileS"
                     /locus_tag="OO135_002728"
     CDS             complement(8878..11694)
                     /gene="ileS"
                     /locus_tag="OO135_002728"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5398522.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11737..12678)
                     /gene="ribF"
                     /locus_tag="OO135_002729"
     CDS             complement(11737..12678)
                     /gene="ribF"
                     /locus_tag="OO135_002729"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5398523.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12686..12904)
                     /locus_tag="OO135_002730"
     CDS             complement(12686..12904)
                     /locus_tag="OO135_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5398524.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13007..13270
                     /gene="rpsT"
                     /locus_tag="OO135_002731"
     CDS             13007..13270
                     /gene="rpsT"
                     /locus_tag="OO135_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5398525.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3147 a         3807 c         3400 g         3130 t
ORIGIN      
        1 caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt attacttatt
       61 cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga tctgcgcgtg
      121 catttcctgc accgaaatta ctttttcggt cgcgtcggca ttcagcgcca tcgcggtagc
      181 gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac tgcgacggat
      241 cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg ttgatgatgt aggtatattc
      301 gccattcttg atgcggtcct gaatgtgcgg acggccttca tgcaccttgt tcaccagacg
      361 cggattaatg cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa
      421 gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac
      481 ggaaagcaac gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc
      541 aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc ccggtggagc gcatttctgg
      601 ccctaacagc gggtcaacac ccgggaattt attgaacggc agcaccactt ctttcaccga
      661 gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt tgccagccat
      721 cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga acggaacggt
      781 acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca ccgcaaactg
      841 cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct gctggcgcat
      901 cacatcctga atttcctgac ttaaggtgta ggctggcaga gaacatgcgg agtcaccgga
      961 gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca tttcgccgtc
     1021 gcagatggca tccacgtcaa cttctaccgc gtcatcaagg aagtgatcca gcaacactgg
     1081 cgcatcgtta gacacgctga ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata
     1141 gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgtaccacca gcgggtagcc
     1201 aatctctttc gccttctcta ccgccatttc aatagcggta acggtggcgt tcgccggttg
     1261 tttcagtttc agacgctcaa ccgcatgctg gaagcgttca cggtcttctg cacggtcgat
     1321 agcatccggg ctggtgccga taaccggtac gccagcagct tccagcgcgc gcgccagttt
     1381 cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa tacgcacgat
     1441 ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc
     1501 ggtggagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt cttcgcgcag
     1561 cgccagcgag gcgtgtacac agcagtagtc gaattcgata ccctgaccga tacggttcgg
     1621 gccgccgccg agaaccatga ttttttcacg gtcggtagac ggattcgctt cgcactcttc
     1681 ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac aggtatccac
     1741 gcgcttataa accgggtgca ggtcatactg gtcgcgcagc ttgcggattt ccgcttcgcg
     1801 cacgcccgcc agttttgcca gacgcgcatc ggcaaaacct ttgcgtttca gctggcgcag
     1861 gaattcagcg ttcaggccag tgatgcccac ttccgctact ttctcttcca gacgcaccag
     1921 ttcttcaatc tgtaccagga accagcggtc aatattggtc aggttgaaca cgccgtccac
     1981 agacaggccc gcacggaagg catcggcgat gtaccagata cgctctgcgc cagcatcttt
     2041 cagttcgcga cggattttgg ttaacgcttc cgggtcatcg aggctcactt tcgggtcgaa
     2101 tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg
     2161 accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc
     2221 agcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc
     2281 gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga gggtgtaacc
     2341 caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc
     2401 cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt
     2461 caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca gcaccgccat
     2521 cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg gcgcgacagt
     2581 gatggagtca ccggtatgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat
     2641 gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga
     2701 ctcatcaatc agcagctctt tggttgggga gagatccaga ccgcgggcgc aaatttcttc
     2761 aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg
     2821 aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca tcgtgtgtgc
     2881 gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga aacgacggcg
     2941 gtcttctgct ttatcaatcg catcggcggt cgcaccaatc atagtcacgc cgaactcttc
     3001 caacacgccc tgacgctcca gctccagcgc gcagttcagc gccgtctgac cgcccatcgt
     3061 tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtactactt cccagtgaat
     3121 cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg ccgggttgga
     3181 gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt gcgcgccaga
     3241 gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact
     3301 ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg
     3361 tactgctcaa ttaactcgat aaagtggtcg aacaacggcg ctgcgtcgtg tggcccaggg
     3421 ctggcttccg ggtgaccctg gaagctgaac gccggtttat cagtgcgatg aatgccctgt
     3481 aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa tgttgcttca
     3541 tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc cacatcttta
     3601 accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc
     3661 agcgccagca gctgatgacc gaggcagatg ccgaataccg gaatatcggt ttcgaggaat
     3721 ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg
     3781 aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg
     3841 gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca
     3901 taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag gccgccggtc
     3961 aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc ttttgccaga
     4021 tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccaacgc cgcatccggg
     4081 ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc
     4141 ttacgggtat cgatatcggc aatcgccacg atgttatggt gcttcaggta agaagagagg
     4201 tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgt
     4261 gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga
     4321 taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg
     4381 gtcattgaag tattgaaaac gacttcccca agcgccgaac ctgttgcccc tatggcccga
     4441 ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacaccctcc
     4501 atagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta aatcaatgca
     4561 aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg ggcgctaaaa
     4621 gtcaacttaa tggcgggatt ttttatatta ttttgtggct catgcatgtt ttacaggcat
     4681 taagcgacaa aaagatcata taacctgttc tggaaaacgc ttgcgcagca agaagattgc
     4741 atgaaataaa agcgagcagg gaaaagtgga ccaaatggtc aaattttaca ttaaaacaac
     4801 aaaaacagaa gacaatacat gtaatttttg gcacttatat acaatttaaa aacataacac
     4861 taaaaataaa agggccaata attaaagccc tttataatca ataaattaaa tgtattattt
     4921 ttgcaccata acaaatattt tgtggttaca aactattgag atcaagcaca tctcgcatat
     4981 caaaaagacc actttcctta ccactcaacc acaaagccga tcttaccgcg ccgttagcaa
     5041 atgtcatgcg gctggacgcc ttatgggtga tctccagacg ctcgccaata tcggcaaaca
     5101 tcgcggtatg ttcgccaacg atgtcacctg cacgcacggt ggcaaaacca atggtgccag
     5161 gcacacgttc accggtgtgg ctttcacgac tgtagaccgc gcaatctttc agatctttat
     5221 caagggcgtg ggcgatcgcc tctcccattg ccagtgcggt gcctgacggc gcatcaactt
     5281 tatgtctatg atgtgcttca ataatttcga tatcggtgta gtcacccatc actttggctg
     5341 ctttctccag cagcttaagc atgacgttaa cgccaacgct aaaattggcg gcaaagacaa
     5401 tcgcaatatc ggcggcggcg tcacgaatcg cctgtttacc ggcttcgtca aaccccgtag
     5461 tgccgatcac catccctttg ccatgctggc gacaaaaagc gagatggttc agcgtacctt
     5521 ccggacgggt aaaatcgata aacacatcaa aatcatcttt taccgcatcg aggctgcttt
     5581 gcacggtaac gcctgttttc cctgcaccag ccagctcacc ggcgtcgctg cccagtaaag
     5641 aagatccttc gcgctccagc gccgcgccca gctgcacgcc ttctaatgcc agcgccgcct
     5701 gaatcaactg gcggcccata cgccccccgg ctcccgcgat ggcaacgcgg atgtttgcat
     5761 catgcatagc tattctcttt tgttaatttg catagaccgt tttcagagta accagcgcat
     5821 cgctacgccg ccagccaaaa acgctgataa ttagaaatta atgataaaca gggagggata
     5881 tcagtgaaag gcatgagtcg atgaatgact gcatgccgat aacatgtgac aggttacgac
     5941 gccagagcca gcacctcagc cacccactgc tggaagcctt tcacatccag atccaatgcc
     6001 acctttacat tggctggttt gcccaggcaa ccgtcgatat caaccaccgt cgtgcctgag
     6061 gtaaattcgc cctgagtttc cactgccaca aaacacggtt tgagagtgaa caattccggg
     6121 cgcaccagcc aggcaatagc gcaaaggtcg tgcatacgca gtccactttg catactgccg
     6181 ctacggtagt ggctaaacag ggcgtggagc atttttccgg tgcgattcaa ctccggcaaa
     6241 gtagcgagat agtcaggggt taatattgcc tgattggtga catccagacc gcacatgacg
     6301 atttcgatgc cgctacggaa gacacaggca gccgcttctg gatcggcagc gatattaaac
     6361 tcggcgtttg gtgtgcagtt gccgcgacca gcagaaccgc ccatgatcac cagacgacga
     6421 atatacggtt tacattccgg gcattgtgaa agtaacagcg caatattggt taacgggccg
     6481 atggccacca gggtaacggg ctctggtgca cgcatcaggg catcccgaat cgccagaaac
     6541 gctggtatcc cgagcggctt tcggttgtgc tcaacaaagt cgtagccagc cattcccgat
     6601 tcgccgtgca cagatgccgc atcacgcggt gcgcgtacca gcggcacagc ggctccctgg
     6661 gcgagcggaa tctccgcatt ccagaaatgc agcagttgca gggcgttgcg ggtggttttc
     6721 tcaaccgaga cattacccgc gacggtggtc atcagttgca ggtcgagttc gggtgcaaaa
     6781 atcgcggcgg caatggcgac ggcatcgtca atgccggggt cagtatcgag gaagataggt
     6841 aaacgcatgt tttctccata aaaaatgccg gtaacaagac cggcattttc gcataactta
     6901 ggctgctaat gacttaatcg acttcacgaa tatcgacacg cagctctttc ggcacttcga
     6961 aaacaatgtt ttcttcacgg ccttccagcg gaatggcttc accaccgccc agctgctgca
     7021 aacgtgccac cacattctgc accagaatat ccggagccga tgcgcccgca gtcacgccga
     7081 cgcatttaac ctctttcacc cactcttcct ggatgtcttt cgcatcgtca atcaaaaacg
     7141 cgcgtttgcc catacgctgg gccagctccg ccagacggtt ggagttggag gagtttttcg
     7201 aaccgaccac caacacaact tccgcctgtt ctgccagggc gcgtaccgct tcctgacggt
     7261 tagtcgtggc gtagcagatg tcatctttgc gcggaccgac aattttcggg aagcgtttac
     7321 gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag cgtggtctgg gtcataaagg
     7381 agagcttctc ttcgtttttg accgtcagtt tccacacatc gtccggcgat tcgaccagat
     7441 acattccccc ttccgggtta ctgtactggc ccattgtccc ttccacttcc gggtgcccgg
     7501 cgtgaccgat gagaatagat tcttcgccac ggcgactggc gcgggcgact tccatatgca
     7561 ctttggtcac cagcggacag gtcgcgtcaa ataccgtcaa atcgcgactt tttgcttcgt
     7621 tacgtaccgc ctgagaaaca ccgtgtgcgg agaaaatcag gatcgcgccg tccggtactt
     7681 cgctaatctg ctcaataaag atagccccac gctcacgcaa gctatcgacc acatagcggt
     7741 tatgtaccac ttcgtgacgg acatatatcg gtgcgccgta aatggccagc gcgttttcaa
     7801 caatgctgat agcgcggtct accccggcac aaaaaccacg cgggttggcc aacaggatct
     7861 gcatgttacg cctccaatgc cggatcgatt tccagcactt caatatcaaa atgaacggtc
     7921 tgcccggcca gcggatggtt gaaatcaacg gtaatggagt cgccgttaat ttcgcggatc
     7981 acgccaggca tctcactgcc atccattgcg gtaaaaagca tgattgcgcc aatttctggc
     8041 tcgcctgcat ccataaattc acggcgggag aagtactgaa tcaggtccgg tgacggcacg
     8101 ccaaacgccg catctggctc caacgagaag gtggttttat cgcccacttt cagccccaac
     8161 aggtgttgct ccagcccttc agaaagagaa gcatcaccca ggcggaacag cgccggttta
     8221 ccgttgttgc gggtagactc ggcggtggtg ccatcgtcga gttttagcgt gaagtgcacc
     8281 aggacggcgc tattgctctg tacagattca gacatgcagg tttgctctta tatttgagga
     8341 tttttgccgg atgcggcgta aacgccttat ccggcagggt ttattattgt tttttcgctc
     8401 tagaaggcaa aaaaccttcc agcacaatca gtgccgcacc gacacagatg gcagtatcgg
     8461 caaggttgaa ggtggcgaag tgccagtcgc cgacgtagaa gtcgatcata tcgacaacaa
     8521 agccgtgcca caggcggtcg aacaggttgc ccagcgcgcc gccaataatc agcgcgtaag
     8581 cgatattgtt tagcttctgc gtggccttcg agcgatacat catcactgcc aggatcacgc
     8641 taataccaat cgcaataccg gcaaagaacc aacgctgcca gccgccgcta tcggcaagga
     8701 aactaaacgc cgcgccatag ttacgcgcat aatgcagatt aagcgacggg aacagcggga
     8761 ccgtatcccc cagagcaaag ttctggagga tcaggtattt gctgcccaga tcgataatca
     8821 gcacgactac caccagccac agccagcgta gccctgttga acagatcgat tgactcatca
     8881 ggcaaactta cgtttttcac cgtcaccggc gacgttgctg acacagcggc cgcagatttc
     8941 tgcgtgttcc gccaccttgc cgacatcctg ggtgtagtgc cagcagcgtg ggcacttctc
     9001 accttcggct ttactcaacg cgactttcag ccctttgagt acttcgctct gctgagcatc
     9061 agcaggtgcg tcgtgatagt ctgcaacggt agcgccggag gtcaacagga caaatcgtaa
     9121 ttcatcgccc agcgcggtca gtttcgccgc cagttccggt tctgcataca aggttactgc
     9181 cgcttccagc gagccaccca ctttcttgtc ggcacgcgct tgctcaatga ctttgttcac
     9241 ttcgccacgc actttcaaca gctcgtccca gaacgcatcg ttcatcgctt cgctgtctgc
     9301 caggccaaac aggccttcgt accactcacc ggtgaagacg tatttttcac gttcgcccgg
     9361 caggtagccc cacacttcat cagcggtgaa ggagaggatt ggtgccatcc agcgcaccag
     9421 cgcttctgcg atgtgataca gcgcagtctg gcagctacga cgcgccacac tgtccgcttt
     9481 ggcggtgtac tgacggtctt tgatgatgtc gaggtagaag gaacccatct caacggagca
     9541 gaagcgcatc agacgctgta ccacttcgtg gaaatcgtat gcttcgtacg ccttgaggat
     9601 gtcttcctgt gccgctttcg cacaacctac ggcccagcga tccagtacca ccatctcttc
     9661 cggtttcacc atatcttttg ctggatcaaa accgttcagg tttgccagca ggaagcgcgc
     9721 ggtgttacgg atacgacgat agctatcggc agcacgtttc aggatctcgt cagaaacggc
     9781 catttcaccg gtgtagtcgg ttgatgccac ccacagacgc agaatatccg cgcccagttt
     9841 gttcatcaca tcctgcggcg aaacggtatt gccgatggat ttagacatct tgcggccctg
     9901 accatccacg gtaaagccgt gggtcagtac ctgacgatac ggcgctttgc ctttcatcgc
     9961 ggtggagatc attagggaag acatgaacca accacggtgc tggtcagaac cttccagata
    10021 catgtccgct gcgtgaccgg caaattccgg acgcacgtca acaacagaag agtgggtaga
    10081 tccggagtca aaccatacat ccaatgtgtc cggcactttc acgtactgat cagcttcatc
    10141 gccgaggatc tctttcgcat caagatccca ccacgcctgg atgccgtcaa cttcaacgcg
    10201 ttttgccact tcttccatta gttcgagagt acgcggatgc agctcttccg tgtctttgtg
    10261 cacgaacagt gacatcggta cgccccaggt gcgctgacgg gagatacacc agtcaggacg
    10321 gttagcaacc atcgactcga tacgcgcctg gccccagtcc gggatccact gcacgccttt
    10381 gatctctttc agtgactgcg cacgcagacc tttctgatcc atgctgacga accactgcgg
    10441 cgtcgcgcgg aagatgatcg gcgttttgtg acgccagcag cacggatagc tgtgctgcat
    10501 tttctcaacg tgcagcagcg cgcctttttc ctgcagcagc gcaacgacga tgtcgttcgc
    10561 tttgaagacg ttcacgccat ccagcgtcgg ataagtgccc ggcagataag tgccgtccgg
    10621 gccaaccggg ttagcggttt ccaggccgta tttctgaccg atcacatagt cgtccgggcc
    10681 gtggccaggc gcggtgtgaa cggcaccggt accggcgtcc agggtaacgt gatcgccgag
    10741 gattgccgga acgtcgaagc ccataaacgg atgggtaaag cgcagcaact caagctccgc
    10801 accttttacc gtgccgagaa tggtgtaatc ggtcacgccg atacgctgca ttacgctttc
    10861 aaccagatct ttcgccagaa tcacggcctg accgtcgatc tgcaccagcg catagtcgaa
    10921 atcaggtgca atagagattg cgcggttcgc aggcagagtc cacggcgtag tggtccagat
    10981 caccagcgag attgggccgt taacgttgct tacggcaaat tttgctttca gtgcatcctg
    11041 atcgaccgcc tgaaaagcaa cgtcgatgga cggagaagtt ttgtcgtaat actcaacttc
    11101 cgcttccgcc agcgcagaac ggcagtcaac gcaccagtgt accggcttcg cgcctttgtg
    11161 caggtgaccg ttgccgatga ttttacccag cgcgcggatg atgttggctt cagttttgaa
    11221 gtccatggtc aggtacgggt gcgaccagtc gcccagcacg cccagacgga taaagtcttt
    11281 gcgttgaccg tcaacctggg tcgccgcgta ttcgcggcac ttggcgcgga actcggcggc
    11341 ggtgaatttc tcacccggct taccgtattc ttgctctact ttcagctcga tcggcagacc
    11401 gtggcagtcc cagccaggca catacggcga gtcataaccg gaaagccctt tggacttcac
    11461 gataatgtct ttcagaatct tgttaaccga gtgaccaata tgaatgctgc cattcgcata
    11521 aggagggcca tcatgcagaa tgaaggtttt tttgcctttt ttagccgcac ggatgatgcc
    11581 gtacagatca tcatcagtcc aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc
    11641 acgcatcggg aaccctgttt ccggcaaatt cagggttgat ttatagtcac tcatcagatt
    11701 ctcggttccg tgtttcggtt tgattacata acaggcttaa gccggttttg ttagcccaaa
    11761 aaattcgcgg gcggttaatt catcacgcgc aatctgcgct ttcagttcgt ccagcgacgc
    11821 aaatcgctgc tcattgcgta tttttttacg cagcactact tgtatatggc gaccgtaaag
    11881 gtccattgca acatctaaca aatgcacttc cagctgctgg cgaataccgg caaccgttgg
    11941 gcgtgttccg atgtttgcca cgccgggtaa cggcttttca ccgaggccca gcacttctac
    12001 cgcataaacc cctttcaccg gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa
    12061 acctatagtg cgccctaatt catcaccgtg gactacacgc ccggagatgg caaacgggtg
    12121 ccccagtaaa ctttctgcca gagccagatt gtcatccgcc agcgcctgac gcacagcggt
    12181 gctgctaata cgcacgccac cttcgcaaaa agtttgcgta ctagtgatat cgaagccgta
    12241 ttccatgcca gctttctgta ataacaagaa atcgccttca cgaccagcgc caaagcggaa
    12301 atcatcccct acggcaagaa attttacgcg caaatgcttc accagaagat cgctgatgaa
    12361 attttgcgcg gttaacgccg cgaaacgcct gtcgaaacgc acgcacagca cgtaatcaac
    12421 gccacactct gcaaggtaac gcagtttttc ccgcagccgg gtcagtcttg ccggggcttt
    12481 atcggtagca aacagttcca gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt
    12541 gcgcttgcgc ccttcttcct gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc
    12601 gtcgaaatta ccaatagtca gcacacaccc ttcttgcggg gcctggctga gattatgtat
    12661 gccgcgtatc agcttcatgt ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta
    12721 cagcggtcaa ggttaaccgg cgattgagta ccgaaatcag aaagaagtgg cttttcattg
    12781 ccatggcgca aataacggga agaaagtgac cgcctgctgc aatttttatc gcggaaaagc
    12841 tgtattcaca ccccgcaagc tggtagaatc ctgcgccatc actacgtaac gagtgccggc
    12901 acattaacgg cgcttatttg cacaaatcca ttgacaaaag aaggctaaaa gggcatattc
    12961 ctcggccttt gaattgtcca tatagaacac atttgggagt tggaccttgg ctaatatcaa
    13021 atcagctaag aagcgcgcca ttcagtctga aaaggctcgt aagcacaacg caagccgtcg
    13081 ctctatgatg cgtactttca tcaagaaagt atacgcagct atcgaagctg gcgacaaagc
    13141 tgctgcacag aaagcattta acgaaatgca accgatcgtg gaccgtcagg ctgctaaagg
    13201 tctgatccac aaaaacaaag ctgcacgtca taaggctaac ctgactgcac agatcaacaa
    13261 actggcttaa tcgccaattt gctgaagctt tgtgaaaaag cccgcgcaag cgggtttttt
    13321 tatgcctgct gcttttatga tgcgtgagag aacacactac cggttgccag atagtcaaaa
    13381 gatgctggct tttgcagaca tgccgttaca ctttctgaac tcttcgtggc ttaagaatcg
    13441 tagaaccatc ggctgataca ttctgaacaa gaatatctac tcaa
//
LOCUS       DAJTMK010000109        13387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTMK010000109 DAJTMK010000000
VERSION     DAJTMK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.110"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            262..894
                     /gene="crp"
                     /locus_tag="OO135_002732"
     CDS             262..894
                     /gene="crp"
                     /locus_tag="OO135_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5398526.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEDQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            945..3035
                     /locus_tag="OO135_002733"
     CDS             945..3035
                     /locus_tag="OO135_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5398527.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(3102..4322)
                     /gene="argD/dapC"
                     /locus_tag="OO135_002734"
     CDS             complement(3102..4322)
                     /gene="argD/dapC"
                     /locus_tag="OO135_002734"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5398528.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(4408..4971)
                     /gene="pabA"
                     /locus_tag="OO135_002735"
     CDS             complement(4408..4971)
                     /gene="pabA"
                     /locus_tag="OO135_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5398529.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(5003..5605)
                     /locus_tag="OO135_002736"
     CDS             complement(5003..5605)
                     /locus_tag="OO135_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5398530.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(5595..5762)
                     /locus_tag="OO135_002737"
     CDS             complement(5595..5762)
                     /locus_tag="OO135_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5398531.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            complement(5867..6439)
                     /gene="ppiA"
                     /locus_tag="OO135_002738"
                     /gene_synonym="rotA"
     CDS             complement(5867..6439)
                     /gene="ppiA"
                     /locus_tag="OO135_002738"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5398532.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            6710..7891
                     /gene="tsgA"
                     /locus_tag="OO135_002739"
     CDS             6710..7891
                     /gene="tsgA"
                     /locus_tag="OO135_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5398533.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            8153..10696
                     /gene="nirB"
                     /locus_tag="OO135_002740"
     CDS             8153..10696
                     /gene="nirB"
                     /locus_tag="OO135_002740"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5398534.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            10693..11019
                     /gene="nirD"
                     /locus_tag="OO135_002741"
     CDS             10693..11019
                     /gene="nirD"
                     /locus_tag="OO135_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5398535.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            11145..11951
                     /gene="nirC"
                     /locus_tag="OO135_002742"
     CDS             11145..11951
                     /gene="nirC"
                     /locus_tag="OO135_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5398536.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            11970..13343
                     /gene="cobA"
                     /locus_tag="OO135_002743"
     CDS             11970..13343
                     /gene="cobA"
                     /locus_tag="OO135_002743"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5398537.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         3170 a         3521 c         3475 g         3221 t
ORIGIN      
        1 cgtactaacc aaatcgcgca acggaaggcg acctgggtca tgctgaagcg agacaccagg
       61 agacacaaag cgaaagctat gctaaaacag tcaggatgct acagtaatac attgatgtac
      121 tgcatgtatg caaaggacgt cacattaccg tgcagtacag ttgatagccc cttcccaggt
      181 agcgggaagc atatttcggc aatccagaga cagcggcgtt atctggctct ggagaaagct
      241 tataacagag gataaccgcg catggtgctt ggcaaaccgc aaacagaccc gactctcgaa
      301 tggttcttgt ctcattgcca cattcataag tacccatcca agagcacgct tattcaccag
      361 ggtgaaaaag cggaaacgct gtactacatc gttaaaggct ctgtggcagt gctgatcaaa
      421 gacgaagagg gtaaagaaat gatcctctcc tatctgaatc agggtgattt tattggcgaa
      481 ctgggcctgt ttgaagagga ccaggaacgt agcgcatggg tacgtgcgaa aaccgcctgt
      541 gaagtggctg aaatttcgta caaaaaattt cgccaattga ttcaggtaaa cccggacatt
      601 ctgatgcgtc tgtctgcaca gatggcgcgt cgtctgcaag tcacttcaga gaaagtgggc
      661 aacctggcgt tcctcgacgt gacgggccgc attgcacaga ctctgctgaa tctggcaaaa
      721 caaccagacg ctatgactca cccggacggt atgcaaatca aaattacccg tcaggaaatc
      781 ggtcagattg tcggctgttc tcgtgaaacc gtgggacgca ttctgaagat gcttgaagat
      841 cagaacctga tctccgcaca cggtaaaacc atcgtcgttt acggcactcg ttaatcccgt
      901 cggagtggcg cgttacctgg tagcgcgcca ttttgtttcc cccgatgtgg cgcagactga
      961 tttatcaccc cgatatcaac tatgcacttc gacaaacgct ggtgctatgt ttgcccgtgg
     1021 ccgttgggtt aatgcttggc gaattacgat tcggtctgct cttctctctc gttcctgcct
     1081 gttgcaatat tgcgggcctt gatacgcctc ataaacgttt tttcaaacgc ttaatcattg
     1141 gtgcgtcgct gtttgccacc tgtagcttac tgacacagct gctactggca aaagatgttc
     1201 ccctgccctt tttgctgacc ggattaacgc tggtacttgg cgtcactgct gagttggggc
     1261 cattgcacgc aaaattgctt cccgcatcgc tgctggcagc catttttacc ctcagtctgg
     1321 cgggatacat gccggtctgg gaaccgttgc tcatctatgc gttgggcact ctctggtacg
     1381 gattgtttaa ctggttttgg ttctggatct ggcgcgaaca accgctgcgc gagtcactaa
     1441 gtctgctgta ccgtgaactg gcagattatt gtgaagccaa atacagcctg cttacccagc
     1501 acaccgaccc tgaaaaagcg ctgccgccgc tgctggtgcg ccagcaaaaa gcggtcgatc
     1561 taattaccca gtgctatcag caaatgcata tgctttccgc gcaaaataat actgactaca
     1621 agcggatgct gcgtattttc caggaggcgc tggatttaca ggaacatatt tcggtcagtt
     1681 tgcatcagcc ggaagaggtg caaaagctgg tcgagcgtag ccatgcggaa gaagttatcc
     1741 gctggaatgc gcaaaccgtc gccgctcgcc tgcgcgtgct ggctgatgac attctttacc
     1801 atcgcctgcc aacgcgtttt acgatggaaa agcaaattgg cgcactggaa aaaatcgccc
     1861 gccagcatcc ggataatccg gttgggcaat tctgctactg gcatttcagc cgcatcgccc
     1921 gcgtgctgcg cacccaaaaa ccgctctatg cccgtgactt actggccgat aaacagcggc
     1981 gaatgccatt acttccggcg ctgaaaagtt atctgtcact aaagtctccg gcgctacgca
     2041 atgccggacg actcagtgtg atgttaagcg ttgccagcct gatgggcacc gcgctgcatc
     2101 tgccgaagtc gtactggatc ctgatgacgg tattgctggt gacacaaaat ggctatggcg
     2161 caacccgtct gaggattgtg aatcgctccg tgggaaccgt ggtcgggtta atcattgcgg
     2221 gcgtggcgct gcactttaaa attcccgaag gttacaccct gacgttgatg ctgattacca
     2281 ccctcgccag ctacctgata ttgcgcaaaa actacggctg ggcgacggtc ggttttacta
     2341 ttaccgcagt gtataccctg caactattgt ggttgaacgg cgagcaatac atccttccgc
     2401 gtcttatcga taccattatt ggttgtttaa ttgctttcgg cggtactgtc tggctgtggc
     2461 cgcagtggca gagcgggtta ttgcgtaaaa acgcccatga cgctttagaa gcctatcagg
     2521 aagcgattcg cttgattctt agcgaggatc cgcaacctac gccactggcc tggcagcgaa
     2581 tgcgggtaaa tcaggcacat aacactctgt ataactcatt gaatcaggcg atgcaggaac
     2641 cggcgtttaa cagccattat ctggcagata tgaaactgtg ggtaacgcac agccagttta
     2701 ttgttgagca tattaatgcc atgaccacgc tggcgcggga acaccgggca ttgccacctg
     2761 aactggcaca agagtattta cagtcttgtg aaatcgccat tcagcgttgt cagcagcgac
     2821 tggagtatga cgaaccgggt agttctggcg atgccaatat catggatgcg ccggagatgc
     2881 agccgcacga aggcgcggca ggtacgctgg agcagcattt acagcgggtt attggtcatc
     2941 tgaacaccat gcacaccatt tcgtcgatgg catggcgtca gcgaccgcat cacgggattt
     3001 ggctgagtcg caagttgcgg gattcgaagg cgtaatacag gctaaaccat cggataaggc
     3061 attcacgccg catccgacat attttgcctg atgcgtctta tttacgcccc aaccaccttc
     3121 gccaccgcgt gggcgaaacg ttgcatccct tcatcgatat ccgcatcttc caccaccagc
     3181 gacggtgcta aacgcatcac atccggtccg gcattcagca ccattacgcc aacctctgca
     3241 cccgcataca ggaaatcacg cgcccgacct ttgtagtgtg gtttcagctc cgcgccaatt
     3301 aacagcccca taccgcgaat atcgctaaac acatcgtact gctgatcgat cttctgcaaa
     3361 tggtcaacaa aacgctggcg tttcgcctga atgccttcca gcacttcagg ggtattgatg
     3421 atatcaaacg ccgcgcccgc tactgcacag gccagaggat taccgccgta ggtggaaccg
     3481 tgagaaccag gatgaaacgc agaagcaatt tccgccgtgg tcagcatggc gctaatcggg
     3541 aagccgccgc ctaacgcttt cgcagaagtc agaatatccg gcgtcacacc gtagtacatg
     3601 taagcaaaca aatcgccagt ccgccccatc ccgcactgca cttcatcaaa caccaataat
     3661 gcctgatgtt gatcgcacag ctcgcgcaag ccctgcaaaa actctggcgt cgatgccgtc
     3721 acaccgccct cgccctggat cggctcaacc accaccgcac aggtgtgatc atccatcacc
     3781 gctttcactg catggagatc gttaaaggga acgtggatga tgtctgccgg tttcggccca
     3841 aagccgtcgg aatattttgg ctgcccaccc accgaaacgg taaacagcga gcgaccatga
     3901 aaagcgttat ggaaggcaat aattttggtt ttgaacgggc tatgacgcac acaggcgtaa
     3961 tggcgtgcca gtttaaaggc ggtttcgtta gcttccgtgc cggagttcat aaacaccacg
     4021 cgttcggcaa acgtcgcctc aatcagtttg cgcccaagac gcagcgccgg ttcattggtg
     4081 aaaacgttac tgatatgcca cagagtttcg ccctgggttt ttaacgcgtt caccagcgca
     4141 ggatggcaat ggcccaacgc cgtaactgca atgccacccg cgaaatcgac atactccttg
     4201 ccttgctgat cccagattcg gctgccctga ccttttaccg gaataaactc tgccggagca
     4261 taaatcggca ggatcacttc atcgaaagtc gcgcgtgtaa ttgctgtttg ttcaattgcc
     4321 atctcatgat caccctgtta cgcataaata aatgtgaaat tacaatcaca aaatatgcat
     4381 aaaaaatcac tgaatggcaa tcggaaatca gagatgcagg aaattagcca gcagttgatg
     4441 tccttgttcg ctaagaatac tttctggatg gaactgcaca ccttccagat cccactggcg
     4501 atggcgaatc cccataatct cgcgggtttc gctccaggcc gtcacgtcaa agcacgctgg
     4561 taatgagcca ggttccacca ccagcgaatg gtagcgtgtc acggtaagtg gatttgccag
     4621 cccccgaaat acgccctcac cgttatgtgt aatcggcgag gttttgccgt gcatgacctt
     4681 tgcggcgcgc acaactttac cgccaaatgc ctgcgccatt gcctgatgac cgaggcagac
     4741 gccaagaatc ggcaagcgcc cggcatagtg gcgaataacg tcaagggaga tcccggcttc
     4801 atctggcgta caggggccgg gtgagatgac aattttttgt ggtttaaggg cgtcgatatc
     4861 cgccaacgtc aacgcatcgt tgcgcttaac cagcacatcc gcccccagtt cacaaaagta
     4921 ctggtagagg ttccaggtaa aagaatcgta gttatcaata agcaggatca tggcggctcc
     4981 ggtacgaaag aaccgcgcta ttttactcag attccccggc ttcgcttacc actttgctaa
     5041 atatcatctg cagtgcggtg agatccccca ttgccccact ctgatttgcc tgattccagg
     5101 cctctttttc gataccctgc cagctcagtt gatatccggc atgaatcgcc agttgctcga
     5161 agaagatccg ctgtgccaga ccacttccca cccggaaggg atgcagcacg ttgatttcac
     5221 aatagtaatg cgccagccgc tcgacgaact tcgttttctc caggccaacc agataacctt
     5281 cttcctccag atcctgcatc agggcattgc cctctttttc tatataagca aagtggcaga
     5341 acggcgtatc gccctgataa atatcaactt cacgcagttg ccctgcccag tcgaaaatat
     5401 cctgatacag ctggcgatgg atagttcgca aatgcggtaa accgcgcacc agcggaccaa
     5461 gctcaatggt cgcagcacgc agcgccgtca tttcgtaagc ggcctgttcc agccgttgtt
     5521 gctggcggat gttcagccgg ttacgcatga tatcaaggcc tggataaaga tacggatcgc
     5581 gcccttcgcc gaatttatcg ctcatagtgc ctcctcagct ctacaaggcg cgctaaagcc
     5641 tctgcggcag taagagtgac taaaggcatc tcgacgcctt caagacggcg actggcctgg
     5701 aaattacgat tacgctgaag ctcccagaga cgggactttt gcttatcggt gagtttcttc
     5761 acttgatgcc tccctgaacg tgtccatttg ccacaagtat aagcggcaaa tccgggttac
     5821 gccgggagag cagaagcgcg ggcaagattg cccgcgcgag aaatcattac ggcaggactt
     5881 tagcggaaag gataactacc ggttttgacg gcacattctg gtacggacca acgtcatgag
     5941 tcggcacctg ggaaatctta tcggccacgt ccatgccttt caccacttta ccaaataccg
     6001 cgtaaccgaa atcacgctga ccatggtcaa ggaaggcgtt atcggcaacg ttgataaaga
     6061 actggctggt ggcgctgtct ttgtcagcgg tacgcgccat cgcgatggtg ccacgcgtgt
     6121 tgcgcaggcc gttatcggct tcatttttga ttggcgggtt tggttttttc tgctgcatct
     6181 gctcggtgaa accgccgccc tgaatcataa agccaggaat gacgcggtga aaggtagtgt
     6241 tgttataaaa accgctgttc acataatcga caaagttttg cacagacact ggcgcttttt
     6301 gtttatccag ctccagttcg atgttaccag ctgaggttgt caacaatacg tgcgggtccc
     6361 ctttcgctgc cattgctgcg ggagaaagag cagaaagagc gaaaacagca gccatcgccg
     6421 ccagggtcga tttgaacatg agatttcctt aacaaacaga gcagaaaaaa agcaagtgaa
     6481 gagattctaa atagccttca agaacaaggc catcccttta cctaatttta cgtatctgaa
     6541 atatatgtaa cctgacgatc actttttatt ccgtgatcaa gatcacctct taaaatgcaa
     6601 tttagcaacc gattgcaata aaacatttaa acagatcaca aaatcaccta aaatcacccg
     6661 ctcgcggcat atcccgctat gccattttat ttttgaacgc aggccaatca tgactaacag
     6721 caatcgcatc aagctcacat ggattagctt tctctcctac gcactgactg gtgcgttggt
     6781 tattgtcacc gggatggtga tgggaaatat cgccgattat ttcaatctgc ctgtttccag
     6841 tatgagtaac accttcacct tcctcaacgc cggcatttta atctctatct tcctcaacgc
     6901 ctggctgatg gaaatcgtcc cgttgaaaac gcagttacgt tttggctttc tcctgatggt
     6961 gctggcggtt gccggtttga tgttcagcca cagcctggcg ctgttctcga cggcgatgtt
     7021 cattctcggg gtggtcagcg gcattactat gtcgattggt acattcctga taacacaaat
     7081 gtatgaaggg cgtcagcgcg gttcccgcct gttatttact gactccttct tcagtatggc
     7141 tgggatgatt ttcccaatga tcgccgcgtt tctactggcg cgcagcattg agtggtactg
     7201 ggtttatgcc tgcatcgggc tggtgtatgt cgctattttt attctgacct tcggctgtga
     7261 gttcccagca ctgggcaaac atgcaccaaa aacggatgct ccggtagaga aagaaaagtg
     7321 ggggatcggc gtactgtttc tctctgttgc ggctctgtgc tacatcctcg gtcagttagg
     7381 ttttatctcc tgggtgcctg agtatgccaa aggcctgggc atgagcctga acgacgcggg
     7441 cacgctggtg agtaacttct ggatgtcata catggtcggc atgtgggcgt tcagctttat
     7501 tcttcgcttc tttgatttgc aacgcattct gaccgtactg gctggtctgg ctgcgattct
     7561 gatgtacgtc tttaacaccg gaacaccagc acatatggcg tggtcaattc tcgctctggg
     7621 cttcttctcc agcgcgatct ataccaccat catcaccctg ggttcacagc agaccaaagt
     7681 accgtcgcca aaactggtta actttgtcct gacctgcgga accatcggta ctatgttgac
     7741 ctttgtggtt accggcccga ttgttgaaca tagcggtccg caggcggcac tgcttacagc
     7801 aaacggtctg tacgctgtcg tctttgtgat gtgcttcctg ttaggtttcg tcagccgtca
     7861 ccgtcagcat aacaccctga cctctcatta attgctaatg ccggacggca ctatcgtcgt
     7921 ccggcctttt cctctctaac cccgctacgt gcatctattt ctataaaccc gttcattttg
     7981 tctatttttt gcacaaacat gaaatgtcag acaattccgt gacttaagaa aatttataca
     8041 aatcagcaat atacccctta aggggtatat aaaggtgaat ttgatttaca tcaataagcg
     8101 gggttgctga atcgttaagg taggcggtaa tagaaaagaa atcgaggcaa aaatgagcaa
     8161 agtcagactc gcaattatcg gtaacggtat ggtcggccat cgctttatcg aagatcttct
     8221 tgataaatct gatgcggcca actttgatat taccgttttc tgtgaagaac cgcgcatcgc
     8281 ttatgaccgc gtacacctct cgtcttactt ctctcaccac accgccgaag agctgtcgct
     8341 ggtgcgcgaa ggcttctacg agaaacacgg catcaaagtt ctggtcggcg aacgcgctat
     8401 caccatcaac cgtcaggaga aggtgattca ctccagcgcc ggacgtaccg ttttttatga
     8461 caagctgatc atggcaaccg gttcctaccc gtggatccca ccaatcaaag gttctgatac
     8521 tcaggactgc tttgtctatc gcaccattga agacctcaac gccattgaat cctgcgcccg
     8581 tcgcagcaaa cgcggtgccg ttgttggtgg tggcctgtta ggtctggaag ccgctggcgc
     8641 gctgaaaaac ttaggtattg aaacccacgt tatcgaattt gcccctatgc tgatggcgga
     8701 acagcttgat cagatgggtg gcgagcagct gcgtcgcaaa atcgaaagta tgggcgtgcg
     8761 cgttcacacc agcaaaaaca cccttgagat tgtgcaggaa ggtgtcgaag cacgtaaaac
     8821 catgcgtttt gccgacggca gcgaactgga agtcgacttt atcgtcttct ctaccggtat
     8881 ccgtccgcgc gataagctgg caacccagtg tggtctggac gttgctccgc gtgggggtat
     8941 cgtcattaat gattcctgcc aaacttccga tccggatatc tacgctatcg gtgaatgcgc
     9001 aagctggaac aaccgtgtat ttggtctggt agcacctggc tacaaaatgg cgcaggtcgc
     9061 cgttgaccat attctcggta gcgaaaacgc ctttgaaggt gctgacctta gcgccaagct
     9121 gaaactgctg ggcgtggacg taggcggtat tggtgatgct catggtcgca cgcctggcgc
     9181 acgtagctac gtttacctcg acgaaagcaa agagatctac aaacgcctga tcgtcagcga
     9241 agacaacaaa accctgctcg gtgcggtact ggtgggcgat accagcgact acggtaactt
     9301 gctgcaactg gtgctgaatg ctatcgagct gccggaaaac ccggattctc tgattctgcc
     9361 ggcgcactcg ggtagcggta agccgtctat cggtgttgat aaactgccgg acagcgcgca
     9421 aatctgctcc tgcttcgacg tcactaaagg tgatctgatt gctgccatca acaaaggctg
     9481 ccacacagtt gcggcgctga aagctgaaac caaagcgggt actggctgcg gtggctgtat
     9541 cccgctggtc actcaggtac tgaacgcgga actggcgaaa cagggcatcg aagttaacaa
     9601 caacctgtgc gaacactttg cttattcgcg tcaggaactg ttccatttga tccgcgttga
     9661 aggcattaaa accttcgaag aactgctggc gaaacacggc aaaggctacg gttgtgaagt
     9721 ttgtaaaccg accgtcggtt cgctgctggc ctcctgctgg aacgaataca ttctgaagcc
     9781 agaacatact ccgctgcaag attctaacga caacttcctc gctaacatcc agaaagacgg
     9841 gacctactcg gtgatcccgc gttctccggg cggtgaaatc accccggaag ggctgatggc
     9901 agtgggtcgt atcgcgcgtg aatttaatct ctacaccaaa atcaccggtt cccagcgcct
     9961 ggcgatgttt ggcgcacaga aagacgatct gccagagatc tggcgtcagc tgattgaagc
    10021 cggcttcgaa accggtcatg cctatgcgaa agcactgcgt atggcgaaaa cctgcgtggg
    10081 tagcacctgg tgccgctacg gcgttggcga cagcgtcggc ctcggcgtgg aactggaaaa
    10141 ccgctacaaa ggcatccgta cgccgcacaa aatgaagttc ggtgtctccg gctgtacccg
    10201 tgaatgttca gaagctcagg gtaaagacgt gggtattatc gccactgaaa aaggctggaa
    10261 cctgtatgtt tgcggcaacg gcggcatgaa accgcgtcat gcggatctgc tggcagcgga
    10321 tatcgatcgc gaaacgctga tcaaatatct cgaccgcttc atgatgttct acatccgtac
    10381 cgccgacaaa ctgacgcgta ccgcgccgtg gttagaaaac ctcgaaggcg gcatcgatta
    10441 cctgaaagcg gtgatcattg acgacaaact ggggctgaac gctcatctag aagaagagat
    10501 ggcgcgcctg cgtgaagcgg tagtgtgtga gtggactgaa acggtcaata caccgtccgc
    10561 gcagactcgc ttcaaacact tcatcaacag cgacaagcgt gacccgaacg tgcagatggt
    10621 gccagagcgc gaacagcacc gtccggcaac gccgtatgaa cgtatcccgg taactctggt
    10681 ggaggacaac gcatgagcca gtggaaagac atctgcaaaa tcgatgacat cctgcctgaa
    10741 accggcgtct gcgcgctgtt aggcgacgag caggtcgcga ttttccgccc gtatcacagc
    10801 gaccaggtgt ttgcgatcag caacatcgat ccgttcttcg agtccagcgt gctgtcacgc
    10861 gggctgattg cggaacacca gggcgagctg tgggtcgcca gcccgctgaa aaaacagcgt
    10921 tttcgcttaa gtgatggctt gtgcatggaa gacgaacagt tttccgtcaa acattacgaa
    10981 gcacgagtga aagacggcgt ggtgcagctg cgcggttaat gttttaacgg gaggcgcaat
    11041 gcctcccctt tttgcatggt cctgtaatga ccttcggtat attgcaggac attttttaaa
    11101 ctttttgttt tattttttgt ttttattttt taaaggataa tcaaatgttt acagacacta
    11161 ttaataagtg tgcggctaac gctgcgcgca ttgcacgcct gtcggcaaat aacccgctcg
    11221 gcttttgggt cagctccgcc atggcgggcg cgtatgtggg tcttgggatc atcttgattt
    11281 tcacgctcgg taatttgctc gacccgtccg tacgtccgct ggtgatgggc gcgacctttg
    11341 gtatcgcctt aacgctggtg attatcgccg gttctgaact gttcaccgga cacaccatgt
    11401 tcctcacctt tggggtaaaa gcgggcagca tcagccacgg gcaaatgtgg gcaatcctgc
    11461 cgcaaacctg gctgggtaac ctggtcggtt ccgtcttcgt tgccatgctc tatagctggg
    11521 gcggcggtag cctgctgccg gtagatacca gcatcgttca ctccgtcgcg ctggctaaaa
    11581 ccactgcacc ggcaatggta ctcttcttca aaggtgcatt gtgtaactgg ctggtttgcc
    11641 tggcaatctg gatggcgctg cgcactgaag gggcggcgaa atttatcgct atctggtggt
    11701 gtctgctggc atttatcgcg tccggctacg agcactctat cgctaacatg acgctgttcg
    11761 cgctctcctg gttcggcaac cacagcgaag cctacacgct ggcgggtatt ggtcataacc
    11821 tgctgtgggt gacgctgggt aatactttat caggtgccgt attcatggga ttgggttatt
    11881 ggtatgctac gccgaaatcg aatcgtccgg ttgcggacaa atttaatcaa actgaaacgg
    11941 ctgccggtta attactaagg ggtttttacg tggatcattt gcctatattt tgccaattac
    12001 gcgatcgcga ctgtctgatt gtcggcggtg gtgatgtcgc ggaacgcaaa gcaaggttgc
    12061 tgttagacgc aggcgctcgc ttaacggtga atgcattagc gtttattcca cagttcaccg
    12121 catgggcaga tgcaggcatg ttaaccctcg tcgaagggcc atttgatgaa agccttctcg
    12181 acacctgctg gttggcgatt gcagcgacgg atgatgacac gcttaaccag cgcgtcagcg
    12241 aagccgctga agctcgtcgc atcttctgta acgtagtcga tgcgccgaaa gccgccagct
    12301 ttattatgcc gtcgattatt gaccgctcac cgctaatggt agcggtctcc tctggcggca
    12361 cctctccggt tctggcgcgc ctgttgcgcg aaaaactcga atcactgctg ccgttgcatc
    12421 tgggccaggt ggcgaaatat gccgggcaat tacgcgggcg agtgaaacaa cagttcacca
    12481 cgatgagtga gcgtcgccgt ttctgggaga aattgttcgt taacgaccgc ctggcgcagt
    12541 cgctggcaaa caacgatcag aaagccatta ctgaaacgac cgaacagtta atcaacgaac
    12601 cgctcgacca tcgcggtgaa gtggtgctgg ttggtgcagg tccgggcgat gccgggctgc
    12661 tgacgctgaa aggactgcaa caaattcagc aggcagatgt ggtggtctac gaccgtctgg
    12721 tttctgacga tattatgaat ctggtacgcc gcgatgctga tcgcgttttc gtcggcaaac
    12781 gcgcgggata ccactgtgta ccgcaggaag agattaacca gatcctgctg cgggaagcgc
    12841 aaaaaggcaa acgcgtggtg cggctgaaag gtggcgatcc gtttattttt ggccgtggtg
    12901 gcgaagagct ggaaacactg tgcaacgcgg gtattccgtt ctcggtggtt ccgggtatta
    12961 ccgcagcttc tggttgctct gcctattcgg gtattccact cacgcatcgc gattatgccc
    13021 agagcgtacg cttaattacc ggacacttaa aaaccggtgg cgagctggac tgggaaaacc
    13081 tggcggcaga aaaacagacg ctggtgttct atatggggtt gaatcaggcc gcgactattc
    13141 agcaaaagct gattgaacac ggtatgcctg gcgaaatgcc ggtggcaatt gtcgaaaacg
    13201 gaacggcagt cacgcagcgc gtgattgacg gtacgctcac gcaactcggt gaacttgctc
    13261 agcaaatgaa cagtccatcg ctaattatta ttggtcgggt tgttggcctg cgcgataaac
    13321 tgaactggtt ctccaaccat taatttaacc cgggccagag aattctggcc ttcttaatga
    13381 ctctttt
//
LOCUS       DAJTMK010000110        13215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTMK010000110 DAJTMK010000000
VERSION     DAJTMK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.111"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            7..82
                     /locus_tag="OO135_002744"
     tRNA            7..82
                     /locus_tag="OO135_002744"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Trp,seq:cca)
     gene            complement(178..1017)
                     /gene="hdfR"
                     /locus_tag="OO135_002745"
     CDS             complement(178..1017)
                     /gene="hdfR"
                     /locus_tag="OO135_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5398538.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDNTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            1136..1474
                     /gene="maoP"
                     /locus_tag="OO135_002746"
     CDS             1136..1474
                     /gene="maoP"
                     /locus_tag="OO135_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5398539.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            complement(1498..1765)
                     /locus_tag="OO135_002747"
                     /pseudo
     CDS             complement(1498..1765)
                     /locus_tag="OO135_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
     gene            complement(1762..2922)
                     /locus_tag="OO135_002748"
     CDS             complement(1762..2922)
                     /locus_tag="OO135_002748"
                     /inference="COORDINATES: protein motif:HMM:NF007365.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCR5398540.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTKVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYGLVGELALTGALRGVPGAISSTTEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVIGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFHSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCVISTDSLVPFSTASISHWRSHYRLPVF"
     gene            3273..3371
                     /gene="ilvL"
                     /locus_tag="OO135_002749"
     CDS             3273..3371
                     /gene="ilvL"
                     /locus_tag="OO135_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5398541.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            3458..3508
                     /locus_tag="OO135_002750"
     CDS             3458..3508
                     /locus_tag="OO135_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398542.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            3511..5157
                     /locus_tag="OO135_002751"
     CDS             3511..5157
                     /locus_tag="OO135_002751"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5398543.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            5154..5417
                     /gene="ilvM"
                     /locus_tag="OO135_002752"
     CDS             5154..5417
                     /gene="ilvM"
                     /locus_tag="OO135_002752"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5398544.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            5437..6366
                     /gene="ilvE"
                     /locus_tag="OO135_002753"
     CDS             5437..6366
                     /gene="ilvE"
                     /locus_tag="OO135_002753"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5398545.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            6431..8281
                     /gene="ilvD"
                     /locus_tag="OO135_002754"
     CDS             6431..8281
                     /gene="ilvD"
                     /locus_tag="OO135_002754"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5398546.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            8284..9828
                     /gene="ilvA"
                     /locus_tag="OO135_002755"
     CDS             8284..9828
                     /gene="ilvA"
                     /locus_tag="OO135_002755"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5398547.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(9825..10715)
                     /gene="ilvY"
                     /locus_tag="OO135_002756"
     CDS             complement(9825..10715)
                     /gene="ilvY"
                     /locus_tag="OO135_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5398548.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            10865..12340
                     /gene="ilvC"
                     /locus_tag="OO135_002757"
     CDS             10865..12340
                     /gene="ilvC"
                     /locus_tag="OO135_002757"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5398549.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            complement(12427..12708)
                     /gene="ppiC"
                     /locus_tag="OO135_002758"
     CDS             complement(12427..12708)
                     /gene="ppiC"
                     /locus_tag="OO135_002758"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5398550.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(12907..>13215)
                     /locus_tag="OO135_002759"
     CDS             complement(12907..>13215)
                     /locus_tag="OO135_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5398551.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINTISLIN
                     "
BASE COUNT         3157 a         3422 c         3576 g         3060 t
ORIGIN      
        1 ctaattaggg gcgtagttca attggtagag caccggtctc caaaaccggg tgttgggagt
       61 tcgagtctct ccgcccctgc cagaaatcat ccttagcgaa agctaaggat tttttttatc
      121 tgaaataacc ctctccgaag taaatccttc taccggcatc cttgccagcc attcatatta
      181 atacacttca tccagcacgt taattttcaa aagatcgcga atcaacgcat ttttatcgct
      241 attttgcagc catatggcat aaagcggccg tgaaagtgtt gtgttatcga caacggtatg
      301 caggccgcct tttttacgcg cccagctgac gggtagccag gtgcaaccat taagcatcgc
      361 aatctgttgc tgtgccagtt cagcagaact ggttgtcaga atgggcactt cgtcagcacc
      421 gatcaaacct gcctcatgct gttgaaaatc tggcccccac tcaagtcgca gataattaag
      481 atctcccttt agttttgaag gggcactggt ataaagcgct aaagtgaaat atcccagcaa
      541 ctgactacta aattcgtcca ttttgggcgc ttcagtggta ataagaagat caagctggcg
      601 ttcatgcagc tgttttacca gagactgccg ttgggcaatt cgcgcttcga actgtaagcc
      661 tgtatgggca tcctgatttt gatacaaacg tcccagccac tgattaagca tacattccca
      721 caatgaggcg ctggcaccga tagaaaactc gttatgtcgt gaggtatgcg ccacctcctt
      781 gcgggcggcc tgccaggtgc tcatgagcgt ttctgcataa ggcagtagtt tttcaccggc
      841 agcggttaaa cggatattgt ttctgtggcg ggtgaaaagg ttcacaccca gttgattttc
      901 cagttgtctg attcgaaagc tcactgctga ctgggtcaga tagagcgatt cagccgctcg
      961 accaaagtga cgcgttcggc taacttccag gaaagttttt aacaattccg tatccacagt
     1021 gctctccgca aaattatttg ttgttatgat ttaaatgttt tgttttacac tctgtcaagc
     1081 gtaactaata ctccgcgcca taactagctc ggtcaaagaa ttaggagcgt gcaggatggc
     1141 ggaaagcttt acgacgacta atcgatattt cgacaataaa cattatccac gtggattctc
     1201 tcgtcatggt gatttcacca tcaaagaggc acaactgctt gagcgtcatg gttatgcctt
     1261 caatgagttg gatcttggca aacgcgagcc ggttaccgag gaagagaaac tcttcgtagc
     1321 agtatgccgt ggcgaacgtg agccagtgac agaagcagaa cgcgtgtggt ccaagtatat
     1381 gacgcgtatt aagcgtccaa aacgttttca caccctttcc ggcggtaaac cgcaggttga
     1441 aggtgctgaa gactacaccg attctgacga ttaataaaaa gggcgaaatg ccctttttta
     1501 tgtcagtagt ttctgcagat ggatgagcaa acggtcaatc gctcgatagc taactgcctc
     1561 ctgcaaatgt tgacgtgtga taatgtcaga ctgatcaata tcagcaatgg ttcgtgcaac
     1621 tttcaataac cgctgccagg cacgaatcga taaccccaga tggatcagcg tttcttccag
     1681 ccacatcgca tcttcgctct caggcggcca ttacgcgttg tttaacggtg gcgctgcttt
     1741 ctcccggcac taccgtttta ctcaaaatac cgggaggcgg taatgggatc tccagtgaga
     1801 gatcgaagcg gtcgagaaag ggaccagaga gtcggttgag ataacgcagc ggttatggtt
     1861 tccctgataa tgtccggtag ggctgggatt catcgccgcg acaagctgga aacgggctgg
     1921 ataggttatt tttgctcgcg tgcgtgaaag atggatctgc ccggattcaa tcggctctcg
     1981 caaggcatcc agtgtacgcc tttcaaattc aggtaactca tcaagaaaaa gcacgccgtt
     2041 atgcgccagc gaaatttcac cgggccctgg aattgcgcca ccgcctacca tcgcagttaa
     2101 cgatgcactg tgatgaggtg agtggaacgg gcgctgccgc cattgttttt gtactgattc
     2161 agcatttacc agacttaata tcgcagcact ctccagtgcc tcttcattgc ttaaatctgg
     2221 caacaggcca ttaatacggc tggcgagcat tgttttacct gttcccggcg gcccaatcag
     2281 taaaaggttg tgcccgccag cggcggtaat ttccagtcct cgctttcctt gttcctgacc
     2341 gataacatca ctgagatcat gttgtagcgc ccgggatact gcatcagttg gtttcgggcg
     2401 ttcgagagcg tgcttacctt ccagaaacgc acagacagct tgcaggtgat cggctatcag
     2461 gcatccttca ccgttaatta gccccacttc atcttcgtta tctttcgcga cgataatttt
     2521 tctgcccgac ttaatagctt cagttgtact ggagattgcg ccgggaacgc cacgcagagc
     2581 gcctgtaagc gccagttctc cgactaatcc atattcatct aacttattgg ctgtaagctg
     2641 ttctgaggcc gccagcaacg caatggcgat aggtaaatca tatcgtcccc cttcttttgg
     2701 cagatcagct ggagccaggt tgatggtgat ttttttcgcc ggatattcat atccgctatt
     2761 gataatggcg ctgcgcacgc gatcgcgagc ttcttttacc gttgtttctg gtaagcccac
     2821 cttcgttaag ccgggtagac ctttactgat atgtacctca acagtgatcg ggggcgcatt
     2881 tactcccagg gctgcgcggg tatgaacaat tgacagtgac ataagccctc cttgagtcac
     2941 cattatgtgc ataagatatc gctgctgtag cccgctaatt cgtgaatttt agtggctgat
     3001 tcctgtttat ttgtgcaagt gaagttgagt tgttctggcg gtggaatgat gctcgcaaaa
     3061 atgcagcgga caaaggatga actacgagga agggaacaac attcatactg aaattgaatt
     3121 tttttactca ctattttatt tttaaaaaaa acaacttata ttgaaattat taaacgcatc
     3181 ataaaaatcg gccaaaaaat atcttgtact atttacaaaa cctatggtaa ttctttaggc
     3241 attccttcga acaagatgca agaaaagaca aaatgacagc ccttctacga gtgattagcc
     3301 tggtcgtgat tagcgtggtg gtgattatta tcccaccgtg cggggctgca cttggacgag
     3361 gaaaggctta gaaatcaagc cttaacgaac taagaccccc gcaccgaaag gtccgggggt
     3421 tttttttgac cttaaaaaca taaccgagga gcagacaatg aataacagca caaaattctg
     3481 tttctcaaca ttcagcacgg ggaactaact atgaatggcg cacagtgggt ggtacatgcg
     3541 ttgcgggcac agggtgtgaa caccgttttc ggttatccgg gtggcgcaat tatgccggtt
     3601 tacgatgcat tgtatgacgg cggcgtggag cacttgctgt gccgacatga gcagggtgcg
     3661 gcaatggcgg ctatcggtta tgcccgtgct accggcaaaa ctggcgtatg tatcgccacg
     3721 tctggtccgg gcgcaaccaa cctgataacc gggcttgcgg acgcactgtt agattctatc
     3781 cctgttgttg ccatcaccgg tcaagtgtcc gcaccgttta tcggcactga tgcatttcag
     3841 gaagtggatg tcctgggatt gtcgttagcc tgtaccaagc acagctttct ggtgcagtcg
     3901 ctggaagagt tgccgcgcat tatggctgaa gcattcgacg ttgccagctc aggtcgtcct
     3961 ggtccggttc tggtcgatat cccaaaagat atccagctag ccagcggtga cctggaaccg
     4021 tggttcacca ccgttgaaaa cgaagtgact ttcccacatg ccgaagttga gcaagcgcgc
     4081 cagatgctgg caaaagcgca aaaaccgatg ctgtacgttg gtggtggcgt gggtatggcg
     4141 caggcagttc ctgctttacg agaatttctc gctaccacaa aaatgcctgc cacctgcacg
     4201 ctgaaagggc tgggcgcagt tgaagcagat tatccgtact atctgggcat gctgggaatg
     4261 catggcacca aagcggcgaa cttcgcggtg caggagtgcg acttgctgat cgccgtgggt
     4321 gcacgttttg atgaccgggt gaccggcaaa ctgaacacct tcgcaccaca cgccagcgtt
     4381 atccatatgg atatcgaccc ggcagaaatg aacaagctgc gtcaggcaca tgtggcatta
     4441 caaggtgatt taaatgctct gttaccagaa ttacagcagc cgttaaatat caatgactgg
     4501 cagcaatact gcgcgcagct gcgtgatgaa catgcctggc gttacgacca tcccggcgac
     4561 gctatctacg cgccgttgtt gttaaaacaa ctgtcggatc gtaaacctgc ggattgcgtc
     4621 gtgaccacag atgtggggca gcaccagatg tgggctgcgc aacacatcgc ccacactcgc
     4681 ccggaaaatt tcatcacctc cagcggctta ggcactatgg gttttggttt accagcggcg
     4741 gttggcgcac aagtcgcgcg accgaacgat accgtcgtct gtatctccgg tgacggctct
     4801 ttcatgatga atgtgcaaga gctgggcacc gtaaaacgca agcagttacc gttgaaaatc
     4861 gtcttactcg ataaccaacg gttagggatg gttcgacaat ggcagcaact gttttttcag
     4921 gaacgataca gcgaaaccac ccttactgat aaccccgatt tcctcatgtt agccagcgcc
     4981 ttcggcatcc ctggccaaca catcacccgt aaagaccagg ttgaagcggc actcgacact
     5041 atgctgaaca gtgatgggcc atacctgctt catgtctcaa tcgacgaact tgagaacgtc
     5101 tggccgctgg tgccgcctgg cgccagtaat tcagaaatgt tggagaaatt atcatgatgc
     5161 aacatcaggt caatgtatcg gctcgcttca atccggaaac cttagaacgt gttttacgcg
     5221 tggtgcgtca tcgtggtttc cacgtctgct caatgaatat ggccgccgcc agcgatgcac
     5281 aaaatataaa tatcgaattg accgttgcca gcccacggtc ggtcgactta ctgtttagtc
     5341 agttaaataa actggtggac gtcgcacacg ttgccatctg ccagagcaca accacatcac
     5401 aacaaatccg cgcctgagcg caaaaggaat ataaaaatga ccacgaagaa agctgattac
     5461 atttggttca atggggagat ggttcgctgg gaagacgcga aggtgcatgt gatgtcgcac
     5521 gcgctgcact atggcacttc ggtttttgaa ggcatccgtt gctacgactc gcacaaagga
     5581 ccggttgtat tccgccatcg tgagcatatg cagcgtctgc atgactccgc caaaatctat
     5641 cgcttcccgg tttcgcagag cattgatgag ctgatggaag cttgtcgtga cgtgatccgc
     5701 aaaaataatc tcaccagcgc ctatatccgt ccgctgatct tcgtcggtga tgttggcatg
     5761 ggagtaaacc cgccagcggg atactcaacc gacgtgatta tcgctgcttt cccgtgggga
     5821 gcgtatctgg gcgcagaagc gctggagcag gggatcgatg cgatggtttc ctcctggaac
     5881 cgcgcagcac caaacaccat cccgacggcg gcaaaagccg gtggtaacta cctctcttcc
     5941 ctgctggtgg gtagcgaagc gcgccgccac ggttatcagg aaggtatcgc gctggatgtg
     6001 aacggttaca tctctgaagg cgcaggtgaa aacctgtttg aagtgaaaga cggcgtgctg
     6061 ttcaccccac cgtttacctc atccgcgctg ccgggtatta cccgtgatgc catcatcaaa
     6121 ctggcgaaag agctgggaat tgaagtgcgt gagcaggtgc tgtcgcgcga atccctgtac
     6181 ctggcggatg aagtgtttat gtccggtact gcggcagaaa tcacgccagt gcgcagcgta
     6241 gacggtattc aggttggcga aggccgttgt ggcccggtta ccaaacgcat tcagcaagcc
     6301 ttcttcggcc tcttcactgg cgaaaccgaa gataaatggg gctggttaga tcaagttaat
     6361 caataaatac aaaaaatggg acggcacgca ccgtcccatt tacgagacag acactgggag
     6421 taaataaagt atgcctaagt accgttccgc caccaccact catggtcgta atatggcggg
     6481 tgctcgtgcg ctgtggcgcg ccaccggaat gaccgacgcc gattttggca agccgattat
     6541 cgcggttgtg aactcgttca cccaatttgt accgggtcac gtccatctgc gcgatctcgg
     6601 taaactggtc gccgaacaaa ttgaagcggc tggcggcgtt gccaaagagt tcaacaccat
     6661 tgcggtggat gatgggattg ccatgggcca cggggggatg ctttattcac ttccatctcg
     6721 cgaactgatc gctgattccg ttgagtatat ggtcaacgcc cactgcgccg acgccatggt
     6781 ctgcatctct aactgcgaca aaatcacccc ggggatgctg atggcttccc tgcgcctgaa
     6841 tattccggtg atctttgttt ccggcggccc gatggaggcc gggaaaacca aactgtccga
     6901 tcagataatc aaactcgatc tggttgatgc gatgatccag ggcgcagacc cgaaagtttc
     6961 tgactcccag agcgatcagg ttgaacgttc cgcctgccca acctgcggtt cctgctccgg
     7021 gatgtttacc gctaactcaa tgaactgcct gaccgaagcg ctgggtttgt cgcagccagg
     7081 caacggctcg ctgctggcaa cccacgcgga ccgtaagcag ctgttcctta atgctggtaa
     7141 acgcattgtt gaattgacca aacgttatta cgagcaaaac gacgaaagtg cactgccgcg
     7201 taatatcgcc agtaaggcgg cgtttgaaaa cgccatgacg ctggatatcg cgatgggtgg
     7261 atcgactaac accgtacttc acctgctggc ggcggcgcag gaagcggaaa tcgacttcac
     7321 catgagtgat atcgataagc tttcccgcaa ggttccacag ctgtgtaaag ttgcgccgag
     7381 cacccagaaa taccatatgg aagatgttca ccgtgctggt ggtgttatcg gtattctcgg
     7441 cgaactggat cgcgcggggt tactgaaccg tgatgtgaaa aacgtgctcg gcctgacgtt
     7501 gccgcaaacg ctggagcaat acgacgttat gctgacccag gatgacgcgg taaaaaatat
     7561 gttccgcgcc ggccctgcgg gtatccgtac cacccaggca ttctcgcaag attgccgttg
     7621 ggatacgctg gacgacgatc gcgcaaacgg ctgtatccgc tcgctggaac acgcctacag
     7681 caaagacggc ggcctggcgg tgctctacgg taattttgct gaaaacggct gcatcgtgaa
     7741 aaccgcgggc gtcgatgaca gcatcctcaa attcaccggc ccggcaaaag tgtacgaaag
     7801 ccaggacgac gcggtagaag cgatcctcgg tggcaaagtt gtcgcgggcg atgtggtggt
     7861 gatccgctat gaaggcccga aaggcggccc tggcatgcag gaaatgctct acccaaccag
     7921 cttcctgaaa tcaatgggtc tcggtaaagc ctgtgcgctg atcaccgacg gtcgtttctc
     7981 tggcggcacc tctggtcttt ccatcggcca cgtctcaccg gaagcggcaa gcggcggcag
     8041 cattggtctg attgaagatg gtgacctgat cgctatcgac atcccgaacc gtggcattca
     8101 gttacaggta agcgatgccg aactggcggc gcgtcgtgaa gcgcaggaag ctcggggtga
     8161 caaagcttgg acgccgaaaa accgtgaacg tcaggtttcc tttgccctgc gcgcctacgc
     8221 cagcctggca accagcgccg acaaaggtgc ggtgcgcgat aaatcgaaac tggggggtta
     8281 ataatggctg actcgcaacc cctgtccggt gctccggaag gtgctgaata tttaagagca
     8341 gtgctgcgcg cgccggttta cgaggcagcg caggttacgc cgctacaaaa aatggaaaaa
     8401 ctgtcgtcgc gtcttgataa cgtcattctg gtgaagcgcg aagatcgcca gccagtgcac
     8461 agctttaagc tgcgcggcgc atacgccatg atggcgggcc tgacggaaga acaaaaagcg
     8521 cacggcgtga tcactgcttc tgcgggtaac cacgcgcagg gcgtcgcgtt ttcttctgcg
     8581 cggttaggcg tgaaggccct gatcgttatg ccaaccgcca ccgccgacat caaagtcgat
     8641 gcggtacgtg gattcggcgg cgaagtgctg cttcatggcg cgaactttga cgaagcgaaa
     8701 gctaaagcga tcgaactgtc acagcagcag gggttcacct gggtgccgcc gttcgaccat
     8761 ccgatggtga ttgccgggca aggcacgctg gcgctggaac tgctccagca ggacgcccat
     8821 ctcgaccgcg tatttgtgcc agtcggcggc ggcggtctgg cggcgggcgt tgcggtgctg
     8881 atcaaacaac tgatgccaca aatcaaagtg atcgccgtag aagcggaaga ctccgcctgc
     8941 ctgaaagcag cgctggatgc gggtcatccg gttgatctgc cgcgcgtagg gctatttgct
     9001 gaaggcgtag cggtaaaacg catcggtgac gaaaccttcc gtttatgcca ggagtatctc
     9061 gacgacatca tcaccgtcga tagtgatgcg atctgtgcgg cgatgaagga tttattcgaa
     9121 gatgtgcgcg cggtggcgga accctctggc gcgctggcgc tggcgggaat gaaaaaatat
     9181 atcgcccagc acaacattcg cggcgaacgg ctggcgcata ttctttccgg tgccaacgtg
     9241 aacttccacg gcctgcgcta cgtctcggaa cgctgcgaac tgggcgaaca gcgtgaagcg
     9301 ttgttggcgg tgaccattcc ggaagaaaaa ggcagcttcc tcaaattctg ccaactgctt
     9361 ggcgggcgag cggtcaccga gttcaactac cgttttgccg atgccaaaaa cgcctgcatc
     9421 tttgtcggcg tgcgcctgag ccgcggcctc gaagagcgca aagaaatttt gcagatgctc
     9481 aacgacggcg gctacagcgt ggtggatctc tccgacgacg aaatggcgaa gttgcatgtg
     9541 cgctatatgg tcggcgggcg tccgtcacat ccgttgcagg aacgcctcta cagcttcgaa
     9601 ttcccggaat caccgggcgc gctgctgcgc ttcctcaaca cgctgggtac gcactggaac
     9661 atttctttgt tccactatcg cagccatggc accgactacg ggcgcgtact ggcggcgttc
     9721 gaactcggcg accatgaacc ggatttcgaa acccggctaa atgagctggg ctacgattgc
     9781 cacgacgaaa ccaataatcc ggcgttcagg ttctttttgg cgggttaatg gttcggcaaa
     9841 atcttccaga acgcctcaat cagcggctca tgcagccgct ttttctgcgc acaaacgccc
     9901 agttcaaacg gcgttttttc atcgctgcgc tctaaaatca tcacgcggtt acgcaccggt
     9961 tcggggctgt tttccagcac tacttccggc aacagcgcca cgccacagcc gagcgccacc
    10021 atcgatacca tcgcttcatg cccgccaacc gtggcgtaaa tcatcgggtt gctgatttta
    10081 ttgcgtcgaa accacagttc aatgcggcgg cgtaccggtc cctgatcggc catgataaac
    10141 ggcactgtag accagtccgg cttctctgac gaaacctggt tacgcaccgg gcagggcagc
    10201 gcgggcgcaa tcagcactac tgccagattc tccagcatcg aaaacgccac tgcgccgggc
    10261 agagtctccg gtttcccggc aatcgccaga tccgcttcgc cagtgactac cttttccatc
    10321 gcatctgccg catcaccagt ggttagtttg atctccaccg acgggtgttc cgcgcggaag
    10381 cggtcgagaa tcggtggcag atggctgtag gcagcggtca ccgagcagaa gatatgtaat
    10441 tcgccagaga gcgacggccc ttgctgatcg atggtgtggc gcaactgctg atactgcaac
    10501 agcgtttgct gggcgaaaac gcgcagctct tcgcccgctt cagtcagcgt caccgtgcgg
    10561 ttatcgcgca caaatagcgg ctgaccaaga tcttcttcca ggcgctgaat ctgccgtgag
    10621 agcgtggatg ggctaacgtg catcgcccgc gcgctgcggc caaaatggcg gctttccgcc
    10681 agatgcagga aggttttcag atcgcgtaaa tccaatggag cagccctcaa tgttgacgtt
    10741 gcagaaattg caatgtgacg ttgtgaatat atcaatttcc gcaataaatt tcctgtcata
    10801 tagtgaattc aatctcgcaa acgcgaaccg aacaataaga agcacaacat cacgaggaat
    10861 caccatggct aactacttca atacactgaa cctgcgccag cagctggcac agctgggcaa
    10921 atgtcgcttt atggggcgcg acgaattcgc cgacggcgcg agctaccttc agggtaaaaa
    10981 agtggttatc gtcggctgtg gcgcacaggg gctgaaccag ggcctgaaca tgcgtgattc
    11041 tggtctcgat atctcctacg ctctgcgtaa agaagcgatt gccgagaagc gcgcatcctg
    11101 gcgtaaagcg accgaaaacg gttttaaagt gggcacttac gaagaactga tcccgcaggc
    11161 ggatctggtg gttaacctga cgccagacaa gcagcactcc gacgtagtgc gcaccgtaca
    11221 gccactgatg aaagacggcg cggcgctggg ttactcgcac ggtttcaaca tcgtcgaagt
    11281 gggcgagcag atccgtaaag acatcaccgt agtcatggtt gcgccgaaat gccctggcac
    11341 cgaagtgcgt gaagagtaca aacgtggatt cggcgtaccg acgctgattg ccgttcaccc
    11401 ggaaaacgat ccgaaaggcg aaggcatggc gatcgctaaa gcatgggcgg cagcaaccgg
    11461 tggtcaccgt gcgggcgtgc tggaatcgtc cttcgttgcg gaagtgaaat ctgacctgat
    11521 gggtgagcaa accatcctgt gcggtatgtt gcaggctggc tcgctgctgt gcttcgacaa
    11581 gctggtggaa gagggcaccg atccggcata cgcagaaaaa ctaattcagt tcggttggga
    11641 aaccatcacc gaagcgctga agcagggcgg catcaccctg atgatggacc gtctctctaa
    11701 cccggcgaaa ctgcgtgctt acgcgctgtc tgaacagctg aaagagatca tggcacccct
    11761 gttccagaaa catatggacg acatcatctc cggcgaattc tcctccggta tgatggcgga
    11821 ctgggccaac gatgataaga aactgctgac ctggcgtgaa gagaccggca aaaccgcctt
    11881 cgaaaccgcg ccgcagtatg aaggcaaaat cggcgagcag gagtacttcg ataaaggcgt
    11941 actgatgatc gcgatggtga aagcgggcgt tgagctggcg tttgaaacca tggtcgattc
    12001 cggcatcatt gaagagtctg catattatga atcactgcac gagctgccgc tgattgccaa
    12061 caccatcgcc cgtaagcgtc tgtacgaaat gaacgtggtt atctctgata ccgctgagta
    12121 cggtaactat ctgttctctt acgcttgtgt gccgttgctg aaaccgttta tggcagagct
    12181 gcaaccgggc gatctgggta aagctattcc ggaaggtgcg gtagataacg cgcaactgcg
    12241 tgatgtgaac gaagcgattc gctgccatgc gattgagcag gtaggtaaga aactgcgcgg
    12301 ctatatgacg gatatgaaac gtattgcagt tgcgggttaa gtgcgcgctg atgccctcac
    12361 cccgaccctc tcccacaggg agagggagaa aacactcaag gccttctcct ggagaaggcc
    12421 ttgctattag ttacggtaca gcaccttaat gatgtggtaa ccgaactggg tgtgcagcgg
    12481 gccggtcggc tccagtaccg gacaagagaa cacaacttta tcgaacgccg gaaccatctg
    12541 accctggcgg aattcaccta aatcaccgcc gcgtttgcct gatgggcaaa tggagtgttt
    12601 cttcgccagc ttgccgaaat cggcaccgtt cttaatctgc tcgagaagat ccagagccag
    12661 tttctcttct tttacaagga tatgcagtgc tgctgctgtt tttgccatga tcgtgccttg
    12721 agtgaataat tgtcaggcgg cgattttagc atgatccgca gatgctaacg taattgtcag
    12781 tcagggaggg gctggtacat atatggcaaa agcccggaaa atccgggctt cgcaggtccc
    12841 tcaaggggag aaaacttagg gcctctatgg gctacaaagt cattatcggg gatggttatt
    12901 ttattgtcaa tttatgagcg atatggtatt gattgacttg atgaccgccc tctgtatgtc
    12961 tgcaaccaac tcatgtggca aacattgatt aagataccct ctgtaagtcc ttgctctatt
    13021 taatcggttt cgactaactt gttgtaccag gtcggctttg gcccaacgaa tctgctgatc
    13081 aaagaattgc aggccattga taacattact ggctatttca acatgcattc cacgatttaa
    13141 cttgtcatga tcgcgagtgg ttgataatgg aacgacgata catgcgttgc cattaatttt
    13201 tccgttgagc acgac
//
LOCUS       DAJTMK010000111        13153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTMK010000111 DAJTMK010000000
VERSION     DAJTMK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.112"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..897
                     /locus_tag="OO135_002760"
     CDS             <1..897
                     /locus_tag="OO135_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR5398552.1"
                     /translation="IDGRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPG
                     RQAYRELRLLAGQAARQQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVP
                     RKYHLIRTAYEINEEPTAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAA
                     ADQGACAPWTRGNNCPLAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKER
                     RELAARLRLVKPKRRKDYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSG
                     AGLRIFYRNQRLQEDDQWRNLY"
     gene            1051..1239
                     /locus_tag="OO135_002761"
     CDS             1051..1239
                     /locus_tag="OO135_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398553.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            1250..1483
                     /locus_tag="OO135_002762"
     CDS             1250..1483
                     /locus_tag="OO135_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR5398554.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            1776..3425
                     /locus_tag="OO135_002763"
     CDS             1776..3425
                     /locus_tag="OO135_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5398555.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            3409..4290
                     /locus_tag="OO135_002764"
     CDS             3409..4290
                     /locus_tag="OO135_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398556.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(4398..5429)
                     /locus_tag="OO135_002765"
                     /pseudo
     CDS             complement(4398..5429)
                     /locus_tag="OO135_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520363.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(5429..7195)
                     /locus_tag="OO135_002766"
     CDS             complement(5429..7195)
                     /locus_tag="OO135_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCR5398557.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVYSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKDIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKNFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQAYAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7338..8171
                     /locus_tag="OO135_002767"
     CDS             7338..8171
                     /locus_tag="OO135_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCR5398558.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            8188..9246
                     /locus_tag="OO135_002768"
     CDS             8188..9246
                     /locus_tag="OO135_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCR5398559.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            9250..9900
                     /locus_tag="OO135_002769"
     CDS             9250..9900
                     /locus_tag="OO135_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCR5398560.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IWRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            9996..10442
                     /locus_tag="OO135_002770"
                     /pseudo
     CDS             9996..10442
                     /locus_tag="OO135_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
     gene            10442..10645
                     /locus_tag="OO135_002771"
     CDS             10442..10645
                     /locus_tag="OO135_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5398561.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFVP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            10649..10864
                     /locus_tag="OO135_002772"
     CDS             10649..10864
                     /locus_tag="OO135_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398562.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            10845..11357
                     /locus_tag="OO135_002773"
     CDS             10845..11357
                     /locus_tag="OO135_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5398563.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            11359..11736
                     /locus_tag="OO135_002774"
     CDS             11359..11736
                     /locus_tag="OO135_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398564.1"
                     /translation="MKKKLISGLFLMLWMALLIAAIVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            11733..12161
                     /locus_tag="OO135_002775"
     CDS             11733..12161
                     /locus_tag="OO135_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCR5398565.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            12257..12688
                     /locus_tag="OO135_002776"
     CDS             12257..12688
                     /locus_tag="OO135_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5398566.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            12681..13082
                     /locus_tag="OO135_002777"
     CDS             12681..13082
                     /locus_tag="OO135_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5398567.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKTAASADSASVQFEGRVQRIA
                     RIHHYGLSDRVSRKGIYTIYAKRQLLGFNVR"
BASE COUNT         3373 a         3000 c         3563 g         3217 t
ORIGIN      
        1 attgacgggc gtggtctggc tggcgagatc agcaaggaaa cgggtaaatc cctgcgtgat
       61 aacgctgaat acgttaatcc ctgggcgtct ctgcatcgtg ttcagcaatt ccgcttcttt
      121 ggtattccag ggcgtcaggc ttaccgtgaa ctgcggttgt tggctggtca ggcggcaagg
      181 cagcagggtg acaaaaaagc aggtgcgccg gtactggata acccgcgcct tgatgccatc
      241 ctggctgctg ctgatgctgg ctgttttgcc acctacatta tgaggcaggg cggcgtgctg
      301 gttcctcgca aatatcactt gatcagaacc gcttatgaaa tcaatgaaga gccgaccgcc
      361 tatggcgatc acggtattcg tatttatggc atctggtcac ccattgcaga aggcaagatc
      421 tgcactcatg cagtgaagtg gaaaatggtt cgtaaagccg ttgacgttca ggaggcggca
      481 gccgaccagg gcgcttgcgc cccttggact cgtggcaata actgtcccct tgctgaaaat
      541 ttgaaccaac aggagaaaga taaatcagct gatgggaacc ccagaacgga cattaccagc
      601 atggatgaca aggagttgca cgattacctg cacagtatga gcaaaaaaga gcgccgggaa
      661 ctggcagcaa ggttacgcct ggtgaaaccg aaacggcgta aagactacaa acagcgaatt
      721 acagaccatc agcgactgca gctcgtgtat gagctgaagt ccagaggatt tgatggcagc
      781 gagaaagagg tcgatttact ccttcgcgga ggcagtattc cgtcaggagc aggcctgcgt
      841 atcttctatc ggaaccagcg tttgcaggaa gatgatcagt ggcggaacct gtattaatta
      901 cgcgggttaa caattcgtgc tcttaataat accaggcata tcaggctgat gaacgtaaaa
      961 aaacgtttta catcagtaag attattatat actgtaaata taaacagtgg ttatgcatgc
     1021 agtattgcgt gtggtgtcat aggaggaaag atgcaggact attttttgga gtctttgaag
     1081 ctccagcgca ttgatttttt tcttaagctt gtagcggcta gtgagtgtag tgatgaagag
     1141 aaggggctgg ctctgcagtg ggtttctgaa ttgactgatg aactcatggc aaaaatcaga
     1201 acccacgaat acaaccgctc aatggatgtc atcagctgag gtgactttta tgcgcattga
     1261 aataatgatc gataaagagc agaagattag ccagtctacc ctggacgctc ttgaatccga
     1321 gctttaccgc aatctgcgcc ctctgtatcc caaaacggta attcgtatcc gcaaaggtag
     1381 ctctaacggt gtggaactta ccggattgca actggacgaa gaaagaaaac aagtgatgaa
     1441 aattatgcag aaggtgtggg aagacgacag ctggctgcat taagaaacgt tgctggcgtc
     1501 tgaactagct tctggcgtca gcaaggttga acaacgagcc cttgcgaggc gttagctctg
     1561 tagtgcatgt ctatgccgca tgagatcgca tgatcgtttg aggatcgttt ttgctaaggc
     1621 ccgccagaac tggcgggctt ttgcgtagat catgcaggtg catgaaaacc actacataaa
     1681 gcgggcaggc gtggcgggga tacgattgcg cgctaggtat aagtgtgtaa aataattcca
     1741 aaataaatca tcactaatga agtgagaggg taataatgtt agtcaagaaa aataaaaatt
     1801 atagtttata ttttttaatt acaaataatc aacagatatc tgattctggt aattatataa
     1861 aaatagtccc atcaagaagc gattggaatg attttggata tttgagtaga gtcgaaatat
     1921 ctatttttaa taaaaaagag tgtaaggata taatttcact gaatggttat attggttttt
     1981 tgaattcaga ggaggaacga aatggaaaag cagaactgat aaggcgagtg aaaaaaaagg
     2041 aagaatctaa tttaaaggat gaggggttta atgattattt tgtcatgctt caggatatga
     2101 ataattatcg aagactagta agattttttg gggtgaatga atccaaatat atattaaatt
     2161 tcattaatga tgttgtttct aagaaaagtg acgctagtgc agagaatttg aggaaaaaag
     2221 caatctcttc tgatatcttt aataaatctt tcattagaga gtctgagtcg tattttactt
     2281 tcaaaaatgc aggaacagtt ctttccggta ttcaatatga ggagttgggt aatttatcac
     2341 agcacatcaa gattagctat tcgaataaag ttaatgaaga agatgtatct tatgttttta
     2401 attttgatca cgagcatgat ttaccaaaaa gaatttccat aattataggc gagaatggtg
     2461 taggaaaaag ccaaacgctt agagaaatcg cattggctgc gattaaagga aaggataatt
     2521 tggtggctgt agttgattct aacggtgtga gcattgaaga acgaattcaa attagtaggc
     2581 tcattgcttt ttctccaaca aatgaaagta agtgttcatt tccaaccgat aaaagaataa
     2641 aatcattgat atggtacaaa agattgactt taaatcgtga atcaaaatcc aaattaaact
     2701 tgaatgattt aatagtgcaa ctagcgagga gtaacgacgc tatcggctat aatcaaaggt
     2761 ggaatatatt tgttgatgca ctgagtgttc taaatagtaa caacgattta gtaatcaaat
     2821 gtggcggtga aaattcacca tttgttaaat taaatgagct taattctcga ggcgaagaat
     2881 ctagactatc attatattct gaaattattc tctctggcga gcctttgagg cttattgaaa
     2941 ataaatgtta ccctttgagt agtggggaaa tatcgtttat taagttttgt gtacaagttt
     3001 gtttgtatat tgagaatggg actcttctat taatagatga gccagaaaca catctccatc
     3061 cttcatttat taataaattt atggcattgc ttgataattt attagaaaag acaggctctt
     3121 cagctatcat agcaacgcat tcggtttatc ttgttcggga ggtttttaag gagcaagtga
     3181 caatactcag aagacgagat gatggggtaa ttgttagcga aaaaccgaga ttgagtactt
     3241 ttggggcgaa tataggaaat atctcatatt ttgttttcgg tgagagtgaa ccaactcaaa
     3301 ttttaaataa agtaaaaaac aatataattg caaagaatat gtcttgggat gatgttgaat
     3361 ctaaatatag cgaggactta tcacttaatg tattaactgc gttgagaaat gaaatgggca
     3421 attgaaatga ataaattaaa aagacctgtt tttaaagatt attctgcatt taaaaaacta
     3481 tctgcaaata agagattaaa acatcattct gttctgaatg gacaggatac atttatactt
     3541 aatgcatata gatcgtacat aaagaataaa gggtatctta atgtcaattc ttgtggcgta
     3601 ttgagtaaca atattgctga agctttgaga tattattata acaatcccgc aaagtgttta
     3661 tctataattg atgatataag agcagctaat tctaactctc tatgtccaat gtgtggttca
     3721 atgcatagcg ggactttgga tcatgtttta cccaaggaaa actatccaga attttcttta
     3781 ttcactaaga atttggttcc tgcttgcaaa tgtaatactt tcaaaagtac tactatagct
     3841 aattctgcag gccatcggat gctgcatcca tattttgata atatattaaa acaaagatta
     3901 atatgtgcta atttttctaa attagggcga acacctacaa ttgatgtaaa aatcataatt
     3961 gacccaatgt caccagaata taacaatgtt aagtatcatc ttgataatgt tgttatcaag
     4021 aataatatta aggggtattt gtctgaacaa tgggagtcgt tttacttata tcctgagctt
     4081 gttattagag gactgaataa gagtctttct acttatagcg atgtatatga gttgttgact
     4141 cgcgagttgt tgctgttgga tgaaaaacat aaaggtaaaa ataattggaa ttcggttttt
     4201 gtagctgggt taattcgacc tgatgtgatt aggtggatac tctgtgaatt acatttaggt
     4261 aacagaagtc cagacggtcg tctcatatga ttttttgcat cttcagcgtg tatctgcacg
     4321 ctgaagaaat tcataaaaac tgctgcctat taataaattt ttttagctga gttaaagaac
     4381 ctcatttagt gattacgtta agtagctagt ttataactat caaatttaac aatgttctca
     4441 cttaccaatt tatttatttc catcattctt ttttgtaacg gtattaactc gttacgtaca
     4501 aacaccaggc tcgccttctc cacatcccca aaccccccga cattattagg cataatcccc
     4561 atcatttgcg gtggcacacg atgcgccgcc atcatgtcat cccggctcac gttcttgatg
     4621 ttcagaaact catccttcgc cgcgacttct gacaacggga tgatctgaag cccgtccttt
     4681 ttgccgttag gcgagtacat aaacagattg cggaagttac ctggtccttt ggcgcttttc
     4741 atcgcattgc ggaggttgtt cacatcctcc tggttttgcg cggcatcggt catgtacatg
     4801 atgaagcctg catgactgcc gttaatgtaa tactatcggc ggaacagcgt ggcggactcg
     4861 ttgagcaggg ctgacggaat ggcagaaagg taacctggca ggccgtagat ctcttggttg
     4921 atgtccggtt ccatcagatg aaagatgctt cctttcgtga actgatacgg ctgcgtggtc
     4981 atgccgtatt gcacaaacca gtaggtatcc aggtctaatc cgcgtcgggt gtattttgcc
     5041 agagcaggct caagagcgat gacctctccg aaccggtttg tgcgtttctc caggtaagcg
     5101 ttaccaaata ccagataatc ctgcacaaaa cgtgaaaaag cctgctggct gagcagcggg
     5161 tgagggatgt aggtactgat cagaatgttg catttcaccg caattggtga actgtgatgc
     5221 acggcggcgc ggaaggtgcg cgccagtccg tcaaaactca ctggcggctc ataccagcga
     5281 tctgtctgta cgcattccac atagtccagt agttcacggc ggtccagaac tggaacggga
     5341 tcgccgaagc tgaatgcttc ggctgaagtc tggtttttat gctgggtctg tttcgtcgcc
     5401 gcagcgcggt tcttcttact ctttcccatc aaaaaatctc cacaatattg ctggtattgg
     5461 cggactcgcc ctgcagcggt tcgttaaaca gtgcgtgcat tgttgcccag gccagatcgg
     5521 catggctggc ttcttcgctg cggctggctt cataggtcgg gcggttgccg ctggcggtgg
     5581 tggcgcgacg gattgccatg aatgactgcg caatgtcggt gtgcccggcg tcaaactcaa
     5641 gacggcggtg gctgataatg tcgtaggcct tgagtaccag ggcgttttta acgttggggt
     5701 tgtagacaaa ctcccggacg gcaggaaaaa acgctttcac gttctcgtaa accccgtgac
     5761 caacgccggt tgagtcgatg ccgatatagg tcacgttgta ctgttcagtc agttttttga
     5821 tggcgtaagc ctgggcgcga aagtccattc cgcgccactg gtgacgctca agaatgcgga
     5881 acttaccgcc tggcacggct ggcggtgcca ccaccacgca tccggcgctg tcgccgttct
     5941 gcgtaccttt tgccgggtca taaccgatcc acacttcgcg ccagccaaac gggcgcaggg
     6001 ccagtgcatg aaagtcggtc cagacttccc agctgtccac catgcacgcc tgcaattcgc
     6061 tgagcgggaa cacggacgcg agatcgtcca cgaactcgca catcagcagg ttctggtatt
     6121 cgtcagggct gtactccatg cgcaactggt caaggtcgaa caggttacag gcgccgcgca
     6181 ccgcatcttc cacggtgact atctggcggt attgcccgtc tgcgcacagc aggccggggg
     6241 ccagattgct gtgggacagg tcgatgtcca ccttgtcagc tttgttgcgc ccacggttga
     6301 acagcgcacc ggaccagaac ggataagcac tgtgggtcag actggatggt gtggaaaaat
     6361 aggtctgccg ccatttcttg tgaatagcca taccagaagc cactttgcgt agctcctgaa
     6421 atttcggtat ccagaaatat tcatccagat acaggttacc gtgataactc tgggcagtgc
     6481 gggcattggt gccgaggaaa tacagtgtgg ccccgttggg aagcaccatc ggatcgcctt
     6541 tcagctccac ctccacttct ttggcaaagt cgatgatgta ctgtttgaag acgtgggcct
     6601 gtgccttact ggcagaaagg aaaatctggt tacgtccggt aagcagggcg tcaatcaggg
     6661 cttcacgggc aaaatagaag gtcgcgccga tctggcgtga cttcagcagg ttgcggatgc
     6721 ggttggtttt tccggcttcc caccagtggc gctggtagtt gaacatggag gaatggaaat
     6781 ttttttccag cttctcaatc tggtcatcgg tgaaaacatt cttttcaggc tgacggcgtg
     6841 ggcctttgtt gcggttggcg acgttagggt ttaagtcggc ttcgttgccg ccattgttaa
     6901 acttgccgat ccgcgcgtgg cgctcagact ggcgcgccag caggtcaata tctttgaaat
     6961 ctttcccttc tttgtgctcc ttcatgatga gctggcagta gcgtgcggcg gtggtgagct
     7021 gcatctgatc cagaggccca tagtcacccc acttgtcgcg ttttttccag ctgtaaacgg
     7081 ttgcaacttt ctcgcccagc atttcagcaa tgcgggctac gcggtatccc tgaaagtaca
     7141 gcagcatggc ctgccgacgg ggatcgagat ctgcgggtgt cagtgtggtg ttcatggcac
     7201 aaacctacag ccttgaatga aggctttccc cgcctgcggt ttgtgtggtt gtcggtacaa
     7261 ataccgcgca ttgtttcact gcccctatca ccgcaaccat aaggctccag taagtttttt
     7321 ctaacggagc acggctcatg acagtgaaag caaagcgttt tcgcatcggg gtggaaggtg
     7381 ccaccaccga cggacgcgaa atccagcgtg aatggctgga acagatggca gccagctata
     7441 acccggcggt gtataccgcg ctgattaacc ttgagcacat caagtcttat ctgccggaca
     7501 gcacctttaa ccgctacggc aaggtgacgg cgctgtttgc tgaagaaatc acggaaggtc
     7561 cgctggcagg caagatggcg ctgtatgctg acgttgagcc aacggagtcc ctggtggaac
     7621 tggtgaaaaa aggccagaaa ttattcacct ctatggaagt cagcccgaag ttcgctgata
     7681 cgggcaaagc ctacctggtc ggcctggctg ccactgatga ccctgccagt ctgggcactg
     7741 aaatgctgac attcagcgcc agtgcagccc ataacccgct ggcaaaccgc aagcagaatc
     7801 ccgccaatct ctttaccgcc gcagaggaaa cggtgatcga actggaagaa gtccaggacg
     7861 acaaaccgtc cctgtttgcc cgcgtcacgg cgctgtttac caaaaaagag cagtccgatg
     7921 acgcccggtt ctctgatgtg cataaggccg tggagctggt cgccactgag cagcagaacc
     7981 tgagcgcacg caccgaaaaa tccctgtctg agcaggaaga acgcctgtct gagctggaga
     8041 ctgccctgca ggcacagcaa accgccttta acgaactggt ggacaagctg agtcatgaag
     8101 acagccgcca ggactaccgc cagcgtgcaa caggcggtaa cgcccccgct gacactctga
     8161 ccaattgctg atggagcaca aaacctgatg aagaagaata cccgctttgc ttttaacgct
     8221 tacctgcagc aactggcgcg tctgaacggt gtggcagttg aagaactgtc cagcaagttc
     8281 accgtagagc cgtccgtgca gcagacgctg gaagaccaga tccagcagtc cgccgctttc
     8341 ctgactctga ttaacgtcac gccagtgact gagcagtccg gtcagctgct ggggttggga
     8401 gttggcagca ccattgccgg aaccactgat accactgcga aagagcgtga acctgtcgat
     8461 ccgacgctga tggtcgatgt ggaatataaa tgcgagcaga ccaactttga cacggtgctg
     8521 acctacgcga agctggacct gtgggcgaag tttcaggatt ttcaggtgcg tatccgtgac
     8581 gccatcgtga aacgtcaggc actggaccgc atcatgatcg gctttaatgg cgtgaagcgt
     8641 gcgaaaacct ccaaccgtag cgaaaacccg ctgctgcagg atgtgaataa aggctggctg
     8701 cagaaaatcc gtgaggatgc accggatcac gtcatgggca gcaccaccac gggcggtgaa
     8761 accacaccgg gcgcagtgaa agtcgggaaa ggtggcgaat atgccaacct ggacgccgtg
     8821 gtgatggatg ccgtcaatga gcttatcgac gtggtctacc aggacgatga cgatctggtg
     8881 gtgatttgcg gtcgtgaact gctgtctgac aagtatttcc cgctggtcaa caaagagcag
     8941 gaaaacagtg aaaaactggc tgccgatatg atcatcagtc agaaacgcat gggtggcctg
     9001 caggccgtgc gtgcgccgtt cttcccgccg aatgcgctgc tgatcacccg tctggataac
     9061 ctgtccatct actggcagga agacacccgc cgccgttcag ttatcgacaa cccgaaacgt
     9121 gaccggattg aaaactttga atccgttaac gaagcctatg tggttgagga ctaccgctgc
     9181 gccgcactgg tggaaaacat ccagattagt gatttcagcg ccgccgcagc cgaaaccgga
     9241 gcgtaatcca tgagcctgag tcccgcacgg cagcatcgcc tgcgcgttca ggctgaacag
     9301 gccgcccgtg agggtggcag tgttcgccac gcatcgggct atgacctgat gctgctgcaa
     9361 ctggcggaag accgccgccg tctcaagggc gttcagtcca cggtcaaaaa agcggaaatc
     9421 aaagtggagc tgctgccgaa gtacgccgcc tgggcggagg gtgtcctggc tgccggaggc
     9481 gctcaacagg atgacgtgct gatgtacgtg atgttgtggc gcattgatgc cggagattat
     9541 gccggggcgc tggagatctg gcgtcatgcc ctgcgtcatg gatgggtgat gccgctgggc
     9601 aaccgcaacg tgcagaccgt gctgacagag gaaatggcag atgcagcgca gagcgcaatg
     9661 cttgccgcca ccggctttga tgccgatctg ttgctgcaga cgctggagct gacagacggt
     9721 ctggatatgc cggaccagtc acgggcacgt ctgcataaag cgattggcgc ggtcctgagt
     9781 gaaagcaatc cggcttccgc ccttaatcat ctcaaccatg cgttacagct cgatccccgc
     9841 tgtggcgtga aaaaagacaa acagcagctg gagcgcagac tgcgcaatga cagccgctga
     9901 cagaacgtgc ccccgcgcac gggcggcacg gggtggcgaa aggcacagcc acatcaaaac
     9961 cccgtccacc gccctttatt tcaggagaaa gcagcatgaa gtttgttgcg ccagaacagg
    10021 caccggaaca ggcggaaatc attagaaata cgccgttctg gcctgatgtg gacctgtcgg
    10081 agtttcgcag tgtcatgcgc actgacggca cggtgacgca gccgcgttta aagtaggttg
    10141 cactgtcggc aatttcggag gttaacgcag agctgtatga atttcgcaga cgccagcaga
    10201 tgctggggta tgcgtcggaa cagctggacg ggaaaagtga gcgcattcag cactatttca
    10261 acgcggttta ctgctgggct cgcgccatgc tcaacgaacg tttccaggac tatgacgcca
    10321 cggcatccgg tgcgaagcgg ggcgaagaac tggcggaagc aagcggtgat ttgtggcgtg
    10381 acgcccgctg ggccatcagc cgggtgcagg acgcgccgca ctgcacagtg gagcttatct
    10441 gatgaaagtg cgtgcgcatc agtatgacac ggtggacgcg ctttgctggc gtcattacgg
    10501 gcgcacgcag ggtgtcacgg agcaggtact gaaggcaaat ccggggcttg ccgaatacgg
    10561 cccctttgta cctcacgggc tgcaggtgga gctgccggac attccgacca ccaccaccgt
    10621 gcagaccgtc cagctatggg actgaattat gacgcttgag cgaatcagcg cctttatcac
    10681 gtattgcatc gccgtcgtgc tggcctggct gggcgatttg tccatcaagg atgcctcaac
    10741 gctgggcggc ctgatgattg gtgtgctgat gctggctatc aactggtact acaaacacaa
    10801 agcctaccag cttctgcgcg acgggcagat ctcgcgggag gactatgaat ccatcaatcg
    10861 ttaaacgctg ccttgtcggg gccgtgctgg ctattgctgc cacgctgccg ggttttcagc
    10921 agcttcacac ctccgtggag ggactgaaac tgattgccga ttacgaaggt tgtcgtctgc
    10981 agccgtatca gtgcagcgcg ggtgtctgga ccgacggtat tggtaatacg tcgggcgtca
    11041 ttcccggcaa aaccattacg gagcgacagg cagcagaagg gctgatctcc aacgtgctgc
    11101 gtgtggagcg ggcgcttgaa aggtgtgtga agcaacagcc gccgcagaag gtgtatgacg
    11161 ctgcggtgtc gtttgccttc aacgtgggta cgggcaatgc ctgcagttcc acgctggtga
    11221 aattgctcaa tcagcggcgc tgggcggatg cgtgccgaca gttgccgcgc tgggtttatg
    11281 tgaaaggtgt ttttaatcag gggctggata accgccgtgc gcgggagatg gcctggtgtt
    11341 tacagggagc aaactgaaat gaaaaagaaa ttaatcagcg ggctgtttct gatgttatgg
    11401 atggcgctgt taatcgcagc aatagtgtat ccgcagggga tttttccggt actggcagcg
    11461 tccggcgttt gggtagcctg tttactgaca tgggcggtaa ttccggtagc actggcttcg
    11521 ttaattaaga atggcccgct ctggcaggag ttgagggcat ctttactgaa gacaattacc
    11581 cgaaaagaaa acgtatttat cagctgggtg atacgattgc tgattgtcgt cagtctcgcc
    11641 tggacggggt gggccattac cctggtcttt tatctgctga ccgttattgc cttctggatc
    11701 acccgtaatc agatggcgca acaggtagcc gcatgaaccg gttgctgctg gttgtgctgg
    11761 cgttattatt ggcggcgctg ggctggcaga cgtggcggct ggctgatgcc agccagacca
    11821 tcagcacgca ggcagacgag ctgcagagca aaagccaggc actggcaaag agcaacagcc
    11881 agcttatcag cctgtccatt ctgactgaaa ccaataaccg ggagcaggcg cggctctatg
    11941 ccgaagcaga acagaccagt gtactgctga gacaacgaca acgccggatt gaggaactga
    12001 aacgtgagaa cgaggattta cgtcgctggg ctgatactcc tttgcctgct gacattatcc
    12061 ggctgcggga acgtccggca ctcaccggag gtgcagctta ccgtcagtgg ttgtccgcga
    12121 gtgacgccgt gtcggctgga gcaggcagcg ccgcgcacta acggtgatct gaatgcgttg
    12181 ctggatgaaa cggaggccgc ctgggcggtc tgtgcagaca aagtggacat gattattgcg
    12241 tgtcaggagc gaaatagtga acaaaccaca atccctgcgc cacgccctca ataaagcagt
    12301 gccttatgtc cgtaataacc cggacaaact gcatctgttt gtggataacg gttcgctggt
    12361 tgcctccggg gctagctcca tgtcatggga gtaccgctac accctgaacg tggtgattga
    12421 agatttcagc ggcgaccaga atctgctgat ggccccggtt ttgctgtggc tgcgggataa
    12481 ccagcccgat gcgatcaata acccggcgtt acgggaaaag ctattcacct ttgaggtgga
    12541 tattctgcgc aacgatgtct gtgatatcag ccttaacctg caactgacgg agcgtgtgct
    12601 ggtcagcact gacggcagtg tgtcgaacgt tgaagcggta gcggaacctg atgaacctga
    12661 agaaatgtgg acggtgaaac gtggctgaat tgcagaaggt ggacgactgg ctgagtgcat
    12721 tattggcgaa tctggagcca gccgcaagaa gccgcatgat gcgccagctg gcgcaggaac
    12781 tgcgccggac acagcagcag aatatcagga tgcagcgcaa tccagatggc agcagttatg
    12841 agccgcgcag ggtaacagca cgcagcaaaa aggggcgcat caaacgtcag atgtttacaa
    12901 agctgcgcac cacaaaatac ctgaaaactg cagccagcgc cgattctgcc agcgtacagt
    12961 ttgaaggtag ggtgcagcgc attgcccgta ttcaccatta cggcttgagt gatcgcgtca
    13021 gtcgaaaagg tatttatacc atctatgcga aaaggcagtt attaggtttt aatgtccggt
    13081 aatgctgcca acttactgat ttagtgtatg atggtgattt taaggtgctt gcgtggcttc
    13141 catttccatc aga
//
LOCUS       DAJTMK010000112        13099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTMK010000112 DAJTMK010000000
VERSION     DAJTMK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13099
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.113"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            7..1635
                     /locus_tag="OO135_002778"
     CDS             7..1635
                     /locus_tag="OO135_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR5398568.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTFEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1874..1933
                     /locus_tag="OO135_002779"
     CDS             1874..1933
                     /locus_tag="OO135_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5398569.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2250..2309
                     /gene="ibsE"
                     /locus_tag="OO135_002780"
     CDS             2250..2309
                     /gene="ibsE"
                     /locus_tag="OO135_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5398570.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2429..3862)
                     /gene="hldE"
                     /locus_tag="OO135_002781"
     CDS             complement(2429..3862)
                     /gene="hldE"
                     /locus_tag="OO135_002781"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5398571.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LELRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3910..6750)
                     /gene="glnE"
                     /locus_tag="OO135_002782"
     CDS             complement(3910..6750)
                     /gene="glnE"
                     /locus_tag="OO135_002782"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5398572.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQVDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVHREIMTLPREGKTLQTKVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6773..8074)
                     /gene="ygiF"
                     /locus_tag="OO135_002783"
     CDS             complement(6773..8074)
                     /gene="ygiF"
                     /locus_tag="OO135_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5398573.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8317..8937
                     /locus_tag="OO135_002784"
     CDS             8317..8937
                     /locus_tag="OO135_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5398574.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            9001..10239
                     /gene="cca"
                     /locus_tag="OO135_002785"
     CDS             9001..10239
                     /gene="cca"
                     /locus_tag="OO135_002785"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5398575.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREVL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10396..11217)
                     /gene="bacA"
                     /locus_tag="OO135_002786"
     CDS             complement(10396..11217)
                     /gene="bacA"
                     /locus_tag="OO135_002786"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5398576.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11308..11676)
                     /gene="folB"
                     /locus_tag="OO135_002787"
     CDS             complement(11308..11676)
                     /gene="folB"
                     /locus_tag="OO135_002787"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5398577.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11781..12398
                     /gene="plsY"
                     /locus_tag="OO135_002788"
     CDS             11781..12398
                     /gene="plsY"
                     /locus_tag="OO135_002788"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5398578.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12411..>13099)
                     /gene="ttdR"
                     /locus_tag="OO135_002789"
                     /gene_synonym="dan"
     CDS             complement(12411..>13099)
                     /gene="ttdR"
                     /locus_tag="OO135_002789"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR5398579.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIM
                     LRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
                     GKPDEGYQVM"
BASE COUNT         3162 a         3681 c         3283 g         2973 t
ORIGIN      
        1 tcctggatgt ttaccgcgat tattgccgta tgcattctgt ttattattgg aattattttc
       61 gccaggctct atcgtcgcgc ttcggcagag caagcttttg ttcgtactgg tttaggtggg
      121 caaaaagtgg tgatgagcgg tggcgcaatc gtgatgccga tctttcatga aataatcccc
      181 atcaatatga atactctgaa gctggaagtc agccgctcaa ccattgatag cctgattacg
      241 aaagatcgta tgcgtgtcga tgtagtagtc gctttctttg tacgggtaaa accgtctgta
      301 gaaggcatcg ccactgcggc gcagacgttg gggcaacgca cgctatcacc agaagactta
      361 cgtatgttgg ttgaagataa atttgtcgat gccctccgtg caacagctgc acaaatgacc
      421 atgcatgagt tacaggatac ccgcgagaac ttcgtgcagg gagtacaaaa tactgtcgca
      481 gaagacctgt cgaaaaacgg tcttgagctg gaaagcgttt cacttaccaa ctttaaccag
      541 acctcgaaag aacatttcaa tccgaacaat gcctttgacg ccgaaggttt aaccaaactg
      601 attcaggaga cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga tgtagaagtt
      661 gcggtgcgtg agaaaaaccg tgatgcactt tcgcgcaagt tggagattga acaacaagaa
      721 gcgtttatga cgtttgagca ggagcagcag gttaaaaccc gtactgccga acagaatgca
      781 cgtattgcgg cttttgaagc tgaacgtcgt cgtgaagcag agcagacgcg aattctggct
      841 gaacgacaaa ttcaggaaac ggagatcgag cgcgagcagg ccgtccgctc aagaaaggtt
      901 gaggctgaac gtgaagttcg tattaaagag atcgaacagc agcaggtcac cgaaatcgcc
      961 aaccagacga aatcgatcgc tattgccgcc aaatcggaac aacagtccca ggcagaagcg
     1021 cgtgctaacc ttgcactcgc agaagcagta agtgcgcaac aaaacgtaga aaccactcgc
     1081 cagaccgcag aagccgatcg tgctaaacaa gttgccctaa tcgctgccgc gcaggatgca
     1141 gaaaccaaag cggttgaact gaccgtgcga gcgaaagcag aaaaagaagc cgcagagatg
     1201 caggcggcgg ctatcgttga gttagccgaa gctacacgta aaaagggtct ggcggaagca
     1261 gaagcacaac gtgcgctgaa cgatgctatc aacgtacttt ctgatgaaca aaccagcctt
     1321 aaattcaaac tggccttgtt gcaggcgcta cctgcggtaa tagaaaaatc cgttgagccg
     1381 atgaaatcta ttgacggtat caagattatt caggtcgatg gtctgaatcg tggcagcgct
     1441 gcgggtgatg caaacacggg taatgtgggg ggcggaaacc tggcggaaca agcattatca
     1501 gccgctctct cttaccgcac acaggcaccg ctgattgact ccttgctcaa tgaaattggc
     1561 gtttcaggcg gctcactggc ggcattgact tcacccttaa cctcaacaac tcccgtcgcc
     1621 gaaaacgtag aataaatttt cagctatgaa cgtccctggc acaaaaagcc ggggattttt
     1681 tatatctgcg ttccgctaaa aactgcaaat gatcaggtcg ttgcaacgtt ttgcgtgacc
     1741 gctcggggaa ggcaaaactg cctctgggaa agcgttgcgc gaggtccggc gctcatcaac
     1801 aatcggggag tcagcaaagg gctgaaacgg gaaagcccct cccgaggaag gggccttaca
     1861 gaaggaaagg gttatgatga agctcgtcat catactgatt gtgttgttac tcgtaagttt
     1921 cgcagcttat taacagccaa tcagaggggg gagaaatcct ccctcaccct tgttccttta
     1981 ctttaggttg aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa
     2041 aaagccgggg attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc
     2101 agcgttttgc gtgactgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg
     2161 tccggcgctc atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg
     2221 aggaaggggc cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttgtgt
     2281 tgttactgtt aagtttcccg acttactaac aactgatcag aggggggaga aatcctccct
     2341 tacccttgtt cctttacttt aggttgaaaa aacaacagcg tcaataggcc tgcctgctac
     2401 gaagcgagat ctgtgaaccg ctttccggtt agcctttttt atcctgttgg atcttcttga
     2461 tgatgttagt ggtcgagcaa ccgtcttcga agttgagcac cagcacttcg ccgccgttgg
     2521 cccagacttc tttactcccg gcgatctctt ctggtttata gtcgccaccc ttcaccagta
     2581 aatctggcag gatcccggcg atcaagcgct gcggcgtgtc ttcttcaaac gacaccaccc
     2641 agtcgaccgc ttccagtgcg cccagcacaa tcatacgcag ttcgagtggg ttaacggggc
     2701 gggaatcccc tttcagccgt ttggtggagg catcgctgtt cacagcgaca atcagacggt
     2761 cacccagttt gcgagcattt gccagatagg agacgtgccc ggcgtgcagg atgtcaaaga
     2821 caccgttggt cattaccacc ttttcgccgc gtttacgcgc tgccgctacg gccagcttca
     2881 gttcctcttc ggtcatcacg ccaaagccgg tatctgcgcg cccacgcact gcattttcca
     2941 gctcgatcgg cgaaaccgtg gaggttccca gtttgccgac caccacgcca gccgccgcat
     3001 tggcaaagaa gcaggcttct tccagcgaat tacccgctgc cagcgttgcc gccaggacgc
     3061 caatcaccgt gtcgcccgca ccggtaacgt catacacttc ctgcgcctgg gttggcatat
     3121 gcagcggcgc tttacccggt tgcagcagcg acataccctg ttcggaacgg gtcactaaca
     3181 gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg
     3241 tcttacattt accgacaaca gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc
     3301 cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag caccggaacg cccgctttac
     3361 gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca
     3421 ccagcgcgcc aatcgaactc agcgcctgat taatccgctc gtgcagcggc tgcggatcaa
     3481 cgccttcgaa accttcttcg aaatccagac ggatcagctg ttggttgcgg gaaagtaccc
     3541 gtaatttggt gatggtcgga tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt
     3601 cggccagaga tttactcagc gcgcgcgctg catcgtcaat gcccgtcaac ccgaccaggc
     3661 gtgcattagc accgagagaa gcgatattca tcgccacgtt agccgcgccg cccggacgtt
     3721 cttcgatggt attcacttta accacgggca ccggcgcttc cggcgagata cggctggtgg
     3781 gaccgtacca gtaacgatcc agcattacat caccaaccac catcactcct gcacgttcaa
     3841 actctgacag cgttactttc attcctgtct cctgagagat tcaaaatttg cgcgcgataa
     3901 taccatactt cattcttcca ccagccactt ctgccagctt gcccgcacca gatcacgctc
     3961 tgcggtgaag caatcctccg acacatggcc cggcaattcc tgtaatgcca gatgatgaag
     4021 ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc gcttcctgct cttccataat
     4081 gtcgttttgc gccagcagct ccagaatacg cacgttgtct gaccagcgcg ttaacttcgg
     4141 tttttcatga gcgtagcgca acaccagata ttgggtaata aattcgatat cggtaattcc
     4201 gccttcatcg gctttgatat caaagcgatt acgatgttta ttgccgagat gagcgcgcat
     4261 tttctcgcgc atttcccgca ctttcgtttg cagagtttta ccttcacgcg gcagcgtcat
     4321 aatctcgcga tgcactgcgt caaagtgcgc ggtgagctgc ggatcgccgt acactacacg
     4381 cgcacgcacc agcgcctgat gctcccacgt ccaggcctcg tttttctgat aatcagcaaa
     4441 tgcttctgcg gatgtcacca gcattcccgc cgccccggac ggacgcagtc gtgcatccac
     4501 ttcatacaaa atgccggaag aggttcgcgt attgaacaga tgcataatgc gttgcgccag
     4561 acgcagataa aactgtcgcc cgtcgatttc ccgctcgccg tcagtcatcg catccattgg
     4621 gcaatcatgg aggaagatca ggtcaagatc ggaactgtag cctaactccc agccgcccag
     4681 cttgccgtag ccgaccaccg caaaaccacg gccttcgcga tcgttgaggt gatttggctt
     4741 accgtagcgg gccaccattt gaacccacgc ctgctgaacg acggcatcta tcatagcctc
     4801 tgccagccag gttaagtgat cgctcacttt catcaccggt agcgtaccag cgatatccgc
     4861 tgcggcgatg cgtaacagct gcgcctgttt gaactgacgc agcgcctcaa gctgttgctc
     4921 ttcgtcatct tccggcacgc gcagcaaata ctggcgcaac tcatcgcggt aggcatcggt
     4981 cgccgtcggc tggtaaagag tgtttggatc gagcaattca tccagcaata atggataacg
     5041 cgccagctgg ctggcaatca tcggcgatgc ggcacacaga gaaatcaaat gtttaagcgc
     5101 cgcagggaat tcactgagca attctaaata ggtggtgcgg gtaacaatcc ccaccagcaa
     5161 ggcggtaatg cgcgacagcg taacggcagc gtcttcacgc gcacagacat cacttagcag
     5221 atgtggcatc agatggtcga gcacctgacg tcctcgcggc ccgatagtac gcttatccag
     5281 ctctttgcgg aaatcagcaa tcagcgttag gacctgtttg cgatcatcct cgctaagatg
     5341 cgccagcact ggcgtagtgt catctacctg caacgcatcc tgccacagct cacgccactg
     5401 ttccgacagc gactcttcct gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac
     5461 ccggcgcaca ttggtcatat gtgcggtcag cgccccggtc agttgcggcc agtcagcaaa
     5521 gtccatcgcc cacgccagcc gcgcacggtt aagctcatca gaaggaagcg tttgggtttg
     5581 ttcgtcgtta atgctttgca gcaggttttc cagacgccgc aggaacagat atgccactcg
     5641 caattgttca gcatcgtttt cagaaagcag atgcagcgcg gcaatgacgc tgagcgttgg
     5701 cagtaaagag cgcgactgca gcgacggttc gcgcccaccg cgaatgagct ggaacacctg
     5761 aacgataaat tcaatttcgc gaatgccgcc tgcgccgagt ttgatattgt cggtcaaacc
     5821 acgtcgacgc acttcacggg caatcatccc tttcatgttg cgcagcgact gaatcacgct
     5881 gaaatcgatg taacgacgga aaacaaacgg gcgcagcatc gcacgcaact cgttagcata
     5941 gacgccttcg ctatcgccca taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg
     6001 cccctgctcc tggtaataat cctccaacgc ggcaaagctc agcaccagcg ggccactttc
     6061 gccaaacgga cgcagccgca tatccacgcg atagacgaag ccatcctgcg ttggttgatc
     6121 caacacttta atcagccgct gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg
     6181 gcgtccgccc tgcgtacaac catgttccgg ccaggcaaaa atcagatcga tatctgagga
     6241 gaaattcagt tccccaccgc ccagcttacc catgcctaaa atcagcagcg gttgcgcttc
     6301 gccctgcgca ttgcacggcg ttccccactc gcggcagcaa gcgtcataca gccagtcacg
     6361 cgcggcgaca atcagcgtct ccgccagatg gctgagctgc tgcaatatgc tctcttcagt
     6421 aaccaaagcc agcgtttgcg cccaggcgat gcgcaccata atgcgccgcc ggaatagccg
     6481 cagctcgcgc attaacccgg cttcgtcact cacattgctg agcgcctcct gcaaccatgc
     6541 cgcgtaatgc tgccattcat cagcctgcgg cgattggctt tccagttccg tcagccactc
     6601 tggatgcgca atcacgctgt cctgcacaaa atcactgaaa gtaagtactg acttcgcctg
     6661 tgcgctaagt gatttctcgg ctaaaggctc tggcagccgc tcaacaaccg tctgccagta
     6721 ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt atccttgcct gattaacgtt
     6781 ttccgctgtg caaccagaac ggttcctgat tgtttgcctc attacggaaa tgttcaattt
     6841 caatgcgttg cccggtcgca atagcgtgac gcagcccctg ccagttctcc agccaggctt
     6901 gcgcgacgac aggatcatag taacccgcca gcaacagtat tgagtcaata tcacgcgtca
     6961 gacgtggcaa ctggtcacgg tagcgatcgc ccaacggctg gcagaaaacg cttttcagtt
     7021 cagcggcatg gcgggaaaga tggatatcgg caaagcgttt gaaggaatcg ctcattttac
     7081 tctgcgcttt ggcatctaaa aacggctgcc aggctttgct taccagccat tcggtcaacg
     7141 ccagcttcgc cattgccgtt ttggtagagt agaccgccgt cacggcagaa accgcagaag
     7201 cgatggtcgc ttcgcattga gtcagcaaat cacgtaagtg agtgctcgct ttgcgcggca
     7261 caataccacc gaacagcatc agtgtatgac ggaccagacc aatggctgcc agcacctgtt
     7321 ctttcgccgc atcgttgccg cgtacccaca gttcttcatg atactgccat tgcgctaacg
     7381 ccagctcgag cgccgcttcc agcccctgtt ccacatcggc ttttgccgca acatgcaaaa
     7441 tggtggtcgg tttgatttca cgcgccggat tgccctgcgc cagatgatag ccacgcgccg
     7501 ctttgctcag gctgccctgg cgtaatccgg tttgcgatac cagttggttc gccagtttca
     7561 gcaccgcgcg cgtgtcgccg ctaagcagtt ccagttccag ctcacagata ggttcagcaa
     7621 attcccccgc tttcacctcc ccctgatcga gagcgatttc aattcggctg tcatcgaccg
     7681 ccaccagcca tttttcgcga taaaaatcgg tgctgaacag cggttgtacg cgggaagcga
     7741 gatcggcggg caattcgccg ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg
     7801 tcggttcgct caacgccacg ttatattccg ggcgctggtg taagccgcct gtcactcttc
     7861 ctgcaacttt catggtcatc tcatagcgac cgttttcgcc acgaatacgt aagcccatat
     7921 cgtgcccacg cagccagtta tccggcgttt cgtagtaaat attcagcaac tgcacggggt
     7981 catggtgctc gccgcccagc gtattgagat ggtcacgcaa cgcctcaacg gcactgtgat
     8041 taacaataaa ctttaattcg atttcctgag ccatggcctt tacttatcgg ttatgtcaca
     8101 taagtgacga tgaacggcga acttaatgcg atcttttttg tcagtagata gtattttgcg
     8161 ccaaattgcc atgcaacagg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac
     8221 ctgacacaga ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac
     8281 tatcgttcca ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga
     8341 ttaactttac tcgcacttag cgccactgcc gtctcacacg ctgaagaaac gcgctatgtt
     8401 tctgatgaac tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc
     8461 acggttaacg ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc
     8521 caggtgaaag acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag
     8581 ccaagcctgc gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa
     8641 ctcaccaata tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg
     8701 cagagcgaca gcgtgatcaa cggattaaaa gaagaaaatc agaaattgaa aaacgagctg
     8761 attgtcgcgc agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc
     8821 atcatcatgc aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt
     8881 ctggtactgc cacacctgat cccaacgcgc aaacgcaaag atcgctggat gaactaaatc
     8941 gccttcttcg ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc
     9001 gtgaagattt atctggttgg tggtgctgtt cgggatgcat tgttagggct accggtcaaa
     9061 gacagagatt gggtggtggt cggcagtacg ccacaggaga tgctcgacgc gggctaccag
     9121 caggtaggcc gcgattttcc tgtgtttctg catccgcaaa cgcatgaaga gtatgcgctg
     9181 gcacgtaccg aacggaaatc cggttccggt tacaccggtt ttacttgcta tgccgcaccg
     9241 gatgtcacgc tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag
     9301 gacgataacg gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg
     9361 ttgcgccatg tttcccccgc ttttggcgaa gatccgttac gcgttctgcg cgtggcgcgt
     9421 tttgctgcgc gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg
     9481 cgcgagatga cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag
     9541 acggaaagcg ccctcaccac ccgcaatccc caggtgttct tccaggtact gcgcgattgc
     9601 ggtgcacttc gcgttttatt cccggaaatt gacgccctgt ttggcgttcc ggcccctgcc
     9661 aggtggcatc cggaaatcga caccggtatt cataccttaa tgacgctctc aatggcggcg
     9721 atgctgagtc cgcaggttga tgtccgtttc gcaactttat gccacgatct cggtaaagga
     9781 ctgacgccgc cagaactttg gccgcgtcat catgggcatg gcccggcggg tgttaagtta
     9841 gtggaacaac tatgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg
     9901 gtggctgagt ttcacgatct catccacacc ttcccaatgc tgaacccaaa aaccatcgtc
     9961 aaattatttg attccatcga tgcctggcgt aaaccgcagc gtgtcgagca actggcgctg
    10021 accagcgagg ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc
    10081 cgttggttgc gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa
    10141 gcgggattta aaggtgtgga gattcgcgag gtgctgaccc gacggcggat tgcggcggta
    10201 gccagttgga aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca
    10261 cgaaccatcc cctcgcccct tcggggagag ggtgagggga acagaccagc accggtgcga
    10321 acaatcaccc ccaccccggc cctctcccta cagagcgagg gagaaaacca ggcctgctcc
    10381 cgaacctttt ctcaatcaaa agaacacgac gtacaccgca gccgccacaa taaagcgata
    10441 aatggcgaac gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat
    10501 cagcgccacc acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggttgt
    10561 caggaagccc cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggtaccgc
    10621 cagcaggaac gagaactcgg aagcagcgta acggctcacg cccatcagca tcccacctga
    10681 aatggtggcc ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat
    10741 cataaacgcc tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt
    10801 caggcattcg gcggcaatca gcaacaaacc gccaacgacc agtgcataca tcacatttat
    10861 cgggttaaac aatgacttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat
    10921 catccccagc aaaatgtgga tcagcgttaa acgacctttg ctttctcctt cgtgctgcaa
    10981 cgggcggcca aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac
    11041 cgccagaatt gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt cgccctcgaa
    11101 ccccaacaag tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt
    11161 caatccttcg accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa
    11221 taaaccccta aattattaaa atgtaccgct tgtccggact actgcagtaa gaccaggtta
    11281 taaccgtttg gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc
    11341 aatgattaca ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatgcg
    11401 cacccacggc gagttgaagc gtgctaacag cagctccgcc acctcttcag ccacgcgttc
    11461 caccagcgca aaacgcgccc cctcgacgtg gctgaccacc gtttctgcaa tgtcagcgta
    11521 actgaggcaa tccgccacat catcactttt cgccgcttta cggttatccc atgccatttc
    11581 gatatcgaac actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt
    11641 gattaccgaa agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta
    11701 accggatacc acttccggcg aaatgtgcgt attatccaca gattcatcgt tgaacacgaa
    11761 ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc
    11821 tctgcggctc catttccagt gccattctgg tttgccgctt gtgtgggctg cccgatccgc
    11881 gaaccagcgg ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag
    11941 cagccgtagc agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt
    12001 atgaattagg tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac
    12061 acatctggcc cgttttcttc ggatttaaag gaggaaaagg cgttgctacc gcttttggtg
    12121 ccatcgcacc cattggctgg gatctcaccg gagtaatggc gggaacctgg ttactgaccg
    12181 tgctattgag cggatactcg tcgctgggag cgattgtcag tgcactgatt gctccgtttt
    12241 atgtctggtg gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc
    12301 tgctgcgtca tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga
    12361 cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt tctggtggat ctacatgacc
    12421 tgatagcctt catcgggctt gcccagccgt tgctggcacc atgccgccag aaattccacg
    12481 cagacacgta atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg
    12541 ctttgtgcat actccggcaa tacttgcacc agtttgccac tctccagaaa cggcaacaca
    12601 tcccactcgg aacgcagcat aatccctttg ccctccagcg cccattgcaa cactatctcg
    12661 ccgctattgg aggaaagatg cccgctaacc ttcaccgatt ttttctcctg accgttcccc
    12721 aactcccata ttccgtgggt catatcgcgc tctttggtca ccagacagtc atgacgactt
    12781 aattcctgta atgattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat
    12841 attcttttat ttttcgttaa cagatgcgca atataataat caggaatttc gtcattaata
    12901 cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa caattcaaaa
    12961 tgcacctgta actcaggata attgcgcata agttcggtaa tggctggcgc aatatggctg
    13021 cgcccaaaac caaaactgca accaatacga atcatcccct ccgggcgcgt tttgatttgc
    13081 gtgacgtcat cgaccagtc
//
LOCUS       DAJTMK010000113        13098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTMK010000113 DAJTMK010000000
VERSION     DAJTMK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.114"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..723)
                     /locus_tag="OO135_002790"
                     /pseudo
     CDS             complement(1..723)
                     /locus_tag="OO135_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150766.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            1190..2422
                     /gene="mdtM"
                     /locus_tag="OO135_002791"
     CDS             1190..2422
                     /gene="mdtM"
                     /locus_tag="OO135_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR5398580.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGATTISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2463..3743
                     /locus_tag="OO135_002792"
     CDS             2463..3743
                     /locus_tag="OO135_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR5398581.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            3859..5012
                     /locus_tag="OO135_002793"
                     /pseudo
     CDS             3859..5012
                     /locus_tag="OO135_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5022..5789
                     /locus_tag="OO135_002794"
     CDS             5022..5789
                     /locus_tag="OO135_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398582.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            5786..6043
                     /locus_tag="OO135_002795"
     CDS             5786..6043
                     /locus_tag="OO135_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR5398583.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            5997..6968
                     /locus_tag="OO135_002796"
     CDS             5997..6968
                     /locus_tag="OO135_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR5398584.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNLIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7036..8214
                     /locus_tag="OO135_002797"
     CDS             7036..8214
                     /locus_tag="OO135_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398585.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGTFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(8227..8781)
                     /gene="kptA"
                     /locus_tag="OO135_002798"
     CDS             complement(8227..8781)
                     /gene="kptA"
                     /locus_tag="OO135_002798"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5398586.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9031..9714
                     /locus_tag="OO135_002799"
     CDS             9031..9714
                     /locus_tag="OO135_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398587.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9711..10172
                     /locus_tag="OO135_002800"
     CDS             9711..10172
                     /locus_tag="OO135_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5398588.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10185..11357
                     /gene="iadA"
                     /locus_tag="OO135_002801"
     CDS             10185..11357
                     /gene="iadA"
                     /locus_tag="OO135_002801"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5398589.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHIPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11422..12333
                     /gene="hypT"
                     /locus_tag="OO135_002802"
                     /gene_synonym="qseD"
     CDS             11422..12333
                     /gene="hypT"
                     /locus_tag="OO135_002802"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5398590.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12326..12718)
                     /gene="iraD"
                     /locus_tag="OO135_002803"
     CDS             complement(12326..12718)
                     /gene="iraD"
                     /locus_tag="OO135_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5398591.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12715..12798)
                     /gene="idlP"
                     /locus_tag="OO135_002804"
     CDS             complement(12715..12798)
                     /gene="idlP"
                     /locus_tag="OO135_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5398592.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         2952 a         3322 c         3361 g         3463 t
ORIGIN      
        1 ctaatgcctg catttgctct gccgaaagtc cggtatatcg ttgtatccat tcaggatcgg
       61 ccccattctg caacaacaat cggaggaggc tccgttcccc ttctactttc ccttctttcc
      121 tccccctctc ccttcccaac agccatccat cattatgaat tcgctctgca attgtcatta
      181 ttcgctccct gtggtgtggc attcgcctgg taagttcgct gataaacgcc ttaaaacgcg
      241 cttcatcgcc agtcagtaaa atgtaattta acagcgcagt tatctggctg tcattagcac
      301 actctgtaac cagtaatact accagttggt caataagccc catcaaatcg cgctggcgaa
      361 tatgcttttg gatcaactcc aacagggcga ctctgcgatg ctgcacaatc tcgtcgtctg
      421 gcacgacagt gacatccacc agcgggaacg ctgcgctata aagtttccgt gcggtagtcg
      481 gggcggcaaa ttcgtccagc cagcacaggg accaggggta aggactacgg ctaccgtgat
      541 aaaacagcat cgggataacc agcggtagcg gccggcgttt atcatgctcg atatggcgct
      601 gcatcaccgc catggaatat cgcatcaggc gaaatgccat atgaatatcc gcgcggtgtc
      661 agggtgagtg agaaaggatt taaataatgc gtcatgtggc gtgctggtcg tgaagtttgt
      721 catggcgatc cgtcacctgt agataagatg gcccgacttt agcgactaac aaaaccattc
      781 tcacccggca atttctcgtt ccccgaggtg ctttccaggc gtctgcaaac gatacatttt
      841 tccggaaaca ggctcgcgtt ttacatttcc tgtacccggc agatattccc ctttccaccg
      901 cgctcttttg attaaccgca aattttaatt acacttaagg tgtatatttt ctatgcaacc
      961 catcaattaa agaggtgtaa tgtgctgatg actatttgta atcgttatac attctgacct
     1021 ggagtcagaa ggtatttctc tgtctgtgtg ttcacaggca gtgtggttga ttacatgaaa
     1081 ttcagtacat ttgcagtctc gttgcccttc tcgcctgcct ttcgtcatta ccgacggtat
     1141 tgaatttcgt tttccccgtt ggggttctcc gaacaaggag ttgtttgtta tgccacgttt
     1201 ttttgcccgc catgccgcca cgctgttttt cccgatggcg ttgattttgt atgactttgc
     1261 cgcgtacctg tcgacggatc tgatccagcc ggggatcatt aatgtggtcc gtgattttaa
     1321 tgccgatgtc agtctggccc cggcagccgt cagtctctat ctcgcgggcg gtatggcgtt
     1381 acagtggctg ctggggccgc tttccgacag gattggccgc aggccggtgc tgattaccgg
     1441 ggcgctaata tttacccttg cctgcgctgc gacaatgttc acaacgtcta tgacacagtt
     1501 tcttatcgcg cgtgcaattc agggcaccag tatctgtttt attgccaccg ttggttatgt
     1561 cacggtgcag gaggcgttcg gccagacaaa agggatcaag ttgatggcga ttatcacctc
     1621 catcgtactg attgcgccga ttattggccc gctttccggc gcagctctga tgcactttgt
     1681 gcactggaaa gtcctttttg ccatcattgc agttatgggg tttatctcat ttgttggctt
     1741 actgctggcg atgccagaga cggtgaagcg cggcgcggtt ccatttagcg ccaaaagcgt
     1801 ctttcgcgat tttcgtaatg tcttttgcaa tcggctgttc ctctttggcg caacaaccat
     1861 ctctttaagc tatatcccaa tgatgagttg ggtggctgtc tcgccggtga tcctgatcga
     1921 cgcgggcagc ttaacaactt cgcagttcgc ctggacacag gttccggtgt tcggcgcggt
     1981 gattgtcgcg aatgccatcg tggcgcgttt cgttaaagat ccgaccgaac cgcgctttat
     2041 ctggcgtgct gtacccattc aactggtcgg cctcgcgctg ttgattatcg gcaatctgct
     2101 gtcaccgcac gtctggctgt ggtcggtgct gggcaccagt ttgtatgctt tcgggattgg
     2161 tttgatcttc ccgaccttat tccgctttac gcttttttcc aataacttac cgaaagggac
     2221 cgtctccgca tcgctgaata tggtgatcct gatggtgatg tctgtctcgg tcgaaatcgg
     2281 ccgctggctg tggtttaacg gcggtcgctt gccgtttcat ctgttagccg tcgtggcggg
     2341 cgttatcgtc gttttcaccc tggcaggatt gcttaatcgc gtgcgccaac atcaggctgc
     2401 cgagctggcg gaggagcggt gatttttgcg cgatcctgcc gtcaggctct attcttaacg
     2461 ttatgaataa actcattgaa ctcagacgcg ccaaaatgtt ggcgctctct ttactgctta
     2521 tcgccgctgc tacctttgtc gttacgctgt ttttgccgcc caatttttgg gtgagcggcg
     2581 tgaaggcgat tgctgaagcg gcgatggtcg gcgcgctggc ggactggttt gcggtggtgg
     2641 cgctgtttcg ccgcgtgccg attccgatca tttctcgtca tacggcgatt atcccgcgta
     2701 ataaagaccg gattggcgaa aatctcggtc agttcgtgca ggaaaaattt ctcgataccc
     2761 agtcgctggt ggcattgatt cgacgtcacg aaccggcgtt gttgattggc aactggttta
     2821 gtcagccgga gaacgcccgc cgcgttggtc agcatctgtt gcagatcatg agcggttttc
     2881 ttgaactgac cgatgatgcg cgtattcagc gcctgcttaa gcgcgcggtc catcgggcga
     2941 ttgataaggt cgatctttcc ggaaccagtg cgttgatgct ggagagtatg accaaaaacg
     3001 atcgtcatca ggtgctgctc gatacgctga tcgcacagtt gatcgccctt ctccagcgcg
     3061 ataaatcgcg caagtttatt gcccagcaaa ttgttcgctg gctggagagc gagcatccgc
     3121 tgaaagccaa aattttgcct actgaatggc tgggcgaaca tagcgcggag ttggtttctg
     3181 acgcggtgaa ttctttgctt gatgatatca gccgcgatcg tgcgcatcag atccgtcatg
     3241 cgtttgatcg cgccactttt gccctgatcg acaagctgaa aaacgatccg gaaatggcag
     3301 cgcgagccga tgccgtaaaa agttatctga aagaagatga agcttttaac cgctatctca
     3361 gtgaattgtg gggggattta cgggaatggc tgaaagcgga tatcaacagt gaagattctc
     3421 gtgtgaaaga acgtatcgcg cgggcgggtc aatggtttgg cgaaacgtta attgccgatg
     3481 atgccttgcg ggcatcgtta aatggtcacc tggaacaagc cgcgcaccgc gtcgcgcctg
     3541 agttttccgc attcctgacg cgccacatca gcgatacagt aaaaagctgg gatgctcgag
     3601 atatgtcgcg gcaaatcgag ttaaatatcg gcaaagatct gcagtttatc cgtgtcaacg
     3661 gtacgctggt tggcggttgt attgggctaa ttttatattt gttgtcgcag ctcccggcct
     3721 tgttccccct cagcaatttt tagaaatcca tgaaaaacta agtggctaac cgcgagagag
     3781 atcaaataaa ccatactaaa agaatggtaa taatgtggcc tcttttcatt atctcccgtg
     3841 gtacggggaa ggaaaatcat gtcacttatc accgatctac ccgccatttt cgatcagttc
     3901 tctgaagctc gccagaaagg ctttctcacc gtcatggatc tcaaggagcg cggcattccg
     3961 ctggttggca cttactgcac ctttatgccg caagagatcc cgatggcagc cggtgcggtt
     4021 gtggtttcgc tctgttctac ctctgataaa accattgaag aagcggagaa agatctgccg
     4081 cgcaacctct gcccgctgat taaaagcagc tacggcttcg gcaaaaccga taaatgcccc
     4141 tacttctact tttcggatct ggtggtcggt gaaaccacct gcgacggcaa aaagaaaatg
     4201 tatgaataca tggcggagtt taagcccgtt catgtgatgc agttgccgaa cagcgttaag
     4261 gacgatgcct cgcgtgcgtt atggaaagcc gagatgctgc gcttgcaaaa aacggtagaa
     4321 gaacgttttg ggcacgagat tagcgaagat gctctgcgcg atgccattgc gctgaaaaac
     4381 cgcgaacgtc gcgcactggc caatttttat catcttgggc agttaaatcc tccggcgctt
     4441 agcggcagcg acattctgaa agtggtctac ggcgcaacct tccggttcga taaagaggcg
     4501 ttgatcaatg aactggatgc aatgaccgcc cgcgttcgtc agcagtggga agaaggccag
     4561 cgactggacc cgcgtccacg cattttaatc accggctgcc cgattggcgg cgcagcagaa
     4621 aaagtggtgc gggcgattga agagaatggc ggctgggttg tcggttatga aaactgcact
     4681 ggggcgaaag cgaccgagct aatgcgtggc ggaaacgggc gatgtctacg acgcgctggc
     4741 ggataaatat ctggcgattg gctgctcctg tgtttcgccg aacgatcagc gcctgcaaat
     4801 gctcagccag atggtggaag aatatcaggt cgatggcgta gttgatgtga ttttgcaggc
     4861 gtgccatacc tacgcggtgg aatcgctggc gattaaacgt catgtgcgtc agcagcacaa
     4921 cattccttat atcgctattg aaacagacta ctccacctca gatgtcgggg cagctcagta
     4981 cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc agtgacatat tcgattggca
     5041 ttgattctgg ttctaccgcc accaaaggga tcttactagc agacggcgtg attacgcgcc
     5101 gtttcctcgt tccaacaccc tttcgtccgg caacagcaat tactgaagcc tgggaaactc
     5161 tgcgcgaagg gttagagaca acgccgtttc tgacgctcac cggctatgga cggcaactgg
     5221 tggattttgc cgataaacaa gtgacggaaa tctcctgtca cgggctgggc gcacggtttc
     5281 ttgcgccagc aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa gtgattcagc
     5341 ttgatgatga cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg gcgggcaccg
     5401 ggcgtttcct ggaagtgatc tcgcgcacgc ttggcaccag cgtcgagcaa ctcgacagca
     5461 ttaccgaaaa tgtcacgccg catgccatca cgagtatgtg cacagtgttt gccgaatcag
     5521 aagtgatcag cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc ggagtgatta
     5581 acgcgatggc gcggaggagt gccaatttca ttgctcgtct ctcctgtgaa gcgccgattc
     5641 tgtttactgg tggcgttagt cattgccaga cgtttgcccg gatgctggaa tctcacctgg
     5701 gaatgccggt aaatacccat cctgatgcgc aatttgctgg cgcaattggc gcggcggtaa
     5761 ttggtcaacg agtgaggaca cgccgatgaa agagttttta tttttatttc actccacagt
     5821 cggcgtcata caaacccgca aagcgttgca ggcagcgggc atgacttttc gcgtcagcga
     5881 tattccccgt gatttacgcg gcggctgcgg gttatgcatt tggctgacct gtccccccgg
     5941 cgaggaaata caatgggtga tccctgggct gactgagtca atttattgcc agcaggatgg
     6001 tgtctggcgc tgcattgcac attaccgggt ttctccgcgt taaggattca ttaaggtcgc
     6061 tacgtatact tctcgatatc ttattgattt gccttaaagg gattaccgtg acaaaaagta
     6121 tttcgttaag taaacgcatc tccgttattg tgattctttt tgccattgtg gcagtgtgta
     6181 ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc agcatcattt cagaattacc
     6241 acgccaccat tgacggcaaa gaaattgcag gcataacgaa taatatctct tcgctgacct
     6301 ggtcggcaca aagcaatact ctttttagca ctatcaacaa acctgccgcc attgttgaaa
     6361 tgaccaccaa tggtgatttt atccgcacaa ttcctctgga ttttgttaag gatctcgaga
     6421 cgatcgaata cattggcgat aatcagtttg tcattagcga tgagcgcgat tacgccattt
     6481 atgtgatttc actgacccca aactcggaag taaaaatcct caaaaaaata aaaatcccgc
     6541 tgcaagactc cccaaccaat tgcggttttg aagggttggc ttattctcgc caggatcaca
     6601 ctttctggtt ttttaaagag aaaaatctga ttgaggtcta caaagttaac gggctgttaa
     6661 gtagcaatga attacacatc agcaaagacg aggcactaca acggcaattt actttagatg
     6721 atgtgtccgg cgcagaattt aaccagcaaa aaaatacgct tctggtgctg tctcatgaat
     6781 ctcgcgcact ccaggaagtc accctggttg gcgaggttat cggtgaaata tcactaacca
     6841 aaggtagtcg aggtttgtcg cataacatca aacaggccga agggattgcg atggatgcct
     6901 ctggtaatat ttatatcgtt agcgaaccaa atcgttttta tcgctttacc cctaaatcat
     6961 cgcattaaca ggctggcata tttcaccttt ttgttattaa cttgttaaca acaaaaatga
     7021 catacaaggt taaacatgcc ttcgtcctcg catcccgtag aacgcttttc tttcagcacc
     7081 gcgcttttcg ggatgctggt tctgacccta ggtatgggat taggtcgctt cctttatacg
     7141 cctatgttgc ccgtcattat ggcggaaggg gcgttttcat ttagccagct ctcgtggatt
     7201 gccagcggca actatgcggg gtatctggct ggcagtctgc tattttcgtt tggcacattt
     7261 caccagccgt cgcgcctacg cccatttctg ttggtttctg ccctggcgag cgggttgttg
     7321 atcctcgcaa tggcatggtt gccgccattt attctggtgt tattgattcg cttcctggcg
     7381 ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc tgattatgca gcacacgcgc
     7441 catccttttg tgctggcagc tttgttttct ggcgttggca ttggcattgc actgggcaac
     7501 gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg gcaaggcgcc
     7561 ggagcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc gaaaaaacac
     7621 gctatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg gtggttactg
     7681 gctattctgt acggccttgc gggatttggt tatatcatcg tcgcgactta cctgccgctc
     7741 atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt agtcggctta
     7801 tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg ggctttgcct
     7861 tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct cgccagcgac
     7921 tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat gggcacgaca
     7981 tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa tcttttaggc
     8041 tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac cagtatgctc
     8101 ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc gttgtttatt
     8161 gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc atgaggagtg
     8221 tttcccttac cactcaagaa attcgaccgc aaccgttgat gtcagccaga ctccgttttc
     8281 cgcttgccag aaaggaatgc ctcgtttcgc catttcctgt gctttgacgg ttaagacaac
     8341 cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag agagatgcac
     8401 gtaatgacga tcccctgcaa tcagcccctg tttttttatc tcgtcaagga aacggcttgc
     8461 cgtaccgtgg tagagaaact gtggcggtgt tttttcggca aaggaaatag cgacctgcga
     8521 agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac taaaacgctt
     8581 tttatcgctg gttgcgacca cggtatccag caatgcacgg gttaatcgct tgcccgcttt
     8641 ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca gtacgatacc
     8701 aatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat ccgccagctc
     8761 tttttcgttg tgctttgcca tctcgttttc gtccctggat tttctgtttt tatttaccaa
     8821 tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgcaga accttttcca
     8881 cacagcgtaa tgtttcccct atgcaaaaaa cgcatagcca gaaacatact tgcattttcc
     8941 ccatacccac atggctgata aggtttttca gttccaggaa ttttcctggc tcaagcaaca
     9001 cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc tgtagccggg
     9061 gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct caccataatc
     9121 tttttttccg gtgttttttc cggaacagac agctggtggc gcgtttttga tttttccgtt
     9181 ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc atttcgcggc
     9241 gcgggtggtg cgggggctaa agatggcttt ctctttgcgc tggagctggc accgtcagtc
     9301 attctctctc tggggattat ttcaattacc gatggtcttg gtggattacg cgccgcgcag
     9361 cagttaatga cacctgtgct aaaaccgttg ttgggcattc cagggatctg ctcgctggcg
     9421 ctgatcgcta acttgcaaaa cactgatgcc gccgccggta tgaccaaaga gctggcgcag
     9481 gaaggtgaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac cagcggtagc
     9541 gccatcatca ccaactactt ctcttccggc gtcgcggtgt tcgcctttct gggcacgtcc
     9601 gtaatcgtac ctttagccgt catcctggtg tttaaatttg tcggagccaa cattttgcgc
     9661 gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc atgacaactc
     9721 aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc tttactatcg
     9781 ccacgaccaa cctgctgcca aacgtggtga tggcgtttgt catcattcag gcgctgaaaa
     9841 ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg ctgtggggat
     9901 tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggt ggcgcagtcg
     9961 gtgttgccgc cagtctggca accgctggcg cattaaccgg tcacgatgtc accgttttac
    10021 tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc ctggggacag
    10081 ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt aacgcattgc
    10141 tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt gattataccg
    10201 cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat cggggaattt
    10261 gcgatgtcct cgtcgctaac ggcaaaatta tcgccgttgc cagcaatatc ccttctgaca
    10321 ttgtaccgaa ctgcacggtt gtcgatctca gtgggcagat cctctgccca ggttttattg
    10381 atcaacacgt ccatttgatt ggcggtggcg gcgaagcagg tcccacgacg cgcacgccgg
    10441 aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt ctgctgggca
    10501 ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg ctcaatgaag
    10561 aaggcatcag cgcctggatg ctgaccggcg cttatcatat cccttcccgc actattacgg
    10621 gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa tgcgccatct
    10681 ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg gcagaatccc
    10741 gcgttggcgg tttgctcggc agtaaacctg gcgtcaccgt gttccacatg ggcgacagta
    10801 aaaaggcgtt acagcctatt tatgacctgc tggaaaactg cgatgtgccg atcagcaagc
    10861 tgctgccgac ccacgttaac cgcaacgtac cgttgtttga gcaggcgctg gagttcgcgc
    10921 gcaaaggcgg caccatcgat atcaccagca gcattgacga accggtcgcc cctgccgaag
    10981 gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc agctccgacg
    11041 gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc ggtgttgcag
    11101 gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agactatgat ttcagtatca
    11161 gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg agcgggaaag
    11221 gcgaaattct gcctggcaat gacgcagact tactagtcat gacgccagaa ttgcgcattg
    11281 agcaggtata tgctcgcggc aaactgatgg tcaaagacgg caaagcctgc gtgaaaggaa
    11341 cgtttgaaac ggcttaaacg gttgtcagcc gccctaatgg acgtagaatg cccgcaaggg
    11401 cggctgacag agtaaaacgt aatggatgac tgtggtgcgg ttttgcataa tattgaaacc
    11461 aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca ggcggcagtc
    11521 agtcgtaacg tctcgcaacc ggcattcagc cgccgcatcc gcgcgctgga acaggcgatt
    11581 ggcgttgaat tgtttaaccg ccaggtgacg ccgctgcaac tctcagaaca agggaaaatc
    11641 ttccattcgc agatccgcca tctgttgcaa cagttagaaa gtaaccttgc agagctgcgt
    11701 ggcggcagcg attacgcgca acgtaaaatc aagatagccg ctgcacactc tctttccctc
    11761 ggtctgttac cgtccattat cagccagatg ccgccgctct ttacctgggc gattgaagct
    11821 attgatgtcg atgaagcagt cgataaactg cgtgaagggc aaagtgactg tattttttcc
    11881 tttcacgacg aagatttgct ggaagcaccg tttgaccaca ttcgcttatt tgaatctcaa
    11941 ttgttccccg tttgcgccag tgacgaatac ggagaagcaa tttttaacct cgcgcagcca
    12001 cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat taatcgcacc
    12061 ctgacgcgcc acagcgagtt aagtttcagc accttttttg tctcttcgat gagcgagcta
    12121 ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta tgccatccaa
    12181 caagaaattc gcagcgggca actcgttgtg ctgaatcggg acgaactggt gatcccgatt
    12241 caggcctacg cataccgtat gaatacccgc atgaatccag ttgccgaacg cttctggcgt
    12301 gaactgcgcg agctggagat tgtgcttagc tgacattctc aagcgtcgca ctgcgccaac
    12361 cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac agcattatct
    12421 caaggctgat cgccagagtg tggcagtacg cttctttcaa attaacctgc aacgccgcaa
    12481 tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag ctctggagat
    12541 gtttgtcgct cgggttatgc acttgccact cgggcatgcc tgaaacaagt aatgacggta
    12601 atgccgaatg aggagaatta aaaagagtga ggagatcgct gcataccgat ttacgcagca
    12661 atgacgtttt attgcctgaa atttcaggta aaacagcctg aagtgattgg cgcatcattt
    12721 tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg caccactgta
    12781 ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc agtctgagac
    12841 aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaatat ctactgactt
    12901 ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa ccgctagtat
    12961 attctgaata ttccgaatag ttacgtagat gaaaatactt gtcaccgccg ctacttaatt
    13021 ccggtgggaa attatttatt tccgcataat cagccatgta ttatttgaaa caattagttt
    13081 gtaaataata taaattga
//
LOCUS       DAJTMK010000114        12898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTMK010000114 DAJTMK010000000
VERSION     DAJTMK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.115"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            41..910
                     /locus_tag="OO135_002805"
     CDS             41..910
                     /locus_tag="OO135_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5398593.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1113..1466)
                     /locus_tag="OO135_002806"
     CDS             complement(1113..1466)
                     /locus_tag="OO135_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5398594.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1604..3250)
                     /gene="groL"
                     /locus_tag="OO135_002807"
     CDS             complement(1604..3250)
                     /gene="groL"
                     /locus_tag="OO135_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5398595.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3294..3587)
                     /locus_tag="OO135_002808"
     CDS             complement(3294..3587)
                     /locus_tag="OO135_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5398596.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3863..5119
                     /gene="yjeH"
                     /locus_tag="OO135_002809"
     CDS             3863..5119
                     /gene="yjeH"
                     /locus_tag="OO135_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5398597.1"
                     /translation="MSGLKQELRLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPALGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCSVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGITT"
     gene            complement(5135..5611)
                     /gene="fxsA"
                     /locus_tag="OO135_002810"
     CDS             complement(5135..5611)
                     /gene="fxsA"
                     /locus_tag="OO135_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5398598.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5948..7384
                     /gene="aspA"
                     /locus_tag="OO135_002811"
     CDS             5948..7384
                     /gene="aspA"
                     /locus_tag="OO135_002811"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5398599.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7502..8803
                     /gene="dcuA"
                     /locus_tag="OO135_002812"
     CDS             7502..8803
                     /gene="dcuA"
                     /locus_tag="OO135_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5398600.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8919..9257
                     /gene="cutA"
                     /locus_tag="OO135_002813"
     CDS             8919..9257
                     /gene="cutA"
                     /locus_tag="OO135_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5398601.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKYHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9233..10930
                     /gene="dsbD"
                     /locus_tag="OO135_002814"
     CDS             9233..10930
                     /gene="dsbD"
                     /locus_tag="OO135_002814"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5398602.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLTIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10967..11542
                     /locus_tag="OO135_002815"
     CDS             10967..11542
                     /locus_tag="OO135_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5398603.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11649..11724
                     /locus_tag="OO135_002816"
     tRNA            11649..11724
                     /locus_tag="OO135_002816"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11682..11684,aa:Phe,seq:gaa)
     gene            11922..>12898
                     /locus_tag="OO135_002817"
     CDS             11922..>12898
                     /locus_tag="OO135_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5398604.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRATEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         3126 a         3352 c         3158 g         3262 t
ORIGIN      
        1 actaaatcag taagttggca gcattaccgg gagaacctac atggccataa gcatcaaggg
       61 tgttaatacc ggtgttattc gcaagagtaa taatttcatt gcactggcgc tgaaaattaa
      121 agaacctcgc aataaagaat cgttgttctt tatgtctgtc atggaactgc gcgacctgct
      181 gattgcgctg gaaagtcgtc tgcatcaaaa gcataaactg gatgctgctg cacgccttca
      241 gtatgagcaa gctcgcgata aagtcattaa gaaaatggca gaaaacatcc cagaaattct
      301 ggttgatgag cttaaaaatg ccgatattaa tcgtcgggtt aacacgctgg aacttaccga
      361 taatcaaggc gaaaatctta ccttcgtatt aacgctacat gacggtagca aatgtgagtt
      421 agtcgtcaat gagttgcaga ttgaaatgct agcgcgagca atcattcatg caattaataa
      481 tgcggaaatg cgtgaactgg cattacgtat tacctcactg ttagatttcc tgccgttata
      541 tgacgttgac tgccaggaaa atggcaatct ggaatatgac acctattccc aaccggaatg
      601 gaaacataac ttgttcgacc attatctggc ggtgctttac cgctttaaag atgaaagtgg
      661 caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa gcaacgccgg gtaaagaagt
      721 agaggcgatc actcgccgaa tgcttgattt cagcccacga ctgaaaaaac tcgcgggtgt
      781 gccatgtcag gtctatgttc gcaccgtagc ggcgaataac gcccaaccgc taacccagga
      841 tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc accagcaaaa ctgcaccaca
      901 ggcgaaataa tctcatcttt gctaatgcga ggctggagcc atccagcctg aaatgactga
      961 taacgtgatc gttggtcatg ccggatgcgg cgtaaacgcc ttatccggcc tacaaattct
     1021 caagcattca atcagttgca atgcctgctg taggcctgat aagcgaagcg catcaggcaa
     1081 taatacgttt gtcacccagg ctctggatca catcagttcg ggcacttata aacctgaccg
     1141 ataatctggc tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg
     1201 ccataaatca cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg
     1261 cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc
     1321 gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga
     1381 ccggcagcac tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca
     1441 atccctgcta agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact
     1501 cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca
     1561 tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca
     1621 tgccacccat gccgcccata ccgccagcag cgcctaagtc agctgcatcg tttttcggca
     1681 ggtcggtaac catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca
     1741 gagcagaacg ggttactttg gttggatcca ggatacccat gtcgatcatg ttgccgtatt
     1801 cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacagtg ttagcaacaa
     1861 cagacggttc ttcgccgcag ttcaatacga tctgacgcag cggagcttcc attgcacgca
     1921 gtgcaacttt gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag
     1981 acgctacgcg gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac
     2041 gggtcgcgtg cagggcatct tcaacgcgtg cttttttctc tttcatttca acttcggtag
     2101 cagcacctac tttgataact gcaacgccgc ctgccagttt cgctacgcgc tcctgcagtt
     2161 tttcacggtc gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc
     2221 cctggattgc agcttcttca cccacgccat caatgatggt ggtggtgtct ttgttgatca
     2281 caacacgttt agcctgaccc aggtcttcca gggttgcttt ttccagctcc ataccgatct
     2341 cttcagagat tacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacggc
     2401 gatcgccgaa gcccggagct ttaaccgcag caactttcac gatgccacgc atggtgttaa
     2461 caaccagagt tgccagcgct tcgccttcaa catcttcagc gatgatcagc agcggtttac
     2521 ctgctttagc aacggcttcc agaaccggca gcatttcgcg gatgttggag attttcttgt
     2581 ccgccagcag gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga
     2641 agtaaggaga caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct
     2701 gcagaccggt accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt
     2761 cagcgatcag tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa
     2821 tcgctttaga gtcagagcat ggtacggaca gcgctttcag ttcttcaact gcagcggtaa
     2881 cagctttgtc gataccacgt ttcaggtcca tcgggttcat gcccgcagca actgctttca
     2941 gaccctcagt gatgatagcc tgagccagta cggttgcagt ggtggtaccg tcgcctgcag
     3001 cgtcgttcgc tttagaggca acttctttca ccatctgcgc acccatattt tcgaacttgt
     3061 cttccagttc gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga
     3121 aagatttatc cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg
     3181 ccagtacgtt tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt
     3241 tagctgccat tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct
     3301 tcaacaattg ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc
     3361 tcagatttca caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc
     3421 ggcttcactt cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg
     3481 gtggatttag ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact
     3541 tctttacgct tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct
     3601 ttgagaaagt ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg
     3661 accagagaaa tggggatgag gattcgcccc ttcaaggggg aaaaagaaaa aaaattctga
     3721 tcagcacaaa atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc
     3781 gccgtcataa gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt
     3841 gggcgcaatc ggatatcaac ccatgagtgg actcaaacaa gaactgaggc tggcccaggg
     3901 cattggcctg ctatcgacgt cattattagg caccggcgtg tttgccgttc ctgcgttagc
     3961 tgcacaggta gcaggcaata acagcctgtg ggcgtggccc gttttgatta tcttagtgtt
     4021 cccgattgcg attgtgtttg cgattctggg tcgccactat cccagcgcag gcggtgtcgc
     4081 gcacttcgtc ggtatggcgt ttggttcgcg gcttgagcga gtcaccggct ggttgttttt
     4141 atcggtcatt cccgtgggtt tgccagccgc gctacaaatt gccgccgggt tcggccaggc
     4201 aatgtttggc tggcatagct ggcaactgtt gttggcagaa ctcggtacgc tggcgctggt
     4261 gtggtatatc ggtactcgcg gtgccagttc cagtgctaat ctacaaaccg ttattgccgg
     4321 acttatcgtc gcgctgattg tcgctatctg gtgggcgggc gatatcaaac ctgcgaatat
     4381 ccccttcccg gcacttggta atatcgaact taccgggtta tttgctgcgt tatcagtgat
     4441 gttctggtgt tttgtcggtc tggaagcatt tgcccatctt gcctcggaat ttaaaaatcc
     4501 agagcgtgat tttcctcgcg cattgatgat tggtctgctg ctggcaggat tagtctactg
     4561 gggctgtacg gtagtcgtct tacacttcga cgcctatggt gaaaaaatgg cggcggcagc
     4621 atcgcttcca aaaattgtag tgcaactgtt cggtgtagga gcgttatgga ttgcctgcgt
     4681 gattggctat ctggcctgct ttgccagtct caacatttat atacagagct tcgcccgcct
     4741 ggtctggtcg caggcgcaac ataatcctga ccactacctg gcacgtctct cttctcgcca
     4801 tatcccgaat aatgccctca atgcggtgct cggctgcagt gtggtgagca ctttggtgat
     4861 tcatgcttta gagatcaatc tggacgctct tattatttat gccaatggca tctttattat
     4921 gatttatctg ttatgcatgc tggcaggctg taaattattg caaggacgtt atcgactact
     4981 ggcggtggtt ggcgggctgt tatgcgttct gttactggca atggtcggct ggaaaagtct
     5041 ctatgcgctg atcgtgctgg cggggttatg gctgttgctg ccaaaacgaa aaacgccgga
     5101 aaatggcata accacataat ccggcgtttc gacattaatc ctggcgatcg tctttatgat
     5161 caaggcggtc gcgctcatca tcctttcgct ggtactcacc atcaaaagta ttaccgccac
     5221 cggtcccggc gctaaaaccg ccgcctggca tacgagaaaa gcgcaaatgc ggcatcaact
     5281 tcactgtcag atgcttttgc accggcggta ataaaagcag aagaccgagg aagtcggtga
     5341 aaaagcccgg taataaaagc agcaaaccgg caatgatcag cgaaacactt ttaatcatct
     5401 ccgccgctgg gttttcaccc gccgccattt tttgctgcat cagcacgaaa ttcttaaagc
     5461 cctggttacg taccagtgac atcccgataa ctgacgtgaa tataaccagc acgagggtca
     5521 gcaatacccc caatacatgg gcaacctgaa taaagattga aatctcaata tagacataaa
     5581 ggaaaatggc aataaaaggt aaccagcgca aaggtttctc ctgtaatagc agccggtaaa
     5641 acccggctac ctgaatgggt tgcgaatcgc gttcagctta tattgtggtc attagcaaaa
     5701 tttcaagatg tttgcgcaac tatttttggt agtaatccca aagcggtgat ctatttcaca
     5761 aattaataat taaggggtaa aaaacgacac ttaaagtgat ccagattacg gtagaaatcc
     5821 tcaagcagca tatgatctcg ggtattcggt cgatgcaggg gataatcgtc ggtcgaaaaa
     5881 cattcgaaac cacatatatt ctgtgtgtat aaagcaaatc attggcagct tgaaaaagaa
     5941 ggttcacatg tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc
     6001 agctgatgcc tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa
     6061 caacaaaatc agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc
     6121 agctatggca aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc
     6181 cgcatgtgat gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta
     6241 ccagggcggc gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg
     6301 tctggaactg atgggtcacc agaaaggtga atatcagtac ctgaacccga acgaccatgt
     6361 taacaaatgt cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc
     6421 ttccctgatt aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc
     6481 tgtcgaattc caggacatcc tgaaaatggg tcgtacccag ctgcaggacg cagtaccgat
     6541 gaccctcggt caggaattcc gcgctttcag catcctgctg aaagaagaag tgaaaaatat
     6601 ccaacgtacc gctgaactgc tgctggaagt taaccttggc gcaacagcaa tcggtactgg
     6661 tctgaacacg ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagtcactgg
     6721 cttcccatgc gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt
     6781 tatggttcac ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct
     6841 gcgcttgctc tcttctggcc cacgtgccgg cctgaacgag atcaacctgc cggaactgca
     6901 ggcgggctct tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca
     6961 ggtatgcttc aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca
     7021 gctgcagttg aacgtaatgg agccggtcat tggccaggcc atgttcgaat ccgttcacat
     7081 tctgaccaac gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa
     7141 agaagtgtgc gaaggttacg tttacaactc tatcggtatc gttacttacc tgaacccgtt
     7201 catcggtcac cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt
     7261 acgtgaagtc gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc
     7321 cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca
     7381 gtaatcgtac agggtagtac aaataaagaa ggcacgtcag atgacgtgcc ttttttcttg
     7441 tgagcagtaa cttaaaaaca acaatctaat atcaacttgt taaaaaacaa ggaaggctaa
     7501 tatgctagtt gttgaactca tcatagtttt gctggcgatc ttcttgggcg ccagattggg
     7561 gggaataggt attggttttg caggcggatt gggggtgctg gttcttgccg ctattggcgt
     7621 taaacccggt aacatcccgt tcgatgtcat ttccattatc atggcggtta tcgccgctat
     7681 ttctgccatg caggttgctg gcggtctgga ctatctggtt catcagacag aaaagctgct
     7741 gcgccgtaac ccgaaataca tcacgatcct cgcacctatc gtgacctatt tcctgacaat
     7801 ctttgctggt actggcaaca tctctctggc gacactgcca gttatcgctg aagttgcgaa
     7861 ggaacaaggc gttaaacctt gccgtccgct ctctactgca gtggtatccg cgcagattgc
     7921 gatcaccgca tcgccaatct cagcggcagt ggtttacatg tcttccgtga tggaaggtca
     7981 tggcatcagc tacctccatc tgctctccgt ggtcatcccg tccaccctgc tggcggttct
     8041 ggtgatgtcc tttctggtca ctatgctgtt caactccaaa ctctctgacg atccgattta
     8101 tcgcaagcgt ctggaagagg gcctggttga actgcgcggt gaaaagcaga ttgaaatcaa
     8161 atccggtgca aaaacgtccg tctggctgtt cctgctgggc gtagttggcg tggttatcta
     8221 tgcaatcatc aacagcccaa gcatgggtct ggttgaaaaa ccgctgatga acaccaccaa
     8281 cgcaatcctg atcatcatgc tcagcgttgc aactctgacc accgttatct gtaaagtcga
     8341 taccgacaac atcctcaact ccagcacctt caaagcaggt atgagcgcct gtatttgtat
     8401 cctgggtgtt gcgtggctgg gcgatacttt cgtttccaac aacatcgact ggatcaaaga
     8461 taccgctggt gaagtgattc agggtcatcc gtggctgctg gccgtcatct tcttctttgc
     8521 ttctgctctg ctgtactctc aggctgcaac cgcaaaagca ctgatgccga tggctctggc
     8581 actgaacgtt tcaccgctga ccgctgttgc ttctttcgct gcggtgtctg gtctgttcat
     8641 tctgccgacc tacccgacgc tggttgctgc ggtacagatg gatgacacgg gtactacccg
     8701 tatcggtaaa ttcgtcttca accatccgtt cttcatcccg ggtactctgg gtgttgccct
     8761 ggccgtttgc ttcggcttcg tgctgggtag cttcatgctg taatgatcaa tcgcggggcg
     8821 ttcacgcccc gctttctttc ccgccgacta acatccttcc cccgtacgtt gtatagtgac
     8881 ctctctcttg cggttccatc tgttcttgcg aggtgtttat gcttgatgaa aaaagttcga
     8941 ataccgcgtc tgtcgtggtg ctatgtacgg caccagatga agcgacagcc caggatttag
     9001 ctgccaaagt gctggcggaa aaactggcgg cctgcgcgac cttgatcccc ggcgctacct
     9061 ctctctatta ctgggaaggt aagctggagc aagaatacga agtgcagatg attttaaaaa
     9121 ctaccgtatc tcaccagcag gcactgctgg aatgcctgaa gtatcatcat ccatatcaaa
     9181 ccccggaact tctggtttta cctgttacac acggagacac agattacctc tcatggctca
     9241 acgcatcttt acgctgatcc tgctactttg cagcacttcc gtttttgccg gattattcga
     9301 cgcgccggga cgttcacaat ttgtccccgc ggatcaagcc tttacttttg attttcagca
     9361 aaaccaacat gaccttaatc tgacctggca gatcaaagac ggttactacc tctaccgtaa
     9421 acagatccgc attacgccgg aacacgcgaa aattgccgac gtgcagctgc cgcaaggcgt
     9481 ctggcatgaa gatgagtttt acggcaaaag cgagatttac cgcgatcggc tgacgcttcc
     9541 cgtaaccatc aaccaggcga gtgcgggagc gacgttaact gtcacctacc agggctgtgc
     9601 tgatgccggt ttctgttatc cgccagaaac caaaaccgtt ccgttaagcg aagtggtcgc
     9661 caacaacgca gcgtcacagc ctgtgtctgt ttcgcagcaa gagcagcaca ccgcgcaatt
     9721 gcccttttcc gcgctctggg cgttgttgat cggtattggt atcgccttta cgccatgcgt
     9781 gctgccaatg tacccactga tttctggcat cgtgctgggt ggtaaacagc ggctctccac
     9841 tgccagagca ttgttgctga cctttattta tgtgcagggg atggcactga cttacacggc
     9901 gctgggtctg gtagttgccg ccgcagggtt acagttccag gcggcgctac agcacccata
     9961 cgtgctcatt ggcctcacca tcgtctttac cttgctggcg atgtcaatgt ttggcttgtt
    10021 tactctacaa ctcccctctt cgctgcaaac acgtctcacg ttgatgagca atcgccaaca
    10081 gggcggctca cctggcggtg tgtttgttat gggggcgatt gccggactta tctgttcacc
    10141 atgcaccacc gcaccgctta gcgcgattct gctgtatatc gcccaaagcg ggaacatgtg
    10201 gctgggcggc ggcacgcttt atctctatgc gttgggcatg ggcctgccgc tgatgctaat
    10261 taccgtcttt ggtaaccgct tgctgccgaa aagcggcccg tggatggaac aagtcaaaac
    10321 cgcgtttggt tttgtgatcc tcgcactgcc ggtcttcctg ctggagcgag tgattggtga
    10381 tgtatgggga ttacgcttgt ggtcggcgct gggtgtcgca ttctttggct gggcctttat
    10441 caccagccta caggctaaac gcggctggat gcgcgtggtg caaataatcc tgctggcagc
    10501 ggcattggtt agcgtgcgcc cacttcagga ttgggcattt ggtgcgacgc ataccgcgca
    10561 aactcagacg catctcaact ttacacaaat caaaacggta gatgagttaa atcaggcgct
    10621 cgttgaagcc aaaggcaaac cggtgatgtt agatctttat gccgactggt gcgtcgcctg
    10681 taaagagttt gagaaataca ccttcagcga cccgcaggtg caaaaagcgt tagcagacac
    10741 ggtcttactt caggccaaca tcacggccaa cgacgcacaa gatgtggcgc tgttaaagca
    10801 tcttaatgtc cttggcctac cgacaattct cttttttgac ggacaaggcc aggagcatcc
    10861 acaagcacgc gtcacgggct ttatggatgc tgaaaccttc agcgcacatt tgcgcgatcg
    10921 ccaaccgtga acgacacttg cagcgggaaa gacggaggaa aatagcgtgc aacgtgaaga
    10981 tgtactggga gaagccctga aattattaga attacaaggg attgccaaca ccacgctgga
    11041 gatggttgct gaacgtgtgg attatccact ggacgagcta cgccgcttct ggccagacaa
    11101 agaggcaatc ctctacgatg cgctgcgtta tcttagccaa cagatagatg tctggcgtcg
    11161 tcagctgatg ctggacgaaa cgcaaaccgc cgaacaaaag ctgctggcac gttatcaggc
    11221 gttatcggag tgcgttaaaa acaaccgcta tccgggctgt ctatttatcg ctgcctgtac
    11281 gttttatccc gatcctggcc accctatcca tcaactggcc gatcagcaaa aaagcgcggc
    11341 ctacgatttc acccacgaac tgttaaccac actggaagtt gacgatccgg cgatggtagc
    11401 aaagcagatg gaactggtgc tggaaggctg tttaagccga atgctggtga atcgtagcca
    11461 ggcggatgtc gacactgcac atcggctggc ggaagatatc ctgcgcttcg cccgctgccg
    11521 ccagggtggt gcattgacct gacagaaaca cagaaaagaa gcgatttgcc gcaatcttaa
    11581 gcagttgaat cgcttttact gaaattaggt tgacgagatg tgcagattac ggtttaatgc
    11641 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
    11701 gttcgattcc gagtccgggc accaaattca tatcaacgga cctctacgga ggtccgtttt
    11761 tcgtttcaga acgccacgat ttaagcgttc tgcctacaaa tcaattctac cgaactcaac
    11821 cagattcccc ccacatcaac cccattgtgt gggtataatt gcgggcatac cccagttcga
    11881 cagaatttgt accctcttta acgcattcag aaggctgaac tatggcactg actgacgcaa
    11941 aaatccgggc tgcaaagccc actgacaagg cttataaact cactgacggg gctggcatgt
    12001 tcctgctggt acatcctaat ggctcccgtt actggcgtct ccgttatcgt attctgggta
    12061 aggagaagac tctggcactt ggtgtgtatc cagaagtttc tctctccgaa gctcgtacaa
    12121 aacgggatga ggcccgaaaa ctgatttcgg agggggttga cccttgcgaa cagaaaagag
    12181 ctaaaaaagt agtccctgat ttacagctct cttttgaaca tattgcgcga cgctggcatg
    12241 ccagtaataa acaatgggca caatcacaca gcgataaagt actcaaaagc ctcgagactc
    12301 acgttttccc ctttatcggc aaccgggata tcacaacact caataccccg gatctgctta
    12361 tccctgttcg tgctacagaa gctaaacaaa tttatgaaat cgccagtcgt ctgcagcaaa
    12421 gaatatctgc cgtaatgcgt tatgccgtac agtctggcat catcagatat aatcctgctc
    12481 tggatatggc tggcgcattg actacggtaa aacgccagca tcgccccgct cttgatcttt
    12541 ctcgcctgcc tgaacttttg tcgcgtattg gcagttacaa agggcaacct gtcacccagc
    12601 ttgccgttat gctgaattta ctggttttta ttcgttccag tgaactcaga tacgcccgct
    12661 ggtctgaaat tgatattgac aatgccatgt ggactattcc agccgaacgc gaacccctgc
    12721 caggcgtaaa attctcacac cggggctcca agatgcgaac accacatctt gtgccactca
    12781 gcaaacaggc tgtagccata ctgacagaac tacagacatg ggctggtgaa aatggtctga
    12841 tctttacggg agcacatgac ccgcgtaaac caatcagtga aaatgtaatg aacgcatc
//
LOCUS       DAJTMK010000115        12873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTMK010000115 DAJTMK010000000
VERSION     DAJTMK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12873
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.116"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..383)
                     /locus_tag="OO135_002818"
     CDS             complement(<1..383)
                     /locus_tag="OO135_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398605.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKHE"
     gene            complement(774..2042)
                     /locus_tag="OO135_002819"
     CDS             complement(774..2042)
                     /locus_tag="OO135_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5398606.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQRRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            complement(2243..2327)
                     /locus_tag="OO135_002820"
     tRNA            complement(2243..2327)
                     /locus_tag="OO135_002820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2291..2293),aa:Leu,seq:caa)
     gene            2523..3542
                     /gene="ahr"
                     /locus_tag="OO135_002821"
     CDS             2523..3542
                     /gene="ahr"
                     /locus_tag="OO135_002821"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5398607.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDHSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            3672..5174
                     /locus_tag="OO135_002822"
     CDS             3672..5174
                     /locus_tag="OO135_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5398608.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(5335..6417)
                     /gene="lptG"
                     /locus_tag="OO135_002823"
     CDS             complement(5335..6417)
                     /gene="lptG"
                     /locus_tag="OO135_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5398609.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQMKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6417..7517)
                     /gene="lptF"
                     /locus_tag="OO135_002824"
     CDS             complement(6417..7517)
                     /gene="lptF"
                     /locus_tag="OO135_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5398610.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7784..9295
                     /gene="pepA"
                     /locus_tag="OO135_002825"
     CDS             7784..9295
                     /gene="pepA"
                     /locus_tag="OO135_002825"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5398611.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9455..9898
                     /gene="holC"
                     /locus_tag="OO135_002826"
     CDS             9455..9898
                     /gene="holC"
                     /locus_tag="OO135_002826"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5398612.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9898..12753
                     /gene="valS"
                     /locus_tag="OO135_002827"
     CDS             9898..12753
                     /gene="valS"
                     /locus_tag="OO135_002827"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5398613.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAIIAKEREKLEGYAEAKAKLIEQQAVIAAL"
BASE COUNT         3152 a         3317 c         3424 g         2980 t
ORIGIN      
        1 atttcatgtt ttaactcatc attatctata atatttttat gtgcattctc aatatattta
       61 gatattgaca acttcaaagg agactgttcg ttaatgaaag ttttctcctg ttctttttca
      121 cttaatgcta attgtccttc ttcagtatcc caccatttct cagcatatgc agacaccaat
      181 aattgggcat attcgggtaa gtcaccatta gattttgttt ttaattttat atgattttcc
      241 ccaattgcct cagcaaaatg cttcaaaagg tcttgccaac atggcacacc aagataacga
      301 cgagacacac cggagccgac aaataacact ggggatgctt cgaactgctt aaaatgctct
      361 tcgagttttt tcttaaaatt cacacggata ctctctttaa aagaaatcaa gcccctttat
      421 actgattaca ttaagatatt gcggcttaca ccaccagtaa attggtcaat tttacgtaag
      481 aaatttaatt atttagattc aattaattat tagattaact aattgactat cactcctgat
      541 gtagccgaat tgctgtacct ttgttacgat gtagggctca atcaagggtc cactcccctt
      601 ttataagtaa tcatgacttt aaatttaggc ttaggtggag cattactgcc gattaggttc
      661 aataaaaagg cttaccttag tgaaaactcg gctatttggc actgctgacc aattcctcgt
      721 ttgaataagt cagcagcttt caatttagag gcgaatgtgc gcttttagat aactcactcg
      781 ccattacccc gattgatctt cgcatagtca aacggcgtaa tccccttctc ccggttcgca
      841 tccagaaaat cagcccacca ctgcaacatc agcttgcgct catccagatg ttccgcttta
      901 tggatatacg ccgccctcac cgaattgcgt tccatatggc tcatctgcct ttccaccgca
      961 tccttcgacc ataaccctga ctcaatcaat gagctacagg ccatagtgcg gaagccgtgg
     1021 ccgcaaacct caaccttggt gtcatagccc atcacccgca atgcactgtt caccgtgttt
     1081 tcactcatgg gcttactcgg atcgtgatcg ccaataaaaa tcagctcatg ttcgccacag
     1141 aactgtttta tctgcttcag gatcgctagc gcctgctttg aaagcggcac cagatgaggt
     1201 gtacgcatct ttgctcctcg ctgagaatgt ttcacaccgg gaataggttc tcgctcaggc
     1261 gggatggtcc acattgacgt ttcaaaatcg atttctgacc agcgagcaaa acgcagctca
     1321 ctggatcgaa taaaaataag cagggtaagt tcggttgccc agcgggttag tggcctgccg
     1381 gtgtattcat cgattttctg gagtaattcc gggatacgct ttaactccag cgccggacga
     1441 cgctgtcgat tactggaagc taccgccccc gccatctctt gcgccggatt gtaatcaatt
     1501 aacccgcttt gcactgcata acgcatgatg gccgtggtgc gctgttgaag gcgggaagcc
     1561 acttcaagac gtccggattt ctccacggct ttaataggaa ttaacagatc gcgggtgccc
     1621 agttcagcaa tattgtggga accaattgcc gggaagatat tgtcctccag gctctttttt
     1681 acacgattag catgctcttc cgaccatttt tggttggtta cgagccactc tctggcaacc
     1741 ttctcgaaag taataatctc tttcgcctgc tcttctttca cagcacgctt atgttcacga
     1801 ggatcgatac cagcagctac cagctttctg gcctcttccc ttttcttccg cgcatccgcc
     1861 agcgaaacat ccgggtaaac gccgaacgcc atcaaatgtt gtttaccgcc aaaacggaaa
     1921 cgaaatcgcc aatactttga accgccaggt tttacaagta aggacatgcc gtcgccgtca
     1981 acaagtgaat actctttctc ctcaggtttc gcagagcgaa ctttagtatc cgttagggcc
     2041 ataatggtct tccttccatt ggtatctcgt gagtatacaa gcaatatcga atcggcaaat
     2101 atactcatta gtatactcac aagcctgttg atgtgggttg agtcaggttg acttcagttg
     2161 aggggaaaag ctggaaaagc cttgtgtagc gcggatttca ggcataaaaa aagacctcag
     2221 ttgaggtcta tttacatact tttggtgccg aaggccggac tcgaaccggc acgtatttct
     2281 acggttgatt ttgaatcaac tgcgtctacc gatttcgcca cttcggcact gaagaggtat
     2341 gcggaaaacg ttgtggatta tacctgtcgc acgccaccat gcaagcgcca gcttgctcgc
     2401 acctcgctaa gtgctgaaat tttcagcatt gcacacagcg atgtgtaacc tttgtcacac
     2461 tccaggcacc ccgccctgcc atgctctaca cttcccaaac aacaccagag aaggaccaaa
     2521 aaatgtcgat gataaaaagc tatgccgcaa aagaagcggg cggcgaactg gaagtttatg
     2581 agtacgatcc cggtgagctg aggccacaag atgttgaagt gcaggtggat tattgcggga
     2641 tctgccattc cgatctatcg atgatcgaca atgaatgggg attttcacaa tatccgctgg
     2701 ttgccgggca tgaggtgatt gggcgcgtgg tggcactcgg gagcgccgcg caggataaag
     2761 gtttgcaggt cggtcagcgt gtcgggattg gctggacggc gcgtagctgt ggtcactgcg
     2821 acgcctgtat tagcggtaat cagatcaact gcgagcaagg tgcggtgccg acgattatga
     2881 atcgcggtgg ctttgccgag aagttgcgtg cggactggca atgggtgatt ccactgccag
     2941 aaaatattga tatcgagtcc gccgggccgc tgttgtgcgg cggtatcacg gtctttaaac
     3001 cactgttgat gcaccatatc actgctacca gccgcgttgg ggtaattggt attggcgggc
     3061 tggggcatat cgctataaaa cttctgcacg caatgggatg cgaggtgacg gcctttagtt
     3121 ctaatccggc gaaagagcag gaagtactgg cgatgggtgc cgataaagtg gtgaatagcc
     3181 gcgatccgca ggcactgaaa gccctgtcgg ggcagtttga tctcattatc aatactgtga
     3241 acgtcagcct cgactggcag ccttattttg aggcgctgac ctatggcggt aatttccata
     3301 cggtcggtgc ggttctcacg ccgctgtctg ttccggcctt tacgttaatt gcgggcgacc
     3361 acagcatctc tggttctgct accggcacgc cttatgagct gcgaaagctg atgcgctttg
     3421 ccgcccgtag caaggttgcg ccgaccaccg aactgttccc gatgtcgaaa attaacgatg
     3481 ccatcaagca tgtgcgcgac ggtaaggcgc gttaccgcgt ggttctgaaa gccgacttct
     3541 gatccctttg ccgggtgcct gtcgcccggc acttaaatct ctttggaagg cgtctcgcaa
     3601 tctgctgaat ttagcggtga agagtgcgca gccgttgcct atactccagg caggaaactg
     3661 gaggaaatct catgagcgaa cccctattaa ttgcccgcac gccggacaca gaactttttt
     3721 tactgccggg aatggctaac cgtcacgggc tgattactgg cgcaacgggg acgggtaaaa
     3781 ccgttacgct gcaaaaactg gcagagtcat tgtcggaaat cggcgtgccg gtgtttatgg
     3841 ctgatgtgaa aggcgatctg accggtatcg cgcaggcagg aacggcgtcg gaaaaactgc
     3901 tcacaaggct taaaaatatc ggcgtcaatg actggcaacc gcatgctaat ccggtggtgg
     3961 tgtgggatat ctttggcgag aaaggccatc cggtgcgggc gacggtttca gacctggggc
     4021 cgctgttgct ggcgcggctg ttgaatctca acgatgtgca atctggcgtg ctgaatatca
     4081 tcttccgtat tgctgacgat cagggattgt tgctgctcga ctttaaagat ctgcgggcga
     4141 ttacccagta catcggcgat aacgccaaat ctttccagaa tcagtacggt aatatcagta
     4201 gcgcatcggt tggtgccatc cagcgcggtt tactgtcgct ggaaaagcaa ggtgcggcgc
     4261 atttctttgg cgagccgatg ctggatatca aagactggat gcgcaccgat accaacggta
     4321 aaggcgttat caatatcctc agcgccgaga agctttatca gatgccgaaa ctgtacgccg
     4381 ccagcctgct gtggatgctt tcagagttgt atgaacaatt gccggaagcg ggcgatctgg
     4441 agaaaccaaa actggtgttt ttcttcgacg aggcacatct gctgtttaac gatgcaccgc
     4501 aggtactgct ggataagatt gagcaggtaa taaggcttat tcgctcaaaa ggcgtgggcg
     4561 tctggttcgt ttcgcaaaac ccgtctgata ttccggataa tgtgctcggg cagctcggta
     4621 atcgcgttca acacgctttg cgggctttta cgcccaaaga tcagaaagcg gtaaaagctg
     4681 cggcgcaaac catgcgggcc aatccggcgt ttgataccga aaaggcaatc caggaactgg
     4741 ggaccggcga ggcgttaatc tcgtttctcg atgcaaaagg aagtccttct gtggtggaac
     4801 gggcgatggt gattgcgcct tgttcgcgga tgggaccggt gacggaagat gagcgcaatg
     4861 gcttgattaa ccactccccg gtgtatggca agtacgagga tgacgtggac cgggaatccg
     4921 cctatgagat gttgcaaaaa ggctttcagg ccagtaccaa gcagcaaaat aatcctcccg
     4981 cgaaagggaa agcggtggcg gtggatgacg gtattcttgg tggattgaag gatattttgt
     5041 ttggcactac cggaccacgc ggcgggaaga aagatggtgt ggtgcaaaca atggcgaaaa
     5101 gcgccgcccg ccaggtgacg aatcagattg tacgtgggat gttggggagt ttgctggggg
     5161 gaagaagaag gtaagtcagg cgcgatttgt ctttgcctga tgcgacgctg gcgcgtctta
     5221 tcatgcctac accactgcaa tatattggat tttcatgatt tttcaggcat gaacgaagca
     5281 cactttgcta acaataataa agggagcttt cgctcccttt attcgttcat tcggttacga
     5341 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
     5401 gatgatcggc gggatgccat aaaccaacgt cagcgggccg aagatctggt ccagtacgta
     5461 gaagacaaaa ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag
     5521 tgggccaaag atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa
     5581 gattttgctc cacatattga gctgataacg tccggcatcc tgaccgctcg acttcagata
     5641 cttcacgtag ttgtgcaaac cgctgattga gagtgcatcc gggtccagcg ccaccacgcc
     5701 tagtttgtcc ggcgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtgat
     5761 ctgtttcgga ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc
     5821 cgggtcaaac ttcgcagtag cggcatagcg tacggattgc agacgacgat tctcgttaaa
     5881 ggcataaata ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtaaacgaa
     5941 gttgttgcca tctttcgccc ataagccttg ctgggtagag agcaacgagc cgccgtacat
     6001 cgcctgcgca cggtagttac gcgccatctg ctcgccctgc ggcgcgaccc attcaccaat
     6061 cgccatcgtc agcaagacca gcggaatggc ggttttcatc accgacagcg caacctgcat
     6121 acgggtgaaa ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag
     6181 accaagcaac gccccaagca gagccgccat cgggaagaat gtttgcacat ctttcggcac
     6241 gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt
     6301 catctgatcg acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat
     6361 gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac
     6421 accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg
     6481 ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca cagcgtcggg
     6541 tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga
     6601 tacagcagca tggctggcag catcgatagt acgcgtccct gacgcgggtt aaccacgctc
     6661 agcggtacga ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc
     6721 agttctgcgc gggcacgatc ggtgtcagtg ttccacaatg tacgcatgtc catctggtcg
     6781 gtatcgttcg ggtcgagcgc taccgcctgg tgaccaatga tcgcctgata atcctggaag
     6841 tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga
     6901 gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca
     6961 gaaggacgtg cattaccttt tggtcgaatt tgcgcgagga acacatcttt gaaatcgctg
     7021 ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct
     7081 tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga
     7141 cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg cgacgattgc agtgaatacc
     7201 gcaaggatca ttgccgcttt caccagaacc gctttgctca ggccgcaggc atgcattacc
     7261 gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt
     7321 aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga
     7381 ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag
     7441 aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga
     7501 tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc
     7561 ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa
     7621 ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt
     7681 taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat
     7741 ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa
     7801 aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg
     7861 tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct
     7921 gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc
     7981 gaatgtactt tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga
     8041 gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcaat
     8101 ggaagcggtc tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt
     8161 gcgtcaggct gtcgagacgg caaaagagac gctctacagt ttcgatcagc tgaaaacgaa
     8221 caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga
     8281 actgaccagc ggtgagcgcg cgatcctgca cggtctggcg attgccgccg ggattaaagc
     8341 agcaaaagat ctcggcaata tgccgccgaa tatctgtaac gccgcttacc tcgcttcaca
     8401 agcgcgccag ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca
     8461 gcagatgaaa gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcaaaacga
     8521 atcgctgatg tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt
     8581 gctggtgggt aaaggtttaa ccttcgactc cggcggtatc tcgatcaagc cttcagaagg
     8641 catggatgag atgaagtacg atatgtgcgg tgcggcagcg gtttacggcg tgatgcggat
     8701 ggtcgcggag ctacaactgc cgattaacgt tatcggcgtg ttggcaggct gcgaaaacat
     8761 gcctggcgga cgagcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaactgt
     8821 tgaagtgctg aataccgacg ctgaaggccg cctggtactg tgcgacgtgt taacttacgt
     8881 tgagcgtttt gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgtgat
     8941 cgcgctgggt catcacatta ccggtctgat ggcgaaccat aatccgctgg cccatgaact
     9001 gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta
     9061 tcaggaacag ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg
     9121 ggcgattacc gcaggttgct tcctgtcgcg ctttacccgt aagtacaact gggcgcacct
     9181 ggatatcgcc ggaaccgcct ggcgttctgg taaagcaaaa ggcgccaccg gtcgtccggt
     9241 ggcgttgctg gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaattgcg
     9301 tcaggcaagg ctgttattgc cggatgcggc gtgaacgcct tatccgacct acacagcact
     9361 gaactcgtag gcctgataag acacaacagc gtcgcatcag gcgctgcggt gtatacctga
     9421 tgcgtattta aatccaccac aagaagcccc atatatgaaa aacgcgacgt tctaccttct
     9481 ggacaatgac accaccgtcg atggcttaag cgccgttgag caactggtgt gtgaaattgc
     9541 cgcagaacgt tggcgcagcg gtaagcgcgt gctcatcgcc tgtgaagatg aaaagcaggc
     9601 ttaccggctg gatgaagccc tgtgggcgcg tccggcagaa agctttgttc cgcataattt
     9661 agcgggagaa ggaccgcgcg gcggtgcgcc ggtggagatc gcctggccgc aaaaacgcag
     9721 cagcagcccg cgcgatatat tgattagtct gcgaacaagc tttgcagatt ttgccaccgc
     9781 tttcacagaa gtggtagact tcgttcctta tgaagattct ctgaaacaac tggcgcgcga
     9841 acgctataaa gcctaccgcg tggctggttt caacctgaat acggcaacct ggaaataatg
     9901 gaaaagacat ataacccaca agatatcgaa cagccgcttt acgagcactg ggaaaagcag
     9961 ggctacttta agcctaatgg cgatgaaagc caggaaagtt tctgcatcat gatcccgccg
    10021 ccgaacgtca ccggcagttt gcatatgggt cacgccttcc agcaaaccat catggatacc
    10081 atgatccgct atcagcgcat gcagggcaaa aacaccctgt ggcaggtcgg tactgaccac
    10141 gccgggatcg ctacccagat ggtcgttgag cgcaagattg ccgcagaaga aggtaaaacc
    10201 cgtcacgact acggccgcga agctttcatc gacaaaattt gggaatggaa agcggaatcc
    10261 ggcggcacga ttacccgtca gatgcgccgt ctcggcaact ccgtcgactg ggagcgtgaa
    10321 cgcttcacga tggacgaagg cctgtccaat gcggtgaaag aagttttcgt tcgtctgtat
    10381 aaagaagacc tgatttaccg tggcaaacgc ctggtaaact gggatccgaa actgcgcacc
    10441 gctatctctg acctggaagt ggaaaaccgc gaatcgaaag gttcgatgtg gcacatccgc
    10501 tatccgctgg ctgacggcgc gaaaaccgca gacggtaaag attatctggt ggttgcgact
    10561 acccgtccgg aaaccctgtt gggcgatacc ggcgtggccg ttaacccgga agatccgcgt
    10621 tacaaagacc tgattggcaa atatgtcatt ctaccgctgg ttaaccgtcg tattccgatc
    10681 gttggcgacg aacacgccga catggaaaaa ggcaccggct gcgtgaaaat cactccggcg
    10741 cacgacttta acgactatga agtgggtaaa cgtcacgcgc tgccgatgat caacatcctg
    10801 acctttgacg gcgatatccg tgaaagcgcc caggtgttcg ataccaaagg taacgaatct
    10861 gacgtttatt ccagcgaaat cccggcagag ttccagaaac tggagcgttt tgctgcacgt
    10921 aaagcagtcg ttgctgcggt tgacgcgctc ggcctgctgg aagaaattaa accgcacgac
    10981 ctgaccgtgc cttacggcga ccgtggcggc gtagttatcg aaccgatgct caccgaccag
    11041 tggtacgtgc gtgccgatgt actggcgaaa ccggcggttg aggcggttga gaacggcgac
    11101 attcagttcg taccgaagca gtacgaaaac atgtacttct cctggatgcg cgatattcag
    11161 gactggtgta tctctcgtca gctgtggtgg ggtcaccgta tccctgcatg gtacgacgaa
    11221 gcaggtaacg tttatgttgg ccgcaacgaa gaagaagtgc gcaaggaaaa taacctcggt
    11281 gccgacgttg ccctgcgtca ggacgaagac gttctcgaca cctggttctc ttctgcgctg
    11341 tggaccttct ctaccctagg ctggccggaa aacaccgacg cactgcgcca gttccaccca
    11401 accagcgtga tggtatctgg tttcgacatc attttcttct ggattgcccg catgatcatg
    11461 atgaccatgc acttcatcaa agatgaaaat ggcaaaccgc aggtgccgtt ccacaccgtt
    11521 tacatgaccg gcctgattcg tgacgacgaa ggccagaaga tgtccaaatc caagggtaac
    11581 gttattgacc cgctggatat ggttgacggt atttcgctgc cggaactgct ggagaaacgt
    11641 accggcaata tgatgcagcc gcagctggcg gacaaaatcc gtaagcgcac cgagaaacag
    11701 ttcccgaacg gcattgagcc gcacggcacc gatgccctgc gcttcaccct ggcggcgctg
    11761 gcttctaccg gtcgtgacat caactgggat atgaaacgcc tggaaggcta ccgtaacttc
    11821 tgtaacaagc tgtggaacgc cagccgcttt gtgctgatga acacggaagg tcaggattgc
    11881 ggcttcaacg gtggtgaaat gacgctgtcg ctggcggacc gctggattct ggcagagttc
    11941 aatcagacca tcaaagcgta ccgcgaagcg ctggacagct tccgcttcga tatcgctgca
    12001 ggcattctgt atgagttcac ctggaaccag ttctgcgact ggtatctgga gctgactaag
    12061 ccggtaatga acggtggaac cgaagccgaa ctgcgcggta ctcgccatac gctggtgact
    12121 gtactggaag gtctgctgcg cctcgcgcat ccgatcattc cgttcatcac cgaaaccatc
    12181 tggcagcgtg tgaaagtact ttgcggtatc actgccgaca ccatcatgct gcagccgttc
    12241 ccgcagtacg atgcatctca ggttgatgaa gccgcactgg ctgacaccga gtggctgaaa
    12301 caggcgatcg ttgcggtacg taacatccgt gcagaaatga acatcgcgcc gggcaaaccg
    12361 ctggagctgc tgctgcgtgg ttgcagcgcg gatgcagaac gtcgcgtaaa tgaaaaccgt
    12421 ggcttcctgc aaaccctggc gcgtctggaa agcatcaccg tgctgcctgc cgatgacaaa
    12481 ggtccggttt ccgttacgaa gatcatcgac ggcgcagagc tgctgatccc gatggctggc
    12541 ctcatcaaca aagaagatga gctggcgcgt ctggcgaaag aagtggcgaa gatcgaaggc
    12601 gaaatcagcc gtatcgagaa caagctggcg aatgaaggct ttgtcgcccg cgcaccggaa
    12661 gcgatcatcg cgaaagagcg tgagaagctg gaaggctatg cagaagcgaa agcgaagctg
    12721 attgaacagc aggctgttat cgccgcgctg taatcaaaac acagtgatga aaacgaaggc
    12781 cggagcatgc tccggccttt tttatctctt acagaaatgg taaagaaggc ataattcccc
    12841 gtgaaatact ggctaagaag cctttatctg gtt
//
LOCUS       DAJTMK010000116        12790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTMK010000116 DAJTMK010000000
VERSION     DAJTMK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.117"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..194
                     /locus_tag="OO135_002828"
     CDS             <1..194
                     /locus_tag="OO135_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5398614.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            184..357
                     /locus_tag="OO135_002829"
     CDS             184..357
                     /locus_tag="OO135_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5398615.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(406..1479)
                     /locus_tag="OO135_002830"
     CDS             complement(406..1479)
                     /locus_tag="OO135_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5398616.1"
                     /translation="MAEKKRTRWQRRPGMTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1598..3352)
                     /gene="torS"
                     /locus_tag="OO135_002831"
                     /pseudo
     CDS             complement(1598..3352)
                     /gene="torS"
                     /locus_tag="OO135_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            3435..4424
                     /gene="torT"
                     /locus_tag="OO135_002832"
     CDS             3435..4424
                     /gene="torT"
                     /locus_tag="OO135_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5398617.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQDMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMVWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(4397..5089)
                     /gene="torR"
                     /locus_tag="OO135_002833"
     CDS             complement(4397..5089)
                     /gene="torR"
                     /locus_tag="OO135_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5398618.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            5209..6381
                     /gene="torC"
                     /locus_tag="OO135_002834"
     CDS             5209..6381
                     /gene="torC"
                     /locus_tag="OO135_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5398619.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDSHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITCWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCYG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            6381..8961
                     /gene="torA"
                     /locus_tag="OO135_002835"
                     /pseudo
     CDS             6381..8961
                     /gene="torA"
                     /locus_tag="OO135_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            8958..9557
                     /gene="torD"
                     /locus_tag="OO135_002836"
     CDS             8958..9557
                     /gene="torD"
                     /locus_tag="OO135_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5398620.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(9650..9955)
                     /gene="cbpM"
                     /locus_tag="OO135_002837"
     CDS             complement(9650..9955)
                     /gene="cbpM"
                     /locus_tag="OO135_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5398621.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(9955..10875)
                     /gene="cbpA"
                     /locus_tag="OO135_002838"
     CDS             complement(9955..10875)
                     /gene="cbpA"
                     /locus_tag="OO135_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5398622.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11136..12391
                     /locus_tag="OO135_002839"
                     /pseudo
     CDS             11136..12391
                     /locus_tag="OO135_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
BASE COUNT         3229 a         3102 c         3287 g         3172 t
ORIGIN      
        1 ctcgtggagc cttttttata tctgccttat ttttcttcaa cgctgtatgt atagtaagcg
       61 ataacctgtt gattattgaa tctttcgggg agatggctta taacatttct tacctgacca
      121 gggtaccggg aaccaacacc ttactggcgt gttgctgtct tttaagacca gaagaggtta
      181 acagtgaata ttgaagagtt aaaaaaacaa gccgaaacgg aaatcgccga ctttatcgcg
      241 caaaaaattg ccgagctgaa caagaataca gggaaagaag tctctgaaat tcgcttcacc
      301 gcacgagaaa aaatgaccgg gcttgaaagt tatgatgtca aaatcaaaat aatgtgattt
      361 tgtgaacatc accccgtgcg aggtgatgtt ccgcttgttg ctaatttagt gaccaatcat
      421 tggcgcttgt ggaattaagc gtcggtacaa ttcctccggc accgggcttt gccatactcc
      481 cgcatacatt gcgtaaccaa tcaccgcgaa cataatcccc agaaccagta gcgtcattaa
      541 ccagccagac aacgcaaaag cttttttatt tgccgcaggt ttttgcagtg aaaaggtcaa
      601 tgttgaggct accggacatg actctacgca agtcatacag ccggtacatt ccactgttcg
      661 tacctgaatt aatttatcta ccgggatccg tgatgggcaa ttttttgcgc atttgccaca
      721 gccgatacaa ctttcggcat tgcgacgaat cttaaacggc gataatagcg aaaccacgcc
      781 catcagcgcg ccatatgggc aaagataacg acaccaggca tggcgaataa acaggctggc
      841 aatcagcaaa acggtcacgc tgattaatgt cgcggtcccc atatgacgaa agaaatcgag
      901 catttttacg tccatcacca cgctgtaggg cgacaacata aaatagtgaa tcgcctgagc
      961 gggcatcaat aacgcgatat agagaaaaaa actcaacagc aaatacttca cgccgcgcag
     1021 aggaatatcc agccagcggg gaaggacaca ttgccgacca aacagtttgt taccgagatc
     1081 gccgattaat tcagaaagcg taccaaccgg gcataaccat gagcaaaagg cctttttgag
     1141 taatagactg atgacgataa aagcgaccaa taacagcatc gcggcggcgt ggacggacgg
     1201 taactgacct gttgtaaggc tatatttcag attcatcaga ccggcaatcg gtagccagcc
     1261 ttcgatacct cccggtctgg cgacaaatgt cgtgctactt gccgtttcgt aatagcgcac
     1321 ccaataccag aacgtgatgg caatataaat attcattgcc aacagtaata attgcgtcgc
     1381 tttacgccag gtcgtggcat tgcgccagtc attccacggt aatttgccgc ccgtcatgcc
     1441 tggccgccgc tgccagcggg ttcttttttt ctctgccatg attttgccag tccgttaagt
     1501 tgcataccaa atgccactat tctagttgtt cttaactggc tgatattgat tcaaatcgcg
     1561 ttcaggtctt tcttatgcaa ccatgcttcc agagcggcaa cactgcgtgt tgcccttcgt
     1621 cagtaaaacg cagggcgttg ctcaacaggt tggttataac ctgacgaata cgtcgcggat
     1681 cgcccattaa cgcggtcggt acatcgtcgg caattgctgt tgccaggcga atcgggcgac
     1741 ctttcacccg tccgctcatt aattgcaggg tactttccag cagcgggcgc ggttcaaagg
     1801 gctcatcgct gaccgaaaca ttcttgccac ctgcttcgat agccgaataa tcgagaatat
     1861 cgttgaggat ggtcagcaac gattcaccag agtcggtaat cgcccgcaaa tcatcacgct
     1921 gggcgttaag tgcggggtta tctgccagca gttgagcagt gccgagaata ccgtacagcg
     1981 gtgtgcggat ctcatggctc atcgccgcca gaaaggccga ttttgcctgg ctggcttttt
     2041 ctgcttccgc ccgtgcctgt cggtgttcta tcaccagttc ctgcaattca gctgtacgcg
     2101 ctttgacctg cgccgccagc tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa
     2161 acgcatccat cagccgcccg atggtatcca gctcccgtac gccagcggtt tccgggaaag
     2221 gggagtcaat atcaccgtcc agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac
     2281 gcgtgactga gcgataaacc acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca
     2341 tccccagcaa taacaggctg tattgcccgc gtgcactggc tttttccaga tgcgccagtc
     2401 cgtgctgatt acgcagctca atggtgtcga ccagctgact gacttcgcta ctaaactgcg
     2461 cgaacagggc gatgttattt tgtgcgagag tttgtaggtg attgctgatt tcactgtcct
     2521 gctgatacag cgccagcaaa tcgctatatt ggctaacggt agttaacgtt gttgcgacct
     2581 gcgcacgaac acccggatct tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat
     2641 tattgagctg cttttccagc gttggtgcat ttttctggat ctgctccagc cccagattca
     2701 tcaccatttg ctgcacccgc agagcgctaa ggcgcagttc attcatctgg ttaacatact
     2761 caagatcgat atcaatcagc cgatcgagtg cactttcagc agcctgacgc tgatcttgtt
     2821 cgatcaaatc gtaaatcccg gccagggtcg ctccagcgga agttgtcgca ttattcgcct
     2881 gaccttgcgc caggcgtgcg atctcatcgg cggcagcgac tatctgctga ctgagttgct
     2941 gttgttgctg gcgtagttgc agacgccgcc ccaccagttc cccttgctga cgtaacgaac
     3001 gggagatctc ctgctcctgt tgttcaatag cggtggtatc aaaaccttgt tcctgtaacg
     3061 cttgcagcaa cgcattaatc ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc
     3121 acatcttttc gttatcggca ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg
     3181 cttcgctcaa ctggcgcgcc atattcatgg taggaatcaa tgcctgagtg ttgtcttttt
     3241 ccacctggct gataaagcgc aggttgtacc atcccaccag ggtactggtc agggttaaca
     3301 gcgccatcag ggcaaagccc atccagagtc ttcgggtcag ggttaaattc acggtcggtg
     3361 cactttaggt gaaaaaggtt gagtcgcaaa gcagaatgca tctagcataa agccttatta
     3421 ttgatgaggc tatcatgcgc gtactgctat ttttacttct ttcccttttc atgttatcgg
     3481 cattttcggc tgataacctg ttgcgctggc atgatgcgca gcattacacg gtgcaagcct
     3541 ctacgccgct taaagccaaa cgcgcatgga aactgtgcgc gctttatccc agcctgaaag
     3601 attcatactg gttatcgttg aactatggta tgcaggaggc aggcggctac agccagttgg
     3661 ctacccagca agcacaaatc gaccagtgta aacagtgggg cgcagaggcc attttgctcg
     3721 gtagtagcac tacgtcattt cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg
     3781 aactggtaaa tgctcttgat gatcccctgg tgaaaagccg cgttggtgta ccctggtttc
     3841 agatgggcta tcaacctggg cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg
     3901 tgctgttgat gcccggaccc gataatgccg ggggcagtaa agagatggtc gagggttttc
     3961 gcgcagccat tgccggaagc ccggtgcgta ttgttgatat tgcgcttggt gataacgata
     4021 ttgaaatcca gcgtaacctg ttgcaggata tgctggaacg ccacccagaa atcgacgtcg
     4081 ttgccggaac ggccattgcg gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc
     4141 cgcttaccgt ggtgtcgttt tatctttcac atcaggtgta tcgcgggctg aagcggggaa
     4201 gagtgattat ggctgccagc gatcaaatgg tctggcaggg ggaactggcg gttgagcagg
     4261 ccatcaggca attacagggg caatcggtgt ctgataatgt cagcccaccg attttagttc
     4321 tgacgccgaa aaatgccgac cgtgaacata ttcgccgctc gctgtcacca gggggatttc
     4381 gtccggtcta tttttatcag cacacatcag cggctaagaa ataaccttca ccatgttgcg
     4441 tcaccagtaa atccgcgctg agtttatgac gtaaacgacg aattaacaca tcgacggtgc
     4501 gcaggtcagg gttttccacc cgacgcgcag aaagcatacg tagcagacgt tcacggctga
     4561 gaatttcgcc cggattcgtc acaaatgcca ccaacatttc atactctgcg cgggtcagtt
     4621 taatcggctc gccatcccgc tccagcgtat ggcgcgacac attcaggcaa taaccggcaa
     4681 agcgatagca gttgtcctga gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga
     4741 gcagattttt cacccgtact accagttcgc gcagttccag cggtttggtg acgtaatcgt
     4801 ctgcgcccat ttccagccca acaatacggt caatccgatc gctgcgtccg gtaaccagaa
     4861 taatccccac cgttgagcgt tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg
     4921 gtaagttgat atccagcaga attaaatcta ccggctgatt ctgcataatt tcccgcagcc
     4981 cggcaccgct cgctgtaacg gaaacggtat acccctcctg agtgaagtaa gattgtaatc
     5041 gcgcctgggt aaccggctca tcttcaacaa taacaatgtg atgtggcatc agagggtttt
     5101 actcattctg ttcatattct gccgtaagcc gttcatcctg accagtgccg ctgttcatat
     5161 ttgctcatta agatcgcttc actaaaccat aattttacag gggctattat gcggaaactc
     5221 tggaacgcgc tacgccgacc cagtgctcgt tggtcggtac tggcgctggt cgcaattggg
     5281 attgtgattg gcattgcgct gattgtattg ccacacgttg ggatcaaagt caccagcaca
     5341 accgaatttt gtgtcagttg ccacagtatg caaccggtgt atgaagaata taaacagtcg
     5401 gtgcatttcc agaacgcctc cggcgtgcga gctgaatgcc atgactgtca tatcccgccg
     5461 gatattccag gcatggtgaa gcgcaaactg gaagcgagca atgatatcta ccagaccttt
     5521 attgctcact ccattgatac acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag
     5581 cgtgaatggg cgcgaatgaa agaaaacaac tcggcaacct gccgctcctg ccataactac
     5641 gatgcgatgg atcatgcgaa gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg
     5701 aaagataatc aatcctgcat cgacagtcat aaaggtattg cccaccagtt accggatatg
     5761 agtagcggct tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac
     5821 acgctgtact ctattgatat taagccaatt tatgcggcga aaggcgataa agaagcctct
     5881 ggttctctgc tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa
     5941 attgaaatta cctgctggac ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca
     6001 ggtaaacgca tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg
     6061 gagaaaacca ccgttgccga caccaatacc gagtggagca agttgcaggc cactgcgtgg
     6121 atgaagaaag gtgatatggt gaacgatatc aaaccgatct gggcttatgc ggattcgttg
     6181 tacaacggca cctgtaacca gtgctacggc gcaccggaaa tctctcactt tgatgctaac
     6241 ggctggattg gcacgctcaa cggcatgatt ggctttacca gtctcgataa acgtgaagaa
     6301 cgcaccttgt tgaaatatct gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc
     6361 gataagaagg aagaaaaata atgaacaata acgatctctt tcaggcatca cgtcggcgtt
     6421 ttctggcaca actcggcggc ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc
     6481 cgcgcagtgc gactgcggcg caagcggcgg ctgtcatctc gaaagagggc attcttaccg
     6541 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     6601 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat tgccggattg
     6661 ccggattgcc ggattgccgg attgccggat cacgtacaca acgcggcgcg tattcgttat
     6721 ccgatggtac gcgtggactg gctgcgtaag cgccatctca gcgatacctc ccagcgcggt
     6781 gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg acatgttcta tgaagaactg
     6841 gaacgcgtgc agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatcg
     6901 acggggatgt tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat
     6961 agcgttggta cgggcggaga ttactctacc ggtgctgcgc aggtgatcct gccgcgcgta
     7021 gtcggttcga tggaagtgta tgaacagcaa acctcctggc cgctggtatt gcagaacagc
     7081 aaaaccatta tgctgtgggg ctccgatttg ctgaaaaacc agcaagcgaa ctggtggtgc
     7141 ccggatcacg atgtttatga atattacgag caactaaaag cgaaagtcgc cgccggtgaa
     7201 attgaggtca tcagcatcga tccggttgtc acatccaccc atgagtatct ggggcgcgag
     7261 catgtgaagc acattgcggt taacccgcaa actgacgtgc cgctgcaact ggcgctggcg
     7321 tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtaggt
     7381 tttgagcagt tcctgccgta tctgctgggt gagaaagacg gtcagccgaa agatgccgca
     7441 tgggctgaaa aactgaccgg cattgatgcc gaaaccattc gtgggctggc gcggcagatg
     7501 gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc agcgtatgca gcacggtgaa
     7561 cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg cctgccaggt
     7621 ggtggttttg gttttggctg gcactacaac ggcgcaggca cgccggggcg taaaggcgtt
     7681 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
     7741 aaaggctaca gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     7801 aaagtgatca actggaacgg taaatcggta aaactgccgc cactgaaaat gtgtattttt
     7861 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     7921 aagctggaaa cggttatcgc catagataac cagtggacct caacctaccg ctttgccgat
     7981 atcgtactgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
     8041 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgcagttcga ggcgcgcaac
     8101 gactttgata ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc ctttaccgaa
     8161 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
     8221 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
     8281 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
     8341 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
     8401 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
     8461 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
     8521 tcgcagttat gtgagtcgga aactctgcgt cagcaatata cggtagcggg taaagagcca
     8581 gtattcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
     8641 gtctttaacg ctcgcggtca ggtgttggca ggggcagtag tttctgaccg ctatgcaccc
     8701 ggcgtggcac gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
     8761 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcggtac ttcgcagctg
     8821 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggagcagtg
     8881 gagcaggtga cggcgtttaa cggccctgtg gagatggtgg cgcagtgcga atatgttccc
     8941 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
     9001 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
     9061 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctggct gcggcggtga
     9121 atgagctgga aaactgtatt gccacgctga cagtacgtga cgatgcccgt ctggaactgg
     9181 ccgctgactt ttgcggcctg tttttgatga ctgataagca ggccgcgctg ccgtatgcat
     9241 cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaaa
     9301 ccagcggcaa tttcaacgaa ccggcagacc atctggcgat ctatctcgag ttgttgagtc
     9361 atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac agtttgcggc
     9421 aaaaaacact gacggcgctg tggcaatggt taccagagtt tgctgcgcgt tgccatcagt
     9481 atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta gtggagtgcg
     9541 accaccaaaa caaataacgt cgtttatgcg cctgaaaaga cgcgtttagc gtcgcatcag
     9601 gcattatggc gcagttgccg gatgcgtcat cacatccggc aaacacacat cacggatgag
     9661 ctacaaaccg ggaaagccgc tggcgtagca ggcggttttc ctgcttcagg tgtgcaatat
     9721 catccattaa cgtcagcgcc accgcgatcc ccggccagtc cagagccagt tcatgacgca
     9781 ggcgtaccgc gcgttgcacc acaatggcgg catggtcgtc aaatacccag gttgtttcct
     9841 ggatctcacg cggttcaacc acccccaaac cgacaatttc attcaactcc tcttcagaga
     9901 tgccggtatg caggcaaaat tcggtaatag taaaagtcac cgtaacatta gccatcatgc
     9961 tttcccccaa tctttacgtg gatcaaaaga cgactgggcg tctgccagtt gctgccacag
    10021 cgcggcagtg ttttcatccg gtttcggcgg catcacgatt ttcagtaccg catacagatc
    10081 gccggtctgt tttttgctca ccagaccttt gcctttaacg cgcaatcgtt gcccggcctg
    10141 gctgcctggc gggatagtca gcaaaatgct ttctttcagt gttggaacgg tgactttagc
    10201 acccagcgcc gcttcccacg ggctaaccgg caccacaatt tccagatcct ggccgacaat
    10261 atcgaacagc ggatgtggcg caatatgaat caccagccac aaatcgccat ttggaccacc
    10321 gttttcgccc ggcgtcccct ggcctttcag acggatgcgt tgaccattgc cgacgcccgc
    10381 cgggatcttc acattcagcg ttttcgggat ttcctgttcg atcatgccaa aggcgttata
    10441 aaccggcagg ttatagctga tggtacgctt atgctcagtc agcgtttctt cgaggaatac
    10501 cgccacttcg atttcaatat cgtggccgcg tgcggcgggg cgttgacggc tctggcgggc
    10561 atgctgaccg aaaattgacg agaagatatc gtcaaaatct tcggcattaa aactctgacc
    10621 gtcgccatga tggaactgac ggttaaattg cggatcgttg cgatgttgcc acatctgatc
    10681 atactcagcg cgacgttgtt catcacttaa cacttcccag gcttcagcga cctctttgaa
    10741 gcgggcttcg gcatccggtt ctttgctgac atcaggatgg tatttacggg ccagtcgacg
    10801 ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc acgcccatga tggcgtaata
    10861 atcctttaat tccatagcgt tatctcgcgt aaatcaacac aaattgaagg aacccctgta
    10921 aggtaactcc tataagtgta gggtaatcct caaaatttca tatgccaaca cagaatatgt
    10981 tattgaaatc atcgtggaga ggaggtcgcc atcaatatgg gttgctgaac atattttaaa
    11041 caggtgaaaa agggtgagcg atttttgata gttgaactag gcactttaag tttaactagg
    11101 gcgtcattat ttattaaatt ttatagacgc tatatatggg tagtaatata catggaatta
    11161 gttgcactgc aaataattat ttgaaacagg cctggaacga tataaaaaat gagtacgaaa
    11221 aaaatcaaac atattcaatc acgctttttg aaaacacact ggtgtgtttt atgcggttat
    11281 acaatgaact cagacgtaaa gtaaatgaag aggatactcc atgtctggaa tgtgaatcac
    11341 tagaaaaaga atttgaggaa atgcagaatg ataatgatct atcattattt atgagaacat
    11401 tgcgtactaa tgatacacaa atttattcag ggtttcagga ggtattacat atactataca
    11461 atatgttcaa aatgttgata ttgttagagt gtccttgccg ggcagaggtt cagagtctat
    11521 cacagatttt aaaggttatt attggtatgg ctttatggaa tatattgaaa acattactgc
    11581 gtgtgatgat gttttttctg agtattgttt agatgatgaa aatatgagta tccagccaga
    11641 acagataaat acgccgggaa tatctgattt agatactggt attgatttgt ctggcatatc
    11701 ttttattcag agtgaaatta ataaaacata tggattaaaa tacgctccag tagatggcga
    11761 tggatattgt ctgttaagat ccatactggt tttaaaagaa catgagtatt catgggcgct
    11821 gggcggccat aagacgcaaa agcaagtata cgaagagttt attaaaattg ttgataaaca
    11881 aacgatcgag gctcttgttg atacggcatt ctataatctc agggaagatg taaagacgtt
    11941 atttggcgtt aatctacaat ctgacaacaa aattcagggg cagggtggtt ttttatcatg
    12001 gagttttttg tcttttaaaa aagaatttat tgatagttgc ttgaatgaca aaaaatgcat
    12061 actgcattta cccgaattta tatttaatga taacaaggta cgccttgttt tagacaccga
    12121 cccagagcag agggttaatg aggtgaaaaa tttcctcact gctctttcag atagcatttg
    12181 ctcattattt atttttaata gtaatatggc atcaatctcc ttggggaatg aatccgtttc
    12241 aacagatgac gaccttgagt atggttattt aataaacact ggcaatcatt atgatgttta
    12301 cctccctcct gaactttttg ctcaggctta taagttaaaa aataaggaaa tgaatgcgca
    12361 actcgactat ttaaatcgtt atgcaattta atggcaaagg catatgctaa aaaccattgt
    12421 tattagtctc acactttttt attggtaaat attgtctctg tattggtaac gccgcagata
    12481 ttctgtttag ccacaggtgc aattatcagc ggcgtacgcg aggcaggggc taatcaggca
    12541 tagtttgcgt caaaccttgc ctgtttttga agatgtatat agaaaaacag gcgttcaaca
    12601 agccattttg cgaacctgtt cccggaaaaa agtcatattt ctgtcacact ctttagtgat
    12661 tgataacaaa agaggtgcca ggaatgaaca aaacgctaat cgccgcagct gtggcaggga
    12721 tagttttact cgcttcaaac gctcaggcac aaaccgtacc ggaaggctat cagctacagc
    12781 aagtgctcat
//
LOCUS       DAJTMK010000117        12754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTMK010000117 DAJTMK010000000
VERSION     DAJTMK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.118"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(393..1181)
                     /gene="phoH"
                     /locus_tag="OO135_002840"
     CDS             complement(393..1181)
                     /gene="phoH"
                     /locus_tag="OO135_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5398623.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILVRNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1802..3073)
                     /gene="efeB"
                     /locus_tag="OO135_002841"
     CDS             complement(1802..3073)
                     /gene="efeB"
                     /locus_tag="OO135_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5398624.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3079..4206)
                     /gene="efeO"
                     /locus_tag="OO135_002842"
                     /pseudo
     CDS             complement(3079..4206)
                     /gene="efeO"
                     /locus_tag="OO135_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
     gene            complement(4264..5094)
                     /locus_tag="OO135_002843"
     CDS             complement(4264..5094)
                     /locus_tag="OO135_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5398625.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5763..7271)
                     /gene="putP"
                     /locus_tag="OO135_002844"
     CDS             complement(5763..7271)
                     /gene="putP"
                     /locus_tag="OO135_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR5398626.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(7430..7639)
                     /locus_tag="OO135_002845"
     CDS             complement(7430..7639)
                     /locus_tag="OO135_002845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398627.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7694..11656
                     /gene="putA"
                     /locus_tag="OO135_002846"
     CDS             7694..11656
                     /gene="putA"
                     /locus_tag="OO135_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5398628.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLTEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTID
                     "
     gene            complement(11696..12334)
                     /gene="rutR"
                     /locus_tag="OO135_002847"
     CDS             complement(11696..12334)
                     /gene="rutR"
                     /locus_tag="OO135_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5398629.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            12622..>12754
                     /locus_tag="OO135_002848"
     CDS             12622..>12754
                     /locus_tag="OO135_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5398630.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     "
BASE COUNT         3120 a         3507 c         3194 g         2933 t
ORIGIN      
        1 attaattaag attaacctta catttataat attatcccta aagaataaat cagaggaata
       61 tattcggtgt taaacagtca gtcaaagtat agcccattgt tgattaaacc tcataagcaa
      121 aattggcacc agatctcctg tacaggttga atggccgaaa aaagataggc agatgctcat
      181 ttttttggct tctggaagaa aaactctccg cgctggcagt attacactgg tctgatgcta
      241 tgacgaaaaa tgcctggtaa gagtatctaa accagactcc agttcagctt atcaacacgc
      301 tcaaagtcgc ccccctgaac gctaacctga agtgaaaatc tgttgaaata aaacaaagcc
      361 cggttcgccc gggctctgca ccgataacac acttaactgt atgcatgcag cgtacgttgg
      421 caaagtgccg aacgtacgca atcctctttt ccgaaccgga cgatcccaac catttcatct
      481 tcttcgaaac gttccagcgc gtcacttaat ccggagcaca cgccgcgagg caaatcgcat
      541 tgcgtgatat caccgttgac gataaccgtc acgttctccc cgaggcgggt taaaaacatt
      601 ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc attttcaaag
      661 gtacgtccac gcatataggc gaacggcgcg atttccacct tccctatttc cggtcgcagg
      721 cagtactgca taaaggaagc ccctaagcgc cggaccagca cgtcgtagac cgggcgaaaa
      781 tagggagcaa acttttctgc gatatcacca ggtaagaagc caagatcttc atcggcttgc
      841 agaactggac gggtgacgat aatcctgtcg acatccttat gtatcagggc ctctgccgct
      901 tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc ccgtggcgaa tatcagctgc
      961 ttactctcaa tagccttcag atagtgcaat tgcgcttcat ttcgcacgag gatgggcgag
     1021 gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc cgctcatctg cacaagcgag
     1081 gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag cacacgtttt
     1141 gcctcgcgac gagctttgat cactgctttt tgtcttccca tggagagcac cttgagttgt
     1201 ttgtattcat cacacgcgcc gttggcagcg tgattctgcg cacgaacatc agagggttgg
     1261 cttccttgta agccatagtt tgcttttgga taaaatgccg aaaaacggct acgcgcaccg
     1321 tttacggcgt cggtaacaca tgaaaagaaa ggatgaggtt gaaaatgcaa agtgacgaga
     1381 tgactaccgg aggagaaaac tccgcgagtg gtggcgcgtt gattatctaa aacatgtcca
     1441 gtacaggacg ttaccatccg cgatctccat agtgactgac tatcactgcc gggaacttcc
     1501 gctgctactt aataagtaca acagatctcg catttgttgc aacaatatat ttacttatat
     1561 ttaactataa aacactattt cagtgacatt agtttctact ggaaagatga cagagtgatg
     1621 acagtgatga aaaaagctgt gtgctttcag caggatttgc atcaggcatc agtgcacgat
     1681 cgccggatgc ggcgtgaacg ccttatccag cctaccaaac ccgcactgat tgtaggcatg
     1741 ataagacgcg caagcgtcgc atcaggcatc tgcgcacaaa tgccttatcc gcctaaaaac
     1801 attaaacccg caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg
     1861 aaaaaaaata accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc
     1921 ttttttgtac tgtcaggaag cctttttcca gatcgtgttg gtagcagaca aacagcaacc
     1981 ccatatccag ttgtccggag ttggtgaccc ccaacgaata actgtagccg cgacgcagca
     2041 tcaggctgga ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg
     2101 cgatcacctt cccttccggg tcgctggcgt aatcaggcac atcatgctca tgctgcattc
     2161 ccagcggcgc accggtttgc ttatcacggc caaaaatcgt ctgctgttct ttcagcggcg
     2221 ttctgtccca aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga
     2281 ttgtccacgc aggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat
     2341 cattctggct atcgggattg gcagtgccgt ctttaaagcc cagcaaatta atcggcgtct
     2401 ctttgccttt actacgcgct gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc
     2461 tgagcaaatc cggcgtgtgt ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg
     2521 tgttggcgca aatttgtagc aacacatcac catggcataa cgccgcatcc agcgagtcgt
     2581 tggggaaacg cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaaccgct
     2641 catcaaacaa tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc
     2701 cgccaagaat gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc
     2761 cgccctgagt cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg
     2821 ctttatcgct ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca
     2881 ggatccctgc ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa
     2941 gcgtacccgg cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg
     3001 ccagcgcacc tatcactttc agtaaacgtc ggcgtgacgg ttcattcacg ccgttttcat
     3061 ctttatactg cataacgctt aatccactcc cagcacaccg cgaagttgcg ccagatcttc
     3121 cgccagcgca gtaatcggtc ctttcagtgc attccggtcg gcatcagtca atttgtcgta
     3181 ggtttcaaaa ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt
     3241 ggcatcgact tttgccagca gttccgggtt ggctttttgc agttgtggac gcagcaaatc
     3301 gacaattttc tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta
     3361 gcgatcttct tcaccgctaa ttttgctggc tgccacttcc tcaatcagtc cggctgcgcc
     3421 gccgaccact tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac
     3481 atctgtataa agctgctcag cgtactgatc catccctttg gtggtgttgt cgccaaacaa
     3541 tgctttttcc agacggtgga aaccagtgaa ttttgggtcg gcggcttttt gctcgtaatc
     3601 atcttcacgg gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcaat
     3661 gcgctcatag tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgccagc
     3721 tttaatcgcg tcggtaaagg ctttggtatc ggtcaccagc tgcgtggttt ccgccatgac
     3781 atacgcttta tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc
     3841 atccgccgtt gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca
     3901 ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc
     3961 gatattttcc cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt
     4021 ctggctatgg ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg
     4081 ttagcactgc ttatccgtca cggtcacttt gacctgcggc acatcagcgg cgttagccat
     4141 aaaagcagaa gaaaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat
     4201 ggttatgacg agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat
     4261 ttgttacgcg gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag
     4321 cgccgggatg agataaataa accagacggc gacttcgctg acgctcggcg cttcctgata
     4381 gccaaaaatc ccttccatca gcgtgccaaa cagcgagtga gttgagagca ccgcactcat
     4441 atcgaaggcg atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc
     4501 accagctgcc agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc
     4561 accaagattg aggcgaatac cgccccagta gagcagaaag ccgagcacca cggctgtagc
     4621 aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag
     4681 caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag
     4741 cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac
     4801 tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc
     4861 gatcaccgcc acgatacctt caaacagttc ctgttctttt tgcggaaatt cgccggtggt
     4921 ttcgttaatg aagatcccca ggcccaggca caacgcagcg gcaagcaaca cgccaatcca
     4981 catcacacca atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac
     5041 aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc
     5101 cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg
     5161 attatcatta cggtaaagaa aaatacaagc ggaatgttta taaaatgatg ttggaatgta
     5221 aacaaagaga gataatgcca ggggatataa acgattattc cagggtagaa agatgggatt
     5281 gtttgcaaag aaccatgtgt tcttgccgga tgccgcgtaa acgccatatc cagccgacaa
     5341 aactgtccag actcaatgca tcagagcttc catgtaaaac tgataagcgg gtgtatcaag
     5401 caatttcgtc catccgcgat tatcaaatct aactttttgc ccggaacata ataacagctc
     5461 gggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccgggaca gcccacatgg
     5521 atgtgggctg agggcgcgtc ttacaggacg ttacctcgcg cccgacccgg tagccgaagg
     5581 ggataagctg aaggtaccgc gcagcggcga ttttgttcgc cagagcccgg gggtgcacag
     5641 ggggcggcgg cgattggccg ccccctgcgc gctccctgca ccattcaatt tatatggcga
     5701 ggcacattaa gggagcgcaa cccgctgctg aagccaaaca aaaccgccgc aggctaagtc
     5761 ccttagcttt cctgcaaccg tgacggcgga gccgaatgat agtgcgcatc ggcctcggca
     5821 aagcgttttt gcatcgccgc tgacggcgct ttacccagca aactaaacac tacaatcccg
     5881 atactgccga agatgaagcc cggaataatt tcgtacagtc ccagccagcc gaactgcttc
     5941 cagacgataa ccgtcagcgc accgatgatc atccccgcca gtgcaccgtt acgcgtcatg
     6001 cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg ccgcgccaaa gcctgcccac
     6061 gcgtagctca ctaagcccag cacgcggttt tccgggtttg ccgccagcgc aatcgccacc
     6121 agcgccacca ccagcaccat cacacgccct acccacacca gctctttctg gctggcatgt
     6181 ttacgcagaa acgctttgta taaatcttcg gtaatcgcac tggagcacac cagcagttgg
     6241 caacttaagg ttgacattac cgccgccaga attgccgaca gcagaatccc ggcaatccac
     6301 gggttaaaca gaatttgcgc cagttcgata aacacgcgct cggcgttctg gtttaccgcg
     6361 cctgctaccg ccgggtgctc attaaagtaa gcaatcccaa agaagccgac agccaccgcc
     6421 cctgccaggc agaggatcat ccaggtcata ctgatacgac gcgcatggac aatgctgtgg
     6481 tgagaatccg ccgccataaa acgcgccaga atgtgcggct gcccgaagta acccagcccc
     6541 caacccatca gtgagataat ggcaacaaag ttcagacctt tgagcatatc aacgttttcg
     6601 atgctctttt gtttgatcac ttccagcgag tcaccaaagc caccgacact gataatgacg
     6661 ataaccggtg tcaggatcag ggcaaaaatc atcaggctgg cctgtacagt gtcagtccag
     6721 ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg tcgccgcagc tcccgcccac
     6781 agagccgttt cgtagctcat gccaaaggta ctttcaaaca gacgcgcgcc tgccacaatg
     6841 cccgaagcgc aataaatggt gaagaacagc aaaataacca gcgcggagat aatgcgcaaa
     6901 atgcggcttt tatcttcaaa acgcccggtg aaataatccg gcaatgttaa ggcgttatta
     6961 ttgtattcgg tatgcacacg caaccgcccg gccaccagct tccagttgat ccacgcgcct
     7021 aatgtcaggc caatggcgat ccagctttcg gaaatcccgg aaagaaaaac cgcgccaggc
     7081 aaccccatta acagccagcc actcatatcc gacgcacccg ccgataatgc cgtcacaaat
     7141 ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt ttttcgttga tcgccaggcg
     7201 ataaacccaa tcaatatcat gccaaagata tagacacaaa atgtcaccaa catcggtgtg
     7261 ctaatagcca tctaaagtct ccaaaaaatt attatcggca atgtcgaaac ttgccgttat
     7321 atctgcaacc ggaacggggt aacagagttt attttttacc agggcgaccg tatcctgccg
     7381 gaagcgctgg ttattcacaa tcgatttaac acaccattta cattaaattt tagtgctcag
     7441 cgacactatt tttcatcagg ttgcactctc tcacattttt tgcggttgca cctttcaaaa
     7501 atgttaactg ccgcagagaa aaagtctgag ttattttttt aatccctgtc atatcaattt
     7561 cttttattaa catttcattc atttttaagc ttgctacgca ggtcacattt aacatggttg
     7621 cacaaagttg caacatcatg gatatttcac gataacgtta agttgcacct ttcagaacaa
     7681 caggagtaat ggcatgggaa ccaccaccat gggggttaag ctggacgacg cgacgcgtga
     7741 gcgtattaag tctgccgcga cacgtatcga tcgcacgcca cactggttaa ttaagcaggc
     7801 gattttttct tatctcgaac aactggaaaa cagcgatact ctgccagagc tacctgcgct
     7861 gctttctggt gctgccaatg agagcgatga agcaccgact ccggcagagg aaccacacca
     7921 gccattcctc gactttgccg agcaaatttt gccccagtcg gtttcccgcg ccgcgatcac
     7981 cgcggcctat cgtcgcccgg aaaccgaagc ggtttctatg ctgctggaac aagcccgcct
     8041 gccgcagcca gttgctgaac aggcgcataa actggcatat caactggccg ataaactgcg
     8101 taatcaaaaa aatgccagtg gtcgcgcagg tatggtccag gggttattgc aggagttttc
     8161 gctgtcatcg caggaaggcg tggcgctgat gtgtctgacg gaagcgttgt tgcgtattcc
     8221 cgacaaagcc acccgcgacg cgttaattcg cgacaaaatc agcaacggta actggcagtc
     8281 acacattggt cgtagcccgt cactgtttgt taatgccgct acctgggggc tgttgtttac
     8341 tggcaaactg gtttccaccc ataacgaagc cagcctctcc cgctcgctga accgcattat
     8401 cggtaaaagc ggtgaaccgc tgatccgcaa aggtgtggat atggcgatgc gcctgatggg
     8461 cgagcagttc gtcacgggtg aaaccatcgc ggaagcgtta gccaatgccc gcaagctgga
     8521 agagaaaggt ttccgttact cttacgatat gctgggcgaa gctgcactga ctgccgcaga
     8581 cgcacaggcg tatatggttt cctatcagca ggcgattcac gccattggta aagcgtctaa
     8641 cggtcgtggc atctatgaag gtccgggcat ttcaatcaag ctgtcggcgc tgcatccgcg
     8701 ctacagccgc gcccagtatg accgggtaat ggaagagctt tacccgcgtc tgaaatcact
     8761 caccctgctg gcgcgtcagt atgatattgg tattaacatc gatgccgaag aggccgatcg
     8821 tctggagatc tccctcgatc tgctggaaaa tctctgtttc gagccggaac tggcaggctg
     8881 gaacggcatc ggttttgtta ttcaggctta tcaaaaacgc tgcccgttgg tgatcgatta
     8941 cctggttgat ctcgccaccc gcagccgtcg ccgtctgatg attcgcctgg tgaaaggcgc
     9001 gtactgggat agtgaaatca agcgtgcgca gatggacggc cttgaaggct atcctgttta
     9061 tacccgcaag gtgtataccg acgtttctta tctcgcctgt gcgaaaaagc tgctggcggt
     9121 gccgaatcta atctacccgc agttcgcgac gcacaacgcc catacgctgg cagcgattta
     9181 tcaactggca ggtcagaact actatccggg tcagtacgag ttccagtgcc tgcatggtat
     9241 gggcgagcca ctgtacgagc aggtcacagg gaaagttgcc gacggtaaac ttaaccgacc
     9301 gtgtcgtatt tatgccccgg ttggcacaca tgaaacgctg ctggcgtatc tggtgcgtcg
     9361 cctgctggaa aacggtgcta acacctcgtt tgttaaccgt attgccgaca cctctttgcc
     9421 actggatgaa ctggtcgccg atccggtcac tgctgtagaa aaactggcgc aacaggaagg
     9481 gcaaactgga ttaccgcatc cgaaaattcc cctgccgcgc gatctttacg gtcacgggcg
     9541 cgacaactcg gcagggctgg atctcgctaa cgaacaccgt ctggcctcgc tctcctctgc
     9601 cctgctcaat agtgcactgc aaaaatggca ggccttgcca atgctggaac aaccggtagc
     9661 ggcaggtgag atgtcgcccg ttattaaccc tgcggaaccg aaagatattg tgggctttgt
     9721 gcgtgaagcc acgccgcgtg aagtagaaca ggcgctggaa agtgcggtta ataacgcgcc
     9781 aatctggttt gccacgcctc cggctgaacg cgcggcgatt ttgcatcgcg ctgccgtgct
     9841 aatggaaagc cagatgcagc aactgattgg tattctggtg cgtgaagccg gaaaaacctt
     9901 cagtaacgct attgccgaag tgcgcgaagc ggtcgatttt ctccactact acgccggaca
     9961 ggtgcgggat gatttcgcta acgaaaccca ccgtccatta gggcctgtgg tgtgtatcag
    10021 tccgtggaac ttcccgctgg ctattttcac cgggcagatc gctgccgcac tggcggcagg
    10081 taacagcgtg ctggcaaaac cggcagaaca aacgccgctg attgccgcgc aagggatcgc
    10141 cattttgctg gaagcgggtg taccccctgg cgtggtgcaa ttgctgccag gtcaggggga
    10201 aaccgtgggc gcgcaactga cgggtgatga tcgcgtgcgc ggggtgatgt ttaccggttc
    10261 aaccgaagtc gctacgttac tgcagtgcaa tatcgccagc cgcctggacg ctcagggtcg
    10321 ccctattccg ctcatcgctg aaaccggcgg catgaacgcg atgattgtcg attcttcagc
    10381 actgaccgaa caagtcgtcg tggatgtgct ggcctcggcg tttgacagtg cgggtcagcg
    10441 ttgttcggca ctgcgcgtgc tgtgcctgca agatgagatt gccgaccaca cgttgaaaat
    10501 gctgcgcggt gcaatggccg aatgccggat gggtaatccg ggtcgcctga ccaccgatat
    10561 cggtccggtg attgatagcg aagcgaaagc caatattgag cgccatattc agaccatgcg
    10621 tagcaaaggc cgtccggtgt tccaggcggt gcgggaaaac agcgaagatg cccgtgaatg
    10681 gcaaagcggc acctttgtcg ccccgacgct gatcgaactg gatgactttg ccgaattgca
    10741 caaagaggtc tttggtccgg tgctgcatgt ggtgcgttac aaccgtaacc agctaccaga
    10801 gctgatcgag cagattaacg cttccggcta tggtctgacg cttggtgtcc atacgcgtat
    10861 tgatgaaacc atcgcccagg tcactggctc ggcccatgtt ggtaacctgt atgttaaccg
    10921 taatatggtg ggcgcagtgg ttggtgtgca gccgttcggc ggcgaagggt tgtccggtac
    10981 cgggccgaaa gcaggcggtc cgctctatct ctaccgtctg ctggcgaatc gcccggaaag
    11041 tgcgctggca gtgacgctcg cgcgtcagga tgcagagtat ccggtcgatg cacagttgaa
    11101 agccgcattg actcagccgc taaatgcact gcgggaatgg gcagcaaatc gtccagaatt
    11161 gcaggcgtta tgtacgcaat atggcgagct ggcgcaggca ggaatacaac gattgctgcc
    11221 ggggccgacg ggtgaacgca acacctggac gctgctgccg cgtgagcgcg tgttgtgtat
    11281 tgccgatgat gagcaggatg cgctgactca gctcgccgcc gtgctggcgg tgggcagcca
    11341 ggtactgtgg ccggatgacg cgctgcatcg tcagctagta aaggcattgc catcggcagt
    11401 cagcgaacgt attcaactgg cgaaagcgga aaatataacc gctcaaccct ttgatgcggt
    11461 gatcttccac ggtgattcgg atcagcttcg cgcattgtgt gaagccgttg ctgcgcggga
    11521 tggcgcaatt gtttcggtgc agggttttgc ccgtggcgaa agcaatatcc ttctggaacg
    11581 gctgtatatc gaacgttcgc tgagtgtgaa taccgctgcc gctggcggta acgccagttt
    11641 aatgacgatc gattaaacag gtagccggag gatgttacac cctcctccgg catctttaac
    11701 gtggtcgaat cccctcaata ataatccgct gcacgttttc aaccgtttga ttgaaaaata
    11761 cctcatcgcg caacgttgcg cctgtcaccg cctccacctg aggggcgaaa tcggcgtaat
    11821 gttgagtgga agcccaaatc ataaaaatca aatgctgcgg atcaatcggc gcgagtttgc
    11881 cgcttttgac ccaaccggca atcagcgccg atttctcatc aattaatgcc ttcaaatcgc
    11941 ccgtcagttc atccattaac agcggcgcgc ctgccagcat ctccatacag aacaggcgcg
    12001 aagcctgcgg ataatcgcgt gagacttcca gcttcagacg gatgtactct ttgatcgccg
    12061 ccagcggggc gaaatcttca cgaaacgctt ttaacggtgc cagccagata tcgagaatct
    12121 gccgcagcac ggcaatatac agcgcctctt ttgacggaaa gtaatacagc agattggttt
    12181 ttgaaacacc cgccaactct gcgatctgct ccagccttgt gccgtgaaaa ccgaattgtg
    12241 aaaaagtgtc cagtgctgcg ctaagaatcg cttttttctt cgcgcttact gcgcgcgaac
    12301 gtttacccgt tgttttcact gcgccttgcg tcatgcgctc tcccctcttg tagattggca
    12361 acaggatagc aaaacccccg cccggcctcg accttctgca ctctcatcgc gcacggatgc
    12421 atgttttatg tgcaactgtt ttgaccgttt agtccacttt ttaccagata ttttagataa
    12481 cacaatgcta acacattaat aacattcact ttttaaaaac tggcatccgc tttgcaaaca
    12541 agccaatacg cggctgaaaa ggatatgcag gatgcagcgc cccgtctgac tttcacttta
    12601 cgagacgaag agaggttgat gatgaaaatt ggcgtattcg tacctattgg caacaacggc
    12661 tggctcattt cgacccacgc gccgcagtac atgccgacct ttgaactgaa taaagccatt
    12721 gtgcaaaaag cggagcatta ccatttcgat ttcg
//
LOCUS       DAJTMK010000118        12662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTMK010000118 DAJTMK010000000
VERSION     DAJTMK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.119"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(17..93)
                     /locus_tag="OO135_002849"
     tRNA            complement(17..93)
                     /locus_tag="OO135_002849"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(57..59),aa:Met,seq:cat)
     gene            302..1399
                     /gene="mltA"
                     /locus_tag="OO135_002850"
     CDS             302..1399
                     /gene="mltA"
                     /locus_tag="OO135_002850"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5398631.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1476..2282
                     /gene="tcdA"
                     /locus_tag="OO135_002851"
     CDS             1476..2282
                     /gene="tcdA"
                     /locus_tag="OO135_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5398632.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2333..2776)
                     /gene="csdE"
                     /locus_tag="OO135_002852"
     CDS             complement(2333..2776)
                     /gene="csdE"
                     /locus_tag="OO135_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5398633.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2776..3981)
                     /gene="csdA"
                     /locus_tag="OO135_002853"
     CDS             complement(2776..3981)
                     /gene="csdA"
                     /locus_tag="OO135_002853"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5398634.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKIIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4173..4400
                     /locus_tag="OO135_002854"
     CDS             4173..4400
                     /locus_tag="OO135_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5398635.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4751..5668
                     /gene="gcvA"
                     /locus_tag="OO135_002855"
     CDS             4751..5668
                     /gene="gcvA"
                     /locus_tag="OO135_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5398636.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5687..6082
                     /locus_tag="OO135_002856"
     CDS             5687..6082
                     /locus_tag="OO135_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5398637.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKLKGVSHE"
     gene            6075..7175
                     /gene="rlmM"
                     /locus_tag="OO135_002857"
     CDS             6075..7175
                     /gene="rlmM"
                     /locus_tag="OO135_002857"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5398638.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7219..7950)
                     /gene="fucR"
                     /locus_tag="OO135_002858"
     CDS             complement(7219..7950)
                     /gene="fucR"
                     /locus_tag="OO135_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5398639.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8008..8430)
                     /gene="fucU"
                     /locus_tag="OO135_002859"
                     /pseudo
     CDS             complement(8008..8430)
                     /gene="fucU"
                     /locus_tag="OO135_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
     gene            complement(8432..9850)
                     /gene="fucK"
                     /locus_tag="OO135_002860"
     CDS             complement(8432..9850)
                     /gene="fucK"
                     /locus_tag="OO135_002860"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR5398640.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLKRAHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9847..11592)
                     /gene="fucI"
                     /locus_tag="OO135_002861"
     CDS             complement(9847..11592)
                     /gene="fucI"
                     /locus_tag="OO135_002861"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5398641.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(11625..>12662)
                     /gene="fucP"
                     /locus_tag="OO135_002862"
     CDS             complement(11625..>12662)
                     /gene="fucP"
                     /locus_tag="OO135_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5398642.1"
                     /translation="IITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETA
                     ANPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPE
                     QLSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSR
                     LARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIG
                     RFTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIF
                     SLGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFTVIFI
                     FARFRSQTATN"
BASE COUNT         3027 a         3167 c         3355 g         3113 t
ORIGIN      
        1 ttcatcaaat tttaattggt tgcgggggcc ggatttgaac cgacgacctt cgggttatga
       61 gcccgacgag ctaccaggct gctccacccc gcgtccgtgg atgcgcacta tactccgctc
      121 gcgttttcat gcaacctttt ttttaccccg atcacggatt tagcatgatt ttgaacaaaa
      181 acaaaccact aaagcctatt ttttgtgcaa aatttgcgtc agggagaggc tgctcatttc
      241 attaaacgcg gcggtttgtt atcttcgttg cgccttattt tttaacctga agaagagaac
      301 aatgaaagga cgttgggtaa agtaccttct tatgggcacg gttgtggcaa tgcttgccgc
      361 ctgctcttcc aaatcaaccg atcgcggaca gcaatataaa gacgggaaat ttacccagcc
      421 tttctctctg gtgaaccagc cagatgccgt tggcgcgccg attaacgccg gtgattttgc
      481 cgagcaaatt aaccatatcc gtaattcgtc accgcgtctg tatggcaacc agagtaatgt
      541 ttataacgcg gtgcaagagt ggctgcgcgc aggcggtgat acccgcaata tgcgccagtt
      601 cggcattgat gcctggcaga tggaaggtgc cgacaactat ggtaacgtgc agtttacagg
      661 ctattacacg ccggtaattc aggcgcgcca tacccgccag ggcgagttcc agtatcctat
      721 ttaccgtatg ccgccaaaac gtggtcgtct gccgtctcgt gcggagatct acgcgggggc
      781 attgggcgat aaatacatcc tcgcttacag caactctctg atggataact tcattatgga
      841 tgtgcagggt agtgggtata tcgactttgg tgatggcagt ccacttaact ttttcagcta
      901 tgcagggaaa aacggtcatg cctatcgcag cattggtaag gtgctgatcg accgtggcga
      961 agtgaaaaaa gaagatatgt cgatgcaggc gattcgtcat tggggcgaaa cacacagtga
     1021 agccgaggtt cgcgagctgc tggaacagaa cccgtctttc gtcttcttta aaccgcaatc
     1081 ttttgcaccg gtgaaagggg caagtgcggt gccgctggtt ggccgtgctt ctgttgcctc
     1141 cgatcgttca attattccgc caggtactac cttgctggca gaagtgccgc tgctggataa
     1201 taacggcaaa tttaacggtc agtacgaatt gcgtctgatg gtggcgctgg atgtcggtgg
     1261 cgcaatcaaa ggccaacact tcgatatcta tcaagggatc gggccggaag ccggacatcg
     1321 cgcaggttgg tacaaccact atggacgtgt ctgggtgctg aaaaccgccc cgggcgcagg
     1381 taacgtcttt agcggctgat gtgttattct gtataccgtt tttacggata cgacagggtg
     1441 agggataacc tcaccctttt taattctgag gtgctatgtc tgtggtaatt agtgatgcat
     1501 ggcgtcagcg ttttggtggc acagcgcgtc tgtatggtga aaaagcgttg caactgtttg
     1561 ctgacgcgca tatttgtgtg gttggtatcg gtggtgtcgg ttcctgggcg gcggaagcgc
     1621 tggcgcgcac ggggattggc gcaatcacgc ttatcgatat ggatgatgtg tgcgtcacca
     1681 ataccaatcg gcaaattcac gctctgcgcg ataacgtcgg gctggcaaaa gcggaagtca
     1741 tggcggagcg cattcgccag attaacccgg agtgccgtgt aacggtggtg gatgatttcg
     1801 tgacgccgga taacgtagcg caatatatga gtgccggtta ttcgtacgtg attgatgcca
     1861 ttgatagtgt acgacccaaa gcggcgctga ttgcttattg tcggcgcaat aaaatcccgc
     1921 tggtcacgac cggtggcgcg ggtgggcaga ttgacccgac gcagattcag gttaccgatc
     1981 tggcgaaaac gattcaggac ccgctggcgg cgaagttgcg cgagcgcctg aaaagcgatt
     2041 ttggcgtagt gaaaaacagt ataggtaagc tcggcgtgga ttgcgtgttt tctactgaag
     2101 cgctggtgta cccgcagtca gacggtacgg tgtgtgcgat gaaagccacg gcagaagggc
     2161 cgaagcggat ggattgtgca tctggatttg gcgcggcaac gatggtgacc gccacctttg
     2221 ggtttgttgc ggtttctcat gcgctgaaga agatgatggc gaaagcagcg cgtcagggtt
     2281 aaacaatcag gtcggatgcg acgcgaacgt cttatccgac ctggttctgt gcttaaacct
     2341 gcttcgccgc agcgataatt gcctcgctta acgcattcaa cccctggctg cgtgaggcgc
     2401 taagctgccc acgcaatccc agctcatcaa acaatgccag cggcgactgt gcctgtaatt
     2461 cggcggcggt tttcccctca acggcagtca acaacaccgc caacaggccg cgcacaatgc
     2521 gcccttcgct gtcgccaaag aagtgcattt taccgttttc agccacggta tatcccagcc
     2581 agacgcggtt ttcgcatccg gcaatctctt tagcctgcgc ttttaactcg tctggcaaag
     2641 ccggaagctg tttccccagc atgatcaact ggcgatattt atcttcccat tgcgtcaacg
     2701 gtgcgaaggt attgcgtaac gtttctgcgg ttacggttgt gccgaacgga tgtccggcga
     2761 attgcgggtt tgtcattaat ccaccaataa ttccagcgcg cggtcaacgg cattcaccag
     2821 cgcatccaca tcactctttg tattatatgg cgcaaaagag gcgcgcagtg tgccggttac
     2881 gcctaattct gccagtagcg gctgagcgca atgttgcccg gcccgcaagg caataccgta
     2941 ctccgccagt agcgtcacca tatcgctgtg atgaacgcca gcaaaatcaa aggccagcag
     3001 gctggaatcc tggcagcgga atgaacgaaa gcctggacgt ttcgccagcg catcttccgc
     3061 cagcgttgct aagctatggc tccagttttc ggcctggttg atatcgtaat ctgccagcca
     3121 ttccagcgcc gcgcttaatc ctatgacacc agccacattt ggcgttcctg cttccagttt
     3181 ccacggcgca gattgagtcg tgaagccgtc aaaactcact tcgtgaatta ttttgccgcc
     3241 acccagccag ggcgacatcg cttccagcag ttctgatttg ccatacagca cgccgatacc
     3301 cgtcgggcca tacagtttgt gaccagagaa agcatagaaa tcgatatcca gttgctgaac
     3361 atccgcgggg aaatgcaccg ccccctgagc accatcaacc atcaccacca tccctgctga
     3421 atgagcaaag gtaatcgctc gcgccagatc cgggcaaccg ccagtaacgt tcgacatctg
     3481 actcaacgcc agaatccgac tacggggagt aatcagttct ggcaacaaat cgacatccgg
     3541 cagtcgctgc gcattaagcg gcaatttcac cactttggct ccagtttgtt gggcgaccat
     3601 cagccagggg acgaggttgg cgtggtgttc tgccacgctg acaataatct catcgcccgg
     3661 ttgcagacgc ggacgcgcat agcattgtgc caccatgttg atggattcag tggtgccgcg
     3721 cgtccagacg atagttttat catccggtgc attcagtaat tgcgccactt tctctcgtgc
     3781 agcttcataa cgcgcggtca ggcgttgggc ttcggcaaac tggctgcgat ggacgtttcc
     3841 ggcgctcagg ctgtaaaact gtcgagtggc ttcaaccacg gcttcaggtt taagcgcggt
     3901 cgcggcgctg tcgagataga cgcccgcatc ctgtagtgcg ggaaactggg cgcgaaactg
     3961 cgcgggatta aaaacgttca tggtactcct cggcttgatg caccgatcgt gacgcaaaat
     4021 tcactttgat acaaggagag tgctaaccat cagaattatc tggatatcct ggcgaagccg
     4081 cgtaagtaac atatgctgaa tagtgttagg cgttaatttt agcctgttaa tcaatactat
     4141 taagatttat taatcgcttt aaggagaaga tgatgaaaaa gactgccgca attatttctg
     4201 cctgtatgct gacttttgcc ctgagcgcct gttccggttc gaactatgtg atgcacacca
     4261 atgacggacg taccatcgtc tctgacggca aaccacagac tgataacgat accggtatga
     4321 tttcgtataa agacgctaat ggcaacaaac agcagatcaa ccgtactgac gtgaaagaga
     4381 tggtcgaact ggatcagtaa ttcgcgatcg caaggtaaaa aaaagcaccg caattaggcg
     4441 gtgctacatt aatcactatg gacagacagg gtaaatgtac aggaagtgaa aaaaggtagc
     4501 tttgctacca tggtctgaat cgcagaccaa ttgcaaacac aacaacacaa catcacaacc
     4561 gtaagccaaa agttcaccag aacacgcatt ccgataaaac ttttcgttcc ggctcaggaa
     4621 gtgccgccac tataggtatt tgctggtaga agctcaacgg ataatttata atggctcaga
     4681 ttaaaaaaac taataggtca catagtgtga tctaattgtt aaattcattt aacatcaaag
     4741 tttaatagcc atgtctaaac gattaccacc gctaaatgcc ttacgagttt ttgatgccgc
     4801 agcacgccat ttaagtttca ctcgcgcagc agaagagctt tttgtgaccc aggccgcagt
     4861 aagtcatcaa atcaagtctc ttgaggattt tttggggcta aaactgttcc gccgccgtaa
     4921 tcgttcactc ctgctgaccg aggaagggca aagctatttc ctcgatatca aagagatatt
     4981 ttcgcaatta accgaagcga cgcgtaaact ccaggcccgt agcgccaagg gggcgttgac
     5041 ggtcagttta ctccccagtt tcgccattca ttggttggtt ccgcgacttt ccagctttaa
     5101 ttcagcttat ccgggaattg acgttcgaat ccaggcggtt gatcgtcagg aagataagct
     5161 ggcggatgat gttgatgtgg cgatatttta tggtcggggc aactggccgg ggctacgggt
     5221 ggaaaaactg tacgccgaat atttattgcc ggtgtgttcg ccgctactgc tgactggcga
     5281 aaaacccttg aagacaccgg aagatctggc taaacatacg ttattacatg atgcttcgcg
     5341 ccgtgactgg cagacatata cccgacagtt ggggttaaat catatcaacg ttcagcaagg
     5401 gccaattttt agccatagcg ccatggtgct gcaagcggct atccacgggc agggagtggc
     5461 actggcaaat aacgtgatgg cgcaatctga aatcgaggcc ggacgtcttg tttgcccgtt
     5521 taatgatgtt ctggtcagta aaaacgcttt ttatctggtt tgtcatgaca gccaggcaga
     5581 actgggtaaa atagccgcct ttcgccaatg gatcctggcg aaagccgctg ctgaacaaga
     5641 aaaattccgc tttcgttatg aacaataatt tacgtagggt acgaccatga ccagccgttt
     5701 tatgctgatt ttcgccgcca ttagcggctt catttttgtg gctctgggcg cttttggcgc
     5761 gcatgtgtta agtaaaacca tgggggccgt tgagatgggc tggatccaga ccggcctcga
     5821 ataccaggcg tttcatacgc tggcgatctt aggtctggcg gtggcaatgc agcgtcgcat
     5881 cagtatctgg ttttactgga gtagcgtttt cctcgcgtta ggcacggtgt tgttcagcgg
     5941 cagcctttat tgcctggcgc tgtcccatct gcgtttgtgg gcgtttgtca ctccggttgg
     6001 cggcgtgagc ttcctcgcgg gctgggcgtt aatgttagtt ggtgctatcc gtttaaagct
     6061 caagggcgta agtcatgaat aaggttgtat tgctgtgccg tccgggcttt gaaaaagagt
     6121 gcgccgcaga aattaccgat aaagccggcc agcgggaaat tttcggtttt gcccgcgtga
     6181 aagagaatgc gggttatgtc atttatgaat gttatcaacc tgatgatggc gataagttaa
     6241 tccgtgagct gccgttcagt tcattaattt ttgcccgcca gtggtttgtg gtgggggaac
     6301 tcctgcagca tttgccgcca gaagatcgta ttacccccat tgtcggcatg ttacagggcg
     6361 tagtagagaa gggcggtgaa ctgcgtgttg aagttgccga taccaacgaa agcaaagagt
     6421 tgctgaaatt ctgccgtaaa tttactgttc cgctacgcgc tgccttgcgc gatgccgggg
     6481 tgctggcgaa ctatgaaacg ccgaagcgtc cggttgtgca tgtattcttt attgcaccag
     6541 gctgctgcta taccggttac tcatacagca acaataattc gccgttctat atgggcattc
     6601 cgcgcctgaa atttccggca gaggcgccga gtcgttccac gctcaaactg gaagaggcat
     6661 ttcatgtgtt tattcctgcg gatgagtggg atgaacgcct ggcgaacggg atgtgggcgg
     6721 tggatttagg cgcttgccct ggcggctgga cctaccaact ggtgaagcgc aacatgtggg
     6781 tttattccgt cgacaacggc ccgatggcgc aaagtctgat ggataccggg caggtgacgt
     6841 ggctgcggga agacggtttc aaattccgtc cgacacgcag caatatctcc tggatggtat
     6901 gcgatatggt tgaaaaaccg gctaaagttg cggcattgat ggcgcagtgg ctggttaatg
     6961 gctggtgccg tgaaaccatt ttcaatctca aactgccgat gaaaaaacgc tacgaagaag
     7021 tatcacacaa tctggcgtat attcaggcac agcttgatga acatggcata aatgctcaga
     7081 ttcaggcgcg gcagttgtat cacgatcgcg aagaagtgac ggtgcacgtc cgccgcatct
     7141 gggctgcggt gggtggtcgt cgcgacgagc gataacaggc aaggccgggg cgtcgtcttc
     7201 ggcctgctac cctttccgtc aggctgttac caaagaagtt gcaacctggc gctcatcaga
     7261 aataatgtgc gttacctcat ccagatgccc gatgcgggct tcccctgaac gattaaattt
     7321 acttttatca atcaataaca acgattgcgc ggcacgtttt aatagcatcg atttgtaatc
     7381 agcgttgatc gcattggagt cccacagtgc gccgctgcta tcgatccctt cacaagaaaa
     7441 aataaacaga tcgatttcca gcgatttaag ttgggaaatc agcgagggat tgacgtaaca
     7501 gccatatttg cgctcaagtg tgccgccgga actgatcagt tgaatgcgtt cgcgtttacc
     7561 gagttcatgg caaatcggat ggctattggt gaagacctga atgttgatgt caggcaactg
     7621 gcgtgccaga taccagcaag ttgaactggc gtctaaggct atcaccatcc cttcttcaat
     7681 ccacgcgagc gcctcgcgcg cgatatctgc tttatgcgca taatggcttt tcagcctgat
     7741 gtgaaaggga tcgccactgt cttgattttg acggtggata tatttagcgc gtccatgatt
     7801 gcgcagaatt ttaccctgcg tctgtaattc attgagatcg cgacgaatgg tttctttact
     7861 gacctttagc tgttcagaga gagcttccgt ggtcaggctg gtatggttca gcagcaggtc
     7921 gactatcgct tgctggcgtg ccgctttcat atttttcacc ccatcattcg ataaccgccc
     7981 gcgtcatgcg ggcgtaccgg tatgagatta cggtgttacc ccttttttta aaagaatatt
     8041 cccgtacttc gctcgttcgc ctgtgataac gatcgcaaag gctttttgcg cccgttcata
     8101 aaacgcaaaa cgattgatgc ggatgatgtc aggacacggg gcttgtagtg aaagcgcatt
     8161 acggtaacgt cgttctactt caggatcgag agtgtcacct tctaccgccg ccatcatcac
     8221 cagcggcggt gcataactgt ccagttcaaa taacgggata atcgcctgga gcaagtcgct
     8281 caccaacagg ccatcagcgc ggatcacctg cggtcccatc gaatgggcgg gaaagtgagc
     8341 atcggaaaaa ataatttaat ctccatgtcc catctctgcc agcactttca atagttcggg
     8401 agaaattaac ggcgaaattg ttttcagcat ttcacacttc ctctataaat tcaggttcag
     8461 tttgcgggta gaaataacgg tactgataat gaatctgtgc gcgggcttct tcagggctgt
     8521 taaattcccc tacgccatac caaccgaaca gcgcagctcc tgcgacggtc gtttcggcgt
     8581 cgtcgagaac ttttaccgga atatcaagca tattggcttt aatctgattc cacaatgtgt
     8641 tgcgacttcc tccaccgact aacaataatt cagaggcctt aaagtgcccg attttttcca
     8701 gcatctgtag attgcgctgt aattgcgcag ttaacccttc cagcgccgcg cgatagaaat
     8761 gcccccgcgt ggtattaagc gtcactcctt gccagccagc gttctgacac gacaataaat
     8821 cacactgcat ttttacgcca tccgcgctag gcgcgatcag acgagcttct tcaatcaaca
     8881 tttgccaggg tgtttcagcc gtccagaaca gttttctcac ccattccagc acgccggatg
     8941 ccagccattg catacctggg ttatacaacc ctgcctggct atccagttcg caggtggaac
     9001 cggcgtactg acttaacagc gaagtatcaa cctgggcgct acggaccatt aaaatttccc
     9061 atgtaccgga agagagcacg ggttcatttt ggtcggcacc agcgccaaaa agggcgaact
     9121 gggtatcgtg acctgcggaa atcaccggta tgccaacggg taagccgagc attgctgcgg
     9181 cgctgttctg tagcgtacca atctgttcac ccgcttccac cagacgaggg aagagtcggc
     9241 gtggaatacc agtggcttgt aaaatttgcg gactgaaatc gcgttgctgg atatccagca
     9301 tctggctggt tccggccatc gtgatatcag tagtgaattc gccggttaaa cggtggttaa
     9361 tcagcgacga aataaagaac caggcgtgcg cgcgtttcag cagttgtgga tgattttctt
     9421 tcaaccacac caacttatat aacgtattga aactaaaggc tccgacgcca gaaatagcct
     9481 gcaaccgctg tgcggagatc aactgttcaa tattgtccat aaccgctgct gttcgcggac
     9541 atttccagct aatgatcgga tagagcagat tgccttgctt atctaccaga gcgccatcca
     9601 caccaaaggt ggtgacggcg ataccgcgga tgtggcattc agtcagttca ctattgattt
     9661 gccgacagca atcagcaaag cgttgcaaaa tggcgtctaa agaccactgg tgccaggtgt
     9721 tgttttccat cgcgatatcg ctggcattag gcgttgaggc gcgggcaaca atttttccct
     9781 gccgattaac cgcgatggcc ctgacattgg tcgcgccaca gtcgagtacc aggataactt
     9841 cttgtttcat aatcgctcct gcaatacttc aatatccatg ccgtgcgcag cccaggcaga
     9901 aggacgatac actttggtct cttcaacgtt gtgcatacat accgggatac gcagcatgga
     9961 ggcgagagtg ataaagtcag cgccaacgtg gccgatggtc agaaccccat ggttagcgcc
    10021 ccagttcgcc attaccgagt acacatccgt aaacggccct ttaccggtga ggcgcggtgc
    10081 aaaccaggtg gttggccagg ttgagttggt gcgtttgttg aggatgtcat gcacatcctt
    10141 cggcaattcc acgctccagc cttccgcgat ttgcagtacc ggtcccaggc ctttgatgat
    10201 gttgacacga gtcatggtga acgggacgcc gccttcggta aggaagcggg aagagtaacc
    10261 gccgccgcgg aagtattcgt ggatcgccgg gcaccattcg gtagcggcga ggcaagcgtc
    10321 agcctcttgc tgagagattt cccagtgcgg cttcatcgtc gggttacctt cgctgtcgcg
    10381 ttgtttacag gaaccgtcca gcgcagcaga acctgagttg atcaaatgga tgatgccgtg
    10441 ttctgccagt ccatcgagtt tatgccccgt tacacgctca attgcttctg gtgaccagta
    10501 ggtacgcaca tcggcaaata cctgagcggt gccggtgagc tggtgaccca ttagcattgc
    10561 cacgccgtta agactgtcgt tttcggtcgc cacgacaaag ggttcgcgca cgccattcca
    10621 gtcaaatgaa ctgttgagga tcgcttcggc ggtgtcacca ttgggatatt gatcggtcca
    10681 gtgacgttgc ccctggaagc ccgcagcgat ggcgttgtag ccaagtgatt cttccacgcg
    10741 accaatatcg gccagtttgc tgttgccttg catcatgtcg cggatacaca tcgccatcag
    10801 taaactttcg cgcagaactg cgcggctttg ctcggcattg cgttgatact gtttgttatt
    10861 ttcatcttcg ccatagcgga agtttttatc agcccaggcc agtgccattt ccagttcggc
    10921 ttcgtcgtaa atcttctgat cgatacggcg acgcagttca gtcatatcca ccgcctggac
    10981 tttcattccc agccaggatt cgaagaagtt gtgatcaacg atggaaccgg cgatacccat
    11041 cgaaacgccg cccagcgaca gatagctttt acctttcatg ctggcgacgg ccaaaccggc
    11101 gcgggcaaag cgcagcagtt tttcttcaac atcggcagga atcgatgtgt catcggcatc
    11161 ctgaacgtca tgaccgtaaa tggagaatgc cgggatgcct ttctggctgt gagctgccag
    11221 agccgctgcc aggtaaacag cgccgggacg ttcagtgccg ttaaagcccc aaatggcttt
    11281 cgggcgggtt ggatccatgt cgatggtttc actgccatag caccagcaag gcgttaccgt
    11341 aatggtgagg cctacattct ggctgctgaa tttttcttcg caagcagcgg cttcagccat
    11401 acccgcgata caggtatcgg aaatgacaca ctcgacggca gctccgcagg catggcgcag
    11461 tttctcggtc agcagtgcgg ccgtagcttt cgccatattc attgtttgtt cttcaagcga
    11521 ctcacgaaca cccatgcgac gaccgtcaat aaccgggcgg ataccaattt tcggtaagct
    11581 gatttttttc attacagatt cctcacttta ttcggaaaat atgttcagtt agttgccgtt
    11641 tgagagcgga aacgggcaaa gataaagatg accgtgaagc agagtgcggg gatcagttca
    11701 gcagtgggga tgttgcccgc cgcgtcactg acaaaaccca tgaccggagt gacaataccg
    11761 ccgccaataa tggtcataac gatgaaggac gaaccgtatt tggtgtcctg gccgagattc
    11821 ttaatgccca gcgagaagat tgttgggtac tgaatcgaca taaaggcgct gcataaagtc
    11881 agggctatta agcccacatg accgccagcg aaggctgaga tcaggcacag tgccatagcg
    11941 attaatgcgt aggcggccag gactttgtgt ggtgcgaagc gactgatgag ccaggtaccg
    12001 gtgaaacgac caataaagaa gcacaccatg gttccggtta aatagttagc ggcaaagcct
    12061 gcagtcatac ctggaatttc ttctacagcg tagcgaatca aatagctcca gcaggccgtt
    12121 tgtgcgccaa cgtagcagaa ttgcgctaat actgcccagc gccagtggcg aatacgcgcc
    12181 aggcgagaaa gcgatgcgga gaacgatcct tgtttggcgt cactgtgatt atcactctgc
    12241 aatgccggga atttcgtcag catgatcagc agggcgacca gtaacacgat agccacgatg
    12301 atcatataag gtgtctgtac cgataatacc aggctgtgtt tatacgcact caattgctct
    12361 ggagacattt tatcgagaac gtcttgcgat tgatgtggca cgttagacaa aataagactt
    12421 tgcccaaaga caaccgcgat aattgcgcca aacgagttaa atgtttgcgc aagatttaag
    12481 cggaagtgac cactgctttc cggccctaat accgtaacaa aagggtttgc ggcagtttcc
    12541 agacaaccta atccggctgc aataataaat aggccaacta aaaataaggt gtagttcatt
    12601 atttctgcag cgggccagaa taatgcagcg cccagggcat ataaaaataa cccagtaata
    12661 at
//
LOCUS       DAJTMK010000119        12645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTMK010000119 DAJTMK010000000
VERSION     DAJTMK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.120"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(5..325)
                     /gene="chbB"
                     /locus_tag="OO135_002863"
     CDS             complement(5..325)
                     /gene="chbB"
                     /locus_tag="OO135_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5398643.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(623..961)
                     /gene="osmE"
                     /locus_tag="OO135_002864"
     CDS             complement(623..961)
                     /gene="osmE"
                     /locus_tag="OO135_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5398644.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1163..1990
                     /gene="nadE"
                     /locus_tag="OO135_002865"
     CDS             1163..1990
                     /gene="nadE"
                     /locus_tag="OO135_002865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5398645.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2220..3107
                     /gene="cho"
                     /locus_tag="OO135_002866"
     CDS             2220..3107
                     /gene="cho"
                     /locus_tag="OO135_002866"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5398646.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3067..3642)
                     /gene="ves"
                     /locus_tag="OO135_002867"
     CDS             complement(3067..3642)
                     /gene="ves"
                     /locus_tag="OO135_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5398647.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3845..4315)
                     /gene="spy"
                     /locus_tag="OO135_002868"
     CDS             complement(3845..4315)
                     /gene="spy"
                     /locus_tag="OO135_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5398648.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4645..5613)
                     /gene="astE"
                     /locus_tag="OO135_002869"
     CDS             complement(4645..5613)
                     /gene="astE"
                     /locus_tag="OO135_002869"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5398649.1"
                     /translation="MDNFLALTLTGKKTVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5606..6949)
                     /gene="astB"
                     /locus_tag="OO135_002870"
     CDS             complement(5606..6949)
                     /gene="astB"
                     /locus_tag="OO135_002870"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5398650.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVTRLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6946..8408)
                     /gene="astD"
                     /locus_tag="OO135_002871"
                     /pseudo
     CDS             complement(6946..8408)
                     /gene="astD"
                     /locus_tag="OO135_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(8405..9439)
                     /gene="astA"
                     /locus_tag="OO135_002872"
     CDS             complement(8405..9439)
                     /gene="astA"
                     /locus_tag="OO135_002872"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5398651.1"
                     /translation="MMVIRPVERSDVSTLMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9436..10656)
                     /gene="astC"
                     /locus_tag="OO135_002873"
     CDS             complement(9436..10656)
                     /gene="astC"
                     /locus_tag="OO135_002873"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR5398652.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11102..11908
                     /gene="xthA"
                     /locus_tag="OO135_002874"
     CDS             11102..11908
                     /gene="xthA"
                     /locus_tag="OO135_002874"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5398653.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12075..>12645
                     /locus_tag="OO135_002875"
     CDS             12075..>12645
                     /locus_tag="OO135_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5398654.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         3071 a         3419 c         3098 g         3057 t
ORIGIN      
        1 taaattaatt tgctgcggct tttttaatcg ctgcaaccgc agccttaagc acgcctaaac
       61 catcgacttt gccataaagc agcgagtcaa ttacttcaac cggtttgttg ggtaacaaac
      121 gctggatttc gggcaacata taagcaatct gcggccctaa taacacgaca tcggcattct
      181 gacctttttc accagccagt gtttccggaa atgcttcaat aatgaccgga acttcatatt
      241 tttctgcctg tgcgcgcatt tttgatacca gtaaagaggt agacatgccc gcagaacaaa
      301 acagataaat gtgtttcttt tccataaaac tgccctcatc gacgattatc tgtcagccag
      361 acactccgca agccttaacc tgcttccatg ctctgggtaa cttgcgaaac caacatgact
      421 ttttttgtgg ctgaaaggag tatacggtat agacctgagc aatagtattt ctttgacctt
      481 ataaattgtc tctccttgac ctgcggttat gaccctcttc acatttcggg caaataattc
      541 gcgaagataa ttaagaactc aggccaaaaa aaccggcgca atggccggtt tccgttgtta
      601 ctcaagcttt caaacgaagt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
      661 aggtctgata accggagttg atgacatgtc cagtatcatc taacgcgaca aagtaggttt
      721 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccacga gcatggatca
      781 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
      841 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagccgtac
      901 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
      961 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
     1021 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttatccacc
     1081 attcaacccg ctgtcgcttg tcgtttcagt agcgacgggt tagctttaag gaagttttgt
     1141 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     1201 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     1261 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     1321 cacgcttgcc ggtaagctgt gccagatggc gattaatgag ctgcgccagg agaccggcaa
     1381 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggccg acgaacaaga
     1441 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     1501 cgcggtattg gccagcgaac aggcgctgcg cgaagcgggc attgaactga gcgattttgt
     1561 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     1621 cagcggtgtc gtggtgggca ccgatcatgc agcagaagct attaccggat tcttcactaa
     1681 atatggtgac ggcggtacgg acattaatcc gctgtatcgt ctcaacaaac gtcagggtaa
     1741 acagttactg gcggcattag cttgcccgga acacctttat aagaaagcgc caacggccga
     1801 tctggaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     1861 tatcgacgac tatctggaag ggaaaaacgt acctcaacag gtcgccagaa caatagagaa
     1921 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt accgttttcg atgatttctg
     1981 gaaaaagtaa taatttgcac atattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     2041 atgcggcgtg aacgccttat tcgacctata aaactatgca aattcaatac attgcaggag
     2101 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat cgacccgcct
     2161 cttttttaat cgcttcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     2221 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     2281 ccgaacattt acgttcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     2341 atggtgaaag tgacaccatg ccgctctata tcggcaaaag cgttaacatc cgcagccgcg
     2401 tcctttctca tttacgtacc ccggatgaag ccgccatgct acggcaatcc cgacggatca
     2461 gctggatatg taccgccggt gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     2521 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     2581 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     2641 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag accatcgctg
     2701 atgaacaaaa actttgttat ggcctgctgg gactggaacc gttaagtcgc ggtcgtgcat
     2761 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     2821 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc
     2881 aaggggcggt ggcgctgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     2941 actggctgtg gctgggggcg gttaattcgc tggaagacgc gacaacgtta attcggacac
     3001 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     3061 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     3121 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc
     3181 ctggtcggta gtgagcaatt tatcgcccag ttgccacgcc ccgttaatca caaacaccac
     3241 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     3301 gacatccagg cgtgtcatga tgttgaaatc catcgacatc tgcccttccg tcagtttcgc
     3361 ttttaccacc tggtccgctg caaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     3421 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     3481 gccgggaaac aaagaaaact ctccattcgc cgcgatggat gcaatgctgg cacgccagta
     3541 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcatttcg
     3601 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     3661 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     3721 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     3781 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     3841 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     3901 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     3961 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     4021 gcgcttcagc ttttgcttta tcgaaggtat cgctgacaat gatgtcatgc attgcgcggc
     4081 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgcggatct
     4141 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     4201 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggca tgggccaggt
     4261 tagccgcgcc aagagccagg gtagaggcaa caaacagtgc agttaattta cgcatattct
     4321 atatccttcc tttcagttat ttattacggc tttcttaagt agcgtgccgt gttgacgaga
     4381 ttaactttac tgactttagc gtcaattaat cagagcaacg gtaaaacaat gaaagtgtaa
     4441 aaaacacttt tgcgccaatt atggagaaaa aaagaaaatt tgatggagag tgatgagaga
     4501 atattacaac acaatgattt tgcagagatt atgaagaact ataccggatg actggtgata
     4561 aataaagcaa ataaccagga ttaatctgta ttaatttata agaaagcaac ttaatacccg
     4621 cagaatgatt tctgcgggta agtattagct tattttttcg agcattaatc ccgcgcgtaa
     4681 tcccaacgct accaacggat tagggaataa cacatactct acatcatggg ttacggtaaa
     4741 acgttcctct ccgtcctgcg ccagcaatgt tcctttctca aacggcataa aattcagcgt
     4801 gtcacttgcc atatgcattt cgaaggacgg cgagtggcga gtaatttgcg aaaccacccg
     4861 ataacggagc ggcggtgttc tcacgatacc gacactctca ccagatagca gcgcagcaat
     4921 tgcgctggca gttactgcaa actggcgaag atcgttttgc ccaaagggca acgctttgcc
     4981 aagttccagc gtacaggcca gcgcgccaaa atgtctggcg ctgaaatggg taaacgtacc
     5041 accaggttcc tgatggaaca ccagcgcctc cagccccgcc gcacccagcc acgtcagaaa
     5101 tttctcgtcc cagggaatgt cgcgttgcgg taatacaccg aactgcggat gcaaggagcc
     5161 acgaattgcg gtatgtagat caaggtgcca gcgcacagat tctttgccct ggtcataaaa
     5221 atcttccagg cactgttcca gttcgcgcgc ccgacaggtt tctccgcttt cagcaaatag
     5281 ctgccaacga ccgccaaaca ttcgattcat atcgctatgg caataacgtt tcccttgctt
     5341 cagcgcagga ggattcccga ggatcaccag caaccgccaa cgtaacggga tctcgccgtg
     5401 agatatcgcg ccaagcaacg cgtccagcat ttccacaggt gccgtctcat taccgtggat
     5461 ccccgcagaa atcaccagcg cgccttgcgg tggcgttaat ggcgtcagtt ccagcacacc
     5521 atcgcccagc cagcgccagc gaacgccgtt gatttctcgc tcggtgataa ccgttttttt
     5581 acccgttaag gtcagagcaa gaaaattatc cattgccccc tccctcgcgc tggaacggat
     5641 aaaccgaacc gagattcagt aattggctca atacatccag tgcttcccgc ccttcgcgca
     5701 gcaattgcgg gtcggccaga tcggcagcag taagacgatc gcggtagtaa cgatccaccc
     5761 agtcattgag cgcattaaac aaggtatcgt tcatcatcac cgccggattc accgcccggc
     5821 gttcttcttc tgtcaatacc acccgcaacc gcaggcacgc cgggccgccg ccattcgcca
     5881 tgctttcacg taagtcaaag acttttagtt cgctaatcgg gttgtcagcg gcaaggagtt
     5941 cattgagata accccatact ccggcgtgtt cccgacactc ctgaggcagc accaacatca
     6001 tggaaccatc atcgcggctc aacagttggc tgttaaacag ataggtagac accgcatccg
     6061 acacggaaac ctgagttgcc ggaacttcta tcgccataaa accgttcacc cgcgcccgca
     6121 ggtttgccag taactgtgac tggcgagcga acgcctgttg gtggcaaaac agcacctggc
     6181 ggttacttac ggcaatcacg tcattatgaa aaacaccctg gtcgataacg tccgggtttt
     6241 gctgggcgaa aatcacctgt tggggattca cctgattaag ccttgtcacc gcctcgctgg
     6301 cttcgcgagt ctgtcgcgcc ggataacggg aaggccgggt atcattgcct tcttctcgcc
     6361 cgtagacaaa aagttgcata cccggctcac cgtaatgacc gccgagacga ttgtggtttg
     6421 ccgccccctc atcaccgagc aacgctacct gcggcaacgc cgaatggacg ctaaatttct
     6481 cttcgtcgtt aaaaatagct tttaacagcg attcagtgac gggcgcttcc agcgaacggt
     6541 gaaatttatt gttcaggttg gcaacggtga gatgcacttt gccatccagc gtatcggcag
     6601 atggcgcgat cgttgccgca ttggctaccc acattggcga agcggagctg acgctggaaa
     6661 gccagtgcgg tgcctggcgt gcaacttttt ccagtacctg ctcatcgcta ccactgaatc
     6721 ccaactgacg cagcaccgga ataaacggac gctcgtgcgg cgggatcacg gcctggggga
     6781 atcccgcatc ggcaagggtt ttcattttca gtaagccctg tttcgccgcc agtcgcgggt
     6841 tagacacctg aaaacggtga cgggtagagg cttcattacc aaacgacagg ccagcgtaat
     6901 gatgcgtcag ccctaccagc ccgtcgaaat taacttccca ggcgttcatc gcaccacctc
     6961 atcggaaaaa tccagcccgg ggttaagcgt tgcgggcaaa gttaacgagt ccgactccag
     7021 gctcgccata ggccatgtgc agtaatctgc ggcataccag gcgctggggc gatggttacc
     7081 ggatgcacca atgccgccga atggcgcggt actggcagca ccagtaagcg gtttgttcca
     7141 gttaacaatc cccgcccgcg cctccagcaa cagttgatcg aatttttccc gctcggggga
     7201 aaccagaccg caagagagtc cgaagcgagt gttattcgcc attagaatcg cttcctcgaa
     7261 agaatcataa cgccagacgc gcaataacgg tccgaacacc tcttcatctg gtacgccagc
     7321 aacgcctgtc atttcaatga tccccggcgt cagcaacgat gtctctgatt gtaataagcg
     7381 cggcgcaagc agggttcgtc cgcccatcgc ttccagttgc tgccaggcag taaccacctg
     7441 ctgtgcggcc tgttcagaaa tcagcccgcc aataaacggc tgcggttcgt catcccagtt
     7501 gcccggcgtt aatcgctggc tgacggcaac cagacgagca agaaacgcat cgccctgcgc
     7561 tccgcttttg agcaataaac ggcgggcgca ggtgcagcgt tgcccggctg tgacaaacgc
     7621 cgactgaatg gtcagatgga cagccgcgtc gatatccgcc acctcatcga taattagcgg
     7681 attattaccg cccatctcaa gggcgagaat tttctccggc tgaccggaga gctggcgatg
     7741 cagctggtag ccggtattgg cgcgaccggt aaacagcaaa ccgtcgagat cctccagcgc
     7801 actcagcgcc tgacccgttt cacgcccgcc ctgcaccagg ttcagcacgc ccggcggcaa
     7861 gccagcctgc tgccataaac gcattaccgc ttcgccactc cacggtgtca gttcgctggg
     7921 tttaaagata atggtgttac ctgccagcaa tgccggaacg atatgtccgt tcggcaaatg
     7981 accagggaaa ttatacggcc caaacaccgc cagcacgccg tgcgggcgat gtcgcaggct
     8041 cgccgcaccg tccggcattt cactacgctg ctcgccggta cgaacgtgat acgccttaat
     8101 tgatatcgcg attttattga tgttgccgct tcccagcgcg gcttacccgt ttctctggca
     8161 ataatcgcgg ttaattcggc tttattgctt tccagcaatc cggcaaagcg ttcgacaacg
     8221 acctgacgtt cagccaatga gagccgcgcc cagcgcggaa acgccgcacg ggctgcccga
     8281 caagcctgtt cgacctgagc ggcatcggca tcattgcctt gccataacac ctcgcccgat
     8341 accggattac gcttcacacg cgatgcgccc tggcccgtta tccagtcacc gttaatccat
     8401 aaagtcatgc tgttttctcc actgcgcaca ggcgcaccag acgaacgcga tcccctgcgt
     8461 ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg
     8521 tacgcgccag caccacgcgg aaatggtgat aattttcatt ggcgaccagg caggccggaa
     8581 aatcgccctg cgcaggctgc ccttctgcca cttccaccag ccgactttta cggatggcgc
     8641 gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc gaagatgtcg atatagttgc
     8701 ggtaacgaaa accttctttc tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct
     8761 gaccgatgac gtcctgggct tcctgggata aaaagtgggt atagatcgga tgtttcggca
     8821 tcagttctgc aataaatgcc ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat
     8881 ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa
     8941 tcaccccgcg catttcagca accactttgt cattaaactt gtcgcgaaaa gccgccataa
     9001 acataaagcg cgatttcgac agcaaatagc cgttgccctc tttgcgccag tccgggtcga
     9061 gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt actaagaaac agcgtcggca
     9121 atgcgttata gacattcagc tcttttgagg cgtgaaccaa cgtgccgacc cgatagttgt
     9181 gccagggatc gttcagccca accgccacct caatggcgca aatccccgcc acggtgcctg
     9241 tctcgctatc ttccagcacg aacacatagc cctgctcgct tttgggcagt tcgccttgcc
     9301 aggttttgat tgccctttcg atacgcgcgg aaagcgtggc ttcattagcg ggaagcgacg
     9361 tcaggccgcc gcccgttttg ctggcaagct gcatcagcgt cgagacatct gagcgctcaa
     9421 cgggacggat gaccatcatg atgaacctcg gctaacaaag tgttcgcaag cagctgcaaa
     9481 gcgatccagt ccggtcgtca cctcttcttc gctgacattg agcgcaggcg caaaacgcac
     9541 cacgttgcca cccgcaatca gtaccataac gcctgctttc gccgcctcct gagagatctg
     9601 tttcgcttgt ccggcgtaat cggcattcag tacacagcca atcaacaaac ctaagccgcg
     9661 aacttcactg aacaaaccgt agcggtgatt aatggtatta agacgctcaa caaaccagtc
     9721 gtgacgctgt ttaacgccat taagcatctc tggtgtgttg atgagctcca gcactttgcc
     9781 cgccaccgcc gaggccagcg gattgccgcc ataggtggtg ccatgagtgc caacggtcat
     9841 cacgctggcg cactcttcgg ttgttaacaa cgcaccgacc gggaaatcgc cgcccagcgc
     9901 tttggcggta gttaacagat caggtgtcac gccgtagtgc atataggcat acagttcccc
     9961 ggtgcgcccg acgccggttt gtacttcatc aaaaatcaac agcgcattgt ggcggtcaca
    10021 caattcacgc agaccttgta aaaacgcgtt gctggctggc accacaccgc cttccccctg
    10081 aatgggttcg acaatcaccg cacaggtggc gtcgtcaatc agcgcgctgg cagagttaat
    10141 atcgttatat gcagcatgac ggatatctgg tggcagtggc gcaaaatcct gtgaataggc
    10201 tggctgccca cccgcactga cagtaaacag cgtgcgacca tgaaacgcat ttttaaacgc
    10261 catgataccg ctcttatggc tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag
    10321 cgccgcttcg ttggcttccg caccggagtt acaaaagaag acgcgatcgg caaacgtggc
    10381 gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg
    10441 ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt tccggatgcg catggcctag
    10501 cgcgttcacc gcaatgccac ccgcgaagtc gatatactct ttcccctgct gatcccacaa
    10561 gcgcgaacct tcgccacgta ccggtataaa gggtgccgga gcgtaaacag gtatcatcca
    10621 ttcatcaaag ttttcacgcg taattggctg agacatagcg acctctacag taaataattc
    10681 gttatttata tgttgacaat aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt
    10741 gccagccagt gataaaagtg cataaacggc ggaggctaac tggaaatcaa ggagttataa
    10801 ccaaaccata tgcatttaaa gtgcatataa agtgaatacg tttgcgatgt gggtgaataa
    10861 aaagaataaa aaacgcaatg ttatgcagaa gtaaaatata attctggaat tgtgatcatt
    10921 gacgaaattt actggaaatt actgcgccat tctgacgcag cgcgcaccaa aagcgggcat
    10981 tttttgcgcc atcgttgaca tcattaacaa ccatcgatca aatcacttaa caacaggcgg
    11041 taagcaacgc gaaattctgc taccatccac gcactcttta tctgaataaa tggcagcgac
    11101 tatgaaattt gtctctttta atatcaacgg cctgcgcgcc agacctcacc agcttgaagc
    11161 catcgtcgaa aaacaccaac cggatgtgat tggcctgcag gagacaaaag ttcatgacga
    11221 tatgtttccg ctcgaagagg tggcgaagct cggctacaac gtgttttatc acgggcagaa
    11281 aggccattat ggcgtggcgc tgctgaccaa agagacgccg attgccgtgc gtcgcggctt
    11341 tcccggtgac gacgaagagg cgcagcggcg gattattatg gcggaaatcc cctcactgct
    11401 gggtaatgtc accgtgatca acggttactt cccgcagggt gaaagccgcg accatccgat
    11461 aaaattcccg gcaaaagcgc agttttatca gaatctgcaa aactacctgg aaaccgaact
    11521 caaacgtgat aatccggtac tgattatggg cgatatgaat atcagcccta cagatctgga
    11581 tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt accggtaaat gctctttcct
    11641 gccggaagag cgcgaatgga tggacaggct gatgagctgg gggttggtcg ataccttccg
    11701 ccatgctaat ccgcaaacag cagatcgttt ctcatggttt gattaccgct caaaaggttt
    11761 tgacgataac cgtggtctgc gcatcgacct gctgctcgcc agccaaccgc tggcagaatg
    11821 ttgcgtagaa accggcatcg actatgaaat tcgcagcatg gaaaaaccgt ccgatcacgc
    11881 ccccgtctgg gcgaccttcc gccgctaatt tagcagctct cctggctcaa actgggtcag
    11941 gagaattaac cttgagaaaa atcaacaaac tgtcagtaat gatttgttgc ctgccgtcct
    12001 ttgttatacc gtcactgcgt ttttagttgt ctgaccactt ctctattatc aagtttgata
    12061 taggaaactc cacgatgaat gctgagcgta aatttctttt cgcctgtctt atttttgcgc
    12121 tggtcattta cgctatccac gctttcggtt tattcgatct gctcaccgat ttaccccact
    12181 tacagacact cattcgccag agcggattgt tcggttatag cctctatatt ctgttattca
    12241 tcattgccac cctctttctg ttaccaggaa gcatactggt gatcgctgga ggaatagttt
    12301 ttggcccgct cttagggaca ctactctcat taattgccgc cacgctggcc tcctcgtgct
    12361 cattcctgct ggcgcggtgg ctgggacgtg atttactgct gaaatacgtg ggccatagcc
    12421 ataccttcca ggccattgaa aaaggcattg cgcgtaacgg tattgatttt cttattctga
    12481 cccgcttaat cccgttgttt ccttacaata ttcaaaatta cgcttacgga ttaaccacca
    12541 tcgccttctg gccttatacc cttatttcgg cactcacgac cctgcccggt attgttattt
    12601 ataccgtgat ggcaagcgat ctcgccaatg aaggcattac gctgc
//
LOCUS       DAJTMK010000120        12530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTMK010000120 DAJTMK010000000
VERSION     DAJTMK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12530
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.121"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1328
                     /gene="bcsG"
                     /locus_tag="OO135_002876"
     CDS             <1..1328
                     /gene="bcsG"
                     /locus_tag="OO135_002876"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5398655.1"
                     /translation="IGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFSLW
                     PAGQPTTTVTTTGGNAAATVATTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEAKR
                     KSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATSYS
                     GPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLKEV
                     RENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNTLP
                     LHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGDRM
                     QVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGKIF
                     TEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1415..1522)
                     /locus_tag="OO135_002877"
     CDS             complement(1415..1522)
                     /locus_tag="OO135_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5398656.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1998..3269
                     /locus_tag="OO135_002878"
     CDS             1998..3269
                     /locus_tag="OO135_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5398657.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKAFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLHGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(3299..4303)
                     /gene="dppF"
                     /locus_tag="OO135_002879"
     CDS             complement(3299..4303)
                     /gene="dppF"
                     /locus_tag="OO135_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5398658.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(4300..5283)
                     /gene="dppD"
                     /locus_tag="OO135_002880"
     CDS             complement(4300..5283)
                     /gene="dppD"
                     /locus_tag="OO135_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5398659.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(5294..6196)
                     /gene="dppC"
                     /locus_tag="OO135_002881"
     CDS             complement(5294..6196)
                     /gene="dppC"
                     /locus_tag="OO135_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5398660.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(6206..7225)
                     /gene="dppB"
                     /locus_tag="OO135_002882"
                     /pseudo
     CDS             complement(6206..7225)
                     /gene="dppB"
                     /locus_tag="OO135_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
     gene            complement(7460..9067)
                     /gene="dppA"
                     /locus_tag="OO135_002883"
                     /pseudo
     CDS             complement(7460..9067)
                     /gene="dppA"
                     /locus_tag="OO135_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            complement(9812..9888)
                     /locus_tag="OO135_002884"
     tRNA            complement(9812..9888)
                     /locus_tag="OO135_002884"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9852..9854),aa:Pro,seq:cgg)
     gene            complement(9980..11671)
                     /gene="eptB"
                     /locus_tag="OO135_002885"
     CDS             complement(9980..11671)
                     /gene="eptB"
                     /locus_tag="OO135_002885"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5398661.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHILQAKEAAAN"
     gene            complement(11924..12448)
                     /gene="lpfE"
                     /locus_tag="OO135_002886"
     CDS             complement(11924..12448)
                     /gene="lpfE"
                     /locus_tag="OO135_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR5398662.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENITPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
BASE COUNT         2997 a         3346 c         3151 g         3036 t
ORIGIN      
        1 tgattggggc catttttgtt ttattagtgg cctggttatt cctgtcacaa tggattcgca
       61 ttaccgtttt tgtggttgcc atactgctat ggctgaacgt acttaccctg gcgggaccaa
      121 gtttctcctt gtggccagcc ggacaaccga cgaccactgt aacaacgacg ggtggtaacg
      181 cagcggcaac cgttgcgacg acgggtggcg caccggtagt gggtgatatg cccgcacaaa
      241 ctacaccgcc aacaacggcg aaccttaacg cctggctgaa taatttctat aacgcggagg
      301 cgaaacgtaa atcgaccttc ccgtcttcgc tgcccgctga tgctcagcca tttgaactac
      361 tggtgattaa catctgttcg ctttcctggt cggatataga agccgccggg ttgatgtcgc
      421 atccactgtg gtcgcatttc gatattgagt tcaagaactt taactccgcc acctcctaca
      481 gtggcccggc ggcgatccgt ttactgcgcg ccagctgcgg gcagacttcg cacactaatc
      541 tgtatcaacc ggcaaataac gactgctatc tgtttgataa cctttcgaaa ctgggcttta
      601 cccagcacct gatgatggga cataacggcc agttcggcgg ttttttgaaa gaagttcgcg
      661 aaaatggcgg catgcagact gaattgatgg atcaaacaaa tctgccggtt attttgctgg
      721 gctttgatgg ttcgccggtt tatgacgata ccgccgtgct taaccgctgg ctggacgtta
      781 ccgaaaaaga taaaaacagc cgtagtgcca cgttctacaa cacgcttcca ctgcatgacg
      841 gcaaccatta tccgggggtc agcaaaacag cggattacaa agcgcgggcg cagaaattct
      901 ttgatgaact ggacgccttc tttactgaac tggagaaatc gggtcgtaaa gtgatggtgg
      961 tcgtggtgcc ggaacacggc ggcgcgctga agggcgacag aatgcaggta tctggcctac
     1021 gtgatatccc tagcccgtct atcacagacg tccccgttgg ggtgaaattc ttcggcatga
     1081 aggcaccaca tcagggggca ccgattgtca tcgaccaacc gagcagcttc ctggctatct
     1141 ccgatctggt ggttcgcgtt cttgatggca agattttcac cgaagacaat gttgactgga
     1201 aaaaactcac cagtgggttg ccacaaacag caccggtctc cgagaactca aatgcagtag
     1261 ttattcaata ccaggataaa ccgtacgttc gcctgaacgg cggcgactgg gtgccttacc
     1321 cgcagtaagc acgaaaaagg ccgcagagtt taacctctgc ggcctgatcc gggcgcaaat
     1381 tgccattacg gcagcctgac gcccgcatga cacgttactt ccgcttattc agccagttca
     1441 cgatcatact ggcaagaatg ccagcaatga ccggagccgc taaatcatgc cagaaggcca
     1501 tgcccagttc tgcgagcgtc atatagccgc ctgtgttgta atgccaacgt ttcgcggcta
     1561 ttcttgagtt gtttgggttc aagattagcc cccgtgatgt tgtcaggtgc atacctgcaa
     1621 cgtgcggggg ttttctctct ccagcaacca atgccaccag ggataaagcc cccgcaacat
     1681 tgcgcctcac cggaaccctt tcggcttgcc gctgatttta cgattgcttt ttactgtcag
     1741 cacccggcaa tcttcctcat tgcgcgatgc cttccagctt aatttcttta tttgcaatag
     1801 ttgcagttca tatccgggct gactccagac tggtagtgaa atgcgcaatt ttgtagaatt
     1861 gcgcctcctc gcgacctccc cagtgaatct gttgcctcat tttttacata ctgcgtattc
     1921 gacttctcca cctgttgcgc aagagaaact gggtttattc atttttgcga ggccgacttc
     1981 tttctggaca ggactttatg cagcacaaca cactatcgaa acacaatcag aaattgccgt
     2041 ttacacgcta cgacttcggc tgggttttat tatgcatagg catggcgatt ggtgccggaa
     2101 ccgtgctgat gccagtacaa attggcttga agggaatttg ggtatttatt accgcagcga
     2161 tcattgctta tcctgccacc tgggtagtgc aggacattta tttaaaaacc ctttctgaaa
     2221 gcgattcctg taatgactac accgatatta tcagtcatta cctggggaag aactggggaa
     2281 ttttcctcgg ggttatctac tttttgatga ttatccacgg gatttttatc tactctctct
     2341 ccgtggtttt cgacagcgcc tcgtacctga aagccttcgg tttaaccgat gccgatcttt
     2401 cacaatctct actttataaa gtcgctattt tcgccgtact ggtagcgatt gcgtctggtg
     2461 gtgaacgatt actgtttaag atttccgggc caatggtggt ggtcaaagta gggattattg
     2521 tcgtgttcgg ttttgcgatg atcccgcact ggaatttcgc caatataacc gccttcccgc
     2581 aagcctccgt ctttttccgc gatgtcctgc ttacaattcc attttgcttc ttttctgcgg
     2641 tatttattca ggtacttaac ccaatgaata ttgcctatcg taaacgggaa gcagataaag
     2701 tactggcaac ccggctcgcg ctgcgtaccc accgaattag ttatatcacg ctcatcgcgg
     2761 tgatcctgtt ttttgccttt tcgtttacct tctcaattag ccacgaagaa gccgtttctg
     2821 cctttgaaca aaatatctcg gcactggcac tggccgcgca ggtgatccct ggacatatca
     2881 ttcatatcac ctctacggtg ctgaatatct ttgccgtact gaccgcattc tttggcattt
     2941 atctcggttt ccacgaggct attaaaggca ttattctcaa tctgttaagc cgaattattg
     3001 ataccaagaa aattaactca cgcatgctga ctctggcgat ctgcgctttt atcgtcatta
     3061 cgttgacgat ttgggtttcg tttcgtgtat cggtgctggt gttctttcag ttgggaagcc
     3121 cgttatatgg cattgtgtcg tgcctcattc cgtttttcct gatctataaa gtcgcacaac
     3181 tggaaaaact tcacggattt aaagcctggc taattctgct ttacggcatt ttgctatgct
     3241 tgtcgccact gttgaagctg attgagtaaa ccggagcgca tggccccggt tttgtgagtt
     3301 aacgctgcgg attttcatcc tgatcaacag caaaacaagc taccagttga ccgccgtagt
     3361 cttttagctg cggctgcaac tgggtgcagg ggccgaagcg ccgacgacag cgggcgttga
     3421 aggcgcaacc cggcggtgga ttcagtgggc ttggtagttc accgctgagc ttgatgcgct
     3481 cgcggcgatc gtccgggttc aggcgcggcg tcgcggaaag tagcgcctga gtgtacggat
     3541 ggcgcgggtt attgaagatt tggtctttcg ttcccttctc cacgcagcgg cccaggtaca
     3601 tcaccatcac ttcatcagca atgtgctcca ccaccgacag gtcgtgggag ataaagacat
     3661 aagacagccc caactcctgc tgcaaatcca tcatcagatt cagcacctgc gcgcgcactg
     3721 aaacatccag cgcggaaacc ggttcatcgg caatcaccac atccgggtcg agcatcagac
     3781 cacgggcgat ggcgatacgc tgacgctgac cgccggagaa catatgcgga tagcggtcat
     3841 agtgctcggt tttcaggccg actttcgcca tcatcgacag ggctttttcc cgacgctgtt
     3901 ctttgcttaa gctggtgttg atcagcagcg gctcttcaag aatttgcccg acttttttac
     3961 gcggattcag cgaaccgtaa gggttctgga agacgatctg gattttctgc cgacgcagct
     4021 tctgcgcctg cggatcgtgc ttaagcagat cctgcccctg gtaatacagc tcgccaccgg
     4081 tgggcgtttc aatcatcgtc agcaaccgac cgagggtcga tttaccgcag ccagattcgc
     4141 ccactactgc cagcgttttg ccacgttcaa ggttaaacga aacgccatcc agcgctttaa
     4201 ccagacgttc cggcgcgaac atgcctttct tcaccggata atgttttttc aggtcgatag
     4261 cctgcaacag cggttgttgc agggtggcct cttgcgtact catagtgtcg gcctcccggc
     4321 atcatcaagt gggtaatggc atttggactg acgcccgtca gcgagcatat tcagcgccgg
     4381 ttcttcagcg cgacatctgt cagtggcata ggggcagcgc gggttaagca ggcagccgtt
     4441 cgggcggtcg tacttgccgg gaacgacacc tggcaacgac gccagacgtt ctttgtcctg
     4501 agcaaattct ggcagcgcac gcagcaatgc ctgagtatac gggtgacgcg gcgcatggaa
     4561 gatggcgtgc gcatcgcccg tttccaccac ctggcctgca tacatcacaa tgattttatg
     4621 cgcagcttcc gccaccagcg ccaggtcatg ggtaatcaac accagcgcca tgttctcttt
     4681 ctgctgtagc tccagcagta gttcgatgat ttgcgcctga atggtcacgt ccagcgcggt
     4741 ggtcggctca tcggcaatca gcagttttgg tcgacaggca atcgccatgg cgatcatcac
     4801 gcgctggctc atgccgccgg aaagctgatg cgggtaaaca tccagacgcg aagccggatc
     4861 gggaataccg acctgattca gcaggtcaat cgcccgctga cggcgggtac ttttgttgcc
     4921 gccctgatgc accttaatcg cttccatgat ctggaaaccc acggtgtagc acgggttaag
     4981 gctggtcatc gggtcctgga agatcatcgc cacttcggca cccaccaggt tgcggcgctc
     5041 tttttctgat atacgctgca aatcctgacc gttaaacacc agtttttccg ccattacgcg
     5101 gcccggataa tcaatcagcc ccataatcgc cagggaactg accgacttac cggagccgga
     5161 ctcacccaca atcccaacca cttcgccctg ttttacgctg tagctgatgc ggtctacggc
     5221 gcggaacggt gcgctttcgt cgccgaaatg caccgataat ttatctacat ttaataacgc
     5281 catctcgaac ctcttactgc ttcagtttgg gatcgagcgc gtcacgcaga ccgtcaccca
     5341 tcaggttaaa tgccagcacc gtcagcagga tcgccagacc cgggaaggtc acgacccacc
     5401 aggcgctttg cgcgaactgc aacacgtcgg agagcatggt gccccactcc ggcgttggcg
     5461 gctgtgcccc catgccgagg aaaccaagag cagccatatc gagaatggcg ttagagaaac
     5521 cgagcgacgc ctgaacaatc agcggcgcaa ggcagttcgg gaagatgtta ataaacatct
     5581 gacgcatcgc cccggcaccc gccacgcgag acgcggtgac gtaatcgcgg ttaacttcca
     5641 ccagcacggc ggcgcgggtt aagcgcacat agtgcggcaa ggcaacgaag gtcagtgcca
     5701 gcgcggcgtt accaatcgac gggccgaaaa ttgccaccag caccagcgcc agcagcagac
     5761 ttggcagcgc cagcatgata tcgaccacgc gcataatgat gttatcgacc aggccgccaa
     5821 agtaaccggc gatcaggccg agaataacgc ccataatcag cgataacaca actaccagac
     5881 agccaaccag cagcgacagg cgcgcaccgt acatcaggcg cgacagcata tcacggccta
     5941 cgtcatcggt gcccagcaag tgcgccatgc tgccgccttc ctgccaggct ggcggggcga
     6001 gcagtgcatc gcggaactgt tccgccgggt tatagggtgc aatccagttg gcaaagatcg
     6061 cgatgaacag cacgatgacg acgtaaacca gcccgacgac cgcgcctttg ttgcgtttaa
     6121 aatagtgcca gaactcctgt aacggggtca tcggcaccgg tgcgctaatc actttatttt
     6181 cagtaacctg tgacatgatg tccccttact tcttatgacg aatacgcggg ttcaccacgc
     6241 cgtacagcag atcgaccagc aagttgacga ggataatcat cgtcgccacc agcaatacgc
     6301 cgccctgcac taccggatag tcgcggcgtt gcagtgcgtc aatcaaccag cgtcccagac
     6361 cgggccacga gaagatggtt tcggtcagaa tcgcccccgc cagcaatgtt cccacctgca
     6421 ggccgataac ggtcaccacc ggcagcatcg cgttacgcag cgcatggacg ataatcaccc
     6481 gcatgcgggt tagccctttg gcgcgcgcgg tgcggatgta atcctcgccc agcacttcca
     6541 gcatcgagga gcgtgtcata cgcacaatga ccgccagcgg aatagtaccc agcacaatgg
     6601 caggcaagat catatgggcg acggcatcga taaagttgcc gtcttcaccc cagatggcgg
     6661 tgtcgattag cataaaaccg gttaacggat tggagtcatc gaggaacacc atatcgctca
     6721 cgcgaccgga gacgggcgtc aggttccagt gcaccgaaac cagcatgatc agcatcatgc
     6781 cccaccagaa gatcggcatc gagtagccgg tcagcgccag gccaactgca gtgtgatcga
     6841 aaatggaacc gcgtttaacc gcagccagca caccaaccgg aataccgact gccgtagcaa
     6901 aaatcatcgc gcagacgcca agttccagcg tggcctgaaa gcgcggcacg aactcttccc
     6961 atactggaat tcggcttttc attgaaatgc ctagatcgcc gtgcattacg ccctaaatgt
     7021 aatggagata ctgctgccac atcggtttat ctaagccgag ttcagccagc agctgcgcgt
     7081 gacgctcagg ggagatccca cgttcgcctg ccatgatcat caccggatcg cccgggatca
     7141 tgtggacaaa ggcaaatgtg agaagggtaa taccgataaa cgtggggatg acgagtccca
     7201 aacgtcggag aataaactgc aacataaccc ggattctcta tagtgacccg cagcctggaa
     7261 ctgcgtgtct gtattgctca caaatcaatt cccgcccttg tcggacagag aatgaagtat
     7321 tgctgccgga tgcggcgtta acgccttatc cggcctacaa aatcgtgcaa attcaataca
     7381 ttgcagaatt ttcgtaggcc tgataagcgg agcacatcag gcagttttgc ctttaccatc
     7441 agtcttgtat ggcttttaat tattcgatag agacattttc gaagtgatgt ttgcctaatg
     7501 gatcaaccac atagcctttg acttctttgc gtaccggttc aaacacggtg gagtgagcga
     7561 tgatcagtgc cggagcctga tcgtgcatca ccacctgcgc ttgcttgtac agttcaacgc
     7621 gtttgttatg gtcgtcggta gcacgcgccg gttgaatcag atcttcaaac ggtttgtagc
     7681 accattttga gtagttagaa ccttgttcag aggcggcgca gctgaacagg gtggcgaaga
     7741 agttatccgg gtccccgtta tcgccagtcc agcccatcat taccgtctga tgttcgccat
     7801 ctttcgcacg cttgaggtac tcaccccatt cgtaggtgac gattttggcc tgcacgccca
     7861 ctttcgccca gtctgcctga atcatctccg ccatgcggcg agcgttcggg ttatacggac
     7921 gttgtaccgg catcgcccac aggtcgatgg agaaaccttt ttccagaccc gcttctttca
     7981 gcaaggcttt cgctttttca ggatcgtagg tgtaatcctg aacgtcgtcg ttatagcccc
     8041 acatggttgg cgggatcagg tttttcgctg atacacccgc gccctgataa accgctttga
     8101 tgatcgcgtc tttgttcacc gcgtaggtca gtgcctggcg aactttcacg tcatccagcg
     8161 gttttttctg cacgttatac gagaggtagc cgacgttcag ccccgacatt tccatcaggt
     8221 tgatggattt atcctgcttc atgcgagcga tatctgccgg gttcgggtac ggcatcacct
     8281 ggcattcatt cttctgcaat ttcgcgtaac gcacggaagc atcaggggta atagagaaga
     8341 ccagcgtatc gatctgcggt ttggtgcccc agtagccatc aaacgctttg tagcggatac
     8401 gggaatcttt ttggtactgc tgtaactgga acggaccggt tccgattggg ttgaggtcca
     8461 gtttttccgg tgtaccggct ttcatcatcg catcagcata ttcttttgac agaatagagg
     8521 cgaagtccat tgccagatca gcgaggaacg gcgcttccgg gcgagtcagt acgaactgaa
     8581 ctgtgttgtc gtccaccttt ttcacttcac tgatcagctc cggcaagccc atgccttcga
     8641 agtattcgta gctgccgcca gagactttat ggtacgggtt ttgcgcgttt ttctgacggt
     8701 cgaacgagaa caccacgtca tcggcgttca gttcacgcgt cggtttgaat tctttattgt
     8761 cgtgccactt cacaccttta cgcagatgga aggtatagat tttaccgtct tagctgactt
     8821 cccacttttc agcgaggccc ggtatcactt cagtggtgcc gattttaaat tcaaccaggc
     8881 ggttataaag cggtacggaa gaggcgtcat aggtggtgcc ggaggtaaac agctgcgggt
     8941 taaacccttc cggagatcct tctgagcaat aaaccagagt tttagcctga acacttgctg
     9001 cgacggtcat agccaccagg ctgagaccaa gcttcagcat ccctgacttt ttcaaggaaa
     9061 tacgcattat tctgctccaa ttgtgatgtt tgttgtttta accctttgca gtgggttgtc
     9121 gctgcctgac cttttttgtt ttttgcccgg tcgggtcaac gttatgaggt ggggatgccg
     9181 tactaattaa catcagtgta acagtgcgga tcctccataa aatgcccctc gtgacctaca
     9241 atctgtcaac agaatgtgaa aacgtcaata cagccaaccg ggatttacac caacggtgag
     9301 aatccacaaa caaagattaa aaaaacttca aacagctatt tgcagcagag aaatttgtgc
     9361 tactccaaca taaccagaac aatcagctta atatttagca acatttggtg attaagtttt
     9421 atgcatttaa tgaaaaaaat ctgaggaaaa ggtggatatc tgagcgatta ataccatgaa
     9481 cgctaaaacg cacagcggaa aatgccagcg ccagccataa gtaacgcaaa aaaagattta
     9541 agcaaatata aaaaaagaca atggtttctg tcacaaatcc gttaatggat agtgagatat
     9601 ggggcgcaaa gttggcagga aaatgtgtgg tgtgttggtc gaataaggcg gtcgcgccgc
     9661 atccgacaat aagtgcggtg ccgttgcctg atgcgacgct ggcgcgtctt atcaggccta
     9721 cgtcgagtcg aacctcccag cccccggagg ttctcatcct catgggcttc aaatgcaaaa
     9781 aagcctgctc gctgagcagg ctttttgaat ttggtcggtg atagaggatt cgaacctccg
     9841 accccttcgt cccgaacgaa gtgcgctacc aggctgcgcc aatcaccgaa tgcggggcgc
     9901 atcttactgc gcagatacgc cctcgtcaat cccttaatag caaaatgcct tttgatcggc
     9961 gagaaagtca gcaggccgct tagttagccg ctgcctcttt tgcctgcagg atgtgacacc
    10021 agttgttgtt ttcgttaatt ccaccatccg gtgaagtata gccaagacaa cccatgatgg
    10081 tatcgtacag ctctacgtga cggcgtggca ctttcatgtc ggcttctttt ttcagctgcg
    10141 caaatgcctg cgcattggcc ggattttcca gatatttatc tgacatccag accatcatcg
    10201 gtacgcggaa ctgctccggc ggtgccaatt cacgcggcgt gccgtgcagg tgctcgcgtt
    10261 cattaattga ctcaccgtgg tcagctgcgt agaacacaat cgctttctta tcgcgaacct
    10321 gatcaatcac gctggagata aagtgatcca cataggtcac cgagttgtca taggagttga
    10381 tcatctgcgc tttggtacag ccgctatcaa caccaataca ttccggcttc cactgcgcga
    10441 agctacgcgg ataacgctgg gtgtagttaa aatgcgaacc tttggtatgc agaatgatca
    10501 gatgcttacc atccgggttg cgccctagcg attgctgcat ttcgtctacc agcaacatat
    10561 catctaccgg cttgccacga ttacgtggct ccgcaccaat ctgctcacga taagcaatgt
    10621 tgtccgccat cgtgttgctg tagaaccaca tctcgctctg catagcgtag aggtcagaac
    10681 tgaatcctaa ctgcttcaga accgcgaaaa tgttctgttc ttttaatgtg cgctgcggat
    10741 tatcttccgc gcccccctga cgtacaaaca tgcaacgcag tgagagtttg gttgcggtat
    10801 cacaggagta accacggaac gccgccagat ttttctcctg ggccagtttc ggcgtggtat
    10861 tacgctcata gccgaaaata cccatatggt cccaacgcgt ggtttcaccg atgataaaca
    10921 cgacataagt atcatcaacg ttttgtggag cctggtatgt aaacttcttc gccggattaa
    10981 gcaatgaatt attatcggaa gattcatcga cccgcgccca ggcatacagc cccaacgcag
    11041 aaagccagtt tgatggcaga taagagttcg ctactacacc gccataactc ggcaaatcaa
    11101 cgccggtcgc cctctccact ttcttctgct ggatatccag caaacgaatc ggtgcccaaa
    11161 ccataatacc cgccagtacg acgaccgcca ggctgcgaat acgctgccct ggggttcgca
    11221 gttgtcggag caaggtgtag cgacagcggt tattccagat aaggataaga ggcaatgcac
    11281 taacggcgat taaccagaga ataaagttca gaccaacaac ttcttttgac aggtcgatat
    11341 cggtggtcat gacggaagcg atgatgccat aaccaatgac cacattaagg aaggtcatgt
    11401 aatagctggc acctgcggaa aagagcacca ccagcgacgc cagaatacgc cagctgcggc
    11461 ggccaaacag cgatagaaga cgtagtaaaa agaaggtcac cagtacggtg gcggccagtt
    11521 caacaacagc agaaacgcct ttccagacgg taaaatcgtg cgcatagctg ccgaagcggc
    11581 gataaaaaac cgcgccattc ataaaaagac caatatagat tgcaagcaaa aagctcagct
    11641 tctgctgtgt aatcgatttg atgtatctca tgcaaacaaa ccctggacaa acaggtgata
    11701 acaccctgct tagcggcttt tcaggcccgg caggggaaaa gtgcggaggt agtcagataa
    11761 gtaaaatcta cacgcgcgaa taagtagacc acagcaaagc gaaaaagtgt ggggaaagag
    11821 tgtgcatgaa gcacaaattt acaaaaatgc aagtttgaaa agagacttta gcgttacaaa
    11881 aagcagtaaa aagggccagt taactggccc ttaagatgtg aaattattcg tactgtacag
    11941 taaagtcgac cgttgcatct gcgcgaccaa tagttgcatc ggcgcgaata ggcacgtagc
    12001 tggcgatata gttcagttga tgtgcgccat caccgttaat tgcaaactca accggtgtcg
    12061 gcgttatatt ttccgggaac tgttgtactc cagcagcggt tttgattttc agcccaacgc
    12121 ctgtagcgcc acccgcatcc agcgcaaaaa gatttgagtc gtctgcattg ggtgtaccgt
    12181 ccatagtcac gtaggctctg accacttcgc tacagttttt caggccgatg gaaaaaggag
    12241 tgggattaat ttccgttccg attcttaccg gctttttgag aaccgcggtg cccatgtcaa
    12301 cctcaacgct gtctttcacc agttcgcaag caccctggct gattttaagg gtgaatttca
    12361 atgttccttt cggccctaaa tccgtggttg ccgcgaatga cgaaaaggaa agaggcagca
    12421 ggcataacgc aagtattgtt cgcttcatga ctgttactcc agttcgacat gtatggtagc
    12481 aataccgcta taatccccgg cagcgggtaa ttttccggta gtgctaacgg
//
LOCUS       DAJTMK010000121        12409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTMK010000121 DAJTMK010000000
VERSION     DAJTMK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.122"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..336)
                     /locus_tag="OO135_002887"
     CDS             complement(<1..336)
                     /locus_tag="OO135_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HCR5398663.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(555..1036)
                     /locus_tag="OO135_002888"
                     /pseudo
     CDS             complement(555..1036)
                     /locus_tag="OO135_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1157..1429
                     /gene="rcnR"
                     /locus_tag="OO135_002889"
     CDS             1157..1429
                     /gene="rcnR"
                     /locus_tag="OO135_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5398664.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      1521..1617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1652..2440
                     /gene="thiM"
                     /locus_tag="OO135_002890"
     CDS             1652..2440
                     /gene="thiM"
                     /locus_tag="OO135_002890"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5398665.1"
                     /translation="MQVDLLSSAQSAHTLHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            2437..3237
                     /gene="thiD"
                     /locus_tag="OO135_002891"
     CDS             2437..3237
                     /gene="thiD"
                     /locus_tag="OO135_002891"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5398666.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            3302..4120
                     /locus_tag="OO135_002892"
     CDS             3302..4120
                     /locus_tag="OO135_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5398667.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            4172..4918
                     /locus_tag="OO135_002893"
     CDS             4172..4918
                     /locus_tag="OO135_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5398668.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(4892..5760)
                     /locus_tag="OO135_002894"
                     /pseudo
     CDS             complement(4892..5760)
                     /locus_tag="OO135_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(5757..6761)
                     /locus_tag="OO135_002895"
     CDS             complement(5757..6761)
                     /locus_tag="OO135_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5398669.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPPLKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(6758..8035)
                     /locus_tag="OO135_002896"
     CDS             complement(6758..8035)
                     /locus_tag="OO135_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HCR5398670.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            8292..9344
                     /gene="fbaB"
                     /locus_tag="OO135_002897"
     CDS             8292..9344
                     /gene="fbaB"
                     /locus_tag="OO135_002897"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5398671.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAYRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9652..10506
                     /gene="gatY"
                     /locus_tag="OO135_002898"
     CDS             9652..10506
                     /gene="gatY"
                     /locus_tag="OO135_002898"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5398672.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQINEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            10535..11797
                     /gene="gatZ"
                     /locus_tag="OO135_002899"
                     /pseudo
     CDS             10535..11797
                     /gene="gatZ"
                     /locus_tag="OO135_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            11807..12259
                     /gene="gatA"
                     /locus_tag="OO135_002900"
     CDS             11807..12259
                     /gene="gatA"
                     /locus_tag="OO135_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5398673.1"
                     /translation="MTNLFVRSGIYFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            12290..>12409
                     /locus_tag="OO135_002901"
     CDS             12290..>12409
                     /locus_tag="OO135_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5398674.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCR"
BASE COUNT         3180 a         3101 c         3202 g         2926 t
ORIGIN      
        1 gcgatgataa aaaatctcac cgtcgtaggc tttaatgatt ttaccgtcgg tgtcgctgat
       61 caacacgtac gcgccaccca tataggtcca gtgcgtcccg gcatcaggcg cgggcaggtt
      121 acgtaactgc cactgcttaa tgttgtactc atcggtgcgg tacatctctg ggacggtatc
      181 gcctggtttg aaatgcttaa agtcagcaat gaaactgctt aactcatact tagaaattcc
      241 cgaggtattg gtcgaacccg cggtggctgg tgcggcccag gcggcactgg taaccagcaa
      301 aagcgcaccc agcaacatct tatttttaat ggtcataaac tctccacttt ggggatctgt
      361 aatatcagtc gcatatcttt cctgccatac aatataactg acaagcgata ttttgttagt
      421 gatcccatga tttcgtaaaa agtgtaaatg aaaggatgcc ctaaaagcat ccttagcgag
      481 cagagagtca gcggaaatag ctgatttaag aatcatcctg ggctgaagat aacatgcaag
      541 cctaaaggat tgagttatcg cattatgccc atgaagccat gtacgcccat atacacaccg
      601 actaagccaa tcaacagact ggaaaaatag ggggcgcgtt tagcgagagt gttaaatccg
      661 ctccagcgtt ttgcgacctg ctgaacgctg attgctgcgc caacgcctac ggtgacaagc
      721 gttaacgcca ggccaatgct gaaactgacg accagtgttg cgcccagtgt cagggctttc
      781 aactgaatgc atatcagcgc ttactgatca ccatcccgcc aaaggcaatc aaccagacca
      841 ccgcggtatg cgaaatggtt gctgccagtc cgagcatcac cgcctgttta atggtgcctt
      901 tgatggcgat gataaacgcc gccatcatcg tttttgagtg ccctggttcc aggccatgaa
      961 gcgcaccaag taagatggcg ctggggatga agaaccaggc gtttccttgc tgaagaagag
     1021 ttgtaaattc ggtcatgata ataattctta gtattaattc ggcaatctga ttctactccc
     1081 ccccagtacc tgatactacc ccccagtaga ttaatagtgc tatgattttt catattcttg
     1141 ttaaccaggt gttgccatgt ctcatacaat ccgtgataaa cagaaactga aagcgcgtgc
     1201 cagtaagatt cagggccagg tcgtggcgct caagaaaatg ctcgacgagc cgcacgaatg
     1261 cgctgcagtt ttacaacaga ttgctgctat ccgtggcgcg gtaaacggtc tgatgcggga
     1321 agtgattaaa ggtcatctga cggaacacat cgttcaccag ggggatgagc taaaacgtga
     1381 agaagatctg gatgtcgttc tgaaggtgct ggattcatat atcaaataat ttattaacgc
     1441 gattgtaaaa ctgccgtttt tcctcgttta caacgcgtgc gctggacatt accatcctcc
     1501 tctgcgattt atcatcgcaa ccaaacgact cggggtgccc ttctgcgtga aggctgagaa
     1561 atacccgtat tacctgatct ggataatgcc agcgtaggga agtcacggac caccaggtca
     1621 ttgcttcttc acgttatggc aggagcaaac tatgcaagtc gacctgctga gttccgcgca
     1681 atctgcgcac acgttacacc tttttcacca acattcccct cttgtgcact gcatgaccaa
     1741 cgatgtggtg caaaccttta ctgccaatac cttgctggcg ctcggtgcat cgccagcgat
     1801 ggttatcgaa accgaagagg ccagtcagtt tgcggctatt gctagtgcgt tgctgattaa
     1861 cgttggcacg ctgacgcagc cacgcgcaca ggcgatgcgt gccgccgttg agcaagcaaa
     1921 aagctctcaa accccctgga cgcttgatcc agtagcggtg ggtgcactcg attatcgccg
     1981 ccgtttttgt ctggaacttc tgtctcataa gccaaccgcc atacgtggta atgcttcgga
     2041 aatcatggca ttagctggcg ttgctaatgg tggacgggga gtggatacca ctgacgccgc
     2101 agctaacgca atacccgctg cacaaacact ggcacgggaa actggcgcaa tcgtcgtggt
     2161 cacaggcgag gtggattatg ttaccgatgg gcatcgtatc gttggcattc acggtggcga
     2221 tccgctaatg accaaagtgg taggaactgg ctgtgcatta tcggcggttg ttgctgcctg
     2281 ttgtgcgtta ccaggcgata tgctggaaaa tgtcgcatct gcctgtcact ggatgaaaca
     2341 agccggagag cgcgcagtcg ccagaagcga ggggccaggc agttttgttc cacatttcct
     2401 tgatgcgctc tggcaattga cgcaggaggt gcaggcatga aacgaattaa cgctctgacg
     2461 attgccggta ctgatcccag tggtggtgcg gggattcagg ccgatcttaa aaccttctcg
     2521 gcacttggcg cttatggttg ctcagttatt actgcactgg tggcgcaaaa tacccgtggc
     2581 gtacagtcgg tgtatcgcat tgagcctgat tttgtcgccg cccagctcga ttcggtgttc
     2641 agcgatgtgc gaatcgacac cactaaaatc ggtatgttgg cggaaaccga tattgttgaa
     2701 gcggtggcag aacggttgca acgttatcag atccaaaacg tggtactcga caccgttatg
     2761 ctggcaaaaa gcggcgaccc gctgctttca ccttcggcgg ttgctacgct gcgcagtcga
     2821 ttattgccac aggtttcatt aataacgcca aacttgcccg aagctgccgc cttgctcgac
     2881 gcgccacacg cgcgcaccga acaggaaatg ctggaacaag ggcgatcgct gttggcgatg
     2941 ggctgtggcg cagtgctaat gaaaggtggt catctggatg atgagcaaag cccggactgg
     3001 ctgtttaccc gcgagggtga acaacggttt accgcaccgc gcattatgac caaaaacacc
     3061 cacggtactg gttgtacact ctctgcggcg ttggctgcac tacgcccgcg ccatacaaac
     3121 tgggctgaca ccgtacagga ggcaaaaagc tggctttcat cggcgttagc ccaggccgac
     3181 acgctggaag ttggtcacgg tattggtccg gttcaccact tccacgcctg gtggtgagca
     3241 ccgtactata gctgctttgg ttattgtcag cctttggcac tgacattcag ggatgaaacc
     3301 aatgcagcta agaattacca gtcgaaaaaa actcaccgct ctattatgcg ctcttgggct
     3361 aatttccatc gttgcaattt atccgcgtca gacggtgaat tttttctatt cgacagcagt
     3421 tcagattaca gactacatcc acttctacgg ttatcgcccg gttaaatctt tcgctattcg
     3481 tattcctgcc agttacacca ttcacggaat agatgtttcg cgctggcagg agcggatcga
     3541 ctggcagcgt gtggcaaaaa tgcgcgataa cggtattcgc ttacaatttg cctttattaa
     3601 ggcgacggaa ggcgaaaagc tggtggaccc ctatttttcg cgtaactggc aactaagccg
     3661 tgaaaatggc ctgctgcgcg gggcatatca ttatttctcc ccgtcggtag cagcaccggt
     3721 tcaggtgcga ttatttctgc aaacggtaga tttctcacaa ggcgatttcc ctgcggtact
     3781 ggacgtagaa gagcgaggaa aattatcggc aaaagaatta cgcaagaggg taagtcagtg
     3841 gctaaaaatg gtcgaaaaaa gtacggggaa aaagccgatt atttactcag gagccgtttt
     3901 ttatcacacg aatctggcgg gctatttcaa tgaatatccg tggtgggtgg ctcactacta
     3961 tcaacgtcgt ccggacaatg acggcatggc ctggcgcttc tggcagcatt ccgaccgtgg
     4021 acaggtagat ggcatcaatg gtccggtgga ttttaatgtg tttaatggca cggtggagga
     4081 gttgcaggga ttcgttgatg ggattaaaga aacgccttaa ataagaagca gtgatatact
     4141 cccaccagac tccctggcag gaaaaagaca aatggaacaa gcgcataccc agctgattgc
     4201 ccaactgaac gaacgtattt tagcggcgga taacacgccg ctttatatta agtttgccga
     4261 aacggtaaaa aatgccgtgc gcagcggggt gctggagcat ggcaatattt tgcccggtga
     4321 gcgtgattta agtcagttaa ccggcgtttc gcgcattacg gtgcgcaagg cgatgcaggc
     4381 gctggaagaa gagggtgtgg tgacgcgttc gcgcggttac ggcacgcaga tcaacaacat
     4441 cttcgaatac tcgttgaaag aagcgcgagg tttttctcag caggtggtat tgcgcgggaa
     4501 aaagcccgat acgctatggg ttaacaagcg tgtcgtgaaa tgtcccgaag aagtcgcgca
     4561 gcagctggcg gtcgaagcag gaagtgatgt ctttttgctt aagcgtattc gctatgtcga
     4621 tgaagaagcg gtatcgattg aggaatcgtg ggttccggcg catttaattc atgatgttga
     4681 tgccatcggg atttcacttt atgactattt ccgcagccaa cacatttacc cacaacgtac
     4741 gcgttcccgc gttagcgccc ggatgccgga tgccgagttt cagtcacata ttcagttaga
     4801 tagcaaaata ccggtgctgg tgatcaagca agtggcgctt gaccaacagc aacggcctat
     4861 tgagtacagc attagccact gtcgcagcga tctatacgtt tttgtgtgcg aggagtagtt
     4921 cctcgcgcgt tggcgcacaa tcaccgcccc gatgcccgac aacccacgac gccactgcat
     4981 tgcccagtaa tacggcatcc gccagtggca gaccagaggc cagcccggca agtacgccac
     5041 cggcatgact gtcgcccgcc ccaatggtgt cgacaacttg cgttggaaat gccggaatgc
     5101 agccagaagc gtcgttgctg aaataccatg cgccttcttt atcgagacga acgatcaacg
     5161 gcgcggcaaa tttctcctgc cattgcttgc caagtgttgt tatctctgcg gataaagcaa
     5221 aacgttcggc ggcaatctca gcctcttgac gattgagtga cactaaaggt cgacaggcca
     5281 tggtccgcgc cagtaatgcg tccgggatat cgccaatacg tgggccaaaa tcgataaacg
     5341 gcgtcacgtc ttgcagctct tccagccatt ccactgattt tccacaccgc tgaatgacat
     5401 aaaagtacgt tcgccatccg gctcaaccag cgccagacac cagccgttat cgccttcggc
     5461 gttatcgatc aggctgatta acccctcttt cgccatccgg ttgcgaataa tctccgccca
     5521 taccccctga ccgagcggca aggcattacc cgcttcgatg ccgaggcgct ttaacgccac
     5581 ggcaatattc agtgcgcagc cgccaacgtt aacgctctgc tgtttcagtt cgatatcgca
     5641 cccacgccag gggaggacat aagcgtcggc gatcacgtca atgaccgccg cgccgacgac
     5701 catcaccgac tgacgcgtgg ttaattcagg cagcagcgtg tgtaatcgag cgccgctcat
     5761 accgcctccc gttgttgacg atatttcgcc agcgctgtgg catagcggtt gaaatcaagc
     5821 tgatttaccg catccagctc cgcctttaac ggaggatcga tagcgttaat gccatgcaac
     5881 gcgccgcaga ttgccgtcgc catagcaccg atggtgtctg tatcgccgcc aaggttagcg
     5941 cagaggacgg cgcaacggtt cgggtcggtt tgtgccagtt caaccagcgc aatggcgcac
     6001 ggaacggact cgatagtgct ggtacctgcg ccaacgacct gatacagctg ttcgctggcg
     6061 gattcagtgc cgtcggcatt gcgcacaatt ttcagcgcaa tctccagacg tgctgccagt
     6121 gaggcgctga aggtggtgat gcgtttttgc tgggcatgtc gcgcaattga aggcagtgaa
     6181 tctacaatcg ctgaccagct ttctccgtca atggcacgag aaatcgccca tgcgatgact
     6241 accgcgcccg caaccgccag atcggattta tgggtcgggc tggacgccag cgccacatca
     6301 tcaataaagg aatcaacatc acgcgccgga agcaaacaac ctaatggcga gacgcgcatt
     6361 gccgcgccgt tggtcacgcc attgttttcc agttcagcaa cgggtttacc gtcgcgaatg
     6421 gcgttgagag caatcttcga ggtcggacct agtacgtttt tgttaaaggc gtcgaaacgc
     6481 agcgcccagt cgagaatatt acgcccaata agattcggat cgatcttgcc ttcacgttcc
     6541 agtaacgcat ccgccagaca cagcgccatc gaggtatcgt cggtgaattc ggcgcggtta
     6601 aaataacagg ccgcgttatt ctcctttggt ccaggaagaa aacggtcaat ccagccaaag
     6661 tgtgctttaa cgcggctgcg tggccaaagc tcggagggca tccccatcgc atcccctaac
     6721 gcctgcccat aaagagcacc gagaatacgt tctgttttca tttaacttcc ccttgtgtca
     6781 acgcaatatc gcgatcatcg accttgatag ccgtaatttc gttgtcggat tcgcggaaaa
     6841 aaatcatgaa cagcacggcg ataatggcaa tcatcaccgc gccgaaagtc cacatcccgg
     6901 accagttgaa agtcagtccg tttaccggtt cctgataagc gaacatcttt tccatcatca
     6961 caccgccaag acgatagccg agcaaactgc cgaagccctg gcagcagagc gtgatcagcc
     7021 cctgcgcagc ggtacgcata tgcacggggg cttttttatc gacatagatg taagcggtaa
     7081 cgtagtaaaa atcgtaactt acgccgtgaa gcaaaatacc gaggaacagt aacgcgtagg
     7141 tgaaatattc atccgcacta ccgtaaataa agaagccata gcggatcgca gcggtgacca
     7201 gaccaagcaa taataccttt ttgataccaa agcgtttagt gaaaaacggc aatgccagca
     7261 taaagaagat ttcagagaac tggccgagcg tcatccagcc ggtggcgttt ttcatgccaa
     7321 cttcggtcag ataaccgttg gcaaagatgt aatagaacgc cagtggcatc gcaaacagga
     7381 atgaacagaa gaaaaagacg aggaagtttt tatcgcgcag caggatcagc gcatccaggc
     7441 cgagcatgac tttaatatcc attttgccgg tgctttttgg tggcgtgtcg ggcaggaaaa
     7501 acgcaaacac accgagcaga gcagaacttc cggcggtaat cagcagcggg atgttagtcg
     7561 gtgagatatc ggcatacccc agtatttgcg gcaagaaacc acatgccaga ccggaggcaa
     7621 tccagccgat agtgcccatc acacgaatgc gcgggaaatc acgctcaaca tccggcacgt
     7681 tggcaaaagc gatgctgtta gtcagcgcaa tggtcggcat ataggttagc gagtaggcca
     7741 gcagtaacgg gaagaacccg gcaaaagtgg tctgttgcgc agcgaaatac atcagcagcg
     7801 cgcctgcgaa catcaatacc gccagcactt tctgcgccga gaaaaagcgg tcagtgatgg
     7861 agccaaccag aattggcgac aggatcgccg caatggcggt acaggcatac gaccagccaa
     7921 tttctccggc actaaaaccg cttttactta accacaacca caatggcaca aaccacgcgc
     7981 cccagataaa ccattcaaca aacatcatga acgacagctt tgctgttgtt ttcatctttt
     8041 aattccttca tgacaggtaa tgtaaggtac gcgatgacaa taccacttaa caataccttt
     8101 taaatacctt tgcagtgaaa acttgaccgc gctaacattt tttctgatga atcgagccaa
     8161 cagaaaacgc tgaaaaaaca tccaaaagat ggaaaaactc gcccgtaacg accaggatta
     8221 tgattgcccg caaaaggcgg gccaggaaaa tccatagccg atatccaatc ggaatttacg
     8281 ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac aaccttttac
     8341 agcaccgttg tatgactatt ccttctgacc agctttatct tcccggacat gactacgtag
     8401 accgcgtgat gattgacaat aatcgcccgc ctgcggtgtt acgtaatatg cagacgttgt
     8461 acaacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt gaccagggcg
     8521 ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac ccgaaaaaca
     8581 ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc gtcaacttac ggcgtgctgg
     8641 cgtcggtatc gcggcgttat gcgtatcgca ttccattcct cgtcaaactt aatcacaacg
     8701 agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg gagcaggcgt
     8761 tcaacatggg cgcggttgcg gttggtgcga ctatctattt tggctcggaa gagtcacgtc
     8821 gccagattga agaaatttct gcggcttttg aacgtgcgca cgagctgggc atggtgacag
     8881 tgctgtgggc ctatttgcgt aactccgcct ttaagaaaga tggcgttgat taccatgttt
     8941 ccgccgacct gactggtcag gcgaaccatc tggcggcgac cattggcgcg gacatcgtca
     9001 aacagaaaat ggcggaaaat aacggcggtt ataaagcgat taattacggt tataccgacg
     9061 atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt tatcagttag
     9121 ctaactgcta tatgggccgg gcagggttga taaactccgg cggtgctgcg ggcggtgaaa
     9181 ctgacctcag tgatgcagtg cgtactgcgg ttatcaacaa acgcgctggc ggaatggggc
     9241 tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa ctgattaacg
     9301 ccgtgcagga cgtttatctc gatagcaaaa ttactatcgc ctgatacgcc ttatacgaac
     9361 aacaccaccc cgtaggacgg ataaggcgtt tacgctgcat ccggcaacct atgcctgatg
     9421 cgacgctgaa gcgtcttatc atgcctacat agcactgcca cgtatgttta caccgcatcc
     9481 ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt tttttgcctt
     9541 tcactcctcg aacaattttc atattgtcgt ttttgtgatc gttatctcga tatttaaaaa
     9601 caaataattt cattatattt tgaaatcgaa aacaaacagc aggatatgaa aatgtacgtg
     9661 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
     9721 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
     9781 ccggtcatca tcgcaggaac gcctggcaca tttactcatg ctggtacaga aaatctgttg
     9841 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
     9901 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
     9961 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    10021 tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat
    10081 gatgtgcaaa tcaatgaagc cgatgctttt tataccaacc ccgctcaggc gcgtgaattt
    10141 gccgaggcaa ccggaattga ttccctggcg gtcgccatcg gcacggctca tggtatgtat
    10201 gccagcgcac cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta
    10261 ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg atattcagca aaccatcaaa
    10321 ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta
    10381 aaaaattacc tgaccgagca ccccgaagcg accgatcccc gggattattt gcagtcggct
    10441 aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg
    10501 gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa
    10561 agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc
    10621 ggcgctggca tttcatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca
    10681 ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt
    10741 tacgattgcc gataaagttg ggtttgcacg cgaacgcatt attctcggcg gcgatcatct
    10801 ggggccaaac tgctgacagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct
    10861 ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg
    10921 cgcgggggat cccataccgt tagcaccaga aacggttgcg gaacgagctg ctgtgctttg
    10981 ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag caactgagct atgtcattgg
    11041 caccgaagtt ccggttccgg gcggtgaggc cagcgccatt cagtcagtac acatcaccca
    11101 tgttgaagat gccgccaata ctttacgtac gcatcaaaag gcctttattg cccgtgggct
    11161 gacagaggcg ttaacacgtg tgattgccat cgtggtgcag ccgggtgtgg aatttgatca
    11221 cagcaatatt atccattatc agccgcagga agcgcaggcg ctggcgcaat ggatagaaaa
    11281 cacccgaatg gtttatgaag cacattctac cgattaccag acccggacgg cttattggga
    11341 attagtccgc gatcactttg caatattgaa agtcggtccc gcattaacct ttgctttacg
    11401 cgaggcgata tttgcactgg cacaaattga gcaggaactt atcgcccccg aaaatcgcag
    11461 cggttgcctg gcggtaattg aagaagtgat gctcgacgaa ccgcaatact ggaaaaaata
    11521 ttatcgtacg ggttttaacg attcattact ggatattcgt tacagcctgt cggatcgtat
    11581 tcgttattac tggccgcata gtcggattaa aaatagcgtc gaaacgatga tggtgaatct
    11641 tgaaggcgtg gacatcccac tgggcatgat tagtcagtat cttcccaaac aatttgaacg
    11701 cattcagtcc ggggaattat cagcaatacc gcatcagctg attatggata aaatttatga
    11761 tgttttgcgc gcctatcgct acggctgtgc ggaataagga cggtatatga ctaacctgtt
    11821 tgttcgtagc ggaatttatt ttgtcgatcg tagcgaagtt ttaacccata tcggtaatga
    11881 gatgctcgcc aaaggtgtgg ttcatgatac ctggccacag gcattaattg ccagagaagc
    11941 agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc attgtgaggc
    12001 gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag ttcattttca
    12061 gcaagcggat gatgataacg acgtggaggt atcgttggtt attgcgttga ttgtggaaaa
    12121 tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac agcccgatat
    12181 cgtcgagaca ctaatcactc ttcctgaaac ccagttaaag gaatacttca caaagtatgt
    12241 tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta tgaaacgcaa
    12301 gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg aagaaattaa
    12361 agagttgtgt cagaatcata atattcctgt tgaattaatc cagtgtcgg
//
LOCUS       DAJTMK010000122        12325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTMK010000122 DAJTMK010000000
VERSION     DAJTMK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12325
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.124"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..614
                     /locus_tag="OO135_002902"
     CDS             <1..614
                     /locus_tag="OO135_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5398675.1"
                     /translation="SILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMIA
                     LRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSW
                     IVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTELGSRIICRDKQLIF
                     EEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKSSGGKKG"
     gene            611..1225
                     /locus_tag="OO135_002903"
     CDS             611..1225
                     /locus_tag="OO135_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5398676.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1282..2739)
                     /gene="pepD"
                     /locus_tag="OO135_002904"
     CDS             complement(1282..2739)
                     /gene="pepD"
                     /locus_tag="OO135_002904"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5398677.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3000..3458
                     /gene="gpt"
                     /locus_tag="OO135_002905"
     CDS             3000..3458
                     /gene="gpt"
                     /locus_tag="OO135_002905"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5398678.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3550..4794
                     /gene="frsA"
                     /locus_tag="OO135_002906"
     CDS             3550..4794
                     /gene="frsA"
                     /locus_tag="OO135_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5398679.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4852..5253
                     /gene="crl"
                     /locus_tag="OO135_002907"
     CDS             4852..5253
                     /gene="crl"
                     /locus_tag="OO135_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5398680.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPALEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5292..6347)
                     /gene="phoE"
                     /locus_tag="OO135_002908"
     CDS             complement(5292..6347)
                     /gene="phoE"
                     /locus_tag="OO135_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5398681.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTGNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6635..7738
                     /gene="proB"
                     /locus_tag="OO135_002909"
     CDS             6635..7738
                     /gene="proB"
                     /locus_tag="OO135_002909"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5398682.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPGG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7750..9003
                     /gene="proA"
                     /locus_tag="OO135_002910"
     CDS             7750..9003
                     /gene="proA"
                     /locus_tag="OO135_002910"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5398683.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNVSTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9118..9193
                     /locus_tag="OO135_002911"
     tRNA            9118..9193
                     /locus_tag="OO135_002911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9151..9153,aa:Thr,seq:cgt)
     gene            9359..10573
                     /locus_tag="OO135_002912"
     CDS             9359..10573
                     /locus_tag="OO135_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5398684.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIIIGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSDQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDALEKE
                     ENVVHGSFGKRA"
     gene            10716..11597
                     /locus_tag="OO135_002913"
     CDS             10716..11597
                     /locus_tag="OO135_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398685.1"
                     /translation="MVRAKKQDLAWFDISNYEFLNDLTLPDLIQELEWRDFLLRHAKD
                     DTAIFKEEYDIKYERIFSGDPNLTILNEEEEEVEEFIRKVNDEAPSLRNEYDDLPSLS
                     SAMGVSPVTFSELAMYSFSSIDQGFFKRDEEDCYLKANAMLASVTGNLSNCSPNIILV
                     SIDLDDATDDEIITSLTHLLPLWRKELKVPEREHVAQKRIGLKTLQKLISNRVIPIVD
                     LLIWGEKSGKEVSNPMISALVFSDDPKDTQAIKESIKPFALESISERYTRLLQLYVNK
                     DREMSLIKISDLMSRDL"
     gene            11795..11992
                     /locus_tag="OO135_002914"
     CDS             11795..11992
                     /locus_tag="OO135_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5398686.1"
                     /translation="MSQSFIRLSEVQRRTGYSKVWIYRLIKEQRFPQSVKIGSRSIAF
                     IESEIEEWINQRIAESRGEVA"
     gene            11992..>12325
                     /locus_tag="OO135_002915"
     CDS             11992..>12325
                     /locus_tag="OO135_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398687.1"
                     /translation="MNKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITH"
BASE COUNT         3166 a         2900 c         3168 g         3091 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tctcgcatca tggtttgcct caaggcagtg
       61 acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg tgtattaatc
      121 gccagatgat cgctttgcgc ataatgcaac aagttaaggc cacgggaagt ccggttctgg
      181 atgtggcgca taactttgtt agcgcgtgtc aaatcggcga tcaacagggc tggttgcatc
      241 gtaaaggtgc cacgccggat gacaacggtc tggtgattat tcccggttca cgtggtgatt
      301 actcctggat tgtacagcct gttgcgaacg aaaaaacgct ccactcgctg gcgcatgggg
      361 cagggcgtaa atgggggcgc accgagtgta aatggcgtct ggcagcgaaa tacacggtga
      421 cgcaactctc gcggactgaa cttggcagtc ggataatttg tcgcgataaa caactcatct
      481 ttgaagaagc gccacaagcc tataaatcgg ctgaaagcgt ggtgcaatgt ctggtgcagg
      541 ccggtttaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa agcagtggag
      601 ggaaaaaagg atgatcttgc tacaactctc ctcagcgcag gggccggaag aatgttgtct
      661 cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag acgtcgcggt
      721 aacggtgttg gaaacagaaa cgggccgcta ctctgacacg ctgcgttcag ctctggtttc
      781 tctggatggc gataacgcat gggcattaag cgaaagctgg tgtggcacta ttcagtggat
      841 ttgtccgagt ccgtatcggc ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg
      901 ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc tgcgttcgtc
      961 ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc
     1021 atccggtatt agcgtgaagg ttcagtcaga gcgtagtcag catgctaaca agcggctggc
     1081 acgattgctg attgcctgga agctggagca gcagcaacag gaaaatagcg cggtgctgaa
     1141 atcgcagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgagac ggacatttac
     1201 agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc
     1261 atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag
     1321 tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt
     1381 gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca
     1441 ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc gctgataggt
     1501 ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc
     1561 tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta
     1621 gtctttaccg ctgtcgatca gtgaacggat caggcagtga atttctacgt tattgtcagt
     1681 catggtcacc acaccgacgt tcagggaggt ttcaaccaca cctttggcta catcggagtt
     1741 gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc
     1801 aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt
     1861 ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt tcagtgcgtc
     1921 gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag
     1981 tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac ccgccaggaa
     2041 gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc
     2101 gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg
     2161 atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc
     2221 ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc
     2281 aaaagcaccg tccataccgg cttcttcggt catggtcagc agcacttcca gcggaccgtg
     2341 aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc
     2401 acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc
     2461 tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg
     2521 taagacgacc ggtttacgat tttccatacc tgcggtagca ggtttacgaa tcaggatatt
     2581 acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc
     2641 agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc
     2701 ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca
     2761 cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg
     2821 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     2881 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     2941 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     3001 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     3061 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgg
     3121 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     3181 ccagctacga tcacgacaac cagcgagagc ttaaagtgct gaaacgcgca gaaggcgatg
     3241 gcgaaggctt catagttatt gatgacctgg tggataccgg tggtactgct gttgcgattc
     3301 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     3361 tggttgatga ctatgttgtt gatattccgc aagatacctg gatcgaacag ccgtgggata
     3421 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     3481 cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag tattctggag
     3541 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ttttaaacat
     3601 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     3661 gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     3721 tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     3781 gctgaacgta ccgacgatga tttatacgat actgtgattg gctaccgtgg tggcaactgg
     3841 atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat
     3901 ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat tgccgcctat
     3961 cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa ccgcgcctat
     4021 gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac cgtacccggc
     4081 ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt cccgacagta
     4141 ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta tgaacgttat
     4201 tttgcgccgc gcggcattgt gatgctgact attgatatgc cgtcggtggg cttttcttca
     4261 aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct
     4321 aacgtaccgt gggtggatca cactcgcgtc gctgcctttg gtttccgttt cggcgctaac
     4381 gttgccgtgc gtctggcata tcttgaatcg ccgcgtctga aagcggttgc ctgtcttggt
     4441 ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat
     4501 cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc gttacgcgtg
     4561 gagctgaatc gctattcatt aaaagtgcaa ggattgctgg gacgtcgctg cccaacgcca
     4621 atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc acgcttaatc
     4681 acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt gtatcggaat
     4741 tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg ttaaaaattt
     4801 gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc aatgacgtta
     4861 ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg cccgtatatt
     4921 cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt atgcgtcaac
     4981 gtgaaaccgg cactggaagt gcgtgaattc tggggctggt ggatggagct ggaagcgcag
     5041 gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg cgactggaag
     5101 agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct gcgtgagttt
     5161 cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc ggcggatgat
     5221 tttcgtgacg agccggtgaa gttaacggcg tgagtgaaat gtgccggatg catcacatcc
     5281 ggcaatatct attaaaactg atacgtcatc ccaatcgcga caatatcatc attattaata
     5341 ttcaatttgt tatcgctatc cagttggttg attttataat caacaaatgc tgacatattt
     5401 ttgttgaaat aatacgtagc accgacgtcg atataattaa ccagatcttc accaccgatg
     5461 ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag accaaagtca
     5521 aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc gccagttatt
     5581 ggcgtcattt tgcgagtttc agaatagaaa gttgccagat aaatattatt ggcatcgtat
     5641 ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg caggttctgc
     5701 tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct gccgccaaag
     5761 tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc gcggttttcg
     5821 tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa gaagtcggtg
     5881 ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgccggt ctgcgccgag
     5941 gagtcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata caacgcgccc
     6001 aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc aaaagcgaga
     6061 cgcgtttttt gctgtgcagt atcactctct gctttattac cggcaaactc tgcttcccaa
     6121 cgaccataac cagtcagttg atcgttaatt tgtgtttcgc ctttaaaacc aaaacggata
     6181 taactctggt cgccatcttt actgtcgtta tcactcatat aatgcatggc tttaactttg
     6241 ccatagacat ccagtttatt accgtctttg ttatatattt ctgcggcctg tacggatgca
     6301 gatgccacaa tgcccatcac cactaatgcc agagtgctct ttttcatttt cattcctgat
     6361 tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaaaaggaa agtttttaac
     6421 ctgagattgt taaagatata ttacagatta ataatattct taaaatgtgg taatttatta
     6481 aatctgtaat aaaagcgtaa acaactgccg ctaggcttgc tgatcccgcg caacaaaacg
     6541 ccatgctttg ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac gttcgtttga
     6601 tatcattttt cctaaaattg aatggcagag aatcatgagt gacagccaga cgctggtggt
     6661 aaaactcggc accagtgtgc taacaggcgg atcgcgccgt ctgaaccgtg cccatatcgt
     6721 tgaacttgtt cgccagtgcg cgcagttaca tgccgccggg catcggattg ttattgtgac
     6781 gtcgggcgcg atcgccgccg gacgtgagca cttgggttac ccggaactgc cagcgactat
     6841 cgcctcgaaa caactgctgg cagcggtagg gcagagtcga ctgattcaac tgtgggaaca
     6901 gctgttttcg atttatggca ttcacgtcgg gcaaatgctg ctgacccgtg ctgatatgga
     6961 agaccgtgaa cgcttcctga acgcccgcga caccctgcgt gcgttgctcg ataacaatat
     7021 cgttccggta atcaatgaga acgatgctgt cgctacggca gagattaaag tcggcgataa
     7081 cgataacctt tctgcgctgg cggcgattct ggcgggtgcc gataaactgt tgctgctgac
     7141 cgatcaaaaa ggtttgtata ccgctgaccc gcgcagcaat ccgcaggcag aactgattaa
     7201 agatgtttac ggcattgatg acgcactgcg cgcgattgcc ggtgacagcg tttcaggcct
     7261 cggaactggc ggcatgagta ccaaattgca ggccgctgac gtggcttgcc gtgcgggtat
     7321 cgacaccatt attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga tggaaggcat
     7381 ttccgtcggt acgctgttcc atgcccaggc gactccgctt gaaaaccgta aacgctggat
     7441 tttcggtgcg ccgccggggg gtgaaatcac ggtagatgaa ggggcaactg ccgccattct
     7501 ggaacgcggc agctccctgt tgccgaaagg cattaaaagc gtgactggca atttctcgcg
     7561 tggtgaagtc atccgcattt gcaacctcga aggtcgcgat atcgcccacg gtgtcagtcg
     7621 ttacaacagc gatgcattac gccgtattgc cggacaccac tcgcaagaaa ttgatgcaat
     7681 actgggatat gaatacggcc cggttgccgt tcaccgtgat gacatgatta cccgttaagg
     7741 agctggccga tgctggaaca aatgggcatt gccgcgaagc aagcctcgta taaattagcg
     7801 caactctcca gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga actggaagca
     7861 caaagcgaaa tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg agccaatggc
     7921 cttagcgaag cgatgcttga ccgtctggca ctgacgcccg cacggctgaa aggcattgcc
     7981 gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat cgatggcggc
     8041 gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt tattggcgtg
     8101 atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct gaaaaccggt
     8161 aatgcggtga tcctgcgcgg tggcaaagaa acgtgtcgca ctaacgctgc aacggtggcg
     8221 gtgattcagg acgccctgaa atcctgcggc ttaccggcgg gtgccgtgca ggcgattgat
     8281 aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat cgacatgctg
     8341 atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac aatcccggtg
     8401 atcacaggtg gtataggcgt atgccatatt tacgttgatg aaagtgtaga gatcgctgaa
     8461 gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa tacggttgaa
     8521 acgttgctgg tgaataaaaa catcgccgat agcttcctgc ccgcattaag caaacaaatg
     8581 gcggaaagcg gcgtgacatt acacgcagat gcagctgcac tggcgcagtt gcaggcaggc
     8641 cctgcgaagg tggttgctgt taaagccgaa gagtatgacg atgagtttct gtcattagat
     8701 ttgaacgtca aaatcgtcag cgatcttgac gatgccatcg cccatattcg tgaacacggc
     8761 acacaacact ccgatgcgat cctgacctgc gatatgcgca acgcccagcg ttttgttaac
     8821 gaagtggatt cgtccgctgt ttacgttaac gtctctacgc gtttcaccga cggcggccag
     8881 tttggtctgg gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg tggcccaatg
     8941 gggctggaag cactgaccac ttacaagtgg atcggcattg gtgattacac cattcgagcg
     9001 taaataaaac cgggtgatgt aaaagtagcc atttgattca caaggccatt gacgcatcgc
     9061 ccggttagtt ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc tttcagggcc
     9121 gatatagctc agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg ttcgactcct
     9181 attatcggca ccattcaaac atctccccaa gtctactaaa gtctttcaaa accccttata
     9241 atccgcgtat taaagccccg ttcgtctttt gacgcctact aaagtccctc aaaatctacg
     9301 gtcagatggg ggtacttatg ggggtatttg ctgttcggtt tagtggaggt acccccaagt
     9361 gaaactcaat gcccgtcaaa tagacacagc caagccaaaa gagaaggctt acaagctggc
     9421 cgatggtggc ggtttgtatc tcttggtaaa acctaatgga ggtaaatact ggcgacttaa
     9481 atatcgtgta gctggtaaag agaagctatt ggcactaggt gtgtatcctg aggttactct
     9541 agccgatgct cgggcaaaac gtgaagatgc gaaaagaggt atcgctggtg gtatcgatcc
     9601 gatggaagcg aaacgagagg aaaagattgc ccgggaaacg cagttaaaca acaccttcaa
     9661 agatattgcc cttgagtggc acagcagcaa attaaaaaaa tggtctgctg gttatgcttc
     9721 agacatcctc gaagccttca acaaagatgt gttcccttac attggcaaaa aaccaatcgc
     9781 cgaaatcaaa ccacttgaac ttctgaatgt gctgcggcgc atcgaggggc gcggtgctac
     9841 agaaaaagcc aaaaaagtga ggcagcggtg cggggaagtt ttccgctatg caatagtcac
     9901 tggtcgcgca gagtataacc ctgcaccaga ccttactagc gcgatgcaag gccatgaatc
     9961 taatcattac cctttcctta cagctaaaga attacctgat tttttcaagg cattgtccag
    10021 ttactcagga agcgcattgg ttgttatggc ggctcgtcta ctgattatca tcggcttgcg
    10081 gactggcgaa ctacgtggcg cattatggga tgaaattgat ttcaacaagg ctatctggga
    10141 gatacccgct tcacgtatga aaatgcggcg gcctcatatt gtgccattgt ctgatcaggc
    10201 tctttcgctt attgggaaga ttagagaaat aacaggcaat taccctctta tgtttcccgg
    10261 gcgcaatgat ccaaggaaaa caatgagtga ggccagcata aaccaagtgt ttaagcgcat
    10321 tggctacgct ggacgtgtaa ctggtcatgg gttccggcac actatgagca cgattttgca
    10381 tgagcagggc tataacaccg cgtggataga aacgcagctc gctcacgttg ataagaactc
    10441 aattcgtggc acatacaacc atgcgcaata tctggatgga aggcgggaga tgcttcaatg
    10501 gtatgccgac tatatggatg cgctggagaa ggaggagaac gttgttcatg gttcgtttgg
    10561 gaaacgagct taactgtatg aatagaccgt gttaagcaat ggtagtagac ttatgtagac
    10621 gaacaaagaa taggctatgt ctaggctgat ccccgaaaac ccgtacaccc ctacgggctg
    10681 gcatagccgt cagaattagg ggcgcggggt ggcgtatggt gagagccaaa aaacaagatt
    10741 tagcatggtt tgatattagt aattatgaat ttttaaatga tttaaccttg ccggacttga
    10801 ttcaagaatt agagtggcgc gattttttat tgcgtcatgc aaaagatgac actgctatat
    10861 tcaaagaaga gtacgatata aaatatgaaa ggattttttc gggcgatcct aacctaacca
    10921 ttctcaatga agaagaggaa gaggtcgaag aatttattcg caaagtaaat gatgaagccc
    10981 catcattaag aaatgagtac gatgatctac cctcattatc ttcagctatg ggggttagtc
    11041 cagtaacatt ttcagagctg gccatgtact ctttctcatc gatagatcaa ggctttttta
    11101 aaagagacga agaggattgc tacctcaagg caaacgctat gcttgcgagt gttactggaa
    11161 acctaagtaa ttgttcccct aacatcatac tcgtttctat agatttagat gacgccactg
    11221 atgacgaaat tattaccagc ctgactcacc ttctacctct atggcgaaaa gagttaaaag
    11281 taccagagag agagcatgtc gctcagaaaa ggattggatt aaagaccctt cagaagttga
    11341 tatctaatag agtgatacct attgttgatc tgctgatttg gggtgagaag tcaggtaagg
    11401 aagtgagcaa tccgatgatt tctgccttgg tttttagtga tgatccgaaa gatacgcagg
    11461 ctattaagga atcaataaag ccttttgcac ttgaatctat aagcgagaga tacacccgct
    11521 tgcttcagct ttatgtaaac aaagacagag aaatgagctt gataaaaata tctgatttaa
    11581 tgagtagaga tttatagcaa gcaaaatcct acgtaaacgt tttaagtaaa tttcgcgatt
    11641 tccgctttga atacgctact gtgtatttca aagcggataa tcttctcaaa aaaatatttt
    11701 atttttatcc gctatcaccc attcaccggt ttgtttaatc tcttttctgt ctactgatgt
    11761 ccattaaaga agatgacaga atataggagt aaacatgtct caatcattta ttcgtctttc
    11821 tgaggttcaa cgccgtactg gttacagcaa agtgtggatt tatcggctca tcaaagaaca
    11881 gcgattccct caatctgtaa agattggaag ccgatcaatt gcgtttattg aaagtgaaat
    11941 tgaagaatgg attaatcagc gcatcgctga gtcgcgtggc gaggtagcgt aatgaataag
    12001 aaaaaccgcc cattacaggc ggctaattca gatattcgcg tatctgatgt tacgcccctt
    12061 acaaaatccc ttcaagcacc aaagcgcaca ccgaaaaagc atcgtgccag agtctatatg
    12121 ctgcgtactg gtatagaggg atggacagaa aatgacattc ttcgctactg ccgtctgtct
    12181 tctggtcgta actatgcaac agagttagaa cgccagcttg gaatcactct ggaacgtatc
    12241 gacgaaaaga atcctgatgg tatcggaaca caccttcgct accgtttctc ctgccgtggt
    12301 gatgttctga aagtgatcac tcata
//
LOCUS       DAJTMK010000123        12140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTMK010000123 DAJTMK010000000
VERSION     DAJTMK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.125"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..309)
                     /locus_tag="OO135_002916"
     CDS             complement(<1..309)
                     /locus_tag="OO135_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCR5398688.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     V"
     gene            complement(424..822)
                     /locus_tag="OO135_002917"
     CDS             complement(424..822)
                     /locus_tag="OO135_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5398689.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1062..3476)
                     /gene="gyrB"
                     /locus_tag="OO135_002918"
     CDS             complement(1062..3476)
                     /gene="gyrB"
                     /locus_tag="OO135_002918"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5398690.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3505..4578)
                     /gene="recF"
                     /locus_tag="OO135_002919"
     CDS             complement(3505..4578)
                     /gene="recF"
                     /locus_tag="OO135_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5398691.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4578..5678)
                     /gene="dnaN"
                     /locus_tag="OO135_002920"
     CDS             complement(4578..5678)
                     /gene="dnaN"
                     /locus_tag="OO135_002920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5398692.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5683..7086)
                     /gene="dnaA"
                     /locus_tag="OO135_002921"
     CDS             complement(5683..7086)
                     /gene="dnaA"
                     /locus_tag="OO135_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5398693.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7383..7463
                     /locus_tag="OO135_002922"
     CDS             7383..7463
                     /locus_tag="OO135_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398694.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7693..7833
                     /gene="rpmH"
                     /locus_tag="OO135_002923"
     CDS             7693..7833
                     /gene="rpmH"
                     /locus_tag="OO135_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5398695.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7850..8209
                     /gene="rnpA"
                     /locus_tag="OO135_002924"
     CDS             7850..8209
                     /gene="rnpA"
                     /locus_tag="OO135_002924"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5398696.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8173..8430
                     /gene="yidD"
                     /locus_tag="OO135_002925"
     CDS             8173..8430
                     /gene="yidD"
                     /locus_tag="OO135_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5398697.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8433..10079
                     /gene="yidC"
                     /locus_tag="OO135_002926"
     CDS             8433..10079
                     /gene="yidC"
                     /locus_tag="OO135_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5398698.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10184..11548
                     /gene="mnmE"
                     /locus_tag="OO135_002927"
     CDS             10184..11548
                     /gene="mnmE"
                     /locus_tag="OO135_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5398699.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHVLIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11766..11870
                     /gene="tnaC"
                     /locus_tag="OO135_002928"
     CDS             11766..11870
                     /gene="tnaC"
                     /locus_tag="OO135_002928"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5398700.1"
                     /translation="MSFEDKCIMNILHICVTSKWFNIDNKGNAANLLI"
     gene            11899..>12140
                     /locus_tag="OO135_002929"
     CDS             11899..>12140
                     /locus_tag="OO135_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5398701.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTV"
BASE COUNT         2773 a         3321 c         3135 g         2911 t
ORIGIN      
        1 gacttcgcga gagagttttt ccaggaaacg atagtcgtca taactgagag cagtttgcgc
       61 cacggtgcta ccatcagcgg ccttctgtac cagcgcgccg ttataagtaa tgcagtagtc
      121 gcccggctgt tccatatgca gctctttcag gtagttgtga acgcctgcat aagggcgacc
      181 cgtcgttagc acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc
      241 gggtgaaatg gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt
      301 gatagccatg agttccccag gtagattagt tcctgaccca tgctaacgcg attccgctca
      361 aaaatcagta caacacccga gggaaaaggg ggatgcaacg cgggttgcat gctccccatt
      421 tgcttagcgg aagagtttcc ctttcagcag ttccatacct gcggaaagca gatcgttatt
      481 ggcttgtgaa gacacttcac cttgcggtga gagcgcatca ataatcttcg gcaattgttc
      541 tgccagtaaa ctggaagccg tactggtatc cacgccaagt ttttgcccga gatcggacac
      601 cgcatttgtg ccgagcgccg attccagttg ctcgccgcta actgattgat tgctctgctg
      661 attactcaac caggttgaga gaatggcccc taagccgcca ctttgcagtt tttccagcag
      721 cacctgaatg ccgccctgct cctcaaccca acttaaaata gcctgatatt tccccgcatc
      781 gcctttcaga aaggcaccga caacttcatc aaaaagcccc atgataatca cctgtaaagc
      841 gttacgtgtt gacccaaaaa gtatagatct gcgggatgat aattgcggat tgcagaaata
      901 aaaagggcgg agatgatctc cgcccttttt ttatagcttc ttgccggatg cggcgttaac
      961 gtcttacctg gcctacaaaa tcattaaaat tcaatacatt gcaagatttt cgtaggcctg
     1021 ataagcgtag cgcatcaggc atgctcgcat ggttaacgcc attaaatatc aatattcgcc
     1081 gctttcaggg cgttctcttc aataaacgca cgacgcggtt ctacggcgtc gcccatcagc
     1141 gtggtgaaca acaggtcagc ggcaatcgca tccttaacgg taacgcgcag catacgacga
     1201 ctttccgggt ccatggtggt ttcccacagc tgttccgggt tcatctcgcc cagaccttta
     1261 taacgctgga tggagagacc gcgacgagac tctttcacca gccagtccag cgcctgctcg
     1321 aagctggcta ccggctgacg acgctcgcca cgttcgataa atgcatcttc ttccagcaag
     1381 ccacgcagtt tctcacccag cgtgcagata cgacgatatt cgccaccagt gataaattcg
     1441 tgatccagcg gatagtcagt atccacaccg tgggtacgca cgcgaacaat cggctcgaac
     1501 aggttttgtt cggcattggt gtggacatca aacttccact ggctgccgtg ctgttctttg
     1561 tcgttcagtt cgctgaccag cgcgttcacc cagcgggtaa cggtctgctc atcagagagg
     1621 tcggcttccg tcagcgtcgg ctgatagata agctctttca gcattgcttt cggataacga
     1681 cgctccatgc ggttgatcat tttctgcgtc gcgttgtact cagacaccag tttctctaac
     1741 gcttcgccag ccaatgccgg tgcgctggcg ttggtgtgca gcgttgcgcc gtccagcgcg
     1801 atagagatct ggtactgatc catcgcttcg tcgtctttaa tgtactgttc ctgcttgcct
     1861 ttcttcactt tgtacagcgg cggctgagcg atgtagacgt gaccgcgttc aacgatttcc
     1921 ggcatctgac gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc gtcgacgtcc
     1981 gcatcggtca tgatgatgat gctgtgataa cgcagtttgt ccgggttgta ctcgtcacga
     2041 ccgataccac agccaagcgc ggtgataagc gtcgccactt cctgagaaga gagcatctta
     2101 tcgaagcgcg ctttctcaac gttgaggatt ttacccttca gcggcagaat cgcctggttc
     2161 ttgcggttac gcccctgctt cgcagagccg cccgcggagt ccccttccac caggtacagt
     2221 tcggaaagcg ccggatcgcg ttcctggcag tctgccagtt tgcccggcag gcccgctaag
     2281 tcgagcgcac ctttacggcg ggtcatttca cgcgcgcgac gcgccgcttc acgggcacgg
     2341 gcagcatcga taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc cagcaggtat
     2401 tctgccagca gttcgttcat ctgctgttca accgccgatt tcacctcaga agaaaccagt
     2461 ttgtctttgg tctgggagga gaatttcggg tccggcactt ttacggaaac gaccgcaatc
     2521 aggccttcac gcgcatcgtc accggtggcg ctgactttgg cttttttgct gtagccttct
     2581 ttgtccatgt aggcgttcag ggtacgggtc atcgccgcac ggaagcctgc caggtgggta
     2641 ccgccgtcac gctgcggaat gttgttggta aagcagtaga tgttttcctg gaagccatcg
     2701 ttccactgca acgccacttc gacgccaata ccgtcttttt cggtggagaa gtagaagata
     2761 ttcggatgga tcggcgtttt gttcttgttc agatattcaa cgaacgcctt gatgccgcct
     2821 tcatagtgga agtggtcttc tttgccgtca cgcttgtcgc gcagacgaat ggaaacgccg
     2881 gagttgagga acgacaactc acgcagacgt ttcgccagaa tttcatattc gaactcggtc
     2941 acattggtga aggtttcgag gctgggccag aaacgcacca tggtgccggt tttttcagtc
     3001 tcgccggtaa ccgccagcgg agcctgcggt acaccgtgtt cgtagatctg acggtgaatt
     3061 ttaccctcgc gctggataac cagctccagt ttttgcgaca gggcgtttac taccgaaaca
     3121 ccaacgccgt gcagaccgcc ggacacttta taggagttat cgtcaaattt accgcctgcg
     3181 tgcagaacgg tcatgatcac ttccgccgcc gatacgccct cttccgggtg aataccggtc
     3241 ggaatgccgc gcccgtcatc ctgtacagag acagagttat cggcgtgaat ggtgacgata
     3301 atttctttac agtgacccgc gagcgcttcg tcgatagcgt tatctaccac ctcgaatacc
     3361 atgtggtgca gaccggtgcc gtcatccgtg tcgccgatat acatacccgg gcgcttacgc
     3421 accgcatcca gccctttcag gactttgata ctggaggagt cataagaatt cgacatcaac
     3481 gtttctcgct catttatact tgggttaatc cgttatttta cccttttcca cggtaaacat
     3541 cttcgaattt tcgtccgaca tgtctataac gtgttcagca ctgatcgcgc tgacaaagac
     3601 ctgtgattgc gtcgctttta agcggctggc aagcagcccg cgacgctcat catcaagctc
     3661 agaggcaaaa tcatctatca ggtagagaca ccgccgcccg ctttcacggg tgaggaactc
     3721 tccttgcgcc agacgtaagg cgcacatcaa cagcttaagc tgcccacgcg ataaggtatc
     3781 ttccaccggc gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc catgcgcggt
     3841 gtaggttagc tggcgatcgc gttcaaaatt acgttccagc acctcagcat attctgtctc
     3901 tttctcccag ccgcgctgga aagagaaagt cagagaaaac tcagggagaa attgcttaca
     3961 agtatcggcc atatcagccg cgataccggc gctatactcc gcgcgccagg tgctgatttg
     4021 ctccgccagc gggatcagct ctttatccca cgggcgtagc tgttcgtaac gtgtcacctg
     4081 gcgcagcgcc gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg cggtgaaaaa
     4141 tccgggttcg ttgtgaaagc atgcccagtc gagaaatgct cttctgtatt tggggccgcc
     4201 gttgagtaaa gtaaaccctt ctggcgttat cagctgcatc ggcatcaggt gcgccagctc
     4261 cgcgacttta tgcccgtcag taccgtcgat gcggactttg ctgtcgcctt gtttgtcttt
     4321 ggttaagcca atcgccgtct cgcgctcttc gccctgtaat cgcccatgga gaacaaatgc
     4381 ctcctgctca tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc gaccatggcc
     4441 gagcgtatag atggcttcca gcacgctggt tttgccactg ccgttggcac ctaccagaaa
     4501 gttaaagccg ggagataagg cgagatccgc ggtttcgata ttgcggaaat cgcggatcaa
     4561 caagcgggtg agggacatta cagtctcatt ggcatgacaa cataagccgc gctctggctg
     4621 gccgcatctt caatctgcac gctggaaacc gaatcggtca gcatcatgcg gacgttttcg
     4681 catttcagcg cgttcagaac atccagcaca tagctgacgt tgaagccgat ttccatctcc
     4741 gcaccgctat aggtaacgtc gaggatctct tccgcttctt cctgttccgg gttgttggca
     4801 gtgattttca gctggttttc gctgacataa agacgtacgc cgcggaattt ctcgttagag
     4861 agaattgccg cacgggcaaa cgcctgcttg agcagatcgc agccagcttc cagatgtttg
     4921 tccgggttct tcggtagaac gcggcggtaa tccgggaagc gaccatccac cagtttggag
     4981 gtgaagataa agtcgccaac gtgggcacga atgttattgc tgccaatctg cacgcgcagc
     5041 ggattgtcgc cgccgtcgag catacgcatc agttcaatca cgcctttacg cggcacgatc
     5101 accgaatggc ttggcaaaga ttgaccaatt ggcattgaac agactgccag acggtgacca
     5161 tcggtcgcta cggtgcgcag ttcttcgcct tcggtttcaa acagcatacc gtttaagtaa
     5221 tagcgaacat cctgatgcgc catcgaaaac tgggtcgctt caatcagacg cttcatcgtc
     5281 gcctgcggca gggtaaattc gacttcgctc tgccagtcat cgaggttcgg gaaatccgcc
     5341 gctggcaggg tagacagcga aaaacggcta cgcccggagc gcaccagcat ccgttcacct
     5401 tccagctgca cggcaatttc cgcgccttca ggcagaccac ggcagatatc aaagaatttg
     5461 cgcgccggaa cggtcgttgc tcctggctcg tgtggctgaa ccagcgcaac acgtgccacc
     5521 atttccatct cgagatcagt accggtcagc gacaacgtac cgtcagcaac ctgtaacagc
     5581 agattaccga gaatcggtag cgtaggacga ccacctaacg gaccgctcac ctgttgtagc
     5641 ggttttaata aatgctcacg ttctacggta aatttcatag gtttacgatg acaatgttct
     5701 gattaaattt gaaaaatctt ctttgatatc gtggctctct tcacgcaact gctcgatctt
     5761 acggcaggca tgaagcaccg tcgtgtggtc acgaccacca aacgcatcgc caatctccgg
     5821 cagactgtgg ttagtcagct ctttcgccag cgccatcgcc atctggcgcg gacgcgccac
     5881 cgagcgggat cgacgcttgg aaaggagatc cgcgactttg atcttgtagt actccgccac
     5941 cgtcttctga atattgtcga tggtgaccag tttttcctgc aatgccagca agtcgcgcag
     6001 cgcctcacgc acgaagtcga tggtgatcgc ccgtccggta aagttggcat tggcaatgac
     6061 gcggttcagc gccccttcca gctcacgtac gttagatcgt agacgcttgg cgataaagaa
     6121 cgccacttcg cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca ggatcgccac
     6181 acgggtttcc agctctggcg gttcgatcgc cacagtcagt ccccaaccga agcgggattt
     6241 caaacgatcc tcaacgccgt tgatctcttt cggatagcga tccgaggtga gaatgatctg
     6301 ttgattacct tccagcaggg cgttgaaggt atggaaaaac tcttcctgag atcgttcttt
     6361 attagcaaaa aactgaatat cgtcgatcag cagtgcatct acggaacggt agtagcgttt
     6421 aaactcttcg atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa agcgctcgga
     6481 gtgcatataa accactttgg cattcggctt gcgcgccata atgccgttac ccaccgcatg
     6541 cagcaggtga gttttaccca gacccgtgcc gccataaagg aacaacgggt tataggcacc
     6601 gccagggtta tccgccacct ggcgagccgc cgcgcgcgcc agttggttag atttaccttc
     6661 aacgaagtta tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg gttctgccgg
     6721 ggccgggacg ttatcccaac ctgagcgcgt agaaggcgca gcacgttgcg gctgcgtttg
     6781 cgccacctgt gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg tttgcgtcac
     6841 cggtttggtg ccgacttcaa aacgcagctg tggggcatcc gctccgcaga aactggttag
     6901 cagtccattg atattattaa ggtacttgtc ccgtacccaa tcgaggacaa aacggtttgg
     6961 cgcgtacagg gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta tccacatact
     7021 gaattctgtg gctggtaact catcctgcaa tcgggcaaga cactgctgcc aaagcgaaag
     7081 tgacacggcg gactccactc gaacaaaagt cgataatgac taaggctgaa acattcatga
     7141 ttgttgacgt acgtcgaaaa gaccctgctt gagggtgacg cacgaaccgc tgtctgcggt
     7201 tatatgcccg atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc ctaaactgcg
     7261 caagagatct tctgtttctc acagattttc ccgatttatc cacaggactt tccagaactc
     7321 gctaagtgta aacgatcctg ccgcgaggcg ggcacgattt acgccgcata ttgaaaaatt
     7381 taatgaccat agacaaaaat tggcttaatc gatctaataa agatccagga cgatccttgc
     7441 gctttaccca tcagcccgta taatcctcca cccggcgcgc catgctggtt tccactggtg
     7501 tgaggtcgta cattttccct gcgaaaaggt gcggaaaagc gcggtaaata aggaaagaga
     7561 attgactccg gagtgtacaa ttattacaat ccggcctctt taatcaccca tggcttcggt
     7621 gtccatcgtt tcatttttcg gcggatatcc aataaagcca ttgaatttat tcaagtttag
     7681 gtagaaatcg ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa ccgttctcac
     7741 ggcttccgtg ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg tcgtcgtgct
     7801 aaaggccgcg ctcgtctgac cgtttctaag taataaagct aacccctgag tggttaagct
     7861 cgcatttccc agggagttac gcttgttaac tcccagtcaa ttcacattcg tcttccagca
     7921 gccacaacgg gctggcacgc cgcaaattac cattctcggc cgcctgaatt cgctggggca
     7981 tccccgtatc ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg aacgcaatcg
     8041 gattaaacgt ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc cggctatgga
     8101 tttcgtggtg gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc tctcggaagc
     8161 gttggaaaaa ttatggcgcc gccactgtcg cctggctcgc gggtcctgat agccctcatt
     8221 cgggtctatc aacgcctgat tagtccgcta ctcgggccgc attgtcgttt cactccaacc
     8281 tgttcaagct acggaattga ggcattgcgc aggtttggag tgataaaagg cagttggttg
     8341 acggtgaaac gcgtattaaa atgccaccct ttacaccctg gtggtgacga tcccgtcccg
     8401 cccggaccat ttgataccag agaacactaa cgatggattc gcaacgcaat cttttagtca
     8461 tcgctttgct gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat aaaaacccgc
     8521 aacctcaggc ccaacagacc acgcagacaa cgaccaccgc agcgggtagc gccgccgacc
     8581 agggcgtacc ggccagtggc caggggaaac tgatctcggt taagaccgac gtgcttgatc
     8641 tgaccatcaa cacccgtggt ggtgatgttg agcaagctct gctgcctgct tacccgaaag
     8701 agctgaactc tacccagccg ttccagctgc tggaaacttc accgcagttt atttatcagg
     8761 cacagagcgg tctgaccggt cgtgatggcc cggataaccc ggctaacggc ccgcgtccgc
     8821 tgtataacgt tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa ctgcaggtgc
     8881 cgatgacgta taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc ctgaaacgtg
     8941 gtgattacgc tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa ccgctggaaa
     9001 tctccacctt tggtcagttg aagcaatcca tcactctgcc accgcatctc gataccggaa
     9061 gcagcaactt cgcactgcac accttccgcg gcgcggcgta ctccacgcct gacgagaagt
     9121 acgagaaata caagttcgat accattgccg ataacgaaaa cctgaacatc tcttcgaaag
     9181 gtggttgggt ggcaatgctg caacagtatt tcgcgaccgc gtggatcccg cataacgacg
     9241 gtaccaacaa cttctatacc gctaatctgg gtaacggcat cgccgctatc ggctataaat
     9301 ctcagccggt actggttcag cctggtcaga ctggcgcgat gaacagcacc ctgtgggttg
     9361 gcccggaaat ccaggacaaa atggcagctg ttgctccgca cctggatctg accgttgatt
     9421 acggttggtt gtggttcatc tctcagccgc tgttcaaact gctgaaatgg atccatagct
     9481 ttgtgggtaa ctggggcttc tccattatca tcatcacctt tatcgttcgt ggcatcatgt
     9541 acccgctgac caaaacgcag tacacctcca tggcgaagat gcgtatgctg cagccgaaga
     9601 ttcaggcaat gcgtgagcgt ctgggcgatg acaaacagcg tatcagccag gaaatgatgg
     9661 cgctgtacaa agctgagaag gttaacccgc tgggcggctg cttcccgctg ctgatccaga
     9721 tgccaatctt cctggcgttg tactacatgc tgatgggttc cgttgaactg cgtcaggcac
     9781 cgtttgcact gtggatccac gacctgtcgg cacaggaccc gtactacatc ctgccgatcc
     9841 tgatgggcgt aacgatgttc ttcattcaga agatgtcgcc gaccacagtg accgacccga
     9901 tgcagcagaa gatcatgacc tttatgccgg tcatcttcac cgtgttcttc ctgtggttcc
     9961 cgtcaggtct ggtgctgtac tatatcgtca gcaacctggt aaccattatt cagcagcagc
    10021 tgatttaccg tggtctggaa aaacgtggcc tgcatagccg cgagaagaaa aaatcctgat
    10081 tcggtgagtt ttcgctaaaa taagggcggt cagttgaccg cttttttctt ttcgtagggc
    10141 ggataagcac cgcgcatccg ccacacaaag caacaggaac atcatgagcg ataatgacac
    10201 tatcgtagcc caggccacgc ctccgggacg tggcggcgtt ggcatcctgc gcatctccgg
    10261 cttcaaagcc cgtgaagttg ccgaaaccgt gctgggtaaa ctgcctaagc cgcgctacgc
    10321 cgattatctt ccgtttaaag acgccgacgg cagcgtgctc gatcagggga ttgcgctatg
    10381 gttccctggc ccgaactcgt tcaccggcga agatgtgctg gaactgcaag gtcatggcgg
    10441 tccggtgatc ctcgacctgc tgttaaaacg cattctgacc attcccggcc tgcggattgc
    10501 tcgccctggt gagttttccg aacgcgcgtt tcttaacgat aaacttgact tagcccaggc
    10561 cgaggcgatt gccgatctta tcgacgccag ttcggaacag gcggcccgtt cggcacttaa
    10621 ctcgctgcaa ggcgcattct ccgcacgggt taatcatctg gtagaagccc tcacccactt
    10681 gcgcatttac gtcgaagcgg caattgattt ccccgatgaa gagatcgatt tcctctccga
    10741 cggaaaaatt gaagcccagc tcaatgacgt tattgccgat cttgatgcag tgcgtgctga
    10801 agcacgtcag ggtagtttgt tgcgcgaagg gatgaaagtg gtgattgccg gacgtcctaa
    10861 cgccggtaaa tcgagcctgt taaacgcgct ggcggggcgt gaagcggcaa tcgtaaccga
    10921 tatcgccgga actacgcgtg acgtgctgcg tgagcatatc cacattgacg gaatgccgct
    10981 gcatatcatc gataccgccg ggctacgtga agccagtgac gaagtagaac gtattggtat
    11041 cgagcgcgcg tggcaggaaa ttgaacaggc cgaccgcgtg ctgtttatgg tcgatggcac
    11101 cacaacagac gccgtggatc cggcagagat ctggccggaa tttattgccc gtctgccagc
    11161 gaaactgccg atcaccgtgg tgcgcaataa agccgatatc accggcgaaa cgctgggaat
    11221 gagtgaagtg aacggtcacg tgttaattcg tctctcggca aggactggtg aaggcgtgga
    11281 cgtgctgcgt aaccatctca aacagagcat gggctttgac accaacatgg aaggcggctt
    11341 cctggcgcgt cgtcgccacc tacaggcgct ggaacaggca gcggaacatc tacaacaggg
    11401 caaagcgcaa ctgttgggag cctgggcagg tgaactgctg gcggaagagt tgcgtctggc
    11461 acagcagaac ttaagcgaaa tcaccgggga atttacttca gacgacctgc tggggcggat
    11521 tttctccagt ttctgtattg gtaagtaacc gcgcttacga agccgtattc tgactgtcag
    11581 atgcggcttc gcttcattgt tagcactcct gttattcctc aacccttttt ttaaacatta
    11641 aaattcttac gtaatttata atcttaaaaa aagcatttaa tattgctccc cgaacgattg
    11701 tgattcgatt cacatttaaa caattttaga atagacaaaa ttcttgagta ttatactgta
    11761 gcctcgtgtc tttcgaggat aagtgcatta tgaatatctt acatatatgt gtgacctcaa
    11821 aatggttcaa tatcgacaat aagggtaatg ctgccaactt actgatttag tgtatgatgg
    11881 tgattttaag gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact
    11941 gaaggcgtgg tgcgtaacgg caaaagcact gccggacatc agcgctatct ctgctctcat
    12001 tgccgtaaaa catggcaact acagttcact tacaccgcct ctcagcccgg tacgcaccag
    12061 aaaatcattg atatggccat gaatggcgtc ggatgtcgcg ccagtgcacg cattatgggc
    12121 gttggcctca acacggtttt
//
LOCUS       DAJTMK010000124        12106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTMK010000124 DAJTMK010000000
VERSION     DAJTMK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.126"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..266)
                     /locus_tag="OO135_002930"
                     /pseudo
     CDS             complement(1..266)
                     /locus_tag="OO135_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; frameshifted;
                     incomplete; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            455..985
                     /gene="cybB"
                     /locus_tag="OO135_002931"
     CDS             455..985
                     /gene="cybB"
                     /locus_tag="OO135_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5398702.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1230..1403
                     /locus_tag="OO135_002932"
     CDS             1230..1403
                     /locus_tag="OO135_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398703.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(1475..1624)
                     /gene="hokB"
                     /locus_tag="OO135_002933"
     CDS             complement(1475..1624)
                     /gene="hokB"
                     /locus_tag="OO135_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5398704.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2023..3663
                     /gene="trg"
                     /locus_tag="OO135_002934"
     CDS             2023..3663
                     /gene="trg"
                     /locus_tag="OO135_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5398705.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDESAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(3701..4630)
                     /locus_tag="OO135_002935"
     CDS             complement(3701..4630)
                     /locus_tag="OO135_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5398706.1"
                     /translation="MAYGKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPAL
                     SKTLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNN
                     DVVRIGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRM
                     SDPELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSD
                     ALVQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGH
                     GAGEPIGILTRVDAMFSSGCQLMINAIRKSMPF"
     gene            4840..6183
                     /locus_tag="OO135_002936"
     CDS             4840..6183
                     /locus_tag="OO135_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5398707.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILQRQTSFRALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            6408..8063
                     /gene="opgD"
                     /locus_tag="OO135_002937"
                     /gene_synonym="mdoD"
     CDS             6408..8063
                     /gene="opgD"
                     /locus_tag="OO135_002937"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5398708.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8203..8427
                     /locus_tag="OO135_002938"
     CDS             8203..8427
                     /locus_tag="OO135_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5398709.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            8490..9026
                     /gene="rimL"
                     /locus_tag="OO135_002939"
     CDS             8490..9026
                     /gene="rimL"
                     /locus_tag="OO135_002939"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5398710.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQIALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9021..10000)
                     /locus_tag="OO135_002940"
                     /pseudo
     CDS             complement(9021..10000)
                     /locus_tag="OO135_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10124..11116
                     /gene="tehA"
                     /locus_tag="OO135_002941"
     CDS             10124..11116
                     /gene="tehA"
                     /locus_tag="OO135_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5398711.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11113..11706
                     /gene="tehB"
                     /locus_tag="OO135_002942"
     CDS             11113..11706
                     /gene="tehB"
                     /locus_tag="OO135_002942"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5398712.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            12009..>12106
                     /locus_tag="OO135_002943"
     CDS             12009..>12106
                     /locus_tag="OO135_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5398713.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSG"
BASE COUNT         3112 a         2803 c         3029 g         3162 t
ORIGIN      
        1 ctttcgcttt ccacggaata tttttagcct ctttttcggc gtaaaccgcg atacttttcc
       61 catcaacgat aagtgaatct tccgtaaaat caacgctcca ggggaatggt ccgtagtttg
      121 aatcatgttt cagcaggtag gcgagaattt ttggggaagt gagatcatta atagcgacaa
      181 cgtctatgtt tgcttttgac ttcaagtaat cgacgcaaca ccagtcgacc gatacgacca
      241 aaaccgttaa taccaacctt actcatggtt ttctcctgtc aggaacgttc ggatgaaaat
      301 tgatcctttc caggcttagc ccaggattgt gggatgggca atctccattc ttacagtgaa
      361 acgtaacgta ctgaaaacgg gtgaacaata tttaatgaaa ttttgagaaa agcccgttat
      421 gttaacggaa cattatgtta aagcaggaaa tgttatggaa aataaatact caaggttaca
      481 aatcagcatt cactggctgg tctttttgct ggttatcgca gcgtattgcg caatggagtt
      541 tcggggtttc ttcccacgta gcgatcggcc actcatcaac atgattcatg tttcctgcgg
      601 catctcaatt ctcgtgctga tggtcgttcg tcttttgtta aggctgaaat acccaacccc
      661 gccgattata cctaagccaa aaccgatgat gacgggactg gcgcatttgg gacatttggt
      721 gatttatctc ctgtttattg cgctgccagt gattggtttg gtgatgatgt ataaccgggg
      781 caacccgtgg tttgcgtttg gtttgacgat gccttacgct tcagaggcca atttcgaacg
      841 ggttgatagc ttaaagtcgt ggcatgaaac gctggcgaat ctgggatatt ttgtcatcgg
      901 gttgcacgct gcggctgcac tggcacacca ctatttctgg aaggacaaca cacttctacg
      961 catgatgccg cgtaaacgtt cctgaaggat atttaaagaa aacgcctgta ctaaaaccga
     1021 cccgtggtac aggcgaagaa tacgggtcta catcggaagc gcctattata tttatttgta
     1081 tgacaaataa aaccataatc cttgcccata cgtccatctg gcttattttt aatcaattca
     1141 cccgatcttt gatctcatca acggtatcaa aataaaccag cgtataacgt tcaaatcgaa
     1201 cataagattc gataaaccat ggaggttata tgaaaaaact ggcacttatt ttgtttatgg
     1261 gaacgcttgt ttccttttat gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg
     1321 ggatctcaca ctgtacggca ggcggcaaat ttgtctgtaa tgatggttct attagtgcct
     1381 cgaaaaaaac atgcactaac tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta
     1441 gtcagatgcg cggggaaaat ccccgcgctt gcccttacct ggacgtgcag gccatgagcg
     1501 cagcaacctc cttatcaccg tcccggaacc gcagttcgta gagcgtttgt cgggtcagga
     1561 gtgtgaatgt cagaatcgta atgcagataa tgagcagaca caccaccaga gggttgtgct
     1621 tcatagcctt tctccttgcc ggatggcggg taagaggcta agatctgaat tgctaggttc
     1681 attcgttggc ctcggttgat agaaatatcg gtcggggcct tcgtctttct gattcccggt
     1741 tagcctgaaa acagaaagtc tcaggcaccc gcaggcatcc tatgaggttt ccttagggac
     1801 gaaaataatc acttcacgaa attgcgtgct gttttccaga atttttcgtc attcggatta
     1861 gccagtttag ccattcgtta ctctcttcat tccaatagca ttaattttct ctgcaataat
     1921 tgttgtaaaa atgtgacgca aagaggtttt tggtcataag taattaccgt caagtgccga
     1981 tgactttcta tcaggagtaa acctggacga gagacaacgg taatgaatac aactccctca
     2041 cagcgattag gttttttgca tcacatcagg ctggttccgt tatttgcctg cattcttggc
     2101 ggtatcttag ttctattcgc attaagttct gctctggctg gctatttcct ctggcaggcc
     2161 gatcgcgatc agcgtgatgt tactgcggag attgagattc ggaccgggtt agcgaacagt
     2221 tcagattttt tgcgttcagc ccggatcaat atgattcagg ccggggctgc gagtcgtatt
     2281 gcggaaatgg aagcaatgaa gcgaaatatt gcgcaagccg aatcggagat taaacagtcg
     2341 cagcaaggtt atcgtgctta tcagaatcga ccggtgaaaa cacctgctga tgaagccctc
     2401 gacactgaat taaatcaacg ctttcaggct tatatcacgg gtatgcaacc tatgttgaaa
     2461 tatgccaaaa atggcatgtt tgaagcgatt atcaatcatg aaagtgagca gatccgaccg
     2521 ctggataatg cttataccga tattttgaac aaagccgtta agatacgtag caccagagcc
     2581 aaccaactgg cggaactggc ccatcagcgc acccgcctgg gtgggatgtt catgattggc
     2641 gcgtttgtgc ttgccctggt catgacgctg ataacattta tggtgctacg tcggatcgtc
     2701 attcgtccac tgcaacatgc cgcacaacgg attgaaaaaa tcgccagtgg cgatctgacg
     2761 atgaatgatg aatcggcggg tcgtaatgaa atcggtcgct taagtcgtca tttacagcaa
     2821 atgcagcatt cactggggat gacagtaggg actgttcgac agggcgcgga agagatttat
     2881 cgtggcacca gcgaaatttc agctggcaat gcggacctgt catctcgcac cgaagaacaa
     2941 gcggcggcta tcgaacaaac tgccgccagc atggagcaac tcactgcgac ggtgaaacag
     3001 aatgcggata acgcgcatca tgccagcaaa ctggcgcaag aggcttctat taaagccagc
     3061 gatggcgggc agacggtttc cggtgtagta aaaacgatgg gcgctatctc cacgagttcg
     3121 aagaaaattt ctgagatcac cgccgtcatc aacagtattg ctttccagac gaatattctg
     3181 gcactgaatg ctgccgttga agccgcgcga gcgggtgagc aagggcgtgg atttgccgtt
     3241 gtcgccagcg aagtacggac actcgcaagt cgcagcgctc aggcggcgaa agagattgaa
     3301 ggcttgatca gtgaatcagt caggttaatt gacctggggt cggatgaggt ggcaacggcc
     3361 gggaaaacca tgagcactat tgttgatgcc gtcgcgagtg tcacacatat catgcaggaa
     3421 atcgccgccg cctcggatga acaaagtaga ggcataacgc aggttagcca ggcgatttct
     3481 gaaatggata aggtgacgca acagaatgct tctctggtag aagaggcctc agcggcggcg
     3541 gtgtcccttg aagaacaggc ggcacgatta actgaggcgg tggatgtatt ccgtctgaac
     3601 aaacattctg tgtcggcaga acctcgcgga gcgggtgaac cagttagttt cgctacggtg
     3661 tgaaaatgtt caaggaggga tcgacagatc ccttcacctt tcagaacggc attgattttc
     3721 gaatagcgtt aatcatcaac tggcaaccag aagagaacat cgcatctacg cgggtcagta
     3781 ttccaatcgg ttcgcctgca ccatgtcccg gaacaggcag ggccaccagc gtggcatgac
     3841 gcaggtcgtc ttttaccgcg ccagaaggga caaaccacac gtagtcgtat tcaaccgtaa
     3901 gttgacgaga tagcgaagca gacagcgttt cgatacaacc cgaaggaatt ttacatccct
     3961 ggctctgtac taatgcatct gaatgctggc gtggcgcagt gccttctggt gatacaacga
     4021 ccggccattc cagcactcgg cttagcgtta cgttctcctg aagtagcggg tgattagggc
     4081 ggacaaccag cttcagcgat tcaagaaaca gcagttcgta attaagcccg gtcatcagtt
     4141 caggatctga catccgacca atgccgatat cgatttcccc ggttttcaaa cccgccagaa
     4201 tcatagggtt actcattgtc gcaacttgca aggtcgtctc tttttgttgc tgatgaaact
     4261 gacctataac cgaaggtaat atccccagtg ccgcagtagg tagtgcacca atcctgacga
     4321 catcattatt aagaccttct ttacgatgaa gcgactgtcc ggcagtgttg atggcgtcaa
     4381 gaactctgac tgcatgcgtt aaaaattgtt cgccgggtaa ggtaagttgc gccccctgac
     4441 gaccacgctc aaacaagcga gcgcccgtca gctgctccag ttcattcaat gtcttagaga
     4501 gcgcaggttg actcaaatta agggtttcag ccgcgcgccc caaagttcct tgttgtgcga
     4561 cagctacgaa tgtatgaagg tggcgcaaac ggatgcgctg actaaacaga ctattttttc
     4621 cataagcgat gttaaaaacg aagcggtgtc gctgacaagt gaagttgttt gattatgata
     4681 acttgattgc aaaatataat taacaattaa agcaattgtg ttacagcaaa atggataata
     4741 ttgatgtttt cgcggcgaga tcacagtttg taaattcttc ccgccagagt gaatgcggtt
     4801 acctacactc cagattactg accactggag gcagacacta tggcgaacag catcacggcg
     4861 gatgagattc gggaacagtt ttcgcaggca atgtcagcca tgtaccagca agaagttccg
     4921 cagtacggca cgctgctgga actggtagct gatgtgaatc tggctgtgct ggaaaacaat
     4981 cctcaactgc acgaaaaaat ggtaaatgca gacgagctgg cgcgactgaa tgttgaacgt
     5041 catggggcga ttcgcgttgg gactgcacaa gagcttgcta ctcttcggcg gatgtttgcc
     5101 attatgggga tgtacccggt gagctattac gatctctcgc aggcaggggt gccggtacat
     5161 tcgacagcat ttcggcccat tgatgatgct tctctggcgc gtaatccctt ccgcgttttt
     5221 acctccttac tccgccttga gcttatcgag aacgaaattt tgcgccagaa agcggcggag
     5281 attctgcgtc agcgcgatat cttcacccca cgttgtcgac aactgttaga ggaatatgag
     5341 cagcagggcg gttttaacga aacacaggca caggaatttg tgcaagaggc cctggaaacg
     5401 tttcgctggc accagtcagc aacggtagat gaagaaacct atcgcgcctt gcacaacgaa
     5461 catcggttga ttgctgatgt ggtctgtttt cctggatgcc atatcaacca cctgacgcca
     5521 cgtacgctgg atattgaccg ggtgcagtcg atgatgcctg aatgcggaat tgaacccaaa
     5581 attctcatcg aggggccgcc gcgccgcgag gtaccgattt tacaacgcca gaccagcttt
     5641 agagcactgg aagagacggt gttgtttgcg gggcagaaac agggcacgca taccgcgcgc
     5701 tttggtgaaa ttgagcagcg tggcgtggca ttaacgccga aagggcgaca actgtatgat
     5761 gatcttctgc gtaacgctgg aaccgggcag gataatctca cacaccaaat gcatttacag
     5821 gaaaccttcc gcacttttcc tgacagtgag tttttaatgc gtcagcaagg gttggcatgg
     5881 ttccggtacc gtctgacgcc ttcgggtgag gcgcatcgtc aggcgattca tcttggagac
     5941 gatccacagc ctttaattga acgtggttgg gtagtggcgc aacccatcac ctatgaagat
     6001 ttcttgcccg ttagcgcggc ggggatcttc cagtcaaatc tgggtaatga aacgcagaca
     6061 cgcagtcacg gtaatgccag tcgcgaagca tttgagcagg cgttgggttg tccggttttg
     6121 gatgagttcc agctttacca ggaagcggaa gaacgcagta aacgtcgctg tggtttgctt
     6181 taaattctga ccatccgcct ttgcaaaaat ttgcctgatt ttacaaacga atcaggctca
     6241 tcccatcgac ataaaaaaaa tgccgattta tgcatattct ctcagttcaa caattggatt
     6301 attaataaat attgtctaga gtgagcggtc ataaataagc actttcttgc cgctgaaaac
     6361 gaccagcgcg ggaccattca caacaccaga aggactcact ttcaggtatg gatcgtagac
     6421 gatttattaa aggttcaatg gctatggccg ccgtgtgcgg taccagcggc attgcttctc
     6481 ttttttctca ggcggcattc gcggcagatt ctgatattgc cgacgggcaa acccagcgtt
     6541 ttgacttctc cattctacag tcaatggcgc acgacttagc gcaaacagcg tggcgtggtg
     6601 cgcctcgtcc gttacctgac acgctggcga caatgacgcc gcaggcttat aacagtattc
     6661 aatacgacgc cgaaaaatcg ctctggcata acgttgagaa ccgtcaactg gacgctcagt
     6721 tcttccatat gggaatggga ttccgtcgcc gcgttcgtat gttttctgta gatccagcaa
     6781 cacatctggc gcgtgaaatt cactttcgcc cggagttgtt caaatacaac gatgcgggtg
     6841 ttgatacaaa acaattagaa gggcaaagcg atctcggctt tgccggtttt cgcgtgttta
     6901 aagcccccga actggcgcgc cgtgatgtag tatcatttct cggcgcgagt tatttccgcg
     6961 ccgttgatga tacatatcaa tacggtttgt cggcccgcgg cctggcgatc gacacttaca
     7021 ccgacagtaa agaagagttc cccgacttta ccgccttctg gtttgatacg gtaaaaccgg
     7081 gggcaactac ctttaccgtt tatgcgttgc tcgatagcgc cagcattact ggtgcctata
     7141 agttcactat ccattgtgag aaaagtcagg tgattatgga tgtggaaaat cacctgtatg
     7201 cgcgcaaaga cattaaacag ctgggcattg cgccgatgac cagtatgttc agctgcggta
     7261 ctaatgaacg tcggatgtgc gatacaattc atccgcaaat tcatgactct gatcgtctgt
     7321 ccatgtggcg gggcaacggc gagtggattt gccgtccgct gaataatccg caaaaattgc
     7381 agttcaatgc ttacaccgac aacaacccga aagggtttgg tttattgcaa ctggatcgtg
     7441 acttctccca ttatcaggac attatgggct ggtataacaa acgcccaagt ctgtgggtgg
     7501 aaccgcgtaa caagtggggt aagggcacca tcggcctgat ggaaatccca acaacgggcg
     7561 aaacgctgga taacattgtc tgtttctggc agccagaaaa agctgtaaaa gcaggtgatg
     7621 agtttgcatt ccagtatcgt ctgtactgga gtgcgcaacc gcctgttcat tgcccattag
     7681 cgcgcgttat ggcgacgcgt accggcatgg gcggtttccc ggaaggttgg gcgccaggtg
     7741 aacactatcc cgaaaaatgg gcgcgtcgtt ttgccgtcga tttcgttggt ggtgatctga
     7801 aagctgccgc gccaaaaggc attgagccgg tgattacgct ttccagtggg gaagcgaagc
     7861 aaatcgaaat tctctatatt gaacccatcg atggttatcg tattcagttt gactggtatc
     7921 cgacttcgga ctccactgat ccggtcgata tgcggatgta tctacgttgt cagggggacg
     7981 ctatcagtga aacatggctg tatcagtatt tcccgccagc gccggataaa cgtcagtatg
     8041 ttgacgaccg cgtgatgagt taatcgtttt ttcttcggca ccttcttcgg gaggtgccgt
     8101 ctggttaaac acgatcccgc tcgcattttt ccctaagtta aatgagtaat ctgatggtgt
     8161 gtatttcaga tacatcttgt cagccactaa cagggagtgc gtatgtttcc agaataccga
     8221 gatttaatat cccgtctgaa aaacgaaaat cctcgcttta tgtccttgtt cgataaacac
     8281 aataaacttg atcatgaaat tgccagaaag gaaggtttcg acggtcgagg gtacaatgcg
     8341 gaagtggtcc gcatgaaaaa acaaaagcta cagttaaaag atgagatgct caaaatcctg
     8401 cagcaggaga gcgtcaaagg ggtgtaaact ttcctaagcc gccagccagg cggctttttt
     8461 aacaacagca tggattgact ggagataaga tgactgaaac gataaaagta agcgaatcac
     8521 ttgaattaca tgctgttgcg gaaagtcacg tcacacctct ttatcagtta atctgtaaaa
     8581 ataaaacctg gttacagcag tcgctaaact ggccgcagtt cgttcagagt gaagaggaca
     8641 cgcgaaaaac ggtgcagggt aatgtgatgt tgcatcaacg cggctatgcc aaaatgttca
     8701 tgattttcaa agaagatgaa cttatcggcg ttatctcgtt taatcgtatt gaaccactga
     8761 ataaaactgc tgaaatcggc tactggctgg acgaatctca tcaggggcag gggatcattt
     8821 ctcaggcgct gcaggcattg attcatcatt acgctcagtc tggtgaactt agacgcttcg
     8881 tgatcaaatg tcgggtggac aatccgcaaa gcaaccagat cgctttgcgc aatggtttta
     8941 tccttgaagg ttgcctgaaa caggctgagt tcctgaatga tgcctatgat gatgtgaact
     9001 tatacgcgcg tattatcgat tcataataac cctgccagcg gcgttcgcgt aatgcgctcc
     9061 tcgccgttaa tgacttttgg accacggaca tgaaccgtgt cgccatcaaa ggcttcaacg
     9121 acggcgtggt cggttaactc cacatggttt tcaattatca ccgcacctga aatacggctc
     9181 tcaccttgta ttacaacgtg ctcgtcgagc agaatcggtt caccacgtac cacggcattt
     9241 ccgccaatca agacatgatg ttttaacaca caattacctt ccacaatggc atattccgcc
     9301 acctgcgaac tgtaatgaat cgtcggaatg gcatcttctt ctatgccagc tttcacctgc
     9361 gcgtgaccgt agactttagc gcaatcgcat agccagatgt tgttctcttc attaccttca
     9421 acactggcaa aatcaaaaac ttcggcgcga tgttcaataa aagcataccg gacgacggca
     9481 tcgccataaa tttgtgcctg gtggacaata cgcgaggcgc taacccttgc gcgatcataa
     9541 atttggagca ggagttgatg gtcgggcgtt aagccttgtg cggcgacgat catagaatgt
     9601 tgatcaatta aggcgtgacc aaatattcta cattgcccgt aaactaatga gtcatgaatg
     9661 gtgacactgt cacttatata tgcaccctga ctgatttcac tattatcgat ccagacgtta
     9721 tccgttgcgt aaacctctcc ccacagcacg ctggtgccgg tgatacgcgt attaccagaa
     9781 atcaccgttc cgccgaacgc aatagcattc tggtcgtaaa tccagcaatt tccttcctat
     9841 gctaacaccg tttcgcgatc gatccagccg ccagcggttc ctgctatcac atcgttaaaa
     9901 tcgctaatgg cgataatctg ccgtaataac acactttttt agtgccatct tcctgataac
     9961 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    10021 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    10081 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    10141 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    10201 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    10261 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    10321 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    10381 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    10441 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    10501 attatggcgc ggatctcacc ctgaagaagc taccatgcct ggactgtatc tgccgacagt
    10561 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    10621 ggtgttttta ggcgcaggcg ttttctcatg gctaagcctt gaaccggtga tcttgcagcg
    10681 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    10741 tcctgcgctg gtggcctgta gtgcttggtt gagcgtcaac ggcggcgagg gtgatacgct
    10801 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    10861 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    10921 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    10981 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    11041 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgctgttt taatgaaagc
    11101 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    11161 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    11221 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    11281 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    11341 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    11401 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    11461 attgccaata tgcaacgttg cactaaactt ggtggttaca acctgattgt ggcggcgatg
    11521 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    11581 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    11641 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    11701 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    11761 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    11821 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    11881 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    11941 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catctcagga
    12001 aactattcat gcgtaccaca tcatttgcga aagttgcagc tttatgcggc ttgttggctc
    12061 tgtctggttg tgcctctaaa atcacccagc cagataaata ttctgg
//
LOCUS       DAJTMK010000125        12040 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTMK010000125 DAJTMK010000000
VERSION     DAJTMK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12040
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.127"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..413)
                     /locus_tag="OO135_002944"
     CDS             complement(<1..413)
                     /locus_tag="OO135_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5398714.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSVG"
     gene            569..1609
                     /gene="gpr"
                     /locus_tag="OO135_002945"
     CDS             569..1609
                     /gene="gpr"
                     /locus_tag="OO135_002945"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5398715.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1649..2143)
                     /locus_tag="OO135_002946"
     CDS             complement(1649..2143)
                     /locus_tag="OO135_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5398716.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2334..3218
                     /gene="yghA"
                     /locus_tag="OO135_002947"
     CDS             2334..3218
                     /gene="yghA"
                     /locus_tag="OO135_002947"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5398717.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVTGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="OO135_002948"
     CDS             complement(3490..3915)
                     /gene="exbD"
                     /locus_tag="OO135_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5398718.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="OO135_002949"
     CDS             complement(3922..4656)
                     /gene="exbB"
                     /locus_tag="OO135_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5398719.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4908..6095
                     /gene="metC"
                     /locus_tag="OO135_002950"
     CDS             4908..6095
                     /gene="metC"
                     /locus_tag="OO135_002950"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5398720.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6235..6894
                     /locus_tag="OO135_002951"
     CDS             6235..6894
                     /locus_tag="OO135_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5398721.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTITILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="OO135_002952"
     CDS             complement(6934..7833)
                     /gene="yqhC"
                     /locus_tag="OO135_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5398722.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8027..9190
                     /gene="yqhD"
                     /locus_tag="OO135_002953"
     CDS             8027..9190
                     /gene="yqhD"
                     /locus_tag="OO135_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5398723.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPNALKKPENYDVRANVMWAATQALNCLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9295..10122
                     /gene="dkgA"
                     /locus_tag="OO135_002954"
                     /pseudo
     CDS             9295..10122
                     /gene="dkgA"
                     /locus_tag="OO135_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            10322..11248
                     /locus_tag="OO135_002955"
     CDS             10322..11248
                     /locus_tag="OO135_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5398724.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11299..11556
                     /gene="yqhH"
                     /locus_tag="OO135_002956"
     CDS             11299..11556
                     /gene="yqhH"
                     /locus_tag="OO135_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5398725.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            11635..>12040
                     /locus_tag="OO135_002957"
     CDS             11635..>12040
                     /locus_tag="OO135_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398726.1"
                     /translation="MRRWHTCFFILLFCGFSGNVLCKESINEAIERLYQPLTSNDDIP
                     ITDEFSLDLRSNMSATPGKMTPWLNNNLLCRCVDDAFVSAEIIQSTHLNGGRTHVLLQ
                     LVIKQNRTDIHRLLGLMLVRENDNHRWLIEDID"
BASE COUNT         3115 a         3061 c         2999 g         2865 t
ORIGIN      
        1 ccaacggaac ttaagccgtc aggtgccagg gcgatatacc ccgccttcgt cactcgccgt
       61 gccacatctt cgatatacgg attcagtcca cgattctcat gcaccaccac cacggctggc
      121 gttttgccgc tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt
      181 ggcgaaggat acgtgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc
      241 gtcagcgcat aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc
      301 gcatacttcg ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg agcgtaatag
      361 tcgtagtaat ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca
      421 ttcctcaact ggtgtttttt aagcaaagca taagcgcgtg tttttgccca gtttttcgtc
      481 actctgtgag ccagactacg ggatacgcgc tggcgaatcg ctaaactaga aacattgttt
      541 cgaaattgaa cggtggaaag gagaggtcat ggtctggtta gcgaatcccg aacgttacgg
      601 gcagatgcag taccgctatt gcggaaaaag tggtttacgc ctgcccgcgt tatcgctcgg
      661 tttatggcac aatttcggtc acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa
      721 agcgtttgat ttaggcatta cgcactttga tttagccaac aattacgggc cgcctccagg
      781 aagcgcagaa gagaactttg gtcgcctgct gcgggaggat tttgccgctt atcgcgatga
      841 actgattatc tctaccaagg ctggctacga tatgtggccc ggcccttacg gctctggcgg
      901 ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta
      961 tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc
     1021 tgcgctggct catgcggtac aaagcggtaa ggcgctgtat gtcgggatct cctcttactc
     1081 gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag tggaaaattc cgcttttaat
     1141 tcatcaacct tcgtacaatt tactgaaccg ctgggtggat aaaagcggcc tgctggatac
     1201 cctgcaaaat aacggcgtgg gctgtattgc ctttactcct ctggctcagg gattgctgac
     1261 cggaaaatat ctcaacggca ttccgcaaga ttcacggatg catcgtgaag ggaataaagt
     1321 tcgtggtctg acgccgaaaa tgcttaccga agccaacctc aacagcctgc gcttattgaa
     1381 tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa
     1441 agatgatcgc gtgacgtcgg tattgattgg tgtcagccgc gcggagcaac tagaggagaa
     1501 cgtgcaggcg ctgaataatc tgacatttag caccgaggag ctggcgcaga ttgatcagca
     1561 tatcgccgat ggcgagctga atctgtggca ggcgtcttcc gataaatgac ctgttaataa
     1621 cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg acgagtcagt cggtcaagat
     1681 agcccatcac aaatgcagag agcacaaacg tcagatggat aatgacgtac cacatcagtt
     1741 tgttatccgg gacatttttc gcatccataa agacgcgcag taagtgaatg gaagaaattg
     1801 ccacaatcga cgctgctact ttgtttttca gcgacgttgc gtccattttc cccagccagt
     1861 tcagcttctc tttgttctcg gagatatcca gctgcgagac gaaattctca taaccggaaa
     1921 acatcaccat caccagcaaa ccgccaacca gcgtcatatc caccagcgac agcaacacga
     1981 ggatcaaatc tgattccgcc atcgagaaga tattcggcag tacgtgaata atctcctgga
     2041 agaacttcag cgccagggca actaacgcca gcgaaaggcc aaagtacacg ggggcaagca
     2101 gccagcgaga agcatacatt gcattttcaa gaaaacgttc catacattcc tgtcgtgtag
     2161 ttaaaacggg caacagtata tcgcaattga gtaaacagca cgcaacagca aatcaacata
     2221 tacgttaaac ctttgtcgca taattctgag gtttagggat cttcctggtt cccttatcaa
     2281 cgcatcgaga gcgtctaccc ttaaaagacg ttgaacctga agggagaaaa acaatgtctc
     2341 atttaaaaga cccgactacg cagtattaca ctggtgaata tcccaaacag aaacaaccga
     2401 cgccaggcat ccaagcgaag atgacaccgg taccggattg cggcgagaaa acctatgttg
     2461 gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc
     2521 gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg
     2581 tggaagaaga agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg
     2641 ttctgctgcc aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc
     2701 acaaggcgtt aggcgggctg gatattatgg cgctggtcac cgggaaacag gttgccattc
     2761 cggatattgc agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg
     2821 cgctgttctg gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca
     2881 ccacttcgtc aatccaggca taccagccaa gtccgcattt actggactat gcggctacga
     2941 aggcggcgat tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc
     3001 gggtgaatat tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa
     3061 cgcaggataa gatcccgcag tttggtcagc aaacgccgat gaagcgtgcg gggcaaccgg
     3121 cggaactggc ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag
     3181 aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag
     3241 caaccgggca tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct
     3301 acaaacgcat gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc
     3361 atcaggcatt gagcacctaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaacg
     3421 cacgaagatt caacatgttg caatagattt tgtaggcctg atgagcgtag cgcatcaggc
     3481 aattctactt tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc
     3541 cgcctgatgc agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttatccgc
     3601 tcggaagaag atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat
     3661 tgtttcatcg gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag
     3721 ataaaccggt ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt
     3781 cacatctacc gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac
     3841 gtcgataaac ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt
     3901 aagatgcatt gccatcggat attatcctgc gcgtaatttt tgtgcgacac gaaccggatg
     3961 cgcagcggcg ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc
     4021 aacatcaccc agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac
     4081 gaccgcagga atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc
     4141 cggcgcaacg actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat
     4201 gatgccccag accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag
     4261 atagccgtta ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa
     4321 gctggtacgt tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt
     4381 ctgcgcttca ttgaacaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc
     4441 gttggcctgg tttaaggaac gcgcttcagc cagcagttgc tgctcgcgct ttaggcgacg
     4501 cttctgattg aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc
     4561 caaaataagc ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca
     4621 gacggaaagg tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca
     4681 caaaatctgc gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat
     4741 ttgcatactg ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca
     4801 gcacgccatt tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata
     4861 ttcatgctag tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa
     4921 agcttgatac tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa
     4981 atagcgtgat tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg
     5041 cgacgcgcaa tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt
     5101 tttccttaca acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct
     5161 gcggggcggc agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt
     5221 tgatgaccaa cactgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac
     5281 tgggcgtaac gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc
     5341 agccaaacac taaaatcgtg tttctggaat cgccaggctc catcaccatg gaagtccacg
     5401 acgttccggc gattgttgcc gccgtacgca gtgtggtgcc ggatgccatc attatgatcg
     5461 acaacacctg ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta
     5521 ttcaagccgc caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt
     5581 gcaatgcccg ttgctgggag cagctacggg aaaacgccta tctgatgggc cagatggtcg
     5641 atgccgatac cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc
     5701 aacatcatga aagcagtctg aaagtggctg aatggctggc agaacatccg caagtagcgc
     5761 gagttaacca ccctgctctg cctggcagta aaggacacga attctggaaa cgagacttta
     5821 caggtagcag cgggctgttt tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg
     5881 cgaactatct ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat
     5941 cgttgatcct ggcgaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg
     6001 attttagcgg gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg
     6061 ccgatctgga cgccggtttt gcgcgaattg tataacattg ccacttttgg acaattttgc
     6121 agacatttaa ttgtgaaaag tcttaaattg ttgcgtcccg gatcaaggcg tcccggacga
     6181 atcaggagta caataggcag ataaaggctt aaacgctgtt ccacaggaaa gtccatggct
     6241 gttattcaag atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct
     6301 catattgtta gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg
     6361 ctgcccgcct catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc
     6421 cagggggtta tggattttct gcctacgatt acgattctga ccgccgcagc aagtctgggc
     6481 tgctggctaa gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg
     6541 ctggcacagc ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt
     6601 ctgctggcgc tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg
     6661 gcgggaattt ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg
     6721 ctgtgggtca gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa
     6781 cgccatgaag atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct
     6841 ggcttgttag gcatgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt
     6901 tccccgttcc cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct
     6961 tgccgcatct tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga
     7021 tgcgctttca tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat
     7081 catcatccgc gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt
     7141 gacagactta aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg
     7201 ctcgacgctc aggttttcgg tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag
     7261 actgaagtga gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag
     7321 cacgtagtac agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg
     7381 ctccatcacg tcgagtaacc gctccgccgc gcataaaatc tcttctgata acgtggcgga
     7441 gttaatcccg ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa
     7501 cagttcctgt aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga
     7561 cgtggcatag gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc
     7621 atcataacga aacacgcgtt cattgatata accgatttta tgcccggaaa agagaattat
     7681 gatgccaggc tcgtacatca ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg
     7741 cacatcgggc aacagttctg acaaactatt ttctttattt ttcagtttat taactttatc
     7801 cgccagcaag cggcaaatct cttcacgttt catatcgcgt aatttcttag gaataatgcg
     7861 gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa ataggcaaga
     7921 cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa aaacatcagg
     7981 cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga acaactttaa
     8041 tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca
     8101 aattcctcac gatgctcgcg tattgattac ctacggtggc ggcagcgtga aaaaaaccgg
     8161 cgttctcgat caagttctgg atgccctgaa aggcatggac gtgctggaat ttggcggtat
     8221 tgagccaaac ccggcttatg aaacgctgat gaacgccgtg aaactggttc gcgaacagaa
     8281 agtgactttc ctgctggcgg ttggcggcgg ttctgtactg gacggcacca aatttatcgc
     8341 cgcagcggct aactatccgg aaaatatcga tccgtggcac attctgcaaa cgggcggtaa
     8401 agagattaaa agcgccatcc cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga
     8461 atccaacgca ggcgcggtga tctcccgtaa aaccactggc gacaagcagg cgttccattc
     8521 tgcccatgtt cagccggtat ttgccgtgct cgatccggtt tatacctaca ccctgccgcc
     8581 gcgtcaggtg gctaacggcg tagtggacgc ctttgtacac accgtggaac agtatgttac
     8641 caaaccggtt gatgccaaaa ttcaggaccg tttcgcagaa ggcattttgc tgacgctgat
     8701 cgaagatggt ccgaacgccc tgaaaaagcc agaaaactac gatgtgcgcg ccaacgtcat
     8761 gtgggcggca acgcaggcgc tgaactgttt gattggcgct ggcgtaccgc aggactgggc
     8821 aacgcatatg ctgggccacg aactgactgc gatgcacggt ctggatcacg cgcaaacact
     8881 ggctatcgtc ctgcctgcac tgtggaatga aaaacgcgat accaagcgcg ctaagctgct
     8941 gcaatatgct gaacgcgtct ggaacatcac tgaaggttcc gacgatgagc gtattgacgc
     9001 cgcgattgcc gcaacccgca atttctttga gcaattaggc gtgccgaccc acctctccga
     9061 ctacggtctg gacggcagct ccatcccggc tttgctgaaa aaactggaag agcacggcat
     9121 gacccaactg ggcgaaaatc atgacattac gttggatgtc agccgccgta tatacgaagc
     9181 cgcccgctaa gctttttacg cctcaaactt tcgttttcgg gcatttcgtc cagacttaag
     9241 ttcacaacac ctcaccggag cctgctccgg tgaggtcata taaaggagga acgtatggct
     9301 aatccaaccg ttattaagct acaggatggc aatgtcatgc cccagctggg actgggcgtc
     9361 tggcaagcaa gtaatgagga agtaatcacc gccattcaaa aagcgttaga agtgggttat
     9421 cgctcgattg ataccgccgc ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa
     9481 aatgcctcag tcaacagaga agaactgttc atcaccacta agctgtggaa cgacgaccac
     9541 aagcgccccc gcgaagccct gctcgacagc ctgaaaaaac tccagcttga ttatatcgac
     9601 ctctacttaa tgcactggcc cgttcccgct atcgaccatt atgtcgaagc atggaaaggc
     9661 atgatcgaat tgcaaaaaga gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc
     9721 catcacctgc aacgcctgat tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa
     9781 cttcatccgc tgatgcaaca acgccagcta cacgcctgga acgcgacaca caaaatccag
     9841 accgaatcct agagcccatt agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt
     9901 cgcgatctgg cagataaata cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg
     9961 gatagcggcc tggtggtgat cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt
    10021 gatgtctggg atttccgtct cgacaaagac gaactcggcg aaattgcaaa actcgatcag
    10081 ggcaagcgtc tcggtcccga tcctgaccag ttcggcggct aacatgcaaa ttctcccggt
    10141 ggcggtaatg ttccgctacc ggacttttca gaaatcattt attcccctcg cgtcccgccc
    10201 gttgttactc ttccttgttc aggaatgcca aatataagga catcatcatg cagagccgga
    10261 agctcttaaa agaacaactc atctatatcc gggataaacg caacggagag gtgaaaaaca
    10321 gatgaaaata atacttctgt ttttagcagc cctggcaagt tttaccgtac acgcgcagcc
    10381 tccttcacag accgtagaac aaacagtccg gcagatttat cagaactata aatcagatgc
    10441 cagtacgcct tatttcggtg aaaccggaga gcgggcgata acttcagcgc gtattcaaca
    10501 ggcgcttacc ctgaacgaca atcttacgct gccgggcaat attggctggc tggattatga
    10561 tccggtttgt gattgtcagg attttggcga tctggtgcta gaaagcgttg cgataaccca
    10621 aactgacgcc gatcatgccg atgccgttgt gcgctttcgt atctttaaag atgataaaga
    10681 aaagaccacg cagacactga aaatggtggc ggaaaatggt cgttgggtca ttgacgatat
    10741 tgtcagcaat catggcagcg tcttacaagc agttaatagc gagaatgaaa aaacgctggc
    10801 cgctttagct tcgttgcaaa aagaacagcc ggaagccttt gttgccgaac tctttgaaca
    10861 tattgctgat tatagctggc cgtggacgtg ggtggtttcc gactcttacc gccaggcggt
    10921 taatgccttc tataaaacca ccttcaagac ggccaataat cccgatgaag atatgcaaat
    10981 agaacggcaa tttatttacg acaatccgat ctgttttggc gaagagtcgc tattttcacg
    11041 cgttgatgaa attcgagtcc tggagaaaac cgccgattcc gcccgcattc atgttcgttt
    11101 tacgctgacc aatggcaaca acgaagagca agaactggtt ttacagcggc gcgaaggcaa
    11161 gtgggaaatc gctgatttta tccgcccgaa cagcggcagc ctacttaagc agattgaggc
    11221 aaaaactgcc gccagattaa agcaatgagc tgaattaaat aacaattagc cggaacaata
    11281 aataaaaggg aacactatat gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc
    11341 tgcttgctca gtggctgcgt caatgagcaa aaggtcaatc agctggcgag caatgtgcaa
    11401 acattaaatg ccaaaatcgc ccggcttgag caggatatga aagcactacg cccacaaatc
    11461 tatgctgcca aatccgaagc taacagagcc aatacgcgtc ttgatgcaca ggactatttt
    11521 gattgcctgc gctgcttgcg tatgtacgca gaatgataaa aaaatccccg gcagcatgtc
    11581 agttgccggg ttataggctg tttgctaaaa aaagccaaac ataaggacat gattatgcga
    11641 cgctggcaca cctgtttttt tattctgctg ttctgtggct tcagcggaaa cgtgttgtgc
    11701 aaagaatcga ttaatgaagc gatagaacgt ttgtaccaac cattgaccag taatgacgat
    11761 ataccaataa ccgatgaatt cagtctcgat ttgcgaagta atatgagcgc tactcctggc
    11821 aaaatgacac cctggctgaa taataatctt ttgtgtcgct gtgtcgatga cgctttcgtt
    11881 tctgcagaaa taatccaaag cacacaccta aatggcggcc gaacgcatgt actgttgcag
    11941 ctggttatca aacaaaatcg cacagatatc caccggttac tggggctaat gctggttcgt
    12001 gaaaatgata atcatcgttg gctaatagaa gacatcgatg
//
LOCUS       DAJTMK010000126        11874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTMK010000126 DAJTMK010000000
VERSION     DAJTMK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.128"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            216..380
                     /locus_tag="OO135_002958"
     CDS             216..380
                     /locus_tag="OO135_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5398727.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            383..550
                     /locus_tag="OO135_002959"
     CDS             383..550
                     /locus_tag="OO135_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398728.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            664..759
                     /locus_tag="OO135_002960"
     CDS             664..759
                     /locus_tag="OO135_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398729.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            923..3598
                     /gene="acnA"
                     /locus_tag="OO135_002961"
     CDS             923..3598
                     /gene="acnA"
                     /locus_tag="OO135_002961"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5398730.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGSGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWTAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGKEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3662..4252)
                     /gene="ribA"
                     /locus_tag="OO135_002962"
     CDS             complement(3662..4252)
                     /gene="ribA"
                     /locus_tag="OO135_002962"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5398731.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4422..5186
                     /gene="pgpB"
                     /locus_tag="OO135_002963"
     CDS             4422..5186
                     /gene="pgpB"
                     /locus_tag="OO135_002963"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5398732.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5335..5643
                     /gene="lapA"
                     /locus_tag="OO135_002964"
     CDS             5335..5643
                     /gene="lapA"
                     /locus_tag="OO135_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5398733.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5650..6819
                     /gene="lapB"
                     /locus_tag="OO135_002965"
     CDS             5650..6819
                     /gene="lapB"
                     /locus_tag="OO135_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5398734.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            7012..7749
                     /gene="pyrF"
                     /locus_tag="OO135_002966"
     CDS             7012..7749
                     /gene="pyrF"
                     /locus_tag="OO135_002966"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5398735.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7749..8075
                     /gene="yciH"
                     /locus_tag="OO135_002967"
     CDS             7749..8075
                     /gene="yciH"
                     /locus_tag="OO135_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5398736.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8201..8419)
                     /gene="osmB"
                     /locus_tag="OO135_002968"
     CDS             complement(8201..8419)
                     /gene="osmB"
                     /locus_tag="OO135_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5398737.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8688..9437)
                     /locus_tag="OO135_002969"
     CDS             complement(8688..9437)
                     /locus_tag="OO135_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5398738.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(9527..9700)
                     /locus_tag="OO135_002970"
     CDS             complement(9527..9700)
                     /locus_tag="OO135_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398739.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(9847..11796)
                     /gene="pdeR"
                     /locus_tag="OO135_002971"
                     /pseudo
     CDS             complement(9847..11796)
                     /gene="pdeR"
                     /locus_tag="OO135_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150331.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         3017 a         2800 c         3016 g         3041 t
ORIGIN      
        1 tgcacaatat ttttatccct catatttata gggtaaatta cctgtaaagt agtgtctaat
       61 tacccgcctc gcaaattcca gttttcaaat atctatttca ggtcaaaaga ttgaattatt
      121 cactggagac gattcgtaaa ttcgctggat ttttgactaa agtttcttta ggatttgtct
      181 catcgtatga ttaattacac caatcgttca acgtcttgct gataagtgat ggcgattata
      241 tgaggttagc aatgccttct ggaaatcagg aaccccgccg cgatcctgaa ttaaaacgta
      301 aagcctggct ggcggttttt cttggttctg cacttttctg ggtggttgtc gcactgctga
      361 tttggaaagt gtggggataa ctatggtcgg tcaggagcaa ctggagtcgt caccattatg
      421 ccagcatagt gacaatgaaa cagaaacgaa acgggaatgt tccgtcgtta ttccagacga
      481 ctggcaacta acatcgcagc agcaagcctt tatagaactg tttgctgaag atgatcagcc
      541 gaaacaataa ttatcatcat tcttattacc catttttaat gaattaaagg gcttttaata
      601 caccgcagca ataacagctt gagttatctc aacacaaaat aataaccgtt aagggtgtag
      661 gctatgatca acacaaatat gaaatattgg tcctggatgg gcgcgttttc tctgtcgatg
      721 ctcttctggg ccgaactcct ctggatcatt actcactgat ccttgacccc gctgcggcgg
      781 ggttgtcatt tgctttgcca caaggtttct cctcttttat caatttgggt tgttatcaaa
      841 tcgttacgcg atgtttgtgt tatctttaat attcaccctg aagagaatca gggcttcgca
      901 accctgtcat taaggaggag ctatgtcgtc aaccctacga gaagccagta aggacacgtt
      961 gcaggccaaa gataaaactt accactacta cagcctgccg cttgctgcta aatcactggg
     1021 cgatatcacc cgtctaccca agtcactcaa agttttgctc gaaaacctgc tgcgctggca
     1081 ggatggtaac tcggttaccg aagaggatat ccacgcgctg gcaggatggc tgaaaaatgc
     1141 ccatgctgac cgtgaaattg cctaccgccc ggcaagggtg ctgatgcagg actttaccgg
     1201 cgtacctgcc gttgttgatc tggcggcaat gcgcgaagcg gttaaacgcc tcggcggcga
     1261 tactgcaaag gttaacccgc tctcaccggt cgacctggtc attgaccact cggtgaccgt
     1321 cgatcgtttt ggtgatgatg aggcatttga agaaaacgta cgcctggaaa tggagcgcaa
     1381 ccacgaacgt tatgtgttcc tgaaatgggg aaagcaagcg ttcagtcggt ttagcgtcgt
     1441 gccgccaggc acaggcattt gccatcaggt taacctcgaa tatctcggca aagcagtgtg
     1501 gagtgaattg caggacggtg aatggattgc ttatccggat acactcgttg gtactgactc
     1561 gcacaccact atgatcaacg gccttggcgt gctggggtcg ggcgtgggtg ggatcgaagc
     1621 agaagccgca atgttaggcc agccggtttc catgcttatc ccggacgtag tgggctttaa
     1681 acttaccggt aaattacgtg aaggtattac cgccacagac ctggttctca ctgttaccca
     1741 aatgctgcgc aaacatggcg tggtggggaa attcgtcgaa ttttatggtg atggtctgga
     1801 ttcactaccg ctggcagatc gcgccaccat tgccaatatg tcgccagaat atggtgccac
     1861 ctgtggcttc ttcccaatcg atgctgtaac cctcgattac atgcgtttaa gcgggcgcag
     1921 cgaagatcag gttgagttgg tcgaaaaata tgccaaagcg cagggcatgt ggcgtaaccc
     1981 gggcgatgaa ccgattttta ccagtacgtt agaactggat atgaatgacg ttgaagcgag
     2041 cctggcaggg ccaaaacgcc cacaggatcg cgttgcactg cccgatgtac caaaagcatt
     2101 tgccgccagt aacgaactgg aagtgaatgc cacgcataaa gatcgccagc cggtcgatta
     2161 tgttatgaac ggacatcagt atcagttacc tgatggcgct gtggtcattg ctgcgataac
     2221 ctcgtgcacc aacacctcta acccaagtgt gctgatggcc gcaggcttgc tggcgaaaaa
     2281 agccgtaact ctgggcctca agcggcaacc gtgggtcaaa gcgtcgctgg caccgggttc
     2341 gaaagtcgtt tctgattatc tggcaaaagc gaaactgaca ccgtatctcg acgaactggg
     2401 gtttaacctt gtgggatacg gttgtaccac ctgtattggt aactctgggc cgctgcccga
     2461 tcctatcgaa acggcaatca aaaaaggcga tttaaccgtc ggtgcggtgc tgtccggcaa
     2521 ccgtaacttt gaaggccgta tccatccgct ggttaaaact aactggctgg cctcgccgcc
     2581 gctggtggtt gcctatgcgc tggcgggaaa tatgaatatc aacctggctg ctgagccgat
     2641 tggtcatgat cgtaaaggcg acccggttta tctgaaagat atctggccat cggcacaaga
     2701 aattgcccgt gcggtagaac aagtctccac agaaatgttc cgtaaagagt acgcagaagt
     2761 ttttgaaggc acagcagagt ggaaggaaat taacgtcaca cgatccgata cctacggttg
     2821 gcaggaggat tcaacctata ttcgcttatc gcctttcttt gatgaaatgc aggcaacacc
     2881 agcacctgtg gaagatattc acggtgcgcg gatcctcgca atgctggggg attcagtcac
     2941 cactgaccat atctctccgg cgggcagtat taagcccgac agcccggcgg gtcgatatct
     3001 acaaggtcgg ggtgttgagc gaaaagactt taactcctac ggttcgcggc gtggtaacca
     3061 tgaagtgatg atgcgcggca ccttcgccaa tattcgcatc cgtaatgaaa tggtgcctgg
     3121 cgttgaaggg gggatgacgc ggcatttacc tgacagcgat gttgtctcta tctatgatgc
     3181 cgcgatgcgc tataagcagg agcaaacgcc gctggcggtg attgccggga aagagtatgg
     3241 atcaggctcc agccgtgact ggacggcaaa aggtccgcgt ctgcttggta ttcgtgtggt
     3301 gattgccgaa tcgtttgaac gaattcaccg ttcgaattta attggcatgg gcatcttgcc
     3361 gctggaattt ccgcaaggcg tgacgcgtaa aacgttaggg ctaaccggga aagagaagat
     3421 tgatattggc gacctgcaaa atctacaacc cggcgcgacg gttccggtga cgcttacgcg
     3481 cgcggatggt agccaggaag tcgtaccctg ccgttgtcgt atcgacaccg cgacggagtt
     3541 gacctactac cagaacgacg gcattttgca ttatgtcatt cgtaatatgc tgaagtaaca
     3601 agtatttgct tgccggttat tttgcttccg gcaagcaaat taattccaca atgcgagagg
     3661 gttatttgtt cagcaaatga cccattttct cggctttggt atcgagataa tgttcgttat
     3721 tggggttacg acctacaatc aatggtacgc gttcaacaat attaatccct gcttcggtca
     3781 gaatttcgac ttttttcggg ttattggtta acaagcggac ttcattgacg ccaaggagtt
     3841 tgaacatatc agcgcaaaga gtgaagtcgc gctcatcagc ggcgaagcct aactggtggt
     3901 tagcctctac ggtatcgtaa ccttgatcct gcagtgcgta agcgcggatt ttattcagca
     3961 gaccaatgtt acgaccttcc tgacggtgat acagcaaaat accacggcct tcctcggcaa
     4021 tttgcgtcaa tgccgcttcg agctggaagc cacaatcgca gcgcaagctg aacagggcgt
     4081 caccggtcag acattcggaa tggacgcgcg caagtaccgg ggtatgcccg gaaatatcgc
     4141 catagactag cgcgacatga tcgtgtccgg ttgccagttc ttcaaatccc accatcagga
     4201 aatcgcccca tggggttggc agtttggctt ctgccacacg tttaagctgc atgaaattct
     4261 ccagataatg ctggttctgt attggcttat tttgccataa cgagaagggg ttcacctaat
     4321 cacgacgcgt cgatcgttca cggaatggca caaatctgtc aatttttcct ggaactggcg
     4381 ttttcagtta tgattgtggg acttatcaaa aaggagaggc catgcgttcg attgccagac
     4441 gtaccgcagt gggagctgca ctattgcttg tcatgccagt agccgtatgg atttctggct
     4501 ggcgttggca acctggagaa caaagttggc tactaaaagc ggctttttgg gttactgaaa
     4561 ctgtcaccca gccctggggc gtcattacac atttgatttt attcggctgg tttctctggt
     4621 gtctgcgttt tcgcattaag gctgcctttg tattatttgc cattctggcg gccgcaatcc
     4681 ttgtgggaca aggcgttaaa tcctggatca aagacaaagt ccaggaacca cgaccttttg
     4741 ttatctggct ggaaaaaaca catcatattc cggttgatga gttctacact ttaaagcgag
     4801 cagaacgcgg aaatctagtg aaagaacagt tggctgaaga gaaaaatatc ccacaatatt
     4861 tgcgttcaca ctggcagaaa gagacggggt ttgcctttcc ttccggtcac acgatgtttg
     4921 ctgccagttg ggcactgctg gccgttggtt tgctgtggcc gcgtcggcga acgttaacca
     4981 ttgctatctt gctggtctgg gcaacgggag tcatgggaag ccgcctgctg ctcgggatgc
     5041 attggccacg cgatctggta gtagctacgt tgatttcgtg ggcgctggtg gcggtggcaa
     5101 cgtggcttgc gcaacgaatt tgtgggccat taacaccacc tgcggaagaa aatcgcgaaa
     5161 tagcgcaacg agaacaagaa agttaacgct ggttgatttt ccgaatttag cccttaaatc
     5221 atcaacaatg cgcgtggatg ccatttcgca gacggcgcga aaatggtact ttaaagggct
     5281 attgcggtaa gttgaccata atttattcgc tctaaccaca taacgggaag taatgtgaaa
     5341 tatttactca ttttcttact ggtgttagcg atcttcgtga tttcggtcac gttgggtgcg
     5401 cagaacgatc aacaggtgac gtttaattat ctgttagcgc aaggggagta ccgtatttcc
     5461 acattgctgg cggtattgtt tgctgcgggg tttgctatcg gttggttgat ttgtggcctg
     5521 ttctggctgc gagttcgtgt ttccctggcg cgcgctgaac gtaaaataaa gcgactggaa
     5581 aaccagcttt cacccgcgac tgacgtggct gtagtgccgc actcgtcagc ggcgaaggaa
     5641 taactttcta tgctggagtt gttgtttctg cttttgcctg tagccgctgc ctatggctgg
     5701 tatatgggcc gcagaagtgc gcaacaaaac aagcaagatg aagccaaccg cttgtcgcgt
     5761 gattacgtag cgggggttaa cttcctgctt agtaatcaac aggataaagc ggtagacctg
     5821 tttctcgata tgcttaaaga ggatacaggc accgttgaag cccaccttac gctcggaaac
     5881 ctgttccgtt cgcgtggcga agttgatcgc gctattcgca tccatcagac cctaatggaa
     5941 agcgcctcgc tgacctatga acagcgtctg ttggcgattc aacaactagg gcgtgattac
     6001 atggccgccg ggttatacga ccgcgcggaa gacatgttca atcagctgac cgatgaaact
     6061 gacttccgca ttggcgctct gcaacagttg ctacaaatct accaggctac cagtgagtgg
     6121 cagaaagcaa ttgatgttgc cgaacgcctg gtgaagctgg gtaaagataa acagcgcgtc
     6181 gaaattgccc atttctactg tgagttagcc ctgcagcata tggccagcga cgatctcgat
     6241 cgtgccatga ccttgctaaa aaaaggggcg gcggcagata aaaacagcgc ccgcgtatcc
     6301 atcatgatgg gacgcgtgtt tatggcgaaa ggagaatacg ccaaagccgt cgaaagtttg
     6361 caacgcgtga tatcccagga cagagaactg gtcagcgaaa cgctggaaat gttgcaaacc
     6421 tgctatcagc agttgggtaa aactgccgaa tgggcagaat tcctgcaacg cgcggtggaa
     6481 gagaacaccg gtgccgatgc tgaattgatg cttgctgata tcatcgaagc gcgcgacggt
     6541 agtgaggccg cacaggtcta tattacgcgc cagcttcagc gtcatccgac catgcgtgtg
     6601 ttccataagt taatggatta ccacttaaat gaagcggaag aagggcgtgc caaagagagc
     6661 ctgatggtgc tgcgtgacat ggttggcgaa aaggtacgta gtaagcctcg ttatcgctgc
     6721 cagaaatgtg gttttaccgc atacaccctc tactggcatt gtccgtcttg tcgggcctgg
     6781 tcaaccatta aaccgattcg cggtcttgat ggcctgtaat ttttaaaaaa atccgacttt
     6841 agttacaaca tactagttat tggtgttcca ttgtgctccg gcaacgacgg agcagaaaaa
     6901 gcctgccagg ggaaaaatcg caactgttaa ttttttattt ccacgggtag aatgctcgcc
     6961 gtttacctgt ttcgcgccac ttccggtgcc catcatcaag aaggtctggt catgacgtta
     7021 actgcttcat cttcttcccg cgctgttacg aattctcctg tggttgttgc ccttgattat
     7081 cataatcgtg atgacgcgct gtcctttgtc gacaagatcg acccacgcga ttgtcgtctg
     7141 aaggtcggca aagagatgtt tacattgttt gggccacagt ttgtgcgcga acttcaacag
     7201 cgtggttttg atatctttct tgacctgaaa ttccacgata ttcctaacac tgcagcgcac
     7261 gctgtcgctg ctgcagctga cttaggcgtg tggatggtga atgttcatgc ctctggtggg
     7321 gcgcgtatga tgaccgcagc gcgtgaggca ctggttccgt ttggcaaaga tgcaccgctt
     7381 ttgattgctg taacagtgtt gaccagcatg gaagctagcg acctggccga tcttggcgtg
     7441 acactgtcac ctgcagatta tgcagaacgt ctggcggcac tgacgcaaaa atgtggcctt
     7501 gatggtgtgg tgtgttctgc tcaggaagct gtgcgcttta aacaggtatt cggtcaggag
     7561 ttcaaactgg ttacaccggg cattcgtccg caggggagtg atgctggtga ccagcgccgc
     7621 attatgacgc cagaacaggc gttggcggct ggtgttgatt atatggtgat tggtcgcccg
     7681 gtaacgcaat cggtagatcc agcgcaaaca ctgaaagcga tcaacgcctc tttacagcgg
     7741 agtgcatgat gagtgattcc aacagccgtc tggtctactc aacggaaacc ggacgtattg
     7801 atgagccaaa agcggcccca gtacgcccta aaggcgatgg tgtggtgcgt attcagcgtc
     7861 agaccagtgg acgtaaaggt aagggcgttt gcctgattac cggtatcgat ctcgatgatg
     7921 ccgaactgac aaaacttgca gcggaactga agaaaaaatg cggctgcggc ggagcagtta
     7981 aagatggagt tattgaaatc cagggcgata agcgtgattt attaaaatca ctacttgaag
     8041 cgaaagggat gaaggtaaaa ctcgcaggcg gttaacataa aaagccacgg atatatccgt
     8101 ggctttcgaa tattttactg tgtgtattat tcagttattt cgaactgaac aaaatcaagc
     8161 gtattataat tgcgagtcga taatcgacat ttatttacga ttatttaccg acctggtgac
     8221 caataacacc accgacagct gcaccaccta atgtacccaa cgtactgcca tcggtcagta
     8281 ctgcaccgcc taatgcccct gcacccgcgc cgattgcggt gttgcggtcc cgtttagacc
     8341 agttagaaca ggcactcaga gacattgcca aagtaattgc cagaacagcc gcggtcattt
     8401 ttttgctcgt tacaaacata atactctctc ctgaatttat gattcacgga agtaagctct
     8461 ctataactat aatagctaag aataagcctg gtgaaattat tccgtgaaat ctgctcgcgc
     8521 gcagggttat atcacgcagg tgataatgac ttcctgttat atcgctgata ataattttat
     8581 atcttaagcg tgttaataac aggtaaatag tcttaattat caaccaggaa tcatcttaga
     8641 gcggatgatt tgccaaactg caaatcatcc gtagagaagg gaaatggtta aatgtcaacg
     8701 acgtgaacgg tgagtttact gtgctcaaga tgcatcagat cgctggcgcg tattttcgaa
     8761 tcggtaatca cgcgattgaa tcgctcaacg ggaccgatgg agtatgaatg tacagcacca
     8821 aatttcgagc tgtcagtcag gactatcact tcgcactctt tttccagcac ggcattgacc
     8881 acatcggtac gcatcatatc gcgaccggtg aatccagttt caggttgcca gccatcaata
     8941 ccaataaatg ctttgctgaa atgcacctgc tggatgcact ggcgtgtcaa cgggccaacc
     9001 atactttcgc tttttttctg gtacacgcca ccgagcaaaa tgacttcaca aggcgcgtct
     9061 ttcagcaaat gcgcgatgta gctgctgacc gtgatgatag tgacattttt cttctgctcc
     9121 cccagagtcc gggcgagcag ggcattgctg ctgccatttt cgataaagat ggtttcgccc
     9181 ggttgaacca gtgacgcggc aaactcggcg agttcacgct tcagcgtata gttgctcatc
     9241 atacgggttt cgacgtcatc actatcaagc gaaactgcaa agccatgtgc acggcggagg
     9301 taactcagtt tttcgagggt gttgagatcc tggcgaatgg taacttcaga aactccagtg
     9361 gcttttgcca gatcggttac gctaacctga ccctggtcaa tgaccatctg tagaatagtt
     9421 tgttgtcggg aattcatcgc tgtaattctt ataacgttat aatactaaat aaaaaaatac
     9481 tgacgcgccg ggcatcctca aataggaaaa cctgaaggca cgattgttaa atctcccacg
     9541 gtgccttcgg gatgtctggc gtagattcag caacctggcg caagagttct gctttaagta
     9601 tctcgagatt atggatcgca gagtggtaat cgcctgccac aaggatatct aacacaatat
     9661 caatacgttc ggcaacgcgc tgggcatcaa attcagacat caaggatatc cttatagcaa
     9721 aagtggaaaa tgaataatgc aaaaattgcc gctaaaagag aagtgtttaa cagcaacggc
     9781 taattatcat ccaggaatac gatatataat gacgggatat agcgctaagt atatatattc
     9841 atctacttat gcgcgcttca gatagcgttt ataccagcgt tcgaaggcga cggcgggcat
     9901 cggtttggca aacaaaaatc cttgccgctc attgatcccg ttcttggtta aaaaagcatc
     9961 ttcctttgca ctctctacac cttcggcgat cacctgaaga ttcaatgcct gggccacagc
    10021 gacgatcgcc cggaccagtg actgcgagac aggttgtttg tgaatatctc gaacaaaaac
    10081 ctggtcaagt ttgatggcat cgatcggaaa gcgcgccagt tgcgaaagtg aagagtagcc
    10141 ggtaccaaaa tcatccagat gcacttgcgc acctagttgg ctaaattgtt gaataacaga
    10201 cagtgccagt tcatcattct caatcagaca actctctgtc agttcaacat ctatagggca
    10261 gtattcaaaa ttgagttcct ggagaacctg tttcagggcg gtgaaaatgg tttgatcggc
    10321 gagctgacgt gcagaaatat ttaccgccac tcgcaggttt atgcctttat cccgccactt
    10381 tgccacctgg cgtacgacat cgagaatcac ccaacggcct aaaggcacaa ttagccctga
    10441 ctcttcggcg taggaaatga agtccaacgg tggaatcaac ccacgttcag gtgactgcca
    10501 acgtactagt gcttccagac tgcgcacttc gccacgctag gtgattttcg gttgatagtg
    10561 aataaccaac tgatcgtttt ccagtgcttt acgcaagttg gtatccagcc agagatattc
    10621 aaatacccgt tgattcattt ctggggtaaa aacgcaaaat tgtcctcgtc cgccttcctt
    10681 cgctgtgtac attgctgtgt cggcgtgacg aataatagcc gtgctgtctg aaccatgttc
    10741 gggagagagt gcaataccta ctgaacagct ggtataaact tcaattaaac caatgcgaaa
    10801 ggggagccgt aagcgggtca aaattcgtga tgccattgct tccagcgcgc tttgtgaggt
    10861 gttggatgcc agtaccagaa actcatcccc acctggacgc gccaacacct ggtcatgttc
    10921 gagacagctt aaaatagcca atgacacgtc gcgtaataac tggtcaccaa acaaatgccc
    10981 ataggcgtcg ttgacctttt tgaaattatc caaatcaaga tacacaaccc caactttata
    11041 gttatctgca tgattaatag cgtgatcgat taaatcctgc attgcgttac gattcggcag
    11101 tccggtgata ctgtcggtat ttgccagaat acgcagtcgc tcctgagcgc ggcgctcttc
    11161 ggtaatgtcg gtgccggaac agattaaaaa aatctcgttt ttgccactgc cgctgtggac
    11221 aaatttattg cgaaacagaa acagccgctg gcctttacat gttggtatcc acagttcgac
    11281 ttcatatgca ttgccgcttc gaaaaaatac acggttattg cgcctggatg tcgcagcttc
    11341 acgacggctc ataaacagtt taaacacgct ttggccaatg acgtcgtgtt ctttcaaccc
    11401 agtgtaatct tcacataacc gattgaagcg ttggatattc ccccggctgt cgagaatcac
    11461 tattgcggaa tgtgcttcag aaactacctg ctcagcaaag gaaagcccgt gtgataaatc
    11521 acgagcaact gccggtgtgt catgccatgc agacgcgttg ccagcccatt cccgtttatt
    11581 gatttttcgt cctaccagat gcacagaaag atcgctctca tcgacggtca gactcatcat
    11641 caggctggag gtgatgaccg tcatttccct gatgcgagca gcctgttcgg cagataactg
    11701 caaagtacga tcaggttctg tggtttcggt ggtagaaaag cgcaaaacat cgctgctttc
    11761 cgtcaaccgc cagtatggat tgtgcgatcc gagaatgtgg actccctaac ggttttcatg
    11821 atgcatatcc tccggttaac agcggagtcc gcggttaaat aaaaggaaca acat
//
LOCUS       DAJTMK010000127        11835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTMK010000127 DAJTMK010000000
VERSION     DAJTMK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.129"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            131..196
                     /gene="yobI"
                     /locus_tag="OO135_002972"
     CDS             131..196
                     /gene="yobI"
                     /locus_tag="OO135_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5398740.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            335..946
                     /gene="ruvA"
                     /locus_tag="OO135_002973"
     CDS             335..946
                     /gene="ruvA"
                     /locus_tag="OO135_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5398741.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            955..1965
                     /gene="ruvB"
                     /locus_tag="OO135_002974"
     CDS             955..1965
                     /gene="ruvB"
                     /locus_tag="OO135_002974"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5398742.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2015..2800)
                     /gene="znuB"
                     /locus_tag="OO135_002975"
     CDS             complement(2015..2800)
                     /gene="znuB"
                     /locus_tag="OO135_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5398743.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLTGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2797..3552)
                     /gene="znuC"
                     /locus_tag="OO135_002976"
     CDS             complement(2797..3552)
                     /gene="znuC"
                     /locus_tag="OO135_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5398744.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPSTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3631..4563
                     /gene="znuA"
                     /locus_tag="OO135_002977"
     CDS             3631..4563
                     /gene="znuA"
                     /locus_tag="OO135_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5398745.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPGMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4579..5901
                     /gene="mepM"
                     /locus_tag="OO135_002978"
     CDS             4579..5901
                     /gene="mepM"
                     /locus_tag="OO135_002978"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5398746.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6021..6992
                     /gene="lpxM"
                     /locus_tag="OO135_002979"
                     /gene_synonym="msbB"
     CDS             6021..6992
                     /gene="lpxM"
                     /locus_tag="OO135_002979"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5398747.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRCNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(7081..10157)
                     /locus_tag="OO135_002980"
                     /pseudo
     CDS             complement(7081..10157)
                     /locus_tag="OO135_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(10195..>11835)
                     /locus_tag="OO135_002981"
     CDS             complement(10195..>11835)
                     /locus_tag="OO135_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR5398748.1"
                     /translation="SETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYI
                     GIYDSALKFNCKAENGEFWAQATALRFVLGCYESTRNEGLLTSLTQAAQCIMAGYPKE
                     TSHPFVVEHGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQ
                     YKNVINPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIG
                     DEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHP
                     IHHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVEN
                     MFQENNNGFTALFYGPCEFYGTYNNVAINITQLTEYPHDLSVKCLIEPESSVRFALSF
                     RYPDWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDIKFIADIQFNTDLCGDTYI
                     SRGPVLYAIELESDILIKKTLLNWKYFDSGYIPCSRADESIQFSYTDMESFSYSTSPE
                     GKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
BASE COUNT         3321 a         2627 c         2701 g         3186 t
ORIGIN      
        1 acctatacat gtaaagacga tatctccaat ttcatattca gcagggtaat taatattcac
       61 gttgtcattc cattgaaata gatacgaata tacgtaatag cataaaaaaa ttattttttc
      121 aggaggcgca atgtatattt ttattacgca tttcttcact gaatacgtaa tatttaaata
      181 tttgcttcca atataacctg tagaataaat catactgcgc catttttcag ttcatcgaga
      241 cacctcgcaa gttttcttca tccttcgctg gatatctatc cagcattttt ttatcataca
      301 gcattatctt tgattcatta cgcaggagcg tcatgtgata ggcagactca gaggcatcat
      361 cattgaaaaa caacccccgc tggtgttaat tgaagtgggc ggcgtaggct atgaagtgca
      421 tatgccgatg acctgttttt atgaactccc tgaagcgggt caggaagcga tcgttttcac
      481 ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt tttaacaata aacaagagcg
      541 cacattgttc aaagagttga tcaaaaccaa cggcgtcggc ccgaagttgg cgctggcgat
      601 cctctccgga atgtcagcgc agcagttcgt taatgccgtt gaacgtgaag aagtgggggc
      661 actggtgaaa ctgccgggta ttggcaaaaa aaccgccgaa cgcttgattg ttgaaatgaa
      721 agaccgattt aaaggtttgc atggcgatct gtttaccccg gccgccgacc tggtactcac
      781 atcacctgcc agcccggcga ccgacgatgc tgaacaggaa gcggttgccg cgctggtggc
      841 gctgggctat aaaccacaag aagcaagccg catggtgagc aaaatcgctc gccctgacgc
      901 cagcagtgaa acattaattc gcgaagccct acgcgccgcg ttatgaggta aaggatgatt
      961 gaagcagacc gtctgatttc tgccggtacc actttgccgg aagatgtagc agatcgcgcc
     1021 attcgcccta aattactgga agagtatgtt ggtcagccgc aggttcgttc acagatggag
     1081 attttcatca aagcagcgaa actgcgcggc gatgccctcg atcatttgtt gatttttggt
     1141 cctccggggt tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat
     1201 ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg atctggcagc gatgctcact
     1261 aaccttgaac cgcatgacgt gctgtttatt gatgagatcc accgtttatc gccagttgtt
     1321 gaagaagtgc tgtacccggc aatggaagac taccaactgg atattatgat tggtgaaggt
     1381 ccggcggcac gctccattaa aattgatttg ccgccgttta ccctgattgg cgcaaccacg
     1441 cgcgcaggtt cgctgacatc accgttgcgc gaccgttttg gtattgtgca acgtctggag
     1501 ttttatcagg tgccggatct gcaatatatc gttagtcgca gtgcacgctt tatggggctt
     1561 gagatgagtg atgacggcgc gctggaagtt gctcgtcgcg ctcgcggtac gccacgtatt
     1621 gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc
     1681 tcggcagata tcgctgctca ggcgctggac atgttgaatg tcgatgctga aggtttcgat
     1741 tatatggacc gcaaattgtt gctggcggta atcgataagt tctttggtgg gccggtaggt
     1801 ctggataacc tggcggcggc tattggcgaa gaacgtgaaa ccattgagga tgtgctggaa
     1861 ccttatttga ttcagcaagg ctttttgcag cgtacaccgc gtgggcgtat ggcgacgacg
     1921 cgggcgtgga atcactttgg cataacgccg ccagaaatgc cgtaagtcgg attggtcgct
     1981 cgcgccgcat ccgacaaatg tgttcagcga tagattagct ggcctgcttt ttcatcatac
     2041 tgataataaa taacagtgct gcacatagca ccaccgacgg acccgccggc gtatcgtaaa
     2101 atgcggaaaa ggttaaaccg cccgttacgg caaccatccc caccaacaca gcgacacctg
     2161 ccatctgttc cggcgtgcgg gcaaagcgac gtgcagtagc agcaggaata atcagcagtg
     2221 aagtaataat caacgcgcca acaaatttca tcgctacacc aatcgtcaat gccgtcacca
     2281 gcatcaacaa caatttcacg cgctgtaatt tcacaccatc aacaaacgcc agatccgggc
     2341 taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa aatagccacc acgatgacca
     2401 cgccaatcgc aatagcgatg agatcttctg gcgtcactgc cagcaaatca ccgaacaggt
     2461 aagccatcaa atcaacacga atgttagaca tcagactaac gaccaccagg cccagcgaca
     2521 gggcactgtg cgccataatc cctaataacg tgtcaatcgc cagctgtgga cgcttctcca
     2581 gccataccag accgcccgtc aggagcagcg taacggcaat taccgcatag aatggattca
     2641 cgtccagcaa caaaccaaac gcgacgccaa gtaatgaggc atgagccagc gtatcaccga
     2701 aataagacat acgacgccag actacaaacg aacccagcgg acccgcggca caggcgagca
     2761 tgatcccggc taaccaaccg ggaaataata attcaatcat gagcgatcat ttccccgacg
     2821 caaaacaatt cgtccctgta aatcgtgacg atgattatga tgatggcgat agatacccag
     2881 ttgttcagca ccacgaggac caaacataga aataaactcc ggatgtaggg aaacaacttc
     2941 cggtgtgccg gaacaacaaa tgtggtgatt cagacacagc acttcatcgg tttttgccat
     3001 taccagatgc agatcgtgag agaccattaa aacgccacaa tccagttcgc gacgcaactg
     3061 gtcaataagg tcatataacg ccacctggcc atttacatcc acgccttgag tgggttcatc
     3121 cagcactaat aattgcggtc gatttaacaa tgctcgcgct aacagtacac gctgtgtttc
     3181 gccaccagag agcttttgca tcggtgcgtt aatcagatgc ccggcctgga cacgtttcag
     3241 tgcaggcaaa atatcttctt tatgtgtgct agggcgtaag cgtaaaaaac ggtttacggt
     3301 cagtggcaac gtggtgtcga gatacagctt ctgcggtaca tagccgatgc gtagttttcc
     3361 gttgcgcttg ataacccctt catcaggtgt taccagcccg agcactaccc gtaccagtgt
     3421 cgacttacct gcgccatttg gcccaagtaa agtcaaaatt tttccaggtt taagttccag
     3481 cgacacatca gagaggacgc ggcgttggcc aaaagaaacc gagacatttt ccagggaaac
     3541 cagacttgtc atgttaattt tagtcttgca gtagtcatga aatgttataa tatcacactt
     3601 ctcatattca ttacgatgat tggtcgcatt atgttacata aaaaaacgct tcttttcgca
     3661 gcattatccg ccgctctctg gggaggtgca acacaggccg cagatgccgc cgttgtcgct
     3721 tcgcttaaac ccgttgggtt catcgcttct gccattgctg atggtgtaac agaaacggag
     3781 gttttactgc ctgacggcgc ttcagaacat gattattcac tgcgtccatc ggatgtaaaa
     3841 cgcttacaga acgcggactt agtcgtttgg gttggcccgg ggatggaagc gtttatgcaa
     3901 aaaccggtaa gcaaattact aggagcgaag caggtaacga ttgcgcagct tgaagatgtg
     3961 aaactgctac tgatgaaaag tattcacggc gatgatgatg atcacgacca cgcggaaaaa
     4021 agtgaagaag atcaccatca cggcgatttc aacatgcatc tttggctttc cccagagata
     4081 gcgcgggcta cagcggttgc aatccatgga aaattagtgg aacttatgcc gcaaagtcga
     4141 gccaaacttg acgccaacct gaaggatttt gaggcacaat tagcctcaac cgaaacgcag
     4201 gttggtaacg agctcgcgcc gctcaagggg aaaggttatt tcgtttttca cgatgcttac
     4261 ggctatttcg aaaaacagtt cggactgaca ccgcttggtc attttaccgt taaccctgag
     4321 attcaacctg gcgcgcagcg tttacatgaa ataagaacac agttggttga gcaaaaagca
     4381 acctgcgttt ttgctgagcc acagttcagg ccagcggtcg ttgagagcgt cgcacgaggg
     4441 acatccgttc gtatgggaac gttggatccc ttggggacga atatcaaact gggtaaaaca
     4501 agctattcag aattcctgag tcaattagcc aaccagtatg cgagctgcct gaaaggagat
     4561 taatgaggaa gtgattacgt gcaacagata gcccgctctg tcgccctggc gtttaataat
     4621 ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg ttcttactct ggccgtcgct
     4681 gtctggcggc cctatgttta tcaccgcgat gccacgccaa ttgtcaaaac cattgagctg
     4741 gaacagaacg aaattcgttc gctcttacct gaagccagtg agccgattga tcaagctgca
     4801 caagaagatg aagccattcc ccaggacgaa ctggatgaca aaatcgccgg tgaagcgggc
     4861 gtgcatgaat atgttgtttc cactggcgat acgctaagca gcattctcaa tcagtatggt
     4921 attgatatgg gtgatatcac ccaactggct gcggccgaca aagaattgcg taacctgaaa
     4981 atcggtcaac aactctcctg gacattaacc gcggacggcg aactgcagcg cctcacctgg
     5041 gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg ccgctaacgg ttttaaaatg
     5101 accagcgaaa tgcagcaagg agagtgggtt aacaatctgc tgaaaggtac cgtcggaggg
     5161 agctttgttg ccagcgccag aaacgccggt ttaaccagcg ccgaagtgag cgcagtgatt
     5221 aaagccatgc agtggcaaat ggatttccgc aaactgaaaa aaggcgatga atttgcggtg
     5281 ttaatgtctc gagagatgct tgatggtaaa cgtgagcaaa gccagctgct gggcgtacgt
     5341 ttgcgttcag aaggtaaaga ttattacgca atccgcgctg aggatggcaa attctacgac
     5401 cgtaacggta ctggtctggc gaaaggattc ttgcgattcc cgacggcgaa acagttccgt
     5461 atctcgtcta actttaaccc gcgtcgtact aatccggtga ccggtcgcgt tgcaccacac
     5521 agaggtgttg atttcgccat gccgcagggt acgccagtgc tttcagtggg tgacggtgaa
     5581 gtggtggttg ccaaacgcag tggcgcagca ggttattatg tggctattcg tcatggtcgc
     5641 agctacacca cgcgttatat gcacttgcgc aagattctgg tgaaaccggg acagaaggtg
     5701 aaacgtggcg accgtatcgc gctttccggt aataccggac gttcaaccgg gccgcatctg
     5761 cactatgaag tatggataaa ccagcaggcc gtaaacccgc tgacggcaaa actgccgcgt
     5821 accgaagggc tgaccggctc cgatcgtcgc gaattcctgg cgcaggccaa agagattgtg
     5881 ccgcagctac ggtttgatta attaacatcc attcgcagcc ggtacgcagt cagtaccggc
     5941 tttttttatt tggtgcgggg caagttgcgc cgctacacta tcaccagatt gatttttgcc
     6001 ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa tagcgaatac attcctgagt
     6061 ttgataaatc ctttcgccac ccgcgctact ggggagcatg gctgggcgta gcagcgatgg
     6121 tgggtatcgc tttaacgccg ccaaagttcc gtgatcccat tctggcacgg ctgggacgtt
     6181 ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt aatcaatctg tcgctctgct
     6241 ttccagaacg tagtgaagct gaacgcgaag cgattgttga tgagatgttt gccaccgcgc
     6301 cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg accggagaaa attcagccgc
     6361 gcgttgactg gcaagggctg cagatcatcg aagagatgag gtgtaataac gagaaagtta
     6421 tctttctggt gccgcacggt tgggccgtcg atattcctgc catgctgatg gcctcgcaag
     6481 ggcagaaaat ggcagcgatg ttccataatc agggcaaccc ggtttttgat tatgtctgga
     6541 acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag aaatgacggt attaaaccat
     6601 tcatccagtc ggtacgtcag gggtactggg gatattattt acccgatcag gatcatggcc
     6661 cagagcacag cgaatttgtg gatttctttg ccacctataa agcgacgttg cccgcgattg
     6721 gtcgtttgat gaaagtgtgc cgtgcgcgcg ttgtaccgct gtttccgatt tatgatggca
     6781 agacgcatcg tctgacgatt caggtgcgcc caccgatgga tgatctgtta gaggcggatg
     6841 atcatacgat tgcgcggcgg atgaatgaag aagtcgagat ttttgttggt ccgcgaccag
     6901 aacaatacac ctggatacta aaattgctga aaactcgcaa accgggcgaa atccaaccgt
     6961 ataagcgtaa agatctttat cctatcaaat aaaaaaaagc ctctcaaatt attgagaggc
     7021 tgtactcgat aaaaaatctc aatgagtgtt cccactattt tgagttttta ggttagtacg
     7081 ctattgagtt aatcgtagcg tgataatttc aaaaggtttg aactcaagat tgcaatattg
     7141 attgcaataa aaactgtcat ctaacttatt ttcttgaaga tcgacatatt gaacgtagtg
     7201 ataattacat acccacgaaa gcatggctga aatttctaat ccatgactct catataatct
     7261 tacgattata ccgtcgccgt cttcagctaa ttttattgtt tcgatgataa cgctgttagt
     7321 atcgatagca aaaaatgata gtgaaggggg taattcaccc gggtgcggtt tgacagggac
     7381 gacctgtaag ggatgattca ttatataggc ttgattaata acaccaccca cacggtaatc
     7441 tccatgatga ggaaataagg agaatatgaa tttttgttta ccaatatctg caaaattatt
     7501 aatcaggaaa ttatcataat tgtctgtctt aacaacttcc ccaggtttat gctgcgaacg
     7561 cagcaaagtt aattcaagcg tctggtcttt gacacgatag ccatatttgt tttttgctat
     7621 taaagctatg ccagtttctc gatcagaaag gtcgacccag tgatgtgctg gtatttcatc
     7681 tttggcaaaa gcgaacgaat catcgctatg cgttggtctg tcaattacac cgaactgaat
     7741 ttgacattgt gcaagagatg tttgtactga caccggggat ttgactttga gagaaacatt
     7801 aggttcgtgc caatcaataa aagtttggac atcaacgcgt ggactgtcct gtgtcagggt
     7861 aaatgcgaag cgaagataag aggaattgta gtgataaatc agcgtcatcg tagttttagg
     7921 cccgctgatt acagttgtca ttttttcagc gagtaaaaca tatgattccc catcacgata
     7981 atttgaagca aaatcccagg ccgcgaaaaa acctgcatct tcgtggtatg cacggatctc
     8041 actgtgcata ttttcagtaa tgtattcttt accatatctt ttatcatata gcgaaattaa
     8101 atcaccgttt tctgaaaaaa gaagtttaat gtaattattt tcaatacttc gagattctgc
     8161 cttcagacca tttacgatct tattctttgg atcaatcaca gcataaccat aagagtttac
     8221 tcgtgctttg agccagttgt tgtttatatt gatccattcg ttacgagcaa aacaggtggt
     8281 attaaataaa atgtgcggat cttcataaga agaagtatca attttactta ataaagtaga
     8341 ctgcgtatca gaaataattt tctcggtttt tgcttctagt ttaatatatt ccttttcagt
     8401 ttcaagataa actcttgaaa tacatgatcc gggaaggatg tcatggaatt gcaaggtcag
     8461 catctctttc catatttcat caaactcact gcgaagaatt gatgtcatat tattagtaat
     8521 agttgtaaaa aattcagcat catgtaattt attttccata tttcgattat gagctttagt
     8581 tgctgactct gatgtaaagc acccttgatg agcctcgaaa taaagttcac ctgaaataat
     8641 gggcaagctt tcgcgaaagg cgtcggcgta agtgaagaac ttatcaacac ggctgaaatc
     8701 aacatgaggt aagccatcaa tattgcgtat acgtgttaat cgttctatat gttcctcacc
     8761 ggggccagcg ccgccatcac cgacaccata caccataagc gcatagggac agatctcttt
     8821 ttcattatag ttttgttcaa tatattctaa agagcgagca cgtgctcggc tatcatacgt
     8881 gtcttcagga aagttatgca tcagaatttc tgagccatcc aggccctgcc agcggaaaag
     8941 gtaattatta aatttattaa tcttgttttg tgagagcttt tgcgatgcaa tatagttaat
     9001 gcctgatttt tttgagaatt tgtggaagcg ttgcggggaa tccaaagaca tccggttgcc
     9061 agacataatt tatctttttt gaaaaattct gcagtgtgaa tcgtgtccca tgcatgattt
     9121 gccttatcag cgattcacca cttaccagat ttaaatcgca ttcgacccag aagcaacctt
     9181 gtaactcaaa gcggcctgcg gcgatttgtt tttttaactt ttcgaagaaa tagggataat
     9241 ctttttttat ccagtgaaaa agttggtact gactagcgcc aaaaatgtaa tcaggatatt
     9301 tttcaatgtt tgcaagggcg gtggcaaatg ttcttattgc ttttctgcgt ccttctctta
     9361 aaggctacat ccacgctata tctaaatgag catgaccagt agccataatc ctgaactctg
     9421 aaggattatt gtttttattg attatggatt ggattgcaat ttgacattta attatttctt
     9481 ccattgctaa aggtaattaa cgtattatca atatattcga gagtacttat gatttcgtca
     9541 gtacgctcac tttttttggc aataaattgt tctggggtga tacctttagg atgtatggat
     9601 tcaaacttct gaccatcatt aatccagtcg aaaagcacct caatatcata ataaagggat
     9661 ttcagtagtt gattacatgt cgcgatatga acatcagtaa taatccctcc attagtaaca
     9721 tagccaaata aatcgttgca ggcagcatca atccaaaact caacctcgtc attttcacaa
     9781 acgagagaaa gatcaaccac ccatttaccc ggtttatctg gcggtacgcc ataagatgat
     9841 gttttattcg tgatgccgca gatgggtgaa ccattcttat ccacaacaag accttcgcca
     9901 ccgatatcaa cgagagcgac gaatttgtgt ctaaatttat cggctggacg tttaccataa
     9961 actcgcatcc aggcacagtc aaacagttga ccccagtctg agccaacggt aagtttctgg
    10021 tatgcgccgc tacggcgata aacaaaagac tgcgattctt ttgttatcca tgcttcaatc
    10081 gacatttcag agacaacagt ataaactctc tgacctattt tattcgacag tttaataata
    10141 ttatcattaa ggtccataga aatcccttta attaaatata atactcgaaa aatgttaact
    10201 ctctatattg gagaaagtta ctttgcgaag aatagtttga ccaaatggta taagttttct
    10261 gactatttta gtcttgttta aataaaaaca cccttcaatg tattgcttgc cctctggtga
    10321 ggttgaatat gaaaaagatt ccatatcggt ataagagaac tggatagatt catcagctct
    10381 actacagggt atgtatcctg agtcaaaata tttccagttc agcagagttt ttttgattaa
    10441 aatatcgctc tcaagctcaa ttgcatataa aacgggacct ctggaaatat aagtgtcacc
    10501 acataagtca gtattaaact gtatatcagc tataaattta atgtcaatca catcatgatg
    10561 ttgccatgtt ctatctaaaa taataagtga gttttgagta tcatttttag taaaagtaac
    10621 accattaata atcatcattt ttgcccagtc ggggtaacgg aaactgagag caaaacgaac
    10681 agatgactca ggctcaatga gacattttac gcttaaatca tgagggtatt cagttaattg
    10741 tgtaatatta attgcaacat tattatatgt gccataaaac tcacacggac cataaaataa
    10801 tgcagtaaaa ccattgttat tttcttgaaa catattttcg acaaaagccg gtatggttct
    10861 tgctgtattc ggtacgcagc agacagcagc ctcctcatga gtaggggagt atttatatct
    10921 ggtattaaac tgattactgt ccggatgttt tctttcattt gcttcataac aattatcagt
    10981 cttacagtac attatcgcgt gatgtatggg atgctgcatg ccaaaactgg cattatagaa
    11041 tagccactca gcatcatcgg ccagaccaat attagtggtc agttttatta acataacata
    11101 agtgctaaat agttcatgaa cagaacaata ttcatagcct gtataagtgg cattagctgt
    11161 gcgtcctgta atccactcat caccaatggg agcgccagat ggtgtaagat aatatttcaa
    11221 ctgggataaa aaaatatcta atgcagtttg atactgcggc cgttggctcg ctgcgatggc
    11281 taatgcccta atatgctcat atgtatgtac gccatgacct ttaagaaggt aaccagggtt
    11341 tatgacattt ttatattgca gatcttgctc cgacacgcat cctgcggaat aatcttcata
    11401 cagccacaag caatatgaga gatattcaat gttattggtt atttcataaa ggcggtagaa
    11461 aacatctgtt attgttaatc cgtggctatg tccagcaaaa ccatgttcca caacgaaagg
    11521 atgtgacgtt tctttagggt agccagccat aatacattgt gcggcttggg taagagaagt
    11581 taataaaccc tcattacggg tggactcata acatccaagc acaaaacgca aggcagtagc
    11641 ttgtgcccag aactctccgt tttctgcttt acagttaaat tttaatgctg aatcataaat
    11701 tccaatgtag ccatcatcat cttgcgtatc gataagttcc aaacataaat cgctcgcttt
    11761 ttcctgccat tctttattat ctaacaagta ggcactgcgg caatatccat ccttccaatt
    11821 cgattgagtt tcact
//
LOCUS       DAJTMK010000128        11509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTMK010000128 DAJTMK010000000
VERSION     DAJTMK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11509
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.130"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..307
                     /locus_tag="OO135_002982"
     CDS             <1..307
                     /locus_tag="OO135_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5398749.1"
                     /translation="VNAIERDKALAWVEKNIKVPLTEPQKSGIASFCPYNIGPGKCFS
                     STFYRKLNAGDRKGACAEIRRWIYDGGRDCRNRSNNCYGQVSRRDQESALACWGIDR"
     gene            528..641
                     /locus_tag="OO135_002983"
     CDS             528..641
                     /locus_tag="OO135_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398750.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            643..1110
                     /locus_tag="OO135_002984"
     CDS             643..1110
                     /locus_tag="OO135_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5398751.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRVHAIIYKEQRDKKASEL
                     ELANATITDMQIRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQDYIRTQCLN"
     gene            1449..1775
                     /locus_tag="OO135_002985"
     CDS             1449..1775
                     /locus_tag="OO135_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCR5398752.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVRFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(1907..2107)
                     /locus_tag="OO135_002986"
     CDS             complement(1907..2107)
                     /locus_tag="OO135_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5398753.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            2149..2504
                     /locus_tag="OO135_002987"
                     /pseudo
     CDS             2149..2504
                     /locus_tag="OO135_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829198.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            2795..3355
                     /locus_tag="OO135_002988"
     CDS             2795..3355
                     /locus_tag="OO135_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5398754.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPPAVMS
                     HGIEYMEDGLPDPVKAMGQILVENLSIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPRLVVNNDNEGNG"
     gene            3352..5013
                     /locus_tag="OO135_002989"
     CDS             3352..5013
                     /locus_tag="OO135_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5398755.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKINDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VIPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCINLDDILRRVDVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFHDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASRRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            5077..7013
                     /locus_tag="OO135_002990"
                     /pseudo
     CDS             5077..7013
                     /locus_tag="OO135_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            7058..7279
                     /locus_tag="OO135_002991"
     CDS             7058..7279
                     /locus_tag="OO135_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398756.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYSEPEKNRNQDVT"
     gene            7225..9647
                     /locus_tag="OO135_002992"
                     /pseudo
     CDS             7225..9647
                     /locus_tag="OO135_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            9644..9970
                     /locus_tag="OO135_002993"
     CDS             9644..9970
                     /locus_tag="OO135_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5398757.1"
                     /translation="MTALLTLEEVKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            9980..10330
                     /locus_tag="OO135_002994"
     CDS             9980..10330
                     /locus_tag="OO135_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5398758.1"
                     /translation="MAISVGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQFGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            10327..10773
                     /locus_tag="OO135_002995"
     CDS             10327..10773
                     /locus_tag="OO135_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR5398759.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVLRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            10770..11114
                     /locus_tag="OO135_002996"
     CDS             10770..11114
                     /locus_tag="OO135_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR5398760.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVAPLLDGRPLVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            11179..>11509
                     /locus_tag="OO135_002997"
     CDS             11179..>11509
                     /locus_tag="OO135_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398761.1"
                     /translation="MSALYERSQLTQVMISSALATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFK"
BASE COUNT         2593 a         2791 c         3624 g         2501 t
ORIGIN      
        1 ggttaacgcc attgaacgtg ataaggcgct ggcatgggtg gagaaaaaca tcaaagtgcc
       61 gctgaccgaa ccccagaaat cggggatcgc gtcattctgt ccgtacaaca ttggccccgg
      121 taagtgtttc agctcaacgt tttaccggaa actgaacgcc ggagaccgga aaggtgcatg
      181 tgctgaaatt cgtcgctgga tatatgacgg cggcagggac tgtcggaacc gctcaaataa
      241 ctgttacggg caggtatcgc ggcgtgacca ggagagcgcg ctggcgtgct ggggtatcga
      301 caggtagcag aatattttgc tgaaaaatga cgcttgctca cgcgggcgga taacacgaag
      361 tcctgtgaac tggcaaaacc taagtgaata aaagtaaaaa ccccgtttgt tggcagcaag
      421 cggggttttg tgttttctga ccttgagtaa ggcaagggag aaattatggt gagggaggtg
      481 ctttccctgt gaggaagtat aaaagattct ttctgaggtt gtccattatg aaaggcattg
      541 aagtggagac gccagccagt ctggatttaa caagagcggc agcttttgcc attcgtattg
      601 tggcaattgc tgttctggtc tgggcaatcc gttggtggtg atatgaaccg tgttctgtgc
      661 gtggtcatca ttgccctgct ggtggcctgt ggtgcgctta gtctggggct gaatcattac
      721 cgtgttcacg ccatcatcta caaagagcag cgcgataaaa aagccagtga gctggagctg
      781 gcgaacgcga caattactga tatgcagata cgccagcgtg atgtcgctgc acttgatgcc
      841 agatactcga gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt
      901 gccgctggtc gtaagcgcct gcggatcaac gccacctgct ccggtaccgt gcgtgaagcc
      961 accggcacct ccggcgtgga taatgcaacc ggcccccgac tggcagacac cgctgaacgg
     1021 gattatttca tcctcagaga acggttgatg acaatgcaga agcagctgga aggggcacag
     1081 gactatatcc gcactcagtg cctgaactaa gttttgctga tgcgccgtat cgtcgctgta
     1141 ttccctcatt aacagagacc gcagcccgac agggagactc ctctgcgcga gtgtgcgggg
     1201 ataatcaaaa acgatacaca ccggggttta ccgcgttaac ggagcgcggc gttgtcccct
     1261 catagtcgcc agtccggtgc gatggtggaa gaaacaggac gatgtgttac ctcgcaagcc
     1321 ctgttatgtc atgtgtctga tttgtgattt aagtcggata attgtcgttg ccattaagca
     1381 gaggattgat gaccgacagg gcggcattgt tagaataaga cttattttta tctgtgcggg
     1441 gaatgaaaat gaaaagaaat cttccgttaa ttattttgtt gtcttctctg gttatgggct
     1501 gtacgcaaca taaaacagat atgccccgac agttggttaa ggcattacca caatatccgg
     1561 cctatgcagc ggcaaattat ataaagggac gggttgatgt gaggtttgat attggtgctg
     1621 atggtactgt cacccgaatt gagtttattc gttcagagcc gcaccatctg tttgatgagc
     1681 aggttgtaaa ggcgatggca aaatggcgat ttgagaagga caggccgtgt aaaggcgtga
     1741 agaaaacgtt tatctttagt ccttccgcac cctgattatt tcatcagaaa ttaattatca
     1801 ctctgttgtt attctgtaca tcccggcagg gtaagtcttg ttccgtcgga tatgaagatg
     1861 aaatattgtt ggaggacagt gggtacctgc tcctgtaacc gaacgttcat ttctcgttat
     1921 ttgtcatgct ggccgggcgc agatgcgttg catctgttgc cagccttctc ctgcaggctt
     1981 caataaccca cgctgaaaag ttaccggacc ctttatgctc aagggcgatg ttgatctgtt
     2041 caatcatgtg attggggaaa cggatattgc gggttgtggt tctgcgggta cggtttttcg
     2101 atgacatatt tatttccttt actgattgcc atatgacggg gattttacat ggctgagctt
     2161 cgtacactcc agagcagaat caaaacactg aatacccgac gggtgaatat tctgaagggt
     2221 gaacagcgtc gtgtcagtgg cagtgcacgt gtttccctca agcgtcatat ctggctcagg
     2281 gacgccgggc agtgccgtct ctgtggtcgt gtggttgacc tctgtgacag tgaactcgat
     2341 caccgaattg cacttcagtt cggtggtggt aatgaggaga cgaatctctg gacgctctgt
     2401 accgaatgcc atcgacaaaa gtctgctcgt gaagcggcga gtgatatgcc ggacccgacg
     2461 ctgccggagg tagtggcaga gaggacgaca tcatcggact gtaacccgac cccggggggt
     2521 atcatccggc gtaaaaaacg atcgctttgg acaccgcgcc ccctctcacg cagagaaaaa
     2581 attcccgttt caggccagtt aacatgttaa ctggctgtcc gggcattttt gcggttttta
     2641 tctttattat tcagtttgtt gtgcgaaaaa aaatgttaac tggctttttc agaaaatgtt
     2701 aaccaggcag cagttaacat ttgcggcatg agacgccggg aaaaatgggc tgaaccatac
     2761 ccggctgagt gcgttctgga cccgggagga ggctgtgctg acaacgcaaa aacgaaaatt
     2821 tgcgctggcg ctcatgtccg ggaaaaacaa aacagcgtca gccattgccg ccggttattc
     2881 ggcgaagacc gccagggtta aaggctcgca gctggcaaaa gatccggagg tgctcgcgtt
     2941 tatagcccgt aaacagtgcg aaacggtgga ggtggatgag gttcctgttt accggcagaa
     3001 aaaatcagag caggaggata aaccccgtcg ccgtgaggcg gctgcaatac cacagccgga
     3061 cgaaaacaat ccggagatgc caccgcccgc ggtgatgtct catggtattg aatatatgga
     3121 ggatggtctt cccgatccgg tgaaagctat ggggcagatc ctggtggaaa acctcagcat
     3181 tgatccgaaa ctggcactgg atgcggcctg gcgactggcg cagttcacac accataaaaa
     3241 aggcgatgcc ggtaaaaaat cggcaaaagg tgatgccgcg aaaaaagcgg ctaaccgttt
     3301 tgcggtacca ccaccacgac tggtggtgaa taacgataat gagggcaacg gatgatacct
     3361 gtatggagca cggcctgccc ggactgggca gagcgcctga aaaaggggct gtcgattatt
     3421 ccggctccga tttatccgga ccaggccgca catgccctgg cgatttttaa acaactgcgg
     3481 attgtggatg caccgggtag cccgacgttc ggggagtcct gtgcacagtg ggtgtttgac
     3541 ctggtggcgg ctctgtttgg ctcctacgat gcgcagaccg gtgtccgcca tatcaaggaa
     3601 gtgttcattc tgatacccaa aaaaaacagc aagtccacac tggccgccgg gatcatgatg
     3661 acggcgctgt tactgaactg gcggcaggcg gcgggttaca cgattctggc cccgactgtg
     3721 gaggtggcag ccaacgcctt caatcctgcc agggatatgg tacgacggga cgatgatctg
     3781 gatgacctct gtcaggtgca gacacatatc cggaccatca cccaccgggt gacggacacc
     3841 accctgaagg tggtggcagc cgatccgaat acggtgtccg gtatcaagtc cgtggggacg
     3901 ctgattgatg agttgtggct gtttggcaag cagtacaagg cggaggacat gttacgtgaa
     3961 gccatcggcg ggcttgcctc ccgcccggaa gggtttgtgg tgtatacgac cacccagtcg
     4021 aatgaaccgc ccgccggggt gttcagacaa aaactgcagt acgcccggga tgtgcgcgac
     4081 ggcaaaatta atgatccgca ctttctgccg gtgatatttg aacaccctcc tgaaatggtg
     4141 gaaagcgggg ctcacctgct gatggaaaac ctcgccatgg tcattccgaa tctcggctat
     4201 tcagtggatg aggcctttct gtaccgggag taccgtaaag cccgggaagc cggtgaagag
     4261 acattccggg ggttcatgtc aaaacacgcc aatgtggaaa ttggtcttgc cctgcgctct
     4321 gaccgctggg cgggggctga tttctgggaa gagcagggcc gttgtatcaa cctggacgat
     4381 atcctgcgtc gtgttgatgt ggtgacggtg gggattgacg gcggagggct ggatgatctg
     4441 ctggggatgt atgtgattgg gcgtgaccgg gagacccgcg aatggctggg ctggggccat
     4501 gcctgggcgc atgaaaccgc ggtggtccga cggaagagcg aggcgtcccg gtttcatgat
     4561 cttgttgcct gtggagatat gaccattgtc cggcgtgtcg gggatgacac ggcggaagtg
     4621 gcggaatatg tgcgtcgcat tcatgaggct gagttactgg accatatcgg tattgacccg
     4681 tcaggggtgg ggcagattct ggattcactg gcggaagccg ggatccccga cggaattgtg
     4741 gtggggataa gccagggctg gaaactgggc ggggccatta aaaccaccga gcgcaaactg
     4801 gctgaagggg tgctggtgca tggtgaccag cccctgatgg cctggtgtgt cggcaatgcc
     4861 cgggtggagc ctaaaggtaa cgccattctt atcaccaaac aggccagtag acggggaaaa
     4921 attgacccgc tgatggcgct gttcaatgcg gtctccctga tgtcccttaa cccggaaccg
     4981 aaaaagaaag aatatgcggt ttttttcata taaccctgtt cacactgtaa ccatcacgaa
     5041 ccgctccggc ggttttttta ttttcaggag gctgatgtga ctcttaaacg ggcctgttcc
     5101 ctgctgacgg tgaaatcctt cagtgaggat gaacgggtga tcaccgggat tgcgtcaacg
     5161 ccttctccgg atcgggatgg tgacatcctg gagccggagg gcgcggagtt tggcagtgcg
     5221 atcccgtttc tctggcagca tgaccattcc cgcccggtgg ggcagtgtac ggtgcgccgg
     5281 gtcagcgaag ggctggaaat cacggcaaca ctggtgaagc ccgtaccgga tatgccgtcg
     5341 caactggctg cccggctgga tgaggtctgg gcggccatta agaccgggct ggtcaggggc
     5401 tgtccgtggg cttccgtccc catgaataca cctttctgga cggaggcgga ctgcattttc
     5461 tgcgctggga actgatggag gtgtctgccg tcaccgtgcc cgcgaatgcg gaatgcacca
     5521 tccggaccat taaatcttac gacagcccgt tttctgccgc gtccggcaac cggaaaccgg
     5581 tggtgaaaat cgcatcttct gccggcgctg cggcacagtc aacaaccgtt tttcataagg
     5641 aaaagaccat aatgaatatt ggcgaacaga ttaaaagttt tgaaaacaag cgtgtagcgc
     5701 tggcagcctc ccttgaggag gtcatgacca aagccgcaga ggaagggcgc acgctggatg
     5761 tggaggagga agagcattac gacaacaccg cagcggaaat ccgtcaggtg gatgcgcacc
     5821 tgaagcgcct gcgtgaactg gaagccggta aggccgccac ggcgcagccg gtgaaacagg
     5881 ccggtaacgg gaatgtggcc gcggtggctt ctgcgccggt gatccgtgtg gagcagaaac
     5941 tggataaggg gattggcttc gcccgctttg ccaaatcgct ggctgcggct aaaggcgtcc
     6001 gatctgaagc cctggaagtg gcccgtcgtc agtatccgga tgacagtcgt ctgcatcatg
     6061 tcctgaaatc ggcagtgggc gcggggacca ccacggatcc gcagtgggca ggcagcctgt
     6121 ctgaatatca ggaatacgca caggacttta ttgattacct gcgtccgcag accattatcg
     6181 ggcgatttgg tcagggcggg atccctgcac ttcgtcaggt gccgttcaat atccgtgtgc
     6241 acgcccaggt gtccggcggt gctgccggct gggtgggtga gggtaaggca aaacccctga
     6301 cgaagtttga ttttgaatcc atcaccttca gtcatgcgaa ggtgtcggcc attgcggtac
     6361 tgacggaaga gctgatccgt ttttccagtc cggcagctga tgcgctggtc cgtaatgcgc
     6421 tggcagaagc ggtggtggcg cgtctggaca cagactttgt ggacccgaaa aaagccgcag
     6481 tggcagatgt ctccccggcg tccatcaccc atgatgtgaa gggcaaggca tcaaccggta
     6541 acccggatgc ggatgcagag gcggcgtttg gacagtttgt ggcagcaaac ctgcagccca
     6601 ccggtgcggt ctggctgatg tccagcacca atgccctggc gctgtccatg cgtaaaaata
     6661 cgctgggtca gaaggaatac ccggacatga ccctgctggg ggggaccttc caggggctgc
     6721 cggtgattgt ctcccagtac gtgggtgacc agctggtgct ggtgaatgcc ccggatattt
     6781 atctggcgga tgacggcggc gtggcagtgg atatgtcccg cgaggcatca ctggaaatgc
     6841 agtctgagcc gaccggcgac agtaccacgc cgtcgccggt ggagctggtt tccatgttcc
     6901 agacaggcag tgtggccatc cgtgcggagc gctggatcaa ctggcgtcgt cgccgtaccg
     6961 cggcggtggc ggtgatcacc ggtgtgaact acggcagtgc gtccggcggc tgagtctgat
     7021 aaggaggacg ggaggcgagc gcctcccgta acaggttatg gcaaagatcc gatatctgca
     7081 gggcacgcat gatgcccggg ccggggatat ccgtgatgtg gcacagccgt gcgcggaggt
     7141 gctggttcgc ctgggaaagg cggagtacat cacggtgcga cgtccggcag gtcagaaaaa
     7201 gaaacgtgat gcggagcatg gcgaatgtgg aaccttttac agcgaacccg aaaaaaacag
     7261 aaatcaggac gtgacgtaag agaggcgggc tggaccagcc tgtttcaggc ggtggctgag
     7321 cccttttccg gcgcctggca gcagggcgtg aaagccgatc cggaagccgt cctctccttt
     7381 catgcggtgt tttcgtgcat ttcgctgata tcccaggata tcgccaaaat gcggctgcgt
     7441 cttatgcaga cggatgcgca ggggatacgc agggaaactc gacgggggga tattgcccgc
     7501 ctctgtcgtc gtcccaacgc ccagcagaac cgcatccagt tttttgaact gtggctgaac
     7561 gccaaactgc gtcacggcaa tacggtggtg ctgaaaatcc gtaactcccg ggggcagatc
     7621 aaagaactgc gcattctgga ctggagccgg gttgaaccac tggtggcgga tgacggcgaa
     7681 gtgttctacc gcatcacgcc ggaccggaac tgcgggatca cttaggcggt gacggtgcct
     7741 gcccgggaag tgatccacga ccggtttaac tgtttttttc atccgcttat agggttgccg
     7801 ccggtgtatg ccgccgggct ggccgccacg caggggcatc atattcagga aaattcgacg
     7861 tattttttca gaaatggcgg catgccgtcc ggggtgattg agatccccgg cagtattacg
     7921 gaagaaaatg cgaaaaaact gaagagcaac tgggacagcg ggtataccgg cgaaaatgcg
     7981 gggaaaacgg ccattctgag caacggggca aaatacaacc ccacgacgtt ttcaccggtg
     8041 gatgcgcaga cggtggaaca actgaagatg accgctgaaa ttgtctgttc ggtgttccgt
     8101 gtcccggcct acaagattgg cgtgggacaa ccgccttcca gtgacaacgt ggaggcgctg
     8161 gagcagcagt attattccca gtgcctgcag acgctgattg agtccattga actgttactg
     8221 gatgaggcgc tggaaacggg ggaaaacgag agtacagaat ttgatgtcac cacgctgctg
     8281 agaatggaca gtgagcggcg catgaaaacg ctgggggatg cggtgaaaaa tacgcttctc
     8341 acgcccaatg aggcccgtaa acgggagaac ctgccgcccc tggccggcgg tgatgcactg
     8401 tatcttcagc agcagaacta cagtctggag gcactgtccc gtcgtgatgc ccgtgaggat
     8461 ccgttcgcgt cgaccgggaa aacggcctta gcgcaaccgc ctgacggcgc atctgacggt
     8521 aataaggcaa tcagtgaaac agagcatgat gcggtgaaag cgatgttcag ggggattctg
     8581 aaaaaatgaa tgaacgtgaa ctgtccatta ttcgtgccct gggagaagaa ttcgccacgg
     8641 tgctggcgga tttacagcgc acatttgagg agaagatgag cgcgcaggca caagcgtttg
     8701 aagagaaact ggcttccctg tccgctctgt tacagaagca tgtgacggtg gatgaggtta
     8761 gccctgttct gcaggcgatg gtggatgacg cagtgggggc cattccgcta ccgcgtgatg
     8821 gtcacgatta tgatccggaa gtactgaagc aggcggtgaa tgatgcggtc gcgaaaatac
     8881 cggtgccggc agacggcaaa agtatcaccc ccgatgatgt gcgtccgatg cttgagcaga
     8941 tggtgaaaga ggctgtaagc catattcctg ttccgcgtga tggtcgtgac tacgatccgg
     9001 aagtactgaa gcaggcggtg aatgatgcgg tcgcaaatat tccgcagccg gcggacggta
     9061 aaagtctcac cccggatgat gtgcgtccga tgcttgaaca gatggtgacg gaggctgtaa
     9121 ggcatatccc tgttccgcgt gatggtcgtg actacgatcc ggatgttctg cagaaggcgg
     9181 ttctggatgc ggtgagtgcc ctgccggctc cgcaggacgg gcgtgatgcc acggctctgg
     9241 aaatactccc cgccattgac gttcaaaaat cctttccccg gggcacgtat gccacacacc
     9301 agggcggact ctggcgggcg tatgaaaaaa cgcacgggat gcggggatgg gaatgcctgg
     9361 ttgacggggt ggcggatatt gacgtcagca tgacgggtga gcggttgttc tctgtggtgg
     9421 tccggcagag cagtggccag cgtacggaaa aaacattttc cctgccggtg atgctctacc
     9481 gcggtgtgtt cagagccggt gaaacctacc accccggcga tacggtgacg tgggggggct
     9541 cgctgtggca ctgcaacagt atgaccgaag ataaacccgg agaagctcat tcatcagcct
     9601 ggaccctggc tgcaaaacgt gggcgggatg caggaggcgg aaaatgacgg cattactgac
     9661 actggaagag gtcaaggcac atctgcgtgt cgaccatgac gcggatgatg acatgctgat
     9721 ggacaaggtt cgtcaggcta ccgccgtgct gctggcctac attcagggca gccgggataa
     9781 agtgatccgt gaggacggtg aactgatccc gggcgaggca ttaacccgga tgaagggggc
     9841 tgccatgcga ctgaccggga tgctgtaccg gaatccggat cttgcggagc gggaagaact
     9901 gcttcagggg gagctgccgt tttctgtttc cgtgctgatt tacgatttgc gttgtccgac
     9961 ggtgttatga ggagggggaa tggcaatatc tgtaggtcgt ctgacacaga tgataagtgt
    10021 tctgaacccg gtgttaaccc gtaacgctgc cggagaaatg acggaagaat gggtgtcatg
    10081 cgggaaaatt catgcggata tccgaggcag gagcagccgg gagcggatgc agttcggtgc
    10141 ggaaatggcg caggcggaaa tccgcatctg ggtgcgcggt cagtccggtc gggaaatcac
    10201 ggcagcgtca cgacttcatg tgctgagtgg tccatggcgt gaccggatcc tgaacgttgt
    10261 cgggctgccc gtgccggatg cgaccggcgg gcgtctggaa attctctgtc ggctgggagg
    10321 ggaaaaatga tcgaaatcct gctggatttt tcggggctgg aggacatcag ccgcgatttg
    10381 cagcttctga gtggtgcgga aaataaccgg gtgctgcgtg aggcaacccg tgcgggtgcg
    10441 aatgtgctga aagaagaagt ggtgttacgg acaccggtac gcaggggaaa actgcgccgc
    10501 aatgtggtga tcctttcccg gcgctcccgc gatggcggga tggaatccgg tgtccatatc
    10561 cgtggtgtta atccggacac cggtaacagc gataacacca tgaaggcgga taacccgcgc
    10621 aatgctttct actggcggtt tgtggaaatg gggaccgtga atatgccacc gcacccgttt
    10681 gtgcgcccgg cgtttgatgt gcgcagtgaa caggcagctc aggtggcgat tgcgcggatg
    10741 aaccgggcca ttgatgaggt actgagacga tgacggaggc ggatttgtat cctcatctgg
    10801 cgcatcttgc cggcgggcag gtgtacccgt atgtggcccc cctgctggat ggcaggccgt
    10861 tggtggcgct tccgtgggtg gttttcagcc tgatttcatc ggtgtcagcg gacgtgatgg
    10921 gcgggcaggc ggagtcctca gtgtcggtgc agatagacgt ttatgccggg actgtgacgc
    10981 aggcgcgtca gatacgtcag gacgcccgtg aagccataat gctgctggcc ccgggatccg
    11041 tcagtgaaat gcaggactat attccggaaa accgctgtta ccgtgcaacc ctggagtttc
    11101 aggtcacggt gtgacttttt cttttttcta caaaaccata ccccgccgcg tgcgggtttt
    11161 ttattatcag gaggcagaat gtctgctttg tatgaacgct cacagctgac gcaggtgatg
    11221 atttcatctg ccctggcgac tgctgaaact atggataagg cggaatatct gcgcctggac
    11281 tgcaccatca aggaagtcca gttcaccgcc ggtcagaaac aggatattga tgtgaccacg
    11341 ctctgctcca cagagcagga gaacatcaac ggtctggggg cgtcgtctga gatttccatg
    11401 tcgggtaatt tttatctgaa tcaggcccag aacgccctgc gtgatgccta tgacaatgac
    11461 gcgttgtatg cgtttaaggt gctgtttccg tccggtaagg gctttaaat
//
LOCUS       DAJTMK010000129        11458 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTMK010000129 DAJTMK010000000
VERSION     DAJTMK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11458
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.131"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            106..882
                     /gene="exuR"
                     /locus_tag="OO135_002998"
     CDS             106..882
                     /gene="exuR"
                     /locus_tag="OO135_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5398762.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGQQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1227..1889
                     /locus_tag="OO135_002999"
     CDS             1227..1889
                     /locus_tag="OO135_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5398763.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1893..2276
                     /gene="mzrA"
                     /locus_tag="OO135_003000"
     CDS             1893..2276
                     /gene="mzrA"
                     /locus_tag="OO135_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5398764.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2423..2791
                     /locus_tag="OO135_003001"
     CDS             2423..2791
                     /locus_tag="OO135_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR5398765.1"
                     /translation="MKDRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2829..3134
                     /locus_tag="OO135_003002"
     CDS             2829..3134
                     /locus_tag="OO135_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5398766.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRAAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3137..3541
                     /locus_tag="OO135_003003"
     CDS             3137..3541
                     /locus_tag="OO135_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5398767.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3531..3830
                     /locus_tag="OO135_003004"
     CDS             3531..3830
                     /locus_tag="OO135_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5398768.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4016..4408
                     /locus_tag="OO135_003005"
     CDS             4016..4408
                     /locus_tag="OO135_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5398769.1"
                     /translation="MKKLEDVGVLVARILMPILFMTAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4478..5422
                     /gene="yqjG"
                     /locus_tag="OO135_003006"
     CDS             4478..5422
                     /gene="yqjG"
                     /locus_tag="OO135_003006"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5398770.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGITETVNFDHIRNHYFRSHKTINPTGIISIGPW"
     gene            5757..6122
                     /locus_tag="OO135_003007"
     CDS             5757..6122
                     /locus_tag="OO135_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5398771.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6364..6720
                     /locus_tag="OO135_003008"
     CDS             6364..6720
                     /locus_tag="OO135_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5398772.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6771..7667)
                     /gene="yhaJ"
                     /locus_tag="OO135_003009"
     CDS             complement(6771..7667)
                     /gene="yhaJ"
                     /locus_tag="OO135_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5398773.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     SDSMGEAKSWCLREIPKLFSGK"
     gene            7772..8473
                     /locus_tag="OO135_003010"
     CDS             7772..8473
                     /locus_tag="OO135_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5398774.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESTNFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8496..8660
                     /locus_tag="OO135_003011"
     CDS             8496..8660
                     /locus_tag="OO135_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398775.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8794..10104)
                     /locus_tag="OO135_003012"
     CDS             complement(8794..10104)
                     /locus_tag="OO135_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5398776.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALVAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10133..11454)
                     /locus_tag="OO135_003013"
                     /pseudo
     CDS             complement(10133..11454)
                     /locus_tag="OO135_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         2906 a         2985 c         2782 g         2785 t
ORIGIN      
        1 aattcaatac attaattcaa tcaccaaatt tatatatgac caatggcata cccatgaata
       61 aagtggtatg ataacttcgt ccgcattcca ctccgggaga cgcatatgga aatcactgaa
      121 ccacgccgtt tgtatcaaca acttgccgct gacctgaaag agcgcattga acagggcgtc
      181 tatctggtgg gtgataaact gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc
      241 cgaacggtag ttcgtgaagc cataatcatg ctggaagttg aaggctatgt ggaggtgcgt
      301 aaaggttcag gtattcatgt ggtttccaac cagccgcgcc atcagcaggc ggctgacaat
      361 aatatggaat tcgccaatta cggtccgttt gagttgcttc aggctcgcca gctcatcgaa
      421 agtaatattg ccgagttcgc ggcaactcag gtaacgaaac aagacatcat gaaactgatg
      481 gccattcagg aacaggcgcg cggccaacaa tgcttccgtg attccgagtg ggatttacag
      541 ttccacattc aggtagccct ggcgacgcag aactccgccc tggcggccat cgttgaaaaa
      601 atgtggaccc agcgtagcca taacccgtac tggaaaaaac tacacgaaca cattgattcc
      661 cgtaccgttg ataactggtg tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa
      721 gatccccatg ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta
      781 tttaacgaaa ccagcgacga tttcgagttc aatgccgacc gctatctatt cgccgaaaac
      841 ccggtagtgc atctcgatac ggccaccagt ggcagtaaat gaaactcctg tccgacaggc
      901 gctgcgacgc gcctgttcgc ttcgtcggta agcgaaaagt ataaagtgtc agcctgtgta
      961 aatcctctcg ccaccctccc ctgcattcag caaaatcaga ctccacggac atgtaatttt
     1021 gataacgaac aacgttgacc tttgttacaa ttagattcaa tttgaattta tgtttttgaa
     1081 tgctttctta tctcacgatt taacagggaa tagttcaggc tgtgttgatg tatcaaaccc
     1141 gcagaacata ccaaaacagt aataacattg cggtagtgca tcttttaaaa ccagcgtggc
     1201 gttaaccgat tcaccaggaa taatgaatgg aacttttgac ccaattgctg caagccctgt
     1261 gggcgcagga ttttgaaacc ctggccaatc catcgatgat tggcatgttg tattttgtct
     1321 tgtttgtaat tttgttcctt gaaaacggct tgcttccagc ggccttttta ccgggcgaca
     1381 gtttactggt attggtcggc gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga
     1441 ttctgctgct gaccgttgcc gccagcctcg gctgctgggt cagctatatt caggggcgat
     1501 ggctgggcaa tacccgcacc gtacaaaact ggctatctca tttacccgcg cattatcatc
     1561 aacgcgcaca ccatcttttt cataaacacg gtttatcggc gctgttaatt ggtcgcttta
     1621 ttgcgtttgt cagaacattg ctgccgacga ttgccgggtt atcagggctg aataacgcgc
     1681 gctttcagtt tttcaactgg atgagcggtc tgctgtgggt attgatcctg acaactctgg
     1741 gttacatgct cggcaaaacg ccggtatttt taaagtacga ggaccagctg atgtcatgcc
     1801 tgatgctgct cccggtggtg ctgctggtgt ttggcctggc aggttctctg gtcgtgttat
     1861 ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc
     1921 agctagcctg gtccggcact gttttacttc tggtcggcac gctgttgctg gcctggtccg
     1981 cggttcgcca gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc
     2041 cagacggttt ttcgatctgg catcaccttg acgctcatgg cattcctttc aaaagtatca
     2101 cccccaaaaa cgacactctg ttaattacat ttgactccag cgaccagagc gccgccgcaa
     2161 aagcggtcct cgacagaaca ttgccccatg gctacatcat tgcgcagcag gacaataaca
     2221 gtcaggctat gcagtggctg acccggttac gggataactc tcatcgcttc ggataacttc
     2281 ctgttatccg aaacatatca ctcactttgg tgatttcacc gtaactgtct atgattaatg
     2341 aggcggtggt taacactacc gcttcgttgt ctggacaatc gttcctttgt aataggtccg
     2401 aatcacaatg gaaggttcaa gaatgaaaga ccgcatcgct ttagctgttt ctctctttgc
     2461 tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa atatccttaa
     2521 ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc tgaataaagc
     2581 cctgagtgaa gtccgggcca actgttcaga tagccagctg cgtgccgatc atcacaagaa
     2641 aatcgcaaag cagaaagatg aggtggcgga acgccagcaa gatttagccg aggcgaagca
     2701 aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa ctggcagaag cgcaggaaga
     2761 gctgaaaaag ctggaagcgc gcgactacta actcacaata gtcactactt actcacctgg
     2821 agaaaactat gtcgaaagaa cacactacgg aacatctgcg tgctgagttg aaatcccttt
     2881 ccgatacgct ggaagaggtg cttagctcat ctggcgagaa gtcgaaagaa gagttgagta
     2941 agattcgtag caaagcggag caggcactga aacagagccg ttatcgcctg ggtgaaaccg
     3001 gtgatgccat cgccaaacaa acccgtgccg cggcggcgcg tgctgatgag tatgtgcgtg
     3061 aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc ggcgttctgc
     3121 tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa gggcccggta aaagcgttct
     3181 gggcatcggg cagcgaattg tttccatcat ggttgaaatg gtggaaacac gtctgcggct
     3241 ggcggtggtg gagctggaag aggaaaaagc taatctcttt caacttttac tgatgctggg
     3301 cctgacgatg cttttcgctg catttggtct tatgagcctg atggtgctaa ttatttgggc
     3361 ggttgatccg caatatcgcc tgaatgcgat gattgccacc accgtggtgt tgctgctact
     3421 ggcactgatt ggcggtatct ggacgttacg taaatcgcgt aaatccacct tgctgcgcca
     3481 tacacgccat gagctggcaa acgatcggca actgctcgag gaggagtccc gtgagcagta
     3541 aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa caacggctgg
     3601 atctttccgc cagtcgtcgt gaatggctgg aggcaacagg cgcttacgat cgtcgctgga
     3661 atatgctgct aagtctgcgc tcctgggcgc tggttggcag tagcgtgatg gcgatctgga
     3721 cgattcgcca tcctaatatg ctggtccgct gggccagacg cggttttggc gtatggagcg
     3781 cctggcgtct ggttaaaacg accctcaagc agcaacagct tcgcggttaa cttccctctg
     3841 gccggagcca ttccggcctt atccctcaaa ttttttgaag atttttgaca gttttccttg
     3901 ctaacaatca tcatttacca cgtttatgat tctctccatc gacagcaacg acgctaatac
     3961 cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc gcagtggaga gtatgatgaa
     4021 aaaattagaa gatgttggtg tactggtagc gcgcatttta atgccgattc tgtttatgac
     4081 cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa caatatatgg aagcaatggg
     4141 tgtcccgggt tttatgctgc cgctggtgat tctgcttgag ttcggtggtg gtctggcaat
     4201 cctgttcggt ttcctgactc gcaccacagc cctgtttact gcgggcttta cgctgctgac
     4261 ggcattttta tttcacagca agtttgctga aggcgtcaac tcgctgatgt tcatgaaaaa
     4321 cctgacaatt tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat
     4381 cgaccgcctg ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg
     4441 aggaagctcc tcgtttttgc tattggagga gagaaaaatg gggcaactga ttgacggcgt
     4501 ctggcatgac acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc
     4561 cgcatttcgt aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt
     4621 tatcgcagag aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg
     4681 cacgctgatc atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa
     4741 cccgctgatg ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga
     4801 tacgctctat caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag
     4861 cggacgagtt actgttcccg tgctgtggga caaaaagaac cacaccattg tcagcaacga
     4921 atcagcagaa atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg
     4981 tgattactac ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga
     5041 caccgttaac aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga
     5101 ggcggtggcg aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg
     5161 ttacctgacc ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cactggtgcg
     5221 ttttgatcca gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct
     5281 gaatctgtat ggcttcctgc gcgatatcta ccagatgccg gggatcaccg aaactgtcaa
     5341 tttcgatcac atccgtaatc attacttccg cagccataag accatcaacc ctacggggat
     5401 tatttcaatt ggtccgtggt aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg
     5461 ttaaaaaaag gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt
     5521 cggatgcggc gtgaacgcct tatcaaacct acaaaacgtt tatattcaat atgttaaaat
     5581 gaccgcgtag gccagataag cgtagcgcat ctggcgattt tgcgcttgtc atcagtctta
     5641 gcgcaccttc acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac
     5701 gtctattctt aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg
     5761 actggtatct gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag
     5821 agtactggat gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata
     5881 aaatgttagg ctggcaacgc gccggtggcg aaggcatcct gacgacaatc tacggtattc
     5941 tggtgttttt accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg
     6001 cgtggtgggc actgttgttc ttaatcccgt ttatcggctg gcttattatc atcgtcttta
     6061 actgccaggc gggtacgcca ggcgaaaacc gctttggacc agatccgaaa ctggaacctt
     6121 aaaacaaatc tgctcgccgg atgtctgact catccggctt ttatcatcat gttgatgaat
     6181 gaataattta ctaatgttta tttaaaatat ttcaatgtcg ttatataaat tacctataaa
     6241 aaacaaccat gtatttatct taaatatcaa aacctttata acatccgaaa aagaaagata
     6301 cgaatagcat ttacacaata cccggaatag actttcgtca cttttaatta aagggatgtt
     6361 tttatgcagt ggtatttagc cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt
     6421 cgtaaagagt actggatgtt tactctgatt aacgccatcg tcggcgctat tatcaatgtc
     6481 attcaattga ttttaggttt agagtttcca ttcctttctc tcatttacct ggcagcaaca
     6541 atcattccgg tcattgctct gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgcg
     6601 tgggcgcttc tctatctggt accgatcatt ggctggttag ttctgtttgt atttgcctgt
     6661 ttagaaggca attctggcag caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa
     6721 tcttagaatt ggggcgatat ttcgcccctt tttattaaca ataataaata ttattttccg
     6781 ctaaaaagtt tgggaatttc ccgcagacac caggatttcg cttcccccat actgtcacta
     6841 cgccaggcca taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc
     6901 ccttccgcaa tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc
     6961 agtaatgcct gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc
     7021 agctgtacgg tcaacaccgg gcgctcacga gcggtatccg ctaccgcaat tccacgatat
     7081 ttcacgcgcg tcacttcaga taacggttcc ggctcctgat gaatcgggtg atcaggcgcg
     7141 gcaacgtaga cgttcattaa ggtatagagc ttgcgcgagt tgatctccga cgaggaacga
     7201 aaatgcatat ccggcgcgat aacgatatcc gcccgcccct gctccagtcg ttcccacgcc
     7261 cccgccagca cttcggtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg
     7321 attaacggga aaaaggcagg tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt
     7381 tcccaaccgc gcgcgagggc ttccgcatcg gtagtcagtt tatctgctgc ttccagcaaa
     7441 acgcgccctc gctccagcaa catcctcccg acattggtga atttggtgcg atggcccgag
     7501 cggtcaaaca gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg
     7561 gaaggcacgc gtcccagctc atccgccgcc gccgcaaaac tgccccggcg atcgatcgca
     7621 tccataaccc gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc
     7681 aggaaatttg aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac
     7741 cataaaagga agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg
     7801 tggacaagca gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt
     7861 cgacccgaaa ttgttaggct atgcctccct gcgtgtgctt aaccaggaag tgctggcccc
     7921 aggtgccgcc tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga
     7981 tggagaagca gagtatcgcg acagcgaagg caatcatgtt caggccagcg ccggtgaggc
     8041 gttgctgctc tctacccagc cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa
     8101 accgttaaca cgaatgcagc tttggctgga cgcctgcccg cagcgagaga atccgctgat
     8161 tcaaaaactg gcgcttaata tgggcaagca gcaattaatc gcctcgccag agggtacgat
     8221 gggaagcctg caattacgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga
     8281 aagtacgaat ttccagttgc atggaccacg cgcgtatttg caatcgattc acgggaaatt
     8341 tcatgcgctt acgcatcatg aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat
     8401 tcgtgacgag gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga
     8461 tttgcctgtc tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc
     8521 gccagccagt gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa aattttggct
     8581 atgaagagat gttgagcgag ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg
     8641 aggatgaagc taccgcgtaa agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc
     8701 tacaaaatcc aacaacatta acgaattaaa caacccgtag gccggataag gtgcgccagc
     8761 atcgcatccg gcacaatact caaaacctgg cgtttatctg gccttgctcg ccataatctc
     8821 gataatctgc cgatccgttt gctgcatcga atggcttgct aacgcacaca ggttggcaat
     8881 cgactgctca acatcatgcg ccacgatccc ttcattgccg gtcacggcgg tatcatccag
     8941 cgccattaac accgctttcc acgcagccga agcactggtc gaaaccttca tcgcgcagct
     9001 gttcgacgca ccatcgcaaa tcatgccgct gacatcgccg atcatactgc tgatcgccat
     9061 cgagatagtt tcataacgcc catccaccag ccatgccatc ccggcggcgg cccccattgc
     9121 tgcggtcgtt gcggcacaaa gtgcagacaa acgcggtaac tggttatgga tgtaaattgc
     9181 gctcaaatga gaaagcatca gcgcacgcgc cagtcgttca tcatccgctc cgaagtgttc
     9241 tgctacaacc accacaggca ttgttgcggt gatcccctgg ttacccgagc cggagttact
     9301 catagccgga agcgtagcgc cgcccatacg cgcatcggat gccgcgctgg tacgaatcac
     9361 aatggatgaa gagagatctt tcgccagcaa accgcgctcg cactgttttt ccagcgtcgc
     9421 gccaatatgc agcccccact taccgctcaa accttcctgc gataacgcac aatttagctt
     9481 cgcggaatcg agaataaagc ggatcgccgc aaacgggact tcattgacga acttcaggat
     9541 ctcagccagc gtcgttctgg aaagcaccgt cagcggagac tcttgctcgc cctctgccac
     9601 acacgcctgc tgggtaaaca ccacactatt gtgcgtctca atatgcacaa tgttggtatg
     9661 cccgccgacg atggtgacac acgcccactt ctcaccgttc cagactttgg cgcgtgagaa
     9721 gaggatttca ttgcaaggtt cctggatctt aacggagact ttccccgccg ccagcagtgc
     9781 tttggcatcg gcaattgcct gcgcagttgc gtctttcagc acttccagcc cggcgttggc
     9841 atttccacct aacgccccca gcgccgccgc aatcggcagc cccaccattc ccgtgccggg
     9901 aacggtgacg cccagaccgt tcttcatcag atttggcgaa acccaggctt ctacacgttc
     9961 aaccggacct tccagttctg ccgcagcaac cgccgccacc agcgccagtg aaatcggttc
    10021 agtacatccc agcgccggtt ttacttcctc ctgaacggcg aggatgtaac gctgccataa
    10081 cggatttaaa gtcgaatcaa acatattaaa aaaccttaaa atttcaggta aatcaagaaa
    10141 atgccaggaa cggagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca
    10201 tccctttgta tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc
    10261 ctaccatgcc gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta
    10321 cgatggcgct ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt
    10381 cgttaatctt ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg
    10441 cttcgcgaaa gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa
    10501 tgacgctgac cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg
    10561 ccagcgcgga aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg
    10621 tgaacgacac ggcgtagaaa aagacggtga caaataaaat gccaaacgcg atattcattg
    10681 cccgcaatgc tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca
    10741 ccatcgggct taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa
    10801 tggcgttttt caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca
    10861 ttcccaccat cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgccgg
    10921 tcgaaatttt gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca
    10981 gtaccagacc ataaaaggga ctgtctgaca gcaacccttc cgtcacaccg aaggtatgca
    11041 gataggaagc actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca
    11101 ttacgaaata gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga
    11161 cactcgggta atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa
    11221 acagatacat tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca
    11281 aaccgacctg caccgggaga aaaacacaat cgccatcccg atactcataa tcacccagcc
    11341 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    11401 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatt
//
LOCUS       DAJTMK010000130        11108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTMK010000130 DAJTMK010000000
VERSION     DAJTMK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.132"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..548
                     /gene="hdhA"
                     /locus_tag="OO135_003014"
     CDS             <1..548
                     /gene="hdhA"
                     /locus_tag="OO135_003014"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5398777.1"
                     /translation="LSALADFAISKLGKVDILVNNGGGGGPKPFDMPMADFRRAYELN
                     VFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHLVRNMAFD
                     LGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANAALFLCSP
                     AASWVSGQILTVSGGGVQELN"
     gene            777..1367
                     /gene="uidR"
                     /locus_tag="OO135_003015"
     CDS             777..1367
                     /gene="uidR"
                     /locus_tag="OO135_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5398778.1"
                     /translation="MMDNMQTEAQPTRPRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1755..3566
                     /gene="uidA"
                     /locus_tag="OO135_003016"
     CDS             1755..3566
                     /gene="uidA"
                     /locus_tag="OO135_003016"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5398779.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWVGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNAWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3563..4936
                     /gene="uidB"
                     /locus_tag="OO135_003017"
     CDS             3563..4936
                     /gene="uidB"
                     /locus_tag="OO135_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5398780.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNIFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            4975..6240
                     /gene="uidC"
                     /locus_tag="OO135_003018"
     CDS             4975..6240
                     /gene="uidC"
                     /locus_tag="OO135_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5398781.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRIGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSSGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6466..7974)
                     /locus_tag="OO135_003019"
     CDS             complement(6466..7974)
                     /locus_tag="OO135_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5398782.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8075..9250)
                     /gene="manA"
                     /locus_tag="OO135_003020"
     CDS             complement(8075..9250)
                     /gene="manA"
                     /locus_tag="OO135_003020"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5398783.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTHFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9449..11095
                     /gene="fumB"
                     /locus_tag="OO135_003021"
     CDS             9449..11095
                     /gene="fumB"
                     /locus_tag="OO135_003021"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5398784.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2768 a         2602 c         2867 g         2871 t
ORIGIN      
        1 aactctctgc actggcagac tttgctatca gtaagctggg taaagttgat attctggtta
       61 acaacggcgg tggcggtgga cctaaaccgt ttgatatgcc aatggcggat tttcgccgtg
      121 cttatgaact gaatgtgttt tcttttttcc atctgtcaca acttgttgcg ccagaaatgg
      181 aaaaaaatgg cggtggcgtt attctgacca tcacttctat ggcggcagaa aataaaaata
      241 taaacatgac ttcctatgca tcatctaaag ctgcggccag tcatctggtc agaaatatgg
      301 catttgacct gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg gcaatattaa
      361 ccgatgccct gaaatccgtt attacaccag aaattgaaca aaaaatgtta cagcacacgc
      421 cgatcagacg tctgggtcaa ccgcaagata ttgctaacgc ggcgctgttc ctttgctcgc
      481 ctgcagccag ctgggtaagc ggacaaattc tcaccgtctc cggtggtggg gtacaggagc
      541 tcaattaata cactaacgaa ccggtaaaca gccgtatgtg ttgtttaccg ggatagcgtg
      601 tgcaattcat caacgtctct gctaaacaac tggcagccaa atcacggcta ttggttaacc
      661 aatagctgga tgaaaagcat acgaatagag tgtgccttcg cactattcaa cagcaatgat
      721 aggcgctcac ctgacaacgc ggtaaactag ttattcacgc taactataat ggtttaatga
      781 tggataacat gcagactgaa gcacaaccga cacggccccg gatcctcaat gctgccagag
      841 agattttttc agaaaatgga tttcacagtg cctcgatgaa agccatctgt aaatcctgcg
      901 ccattagtcc cgggacgctt tatcaccatt tcatctccaa agaagccttg attcaggcga
      961 ttatcttaca ggaccaggag agggcgctgg cccgtttccg ggaaccgatt gaagggattc
     1021 attttgttga ctatatggtc gagtctattg tctctctcac ccatgaagcc tttgggcaac
     1081 gggcgctggt ggtcgaaatt atggcggaag ggatgcgtaa cccacaggtc gccgccatgc
     1141 ttaaaaataa gcatatgacg atcactgaat ttgttgccca gcggatgcgt gatgcccagc
     1201 aaaaaggcga gataagccca gacatcaaca cgtcaatgac ttcacgttta ctgctggatc
     1261 tgacctacgg tgtactggcc gatatcgaag cggaagatct ggcgcgcgaa gcgtcgtttg
     1321 ctcagggatt acgcgcgatg attggcggta tcttaaccgc atcctgatcc tctcatttcg
     1381 cgggctggtg ataactgtgc ccgcgtttca tactttaatt tccctgtaca aaatacaatc
     1441 tagccgggat cggagaattc cccccaaaaa tattcaccgt agccatatgt catgagagtt
     1501 tatcgttctc aagatgcccg aacgaacgtt cgattgctta ttttatggct tctgtcaacg
     1561 ctgttttaaa gattaatgcg atctatatca cgctgtgcgt attgcagatt ttggtttttt
     1621 gatcgcggtg tcagttcttt ttatttccat ttctcttcca tgggtttctc acagataact
     1681 gtgtgcaaca cagaattggt taaccagtca gattaaaggt tgaccagtat ttttatttga
     1741 atgaggagtc ccttatgtta cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg
     1801 acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg
     1861 aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg
     1921 ccgatgcaga tattcgtaat tatgtgggca acgtctggta tcagcgcgaa gtctttatac
     1981 cgaaaggttg ggtaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca
     2041 aagtgtgggt caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag
     2101 ccgatgtcac gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca
     2161 acgaactgaa ctggcagact atcccgccgg gaatggtgat taccgatgaa aacggcaaga
     2221 aaaagcagtc ttacttccat gatttcttta actatgccgg gatccatcgc agcgtaatgg
     2281 tctacaccac gccgaacgcc tgggtggacg atatcaccgt ggtgacgcat gttgcgcaag
     2341 actgtaacca cgcgtctgtt gactggcagg tggtagcaaa tggtgatgtc agcgttgaac
     2401 tgcgtgatgc ggatcagcag gtggttgcaa ctggacaagg taccagcggg actttgcaag
     2461 tggtgaatcc ccacctctgg caaccgggtg aaggttatct ctatgaactg tgcgtcacag
     2521 ccaaaagcca gacagagtgt gatatctacc cgctgcgcgt cggcatccgg tcagtggcag
     2581 tgaaggacga acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc
     2641 atgaagatgc ggatttgcga ggcaaaggat tcgataacgt gctgatggtg cacgatcacg
     2701 cattaatgga ctggattggg gccaactcct accgaacctc gcattaccct tacgctgaag
     2761 agatgctcga gtgggcagat gaacatggca tcgtggtgat tgatgaaact gcagctgtcg
     2821 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     2881 gcgaagaggc tgtcaacggg gaaactcagc aggcgcactt acaggcgatt aaagagctga
     2941 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     3001 cccgtccgca aggtgcacga gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     3061 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     3121 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     3181 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac
     3241 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     3301 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     3361 tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     3421 cctcgcaagg catattgcgc gttggcggta acaagaaggg gatcttcacc cgcgaccgca
     3481 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     3541 aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcacca tcgtcggcta
     3601 cagcctcggt gacgtcgcca ataacttcgc cttcgcaatg ggggcgctct tcctgttgag
     3661 ttactacacc gacgtcgctg gcgtcggtgc cgctgcggcg ggcaccatgc tgttactggt
     3721 gcgggtattc gatgccttcg ccgacgtctt tgccggacga gtggtggaca gtgtgaatac
     3781 ccgctgggga aaattccgcc cgtttttact cttcggtact gcgccgttaa tgatcttcag
     3841 cgtgctggta ttctgggtac cgaccgactg gagccatagc agcaaagtag tgtatgcata
     3901 tttgacctac atgggcctcg ggctttgcta tagcctggtg aatattcctt atggttcact
     3961 tgctaccgcg atgacccaac aaccacaatc ccgcgcccgt ctgggcgcgg ctcgtgggat
     4021 tgccgcttca ttgacctttg tctgcctggc atttctgata gggccgagca ttaagaactc
     4081 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg cgattgccgg
     4141 aatggtgctt tacttcatct gcttcaaatc gacgcgtgag aatgtggtac gtatcgttgc
     4201 gcagccgtca ttgaagatca gtctgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     4261 gtgcatcggt gcgctgtgtg tgctgatttc gacctttgcg gtcagcgcct cgtcgttgtt
     4321 ctacgagcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     4381 cttggttggt actgtggcat cggcaccgct ggtgccgggg atggtcgcga ggagcggtaa
     4441 aaagaatatc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     4501 ggtttccgtc tggtcactac cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     4561 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     4621 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     4681 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     4741 caatcaggtg caaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     4801 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4861 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4921 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4981 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttat
     5041 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     5101 agggcggata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     5161 ttgctcgatt tcaatagtgg ttattattcc aatattattg gcgttgaagg cggggcatat
     5221 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     5281 aaaagttttg gctttgccct gggggcagta aaaataaaac cctgtgaaaa tagcctgctt
     5341 aaattaggtc gcatcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     5401 atggctggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     5461 gctttaacac caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     5521 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     5581 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     5641 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     5701 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     5761 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     5821 acctggaatg cgccgtgggg aagcgtattt ggcagtagtg gttatttgcg ccatgcaatg
     5881 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5941 cgtgaaggaa tgcagtcctg gcaattgggc gtcaactatc gtttaacgcc gcaatttaca
     6001 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     6061 gaaggcacgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     6121 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     6181 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     6241 gaaataacgc cggagagaaa aacctcaggc gtttcagatt gttgacaaag tgcgcgtttt
     6301 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     6361 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     6421 gatgaaaacg ccgaagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     6481 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     6541 cgacattttc tgcccattta acgttatctg accgttagta tattgcaggc tggtggtgat
     6601 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     6661 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     6721 agtcataaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     6781 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6841 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6901 gacagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6961 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     7021 agtttgcgcg ttatattgct ggctgaactg atgccaggct tcaccatcaa tctggccgac
     7081 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     7141 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     7201 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     7261 tgacagtttt tggtttccta cgcgctcacc aaaacttgcc agcgtgctgg aaccgtcggt
     7321 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     7381 accactttgc gcctccccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     7441 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggcttgag
     7501 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     7561 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     7621 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     7681 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     7741 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     7801 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7861 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7921 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7981 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     8041 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     8101 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     8161 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     8221 acaaaatggc ggcactctgc tggctaatgg tggtttcttt atcactaagg tcatgcagcg
     8281 agaaggcaaa atcatccact ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     8341 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     8401 tatcaatgta cttaggcgtc agacccgcac gcagcacgtt atcggagttc gccatcactt
     8461 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     8521 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     8581 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     8641 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggctggcga
     8701 acagttcgct taaacgttcg gcattaggct gttgtaaaaa gtgagcaatt gccggatgtg
     8761 cacctgcgac tggctggagt agggagacaa tctcggaaaa ctcacgaaac gcgttcatcg
     8821 caaggaaatg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8881 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8941 tgcgtttgtt tggatgaacc tgaatggaga gcggctgtgc tgcgcataat actttgaaca
     9001 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     9061 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     9121 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     9181 cataaagttc agtcaacgcc gttttgctgc cccaggcata gttttgcact gagttaatga
     9241 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccactatt aaagcaagaa
     9301 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     9361 agtgtgtagg atattgttac tcgcttttta cagggcaacg gaacacccgc ccagagcata
     9421 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcaggctcc
     9481 ttttccactc aaaaaagatg atactgagta ttacctgcta accagcgaac acgttagcgt
     9541 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     9601 gcgtcaggcg tttcatgatg cgtcatttat gctgcgtccg gctcaccaac aacaggtggc
     9661 cgacattctg cgtgacccgg aagccagcga aaatgataaa tatgtggcgc tgcaattcct
     9721 gcgcaactcc gacatcgcgg cgaaaggtgt tctgccaacc tgtcaggata ccggcactgc
     9781 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggctgtgatg aagcggcgct
     9841 ggcgcgcggt gtctataaca cttatatcga agataatttg cgctactcgc aaaacgcgcc
     9901 gctggatatg tataaagagg tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9961 tgccgttgat ggcgacgagt acaaattcct ctgtatcgcc aaaggtggtg gttcggcaaa
    10021 caagacgtat ctctatcagg aaaccaaagc gttactaacg ccggggaaac tgaaaaatta
    10081 cctggttgag aagatgcgca cgctgggtac ggcggcctgt cctccgtatc atattgcgtt
    10141 cgtaattggt ggaacttctg cagaaactaa ccttaaaaca gtgaaactgg cttccgctaa
    10201 atactatgac gaactgccaa cggaagggaa tgagcacggt caggcattcc gcgatgtgga
    10261 actggaaaaa gaattgctga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
    10321 taaatacttc gctcacgaca tacgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
    10381 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
    10441 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
    10501 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
    10561 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
    10621 cggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
    10681 gcagtacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
    10741 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
    10801 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10861 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10921 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10981 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    11041 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    11101 gccgccct
//
LOCUS       DAJTMK010000131        11047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTMK010000131 DAJTMK010000000
VERSION     DAJTMK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.133"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1073
                     /locus_tag="OO135_003022"
     CDS             <1..1073
                     /locus_tag="OO135_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5398785.1"
                     /translation="IEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVI
                     RYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPT
                     PFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
     gene            1098..1325
                     /locus_tag="OO135_003023"
     CDS             1098..1325
                     /locus_tag="OO135_003023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398786.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            1921..3489
                     /gene="cydA"
                     /locus_tag="OO135_003024"
     CDS             1921..3489
                     /gene="cydA"
                     /locus_tag="OO135_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5398787.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            3505..4644
                     /gene="cydB"
                     /locus_tag="OO135_003025"
     CDS             3505..4644
                     /gene="cydB"
                     /locus_tag="OO135_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5398788.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            4659..4772
                     /gene="cydX"
                     /locus_tag="OO135_003026"
     CDS             4659..4772
                     /gene="cydX"
                     /locus_tag="OO135_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5398789.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            4772..5065
                     /gene="ybgE"
                     /locus_tag="OO135_003027"
     CDS             4772..5065
                     /gene="ybgE"
                     /locus_tag="OO135_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5398790.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            5215..5619
                     /gene="ybgC"
                     /locus_tag="OO135_003028"
     CDS             5215..5619
                     /gene="ybgC"
                     /locus_tag="OO135_003028"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5398791.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERIAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            5616..6308
                     /gene="tolQ"
                     /locus_tag="OO135_003029"
     CDS             5616..6308
                     /gene="tolQ"
                     /locus_tag="OO135_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5398792.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            6312..6740
                     /gene="tolR"
                     /locus_tag="OO135_003030"
     CDS             6312..6740
                     /gene="tolR"
                     /locus_tag="OO135_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5398793.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            6805..7959
                     /gene="tolA"
                     /locus_tag="OO135_003031"
     CDS             6805..7959
                     /gene="tolA"
                     /locus_tag="OO135_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5398794.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAADK
                     KAAADKKAAEKAAAEKAAADKKAAAEKAAAAAEKAAAEADDIFGELSSGKNAPKTGGG
                     AKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAP
                     DGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            8092..9384
                     /gene="tolB"
                     /locus_tag="OO135_003032"
     CDS             8092..9384
                     /gene="tolB"
                     /locus_tag="OO135_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5398795.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQVRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            9419..9940
                     /gene="pal"
                     /locus_tag="OO135_003033"
     CDS             9419..9940
                     /gene="pal"
                     /locus_tag="OO135_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5398796.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            9950..10741
                     /gene="cpoB"
                     /locus_tag="OO135_003034"
     CDS             9950..10741
                     /gene="cpoB"
                     /locus_tag="OO135_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5398797.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            10906..10981
                     /locus_tag="OO135_003035"
     tRNA            10906..10981
                     /locus_tag="OO135_003035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10939..10941,aa:Lys,seq:ttt)
BASE COUNT         2736 a         2584 c         2948 g         2779 t
ORIGIN      
        1 aaattgaaga aagctctgat gatggtgatg aatatgacta ttcacccgca aaagaagagt
       61 gggtaattac cgcagcgaac gcgaaaccgc aatgcgatat tattcatgaa gcctggcaga
      121 gcagggctgt tatccgctat gacatggcag tgccgctcaa tttgtcagaa cgcagcgccc
      181 ggcaatccac tggcagagta ggggtggtgt tggttgtcac tcttagtcat aacagcaggc
      241 gtattgatgt ggatatcaac cttgataacc aggctgacga tcatcgcctt cgtgtcctgg
      301 tccctacacc ttttaacacc gacagtgttc tggcagatac gcagtttggt tcgctaacgc
      361 gccccgtgaa cgacagtgca atgaacaact ggcagcaaga aggctggaaa gaagcgccgg
      421 ttccggtatg gaatatgctc aactatgttg ccttacagga agggcgtaac ggcatggctg
      481 tctttagcga agggttacgt gaatttgaag tcatcggtga agagaagaaa acctttgcca
      541 ttacgttgct gcgtggcgtg ggcttactgg gcaaagaaga tctgctttta aggcctgggc
      601 ggccttcggg aattaaaatg ccagtcccgg actcacaact acgtggtctg ctttcttgtc
      661 gcctaagttt attgagttat accggtacgc caaccgccgc tggtgtagct cagcaggcgc
      721 gagcatggct gactccagta cagtgttaca acaaaatccc atgggatgcg atgaagctca
      781 acaaagccgg attcaacgtg ccggaaagtt atagtttgtt gaaaatgccc ccagtgggat
      841 gcctgataag cgcacttaag aaagctgaag accgacaaga agtgatttta cggctgttta
      901 atccggctga atcagcaacc tgtgatgcga ctgttgcttt cagtcgcgag gtgatttctt
      961 gctcagaaac gatgatggat gaacacatta ccaccgagga aaatcaaggt tcaaatctat
     1021 cggggccttt tttacccggc cagtcacgga cgttcagtta ccggcttgcc tgaatagcaa
     1081 tcaaaccgaa gccacatatg cgcggccaga ttgttgacaa agggcgcttt gttcatgccg
     1141 gatacggcat gaatgcttta ttcggtctac aaaagcaggc aaattcaata tattgcagag
     1201 atgatgtaga cactggcaag cgtagcgcat caggtaattt tgcgtttatc ttcactctca
     1261 agccacgtat atgtggcttt atttttaaca aaataataac cctgggtgag ttaattataa
     1321 tataattata agttaattaa atgttaatat cggcgggggt ggatttatgc ctttattagt
     1381 aatcctgaaa ctctgcgtcg tattagccag tgaccaaaaa aagaattaag gtcaaccgtg
     1441 ctgtttttgc ttcgtctctt tttatcttta attgccaacc gaaactaatt tcagccttat
     1501 aactcacaca ttttaaacat aaatgtcact aaagttacct tattgaaaca tgattaacat
     1561 aatttgtagg aattgatatt tatcaatgta taagtcttgg aaatgggcat caaaaagaga
     1621 taaattgttc tcgatcaaat tggctgaaag gcggtaattt agctataaat tgatcaccgt
     1681 cgaaaaatgc aaatttgctt caacaaaaac ctgtttattg taaggatttt gcggcgtaat
     1741 atatacgtgg gatcaatttg agtttttatt aacatgtttg caacctttct ttacgccgtt
     1801 tttgtgtgca ttcacatggt atgatgaaag tgttcaaaca aatttctatt ggggcatgcg
     1861 tgtgaccctt tctaacgggg ttcactctcg gagtcttcat gcgatgagca aggagtcatg
     1921 atgttagata tagtcgaact gtcgcgctta cagtttgcct tgaccgcgat gtaccacttc
     1981 ctttttgtgc cactgacgct cggtatggcg ttcctgctgg ccattatgga aacggtctac
     2041 gtcctctccg gcaaacagat ttataaagat atgaccaagt tctggggcaa gttgtttggt
     2101 atcaacttcg ctctgggtgt ggctaccggt ctgaccatgg agttccagtt cgggactaac
     2161 tggtcttact attcccacta tgtaggggat atcttcggtg cgccgctggc aatcgaaggt
     2221 ctgatggcct tcttcctcga atccaccttt gtaggtctgt tcttcttcgg ttgggatcgt
     2281 ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg cgctcggttc aaacctgtcc
     2341 gcactgtgga ttctggttgc gaacggctgg atgcaaaacc caatcgcgtc cgatttcaac
     2401 tttgaaacta tgcgtatgga gatggtgagc ttctccgagc tggtgcttaa cccggttgct
     2461 caggtgaaat tcgttcacac tgtagcgtct ggttatgtga ctggcgcgat gttcatcctc
     2521 ggtatcagcg catggtatat gctgaaaggt cgtgacttcg ccttcgctaa acgctccttt
     2581 gctatcgctg ccagcttcgg tatggctgct gttctgtctg ttattgttct gggtgatgaa
     2641 tccggctacg aaatgggcga cgtgcagaaa accaaactgg ctgctattga agccgagtgg
     2701 gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca ttcctgatca ggaagaggag
     2761 acgaacaaat ttgcgattca gatcccttac gcactgggca tcattgcaac gcgttccgtg
     2821 gataccccgg ttatcggcct gaaagagcta atggtgcagc atgaagaacg cattcgtaac
     2881 gggatgaagg cgtactctct gctcgaacaa ctgcgttctg gttctaccga ccaggcggtt
     2941 cgtgaccagt tcaatagcat gaagaaagac ctcggttacg gtctgctgct gaaacgctat
     3001 acgccaaacg tggctgatgc gactgaagcg cagattcaac aggcaaccaa agactccatt
     3061 ccgcgtgtag cgccgctgta cttcgcgttc cgtattatgg tggcgtgtgg cttcctgctg
     3121 ctggcaatca tcgcgctctc tttctggagt gtcatccgca accgcattgg cgagaaaaaa
     3181 tggctgctgc gcgccgcgct gtacggtatt ccgctgccgt ggattgctgt agaagcgggc
     3241 tggttcgtgg ctgaatatgg ccgccaaccg tgggctatcg gtgaagtgct gccgacagct
     3301 gtggcgaatt cgtcactgac cgcaggcgat ctcatcttct caatggtgct gatttgcggc
     3361 ctgtataccc tgttcctggt ggcagaattg ttcttaatgt tcaaatttgc acgcctcggc
     3421 ccaagcagcc tgaaaaccgg tcgctatcac tttgagcagt cttccacgac tactcagccg
     3481 gcacgctaag acaggagtcg tcaaatgatc gattatgaag tattgcgttt tatctggtgg
     3541 ctgctggttg gcgttctgct gattggtttt gcagtcactg acggtttcga catgggggtg
     3601 ggcatgctca cccgtttcct cggtcgtaac gacaccgagc gtcgaattat gattaactcc
     3661 attgcaccac actgggacgg taaccaggtt tggctgatca ccgcgggcgg cgcactcttt
     3721 gctgcctggc cgatggtcta tgccgctgcg ttctccggct tctatgtggc gatgatcctc
     3781 gtgctggcgt ctttgttctt ccgtccggtc ggttttgact accgctccaa gattgaagaa
     3841 acccgctggc gtaacatgtg ggactggggc atcttcattg gtagcttcgt tccgccgctg
     3901 gtaattggtg ttgcgttcgg taacctgttg cagggcgtac cgttcaacgt tgatgaatat
     3961 ctgcgtctgt actacaccgg taacttcttc cagttgctta acccgttcgg cctgctggca
     4021 ggcgtggtga gcgtagggat gatcattact cagggcgcaa cctatctgca aatgcgtacc
     4081 gtgggcgaac tgcacctgcg tacccgtgca acggctcagg tggctgcgct ggtgacactg
     4141 gtctgtttcg cactggctgg cgtatgggtg atgtacggta tcgatggtta tgtcgtgaaa
     4201 tcgacaatgg accattacgc agcctctaac ccactgaata aagaagtggt tcgtgaagct
     4261 ggcgcatggc tggttaactt caacaacacg ccaattctgt gggctattcc ggcactgggt
     4321 gtggttctgc cgctgctgac catcctgact gcacgtatgg ataaagccgc gtgggcgttt
     4381 gtgttctcct ccctgacgct ggcctgcatc atcctgacag ccggtatcgc aatgttcccg
     4441 tttgtgatgc cgtccagcac catgatgaac gcaagtctga caatgtggga tgcaacttcc
     4501 agccagttga cgcttaacgt catgacctgg gttgcggtgg ttctggtacc gatcattctg
     4561 ctctacaccg cctggtgtta ctggaaaatg ttcggtcgta tcaccaaaga agatattgaa
     4621 cgtaacaccc actctctgta ctaagtaagg agctaaaaat gtggtatttc gcatggattc
     4681 tgggaacgct tcttgcctgt tcgtttgggg taatcaccgc gctggcgctt gaacacgtcg
     4741 aatcaggcaa agccggtcaa gaagacatct gatgagtaag attatcgcga ccttgtatgc
     4801 ggtaatggac aagcgccccc tgcgggcgct ttccttcgtg atggcgcttc tgttagcagg
     4861 atgtatgttt tgggacccat ctcgttttgc cgcgaagacc agtgaactgg aaatctggca
     4921 tggtttattg ctgatgtggg ccgtctgtgc tggtgtgatt cacggcgtgg gctttcgtcc
     4981 gcagaaggtt ctttggcaag ggattttttg cccattgctt gccgatattg ttctcattat
     5041 cgggctgatt ttcttcttct tttaaatcag aattctcttt aaaaaattat gggccgctcc
     5101 aggcccataa atttttacgc tcccttaact tgccctcatt cccaaacctc aatcgcgcgc
     5161 gtatagtagc agcgtttaaa agctctaact tttgttgcat taccgggatg taaagtgaat
     5221 acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg
     5281 gtgtaccacg ccagttacgt cgctttttat gaaagagcac gcacagagat gctgcgtcat
     5341 catcacttca gtcagcaggc gctgatggct gaacgcattg cctttgtggt acgtaaaatg
     5401 acggtggaat attacgcacc tgcgcggctc gacgatatgc tcgaaataca gactgaaata
     5461 acatcaatgc gtggcacctc tttggttttc acgcaacgta ttgtcaacgc cgagaatact
     5521 ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt
     5581 gcgcttccca agtctattgt cgcggagttt aagcagtgac tgacatgaat atccttgatt
     5641 tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt gattttgatt ggtttttcaa
     5701 tcgcatcctg ggccattatt atccagcgga cccgtattct taacgcagcg gcgcgcgaag
     5761 ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact ctctcgcctc tatcaagaga
     5821 gtcaggggaa acgggataat ctgactggtt cggaacaaat attttacagc gggttcaaag
     5881 agtttgtgcg cctgcatcgt gccaatagcc atgcgccgga agccgtagtg gaaggggcgt
     5941 cgcgtgctat gcgtatctcc atgaaccgtg aacttgaaaa tctggaaacg cacattccgt
     6001 tcctcggtac ggttggctcc atcagcccgt atattggtct gtttggtacg gtctggggga
     6061 tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa atggttgcgc
     6121 ccggtatcgc agaagcgttg attgcgactg caattggtct gtttgccgct atcccggcag
     6181 ttatggccta caaccgcctc aaccagcgcg taaacaaact ggaactgaat tacgacaact
     6241 ttatggaaga gtttaccgcg attctgcacc gccaggcgtt taccgttagc gagagcaata
     6301 aggggtaagt catggccaga gcgcgtggac gaggtcgtcg cgaacttaag tccgaaatca
     6361 acattgtacc gttgctggac gtactgctgg tgctgttgct gatcttcatg gcgacagcgc
     6421 ccatcatcac ccagagcgtg gaggtcgatc tgccagacgc tactgaatca caggcggtga
     6481 gcagtaacga taatccgcca gtgattgttg aagtgtctgg tattggtcag tacaccgtgg
     6541 tggttgagaa agatcgcctg gagcgtttac caccagagca ggtggtggcg gaagtgtcca
     6601 gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt
     6661 acgatgaaat aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt
     6721 taatgacgca gcctatctaa acatctgcgt ttcccttgct tgaaagagag cgggtaacag
     6781 gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg aacaaaacga caagctcaag
     6841 cgggcgataa ttatttcagc agtgctgcat gtcatcttat ttgcggcgct gatctggagt
     6901 tcgttcgatg agaatataga agcttcagcc ggaggcggcg gtggttcgtc catcgacgct
     6961 gtcatggttg attcaggtgc ggtagttgag cagtacaaac gcatgcaaag ccaggaatca
     7021 agcgcgaagc gttctgatga acagcgcaag atgaaggaac agcaggctgc tgaagaactg
     7081 cgtgagaaac aagcggctga acaggaacgc ctgaagcaac ttgagaaaga gcggttagcg
     7141 gctcaggagc agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag
     7201 caagctgaag aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcagat
     7261 gctaaagctg cggaagaagc agcgaagaaa gcggctgcag acgcaaagaa aaaagcagaa
     7321 gcagaagccg ccaaagccgc agcgcagaaa aaagccgagg cagccgctgc ggcactgaag
     7381 aagaaagcgg aagcggcaga agcagctgca gctgaagcaa gaaagaaagc ggctgctgaa
     7441 aaggctgcag ctgataagaa agcggcagcc gacaaaaaag cagcagaaaa agcggctgct
     7501 gaaaaggcag cagctgataa gaaagcagcg gcagaaaaag ccgccgcagc ggcagaaaaa
     7561 gccgccgcag aggcagatga tattttcggt gagctaagct ctggtaagaa tgcaccgaaa
     7621 acggggggag gggcgaaagg gaacaatgct tcgcctgccg ggagtggtaa tactaaaaac
     7681 aatggcgcat caggggccga tatcaataac tatgccgggc agattaaatc tgctatcgaa
     7741 agtaagttct atgacgcatc gtcctatgca ggcaaaacct gtacgctgcg cataaaactg
     7801 gcgcccgatg gtatgttact ggatatcaaa cctgaaggtg gagatcccgc actttgtcag
     7861 gctgcgttgg cagcagctaa acttgcgaag atcccgaaac caccaagcca ggcagtatat
     7921 gaagtgttca aaaacgcgcc attggacttc aaaccgtaat cgcgatgttg actgttcgga
     7981 cggtcaacat caggcaccgg ttgccacggg gttctggtag ttttgtgtat tttagtttgt
     8041 taacattctg ctaaattatc gtgggccatc ggtccagata agggagatat gatgaagcag
     8101 gcattacgag tagcatttgg ttttctcata ctgtgggcat cagttctgca tgctgaagtc
     8161 cgcattgtga tcgacagcgg tgtagattcc ggtcgtccta ttggtgttgt tcctttccag
     8221 tgggcggggc ctggtgcggc acctgaagat attggcggca tcgttgctgc tgacttgcgt
     8281 aacagcggta aatttaatcc gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg
     8341 caggaagtac aaccatctgc atggtccgca ctgggcattg acgctgtagt tgtcggtcag
     8401 gtcactccga atccggatgg ttcttacaat gttgcttatc aacttgttga cactggcggc
     8461 gcaccgggta ctgtacttgc tcagaactcg tacaaagtga acaagcagtg gctgcgttat
     8521 gctggtcata ccgccagtga tgaagtgttt gaaaaactga ccggcattaa aggtgcgttc
     8581 cgtacccgta ttgcctacgt tgttcagacc aacggcggtc agttcccgta tgaactgcgc
     8641 gtatctgact atgacggtta caaccagttt gtcgttcacc gttcaccgca gccgctgatg
     8701 tctccggcgt ggtcaccaga cggttctaaa ctggcttatg tgaccttcga aagcggtcgt
     8761 tccgcgctgg ttattcagac gctggcaaat ggcgctgtac gtcaggtggc ttcattcccg
     8821 cgtcacaacg gtgcacctgc attctcgcca gacggcagca aactggcatt cgccttgtcg
     8881 aaaaccggta gtctgaacct gtacgtaatg gatttggctt ctggtcaggt ccgccaggtg
     8941 actgatggtc gcagtaacaa taccgaaccg acctggttcc cggatagcca gaacctggca
     9001 tttacttctg accaggccgg tcgtccgcag gtttataaag tgaatatcaa cggcggtgcg
     9061 ccacaacgta ttacctggga aggttcgcag aaccaggatg cggatgtcag cagcgacggt
     9121 aaatttatgg taatggtcag ctccaatggt gggcagcagc acattgccaa acaagatctg
     9181 gcaacgggag gcgtacaagt tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca
     9241 cctaatggca ctatggtaat ctacagctct tctcagggga tgggatccgt gctgaatttg
     9301 gtttctacag atgggcgttt caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc
     9361 cctgcctggt cgccgtatct gtgataataa ttaattgaat agtaaaggaa tcattgaaat
     9421 gcaactgaac aaagtgctga aagggctgat gattgctctg cctgttatgg caattgcggc
     9481 atgttcttcc aacaagaacg ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac
     9541 tggtatggat gcgaacggcg gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca
     9601 aatgcaacag ctgcagcaga acaacatcgt ttacttcgat ctggacaagt acgatatccg
     9661 ttctgacttc gctcaaatgc tggatgcaca tgcaaacttc ctgcgtagca acccgtctta
     9721 caaagtcacc gtagaaggtc acgcggacga acgtggtact ccggaataca acatctccct
     9781 gggtgaacgt cgtgcgaacg ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga
     9841 ccagatctcc atcgtttctt acggtaaaga aaaacctgca gtactgggtc atgacgaagc
     9901 ggcatactcc aaaaaccgtc gtgcggtact ggtttactaa gagaattgca tgagcagtaa
     9961 cttcagacat caactattga gtctgtcgtt actggttggt atagcggccc cctgggccgc
    10021 ttttgctcag gcaccaatca gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcactca
    10081 acttgagcgt atttctaatg ctcacagcca gcttttaacc caactccagc aacaactttc
    10141 tgataatcaa tccgatattg attccctgcg tggtcagatt caggaaaatc agtatcaact
    10201 gaatcaggtc gtggagcggc aaaagcagat cctgttgcag atcgacagcc tcagcagcgg
    10261 tggtgcagcg gcgcaatcaa ccagcagcga tcaaagcggt gcggcggcat caacgacgcc
    10321 gacagctgat gctggtactg cgaatgctgg cgcgccggtg aaaagcggcg atgcaaacac
    10381 ggattacaat gcagctattg cgctggtgca ggataaatcc cgccaggatg acgcaatggt
    10441 ggcatttcag aatttcatca aaaattaccc tgattcaact tacctgccaa acgccaatta
    10501 ttggctgggt cagttaaact acaacaaggg taaaaaagat gatgcggcgt actattttgc
    10561 ttcggtagtg aaaaactatc cgaagtcacc aaaggctgct gatgcgatgt ttaaagtcgg
    10621 cgtcatcatg caggacaaag gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat
    10681 cagtaaatac cctggtactg atggcgctaa acaggcacaa aaacgtctga acgcgatgta
    10741 atgcataaca cacgaccaga agttgcatta tttctggtcg tgtcgtgcga atcataagca
    10801 gttgagtgat ctgcatcgaa atttttgttg cgctcaagtc tgaaatcagt aatatatgcc
    10861 gccgttgcca cgggatatca aacaaaccga aagcaacgaa aaagtgggtc gttagctcag
    10921 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
    10981 actaacatag ttagttgtag tatccagcgt agtattgggt gattagctca gctgggagag
    11041 cacctcc
//
LOCUS       DAJTMK010000132        10951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTMK010000132 DAJTMK010000000
VERSION     DAJTMK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.134"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(163..495)
                     /gene="emrE"
                     /locus_tag="OO135_003036"
     CDS             complement(163..495)
                     /gene="emrE"
                     /locus_tag="OO135_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5398798.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYYASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(829..1143)
                     /gene="fliE"
                     /locus_tag="OO135_003037"
     CDS             complement(829..1143)
                     /gene="fliE"
                     /locus_tag="OO135_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5398799.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1358..2527
                     /gene="fliF"
                     /locus_tag="OO135_003038"
     CDS             1358..2527
                     /gene="fliF"
                     /locus_tag="OO135_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR5398800.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEAILSPIVGNGNIHAQVTA
                     QLDFASKEQTEEQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNA
                     PISTPPANQNNRQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAV
                     VVNYKTLPDGKPLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGEL
                     PFWQQQAFIDQLLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVE
                     DAVEVRLSKDEQLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2520..3515
                     /gene="fliG"
                     /locus_tag="OO135_003039"
     CDS             2520..3515
                     /gene="fliG"
                     /locus_tag="OO135_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5398801.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAIANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3508..4194
                     /gene="fliH"
                     /locus_tag="OO135_003040"
     CDS             3508..4194
                     /gene="fliH"
                     /locus_tag="OO135_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5398802.1"
                     /translation="MSDNLPWKTWTPDDLAPPEAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATFSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4194..5481
                     /gene="fliI"
                     /locus_tag="OO135_003041"
                     /pseudo
     CDS             4194..5481
                     /gene="fliI"
                     /locus_tag="OO135_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            5417..6571
                     /gene="fliK"
                     /locus_tag="OO135_003042"
     CDS             5417..6571
                     /gene="fliK"
                     /locus_tag="OO135_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5398803.1"
                     /translation="MPKAAIRCSTIRLAPLITADVDTTTLPGGKASDAAQDFLALLSE
                     ALAGETTTDKAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQS
                     TSTPLTTAQTMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVP
                     STVLPAEKPTLFTKLTSAQLTTAQPDDSPGTPAQPLTPLVAEAQSKAEVISTPSPVTA
                     AASPLITPHQTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQD
                     LGEVQISLKVDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESF
                     SGQQQAASQQQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            6676..7140
                     /gene="fliL"
                     /locus_tag="OO135_003043"
     CDS             6676..7140
                     /gene="fliL"
                     /locus_tag="OO135_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5398804.1"
                     /translation="MTDYSISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            7145..8149
                     /gene="fliM"
                     /locus_tag="OO135_003044"
     CDS             7145..8149
                     /gene="fliM"
                     /locus_tag="OO135_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5398805.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKSLRGAGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            8146..8559
                     /gene="fliN"
                     /locus_tag="OO135_003045"
     CDS             8146..8559
                     /gene="fliN"
                     /locus_tag="OO135_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5398806.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            8562..8927
                     /gene="fliO"
                     /locus_tag="OO135_003046"
     CDS             8562..8927
                     /gene="fliO"
                     /locus_tag="OO135_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5398807.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            8927..9589
                     /locus_tag="OO135_003047"
     CDS             8927..9589
                     /locus_tag="OO135_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5398808.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDNIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELMALGMMMVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF
                     YS"
     gene            9599..9868
                     /gene="fliQ"
                     /locus_tag="OO135_003048"
     CDS             9599..9868
                     /gene="fliQ"
                     /locus_tag="OO135_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5398809.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            9877..10658
                     /gene="fliR"
                     /locus_tag="OO135_003049"
                     /pseudo
     CDS             9877..10658
                     /gene="fliR"
                     /locus_tag="OO135_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
BASE COUNT         2632 a         2887 c         2929 g         2503 t
ORIGIN      
        1 ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt atgcaaaaag
       61 gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat tagccataat
      121 atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg gtgcgcttcg
      181 tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta tgatggctgg
      241 caagtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa cgataccgac
      301 tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct gagctaataa
      361 ccaaaatgat gcataatagc aaataattgt gccaacagag ggccataacc gtgtaaaccc
      421 ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc caccaagata
      481 aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg tactacagtt
      541 tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc tggcgtggca
      601 agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa aagagttcac
      661 tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc gtggatggca
      721 gaagactttc aggatcaggt atccatttta aaccagaaat agggtgagtt tgtcccatcc
      781 atgccccacg cgggcagatg ggatgtaatt accaacaggt tacaaaacct acacctgcat
      841 ggacatcact tcctgatacg ccgccaccag cttattacgc acctgaatcc ccatttgcat
      901 agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc cgggttcacc
      961 gagggtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta tgcgatcgag
     1021 cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc ggcagtgaat cctgcgcacg
     1081 cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga
     1141 cattttcgtc tcctggataa tttctggtag caaagcctac cagtaagtca ataagacaaa
     1201 ggcgctaaat agcaacaaaa aaacgggttt attggcggat agaaaaaaac gaaagcacaa
     1261 ataatggcag cgtcaatttt tcgagtttgc tgacccggga gtgagtcttg ttccactttg
     1321 ccaataacgc cgtccataat cagccacgag gtgcgcgatg aatgcgactg cagcccagac
     1381 aaaatctctt gagtggctta atcgcctgcg tgcgaatccg aaaattccat tgattgttgc
     1441 cggttccgcg gcagtggcgg tcatggtcgc actgatcctg tgggcgaaag cccccgacta
     1501 ccgcacatta ttcagcaatc tttccgatca ggatggtggc gcaattgtca gccaactgac
     1561 gcaaatgaat attccttacc gcttcagcga agccagcggc gctattgaag cgatcctgtc
     1621 gccaattgtt ggtaacggta atattcacgc ccaggtcacg gcgcaactgg acttcgccag
     1681 taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg cggcgcttcg
     1741 ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg gcgtaccggg
     1801 ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc ctccggcaaa
     1861 tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc gtagcacaca
     1921 gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt
     1981 gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct tgccagacgg
     2041 taaaccattg cctctcagca acgaacagat gaagcaaatt gaagatctga cccgcgaggc
     2101 gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc cgttcaatag
     2161 cagtgacgaa agcggcggag aactgccgtt ctggcaacag caagcgttta tcgatcagtt
     2221 gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt ggcggaaagc
     2281 ggtacgcccg cagttaacaa gtcgcgccga ggcgatgaaa gctgtacagc aacaggcgca
     2341 ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg aacaactcca
     2401 acaacgacgc gctaaccaac gtctgggggc agaagtcatg agccagcgta tccgtgaaat
     2461 gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa ataacgatca
     2521 tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt ggcgaagacc
     2581 gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa
     2641 ttgcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg gagtttgagc
     2701 aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg cgttcggtat
     2761 tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt ctcgaaactc
     2821 gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagt gccgccgatc
     2881 tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg aagcgcgccc
     2941 aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta
     3001 tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa gtactgaatg
     3061 gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg
     3121 aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc gtgcgcgaat
     3181 tcgacggcga gctggcgcaa aaaattatcg acgagatgtt cctgttcgaa aatctggtgg
     3241 atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa tcgctgttga
     3301 tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc
     3361 gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg
     3421 tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa actggcgaga
     3481 tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa aacctggacg
     3541 ccggacgatc tcgcgccacc agaggcagag tttgtgccca tggtcgagcc ggaagaaacc
     3601 atcattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca aatgcaggcc
     3661 catgagcaag gttatcaggc ggggattgcc gaaggtcgcc agcaaggtca tgagcagggc
     3721 tatcaggaag gactggccca ggggctggag caaggtctgg cagaggcgaa gtctcaacaa
     3781 gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg
     3841 cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcagc acgtcaggtc
     3901 atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca acagttgttg
     3961 cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga tgatctgcaa
     4021 cgtgtggatg atatgctcgg cgctaccttc agtttgcatg gctggcgctt gcggggcgat
     4081 cccaccctcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct cgacgccagt
     4141 gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt gtaataacca
     4201 cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg gcgcagttgc
     4261 ctgcggtacg tcgctacggg cgattaaccc gcgctaccgg gctggtgctg gaagccaccg
     4321 gattacaatt gccgctcggc gcaacctgtg tgattgagcg ccagaacggc actgaaacgc
     4381 acgaagtaga aagtgaagtc gttggcttta acggtcaacg gctgttttta atgccgctgg
     4441 aggaagtcga aggtgtcctg cccggcgcgc gtgtttatgc caaaaacatt tcggcagaag
     4501 ggctgcaaag cggcaaacag ttaccgctcg gtccggcgtt attaggtcgt gttctggacg
     4561 gcagcggtaa accgctcgat ggcctccccc gatacgacgg aaaccggtgc gctgattacc
     4621 ccgccattta acccgttgca acgtacaccg attgaacatg tgctggacac cggcgtgcgc
     4681 ccaatcaatg ccctgcttac cgttgggcgt gggcagcgta tggggctgtt tgccgggtcc
     4741 ggcgttggta aaagtgtgct gctggggatg atggcacgtt acacccgcgc cgatgtcatt
     4801 gtcgtgggtt tgattggtga acgcggacgc gaagtaaaag attttattga gaatatcctc
     4861 ggtgccgaag ggcgtgcacg ctccgtggtg attgccgctc cggcggatgt ttctcctctc
     4921 ctgcgaatgc agggtgccgc ctatgccacg cgcattgccg aagattttcg cgatcgtggt
     4981 cagcatgtgt tgctgattat ggactccctc acccgctacg cgatggccca gcgtgaaatt
     5041 gcgctggcga ttggcgaacc acccgccact aaaggttatc ccccgtcggt gtttgccaaa
     5101 ttaccggcac tggtcgagcg tgccggaaat ggcattagcg gcggcggatc gattaccgcg
     5161 ttttataccg tgctcactga aggcgatgac cagcaggacc ccattgccga ctctgcgcgg
     5221 gcaatcctcg acggtcacat tgtgctgtct cgccgactgg cggaagccgg gcactatccg
     5281 gctatcgata ttgaagcgtc gatcagccgc gcaatgacgg cgttgatcag tgagcaacat
     5341 tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg
     5401 gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc tcgacaattc gcttagcgcc
     5461 cttaattacc gccgacgttg acaccaccac attgcctggc ggcaaagcca gcgatgctgc
     5521 acaagatttt ctcgcgttgt tgagcgaagc attagcaggc gagacaacta ccgacaaagc
     5581 ggccccccag ttgctggtgg caacagataa gcccacgaca aaaggcgagc cgctgatcag
     5641 cgatattctt gccgacgcgc aacaagcgga tttactgatc cccgtggatg aaacactgcc
     5701 tgtcatcaac gacgaacaat ccacatcaac accgttaacc accgctcaga cgatgacgtt
     5761 ggctgcggtg gctgaccaaa atacgacaaa agacgaaaaa gcggatgatc tgaatgaaga
     5821 cgtcaccgca agcctgagcg ccctttttgc gatgttgccg ggttttgaca atacgcccaa
     5881 agtgactgat gtgccgtcaa ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact
     5941 gacttctgcg caactcacaa cagcacagcc tgatgattcc cccggcacgc cagctcagcc
     6001 attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca caccgtcacc
     6061 ggtgaccgct gccgccagcc cgctaattac tccacaccag acacagccac tgcccaccgt
     6121 cgccgcgcct gttttgagtg caccgctggg ttctcacgaa tggcaacaat cattaagcca
     6181 gcatatttcg ctgttcaccc gccaggggca acaaagtgca gagttgcgtc tgcacccgca
     6241 ggatttaggt gaagtgcaaa tctccctcaa agtggatgat aaccaggcgc aaatccagat
     6301 ggtttcaccg catcagcatg tacgcgccgc gctggaagca gcgcttccgg tactgcgtac
     6361 gcagctggcc gaaagtggca ttcagttagg gcaaagcaac atcagtggcg aaagctttag
     6421 tggtcagcag caggccgctt cccaacaaca gcaaagccaa cgcacagtaa accatgaacc
     6481 tctggcgggg gaagacgacg atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac
     6541 aggcaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta atccgcgtct
     6601 tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag tagcgacaca
     6661 ggaagaccgc aacacatgac tgattactcg ataagcaaga aaagcaagcg atcgctttgg
     6721 atcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta cagctactgg
     6781 cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac gcgtcgtacc ctccccggtc
     6841 ttctacgcgc tggatacctt cacggtcaat ttgggcgatg cggatcgcgt gctttatatc
     6901 ggcataaccc tgcgcctgaa agatgaagct acccgctcgc ggctgagtga gtatttgccg
     6961 gaagtccgta gccgtttgct gttactgttt tcgcgtcagg atgctgccgt actggcgaca
     7021 gaagaaggca agaaaaacct gattgccgag attaaaacca cgctttccac cccgcttgtt
     7081 gccgggcaac cgaaacagga tgtcaccgac gtgctgtata ccgcttttat tctgcgataa
     7141 cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga atggtgacag
     7201 cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa agtgacattc gtccgtacga
     7261 tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga
     7321 gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgtcgta gcccggatat
     7381 aaccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc
     7441 gaccaacctg aaccttatcc atctgaaatc gctgcgcggc gcagggctgg tggtgttttc
     7501 accgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcgatggac gcttcccgac
     7561 caaagtggaa ggtcgcgagt ttacccatac cgaacagcgc gtcatcaacc gcatgttaaa
     7621 gctggcgctt gaaggctata gcgacgcctg gaaggcgatt aatccgctgg aagttgagta
     7681 cgtgcgttcg gaaatgcagg tgaaatttac caatatcacc acctcgccga acgacattgt
     7741 ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta atatctgtct
     7801 gccattcagc atgatcgagc cgctacggga attgttggtt aacccgccgc tggaaaactc
     7861 gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc attcacagct
     7921 ggagctggtc gccaactttg ccgatatctc gctacgcctg tcgcagattt taaaactgaa
     7981 acccggcgac gtcctgccga tagaaaaacc tgatcgcatc atcgcccatg ttgacggcgt
     8041 cccggtgctg accagtcagt acggcactct caacggtcag tatgcgttac ggatagaaca
     8101 tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag tgacatgaat
     8161 aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc gttgagcgaa
     8221 caaaaatcaa ccagcagcaa aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt
     8281 gatgtcagcg gaacgttgca ggatatcgac ctgataatgg atattccggt caagctgacc
     8341 gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac gcaagggtcc
     8401 gtcgtggcgc tggacggtct ggcgggcgaa ccactggata ttctgatcaa cggttattta
     8461 atcgcccagg gcgaagtggt ggtcgttgcc gataaatatg gcgtgcggat caccgatatc
     8521 attactccgt ctgagcgaat gcgtcgcctg agccgttagt gatgaataac cacgctactg
     8581 tgcaatcttc cacgccggtt tctgctgcgc cactgctgca ggtcagcggc gcactcatcg
     8641 ccattattgc cctgatcctc gctgctgcct ggcaggtaaa acggttggga tttgccccga
     8701 aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac
     8761 gggttgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc gcagggcaaa
     8821 tcaatctgct gcataaactt cccccttcag caccaacaga agagataccg cagaccgatt
     8881 ttcagtcggt catgaaaaat ttgcttaagc gtagtgggag atcctgatgc gtcgtttgtt
     8941 gtctgtcgca cctgtccttc tctggctggt tacgcccctc gccttcgcgc aactgccggg
     9001 tatcaccagc cagccgctgc ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct
     9061 ggtgttcatc acctcgttga cgtttattcc ggcaatttta ctgatgatga ccagtttcac
     9121 ccgcatcatc attgtttttg gtttattgcg taacgcgctg ggaacgccct ccgcgccacc
     9181 taaccaggta ttgctggggc tggcactatt tttgaccttt tttattatgt caccggtgat
     9241 cgacaacatt tatgtcgatg cgtaccagcc attcagcgaa gagaaaatat caatgcagga
     9301 ggcgctggaa aaaggggcgc agccgctacg tgagtttatg ctgcgtcaga cccgtgaggc
     9361 agatttaggg ttgtttgcca gactagcgaa taccggcccg ttgcagggac ctgaagccgt
     9421 gccgatgcgc attttgctcc cggcctacgt gaccagcgag ttgatggcat tggggatgat
     9481 gatggtcccc cccgccacca ttgctctgcc ctttaaactg atgctgtttg tactggtgga
     9541 tggctggcaa ttgctggtcg gttcgctggc gcagagcttt tacagctaga gaggcaaaat
     9601 gacacctgaa tcggtcatga tgatggggac tgaagcgatg aaagtcgcgc tggcactggc
     9661 tgccccgctg ttgctggtgg cgctggtcac gggccttatc atcagtattt tgcaggccgc
     9721 cacgcagatc aacgaaatga cgctgtcgtt tattccaaaa atcatcgccg tatttattgc
     9781 cattattatt gccggaccgt ggatgctcaa tctgttgctg gattacgtcc gcaccttgtt
     9841 cactaacctg ccgtatatca tcgggtaggc cgtacaatga tgcaggtgac aagcgatcaa
     9901 tggctttcct ggttaagcct gtacttctgg ccgttactgc gcgtgctggc gctcatttct
     9961 accgcaccaa ttctgagcga acgcagcgtg ccgaaacggg taaaactggg gctggcaatt
    10021 atgatcacgt tcgtcattgc cccatcatta cctgccaacg atgttcctgt tttttcgttc
    10081 tttgctctgt ggctggccgt gcagcagatc ctgatcggca ttgcgcttgg ttttaccatg
    10141 caatttgcct ttgccgctgt gcgaaccgct ggcgaaatta tcggtctgca aatggggctg
    10201 tcatttgcga cgtttgtcga tccggccagc catctcaata tgcccgtttt agcgcgtatc
    10261 atggatatgc tggcgttact gctgttcctg acatttaacg gtcatttatg gttgatttca
    10321 ctgctggtcg atacctttca caccctgcca attggtggcg aaccgttgaa cagcaatgcg
    10381 tttctggcac tcaccaaagc ggggagtttg attttcctta acgggctgat gctggcgtta
    10441 ccgctcatta ctctgctgct gaccctgaat ctggcattag gtttactgaa tcgtatggcc
    10501 ccgcaattat ccatttttgt tattggattt ccattaactc tgactgtcgg tatctcttta
    10561 atggcggcat taatgccgtt aattgcacct ttttgcgaac atttattcag tgagatgttt
    10621 agctggctga tattattagt gaattgccat taatataatt ccgtaacgtt tatcatgtta
    10681 tcctaaggat tatccgaaaa ataataccta cgaacatctt ccaggatact cctgcggcga
    10741 aatatttgtt ttaagcttac tcacatatcg caacatttac tttaagacaa ttccaggcaa
    10801 attatacaac actttacggg atagtaagtc cgcctgaaaa atcgcgagag tggcgcatta
    10861 ggtgacccat gttgttccgt tagtcatgat gaaatattca ggtaagggga attatcgtta
    10921 cgcattgagt gagggtatgc catgtcaacg a
//
LOCUS       DAJTMK010000133        10899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTMK010000133 DAJTMK010000000
VERSION     DAJTMK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10899
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.135"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(229..2088)
                     /gene="gss"
                     /locus_tag="OO135_003050"
     CDS             complement(229..2088)
                     /gene="gss"
                     /locus_tag="OO135_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5398810.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLN
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSCARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2293..3159
                     /gene="yghU"
                     /locus_tag="OO135_003051"
     CDS             2293..3159
                     /gene="yghU"
                     /locus_tag="OO135_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5398811.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFFHYAPVKTEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3282..3530)
                     /gene="hybG"
                     /locus_tag="OO135_003052"
     CDS             complement(3282..3530)
                     /gene="hybG"
                     /locus_tag="OO135_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5398812.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3543..3884)
                     /gene="hypA"
                     /locus_tag="OO135_003053"
     CDS             complement(3543..3884)
                     /gene="hypA"
                     /locus_tag="OO135_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5398813.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3877..4365)
                     /gene="hybE"
                     /locus_tag="OO135_003054"
     CDS             complement(3877..4365)
                     /gene="hybE"
                     /locus_tag="OO135_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5398814.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4358..4852)
                     /locus_tag="OO135_003055"
     CDS             complement(4358..4852)
                     /locus_tag="OO135_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5398815.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4852..6555)
                     /gene="hybC"
                     /locus_tag="OO135_003056"
     CDS             complement(4852..6555)
                     /gene="hybC"
                     /locus_tag="OO135_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5398816.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6552..7730)
                     /gene="hybB"
                     /locus_tag="OO135_003057"
     CDS             complement(6552..7730)
                     /gene="hybB"
                     /locus_tag="OO135_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5398817.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGDLIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLCVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLITIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7720..8706)
                     /gene="hybA"
                     /locus_tag="OO135_003058"
     CDS             complement(7720..8706)
                     /gene="hybA"
                     /locus_tag="OO135_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5398818.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVYYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8709..9827)
                     /gene="hybO"
                     /locus_tag="OO135_003059"
     CDS             complement(8709..9827)
                     /gene="hybO"
                     /locus_tag="OO135_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5398819.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10016..10303)
                     /locus_tag="OO135_003060"
     CDS             complement(10016..10303)
                     /locus_tag="OO135_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5398820.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10422..>10899)
                     /locus_tag="OO135_003061"
     CDS             complement(10422..>10899)
                     /locus_tag="OO135_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5398821.1"
                     /translation="GGYPGNDDKGRELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVG
                     ITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADVAKIEAPLLLHFAELDTRINEG
                     WPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
BASE COUNT         2659 a         2992 c         2690 g         2558 t
ORIGIN      
        1 gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt atttcttgtg tatcagacaa
       61 taagcattca ttcaaaacct ttagaaataa tcaaaacaca ggtattccac tggttaatat
      121 attcacatat gaaatgaata aatattagaa catataaata ttgaatattt tgattaatcc
      181 ccgtactgat tattcttcat aatcagtacg gggttgctac aacatgtatt actttttcac
      241 cacaattaac ggttcaatat cactctcttt tttgatgacc agtgattcat caccgcgcaa
      301 acacgtccca ccgtagttgc cgcccacggt gaaggtacat acctgaatgt atttaccgtc
      361 cactttcggc aaacaccaca gttgctgata gatgtttttc tgctcggcaa atttaccgct
      421 ggttttgtcc agcacctctt catgatggct gaccaggtcg atattgctac cacagcgacc
      481 ggcaatcggt ttcactgcgt aacctgtttt caccaattca tcattaacag tgaaatcggt
      541 atccagcagg taacgatggt gcgggaacag cgaccagagg atcggcagaa tcgctttgtt
      601 gccggggatc accgtccaca gcggctcaaa gactagcact tccgggcgca gcaatacgtc
      661 gataagacgc acttcgtttt gcggatgacc ggtacggatt ggcaccgcag caaactcacg
      721 gtcgctaact tcacgaatct gatcaaacgc ggtttcccac gcccaggttt tccacacgca
      781 gttaaccagt cgcccttccc catcaatcag ttgcccggca gcatcccagc ccagttcatc
      841 caatccacgc aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct ccataaactg
      901 cgcgtgatag ttttcctcga tatctttgtc ctgcatgata tggacaaacg gacgtgcaca
      961 actgtgtttc caggcaccag ccaattcgtt aatcagccct tccgccggat tgaagccgtt
     1021 gcctttatag ccctgctccg cccaacgttc gaggatcaag cccgcttcgg tatgacagga
     1081 ggcggagtcg gcgttgtact cgtaaacctt caggccacgc tcatccatgc agaaatccat
     1141 acgaccagtg atcatatggt gacggcgacg ctgccaggag agacgcaaac gtggccagag
     1201 gattttcggg atgtcgaaca gcgccagcag gttgtcatct ttcagtacct tgtcggttgc
     1261 gttaagatac atcaggtgca gctcgttggt ggctttaatt agctcctgct cggcactctc
     1321 ggtaatggtg tagtaatgat aaggatcctg attgatcacc tgaccgttgg cctgcacata
     1381 ggcgttttgc agcggatctt tttcatccag ccatttaccg tcaaactggc ctttgttttc
     1441 caggcgcgct ccgctgattt tcagcagctc gcctgcaatt tccggctgcg gtaagctgta
     1501 ttcagtatct tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt
     1561 cagggtatag cagccgtttt cgaccaccat ctccagctca cgcgtccact gttgcccttg
     1621 cggcaacggg gaatgaatca cgttctgttc cgcaatacgg actttgttgc catgcaattg
     1681 ggtaatgatg gcgacatggc cagtgtcttt aaattcaccg cctttatccc agataagaag
     1741 cgcacccgcg accggcgcac gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt
     1801 gtcattaacc acttctcgca ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc
     1861 agtaaagacc acaccgtaat tcagaaagag aaaacggcga gcaaattcaa cgcattgcca
     1921 cttgtggccc atatattcgt cgtcgatata gctacggaat acggcgtcat cttcgtattc
     1981 ctgcggatcg agagaactgt aatctgaaga gtagattgct accccacctg gggcgtagcc
     2041 caataatgtc ccgaacgggg catcctggct ggtcgttcct ttgctcatca ctttaccttt
     2101 aacaatacaa cctaagcagt tggcgtaatt gttgtgctct gattacctgc tcagcgacat
     2161 taaccggaca gaggtcagac taatcataca cctcatcgca gcggctggcg caaagggtta
     2221 aaaaattcac attctgtaca gcaagtgacc tgccacactg cttcaacact accactcaga
     2281 aggcaactca ctatgacaga caatacttat cagcccgcga aagtctggac gtgggataaa
     2341 tccgctggcg gcgcgttcgc caatatcaat cgcccggttt ctggtccgac gcatgaaaaa
     2401 acgctgcccg ttggcaaaca cccattgcaa ctttattcgc tgggaacgcc gaacggtcag
     2461 aaagtaacga ttatgcttga ggagctgctg gcgctgggcg ttactggtgc agagtacgac
     2521 gcctggctga ttcgtattgg cgatggcgat caattctcca gcggctttgt cgaagtgaac
     2581 ccaaactcga agatcccggc gctgcgcgat catacgcata atccgccgat ccgtgtgttt
     2641 gaatctggtt cgatcctgct ttatctggcg gagaaatttg gctacttcct gccgcaggat
     2701 ttggcaaagc gtactgaaac gatgaactgg ctgttctggt tacagggcgc ggcaccgttc
     2761 ctcggcggtg gttttggtca ctttttccat tacgcaccgg taaagactga gtacgccatc
     2821 aaccgcttta ccatggaagc caaacgtctg ctcgacgtgc tggataagca actggcgcag
     2881 cataagtttg ttgcgggcga tgagtacacc attgcggata tggcgatttg gccgtggttt
     2941 ggcaacgtgg tgttaggtgg tgtgtatgat gccgctgagt ttcttgatgc gggcagttat
     3001 aagcatgtac aacgctgggc gaaagaagta ggcgaacgtc cggcggtgaa acgtgggcgt
     3061 attgttaacc gcaccaacgg accgctgaat gagcagttgc atgagcgcca tgacgccagt
     3121 gatttcgaga cgaatacgga agataagcgt caggggtaag ggttggtgtt cgtcgcagca
     3181 agccatccag gccggataag gcgttcacgc cgcatccggc aatcgtgcac aatgcctgat
     3241 gcgacgctgc cgcgtcttat caggcctacg aaagctaatc atcacgcgct ggtaatgtcg
     3301 taatccattt ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc gatgatgctc
     3361 atggcaaatc cgacgtgtac cagcacccac tggcccagta gatcggcagg gttaccttca
     3421 caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg cgcaagctgg
     3481 tgaatatctt caccgacagc cagcacctgg cctggaacgc caatacacat tattaactcc
     3541 ggttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac acgcaaccgc
     3601 tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac ctggctgcaa
     3661 tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc ttgcgccacc
     3721 gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac gcaggagagc
     3781 gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc cgcctgccgt
     3841 tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc cactcctgcg
     3901 accaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattcgtga ctggcagcga
     3961 aaggatcatt cgtgcgcagt catccgtcag gcgttggccc tcttcaatcg acatactgtg
     4021 cgaaagcggt gacatcagcg aacaggagag gtattgcgaa acaccgtcca gttcaccaac
     4081 ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca ctttgcgcag
     4141 cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg gggtgatcac
     4201 acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg gcattgaagg
     4261 atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg ctgcctgtac
     4321 ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc gcctcccgtg
     4381 ggatggcttc cacgcctgat tcgcgcagcg cagccagaac ctgctcaagc gcaggttcaa
     4441 tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc gggatcacgc
     4501 cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac aatacgtcgg
     4561 ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact tcttcatccc
     4621 gcaggatcat catcgttccc ggcgtgttct ttttcgacac aatggcatcc gcaataatca
     4681 gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg ccatcgagga
     4741 tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc cgcacaccga
     4801 tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc attacagaac
     4861 cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac aggccatgca
     4921 cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat cagcaaccgg
     4981 tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac gcggaccaga
     5041 gttccaggtt gatggaacaa ctgcctggta gttactgata ataccgtctt taataaccat
     5101 ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga actcacccgt
     5161 tgccggaatg ttcggtttca caaaggtggt gtgatcgcct ttgccgatat tggtgatcag
     5221 tgcactgtat tgattttgca ggatatcctg caattcgcag cagtgaacgg tacgaccaat
     5281 aatacggccc agcgtggagt gcagctgcgc cacttccagc gtgttgccag tcagtttctg
     5341 ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg ccgccagttt
     5401 caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag tcggtgattt
     5461 cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg tggtaccttc
     5521 ccacggtgcc tgcggcgctt cgtctttata ccaggagtgc ttcgcgcttt cctgaatgcc
     5581 tttgatcagg tattcatctg aatgagaagt gatcggacga tacgaggaca gatccgcatt
     5641 ctcaatgtag ccgcccggga acaggaagct gccgttttta ctgtcggtcg ggaattccgg
     5701 cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt agaacgcggc
     5761 aataactgcg gtatcaacct tataaacctg ttcaacaaag tcgctcagct tgtcgatgaa
     5821 agacttgatg tacatcaggc gctcaaggtt cagcacgccc aaaccgtcga ggttgatcgg
     5881 gttcgcgaca ccacctaccg ccaggttctg aatgtgcggc gttttaccgc ccagcagcgc
     5941 cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg ctaccgcaat
     6001 caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat tggcgaaaat
     6061 acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag tgaactcttc
     6121 cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt tggttgggtc
     6181 agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gaaagctgat agaaatgaac
     6241 gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt gcgcgttaac
     6301 cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg cgtgagtggt
     6361 agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat cgcggttttt
     6421 cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg aaacgacgcc
     6481 attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg gatcaatagt
     6541 aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg ttttaaagga
     6601 ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat agccacaaaa
     6661 ccaatagtaa tcaacagttc ttcccaggtc gggaagtagg cataaccgcc gcccgggttg
     6721 aatgccacca gcgaataggt cagacgccag gttgcacaac ctaacagtgc gctcagtgct
     6781 gacaggaaca gcatgcggga atcattacgc agcttcgcca cacacagaac gaccagcggg
     6841 aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc ggcaaacgct
     6901 aacgacagct tgtcgcgata gataagatcg ccaaagcgca gcacgatgaa aatcgccagc
     6961 aacacactga tggtgttggt cagcttgacg aacagactct tttcatccgg accgttgcca
     7021 cgcagacccg cctgcaccag cgaaccttca aagatgacaa tcgagaagcc catgatgaac
     7081 gccgtcagca gcgagaacag cggcaacatt tcatagctct gccacaacgg atgcaccttg
     7141 tagcccgccg agatcatcag tgaccccatt gaagactggt gcatggtcgg caacagcgcg
     7201 ccaagtgcaa tgatgaagaa catcaccttg ttcagacgct tgagagaaac cttccagccc
     7261 agacgttcaa acagtgccgg agcaaactcc agtgccatca cgccgatata gatggtcata
     7321 cagaccgccg tctcgaacag taccgagttc acgttgaagt gccccggaat gtagaagtac
     7381 ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca agccacccag tgagtaacca
     7441 aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg gttaaagacg
     7501 tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtaccaat cagcaggtca
     7561 aacgcgatcc acacgcccca cgggaagccg ccgttcaggt cagagacaga gcccagaccg
     7621 aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc aaaaatcatg
     7681 accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat gatcgtctcc
     7741 tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc ccgccagcac
     7801 agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg aacgcgcacc
     7861 ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac ccgtcagtac
     7921 cagaacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat atttcggcac
     7981 cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt attcgctgcc
     8041 aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg taccgaaaat
     8101 caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat cgagacgttc
     8161 cacacctttc tggttgcaca gctcgcactt atgcagcgca ccaaacgggt tgttgtagtc
     8221 gtacttcggc acgttgtacg gacaggcgac catgcagtaa cggcaaccgg tgcacacgtc
     8281 tttgtcgtaa tagacaatgc cggttttcgg atcttttttc agtgcagaga ccgggcacac
     8341 agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg cgtagccgtt
     8401 ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga tgttattggt
     8461 atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt tacgttcagg
     8521 gaagttgata tcctgacact tggtgacgca agcttggcag cctacgcaca aggtcgagtc
     8581 gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg catgactgac
     8641 agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa aattacgtct
     8701 gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg acccagttca
     8761 cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag caaaccaata
     8821 gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg tgaacgcgga
     8881 gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat accttcttcg
     8941 ttacagccat agcatgggtg accaatcgct accggccaca cgccgccaac atcgcagaat
     9001 tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg gtacaggcac
     9061 cagccttcac ggtggccttc atcaccgaac tctttggcaa aacgaccagc atcgaagtgc
     9121 gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg acgatttttg
     9181 tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc gaggaagttg
     9241 tgcgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag aacttcttgc
     9301 aggctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca cgcagagcag
     9361 gaaccgatag cgataatcgc tgctgcgcct tctgccgctt tgcggatgtg atccacaatc
     9421 ggctcaccag caaccatgca ataaataccg ttatctttta atgggatgga accatccaca
     9481 accaacacat actgcccttt gtacttctca agtgcgttat gtttgttctc ttcgacctga
     9541 tgaccgaagg cggcggaaag cacttcgtga tactccagag agatagtctc cagcacgagg
     9601 ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt gcactcctgc
     9661 gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc catctctgca
     9721 gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt cataaaatca
     9781 cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta tagttattcc
     9841 gttgcgaaga cctggcatat attttgcctc aatcgcaaaa tcaataatgc gatcgatgcg
     9901 ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg ggcgctaaaa
     9961 cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg tttgatcaat
    10021 tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta ttctcacgaa
    10081 agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata cccgcttccc
    10141 aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag cctaatacaa
    10201 aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg gcatgcgttg
    10261 cttttaatcc cgccattaag cctttgccaa aatggttatt catcgttcac ctcattcgct
    10321 atatattatc atatatagcg atattttagg caaagaacga cttttgcgca cactattttt
    10381 tcatgcttgt ttaagacgta atcccgtaag agataaaccc atcaggagag atatttatcg
    10441 aaccatttca gcgtcctttg ccaggcaaga tcggcggcag atttgtcata acgaggcgtg
    10501 gaatcattat ggaatccgtg attaaccccc ggatagatat acgcctcata gaccttatta
    10561 ttggctttca acgccgcctc ataagcgggc cagccctcgt tgattcgggt gtccagttcc
    10621 gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg tgcctgacga
    10681 ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc gttcgatacg
    10741 ccaccgccat agcaaaatcc ggtaataccc actttgcctg tcgcttgcgg atagcgttgc
    10801 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10861 agctcacgac ctttatcatc atttcccgga taacctcca
//
LOCUS       DAJTMK010000134        10811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTMK010000134 DAJTMK010000000
VERSION     DAJTMK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.136"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            122..988
                     /gene="folD"
                     /locus_tag="OO135_003062"
     CDS             122..988
                     /gene="folD"
                     /locus_tag="OO135_003062"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5398822.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            990..1202
                     /gene="ybcJ"
                     /locus_tag="OO135_003063"
     CDS             990..1202
                     /gene="ybcJ"
                     /locus_tag="OO135_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5398823.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1310..1831
                     /locus_tag="OO135_003064"
     CDS             1310..1831
                     /locus_tag="OO135_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5398824.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1867..3252)
                     /gene="cysS"
                     /locus_tag="OO135_003065"
     CDS             complement(1867..3252)
                     /gene="cysS"
                     /locus_tag="OO135_003065"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5398825.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3426..3920
                     /gene="ppiB"
                     /locus_tag="OO135_003066"
     CDS             3426..3920
                     /gene="ppiB"
                     /locus_tag="OO135_003066"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5398826.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3923..4645
                     /gene="lpxH"
                     /locus_tag="OO135_003067"
     CDS             3923..4645
                     /gene="lpxH"
                     /locus_tag="OO135_003067"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5398827.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4763..5272
                     /gene="purE"
                     /locus_tag="OO135_003068"
     CDS             4763..5272
                     /gene="purE"
                     /locus_tag="OO135_003068"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5398828.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5269..6336
                     /gene="purK"
                     /locus_tag="OO135_003069"
     CDS             5269..6336
                     /gene="purK"
                     /locus_tag="OO135_003069"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5398829.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6473..7366)
                     /locus_tag="OO135_003070"
     CDS             complement(6473..7366)
                     /locus_tag="OO135_003070"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5398830.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQIPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLATALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7363..8178)
                     /locus_tag="OO135_003071"
     CDS             complement(7363..8178)
                     /locus_tag="OO135_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR5398831.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8189..9448)
                     /locus_tag="OO135_003072"
     CDS             complement(8189..9448)
                     /locus_tag="OO135_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR5398832.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSACMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMVRGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLDERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKKAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9458..10811)
                     /gene="fdrA"
                     /locus_tag="OO135_003073"
                     /pseudo
     CDS             complement(9458..10811)
                     /gene="fdrA"
                     /locus_tag="OO135_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2373 a         3093 c         2895 g         2450 t
ORIGIN      
        1 taaccttcgc aagaggtcgc ttcacgcgat aaatctgaaa cgaaacctga cagcgcgccc
       61 cgcttctgac aaaataggcg catccccttc gacctacgta acagatggaa tcctctctct
      121 gatggcagca aagattattg acggtaaaac gattgcgcag caggtgcgct ctgaagttgc
      181 tcaaaaagtt caggcgcgta ttgcagccgg actgcgggca ccaggactgg ccgttgtgct
      241 ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg cttgtgaaga
      301 agtcgggttc gtctcccgct cttatgacct cccggaaacc accagcgaag cggagctgtt
      361 ggagcttatc gatgcgctga atgccgacaa caccatcgat ggcattctgg ttcaactgcc
      421 gttaccggcg ggtattgata acgtcaaagt gctggaacgt attcatccgg acaaagacgt
      481 ggacggtttc catccttata acgtcggtcg tctgtgccag cgcgcgccgc gtctgcgtcc
      541 ctgcaccccg cgcggtatcg tcacgctgct tgagcgttac aacattgata ccttcggcct
      601 caacgccgtg gtgattggcg catcgaatat cgtcggtcgt ccaatgagca tggaactgtt
      661 gctcgcaggt tgcaccacta ccgtgactca ccgtttcact aaaaatctgc gtcaccacgt
      721 agaaaatgcc gatctgttga tcgttgccgt cggcaagccg ggctttattc ctggtgactg
      781 gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa atggcaaagt
      841 tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta cgcctgttcc
      901 gggcggcgtt ggcccgatga cggttgccac gctgattgaa aacacgcttc aggcgtgcgt
      961 tgaatatcat gatccacagg gtgagtaaca tggcgacatt ttctttaggt aaacatccgc
     1021 acgttgagct gtgcgatttg ctgaaactgg aaggctggag cgaaagcggc gcgcaggcga
     1081 aaatcgcgat tgccgaaggc caggtgaaag tcgacggtgc ggttgaaacg cgcaaacgct
     1141 gcaaaatcgt tgccggtcag acagtgagtt ttgcaggtca cagcgtacag gttgttgcct
     1201 gatccctttc ccggcctgta tcaacaggcc ggttctcttt tcagactttt gccctctacg
     1261 ccccgttcat ccatgctgat agcatcgaca ttttgccgaa gcgaccatca tgccaaccgt
     1321 tattacgcac gccgccgttc ccctatgcat tggtttaggg ttgggttcga aagtcatccc
     1381 tccgcgtctg ttatttgccg gaatcatcct ggcgatgctg cccgacgccg acgtattgtc
     1441 gtttaaattt ggcgttgctt acggcaatgt ttttggtcat cgcgggttta cccattcgct
     1501 ggtgtttgcg tttgttgtcc cgctgttatg tgtgctgatt ggacgccgat ggttcagggc
     1561 tgggctgatt cgctgctggc tgtttttaac cgtctcgttg ctatcgcaca gcttgctgga
     1621 ttcggtaacc actggcggta agggcgttgg ctggctgtgg ccgtggtcag atgaacgctt
     1681 tttcgctccc tggcaggtga ttaaagtcgc tccgtttgca ttgtctcgtt acaccacgcc
     1741 gtacgggcat caggtgataa tttctgaatt gatgtgggtg tggttgccgg ggatggtgct
     1801 tatgggaatg ttgtggtggc gcaggcgata accggatgcg aaaactcgca tccggcaaca
     1861 gcgcaattac ttacgacgcc aggtggtccc ttgcgggcca tcttccagca cgatccccat
     1921 ctcgttaaga cggtcacgcg ccgcatctgc cgccgcccag tctttcgctt tacgggcatc
     1981 cagacgctgt tgaattaacg cttcaatctc agccacttcg ctgtcgtctg cctgcgcgcc
     2041 gctttgcagg aacgcttccg gttcttgctc cagcaggccc aatacagcgg aaagtttacg
     2101 caggtgagat gccattgcat tcgccgctgc catatcttct gctttcagac ggtttacttc
     2161 acgcgccata tcaaacagta cggaataggc ttccggggtg ttgaaatcgt cgtccatcgc
     2221 ttcaataaag cgcgcttcaa acgcttcgcc accggcaggc gcaacggttt tatctgtgcc
     2281 gcgcagcgca gtgtagagac gctccagcgc cgcacgcgtc tgcttcaggt tctcttcgct
     2341 gtagttcaac tggctgcgat agtggcccga catcaggaag taacgcacgg tttccgcgtc
     2401 gtagtatttc agcacatcgc gcacggtaaa gaagttaccc agcgatttgg acatcttctc
     2461 gcggtcaacc atcaccattc cggagtgcat ccagtagttc acatactgac catcatgggc
     2521 acaggtggac tgcgcgattt cgttctcgtg gtgcgggaac atcaggtctg aaccgccgcc
     2581 gtggatatca aagtggttac ccagctgctt gcagttcatg gccgaacatt caatgtgcca
     2641 gccaggacga cccgcgcccc acggagacgg ccagctcggt tcgccctctt tcgacatctt
     2701 ccacagaacg aagtccatcg ggttgcgttt gtcatcaacc acgtcaacgc gcgcgccagc
     2761 ctgcagctgg tcgagatcct gacgcgacag cacgccataa gtcggatcgg tcgggacgtc
     2821 gaacatcacg tcgccgttgt cagccacata agcgtgacct ttggcgatca gttgttcagt
     2881 gagttcaata atttctgcga tatggtgcgt cgcgcgcggc tccatatccg ggcgcagaat
     2941 gttcaaagca tcaaaatctt tgtgcatttc ggcgatcatg cgatccacca gcgccacaaa
     3001 gctttcgcca ttttcattgg cgcgtttgat gattttgtcg tcgatatcgg taatgttgcg
     3061 cacatacttc agcttatagc cgaggaaacg cagatagcgc gcaaccacgt caaaagcaac
     3121 aaaggtacgc ccgtgaccga tatgacagag atcgtaaacg gtgattccac acacgtacat
     3181 gccgacttcc ccggcgtgaa taggcttaaa ttcctctttt tggcgtgtca gagtattgaa
     3241 gatttttagc atcgaagatt ccgtttagac atgtgtgggt aattgagttg cgtataatac
     3301 ccatattttc cgcgcgaatc agcatacatt gcgtgatgat ccgatcgtgc ggttatgcta
     3361 taacaccacc ctatatatga cccgaaccgg gttgaagcac caatcaaacg gaacaggatg
     3421 caaaaatggt tactttccac accaatcacg gcgatattgt catcaaaact tttgacgata
     3481 aagcacctga aacagttaaa aacttcctgg actactgccg cgaaggtttt tacaacaaca
     3541 ccattttcca ccgtgttatc aacggcttta tgattcaggg cggcggtttt gaaccgggca
     3601 tgaaacaaaa agccaccaaa gaaccgatca aaaacgaagc caacaacggt ctgaaaaata
     3661 cccgtggtac gctggcaatg gcccgtactc aggctccgca ctctgcaact gcacagttct
     3721 tcatcaacgt ggttgataac gacttcctga acttctctgg cgaaagcctg caaggttggg
     3781 gctactgcgt gtttgctgaa gtggttgaag gcatggacgt ggtagacaaa atcaaaggtg
     3841 ttgcaaccgg tcgtagcggt atgcaccagg acgtgccaaa agaagacgtt atcattgaaa
     3901 gcgtgaccgt tagcgagtaa tcgtggcgac actctttatt gcagatcttc atctctgcgt
     3961 ggaagaaccg gcgatcaccg ccggttttct gcgtttttta gcgggggaag ctcgcaaggc
     4021 cgacgcgctg tatattcttg gcgatctgtt tgaagcgtgg attggcgacg acgatcccaa
     4081 tccactccat cgccagatgg cggcagcgat caaagcggtg tccgattccg gcgttccctg
     4141 ttatttcatt catggcaacc gtgattttct gctcggcaaa cgctttgccc gtgaaagtgg
     4201 catgacgtta ttgccggaag aaaaggtgct cgaactttat ggccgccggg tgttgattat
     4261 gcatggcgac acgctgtgca ccgatgacgc gggttatcag gcttttcgcg ccaaagtcca
     4321 caaaccctgg ctgcaaatgc tattcctcgc cctgccgttg tttgtgcgca aacgcattgc
     4381 cgcgcgaatg cgcgcgaaca gcaaagaagc caacagcagt aaatcgctgg cgatcatgga
     4441 cgttaaccaa aacgcagtgg tcagtgcgat ggaaaaacat caggtgcaat ggctgatcca
     4501 cgggcatacc catcgcccgg cggtgcatga acttatcgcc aaccagcaac ctgcttttcg
     4561 cgtggtactg ggtgcctggc atacggaagg ttcgatggtg aaagtcacgg cggatgacgt
     4621 tgagctgatt cattttccgt tttaaaaaaa ccacaacttt gctgatttca cagccacgca
     4681 accgttttcc ttgctctctt tccgtgctat tctctgtgcc ctctaaagcc gagagttgtg
     4741 caccacagga gttttaagac gcatgtcttc ccgcaataat ccggcgcgtg tcgccatcgt
     4801 gatggggtcc aaaagcgact gggctaccat gcagttcgcc gccgaaatct tcgaaatcct
     4861 gaatgtcccg caccacgttg aagtggtttc tgctcaccgc acccccgata aactgttcag
     4921 cttcgccgaa agcgccgaag agaacggtta tcaggtgatt attgcgggcg caggtggcgc
     4981 agcgcacctg ccaggcatga ttgccgccaa aacgctggtg ccggtgctgg gcgtgccagt
     5041 acagagcgcc gcactgagcg gtgtcgatag cctctactcc atcgtacaaa tgccgcgcgg
     5101 cattccggtg ggtacgctgg cgattggtaa agctggcgcg gcaaacgcgg cattactggc
     5161 agcacaaatt ctcgcgactc acgataaaga gctacaccag cgtctgaatg actggcgcaa
     5221 agcccagacc gacgaagtgc tggaaaaccc ggacccgcga ggtgcggcat gaaacaggtt
     5281 tgcgtcctcg gtaacgggca gttaggccgt atgctgcgtc aggcaggcga accgttaggc
     5341 attgctgtct ggccggtcgg gctggacgct gaaccggcgg cggtgccttt tcaacaaagc
     5401 gtgattaccg ctgagatcga acgctggccg gaaaccgcat taacccgcga gctggcgcgt
     5461 catccggcct ttgtgaaccg cgatgtgttc ccgattattg ctgaccgtct gactcagaag
     5521 cagcttttcg ataagctcca cctgccgacc gcgccgtggc agttacttgc cgatcgcagc
     5581 gagtggcctg cggtgtttga tcgtttaggt gaactggcga ttgttaagcg tcgcactggt
     5641 ggctatgacg gtcgcggtca atggcgttta cgcgccaatg aaaccgaaca gttaccggca
     5701 gagtgttacg gcgaatgtat tgtcgagcag ggcattaact tctctggtga agtgtcgctg
     5761 gttggcgcgc gcggctttga tggcagcacc gtgttttatc cgctgacgca taacctgcat
     5821 caggacggta ttttgcgcac cagcgtcgct tttccgcagg ccaacgcaca gcagcaggcg
     5881 caagccgaag agatgctgtc ggcgattatg caggagctgg gctatgtggg cgtgatggcg
     5941 atggagtgtt ttgtcacccc gcaaggtctg ctgatcaacg aactggctcc gcgtgtgcat
     6001 aacagcggtc actggacaca aaacggtgcc agcatcagcc agtttgagct gcatctgcgg
     6061 gcgattaccg atctgccgtt accgcaacca gtagtgaata atccgtcggt gatgatcaat
     6121 ctgattggta gcgatgcgaa ttatgactgg ctgaaattgc cgctggtgca tctgcactgg
     6181 tacgacaaag aagtccgtcc ggggcgtaaa gtggggcatc tgaatttgac cgacagcgac
     6241 acatcgcgtc tgactgcgac gctggaagcc ttaatcccgc tgctgccgcc agagtatgcc
     6301 agcggcgtga tttgggcgca gagtaagttc agttaagcag gtgctcattt gattgccgga
     6361 tgcggcgtaa acgccttatc cggcttaccg ctccggtacc cattgtaggc ctgataagac
     6421 gcgtcaagcg tcgcatcagg catggtgcac cgattgccgg aaactctcat cactacagcg
     6481 aaatacaggt ccccgcttcg cccgccagcg tctcttcaat tcgcgataac gccccaatcc
     6541 acgcgggttt actacggctt ctgacataac cactcaccgc cgttaccttc ggccccatcg
     6601 aaccatcggc tttggcaaat ggcgctaact catccggtgt ggcatggcga atggcacgtt
     6661 gctgcggcgt tccccagttt tcatataccg catcagcatc ggtgaggatc accagtccat
     6721 cggcattaat ctgctcggcg agcaacgcag tggcgagatc tttatcaatc accgcttcac
     6781 tccctgcccc atcttccgtc acaggcacac cgccgccgcc actgcaaatc accacatgcc
     6841 cctctttgag caacaactcg atggcttcgc tgtcgagaat tttacgcggt tgcggagacg
     6901 ccaccacccg gcgcaaatat ttaccatcac gtttcatctg ccagccgtaa gccgcttcca
     6961 gtgcctcttg ttcttctggc tgataaaccg gaccaataaa tttctctggc tgcaaaaacg
     7021 ccggatcatc aggcgaaacc tcaatgcgcg tcagcaccgt cgtaacaggc ggtatctgcg
     7081 gctgtgcgct caagctctgc gccagcatat agccaatcat cccctggctt tccgccacca
     7141 gcacatccag cggatacggt tctacctctt tccacgccag attctgtaac gccagcagcc
     7201 ccacctgcgg cccgttgccg tgaacaatcg ccagccgata agaacgggcc aggcgtgcca
     7261 gcgcgggtac agcactggcg atattgcgat attgattttc cgccgtcagc gcctcaccgc
     7321 gctggagtaa ggcgttgccc ccaagagcta caaccagtgt tttcatggtt ttccttgtaa
     7381 taattgttgg ccaagccaga accccagcag cgtatctgcc cctgacgtat gccccagcgc
     7441 cagcagcgaa tcaatcgcaa cagcggtacg tttcgggcaa ctcagagcat ggacaaagtg
     7501 caggagtggc gaggcgaaat atccttgcgc ggcataacgt aaataactga cgctcaccgc
     7561 tgtggtaacg agttgaagat tgtcggaaca ggcaaaaaac gggcgaccgg cgcgcgcatc
     7621 taaagcgcca taataccaag ccgccagcag cattccgctc aacgtgtcat catgacttgg
     7681 cgttaatccg gggcctttac ccagccagtg ccgccagtcg gtcttaacgc cattgagcgc
     7741 ggcctgaaaa cagtgacgaa actggcgtaa ttcagcggtc agcggatcgc ttgccgccat
     7801 cgccagtggt ccgaaaagtc cggtttcctc cgcgcgttgc atccatgcag ctgcaagcgg
     7861 ttgaggatgc gaaggcggcg taatacgcag caaacaataa cgctgtggct gtttaaccgt
     7921 gaaacgcccg aggcgaatcc cttgagccac aacctgtggt cgttcattgc cgcataatcc
     7981 accgcataac gcatcgaatt gcgcgcggcg aagcacccat cctccggggc cgaaaccact
     8041 accctgacga tgcagcgtta acagttcgcc gctttccgtc atcaggttaa tcgcccgccg
     8101 ccacactccc gctaaccgcc aggactggcg ataattcggt gcgctactgc tggcaagcag
     8161 aggatggatg atcgtcattg cgcgttcctc aaccaatccc catgctttca gccagtgctt
     8221 ccagcgcctg ttcaaagcac gccagcggtg cccgcacggt gcctgcgcca atctgcccga
     8281 tccccgcctt tttatgggcg ataccggtat tgatgagtgg cgtaatgccg gtttctacca
     8341 cgcgacgaat atccagcccc aggcacgcgc cctgaaaatc ccagcctggg atctgcaact
     8401 gcatattgcg ttcaaggtaa atttccgcca tctcttcaga taccgccctg gccgcttcca
     8461 tgccacccgc accgacaaag cgcgttacgc caggcgctgc gatcattgcc gcacctccga
     8521 taccaaaggt ttcggtaatc gcgctatcgc ccatatccgg gttcgcctgc tcctgcgaga
     8581 agccggtgaa aaacagacct tgcggagtgt ttacaggcgc agtaaaccag cgttcatcca
     8641 gcccgcttac ccgaatcccg aacatattgc cgttgcgggt catagccgta acgatgctgc
     8701 ctgcgcggat catcgcgcca gcatccatcg ccgccttgca gtaggccatc gcgaggttga
     8761 ggaagaactg atcggtcacg ctgaggaaat cgatcacttc cgcaatgtgc tgtttatcat
     8821 gatcgaggcg agcaatttgt ggggccagcg tacgcattaa cagtgctgag gaagcaatat
     8881 tgcgttgatg gaactcatcg cccatcgtaa tgccctgcgc catcatcgcc gtgagatcga
     8941 taccgcgcac catgcgcccc agcgccgcgc ttaatactgg cattaacaca tcgcgcatcc
     9001 agcgatggcg agtcaggaca tcttcgccgt aagcgccaaa acgcatcact ttgccgatac
     9061 cttcgttgag gttgcagtac gcccggttgc cgtcggttac gttctcgacc accagcatcg
     9121 gcatacaggc agaagtaatg ccgcccattg gcccgacggc attcacatgg tgacaaggaa
     9181 tgaagttcac ttccccctgc tccagcatcg ccagtgcctg cgcttcatct ttcgcccagc
     9241 cttcgaacag acatgcgccc acgcacgccc ctttcatggg tccggtcatc tcctgccagc
     9301 gcattggcgg cccggcgtga agcagtgttt tgccctcgtt tagttcgctg ataagtgaaa
     9361 aagccggttg cacatccagc cagtgtggac gagcacgacg aatttgttcg ataaccgcag
     9421 cattggcttg cgccactgat gtaaacatgg gaacccctta ttgcaaacgt tctaataaac
     9481 gagccagttt tttattgccg ccggcgactg gcgaccattg gtaatgcaca accggtttgc
     9541 tggcgctttg tagctccagc gcaaagctgc gtaatccgat gttaatcacg gcgacgtttt
     9601 ccagtaatga cggtgtatgt tgctgtgcgg caggcgagag cggataaatt aacgctgccg
     9661 ccagcaaggt ggcttccggc agcgaactca ctaccgcgat ccccgcatct tccagcgtgg
     9721 cgatttgctg cgagcggcat tgcgggtcac gttcagtgcc tgtcaccgtg gcaatggcat
     9781 acagtggttg attatctgaa cgcgcggcac aggctttttg ctaggcgctt accagcgagg
     9841 cggcaggatc ggcagtcgca ccgaagccaa tcacgacatc aagtagcaat acgcgcactt
     9901 gtggtttagc gccgagatcg gcaattaact ggttgcgtaa ggctgggtcg atcatcggat
     9961 ggggacgccc gacggtgtag aaatcatcgc cgaggtcaat aatctggtga ccatcggcgt
    10021 cgagcatcat gccgtgatga tgggtatcgt cggcttccac gccaaggtgt ccagcaagta
    10081 atcccgccgc ttcggcagcc agcgtaccgc cggtatacaa accgcaaata aatccgctgc
    10141 tgacaggcgc tattgcgtta cgtcgcgccg tgacgcgtga aagcaggcaa gccaggcgtg
    10201 cggcctcatc cagcgaggag gcaaaccaga cattctcgtc gcgggccacc gccggggtat
    10261 aacctaaaaa cagcgccacc gtcggtttgc cggttgcttt catggcatta acaattttca
    10321 gacgcacagc ttcggcaggt ggctttgaaa caaatgccag cacttcgctt ttctcgtctg
    10381 cactgagcat ttccagcgcc gtcagcgcac tgatgccgcc tgcttcacgg ctgaggtcgc
    10441 gctcgccaag accaatcgcg tgagtaattc cctcccctgc ctgcgcaatc tgcgaacaca
    10501 gttcctgaat cccggtaccg gaagcgccaa tgacgccaat gttgccttcc ggcatcacgt
    10561 tagcaaaagc cagcggcgtg ccggcaatca tcgacgtacc gcagtccggc cccatcacca
    10621 gcaagccttt ttctcgcgcg cgggttttaa gttggatttc atcttccagc gtgacgttat
    10681 cggagaacat catcacgttg aggttgcgat ccagcgcctg attcgccagc tccgccgcat
    10741 actcgccagc cactgaaatc agcgccagat tggcatcgga taatttctga caggcactgt
    10801 cccagcgacg c
//
LOCUS       DAJTMK010000135        10736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTMK010000135 DAJTMK010000000
VERSION     DAJTMK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.137"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..99)
                     /locus_tag="OO135_003074"
     CDS             complement(<1..99)
                     /locus_tag="OO135_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HCR5398833.1"
                     /translation="MLKQRRNFRTGTERHANRFTTSASRSNIRYSLS"
     gene            complement(114..392)
                     /locus_tag="OO135_003075"
     CDS             complement(114..392)
                     /locus_tag="OO135_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5398834.1"
                     /translation="MAKVLNTHEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     AVNTVKRQADRGSIPINQDEKNSRRTVNLFALFLKTIRSAEKYVQMTK"
     gene            489..797
                     /locus_tag="OO135_003076"
     CDS             489..797
                     /locus_tag="OO135_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5398835.1"
                     /translation="MLIGEKIRVIRESEDLTREEFCGLIDVPIGTLRRYETGRIENIG
                     GEVLIKIVNHPRFFKYMNWLMTGKTNEAAGQISPSLSPDGPENTSSSQKSRKTGTQPG
                     "
     gene            886..1824
                     /locus_tag="OO135_003077"
     CDS             886..1824
                     /locus_tag="OO135_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5398836.1"
                     /translation="MCPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALHHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAARFNPLGGA"
     gene            1827..2189
                     /locus_tag="OO135_003078"
     CDS             1827..2189
                     /locus_tag="OO135_003078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398837.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            2297..2626
                     /locus_tag="OO135_003079"
     CDS             2297..2626
                     /locus_tag="OO135_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398838.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
     gene            2835..3814
                     /gene="btuC"
                     /locus_tag="OO135_003080"
                     /pseudo
     CDS             2835..3814
                     /gene="btuC"
                     /locus_tag="OO135_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
     gene            3876..4427
                     /gene="btuE"
                     /locus_tag="OO135_003081"
     CDS             3876..4427
                     /gene="btuE"
                     /locus_tag="OO135_003081"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5398839.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCATTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            4427..5176
                     /gene="btuD"
                     /locus_tag="OO135_003082"
     CDS             4427..5176
                     /gene="btuD"
                     /locus_tag="OO135_003082"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5398840.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQHVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSRDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            5254..5718
                     /locus_tag="OO135_003083"
     CDS             5254..5718
                     /locus_tag="OO135_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5398841.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            5965..6678
                     /gene="ydiV"
                     /locus_tag="OO135_003084"
     CDS             5965..6678
                     /gene="ydiV"
                     /locus_tag="OO135_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5398842.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            6741..8177
                     /locus_tag="OO135_003085"
     CDS             6741..8177
                     /locus_tag="OO135_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5398843.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAELGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(8181..8372)
                     /gene="hemP"
                     /locus_tag="OO135_003086"
     CDS             complement(8181..8372)
                     /gene="hemP"
                     /locus_tag="OO135_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5398844.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(8504..9550)
                     /gene="aroH"
                     /locus_tag="OO135_003087"
     CDS             complement(8504..9550)
                     /gene="aroH"
                     /locus_tag="OO135_003087"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5398845.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(9707..10540)
                     /gene="ppsR"
                     /locus_tag="OO135_003088"
     CDS             complement(9707..10540)
                     /gene="ppsR"
                     /locus_tag="OO135_003088"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5398846.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         2633 a         2422 c         2840 g         2841 t
ORIGIN      
        1 actcaggctg tagcggatgt tgctgcgtga tgcactggta gtgaaacggt tagcgtggcg
       61 ttctgttccg gtacgaaaat tacggcgttg cttcagcata aaatgacacc tcgttatttt
      121 gtcatctgca cgtatttttc tgcgctcctg attgttttca ggaaaagagc gaagagattt
      181 actgtgcgtc ttgagttctt ttcatcttgg ttgatgggaa ttgaacctct gtcagcctgt
      241 cttttcactg tgttaacagc ttggttggta cgctttgcgt aatctttcag gctttcttca
      301 agtactggta acccatgctc atcgcggtag ggatagaacg ctgctaaacg ctcaaagtct
      361 gcctgttcgt gtgtgttcag gacttttgcc atgtgtgata acctgcgcta tctgtggttg
      421 tttgtgactt ggtgtactta taagtacacc ttgtgcgcaa gcttagtgta cttataggga
      481 cactgtcaat gcttatcggt gaaaaaatta gagtgattcg tgaatcagag gatttaacgc
      541 gcgaagaatt ttgcggcctg attgatgtgc ctatcggcac tttgcgtcgt tatgaaacgg
      601 ggcggattga aaacataggg ggcgaagtgc ttatcaagat tgttaatcac cctcgctttt
      661 ttaagtacat gaattggctt atgacgggaa aaacaaatga ggctgctggg cagatcagtc
      721 cctctctctc ccctgatggg ccagaaaaca catcgtcttc tcaaaaatcc cgcaagactg
      781 gtacacagcc cggctaatca tagaacgctg ggggcatggt ggtcttgtaa cgctggggtt
      841 tcacgaatga gcataaaatc aattccggga gggtatcttc ttgacatgtg ccctgagggg
      901 cgtaaaggca aacgcattcg caaaaaattt aaaacgaaat cggatgcagt tttatatgag
      961 cggtgggtgc tggcgcaaca gcataacaat gagtggaaag gaaactctat tgatcgccgt
     1021 ccactgtcag tgcttattga cttgtggtgg aaataccacg gccagctaat gaagtcaggg
     1081 cataacacgc gccttaaatt gctgcgcttg agtgaagcaa tggatgaccc gtgcgtgcat
     1141 aaacttaata caacgatgct caccgagcta cgtgtgtcca ggatagagca ggggatacag
     1201 cccagcacca taaatcgaga gattggggcg ttaagcgcga tgtttaccgc actcatctca
     1261 tccggccatt ttcttaacga taaccccgtt caaggcctta aaggaatgaa ggttaacgag
     1321 cgcgaaatgg gatatctgag taagtctgaa tgtgttcagt tgctggatgc actggctgaa
     1381 aatcccgatg aacgactggc tgtcgaaatc cttctgtcga ccggggcgcg atggggcgag
     1441 gtagcggcac tggagcagcg ccgtgttctt cattgtcgaa tcactttttc aaaaacgaag
     1501 aacagcaaaa accgtaccgt tcctatttct gaaagcctgt ttgaaaagat caaaaaacgg
     1561 ggcgggaaac tggtgtttcc gacgctggat tattcattgg ttcgcgatgt catcaaaacg
     1621 gtcgcacctg atgttcctga cggccaggct gttcatgcgc tgcaccacac cttcgccagt
     1681 catttcatga tgaacggcgg caatattctg acgctccaga aaattctggg gcacgcaaag
     1741 attcagacaa cgatgattta tgcccatctt gcgccggatt acctgcagga tgcggcgagg
     1801 tttaatccac taggaggtgc ttaagagtga agtttcattt agatgaactt aaaagaatgg
     1861 caacgctcga taattttgcg cgttttctca atgagtcgtc tacgaatgaa aaatgtttgt
     1921 cgtgtggcga tacggatatg tacatgtatt tgacgaatat tgttgaggtg gggcctgagc
     1981 cgaaaactgc tgaggagtgt gatttaggca cttttgttat gcttgattac atcggtccat
     2041 ttactgggta tccgggatat gagggacatg atcgggaaaa tatccataac tatgaattcc
     2101 gactgacctg taacagatgt gggtttgttc atcgttactc ggcccgggcc tttatgaact
     2161 gggtaggcaa gcagggtgat gagaagtgat ggcagagaat gttgcacata tttcgcggtt
     2221 cagaaaaaag aaagagaatg cctataatgt atccaacaac caagtaacag gaagtggtaa
     2281 tgatggaggt gatggcatga atgacgacct tgaacggcgg gtcagttgcc tggaaagtga
     2341 tgtgactgaa atcaaaaaca acctgattac tctcaccaca agaagcgaat cctttgctac
     2401 caaatctgat gtactggaga ttcgcgaagg gttgaggctt gagatggcag aatcgcgcca
     2461 atccctgaag tctgagatgg ctgatttacg ccagtctctg aaagttgaga tggctgagca
     2521 tcgtactgaa cttcagaaat catttgcaaa tcagacttgg ttactcacag gtattgtcct
     2581 gtctgcgatg gccgtgcttg tggctgtggt tactgttatt aagtaaattt atggcggcga
     2641 tatgatccac aaagtgacca catccctgtt atttgttgtg gttggctgtg tttttgtgtg
     2701 tctgtaagtc tttgataatt atctaacttg ttgattttta cttgtgttta tggccgctct
     2761 gcggcctttt ttcttttcac tgtcgaagag tcaccgtaaa atcaacgcca tgacacttca
     2821 gcagaacgga taccatgctg acacttgccc gccaacaaca gcgacaaaat attcgctggt
     2881 tattatgcct gtcagttttg atgctgctgg cgcttctctt aagcctttgc gccggtgaac
     2941 aatggatctc gctaggtgac tggtttactc ctcgtggcga actgttcgtc tggcaaattc
     3001 gcctgccacg tacgctggct gtattgctgg ttggtgcggc gctggctata tccggcgctg
     3061 taatgcaggc gttgtttgaa aatcctctgg cagaacctgg actacttggc gtctctaacg
     3121 gcgcaggcgt ggggcttatc gccgcggtat tgcttgggca agggcaactc cccaactggg
     3181 cgctagggct gtgtgcgatt gctggcgcgc ttatcatcac tttaatactc ttacgtttcg
     3241 cccgtcgtca tctttcgacc agtcggttat tgctggctgg cgttgcatta gggattatct
     3301 gtagcgcact aatgacgtgg gctatctact tttccacctc tgttgattta cgtcagctga
     3361 tgtactggat gatgggcggt tttggcggcg tagactggcg gcaaagctgg ctgatgctgg
     3421 cattgatccc cgtgttgttg tggatctgtt gtcagtccag gccgatgaat atgttagcac
     3481 ttggcgagat ctcggcgcgg caactgggtt tacccctgtg gttctggcgc aatgtgctgg
     3541 tggccgcgac cggctggatg gttggcgtca gtgtggcgct ggcgggtgct atcggcttta
     3601 ttggtctggt gatcccacat attcttcggt tgtgtggttt aaccgatcat cgcgtattac
     3661 ttcccggctg cgcgctggca ggggcgagcg cattgctgct ggccgatatt gtagcgcgcc
     3721 tggcattagc tgcccagagc tgcctattgg cgtggtcacc gcaacgttgg gtgcgccggt
     3781 gtttatctgg ttattgttaa aagcaagacg ttagccgcaa aaagacggtc tatgattaaa
     3841 agctaagatt tttctgacca cacccaggag aaacgatgca agattccatt ctgacgaccg
     3901 tagtgaaaga tatcgacggt gaagtgacca cgctggagaa gtacgccggt aatgtgctgt
     3961 tgattgtcaa tgtcgcctca aagtgtggct taacgccgca atatgagcag ttggagaata
     4021 ttcagaaagc ctgggccgat cgaggttttg tggtgctggg attcccgtgc aaccagtttc
     4081 tggaacaaga accgggcagc gatgaagaga ttaaaactta ctgtgccacc acatgggggg
     4141 tgacgttccc gatgttcagt aagattgaag ttaacggcga aggacgccat ccgctgtatc
     4201 aaaaattgat tgccgcagcg ccgaccgcag tcgcgccgga agagagcgga ttctatgccc
     4261 gtatggtcag caaaggtcgt gcaccgctgt accctgatga tattttatgg aattttgaga
     4321 aatttttagt tggcagggac ggattggtca tccagcgttt ttccccggat atgacgccgg
     4381 aagaccccat tgtgatggaa agcattaaac tggcgttggc aaaataatgt ctattgtgat
     4441 gcagttacag gatgttgcgg aatctacccg cctggggcca ctttctggcg aggttcgggc
     4501 tggggagatc ctgcacctgg tggggccgaa tggcgcgggt aagagtacct tgcttgcacg
     4561 aatggccgga atgaccagcg gtaagggaag cattcagttc gcggggcaac ccctggaagc
     4621 atggtccgca acaaaactcg cgctgcatcg cgcctatctt tcacaacagc agacgccgcc
     4681 gtttgcaatg ccggtctggc actacctgac actgcatcag cacgataaaa cgcgtaccga
     4741 actactgaat gatgtcgcag gggcgctggc tcttgatgac aaactcggtc gtagcaccaa
     4801 tcaactttcc ggcggtgaat ggcaacacgt acgtcttgct gcggtggtgt tgcaaatcac
     4861 accacaagcc aatcccgcag gccaattgct gcttcttgat gagccgatga acagtcgtga
     4921 tgttgcgcaa caaagtgcgt tagacaaaat tctgagcgcg ctgtgtcagc aaggactggc
     4981 gattgtgatg agcagtcacg atctcaacca cacgttgcgt catgcgcatc gggcgtggtt
     5041 gctaaaaggt ggaaaaatgc tggccagtgg acgcagagaa gaggtgctca cgccgccaaa
     5101 tctggcgcag gcctatggga tgaattttcg ccgtctggat atcgaaggtc acagaatgct
     5161 gattttgacc atctgatttc attagtatct tgaaaatcgg gtaattacga cgctaaatta
     5221 atgcaagaat aaaaaacaga ggaaccgtac ggaatgcgtt tctgccttat tttgatcaca
     5281 gcactgtttc tggccgggtg tagccaccat aaagcaccgc cgccaaatgc cagactttct
     5341 gattcgatta ccgttattgc cggtttgaac gaccagctac aaagctggca tggcacgccg
     5401 tatcgttatg gtggcatgac gcggcgcggt gtggactgtt cgggatttgt ggttgtgacg
     5461 atgcgcgatc gtttcgattt gcagctgccc cgtgaaacca aacaacaagc ctctattggc
     5521 acgcaaattg ataaagacaa gttgctgcca ggtgacctgg tctttttcaa aacggggtcc
     5581 ggacaaaatg gtttgcatgt aggtatttac gataccaaca accaatttat ccacgcctct
     5641 accagcaagg gagtgatgcg ttcctcactt gataatgtct attggcagaa aaatttctgg
     5701 caggcgagac gaatctagta tgtcgaaaaa tggacggcga tgatacatcg ccgtccattt
     5761 actcctgaat acagtagtat tttgcagaca aaaattccgt aaatggatta ttcgtttata
     5821 atcaggataa tctaaaaaaa ggacccctga aaaagtggtt aacatggaac aaatgaaagt
     5881 tactccttct gtaattgtta tgtcttatgg cctcatccct tttagccgga tactgaaaaa
     5941 catccttcga gagggacggt taccatgaag atttttttgg agaatcttta tcattctgat
     6001 tgctatttcc tgccgataag agataaccag caagtccttg tcggcgttga gttaattacc
     6061 catttctcca gtgaagatgg cacggtgcgt atcccgacga gtcgggttat agcgcaactt
     6121 accgaggagc aacattggca attattttca gagcagttgg aattactaaa atcatgccaa
     6181 cattttttta ttcaacataa actctttgcc tggcttaatt taacgccaca agtggctaca
     6241 ttattattag agagggataa ttacgcaggc gaactattaa aatatccgtt tattgaactg
     6301 ttgattaatg agaattaccc gcatctcaat gaaggaaaag ataatcgagg tcttttatcc
     6361 ttatcgcaag tgtatcccct tgtgttaggt aatttgggag caggcaatag cacaatgaaa
     6421 gcggtttttg atggtctttt cacccgcgtc atgctggata aaagttttat tcagcaacaa
     6481 atcacgcacc gctccttcga accgtttatt cgtgcgatcc aggcgcaaat ttccccttgt
     6541 tgcaattgca tcattgcggg aggcattgat acggcagaaa tattggcaca gattaccccc
     6601 ttcgatttcc atgctctcca gggatgcctg tggcctgccg ttcccataaa tcagataacg
     6661 acgttggttc agcgataacc cttctgtttg ctggtgttta agacgagagt aaccgtctac
     6721 actatcaaac aggaggatct atgaccctgt cttttgttac ccgctggcgc gatgaattgc
     6781 cagaaaccta tacagcactt tcccctacgc ctttaaataa tgcccggctg atttggcata
     6841 ataccgaact ggctaacacg ctgagtattc catcgtcgct gtttaaaaat ggcgcaggtg
     6901 tctggggcgg cgaagcccta ctgcctggta tgtcaccact ggcgcaggtt tacagtggtc
     6961 atcagttcgg cgtctgggcg ggccaactgg gtgatgggcg cggcatttta ctcggcgaac
     7021 aactgcttgc tgatggcact acaatggact ggcatctgaa aggtgctggc ctgacgcctt
     7081 attcgcgaat gggtgatgga cgggcggttt tacgttcgac gatacgagaa agtctcgcca
     7141 gtgaggcgat gcattatctg ggcattccga cgacccgcgc gttaagtatc gtcaccagcg
     7201 attcgccagt gtatcgggaa acggcggagc taggcgcgat gctgatgcgt gtggcaccaa
     7261 gtcatctgcg ctttggtcat ttcgaacatt tttactaccg ccgcgagtcg gaaaaggttc
     7321 gtcagttagc tgactttgct attcgtcatt actggtcaca tcttgaagat gatgaggaca
     7381 aataccgtct ctggtttagc gatgttgtcg cacgtaccgc gtcgttaatt gcccaatggc
     7441 agacggtcgg ctttgcacat ggggtgatga ataccgacaa catgtcgctg ctgggactga
     7501 cgcttgatta cgggccgttt ggttttcttg atgattacga acccggtttt atttgtaatc
     7561 actcggatca tcaagggcgt tatagctttg ataatcaacc tgctgtcgcg ttgtggaatt
     7621 tacagcgtct ggcgcagaca ttgtcaccat ttgttgccgt agatgccctg aatgaggccc
     7681 tggacagcta tcagcaggtt ttgttgacgc attatggaca acggatgcgg cagaaactgg
     7741 gcttcatgac ggagcaaaaa gaggataacg cgctactgaa tgaattattc agtctgatgg
     7801 cgcgagagcg cagcgattat acccgcacat tccgcatgct gagtctgacc gagcagcaca
     7861 gcgcggcgtc accgctacgt gatgagttta ttgatcgtgc ggcatttgat gactggtttg
     7921 cccgttatcg ggggcgtttg caacaagacg aggttagcga tagtgagcgt cagcaactga
     7981 tgcaaagcgt taacccagct ctggtattgc gcaactggtt ggcgcaacgg gcgattgaag
     8041 cggcagaaaa gggtgatatg atggaattgc accgcctgca tgaggcgctg cgaaatcctt
     8101 tcagcgacag agatgatgac tatgtcagtc gtccacctga ctggggtaaa cggctggaag
     8161 tcagctgctc gagttaaagg ctacttagtt aacagcagct tgccagcctg agttttacgg
     8221 agcagatatt cttgcccgtc atgatcgata atcagtttgc catccggacc taacagtgtc
     8281 tggctggtaa ttcgccggat gggctgcggg gaagcggtct tgtgattggc atccgtttca
     8341 ggcgtgagtt ttctgctatc cgtataacgc ataaaaatta ataataattg atataacaat
     8401 gattcttatt atcaatatta tggcaggcca gagcaagcag taatgtgttg agaagacaac
     8461 aagttgcgca gaaatgccca tcccatcagg aatgggcacg cattcagaag cgggtatcta
     8521 ccgcagaggc gagtttttcg accaggcgtt cggtatcttc ccagcccaga cacgggtcgg
     8581 taatggattg accgtaagtg agcggctgac cgccgacgat tttttgcgtt ccttcgcgca
     8641 ggaaactttc cgccataatt ccagcaatcg ccgtagagcc attgcggatt tgctgacaaa
     8701 tatcctcaca aacttctaac tggcgacggt gctgcttctg gcagttaccg tggctgaaat
     8761 ccaccaccag atgttcaggt aaatcaaact cgtgcagcgt atcgcaggct gcggcgatat
     8821 catcggcatg ataattcggt tttttgccgc cacgcataat aatgtggcca tacgggttgc
     8881 cgctggtctg atagatggtc atctgaccat ttttgtctgg cgagaggaac atatggctgg
     8941 cgcgggctgc gcggatagca tccacggcaa tccgcgtgtt gccatcggta ccatttttaa
     9001 aacctaccgg acaggagagt gctgaagcca tttcgcggtg gatctgactt tcggtagtac
     9061 gagcgccaat cgcgccccaa ctgattaaat cagcaataaa ctgaccggtc accatatcga
     9121 gaaactcagt cgcggtaggg acacccagct catttacctg taaaagtaat ttgcgcgcca
     9181 gctccagacc gtgatttacc cgatagctgc cgtttaaatc cggatcggag attagccctt
     9241 tccagccgac aacagttcgt ggtttttcaa aataggtgcg cattacgatt tccagccgtg
     9301 actggtactg gttgcgcagc gactgcagac gggtggcgta ctccattgca gcggtgagat
     9361 cgtggatcga gcaggggcca ataatgacca acagtcgctt atcttcacca ttcagtattt
     9421 tttcaattct gcggcgggag tcggtgacat gggtggcgac gccaggcgtt acgggatacc
     9481 gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg cgcagtacgg agttcgtcag
     9541 ttctgttcat tacaagtctc agaaatttgt tgcttctacg gcaggactac cgggaagtga
     9601 tgcgcatcac gataaaccaa tcgcagctga tttcaagtgc gaggtgtgtc acacctcgcc
     9661 gggtggttag catggtaaca aaaaaacaaa ccaacgcact agttctctag tacattcggc
     9721 gactaaggcc catgatatcg aggatcttgg tggcaatctc ttctaccgaa taattggtac
     9781 tgttaatcca cgggatctga tttttacggt acaaggcttc cacttccgcg acttccattc
     9841 tgcactgacg aagcgaggca tagcgactgt tctcccgacg ttcctcgcga atcgccgcca
     9901 gacgttccgg gtcgatagtc aggccgaaca atttatgctg aagtggtttg agcgacgcgg
     9961 gtagcaccag attatccata tcgtcggcaa taaaggggta gtttgccgtg cggataccaa
    10021 attgcattgc cagatacaga ctggtggggg ttttaccaca gcgagaaaca ccgagcagga
    10081 tcacctgagc ctgatccaga ttgcgcaacg aaatgccgtc atcgtgggca agggtgtaat
    10141 caatcgccgc aatgcgcgca tcatatttat tgagattatt agggttaagg ccatgggtac
    10201 gatgagcaat cggcgttgga tccagtttca tctcttgttg tagcggggca accagcgcct
    10261 gaacgatatc ctggcaaaag ccttcacttt gcaagatgat ggcgcgaatc tccggcaaca
    10321 cgatggagta gaagaccagc gggcgcacac ctgtctggtg ataaattgcg tcaatctgat
    10381 ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg cagcgtaatg ctgctgatag
    10441 tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc cgcagttatt gccgtaccat
    10501 cagaaatata aaaaacgtgg cgatcaacag cattatccat tttgttcttc ccgtgatgca
    10561 gacataattc tatgaaagca taaattaaaa acgcacagaa gcgtaggagg taatgtctgg
    10621 tttataaaat gaaccttcaa ttttattttt tatgaaaaca gcatttcatt tttatggttt
    10681 cgtttatacc gatggtttat gtggaaattg tcgaatagag caggtttgcg caacgc
//
LOCUS       DAJTMK010000136        10655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTMK010000136 DAJTMK010000000
VERSION     DAJTMK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10655
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.138"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..576)
                     /locus_tag="OO135_003089"
     CDS             complement(<1..576)
                     /locus_tag="OO135_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5398847.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYA"
     gene            1143..1508
                     /locus_tag="OO135_003090"
     CDS             1143..1508
                     /locus_tag="OO135_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5398848.1"
                     /translation="MKKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1548..2243
                     /locus_tag="OO135_003091"
     CDS             1548..2243
                     /locus_tag="OO135_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR5398849.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQRNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            2310..3728
                     /gene="dcm"
                     /locus_tag="OO135_003092"
     CDS             2310..3728
                     /gene="dcm"
                     /locus_tag="OO135_003092"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5398850.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQIVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3709..4179
                     /gene="vsr"
                     /locus_tag="OO135_003093"
     CDS             3709..4179
                     /gene="vsr"
                     /locus_tag="OO135_003093"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5398851.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4168..5088)
                     /gene="yedA"
                     /locus_tag="OO135_003094"
     CDS             complement(4168..5088)
                     /gene="yedA"
                     /locus_tag="OO135_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5398852.1"
                     /translation="MRFRQLLPLFGTLFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFSIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            5261..6178
                     /locus_tag="OO135_003095"
     CDS             5261..6178
                     /locus_tag="OO135_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5398853.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            6257..6439
                     /locus_tag="OO135_003096"
     CDS             6257..6439
                     /locus_tag="OO135_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5398854.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6610..8304
                     /gene="yedQ"
                     /locus_tag="OO135_003097"
     CDS             6610..8304
                     /gene="yedQ"
                     /locus_tag="OO135_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR5398855.1"
                     /translation="MQHETKMENQSWLKKIARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQCNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFSHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8301..9116)
                     /locus_tag="OO135_003098"
     CDS             complement(8301..9116)
                     /locus_tag="OO135_003098"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR5398856.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9414..9641)
                     /gene="yodD"
                     /locus_tag="OO135_003099"
     CDS             complement(9414..9641)
                     /gene="yodD"
                     /locus_tag="OO135_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5398857.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9804..9992
                     /gene="dsrB"
                     /locus_tag="OO135_003100"
     CDS             9804..9992
                     /gene="dsrB"
                     /locus_tag="OO135_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5398858.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10036..>10655)
                     /gene="rcsA"
                     /locus_tag="OO135_003101"
     CDS             complement(10036..>10655)
                     /gene="rcsA"
                     /locus_tag="OO135_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5398859.1"
                     /translation="TIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACDSQ
                     RPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKILLIS
                     SKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISDQM
                     NIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
BASE COUNT         2724 a         2514 c         2749 g         2668 t
ORIGIN      
        1 cgcatagcca tcaccgtttt gtttagtaac gccacgattg ctgttcttac cttgatattg
       61 cacagcaaaa ttcagaccat caaccagacc aaagaaatca gagttacggt aggttgcaac
      121 accgttggta cgaccgttca tgaagttgtc agcggaagcc caggaatcac caccccattc
      181 aactaacata tcggtataag ctgcggcatc ataagcaaca ccgtagttac gaccatagtc
      241 gaaagatcct aactcgttat atttcaaacc tgcgaaggtt aaacgggttt tttcctgatt
      301 agagccttcg ggcttgctgg cgtcgaggtt gtattcaaac tgaccaaaac cagtcagttc
      361 gctggtgatt tgggtttcac ctttaacacc gaaacgagca taggaggtat cttcgttttc
      421 gctgttatta tcatcagtat ttgaccagat gcgttcgcca accatcttgc cgtagaaatc
      481 aactttattg ccatctttat tgtaaatttc agccgcattt gctgcgccag caactaataa
      541 cgccgggacc agcattgcca gaacttttct tttcattatg tattcccttg tgattataat
      601 cttcatgaat atatcaataa gtgccgttat ccaaaaaaaa gcacatttgg atactattct
      661 atgaagttca ttttatttta agcattacat gcaacaaatg tatttaaaag atttcaaaat
      721 gtttctaatc tatttaatat aattcagaat tatgaattct aaatataaat attcatgaaa
      781 attataaatg tatatcaata tgttatgagt tgagcggaaa catagggggt atcaatgatt
      841 gttgcgccaa acggctggtg tgatcaggcg cacattaatg aaggcataaa aaaaagccgg
      901 catcatgccg gcccaaaaaa tataaacaag acgataaaag gctaataacg gaagcatcat
      961 gacacagaat aataaaaagg tataattgta atcagtgcta atatattgat taaatgaatt
     1021 aataaagccg atatcaaaga taaataaaat cgctcaaaac gatttttcct gtattcactg
     1081 ccgttgcgca aaatttatct atttgttcaa aaaatgattg agtcttgact ggctcatcca
     1141 atgtgaaaaa atgtgacttt tatcacataa tagtactaag tctgaatttt ccgggttatc
     1201 tcaaaatgga atacggttcg acaaagatgg aagagagact ctctcgcagc cctggcggca
     1261 aactggcttt gtgggctttc tatacatggt gtggctattt cgtctgggca atggcgcgtt
     1321 acatctgggt gatgagccgg atccccgatg cccccgtgtc gggatttgaa agcgatttag
     1381 gctctaccgc gggaaagtgg ttaggggcac tggtcggatt tttatttatg gctctggtcg
     1441 gggcattgtt aggcagcatt gcctggtaca cccgacctcg tcccgcacgt agcagacgct
     1501 atgaataaaa actgctaagg tggcgactct ttggcaacgg agcgcgtatg gacttacaac
     1561 actggcaggc acagttcgaa aactggttga aaaaccatca tcagcaccag gacgcggccc
     1621 atgacgtgtg tcattttcgc cgcgtctggg ctacggcaca gaagctggcg gcagatgacg
     1681 atgtcgatat gctggtgatt ttaaccgcct gttattttca cgatattgtg agcctggcga
     1741 aaaatcatcc gcaaaggcag cgttcttcaa tcctggcggc agaagagacg cgtcgcctgc
     1801 tccgtgaaga gttcgagcaa tttccggcgg agaaaatcga ggccgtttgt catgtcattg
     1861 ccgctcacag tttcagcgcg caaatcgccc ccttaacaac ggaagctaaa atcgtccagg
     1921 atgcggatcg gctggaagcc ctgggggcga ttggcctggc gcgcgtgttt gccgtttcag
     1981 gggcattggg cgtggcgctg tttgatggtg aagatccgtt cgcacagcat cgcccgcttg
     2041 atgataaacg ctacgcgctg gatcatttcc agactaagtt gctgaagcta ccgcaaacca
     2101 tgcaaaccgc aagaggcaag cagctggcgc agcgcaacgc gcatttttta gtcgagttta
     2161 tggcgaagct cagtgccgaa ctggcggggg agaatgaagg tgtcgatcac aaggtgatag
     2221 atgcgttttc acccgcggga taagcgcgtg gccctaaatg gctgtaatta tgttaacctg
     2281 tcggccatct cagatggccg gttaaatcta tgcaggaaaa tatatcagta accgattcat
     2341 acagcaccgg gaatgccgca caggcaatgc tggagaaact gctgcaaatt tatgatgtta
     2401 aaacgctggt ggcgcagctt aatggtgtgg gtgagaatca ctggagcgcg gcaattttaa
     2461 aacgtgcgct ggcgaatgac tcggcatggc accgtttaag tgagaaagag ttcgcccatc
     2521 tgcaaacgtt gttacccaaa ccaccggcac atcatccgca ttatgcgttt cgctttatcg
     2581 atctatttgc cggaattggc ggcatccgtc gcggttttga atcgattggc ggacaatgcg
     2641 tgtttaccag cgaatggaac aaacatgcgg tacgcactta taaagccaac cattattgcg
     2701 atccggcgac gcatcatttt aatgaagata tccgcgatat caccctcagc cataaagaag
     2761 gcgtgagtga tgaggcggcg gcggaacata ttcgtcaaca cattcctgaa cacgatgttt
     2821 tactggccgg tttcccttgt cagccatttt cgctggctgg cgtatcgaaa aagaactcgc
     2881 tcgggcgggc gcacggtttt gcctgcgata cccagggcac gctgtttttt gatgtggtac
     2941 gcattatcga cgcgcgtcgt ccggcgatgt ttgtgctcga aaacgtcaaa aacctgaaaa
     3001 gtcacgacca gggtaaaacg ttccgcatca tcatgcagac gctggacgaa ctgggctatg
     3061 acgtggctga tgcagaagat aacgggccgg acgatccgaa aatcatcgat ggtaaacatt
     3121 ttctgccgca gcaccgtgaa cgcatcgtgc tggtgggttt tcgtcgcgat cttaatctga
     3181 aagccgattt tactctgcgt gatatcagcg aatgtttccc tgcacagcga gtgacgctgg
     3241 cgcagctgtt ggacccgatg gtcgaggcga aatatatcct gacgccggtg ctgtggaagt
     3301 acctctatcg atatgcgaaa aaacatcagg cgcgcggtaa cggcttcggt tatggaatgg
     3361 tttatccgaa caatccgcaa atcgtcacgc gtacgctgtc tgcgcgttat tacaaagatg
     3421 gcgcggaaat tttaatcgat cgcggctggg atatggccaa aggtgagaaa gactttgacg
     3481 atccgctgaa tcagcaacat cgtccacgtc ggttaacgcc tcgggaatgc gcgcgcttaa
     3541 tgggttttga agcgccggga gaagcgaaat tccgcattcc ggtttcggac actcaggcct
     3601 atcgccagtt cggtaactcg gtggtcgtgc cggtctttgc cgcggtggca aaactgcttg
     3661 agccaaaaat caaacaggcg gtggcgttgc gtcagcaaga ggcacaacat ggccgacgtt
     3721 cacgataagg ccactcgcag caaaaatatg cgcgcgattg ccacgcgtga tacggcgatt
     3781 gagaagcgcc tcgccagtct gttaaccggg cagggcctgg cgtttcgcgt tcaggacgcc
     3841 agtctgcccg gaagcccgga ttttgtcgtt gatgaatatc gctgcgtgat atttacccat
     3901 ggctgcttct ggcatcatca tcactgctat ctgtttaaag tgcctgcgac tcgaaccgag
     3961 ttctggctgg agaagatagg taaaaatgtt gaacgcgatc gccgcgatat cagtcgtttg
     4021 caggaactcg gctggcgcgt actgattgtc tgggagtgtg cattacgtgg gcgcgagaag
     4081 ctgacggatg aagcgcttac cgagcgtctg gaagagtgga tctgcggcga aggtgccagc
     4141 gcgcagatcg acacgcaggg gattcattta ctcgcttgat gcgtcctgaa taactggcgc
     4201 aactacgggt tttgccggga agagatattt tcccaacgtg accagtacca ccgcgaagac
     4261 aattacgccg agcgccagcc attcaatctt cgacagcgtc tctccgccca gtcccgtacc
     4321 cagcaagacc gcgaccaccg ggttaacgta ggcgtagctg gtggcgagag ccggactgac
     4381 attacggatt aaatacatat aagcgttgat ggcgataatc gaaccaaaca gcgccagata
     4441 gccgaccgca aggaagcctg aaagggaagg gagcgccgtc agtttttcac ctgcaatcat
     4501 cgacgcgatc attaacacca cgcctgccgc cagcatctca atcgcacccg ccatcatccc
     4561 tacaggtaag gtaatgcgcg agccataaac cgagccaaac gcccagctaa tcgagccgat
     4621 taaaatcaga atcgcgcccc acggattgcc gcttaaattt ccaccgctat tgagcatgat
     4681 gattccggca agcccaatgg cgatacccac ccattccaat ttgcgcgttt taatgctaaa
     4741 caggcggctg aagcacaggg taaagagggg cacggttgca accacaacgg cggcgatgcc
     4801 ggaaggaaca ttttgatgct cggcaaccgt caccatgcca ttaccgacag ccagcaataa
     4861 caggccaatc agcgcggcat tgagcagcgg acgtagcggg gggagcttgt gtccgcgcag
     4921 tagcaaaaat gccatcaata aaataccggc tgccaggaat cgaacgcctg ccatcattaa
     4981 cggaggccag ctttccacgc caatccgaat gacaaaatag gttgagcccc aaatgatata
     5041 caacgcaaac agcgtgccaa aaagcggtaa caactggcgg aaacgcataa tccctcacgg
     5101 tagaaataaa aaggtggttc atagtaaacg tgaaaatcat tctgctggcg agagatataa
     5161 ttgcacttga ttgttaaaaa aatgttgacc tgtgaagcaa gtatcagagc tgccgttttt
     5221 gcttcatact tacacccttc aacaataaaa atgagaggga atgcttttgg ccgggagtag
     5281 tttactgacg ttgctcgatg atatcgccac actactggac gatatctccg tgatgggcaa
     5341 actggcggcg aagaaaaccg ccggtgtatt aggggatgac ttatcgctca atgcgcaaca
     5401 agtttcaggc gtgcgggcca accgggaact tcccgtggtc tggggcgtgg cgaaaggatc
     5461 gctgattaat aaagtgattc tggtgccgct ggcgctgatc atcagtgcgt ttatcccgtg
     5521 ggcgattacg cctctgttga tgattggtgg cgcgtttctc tgctttgaag gagtagagaa
     5581 agtgctgcat atgctggagg cgcgtaaaca taaagaagat ccggcgcaga gccagcagcg
     5641 tctggagaag ctggcggcgc aggatccgct gaagtttgaa aaggacaaaa taaaaggggc
     5701 gattcgtacc gattttatat tgtctgctga aatcgtcgcc atcacgctgg ggattgtggc
     5761 cgaagcgccg ttgcttaatc aggtgctggt gctttcaggc atcgcgctgg tagtgaccgt
     5821 gggcgtctac ggtctggtag gggttatcgt taagattgat gacctggggt attggctggc
     5881 ggaaaaatcc agcgcgctga tgcaggcatt aggtaaaggg ttattgatca ttgcgccctg
     5941 gctgatgaaa gcgttatcga ttgtcggcac gctggcgatg ttcctcgtcg gcggcgggat
     6001 tgtggtacat ggtattgcgc cgctgcatca cgccattgaa catttcgccg ggcagcaaag
     6061 tgcagtggtg gcgatgatac taccgactgt tttaaatctg attcttggat ttatcatcgg
     6121 cggcatcgtg gtgctgggag tgaaagccgt agcgaaaata cgcggtcagg cacattaata
     6181 ctcgcacagg ctattatgca aaattcgcca tcttcaacta aggtgaacaa gtttcactcg
     6241 aaaaaaggag gcgagtatga gctttatggt tagtgaggaa gttacggtta aagagggcgg
     6301 cccgcggatg attgtcaccg gatactccag cggtatggtt gagtgtcgtt ggtatgacgg
     6361 ttacggggtc aagcgggaag cttttcatga aaccgagctt gttccggggg aggggagtcg
     6421 ttctgcggaa gaagtttgaa agggtatcat taacaccagg ccggatcact tccggcctgg
     6481 tgtgccaatg cctgcgatga atgatagtcg cgtcttatca tgccaccatt ataaaattat
     6541 cgctgttttg cggtacgcta tcgccaggtt atgccagaat cataaaaaag caggttggga
     6601 gtcgtcaggg tgcagcacga gacaaaaatg gaaaaccaga gctggttgaa aaaaatcgca
     6661 cgccgcctgg ggcctggtca tgtcgttaat ctctgcttta tcgtggtatt gcttttttcc
     6721 accttgctga cctggcgtga agtggtagtg ctggaagatg cctatatctc cagccagcgt
     6781 aatcatctgg aaaacgtagc caacgcgctc gataagcatt tgcagtgtaa cgtcgacaaa
     6841 ctgatctttt tgcgtaatgg catgcgcgaa gctctcgtag cgccactgga tttcacttca
     6901 ctgcgtaatg ctgtaaccga gttcgaacag catcgcgacg agcacgcctg gcaaattgaa
     6961 ctcaaccgac gacgcaccct gtcagtcaat ggcgtatcgg atgcattagt cagcgagggg
     7021 aatctcctgt ctcgcgaaaa tgaaagcctc gacaatgaaa ttaccgctgc actggaagtt
     7081 ggttacttgc tgcgactggc gcacaacacc tcgtcgatgg ttgaacaggc gatgtatgtc
     7141 tcgcgtgccg gattttacgt ttcgacgcag ccgaccttgt ttacgcgcaa tgtaccaacg
     7201 cgttattacg gctatgtcac ccaaccctgg tttatcggcc attcgcaacg agaaaatcgt
     7261 caccgcgcgg tacgctggtt tacttcgcaa ccggaacacg ccagcaatac tgaaccgcag
     7321 gttaccgtca gtgttccggt agacagtaat aactactggt atggcgtgct ggggatgagt
     7381 attcccgtgc gtaccatgca gcaattttta agaaacgcca tcgataaaaa cctcgatggt
     7441 gagtatcagc tctatgacag taagctgaga tttttgacct cttccaatcc tgaccatcca
     7501 acagggaata tttttgatcc tcgtgaactg gccttgctgg cgcaggcgat ggaacatgac
     7561 acgcggggcg gcattcgtat ggacagtcgc tatgttagct gggaacgtct ggaccatttc
     7621 gacggtgtgc tggtgcgcgt ccatacgcta agcgaaggcg tgcgcggcga tttcggcagt
     7681 atcagcattg cattaaccct gctgtgggcg ctctttacca ccatgttact catctcctgg
     7741 tatgtgattc gccggatggt tagcaacatg tatgttctgc aaagctcgtt gcagtggcag
     7801 gcgtggcacg acaccttaac ccgtttatat aatcgtggcg cactgtttga aaaagcccgt
     7861 ccgctcgcga aattgtgtca gacgcaccaa catccttttt ctgtcattca ggtcgacctt
     7921 gaccatttta aagcgattaa tgaccgcttt agtcatcagg cgggcgaccg tgttctttct
     7981 catgctgccg gattaattag cagttccttg cgtgcgcagg acgttgccgg gcgggtcggt
     8041 ggtgaggagt tttgtgtgat tctgccaggc gcgagtctga cggaggctgc ggaagtcgca
     8101 gaacgtattc gcctgaagtt aaatgaaaaa gagatgttga ttgctaagag tacgacgata
     8161 cgcatcagtg cctcgttggg ggtaagtagc agcgaggaaa ccggtgatta tgattttgaa
     8221 caactccagt cactggctga ccgtcggctt tatctcgcta aacaggccgg gcgtaatcgg
     8281 gtatgcgcga gcgataacgc ttaacgagcg gagaaaaaat ggtccagccc ttcacgccat
     8341 ccttccggtc cttcacgctg cgttcgccag acgcgggccg gatcctcatc atgcagatgc
     8401 accccttcac ggtttagccc tttcacaatc accgcgtaat ccattacctc cagtaagggc
     8461 gcatcgtttg gcccatcgcc caggccaagt gtggttgggc gtttgcctga taattgttga
     8521 taggtcgcga taatccagtt ggcggcctga tcttttccgg cagaggcatc caggacgtgc
     8581 cagaagcgcg caccctgcat aaactgtaag cccagttcgt tcagacgagc ggtaaattgt
     8641 gccatacgct cgtcactgtc gcgccagatt agcgttaccg acgcctcatg cagctgcgtc
     8701 agcgccgcct ggctacggct taatcccgtc cattcggcga tggttgcatc gtcgacatca
     8761 tcgaaagtcg tgaatttaaa atgttctttc tcgcgtagcg tatttaaaac ctggctgatt
     8821 tcgccatggc taatacctga gatgatgcgt ggaaaaccgt ctatatcctg ccattgttca
     8881 gcaagctgga tcactgcgcc attttcggca atcagtggta ccccttgtaa ccccaacgtt
     8941 ttttgcaagt acagcatttc cgctgaagtt ttactgctac agagaatgac gggaacattc
     9001 gcttcgcgta aacggctgag ccagggggca gccggttgcc agtcataact atgactgtcc
     9061 agcagggtgc catcaagatc gctaaaaacc agtagtggtt gttgaattga aaacatggtc
     9121 gcgcagttct ccccttacca ggatgattct caaaacagca caaataatca aagatgattt
     9181 tttcgggagc gatcccgttg acgccagagc caccaagggg tgacgcacgt cacatttcta
     9241 ttcataagta gcgttaatca ttcatatcgc gaatattttc ttgtcagcga aaaaaattgc
     9301 ggataaggtg atgaacacat cagatttcct ggtgtaacga attttttaag tgcttcttgc
     9361 ttaagcaagt ttcatcccga ccccctcagg gtcgggattt ttttattgtg cattcaacga
     9421 ttcacttcag agacgctgaa gtcatgaata tccagttcga aggcatccgc caattcacgc
     9481 catgtatgat attcacgtcc atcgacgcta acgtgttcag aatcgttctg gctgcgatga
     9541 acttcgcttt cgctaatttc attgatcgcc gccagcaggg catcgacatc gacgctgacc
     9601 tcacgttttg cggtatcgct gtactctttt gcggttttca tcatctttcc tcgcaaccgt
     9661 tactctgttg ttgccgccag tcgttaagtc cgcctggcat ctcttaagta tagaccgtcg
     9721 agaaaatcag cactcgcgcg cgcctggcgc tgccagaaaa acgaaattgt tctacactgg
     9781 cacaaagcca caggaggaaa acgatgaagg tgaatgatcg ggtaacagtc aaaacggatg
     9841 gcggtccgcg tcgtcctggc gtggtactag cagttgagga gtttagtgaa ggcacaatgt
     9901 acctggtttc gctggaagac tacccgctcg gcatctggtt ctttaatgaa gcagggcatc
     9961 aggacggtat ctttgtggag aaagcagagt aatcttattc agcctgactg gtgggaaacc
    10021 accagtcaga atgtgttagc gcatgttgac aaaaatacca ttagtcacat tatccgtcag
    10081 tcggacgaca tggtagataa cctgtttatt atgcgttttg atcttacgtt taatattacc
    10141 tttatgcgat gaaacggtct tggctttgat attcatttgg tcagagattt gaatggttcc
    10201 ctgacctgcc atccacattc gcaacatact cgattcggtt cggctcaatg ataacgtcgg
    10261 catatttaaa aaagaggtta tcgttgtctc ttttttcaga atatcgccaa ggatatcgtc
    10321 gagagattcc ggtttaatcg atttagaact tatcaataaa atttttctga ccaatagata
    10381 ttcatcaaaa tgaacattgg caattgccat aaaaacgata aataacgtat tgggatgttg
    10441 attaatgatg tgcttgatgt gctgactgtt agaagcatca tggatgaaac agtcctcatt
    10501 aataaacacc actgaagggc gctgtgaatc acaagctatg gcaaggtcat caacggtttc
    10561 aatgtcgttg atttctcttt ttttaacccc tctactcaac agatacccgg ttaaacctag
    10621 tcgggtgtaa ctacataaat ccataataat cgttg
//
LOCUS       DAJTMK010000137        10575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTMK010000137 DAJTMK010000000
VERSION     DAJTMK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10575
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.139"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(39..494)
                     /locus_tag="OO135_003102"
     CDS             complement(39..494)
                     /locus_tag="OO135_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5398860.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            832..2034
                     /gene="ackA"
                     /locus_tag="OO135_003103"
     CDS             832..2034
                     /gene="ackA"
                     /locus_tag="OO135_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5398861.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            2109..4253
                     /gene="pta"
                     /locus_tag="OO135_003104"
     CDS             2109..4253
                     /gene="pta"
                     /locus_tag="OO135_003104"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5398862.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYAEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPNPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            4443..5963
                     /gene="yfcC"
                     /locus_tag="OO135_003105"
     CDS             4443..5963
                     /gene="yfcC"
                     /locus_tag="OO135_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5398863.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRADFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(5996..6538)
                     /gene="yfcD"
                     /locus_tag="OO135_003106"
     CDS             complement(5996..6538)
                     /gene="yfcD"
                     /locus_tag="OO135_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5398864.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(6596..7147)
                     /gene="yfcE"
                     /locus_tag="OO135_003107"
     CDS             complement(6596..7147)
                     /gene="yfcE"
                     /locus_tag="OO135_003107"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5398865.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQKDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(7203..7847)
                     /gene="yfcF"
                     /locus_tag="OO135_003108"
     CDS             complement(7203..7847)
                     /gene="yfcF"
                     /locus_tag="OO135_003108"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5398866.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSVIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            7983..8630
                     /gene="yfcG"
                     /locus_tag="OO135_003109"
     CDS             7983..8630
                     /gene="yfcG"
                     /locus_tag="OO135_003109"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5398867.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDQGKGDQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQARLKAQLGDERSDS"
     gene            8687..9049
                     /gene="folX"
                     /locus_tag="OO135_003110"
     CDS             8687..9049
                     /gene="folX"
                     /locus_tag="OO135_003110"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5398868.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            9070..9963
                     /locus_tag="OO135_003111"
     CDS             9070..9963
                     /locus_tag="OO135_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5398869.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(10011..>10575)
                     /gene="rpnB"
                     /locus_tag="OO135_003112"
     CDS             complement(10011..>10575)
                     /gene="rpnB"
                     /locus_tag="OO135_003112"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5398870.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         2515 a         2787 c         2655 g         2618 t
ORIGIN      
        1 tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt
       61 ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg
      121 ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac
      181 cagttgagga tagcaggggg taatggcgtt acagaacgct tgcccagcca ccataatccc
      241 tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag ctgaccgccc
      301 agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa
      361 cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagcggc
      421 cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac agaacgatta
      481 tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat
      541 tcacaactca gcgggacaac attcaaaaca ttttgtcttc catacccact atcaggtatc
      601 ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat
      661 tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg
      721 caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc
      781 tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt
      841 aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca
      901 gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt
      961 atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac
     1021 agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg
     1081 cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta
     1141 gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctacac
     1201 aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac
     1261 aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc
     1321 tacgccctgc cttacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc
     1381 accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa
     1441 ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt
     1501 aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt
     1561 tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt
     1621 gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc
     1681 agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg
     1741 gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt
     1801 cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa
     1861 ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct
     1921 gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc
     1981 ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac
     2041 accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag
     2101 gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca
     2161 gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca
     2221 aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc
     2281 gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac gctgaaggtc
     2341 tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta
     2401 acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt
     2461 ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta
     2521 tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca
     2581 gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac
     2641 cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca
     2701 aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc
     2761 tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc
     2821 acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca
     2881 ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg
     2941 tgacttccgc agaccgtcct gacgtgctgg tcgccgcttg cctggcagcc atgaacggcg
     3001 tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac
     3061 tgtgcgaacg tgctttcgct accgggctgc cggtatttat ggtgaacacc aacacctggc
     3121 agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgaacgta
     3181 tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga
     3241 ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg
     3301 aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg
     3361 ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc
     3421 cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa
     3481 tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga
     3541 acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta
     3601 cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg
     3661 caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat
     3721 cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca
     3781 acccgaatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg
     3841 cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg
     3901 caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg
     3961 acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat
     4021 ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gtttatcttc ccggatctga
     4081 acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc
     4141 cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg
     4201 atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc
     4261 atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt
     4321 ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat
     4381 gaattgcgcc atttcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg
     4441 ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg
     4501 tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga
     4561 tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg
     4621 tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg
     4681 tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaaggat
     4741 taacctcagg atcgaaatac gggacagctg ttggcatcat catgtttatg ctggtgattg
     4801 gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta
     4861 ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac
     4921 ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac
     4981 cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca
     5041 cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt gctcagggga
     5101 ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca
     5161 ctatgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc
     5221 tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat gttgaacaac
     5281 gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg
     5341 tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca
     5401 ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta
     5461 ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg
     5521 tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt gatgccagcg
     5581 tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac aacgcggtcg
     5641 cggcctggtt tatgttgctc ttccaggcgg tatttaattt cttcgtgacg tccggttctg
     5701 gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc
     5761 gtcaggttac cgtgctggca ttccagtttg gtgatggctt cagtcacatc atttacccaa
     5821 cctcagcttc gttaatggca actctcggtg tgtgcagggc ggacttccgt aactggctga
     5881 aggtgggcgc gaccctactt ggactgctgt ttattatgtc cagcgtcgta gtgatcggcg
     5941 ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt
     6001 ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg
     6061 ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc
     6121 agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga
     6181 acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcggcaa
     6241 aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt
     6301 gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt
     6361 ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag
     6421 ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt
     6481 cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta
     6541 tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg
     6601 gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt
     6661 cattatccag catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg
     6721 ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggtg tgaccgtaca
     6781 ccagcacatc cttctggttt aaagcaggta gattttccgg gccaaaaaga tgaccatgcg
     6841 tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat
     6901 gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg
     6961 ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac
     7021 gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa
     7081 acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca
     7141 gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc
     7201 tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac
     7261 tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg
     7321 ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga
     7381 cccagtgcta acaaatgttc tgccatcgcg aacagtttct ctgcactggc ttttccctcg
     7441 gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg
     7501 atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct
     7561 aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc
     7621 gcaatgacag aagattcact caactcaaaa tcatcgattt gtaataacgg cacacgacgt
     7681 gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc
     7741 ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aagcggataa cacataaggg
     7801 gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca
     7861 ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact
     7921 cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg
     7981 caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg
     8041 aagaagcaga gcttgattat cgcttgatta aggtagacca ggggaaaggc gatcagtttc
     8101 gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc
     8161 ctgccgatgg cggcgaaccg ctaagcctct ttgaatctgg tgccattttg ttgtatctgg
     8221 ctgagaaaac ggggctcttt ttgagtcatg aaacgcgtga acgcgccgcc acattacagt
     8281 ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc
     8341 acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc
     8401 tttaccatgt actaaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca
     8461 gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc
     8521 tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg
     8581 ggcaggcacg gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg
     8641 ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc
     8701 cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta aggaagaaga
     8761 aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc
     8821 gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat
     8881 tcagcatgta gagaataacc gtttctcttt gttggaaaaa ttaactcagg atgtgctcga
     8941 tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc
     9001 gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg
     9061 ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt
     9121 ccgcgtttgc tggagctggg ccatcaaatc acggtagtga cgcgtaaccc acagaaagcc
     9181 agttccgttc tcggccctcg ggtgacgcta tggcaagggc tagccgatca aagcaacctc
     9241 aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact
     9301 cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat
     9361 ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat
     9421 tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact
     9481 cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga
     9541 gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg
     9601 ctgccaccgt ttcgtcttgg cctgggcggg ccgattgact ctggtcggca gtatctggcc
     9661 tggattcata ttgatgatat ggtcaacggt attctctggc tgctggataa cgagctgcgc
     9721 gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc
     9781 ggtcacgcgc tgcatcgccc ggccattttg cgcgtccccg cgaccgccat tcgactgtta
     9841 atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa
     9901 gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc
     9961 tgatgtggtt tacagcaaac atccgccagt taactcccgg tgttacagga ttagtggctt
    10021 tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa
    10081 cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc ctgcattgcg
    10141 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    10201 tccgctatct caccaataaa tgcacgaaaa cgctgggcat cccctgtttg taatacgtaa
    10261 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt
    10321 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    10381 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    10441 aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc aagccagcag
    10501 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    10561 agctctttat agccc
//
LOCUS       DAJTMK010000138        10543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTMK010000138 DAJTMK010000000
VERSION     DAJTMK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.140"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..449
                     /locus_tag="OO135_003113"
     CDS             <1..449
                     /locus_tag="OO135_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5398871.1"
                     /translation="LNKFTGGISQLDDAAEQKCILESIQQLKVKTSSPDLAIGRLSGG
                     NQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQLVQQGIAVIVISSELPE
                     VLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHVEKQSV"
     gene            427..1608
                     /gene="xylH"
                     /locus_tag="OO135_003114"
     CDS             427..1608
                     /gene="xylH"
                     /locus_tag="OO135_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5398872.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            1686..2864
                     /gene="xylR"
                     /locus_tag="OO135_003115"
     CDS             1686..2864
                     /gene="xylR"
                     /locus_tag="OO135_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR5398873.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(2972..3796)
                     /locus_tag="OO135_003116"
     CDS             complement(2972..3796)
                     /locus_tag="OO135_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5398874.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            4116..6146
                     /gene="malS"
                     /locus_tag="OO135_003117"
     CDS             4116..6146
                     /gene="malS"
                     /locus_tag="OO135_003117"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5398875.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSTPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLIDSAHQRGIRILFDVVMNHTGYATLVDMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPEKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVVHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            6324..7577
                     /gene="avtA"
                     /locus_tag="OO135_003118"
     CDS             6324..7577
                     /gene="avtA"
                     /locus_tag="OO135_003118"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5398876.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(7728..8201)
                     /locus_tag="OO135_003119"
     CDS             complement(7728..8201)
                     /locus_tag="OO135_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5398877.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPPCVEACPTKALQFMDVEKVQRHRLRQQPV"
     gene            complement(8303..9151)
                     /gene="yiaJ"
                     /locus_tag="OO135_003120"
     CDS             complement(8303..9151)
                     /gene="yiaJ"
                     /locus_tag="OO135_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5398878.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIMH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            9352..10344
                     /gene="yiaK"
                     /locus_tag="OO135_003121"
     CDS             9352..10344
                     /gene="yiaK"
                     /locus_tag="OO135_003121"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5398879.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHCIGLVALRNANHWMRGGSYGWQTAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            10356..>10543
                     /locus_tag="OO135_003122"
     CDS             10356..>10543
                     /locus_tag="OO135_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5398880.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQIIDLTTREAVENRP"
BASE COUNT         2605 a         2700 c         2646 g         2592 t
ORIGIN      
        1 cactcaataa atttaccggt ggcattagcc agcttgatga cgcggcagag caaaaatgta
       61 ttctggaatc aatccagcaa ctcaaagtta aaacgtcgtc ccccgatctt gctattggac
      121 gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg ctgtctgtta ctcaacccgc
      181 gcattctcat tcttgatgaa cctaccaggg gtattgatat tggcgcgaaa tatgagatct
      241 acaaattaat taaccaactc gtccagcagg gtattgccgt tattgtcatc tcttccgaat
      301 tacctgaagt gctcggcctt agcgatcgtg tactggtgat gcatgaaggg aaactaaaag
      361 ccaacctgat aaatcataac ctgactcagg agcaggtgat ggaagccgca ttgaggagcg
      421 aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg ccgtaccgac atccggtggc
      481 ttttccgggc tgaaatcact gaatttgcag gtcttcgtga tgattgcagc tatcatcgca
      541 atcatgctgt tctttacctg gaccaccgat ggtgcctact taagcgcccg taacgtctcc
      601 aacctgttac gccagaccgc gattaccggc atcctcgcgg taggaatggt gttcgtcata
      661 atttctgctg aaatcgacct ttccgtcggc tcaatgatgg ggctattagg tggcgtcgcg
      721 gcgatttgtg acgtctggtt aggctggcct ttgccactta ccatcattgt gacgttggtt
      781 ctgggactgc ttctcggtgc gtggaacgga tggtgggtcg cgtaccgtaa agtcccttca
      841 tttattgtca ccctcgcggg catgttggca tttcgcggca tactcattgg catcaccaac
      901 ggcacgactg tatcccccac cagcgccgcg atgtcacaaa ttgggcaaag ctatctcccc
      961 gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg cttttgttgg ttggcaatgg
     1021 cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc cggcctctac cgcagtagtc
     1081 ggtcgccagg ctttaaccgc tatcatcgta ttaggcgcaa tctggctgtt gaatgattac
     1141 cgtggcgttc ccactcctgt tctgctgctg acgttgctgt tactcggcgg aatgtttatg
     1201 gcaacgcgga cggcatttgg acgacgcatt tatgccatcg gcggcaatct ggaagcagca
     1261 cgtctctccg ggattaacgt tgaacgcacc aaacttgccg tgttcgcgat taacggatta
     1321 atggtagcca tcgccggatt aatccttagt tctcgacttg gcgctggttc accttctgcg
     1381 ggaaatatcg ccgaactgga cgctattgca gcatgcgtga ttggcggcac cagcctggct
     1441 ggcggtgtgg ggagcgttgc cggagcagta atgggggcat ttatcatggc ttcactggat
     1501 aacggcatga gtatgatgga tgtaccgacc ttctggcagt atatcgttaa aggtgcgatt
     1561 ctgttgctgg cagtatggat ggactccgca accaaacgcc gttcttgatt ttgataaaaa
     1621 ttttctcaaa gccggttacg tattaccggt tttgagtttt tgcatgattc agcaggaaaa
     1681 gaaccatgtt tactaaacgt caccgcatca cattactgtt caatgccaat aaagcctatg
     1741 accggcaggt agtagaaggc gtaggggaat atttacaggc gtcacaatca gaatgggata
     1801 ttttcattga agaagatttc cgcgcccgca ttgataaaat caaggactgg ttaggagatg
     1861 gcgtcattgc cgacttcgac gacaaacaaa tcgagcaagc gctggctgat gtcgacgtcc
     1921 ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag ttacccaccc gttcattaca
     1981 ttgccaccga taactatgcg ctggttgaaa gcgcattttt gcatttaaaa gagaaaggcg
     2041 ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg caaacgttgg gccactgaac
     2101 gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta tcgcggagtg gtttatcagg
     2161 ggttagaaac cgcgccagag aactggcaac acgcgcaaaa tcggctggca gactggctac
     2221 aaacgctgcc accgcaaacc gggattattg ccgttactga cgcccgggcg cggcatattc
     2281 tgcaagtatg tgaacatcta cacattcccg taccggaaaa attatgcgtg attggcatcg
     2341 acaacgaaga actgacccgc tatctgtcgc gtgtcgccct ttcttcggtc gctcagggcg
     2401 cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg attattagat aaagaagaaa
     2461 tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat tgaacggcgc tcaacagatt
     2521 atcgctcgct gaccgatccc gccgttattc aggccatgca ttacattcgt aatcacgcct
     2581 gtaaagggat taaagtggat caggtactgg atgcggtcgg gatctcgcgc tccaatcttg
     2641 agaagcgttt taaagaagag gtgggtgaaa ccatccatgc catgattcat gccgagaagc
     2701 tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc gatcaatgag atatcgcaaa
     2761 tgtgcggtta tccatcgctg caatatttct actctgtttt taaaaaagca tatgacacaa
     2821 cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt gtagcgatga tgagaattgt
     2881 cggcgtcaca tcagggaact cagcacagcc acctgatgcg agcaaaagcc ttatccgacc
     2941 gacaaatcag ataaggctca aggaaatgca attacatatg cgccgcgatt aaccgttggt
     3001 tatcctggta cattgcgaac aggtagttat tataactctt ccctttggtc gagtagccct
     3061 tcagcttgtg aatcatcgct gtggcagtca cttcctgatc cgctttacgc agctgcgcac
     3121 gcgatttacg gaacgaagag taagccgggt gcgtattcag gttagtgaca taggcgctca
     3181 ccgattcttt gacagaacta aactgtgagt accctttcac tttacccggc gcattggtac
     3241 aacgtccttt catgcatttc atgccgaaca gattgttatt gttgcgcgcc agcttcgacg
     3301 ttccccaacc gctttctgct gcagccatcg tcgccaccat actggtaggg ataatgtcta
     3361 cgcgttcaag caaggtattc cacgggattt ttcgcgtatt accagaccac ttcaccttgt
     3421 agcgtttggc gatgtctttc agacgcgcac gctcagcagg tgaccattgg ccctgatact
     3481 gttttgaaat cagccagtta cgttctgcag taatggctgc attttggctg gtaatgtaag
     3541 gcattacggt ccggagaaac gcctttttcc ttggtgttcc ggaagggtat tttcgcaaat
     3601 caggaagtga actactcttt gcactattgc gagaatactc ttgtttactg cttacctgtt
     3661 tattactagc tttagttaag tgggactttt gactcgctgt tgttgtgtgc gtcttcgcta
     3721 acacctcact cgaaaacacc agagtgagta acataagaat catcgcccca tatcgtcgta
     3781 tgggagtcaa aatcatcagg tctcctggtc ggatttaatc attccaacac cttatatttt
     3841 tcacaaattt gagagttgaa tctcaaatca tatcaaaaat agctgtcaag agcaccccaa
     3901 ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat tcagattggc gctaaatcgc
     3961 agaaaatgtg gggtttatcg caaaattcag ccgttttttg cgcgcgatcg ctcacccttg
     4021 cttctcatcc tgtggactta ccgctcaggg atgagttttg tctggcttat cgctggcaaa
     4081 ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa actcgcctcc tgttttctga
     4141 cactccttcc tggcttcgcc gttgccgcca gctggacttc tccggggttc cctgccttta
     4201 gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt gcccaaaggt acgcgtccac
     4261 tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga tgcgataaaa ctcaatcaga
     4321 tgctttccct gcaaccttgt agcaacacgc cgcctcaatg gcgattgttc agggacggca
     4381 aatatacgct gcaaatagac acccgctccg gtacgccaac attgatgatt tccatccaga
     4441 acgccgccga accggtagca aacctggtcc gtgaatgccc gaaatgggat ggattaccgc
     4501 tcacgctgga tgtcagcgcc actttcccgg aaggagccgc cgtacgggat tattacagcc
     4561 agcaaattgc gatagtgaag aacggtcaaa taacgttaca acccgctgct accagcaacg
     4621 gtttactcct gctggaacgg gcagaaactg acacatccac ccctttcgac tggcataacg
     4681 ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg cgatcccagt aatgaccaga
     4741 gttacggacg tcataaagac ggtatggcgg aaattggcac ttttcacggc ggcgatttac
     4801 gcggcctgat caacaaactg gattacctcc agcagttggg cgttaatgct ttatggataa
     4861 gcgccccatt tgagcaaatt cacggctggg tcggcggcgg tacaaaaggc gatttcccgc
     4921 attatgccta ccacggttat tacacacagg actggacgaa tcttgatgcc aatatgggca
     4981 acgaagccga tctacggacg ctgattgata gcgcacatca gcgcggtatt cgtattctct
     5041 ttgatgtagt gatgaaccac accggctatg ccacgctggt ggatatgcag gagtatcagt
     5101 ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac gctgggtgaa cgctggagcg
     5161 actggaaacc tgccgccggg caaacctggc atagctttaa cgattacatt aatttcagcg
     5221 acaaaacagg ctgggataaa tggtggggaa aaaactggat cagaacggat atcggcgatt
     5281 acgacaatcc tggattcgac gatctcacta tgtcgctggc ctttttgccg gatatcaaaa
     5341 ccgaatcaac taccgcttct ggtctgccgg tgttctataa aaacaaaacg gatactcacg
     5401 ctaaagtcat cgaaggcttt acacctcgcg attacttaac ccactggtta agtcagtggg
     5461 tccgcgacta tgggattgat ggttttcggg tcgataccgc caaacatgtt gagttgcccg
     5521 cctggcagca actgaaaacc gaagccagct ccgcgcttcg cgaatggaaa aaaactaacc
     5581 ccgagaaagc attagatgac aaacctttct ggatgaccgg tgaagcctgg ggccacggcg
     5641 tgatgcaaag tgactactat cgccacggct tcgatgcgat gatcaatttc gattatcagg
     5701 agcaggcggc gaaagcagtc gattgtctgg cgcagatgga tacgacctgg cagcaaatgg
     5761 cggagaaatt gcagggtttc aacgtgttga gctacctctc gtcgcatgat acccgtctgt
     5821 tccgtgaagg gggcgacaaa gcagcagagt tattactatt agcgccaggc gcggtacaaa
     5881 tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac aggttctgat ccgctgcaag
     5941 gtacacgttc ggatatgaac tggcaggatg ttagcggtaa atctgccgcc aacgtcgtgc
     6001 actggcagaa aatcagccag ttccgcgccc gccatcccgc aattggcgcg ggcaaacaaa
     6061 cgacactttc gctgaagcag ggctacggct ttgttcgtga gcatggcgac gataaagtgc
     6121 tggtcatctg ggctgggcaa cagtgacttt tccggcttcc cgttcatcag tacctcggga
     6181 agccgccaac caggataaaa tgtcagccct aatcagcgtt gcaggataaa gcaccgctca
     6241 ctcttcaaca gaccgatttg caccccagca aatgtagcgt tattgttacc ttccttgcta
     6301 cagagttcga cagatatccc gctatgacat tctccctttt tggtgacaaa tttacccgcc
     6361 actccggcat tacgctgctg atggaagatc tgaacgacgg tttacgcacg cctggcgcga
     6421 ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat gcaggactac ttccagacgc
     6481 tactgaccga catgctggaa agtggcaaag cgactgatgc actgtgtaac tacgacggtc
     6541 cacaggggaa aacggagcta ctcacactgc ttgccggaat gctgcgcgag aagttgggtt
     6601 gggatatcga accacagaat attgcactaa caaacggcag ccagagcgcg tttttctact
     6661 tatttaacct gtttgccgga cgccgtgccg atggtcgggt caaaaaagtg ctgttcccgc
     6721 tggcaccgga atacattggc tacgctgacg ccggactgga agaagatctg tttgtctctg
     6781 cgcgtccgaa tattgaactg ctgccggaag gccagtttaa ataccacgtc gattttgagc
     6841 atctgcatat tggcgaagaa accgggatga tttgcgtctc ccggccgacg aatccaacag
     6901 gcaatgtgat tactgacgaa gagttgctga agcttgacgc gctggcgaat caacacggca
     6961 ttccgctggt aattgataac gcttatggcg tcccgttccc gggtatcatc ttcagtgaag
     7021 cgcgcccgct atggaatccg aatatcgtgc tgtgcatgag tctttccaag ctggggctgc
     7081 ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat catcactgcc atcaccaata
     7141 tgaacggcat tatcagcctg gcacctggcg gtattggtcc ggcgatgatg tgtgaaatga
     7201 ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat caaaccgttt tactaccagc
     7261 gtgttcagga aactatcgcc atcattcgcc gctatttacc ggaaaatcgc tgcctgattc
     7321 ataaaccgga aggagccatt ttcctctggc tatggtttaa ggatttgccc attacgaccg
     7381 agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat ggtgccgggg cacaacttct
     7441 tcccgggact ggataaaccg tggccgcata cgcatcaatg tatgcgcatg aactacgtac
     7501 cagagccgga gaaaattgag gcgggggtga agattctggc ggaagagata gaaagagcct
     7561 gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt atcaggccta catgatttct
     7621 gcaatatatt gaatttggaa gaatttgtag gccggataag gcgtttacgc cgcatctggc
     7681 ataaacaaag cgcactattc cggcgttgag aaacgccgga aaacgtttca aacaggctgc
     7741 tgccgtagcc gatgccgctg cactttctcg acatccatga actgcaacgc ctttgtcggg
     7801 caggcttcaa cacagggcgg ccccgtctcc cgatgccagc acagatcgca cttaatcgcc
     7861 ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc cagcatacag
     7921 cttttacagc caatgcaacg tgtttgttca acgaaaatat gcccatgttc gcggcttatc
     7981 gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac actgatgaca ggctactgcc
     8041 gtggtccagc tgtggtcttt aatgacacga atacgggaaa taaactcgtc tggtgacaac
     8101 gcagcgcaat cctgattctc ctgatgcgac actgcgcaag ccacttcaca ggtacggcaa
     8161 ccgatacatt tcgtcgcatc cgcaataata aaccggttca tgatcactcc tttgcactaa
     8221 aacagcagag tgccagagag cgcgtcccta ctgctttgat ccaacgaagg aaatgttaaa
     8281 gttttggcac ttgtccaaag cgttatgtga tcgcgcccag gtcatccctg acagtaaatc
     8341 ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga tttttctctc
     8401 ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc acccgcccat
     8461 gaatatcaaa caccggaaca gcaatacagg agacgccgag ttcgttttct tctctgtcca
     8521 tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc agctcggtaa
     8581 tggtattgcg ggttaacggc tggatctcat gctgatggtt ttcccagtat gacttcacgt
     8641 agtccgggtg accaaacgcc atgtagatct tgcccattgc ggaacagtag agcggcatat
     8701 gctggccaat ataggcacgg gttcgcagca tcccggttgt gggttccagc ttataaatca
     8761 aaatagcgtg atcgtcttcg cggctggaga agttaatggt ttcaccagtg gcgatgttca
     8821 gtgcctcaag atgcggagcg gcgatatgca tgatattcag cgaagacagc gccttctgcc
     8881 cgacggcaat aaatttggta gtcaggcggt aactccctgc ggcaggcgcg gtggtcacat
     8941 agccgcagga ctgtaatccc tgcaataagc gatggacggt actcttattt aaaccagcca
     9001 gctccgaaag atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc tcaatcagca
     9061 tcaacccgcg aaacagactc tggcttccgg ctggacgctc tttttcctgc gccatctcgt
     9121 tctctttttt tcccatcact tctttcccca ttttgtcgcg tcctgatggt agcgcaaagt
     9181 gtgccgtagt tcacgatctc gacagataat ttataaccac ttgattttta tgtcttttga
     9241 aattcatcaa tcagatttct ttgttaaaaa gtgatcgata tatttgaaat caagtttcgc
     9301 ttattgaaat tttaagccaa aaaagcgatc aaaaaaacaa ggaagcctgg gatgaaagtg
     9361 acatttgagc agttaaaagc agcctttaat cgggtcttaa tttcacgcgg cgttgacagc
     9421 gaaacggctg acgcctgtgc agagatgttc gcccgcacca ccgaatccgg cgtttattct
     9481 cacggcgtta atcgtttccc tcgtttcatt caacaactgg aaaacggcga tatcattcct
     9541 gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg aacagtggga tgcccagcgt
     9601 tcgatcggca acctgacggc gaaaaagatg atggatcgcg ccattgaact ggctgccgat
     9661 cactgtattg gtctggtggc acttcgtaat gccaaccact ggatgcgcgg cggcagctac
     9721 ggctggcaga cggcggaaaa aggctatatt ggcatttgct ggaccaactc catcgccgta
     9781 atgccgccgt ggggcgcaaa agagtgtcgc attggcacta acccgctgat cgtcgccatt
     9841 ccttccacgc cgatcaccat ggtcgatatg tcgatgttct cttacggcat gttagaagtt
     9901 aaccgcctgg cgggccgtca gctcccggtc gatggtggct ttgatgatga gggcaatttg
     9961 accaaagaac ctggcgttat cgagaagaat cgccgcattt tgccgatggg ctactggaaa
    10021 ggttctggca tgtcgattgt gctggatatg atcgctactc tcctttccga cggcgcatcg
    10081 gttgccgaag tcacccagga caacagcgac gaatacggca tttcacaaat ttttattgcc
    10141 attgaagtgg acaagcttat cgacggtccc acccgcgatg ccaagctgca acgcatcatg
    10201 gattacgtta ctaccgctga acgcgctgac gaaaaccagg ccatccgctt acccggccac
    10261 gaatttacta ccctgctggc cgaaaaccgc cgtaacggca tcactgttga tgacagcgtg
    10321 tgggccaaaa tccaggcgtt atgaggagat aagtcatgat ttttggacat atcgcgcagc
    10381 ccaatccgtg ccgtttgccc gccgccattg aaaaggcgct cgattttctg cgcgctacca
    10441 acttcaacgt cctggagccg ggcgttgtcg aaatcgacgg caagaatatt tatgcgcaaa
    10501 ttatcgattt aaccacacgc gaagcggtgg aaaaccgtcc gga
//
LOCUS       DAJTMK010000139        10351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTMK010000139 DAJTMK010000000
VERSION     DAJTMK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.141"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..352
                     /locus_tag="OO135_003123"
     CDS             <1..352
                     /locus_tag="OO135_003123"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398881.1"
                     /translation="ILNSLSRSPEESIPVSCRTWSETKAAYCCFSNDKVSANKIMAPH
                     KENITERAQQFKRVLVLQDTTELNYSGQKEKQGVGHKNIKMKETYFFILNSSSVSQVF
                     ASVFMMTISGSGMN"
     gene            complement(802..1995)
                     /locus_tag="OO135_003124"
     CDS             complement(802..1995)
                     /locus_tag="OO135_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5398882.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2131..3855
                     /gene="iucA"
                     /locus_tag="OO135_003125"
     CDS             2131..3855
                     /gene="iucA"
                     /locus_tag="OO135_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5398883.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3856..4803
                     /gene="iucB"
                     /locus_tag="OO135_003126"
     CDS             3856..4803
                     /gene="iucB"
                     /locus_tag="OO135_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5398884.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEHEIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4803..6545
                     /gene="iucC"
                     /locus_tag="OO135_003127"
     CDS             4803..6545
                     /gene="iucC"
                     /locus_tag="OO135_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5398885.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTNEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6542..7879
                     /gene="iucD"
                     /locus_tag="OO135_003128"
     CDS             6542..7879
                     /gene="iucD"
                     /locus_tag="OO135_003128"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5398886.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7885..10080
                     /gene="iutA"
                     /locus_tag="OO135_003129"
     CDS             7885..10080
                     /gene="iutA"
                     /locus_tag="OO135_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5398887.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2539 a         2638 c         2711 g         2463 t
ORIGIN      
        1 tatcctcaat agcctcagtc gttcaccaga agaaagtatt cctgtttcct gccgtacctg
       61 gagtgaaaca aaggcagctt attgttgttt ttcaaacgat aaagtctctg ctaataaaat
      121 tatggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      181 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtca
      241 caaaaacata aagatgaaag aaacttattt cttcatcctc aactcgtcat cagtgagtca
      301 ggtgtttgcc tcggtgttta tgatgactat cagtggttca gggatgaatt aaaaaccagt
      361 aaaaataccc gacaagaaat ctgcaatgat ttacttcata aaaaacatgt cagtgaaaaa
      421 gaaacctggc gttgggttga aggatataat aaagcaacag aactggctcg ccaatgccct
      481 gatacccatg tattgtcaat ttctgatcgt gaaggcgatt tttatgacct gtttgaacgc
      541 gcagagcaga ctcccggaat aaaagctgac tggcttgtca gaatgaaatt taataacaga
      601 gcaacgctga atattaatgg taaacgggat cataggttgt tgcacgaaag aatcatggag
      661 attacaccgc agcaactggt tgaattcact atacctgatg ggcgggggca gcaagccaga
      721 acactgcctg acatgctttc atcagacgca ggtcctctct catatcctct gatttactcc
      781 agtaaggtga tgatcatatc tttatttgat agtacggtaa tgccagataa atacagacac
      841 aatggtaaat aatatagttg caaataatgc tgccataatc agcgcgattg cgacacctgt
      901 tttcgatacc agcggcacca gcaaagcgca cagtccatag ccaagtgtgt gactggtagc
      961 gagccagccc gcgcctgcgc catcagccag cttatcattc agtagaagtt gataagcggg
     1021 tgttgccaga gctgctccaa atgacagcac tgcacagccc gggtaaaaca accatagctg
     1081 ttcggagagc atgatagcca gaccaccact catcaacact ccggcactca ggagcagggc
     1141 caccggagtc aggcgctgcg gacggactac cccgaactgc gcgataagcg cagctactgc
     1201 ggacagaccc aacagccagg ccacctgttg gctaatggcg gtggtatccg tgacgaactg
     1261 gcgagtaagg gcaggcgaaa gtccaagctg catcatgctc accgccgcag ccagtaatat
     1321 tgcgcaaagc agataaggca gacaatcccg cttcagactg acgctcttgc actccggtgt
     1381 gggctgtggt ggtgtgccgg gcaaccgcag cagcatcagc agcgccagca ctggtgctgc
     1441 catcagtagc cccagtggcg ccagaggatg aatggccagc attgctgccg cgcacagcgg
     1501 cccgaatagc ctgccgcaac tcaggccgga gctgatggtt gccagagcgg ccatgcgatt
     1561 cccttctcca gctctctgca atgcccagac ctgacaggct ggcaccattg ccgacacggt
     1621 cagcccgtag gcgatccggg cgatgattaa tattcccagt cccaccgctg ttgttatggc
     1681 gctggtagcc atcagcacgc ttcccagccc tagcagggta aagcttatgc catatcctcc
     1741 cagagcccac aacaccacag gtttacttcc agtacgggaa atttgctttc cccaccacgg
     1801 ggacgatggc agaaacagca ttgagccaag catcagcagg gcagcccata cagacagact
     1861 cagatttgtc tgtatgacca gaacagggag cacaaccagc agaccgttct gtccgatacc
     1921 gagaagcccg gcactgaacg caagtggcca gcaggacagt gttttttggg gcgtatcttc
     1981 gatccctggt gacattataa tgtttcaact ccatgtatta attgtgttta tttgtaaaat
     2041 taatttatct gacaataaca tttattattg ataatgagaa tcattatcga cataattgtt
     2101 attattttac tgtgtgggag ctgtttgact atgaccctgc cctctgaaaa accagccaca
     2161 gatgtggctg cgcagtgctt cctgaatgca ctgattcgtg aaaccacaga ctggaaactg
     2221 acagaatacc cgccagacga attgctcatc ccgctggatg agcagaaatc gctccatttc
     2281 agagtggctt atttctcccc aacccagcat caccgctttg catttcctgc acgtctggtc
     2341 acggcatcag gcagttatcc tgtcgacttt accactctct cccggcttat tattgataag
     2401 ctacgccatc aactgtttct gcccgttccc ctctgcgaaa ctttccacca gcgcgtgctg
     2461 gaaagccatg tccatacgca acaggcaatt gatgcccgtc atgactgggc cgctctgcgt
     2521 gaaaaagcgt tgaattttgg cgaggctgag caggcgctgc tgacaggaca cgctttccac
     2581 cctgcgccta agtctcatga accgtttaac cggcgggagg ctgaacgcta cctgcctgat
     2641 atggcacccc acttcccact gcggtggttt tcggtggata aaacgcaaat cgccggtgaa
     2701 agtttgcatc tcaaccttca acagcggctg acgcgatttg ccgcagagaa tgcgcctcag
     2761 ttactcaacg aattaagtga caaccaatgg ctgttcccgt tgcacccgtg gcagggggaa
     2821 tatcttttgc agcaggggtg gtgccaggca cttgttgcta aagggctgat taaagactta
     2881 ggtgaggccg gcacgtcgtg gctgccgacc acctcttccc gttccctcta ctgtgccacc
     2941 agccgcgata tgatcaagtt ctccctgagc gtacggctga ccaactccat ccgtactctg
     3001 tccgtgaaag aagtgaagcg tgggatgcgc ctggcacgcc tggctcaaac cgacggctgg
     3061 cagatgctac aggtccgctt cccgactttc cgggtaatgc aggaggatgg ctgggccggg
     3121 ctgctcgatc ttaacggcaa catcatgcag gaaagtctgt ttgccctgcg tgaaaatctg
     3181 ctggtggatc agccgaaaag ccagactaac gtactggtct ccctgactca ggccgcacct
     3241 gatggcggtg attcgctgct ggtttcggcg gtaaaacgcc tgagcgatcg cctcggtatc
     3301 actgtgcaac aggccgccca tgcatgggtc gatgcatact gtcagcaagt gctaaagccg
     3361 ctgtttacgg ctgaagcgga ttacggcctg gtgctgctgg cgcatcagca aaatattctt
     3421 gtccagatgc ttggggatct gccggtcgga tttatttacc gtgactgtca gggcagcgct
     3481 tttatgcctc acgcgacaga ctggctcgat tctattggcg aggcgcaggc ggaaaatatt
     3541 ttcacccatg agcagttgct gcgctatttc ccttattacc tgctggttaa ctccactttt
     3601 gctgtgaccg ctgcgctggg ggctgccggg ctggacagcg aatcgaatct gatggctcgt
     3661 gtacgagcat cgctggctga agtgcgtgat caggtgactc ataaaacctg tctcaactac
     3721 gtgctggaaa gtccgtactg gaacgtaaaa ggtaactttt tctgttatct gaacgatcat
     3781 aacgagaaca ccattgttga cccttcggtg atctatttcg atttcgctaa cccgctgcag
     3841 gctcaggagg tctgaatgtc tgaggcaaac attattcaca gcagatatgg actgcgctgt
     3901 gaaaaactcg acaagcccct gaatcttggc tggggactgg acaatagcgc ggtgttgcac
     3961 tgtcccgggg agctgccgac agggtggctg tgcgatgcgc tggatcagat atttatcgcc
     4021 gcaccacaac tgtcagcagt ggctctcccc tgggccgaat ggcgtgagga gccacaggcg
     4081 ctgacgcttt ttgggcaggt aaaaagcgac attatccatc gtactgcctt ctggcagtta
     4141 ccgttatggt tgagttcacc ggcaaaccgg gcctccggtg aaatggtttt tgatgcagag
     4201 catgagattt atttcccgca gcgcccaccc cgtccacagg gtgaggttta ccgccgttac
     4261 gatccacgta ttcgcaggat gctgagtttc cgcattgccg atcccgtttc tgatgcagaa
     4321 cgtttcaccc gctggatgaa tgatccgcgc gttgagtatt tctgggagca aagtggctca
     4381 ctggaggtac agaccgccta tctggagcgc cagctcaccg gtaaacatgc attcccgctg
     4441 attgggtgct tcgacgatcg gccattcagc tatttcgaaa tctactgggc ggcggaagac
     4501 cgcattggtc gtcactattc gtggcagccc tttgaccgtg gcctgcatct gctggttggt
     4561 gaacagcaat ggcgcggggc ccactatgtg caaagctggc tgcgcgggct gacacattac
     4621 ctgctgctgg atgagccccg tacgcagcgc acagtactgg agccacgcac cgataaccag
     4681 cgcctgttcc gccatcttga gcctgcggga taccggacaa ttaaagagtt cgacttccca
     4741 cataagcgct cgcgcatggt gatggcggat cgtcatcact tcttcacgga ggtcggtctg
     4801 taatgaatca caaggactgg gattttgtta accgtcggct ggtggcaaaa atgttgtctg
     4861 agatggagta tgagcaggtt ttccacgcgg aatctcaggg cgatgaccac tactgcatta
     4921 acctgccggg agcacaatgg cgcttcatcg ctgaacgtgg tatctggggc tggctgtgga
     4981 ttgatgccca aacgttgcgc tgcacgaacg aaccagtact ggcgcagacg ctgctgatgc
     5041 agctaaagcc ggtgctgtca atgagtgatg ccactgttgc agagcatatg caggatttgt
     5101 atgccacgct actgggcgac ctgcaactgc tgaaggcccg tcgcggactg agtgccagtg
     5161 acctgattaa tcttgatgcc gatcgtctgc aatgcctgct gagcggtcat cctaaattcg
     5221 tttttaataa aggtcgccgt ggctggggca aagaggcgct ggaacgatat gcgccagagt
     5281 ataccaacac cttcagactg cactggctgg cggtaaaacg tgaacatatg atctggcgct
     5341 gcgataacga tctggatatt cagcagttgt tgactgccgc aatggatccg caggagttta
     5401 cacgcttcag tcaggtctgg caggaaaacg ggctggatca caactggcta ccgctgccgg
     5461 tacatccgtg gcagtggcag caaaaaatcg ccacagactt catcgctgat tttgtcgaag
     5521 gcaggatggt gtctctcggc gagtttggcg accagtggct cgcccagcag tcgctgcgta
     5581 ccctgaccaa cgccagccgc cggggagggc tggatatcaa gctgccgctg accatctaca
     5641 acacctcatg ctaccggggg atccctggca gatatatcgc tgccggacca ctggcttcac
     5701 gctggctgca acaggttttt gcgaccgacg ccaccctggt gcaaagcggc gcagtgatcc
     5761 ttggtgaacc ggcggcaggc tatgtgtccc atgaaggcta tgccgcgctt gcccgggctc
     5821 cctatcgcta ccaggaaatg cttggtgtta tctggcggga gaatccgtgc cgctggctga
     5881 aaccggatga aagtcctgtt ctgatggcaa cactgatgga gtgcgacgaa aacaatcagc
     5941 cgctggcagg cgcatatatc gaccgctccg gactggacgc tgaaacctgg cttacgcaat
     6001 tgttccgggt ggttgtggtt ccgctgtatc acctgctttg ccgctacggt gtcgcgctta
     6061 ttgcacacgg acaaaatata acgctcgcca tgaaagaggg ggtaccacag cgtgttctgc
     6121 tgaaagactt ccagggcgat atgcggctgg tgaaagaagc gtttcccgaa atggactctt
     6181 tgcctcagga ggtgcgtgat gccacatccc gtctgagtgc ggactactta atccatgatt
     6241 tgcagacggg tcacttcgtg acagtactgc gttttatttc gccactgatg gttcgtcttg
     6301 gcgtacctga aaggcgattt tatcaactgc tggcagcagt gttgagtgat tacatgaaca
     6361 aacatccaca aatggcagag cgttttgcgc ttttctcact cttcaggcca caaatcattc
     6421 gcgtggtgct gaacccggta aaactgacct ggccggacct ggatggcggc agtcgcatgc
     6481 tgccgaatta ccttgagaat ctgcaaaatc cgctgtggct ggtaactcag gaatatgaat
     6541 catgaaaaaa agtgtcgatt ttattggtgt cgggacaggg ccatttaatc tcagcattgc
     6601 tgcattgtca catcagatcg aagaactgaa ctgtctcttc tttgacgaac atcctcattt
     6661 ttcctggcat ccgggtatgc tggtaccgga ttgtcatatg cagaccgtct ttctgaaaga
     6721 tctggtcagt gcagttgcac ctacaaatcc ctacagcttt gttaactatc tggtgaagca
     6781 caaaaagttc tatcgcttcc ttacaagcag gctacgtaca gtatcccgtg aagagttttc
     6841 tgactatctc cgctgggctg ctgaagatat gaataacctg tatttcagtc ataccgttga
     6901 aaatattgat ttcgacaaaa aaagtcgatt gtttctggtt cagaccagtc ggggagaata
     6961 ttttgcccgc aatatctgcc ttggtacagg aaaacaacct tatttaccac cctgtgtgaa
     7021 gcatgtgaca caatcctgtt tccatgccag tgaaatgaat cttcgtcggc cggacctgag
     7081 tggaaaacgg ataaccgtgg ttggtggagg acagagtggt gcagacctgt tccttaatgc
     7141 attacgcggg gaatggggag aagcggcgga aataaactgg gtctcacggc gtaataattt
     7201 taacgcactg gatgaggctg cttttgctga tgagtatttt acacctgaat atatttcagg
     7261 cttctccgga ctgaaggaag atattcgcca tcagttactg gatgagcaga aaatgacatc
     7321 ggatggcatc actgccgatt ctttactgac catttatcgt gagttgtacc accgttttga
     7381 agttctgaga aaaccaagaa atatccgtct gctacccagt cgctcggtaa caactctgga
     7441 aagtagtggt ccgggctgga ggttattgat ggaacatcat ctggatcagg gcagagagag
     7501 cctggaaagt gatgtggtga ttttcgccac aggttaccgt tctgcattgc cacaaatact
     7561 tccctcactg atgcccttga tcaccatgca cgataagaac acctttaaag tgcgtgatga
     7621 cttcactctg gaatggagtg gcccgaaaga aaataacatc tttgcggtca acgccagcat
     7681 gcaaactcat ggcatcgccg aaccccagct cagcctgatg gcctggcgat ctgcacgtat
     7741 tcttaatcgc gtactggggc gtgatttatt cgatctcagt atgccgcccg cactgattca
     7801 gtggcgcagc ggcagccgga aaaaaccgca gccggaggct gcttctttaa ctcactatac
     7861 agcaaatatt caggaataac gatgatgcgc aaaaagtata tgccccgggc tcttggtccg
     7921 ctgcttcttg tcgtgctgtc accagctgtc gcccagcaaa acgatgataa tgagatcata
     7981 gtgtctgcca gccgcagcaa tcgaactgta gcggagatgg cgcaaaccac ctgggttatc
     8041 gaaaatgccg aactggagca gcagattcag ggcggtaaag agctgaaaga cgcactggct
     8101 cagttaatcc ccggccttga tgtcagcagc cagagtcgaa ccaactacgg tatgaacatg
     8161 cgtggccgcc cgctggttgt cctgattgac ggtgtgcgcc tcaactcttc acgttccgac
     8221 agccgacaac tggactctgt cgatcctttt aatatcgacc atattgaagt gatctccggc
     8281 gcgacggccc tgtacggtgg cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa
     8341 ggtcagccgg aaaccatgat ggagtttgag gctggcacaa aaagtggctt taacagcagt
     8401 aaagatcacg atgagcgcat tgccggtgct gtctccggcg gaaatgacca tatctccgga
     8461 cgtctttccg tggcatatca gaaatttggc ggctggtttg acggtaacgg cgatgccacc
     8521 ctgcttgata acacccagac cggcctgcag cactccaatc ggctggacat catgggaacc
     8581 ggtacgctga acatcgatga atcccggcag cttcaactga taacgcagta ctataaaagt
     8641 cagggagacg acaattacgg gcttaatctc gggaaaggct tttccgccat cagcgggagc
     8701 agcacaccat acgtcagtaa ggggctgaat tctgaccgca ttcccggcac tgagcggcat
     8761 ttgatcagcc tgcagtactc tgacagtgat ttcctgggac aggaactggt cggtcaggtt
     8821 tactaccgcg atgagtcgtt gcggtactac ccgttcccga cggtaaatgc gaataaacag
     8881 gcgacggctt tctcctcgtc acagcaggat accgaccagt acggcatgaa actgactctg
     8941 aacagccaac tgatggacgg ctggcaaatc acctgggggc tggatgctga gcatgagcgc
     9001 tttacctcca accagatgtt cttcgatctg gctcaggcaa gtgcttccgg agggctaaac
     9061 aaccataaga tttacaccac cgggcgctat ccgtcatatg acatcaccaa tctggcggcc
     9121 ttcctgcaat ccagctatga cattaatgat atttttaccg ttagcggtgg cgtacgctat
     9181 cagtatactg agaacagggt agatgatttc atcgactaca cgcagcaaca gaagattgct
     9241 gccgggaagg cgatatctgc cgacgccatt cctggtggtt cggtagatta cgataacttt
     9301 ctgttcaatg ctggtctgct gatgcacatc accgaacgtc agcaggcatg gttcaatttt
     9361 tcccaagggg tggcattgcc ggatccgggg aaatattatg gtcgcggcat ctatggtgca
     9421 gcagtgaacg gccatctttc cctgacaaag agcgtgaacg tcagcgacag taagctggaa
     9481 ggcgtgaaag tcgattctta tgaactgggc tggcgcttta ccggtgacaa cctgcggact
     9541 caaatcgcgg catattactc gctttccaat aagagcgtgg aaaggaataa agatctgacc
     9601 atcagtgtga aggacgacag gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg
     9661 atcccggata ctgactggag taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa
     9721 gtgaacggtc aatggcaaaa atatgacgtg aaggaatcaa gtccatcgaa agcgacagct
     9781 tacattaact gggcgccgga accgtggagt ctgcgtgtac agagcaccac ttctttcgac
     9841 gtaagcgatg cagagggtaa cgatattaat ggttacacta ccgtcgattt tatcagtagt
     9901 tggcagcttc cggtgggaac actcagcttc agcgttgaga acctcttcga ccgtgactat
     9961 accactgtct ggggacagcg tgcacctctg tactacagcc cgggttacgg ccctgcttca
    10021 ctgtacgact acaaaggccg gggccgaacc tttggtctga actactcagt gctgttctga
    10081 cggggatgct tttgcaacaa gggtatgctg taaacaacat ggtccttggt ggccatgttg
    10141 tttacagcca gtataatata cagccttaac aagatattcc gtctccggaa tggactgttc
    10201 accactgaat aacattattt ctgttttatg gatctttgac tgaaatttat cgcatatcct
    10261 catttcattg tccgtctgaa aactaacctt acaagtaagc gtcgtctcag caccgtctgg
    10321 cagatcctga aattcctgag aggatgatct g
//
LOCUS       DAJTMK010000140        10074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTMK010000140 DAJTMK010000000
VERSION     DAJTMK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.142"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            317..1363
                     /locus_tag="OO135_003130"
                     /pseudo
     CDS             317..1363
                     /locus_tag="OO135_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
     gene            complement(1360..2151)
                     /gene="nei"
                     /locus_tag="OO135_003131"
     CDS             complement(1360..2151)
                     /gene="nei"
                     /locus_tag="OO135_003131"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5398888.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2187..2921)
                     /gene="pxpA"
                     /locus_tag="OO135_003132"
     CDS             complement(2187..2921)
                     /gene="pxpA"
                     /locus_tag="OO135_003132"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5398889.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEQALAFARRLRSTFAEKEIVVAA"
     gene            complement(2911..3843)
                     /gene="pxpC"
                     /locus_tag="OO135_003133"
     CDS             complement(2911..3843)
                     /gene="pxpC"
                     /locus_tag="OO135_003133"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5398890.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3837..4493)
                     /gene="pxpB"
                     /locus_tag="OO135_003134"
     CDS             complement(3837..4493)
                     /gene="pxpB"
                     /locus_tag="OO135_003134"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5398891.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGNLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4516..5259)
                     /gene="ybgI"
                     /locus_tag="OO135_003135"
     CDS             complement(4516..5259)
                     /gene="ybgI"
                     /locus_tag="OO135_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5398892.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5530..6921
                     /gene="dtpD"
                     /locus_tag="OO135_003136"
     CDS             5530..6921
                     /gene="dtpD"
                     /locus_tag="OO135_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5398893.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAVGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQIT"
     gene            complement(7053..8471)
                     /gene="phrB"
                     /locus_tag="OO135_003137"
     CDS             complement(7053..8471)
                     /gene="phrB"
                     /locus_tag="OO135_003137"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5398894.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8468..8977)
                     /locus_tag="OO135_003138"
     CDS             complement(8468..8977)
                     /locus_tag="OO135_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5398895.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9261..9635)
                     /locus_tag="OO135_003139"
     CDS             complement(9261..9635)
                     /locus_tag="OO135_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398896.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLILMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9620..>10074)
                     /locus_tag="OO135_003140"
     CDS             complement(9620..>10074)
                     /locus_tag="OO135_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5398897.1"
                     /translation="LGLIDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFG
                     GGGLQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGM
                     GGDYHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2339 a         2743 c         2420 g         2572 t
ORIGIN      
        1 gcctcgcctt ataccttcaa taaatatgct gaatacgccg atttcaccag cgacatgttg
       61 gtctacgaaa aaacctatac agctgaactt tcgtccattg caggtacccc catcgaggct
      121 gggcccttcg atacagtggt gctttttaaa attaactata actgatttgc catgatgccc
      181 tttgcagccg gaaagggcat ctctttcaga aaattgctga ttttcctctt ttccttgatt
      241 actgttattt tttcctggcc cggctttctt tatgctgtat cgatacacca aagagaaaat
      301 aatgagggag cgaaggatgc cagttttgca gtggggaatg ttatgtgtgc tatcacttct
      361 cctttctatt ggtttcctcg cggtacatct cccggcggcg ctattacttg ggccgatgat
      421 cgccgggatc atcttcagta tgcgcggaat tactctgcaa cttccccgct ccgcttttct
      481 cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac ctcactggtt cgattctcac
      541 cacgctggcc gccaactggc cgatcgtgct tgctatttta ctggtgacgc tgctttccag
      601 cgccatcgtg ggttggttat tggtgcgcta tagctcactg cctggaaata ccggtgccta
      661 gggttcttct ccaggcggcg cagcggcaat ggtcgctatg gcgcaagatt acggcgcaga
      721 tattcgcctg gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcgg gggccgctgt
      781 tctggtcacc tgcatgatgc tgggtgataa cgctgaagcg gttaaccagc aaatcgtctg
      841 gttcccgcca gtgagcatta atctcctgct tacgattttg ctcgcagtcg ttgccggtac
      901 ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc atcccgatgc tggcgggcgc
      961 agtgctccag tctggtcagc tcatcaccat cgaactaccg gaatggctgc tggcgatggc
     1021 gtatatggca attggctggc ggattggtct tggtttcggt aaacaaatct tactgcgggc
     1081 actacgccca ctaccgcaaa tcctgctgtc gatttttgct ctgctggcta tttgtgcagg
     1141 tatggcgtgg ggactgaccc ggtttatgca tattgatttt atgactgcct acctcgccac
     1201 cagtcccgac gggcttgata ccgtggcggt catcgccgca gggagcaatg ccgatatggc
     1261 gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg ggccagccat
     1321 tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc tagtgctggc agcctggaca
     1381 ccagtaaaac gggcgcgagg acaacgtggt tttctcaatg atgccgccac agcgttcgca
     1441 cagttcgcca tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat gcttattctc
     1501 atccacctgc ccccgcgtag cgtaagaaaa ccgaggaatc tccagtaacg cgtgtgcgag
     1561 tgcatccagt tgcgccgcat tgagatcttt cgctttatga tttccagtca acccaacctg
     1621 ccagaggatc tccacccgca aataattgcc aagcccagcc agaaacgcct gatcgagcag
     1681 taatcctgca aactgacggt tacgaaagcg cggtgacaat aaccgttcct ttaccacctc
     1741 cggcgtcaga ttcggatcca gcacatcggg gccaacgcgt tgtaaaaacg gatgcgtggt
     1801 cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta taaagcagaa tggttttgtc
     1861 agccgtttgc agttttaccc gcaatactcg cgtggtctgc ggctcttcgc cggtatcaac
     1921 cacgcgccag acgccgtaaa gctgattatg gctgtagagc gttaagtcgt tggaaaaatg
     1981 agttaacaac gccttaccac gcgttcccac atgggtaacg tgttgaccga taagttgtga
     2041 ttgataagtt tttaactgcg ggaaggcaaa ccagacatca gttagtggtt tgcctttgat
     2101 cgccgcctcc aggttatccg ctgcacggcg gatctccggg ccttcaggca tggtgatatc
     2161 cttttttcat tctttattaa tcggggttat gctgcgacaa caatctcctt ttcggcaaat
     2221 gtagagcgta gtcggcgggc gaacgccagt gcttgctcgc cgtcgccatg caggcagact
     2281 gtttgcgccg tgaccgttgc ccattcgccg gtgatacttt tgactctgcc gtgttgcacc
     2341 atctctagcg tttgcgccag cgcctgttct tcgttttcaa tcaacgcgcc tggctgactt
     2401 cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg caaacacttc ctcgcgcgtt
     2461 gtcaggccat attgcttgcc tgcacgaatc agctcgcttc ctgccagccc aacgagaacc
     2521 aatgctggat cgcaagcgta taccgctctg gcgatggcgt ctgccagttg tgcttctttc
     2581 gccgcctggt tgtacaacat gccgtgcggt ttgacatgac gcattacgcc gccttgcgca
     2641 cgggcaatcg ttgccagcgc gccaatttga tacagcgtct gggcgtaaac ggtttctggc
     2701 ggcagctgca tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg gtgagcgcca
     2761 atcgcgacac cattttttat tgcttcacgc acgcaagcct gcatggtttg ggcatcgcct
     2821 gcatgaaatc cacaagcaat attggcagag gaaaccagcg ttaatagctc tgcgtcgctg
     2881 gcgcagcctt cgcccagatc ggcgttcagg tcaattttca ttgtgtagcc gccacgctaa
     2941 ttgttcgaaa taacgttgtt gatcttgccg cgctttcagt gcctcttcca gtgaacactg
     3001 gacaaaatga atcggctgac cgagcggaat ttgcgccaga tggtacatat cagcctcaat
     3061 gatacaggca atacgcgggt aaccaccggt ggtctgtgcg tcgttcatca acacaatcgg
     3121 ctgcccgtta tgcggcacct gcaccacacc cggtaacaaa ccgtgagata acagttcgcg
     3181 atcggtggtg cgttttaaaa tttgcccctg taagcgatag cccatgcggt tactttgcgg
     3241 gctaagctgc cagggcgaac gccagaatgc atcctgcgag gcgcgatcga actcatgata
     3301 ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa cgccctgcgc
     3361 ttccataaaa tcacgcttcg atttgccaat cgggagtcgg tcaccatcct tcagtaaacg
     3421 gccttccagc ccgccaatcc ccacgttgag atcggtgctg catgacccca ttaccggcgg
     3481 aacatcaata ccacccgcga ccgccagata actgcgcatg ccgtgctgcg ggcgtttaag
     3541 cgttaaacgc tggcctgctt tcatcggcaa gcgccagccg gtccagacgg cgttatcatc
     3601 cagccgcgct tcgcaaccgg cacccgtcag agcaaaccac ccatcagttt cgaactcaac
     3661 cgttaactga ccgagcgtga tctccagcgc gggggcattg gcgtcattac ccaccagcag
     3721 gttagcaatg cgtaacgcag gcatatccag tgcgccgcag tggctgatac ccgactggcg
     3781 aaaaccgtga cgaccgccat cctgcacagt ggtatacatg cccgcacgaa taatcttcag
     3841 catactccct ccttctgcgg tacaaagcgc acgctgtctc ccggacgtaa taagatgggt
     3901 tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac caatcaactg ccagccaccc
     3961 ggcgttgcca gcggataaac accagtctgc ggcccgccga tcccgacaga acctgccgga
     4021 acgagcaagc gcggttcagc gcgccgtggc gtgtgtagtt gttccggcaa attcccgaga
     4081 tacgggaagc ccggttgaaa acctaaaaac cagaccacgt attccacgga ggagtgcaat
     4141 tcaacaacct gtttttcgct caacccgcaa tgcgccgcga ccaccgccaa atccggtccg
     4201 cctgcaccac cgtaaaccac cggaatttca ataaagcgag actcaggttc cagggcctcg
     4261 ctctcctccc accagcgttg caaacgctct atggcatcca gcgccagcga ctcaggatta
     4321 cgcaaaatca ccgtgatatt gttcatgccg ggaatggctt caaccacatt cggcatatcc
     4381 accagacgct gcgccagtcg ccagatccgt ttctggctag ccagcgtcac cggcggttcc
     4441 agttccagca ctaccgccgt ttcacctatc agataacaac gcgctcgttg cactttcgat
     4501 ccctctgatt attcgttatg caggattagg aatatcaata aaggtcacat caagatcggt
     4561 attttcattc agccactcgc tcaatgcgcg aataccacca cgttcagtgg catggtgacc
     4621 tgcagcataa aaatgcaatc cctgctcgcg ggctgaatga atggtctgtt cagaaacttc
     4681 gccagtaata aaagcatcca cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc
     4741 gcccgtgcac caggcgacgc gctggaccac ctcaggtccg gtatcgccac accataacgg
     4801 cttacgtccc agacgcgctt caatccagga ggctagttcc agtcccggca caggcatggt
     4861 cagctctccc cacggcacca acggctcaat ttcgcccatg accgtgatcc ccagtaacgc
     4921 cgccagctgt gcgttattgc ccagctcagg atgtgcgtca agcggcaaat gccagccata
     4981 caggttgata tcattcgcca gcaacgtttt taaacggtta cgcttcattc cgcgaatgac
     5041 cggagactct cctttccaga agtagccatg atgcacaatg accgcatccg cgcccagacg
     5101 cactgcctca tcgagcaaag cctggctggc ggtgacaccg gtaacaattt tttgcaccgt
     5161 ctctttgcct tccacctgca aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag
     5221 tttttcgtta atcagttgtt ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg
     5281 atagctacac cgttaacata gcgttacctc gcctgttcgt cgatagccaa tcttttgaca
     5341 tattaagaaa caaacccgac gccaaaagac gcaaattgtt tatatcatca cgcttcagct
     5401 aatgtatatt tatgcaatca gacagcatat tgcataaata atcagatctg tatcaaagtt
     5461 ttaactttaa ggaaacacaa gcatgccgcg caactattac gtgacgttgc cgtcaattgt
     5521 ggaattatta tgaataaaca cgcatcacag ccgcgcgcta tttactatgt cgttgcgctg
     5581 caaatctggg aatattttag cttttacggc atgcgtgccc tgctgattct ctatctcacc
     5641 aatcaactaa aatacaacga tactcacgcc tacgagttat ttagcgccta ctgttcgctg
     5701 gtgtatgtca cgccaatcct cggtggcttt ttggcggata aagttctcgg caatcgcatg
     5761 gcggtgatgc tgggggcgtt attgatggcg atcggtcatg tggtgttggg tgccagtgag
     5821 atccatccgt cattcctcta tctgtccctg gcgataatcg tttgtggcta tggtctgttt
     5881 aaatccaatg tcagttgcct gctcggcgag ctgtatgagc caaccgatcc gcgtcgtgat
     5941 ggcggtttct cgctgatgta tgcagtgggt aacgtggggt ctattatcgc acctattgcc
     6001 tgtggctacg cccaggaaga gtacagctgg gcgatgggct ttggcctggc ggcggtgggt
     6061 atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc atttcacaca tacccgcggc
     6121 gttaataaaa aagtactgcg tgcgacaaac tttctcctgc ctaactgggg atggctgctg
     6181 gttctgctgg tggcaacgcc tgcgctgatt accgtattgt tctggaaaga gtggtcggta
     6241 tacgccttaa ttgtcgcgac tattattggc ctgggtgtac tggcaaaaat ttatcgcaaa
     6301 gcagaaaacc agaaacagcg taaggagctg gggctgattg tgacgctcac cttcttcagt
     6361 atgttgttct gggccttcgc acaacagggc ggcagctcga ttagccttta tatcgaccgc
     6421 ttcgttaacc gcgatatgtt tggttatacc gttccgactg ctatgttcca gtcgattaat
     6481 gccttcgcag ttatgctgtg cggtgtgttc ctggcgtggg tggttaaaga aagcgtcgca
     6541 ggtaatcgta ccgtgcgcat ctggggaaaa tttgctcttg gtctcggcct gatgagcgcc
     6601 gggttctgca ttctgacctt aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg
     6661 ccactgatgg tattaggcct ggcggtgatg ggctttgcgg aactgtttat cgacccggtt
     6721 gccatgtcgc aaattacgcg cattgaaatc cccggtgtga ccggcgtatt aaccggcatt
     6781 tatatgctgc tttccggcgc gattgcgaac tatctcgcgg gcgtgattgc cgatcagaca
     6841 tcgcaggctt cgtttgatgc ttccggggcg atcaactact ccatcaatgc atatattgaa
     6901 gtatttgatc aaattaccta gggcgcactg gcgtgtgtag gactggtact gatgatttgg
     6961 ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc tggagtcata atagaccagc
     7021 accatacctg ataaaacgct cgctgcgttt tatcaggctc ctttcctcgc ttcttcgtac
     7081 gctgccagcg ttcgcagcct tgcctctttg tgatcaacta tcggttgcgg ataatccagc
     7141 atcacacctg ctttctgcgc ccacttccac ggctcatgca ccgctttccc tggtacatcg
     7201 cgcagctccg gtaaccatcg acgaataaac tcgccctcac ggtcaaattt ctcgccctgg
     7261 gttgtcgggt tgaaaatacg aaaatacggc gctgcatcgg ttccggttga agcggcccac
     7321 tgccagccac cgttattggc tgccaaatca ccatcaatca gctgcgacat gaaatatcgc
     7381 tcgccttcgc gccagtcgat caacaaatct ttcaccagaa aactggctgt aatcatccgt
     7441 agccggttat gcatccagcc agtgctgtta agctgacgca tggcagcatc gacaatcggg
     7501 tatcccgttt tgccttcctg ccaggcctgt aaatgtgcgg gattgctctg ccactgcaca
     7561 cgatccgtcc aggcaataaa cggacaatgt ttacacaacg aggggtaata cgtcatcaga
     7621 tgacggtaga actcgcgcca gatcagctca ctaagccaga cactaccggc cccaccgtcc
     7681 agcgcctgcg gctgttccgc caacaagcga tgcaaacact ggcgaggcga taacccgccc
     7741 gttgccaggc tggcggacaa acgactggtg ccttccactg ccggaaaatc tcgttgttgc
     7801 tcatattctc cggcaccgtt ctggcaaaac tggcgtaatt gcgcaatcgc cgctttttct
     7861 tccaccggga aatgcgcagt atcgaaagac tgacgaggat aattcagcgt aatggatggc
     7921 gagggctcta tcgatccgct actacgaact tttggcgcag cgacgcactc cggcatccct
     7981 tcccgcagcc gtttcagcca ggcattctta aaaggcgtaa agactttgta catctcgtga
     8041 ttaccggtca tcaccgcgcc aggcggcagg atcacgctgt catcaaatcc ttcacacacc
     8101 acgttacgca gcgctctttc aacttccaca tcccgcgccc gctcattcac ttcatactga
     8161 tagttataaa acaggtgggt aacgctgttt tccgcgcaca cctgtttaac tatttcgaca
     8221 ctggcgacaa agtcatccac ttcacggaac aataaaggaa tacctttttc cgcaagcgct
     8281 atttgtagcc cattcagttg agcattgata agttcagcct gacgcggcga catgttatgc
     8341 gtcgcccact ggcgtggtgt agcgatatac aacgccagca cgcgtgcaga cgaattgcgg
     8401 caggcggcag ccagtgcgag attatcgtgc aaacgtaaat cctggcgaaa ccagaccaga
     8461 tgggtagtca taaaactcct gaatggcgca agttaatcac acgcggccaa agttcgaaat
     8521 aacgctgccc tgaaagccag gcgtcaggat aaagcgccat atagtgtttg atacgcatca
     8581 gcggcgcaag aagtggttgc tcgccacggc gataactgtc gataagcgca gccagctgct
     8641 ggcgttctgt ggaatcaata tgcgggcgaa aataaccctg aacgtgcatc aaaacattgg
     8701 tgtgatcgcg cgggttggcg gggtgagaaa gcaaaacaac tacgcgttgg cggtactggt
     8761 tgtaatagtc atccagattt tgccactggt gaatatcagc cacgaacggg ccaagtttgc
     8821 ggtactccgg ctgagaatgc gccagaaaga cgagtttata gcggctgtga taatcgagaa
     8881 gcgccccgcg cgttaagcca tgttctttca gtcgatgtaa ctcatgaagg gcaaagatac
     8941 ggattagggt gctgtcatca aatcgttgta gattcattac tgctcctgaa cggtataaag
     9001 gagtaagtgt agacgatggt aaatgaattt tatggggcag agtttttgcg gggaagtatt
     9061 ttgttggtaa aaatattatc aacttcatct gagcgagaat tattctacta tttcccgagc
     9121 tgcattcatt ttttgtattt tttattttta gaaaataaca gttctcaaag cttaattacc
     9181 tcactattaa ttgtatttca aactaacaat cgcactccat tttctctttt taaaaaaata
     9241 aacactgtta aaataaatat ttatttcgtt atcttaaatg ctatttctac atcactcgtc
     9301 cttataatca aattattctt attcatagct atttttgcag gtttaaaata gcggctttca
     9361 ccaaagtcag ccatgagaat tagactacaa gttctcccag cctttgcaca tgagaaaaac
     9421 cacccatcaa ctctatttat tcctgaacct ttaacaccat aataaatcag aatttctggg
     9481 tattgttcat ccttcacgtt ttcatatttt tggtatttaa ttgaaaacca agagtctata
     9541 ctgccaacat cttcaaaaaa tattggagct gatgtttccg catgaacata tgcagaaaat
     9601 gctaacaata tggcaatact tagtttggta aaaatatcgt tccacacttt tcaggataat
     9661 ctattggggg gaaggggtct tttcctctaa atctaattgc tcccactcct tgtttgtcag
     9721 accattcgcc atttgcatcc tgacgatacc agtgataatc ccctcccatg cctataaaat
     9781 tgtgaggacg aataaacaat tgagctttat ggtaaccaga tggacaactc ccattatcta
     9841 aatccttggc accatcattt tttgacgctt caaaaatact accgcaagtg agtttagaaa
     9901 actcttttcc agataattct ccaggttgta atcctccgcc gccaaaaatc ctactcccag
     9961 ggtttccata cctatttaga gcatatgaat aacaattatt tacggcaaga taatacggat
    10021 ctgaatttag gtctcgattg aatactggag caggtttatc aattaagccc agcg
//
LOCUS       DAJTMK010000141        10006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTMK010000141 DAJTMK010000000
VERSION     DAJTMK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.143"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(274..1638)
                     /gene="tdcG"
                     /locus_tag="OO135_003141"
     CDS             complement(274..1638)
                     /gene="tdcG"
                     /locus_tag="OO135_003141"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5398898.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPSPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLVYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1710..2099)
                     /locus_tag="OO135_003142"
     CDS             complement(1710..2099)
                     /locus_tag="OO135_003142"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5398899.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2113..4407)
                     /gene="tdcE"
                     /locus_tag="OO135_003143"
     CDS             complement(2113..4407)
                     /gene="tdcE"
                     /locus_tag="OO135_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5398900.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNVQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4441..5649)
                     /gene="tdcD"
                     /locus_tag="OO135_003144"
     CDS             complement(4441..5649)
                     /gene="tdcD"
                     /locus_tag="OO135_003144"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5398901.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEILMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDRVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5675..7006)
                     /gene="tdcC"
                     /locus_tag="OO135_003145"
     CDS             complement(5675..7006)
                     /gene="tdcC"
                     /locus_tag="OO135_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5398902.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7028..8017)
                     /gene="tdcB"
                     /locus_tag="OO135_003146"
     CDS             complement(7028..8017)
                     /gene="tdcB"
                     /locus_tag="OO135_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5398903.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8116..9054)
                     /gene="tdcA"
                     /locus_tag="OO135_003147"
     CDS             complement(8116..9054)
                     /gene="tdcA"
                     /locus_tag="OO135_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5398904.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9243..9560
                     /gene="tdcR"
                     /locus_tag="OO135_003148"
     CDS             9243..9560
                     /gene="tdcR"
                     /locus_tag="OO135_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5398905.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQEKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRT"
BASE COUNT         2692 a         2514 c         2278 g         2522 t
ORIGIN      
        1 atttctcgct caatattttg tagggcttat tatttttttc cgagccgcat caaggcgata
       61 tgcggtctac gtttttgcag gcgagttatt agaataaaga aatgatacgc gcgaggtcag
      121 agaaaatctt cacaatcaag actttgaaat aacggagatg gataagaatt ttctactaaa
      181 ttaatcgcaa tagaatattc attctctatt tgcggcagat cacaaaaaaa aggtgcacat
      241 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
      301 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
      361 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc
      421 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
      481 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
      541 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgccg ccattgaaca
      601 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
      661 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
      721 cggacgacgg aacttatcgt aataaaccag cactgccgga ataatgccgc acgcaccgtt
      781 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
      841 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag
      901 ctgacgacgc agcgctacgg cacgacgcgg cacattgagc ggactaggca gcacgccttc
      961 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc
     1021 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
     1081 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
     1141 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
     1201 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
     1261 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
     1321 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
     1381 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
     1441 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccat acagatcgac
     1501 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
     1561 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
     1621 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
     1681 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
     1741 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
     1801 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga
     1861 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
     1921 atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca
     1981 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
     2041 ccctgaacat aagggccgat tgcgcctggg gcacgttgcg tttcgataat ctttttcata
     2101 catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
     2161 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
     2221 tttttccggg tgctcgatgg catccagcag catttcccga ttcattatgt tgacgttgag
     2281 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
     2341 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
     2401 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
     2461 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
     2521 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
     2581 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
     2641 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
     2701 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
     2761 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
     2821 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
     2881 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
     2941 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
     3001 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
     3061 gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
     3121 caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat
     3181 cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
     3241 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
     3301 ccccatagtg tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc
     3361 gtccagcccc atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga
     3421 atcaaattcc ggtgtacgca ggaagcgtac catacggatc ttcatgatga agtgatcgat
     3481 cagttcctgt gcctgctgct cattgagtac gccagcttta aagtcgcgct caatgtagat
     3541 atcgaggaac gatgccgtgc ggcccagcga catcgcgccg ccattttgcg atttcactgc
     3601 cgccagataa gcgaagtaga gccactgcac cgcttcttgc acattctgcg ccgggcgaga
     3661 gatatcaaag ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc
     3721 tgccagctcc tcacgcagac ggatggtggc ttccagatcc tcgccttttt ccagacgaga
     3781 ctggagatcg gcaaattgca gttcgcgttc acgtaccaga taactgatgc catacagcgc
     3841 tacgcggcga tagtcaccga taatgcgccc acggccatag ccatctggta aaccggtcag
     3901 cacgccagat ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt
     3961 atgggtttta cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata
     4021 ggcgtggaat gaacttttaa tcatattaat gccaccgaac gggtgtagcg cacgtttcaa
     4081 cggcgcatcc gtttgcaggc caacaatttt ttccagcggc tggttaatat atcccgcatc
     4141 atgagcggta attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt
     4201 ttcgatacgg ataccttcca ttactttttc ccacaattcc gtggtggcag gcgtcgcttc
     4261 ggcgaggaaa gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac
     4321 attaatttcg tttttccagt ccgtaccttt aaagccaagc catgcgtcgg cgtacagctt
     4381 atcgctggta tcaatatcta ccttcatgaa aaataatctc tctacaatac ttcaactaaa
     4441 ttatgcaaat tctgcgggcg cgttaacttt gcctaaatga atggcatcca aagcaatcat
     4501 tttttcttcg ttagtcggaa taacagcgca aatgacacgc gcattttcac tggaaacaat
     4561 tcgctcacca caggagttag agcgattatt catttctgta tcaatctcta cgcctaatac
     4621 agccaaatgt tccatgacca gacgacgaat taagcttgaa ttctctccta ttccgccggt
     4681 gaatataatt ccatccaggc gacgtaatga agctgcgtgt ccggcaatat gacgggcaat
     4741 tcggtgaaca aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt
     4801 ttccagaaca cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt
     4861 cactacgcgt tccaggtcac ccaagctctg gttggtttgg ctggcgaccc aggacatcgc
     4921 accaaaatcg acatcgccac tgcgggtacc catcatcaag ccttccagcg gcgtcattcc
     4981 cattgaggtg tcaacactct gaccgttgcg aactgcgcag attgacgcgc cattgccaag
     5041 atgcgccaca accaggccgg agtcatcttc cgctagattc agcaaagaat gtgcgcgctg
     5101 ggaaacatag cggtgcgacg tgccgtggaa accataacgg cgtacaccta actcttcata
     5161 atatttccac ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt
     5221 atcaaatacc gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact
     5281 taaattggcg taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac
     5341 ctcatcggta ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc
     5401 aattaaggcc actctgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa
     5461 tgcaccttcg tagctgtggt gagccagcgg tgctggctct cccccattta cggataagaa
     5521 tgcattttcc gagttaatac cgtcggcaat acctgacatt aatatttcac agtcgctggc
     5581 atcgagtacg gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa
     5641 ttcattcatc tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca
     5701 ggatggtcag cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg
     5761 ccagagacgg cgctttacgg atggcataca tcggcaacag gcacagcagg gatgcgataa
     5821 tcggtgcgcc catggcttca ataaggtcga ggatgttcgg gttggcgtag gcaacaaccc
     5881 aggtggagcc catgatgaag atcatgctga tagtgttcag tttacccagc gacactttag
     5941 ttttgtcgcc tttataaccg aacttcagaa tcagaccatt caagccttcc agcgtcccca
     6001 gatagtgacc gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt
     6061 ccagtgtaat cgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg ttagccagat
     6121 aagaaagcac tggaatattc tgcgctttgg cttccgccat gttggccgga gacagcgtaa
     6181 acaggcagct aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa
     6241 tgatttggga acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac
     6301 gtttagaaac cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa accatgatgg
     6361 aaatccccag ccagacagtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga
     6421 ggtcaacctg gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca
     6481 ccaggctggc gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac
     6541 caaaccagat gacgaaagcc atcagcagca acaggaacag cgccacaaag ccgcgattca
     6601 gcggtgcaaa tccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc
     6661 cataaatcca cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc
     6721 cagttttacc aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag
     6781 aaagacacag acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca
     6841 acatcagaag aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc
     6901 cggcgccgat tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg
     6961 acgattgttt tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac
     7021 gaattaatta agcgtcaacg aaaccggtga tttgagagac gcgagaaaga tcgatattgc
     7081 cgccggaaat aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta
     7141 ataatgcagc acatgccaga gcgcctgcgc cttcggtgac gactttattg cgctgaatta
     7201 aggcaatcat actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt
     7261 cacgaacgat ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg
     7321 tgccggtagt tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa
     7381 cgttttctga ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg
     7441 caataccagc aattaaacca ccaccaccaa ttggcacaat cacgttatcg acatcataga
     7501 gatcttccat aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt
     7561 aaggtgggat aaaaatacga ccttccattt cgacaatttc gctcacttta gcgatagtgt
     7621 cgttgaagtt atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt
     7681 tggattttgg cgcacctttt ggcatcacca ctttaccgtc gatacccagc atcgcgcagg
     7741 agagcgaaac cccttgcgca tggttgcccg cagaacaggc caccacgcct ttgcgttttt
     7801 ccgcatcggt cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac
     7861 gctgcatatt ttcaaacttc agaaatattt cacctttgca acgttcacta aaatagttgg
     7921 agcgaggcat gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatat
     7981 catcaatagc aaccggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt
     8041 aaatatgtag gtaaccgtaa ccgacacctg caaaacagac aggtggatta tttatgttta
     8101 aataaaaaca aataactaac caacttctat taatagcctt cgtctacacc cattataaga
     8161 tgaatactct ttggctaatt ccaccaaaac cgatgctgct tttttaatac gataattttt
     8221 cgaccatacc gcggcatatt gtgccacagg taatgtttct tcaaccggaa tagtaataaa
     8281 ttgatttgaa ccaaaaggtg acgtcatatc acaaggaatt acagttaaga aatcagcatt
     8341 gagaacaaga ttataaattg tcacgactga gtcggtttta acgatgtttt caatactgat
     8401 gccatttctt tgtaacgtag taagaagctc gctgtagtac cccatattgg tttgtggcaa
     8461 cacccactgt tcgttcttca acgactccag cgtggtggtg ccggtgcatg ttcgggactt
     8521 actggctacc agcacaaact cggactcgaa cagcggctca acatgtaaat cctgaagctt
     8581 catttctgca cttaacgtac caatcgcaaa atccagtcga ccgtcgcgga ttgccggtaa
     8641 gaacgaagac agttgcgctt catacataga aacctgcgct ttcgggaaca cctctttgaa
     8701 cttgttgatc atccctgaca taaaagtaaa accaatcaat gaaggaaaac caaatgagac
     8761 ttccaccacc gcctcagaag acataccgct tatctcatta accatatttt tcatttcacg
     8821 ggtaatggat tcggaacggg agagtaacaa ttgaccggca ggtgttaatg ttacaccggt
     8881 atttttccgc accactaatt ccacaccaaa ataatcttca atatcgttaa tgattttact
     8941 gacggccggt tgagttaacc ctaattcttt tgcagccgag ccgatagagc cacttctaat
     9001 gacttcctga aagactacca ggtgctgcgt tttcggaaga agaatagtgc tcataacgac
     9061 ctacgttaat tacctcattg acggcatgaa gtgtatcaaa atgaaatgaa caggatatgt
     9121 gcgaccactc acaaattaac tttcattact ttccagggta tcgttattac aattaaatat
     9181 ctttaaatat caacaagtta aagcataaaa atcagcataa accctgattt ttatcaaaaa
     9241 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
     9301 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggaa aaatttgagt
     9361 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
     9421 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
     9481 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
     9541 tctcttctga caggacgtga ttttgtgaat gcaatcgttt tccataaatt cttctcccct
     9601 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctggtcgccg
     9661 cgataaggtc agacgaagtc aacaagggga attttcactg tgctttacat cagcgtagga
     9721 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt
     9781 gagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
     9841 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgctg
     9901 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttgcggaaa
     9961 ttgtctccac caatgacgtt aaaaaaattt acagtataag taatga
//
LOCUS       DAJTMK010000142        10000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTMK010000142 DAJTMK010000000
VERSION     DAJTMK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10000
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.144"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(264..1607)
                     /gene="gdhA"
                     /locus_tag="OO135_003149"
     CDS             complement(264..1607)
                     /gene="gdhA"
                     /locus_tag="OO135_003149"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5398906.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1843..2115
                     /locus_tag="OO135_003150"
     CDS             1843..2115
                     /locus_tag="OO135_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5398907.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2081..2488)
                     /gene="nudG"
                     /locus_tag="OO135_003151"
     CDS             complement(2081..2488)
                     /gene="nudG"
                     /locus_tag="OO135_003151"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5398908.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2575..3185
                     /locus_tag="OO135_003152"
                     /pseudo
     CDS             2575..3185
                     /locus_tag="OO135_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
     gene            complement(3194..4501)
                     /gene="ynjE"
                     /locus_tag="OO135_003153"
                     /pseudo
     CDS             complement(3194..4501)
                     /gene="ynjE"
                     /locus_tag="OO135_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(4568..5221)
                     /locus_tag="OO135_003154"
     CDS             complement(4568..5221)
                     /locus_tag="OO135_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5398909.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5221..6757)
                     /locus_tag="OO135_003155"
                     /pseudo
     CDS             complement(5221..6757)
                     /locus_tag="OO135_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(6730..7896)
                     /locus_tag="OO135_003156"
                     /pseudo
     CDS             complement(6730..7896)
                     /locus_tag="OO135_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(7906..8454)
                     /locus_tag="OO135_003157"
     CDS             complement(7906..8454)
                     /locus_tag="OO135_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5398910.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8454..9161)
                     /locus_tag="OO135_003158"
     CDS             complement(8454..9161)
                     /locus_tag="OO135_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5398911.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9176..9853)
                     /gene="ydjY"
                     /locus_tag="OO135_003159"
                     /pseudo
     CDS             complement(9176..9853)
                     /gene="ydjY"
                     /locus_tag="OO135_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9858..>10000)
                     /locus_tag="OO135_003160"
     CDS             complement(9858..>10000)
                     /locus_tag="OO135_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5398912.1"
                     /translation="RFILQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKN
                     EG"
BASE COUNT         2380 a         2721 c         2588 g         2311 t
ORIGIN      
        1 atctgctgct atcgcagaaa gttaacggat gctgaacttg aagcgtgtta tcgttacgac
       61 aagcagtttt gggagcacta cagtaagaaa tgccgttggc ccataagaaa tgttccctgg
      121 ggagcatcat ccgttaaata ctcataaacg cctgaaattt tgccgggggc gctgcgcttg
      181 cccggcctac attagagcgt aactgcaatg aattgtgccc atttgtaggc ctgataagcg
      241 tagcgccatc aggcatttac aacttaaatc acaccctgcg ccagcatcgc atcggcaacc
      301 ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg tttgctcacc ttcaccacca
      361 tgctcaacac aggcatggtg gatatccagc atgatgtgat gcaaacgtgc gtcaactttc
      421 tcggctttcc agcccaggcg cgcagcgttt tgcgccattt caaggcctga cgttgctaca
      481 ccgccagcat tagccgcttt acccggtgca aacagtacgc ctgcctgctg gaacagttca
      541 gtcgcttcga tggtggtcgg catatttgcc ccttcggcga cggctttaac gccattggcg
      601 ataagctgat gcgcggcgtc aacatccagt tcgttctggg tggcgcaagg cagggcgata
      661 tcaaccggta cagaccacgg ctgttggcct tcgagataga ccagaccaaa ttctttggcg
      721 taatctgcca ctcgaccatc gcggctggct ttgatttcga taagacgtgc cagtttctct
      781 ttcgtgaatc cgctttcatc aactacagtg ccgctggagt ctgacgcagt gatcacacga
      841 gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga cgttgccgga gccagaaacg
      901 gaaacgcgca tcccttcaaa acccataccg tggcgtttta acattgcttc tgtgaaataa
      961 accagaccgt agccggtagc ttccgggcga ataagactac cgccaaatga aaggccctta
     1021 ccggtgaaga cgcaggcggt attgttggag agctttttca tcatccccgc cataaagccg
     1081 acttcacgac caccaacgcc gatatcacct gccggaacgt cggtatccgc gcccagatga
     1141 cgataaagct cggtcatcag cgcctggcag aaacgcatca cttcgctttc gcttttacct
     1201 ttcgggtcaa aatcgctacc gcctttacca ccgcccatcg gcagggtagt cagggcattt
     1261 ttgaaggttt gttcgaagcc gaggaatttg agaatggaga ggttaactga cggatggaag
     1321 cgcataccgc ctttgtacgg gccgatggct gagctgaact gcacacgcca tgcacggttg
     1381 acctgtacct ggttgcgatc atcaacccat accacgcgaa actggatcac gcgctccggt
     1441 tcaaccagac gctccagtaa tgacatctgg cgatattttg gattttgttc aagaaaaggc
     1501 cagagtgtgg tcattacttc acgaacggct tgcgcgaact cggtttgatt cgggtcgcgc
     1561 ttttggacat ggttgaggaa tgactccaga gaatatgtct gatccataga tataaaaccc
     1621 ttatatatta atacgattgt gcttatgttg tttatgtcat gtgcttttgc agttttcgac
     1681 tataccatca agaatgtgac ggtaagcaag aaaaaaatga cccaggaacg caaattaata
     1741 acgagagtaa tctcataacc aaatgcgata actcagacgc taaagttttg ccacccggct
     1801 accgttatca gttgcagaga caataacgtt aaggagttcc ctgtgagtcg agcattgttc
     1861 gccgtagtat tagcatttcc attaattgca ctggctaacc cgcattatcg tccggacgtg
     1921 gaggtaaacg taccgccaga agtgttcagt tcaggcgggc agtcagcgca gccctgtact
     1981 cagtgctgcg tttatcagga tcaaaattat tctgaagggg cggtaattaa agccgaagga
     2041 atactgctgc aatgccagcg ggatgacaaa acccttagca ctaatccgct gctctggcgg
     2101 cgcgtaaagc cataaacgcc tctaatagtg gaatgtcagt aggggccagc ggatattgca
     2161 gcgcctcttc aggtgagcac cagaccagcg cctgatgttc atgtgcctgt aacgtcccgt
     2221 ggaagtcggg tacgtgccag gcatgaagat ggataatccg gcccgaaact tctcgctgat
     2281 ggctggcaac atactcaccc acagttgctt cgatgcccaa ttcttcgttt aactcacgca
     2341 ccagtgcctg ctgctggctt tcatccagct cgactttacc accggcaaac tcccataatc
     2401 ccgcctgatc gctctgggcg gggcgttgcg cgagtaaaat tttgccatca cgttcaatga
     2461 tggcggcaac aacttcaatc attttcataa attacacacg ggcagataag cgaaaaatgc
     2521 cataatatcc gcactgttgc tcaatcagaa aggctttata tttaaggagt gcctgtgcta
     2581 gaccgccatc ttcatccccg gattaaaccg ttgttgcatc agtgcgtgcg ggttcttgat
     2641 aaaccgggca ttacgccaga tggtttaaca ttagtgggat ttgccatcgg cgtgctggct
     2701 ctgccgtttc tggcgctggg ctggtatctg gcggcgctag tcgtcatttt gttgaacagg
     2761 ctgcttgatg gtctggacgg tgcgctggcg cggcgcagag agcttaccga cgcgggcggc
     2821 tttcttgata tttctctcga ttttctcttt tacgcgctgg tgccgtttgg ctttattctt
     2881 gctgcaccgg agcaaaatgc gctggcaggc ggctggttgt tgtttgcgtt tatcagcacg
     2941 gcagcagttt tctcgccttt gctgcactgg cagcaaaaca tcagattgat aaccccggtt
     3001 atgcacataa gtcgttttac tatctggggg gattaaccga aggcacagaa acgatcttac
     3061 tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg gatcttcggt gcgttgtgct
     3121 ggatgacgac ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc
     3181 agtaattgca atgttatttg ctactgtccg ggccgcgttc accggttgct accggatttt
     3241 ttggatcgct gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca
     3301 tggcgcgtgc gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa
     3361 cttgctgctc tggtttgata ttccacgctt tccacatagc ggtaatatca tcggcgctgc
     3421 gcatggtgcc atccgggtta tgaaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc
     3481 cccaacgtgc tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac
     3541 cgataaattc tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc
     3601 cacgggcctg ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat
     3661 ccggttccgc tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc
     3721 aggtttgcca gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt
     3781 gcgccacacg cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc
     3841 gaatgccgtg ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg
     3901 gttcgctttc cacttcgttg gtatcgatgt agtcagcacc gggaatatgg ctgataaggt
     3961 aaagcttagg agcgccctag gccgcttcaa tgactttcca gtcaccggca ggtttcgccg
     4021 taacctcttt accttgttgc aggtcgtgca gccattgcgg ataaaccagc tgctcaaagt
     4081 gcggcagttt ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg
     4141 taaaacctgc tttttgcagt cgcgttttga cggcgtcgac atctttgtca ttaccgtaca
     4201 gcgccaccgg ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg
     4261 tgctcatttt gtcgagccag ctggcagaga ggtttaaggc ggcaggttca tgaccagaag
     4321 ggccatttaa ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt
     4381 tgccattttg ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg
     4441 aagaagcgca agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca
     4501 tcgaaaatcc cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa
     4561 ctttgcgtta tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac
     4621 tatcagcagg aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagttcgc
     4681 gaacttcgct gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga
     4741 atggctcatc caggagtaac gcttttggct gagcgagaag ggcgcgcagc agggccaccc
     4801 gcgctcgctg accgcctgat aatgtcgcag ggtcctggtg ataagtttca gccagtcccg
     4861 ctcgatcaag cgcgtctttc acggcattgc gcctggcagt ccccttgagc gtcgacggta
     4921 gcgccagcag taaattttgc ccgacgctga actggtcaaa taacagtgca tcctggaaaa
     4981 gaatgccaat ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata
     5041 gctcacctgt acaagaaaac tgtccagcca gtgcaccaat catccatgaa aacagagtgg
     5101 attttccaca gccagacggc cccattaacg tgacgatgtc acctttatca accgtaaagt
     5161 taacgtttgt cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca
     5221 ttagcggagt ccttgtctga cataacctac ccattttgcg actaacgcgg tcagggcaaa
     5281 aataataagc ggtaacaaca gttgccataa agcctgggcg gcgagaatac cgttgctgcc
     5341 gccgctgctt aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca
     5401 cagcgtcggc atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac
     5461 agggcgcaac atgagtgggc atttcacgta aaagaagatt ttggcccgcg accagcccag
     5521 tgtttgcgca atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa
     5581 cagcatccac ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc
     5641 caggttcagc catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag
     5701 aatgggcaac catagccaca actggcgatg ctgtggcccc cattccagcc acagcagtaa
     5761 caggagcaaa gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc
     5821 actattgatc gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt
     5881 taagggtaaa aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcattgcgaa
     5941 tgccatctac gcggggaata gtgcgccgcc agctgcgcca tagcagatag ctcagcaaaa
     6001 cgtaggcggc gagtaacagc atcagcagca gactggcgag tgcgcctttc gtttgttgat
     6061 ccgcgtcgcc ctgagttaac cactgccagc taattactgc cagcgtcggt ggattacctg
     6121 gcccaagaat aatcgccaca tccacgaccg aaagcgacta ggcaacaatc gccagcatcg
     6181 ccattgccag cgaaggcgcg acggagggca acagcagcca gttcaggcat tgccagcggc
     6241 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     6301 ccgctaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     6361 tgccaaatcg gtccattggc ggagtgaaat acgggaaata gtcatatagc agccctccgt
     6421 cagcaaagag cagcagggcg ctggtggcaa aagccacatg gggaatggcg agcagccacg
     6481 gcagacgggc gcacatacgc tgccatttcg gcccaggcca tagcgccaca atcaccagca
     6541 gggcaatcaa caatgccccg accgccgcga tggttgtcga caccagcgtt gccagtaatg
     6601 catgcggtaa ctgtggatcg gcaaataacg cctgccagtg tgtcaacgac aacgcaggcg
     6661 atatcagcgt gagagctgcc gggatcagcg gtgcataaat caccgccacc atcgcccaca
     6721 gcaaaaagat taatgcgtac cgtaacggcg taaccattct tgttccagtg cattcaccca
     6781 tcctgcgtgc ggttcagcca gtaccggcgg cagatcctgc ggcattcttg attgcaatat
     6841 ttcgcgctgc ccgtcaggca gtttttgcgg atcgagaaca gaaggatcgc cccagacaac
     6901 gggatctgct ttacgcagtt gcgcatcggg tgagagcaga aaattggcaa ctaccttcgc
     6961 cgcagcactg gcattcgcgt tggctggaat ggtgacgaaa tgcacgttgc caatcatccc
     7021 ctcgcgaaag ccaaaactgt aactgcttgc cggcaaatcg ccgctggcga ttttttgctg
     7081 cgcatgcgcg gggttaaagg tcagcgacag gcgcaatgtg ccggctttca gcagagcatc
     7141 catccgcgcg ggcgaaggcg ggaaatcctt tccttcgcgc cacaaatacg gatgcagtgc
     7201 atcaagatat tgccacaagg gcgcagtgac acgggcgaaa gtcgcatcgt ccggcgcttc
     7261 ttttaatgcg gcgggatctg gcgtcagcat aatcagcaac tgttcaagaa acgccgtgcc
     7321 ggtaaagtcc ggtgggcgcg gataagtaac cgtgtcggga ttggctttag caaactccag
     7381 taaggcttgc ggcgtttgtg gtggctgtgg cgtaacatcg cggcgggcga taaacgtcag
     7441 ttgtgcgccg ccctagggcg attccgcacc ttgtgtcggc actgaaaaat cttcccgcac
     7501 cggcagctgt gtgtcgacat agcgccagtt gggcagagtc tccgcccagc ccgtttgtaa
     7561 taaattggcc tcttttaagg tgcggaagtt ttcgccgttc acccagagca gatccaccga
     7621 gccgcccgtt ttacgaccgg cagcagcttc ggtctgaatg cgcttcaccg cgtctgcggc
     7681 atccgcgaga cgaacaatct tcaggtttat agcgtaatgg gttttcatct cgccgctaac
     7741 ccagtcgagg tagcggttaa ttgcggtatc gccgccccag gcgttaaact agacggtttg
     7801 ccctttggcc tcatttttaa tttcctgcca gtcactggca tgtgttgcca aagaaaacag
     7861 cgataacaat cccagcaacc acccacaatg gcgcatcttc tctccttaag aacgtttttc
     7921 cggaatacgg cacagcccct gagcgggaat gtccatggca gaattaaaac gagccgacag
     7981 gttttgcagg ccgatcaatg ccgtcagttc ggtgagcgcc tgagcgtcaa aatgcgcagc
     8041 cagtcgggta cgcagggcat catcgactgt tggcggtgtg acgcttgcgg cttcggcgta
     8101 ctccagcgcc agccgttctt catcgctaaa gagcgggctt tgccgccagt cagccactgc
     8161 cagcagttta tcggtgctgc cggtacgctc ggcgactttc atactggtga tatccacaca
     8221 aaactcacac aggcacattt gcgcaatgcg cgcgctgaca agcgatcgca ctaccggatc
     8281 gagcggtgag cgtttgcgct ccagccaccc aacaaacatt gacaccaaat aaaagataaa
     8341 cgggatccgc ccccaccagc gaatcgggct tagcacctcg ccgtaatggc gacgatgcag
     8401 ccacgcctgc ggacgtaaaa tgaaaggaat tttgctaagc ggcggtaaac ccatcagtca
     8461 ttcctcttct ggcgctcaag ccatattttc ttcgccataa aaatcaccac ggtcagggca
     8521 aacagaataa acagtccggt gacaaaccag aaagtaccgc ctgttaacat gctgcccgcc
     8581 caggaataaa caatagtcgc cggtaattga ccaagtccgg tggcgataaa aaacgagcga
     8641 aaacgtattg aagtcagacc ggcagcatag ctgattggat cgaaagggac aaaaggcaat
     8701 aaccgacaga ccaggatggt gtgtttgccg tagcgagtga aaaagccgtc catactgtca
     8761 agcacggttt tgccggttaa tttttccacc acttcgcggc ccatcactct ggcgataaaa
     8821 aagcacagcg ccgcgccggc catcgaactg gtccacgaca gcaagccgcc ccagaacgcg
     8881 ccaaacagcg acgcattggc aaaggtgatc aaaaacgcag gtagcggtgc agcaatggcc
     8941 tgtaaaatca tcaacaagaa cgagacaact gccgccagtg cgccgtaaga ctgaataaag
     9001 cgttctatac cctgttggtc cacggcggca aacgccgcaa cgctgcgatt gataaactca
     9061 tggacgccgg gaagcagcgc ccatgcgagc agcatagcga acaacaggat gatgagggtt
     9121 atacggtagt accagatttt acgcgattgc atcttcatca tcctttatcc tggctttatt
     9181 cggtgatttt aaaggtaacc gttgccagcg tgttatccgc cgggagaact gaggcattgc
     9241 ctttgaattt cacttcgccg cgtttttcca ccgcaccata agtgtatgtt gcattgctga
     9301 cgatgccgac cgggcagcta tccagacaca ccagtcaacc cgttttcttc tcttctgctg
     9361 ccgttaaatt accgccaaag cgcatgtcca gtttcttgcc attactgtca acaatcactt
     9421 catcgaagga atacgctttt gccgcccctt gccagttgac cgaaatatcc agtttgctgc
     9481 ctgtgacatg agtcgtttct ttattatcca tcgtcatgtt ttcgcccggc gtaccacctg
     9541 cctctttcag ggcttcataa aatgctttcg gggtcgcata agccataaac agcgatttat
     9601 gtccgttgga gccatcttta aacacaatac cgtgacgagt gtcgtcggtg aggaaacgac
     9661 cattaatttg caccagcatg gttaccgtat ggttattggc atcgacgcgc agcggttggc
     9721 tgttcgaaag tccgtcgtat tccacttttg ccgccgcatt ctctttttga tcgcagccgc
     9781 taagcccggc aacagccagt aagcagagtg cagccagtcc ttttttccat gacactgaat
     9841 aatgttgcga catcgttcta tccttcattt tttgggtgag taagcgggct gcggcgcgaa
     9901 gcagacagat ccacatgttt gtgtcgggcg tagagttttg cgagctggac gagaataaac
     9961 agcgccaggc ccgccagaca gagttgtaaa ataaagcgca
//
LOCUS       DAJTMK010000143         9914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTMK010000143 DAJTMK010000000
VERSION     DAJTMK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9914
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.146"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..565)
                     /locus_tag="OO135_003161"
     CDS             complement(<1..565)
                     /locus_tag="OO135_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5398913.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     YAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGH"
     gene            complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO135_003162"
     CDS             complement(784..1761)
                     /gene="epmA"
                     /locus_tag="OO135_003162"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5398914.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGSETLAEVIAFSVDRA"
     gene            2086..3894
                     /gene="frdA"
                     /locus_tag="OO135_003163"
     CDS             2086..3894
                     /gene="frdA"
                     /locus_tag="OO135_003163"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5398915.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVPIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3887..4621
                     /gene="frdB"
                     /locus_tag="OO135_003164"
     CDS             3887..4621
                     /gene="frdB"
                     /locus_tag="OO135_003164"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5398916.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIEHD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4632..5027
                     /gene="frdC"
                     /locus_tag="OO135_003165"
     CDS             4632..5027
                     /gene="frdC"
                     /locus_tag="OO135_003165"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5398917.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5038..5397
                     /gene="frdD"
                     /locus_tag="OO135_003166"
     CDS             5038..5397
                     /gene="frdD"
                     /locus_tag="OO135_003166"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5398918.1"
                     /translation="MINPNPKRSGEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5460..6590
                     /locus_tag="OO135_003167"
     CDS             5460..6590
                     /locus_tag="OO135_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5398919.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
     gene            6679..7212
                     /gene="blc"
                     /locus_tag="OO135_003168"
     CDS             6679..7212
                     /gene="blc"
                     /locus_tag="OO135_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5398920.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO135_003169"
     CDS             complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO135_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5398921.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO135_003170"
     CDS             complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO135_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5398922.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO135_003171"
     CDS             complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO135_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5398923.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO135_003172"
     CDS             complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO135_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5398924.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8750..9799
                     /gene="epmB"
                     /locus_tag="OO135_003173"
     CDS             8750..9799
                     /gene="epmB"
                     /locus_tag="OO135_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5398925.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
BASE COUNT         2543 a         2555 c         2518 g         2298 t
ORIGIN      
        1 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
       61 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccctttgaag
      121 caacgacggt gaccagaatc atccatgcta ctgccagcag accaaccacc tgcgtaggct
      181 ccagtccggc aatacgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
      241 gtacccaaac tttcgcgtag gtactcacca tccagatgat ataagaggaa aaccacataa
      301 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccac
      361 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
      421 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      481 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      541 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      601 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      661 atactcggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      721 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      781 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg agcccagcgc
      841 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      901 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      961 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
     1021 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     1081 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     1141 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
     1201 gtcttcttcg gtatcagcaa cgttactcaa atccagtttc gctgcgactt cccgcagttg
     1261 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     1321 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1381 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     1441 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1501 ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1561 ctgcgaatgc ccggggccta cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1621 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcag caaagaaacg
     1681 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1741 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1801 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1861 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1921 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1981 gttgtaccta taaaggagca gttgaatagc gttcgcagac cgtaactttc aggcacttac
     2041 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     2101 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     2161 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     2221 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     2281 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     2341 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     2401 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2461 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2521 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2581 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gccgatccgt
     2641 gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta caacaccaac
     2701 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2761 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2821 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2881 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2941 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     3001 tccacgccgc gtggcgatgt ggtttatctc gacttgcgtc acctcggcga gaaaaaactg
     3061 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     3121 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
     3181 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     3241 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     3301 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagaggca
     3361 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
     3421 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctag caatggaaga aggttgcggt
     3481 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3541 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3601 accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3661 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3721 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3781 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3841 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
     3901 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3961 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     4021 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     4081 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     4141 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacacg atctggtggt
     4201 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     4261 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     4321 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4381 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4441 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
     4501 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4561 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4621 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4681 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4741 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
     4801 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4861 tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4921 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4981 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     5041 attaatccaa atccaaagcg ttctggcgaa ccggtattct ggggcctctt cggggccggt
     5101 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     5161 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     5221 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     5281 cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa atgggttttc
     5341 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac aatctaacgt
     5401 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5461 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca tttgctgccc
     5521 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5581 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5641 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5701 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5761 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5821 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcagatg ccggatgaag
     5881 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5941 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     6001 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     6061 accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg ggatatcgcg
     6121 aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     6181 cgaccattga agatatggcc cgctgggtgc aaagcaattt aacccccctt gatatcaatg
     6241 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     6301 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6361 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6421 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6481 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6541 ccaatccagc gagagtcgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     6601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     6661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     6721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtagt gaataatttc
     6781 gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     6841 cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atggcggcgg cctgaatgtc
     6901 atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac
     6961 tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     7021 ggttataacg tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac
     7081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     7141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     7201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     7261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
     7321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
     7381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
     7441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     7501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
     7561 aaaagacgcg tttgcaggtc gtcctgcaac gctgatggtt gaacggatat tttgccaggt
     7621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
     7681 cagctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
     7741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     7801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     7861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     7921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     7981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
     8041 gtgagcagcg tactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     8101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     8161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
     8221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     8281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     8341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
     8401 ttatcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     8461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     8521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     8581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     8641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     8701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     8761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
     8821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
     8881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
     8941 gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt
     9001 cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt
     9061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
     9121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
     9181 gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
     9241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
     9301 catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg
     9361 catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatcc tgctggtgaa
     9421 tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg
     9481 ccgggtaggc gttactttgc tgaaccagag cgttctgtta cgtgatgtga acgataacgc
     9541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
     9601 gcatgtgctc gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg
     9661 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
     9721 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagttact
     9781 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
     9841 tcgtgcattg aaattgaatg gatttgaaat gaactgtagg ccggataagg cgtttacgcc
     9901 gcatccggca agag
//
LOCUS       DAJTMK010000144         9428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTMK010000144 DAJTMK010000000
VERSION     DAJTMK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9428
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.147"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            106..417
                     /gene="istB"
                     /locus_tag="OO135_003174"
                     /pseudo
     CDS             106..417
                     /gene="istB"
                     /locus_tag="OO135_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            691..873
                     /locus_tag="OO135_003175"
     CDS             691..873
                     /locus_tag="OO135_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5398926.1"
                     /translation="MNHPVIGLVTKADLASMEHISLVKCWLREAGAHNVLVTSAVNNN
                     GVTELFSLLHTEDVCR"
     gene            complement(974..1303)
                     /locus_tag="OO135_003176"
     CDS             complement(974..1303)
                     /locus_tag="OO135_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5398927.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1475..2533)
                     /locus_tag="OO135_003177"
     CDS             complement(1475..2533)
                     /locus_tag="OO135_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5398928.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVIFGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(2731..3204)
                     /gene="sbmC"
                     /locus_tag="OO135_003178"
     CDS             complement(2731..3204)
                     /gene="sbmC"
                     /locus_tag="OO135_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR5398929.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(3322..4488)
                     /gene="dacD"
                     /locus_tag="OO135_003179"
                     /pseudo
     CDS             complement(3322..4488)
                     /gene="dacD"
                     /locus_tag="OO135_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
     gene            4698..6122
                     /gene="sbcB"
                     /locus_tag="OO135_003180"
     CDS             4698..6122
                     /gene="sbcB"
                     /locus_tag="OO135_003180"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5398930.1"
                     /translation="MNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMNPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(6165..6392)
                     /locus_tag="OO135_003181"
     CDS             complement(6165..6392)
                     /locus_tag="OO135_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5398931.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(6406..7464)
                     /gene="yeeE"
                     /locus_tag="OO135_003182"
     CDS             complement(6406..7464)
                     /gene="yeeE"
                     /locus_tag="OO135_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5398932.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWPGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITPPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(7643..9001)
                     /gene="plaP"
                     /locus_tag="OO135_003183"
     CDS             complement(7643..9001)
                     /gene="plaP"
                     /locus_tag="OO135_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5398933.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(8991..9053)
                     /gene="yoeI"
                     /locus_tag="OO135_003184"
     CDS             complement(8991..9053)
                     /gene="yoeI"
                     /locus_tag="OO135_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5398934.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9268..>9428)
                     /locus_tag="OO135_003185"
     CDS             complement(9268..>9428)
                     /locus_tag="OO135_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5398935.1"
                     /translation="GSITAQTLFEPVWIGWNEQTAGLASGWWRDEILANSAIAGVYAK
                     SDDGKSAI"
BASE COUNT         2593 a         2410 c         2180 g         2245 t
ORIGIN      
        1 actcctccgg cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca
       61 gcctgagttc acagataaaa cactctccag gaaacccggg gcggttcact tcagcgtgga
      121 gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt cagtcaggaa
      181 gaagcaaaac tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc aatgatcctg
      241 acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc agcactgacc
      301 tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa aggagaaagc
      361 tatcgactca gacagaaacg aaaggccggg gttatagctg aagctaatcc tgagtaaaac
      421 ggtggatcaa tattgggccg ttggtggaga tataagtgga tcacttttca tccgtcgttg
      481 acacttggca aacaggggca accatgtatg tcagatatta caatctcact tccgaaagaa
      541 gtcaacgggt acgttatctg ttcaaccaga atccgacaca ttctggctat acgaaagagc
      601 atacttatgc aaatctatat gcttttccgg caacaagcag taatctcagc gctgaggcag
      661 gaattagagg taaaaacgcc ctaccgggcc atgaatcacc cggtcattgg attagtaacc
      721 aaagcagatt tagccagcat ggaacacata tctttggtga aatgttggtt acgggaagca
      781 ggagcgcaca acgtgttagt aaccagtgcg gttaataaca atggagttac agaacttttt
      841 tccttgctgc atacagaaga tgtctgtcgc taatttcgat aggttctcag atgagaatga
      901 tgacggtgat acacagataa gaatcaatga gcaatgaggg ttgccgggca accctcattg
      961 aataaaacag gaattacttc gcttcgccgt ttttcatttc gcccatagct ttcagctttt
     1021 tggagatatc gcggcgttct ttggagagct cggcattttt gatgatgtaa tcgtcaacac
     1081 gatcttcata gtcacctttc atgctggcga tgatgccctg gattgcttca acgctcatcc
     1141 ccggcttgat gtaatcgctc aggttgtcca gcagcaggac gcgcttctgg ttgtcacgga
     1201 tctttttctc aacgtcctga atttcacgtt gcagtttgtt cttacgacgg aacagacgaa
     1261 caaattccag tacgtcctgg aatgaaggct tggtagtttc catttgtata cccctgataa
     1321 tgtgagagtc ggattcgttt tagcaaccgc tattcgttag ggccaacatt actcatagct
     1381 gccgcagatg acaatgcttt tatccctttt cactatcata ccctttatcc ttgctgaatc
     1441 gaagcagcag caagatgatt ctgaagttca ggaattattt gcgcaaggcc cggcaaatca
     1501 gattcgatag caactcaagc tgatgtgccg tttccatgtt cagccacaca taaccataga
     1561 ttggtgtttc cactaccttc aggtcatggt ggttattgag caactgacgc agctcttcca
     1621 cagcatcgtt caatttttcc gtattggcaa aaaccggctg cggattacct tcgtacagcg
     1681 catgaacaag gctcagcagt atttgctgca tcatgtgctg ggtatcacga agtttttgcg
     1741 cgttcaataa cacgaaatgg ctggggcgcg tggcccagta cgcattgatt tgcaactcca
     1801 gcatacaaac cagattgcgg ttaatggtct ggataccttc atatatcgat tttggaatac
     1861 gggtttcttt gctggcgggc gcaatcagcc cacgcatttt cacggcatcg gtcaggagtt
     1921 tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat gcagattgat
     1981 aaacccgatt atactcggtc agacttttcg ccagttgaat gcgccagtgg atgaacgccc
     2041 gttgtggcca gataccggta aacaacattg ccagtaaaga gccgaagatc acatcgccgc
     2101 ttcgccataa cgccgtatca atttcacctg tcggggaacc cacaacaatt gccagcgtca
     2161 ccccaatcaa taatccttga tacggtttct tgcccagcgc cagccaaccg caaagaaaca
     2221 tagccgccgc gcaccagact aacatcagcg gtaacgaaat taactccagt tgcagagcga
     2281 taagacctaa aatcgaaccc aacaccgtac cgccaatacg ttcgaaggcg cgagggacaa
     2341 cgttacccca gaacgagatt ggccccataa tcaccaccat ggtgaccagc ggccaggtgc
     2401 tttccgggat agtaaacagg cggataatga gaaaagtgag caggaatgcc agcgcgaccc
     2461 gagtaccatg cacaatgcgg taatggcggt atacccggat ttcaaacggg cttaatgact
     2521 tatcggcacg cacgcagaaa ctccgtaatg aagattaaaa gcgctcataa ctcatctgtc
     2581 ggccagaagg cccgcctctg aattatccga cggacaaacc gggaacagtg taatttcccg
     2641 gcaggagcga aaaaattgaa aggcgcatct tactcttttc gctttcaaaa aaagagtggt
     2701 catcgcgtta acacaccgcc ctgagatgaa ttagtgatgt tttggctgca ccgcaacata
     2761 catttcgata tcccagtacc catcttccgc gccattgttc aaataaacct cgaagcaggg
     2821 ctttggtaac atttcataag cactgtcctg caagaggcta ttaaagaact gataccaggg
     2881 tttagcaaaa tcatcaccga ctacacgagc caccgcaacc gcatactgac cacctgaaat
     2941 ttctgtcaga atgacgccct cactgttttc ggggagcgta aagttattcg gcaccgtcac
     3001 gacggtgtcg cagcgtaatt tttcggcggg tgtttcatct ggattgtcgt aaaagacagc
     3061 aacccactcc ttcggcacaa tatttttgct atctacccac atcatcaact gctcaaagcc
     3121 tttctttacc gtctgttccc acgggccaac gagatggaaa cctgcaacgg tacgtttatc
     3181 ttcctgctta atctcgtagt tcatgacgct ccatcaacac tgtttttata tacagtataa
     3241 ttgcgatatt aaaaccacaa ggaatgagtg ttgattatgc gagcagactc gcactcctgc
     3301 cagtctgctg caaaagaaag gtcaggcctt atggtggaaa taatcactca ggcgagaaaa
     3361 catgctgcct tccccgacag attccagggt aaccagcggc cagtgcgcca cctgtttatc
     3421 acggtcgtaa agttcaattt cccctacccg ctgatgggcg ctaattggcg cggtgagctc
     3481 tttaccatca agggtatatt tggctttgat atgtggaatt tcggctttcg gtagcaccat
     3541 ccagaactct tgttccgttc ccaggtcgat attttcttta tcgccatacc agatgcgttc
     3601 cgtaccgacc tttttcccac ggtgcaaaat ttgcaccgta gtaaagtttt gttgccccca
     3661 acgcagtaat tttcttgcct cttcctcacg accttttgca ctgtcagccc ccattaccac
     3721 tgcaatgaga cgacgctgcc catcgacagc cgaagcaatg agattaaacc cggcaccaga
     3781 agtatgaccc gttttcaggc cgtcaacatt catggtttta tcccacaata acccgttacg
     3841 gttttgctgg gtgataccgt tccaggtgag acttttctca ctgtacatat gataaaactc
     3901 gggctcgccg tggatgatag cgcgagaaag cacagctaaa tcataagccg agctatgctg
     3961 acctggtgca tccagaccat gcactgtttc aaaatgcgta tccttgagat gcagcttctc
     4021 ggcatagttg ttcatcattt caacaaactg ccgttgccca ccggcaatat agtcagccag
     4081 tgcaacacaa gcgtcatttc cggaatccac aattaaacca cggcttaaat cacgtaccga
     4141 tacgcgatcg ccctctttca aaaacatcag tgaagaaccg acaaacactg gattatcttt
     4201 cgcccacgca tcgcgcccca cggtgacaat atcgtctggc gtaatgcgat gactatcgat
     4261 agtgcgatcc acgacatagc ccgtcatcag ctttgtcagg ctggcgggat tgcgctgttg
     4321 atgctcatta cccgcggtga ggatctgacc ggtggtgtaa tccatcagta cctaggagcc
     4381 ggcatgaatc tctggaggct gaggtgaaaa aggaatgttt tccgccgcaa aaccagacga
     4441 taagttaaaa atgaacaaag aagcagcaat aataagacgg cgtttcaaca gcaaaccctc
     4501 aggagtttca aatagctgtt ctttttacgg aaatacttat gaactggctg gaataaagtg
     4561 caagaaaatg tgactaccct ctcattttta tctgacatga tctgttgtca ctcgctgtca
     4621 aattgtcgcg ctaaagctga ttagcacggt gatatttgat actctggcag acagcagaaa
     4681 taacggattt aacctaaatg aatgacggta agcaacaatc tacctttttg tttcacgatt
     4741 acgaaacctt tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca
     4801 ccgatgacga attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgcggatg
     4861 actatttacc ccagcctgga gccgtattaa ttaccggtat taccccgcag gaagcacggg
     4921 cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga
     4981 agacctgtat tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt
     5041 tttatcgtaa tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg
     5101 atttactgga tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aattggcctg
     5161 aaaatgatga cggtctacca agctttcgcc ttgagcattt aaccaaagcg aatggtattg
     5221 aacatagcaa cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcaaaac
     5281 tggtaaaaac gcgtcagcca cgcctgtttg attatctctt tacccatcgt aataaacaca
     5341 aactaatggc gttgattgat gttcctcaga tgaaccccct ggttcacgtt tccggaatgt
     5401 ttggggcatg gcgcggcaat acaagctggg tggcaccgct ggcgtggcat cctgaaaatc
     5461 gcaatgccgt aattatggtg gatttggcag gagacatttc gccattactg gagctggata
     5521 gcgacacatt gcgcgagcgt ttatataccg caaaagccga tcttggcgat aacgccgccg
     5581 ttccggttaa gctggtacat atcaataaat gtccggtact ggcccaggcg aatacgctac
     5641 gcccggaaga tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctaaaaa
     5701 ttctgcgtga aaatccgcaa gtgcgtgaaa aagtggtagc gatattcgtg gaagccgaac
     5761 cgtttacacc ttcagataat gtggatgcac agctttataa cggctttttc agtgacgccg
     5821 atcgtgcagc aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata
     5881 tcacttttgt tgataaacga attgaaaagc tgttgttcaa ttatcgggcg cgcaacttcc
     5941 cggggacgct ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca
     6001 cgccagagtt tttacaaggc tatgcagaag agattcagat gctggcgcag cagtatgccg
     6061 acgataaaga aaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagattgtct
     6121 aatgtgaagc ctccgccgct ggtacggcgg aggacaattt tgagtcaggc tttttgaacg
     6181 gtgatgctcc acgctgcatc gccaatctgc tgataatcgg tgatggcatg tccttcttct
     6241 gccgcccact gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca
     6301 tcgccgctgg ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc
     6361 tgcgtcacca catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc
     6421 agttgccagt cgtgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac
     6481 tccgagaatc ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcatgac
     6541 cagtccatta ccgatagagc aaccacctgc aatgctggca ccgaagccca tcagtacacc
     6601 acctaacccg ctacgtaatg ttgtttgtgc atcagcagcg cgaacgcgga actcacggct
     6661 cgctttggct gcaataaatg accccacgaa gatccctaac accaggaaaa cgccccagtt
     6721 aatgtatttc acgtcacccg cgaccagaaa ttgcagaata ttggccgttg ggggagtgat
     6781 tcctaaccca aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa
     6841 accgatgagt acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat
     6901 cccggttcgg cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat
     6961 caccacccag agcgttatca ccagcaaaac ggcaaccaac ggccacacag acaaattaaa
     7021 agtatcagca atagagttat gttcagtagt gtagtgctgc aaggtttgat ttaaaccact
     7081 ggcgtgtgga gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact
     7141 gccgatcaat ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac
     7201 aattcccagc ccgaaaagat acccacctat aacagtacca ggccacggga acgccccggc
     7261 ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat
     7321 tgcaatcagc aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc
     7381 acctgtcagg caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag
     7441 cccgcttaat atcattgaaa acataaatta attaaccaga tgaatgttaa tggggaaatt
     7501 attcatgact ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta
     7561 ttaatgagga tttcgataaa aaagaataaa aaaaccggag ccaaatgttc attcgactcc
     7621 ggtttattat tagaaaagat gattatgcta cgtcttcgta ctgcggaacc ggattacgga
     7681 aactcttggt tacgcaagcc aggtaaatca gaccgatagc agcccagatc agacccagaa
     7741 ccattgagct ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg
     7801 gcaggaacag atactggaag tgatctttca gcgtcttgtt acgcttctca cggatccaga
     7861 actgcgaaat aaccgacagg ttaacgaagg tgaacgccac cagcgcacca aagttaatca
     7921 gcgccgtagc cattaccagg tcgaagttga ttgccagcaa ggcaatcgcg ccaaccagga
     7981 tgatgttcat tgccggagta cgccatttcg ggtgtacata accgaagaag cttttaggga
     8041 acacaccgtc acgccccatt acgtacatca gacgcgctac gcctgcatgc gccgccatac
     8101 cggacgccag tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg
     8161 caacgtacag catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg
     8221 ggaagtacag ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg
     8281 tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacaggttgc
     8341 tgatgccgtc aaagccggta aaggagaagc acaggattgt cgccccggta atcatcggga
     8401 taacatgcgc atcgccagac cagaacggac gagtgctcgc cagcgtaccc gcgccttcac
     8461 cttcaaatac gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca
     8521 cgacgattac agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca
     8581 taaaggccac cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa
     8641 aatagatttt cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag
     8701 accaacccac cataaagccg acagtcgggc taatggattt ctgggcgtaa gtgtatgcag
     8761 agccagcaga aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg
     8821 caattaatgc gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac
     8881 caaatgtatc aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa
     8941 ctaacgtaag cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca
     9001 tggtcattct cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg
     9061 gattcctcag cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg
     9121 cctcttggta tgaatgattg gtttgaagca aaaaataacc gacgctaatt aaacgtcggt
     9181 ttttagtcat tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa
     9241 tgagaggctt gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc
     9301 ataaacaccg gcgatcgcac tatttgctaa aatttcgtcc cgccaccaac cactggcaag
     9361 ccccgcggtc tgttcattcc agccaatcca gacgggttca aagagggttt gggcggtaat
     9421 gctgccaa
//
LOCUS       DAJTMK010000145         9379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTMK010000145 DAJTMK010000000
VERSION     DAJTMK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9379
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.148"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..467
                     /gene="wcaE"
                     /locus_tag="OO135_003186"
     CDS             <1..467
                     /gene="wcaE"
                     /locus_tag="OO135_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5398936.1"
                     /translation="FHQDAANFVRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGCY
                     IYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFS
                     MGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            483..1031
                     /gene="wcaF"
                     /locus_tag="OO135_003187"
     CDS             483..1031
                     /gene="wcaF"
                     /locus_tag="OO135_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5398937.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            1058..2178
                     /gene="gmd"
                     /locus_tag="OO135_003188"
                     /pseudo
     CDS             1058..2178
                     /gene="gmd"
                     /locus_tag="OO135_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            2181..3146
                     /gene="fcl"
                     /locus_tag="OO135_003189"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             2181..3146
                     /gene="fcl"
                     /locus_tag="OO135_003189"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5398938.1"
                     /translation="MSRQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            3149..3628
                     /gene="gmm"
                     /locus_tag="OO135_003190"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             3149..3628
                     /gene="gmm"
                     /locus_tag="OO135_003190"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5398939.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3625..4848
                     /gene="wcaI"
                     /locus_tag="OO135_003191"
     CDS             3625..4848
                     /gene="wcaI"
                     /locus_tag="OO135_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5398940.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAYTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            4851..6278
                     /gene="cpsB"
                     /locus_tag="OO135_003192"
     CDS             4851..6278
                     /gene="cpsB"
                     /locus_tag="OO135_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5398941.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAAKRH
                     SPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRR
                     GEVSAGEQDTVAFEVAQFVEKPNLKTAQAYVASGEYYWNSGMFLFRAGRYLEELKKYR
                     PDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAGWSD
                     VGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAV
                     LIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPG
                     EGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLD
                     LIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6471..7841
                     /gene="cpsG"
                     /locus_tag="OO135_003193"
                     /gene_synonym="manB"
     CDS             6471..7841
                     /gene="cpsG"
                     /locus_tag="OO135_003193"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5398942.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLVLAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEGRFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKDKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7977..9371
                     /gene="wcaJ"
                     /locus_tag="OO135_003194"
     CDS             7977..9371
                     /gene="wcaJ"
                     /locus_tag="OO135_003194"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5398943.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGRAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
BASE COUNT         2240 a         2385 c         2673 g         2081 t
ORIGIN      
        1 tttttcatca ggatgccgca aattttgtcc gtaagttaaa aatgcaaaaa gataacatga
       61 tgatcaccgg cgatgcgctg ctggattttg gcgacgggca taaaattaaa cgtagcgcca
      121 aaccgggctg ttatatttat cacagcctgc ccgccagtca tcaggcgata tttttccctg
      181 tatccggctt gaaaaaatgg cgttatgacc tggaatataa agtttcttcc gactacgcgc
      241 tggcagccaa aatgtataaa gccggttatg catttaaaaa actcaatggc ctggtgtctg
      301 aattttccat gggtggggta tcaaccacca ataatatgga attgtgtgct gacgcgaaaa
      361 aagtccaacg acaaatatta catgtacctg gcttttgggc tgaattatcc tggcatttac
      421 gccaacgtac tacctcaaag acgaaagcct tatataacaa agtctgaata taaggagaac
      481 caatgcaaga tttaagcggt ttctcggtgc cgaaagggtt ccggggcggc aacgctatta
      541 aagtgcaatt atggtgggca gtacaggcaa caatatttgc ctggtcgcca caagtattgt
      601 atcgctggcg ggctttttta ttacgtttat tcggggcaaa aataggaaaa aacgtagtta
      661 ttcgtccgtc agtaaaaatt acctatccgt ggaaattaac cttaggtgat tacgcgtggg
      721 tcggagatga ggtcaattta tataccctcg gtgaaataac cattggcgca cattcggtga
      781 tatcgcaaaa aagttattta tgcaccggta gccacgacca tgcaagtcaa catttcacca
      841 ttaacgccac gcctattgtg attggcgaga aatgctggct ggcaaccgat gtttttgttg
      901 cccctggcgt cacgatcggc gacggcaccg tcgtgggggc gcgaagcagt gtttttaaat
      961 cgcttccggc aaatgtggtt tgccggggga atcccgcagt ggtgatacgc gaacgcgttg
     1021 aaactgaata aattcaaaaa atacagagga ataagacatg tcaaaagtcg ctctcatcac
     1081 cggtgtaacc ggacaagacg gttcttacct ggcagagttt ctgctggaaa aaggttacga
     1141 ggtgcatggt attaagcgtc gtgcatcgtc attcaacacc gagcgcgtgg atcacattta
     1201 tcaggatccg cacacctgca acccgaaatt ccatctgcat tatggcgacc tgagtgatac
     1261 ctccaacctg acgcgcattt tgcgtgaagt gcagccggat gaagtgtaca acctgggcgc
     1321 aatgagccac gttgcgcttt cttttgagtc accggaatat accgctgacg tcaacgcgat
     1381 gggtacgctg cgcctgctgg aggcgatccg cttcctcggt ctggaaaaga aaacccgttt
     1441 ctatcaggct tccacctctg aactgtacgg tctggtgcag gaaattcagc agaaagaaac
     1501 cacgccgttc tacccgcgat ctccgtatgc ggtcgccaaa ctgtatgcct actggatcac
     1561 cgttaactac cgcgaatcct acggcatgta cgcctgtaac ggtattctct tcaaccatga
     1621 atccccgcgc cgcggcgaaa ccttcgttac ccgcaaaatc acccgcgcaa tcgccaacat
     1681 cgcccagggg ctggagtcgt gcctgtacct cggcaatatg gattccctgc gtgactgggg
     1741 ccacgccaaa gactacgtaa aaatgcagtg gatgatgctg caacaggaac agccagaaga
     1801 tttcgttatc gcgaccggcg ttcagtactc cgtacgtcag ttcgtggaaa tggcggcagc
     1861 acagctgggc atcaaactgc gctttgaagg cacgggcgtt gaagagaagg gcattgtggt
     1921 ttccgtcacc gggcatgacg cgccggcgtt aaaccgggcg atgtgattat cgccgttgac
     1981 ccgcgttact tccgtccggc ggaagttgaa acgctgctcg gcgacccgac caaagcgcac
     2041 gaaaaactgg gctggaaacc ggaaatcacc ctcagagaga tggtgtctga aatggtggct
     2101 aatgacctcg aagcggcgaa aaaacactct ctgctgaaat ctcacggcta cgacgtggcg
     2161 atcgcgctgg agtcataagc atgagtagac aacgcatttt tatcgctggc catcgcggaa
     2221 tggtcggttc cgccatcacg cggcagctcg aacagcgcgg tgatgtggaa ctggtattac
     2281 gcacccgcga cgagctgaac ctgctggaca gccgcgcggt gcatgatttc tttgccagcg
     2341 aacgcattga ccaggtctat ctggcggcgg cgaaagtggg cggcattgtt gccaacaaca
     2401 cctatccggc ggatttcatc taccagaaca tgatgattga gagcaacatc attcacgccg
     2461 cgcatcagaa cgacgtgaac aaactgctgt ttcttggatc gtcctgtatc tacccgaaac
     2521 tggcaaaaca gccgatggca gaaagcgagt tattgcaggg cacgctggag ccgaccaacg
     2581 agccttacgc cattgccaaa atcgccggga tcaaactgtg cgaatcatac aaccgccagt
     2641 acggacgcga ttaccgctca gtcatgccga ccaacctata tggcccgcat gacaacttcc
     2701 acccgagtaa ttcgcatgtg atccctgcat tgctgcgtcg cttccacgag gcgacggcac
     2761 agaatgcacc ggacgtggtg gtatggggca gcggtacacc gatgcgtgaa ttcctgcacg
     2821 tcgatgatat ggcggcggcg agcattcatg tcatggagct ggcgcacgaa gtctggctgg
     2881 agaacaccca gccgatgctg tcgcacatta acgtcggcac gggcgttgac tgcactatcc
     2941 gcgagctggc gcaaaccatc gccaaagtgg tgggttacaa aggtcgggtg gtttttgatg
     3001 ccagcaaacc ggatggtacg ccgcgcaaac tgcttgatgt gacgcgcctg catcagcttg
     3061 gctggtatca cgaaatctca ctggaagcgg ggcttgccag cacttaccag tggttccttg
     3121 agaatcaaga ccgctttcgg gggtaatgat gtttttacgt caggaagact ttgccacggt
     3181 agtgcgctcc accccgcttg tctctctcga ctttattgtc gagaacagtc gcggcgagtt
     3241 tctgcttggc aaaagaacca accgcccggc gcagggttac tggtttgtgc cgggagggcg
     3301 cgtgcagaaa gacgaaacgc tggaagccgc atttgagcgg ctgacgatgg cggaactggg
     3361 gctgcgtctg ccgataacag caggccagtt ttacggtgtc tggcagcact tttatgacga
     3421 taacttctcc ggcacggatt tcaccactca ctatgtggtg ctcggttttc gcttcagagt
     3481 agcggaagaa gagctgttac tgccggatga gcagcatgac gattaccgct ggctgacgcc
     3541 ggacgcgctg ctcgccagta acgatgttca cgctaacagc cgcgcctatt ttctagctga
     3601 gaagcgtgcc ggagtacccg gattatgaaa atactggtct acggcattaa ctactcgccg
     3661 gagttaaccg gcatcggcaa atacaccggc gagatggtgg aatggctggc ggcacaaggt
     3721 catgaggtgc gggtcattac cgcaccgcct tactacccgc aatggcaggt gggtgagaac
     3781 tattccgcct ggcgctacaa acgagaagag ggggccgcca cggtgtggcg ctgcccgctg
     3841 tacgtgccaa aacagccgag caccctgaaa cgcttgttgc atctcggcag ttttgccgtt
     3901 agcagtttct ttccactgat ggcgcaacgt cgctggaagc cggatcgcat tatcggcgta
     3961 gtgccaacgc tgttttgcac gccgggaatg cgcctgctgg cgaagctctc tggtgcgcgt
     4021 accgtgctgc atattcagga ttacgaagtg gacgccatgc tggggctggg ccttgccgga
     4081 aaaggcaaag gcggcaaagt ggcacagctg gcaacggcgt tcgaacgtag cggactgcat
     4141 aacgtcgata acgtctccac gatttcgcgt tcgatgatga ataaagccat cgaaaaaggc
     4201 gtggcggcgg aaaacgtcat cttcttcccc aactggtcgg aaattgcccg ttttcagcat
     4261 gttgcagatg ccgatgttga tgcccttcgt aaccagcttg acctgccgga taacaaaaaa
     4321 atcattcttt actccggcaa tattggtgaa aagcaggggc tggaaaacgt tattgaagct
     4381 gccgatcgtc tgcgcgatga accgctgatt tttgccattg tcgggcaggg cggcggcaaa
     4441 gcgcggctgg aaaaaatggc gcagcagcgt ggactgcgca acatgcaatt tttcccgctg
     4501 caatcgtatg acgctttacc cgcactgctg aagatgggcg attgccatct ggtggtgcaa
     4561 aaacgcggcg cggcagatgc cgtattgccg tcgaaactga ccaatattct ggcagtaggc
     4621 ggtaacgcgg tgattactgc tgaagcctac acagaactgg ggcagctttg cgaaaccttt
     4681 ccgggcattg cggtttgcgt tgaaccggaa tcggtcgagg cgctggtggc ggggattcgt
     4741 caggcgctcc tgctgcccaa acacaacacg gtggcacgtg aatatgccga acgcacgctc
     4801 gataaagaga acgtgttacg tcaatttata aatgatattc ggggataatt atggcgcagt
     4861 cgaaactcta tccagttgtg atggcaggtg gctccggtag ccgcttatgg ccgctttccc
     4921 gcgtacttta tcccaagcag tttttatgcc tgaaaggcga tctcaccatg ctgcaaacca
     4981 ccatctgccg cctgaacggc gtggagtgcg aaagcccggt ggtgatttgc aatgagcagc
     5041 accgctttat tgtcgcggaa cagctgcgtc aactgaacaa acttaccgag aacattattc
     5101 tcgaaccggc agggcgaaac acggcacctg ccattgcgct ggcggcaaaa cgtcatagcc
     5161 cggagagcga cccgttaatg ctggtattgg cggcggatca tgtgattgcc gatgaagacg
     5221 cgttccgtgc cgccgtgcgt aatgccatgc cgtatgccga agcgggcaag ctggtgacct
     5281 tcggcattgt gccggatcta ccagaaaccg gttatggcta tattcgtcgc ggtgaagtgt
     5341 ctgcgggtga gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa aaaccgaatc
     5401 tgaaaaccgc ccaggcctat gtggcaagcg gcgaatatta ctggaacagc ggcatgttcc
     5461 tgttccgcgc cggacgctat ctcgaagaac tgaaaaaata tcgtccggat attctcgatg
     5521 cctgtgaaaa agcgatgagc gccgtcgatc cggatctcga ttttattcgt gtggatgaag
     5581 aagcgtttct cgcctgcccg gaagagtcgg tggattacgc ggtcatggaa cgtacggcag
     5641 atgccgttgt ggtgccgatg gatgcgggct ggagcgatgt cggctcctgg tcctcattat
     5701 gggaaattag cgcccacacc gccgagggca acgtttgcca cggcgatgtg attaatcaca
     5761 aaactgaaaa cagctatgtg tacgccgaat ctggcctcgt aaccaccgtc ggggtgaaag
     5821 atctggtagt ggtgcagacc aaagatgcgg tgctgattgc cgaccgtaac gcggtgcagg
     5881 atgtgaaaaa agtggtcgag cagatcaaag ccgatggtcg ccatgagcat cgggtgcatc
     5941 gcgaagtgta tcgcccgtgg ggcaaatatg actctatcga cgcgggcgac cgctaccagg
     6001 tgaaacgcat caccgtgaaa ccgggcgagg gcttgtcggt acagatgcac catcaccgcg
     6061 cggaacactg ggtggttgtc gcgggaacgg caaaagtcac cattgatggt gatatcaaac
     6121 tgcttggtga aaacgagtcc atttatattc cgctgggggc gacgcattgc ctggaaaacc
     6181 cggggaaaat tccgctcgat ttaattgaag tgcgctccgg ctcttatctc gaagaggatg
     6241 atgtggtgcg cttcgcggat cgctacggac gggtgtaaac gtcgcatcag gagctgtttg
     6301 tcggatgcgg cgtgaacgcc ttatccgacc tacggttcgg gtttgtagtc ctgataagac
     6361 gcggcagcgt cgcatcaggc aatgaatgcg aaaccgcggt gtaaataacg acaaaaacaa
     6421 aattggccgt ttcggtcagg gccaactatt gcctgaaaaa gggtagcgac atgaaaaaat
     6481 taacctgctt taaagcctat gatattcgcg ggaaattagg cgaagaactg aatgaagata
     6541 tcgcctggcg cattggtcgc gcctatggcg aatttctcaa accgaaaacc attgtgttag
     6601 gcggtgatgt ccgtctcacc agcgaaacct taaaactggt gctggcaaaa ggtttacagg
     6661 atgcgggcgt tgacgtgctg gatatcggta tgtccggcac cgaagagatc tatttcgcca
     6721 cgttccatct cggcgtggat ggcggcattg aagttaccgc cagccataat ccgatggatt
     6781 ataacggcat gaagctggtt cgcgaggggg ctcgcccgat cagcggagat accggactgc
     6841 gcgacgtcca gcgtctggct gaagccaacg actttcctcc cgtcgatgaa accaaacgcg
     6901 gtcgctatca gcaaatcaac ctgcgtgacg cttacgttga tcacctgttc ggttatatca
     6961 atgtcaaaaa cctcacgccg ctcaagctgg tgatcaactc cgggaacggc gcagcgggtc
     7021 cggtggtgga cgccattgaa ggccgcttta aagccctcgg cgcacccgtg gaattaatca
     7081 aagtgcacaa cacgccggac ggcaatttcc ccaacggtat tcctaaccca ctactgccgg
     7141 aatgccgcga cgacacccgc aatgcggtca tcaaacacgg cgcggatatg ggcattgcct
     7201 ttgatggcga ttttgaccgc tgcttcctgt ttgacgaaaa agggcagttt atcgaaggct
     7261 actacattgt cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc gcgaagatca
     7321 tccacgatcc acgtctctcc tggaataccg ttgatgtggt gactgccgct ggcggcacgc
     7381 cggtgatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag gaagacgcca
     7441 tctatggtgg cgaaatgagc gcccaccatt acttccgtga tttcgcttac tgcgacagcg
     7501 gcatgatccc gtggctgctg gtcgccgaac tggtgtgcct gaaagataaa acgctgggcg
     7561 aactggtacg cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac agcaaactgg
     7621 cgcaacccgt tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag gcgctggcgg
     7681 tggatcgcac cgatggcatc agcatgacct ttgccgactg gcgctttaac ctgcgcacct
     7741 ccaataccga accggtggtg cgcctgaatg tggaatcgcg cggtgatgtg ccgctgatgg
     7801 aagcgcgaac gcgaactctg ctgacgttgc tgaacgagta atgtcattac tttccccctc
     7861 accctgacgc tctactgcct gcggagagaa gtgagcacgg aactttcttc tccccctcgc
     7921 ccctttgggg agagggccgg ggtgaggggc gaggcgacaa tagaacagga acaacgatga
     7981 caaatctaaa aaagcgcgag cgagcgaaaa ccaatgcatc gttaatctct atggtgcaac
     8041 gcttttcaga tatcaccatc atgtttgccg gactatggct ggtttgcgaa gtgagcggac
     8101 tgtcattcct ctacatgcac ctgttggtgg cgttgattac gctggtggtg ttccagatgc
     8161 tgggcggcat caccgatttt tatcgctcat ggcgcggtgt tcgggcagcg acagaatttg
     8221 ccctgttgct acaaaactgg accttaagcg tgattttcag cgccggactg gtggcgttca
     8281 ataatgattt cgacacgcaa ctgaaaatct ggcttgcgtg gtatgggctg accagtatcg
     8341 gactggtggt ttgccgttca tgtattcgta ttggtgcggg ctggctgcgt aatcatggct
     8401 ataacaagcg catggtcgcg gtggcggggg atttagccgc cggacaaatg ttgatggaga
     8461 gcttccgtaa tcagccgtgg ttagggtttg aagtggtggg cgtataccac gatccaaaac
     8521 cgggcggcgt ttctaacgac tgggcgggca atctgcaaca gctggtcgag gatgcgaaag
     8581 cgggcaagat tcataacgtc tatatcgcga tgcaaatgtg cgacggcgcg cgagtgaaaa
     8641 aactggtcca tcaattggcg gataccacct gttcggtgct gctgatcccc gatgtcttta
     8701 ccttcaatat tctccattca cgtctcgaag agatgaacgg cgttccggtg gtgccgctgt
     8761 atgacacgcc gctttccggg gttaaccgcc tgataaaacg tgcggaagac attgtgctgg
     8821 cgacgcttat tctgctgctg atctccccgg tgctgtgctg tattgcgctg gcggtaaaac
     8881 tcagttcacc agggccggtt attttccgcc agactcgcta cggcatggat ggcaagccga
     8941 tcaaagtgtg gaagttccgt tccatgaaag tgatggagaa cgacaaagtg gtgacccagg
     9001 cgacgcagaa cgatccgcgc gtcaccaaag tggggaactt tctgcgccgc acctcgctgg
     9061 atgaattgcc gcagtttatc aatgtgctga ccggggggat gtcgattgtc ggtccacgtc
     9121 cacacgcggt ggcgcataac gaacagtatc gccagctcat tgaaggctac atgctgcgcc
     9181 ataaggtgaa accgggcatt accggcaggg cgcagattaa cggctggcgc ggcgaaacgg
     9241 acacactgga gaaaatggaa aaacgcgtcg agttcgacct tgagtacatc cgcgaatgga
     9301 gcgtctggtt cgatatcaaa atcgttttcc tgacggtatt caaaggcttc gttaacaaag
     9361 cggcatattg atatgagct
//
LOCUS       DAJTMK010000146         9345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTMK010000146 DAJTMK010000000
VERSION     DAJTMK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9345
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.149"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..290
                     /locus_tag="OO135_003195"
     CDS             <1..290
                     /locus_tag="OO135_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoglutarate transporter"
                     /protein_id="HCR5398944.1"
                     /translation="AAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVA
                     NAIFGGSAEYVALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(287..610)
                     /locus_tag="OO135_003196"
     CDS             complement(287..610)
                     /locus_tag="OO135_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5398945.1"
                     /translation="MRILFVCSLLLLSGCSHMDNDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(656..2011)
                     /gene="pssA"
                     /locus_tag="OO135_003197"
     CDS             complement(656..2011)
                     /gene="pssA"
                     /locus_tag="OO135_003197"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5398946.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(2125..4785)
                     /gene="pat"
                     /locus_tag="OO135_003198"
     CDS             complement(2125..4785)
                     /gene="pat"
                     /locus_tag="OO135_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5398947.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSASAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(4817..5515)
                     /gene="tapT"
                     /locus_tag="OO135_003199"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(4817..5515)
                     /gene="tapT"
                     /locus_tag="OO135_003199"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5398948.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(5584..6003)
                     /gene="trxC"
                     /locus_tag="OO135_003200"
     CDS             complement(5584..6003)
                     /gene="trxC"
                     /locus_tag="OO135_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5398949.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            6210..7247
                     /locus_tag="OO135_003201"
     CDS             6210..7247
                     /locus_tag="OO135_003201"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5398950.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(7295..7984)
                     /gene="ung"
                     /locus_tag="OO135_003202"
     CDS             complement(7295..7984)
                     /gene="ung"
                     /locus_tag="OO135_003202"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5398951.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPRQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            8289..8672
                     /gene="grcA"
                     /locus_tag="OO135_003203"
     CDS             8289..8672
                     /gene="grcA"
                     /locus_tag="OO135_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5398952.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(8728..9315)
                     /gene="eamB"
                     /locus_tag="OO135_003204"
     CDS             complement(8728..9315)
                     /gene="eamB"
                     /locus_tag="OO135_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5398953.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2336 a         2398 c         2431 g         2180 t
ORIGIN      
        1 atgccgcttt tggtctggtg atgtgtgccc tgctgatagt gagtttttat acatcaatca
       61 gtggaatact gaaggctgag atgttcccgg cacaggttcg cgcattaggc gttggtctgt
      121 catatgcggt cgctaatgct atatttggtg gttcggcgga gtacgtagcg ttgtcgctga
      181 aatcaatagg aatggaaaca gccttcttct ggtatgtgac cttgatggcc gtggtggcgt
      241 ttctggtttc tttgatgcta catcgcaaag ggaaggggat gcgtctttag tgacgggtca
      301 gttgccagac ggtatagccg gtgcttgcac cggcgacatc ccaggccaaa tccttccagc
      361 tccagccgct cccttcgggg cggctatccc aaagctcttt tgacgccccc aaactgacag
      421 agaacatcaa tccaaacatg gcactgcgat cccggctcat cccctgatgc tgtgaatatt
      481 catttccggc ggcggaaagc atcgccgagg cgataaagtg ttgagcttta tcctgcccgc
      541 tccagctatc gttatccata tggctgcatc cagaaagtaa caatagcgaa cagacaaaaa
      601 gaatacgcaa aacgtggcct ccaaaaaaca aaccccgtac aggacggggt tgtgattaca
      661 ggatgcggct aattaatcgg tcgatgcgga tacggcgcaa acggcggatg agtttacgaa
      721 ccttcaccgg ataatcggca atactttgca gatcgcgata gtgcttaacg atggtggtat
      781 gctcgcggat cagctccagt tctttctctc gctgcggcgc aagctccagt tgtgggtcgt
      841 ggatcaaaat ggcgttttcc agatccagac gccaggcgcg cgggttcagg ttattaccgg
      901 tgatcaacat ccacttatca tcaacccaca tccctttcag gtgataggtg ttgtcgtcat
      961 ctttccataa ccgaaccact agctggtcag tattgacgta atactgcaaa cggctcagga
     1021 aaagacgcag attgatctca tagagataag gcaatgcgcc aattatcttg aaaggttcat
     1081 cttccggaat gtagaagtcg ttcgcggttt tatcaccaac aataatttcg acctttttcc
     1141 cttcgcgcag caactggata atattgcgca caaggattgc tggcaggttg aagtatggcg
     1201 tacagatggt tagtttctgc tcggcacaag gcataagatg gaaaatggtc ttgttcaaca
     1261 gactcgattt ccccagcccc actagcggcg ttacggaaag ctgatcgttg tcggcatcgc
     1321 cctggaaatg ataagcggca tcacgcagct cctggcggaa cagacgaata tcgttcttga
     1381 tttccgggct ttttggccga ttaacatcat ccagacgatt aacgccgcgg ccattcataa
     1441 tattctgtgt aacccattca aacataatgt ctgacatctt acggttacgg ataagatgat
     1501 aacggtcgta gcgatattta tcgtgctgat gcaggtaaac atcgttcagg ctggcaccgc
     1561 tataaagtac gctatcgtcg atgataaagc ctttaaagtg cagaacacca agggcttcac
     1621 gggtattgat tggaacgcca taaaccggaa catctacgcc cgaattttcc tgcgccatgc
     1681 ggcagtacca gtcagcgtta gtgttagatg ccgcagcgcc aatgcgtcca cgttgtgcac
     1741 gatgccagtc gaccagcacc cgcacatcca gttccggacg ctgcctttta gcttcataca
     1801 acgcgttcag aatgcctttg ccaccgtcat cctgttcgag atacagggcg acaatgcaaa
     1861 tgcgctgctt cgcgctggct attttttcca gcagcgtctc ccggaagtcg gcgggagcgt
     1921 aaaagaaatc gacatcatca actgattgag aaatcttggg tagttgggca aggtgttgtt
     1981 gatgtttatt acgcttaaat tttgacaaca tcacagtgca tttcttctct gttcattgaa
     2041 gggtcctctg tgcaatgcag acgacataag cggccaataa taacaccagt cccgattaag
     2101 tggtcaacat ttccagtacc ttactcatga ttcctcgcgt tgggcaagat ttagcgtaag
     2161 cccaacgatc ccctcttcga gctggatatc aacgttaaac ccgagcttgc gggctagcgc
     2221 caccatgcca cgattgtttg gcatcgtaat accattcaga cgttgtagtc cgtgatctcg
     2281 cgtataggta atcaactttt ccattaagcg tcgacctaag cctaaccctt tgagatccga
     2341 gcgaaccagc acagcaaatt cggcatcgat gttatcagga tcggaaatcg cacgcgtgac
     2401 gccgaggatc tcttccgttt gatcaatacg tcgtaccgct acaaacgcca tttcccgatc
     2461 gtagtcgatc tgtgtcatgt tggctaaatc ttcatgggta aattcgttga tctcgctaaa
     2521 gtagcggtaa taaagatctt ctttggtgac tcgcgaaatg aattgctgaa gttgtggctc
     2581 atcttctggc aaaatcgggc ggaacaagca gcgttcaccg tttttcaatt ctacccattc
     2641 ttccagctga tgcggataag ggcgcactgc cagccgactc tcgttatcgc cttcaaacgg
     2701 cgagatatcc agcgtgacat ccagcgcggt aaattcactg ccagaagcca gcaaaggatg
     2761 aatatccaga cgctgaattt ccgggcaatc gacaatcaag ttggaaacct gcaccagaag
     2821 ctggctcaag cctgcaacat ccaatgggcg tagcgcactg cgcgcacgaa tctttttact
     2881 tttgatcccc tgaataacca gatagcgggc caggttcatg ttcagcggcg gcagtgcgac
     2941 gacggcttga tcttcaggac gccactccac accgccttca cccagcatga tcaacggccc
     3001 gaaaaccgga tcgtgcccaa ccacaacccg caactcctga gcgccagcac ggttagccat
     3061 actttgcacc aacaggccgt ggatccgcgc ctgcggccag gccattttta cgcgatcgaa
     3121 aatggcgttt gccgcttgct ggacttcatt ggctgtacgc aagtacagca tgacgccctg
     3181 aacttccgat ttatgtggaa tatccggcga acgcaatttc agcgccaccg gataaccaat
     3241 ctgttcggca atatgcaccg cttcggtgct atcgctggca atccaggtag ggagcgtgtt
     3301 catgccatac gcttgcagga tgggctgaac ttcatgggta tcgagcgacg tagccccttc
     3361 ggcaatcgcc tgttgcaaca gaagatgcgc ttctgcggta ttggaagtca gattgctggg
     3421 caacgccggc gtttcgcgta gttgcttctg attacgccgg tactccacca tatgcataaa
     3481 agcagtgatg gttccttccg gggtacggta ggtcggcagc ccggcttcgc tgaataaacg
     3541 tcgtgcctct tgcgaggagt gctcgccgca ccagttcgtc agcaaagaga catatttgct
     3601 gcggggatga tgctttaccg cttcaattaa tacttgcgcg ctttctgttg cgggagcaga
     3661 ggcgctgggc gaatgaataa ccatcagcgc gtcaaaatcc tggctgtgga gcagaatatc
     3721 cagcgtttta atatagtgct cactgctggc gtcatcgcgt agatcgagcg ggttagatat
     3781 tgccacatgt tctggcagtg catcgcgcag tttctggcag gtttcttcgc ttagcgttgc
     3841 cagcttgcca ttgcgtgacc ataaggcatc cagcgccagc gcggcaggcg cagcaccgtt
     3901 gctgataatc atcagccggt cgccacgtag cgggcgcata tggctaaggg tttccaccgc
     3961 cgaaaacagc tcgtgggtgt cctgtacccg caacaaaccg gcacgctgaa tagccgcatc
     4021 ccatgccggg tccattcctg ccgtggtgtt gagcagtcgc tgtgccgccg ggctacgtcc
     4081 gcttttaatc accagaatcg gtttattacg cgaggcacta cgggccgccg aaacaaagcg
     4141 tcgcgcgtcg cttaactgtt cgagatagag caggatggcg ctggttttac tgtcgcgtgc
     4201 cagatagtca agcaattcat caacgtcgat atccaggctg tcgccgagcg caataaagta
     4261 ggaaaagccc atcttacgct gttgcgccca gtcgaggatg gtgttggaga cggcagccga
     4321 ttgcgaaata aacgccagct tgccgcgttt aatcggcaca ggcgaaaagc tggcattcag
     4381 accttgccag ggagccagta aacccagact gtttggtcca agcaggcgca tgttatggcg
     4441 cagggcgcag gcgcggagat cttcgtgttg cgatgccggg gcggaaagaa taatgcaggt
     4501 tttacagcct ttctcgccga gctcttccag aagagcaaga ttacggctgg cattggtaca
     4561 taaaaccgca aggtcgggtg taaagggcaa gctggcaata tccggccagg ccaacacacc
     4621 caacaccgct ttccaggctg gcgtcaccgg gagtaccggt ccgttaaagc ctcccgccag
     4681 caggttacgc atcatcaggt aacctgcgcg attgggtttc atcgacgcgc caattaccgc
     4741 tatcgatttt ggtcgcagta gtgcttccag tcctcgctga ctcataccgg tctcccgcag
     4801 aagtgaccga atgattttaa acgctttcta actgttctgc tgtgatgcta cccagatgtt
     4861 gcgtttttcc tgccagatag cgtgttttaa agcgggtaaa atgctcgcct aaccctgctg
     4921 ccgccccggt atcgccggcc atatctaaca gtgcgatggc tacctcggca gtacaatatt
     4981 ggccttcagc ctgggcttca cgcaggcgat aggctgaaag ccgggaaaga tcgacggaaa
     5041 tgacgggaag attatccaga tacggacttt tacgaaacat cttgcgagct tccggccagg
     5101 taccatcgag catgataaac agcggtggct taccggcagg tggtgtgaag atcacttccc
     5161 gttgctcatc agcatacgag gcgggaaaga ccaccattgg ctgataatcc gggttttgta
     5221 ccagatccag caaatcctgc gagggttcgg tacgcgacca ttgaaacgca acggtatcag
     5281 gcaaaatatc agcaatcaga cgcccggtat tactgggctt cattggctcg gtgtcgaaca
     5341 tcagcaaaca gaagcgactt tttgcttgtg ctggggtaat tgtcgaacag agacataatt
     5401 tctctggcaa aagacagcgt tggcagcgac gaacgcgatt accgcgggca agaaaaggac
     5461 gtgttgcgcg cgcaatacgc tcggcgcgta actggagaac agcgttttcg gtcataagag
     5521 agcgtcgaaa aaacgccatt gtcgcagagg agaaaacggg gcacaagatg cgccccggta
     5581 agattaaaga gattcgttca gccagctatc gaacggagct ttcggtactg cgccattaag
     5641 catgtcgaca acctgaccgt ttttgaaaat catgatcgtc ggtatactac gaattccaaa
     5701 gcgactgctc aattcacgtt cagcttcggt attcactttc acaaagcgca ctttaccgct
     5761 acgctcttgc gcgacatctt caaaaattgg tgcgaaatta cggcaggggc cgcaccacgg
     5821 tgcccagaag tcgatcacca caggtagatc atccttcagc aatttgtcga gtgtttcacc
     5881 ggtcgcatta atcacctctc cgtcaaacaa gtcgtgaccg cagcgtccgc attttgccgc
     5941 atcttcgatc cgatcgtcgg gaatgcgatt gatggcctga caatgggtac aaacggtatt
     6001 cataactaac ctcgggatga gtaaggagac ttatcggcaa cgacgccggt atgtttctaa
     6061 tatgttacat attatcgggg acattgttcc agacaacaat ctgttttatt caataggtat
     6121 gatagtcgca ggctttggta cgaaatcatg gcgaaccgcg cggacttcgg gtaatctgcg
     6181 cgcttcgcgc agcgctggtg gagaaaagca tgaacgatga aatgaaaggt aaaagcggca
     6241 aggtcaaagt gatgtatgtc cgcagtgatg atgattctga taaacgtacc cacaacccgc
     6301 gtaccgggaa agggggcggg cgtccaggaa aatctcgtgc tgacggtggc cgtcgccccg
     6361 cccgcgatga caaacagagt cagccccgtg accgcaagtg ggaagattcg ccgtggcgca
     6421 cggtttcccg cgcgccgggt gatgagacgc cggaaaaggc cgatcacggt ggcatcagtg
     6481 gtaaaagttt tattgatccg gaagtgttgc gtcgtcagcg tgcggaagaa acccgcgtct
     6541 acggcgaaaa cgcctgtcag gcactgttcc agagccgtcc ggaagcaatt gttcgcgcct
     6601 ggtttatcca gagcgtaacg ccacgtttta aagaagcctt gcgctggatg gcggcaaacc
     6661 gcaaagcgta ccatgtggtg gatgaagctg aattgacaaa agcgtcaggc acggaacatc
     6721 acggcggcgt ttgcttcctg atcaaaaagc gtaacggtac aaccgtgcag cagtgggtaa
     6781 gccaggcagg cgcgcaggat tgcgttctgg cactggaaaa cgaatctaac ccgcataacc
     6841 tgggtggcat gatgcgcagc tgcgcgcact tcggcgtgaa aggtgtcgtg gtgcaggatg
     6901 cggcactgct ggagtcgggg gcggctatcc gtaccgcaga aggcggcgca gagcacgttc
     6961 agccgattac tggcgacaac attgttaacg tgctggatga tttccgtcag gcaggttaca
     7021 ccgtagtgac cacttccagc gagcagggta aaccgctgtt caaaactagt ctgccagcga
     7081 aaatggtact ggtgctgggt caggaatatg aagggttacc ggatgccgca cgcgatccga
     7141 acgatctgcg cgtgaagatt gacggtacgg gcaacgttgc cgggctgaat atctctgtcg
     7201 caaccggcgt tttgcttggt gaatggtggc gtcagaataa agcctgataa atcagccggg
     7261 tggcaactct gccatccggc atttccccgc aaatttactc actctctgcc ggtaatactg
     7321 gcatccagtc aatcggcgtc tcgccacgtt gttccagcca ctgatttgcc agcacaaaat
     7381 ggttgcagcc aaagaatcca cgatgcgccg aaagcggcga cggatgcggt gctttcagta
     7441 catgatggcg ttgcttatct ataatcgccc ctttcttttg cgcatgtgat ccccacaaca
     7501 aaaacaccac gccttcgcgg tgctggttaa tcaggctgat gactttatca gtaaacgtct
     7561 cccaaccgag gctggcgtgg gaatgcgcct gacctgcgcg aaccgtcaac acagtattga
     7621 gcagcagaac gccctgacgc gcccagcttt cgagatagcc atgattaggg cgggtgaagc
     7681 ccggaatagt attttccagc tctttataca tattcaataa tgacggagga atggcaatgc
     7741 cgggacgaac ggaaaatgcc agaccatgcg cctgtctcgg tccgtgataa ggatcctggc
     7801 cgagaatcac cactttaacg tcacccaact cggtaaagcg gaacgcgtta aagacatctt
     7861 tttgtggtgg atagatagtg acgccggact gccgctcgct ggcgacggtc tgaagggtat
     7921 taagaaaata aggttgttgc ttctcttcag ccagcacgtc atgccaggtt aattcgttag
     7981 ccatctcgct ctcctgcgaa tcttcaatcc gcctagctta actgcttctt tctacagaac
     8041 aaaatcatgc tcatttttta gacttaaacg agtgcaaaaa aatgtaaatt ggtaaaattt
     8101 cacacccgca taagttgatg taaaacaata aaatccattt acgaccacaa tcgatgtgtg
     8161 gtttttattg atttaaatca aagaatcaag ggtggttgag gagtatatat acactcaagc
     8221 aacaatggtt ttaccaattg gccgcgacag gctgaacaaa tcaaataatt ttgccgggga
     8281 ggcatcacat gattacaggt atccagatta ctaaagccgc taacgacgat ctgctgaact
     8341 ctttctggct gctggacagc gaaaaaggcg aagcacgttg catcgttgca aaagcaggtt
     8401 atgcagaaga tgaagtggtt gcagtaagca aactgggtga cattgaatac cgtgaagttc
     8461 cagtagaagt gaaaccagaa gttcgcgttg aaggtggtca acacctgaac gttaacgttc
     8521 tgcgtcgtga aactctggaa gatgcagtta agcatccgga aaaatatccg cagctgacca
     8581 tccgtgtatc cggttatgca gttcgcttta actctctgac tccggaacag cagcgcgacg
     8641 ttatcgctcg tacctttact gaaagcctgt aaggcttaag tagcaagtta ctaagcggaa
     8701 gagggcgacc tcttccgctt tttttcgtta atagaaaatg cgtaccgcgc aatagaccag
     8761 caacagggca agcacgatat ttaactggcg accatactgg cgaaacaatc gctgaaacag
     8821 atgccccgcc agcgcccagc acacattgcc aaacgtccca atcatcgcca gcaaaacgct
     8881 gacgccaact acccagctta gcgcctgtgt ttgcggcaga acaaacgtcg acagtgccgt
     8941 aacaccgtac aaaatgattt tgacgttcac aaactgcaaa gcaaagctgg cccaaaagct
     9001 gattggtttt gcctgaagtc cgtcttcctt tgttgggctg gtggcgattt tccacgccag
     9061 ccagacaata tatgccgccc ccgcccaact caaaaggtgt accgctgccg ggtcaatcac
     9121 tgccagtgaa aatgaaatgc ccgcacacag taacatcaca atcaaaaatc ccagactcat
     9181 ccctgccagc acgcgggtac tttgacgaaa tccatgcgac gtagcagagc taagggcgag
     9241 aatattgttc ggtcctggcg tcatagcggt aatcagggtg taagtccaaa aagcacttaa
     9301 aagggtcggt gtcacttgtt atctcctctg ttttggtaga cagta
//
LOCUS       DAJTMK010000147         9215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTMK010000147 DAJTMK010000000
VERSION     DAJTMK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.150"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..303
                     /locus_tag="OO135_003205"
     CDS             <1..303
                     /locus_tag="OO135_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5398954.1"
                     /translation="FVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLY
                     KLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            355..1511
                     /locus_tag="OO135_003206"
                     /pseudo
     CDS             355..1511
                     /locus_tag="OO135_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(1477..1599)
                     /locus_tag="OO135_003207"
     CDS             complement(1477..1599)
                     /locus_tag="OO135_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5398955.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            1802..3487
                     /locus_tag="OO135_003208"
     CDS             1802..3487
                     /locus_tag="OO135_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR5398956.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            3525..5897
                     /locus_tag="OO135_003209"
     CDS             3525..5897
                     /locus_tag="OO135_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5398957.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVINTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKIR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5954..8737
                     /locus_tag="OO135_003210"
     CDS             5954..8737
                     /locus_tag="OO135_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5398958.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMLDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQERLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         2720 a         2003 c         2006 g         2486 t
ORIGIN      
        1 tttgttcgcc atcagtcaga cgattacccg cataacccca acactgagga cttaagccaa
       61 ttctcttata cggtgagaaa taacgattat tcgcttgtct atacagtaga aaacaatcag
      121 ttaggtctct acaaactgac ggatctacag caaaaagata accttgccgc cgccaatccg
      181 caggtcgtta aagagatgca aggcgtggta agagagttta tcgacagcag ccagccaccg
      241 cttagcgagg taaatcagga gaagtttaac aatatcaaga aagcactaag cgaagcgaaa
      301 taactaaacc ttcatgcggc ggaatttttc gccgccttat tgagcgagat agcgatgcac
      361 gttacagcca agccctccag ttttcaatgt aatctcaaat gtgattactg tttttacctt
      421 gaaaaagagt cgcagtttac tcatgaaaaa tggatggatg acagcactct gaaagagttc
      481 atcaaacaat atatcgcagc gtctggcaat caggtctatt ttacctggca aggcggtgaa
      541 tccactctgg ctggcctgga ttttttccgt aaagttattc actatcaaca acgctatgca
      601 ggccaaaaac gtatttttaa tgcattacaa acgaatggca ttttattgaa taatgaatgg
      661 tgttcctttc tcaaagaaca tgaatttctg gttggtatct cgatcgatgg cccccaggag
      721 ttacatgacc gttacagacg cagtaattca ggtaacggta cttttgcaaa agtgatagca
      781 gccatcgagc gtctgaaatc atatcaagtt gagtttaata cgttaccgtc attaataacg
      841 ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata
      901 tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt ggtcatagtg
      961 agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt
     1021 caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatt cgtcagtttg
     1081 aaagctatgt cagccgtttt ttggggaatg ggcatacaag ttgtattttc caggagtcct
     1141 gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct
     1201 atccacagta caaaattgga aacattaata aatctgaact caaaacgatg aacagtgtac
     1261 aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     1321 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     1381 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     1441 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     1501 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     1561 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     1621 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     1681 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     1741 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     1801 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     1861 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     1921 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     1981 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     2041 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     2101 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     2161 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     2221 cgctgaagac attctcttac tgatcagcaa aacgctcagt ctttcatttg gcttcatcca
     2281 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     2341 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     2401 cattgtgatc ggtggaactt tatttaccca taaggttggt aagcgtattc gtccgcttaa
     2461 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     2521 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     2581 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     2641 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     2701 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     2761 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     2821 ggttatcgat cgcttgtatg agttccatca actcactgaa cagcgcccta cgaataagcc
     2881 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     2941 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     3001 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtggtt
     3061 taaaggtgat attttttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     3121 aaccggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     3181 gttgagcgaa gtactgcatc aggttggtct tgggaaattg gctgcgcgta ttcatgacca
     3241 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     3301 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     3361 agaggctatc cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     3421 ggttacacat caacccggtg tctggaacct ggccgatgat atttgtgaca ttagcgcggt
     3481 tttataaaaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     3541 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
     3601 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
     3661 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
     3721 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
     3781 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
     3841 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
     3901 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
     3961 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
     4021 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
     4081 gcactacgcg ttcggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
     4141 atcaaggttc ttcaggaagt acttatttct ctccagattt taaaaagaac ttttatacct
     4201 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
     4261 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
     4321 agtataaaaa cgttatcaat actgcattga gcaaatttac ctggtttgcc agcgaccgct
     4381 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagtacct
     4441 tcccgcagtc tgatcgcgaa atgggtaata aatcctatgg tcttgcatgg gatatggata
     4501 cgcaactcgc atgggccaaa atacgtacca ccgttggttg ggatcatatt agtgattata
     4561 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
     4621 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
     4681 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctactccg
     4741 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
     4801 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
     4861 taggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
     4921 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
     4981 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
     5041 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
     5101 cggaatcggt atcaggtaat aaaaccctga cgcgctatca gaatttgaaa acgccttata
     5161 acgatgaact ggcaatggga ttgcagcaaa aaatcggtaa gaacgttatt gcacgcgcaa
     5221 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
     5281 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
     5341 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
     5401 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
     5461 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
     5521 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
     5581 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
     5641 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
     5701 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
     5761 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
     5821 ttgatacaac gaataccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
     5881 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
     5941 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
     6001 ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa aagttaatta ccgggcaact
     6061 ggacaatggc ttgcgatata tgatttatcc gcatgctcaa ccaaaggatc aggtaaattt
     6121 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
     6181 ttttgtagaa catatgatgt ttaacggcac aaaaacatgg ccgggtaata aagtcatcga
     6241 aacatttgag tcaatgggcc tgcgttttgg tcgcgatgtt aatgcctata ccagctatga
     6301 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
     6361 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
     6421 acgtggcgta attactgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
     6481 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
     6541 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
     6601 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
     6661 gctgataaag gataatttaa gtaagcttcc ggctaacaaa gcagctgaaa atcgcgtctg
     6721 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
     6781 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
     6841 tatcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
     6901 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
     6961 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgctggatgc
     7021 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
     7081 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
     7141 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
     7201 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
     7261 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
     7321 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
     7381 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
     7441 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
     7501 tctgacatca ttaacacttt ccaatggtgc cagggttatt ctggcaaaat ccgcgggtga
     7561 agagcaaaag ctacaaatta ctgccgtatc gaataaaggc gatttaagtt tccctgcgca
     7621 gcaaaaatca cttatcgcgc tggcaaataa agcagtaagc ggaagcggcg ttggtgagct
     7681 ctcctcttcc agcctgaaac gctggagtgc ggaaaattcg gtaaccatga gcagtaaagt
     7741 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
     7801 tcagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
     7861 acaaaatgct caaattcagg cattgaaaac gctcgaccag cgtccagcgg agaaattcgc
     7921 ccagcagatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
     7981 gattgtacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
     8041 agcggatatc acgtttgtca ttgtcggtaa tgtctcagaa gacaaactcg tggcgttaat
     8101 tacgcgttac ttaggatcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
     8161 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
     8221 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
     8281 gctcgatgct tttaacgtcg cactggcaaa agatctacgt gttaatattc gtgaacaggc
     8341 atctggagca tacagcgttt cttctcgcct ctcggttgat cctcaggcca aagatatcag
     8401 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaactgttaa cgttagcgaa
     8461 tgaagtgatg gttaagcgtc tggctaaagg gatcagtgag caagaactga atgaatatca
     8521 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
     8581 tattgtaaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcgatt
     8641 gttgaaacaa atgacggtag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
     8701 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
     8761 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
     8821 ataataatca agtactaata gtgatatttt taagtctgat ttttacgtga taattcagga
     8881 gacacagaat gcgcataaaa ataacagcat aaaacacctt accaccaccc aagaatttca
     8941 tattgtattg tttttcaatg aaaaaaacat tattcgcgta atatctcacg ataaataaca
     9001 ttaggatttt gttatttaaa cacgagtcct ttgcacttgc ttactttatc gataaatcct
     9061 acttttttaa tgcgatccaa tcattttaag gagtttaaaa tggataagaa gcaagtaacg
     9121 gatttaaggt cggaactact cgattcacgt tttggtgcga agtctatttc caccatcgca
     9181 gaatcaaaac gttttccgct gcacgaaatg cgcga
//
LOCUS       DAJTMK010000148         9133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTMK010000148 DAJTMK010000000
VERSION     DAJTMK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9133
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.151"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            143..2012
                     /gene="kup"
                     /locus_tag="OO135_003211"
                     /pseudo
     CDS             143..2012
                     /gene="kup"
                     /locus_tag="OO135_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
     gene            2179..2598
                     /gene="rbsD"
                     /locus_tag="OO135_003212"
     CDS             2179..2598
                     /gene="rbsD"
                     /locus_tag="OO135_003212"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5398959.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2606..2974
                     /locus_tag="OO135_003213"
     CDS             2606..2974
                     /locus_tag="OO135_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein"
                     /protein_id="HCR5398960.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGNT
                     RGVDVGAKKEIYQLINQFNADGLSIILVSSEMPEVLGMSDRIIVMHEGHLSGEFTREQ
                     ATQEVLMAAAVGKLNRVNQE"
     gene            2979..3944
                     /gene="rbsC"
                     /locus_tag="OO135_003214"
     CDS             2979..3944
                     /gene="rbsC"
                     /locus_tag="OO135_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5398961.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQNSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            3969..4855
                     /gene="rbsB"
                     /locus_tag="OO135_003215"
                     /pseudo
     CDS             3969..4855
                     /gene="rbsB"
                     /locus_tag="OO135_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            4981..5910
                     /gene="rbsK"
                     /locus_tag="OO135_003216"
     CDS             4981..5910
                     /gene="rbsK"
                     /locus_tag="OO135_003216"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5398962.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            5914..6906
                     /gene="rbsR"
                     /locus_tag="OO135_003217"
     CDS             5914..6906
                     /gene="rbsR"
                     /locus_tag="OO135_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5398963.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(6872..8299)
                     /gene="mdtD"
                     /locus_tag="OO135_003218"
     CDS             complement(6872..8299)
                     /gene="mdtD"
                     /locus_tag="OO135_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5398964.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPTIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSMGIVGNIATRL
                     GTGSVQFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(8322..9014)
                     /locus_tag="OO135_003219"
     CDS             complement(8322..9014)
                     /locus_tag="OO135_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5398965.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2219 a         2203 c         2413 g         2298 t
ORIGIN      
        1 caaagggggg tatttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
       61 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
      121 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
      181 cctcgcggct attggagttg tctacggcga tattggtacc agcccgttat atacacattc
      241 gtgaatgttt gtccggccag tttggttttg gcgttgaacg cgatgccgtg tttggctttt
      301 tatcgctgat cttctggctg ctaatctttg tggtttccat taaatatctc accttcgtga
      361 tgcgggcaga taacgccggt gaggggggga tcctgacgtt gatgtcgctt gccgggcgta
      421 atacgtcggc gcgaaccaca tcaatgctgg tgattatggg gctaatcggc ggcagctttt
      481 tctatggtga agtcgtcata acacccgcta tttcggtgat gtcagccatt gaaggtctgg
      541 aaatcgtcgc cccgcagctg gatacctgga tagttcccct ctcaattatc gttctcactt
      601 tattatttat gattcaaaaa catggcacgg ctatggtcgg taagctgttt gcgccgatca
      661 tgctgacctg gtttttgatt ctggcaggac tggggttacg tagcattatt gctaacccgg
      721 aagtgctgca tgcgctgaat ccaatgtggg cggtgcattt cttccttgaa tacaaaacgg
      781 tttcttttat tgcattaggg gcagtggtgt tgtcgattac ggggggcgag gcgctgtatg
      841 ctgatatggg gcactttggt aagttcccta ttcgcctggc gtggttcacc gtcgtattac
      901 cttccttgac ccttaattac ttcggtcagg gagcgctgtt gttaaagaac ccggaagcga
      961 ttaagaaccc gttcttcctg ttggcaccgg actgggcgct gatcccgctg ctgatcatcg
     1021 ccgcactggc gacggtaatt gcctcgcagg cggttatctc tggcgtcttc tcattgacgc
     1081 gtcaggcggt acgtctggga tatttgtcgc cgatgcgcat tattcacacc tccgaaatgg
     1141 agtcagggca aatctatatt ccgtttgtga actggatgct ctatgtcgcg gtcgtgattg
     1201 tgattgtcag ctttgagcac tccagcaacc tggcggcggc gtacgggatt gcggtgaccg
     1261 gaaccatggt gctgacgtct attctctcga ctaccgtggc acgtcagaac tggcactgga
     1321 ataagtattt tgttgcgctg atcctgattg ctttcctttg tgtcgatatt ccattgttca
     1381 ccgctaacct cgataaactg ctctccggcg gttggttgcc attgagcctc ggtacggtga
     1441 tgtttatcgt gatgaccacc tggaagagcg agcgtttccg cttgctgcgg cggatgcatg
     1501 aacatggtaa ctctctggaa gcgatgattg cttcgctgga gaaatcaccg cctgttcgcg
     1561 tgcccgggac cgcggtgtat atgtcgcgtg caatcaacgt cattcccttt gcgctgatgc
     1621 ataaccttaa acataacaag gtattgcatg agcgggtgat tctgttaact ctgcgtaccg
     1681 aagacgcgcc atatgtccat aatgtccgtc gggtacagat tgaacaactg tcgctcactt
     1741 tctggcgcgt ggtggcaagt tatggttggc gagaaacgcc aaacgtggaa gaagttttcc
     1801 accgctgcgg tctggaagga ttaagttgcc ggatgatgga aacctccttc tttatgtcgc
     1861 atgagtcgtt gatcctcggc aaacgcccgt ggtatttgcg tctgcgcggc aagctgtact
     1921 tgctgctgca acgtaatgcg ctgcgtgcac cagatcaatt tgaaatcccg ccaaacaggg
     1981 ttatcgaact gggtactcag gtcgaaatct aacgccagac gcctcctttc tccataaggg
     2041 ggcgtttttg ttttcatggt taatcaccat gtaaaacgtt tcgaggttga tcacatttcc
     2101 gtaacgtcac gatggttttc ccaactcagt caggattaaa ctgcgggtca gcgaaacgtt
     2161 tcgctgatgg agaaaaaaat gaaaaaaggc accgttctta attctgatat ttcatcggtg
     2221 atctcccgtc tgggacatac cgatacgctg gtggtgtgtg atgctggttt acccatcccc
     2281 aaaagtacaa cgcgtatcga tatggcatta acccagggtg taccttcttt tatgcaggtg
     2341 ctgggagtcg ttacaaatga aatgcaggtc gaggctgcca ttatcgcgga agagatcaaa
     2401 caacataatc cgcaactcca cgaaacgttg ctcactcacc ttgagcagct gcaaaaacac
     2461 cagggaaata ccattgaaat tcgttacacc acgcatgaac aattcaaaca acaaaccgca
     2521 gaaagtcagg cggtaattcg cagcggagaa tgttctccgt atgcgaatat cattctctgt
     2581 gctggcgtga cgttctgagg ccgtcatgga agcattactt cagcttaaag gcatcgataa
     2641 agccttcccg ggcgtaaaag ccctctcggg cgctgcgtta aatgtctatc cgggccgcgt
     2701 gatggcgctg gtgggcgaaa acggcgcggg taatacccgt ggcgtagatg tcggcgcgaa
     2761 aaaagagatc tatcaactga ttaaccagtt caacgccgat ggcttgagca tcattctggt
     2821 gtcatcggag atgccagaag tattaggcat gagcgatcgc atcatcgtca tgcatgaagg
     2881 gcatctcagc ggggaattta ctcgtgagca ggccacccag gaagtgttaa tggctgccgc
     2941 tgtgggcaag cttaatcgcg tgaatcagga gtaaaaaaat gacaacccag actgtctctg
     3001 gtcgccgtta tttcacgaaa gcgtggctga tggagcagaa atcgcttatc gctctgctgg
     3061 tgctgatcgc gattgtctcg acgttaagcc cgaacttttt caccatcaat aacttattca
     3121 atattctcca gcaaaactcg gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc
     3181 tgacgtcggg catcgacttg tctgtaggtt ctctgttggc gctaaccggc gcagttgctg
     3241 catctatcgt cggcattgaa gtcaatgcgc tggtggctgt cgctgctgct ctcgcgttag
     3301 gtgccgcaat tggtgcggta accggggtga ttgtagcgaa aggtcgcgtt caggcgttta
     3361 tcgctacgct ggttatgatg cttttactgc gcggcgtgac catggtttat accaacggta
     3421 gcccagtgaa taccggcttt actgagaacg ccgatctgtt tggctggttt ggtattggtc
     3481 gtccgctggg cgtaccgacg ccagtctgga tcatggggat tgtcttcctc gcggcctggt
     3541 acatgctgca tcacacgcgt ctggggcgtt acatctacgc gctgggcggc aacgaagcgg
     3601 caacgcgtct ttctggtatc aacgtcaata aaatcaaaat catcgtctat tctctttgtg
     3661 gtctgctggc atcgctggcc gggaccattg aagtggcgcg tctctcctcc gcacaaccca
     3721 cggcggggac tggctatgag ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc
     3781 tggcgggcgg aaaaggtcgc attgttggga cgttgatcgg cgcattaatt cttggcttcc
     3841 ttaataatgg attgaatttg ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg
     3901 tggtgatttt gctggcggtg ctggtagaca acaaaaagca gtaataacga ctacaggaca
     3961 tcttgaatat gaacatgaaa aaactggcta ccctggtttc cgctgttgcg ctaagcgcca
     4021 ccgtcagtgc gaatgcgatg gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca
     4081 acccgttctt tgtatcgctg aaagatggcg cgcagaaaga ggcggataag cttggctata
     4141 acctggtggt gctggactcc cagaacaacc cggcgaaaga gctggcgaac gtgcaggact
     4201 taaccgttcg cggcaccaaa attctgctga ttaacccgac cgactccgac gcagtaggta
     4261 atgctgtgaa gatggctaac caggcgaaca tcccggttat cactcttgac cgccaggcaa
     4321 cgaaaggtga agtggtgagc cacattgctt ctgataacgt actgggtggc aaaatcgccg
     4381 gtgattacat cgcgaagaaa gcgggtgaag gtgccaaagt tatcgagctg caaggcattg
     4441 ctggtacatc cgcagcccgt gaacgtggcg aaggcttcca gcaggccgtt gctgctcaca
     4501 agtttaatgt tcttgccagc cagcagattt tgatcgcact aaaggtttga acgtaatgca
     4561 gaacctgttg accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc
     4621 gctgggcgcg ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt
     4681 tgacggtaca ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc
     4741 tcagctaccc gatcagattg gcgcgaaagg cgtcgaaacc gcagataaag tgctgaaagg
     4801 cgagaaagtt caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta
     4861 atcaggttgt atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat
     4921 aaagaaaagc agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat
     4981 atgcaaaacg caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat
     5041 cttcaatctt ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt
     5101 ggcggcaaag gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt
     5161 attgcctgta cgggtgatga cagcattggt gagagcgttc gccagcagct cgccactgat
     5221 aacattgata ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt
     5281 tttgttaatg gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgccctt
     5341 tccccggcgc tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc attattaatg
     5401 cagctggaat caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag
     5461 actatcgttg cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg
     5521 gtggacatta ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa
     5581 aatgatgaag atgcagcgaa ggcggcgcag gtactgcatg aaaaaggtat ccgtactgta
     5641 ctgattactt taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt
     5701 cctggattcc gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg
     5761 ttaatcacgg cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct
     5821 gcagctgcga ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag
     5881 atcgacgcat ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgc
     5941 ctggcgggcg tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt
     6001 gaagcgatta ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagcc
     6061 ctggcgcgta gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt
     6121 accaatcctt tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt
     6181 tatagtctcg tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa
     6241 acgctgatgc aaaaacgcgt tgatggcttg ctgttactgt gcaccgaaac gcatcaacct
     6301 tcgcgtgaaa tcatgcaacg ttatccgaca gtgcctactg tgatgatgga ctgggctccg
     6361 ttcgatggcg acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg
     6421 caatatctga tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa
     6481 actccggcgc gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac
     6541 attcctgatg gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg
     6601 cgccaactgt tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg
     6661 gctgttggcg tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg
     6721 gtgattggct atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc
     6781 caccaaccga aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc
     6841 cagccgaccc ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg
     6901 gcttagattt acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga
     6961 acattgctgc tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt
     7021 cgacagtcgt tgtgccttcc atcccttcat aaacgcgaag gacggccgca cttacagcaa
     7081 cgcctaaact aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt
     7141 tgtcatcggt cagatcggca agtgtgatgg tattcatcgc ggtaaattgc gtcgacatag
     7201 ccatccctaa tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca
     7261 aagagaactg agcgatcatt agcccaataa tcaccgtgat ccccactaac gtatggcgat
     7321 agcccagacg acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg
     7381 tcggtgccat catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca
     7441 atggcataag gaactgtaca ctaccggtcc ccagacgggt tgcaatattg cctacgatac
     7501 ccatcgagaa agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtgtgtc
     7561 gcgcatggag aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa
     7621 tccagctggc gacaatcttt tccccgaata gctctattcc gcttgagaag agaacaaggc
     7681 tgaggccaaa cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg
     7741 gcatatgttt gcgcgcgtaa agaaggcccg caatacctat ggggatattg attaaaaata
     7801 tccagtgcca ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc
     7861 ccaccagacc cggcatggcg acaaaattca atacaggaag aagttcatta cgaggataag
     7921 cacgcagtaa ggccagccga gcaacaggca tcatcattgc gccgcctatc ccctgaataa
     7981 cccggaagac aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg
     8041 tgaacagact cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc
     8101 cgcttaccgg aataaacatc gccaccgtca gcgtataact gatgatggct gattgcatcg
     8161 cgagaggaga acgattaagg ctatgagcga ttgtgggtaa ggcggtattc agaatagtgg
     8221 catcaagtgc ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gccatactac
     8281 gcttcttttt atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt
     8341 agcagcgcct gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca
     8401 atcgcctggt gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg
     8461 tgataaaccg aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca
     8521 tagatatgct catgccaggc catatcgact tcgatccagc gttcacggcg aaagttcttt
     8581 tttaatgccg ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca
     8641 accgttgctg ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga
     8701 tcgatgacct gatgaaagtt ctcttctgtc atccaccagg taagcaattc ctgatcaaga
     8761 aaattccagt ttgattgtgg catgacccgg gtaccaattc gcggtcgcgg taaaaccatc
     8821 ccttttgccg ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc
     8881 tcgcccagct caatctcacc aggcaaaatg gtgccgggtt catattcacc ttttaagatg
     8941 cgttgcgcca gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt
     9001 gcgcttaaag gcattactaa tcttcctttt tctttttatt cctccttagt atgccaccag
     9061 gaagtgtgat tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggc
     9121 ttttgctgaa aaa
//
LOCUS       DAJTMK010000149         9009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTMK010000149 DAJTMK010000000
VERSION     DAJTMK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9009
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.152"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..574
                     /locus_tag="OO135_003220"
                     /pseudo
     CDS             1..574
                     /locus_tag="OO135_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            884..1612
                     /gene="mpaA"
                     /locus_tag="OO135_003221"
     CDS             884..1612
                     /gene="mpaA"
                     /locus_tag="OO135_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5398966.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1587..2552)
                     /gene="ycjG"
                     /locus_tag="OO135_003222"
     CDS             complement(1587..2552)
                     /gene="ycjG"
                     /locus_tag="OO135_003222"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5398967.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2671..3177
                     /gene="tpx"
                     /locus_tag="OO135_003223"
     CDS             2671..3177
                     /gene="tpx"
                     /locus_tag="OO135_003223"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5398968.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNSEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3221..4762)
                     /gene="tyrR"
                     /locus_tag="OO135_003224"
     CDS             complement(3221..4762)
                     /gene="tyrR"
                     /locus_tag="OO135_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5398969.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDAIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVSRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4910..5971)
                     /locus_tag="OO135_003225"
     CDS             complement(4910..5971)
                     /locus_tag="OO135_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5398970.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5968..7365)
                     /locus_tag="OO135_003226"
     CDS             complement(5968..7365)
                     /locus_tag="OO135_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5398971.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGVPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7520..8518
                     /locus_tag="OO135_003227"
     CDS             7520..8518
                     /locus_tag="OO135_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5398972.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8629..>9009)
                     /gene="ompG"
                     /locus_tag="OO135_003228"
     CDS             complement(8629..>9009)
                     /gene="ompG"
                     /locus_tag="OO135_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR5398973.1"
                     /translation="TGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLP
                     LTLGNHSVTPYTRIGLDRWSNWDWQDDIECEGHDFNRVGLFYGYDFQNGLSVSLEYAF
                     EWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         2262 a         2428 c         2154 g         2165 t
ORIGIN      
        1 agataaacaa accattaaac aaactttatt tgcccgtcag ccagcaaaaa tccacaaacc
       61 ctatccaact aatagcaaga ttattatggg agatgtactg aatcatgcag cactgaagca
      121 ggccatgcag ggacaggacg ttgtgtatgc caaccttacg ggggaagatt tagatattca
      181 ggcaaattca gtgatcgctg cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct
      241 ttccctcggt atttatgatg aagttccagg aaaatttggc gaatggaata atgcagttat
      301 tggtgagccc ctaaaaccat tccgcagggc tgcggatgca attgaggctt ctggactgga
      361 atatacaatt cttcgccctg cctagctgac tgatgaagat attattgatt atgagcttac
      421 ctcacgcaat gaaccattca aaggtacgat cgtatcacgt aagagtgtcg cagcattaat
      481 cacagatatc atcgataaac ctgagaaaca cattggtgag aatataggta tcaatcaacc
      541 cgtgactgat ggtgacaagc cattattcat gtaaatcaat tacatagtta ccatcgtaat
      601 gtactatgtg cagtacaatt tattaaactt attaaggtga actgatcaat ccgatgcgat
      661 taacatgtgt agaaccagta agaataaaat acgccatgta tataacatta cgacttacat
      721 ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac ttccggcaaa
      781 aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa tccgcaaaat
      841 ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg taacccgccc
      901 acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt cattattggg
      961 cgcgccgtta atctggtttc ctgcccctgc agccagccgc gaaagtggtt tgattctggc
     1021 tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc tgcggacgtt
     1081 gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg gctgccagct
     1141 ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg cggcgaactg
     1201 gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg atgtcgtatt
     1261 actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc agcttataca
     1321 ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta ttgaagatcc
     1381 cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc tggtaaccag
     1441 cgtcggttat gaaacgcctg gctctttcgg cagttggtgt gcagatctca acttgcattg
     1501 catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat atctctttgc
     1561 gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt gaactgaagc
     1621 gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatctgcgaa actgacctgc
     1681 ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca gcccagcatc
     1741 agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt cagaccaccg
     1801 gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt cagattgcta
     1861 cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt ctccagcgcc
     1921 gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc cgccaatagc
     1981 tggcaacgcg ccgccaaccc ttctgcacgc caggattcat ttgcatcaac gatcagcgtc
     2041 gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg gttatccagc
     2101 ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt ggccatctga
     2161 tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag cgttatgccg
     2221 atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa tgcacaatcc
     2281 agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg tgtcagccct
     2341 ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc actttctcca
     2401 taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc cagttcaacc
     2461 accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt atgtaagggc
     2521 caggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg cggtggtctg
     2581 cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac taggtgtgac
     2641 gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt ccagggcaac
     2701 ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac ttttactctc
     2761 gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg caaagtgctg
     2821 aacattttcc cgagtattga taccggtgtt tgcgccgcat cagtacgtaa gtttaaccaa
     2881 ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct gccgttcgcc
     2941 cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc cactttccgt
     3001 aactctgaat ttctgcaagc cttcggtgtg gcaattgctg atggcccact gaaaggtctg
     3061 gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag ccagctggtg
     3121 gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa agcataagtt
     3181 aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg ttcttcttct
     3241 ggcttaagcc atattcccgc aacttattgg caatcgcggt atgtgaaacg ccgagacgtt
     3301 ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc gagcgttcaa
     3361 aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg gctaccgttg
     3421 cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg tccagttgtg
     3481 tcagtgcgcg atagatagcg ttctttaact gccgcacatt tcccggccac gcataacgcg
     3541 taagtacagt attcaggtca gcggccagtt tcggacgcga cacgccctgc tcgtcggcaa
     3601 agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca cgtagcggcg
     3661 gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac atgccttttt
     3721 gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg acatgcacct
     3781 catggtcttc gccaacccga cggaaagtgc catcattaag aaaacgcagt aatttcgcct
     3841 gcatccgtgg tgacatttcc cctattgcat ccaacagcac cgaaccaccg ttcgcctgct
     3901 caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg accgcatctt
     3961 ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg cttgcctgat
     4021 ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc agcagcggcg
     4081 cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc gggctgacgg
     4141 cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac tggcggccca
     4201 tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga tcattttcat
     4261 cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata acgacatgct
     4321 cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag ccgttaatca
     4381 attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc tgacagctcg
     4441 ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt tcgggcaacg
     4501 cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac gggacagtac
     4561 gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg aaactctcaa
     4621 actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca ataccgcgta
     4681 aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg agtcggtctt
     4741 cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat taatatgatg
     4801 atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca caaagacgga
     4861 aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat tatttttcgc
     4921 tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga cgacggaaat
     4981 ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc tccatagctt
     5041 taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg cgggtagaca
     5101 aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc agttcgctgg
     5161 ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg ctgtaatacc
     5221 ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg cgccaggcga
     5281 taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt gattccgccg
     5341 ccgaacggct gatttcgcgc cgcgcctggg catctaaaac tggctggacc aaatgcgcat
     5401 acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac cagcgttgca
     5461 gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa aatgcgcggc
     5521 ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca tgggcgcgct
     5581 gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca gcgccgataa
     5641 tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc caggcattca
     5701 ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc ccgcccatca
     5761 ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc gcttcaactt
     5821 gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc gcctgatttt
     5881 cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc ggaccgtcga
     5941 aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta aaaattccag
     6001 cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct gcgggcgaaa
     6061 cgcttcaaac tggaaccctt gtttgtccca gaacgcctga ccgggcaaac gcgcaggaac
     6121 ttcaccggga taaacggtga gtggcacacc atcgctgagc cggttaccgc gcaatgccgg
     6181 aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg aggccagccc
     6241 caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct gaatcagttg
     6301 ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg ctttagtggc
     6361 agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg tacgctgccc
     6421 gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa atgcctgtgg
     6481 cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa agcgcagaaa
     6541 atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc gctcgcgcag
     6601 cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc aggtatcgac
     6661 atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcctg gcaagacaaa
     6721 gcgccctggc tgaataaagt gcagcccctg ctgtttacag tggtggagat aatcggtcca
     6781 cgcggcggca atgtctgcca gccggttttc gtcggcaggt gctagcgggt ccagcccttc
     6841 gcacatcatt cgccatttga ccgaccattc gccgcgctga ccattgagta agcccgtcat
     6901 ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca accattcgcc
     6961 gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc gtagcagcga
     7021 atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg ttggcgtcgg
     7081 ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga agcgcggaat
     7141 gccaaagtca cgctgaggaa tgcgttttac gcccagcagg cgctcttcac gcaccgcgct
     7201 taacagcggc aagcgtgctc cggcgtgaat attgagcaac tgattgacca tcgcagtgat
     7261 aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg aggcgcagat gtctgtcgac
     7321 accccgattc accagcgcat taagttcatt tttaagtcgc ttcattgctg tcctgtgctg
     7381 ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat gtgggggcca ggccattgat
     7441 tatcaactgc ccgtgttgtc atcgacgggc gaactccata agatgtgcag cattcacgtt
     7501 cggacggagt tcctgaccta tgtcccctac tatttatgat attgccaggg ttgcaggcgt
     7561 atcaaaatcc accgtatcac gcgtgctgaa taagcaaact aatatctccc cggaagcgcg
     7621 cgaaaaagtg ttacgggcca ttgaagaatt acagtatcag ccaaacaagc tggcccgcgc
     7681 gctgacctct tcgggttttg atgccattat ggtgatttct acccgttcga ccaaaactac
     7741 ggcgggtaat ccgtttttct ctgaagtttt gcatgccatc accgccaaag ctgaagagga
     7801 aggtttcgac gtgatattgc agacgtcgca caatccggca gaagacttac aaaaatgtga
     7861 aagcaaaatt aagcagaaaa tgattaaagg cattattatg ctaagttctc cagcggatga
     7921 gtcatttttt gcccaacttg ataaatacga tattcctgtc gtggtgattg gcaaagttga
     7981 aggtcaatat gcccatgttt attctgtcga taccgataat tttggcgaca gcattgcgtt
     8041 gaccgatgca ttaattgaaa gtgggcatca aaatattgcc tgcctgcatg ctccgcttga
     8101 tgtccatgtt tcagtggatc gggtaaatgg ttataagcag agcctggctg cgcataatat
     8161 tgcagtgcgt gatgaatgga ttgttgacgg tggttatacc catgaaacag cactgcaagc
     8221 cgcgagagaa ttattaagcc agtcaccgtt gcctgaggcg gtgtttgcca ctgacagtct
     8281 gaaattaatg agtatttatc gtgcggcggc agagaaaaat attgctattc cgcagcagtt
     8341 agcggtggtg ggttatagta atgaaacgct gtcgtttatt ttaacacctg caccgggcgg
     8401 catcgatgtt ccgacgcagg agttaggtca acaaagctgc gagttattat tccgcttaat
     8461 ttctggaaaa ccgtcaccac agaatattac cgttgccacg catatgacgc tgaaataaat
     8521 accagctacc ttgcctgaat gccggatgcg atgctagcgc atcttatccg gcctacattt
     8581 atagatcgga taaagtactc gcgccgcatc cggcaattta gcccattatc agaacgagta
     8641 atttacgccg acacctgcat aatggaattt atcactgtcg ccttcgtcgt gatcctgcca
     8701 ctcaaacgcg tattccagcg aaacggaaag tccgttctgg aaatcataac cgtaaaataa
     8761 acctacacgg ttaaaatcat ggccttcaca ttcaatatca tcctgccagt cccagttact
     8821 ccagcgatcc agcccaatgc gcgtatacgg cgtcaccgag tggttgccga gcgtcagcgg
     8881 caaataggcg cgaatttctt gcgtggaaaa ctcaccgtta ttgcggctgt cgtccatatt
     8941 gaagccgcgc tcgagataat agttcactcg caaggcaacc gtttcgttga aggtatattg
     9001 cagacctgt
//
LOCUS       DAJTMK010000150         8891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTMK010000150 DAJTMK010000000
VERSION     DAJTMK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.153"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..927
                     /locus_tag="OO135_003229"
                     /pseudo
     CDS             1..927
                     /locus_tag="OO135_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
     gene            complement(1037..2080)
                     /locus_tag="OO135_003230"
     CDS             complement(1037..2080)
                     /locus_tag="OO135_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5398974.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(2206..2781)
                     /gene="nudK"
                     /locus_tag="OO135_003231"
     CDS             complement(2206..2781)
                     /gene="nudK"
                     /locus_tag="OO135_003231"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5398975.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLID"
     gene            complement(2849..4828)
                     /gene="aegA"
                     /locus_tag="OO135_003232"
     CDS             complement(2849..4828)
                     /gene="aegA"
                     /locus_tag="OO135_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5398976.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            5034..6734
                     /gene="narQ"
                     /locus_tag="OO135_003233"
     CDS             5034..6734
                     /gene="narQ"
                     /locus_tag="OO135_003233"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR5398977.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            6898..8891
                     /locus_tag="OO135_003234"
                     /pseudo
     CDS             6898..8891
                     /locus_tag="OO135_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT         2050 a         2383 c         2327 g         2131 t
ORIGIN      
        1 ctcacggaaa gtcatactgc cttacgggcc gatgctgcct gagttgctcg gcggttcggc
       61 ggatctggct ccagcaacct gaccatctgg aaaggttctg tttcgctgaa ggaagatccg
      121 gcgggcaact acattcacta cggggtgcgt gaatttggca tgaccgctat agccaacggc
      181 atcgcgcacc acggcggctt tgtgccgtat accgcgacgt tcctgatgtt tgttgaatac
      241 gcccgtaacg cctcgcggat ggcggcactg atgaaagcgc ggcagattat ggtttatacc
      301 cacgactcaa ttggcctggg cgaagatggt ccgacgcacc aggctgttga gcaactggcc
      361 agcctgcgct taacgccaaa tttcagcacc tggcgaccgt gcgatcaggt ggaagcggcg
      421 gtgggctgga agctggcggt tgagcgccac aacggaccga cggcactgat cctctcaagg
      481 cagaatctgg cccaggtgga acgtacgccg gatcaggtaa aagagattgc ccgtggcggt
      541 tatgtgctga aagacagcgg cggtaagcca gatattattt tgattgccac cggttcagag
      601 atggaaatcc ccctgcaagc ggcagagaaa ttagcgggag aaggtcgcaa tgtacgcgta
      661 gtttccctgc cctcgactga tattttcgac gcccaggatg aggaatatcg ggagtcggtg
      721 ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag gtattgccga ttactggtac
      781 aagtatgttg gtttgaaagg ggcgattgtt gggatgacag gttatgggga atctgctccg
      841 gcggataagc tgttcccgtt ctttggcttt accgccgaga atattgtggc aaaagcgcat
      901 aaagtgctgg gagtgaaagg tgcctgatgg tgatcgccgg atgctgattg ccggacgctg
      961 attgccggat gcgactctga cgcgtcttat ccggcctaca gcgtcttatc cggcctacat
     1021 gtccccgcca ttttgtttaa cgggtgatcc acaacgtggg ccaggcgtct ggcccatgcc
     1081 agttatcgca ggtgggttct gcggcgtaac gcaccaggcg aaaacgctga ccgtcaaagc
     1141 gccagcgcgc ctgaatgcca caatcgctta atccgcgccc tttcgctaag gtcaccagtt
     1201 cacgcgattt ctcatcaaat gttgcgttca tcagttccag ttcattcgtc tcctgcccgt
     1261 tgttgaacgg caaacgcaac cgaaccgggc gcgaggccag cggattttta cgcgacacaa
     1321 tccatgccaa atcaatggtg ttataggccc ctgcctcaca gctaatcatc atcagcgctt
     1381 tatcatcagt cagcgcagtg acattcacct cacgacgcaa tggatcaagc gagcagcgca
     1441 gaccattcat ccgccagttt ccataatcca gcaaatcgtt gcgttcttcg agtgagagtg
     1501 gcgtcggcgt tgggttaacc accgcgacct ctttcagcgc aggcgcgggc ggtacgctga
     1561 gcggcggttc gtcccctttc ttgatccacg cggtttcact gccaacgcgc ttttgctgag
     1621 catcaataaa caacaacgcc gcttttaagc cactcagcga aatggtctga ttgccgtcgc
     1681 gtaaggtgat cgccttccct tcctgaatca tctgcaaaaa cgcggtgatg gttgccgtat
     1741 catcggttac taataaccat ggtgaaatcc gccacttgtc accacttaac gccaacggct
     1801 cgccatctaa cagcagccgt ggcgctatct ccccttctga cgcctccggc gacttcaatc
     1861 cgccgcgctc aatacgtaaa acggcatcgg tatgcgcccc ggcgctgcgg ctcagggtca
     1921 tcaccagtcc attatgatcg cccgtattac gcgccacgca gaagttttgg ttattacagg
     1981 tgacctgcca gtcggaaaac gcccgttgcg ctggtgccgc ccacaccaaa gacgtcggca
     2041 acaaagcgaa aaaaaagaga aggaaaatgc gatagcgcat ggacggtacg accccagaag
     2101 ttcaaacaaa cagcacaagt cgtatcttcg ttgcgcaggt agcgcggctc aatcggattt
     2161 gtcggattga gccaataata aacaacgaaa tattattgtt atttttcagt ctattaaatg
     2221 tgacgtttgc aaatagttaa gcaataacac cgtcttaccg tcacgtatct cgccggtttt
     2281 gatcatctcc agcgcctggc tgaacggcag ctcgagcact tcaatatctt catcttcgac
     2341 accgccaccg gcgttagcgc gctgattgtc actgtattcg gcgataaaaa agtggattag
     2401 ctcagtcaca ccgcctggcg acatatacag ttcaaataat ttgcgcactt cgcccacttc
     2461 atagcccgtc tcttcaatcg cttctttacg aatgcacact tccggttcgt cgttatccag
     2521 cagcccggcg caggtttcaa tcagctgccc gctttcattg ccattaaccc aggtagcgac
     2581 acggaactga cgaatcagaa ccacggtctt tttcttcgcg ttgtacagga ggatcgtcgc
     2641 accattaccg cgatcgtaaa cttcacgttt atggcggata acttcgccat ctttgcgcgt
     2701 gagatcgtag gtaatgttgt gcagggtgaa atagttatcg gagagaattt tgtctttaat
     2761 gagggtgatt tgttgcgtca taccgactcc acagcgcgaa atgaacaatt atcttacgct
     2821 gtgaagtcgg gtttgtctgc gcaggctatc agtgagattt gactgatttt acccccagcc
     2881 agtcaataat cccctgtgcc gcatgacgtc cttctgccat tgcggtaacc accagatccg
     2941 caccacgcac ggcgtcgcca ccggcgaaga ttttcggatt ggtggtctgg taacggtact
     3001 ggctttccac atccgcgatg atgcggcccc atttgtctac cgttacaccg tgcgactcca
     3061 gccacggcat cccgtgcgga ttgaagccaa acgccataat caccgcgtcg gctggcatga
     3121 caaattcact gccttccacc ggtactggac gccgacgccc ctgggcatcc ggctcgccaa
     3181 gacgcgtgcg caggaaacga atcccgcaaa cgtgaccttg ttcattcagc tcaagcgcca
     3241 ccggctggac gttaaattcg aagttggccc cctcttcgcg ggcgttcttc acctctttct
     3301 tcgagcctgg catgttagct tcatcacgac gataagcgca ggtgacgtta ctcgcgccgt
     3361 ggcgcagcgc agtacgcaca cagtccatcg cggtgtcgcc gccgcccagt accacgacgt
     3421 taagtccggc ggtattgata aacggctctt ccggtagctc ttcgagcccc atcacctgtt
     3481 tagtgttggc gatgaggaac ggcagcgcgt cataaacgcc cggcgcatct tcattgggta
     3541 aacccgcttt catggaacgg taagtgccta cgccaacgaa gaccgcgtcg tattgttcca
     3601 aaagcgaatc caaagagaca tctttaccca cttcacaatt gagttcgaag tgaatcccca
     3661 tcgcgctgaa gatttcccga cggcgtgcca gcagggattt atccagtttg aaagaaggaa
     3721 tgccgaaagt gagcaagcca ccgatttctg gatggcgatc gtacaccgtc accgccacgc
     3781 cattgcgggt cagaacatcc gcacaggcca gccctgccgg acctgccccg ataatcgcca
     3841 cccgcttgtc cactttggtg acatgactta agtcaggacg ccaacctttc gccaacgcct
     3901 gatctgaaat atagcgttca atgttgccga tagttaccgc gccgtgctca tcgcgaatag
     3961 tacaggcacc ttcacacaaa cggtcttgcg gacaaacgcg tccggtaatt tccggcaggg
     4021 tgttggtctg gtgagaaagc tcgacggcgg cgtcgatgtt tccggctttc accagttcaa
     4081 tccactgcgg aatatggtta tgcagcgggc aggtccattc acaaacgctg tgctcgccgc
     4141 acttaaggca gcgcgaggct tcccgttgtg cctggtcggc gcgaaatggc agataaattt
     4201 catcaaaacc ggttttgcgc gcttcaatcg ccagtttatc cggctcgcca cgcgcgggcg
     4261 ttgcctgcat ttgttcgact ttactcatta ccggcatttc ttgtgcagcg gtactggcat
     4321 gccacggttg atgctcctga cgtgcggtgc gcaagcggcg ggatttcgcc atgccggaga
     4381 gtgcgacgtc agtgaccagt tgcagcgcgt ccgccgggca attctcgaca caggcaggac
     4441 cgttttcgcg ccctgcacaa aggtcgcatt tatgcgccgt ggcttttact tttcctgccg
     4501 cgacgggtgt caggacgatt tgcatcgtac caaacggaca ggccaccacg caggatttac
     4561 agccaataca cttttgctga ttgacctgaa tgctgtcatc aacgtggctg attgcgccat
     4621 tagggcagct acgggcgcag ggcgcatctt cacaatggtg acaggtcact gcactacgtt
     4681 gctgctgatg tttgataacc gtaattcggg gatgaaaatg gtgttggctc aggacatgtt
     4741 gctcatcatt gtgagccatg acacaggcga tttcacaagc atgacaaccc agacattgct
     4801 gactgttggc cataataaaa cgattcataa cgaccttctt ttttggttgt aaaaacctta
     4861 ttctttatat gagtgttgtt attacccgac ttacagggga atcggcaatg ttcatgtgcc
     4921 cagaataagt aactatttcg ctataaactg tagcagctca aataatccca tcactgacta
     4981 aattgcgttt cagcgaactg gaacattaat gattttttgt ggagaagacg cgtgtgattg
     5041 ttaaacgacc cgtctcggcc agtctggccc gggccttttt ttacattgtg ctgctgtcga
     5101 ttctttccac gggtatcgct ctgctaactc tggcgagcag tttgcgcgac gctgaggcta
     5161 tcaatattgc cggatcgctg cgtatgcaga gttaccgcct gggctacgac ttgcaaagtg
     5221 gcagtccaca actcaatgca catcgccagt tgtttcagca ggcactgcat tcaccggtat
     5281 taaccaatct caacgtctgg tatgtgccag aagcagtaaa aactcgctat gcgcatctga
     5341 atgccaactg gctggagatg aataatcggc tcagcaaggg cgatttgccg tggtatcagg
     5401 ccaatattaa taattatgtt aatcagatag acctgttcgt gctggcttta cagcactacg
     5461 ctgaacgcaa aatgctgctg gtagtggcga tttccctggc tggcggtatc ggtattttca
     5521 cgctggtctt ttttactttg cgccgtatac gccatcaggt ggttgccccg ctgaatcagc
     5581 tggttaccgc cagtcaacgt attgaacacg ggcagttcga ctcgccgccg ctggatacca
     5641 gtctgccgaa tgagcttggt ctgcttgcaa aaacctttaa ccagatgtcg agcgagctgc
     5701 ataaattgta cctttcgctg gaggcgtcag tagaagaaaa gacccgcgat ctccacgagg
     5761 ccaagcgtcg tctggaggtg ttgtatcaat gttcgcaggc gctgaacaca agtcagattg
     5821 atgtgcattg tttccgccat attttgcaga ttgttcgcga caatgaagcg gctgaatatc
     5881 tggagttaaa tgtcggtgaa aactggcgga ttagcgaagg gcaaccaaac ccggaattgc
     5941 cgatgcagat tttaccggtg acaatgcaag agacggttta cggcgaactg cactggcaaa
     6001 atagtcacgt ttcatcatca gaaccgctgc ttaacagcgt ttcgtcgatg ctgggacgcg
     6061 gtttgtactt taatcaggcg cagaagcatt ttcagcagtt attgttgatg gaagaacgtg
     6121 caaccatcgc ccgcgaattg cacgactcgc tggctcaggt actttcttac ttacgtatcc
     6181 agttgacgtt actgaagcgt tcgataccgg aagataacgc caccgcacaa agtatcatgg
     6241 ccgatttttc ccaggcgttg aatgatgctt atcggcagtt acgcgagctg ttgactactt
     6301 tccgcctgac gctgcagcag gcggatctcc cctccgcgtt gagggaaatg ctggatacgt
     6361 tacaaaatca aaccagcgcc aaactgaccc tcgactgccg tctgccaacc cttgcgctgg
     6421 atgcgcaaat gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg aatgcgatga
     6481 agcacgccaa cgccagcgaa atcgccgtca gttgcgtcac cgcgccggac ggcaatcaca
     6541 cggtttatat ccgtgataac gggattggta tcggtgaacc gaaagaaccc gaaggtcatt
     6601 atggtctgaa tatcatgcgc gaacgcgcgg aacggctggg tgggacgctg actttttcac
     6661 aaccttccgg cggcggcacg ttagtgagta ttagctttcg ctctgcagag ggtgaggaaa
     6721 gtcagttgat gtaatgcctc ctactgacca aagaatactt gcacttaagg ttcagtataa
     6781 aagggcatga taatttacat taactccttt ttttctccac gattggctcg taccttgccg
     6841 ctacagtgaa gcaagtcaag cctacaacga tacgcagaaa cacgaggtcc tcttttaatg
     6901 gcgaatttct ttattgatcg ccccattttt gcctgggtgc tggcaatcct gttgtgtctg
     6961 acagggaccc tggcgatttt ttcattgccc gttgaacaat accccgatct cgcgccaccg
     7021 aatgtgcgag tgaccgctaa ctatcccggc gcatcggccc agacgctgga aaacaccgtg
     7081 ccccaggtta tcgagcaaaa tatgaccggc ctcgataatc tcatgtatat gtcatctcag
     7141 agcagtggca ccggtcaggc atctgtcact ttaagtttta aagcaggcac cgatccggac
     7201 gaagccgtgc agcaagtaca aaaccagctg caatcagcca tgcgaaagtt accgcaggcg
     7261 gtgcaaaatc agggcgtgac ggtgcgtaaa accggcgata ccaatattct gaccattgct
     7321 ttcgtctcta ccgatggttc gatggataaa caggatattg ccgattatgt tgccagtaat
     7381 attcaggacc cgttaagccg cgtgaatggc gtcggggata tcgatgccta tggttcgcaa
     7441 tattccatgc gtatctggct ggccccggcg aaactcaaca gtttccagat gacggctaaa
     7501 gatgtcactg atgccattga gtcacagaac gcgcagattg cggttgggca acttggtggt
     7561 acaccttccg tcgataagca ggcgctcaac gccaccatta acgcccagtc actgctgcaa
     7621 acaccagaac agttccgcga tatcaccttg cgggtgaatc aagacggctc agaggtaagg
     7681 ctgggcgatg tcgccaccgt cgaaatgggg gcggagaaat acgattatct tagccgcttc
     7741 aatggtaagc cagcctccgg gctgggggta aaactggcct ccggcgcaaa cgaaatggcg
     7801 acagggagct ggtgctcaat cgtctcgacg agctggcgca gtacttcccg cacggactgg
     7861 aatacaaggt ggcgtatgaa accacctcgt ttgttaaagc ctccattgaa gacgtggtga
     7921 aaacgctgct ggaagctatc gccctggttt tcctcgttat gtatctgttc ctgcaaaact
     7981 tccgcgccac gctgataccc actatcgccg tgccggtggt gttgatggga accttctccg
     8041 tactttacgc cttcggttac agcgtcaaca ccttaaccat gttcgcgatg gtactggcga
     8101 tcggtctgct ggtggatgac gcaatcgtgg tggtggaaaa cgtcgaacgt attatgagtg
     8161 aggaaggact cactcctcgc gaagccacac gtaaatcgat ggggcagatc cagggggcac
     8221 tggtcgggat tgcgatggtg ctttcggcgg tatttgtacc aatggccttc ttcggcggca
     8281 ccaccggtgc catctatcgc cagttctcta ttaccattgt tgcggcgatg gtgctgtcag
     8341 tactggtagc gatgatcctc actccggctc tgtgtgccac actacttaag ccactgaaaa
     8401 aaggtgagca tcacgggcaa aaaggctttt ttgcctggtt taaccagatg tttaaccgca
     8461 acgccgaacg ctacgaaaaa ggggtggcga aaattctcca ccgcagcctg cgctggattg
     8521 tgatttatgt cctgctgctt ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt
     8581 tcttaccgct ggaagaccgt ggcatgttta ctacctcggt acaattgccc agcggttcaa
     8641 cccaacaaca gaccctgaaa gtcgttgagc aaatcgagaa atattacttc acccatgaaa
     8701 aagacaacat catgtcggtg tttgccaccg ttggttctgg ccctgggggt aacgggcaaa
     8761 acgtggcgcg aatgtttatc cgcctgaaag actggagcga acgcgacagt aagaccggca
     8821 cctcgtttgc cattatcgag cgtgcaacaa aggcgtttaa caagattaaa gaagctcgcg
     8881 ttatcgccag c
//
LOCUS       DAJTMK010000151         8768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTMK010000151 DAJTMK010000000
VERSION     DAJTMK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8768
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.155"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(79..870)
                     /gene="aadA1"
                     /locus_tag="OO135_003235"
     CDS             complement(79..870)
                     /gene="aadA1"
                     /locus_tag="OO135_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5398978.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(983..1813)
                     /locus_tag="OO135_003236"
     CDS             complement(983..1813)
                     /locus_tag="OO135_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5398979.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..3036
                     /gene="intI1"
                     /locus_tag="OO135_003237"
     CDS             2023..3036
                     /gene="intI1"
                     /locus_tag="OO135_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5398980.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3239..3589
                     /locus_tag="OO135_003238"
     CDS             3239..3589
                     /locus_tag="OO135_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5398981.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3715..4275
                     /locus_tag="OO135_003239"
     CDS             3715..4275
                     /locus_tag="OO135_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5398982.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4278..7244
                     /locus_tag="OO135_003240"
     CDS             4278..7244
                     /locus_tag="OO135_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5398983.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7311..7688
                     /locus_tag="OO135_003241"
     CDS             7311..7688
                     /locus_tag="OO135_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5398984.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7889..8548)
                     /locus_tag="OO135_003242"
     CDS             complement(7889..8548)
                     /locus_tag="OO135_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5398985.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2478 c         2348 g         1992 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa
     2821 caagcaggca tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc
     2881 ttgctccgca gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc
     2941 tctacgacga tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg
     3001 cttgatgcgc tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg
     3061 attcactacc cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc
     3121 ctccctgcgt ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc
     3181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat
     3241 ggaggtggta gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg
     3301 ttgcgacacg gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg
     3361 cttcgtcaac caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg
     3421 cgtgtgctgc aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt
     3481 ggagcatttc tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac
     3541 cgaaaccagc gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta
     3601 acctgatgtc agatgccatg tgtaaattgc gtcaggatag gattgaattt tgaatttatt
     3661 gacatatctc gttgaaggtc atagagtctt ccctgacatt ttgcagggaa ttccatgact
     3721 ggacagcgca ttgggtatat cagggtcagc accttcgacc agaacccgga acggcaactg
     3781 gaaggcgtca aggttgatcg cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt
     3841 ccgcaactgg aagcgctgat aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc
     3901 atggatcgcc tggcgcgcaa tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa
     3961 cgcggcgtgc atatcgaatt cgtcaaggaa cacctcagtt ttactggcga agactctccg
     4021 atggcgaacc tgatgctctc ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc
     4081 cgcgagcgtc agcgcgaggg tattgcgctc gccaagcaac gcggggctta ccgtggcagg
     4141 aagaaatccc tgtcgtctga gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag
     4201 caaaagacca agcttgctcg tgaattcgga atcagtcgcg aaaccctgta tcaatacttg
     4261 agaacggatc agtaaatatg ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc
     4321 tgctggcgtt gccggactcc aaggacgacc tgatccgaca ttacacattc aacgataccg
     4381 acctctcgat catccgacag cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc
     4441 tctgttacct gcgctttccc ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct
     4501 tgttgaagct ggtcgccgac cagctcaagg tcggcgtcga aagctggaac gagtacggcc
     4561 agcgggagca gacccggcgc gagcacctga gcgagctgca aaccgtgttc ggtttccggc
     4621 ccttcaccat gagccattac cggcaggccg tccagatgct gaccgagctg gcgatgcaaa
     4681 ccgacaaagg catcgtgctg gccagcgcct tgatcgggca cctgcggcgg cagtcggtca
     4741 ttctgcccgc cctcaacgcc gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc
     4801 ggcgcatcta cgacgccttg gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg
     4861 atctgctcaa gcgccgggac aacggcaaga cgacctggtt ggcttggttg cgccagtctc
     4921 cggccaagcc aaattcgcgg catatgctgg aacacatcga acgcctcaag gcatggcagg
     4981 cactcgatct gcctaccggc atcgagcggc tggttcacca gaaccgcctg ctcaagattg
     5041 cccgcgaggg cggccagatg acacccgccg acctggccaa attcgagccg caacggcgct
     5101 acgccactct cgtggcgctg gccaccgagg gcatggccac cgtcaccgac gaaatcatcg
     5161 acctgcacga ccgcatcctg ggtaagctgt ttaacgctgc caagaataag catcagcagc
     5221 agttccaggc gtcaggcaag gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc
     5281 aggcgctgat cgacgccaag caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca
     5341 tgtcctggga ttcctttgcc gagagcgtca ccgaggcgca gaagctcgcg caacccgatg
     5401 acttcgattt cctgcatcgc atcggcgaga gctacgccac cctgcgccgc tatgcaccgg
     5461 aattccttgc cgtgctcaag ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca
     5521 ttgaggtgct gcgcggcatg aacaccgaca acgcccgcaa gctgccagcc gatgcaccga
     5581 ccggcttcat caagccgcgc tggcagaaac tggtgatgac cgacgccggc atcgaccggc
     5641 gctactacga actgtgcgcg ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct
     5701 gggtgcaggg ttcacgccag ttcaaggact tcgaggacta cctggtaccg cccgagaagt
     5761 tcaccagcct caagcagtcc agcgaattgc cgctggccgt ggccaccgac tgcgaacaat
     5821 atctgcatga gcggctgacg ctgctggaag cacaacttgc caccgtcaac cgcatggcgg
     5881 cagccaacga cctgccggat gccatcatca ccgagtcggg cttgaagatc acgccgctgg
     5941 atgcggcggt gcccgacacc gcgcaggcgc tgatagacca gacagccatg gtcctgccgc
     6001 acgtcaagat caccgaactg ctgctcgaag tcgatgagtg gacgggcttc acccggcact
     6061 tcacgcactt gaaatcgggc gatctggcca aggacaagaa cctgttgttg accacgatcc
     6121 tggccgacgc gatcaacctg ggcctgacca agatggccga gtcctgcccc ggcacgacct
     6181 acgcgaagct cgcttggctg caagcctggc atacccgcga cgaaacgtac tcgacagcgt
     6241 tggctgaact ggtcaacgct cagtttcggc atccctttgc cgggcactgg ggcgatggca
     6301 ccacatcatc atcggacgga cagaatttcc gaaccgctag caaggcaaag agcacggggc
     6361 acatcaaccc aaaatatggc agcagcccag gacggacttt ctacacccac atctccgacc
     6421 aatacgcgcc attccacacc aaggtggtca atgtcggcct gcgcgactca acctacgtgc
     6481 tcgacggcct gctgtaccac gaatccgacc tgcggatcga ggagcactac accgacacgg
     6541 cgggcttcac cgatcacgtc ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc
     6601 gcatccgcga cctgggcgac accaagctct acatcccgaa gggcgatgcc gcctatgacg
     6661 cgctcaagcc gatgatcggc ggcacgctca acatcaagca cgtccgcgcc cattgggacg
     6721 aaatcctgcg gctggccacc tcgatcaagc agggcacggt gacggcctcg ctgatgctca
     6781 ggaaactcgg cagctacccg cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc
     6841 gcatcgagcg cacgctgttc atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg
     6901 tgcatgccgg gctgaacaag ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca
     6961 accgccttgg tgaaatccgt gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc
     7021 tcaacctggt gacggcggcc atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc
     7081 atgcgttgcg cggcaatggt catgccgtcg atgactcgct attgcagtac ctgtcgccac
     7141 tcggctggga gcacatcaac ctgaccggtg attacctatg gcgcagcagc gccaagatcg
     7201 gcgcggggaa gttcaggccg ctacggcctc tgcaaccggc ttagcgtgct ttattttccg
     7261 ttttctgaga cgacccctat tatggcgaag gtatcattca ggaagcgttg atgaaggatt
     7321 atctctgtaa aaagttattc aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg
     7381 gcaataacat tactggcctg gcatgttgca atattcttta tccctcgccc cgatacagcg
     7441 gtcagctgca tattaaagag ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta
     7501 aagcgataat gcgctttata gcccggcttg cgcttgaaca ggaatgcctt agccttagct
     7561 ggaacgctga aaaatccaac cccggcgcta accgttttta tcaggctctg ggaggcagaa
     7621 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7681 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7741 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7801 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7861 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     7921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     8041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     8101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     8161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     8221 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     8281 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     8341 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     8401 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     8461 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8521 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8581 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8641 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     8701 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     8761 aaagtgcg
//
LOCUS       DAJTMK010000152         8734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTMK010000152 DAJTMK010000000
VERSION     DAJTMK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.156"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            244..1155
                     /gene="ttdA"
                     /locus_tag="OO135_003243"
     CDS             244..1155
                     /gene="ttdA"
                     /locus_tag="OO135_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR5398986.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETATVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1152..1757
                     /gene="ttdB"
                     /locus_tag="OO135_003244"
     CDS             1152..1757
                     /gene="ttdB"
                     /locus_tag="OO135_003244"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5398987.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1806..3269
                     /gene="ttdT"
                     /locus_tag="OO135_003245"
     CDS             1806..3269
                     /gene="ttdT"
                     /locus_tag="OO135_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5398988.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO135_003246"
     CDS             complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO135_003246"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5398989.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4562..4777
                     /gene="rpsU"
                     /locus_tag="OO135_003247"
     CDS             4562..4777
                     /gene="rpsU"
                     /locus_tag="OO135_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5398990.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4888..6633
                     /gene="dnaG"
                     /locus_tag="OO135_003248"
     CDS             4888..6633
                     /gene="dnaG"
                     /locus_tag="OO135_003248"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5398991.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMTYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6828..8669
                     /gene="rpoD"
                     /locus_tag="OO135_003249"
     CDS             6828..8669
                     /gene="rpoD"
                     /locus_tag="OO135_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5398992.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2108 a         2331 c         2333 g         1962 t
ORIGIN      
        1 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
       61 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
      121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
      181 acatttttag tggtagtttg tggcggtgaa tttttccaga caaatacaaa aactggagtt
      241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
      301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
      361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
      421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
      481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
      541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
      601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
      661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gtggcggctg cacgctacct
      721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
      781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
      841 gccacctcgg tggaaaccgc caccgtactc tcgcgtaaag ccattttgcg cccgattggc
      901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
      961 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
     1021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg
     1081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     1141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     1201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     1261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     1321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     1381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     1441 aactggtggt tggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
     1501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag
     1561 agattgaaga agtgcactgg acagagctcg gaatgccgga gtcgctgtgg gtctgccggg
     1621 tcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaat ctgatagccg
     1681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     1741 tccattacat caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata
     1801 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
     1861 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
     1921 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
     1981 tggtgggtat ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc
     2041 tcgcccagcc aggctttaag ttcaccgcca aatccctctc ctgggcagtt tctggttttt
     2101 ctaattcggt tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
     2161 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
     2221 ttctcggcta tgcggtgatg ttctccgagc tgatcctcgc acctgtaaca ccgtccaact
     2281 cggcgcgtgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
     2341 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
     2401 ttgccgactg cgtgaccagc gccattttct tgacggcgat ggcaccaaac ttgctgttaa
     2461 ttggactgat gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga
     2521 tgttgccgct cagtattttg ctggtgctgc tggttccctg gctggcttat gtgctgtacc
     2581 cgccggtact gaagtctggc gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa
     2641 tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc
     2701 tgtggatttt cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcac
     2761 tgatgctgct tctgcgcatt atcagttggg acgacattgt cagtaataaa gcggcgtgga
     2821 acgttttctt ctggctggcc tcgcttatca ccctcgctac cggactcaac aacactggtt
     2881 ttattagctg gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatgg
     2941 tgatggtggc gttgattgtg gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg
     3001 cgtatacctc cgccctcgca ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc
     3061 cgctgccggt gttctgcctg atggttggcg cggcaattgg tctggggagc attcttacac
     3121 catacgccac cggacccagt ccgatttact acggtagtgg ttatctgcca acggcggatt
     3181 actggcgact gggggcgatt tttgggctga tattcctcgt gttgctggtg attaccggct
     3241 tactgtggat gcccgtggtg ttgctttaag tgaggacggt gccggaaatt gctctccggc
     3301 acccaatagt tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg
     3361 ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg
     3421 ccgttatcag tacaaaactc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc
     3481 atttccgcca gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt
     3541 cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa tcatcaacgt atcgaccacc
     3601 gcatcttcaa aggcccgggc gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga
     3661 atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga aatccagccc cggacggtcg
     3721 gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct gagccgccat tttcgacagt
     3781 aacggtccgc caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg
     3841 gcggcatcat cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt
     3901 aactgcgtat gaccgccgga caccagcagc gcgacaaacg gaaattcagg cgggttatct
     3961 tccagcatcg gcgctaacag atgcccttcc atatggtgta ccgggattgc cggaacgttc
     4021 caggcaaacg ccagagaacg ccccacggtt gcgccaacca gcagtgcgcc gactaatcca
     4081 gggcctgcgg tataggccac agcatcaatg tcttttgccg ttaagccaga ctcctttagc
     4141 gccgcctgga tcaacggtac ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg
     4201 acgccgccgt agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct
     4261 ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca
     4321 cgcatgactt gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg
     4381 taaaactttg ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaaatcc
     4441 ctttcaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
     4501 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
     4561 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
     4621 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
     4681 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
     4741 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
     4801 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
     4861 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
     4921 gatctgctgg cacgcactga cattgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
     4981 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
     5041 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
     5101 gacttcctga tgacctacga caagcttgag ttcgtcgaaa cggtcgaaga gctggcagca
     5161 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
     5221 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
     5281 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
     5341 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
     5401 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
     5461 gatcagggac gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa
     5521 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
     5581 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
     5641 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
     5701 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
     5761 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
     5821 cgtgcaggcc gtgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
     5881 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
     5941 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
     6001 ctgtttaaca gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
     6061 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
     6121 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
     6181 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
     6241 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
     6301 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
     6361 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
     6421 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
     6481 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
     6541 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
     6601 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
     6661 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
     6721 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
     6781 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
     6841 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
     6901 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
     6961 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
     7021 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
     7081 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
     7141 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
     7201 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
     7261 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
     7321 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
     7381 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
     7441 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
     7501 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
     7561 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
     7621 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
     7681 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
     7741 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
     7801 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
     7861 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
     7921 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
     7981 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
     8041 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
     8101 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
     8161 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
     8221 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
     8281 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
     8341 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
     8401 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
     8461 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
     8521 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
     8581 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
     8641 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
     8701 ccggcactag tccctctgca caaacgccac cttt
//
LOCUS       DAJTMK010000153         8662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTMK010000153 DAJTMK010000000
VERSION     DAJTMK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.157"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            63..839
                     /gene="eutC"
                     /locus_tag="OO135_003250"
     CDS             63..839
                     /gene="eutC"
                     /locus_tag="OO135_003250"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCR5398993.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            849..1508
                     /gene="eutL"
                     /locus_tag="OO135_003251"
     CDS             849..1508
                     /gene="eutL"
                     /locus_tag="OO135_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5398994.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1521..2021
                     /gene="eutK"
                     /locus_tag="OO135_003252"
     CDS             1521..2021
                     /gene="eutK"
                     /locus_tag="OO135_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5398995.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKESGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2067..3119
                     /gene="eutR"
                     /locus_tag="OO135_003253"
     CDS             2067..3119
                     /gene="eutR"
                     /locus_tag="OO135_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5398996.1"
                     /translation="MKKTRTANLHHLYHEPFPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLGLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3125..4024)
                     /gene="hemF"
                     /locus_tag="OO135_003254"
     CDS             complement(3125..4024)
                     /gene="hemF"
                     /locus_tag="OO135_003254"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5398997.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4028..4897)
                     /gene="amiA"
                     /locus_tag="OO135_003255"
     CDS             complement(4028..4897)
                     /gene="amiA"
                     /locus_tag="OO135_003255"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5398998.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5111..5536
                     /locus_tag="OO135_003256"
     CDS             5111..5536
                     /locus_tag="OO135_003256"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5398999.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5523..5972
                     /locus_tag="OO135_003257"
     CDS             5523..5972
                     /locus_tag="OO135_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5399000.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6179..6754
                     /locus_tag="OO135_003258"
     CDS             6179..6754
                     /locus_tag="OO135_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5399001.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6850..7749
                     /gene="yfeX"
                     /locus_tag="OO135_003259"
     CDS             6850..7749
                     /gene="yfeX"
                     /locus_tag="OO135_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5399002.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSPDKLMAL"
     gene            complement(7807..8662)
                     /gene="pbp4b"
                     /locus_tag="OO135_003260"
                     /pseudo
     CDS             complement(7807..8662)
                     /gene="pbp4b"
                     /locus_tag="OO135_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
BASE COUNT         1981 a         2193 c         2440 g         2048 t
ORIGIN      
        1 ccggacaaac ggcccccgcg ctgtcagaag caaagtgcac caccaccacc tgtgcggcac
       61 cggtgacctc ggaaagctgc gcgctggatt taggttctgc ggaagcaaaa gcgtggattg
      121 gtgttgaaaa cccgcatcgc gcagacgtat taacagaact gcgtcgcagc accgtggccc
      181 gcgtttgtac cggtcgtgcc ggtccgcgtc cgcgtaccca ggcgttgctg cgtttcctgg
      241 ccgatcactc tcgttcgaaa gataccgtac tgaaagaagt gccggaagag tgggtgaaag
      301 cgcagggctt gctggaagta cgctccgaaa ttagcgacaa aaatctctac ctgactcgcc
      361 cggatatggg ccgccgcctg tgtgcagaag ctgttgaagc gctgaaagca cagtgtgttg
      421 ctaatccgga cgtacaggtc gttatttctg atggcctgtc aactgatgcc atcaccgtga
      481 actacgaaga gatcctgccg ccgctgatgg cgggcctgaa acaggccggg ctgaaagtcg
      541 gcacgccatt ctttgttcgt tatggtcgcg tgaagattga agatcagatc ggtgagatcc
      601 tcggcgcgaa agtggtgatc ctgctggtgg gggaacgtcc ggggttgggt cagtcagaaa
      661 gcctctcctg ttacgccgtt tactcgccgc gtatggcgac caccgttgag gccgatcgca
      721 cctgcatctc taacattcac cagggcggca cgccgccggt tgaagccgca gccgtcattg
      781 tggatttggc gaaacgtatg ctggagcaga aagcatccgg catcaacatg acccgataag
      841 gaggcatcat gccagcttta gatttgattc gaccgtcggt aaccgccatg cgggtgattg
      901 cctccgttaa cgctgaattt gcgcgtgaac taaaattgcc gccgcatatt cgtagcctcg
      961 ggctgatttc tgctgattcc gatgacgtca cctacattgc ggctgacgaa gcgaccaagc
     1021 aggcgatggt cgaagtggta tatggccgct cgctgtatgc gggagcagca cacggaccgt
     1081 caccgactgc cggtgaagtg ctgattatgc ttggtgggcc aaacccggcg gaagtgcgtg
     1141 ctggtctgga tgcgatggtt gcgcatattg aaaatggcgc tgctttccag tgggctaatg
     1201 acgcagaaaa caccgcattc ctggcacatg tagtttcgcg taccggttct tatctctcat
     1261 caaccgccgg gatcacgctt ggcgatccga tggcgtatct ggtggcaccg ccgctggaag
     1321 cgacctacgg cattgatgca gcgttgaaat ctgccgacgt acagctggtg acctatgtcc
     1381 cgccaccgtc tgaaaccaac tactcggcag catttttaac cgggagccag gccgcgtgta
     1441 aagcagcctg taacgccttt accgatgcag tgctggaaat cgcgcgtaat ccaatccagc
     1501 gtgcgtaacg gaggttgccg atgatcaatg cactgggatt gctggaagtg gacggcatgg
     1561 tcgccgcgat agatgcagcg gatgccatgc tcaaagcagc taacgttcgt ctgctcagtc
     1621 acgaagtgct tgaccctggt cgcttaacgc tggtggtgga aggcgatctg gcggcgtgtc
     1681 gtgcggcgct ggacgcaggt tgtgctgccg cgatgcgtac cgggcgtgtc atcagccgca
     1741 aggagagcgg tcggccagac gatgacaccc agtggctggt tactggcttt aaccgccagc
     1801 cgaagcaacc cgtaagggaa cccgacgcgc cagttatcgt cgcggaatct gctgacgagt
     1861 tgttggcgct gttaacatca gtacgtcagg gaatgacggc aggagaagtg gctgcccact
     1921 ttggctggcc gctggaaaaa gccagaaatg cgctcgaaca gctcttttct gccgggacgt
     1981 tacgtaaacg cagtagtcgt tatcgtctca agccccatta acctgtcgga ggtgccgggt
     2041 gtcctacaac acccggcatt aacatcatga aaaagacccg tacagccaat ttgcaccatc
     2101 tttatcatga acccttcccc gaaaacctga agctcacgcc gaaggtcgaa gtggataatg
     2161 ttcatcaacg acagacaacg gatgtctatg aacatgcttt aacgattacc gcctggcagc
     2221 agatttacga tcagctgcat ccgggcaagt ttcatggtga gtttacggaa attctactcg
     2281 atgatattca ggtttttcgt gaatacaccg gtctggcgct gcgtcagtcg tgcctggtct
     2341 ggccgaactc gttctggttt ggcattccgg cgacgcgcgg tgagcaggga tttatcggtt
     2401 cgcaatgtct gggaagcgcg gaaatcgcca cccgccctgg tggcactgaa tttgaactga
     2461 gcacgccgga tgattacacg atcctgggcg tggtgctttc tgaagatgtc atcacccggc
     2521 aggctaactt tttgcataac ccggatcggg tattacatat gctgcgtagc cagtcggcgc
     2581 tggaagtgaa agagcagcat aaagccgcgc tgtggggctt tgtccaacag gcgctggcga
     2641 cgttttgcga gaacccggaa aatctccatc agcctgcagt gcgaaaagtg ctgggggata
     2701 atttgctaat ggcgatgggg gcgatgctgg aagacgcgca accaatggtg acggcggaaa
     2761 gcatcagtca tcagagttac cgtagattac tttcccgcgc ccgtgaatat gtgctggaaa
     2821 atatgtctga gccggtgacg gtgctgggct tgtgtaatca actgcatgtc agtcgccgca
     2881 cgctacaaaa cgcgtttcac gctattttag gcattggccc aaacgcgtgg ctgaaacgca
     2941 ttcgcctgaa cgccgtacgc cgcgaactga taagcccgtg gtcgcaaagc acaacggtaa
     3001 aagacgccgc catgcagtgg ggattctggc atctggggca atttgtcacg gattaccagc
     3061 agctgtttgc cgagaagccg tcgttgacgt tgcatcagcg gatgcgggaa tgggggtgag
     3121 ggagttacac ccaatccctg accttaataa actcacttaa cgccgcttct gggctgccat
     3181 cttttggctg ataatcatac tcccagcgta ccagtggcgg cattgacatc aggatcgact
     3241 cggtgcgccc gccagtttgc aggccaaaca gagtgccgcg atcccagacc agattgaact
     3301 cgacataacg accgcgacgg tagagctgaa aattgcgctc gcgctcgccg taggccatcg
     3361 ctttacgtcg ctctacaatt ggtaaataag cgtcggtgta gcctttgcct accgcctgca
     3421 taaaggcaaa acagtggtcg aaatctggcg tgttcagatc atcaaagaac agcccgccaa
     3481 taccgcgctg ttcgttgcga tgtttgaggt agaagtaatc gtcgcaccac tttttgtaac
     3541 ggggataaac gtcttcacca aatggcaggc acaggtcacg ggcggtgcgg tgccagtgaa
     3601 tggcgtcttc ttcaaaacca tagaaagggg ttaaatcgaa gccgccgcca aaccaccaga
     3661 cgggctctgc acccggtttt tcggcaataa aaaaacgcac attcgcgtgg ctggtgggaa
     3721 catacgggtt atgcggatgc actaccagtg aaacgcccat cgcctcgaaa ctgcgcccgg
     3781 caagttccgg gcgatgagcg gtggcggaag caggcatcgc ctcgccgtgg acgtgagaaa
     3841 agttgacgcc tgcctgttcg aaaacaccac cattacgcaa cacccgacta cgcccgccgc
     3901 cgccagcttc gcgctgccaa ctatcttcga caaattctgc gccatcgacg gcggtcagct
     3961 gctgacaaat cgtatcctga aggttgagca gaaactgttt aacctggtgt gcgtcgggtt
     4021 tcataactta tcgctttttc gaatgtgctt tctggttgtc gaaccagtgg aaataactga
     4081 tcacgccttc agcaatcgct gtggcgattt tctgacgaaa cgccgccgtg cctaacagcc
     4141 gctcttcttc cgggttggtg ataaacgagg tttccaccag caccgaagga accgacggcg
     4201 atttcaacac cacaaacgcg gcttgttcgg tgttgcggct gtgcagttta tgcaccggct
     4261 taatcttttt cagaatatgc gagccaagcg tcaggctgtt tttaatagta tcggtttgca
     4321 ccagatcaaa cagcacttgc tgcaataggt gatccttgtc agtcgccttt ttaccggcaa
     4381 cttcatcggc gcggttttcg cgttcagaca ggtatttcgc cattgcgcta cttgccccac
     4441 ggttagagag ggcaaatacc gaagcaccgg cagctttcgg gttggtaaag ccatcggcat
     4501 gaattgacat aaacagatct gcgccatgtt tatgggcgat ttcaacgcga tcgtaaagtg
     4561 ggataaacgt atcgccagaa cgcgttaaac gcgcatcaat cccatgatta cgcaaaatgg
     4621 aacggacgtt tttagcaatc gccagcacca catgtttttc tttcgaaccg ttgcgtccga
     4681 tcgctccggt atcaattccg ccgtggcctg gatcgagaac aacgacacgt ttgccgccag
     4741 attttttggc tttcggcttg ctgtgtccgt tgctggtttt taaaggttcg tctttggcga
     4801 tggcttgcga cattcctgac aacgtcaggg cagccaaacc ggctttcagc acctggcggc
     4861 gcgaagtgag tgtttttagt ggtttaaaag tgctcatacg gcctgagttg taataataaa
     4921 gttccagatg ttatatcgta tcgcgtaatc cgttacgacg gtttgatgtt gagaattgtt
     4981 ttacttttca tttcaatagt tgacaactcc ccattatgcc actttttcaa ccgattttcg
     5041 tcagatattg gctaattcta ccgttcacgc cataatcagc cttttaaccc gcaaaaaggt
     5101 aacgaatacc atggagatac gcgtatttcg ccaggaagat ttcgaagagg tcatcaccct
     5161 ttgggagcgt tgcgacttgc tgcgtccgtg gaacgatccg gaaatggaca tcgagcgtaa
     5221 gatgaaccat gacgtcagtt tgtttttggt cgctgaggtg aacggtgaag tggtcggaac
     5281 ggtgatgggc ggttatgacg ggcatcgcgg gtctgcttat tatcttggcg tgcatccaga
     5341 gtttcgtggg cgtgggattg ccaatgcgtt gcttaatcgg ctggagaaaa agctgattgc
     5401 tcgtggctgc ccgaaaattc agatcaatgt gccggaagat aacgacatgg tgctcggaat
     5461 gtatgaacgc ctgggatatg aacacgccga catgctgagt ctgggtaagc gtttgattga
     5521 agatgaagag tactgagttc catcctgtcc attatgatgc gcacggacgc ctgcgtttac
     5581 ccttgctctt ctggcttgtg ctattgcttc aggcgcgaac ctgggtgctg tttatcattg
     5641 ccggtgcgtc gcgcgagcag ggtactgcgc tgctaaatct gttttatccc gatcatgata
     5701 atttctggct ggggttgatt cccggtattc cggcggtgct ggcgtttttg ttgagcggtc
     5761 ggcgggcttc gtttcctcgc acctggcatg tgctctattt tctgttgctg ttggcgcagg
     5821 tggttttact ttgctggcaa ccgtggctgt ggctgaacgg tgaatccgtt agcggtatcg
     5881 gtctggcgct ggtagtggcg gatattgtgg cgttaatctg gctgctgacc aatcgacgtt
     5941 tacgcgcttg ttttaatgaa gaaaaagaat aacgcagagg tgcgctttgt ttatgccgga
     6001 tgcggcgtga acgccttatc cggcctacaa aacatcgaaa attcaatata ttgcaggaga
     6061 aagtaggcct gataagcgta gcgcatcagg caattttgtg tttgtcatca acctttaacg
     6121 gcactttttg gtgaatttgc actccaagca acgttattga ataaccaaag gaagtgacat
     6181 gaaatcgctg cgtttaatgt tatgcgctat gccgttgatg ctgaccggct gttccacgat
     6241 gtcgtcagtt aactggtcgg ccgctaaccc gtggaactgg tttggatcgt ccaccaaagt
     6301 gagcgagcag ggcgtgggtg aattaacggc gtccacacca ctgcaagaac aagccattgc
     6361 tgatgcgctt gatggcgatt atcgcctgcg cagcggaatg aaaaccgcga acggcaacgt
     6421 ggtgcgcttt tttgaagtaa tgaaaggcga caacgtggcg atggtgatta acggcgatca
     6481 aggcacgatc agtcgcattg acgtgctgga tagcgatatt cctgctgaca ccggtgttaa
     6541 aatcggtaca ccgtttagcg acctttacag caaagcattt ggcaattgcc aaaaagctga
     6601 tggtgatgat aatcgtgccg tcgaatgtaa agccgaaggc agtcaacata ttagctacca
     6661 gttcagtggg gaatggcgtg gtcctgaagg gttaatgcct tcggacgata ccctgaaaaa
     6721 ctggaaagtc agtaaaatta tctggcggcg ttaatttgcg tctgaacaaa ccgccgccgc
     6781 gaaaaaacac gtaaaatagc gctcaacaat gccacggatt gcgtggcatt cttattttca
     6841 ggaggaacaa tgtctcaggt tcagagtggc attttgccag aacattgccg cgcggcgatt
     6901 tggatcgaag ccaacgtgaa aggggaagtt gacgccctgc gtgcggccag taaaacattt
     6961 gccgacaaac tggcaacttt tgaagcgaaa ttcccggacg cgcatcttgg tgcggtggtt
     7021 gcctttggta acaacacctg gcgcgctctg agcggcggcg ttggggcaga agagctgaaa
     7081 gattttccgg gctacggtaa aggccttgcg ccgacgaccc agttcgatgt gttgatccac
     7141 attctttctc tgcgtcacga cgtaaacttc tctgtcgctc aggcggcgat ggaagccttt
     7201 ggtgactgca ttgaagtgaa agaagagatc cacggcttcc gttgggtgga agagcgtgac
     7261 ctgagcggct ttgttgacgg tacagaaaac ccggcgggtg aagagacgcg ccgcgaagtg
     7321 gcggttatca aagacggcgt ggatgccggc ggcagctatg tgtttgtgca gcgttgggag
     7381 cacaacctga agcagctcaa ccggatgagc gttcacgatc aggagatgat gatcgggcgc
     7441 accaaagagg ccaacgaaga gattgacggc gacgaacgtc cggaaacctc tcacctcacc
     7501 cgcgttgatc tgaaagaaga tggcaaaggg ctgaagattg ttcgccagag cctgccgtac
     7561 ggcaccgcca gtggcactca cggtctgtac ttctgcgcct actgcgcgcg tctgcataac
     7621 attgagcagc aactgctgag catgtttggc gataccgatg gtaagcgtga tgcgatgttg
     7681 cgtttcacca aaccggtaac cggcggctat tacttcgcgc cgtcgccgga caagctgatg
     7741 gcgctgtaag cgtttctgat tctatgatta aaggccagcc agtaacaaaa ctgactggcc
     7801 tttttattac ttttgcttca acgccgcata cacctgatca actacccaac cataagttgc
     7861 aatcggtaac tgaccgcttt cgaacatatt gggatttttt tgtggatcgg caactggcga
     7921 atggggcttg ttgcttaaca tcacaatcgc catatgattg accggatcga taacggttac
     7981 tgtcccggtc cagccagtgt gaccgtaggt ttgcgggctt gccagcgtgc caaacgtcgg
     8041 cgtcatggtg gcattaccat tcacgcgcca gccgaggcca aaagtggcat cttccttaga
     8101 gctggtggtg aacatcttca ccgtttccgc actgaacagc tgcacatcgc catagccccc
     8161 gccgttcagc atcgtttgca ttaacaccgc aatatcgccg gtattggaaa acaaacctgc
     8221 gtgcccggaa acgccgccca tcgaataaaa ggctttttca tcgtgcacct gaccccagag
     8281 agtggaggtg cggatattcg ggaaatggat cacgccatcg cgggtattgc cgttcaattc
     8341 cgtggcggca atttgttgcg gtttaaagcc tttcagcagc gggttaaata ccgtatgtgt
     8401 caggccgagc gggcgataaa tcgactcttc aacatagcgg tcaagcggct gaccggtaac
     8461 cgactcgacg ataaatccaa gcagcatata atcgacatcg ctgtagatat gtttgctgcc
     8521 gggctgatat tccagcggcg tgcgcttgat catctccagc gtctggcctt tatcctagga
     8581 atataacgcg cccgcgaccg ctttattcgg gtattgcgga tctgccggga aaccgccgct
     8641 gtgatgcagc aggtcagaaa tc
//
LOCUS       DAJTMK010000154         8630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTMK010000154 DAJTMK010000000
VERSION     DAJTMK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8630
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.158"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            614..2542
                     /gene="thrS"
                     /locus_tag="OO135_003261"
     CDS             614..2542
                     /gene="thrS"
                     /locus_tag="OO135_003261"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5399003.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2546..3088
                     /gene="infC"
                     /locus_tag="OO135_003262"
     CDS             2546..3088
                     /gene="infC"
                     /locus_tag="OO135_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5399004.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3185..3382
                     /gene="rpmI"
                     /locus_tag="OO135_003263"
     CDS             3185..3382
                     /gene="rpmI"
                     /locus_tag="OO135_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5399005.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3435..3791
                     /gene="rplT"
                     /locus_tag="OO135_003264"
     CDS             3435..3791
                     /gene="rplT"
                     /locus_tag="OO135_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5399006.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3908..4032
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3914..3958
                     /gene="pheM"
                     /locus_tag="OO135_003265"
     CDS             3914..3958
                     /gene="pheM"
                     /locus_tag="OO135_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5399007.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4241..5224
                     /gene="pheS"
                     /locus_tag="OO135_003266"
     CDS             4241..5224
                     /gene="pheS"
                     /locus_tag="OO135_003266"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5399008.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDSFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5239..7626
                     /gene="pheT"
                     /locus_tag="OO135_003267"
     CDS             5239..7626
                     /gene="pheT"
                     /locus_tag="OO135_003267"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5399009.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKALLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWSGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7631..7930
                     /gene="ihfA"
                     /locus_tag="OO135_003268"
     CDS             7631..7930
                     /gene="ihfA"
                     /locus_tag="OO135_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5399010.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(8234..8374)
                     /locus_tag="OO135_003269"
     CDS             complement(8234..8374)
                     /locus_tag="OO135_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5399011.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2209 a         2039 c         2276 g         2106 t
ORIGIN      
        1 agctctgatg aaggcgttgg tatgtcgcta tcgatgtgca atctatgagg agacttataa
       61 tgagttctga taatgatatc gttttgactc ataatctatt caccttatat atactggagg
      121 ttttgaggat gatgattaat tattatcgcc agaacgaaat gtgcacgaaa aattgctata
      181 agaattgtgt cacttttatc attgtgatat gaccacttcg tcattaatct catgatcgtt
      241 taaatactac cgccaaatca cgcaacgaac tttgctggat tcagtttatg ctgctgtaaa
      301 tccgctcgca gtaaaccttt cagacgcacc gtgatgttat cagtggttct ttaagcgttt
      361 tgctggtgta ctcactacaa acgaattgcg aatcgaatca atgtgaaacg gaaaggtaca
      421 atctcccttt ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag gttaatgtct
      481 ttcatttgtg tacctgattg cgaaccaatt tagcatttgt tggctagatg gtttcgcaat
      541 gaactgttaa taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc accactgcaa
      601 ataaggatat aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca
      661 cgctgtaagc cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat
      721 cgcagggcgc gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca
      781 actgtcgatc attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc
      841 gcacctgtta gggcatgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc
      901 ggttattgac aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga
      961 tgtcgaagca ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa
     1021 gaagaaagtc agctggcacg cagcgcgtga aactttcgcc aaccgtgggg agagctacaa
     1081 agtctccatt cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga
     1141 agaatatgtc gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt
     1201 caaactaatg aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca
     1261 acgtatttac ggtacggcgt gggcagacaa aaaagcactt aacgcttacc tgcagcgcct
     1321 ggaagaagcc gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat
     1381 gcaggaagaa gcgccgggta tggtattctg gcacaacgac ggctggacca tcttccgtga
     1441 actggaagtg tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc
     1501 gttcatgatg gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc
     1561 aatgttcacc acatcttctg agaaccgtga atactgcatt aagccgatga actgcccggg
     1621 tcacgtacaa attttcaacc aggggctgaa gtcttatcgc gatctgccgc tgcgtatggc
     1681 cgagtttggt agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgcgt
     1741 gcgtggattt acccaagatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga
     1801 agttaacgga tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat
     1861 cgtcgtcaaa ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg
     1921 tgctgaggcg gacctggcgg ttgcgctgga agaaaacaac atcccgtttg aatatcaact
     1981 gggtgaaggc gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg
     2041 tgcatggcag tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc
     2101 ctatgtgggc gaagacaacg agcgtaaggt accggtaatg attcaccgcg caattctggg
     2161 gtcgatggaa cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg
     2221 gcttgcgcca gttcaggttg ttatcatgaa tattaccgat tcacagtctg aatacgttaa
     2281 cgaattgacg caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga
     2341 aaagattggc tttaaaatcc gcgagcacac tttgcgtcgc gtcccatata tgctggtctg
     2401 tggtgataaa gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct
     2461 gggaagcatg gacgtaaatg aagtgatcga gaagttgcaa caagagattc gcagccgcag
     2521 tcttaaacaa ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc
     2581 taaccgtatc aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga
     2641 gcagcttggt attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga
     2701 cttagtcgag atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa
     2761 attcctctat gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca
     2821 ggttaaggaa attaaattcc gtcctagtac agatgaaggc gactatcagg taaaactccg
     2881 cagcctgatt cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgcgg
     2941 tcgtgagatg gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt
     3001 gcaagaactg gcagtggtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat
     3061 ggtgctcgct cctaagaaga aacagtaagg ccttcaagta gcactgtctg tggagccttc
     3121 gggcttcaca ggctttgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat
     3181 taaaatgcca aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa
     3241 aggtggtttt aagcacaagc acgctaacct gcgtcacatt ctgaccaaaa aagcgaccaa
     3301 acgtaaacgt cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat
     3361 cgcgtgcctg ccgtacgcat aagccgttaa cgtttttaac tttttaatta gaatatagat
     3421 acaggagagc acatatggct cgcgtaaaac gtggtgttat tgcacgcgca cgtcacaaga
     3481 aaattttgaa acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac cgcgttgcct
     3541 tccaggctgt tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa cgtaagcgtc
     3601 agttccgtca actgtggatt gcacgtatca acgcagcagc acgtcagaac ggtatttctt
     3661 acagcaaatt catcaatggc ctgaaaaaag cctctgttga aatcgaccgt aagatcctgg
     3721 ctgatatcgc agtattcgac aaagtagcgt tcaccgctct ggttgaaaaa gcgaaagcag
     3781 ctctggcata agccagttga aagagggagc tagtctccct cttttcgttt caacgccatc
     3841 aaaacattga cttttatcgc cgtagccttt tcaataaagg tcttttgaag agtaccaaaa
     3901 ggtaacgcaa gcaatgaatg ctgctatttt ccgcttcttt ttttacttta gcacctgaat
     3961 ccaggaggct agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc tcccagtgga
     4021 ggcttttttt gtatgcgcgt tttgaaaaat aaaaagccag tctatcaggc gtaattgtcg
     4081 cagccagtct ggatacggcc agcgcggaga aaccggagcg tatttaagta cgtgagaatt
     4141 tcgagcactg cccgggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt
     4201 ccaacgaacc agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact
     4261 ggttgccagt gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt
     4321 gcgcgtcgaa tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga
     4381 gctgccgcca gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt
     4441 tcagcaggcg ctgaatgcgc gtaaagcgga actggaaagc gcagcgctga atgcgcgtct
     4501 ggcggcggaa acgattgatg tctccctgcc aggtcgtcgc attgaaaacg gcggtctgca
     4561 tccggttacc cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt
     4621 ggcaaccggg ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg
     4681 tcaccacccg gcgcgcgctg accacgactc tttctggttt gacactactc gcctgctgcg
     4741 tacccagacc tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat
     4801 catcgcgcct ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca
     4861 tcagatggaa ggtctgattg ttgataccaa catcagcttt accaacctga aaggcactct
     4921 gcacgatttc ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta
     4981 cttcccgttc accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg gtaaatggct
     5041 ggaagtgctg ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc
     5101 ggaagtttac tctggtttcg ccttcgggat ggggatggag cgtctgacta tgttgcgtta
     5161 cggcgtcacc gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa
     5221 ataaggcagg aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg
     5281 gcgattgata gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga agttgacggt
     5341 gtagaaccgg ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg
     5401 cagcatccga acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg
     5461 ctggacatcg tctgtggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagctacc
     5521 attggtgctg ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg tggcgaaccg
     5581 tctgaaggga tgctgtgctc cttctctgaa ctgggaattt ctgacgatca cagcggcatt
     5641 atcgaactgc ctgcagatgc gccgattggc actgacatcc gcgaatacct gaaactcgat
     5701 gacaacacca tcgaaatcag cgtaacgcca aaccgtgccg actgcttagg tatcattggt
     5761 gttgcgcgtg acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga aatcgttccg
     5821 gttggtgcga ccatcgacga cacgttgccg attacagtcg aagcgcctga agcctgcccg
     5881 cgctatcttg gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg
     5941 aaagaaaaac tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac
     6001 tatgtactgc tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc
     6061 ggcattgtgg tgcggatggc aaaagagggc gaaacgctgg tgctgcttga tggcactgaa
     6121 gcgaagctga atgctgacac tctggtcatc gccgaccaca acaaggcgct ggcgatgggc
     6181 ggcatctttg gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cttgctggaa
     6241 tgcgctttct tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact
     6301 gatgcgtctc accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa
     6361 cgtgcgaccc gtctgctgat cgacatctgc ggtggtgagg ctggtccggt aattgatatc
     6421 accaacgaag caacgctgcc gaagcgtgca accatcactt tacgtcgtag caaactggat
     6481 tgcctgatcg gccatcatat tgctgatgaa caggtaactg acattctgcg tcgtctcggc
     6541 tgcgaagtga ccgaaggcaa agacgagtgg caggcagttg cgccgagctg gcgtttcgat
     6601 atggagattg aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc
     6661 ccggatgagc cggtacaggc aagcctgatt atgggtactc accgtgaagc tgacctgtcg
     6721 ctcaagcgcg tgaaagcgct gctcaacgac aaaggctatc aggaagtgat cacctacagc
     6781 ttcgttgatc cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca
     6841 agcccgatct ctgttgaaat gtcagcaatg cgtctttctc tgtggagcgg cctgctggca
     6901 accgtggtgt acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagcggtctg
     6961 cgtttcgtac cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt
     7021 gtgatttgcg gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc
     7081 tatgatttga aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt
     7141 gagttccgtg cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg
     7201 aaaggggaac gtattggttt tgttggagtt gttcatcctg aactggaacg taaactggat
     7261 cttaacggtc gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg
     7321 cctcaggcgc gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc
     7381 gcagaaaacg ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag
     7441 gtagttggcg taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag
     7501 agcctcgcca taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt
     7561 gccgctaccg tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg
     7621 gattgaacct atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg
     7681 gcttagcaag cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc
     7741 tctggaaaac ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa
     7801 gaatcaacgc ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg
     7861 cgtggtgacc ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa
     7921 agacgagtaa tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct ttcaataact
     7981 aattgattta taatgtttta tatctcttcg ttccacgatg cgtccacatt tagtgggggg
     8041 aggggagtaa gcccgctaca ttgtgggctt tgtcttatag tctgatcact gtttcaagtc
     8101 ggtgaggaca ccaaaaagaa accccgcagt ttttacgctg cgggtttgtt gttcatgtct
     8161 gtgagatagg gtgccttatc gaccttaccc tggcaaccga ttgacggggg attgctcccc
     8221 cgtcgcggtt tccttactgc ttacactgta agaacgccgc aaactccgct ccccagaagc
     8281 tcatccgtat ttcgcacagc gaaccgtgca gcatccagat gatgaggatt gccgtcacgc
     8341 agaacgtgat ggccgtaagc gatttttgcg acatagcgct tgctcctttt tcggggaggc
     8401 gctaacctat cacttgctta gggtagacgg ttagggcctc ggttaaacaa aaatgttttc
     8461 cggggccttt ccacatctgg ccttcaggta ttccctccga ccatcagccg aaaggcaccc
     8521 gcgcgtaatc tatcgctttt ttgttactcc ggcaattctg ctcgttaatt ctgaggtaag
     8581 ggcaaactca tctgattgtt tcttctgtgt gaagttggca gttcatgcca
//
LOCUS       DAJTMK010000155         8627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTMK010000155 DAJTMK010000000
VERSION     DAJTMK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.159"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            77..1720
                     /gene="eptA"
                     /locus_tag="OO135_003270"
     CDS             77..1720
                     /gene="eptA"
                     /locus_tag="OO135_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5399012.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            1717..2385
                     /gene="basR"
                     /locus_tag="OO135_003271"
     CDS             1717..2385
                     /gene="basR"
                     /locus_tag="OO135_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5399013.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2386..3486
                     /gene="pmrB"
                     /locus_tag="OO135_003272"
     CDS             2386..3486
                     /gene="pmrB"
                     /locus_tag="OO135_003272"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5399014.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRARVRLKKDQNVANQI"
     gene            complement(3462..3551)
                     /gene="pmrR"
                     /locus_tag="OO135_003273"
     CDS             complement(3462..3551)
                     /gene="pmrR"
                     /locus_tag="OO135_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5399015.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(3663..5165)
                     /gene="proP"
                     /locus_tag="OO135_003274"
     CDS             complement(3663..5165)
                     /gene="proP"
                     /locus_tag="OO135_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5399016.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5430..6308)
                     /locus_tag="OO135_003275"
     CDS             complement(5430..6308)
                     /locus_tag="OO135_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5399017.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(6305..8533)
                     /gene="crfC"
                     /locus_tag="OO135_003276"
     CDS             complement(6305..8533)
                     /gene="crfC"
                     /locus_tag="OO135_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5399018.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSQPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTFCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTKLQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2203 a         2290 c         2126 g         2008 t
ORIGIN      
        1 ccaaccttaa gaacttaagg ttggcttaat tttgctttgc gagcatatgc gcactttgtt
       61 cgatggaaac accgtgatgt tgaagcgcct actaaaaaga ccctctttga atttactcgc
      121 ctggctattg ttggccgctt tttatatctc tatctgcctg aatattgcct tttttaaaca
      181 ggtgttgcag gcgctgccgc tggattcgct gcataacgta ctggttttct tgtcgatgcc
      241 ggtcgtcgct ttcagcgtga ttaatattgt cctgacacta agctctttct tatggcttaa
      301 tcgaccactg gcctgcctgt ttattctggt tggcgcggct gcacaatatt tcataatgac
      361 ttacggcatc gtcatcgacc gctcgatgat tgccaatatt attgatacca ctccggcaga
      421 aagttatgcg ctgatgacac cgcaaatgtt attaacgctg ggattcagcg gcgtgcttgc
      481 tgcgctgatt gcctgctgga taaaaatcaa acctgccacc tcgcgtctac gcagtgttct
      541 tttccgtgga gccaatattc tggtttctgt actactgatt ttgctggtcg ccgcactgtt
      601 ttataaagac tacgcctcgt tgttccgcaa taacaaagag ctggtgaaat ccttaagccc
      661 ctctaacagc attgttgcca gctggtcatg gtactcccat cagcgactgg caaatctacc
      721 gctggtgcga attggtgaag acgcccaccg caacccgtta atgcagaacg aaaaacgtaa
      781 aaatttgacc atcctgattg tcggcgaaac ctcgcgggcg gagaacttct ccctcaacgg
      841 ctacccgcgt gaaactaacc cgcggctggc gaaagataac gtggtctatt tccctaatac
      901 cgcatcttgc ggcacggcaa cggcagtttc agtaccgtgc atgttctcgg atatgccgcg
      961 tgagcattac aaagaagagc tggcacagca ccaggaaggc gtgctggata tcattcagcg
     1021 agcgggcatc aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggtg cctgcgaccg
     1081 cgtgcctcac cagaacgtca ccgcgctgaa tctgcctggt cagtgcatca acggcgaatg
     1141 ctatgacgaa gtgctgttcc acgggctgga agagtacatc aataacctgc agggtgatgg
     1201 cgtgattgtc ttacacacca tcggcagcca cggtccgacc tattacaacc gctatccgcc
     1261 gcagttcagg aaatttaccc caacctgcga caccaatgag atccagacct gtaccaaaga
     1321 gcaactggtg aacacttacg acaacacgct ggtttacgtc gactatattg ttgataaagc
     1381 gattaatctg ctgaaagaac atcaggataa atttaccacc agcctggttt atctttctga
     1441 ccacggtgaa tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt atgccatcgc
     1501 ccctgatagc caaaaacagg tgccgatgct gctgtggctg tcggaggatt atcaaaaacg
     1561 gtatcaggtt gaccagaact gcctgcaaaa acaggcgcaa acgcaacact attcacaaga
     1621 caatttattc tccacgctat tgggattaac tggcgttgag acgaagtatt accaggctgc
     1681 ggatgatatt ctgcaaactt gcaggagagt gagtaaatga aaattctgat tgttgaagac
     1741 gatacgctgt tattgcaggg actgattctg gcggcgcaaa ccgaaggcta cgcgtgcgat
     1801 ggcgtgacaa ccgcgcggat ggcggaacaa agccttgaag ccggtcatta cagcctggtg
     1861 gtactggatt tagggttacc cgacgaagat ggactgcatt ttctcgcccg tatccggcag
     1921 aaaaaatata ccctgccggt actgatcctc accgctcgcg atacgctgac cgacaaaatc
     1981 gccgggctgg atgtcggtgc cgatgactat ctggtgaagc cttttgcgct ggaagagtta
     2041 cacgcccgta tccgcgccct gctacgacgc cataataatc agggcgaaag tgagctgatt
     2101 gttggcaatc tgacgctgaa tatgggtcgc cgtcaggtat ggatgggcgg tgaagagttg
     2161 attctgacgc ccaaagaata tgctctgctg tcacggttaa tgctcaaagc aggcagtccg
     2221 gtacatcggg aaattctcta caacgacatc tataactggg acaatgaacc ctcgaccaac
     2281 accctggaag tgcatatcca caatctgcgc gacaaagtgg gcaaagcccg tatccgcacc
     2341 gtgcgcggct ttggctatat gctggtcgcg aatgaggaaa actaattgaa tctgatgcgt
     2401 tttctgcgcc gaccaatatc gctgcgccaa cggctgatat tgactatcgg ggccattttg
     2461 ttggtgtttg agctgatcag cgtcttctgg ctatggcatg aaagtaccga gcagattcag
     2521 ctgtttgagc aggcgctacg cgacaatcgc aacaacgatc gccacatcat gcgtgagatc
     2581 cgcgaggcgg tcgccagcct gattgtcccc ggcgtcttta tggtcagcct gacgctattt
     2641 atctgctatc aggcggtacg ccgcatcacc cgcccgctgg cggagctgca aaaagagctg
     2701 gaagcgcgca ctgccgacaa cttaacgccc attgccattc acagcgccac cctcgaaatc
     2761 gaagcggtgg tttcggcgtt gaacgatctg gtcagtcgcc tgaccagcac gctggataac
     2821 gaaaggttgt ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc gggggtgcgt
     2881 ttgcatctgg aactgctggc gaaaacgcat cacattgatg tagcaccgtt agtggcacgg
     2941 cttgatcaga tgatggagag cgtctcccag ctgctgcaac tggcgcgtgc cggacagtca
     3001 ttttcttccg gtaattatca acatgtaaaa ctgctggaag atgtcattct cccctcgtat
     3061 gacgaactca gtaccatgct cgaccagcga cagcaaaccc tgctattgcc agagagcgcc
     3121 gccgacatca ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg aaacctggta
     3181 gaaaacgccc atcgttacag cccgcaaggc agcaacatta tgattaaact gcaagaagat
     3241 ggcggagcgg tcatggcagt tgaagatgaa ggaccaggta ttgatgagag taaatgcggg
     3301 gagttgagta aagcgtttgt acgtatggac agccgttatg gcgggattgg tctggggtta
     3361 agtattgtca gccgcattac acagttgcat cacgggcagt ttttcctgca aaaccggcaa
     3421 gagacttccg gcacgcgggc cagggtacgg ctgaagaaag atcagaacgt ggcaaaccag
     3481 atataaagaa agctgctgac caccagcacg ctgaacacgg tagttaaact ttcataaaca
     3541 cggtttttca ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt cagagattaa
     3601 gtgaacctta agagttcaat tggcctgata agacagcgtc acatcaggcc atccgtttca
     3661 gcttattcat caattcgcgg atgttgctgc accaggcggg tacgtttcgc ctgcaaatcg
     3721 gcaatctcgt ggtcaatatt atcgattttc tgctcgatat tatcgtaatg ctcgacgaga
     3781 atttccttcg cttcctgtat atctgacgcc gccggtgtcg cacctttcaa cggacgattt
     3841 gccgtctctt tcatggttac gccggtgatt aaaccaatca ccgccactac catcaggtaa
     3901 taggcaggca tcatcagatt ctgcgagctt tcgaccagcc aggccgccag tgttggcgtc
     3961 agaccggcaa ccagcaccga aatattaaat gccgccgcca gcgcgctgta acggatatgc
     4021 gtcgggaaca tcgctggcaa ggtagaagcc ataacgcccg taaagcagtt aaggatcacc
     4081 gccagcatca gtaacccggc aaaaatcagg ccgatgacgt tactgttaat cagaataaac
     4141 gccgggatcg ccaacacaaa cagggcaaca ctaccaagta gcacaaacgg acgacggcca
     4201 aaacggtcac tcagcaagcc catcaccggc tggacaaaca gcataccgat cataatggcg
     4261 ataataatca gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa ataactcggc
     4321 atataggtca gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc aatacatgtc
     4381 aacaggctgc gccagtattt agtggcaatc tctttaaacg agactttcgg gccatcctgc
     4441 aaaccttcgc ggtcgccctg ttccagttta tcaacatgct gctggaacgc cggagtttct
     4501 tccagcgcat ggcgcaggta aagcccgata atccctaacg gcagagcaat aaagaacgga
     4561 atacgccagc cccagtcgag gaagttcgct tcgccgacaa tggtcgaaat taacaccacc
     4621 acgcccgcac ccagcacaaa cccggcaata gaaccgaagt ccagccagct gcccataaag
     4681 ccacgtttac ggtcagggga gtattccgca acaaatatcg acgccccggt atattcaccg
     4741 ccgaccgaga aaccttgtgc catcttacag atcaacagca gaatcggtgc ccaaatacca
     4801 atcgtgtcgt aggacggtat taagccaata cagaacgtac tgatcgacat aatcacaata
     4861 gtgatagcga ggatcttctg gcgaccatat ttatcgccca acataccaaa gaagagtccg
     4921 ccaagcggtc gaatcagaaa gggaacggag aaagtggcaa gtgcagcaac catctgcacg
     4981 ctggggtcag cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa accataaaca
     5041 ccaaaatcga accattccat tgcattaccc agtgatgctg cggtaatggc tttacgcagt
     5101 ttactgtcat taataatggt gacatcacga agggtaatcg gttttacttt tttccttttc
     5161 agcatagctt tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa tcagcgcgac
     5221 ggccgcaaaa ggggagcggg aattgcagga tgcaatctct gtaacaagcg tagcgggttt
     5281 gcttacaccc tccggttaag gaatgtacaa tcaaaccagt tgacgcctga attgggcagt
     5341 taatgacctt tttaccctac cagtgtttaa atttgtgatc aacttcacac atggatttcg
     5401 tttaggacat cattgatgta actgattatt tacgccaaat ttacagagaa gatttcctcg
     5461 accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg gcatccccag cgcgacagtt
     5521 tgccaacgtt gggtcactga ccagcaaacg ggttgtttat ctgccgtggt ccactttccc
     5581 agccaactta aaaaatcagc atgatccccg cccgtcggaa cggtcgaaga ggcaaaccac
     5641 tgatggtaat agtgacgcgt ggtcggggcc gcattgatac tctctgccag atgttgtgtc
     5701 gctttcacgc gcaaatcacc cagcagcaac gccaccgctt gcgggcttcc cagacgacag
     5761 gcatcgccaa atgcccccca acgcaactct tcgagaacga taaaacagcg cccaggcaac
     5821 gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa tttgctcgct ctgatcacct
     5881 gcctgcacca gtaggcctcg ttgcgttaac gccttttcct tccagcgaca acgggctgaa
     5941 aaccactcat acaaggcggc aatttgctgg gggagctccg ggaaagcaga gtgctggcgg
     6001 tgatccattt tttgcagcag cgccagatgc tgcgcgatct gcgtcagcgc cagatgaccg
     6061 ggatccagca tcgacgccag tttttccacc attgtcagtc gcaggctaag cggcgcattt
     6121 aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg aaagaagttc ctgcatcagt
     6181 cgttcacgaa actgctgctg atgggcttgt ggaaggcggg catcatcgcc ctgcgccaga
     6241 tccactaaaa agcggggata aaccgactcc agcacgcgac cggggccgtc cagtaacgtc
     6301 ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc atcgcgtaac agctgggcgt
     6361 cggtgtaaat ccagccgtga gtcatcacag tctgctgcaa ttgctgctgc atcagcctga
     6421 ccaccgattc attttgttga cgcagagcca ggctttcgcg taaacgcgtc tgtaatttcg
     6481 tcaaacatga agcgaactca gcgaaaaaag tattcatgcc tgccgtaaca gattcatcga
     6541 cctgctctgc cagaacttta gccatttgtt ggcaataaag atcgacttct gcgcttaatg
     6601 ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga tttctcattt ccccgtcccc
     6661 agtcgggctg attcaaccag cgcgaaaaag tctcacgcac aacgcctaaa cgcgtgctct
     6721 gctcgtccgt tgcctcctgg cgcgaaatga cttcactgaa cagctggcga gtgttgacgt
     6781 ggggaactac tccgtgaaaa acaggaaaat gaaacccagg acgaaaccct gactcgctca
     6841 attccatttt gacttgttgc tcaatgggcc gaataacatc ggttaacacc cggcaaaagg
     6901 tggattcaag ctcggcaaaa cgcagcgtaa agtcgcgact gatggtgttc tgcgccgtct
     6961 gtaacagtgt ctcacagcag gtacgaatct cgcttaacgg ctgcgaatca tcctgaaaca
     7021 aggcggctaa ctgcgcattc agcgcatctt gctgttgacg cagaaagtgg ttggcggagg
     7081 tcagggccag ctcgatttca tgtttaatct cgccgctcac ctgcgcctga ttgagttgca
     7141 atagctgcaa actttcttcg acctgatgga tattttgccg caattgttca caagcgacgt
     7201 ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg ctgcgcgtaa ttcaacagtt
     7261 tatgcgcagc agatcgcaga gcatacaatg aggcgttagc gtaagcggca tgaagcaacg
     7321 cctgaattgg ctgggcgaac agcgaatctt cccacaactg atcggcagca tgacgaatat
     7381 gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc gagcgcggca tgggcaaaat
     7441 cttccaccca gcgttgttgc tctggcgctg gtaacttacc gttgttggct aactcatagc
     7501 gcgcccgatt cgccaggtag ccccacatcg acgacaccgg aaatatctgc tgtggcgtaa
     7561 tacagccttt catcagcgtc ccggaaatca gtgcccgcac ctggtcggcg tcgtcactgt
     7621 tacgatcctg ttgatcgaac ttattgacca gcacatacag cggcaccgat tgccccaccg
     7681 ccaaaatcgc ctcacggacc tcttcatcag agatcgattt cagttgcgta taatccagca
     7741 ccgccagtac cgccgaggcg cgtgccagct gctggttaag cattttttgc agatgcggtt
     7801 gcccggcttc atttggcccg ggggtatcca gtaacgtcaa ctgaccggga taactttcca
     7861 gccccgccag atggacaaac tccacttcaa tcacgggaat atgctcaatg gcggcgtaag
     7921 cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg cactaaatca ttcaaacttt
     7981 tcagacaatg aaaaataggc tgggcaccca gataatattt ttcgaaagcg acgccatttt
     8041 cgatccgctg cataagcgca cgcatatctt tatctatttc cagcacatcg gtcagatgct
     8101 taatatcgca atcacgcagg cgctgttgta attgttgaat taaacaatcg attggcgcga
     8161 catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt atggcgaata agcgtcggca
     8221 gcgcagtcat tgggcgatta cgattaggca gaacctccgt accaacaatg gcattaatgg
     8281 tggttgattt ccctgctttc atagtaccga caatcgccag caccatttcc agtcgggaaa
     8341 ttttacgcaa ctcattattc agcatcgcgt aacgttcggc gatattaggc tgactccagg
     8401 gtaaagccag ttgtgccgca tcgtctccgg gtacagagag aggcattttt tccagtaact
     8461 gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc ctgacttaac tcatacaggg
     8521 tctgtgtgta catagaaaat tcttccttaa agcaaatttt gttactttta tttagccaga
     8581 ttgtttttga gttctgtttt cggcttttat aattactgca agaaata
//
LOCUS       DAJTMK010000156         8600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTMK010000156 DAJTMK010000000
VERSION     DAJTMK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.160"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..182
                     /locus_tag="OO135_003277"
     CDS             <1..182
                     /locus_tag="OO135_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5399019.1"
                     /translation="AIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQK
                     GEVVGVDDQCKEHKE"
     gene            401..643
                     /locus_tag="OO135_003278"
     CDS             401..643
                     /locus_tag="OO135_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399020.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(973..1902)
                     /gene="fdhE"
                     /locus_tag="OO135_003279"
     CDS             complement(973..1902)
                     /gene="fdhE"
                     /locus_tag="OO135_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5399021.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1899..2534)
                     /gene="fdoI"
                     /locus_tag="OO135_003280"
     CDS             complement(1899..2534)
                     /gene="fdoI"
                     /locus_tag="OO135_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5399022.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMSTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2531..3433)
                     /gene="fdoH"
                     /locus_tag="OO135_003281"
     CDS             complement(2531..3433)
                     /gene="fdoH"
                     /locus_tag="OO135_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5399023.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3446..6496)
                     /gene="fdnG"
                     /locus_tag="OO135_003282"
     CDS             complement(3446..6496)
                     /gene="fdnG"
                     /locus_tag="OO135_003282"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5909..5911),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5399024.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6690..7523
                     /gene="fdhD"
                     /locus_tag="OO135_003283"
     CDS             6690..7523
                     /gene="fdhD"
                     /locus_tag="OO135_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5399025.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7677..8600
                     /locus_tag="OO135_003284"
                     /pseudo
     CDS             7677..8600
                     /locus_tag="OO135_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         2048 a         2180 c         2271 g         2101 t
ORIGIN      
        1 aggcaattaa acggctgaac aagcttcggg gctggcgtaa ggtttcacga tctgcaattt
       61 tacgcgaagc ggttgagcaa tatctggagc ggcagcaatt tccggtgcgt aaagcaaagg
      121 gaggcaggca aaagggggag gtagttggtg ttgatgatca gtgtaaggag cataaggaat
      181 gaaaaaaatt tttcattctt gaatataaaa aaacagatgc ccttattctg gtattaatac
      241 aaggctgttt tacttgaact tataataact gcaactgtta catcgtatct ggaaaacgcc
      301 tcgcaaaaat aaaaaatgat gcgtaaatga gctttcttta aagattcggc aagggtactt
      361 ttctctccat agattcaaaa atgatattgg cgagatattt atgaactctc tggcaggtat
      421 agatatgggc agaattttac tcgatttatc gaatgaggtg attaagcaac ttgatgatct
      481 cgaggtgcag cgtaatcttc ctcgtgcgga cctattaagg gaagcggtag atcaatacct
      541 gataaatcaa tcgcaaacag caagaaccag tgttcctggc atctggcaag ggtgtgagga
      601 agatggtgtc gaatatcagc gtaagctgcg cgaggaatgg taatgggaaa aaatgatgtc
      661 aatcagattg ctgacaacgt gcgcgttgtt catgccggat gcggcgtgaa cgccttatcc
      721 ggcctacaaa gtcatgcaaa ttcaatatat tgaagtttat tggtaggcct gataagcgca
      781 gcgcatcagg caatcatacg cttgtcatca gtgacatacc cataacctta tcgatgtgtt
      841 ggagggaaga acgaggcaaa cagacaattg cggcatgatc ttgaatgggt aacgcggaat
      901 acgagagatg gtgtcgtcac accctatgag ctgtaatccg ggcgaccagc gccacccgga
      961 aaacgtcaga gattactccc cttcacccgg aaacaggaac gggttgatgg aactgcgggc
     1021 atagccttct tgctccattc gcgcgtccag taccagagaa gcgaggtcat ctgccacggc
     1081 ttcaactttc ggttcttttt cctgataaag aatcttcagg taagtgccgc agtcatcgca
     1141 gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca gtttgccgct
     1201 ctgttcacag ttgctgcatt ttacgcgcac tacgtgccat tcggtttcac acaggttgca
     1261 gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc atgctggaca ccggcataga
     1321 accacaaacc gggcaatatt gacgttgctc gccgtattca gcgcgggctt tgccggggat
     1381 cagattggcc atctgcgccc agtagagcga cagtgcagcc cagataaacg gcgctttatc
     1441 gctgctgacg gatgagaaat cagaggcaaa cagtgcgctg gccatatctt ccagctcctg
     1501 agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca tttcaggttt
     1561 cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg
     1621 aatatccagc gggggcttgc cctgtgcgct ggcttcttta atgcgcgcgg tcagatccat
     1681 ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg
     1741 caggtaatca tccagcggat tattttctgc cagctcgcgc agacgctcgg cgcggcggtt
     1801 gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg tacgtttctc
     1861 gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt cttttccgtt
     1921 gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc
     1981 catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc
     2041 atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc gaatcggatc
     2101 accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc gctcaccagc
     2161 agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa gttataacgc
     2221 ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc ccaaaagata
     2281 tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat gaacgaggca
     2341 aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt gctcatgatt
     2401 tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg
     2461 aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg cacgatggtg
     2521 tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc tcttcctcat
     2581 ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggctgcgaag gtagccgcaa
     2641 agccaacagc tgcgagcggt ttccagatgc ctttccagaa tttcacggtt tcgctgattt
     2701 ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc agcacgtaca
     2761 taacgtgtgt accaccgacg cctgccggat cgtacaggcc cgcattgtcg taaccgcggg
     2821 ttttcagctc cgccacgcgc tcgctcgcca gcgttttcat cgactctttc gtaccgaagt
     2881 gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc ttgcccaacc accacgcggt
     2941 caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga
     3001 acggacagcc cgcaatgcaa taaccgcagc caatgcactg ctcggactgg aagtcgacga
     3061 taccgttggc atactgaata attgcacctt ccgccgggca cgctttcaga cagcctggat
     3121 cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg ttctgctcca
     3181 cttccgagaa gcgcatcacc gtccacgatt tggcacttaa atcattgggg ttgtcgtaca
     3241 ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac
     3301 aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct tcctggaagt
     3361 cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg atatcttgag
     3421 attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg aaggacttaa
     3481 actccggcgt ctgcgtgttc gcatcaccaa cgaatggcgt taaagtattg gcaataaagc
     3541 cttttttagc tacgccttca tagccccagt gaatcggaat accgatggta tcgatatctt
     3601 tgccgttcgc tttcagcgtg cgaatacgtt tggtcaccac cgctttggct ttgatatagc
     3661 cacggttgga ggagactttc acggtatcgc cctgggcaat gccaagttta ttcgccagcg
     3721 actccccgat ttccacaaac tgctctggct gcaaaatcgc gttcaacagc gcgtgtttgg
     3781 tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg aacttatcgg
     3841 ctttacccaa tgcttcggcg tcgtctttaa agatacgtgc agccgggttc gagataacgt
     3901 ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg
     3961 gaccttccgc catcttatcg agggcaaaca gacgccccat gccttcctgc tgcatgataa
     4021 acggcccgac gccgctaccc ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc
     4081 acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta ccctgcggat
     4141 ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat gcccagccca
     4201 gcgtgttacc gaggccagac ggatcggcgt tatcacgacg cgccatctgg ttgccttccg
     4261 gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtaccgtca tcgcgcaatt
     4321 gggcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt gccggatcgg
     4381 taatatcggc cagcgcctta ccgttgctct ccatcgctac ttcttccgac ttcggctcat
     4441 gcggaatggc gtagttccag gtcatgttca gcacctgatc cgggttcgcg ccaccctgtt
     4501 cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcaccg tcagtcagcg
     4561 caatccccgg ggcgtccgca cctttccagt gccactgcaa ccagcgacct gagttaacga
     4621 ttgaaccgtt ctcttctgcg aagcaggtcg atggcagacg gaacacttcg gtctggatct
     4681 tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag gtttcagtgt
     4741 tcagcgggtc gatggtgacg aggaacttca gtttcgacag acaaccgatc actttgtttt
     4801 tgttcgggaa tgaggcaaca gggttaaaac cctggcagat atagccattg accttgccct
     4861 ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc ggcaaccagt
     4921 caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc atcatagaga
     4981 cgaagaattt cgggtagttg ccccagtagt ttacctggcc ttccagcagc ggttttggcg
     5041 tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac
     5101 ctggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg gagtgaccgc
     5161 gcagggcgtt aacgccgccg cctgccatcc ccatattgcc gagcagcagc tggatcatcg
     5221 ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt ccagccgagg gcatacagga
     5281 acgaggcagt tttatcgtga gcactggttt ctgcgatgta ttcgcagact ttcaggaacg
     5341 cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct
     5401 gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt ttggcgaagc
     5461 cgttttcgtc cagttcgtaa gtccaggagg atttatcgta cttgcgtttt tccgcgtcat
     5521 agccggtgaa caggccatct tcaaagccgt aatcctcacg cacgatcagg ctggcgttgg
     5581 tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac agcaatacgc
     5641 ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca gctaccgccg
     5701 ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccgttgtga attttggctt
     5761 ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc atcaccacga
     5821 cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg
     5881 gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca
     5941 tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc gacgcggaag
     6001 cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg ttttgcgcaa
     6061 tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac gcctcttccc
     6121 aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac ttcagacggc
     6181 tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca ccgcggttga
     6241 ccgggtgatc cggatcgcct tcgatatgga agatagatgc tttggcgttt tttgctccgt
     6301 caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag gtattacggg
     6361 tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagtgctacg ctgggtgcaa
     6421 aacctagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta aagaactgcc
     6481 ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca tttacattcg
     6541 cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc
     6601 agaattggct caatttccct ccatccagga agggtttgta acagaatacc ataatgttgg
     6661 tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcgaaaa gaaatcgaaa
     6721 atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat gatttacaac
     6781 acccacggtt agatgaggtc gcggaagaag ttcccgttgc gctggtctac aacggcattt
     6841 cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc ggtttttcgc
     6901 tttccgaggg gattatcgaa agtccgcgcg atatcttcgg catggatgtc gttccttcct
     6961 gtaatggtct tgaagtacaa attgagcttt ccagccgccg ctttatgggg ttgaaggagc
     7021 gccgccgggc gctggcggga cgtacgggct gtggcgtatg cggcgtggag caacttaatg
     7081 acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc aacaaactgg
     7141 atgatgcatt acgtcatctc aacgattttc agccagtggg gcaactgact ggttgtactc
     7201 acgccgctgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc
     7261 gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa agctggcagc
     7321 aaggtgcggt actggtttcc agtcgcgcca gttatgaaat ggtgcaaaag tcggcgatgt
     7381 gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac gcttgctgta gaagtggctg
     7441 agcgctgtaa tctgacactg gtaggttttt gtaaaccggg tcgggcaacg gtttataccc
     7501 atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatatatta aattgcagga
     7561 cttattcatt tcgtgaattt tattattttt atttataaat aaaacgcgtg tagtggcgtt
     7621 ttcttttttc atttattatg ttcactctct tatctatata tttataaggc aattaaatga
     7681 aaaggaattt attatcttcc gctattatag tcgccatcat ggccctcggt ctgacgggtt
     7741 gtgatgataa aaaagccgaa acagaaacgc ccccgcctgc caatagtcaa cctgccgcac
     7801 cagctcctga agctcccgtt gcaaaagcag aagctaaacc tgaaacacct gcgcaaccgg
     7861 tggtcgatga acaagcggtt ttcgacgaaa aaatggatgt ctatatcaag tgctataaca
     7921 agttacagat ccctgtacag cgcagtctgg cgcgttatgt tgactggctg aaagatttta
     7981 aacaggggcc taccggtgaa gagcgtactg tttatggcat ctacggcatt agtgaatcca
     8041 acctcgctga gtgtgaaaaa ggcgtaaaaa gtgctgtggc gttaacgcct gcgctgcaac
     8101 caattgatgg cgtggcggtg agttatatcg atgctgccgt ggcgttgggt aataccatta
     8161 acgaaatgga taaatattac acccaggaaa attataaaga cgatgcgttt gcgaaaggga
     8221 aaacgctgca ccagacattc ttaaaaaatc tgtaagcctt tgaacctgta gcagaatctt
     8281 atcatgcggc gattcaggaa attaacgata agcgccagct tgcggaactg aaaaatattg
     8341 aagaaagaga agggaaaata ttccactact attctctggc agtcatgatt tcagcgaaac
     8401 aaattaataa cctgatatcg caagagaagt ttgatgttga tgcggcaatg aagaaagtgt
     8461 ctgaactgga aacgctggtg gcgcaggcca aagaagcgga taaaggcggc atgaatttct
     8521 cgtttattaa ttcggcaggc cagtatcaac tcgaggctaa aaaatacgtt cgccgcatca
     8581 gagataaagt cccgtactct
//
LOCUS       DAJTMK010000157         8562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTMK010000157 DAJTMK010000000
VERSION     DAJTMK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.161"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            198..908
                     /locus_tag="OO135_003285"
     CDS             198..908
                     /locus_tag="OO135_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5399026.1"
                     /translation="MATGKSFSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(958..1932)
                     /gene="ghrB"
                     /locus_tag="OO135_003286"
     CDS             complement(958..1932)
                     /gene="ghrB"
                     /locus_tag="OO135_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5399027.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIVPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(2036..2623)
                     /locus_tag="OO135_003287"
     CDS             complement(2036..2623)
                     /locus_tag="OO135_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5399028.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDNIILNMPNNVTFDSSSATLKPA
                     GANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMRLSQQRADSVASALITQGVDAS
                     RIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            2776..5109
                     /gene="bisC"
                     /locus_tag="OO135_003288"
                     /pseudo
     CDS             2776..5109
                     /gene="bisC"
                     /locus_tag="OO135_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
     gene            complement(5078..5518)
                     /locus_tag="OO135_003289"
     CDS             complement(5078..5518)
                     /locus_tag="OO135_003289"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5399029.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(5515..6078)
                     /gene="tag"
                     /locus_tag="OO135_003290"
     CDS             complement(5515..6078)
                     /gene="tag"
                     /locus_tag="OO135_003290"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5399030.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFYQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            6236..6934
                     /locus_tag="OO135_003291"
     CDS             6236..6934
                     /locus_tag="OO135_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5399031.1"
                     /translation="MIIKKSGGRWQLCLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRIGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            7162..8364
                     /locus_tag="OO135_003292"
     CDS             7162..8364
                     /locus_tag="OO135_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5399032.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
     gene            complement(8502..>8562)
                     /locus_tag="OO135_003293"
     CDS             complement(8502..>8562)
                     /locus_tag="OO135_003293"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399033.1"
                     /translation="SVELHDKVIGHYLNIKHYQ"
BASE COUNT         1999 a         2096 c         2251 g         2216 t
ORIGIN      
        1 tctcgggcta caaaaaagca acggcaatat cattttttaa caatgaaaca acaaattaac
       61 cttgtttcat tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag
      121 cctgtcctgg actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg
      181 gtgaaaggag taagaaaatg gcgacaggaa agtccttctc tcgctggttt gcgcctcttg
      241 cggcgttatt aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta
      301 aagcgtttat cgacttcctg cagaatacag tgatgcgtag cggtgaacgt ctaccaaccc
      361 tgactgccga tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg
      421 gttattctca gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg
      481 ttaacgcaat tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga
      541 acggttcgct gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg
      601 cgcactctgc gctaaaacag accgatgatt tgaaaccggt ctttgatcag gcattcacca
      661 aagtggtaac gacgccagct gatgcattgc aaccgttgat tccggttgca caaactttca
      721 cgcaacaact ggtgatggtt ggagactaca ttgctcaaca aggtactcag gtgagctttg
      781 tagcaaatgg tatccagttc ccgacttcgc agcaggcaag tgaatataac aaactgattg
      841 cgccgttacc ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa
      901 cccaataaag agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcggctta
      961 gtccgcgacg tgcggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa
     1021 attatccacg gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg
     1081 cggtactgcg acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg
     1141 ttcgaagaca tccagcccgg cggcgtgaat ttcgcctttc tgcaatgctg cgatcagtgc
     1201 attttcgtca accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt
     1261 ggcgaattgt tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca
     1321 aacgaaatct gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc
     1381 ttctgcttct ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg
     1441 cgcacgttgt gccagcgcca tgccgatccg tcccatcccg acaatgccca gtgttttatg
     1501 gtgaacgtca gtgccgtacc agtccgggac tatgctcgcg gtccattcgc ctgcttttac
     1561 ccgttctgct acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc
     1621 ggcgacggtt tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag
     1681 cgcatcgaca tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg
     1741 cattttttcc agcaatgcgg catctacatt ctcgtttgaa cccagtaaac cttcagcttc
     1801 ggcaaaaatt gctgcatttt gttcgacggt ttgtgggctg aggtttgcca cctggtgaac
     1861 ggtgaaatgc tcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac
     1921 ggacggcttc atgcttctct ccattcactg ataaggcgct caccttagcc tgaaatatga
     1981 tgaaggaaaa ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca
     2041 gcgggcttaa ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc
     2101 tggcgattgg gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct
     2161 gggtgatcaa cgcgctggca acggaatccg cacgctgctg ggagagacgc atgttcaggt
     2221 cgtgaccccc cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt
     2281 tcagcaccat tgccacgccg gtcagggtgt tagcgcctgc cggtttcagg gtcgcgctgc
     2341 tgctgtcgaa ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta
     2401 cgctaacacc agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat
     2461 aacccacgcc gccgcccaga gctgcgccca cgagagagtc cagacctgcg ccgatagcag
     2521 atttgcctgc ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat accgccagag
     2581 cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat ccttaataac
     2641 ctttttattc tttgccacgg gttccgtggc gggagattat gccgcgtgaa catgaagatt
     2701 attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac atctaacctt
     2761 tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac tgccgcccac
     2821 tgggggccca tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg tggcgcgtta
     2881 gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca cagcaatacg
     2941 cgggtacgat ttccaatggt gcgcaaaggc tttctcgcgt caccggaaaa cccgcaaggc
     3001 attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga tcttattcac
     3061 caacaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc tggttcctac
     3121 ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg ctatatggcg
     3181 ctggcaggcg gttataccgg gcatctgggg gattattcga ccggtgcggc acaggcgatc
     3241 atgcagtatg tcgtgggtgg cagcgaagtt tatcaacagc aaaccagttg gccgctggtg
     3301 ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac gctgaaaatt
     3361 gcgtggaatg catctgatga gcaggggctt tcttactttt ccgcactgcg tgacagcggg
     3421 aaaaagctga tctgtattga tccaatgcga tcggaaaccg tcgatttctt tggcgataaa
     3481 atggagtggg tggcaccgca catgggcacc gatgttgcgc tgatgctggg gatcgcccat
     3541 acgctggtgg aaaatggttg gcacgacgaa gcgtttctgg cgcgttgcac cacaggttat
     3601 gccgtcttcg cctcttattt gctgggcgag agtgacggaa tagccaaaac cgccgaatgg
     3661 gcggcaggga tttgtggggt tcacgcagcg aaaattcgcg agttggcggc tcttttccac
     3721 caaaacacga ccatgctgat ggctggctgg ggaatgcagc gccaacagtt tggtgagcaa
     3781 aaacactgga tgatcgtcac gctggcggca atgttggggc aaatcggcac acccggcggc
     3841 ggttttggtc tttcttatca ttttgccaat ggtggtaatc ccacgcggcg ttctgcggtg
     3901 ctctcttcca tgcagggtag cttgccgggt ggcagcgatg cggtagataa aatccctgtt
     3961 gcccgtattg ttgaagcact ggaaaaccct ggctgcgcat accaacacaa tggcatgaat
     4021 cgcctcttcc cggatattcg ctttatctgg tgggcgggtg gcgccaactt cacccatcat
     4081 caggacacta atcgcctgat acgagcttgg caaaaaccgg agctggtagt tatctccgaa
     4141 tgcttctgga cggcggcggc aaaacacgcg gatatcgttc tgcctgcgac tacctctttt
     4201 gagcgtaatg atctcaccat gaccggcgat tacagtaatc agcatctggt gccgatgaag
     4261 caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc cgagttaagt
     4321 gaacgctggg agaagggcgg ttatgcacgt tttacggaag gaaaaagtga gttgcaatgg
     4381 ctggaaacgt tttataacgt tgcccgacag cgcggggcaa gccagcaggt tgaattgccg
     4441 ccatttgctg agttctggca agccaatcag ttaattgaga tgccggaaaa cccggacagc
     4501 gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc aggcgcatca attaaaaacc
     4561 gccagcggta agattgaaat cttctcgcag cgtattgccg attacgctta tccggattgc
     4621 ccggggcatc caatgtggct ggagccggac gaatagcagg gcaatgccga accggaacag
     4681 ttacaggtac tttctgctca tccggcgcat cgtctgcaca gccagctgaa ttacagttct
     4741 ctgcgcgaat tgtatgcggt ggcaaatcgt gagcctgtca ccattcatcc tgacgatgcc
     4801 ctggcgcgcg gcattcaaga tggcgatact gttcggctgt ggaacgcacg cgggcaaatt
     4861 cttgccggag cggtcattag cgagggaatt aaacctggcg tgatttgtat tcacgaaggg
     4921 gcatggccgg atttggattt aaccgctgac ggtatttgta aaaacggcgc agtcaacgtg
     4981 ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa tacggcgctg
     5041 gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga accaccggcc
     5101 agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg acaatgtgaa
     5161 aaccctgcgc ctggtaaaaa tttatcgccg gttgattttt ttgataaacc tccagcatca
     5221 ggtggggatg gcgctgctgc acatattgca tcagcgcctt accaataccg cgcctgacgg
     5281 ccttcggtgc gacaaacatc gctgccagaa atcggccttc cataatgctg acaaaaccga
     5341 gaagcttact gtcttcttcc cagacccagt tttgcgcatt ggcaagatac gcatcccgca
     5401 ccagcggaat gcaatcacgc cagtaattcg cttttataaa gggatgtccc caggttgtac
     5461 tttccagcca cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc cgaatcatgg
     5521 tttatttccc ggatagcaac agcagccaac cacatgatca ttcaccagcc cacatgcctg
     5581 cataaaggag taacagattg tggtaccgac aaacttaaaa ccacgttttt tcagtgcttt
     5641 agatagggcg tcggaggcgg gcgtagatgt ggggatttcg ctcaacgttg tggcttgtgt
     5701 cacctgtggc tgatgattta caaacgacca gacaaagtcg gcaaacggtt cgccgttctg
     5761 ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc cgcgatggcg
     5821 gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg ctgcgacttt
     5881 caccggatcg aactgataaa agcaggcgcg atagttttcg cgttttttga ggacggtgat
     5941 ccacgataac ccagcctgct gcccttcaag gcagatcatt tcgaacagtt ttttactgtc
     6001 agtttcaggc acgccccact cattatcatg gtaggcaata taaagcgggt cctgactcac
     6061 ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa tttcagccgc
     6121 ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat gcccgatatc
     6181 cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg tgttaatgat
     6241 cataaaaaaa agcggtggtc gctggcagct atgcctgctg gcgagcgtgg taatcagtgc
     6301 cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata cgcaacccgg
     6361 acattccaca gaacgttaca cctgggatag tgatcatcaa cctgattaca acgatatttt
     6421 gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc tggcggaaga
     6481 aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt tatatgaaca
     6541 ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct cgatgtataa
     6601 cgagtttggc gacagtacta ccacgctgac cgatccgtta tggcatgcca gcgtgagtag
     6661 cttaggctgg cgtgttgact cccggattgg cgatctccga ccctgggcgc aaatcagcta
     6721 taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc ggatgacggc
     6781 gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt tgctcaatca
     6841 aaatattgcc gcctatgccg cgctaactca ggcagaaaat accactaata atagcgacta
     6901 tctgtatacg atgggggtta gcgccagatt ttaacgtaac agtcacaatt gaaaccattg
     6961 aataacaata gttgtgctga tagtgggtgc taacttacaa ataataaatt tggtgaataa
     7021 ttgtcgcgtc attcattcct gaactaaggc atttcattcc gttctgatgg catttcatgc
     7081 cgtttttccc caggcataaa atgcacttcg ttatggttgt cggcagagat ttttcctttt
     7141 tattactgca ggaatactgc catgacacct tcaaattatc agcgtacccg ctggctgaca
     7201 ctcatcggta ctatcattac ccagtttgcg ctggggtcgg tttatacctg gagcctgttt
     7261 aatggcgcgc tttctgccaa gctggatgcg ccggtaagcc aggtcgcttt ctctttcggc
     7321 ttgttaagtc tggggctggc aatttcatct tctgttgcgg ggaaattaca ggaacgtttt
     7381 ggcgttaaac gcgtcactat ggcttccggc attttgctag gattaggctt cttcctgaca
     7441 gcgcattctg acaacctgat gatgctgtgg ttaagcgccg gtgtgctggt cggtctggcg
     7501 gatggcgcgg gttacctgct gacgctctct aactgcgtga agtggttccc ggagcgtaaa
     7561 ggtctgatct ccgcgttcgc tatcggttct tatggtcttg gtagcctggg tttcaaattt
     7621 atcgacacgc agctgctgga aacggtgggt ctggaaaaaa catttgtgat ttggggagcg
     7681 attgcgctgg tgatgattgt tttcggcgca acgttaatga aagacgcacc aaaacaggaa
     7741 gtgaaaacca gcaatggtgt ggtggagaaa gattacacgc tggcagagtc gatgcgtaaa
     7801 ccgcagtact ggatgttagc ggtaatgttc ctgactgcct gcatgagcgg cctgtacgtg
     7861 attggggtag cgaaagatat cgcccaaagt ctggcacacc ttgatgtggt ttccgcagcc
     7921 aatgcagtaa cggttatttc catcgctaac ctttcaggtc gtctggtgct gggcattctg
     7981 tctgacaaaa ttgcccgtat ccgtgttatc accattggtc aggtgatttc gctggtgggt
     8041 atggcggccc tgctgtttgc accattaaat gcagtgacgt tctttgcagc gattgcctgc
     8101 gtggcattta actttggcgg cactattacc gtctttccat cactggtcag tgagttcttc
     8161 ggcctcaata acctggcgaa aaactacggt gtgatttacc tcggtttcgg tatcggtagc
     8221 atttttggtt cgattatcgc ctcactgttt ggcggtttct atgtgacctt ctacgtgatt
     8281 ttcgccctgc tgattctgtc attggcgctt tctacgacga ttcgtcagcc agagcagaaa
     8341 atgttgcgtg aggcgcatat ttagaaatat gttgctactg aaataaatac gaggagggag
     8401 acttcaaagt ctcccttttt tatggacgca tattaaaaga acaataatat tcataccaca
     8461 aatatatttt atttatatat tatgtaggta atgactccaa cttactgata gtgttttatg
     8521 ttcagataat gcccgatgac cttgtcatgc agctccaccg at
//
LOCUS       DAJTMK010000158         8548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTMK010000158 DAJTMK010000000
VERSION     DAJTMK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.162"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..290
                     /gene="csgC"
                     /locus_tag="OO135_003294"
     CDS             <1..290
                     /gene="csgC"
                     /locus_tag="OO135_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5399034.1"
                     /translation="VSVKLRLPQYTIIPEVTLTQSCLCRVQILSLREGGSGQSQTKQE
                     KTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQWPPSSEKS"
     gene            411..722
                     /locus_tag="OO135_003295"
     CDS             411..722
                     /locus_tag="OO135_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5399035.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            818..1351
                     /gene="ymdB"
                     /locus_tag="OO135_003296"
     CDS             818..1351
                     /gene="ymdB"
                     /locus_tag="OO135_003296"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5399036.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1293..2774
                     /gene="clsC"
                     /locus_tag="OO135_003297"
     CDS             1293..2774
                     /gene="clsC"
                     /locus_tag="OO135_003297"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5399037.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPCSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2782..3939)
                     /gene="mdoC"
                     /locus_tag="OO135_003298"
     CDS             complement(2782..3939)
                     /gene="mdoC"
                     /locus_tag="OO135_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5399038.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4333..5868
                     /gene="mdoG"
                     /locus_tag="OO135_003299"
     CDS             4333..5868
                     /gene="mdoG"
                     /locus_tag="OO135_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5399039.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5861..8404
                     /gene="mdoH"
                     /locus_tag="OO135_003300"
     CDS             5861..8404
                     /gene="mdoH"
                     /locus_tag="OO135_003300"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5399040.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2157 a         2026 c         2226 g         2139 t
ORIGIN      
        1 gagtgtccgt taaactcagg ctacctcagt ataccattat tcctgaagtc actcttactc
       61 aatcttgtct gtgcagagta caaatattgt ccctgcgcga aggcggttca gggcaaagtc
      121 agacgaagca agaaaagacc ctctcattgc ctgctaatca acctattgct ttgacgaagt
      181 tgagtttaaa tatttccccg gaagatcggg tgaaaatagt tgttactgtt tctgatggac
      241 aggcacttca tttatcacaa caatggccgc cctcttcaga aaagtcttaa tttgttgaaa
      301 tgtcgagcat aagatgaatc tggagagaat ggtctgctgc gaatcagcca acctgaaagt
      361 atggataaca caaccctcaa ggatgactaa tcattgagga aatagaataa atgttcagac
      421 cttttttaaa ctctcttatg ctcggcagtt tgttttttcc ttttattgcc atcgcaggaa
      481 gcaccgcgca agggggcgtg atccattttt atggccaaat tgtggaaccg gcatgtgacg
      541 tcagcaccca gtcatcaccc gtagaaatga actgcccaca gaatggttct gttccgggaa
      601 aaacctattc cagcaaagcg ttaatgagcg gcaatgtcaa aaacgcgcaa gtagcctcag
      661 taaaagtgca gtatattgat aaacagaaaa agctggctgt aatgaacatc gaatataact
      721 aagtttctga acaacgcacg ttgctgagta gaaaaatgcg atttaaccaa aaagcctgct
      781 gtacacttaa gaaacaagaa ggtgttaagg aggcgttatg aaaacgcgta ttcatgttgt
      841 gcagggtgat attaccaaac tggccgttga tgtgattgtg aatgcggcta atccgtcatt
      901 aatgggaggc ggcggcgtcg atggggccat tcatcgcgca gcgggtccgg ccctgctgga
      961 tgcctgttta aaagtcaggc aacagcaggg cgattgccct acggggcatg ccgttattac
     1021 gcttgcaggc gatcttcccg ctaaagccgt agtacacacc gtcgggccag tctggcgtgg
     1081 tggtgaacaa aacgaagacc agcttttgca ggatgcctat ctcaatagtc tgcgactggt
     1141 ggcggcaaac agctatacgt cagtggcatt tcctgcaatc agtactgggg tttatggtta
     1201 ccctcgtgcg gcagcggctg aaatcgcagt aaaaaccgtt tcagaattta ttacccgtca
     1261 cgctttaccc gaacaggtat actttgtctg ttatgatgaa gaaaacgccc acctctacga
     1321 aagactcctt acccaacaag gagatgaatg atttgccccg gctggcgagc gcggtgctgc
     1381 cactgtgttc gcaacatccc ggtcagtgtg gtcttttccc tctggagaaa agtctggatg
     1441 cgtttgccgc ccggtatcgt ctggccgaaa tggcagagca tacgctggat gttcagtatt
     1501 acatctggca ggacgatatg tcgggtcggt tactgttttc cgccctgtta gccgcagcaa
     1561 agcgtggcgt tcgcgtccgt ttgttgctgg acgacaacaa tacgcccgga cttgacgata
     1621 ttttacgctt gcttgacagc catccccgca ttgaagtccg gctttttaat cctttctcgt
     1681 ttcgcttgct gcgtccgctt ggttatatca ccgacttttc ccgtctcaat cgccgtatgc
     1741 acaataaaag tttcactgtc gatggcgtgg ttaccttggt gggaggacga aatattggtg
     1801 atgcctattt tggggcaggg gaagagccac ttttttcgga tttagatgtt atggcaatag
     1861 gacccgtggt agaggacgtt gccgatgatt tcgcccgcta ctggtattgc aaatcggttt
     1921 cacccttaca gcaggtgctg gatgtcccgg aaggtgaaat ggcggatcgc atcgagttac
     1981 ccgcctcctg gcataacgat gccatgacgc atcgttactt acgccaaatg gaatccagtc
     2041 cgtttataaa tcatctggtt gatggaacgt tgccgcttat ctgggcgaaa acacgtttat
     2101 taagtgatga tccggcgaaa ggggagggca aggcaaaacg gcattcactg ttaccgcagc
     2161 gcctgttcga tatcatgggc tcacccagtg aacgcatcga tattatctct tcctattttg
     2221 taccgacacg cgcaggtgtg gcgcaactct tacggatggt gagaaaaggg gtaaagattg
     2281 cgatcctaac caattctctt gccgctaacg atgttgctgt cgtccatgcc ggatacgcgc
     2341 gctggcgcaa aaaattgctc cgctatggcg tggaattata tgaactcaag ccgacgcgtg
     2401 aacaaagtag tacgttacac gatcgcggca taaccggtaa ttccggtgcc agcctgcatg
     2461 ctaaaacctt tagcatcgat ggtaaaacgg tgtttatcgg ctctttcaat ttcgatccat
     2521 gttcgacatt gctcaatact gaaatgggct tcgtgataga gagcgaaacg ctggcacagt
     2581 taattgataa acgctttata cagagccagt atgatgcggc ctggcagctc cgtctggaca
     2641 ggtggggacg gatcaactgg gttgatcgtc atgcaaagaa agagattatt ctcaaaaaag
     2701 aacccgccac cagtttctgg aagcgggtta tggtcagact ggcgtcgata ttgcccgtgg
     2761 aatggttatt gtaaatgtgg cttaacgggc tggtgcttta tcgttctcac gcttaacaac
     2821 cggtttacca gaaaacaaaa acttcagtaa cgggatgcgt aaatgaattt catacagaat
     2881 tatcgcaatc cctactacga atatcaggcc acagagaaaa ccaagccagt tggaggtgat
     2941 gtgcggtgta atgtatgcgc cgaaaaacag cgttaacggg tggtgaacca gatagataaa
     3001 tagcgatgcg ttaacaaagt aagtcacccg cgctgactgg aagttaagca aacggtggcc
     3061 aaaggagaag accacattca ccatccacag accgaggacc atggtgatca ccgactcggt
     3121 ttcgtacatc caggcatcgc cactgccata gcgttggttg agtaaataag cgacaaatgc
     3181 caatgctgct gcaagggtac agccacgaga cggcgtggta aacaaggctt taagatgagg
     3241 gaaaatgaaa gccagtgcac cgaggataaa gaacggcaaa taaaatagcg tttgcatgac
     3301 aataaaattg aacatgccat tactcagaat gggcggatac acaataaaaa tcgttcttct
     3361 tattaccgca tagccgatgc cgaggcataa aaaaatcacc gatagtttta ccatcgagaa
     3421 ttttttattc gttttatcag aattttctaa attatttctg atgcgtttaa atatccatac
     3481 gcacagcgtc gtcatgacta ctaacaccag taaaaaccac aggtgtgata ttaattccca
     3541 ggccaacgta ttatatttgt catacaatga cagcccaggc caactttctg ctttcccttt
     3601 gacgtattgc agcataataa attgcggcaa tgtcagtaag gggatggctg ttaacatcgg
     3661 gatacctaca cgttcgacac gtactttcca ccattttttc aagggatagc gtaaaaaaag
     3721 catgtaggaa aagtagccgg atataacgaa aaataccagc atgcggaacg agtggatgaa
     3781 gtcattaaaa agggtcagcc acaatgacgg ttcggcgcta ttcacatgcc atgtatggct
     3841 cgaatagatt aaagaaatat gaaaagggat ccctaacaac atcagccagg cgcggatgga
     3901 gtcgaggaaa tattcacgtt gcgcgggtac tggattcata tatggttaac taatctcgga
     3961 tttttcgtct tatccctgtc gggttatgcc tttaggcttg ttgccatagc gacaccgacc
     4021 tgaccgcgcc aggcgcaggc ttcaaggttt ttatgcatag catcatcgct accactaacc
     4081 agaatggaag cgtctgtaag acggttgata aataaaattg ctggcaaacc ctacacgaag
     4141 tcgatgcttc tgtctttagg agaagcacgg aaagtgaaaa cggttgcaat cagttgctta
     4201 atccatgagc cagcgtgctg aacgataccg ggattctgtt gtcggaatgg ctggttatcc
     4261 attaaaatag atcggatcga tataagcaca caaaggggga agtgcttact aattatgaaa
     4321 cataaactac aaatgatgaa aatgcgttgg ttgagtgctg cagtaatgtt aaccctgtat
     4381 acatcttcaa gctgggcttt cagtattgat gatgtcgcaa agcaagctca atccttagcc
     4441 gggaaaggct atgaggcgcc caaaagcaac ttgccctccg ttttccgcga tatgaaatac
     4501 gcggactatc agcagatcca gtttaatcat gacaaagcgt actggaacaa tctgaagacc
     4561 ccattcaaac tcgagttcta ccatcagggt atgtacttcg ataccccggt caaaataaat
     4621 gaagtgactg ccaccgcagt caaacgaatc aaatacagcc cggattattt cactttcggc
     4681 gatgttcagc atgacaaaga cacggtaaaa gaccttggtt ttgccggttt taaagtgctt
     4741 tacccgatca acagcaaaga taaaaacgat gaaatcgtca gcatgctcgg ggccagctat
     4801 ttccgcgtga ttggtgcagg tcaggtttat ggcctttctg cccgcggcct ggcaattgat
     4861 accgccttgc catcgggtga agaatttcca cgcttcaaag agttctggat cgagcgtcca
     4921 aaaccgactg ataaacgttt aaccatttat gcattgcttg actcgccgcg cgcgacaggt
     4981 gcttacaaat tcgtggttat gccagggcgt gacacggttg tggatgtgca gtcgaaaatc
     5041 tatctgcgcg ataaagtcgg caaactgggg gttgcaccgt taaccagtat gttcctgttt
     5101 gggccgaacc aaccgtcgcc tgcaaataac tatcgtccgg agttgcacga ctctaacggt
     5161 ctctctatcc atgccggtaa tggcgaatgg atctggcgtc cgttgaataa cccgaaacat
     5221 ttagcggtta gcagcttctc gatggagaac ccgcaaggct tcggtctgtt gcagcgcggt
     5281 cgtgatttct cccgctttga agatctcgat gatcgttacg atcttcgtcc aagcgcatgg
     5341 gtgactccga aaggggagtg gggtaaaggc agcgttgagc tggtggaaat tccaaccaac
     5401 gatgaaacca acgataacat cgtcgcttac tggacgccgg atcagctgcc ggagccgggt
     5461 aaagagatga actttaaata caccatcacc ttcagccgtg atgaagacaa actgcatgcg
     5521 ccagataacg catgggtgca acaaacgcgt cgttcaacgg gggatgtgaa gcagtcgaac
     5581 ctgattcgcc agcctgacgg tactatcgcc tttgtggtcg attttaccgg cgcagagatg
     5641 aaaaaactgc cagaggatac cccggtcaca gcgcaaacca gcattggtga taatggtgag
     5701 atagttgaaa gcacggtgcg ctataacccg gttaccaaag gctggcgtct ggtgatgcgt
     5761 gtgaaagtga aagatgccaa gaaaaccact gaaatgcgtg ctgcgctggt gaatgccgat
     5821 cagacgttga gtgaaacctg gagctaccag ttacctgcca atgaataaga caactgagta
     5881 cattgacgca atgcccatcg ccgcaagcga gaaagcggca ttgccgaaga ctgatatccg
     5941 cgccgtacat caggcgctgg atgccgaaca ccgcacctgg gcgcgggagg atgattcccc
     6001 gcaaggctcg gtaaaggcgc gtctggaaca agcctggcca gattcacttg ctgatggaca
     6061 gttaattaaa gacgacgaag ggcgcgatca gctgaaggcg atgccagaag caaaacgctc
     6121 ctcgatgttt cccgacccgt ggcgtaccaa cccggtaggc cgtttctggg atcgcctgcg
     6181 tggacgcgat gtcacgccgc gctatctggc tcgtttgacc aaagaagagc aggagagcga
     6241 gcaaaagtgg cgtaccgtcg gtaccatccg ccgttacatt ctgttgatcc tgacgctcgc
     6301 gcaaactgtc gtcgcgacct ggtatatgaa gaccattctt ccttatcagg gttgggcgct
     6361 gattaatcct atggatatgg ttggtcagga tttgtgggtt tcctttatgc agcttctgcc
     6421 ttatatgctg caaaccggta tcctgatcct ctttgcggta ctgttctgtt gggtgtccgc
     6481 cggattctgg acggcgttaa tgggcttcct gcaactgctt attggtcgcg ataaatacag
     6541 tatatctgcg tcaacagttg gcgatgaacc attaaacccg gagcatcgca cggcgttgat
     6601 catgcctatc tgtaacgaag acgtgaaccg tgtttttgct ggcctgcgtg caacgtggga
     6661 atcagtaaaa gccaccggga atgccaaaca ctttgatgtc tacattctta gtgacagtta
     6721 taacccggat atctgcgtcg cagagcaaaa agcctggatg gagcttatcg ctgaagtcgg
     6781 tggcgaaggt cagattttct atcgccgccg ccgtcgccgc gtgaagcgta aaagcggtaa
     6841 tatcgatgac ttctgccgtc gctggggcag ccagtacagc tacatggtgg tgctggatgc
     6901 tgactcggta atgaccggtg attgtttgtg cgggctggtg cgcctgatgg aagccaaccc
     6961 gaacgccggg atcattcagt cgtcgccgaa agcgtccggt atggatacgc tgtatgcgcg
     7021 ctgtcagcag ttcgcgaccc gcgtgtatgg gccactgttt acagccggtt tgcacttctg
     7081 gcaacttggc gagtcgcact actggggaca taacgcgatt atccgcgtga aaccgtttat
     7141 cgagcactgc gcactggctc cgctgccggg cgaaggttcc tttgccggtt caatcctgtc
     7201 acatgacttt gtagaagcgg cgttgatgcg ccgtgcaggt tggggggtct ggattgctta
     7261 cgatctcccg ggttcttatg aagaattacc gcctaacttg cttgatgagc taaaacgtga
     7321 ccgccgctgg tgccacggta acctgatgaa cttccgtctg ttcctggtga agggtatgca
     7381 cccggttcac cgtgcggtgt tcctgacggg cgtgatgtct tatctctccg ctccgctgtg
     7441 gtttatgttc ctcgcgctct ctactgcatt gcaggtagta catgcgttga ccgaaccgca
     7501 atacttcctg caaccacggc agttgttccc ggtgtggccg cagtggcgtc ctgagctggc
     7561 gattgcactt tttgcttcga ctatggtgct gttgttcctg ccgaagttat tgagcatttt
     7621 gcttatctgg tgcaaaggaa cgaaagaata cggcggcttc tggcgcgtta cattatcgtt
     7681 gctactggaa gtgctttttt ccgtgctgct ggctccggta cgcatgctgt tccatacggt
     7741 cttcgttgtc agcgcgttcc ttggctggga agtggtgtgg aattcaccgc agcgtgatga
     7801 tgactccact tcctggggtg aagcgttcaa acgccacggc tcacagctgc tgttagggtt
     7861 agtgtgggct gttgggatgg cgtggctgga tctgcgtttc ctgttctggc tggcaccgat
     7921 tgtcttctcg ttgatcctgt caccgtttgt ttcggtgatt tccagccgtg ccaccgttgg
     7981 tctgcgaacc aaacgctgga aactgttcct gatcccggaa gagtattcac cgccgcaggt
     8041 gctggttgat accgatcgct tccttgagat gaatcgtcaa cgctcccttg atgatggttt
     8101 tatgcacgcg gtgtttaacc cgtcatttaa cgctctggca accgcaatgg cgaccgcgcg
     8161 tcaccgcgcc agtaaggtgc tggaaatcgc ccgtgaccgc cacgttgaac aggcgcttaa
     8221 cgagacgcca gagaagctga atcgcgatcg tcgcctggtg ctgctaagcg atccggtgac
     8281 gatggcccgt ctgcatttcc gtgtctggaa ttccccggag agatattctt catgggtgag
     8341 ttattacgaa gggataaagc tcaatccact ggcattgcgt aaaccggatg cggcttcgca
     8401 ataaaaacgt agttgcctga tgcgctacgc ttatcaggcc tacatcgttc ctgcaattta
     8461 ttgattttgc aagattttgt aggttgggta aggcgttcac gccgcatccg gcaaaaacaa
     8521 cgagccaata aaaataccgg cgttatgc
//
LOCUS       DAJTMK010000159         8520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTMK010000159 DAJTMK010000000
VERSION     DAJTMK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.163"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..208
                     /locus_tag="OO135_003301"
     CDS             <1..208
                     /locus_tag="OO135_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5399041.1"
                     /translation="QTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRD
                     GESSPAEYSVSLTRADMIEFTMEH"
     gene            209..661
                     /gene="phnG"
                     /locus_tag="OO135_003302"
     CDS             209..661
                     /gene="phnG"
                     /locus_tag="OO135_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5399042.1"
                     /translation="MHADIATRQHWMSVLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            658..1242
                     /gene="phnH"
                     /locus_tag="OO135_003303"
     CDS             658..1242
                     /gene="phnH"
                     /locus_tag="OO135_003303"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5399043.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            1242..2306
                     /gene="phnI"
                     /locus_tag="OO135_003304"
     CDS             1242..2306
                     /gene="phnI"
                     /locus_tag="OO135_003304"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5399044.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            2299..3144
                     /gene="phnJ"
                     /locus_tag="OO135_003305"
     CDS             2299..3144
                     /gene="phnJ"
                     /locus_tag="OO135_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5399045.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            3141..3899
                     /gene="phnK"
                     /locus_tag="OO135_003306"
     CDS             3141..3899
                     /gene="phnK"
                     /locus_tag="OO135_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5399046.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSFLQN"
     gene            4010..4690
                     /gene="phnL"
                     /locus_tag="OO135_003307"
     CDS             4010..4690
                     /gene="phnL"
                     /locus_tag="OO135_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5399047.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            4687..5823
                     /gene="phnM"
                     /locus_tag="OO135_003308"
     CDS             4687..5823
                     /gene="phnM"
                     /locus_tag="OO135_003308"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5399048.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQVALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            5823..6380
                     /gene="phnN"
                     /locus_tag="OO135_003309"
     CDS             5823..6380
                     /gene="phnN"
                     /locus_tag="OO135_003309"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5399049.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            6367..6801
                     /gene="phnO"
                     /locus_tag="OO135_003310"
     CDS             6367..6801
                     /gene="phnO"
                     /locus_tag="OO135_003310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5399050.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            6803..7561
                     /gene="phnP"
                     /locus_tag="OO135_003311"
     CDS             6803..7561
                     /gene="phnP"
                     /locus_tag="OO135_003311"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5399051.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(7708..8037)
                     /gene="yjdP"
                     /locus_tag="OO135_003312"
     CDS             complement(7708..8037)
                     /gene="yjdP"
                     /locus_tag="OO135_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR5399052.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(8106..8423)
                     /locus_tag="OO135_003313"
     CDS             complement(8106..8423)
                     /locus_tag="OO135_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR5399053.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         1807 a         2593 c         2475 g         1645 t
ORIGIN      
        1 gcaaaccggg attgcgctgc gccgcagcca gacgcggatc agcgcccgcc gcgcccaggc
       61 caaagagtgc cagcgtcttg aaatcccgaa tatgtcgccg ctgctgtgcg tgcgcaccct
      121 taaccaccgt gacggtgaaa gcagcccggc ggagtactcc gtcagcctga cgcgcgccga
      181 catgattgaa ttcactatgg agcactgaat gcacgcagat atcgcgaccc gccagcactg
      241 gatgtccgtg ctggcgcaca gccaaccggc tgaactggca gcccgcctga acgcgctgaa
      301 catcaccgcc aactatgaag tgatccgcac cgccgaaacc ggcctggtac agattcaggc
      361 gcgaatgggc ggtaccggcg aacgcttttt tgccggcgac gccacgctga cccgcgccgc
      421 cgtacgcctg actgacggca ccctcggcta cagctgggtg ctggggcgcg acaaacagca
      481 cgccgaacgc tgcgcgctga ttgacgcgtt gatgcagcaa tctcgccatt ttcaaaactt
      541 atcagaaacc cttattgccc cactggacgc tgaccgtatg gcacgcattg ccgcacgcca
      601 ggccgaagtg aacgccagcc gggtcgactt ctttacgatg gttcgcggag acaacgcatg
      661 accctggaaa ccgcttttat gcttcctgtg caggatgccc agcacagttt tcgtcgcctg
      721 ttaaaggcca tgagcgagcc gggcgtgatt gtcgccctgc atcagctcaa acgcggctgg
      781 cagccgctga atatcgccac caccagcgtg ctgctgacgc tggctgataa cgacacgccg
      841 gtgtggcttt ccgcgccatt aagtaacgat atcgtcagcc agagcctgcg ttttcatacc
      901 aacgcgccgc tggtcaatca gccggaactg gcgaccttcg cggtaacgga tgaggcgatt
      961 tccagcgaac agctcaacgc tctttccagc ggcaccgctg ttgcgccgga agcgggcgcg
     1021 acgctgattt tacaggtcgt cagcctgagc ggcgggcgca tgctgcgcct tactggcgcg
     1081 ggtattgccg aagaacgaat gatcgctccg cagctgccgg agtgcattct gcacgaactc
     1141 accgagcgcc cgcacccgtt cccgctcggc atcgacctga tcctgacctg cggcgagcgg
     1201 ctgctggcta tcccgcgaac cactcatgtg gaggtgtgct gatgtacgtt gccgtgaaag
     1261 ggggcgagaa ggagatcgac gccgctcacg ccctgcaaga gagccgacgc cggggcaata
     1321 ccgatttgcc tgaactgagc gtcgcccaga ttgaacagca gcttaatctt gcagtagatc
     1381 gcgtgatgac cgaaggcggc attgccgacc gcgaactggc ggcgctggcg ctgaaacagg
     1441 ccagcggcga taacgtcgaa gcgattttcc tgctacgcgc ctaccgcacc acgctggcga
     1501 agctggcggt aagcgagccg ctcgacacca cggagatgcg tctcgaacgg cgtatttcag
     1561 cggtttataa agatattccc ggcggtcagt tgcttggccc aacctacgac tacacccatc
     1621 gcctgctcga tttcaccctg ctggcaaacg gcgaagcgcc gacgctgacc accgcagaca
     1681 gcgaacaatc cccgtcgccg cacgttttca gcctgctggc gcgtcagggg ctggcgaagt
     1741 ttgaagagga tagcggcgca cagccggatg acatcacccg cacgccgcca gtttacccct
     1801 gttcacgttc ttcccgtttg cagcagttga tgcgcggcga cgaaggctat ttgctggcgc
     1861 tggcctactc tacccagcgc ggttacggac gcaatcaccc gttcgcgggc gaaatccgca
     1921 gtggttacat cgacgtgtcg attatgccgg aagagctggg atttgcggtg aacgtcggcg
     1981 aactgctgat gaccgagtgt gaaatggtca acggttttat tgacccgccg gatgagccgc
     2041 cgcacttcac gcgcggctac gggctggtgt tcggcatgag cgagcgcaaa gcgatggcga
     2101 tggcgctggt tgaccgtgcg ttgcaggctc cggagtacgg cgagcacgcg acaggcccgg
     2161 cgcaggatga agagttcgtg ctggcacatg ccgacaacgt cgaagccgca ggctttgtct
     2221 cgcacctcaa actcccccac tacgtcgatt tccaggccga actggagcta ctcaaacgtc
     2281 tgcaacagga gcagaaccat ggctaatctg agcggctaca actttgccta cctcgacgag
     2341 cagaccaaac gcatgatccg ccgcgccatc ttaaaagcgg tggcgatccc cggttatcag
     2401 gtgccgtttg gcgggcgcga gatgccgatg ccctacggct ggggaaccgg cggcattcag
     2461 ctcaccgcca gcgtgattgg cgaaagcgac gtgctgaagg tgattgacca gggcgcagac
     2521 gacaccacca acgccgtgtc gattcgcaac ttctttaagc gcgtgaccgg ggtaaacacc
     2581 actgaacgta cggacgatgc gacgcttatc cagacgcgcc atcgcatccc cgaaacgccg
     2641 ctgaccgaag atcagatcat tatcttccaa gtgccgatcc ccgaaccgct gcgctttatc
     2701 gagccgcgcg aaacggaaac ccgcaccatg cacgcgctgg aagagtacgg cgtgatgcag
     2761 gtgaaactgt atgaagatat cgcccgcttc ggtcatatcg ccactaccta cgcctatccg
     2821 gtgaaggtga acgggcgcta cgtgatggac ccgtcgccga tcccgaaatt cgataaccca
     2881 aaaatggaca tgatgcccgc cctgcaactg ttcggcgcgg ggcgcgagaa gcgcatctat
     2941 gcggtgccgc cgtttacccg cgtggaaagt ctcgatttcg acgatcaccc gttcaccgtt
     3001 cagcagtggg atgagccatg cgccatctgc ggatcgaccc acagctatct tgatgaagtg
     3061 gtgctggatg acgccggaaa ccgcatgttt gtctgctccg ataccgatta ttgccgccaa
     3121 cagagcgagg caaaaaacca atgaatcaac cgttactttc ggtcaataac ctgacccacc
     3181 tttacgcgcc gggcaaaggc tttagcgatg tctcttttga tttatggccg ggggaagtgc
     3241 tgggcattgt cggggagtcc ggctccggaa agaccacgct gctgaaatca atctccgcgc
     3301 gcctgacgcc gcagcagggg gagattcact atgagagccg ttcgctgtac gcaatgagcg
     3361 aggccgaccg ccgtcgcctg ctgcgcaccg aatggggcgt agtgcatcag catccgctcg
     3421 acggcctgcg ccgccaggtg tcggcaggcg gcaatatcgg cgagcggctg atggcgaccg
     3481 gggcacgtca ttacggcgat attcgtgcca ccgcgcagaa gtggctggaa gaggtggaaa
     3541 ttcccgccaa ccggatcgac gacctgccga ccaccttttc cggcggcatg cagcaacgtt
     3601 tgcagatcgc ccgcaacctg gtgacgcatc cgaagctggt gtttatggac gagccgaccg
     3661 gcggactgga tgtgtcggta caggcacgcc tgctcgacct gctgcgcggc ctggtggtgg
     3721 agctgaacct cgcggtagtg attgtcaccc acgatttagg cgtcgcccgc ctgctggcgg
     3781 accgtttgct ggtgatgaag caggggcaag tggtggagag tgggttaacc gaccgcgtgc
     3841 tcgacgaccc gcaccatccg tatacacaac tgctggtgtc atcgtttttg cagaattgag
     3901 ccggtgccgg atgcggcgta aacgccttat ccggcctaca aatgcgctcc ccgtaggtcg
     3961 gataagacgc gtcagcgtcg catccgacac ccgaaccacg aggcgaaaaa tgattaacgt
     4021 acaaaacgtc agtaaaacct tcatcctgca tcagcaaaac ggcgtacgcc tgcccgtctt
     4081 acaacgtgcc tcgctcaccg tcaacgcagg cgaatgcgtg gtgctccacg gtcactccgg
     4141 cagcggcaaa tcaaccctgc tgcgctcgct gtacgccaac tatctacccg acgaaggtca
     4201 aatccagatc aaacacggtg acgagtgggt agacctggtc accgcgccag cgcgcaaagt
     4261 ggtggaaatc cgcaaaacca cagtcggctg ggtgagccag tttctgcgcg tcatcccgcg
     4321 tatctcagca ctggaagtgg tgatgcagcc gctgctcgat accggcgttc cgcgtgaagc
     4381 ctgcgccgct aaagccgcgc gtcttctcac ccgcctgaac gtgccggaac gcctgtggca
     4441 cctggcacca tcgaccttct ccggcggcga acagcagcgc gtcaacatcg cccgcggctt
     4501 tatcgtcgac taccccattc tgctgctcga cgaacctacc gcctcgctgg acgccaaaaa
     4561 cagcgccgcg gtggtggaac tgattcacga agccaaagcc cgtggcgcag ccatcgtcgg
     4621 catcttccat gacgaagctg tacgtaatga cgtcgccgac cgcctgcacc caatgggagc
     4681 ctcttcatga ttatcaataa cgttaagctg gtgctggaaa acgaggtggt gcacggttcg
     4741 ctggaggtgc aggatggcga aatccgcgcc tttgccgaaa gccagagccg cctgccagaa
     4801 gcgatggacg gcgaaggtgg ctggctacta cccggcctga ttgagctgca taccgacaac
     4861 ctcgataaat tcttcacccc gcgcccgaaa gtcgactggc ccgcccattc ggcgatgagc
     4921 agccacgacg cgctgatggt ggcaagcggc atcaccaccg tgctggacgc ggtggcgatt
     4981 ggcgacgtgc gcgacggcgg cgatcggctg gagaatctgg agaagatgat caacaccatc
     5041 gaagagacgc agaaacgcgg cgtcaaccgc gccgagcacc gtctgcacct gcgctgcgag
     5101 ctgccgcatc acaccacact gccgctgttt gaaaaactgg ttcagcgcga accggtgacg
     5161 ctggtgtcgc tgatggacca ctcaccgggc cagcgccagt tcgctaaccg cgagaagtat
     5221 cgcgaatatt atcagggcaa atactccctc accgatgcgc agatgcagca gtacgaagaa
     5281 gagcaattgg cgctcgccgc acgctggtcg cagccgaatc gcgaatccat cgccgccctg
     5341 tgccgcgcgc gacaagttgc gctcgccagc cacgatgacg ccacgcacgc ccacgttgcc
     5401 gaatcccacc aacttggcag cgtgatcgcc gaatttacca ccacgttcga agcggcggaa
     5461 gcctcgcgta agcatggcat gaacgtgctg atgggtgcgc caaatatcgt gcgtggcggc
     5521 tcgcactccg gcaatgtggc ggccagtgaa ctggcgcagc ttggtctgct ggatatcctc
     5581 tcttccgact actaccccgc cagcctgctg gatgcggcgt tccgcgtcgc cgatgacgag
     5641 agcaaccgct ttacgctacc gcaggcggtg aggctggtga ccaaaaatcc ggcgcaggcg
     5701 ctgaatcttc aggatcgcgg ggtgattggc gagggtaaac gcgctgacct ggtgctggcg
     5761 catcgccagg gcaatcacat tcatatcgac cacgtctggc gtcagggtaa aagggtgttc
     5821 tgatgatggg aaaactgatt tggttaatgg ggccgtccgg ctccgggaaa gacagcctgc
     5881 tggcggaact ccgcctgcgg gaacaaacgc agttactggt ggcgcatcgc tacatcacgc
     5941 gcgacgccag cgccggaagt gaaaaccata tcgccctgag tgagcgggag ttttttaccc
     6001 gcgcggggca aaacctgctg gccttaagct ggcacgctaa cggtctgtat tatggcgtcg
     6061 gcatcgagat tgacctctgg ctgcacgctg gattcgacgt agtcgtcaac ggttcacgcg
     6121 cgcatttgcc gcaggcacgg gcgcgctatc aatcggcgct gctgcccgtc tgtttacagg
     6181 tttcgccgga gatcctgcgc cagcgcctga aaaaccgtgg ccgtgaaaac accagtgaaa
     6241 ttaacgcccg gctggcgcgc gccgcccgct atactccaca ggattgccat acgctcaaca
     6301 atgacggcag cctgcgccag tcggtcgaca cactgctgac gctgattcat cagaaggaga
     6361 aacaccatgc ctgcttgtga gcttcgcccg gccacgcagt acgataccga cgcggtttac
     6421 gcgctgattt gtgagctaaa acaggcggag tttgaccacc acgcgtttcg cgtgggcttt
     6481 aacgccaacc tgcgcgaccc gagcatgcgc taccatctgg cgctgctgga tggcgaagtt
     6541 gtcggcatga tcggcctgca tttgcagttt catctgcatc atgtcaactg gatcggcgaa
     6601 attcaggaac tggtggtgat gccgcaggcg tgcggtctga acgtcggcag taagttactg
     6661 gcgtgggcag aagaagaagc ccgccaggcc ggggccgaaa tgaccgaact ttccaccaac
     6721 gtgaagcgcc acgacgcgca ccgtttttac ctgcgtgaag gctacgagca gagccacttc
     6781 cgcttcacca aggcgctgta acatgagcct gaccctcacg ctcaccggca ccggcggcgc
     6841 acagggcgtt ccggcatggg gctgcgagtg cgtagcctgc gccagagcgc ggcgctcacc
     6901 gcagtatcgc cgccagccat gcagcggcgt agtgaagttt aacgacgcaa tcaccctgat
     6961 cgacgccggg ctgcacgatc tcgccgatcg ctggtcgccc ggatcgttcc agcagttttt
     7021 gctgacgcac taccatatgg atcacgtgca ggggctattc ccgctgcgct ggggcgtggg
     7081 cgatccgatc ccggtttacg gcccgccgga tgaacagggc tgcgacgatc tgtttaaaca
     7141 tccgggcctg cttgatttca gccacacggt ggaaccgttt gtggtgtttg atttgcaggg
     7201 gttacaggtc acgcccctgc cgctcaacca ctcaaaactg accttcggtt atctgctgga
     7261 aacggcacac agccgggtgg cgtggctgtc tgacaccgca ggcttgccgg aaaaaacgct
     7321 gaaattttta cgcaataatc agccgcaggt aatggtgatt gattgcagcc acccgccgcg
     7381 cgaggatgcg ccacgtagtc actgtgattt aaacaccgtg ctggcgctga atcaggttat
     7441 ccgctcgcca cgggtgattc tgacccatat cagccaccag tttgatgcgt ggctgatgga
     7501 aaacgcactg ccgtcggggt ttgaggcggg gtttgatggg atggagattg gggtggcgtg
     7561 atgagaggga atgtgcgcgc tggccccctc accctaaccc tctccccaga ggggcgaggg
     7621 gaccgattgt actcgatatt gaatattgcg ctggttttct ccctctcccc attggggtga
     7681 ggggcgatgc ctgctccata cccaacctca tcgcccatac tcatcttcca ttctccgctc
     7741 ttcatcttct agctgccgac gctcctgatc aagctggcgc tggcgatcgt ccagctgcct
     7801 gcggcgatct tcaaactggc ggcggcggtc gtcatattgt ctgcgccgat cgtcgctcac
     7861 ttcacgctgc cagccgtcgt cgcgcgaatc ttcatagtct cgcccacggt cagggttata
     7921 agcgtcgtta atcgcctgct gaatattgcc aatggtgtcg tcgataatat cggcctgggc
     7981 cggaacggtg gacagcgtga gcagggtgaa taaaagaaat agcggaaagc gtttcattag
     8041 ccaacctcaa aaagaaactc tatccacatt aatcattact catccatgca agtagtggat
     8101 gaatctcaat ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg ttgatgacga
     8161 ccgccttcgt actgtgcgcc cagccacgca tccacaatca tttttgccag ttcgaggcca
     8221 accactcgtg aaccaaaagc cagtgcaact tcactggcat aatgcggata atcagtgcgc
     8281 tctgacgacc tggtgtcttt atcaatcacc tcactcattg accatgaata aaaggctggc
     8341 acagcaacgc agaatcctgc ggtgccagcg acatgaccgc ccattgctcc ggtacgtttt
     8401 ttaaaaagaa acaccggttg cattgataaa ttgtgctcag tatttgccga agtacaatat
     8461 atataataaa tatataaaac acctcatatt atttacagag ataataaatt cgcgtaattt
//
LOCUS       DAJTMK010000160         8410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTMK010000160 DAJTMK010000000
VERSION     DAJTMK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.164"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..971)
                     /locus_tag="OO135_003314"
     CDS             complement(<1..971)
                     /locus_tag="OO135_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5399054.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSAEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            1063..1149
                     /locus_tag="OO135_003315"
                     /pseudo
     CDS             1063..1149
                     /locus_tag="OO135_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1450..1539
                     /locus_tag="OO135_003316"
                     /pseudo
     CDS             1450..1539
                     /locus_tag="OO135_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1563..6077
                     /locus_tag="OO135_003317"
                     /pseudo
     CDS             1563..6077
                     /locus_tag="OO135_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            6078..7727
                     /locus_tag="OO135_003318"
     CDS             6078..7727
                     /locus_tag="OO135_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5399055.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7732..>8410
                     /locus_tag="OO135_003319"
     CDS             7732..>8410
                     /locus_tag="OO135_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399056.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYMKWSTKTGHRV
                     RVFPVSIF"
BASE COUNT         2050 a         2014 c         2358 g         1988 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatccgtgg gccgtggtag tccggcgaag aggttaatct ttgcagggct
      181 tgcagagtgc gagtcgacaa tggattgtta taaatattaa catctgcata aatgtgcaga
      241 ttcatgatac tgtctggcaa tgtgttcagc tgattgttag ggatatatag tgattgcaga
      301 cgttgtggaa gttcgggcag tctgtgtagt tggttttccc caacgtttaa aagcctcaga
      361 ttttgaggaa ggggcggtag ctcttgtaat ctgttatcaa aagcacttag ttcctgtagt
      421 tcacatggta attctggtaa ggcagtaagc tggttgttgc caacagaaag tttttgtaga
      481 gccgggggaa gatcaggtag cgtctccagg ctattgagaa gggctgagag tgactgcaag
      541 gaagatggaa gagaaggtaa acatgttaaa actctgttaa gtgaaacatc aagagcgacc
      601 aggtgaggcg gaagagccgg gagcctgctc agtctgttat cgctggcttt aagtacagtt
      661 aaggacggag ggagttctgg cagagagcgt agctcattgc tagagatgtt aagttcttgt
      721 atgtgcgggg gcaggtatgg gagagagcgt aatctgagta aacttaaatt gagggctggc
      781 tcttgaaaag ccagacatat tttcagtaat cgaactgcct gtgttcggtc ttctgttgca
      841 gcaccttcct tggcccagtt atcccagatg cggtgataat ccgtgatatt ttgctcttct
      901 gcggataaac gggatataca tcctaaacca caaaaggagc ggaaaaaaga aggattgtgg
      961 gcaggtttca tttattattg atcctcattg tttatatatt aatatgttcg tgcaaatgga
     1021 gtgaggattc tttaaaaaaa cgctcctgta aatagttaag aacaggaaga actgttgggg
     1081 atgtggctgg ccgaaaatga aggggtgaag ttctggctca atgtgctgac tgaactgaaa
     1141 aaacgcggtt ggtcgcccta ccttttcatc aagccagtag tctgcccacc actgcatcat
     1201 ttctctgcgc ttatcgagat actgagcatg gttgtaaatt caactaaccc agtgcgttga
     1261 caaaaaatta gcgcaagaag acaaaaaatc accttgcgct aatgctctgt atcaggtcac
     1321 taataccagc taagtagttg attcatagtg actgcatatg ttgtgctttg tcgcattttg
     1381 tagtctatta tttaatctgc aacaacgata atacaatgaa ttttaattgg tttttgtgat
     1441 tttgtccttc ttctgacatg gaaggacacc cgatggatac ccaacgattc caatcccaat
     1501 ttcattggca tttatcgttt aaattttctg gcgcgatagc agcgtgcttg tctctgtctc
     1561 ttgtgggaac agggcttgct aatgctgatg attcgcttcc ttccagtaac tatgcgccgc
     1621 ccgccggggg aacattcttt ttgcttgctg acagcagttt tagcagcagt gaagaggcga
     1681 aagtgcgact ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa gagtacggcg
     1741 gcgtggacgt tcgcctgtat cgtattcctg acccgatggc atttttgcgc cagcagaaaa
     1801 acctgcatcg cattgtggtg caaccgcaat atctgggcga cgggctgaac aatacgctga
     1861 cctggctgtg ggataactgg tacggcaaat ctcgccgcgt aatgcagcgt actttctctt
     1921 ctcagtcacg gcagaatgtg actcaggcat tacccgaatt acatctcggc aatgccatta
     1981 ttaaaccttc ccgttatgta cagaacaacc agttttcccc gctgaaaaaa tatcccttgg
     2041 tggaacagtt ccgttatcca ctatggcagg ctaaaccggt cgagccgcag caaggggtaa
     2101 aactggaagg cgcatccagc aatttcatct cgccgcagcc gggtaacatt tatattcctc
     2161 tcggccaaca agagccggga ctgtacctcg tcgaggcgat ggttggtggg tatcgggcga
     2221 cgacggtggt gtttgtttcc gataccgtgg ctcttagcaa agtgtcaggc aacgagcttc
     2281 tggtgtggac cgcgggtaaa aaacagggtg aagcgaagcc cggctcagag atcttgtgga
     2341 ctgacggtct tggcgtgatg acccgcggtg tgaccgatga cagcggtacc ttgcagttac
     2401 aacatatatc gccagaacgt tcatacattc tgggtaagga tgctgaaggc ggcgtttttg
     2461 tctccgagaa cttcttctac gaaagcgaaa tctacaacac ccgcttgtat atttttaccg
     2521 atcgcccgct atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc cgcgagttcc
     2581 acgatccgtt gcattcatcc cccatcgtca gcgccccggc gaagctttcg gtgctggacg
     2641 ctaacggcag tctgttgcaa accgtcgatg tcacgctgga tgcgcgcaat ggcgggcagg
     2701 gaagtttccg cctgccagaa aatgccgttg ccggaggtta tgagttacgt cttgcttacc
     2761 gcaatcaggt ctatagcagc agttttcgcg tggcaaacta catcaagcca catttcgaga
     2821 ttggtttagc tctcgacaaa aaagagttca aaactggcga agcggtcagc ggcaaactgc
     2881 aactgctcta cccggatggc gagccggtaa aaaatgcccg cgtgcagtta agtttgcgcg
     2941 ctcagcaatt atcaatggtc ggtaacgatt tgcgttatgc cggacgtttc cccgtgtcgc
     3001 tggaaggcag cgaaacggtg tccgacgcca gcggtcatgt ggcgttaaat ctccccgccg
     3061 ccgataaacc gagccgttat ttgttaaccg tctccgccag tgacggcgcg gcgtatcgtg
     3121 tcaccaccac caaagagatc ctcattgaac gcggtctggc gcattactca ttaagtactg
     3181 ccgcacaata cagtaatagc ggcgagtcgg ttgtgttccg ttatgccgcg ctggaatctt
     3241 caaaacaggt tcctgttacg tatgaatggt tgcgtctcga agaccgcacg agccatagcg
     3301 gagagctacc gtcaggcggc aaatccttta ccgtcaattt cgctaaacct ggcaactaca
     3361 atctgacatt acgcgataaa gacggcttaa ttctcgctgg gttaagttat gccgtcagcg
     3421 gtaagggcag cacggcgcat acgggtacgg tagatatcgt ggcggataaa acgctgtacc
     3481 agccaggcga aaccgcgaag atgctgatta cctttccgga gccaattgat gaagcattat
     3541 tgacgctgga acgcgatcgc gtggaacagc agtcgctgct ttcgcatccg gcaaactggc
     3601 taacgctaca acgtttaaac gatacccagt atgaagcccg ggttccagtg agcaattcct
     3661 ttgcgcctaa catcactttt tcggtgctgt atacccgtaa tggtcagtac agttttcaga
     3721 acgccgggat caaagttgcc gttcctcagc ttgatatccg ggtgaaaacg gacaaaaccc
     3781 attaccagcc tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa ggtaaacctg
     3841 tttctgcaca gctaacggta ggcgtggtcg atgaaatgat ctacgcgctg caaccagaaa
     3901 tcgcgccgaa tatcggcaaa tttttctatc cgctggggcg taacaatgtg cgtaccagct
     3961 ccagtctgtc gtttatcagc tacgaccagg cgctctccag cgagccggtt gcgcctggcg
     4021 caactaaccg cagcgagcgg cgagtaaaaa tgcttgaacg tccacggcgt gaagaggtgg
     4081 ataccgcggc atggatgccg tcactcacaa ccgataaaca aggcaaagcg tatttcacgt
     4141 tcctgatgcc tgattcgtta acccgctggc gtatcaccgc gcgtgggatg aacggcgacg
     4201 ggctggtcgg gcaggggcgt gcttatctgc gttcggaaaa aaatctctac atgaagtgga
     4261 gtatgccaat ggtgtatcgc gtgggcgaca aaccggcggc aggactgttt atcttcagtc
     4321 agcaggataa cgaaccggtg gcgctggtga ctaaatttgc aggcgctgag atgcgccaga
     4381 cgctgacgct gcacaaaggg gcgaattata tttcgctggc gcagaatatt cagcaatctg
     4441 gcttgttaag tgcagaactg caacaaaatg ggcaagtgca ggacagcatt agcacaaaac
     4501 tgtcttttgt ggacaacagc aggcccgttg aacagcagaa aaatgtcatg ctcggcggtg
     4561 gcgagaacgc gctgatgttg cccgagcagg cgagtaatat ccggctacaa agtagtgaaa
     4621 cgccgcagga gattttccgc aacaatcttg atgcgttagt cgatgaaccg tggggtgggg
     4681 tgatcaacac cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg cttgccgctc
     4741 atcaaagtgc cgccgctaac gacattcgtc agatgattca ggataaccgt ctgcggctga
     4801 tgcaactggc ggggcccgga gcgcgcttta cctggtgggg tgaagatggc aatggtgacg
     4861 ccttccttac ggcatgggca tggtacgccg actggcaggc cagctaggcg ctcggcgtaa
     4921 cgcaacaacc ggaatactgg cagcatatgc tcgacagtta tgccgagcag gcggataaca
     4981 tgccgttatt gcatcgggcg ctggtgctgg cgtgggcaca ggagatgaat ctgccgtgca
     5041 aaacgttgtt gaaagggttg gatgaagcta tcgcccggcg cggaactaaa gatgaagatt
     5101 tctctgagga agacatccgc gatatcaatg acagcctgat cctcgataca ccggaatctc
     5161 cactggcaga tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga
     5221 agtccacggt gatgccacag gttcagcaat atgcgtggga taaagcggca aacagcaatc
     5281 agccgctggc gcacacggtt gtgctgctca atagcggtgg tgacgctacc cagacggccg
     5341 ctattttaag tggtttgacc gctgagcaat ccactattga gcgcgcgctg gccatgaact
     5401 ggctggcgaa atatatggcg acaatgcctc cagttgtttt gcctgcgcct gcgggcgcat
     5461 gggctaaaca taagttaact ggagggggcg aatactggcg ttgggttggt cagggcgtgc
     5521 cggacaatct ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc cgctggcgtg
     5581 agccggcaaa aatggctcaa caaagtaaca ttccggtgac cgttgaacgc cagttgtatc
     5641 ggcttatccc tggtgaagaa gagatgagct ttattctgca accggtgacc agcaatgaga
     5701 ttgacagcga tgcgctgtat ctcgatgaaa tcacgcttac cagcgagcag gatgcagttc
     5761 tgcgctacgg tcaggtggaa gtaccgctgc caccgggagc cgacgttgag cgcacaacat
     5821 ggggcatttc ggtcaataaa cccaacgccg cgaaacagca ggggcaattg ctggaaaaag
     5881 cgcgaaatga aatgggcgaa ctggcctata tggtgccggt gaaagaactg acgggaacgg
     5941 tcactttccg ccatttgctg cgcttctcgc aaaaagggca attcgttctg cctcctgctc
     6001 gttatgtgcg ttcctatgca cctgcacagc aaagtgttgc ggcagggagt gaatggaccg
     6061 ggatgcaggt gaaataagtg aactggcgca gaatcgtctg gctattggcg ttggttactc
     6121 agccaacgct tgcggaggag acgccgttac aactggcact gcgtggtgcg cagcacgatc
     6181 aactgtatca gctgtcgtca tcaggcgtga ccacagtctc agcattacct gactcgctca
     6241 ccacgccgtt agggagtttg tggaaactgt atgtttacgc ctggctggag gatacccatc
     6301 aaccggagca gccctatcag tgtcgcggaa actccccgga agaggtttat tgttgtcagg
     6361 ctggggagag cataacgcgc gataccgcgc tggtacgttc ctgtgggctc tattttgcgc
     6421 cgcagcggtt gcatattggc gcagatgtgt gggggcaata ctggcaacag cgtcaggcgc
     6481 ctgcgtggtt agcgtctctt actacgctga agccagaaac ctcggtaacg gttaaatcgc
     6541 tgcttgattc attagcgacg ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg
     6601 tggtgctgga cgaggcgaaa atcggtgtcg catccatgct cggtagtcgg gtacgggtga
     6661 aaacctggag ctggtttgcc gatgataaac aggaaatccg tcagggtggt tttgccggct
     6721 ggttaaccga tggtacaccg ctgtgggtta ctggcagtgg caccagcaaa acggtactaa
     6781 cccgttatgc gacggtgctt aatcgggttt tgccagtgcc gacgcaggtt gccagtgggc
     6841 aatgtgtaga ggtggagctg tttgcccgct atccgctgaa gaaaatcaca gcggaaaaga
     6901 gtacgacggc agtaaaaccc ggcgtactta atgggcgcta tcgtgtgacg tttactaatg
     6961 gtaaccacat tacgtttgtc agccacggtg agacgacctt actgagcgaa aagggcaaac
     7021 tgaaattgca gtcgcatctc gatcgtgaag agtacgttgc cagagttctg gatcgcgagg
     7081 cgaaaagtac gccccctgag gcagcgaaag ccatgaccgt ggcgattcgc acttttttac
     7141 agcaaaacgc caatcgggaa ggcgattgcc tgaccatccc cgacagcagc gccacgcagc
     7201 gtgtttctgc ttcgccagcg accacggggg cgcgaacgat gaccgcctgg acgcaggatc
     7261 tcatctatgc aggcgatccg gttcattatc atggcagtcg agctaccgaa gggacacttt
     7321 cctggcggca agccacggcc caggcggggc agggagagcg ttacgaccag atactcgcgt
     7381 ttgcttatcc cgacaacagc cttagccgct ggggagcgcc acgctcaacc tgccagttat
     7441 tacccaaagc gaaagcctgg ctggcgaaaa aaatgccgca gtggcggcgt atattacaag
     7501 gcgagacggg gtacaacgaa ccagacgtgt ttgcggtctg tcgtttagtc tcaggtttcc
     7561 cctataccga tcggcagcag aaacggctgt ttattcgtaa cttctttacg cttcaggatc
     7621 ggctggattt aacccatgag tatctgcacc tggcctttga tggttatccc accgggcttg
     7681 atgagaacta tatcgaaacg ctgacccgac aattattaat ggactaatgc tatgcgaaag
     7741 atttttcttc cgttgttact ggtggcactt tcgcctgtcg ctcacagtga aggtgtgcag
     7801 gaagtcgaga ttgacgcacc gctctctggc tggcatccgg cagagggcga agatgccagt
     7861 ttttcacaat cgatcaatta cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc
     7921 tcagcgcaga tccgcggcaa aataaagaat tatgctgcgg cgggtaaaat tcagcagggc
     7981 cggctggtgg tcaacggtgc cagtatgccg cagagagttg aatccgatgg ttcatttgca
     8041 cgcccttata ttttcactga aggcagcaac agcgtgcagg tcatcagccc ggatgggcaa
     8101 agccgacaaa aaatgcagtt ttactcaacg ccgggtacag gaacgattcg tgcacgttta
     8161 cggctggttc tctcgtggga tacggacaat accgatctcg atctccatgt tgtcacgccc
     8221 gatggcgaac acgcctggta cggtaacact gtgctgaaaa acagtggtgc actggatatg
     8281 gatgtcacga cggggtacgg acccgagatt ttcgccatgc cagcgccagt tcacggacgc
     8341 tatcaggtgt atatgaagtg gtcaacaaaa actggccacc gagttagagt ttttccagta
     8401 tcgattttcc
//
LOCUS       DAJTMK010000161         8349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTMK010000161 DAJTMK010000000
VERSION     DAJTMK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.165"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..255
                     /locus_tag="OO135_003320"
     CDS             <1..255
                     /locus_tag="OO135_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5399057.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO135_003321"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO135_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5399058.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO135_003322"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO135_003322"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5399059.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO135_003323"
     CDS             complement(2907..3182)
                     /locus_tag="OO135_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5399060.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO135_003324"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO135_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5399061.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAQLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO135_003325"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO135_003325"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5399062.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO135_003326"
     CDS             5142..5468
                     /locus_tag="OO135_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5399063.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO135_003327"
     CDS             5652..6371
                     /locus_tag="OO135_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5399064.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7592
                     /gene="ansB"
                     /locus_tag="OO135_003328"
                     /pseudo
     CDS             6547..7592
                     /gene="ansB"
                     /locus_tag="OO135_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
     gene            7708..>8349
                     /locus_tag="OO135_003329"
     CDS             7708..>8349
                     /locus_tag="OO135_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5399065.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKAFDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRD"
BASE COUNT         2165 a         2022 c         2206 g         1956 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaaaccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgagcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagctgcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccaaggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaag caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcagc tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagat ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgag
     7021 ctgataaagc atccgctaat cgtggcgtgc tggtggtgat gaacgacacc gtactggacg
     7081 gtcgcgatgt aaccaaaacc aacaccaccg acgtagcgac cttcaagtct gttaactacg
     7141 gtcctctggg atacattcac aacggtaaga ttgactacca acgtaccccg gcacgtaagc
     7201 acaccagcga tacgccattc gatgtctcta agctgaatga gctgccgaaa gtcggcatcg
     7261 tttataacta cgctaacgca tccgatcttc cggctaaagc actggtagat gcgggctatg
     7321 atggcatcgt tagcgctggt gtgggtaatg gtaacctgta taaatccgtg ttcgacaccc
     7381 tggcaaccgc cgcgaaaaac ggcactgcag tagtgcgttc ttcccgcgta ccgacgggtg
     7441 ctaccactca ggatgctgaa gtggatgatg cgaaatacgg cttcgtcgcc tctggcacgc
     7501 tgaacccgca aaaagcgcgc gtcctgctgc agctggctct gacgcaaacc aaagatccgc
     7561 agcagatcca gcagatcttc aatcagtact aatcacctcg ccccggtatc gtgccggggc
     7621 tttttcactt cagacttacg tccattgcca attttcatta ccctagtgat aatcacgaaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggct atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc
     8161 tttagtcagc ttgaatctgc gttgaatacc cagaagaact cgattcctgc gctggaaaag
     8221 gaagtgaaag cgtttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg
     8281 ggaaaagatg caaacggtaa gcaaatgacc cgcgaagaag cctttaaaaa aattcaccaa
     8341 cagcgtgat
//
LOCUS       DAJTMK010000162         8287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTMK010000162 DAJTMK010000000
VERSION     DAJTMK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.166"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..422
                     /locus_tag="OO135_003330"
     CDS             <1..422
                     /locus_tag="OO135_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HCR5399066.1"
                     /translation="AEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAADGLAVGRASG
                     FVGRAMVRLLDGFYTLSDQAMYDMLGWLAQEEGIRLEPSALAGMAGPQRVCASVSYQQ
                     MHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(530..2068)
                     /gene="emrY"
                     /locus_tag="OO135_003331"
     CDS             complement(530..2068)
                     /gene="emrY"
                     /locus_tag="OO135_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5399067.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2068..3231)
                     /gene="emrK"
                     /locus_tag="OO135_003332"
     CDS             complement(2068..3231)
                     /gene="emrK"
                     /locus_tag="OO135_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5399068.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            3647..4261
                     /gene="evgA"
                     /locus_tag="OO135_003333"
     CDS             3647..4261
                     /gene="evgA"
                     /locus_tag="OO135_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5399069.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4266..7859
                     /gene="evgS"
                     /locus_tag="OO135_003334"
     CDS             4266..7859
                     /gene="evgS"
                     /locus_tag="OO135_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5399070.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVDSPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEASITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKISMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPISDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(7915..>8287)
                     /locus_tag="OO135_003335"
     CDS             complement(7915..>8287)
                     /locus_tag="OO135_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HCR5399071.1"
                     /translation="FSALCQALELTELVNDPRFSSNILRVQNQAILKQYIERTLKTQA
                     AEVWLARIHEVGVPVAPLLSVAEAIKLPQTQVRNMLIEAGGIMMPGNPIKISGCADPH
                     VMPGAATLDQHGEQIRQEFSS"
BASE COUNT         2507 a         1802 c         1674 g         2304 t
ORIGIN      
        1 ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt ccatacagga ttacacgatc
       61 agatttctgt tcaggatatt ggtatcgaca accttaccgc agcggatggc cttgcagttg
      121 gtcgcgcatc aggctttgtc gggcgggcaa tggtgcgtct gctggatggc ttctataccc
      181 ttagcgatca agccatgtat gacatgcttg gctggctagc gcaggaagaa ggtattcgtc
      241 ttgaaccttc ggcactggcg ggtatggccg gacctcagcg cgtgtgtgca tcagtaagtt
      301 accaacagat gcacggtttc agcgcagaac aactgcgtaa taccactcat ctggtgtggg
      361 cgacgggagg tggaatggtg ccggaagaag agatgaatca atatctggca aaaggccgtt
      421 aataacgttt caacgcagca tcgcaatcct ttccctgggt gagcgatgct gccgatggcg
      481 cagacttaag atccccggtc ttacccgcta taaccccctc cttttatcat cacccaacgc
      541 ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac cgtaagtaag ataaaagcaa
      601 tagccgccat acggaaaatc tcatttgcag aaatagaaag tgactgctgg gttatttcat
      661 tattaatttc attaagaact cctgaaagcg aaccataata tttatccata atttgtgacg
      721 atgaattaaa cacggggtta aattgatcga tggttgctgt caactgacta tggtgtaacg
      781 attcgcgtcg tccccacagc gtcattgtca acgacgtacc aactgatcct gacaaggtac
      841 gaaaaaaatt actcatactc gaggcattgg caaatttatt atctggcaag cctgaaaacg
      901 aaatcgttgt taagggtaaa aagaaacagg caacggcgaa tccctgaaaa aactgcggca
      961 aaatgatgcc tgtaaaatca atcgttggca taaatgtcac agaacgccag taatagcaaa
     1021 ccgcatacat caaaaaacta aatgtcacta acaaccgcat gtctattttg ttgccataac
     1081 gtcctatcaa aggtgaaatt aatagtggca tgatgccgat gggcgcataa gcaagtccgg
     1141 cccatatcgc attatacccc atcgtttcct ggagtaactg cggcataagg acgatcgctc
     1201 cagagtaaaa taaatacgcg catgtgatac tcacaatacc aatggtgaag ttacgggact
     1261 taaacaaact gagatcaaga atcgggttct ctgaggtcga ctcccaaatg actaaagaga
     1321 tcagagaaat aactgatact actgttaata ttattattgt actcgagttg aaccaatcca
     1381 gatcgcgccc tttatcaagc ataatttgca agccaccaac accgagcact aacagggtca
     1441 gtcctggtag attcattttg accggtgaag tctcagtttc tcttccttta agtaaggtta
     1501 agcataatgt caggacgata atccccatag ggacattgat taaaaatatc caaccccagc
     1561 taaagttatc acaaatataa ccgcccaata tcggcccaca tatcggagcg ataatcacgg
     1621 tcattgacca taatgccaga gcaaatgttc ttttttctgg cggataattc cttaataata
     1681 aactctgtga cagtggaatt aacggccccg ccattaaccc ctgaacgact ctaaaaaata
     1741 tcagcacatc aagattggtc gataggctac acattaatga agacagcgaa aaaaaggtta
     1801 ctgaaagtaa aaataatctt aattcgccta ttctttgtgc caacctgcca gtaacaggga
     1861 tcgcaatggc atttgctaca ccaaacgagg tgataaccca ggtgccttcg tctgttgatg
     1921 ctcccagaaa gccagatatt gtcggtattg cgacgttaga aatagtggaa tccaacattt
     1981 gcataaatgt cgctaatgac aatgcaatag tgacgcacca taacgtccca ccggttaatg
     2041 gtgccggagt tgatttagtg attgccatta aagttgtcca ttatgcgaaa taatgttgct
     2101 aatttctttt tctatcggac tggtatcgat aactaaagcc ttactggtat aagccggcat
     2161 ggaggtcacg gttgaagcca gctcaggcat ctcggcaatg tcttcgttct tcgtatcaat
     2221 agttgctgtc atcgataaac caatacgcaa gggatgttcc atgagttctt ttggatcaag
     2281 agaaacttca accggtacac gctgaacgat tttgatccag ttccctgtcg cattttgtgc
     2341 aggtaataag gagaacgcat tgccggttcc catattgatc cctgtcaccc gaccatgaaa
     2401 cacaacattt tcaccataaa gatcgctgat aatattgacc gattgaccaa tccgcacatc
     2461 cgtgagttgt gtttctttaa agttggcatt aacccacatt tgacgtgccg gtacgacagc
     2521 cattaacgat tgtccggggc tcactgtttc gccgacctga acacttctct gggcaatata
     2581 gccggtaacc ggactcttaa tatccgtacg tttaagcgcc aaccaggctt ctttagttgc
     2641 atccgccgct tcaacgactt gtggctgacg gtttaatggt gtgttcatta ctaaagcttt
     2701 attcgcttta taagcctgga tagcggcatt caatgccgct ttgctactta ttaacgtatc
     2761 tttggtatgc tccagcgttt cttttgaaat aaccccctgc ttcgctaacg gcactcgacg
     2821 gttataatct tctaaagatt gttgatactg aatacgtgct gaagcgactt cggcactgta
     2881 ttgtttatcc tgtaagtata gtttattcgt ttgccgaaca atatttgcca gattattttt
     2941 agctttattg agtgcgatag tggcatcagt tttatccagt gaaactaaaa tgtcaccttg
     3001 tcgaacgtag ttcgtatctt tatgattaac gacagtgaca ctacctgaga cttgtgcaga
     3061 aattggatct gcattccccg tgacataggc gtcatctgta ctaatcatgt cttctaattc
     3121 cattgaccaa taggcatagg cacctgaaaa cgcaataaat aaaactaccg ccaataaaga
     3181 aaagtatttt cttctgttag aatgtttttt atttgaatta atctgttcca ctattatctc
     3241 tcatttctca tagatgaaat ttatgaattg aatgaaaaga gcattcattc ttcatatcaa
     3301 acacagtgca tgtgttgcac attatcccag gaacgttgga ggcctaattt aaatgaggtg
     3361 caaaaaaaac aacagaggac taaaccgtgg cttttgcaat acaattctta cgcctgtagg
     3421 attagtaaga agacttatag tgctaacttg aaactataaa tcatcggtac aacccctgat
     3481 tttattgttg acatttcatt tatgccgact atttatatgg tatacttgtc gaattatctt
     3541 aaaggaagct cagattttct tatttttatt gagaaaatga gatgacgcct tatgtctgta
     3601 ttactacagg gagaagggag atgcttcatt gcaaagggaa taatctatga acgcaataat
     3661 tattgatgac catcctcttg ctatcgcagc aattcgtaat ttattgatca aaaacgatat
     3721 tgaaatctta gcggagttga ctgaaggggg aagcgccgtt cagcgggtgg aaacacttaa
     3781 gcctgatatc gtcatcattg atgtcgatat ccccggagtt aacggtatcc aggtgttaga
     3841 aacgctgagg aagcgccaat atagcggaat tattattatt gtctccgcta aaaatgacca
     3901 tttttacggg aaacattgtg ctgatgctgg cgctaatgga ttcgtgagta aaaaagaagg
     3961 catgaacaat atcattgcgg ctattgaagc tgcaaaaaat ggctactgct atttcccctt
     4021 ctctctcaac cggtttgttg gaagtttaac gtccgaccag caaaaactcg actccttatc
     4081 gaaacaagaa attagtgtca tgcggtatat tcttgatggc aaggataata atgacattgc
     4141 tgaaaaaatg ttcatcagca acaaaactgt cagcacttat aaaagtcgcc tgatggaaaa
     4201 attagaatgt aaatcactga tggatcttta cacattcgcg caacgtaaca aaatcggcta
     4261 atcacatgaa gtttttaccc tatttttttc ttctctgttg tggtctttgg tcgaccataa
     4321 gtttcgcaga cgaagattac atcgaatatc gtggcatcag tagtaacaac cgtgtcacac
     4381 ttgatccagt acgtctgagc aacaaggaat tacgttggtt agcgagcaaa aaaaatcttg
     4441 tgattgcagt acacaagtcc caaacggcta ccttgttgca taccgattcg cagcaacggg
     4501 ttcgtggtat taatgctgat tatttaaatc ttttaaaaag agcgttaaat atcaaattaa
     4561 cactccggga atacgccgat catcaaaaag caatggacgc gctggaagaa ggtgaagtcg
     4621 atatagtgtt atcacattta gttgattcgc cacctcttaa tgatgacatt gctgcaacca
     4681 aaccactgat aattaccttt ccggcgctcg tcaccaccct tcacgattca atgcgaccgc
     4741 ttacctcatc aaaaccagta aatattgctc gagtagcaaa ttatccccct gacgaggtaa
     4801 ttcatcaatc atttccaaaa gcaacaatta tctcttttac aaatttatat caggcattag
     4861 catccgtctc agccggacag aatgattact ttattggtag taacatcatt accagcagta
     4921 tgatttcccg ctatttcact cactccttaa atgtagtgaa atattataac tcaccgcgtc
     4981 aatataattt tttcttgacc agaaaagaat ctgtcgttct taatgaagta ctcaatcgat
     5041 ttgttgatgc tttaacaaat gaagttcgct atgaagtttc acaaaattgg cttgatacag
     5101 gaaacctggc ctttctgaac aaaccattag aactcactga acatgaaaaa cagtggatta
     5161 agcaacatcc cgatttaaag gtgctggaaa atccttactc tcctccctat tctatgacgg
     5221 atgaaaatgg ctcggttcgg ggcgttatgg gggatattct taatattatt accttgcaaa
     5281 caggtttaaa tttttctccg atcaccgttt cacacaatat ccatgccgga acacagctta
     5341 accccggtgg atgggatata atacctggcg ctatttatag tgaagatcga gaaaataatg
     5401 tttcatttgc agaagcttcc ataacaacgc catacgtttt tgtcatgcaa aaagcacctg
     5461 acagtgaaca aacattaaaa aaaggaatga aagttgccat tccatattat tatgagctgc
     5521 attcgcaatt aaaagagatg tatccggagg ttgagtggat aaaagtcgat aacgccagcg
     5581 ctgcatttca caaggtcaag gaaggtgaac ttgatgctct ggtcgcgaca cagctaaatt
     5641 cgcgttacat gatcgatcat tactatccta atgaactgta tcattttctt attcccggcg
     5701 ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga accggaactt aaggatatta
     5761 ttaataaagc actgaatgca attcccccaa gcgaagttct gcgcctgacg gaaaaatgga
     5821 ttaaaatgcc caatgtgacc attgacacat gggacctcta tagcgagcaa ttttatattg
     5881 ttacgacatt atccgtttta ttagttggca gtagcctttt atggggattc tacctgttac
     5941 gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga aaaccaaata tcattccgaa
     6001 aagcactctc ggattcctta ccaaatccaa cttatgttgt aaactggcaa ggtaatgtca
     6061 ttagtcataa tagtgctttt gaacattatt tcactgcgga ttactacaaa aatgcaatgt
     6121 taccattaga aaacagtgac tcacccttta aagatgtttt ttctaatgcg catgaagtca
     6181 cagcagaaac gaaagaaaat cgaacaatat acacacaggt atttgaaatt gataatggca
     6241 tcgagaaaag atgcattaat cactggcata cattatgcaa tctgccagcc agcgatcatg
     6301 ctgtttatat ttgtggttgg caagatatta ccgagacgcg tgatttaatt catgcactcg
     6361 aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa aagtcagttt ctggcaacaa
     6421 tgagtcacga aataagaaca ccaataagct ccattatggg cttcctggaa cttctgtcgg
     6481 gttctggtct tagcaaggag caacgggtgg aggcgatttc acttgcctac gccaccggac
     6541 aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga caaaattgaa tcgggtaact
     6601 atcaacttca accacaatgg gtcgatatcc ctactttagt ccagaacact tgtcactctt
     6661 tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg cagcagtaca tttcctgatc
     6721 attacctggt taagatcgac cctcaggcgt ttaagcaggt cttatcaaat ttactgagca
     6781 atgctctcaa atttaccacc gagggggcag taaaaattac gacctccctg gttcacattg
     6841 atgacaacca cgctgttatc aaaatgacga ttatggattc tggaagtgga ttatcgcagg
     6901 aagaacaaca acaactcttt aaacgctaca gccaaacaag tgcaggtcgc cagcaaacag
     6961 gatctggctt aggcttaatg atctgcaaag aattaattaa aaatatgcag ggcgatttgt
     7021 cattagaaag tcatccaggc ataggaacaa catttacgat cacaatcccg gtagaaatta
     7081 gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc catcacacta cctgaaaagt
     7141 tgagcatatt aatcgcggat gatcatccga ccaacaggct attactcaaa cgccagctaa
     7201 atctattagg atatgatgtt gatgaagcca ctgatggtgt gcaagcgcta cacaaaatca
     7261 gtatgcaaca ttatgatctg cttattactg acgttaatat gccgaatatg gatggttttg
     7321 agttgactcg caaactccgt gagcaaaatt cttccttacc catctggggg cttacagcca
     7381 acgcacaggc taacgaacgt gaaaaagggt taagttgcgg catgaactta tgtttgttca
     7441 aaccgttgac cctggatgta ctgaaaacac atttaagtca gttacaccag gttgcgcata
     7501 ttgcacctca gtatcgccac cttgatatcg aagccctgaa aaataatacg gcgaacgatc
     7561 tacaactgat gcaggagatt ctcatcactt tccagcatga aacgcataaa gatctacccg
     7621 ctgcgtttca agcactagaa gctggcgata acagaacttt tcatcagtgt attcatcgca
     7681 tccacggtgc ggctaacatc ctgaatttgc aaaagttgat taatattagc catcagttag
     7741 aaataacacc tatttcagat gacagtaagc ctgaaattct tcagttgctg aactctgtaa
     7801 aagaacacat tgcagagctg gaccaggaga ttgctgtttt ctgtcagaaa aatgactaaa
     7861 tagcggctcc cacaatgttc aaatgtggga gctatttacc agcacatctt acatttatga
     7921 tgagaactcc tggcgaattt gttccccatg ctggtcgagc gttgccgctc ccggcataac
     7981 atgcgggtcc gcgcagccgc tgatttttat cggattaccc ggcatcatta ttcccccggc
     8041 ttcaatcaac atatttctca cctgagtttg tggcaattta atggcctcag ccacacttaa
     8101 taacggcgcg acgggtacac caacttcatg tattctggct aaccaaactt cagctgcctg
     8161 cgtttttaac gtccgctcaa tatattgttt aagaatagcc tggttttgta cgcgtaaaat
     8221 attgctgcta aatcggggat cattaaccag ttccgtaagc tccagtgcct ggcataacgc
     8281 agaaaaa
//
LOCUS       DAJTMK010000163         8271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTMK010000163 DAJTMK010000000
VERSION     DAJTMK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.167"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..1204
                     /gene="dcuC"
                     /locus_tag="OO135_003336"
                     /pseudo
     CDS             1..1204
                     /gene="dcuC"
                     /locus_tag="OO135_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(1247..1744)
                     /gene="sspB"
                     /locus_tag="OO135_003337"
     CDS             complement(1247..1744)
                     /gene="sspB"
                     /locus_tag="OO135_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5399072.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(1750..2388)
                     /gene="sspA"
                     /locus_tag="OO135_003338"
     CDS             complement(1750..2388)
                     /gene="sspA"
                     /locus_tag="OO135_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5399073.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(2783..3175)
                     /gene="rpsI"
                     /locus_tag="OO135_003339"
     CDS             complement(2783..3175)
                     /gene="rpsI"
                     /locus_tag="OO135_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5399074.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3191..3619)
                     /gene="rplM"
                     /locus_tag="OO135_003340"
     CDS             complement(3191..3619)
                     /gene="rplM"
                     /locus_tag="OO135_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5399075.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(3838..4965)
                     /gene="zapE"
                     /locus_tag="OO135_003341"
     CDS             complement(3838..4965)
                     /gene="zapE"
                     /locus_tag="OO135_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5399076.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFMPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5159..5557
                     /locus_tag="OO135_003342"
     CDS             5159..5557
                     /locus_tag="OO135_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5399077.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5711..7078
                     /gene="degQ"
                     /locus_tag="OO135_003343"
     CDS             5711..7078
                     /gene="degQ"
                     /locus_tag="OO135_003343"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5399078.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILASGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSAIAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7168..8235
                     /gene="degS"
                     /locus_tag="OO135_003344"
     CDS             7168..8235
                     /gene="degS"
                     /locus_tag="OO135_003344"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5399079.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         1992 a         2094 c         2135 g         2050 t
ORIGIN      
        1 gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac cgcattgccg
       61 gattggggct atcgattatg gcggtgggcg gtatgcccgc tacatggagc gcataggggc
      121 cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa ctcattcgct caccgtatat
      181 tattctgtcg gcaacttacg tcatcggtca aatcatggcg cagtttatta ccagcgcctc
      241 cggtctgggt atgttgctga tggtcacctt atttccgaca ctggtgagtc tgggagtaag
      301 tcgtctctct gcggtggcgg ttatcgcaac cacaatgtcc attgagtggg gaattctgga
      361 aacgaactcc atttttgcag cccaggtcgc gggaatgaaa attgccactt acttcttcca
      421 ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg gcgatctccc actttttcgt
      481 gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac gaacaagcag agcaaaaagc
      541 tctcgataat gtcccgccgc tctattacgc cattttacct gtgatgccgt taatcctgat
      601 gctcggctcg ctgttcctcg cccacgtcgg gctgatgcag tcagaactgc atctggtggt
      661 ggtgatgtta ctgagtttga cggtgacgat gtttgttgag ttcttccgca agcataactt
      721 gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc atgggtacgc agtttgccaa
      781 cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa ggcttaacga cgattggcac
      841 tgtcgatgcg gttatcaggg gtgcggagca ttctggtctg ggcggtattg gcgtgatgat
      901 tattatggcg ctggtcattg ccatttgtgc cattgtgatg ggctctggca atgcgccgtt
      961 tatgtcattt gccagtctta ttccgaatat cgcagccgga ctacatgtac cagcggttgt
     1021 aatgattatg ccgatgcatt ttgccacgac gctagcgcgc gcggtttcgc cgattactgc
     1081 ggtggtggtc gttacgtcag gaattgcagg cgtttcgcct tttgcggtgg tgaagcgaac
     1141 agcgatcccc atggcagtcg gtttcgtggt gaatatgatt gccacaatca cgctatttta
     1201 ttaagccatt aaaagacaaa acaggccacc agggcctgtt tttgtattac ttcacaacgc
     1261 gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc atcgtcagga tgagtgtcat
     1321 catcgtgatc tggcttgtcg ccatcaataa ccgacataac ggtttcgttg tctgccgatg
     1381 cctcttcatc attcatgatg ctggtatctt catcgtaggc agcttcaggc tcaaacattg
     1441 tgcctgcgcc attttcacgg gcgtagatag ccagcacggc agccagcggc acagaaacct
     1501 gacgcggaat gccgccaaag cgcgcgttaa agcgcacctc atcattcgcc agttccagat
     1561 tgccgacagc acgcggcgca atgttgagta cgatttgccc gtcacgcgca tattccatag
     1621 gaacctgcac gccagggagc gtcacatcca ccaccaggtg cggcgtgagc tggttatcca
     1681 gcaaccactc atagaatgca cgcagcagat agggacgacg tggtgttagc tgtgacaaat
     1741 ccatacagat taactccggc ccagacgcat ttcacgttct gcttcagtta aagaagcaag
     1801 gaaagagtca cgctcaaaga cgcgggtcat atagcctttc agctctttcg cacccgggcc
     1861 gctgaactcg atgcccagtt gcggcagacg ccacagcagc ggagcaagat agcaatcgac
     1921 caggctgaac tcatcgctca ggaagtacgg cttctgaccg aagaccggcg caatcgccag
     1981 cagttcttcg cgcagttgct tacgtgcggc atctgcttca gaagctgaac cgttgatgat
     2041 ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc atgtacagac ggctttcacc
     2101 gcgagctacc gggtaaacag gcatcagtgg cggatgcggg aaacgctcat ccagatattc
     2161 cataatgatg cgagattccc acagggtcag ctcacgatcc accagggtcg gaacgctctg
     2221 attcgggttg aggtcaatca gatcctgagg cggattgtcc ttttccacgt gttcgatctc
     2281 gaaacttaca cctttctcag ccagcacaat gcggacctga tggctataga tgtcagtagg
     2341 accggaaaac agcgtcatta ccgaacgttt gttggcagcg acagccatga aaacctccag
     2401 gtatagtcag aatttttact gctaccagcc accaggtggc cagtcagaag ttgtgttacc
     2461 cagtaaggaa cgactctctt tgttcgaaaa tcaaacaaaa aatgagcaat acccgacatt
     2521 tgggcagaaa attggatgat agtttaccag attttgcgac ctttgtggtg agtcgatgcc
     2581 ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt tgacttagtc ccttattggc
     2641 gatgtggttt ttgttttacc tgtctgtcag gtggcagcaa aaagcaactt tccagttttt
     2701 acgctgattc agattttagc tataaaaaaa cccgccgaag cgggtttttt cgaaaattgt
     2761 tttctgccgg agcagaagcc aattaacgtt tggagaactg cggacgacga cgtgctttac
     2821 gcagaccgac tttcttacgt tcaacctgac gagcgtcacg agtaacgaag ccagctttac
     2881 gcagttcaga acgcagggac tcgtcgtatt ccatcagagc gcgggtgata ccgtgacgga
     2941 tcgcaccagc ctgaccagag ataccaccac ctttaacggt gatgtacagg tccagtttct
     3001 caaccatgtc gaccagttcc agcggctgac gaactaccat gcgggcagtt tcacgaccga
     3061 agtactgttc cagagaacgt tggttgatta cgattttacc gttgcccggt ttgatgaaaa
     3121 cgcgagctgc ggaacttttg cggcgaccag tgccgtagta ttgattttca gccattgcct
     3181 ataatcccga ttagatgtca agaacttgcg gttgctgtgc cgcgtggttg tgctcgttac
     3241 ccgcgtaaac tttcagttta cggaacatag cacgacccag cgggcctttt ggcaacatgc
     3301 ctttaaccgc gatttcaatc acacgctcag gacggcgagc aatcatctct tcaaaggtcg
     3361 cttgtttgat accaccgatg tggccggtgt ggtgatagta cactttgtca gtacacttgt
     3421 tgccggttac agcaactttg tcagcgttca gaacgatgat gtaatcaccg gtatctacgt
     3481 gcggagtgta ttccgctttg tgcttaccgc gcaggcgacg agccagttca gtagccagac
     3541 ggcccagagt tttaccggtc gcgtcaacaa cataccagtc gcgttttacg gtttctggtt
     3601 tagctgtaaa agttttcatt aaaagcttac ccaataaata gttacacgtt ggtgaacacc
     3661 caaacgtctt caattgttga ggttcacacg acaaagtccg gcaaacctac cccttcgaat
     3721 agcctatgcc agcacacaaa aagttttggg aaaaaaactt tcttgtaacg tggggtcgca
     3781 ggattataga gaagtcgggc tcaaagatcg accccttttt gtgatttgtg acaggtttta
     3841 acccgccaaa tgctcgcgct tcagatactc ttcgctttgc atctcttgca gacgtgacag
     3901 gcaacgctgg aactcaaact tcagccgatc gccctgataa atttcataca gcggcacttc
     3961 tgcactcacc actaatttga catggcgctc gtaaaactca tccaccagcg caataaagcg
     4021 ccgcgcttcg ctctccatca accgcgtcat aactggtaca tcaaacaaca tgaccgtatg
     4081 aaagaggcgt gagagcgcaa tatagtcatg ctggctgcgg gcatcgacac acaaggtggt
     4141 aaaagagaca gcaagcgtct gattctcagc gcccattgtc gccagcggtc gatggttgat
     4201 ttctaacgtc ggtgaatttt cgcgtttcgc tcccgccagc gccaaccata gtttatccat
     4261 ttgtgcccgg gtttcatcgt ggagtggcga aagccacaga tgcgcctgag tgagtgtacg
     4321 cagtcgataa tcaacaccag cgtccacgtt cattacatca caatgctgtt taatggcatc
     4381 gattgcaggc ataaaacgcg cacgttgcaa gccatttcga taaagttcgt ccggcggaat
     4441 atttgacgtc gctaccaggg taataccgcg ggcgaacagg gctttcatca gaccgccaag
     4501 tagcatggcg tcggtaatat cagaaacaaa aaattcgtca aaacagagca cgtcagtttc
     4561 ggctttaaag cgatcggcaa taatttccag cggatcggtc tgcccctgta aggcagttag
     4621 ctcttcgtgc acccgcagca taaaacggtg aaagtgcagg cgctgtttcc gctctcccgg
     4681 caggctttga tagaaaaggt ccatcagcca ggttttcccg cgtcctacac cgccccacat
     4741 atataagcca cgcactggcg tatgctttgt gtcttcgcgt ttaccccaca gcttaccgac
     4801 ccgcgccatt agcccactcg tcctgggggc tggtggcctg ctattgatga gttcttgata
     4861 aataatttcc aggcggctga cggcctcttt ttgaacgtcg tcgggttgat ggctgccttc
     4921 attgagcgcc ttcaggtatt gcgatgttgg ggtaacgctt tgcatgatct tattattatt
     4981 ccttgaataa tcggtgcgcc gttgttcacg gttgacgaaa aaaaggccgt tctacactac
     5041 gcgatatgca gtcgggattc cacttctgtg gaattaacgg ttatagtggc ataatcagcc
     5101 gcaggcatgg agcctgaagc caacacccta cggaaacaaa agacaacggg agatgttcat
     5161 gacctgggaa tatgcgctaa ttgggttagt cgtcggcatc attattggtg ctgtggccat
     5221 gcgttttggt aatcgtaaac tacgccagca acaggcgttg cagtacgaac tggaaaagaa
     5281 taaagctgaa ctggacgagt atcgcgaaga gctggttagc cactttgccc gcagcgcgga
     5341 attactggat accatggcgc acgactatcg ccagctgtat cagcacatgg cgaaaagctc
     5401 cagcagcctg ctgccggaac tgtctgctga agcaaacccg ttccgtaatc gtctggcaga
     5461 gtctgaagcc agcaacgatc aggcaccggt gcagatgcct cgcgactatt ctgaaggtgc
     5521 atccggcctg ctgcgtactg gcgcgaagcg cgactaattt atttttcggg cgcagccatt
     5581 gcgccctcct cttctctccc tccccgacta tcatttaatc tggtgtctca ttgttagccg
     5641 tctgaaaatt caataacatc aaactgtttt gaatctcttt tcttatcatt caggtacgag
     5701 agcaggaata atgaaaaaac aaacccagct gttgagtgca ttagcgttaa gtgtcgggtt
     5761 aactctctcg gcgtcatttc aggccgtcgc gtcgattcca ggccaggttg ccgatcaggc
     5821 ccctctcccc agtctggctc caatgctgga aaaagtgctt ccggcagtgg tgagcgtacg
     5881 ggtggaagga acggccagtc agggacagaa aatcccggaa gaattcaaaa agttttttgg
     5941 tgatgattta ccggatcaac ctgcacaacc cttcgaaggt ttaggttccg gtgtcatcat
     6001 caacgccagt aaaggctatg tgctgaccaa caaccatgtg attaatcagg cacagaaaat
     6061 cagtattcag ctcaatgatg ggcgcgagtt tgatgcaaaa ctgattggta gcgatgacca
     6121 gagcgatatc gccctgttac aaattcaaaa cccgagcaaa ttaacgcaaa tcgctattgc
     6181 cgactccgat aaattgcgcg tcggtgattt tgccgtagcg gtcggtaacc catttggcct
     6241 tgggcaaacc gccacctctg gcattgtttc cgcattaggc cgcagcgggt tgaatcttga
     6301 aggtctggaa aactttatcc agacagatgc ttccattaac cgcggtaact ccggcggtgc
     6361 actattaaac cttaacggtg agttaattgg catcaacact gcaatccttg cgtctggcgg
     6421 cgggagcgtc gggattggat ttgccatccc cagtaatatg gcgcgaacac tggcgcagca
     6481 gcttatcgac tttggtgaaa tcaaacgcgg tttgttagga atcaaaggca ccgagatgag
     6541 tgccgatatc gccaaagcct tcaaccttga cgtgcagcgt ggcgcgtttg tcagcgaagt
     6601 gttgccaggt tctggctcgg caaaagcggg cgtcaaagcg ggcgatatta ttaccagcct
     6661 caacggcaaa ccgctgaata gctttgctga gttgcgctct cgtatcgcga ctacagagcc
     6721 gggcacgaaa gtgaagcttg gcctgctgcg taacggcaaa ccactggaag tagaagtgac
     6781 gcttgatacc agcacctctt cgtcggccat cgctgaaatg atcacgccag cgctggaagg
     6841 tgcaacgttg agcgatggtc agctaaaaga tggcggcaaa ggtattaaga tcgatgaagt
     6901 tgtcaaagga agcccagctg ctcaggcagg cttgcaaaaa gacgatgtga tcattggcgt
     6961 caaccgcgat cgggtgaact cgattgctga aatgcgtaaa gtgctggcgg caaaaccggc
     7021 catcatcgcc ctgcaaattg tacgcggcaa tgaaagcatc tatctgctga tgcgttaatg
     7081 tcgtaaaccg ggcatcaggc ttacgtgtga tgtccggtta actcgtggta tgctgctgcc
     7141 gttccctttt ttaatgacgc ctccatcatg tttgtgaagc tcttacgttc cgttgcgatt
     7201 ggattaattg tcggcgctat tctgctggtt gccatgcctt cgctgcgcag ccttaacccg
     7261 ctttccactc cgcaatttga cagtaccgat gagacgcctg ccagctataa tctggcggtt
     7321 cgccgcgccg cgccagcggt ggttaacgtt tacaaccgtg gtgtgaacac caactctcac
     7381 aaccagcttg agatccgcac cctgggatcc ggtgtaatca tggatcaacg cggttatatc
     7441 atcaccaata aacacgtcat caacgacgcc gatcagatca tcgtcgcctt acaggatgga
     7501 cgtgtatttg aagcattgct ggtgggatct gactctctaa ccgatctggc ggtacttaaa
     7561 attaatgcca ctggcggttt acctaccatt ccaattaatg cacgtcgcgt accgcacatt
     7621 ggcgacgtag tactggcgat cggtaacccg tacaacctcg ggcagaccat tacccaaggg
     7681 attattagtg ccacgggtcg aatcggtctg aaccctaccg ggcggcaaaa cttcctacaa
     7741 accgatgctt ccattaacca cggtaactct ggcggcgcgc tggtgaactc gctgggagaa
     7801 ctgatgggca ttaacacgct gtcgtttgat aagagtaacg atggcgaaac gccggaaggt
     7861 atcggctttg cgattccttt ccagttagca accaaaatta tggataagct gatccgcgat
     7921 gggcgcgtga tccgcggcta cattggcatt ggtggacgtg agatcgcacc actgcacgcg
     7981 cagagcggtg gtatagatca actgcaaggg atcgtggtta atgaagtgtc acctgacggc
     8041 ccggcggcga atgcgggtat tcaggtcaac gatctgatta tttcagtgga taacaaaccg
     8101 gccatctctg ctctggagac gatggatcag gtggcagaaa ttcgccctgg ttcggtgatc
     8161 ccggttgtag tgatgcgtga tgataagcag ttaacgctgc aggtcaccat tcaggaatat
     8221 ccggcaacca attaagtcga gtgctcaaaa caaaaaaccg gagtctgtgc t
//
LOCUS       DAJTMK010000164         8236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTMK010000164 DAJTMK010000000
VERSION     DAJTMK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8236
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.168"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(9..401)
                     /locus_tag="OO135_003345"
     CDS             complement(9..401)
                     /locus_tag="OO135_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5399080.1"
                     /translation="MRDIQMVLERWGAWAANNHENVSWSPIAAGFKGLIPSKVKYRPQ
                     CCDDDGLIISSVMAVLKKKEPYQYQLLEMYYVYGITLRALGAKMGISLNQVVIRLQKA
                     EGFVEGCLAMLEVSLEMDCYMNHENDSR"
     gene            complement(419..1408)
                     /locus_tag="OO135_003346"
     CDS             complement(419..1408)
                     /locus_tag="OO135_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5399081.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDGLHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            complement(1460..1717)
                     /locus_tag="OO135_003347"
     CDS             complement(1460..1717)
                     /locus_tag="OO135_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399082.1"
                     /translation="MNNRTITVSPEQLRRQAQEMLRCAEQMEKTSVKKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(1714..3114)
                     /locus_tag="OO135_003348"
     CDS             complement(1714..3114)
                     /locus_tag="OO135_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5399083.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFF
                     VRQYRDIYAGICRQARISGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAYTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVGRVECRNQGLEKSRTLMTGINELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSTAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRYHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPDTQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(3111..3989)
                     /locus_tag="OO135_003349"
     CDS             complement(3111..3989)
                     /locus_tag="OO135_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5399084.1"
                     /translation="MMTIDQREKQTRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGKYRTHILVWIDRNGEDREKISCCPDCLIAEASDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIVDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYLSRIGGVAA"
     gene            complement(4000..4824)
                     /locus_tag="OO135_003350"
     CDS             complement(4000..4824)
                     /locus_tag="OO135_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399085.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNYITALCESGLVKKVSRKGEKGNSSNIYLLRLDGAKDSPGGSANNSL
                     PSAANSPGGVGVAPGGSAGDSPRTSHSFEPVKEPVNESTIGASADASAPARSARQEYS
                     PEFEQAWQEYPKRAGGNSKSAAFKAWKARIREGIKPETMLDGVKRYAAWVRATGNTGT
                     QFVKQAATFFGPDRHFEDYWQQPAAHGGGRQRQVDVLAGLGAMSDKFGKSSNKLTF"
     gene            complement(4821..5045)
                     /locus_tag="OO135_003351"
     CDS             complement(4821..5045)
                     /locus_tag="OO135_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399086.1"
                     /translation="MIRHIVNSLYHRYNRCPRVGQWFATSNGHVLRVCLVNAESQKVV
                     CELQGRSYTISYPLAVFLSGKMFKRLGGVL"
     gene            complement(5042..5236)
                     /locus_tag="OO135_003352"
     CDS             complement(5042..5236)
                     /locus_tag="OO135_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399087.1"
                     /translation="MISYEIIISTTEYRNDVSVRTDVSVWHRRYKSRKTAELKAAEMC
                     ETISMKGSPVKYVTTAEVRP"
     gene            complement(5233..6084)
                     /locus_tag="OO135_003353"
     CDS             complement(5233..6084)
                     /locus_tag="OO135_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5399088.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKVGAGGRSPELSTAIYDAPASF
                     LSSALTHARIMVGWAGEPKGSPVSVDAGTANPVQSATSEFRSSGGGSFPQSTEAAIMA
                     TIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTLECSASFTARNFSVSDY
                     TDCTGRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMEKNLSGADAVDMSA
                     VARNARGVYLHLREIHQIWTSQLYPMLKAIESPLASKLYDRVGDAVFGAALVDSRLNG
                     SDKEVRP"
     gene            complement(6193..6900)
                     /locus_tag="OO135_003354"
     CDS             complement(6193..6900)
                     /locus_tag="OO135_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5399089.1"
                     /translation="MNTAIFNGKASMTSVEIAELVGKRHDNVKRTIETLAKGGVVRSP
                     QIEVSERINNLGFKVQYEHYLFEGEQGKRDSIIVVAQLCPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVSHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            7236..7892
                     /locus_tag="OO135_003355"
     CDS             7236..7892
                     /locus_tag="OO135_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399090.1"
                     /translation="MKSLGERLINARQKAGLTQDALAKKAGITRVAISKAEQGLTKSF
                     NGDTLFKVAAALRCSPQWLQNGDEKDKHWENNVKSCPQRDTAHSYPVINWVQAGLFAT
                     SGDDYNMYDQDNWRHSVKYAGERGFWLEVHGDSMTSPVGITFPEGMSILVNPDKEVFS
                     GCYVIARKKSTNEATFKKYISDMGKAFLKPLNPQYPIIEMDNDCEIVGVVVDARWDIF
                     "
BASE COUNT         2056 a         2212 c         1886 g         2082 t
ORIGIN      
        1 ctgttcattt atcttgaatc attttcgtga ttcatatagc aatccatttc taaagatacc
       61 tcgagcattg ccagacaccc ttcaacaaac ccttcagctt tctgcaatct aataacaact
      121 tgattaagcg atatccccat tttcgcccct aacgctcgta atgtgatccc atatacataa
      181 tacatttcca gtaattggta ttgatatggt tcctttttct tgagaactgc cataactgaa
      241 ctaatgataa gaccatcatc atcgcaacat tgcggtcgat attttacttt tgaggggatg
      301 aggcctttaa accccgcagc aattggcgac caactaacat tctcatgatt atttgccgcc
      361 catgctcccc agcgttcgag aaccatctga atatcacgca tcaactttct ccacaaaatc
      421 aggacagcac acctatcgcc agcgcgcgat cgataaaacg aaatatcagc tccagttggg
      481 aaccatactt ctcttcaaat gccacggtat ccgcatgcag tccgtcatgg tgttttctgc
      541 acaaaggcaa cacaaaaagg tcatgcgctt ttgtacccat tccaccctga ccatgaccaa
      601 tcaggtgatg aggatcgtcg gctggcttac cacaacatgc acacggctgc gtcttaaccc
      661 agcgcgtgta cttttcatta acccagcgac gacgtttggg gcgtaacata aaagactccg
      721 gcgactccgg atccactttc agcgccagca cctttttcgc cttatcccgg atgatgctgg
      781 tggcaggaac cgaaggcaca aggtcacttt cccgggtgac agacggcaca acaggcttcg
      841 gtaatctcag tgccttacgg gctgcacttt ccggtaaggc atccgccagg tcattacgaa
      901 ccagccacca gcacagttcc ggcattgtca caacgtggct gtcatcaaaa ccgagatcac
      961 gacggactac ggacaacacc cagcgggcac agttatccgt tgccattgat tccagacgtt
     1021 ccgtgaactg atcgcgaagc aggttatcgc agtgccagca cagacggatt gcgcccggcg
     1081 cgtgtcgcat tgtggtcatg ttctcgctgt gccatccgga atgaggccac tggcagcctt
     1141 tttcacgaag taaccagctc tcaaggcatt ccacgccacc agcacgacgg atcaccgcct
     1201 catggcggaa cacggcccga acggcaggat catccgccag cggttgtgat gctgccggaa
     1261 cggcaccact ggcaaaagat gaataacgtt ccggctcagg ctccagcagg acacgcccct
     1321 gcataaacag gggcatcagc tctgaaccgg gtctgaacaa gacgatcccc atacgcgggg
     1381 caatttcagg ggtcagcagt gctctcacgg tcacctcagc aaacgatatt gaatgcatac
     1441 agagaaaaaa actcagtcat cacgcagtaa actccttcac cagcgtttca aactggctta
     1501 cctgtccttc cagttccgcc acgcaatcca ccagctcatc caccgccttt tgtgtgcggt
     1561 gttttgcctg cagcagatca cgaagcgccg gagtaagctg cttgcggagc gtatcttttt
     1621 tcacgctcgt tttttccatc tgttcagcac aacgaagcat ctcctgcgcc tgccgacgaa
     1681 gttgttccgg tgaaacagtg attgttctgt tgttcaaaat aaacgctccg ttttactgcc
     1741 cgacatgcgg ttattgctgt atctgcgcgg attgcccggc gtcatgggtg tggaaagaac
     1801 ccgggcactc tcctggtcca caggcagaaa atgtccgtta tgaaaacgcc ggtaaatggt
     1861 cccgagtgtg ccattacgct gtttcgtgat gttgatttct gctatgcctc tggcctgtgt
     1921 atccgggtta tacacctcat ccctgtaaag catcagaatg atgtcggcat cagcctctat
     1981 ttccccggag tttttcaggt ctgagttcat gggacgttta ttgggtctgg attccacgcc
     2041 tcgggagagc tggctcagag caatcagcgg gaaaccgccg gattttgcca ggcttttaag
     2101 tccctttgag atttccccca cagcaaggtc gtgacgcccc gtgctgcggg ttttaatcag
     2161 accgaggtaa tcgaccacca ccagcgccgt ttccggatgc ttcatccggt ggtacctcgt
     2221 ggttgcacat atctcatcaa ttgttagatt tgcctggtcc accatccaga tattacgccc
     2281 cgtcattcgt cccacgccct gcgagaaacg agcccagtct tcatcttcaa aacgggcaac
     2341 agacttaaga cgggataccg gcattccacc ggcagtagac accatacgtt caccaatctg
     2401 gatgttcgcc atctccatgg tgaacagaag cacgccatgc ccctgctcag tcaccttgtc
     2461 gatgatgtcc agcgccagtt cggttttccc catcgaagga cgagccgcaa tgaataccag
     2521 gtcgcctggc tccatccccc ctgtttttgc gtccagttca ttaataccag tcatcagtgt
     2581 tctggatttc tccagtccct gattccggca ttcaacacgg cccaccactt ccggaaggac
     2641 atcatcaatg tgaaccggct gaatgacgcc ctttccggtc gacagtgagg ccatcatgtt
     2701 ctgcgcatcc ttcagggcat cctcggctgc ttcacaggta tacgcatcac gtaaattctg
     2761 taatgcttca gtcagtgttt tttctgcatc acgcagtgcg gcattgcgcc gcaacgctgc
     2821 gacatagtgc tccagtgaag acttcaccca ggttttgcgt ccggtgtcgg taatcaccgg
     2881 ggcaagttcc ggcatctcat tgcacagcag tacggggtca atgacgccgg atatgcgagc
     2941 ctgtctgcaa atccccgcgt aaatatcccg gtactgacgc acaaaaaata catccgccgg
     3001 aagtgtggcc agaatatcca tcacttccgg atcggcccca cgcagaaaaa acgcaccgat
     3061 gacagcgcct tccaggtcat cgttacgcca tgccggggtg ttctggctgg tcatgcggca
     3121 acacctccga tacgagaaag gtagctgggc cagttaaacg acaaccagtt gcgcccgcca
     3181 tcggtgatcc tgtcggcaat ccggggactg atgaacgccc acaattcttc cggtgaaagg
     3241 ttgctgatca ggatagttgg caaaataccc tcataccggg cattgataat ttcctgcaaa
     3301 atggccattt cagccgcact gccaaactga acgccgactt cgtcgacaat cagcaaatcc
     3361 agtgacgcat aatgctcaat gacgtcatcc gctgtttttt cactgtcatt ccgccagcag
     3421 tttttcacag cccgggtaag gcgcatcacg tcggtgatct ccacactggc cagatagtta
     3481 cggatgatgt gttttgccat tgataccgcc agatgatttt tcccggtacc gcaactgccg
     3541 gtcataacaa gactggtacc gttctccagc atatctggcc agttctccgc atagcggcga
     3601 caggccgcaa gatttctggc tgcgtcagga ttaacctcca gataattatc aaactcgcag
     3661 tcccgaaaac gcagagcaat tccggcgtta tcagtcagtt cttccgcctt aagggacgac
     3721 agttccatgg tcaaatcact ggcctcagcg atcaagcagt cagggcagca tgaaattttt
     3781 tctctgtcct cgccattacg atcgatccac accagtatat gcgtacgata tttaccgtgt
     3841 ttttcgcaat atccgcgacc ttcacgcatc aggcaggaac gataaggcca tggcttttcg
     3901 cccttctgag caaatgcaat ctctgcccgt aactcatcca ttcgcgcctg tagtcttgtt
     3961 tgtttctcac gttggtcaat cgtcatcatc gctgtcacct cagaatgtca atttgttact
     4021 ggatttaccg aatttgtcag acatggctcc caggccagcc aggacatcga cctgtcgctg
     4081 tcgcccacct ccgtgagcgg ctggctgttg ccagtaatct tcgaagtgac gatcgggtcc
     4141 aaagaacgtc gcagcctgct tcacgaactg tgtgccggta tttcctgtag cacgtaccca
     4201 ggcggcatac cgcttcacgc catcaagcat ggtctccggt tttattccct ccctgatacg
     4261 ggctttccag gctttgaagg ctgctgactt ggaattgcca ccagcacgtt tgggatattc
     4321 ctgccaggcc tgttcaaatt ccggtgaata ttcctgtctg gcagaacgcg ctggtgcaga
     4381 cgcgtcagcg gatgcgccaa tagttgattc attgactggt tctttgactg gttcaaaaga
     4441 gtgactggtt ctgggtgaat ctcctgcact accccctggt gcaactccta caccacctgg
     4501 tgaatttgct gcactaggta gtgaattatt tgcactacca cctggtgaat cttttgcacc
     4561 atcaagacga agaagataaa tattgctcga gttccctttt tcacctttcc gggaaacttt
     4621 ttttaccagt ccagattcac aaagagccgt aatataattc atcacagaac gtttgctaat
     4681 ctcgcactga tcagcaatat gctgatagct gggccagcac tcgccctgat cgctggcatt
     4741 atcagccagc ttaatcagaa ccagttttcg caacggattt cccacacgaa ttttcatcgc
     4801 tttcaccatc agctccatgc tcataacaca cctcccaggc gtttaaacat ttttccagac
     4861 agaaataccg ccagagggta actgatggtg tagctacgcc cctgtagttc gcacacgact
     4921 ttctggcttt cagcgttgac taggcaaacc cgcagaacgt gaccgttgct ggtggcgaac
     4981 cactgcccca cacgggggca acggttgtat cggtgataca gggaattaac gatgtggcgg
     5041 atcatggacg cacctccgcc gtagttacgt atttaaccgg gctacctttc attgagatgg
     5101 tttcacacat ctctgccgct ttcagttccg ctgtttttct ggatttatag cgacggtgcc
     5161 agacagatac atccgtgcga actgatacat cgtttctgta ttccgtagtg gagatgatga
     5221 tttcgtaact aatcatgggc gaacctcctt gtcagaacca ttcagcctgg aatcaacaag
     5281 tgcagcgcca aaaacagcat caccaacacg gtcgtacagt ttgctagcca gcggagattc
     5341 aatggcctta agcattggat aaagctggct tgtccagatt tgatggattt cacgcaaatg
     5401 caggtatacg cctctggcgt ttcgtgcgac agctgacata tcaaccgcgt cagcaccaga
     5461 taaattcttc tccatctggt taaaggcgtt gatgtatgcc tctttgaacc gggctgcacg
     5521 tttgccagta aagcccatag caaggaacgc gaagccatca cgggtgattt gatagcaagg
     5581 tagtttgcgg cctgtgcaat cggtgtaatc actcaccgaa aaattgcggg cagtgaatga
     5641 tgcggagcat tcaagcgtgc ggatcttttt cagtacatcg tcatgacgct tggagaagaa
     5701 gttggcaaca gccagggatg aagtaacagc ctgaccatca acgatggcaa tttcaggttg
     5761 agtgagggtt gggatcgtag ccatgatggc agcctccgtt gactgtggaa aacttccacc
     5821 accggagctg cgaaactcac tggtggcaga ctgaacaggg ttcgcagtac cggcgtcaac
     5881 ggagaccggc gagcctttcg gctcccctgc ccagcccacc ataattctgg cgtgcgtgag
     5941 cgcggacgat aaaaaagacg ctggcgcgtc atatatcgcc gttgacaatt ccgggctgcg
     6001 acccccggca cccactttat aaggtgcaga gacagtgtaa cgtcccgaaa ttgcagaatc
     6061 aatatttggt cttgaaatga tcatatagct gctgatatct ttagaactgt tcttggatgt
     6121 ttcggagccg ttttatgcga aacagctccc cgttattgat gttgagtgag ccgggttact
     6181 cccggctttt tttcaccgct gccaaccaat aacctgaaat aaccccattt tcgggtgata
     6241 ccagcgagtc cctcgcggtt ctgcttcctc cataacccga taaaaagcag ccataaacgg
     6301 ttccacagca acaattgcgc gacgtgacaa caatccgtcc ggcgtcatga actcatgggt
     6361 gtctgtagga atctgatagg cgttcaccag attgcggcat ttatcatctg acaaaccggt
     6421 ttttgctttc agttggcgat atccggcata gccctcacga atagtgccct ttttaatttg
     6481 ctcgactgtt tcagcaacgt ggctgacttt ttcttccacc tgagtgatcc gtttctgctg
     6541 acgaactgct tcaagagcca tcgcggcaac catttcgatt tcgctcattg gcttacggat
     6601 ctgttcttcc agttcgcgcc agcgatctac caggcgagca gtgaattcag gacagagctg
     6661 tgcgacgaca atgatgctgt cgcgcttacc ttgttctcct tcaaacaggt aatgctcata
     6721 ttgaacttta aaacctaagt tattgattct ttcggaaacc tcaatttgag gagaccggac
     6781 aacgccacct ttggctaatg tttcaatagt gcgtttcaca ttgtcatgac gtttacccac
     6841 cagctctgcg atctcaacgc tggtcatgga tgctttgccg ttaaaaattg cggtgttcat
     6901 tgttggtctc ctgtgggctt gtcatcttct gtattcgcta gacttgggtg tgtatatgga
     6961 atgctcggat ccagatgaca aagaatggca acatcctccg gaacacctcg cgttttccac
     7021 tttccaacac cttgactgcc acgaggcctt cctttctttg ggaacctgcg accaatagcg
     7081 gcattggttt taaattgaat ttttaatatt tcataaaggg tcattcttta gtctcacacc
     7141 agatactttg ttatccaacg atgttaaccg caagaatcca aagtatcaag atattctgtt
     7201 actttagtat caacagccat gagaggagaa gaaaaatgaa gtctttaggt gaacgtctca
     7261 tcaacgcacg gcaaaaagct gggttaacac aagatgcgtt ggctaaaaaa gctgggatca
     7321 ccagagttgc aatcagtaaa gccgagcaag gccttacaaa aagtttcaac ggtgacaccc
     7381 tttttaaagt tgcagctgca ctgcggtgtt caccgcagtg gcttcagaac ggagatgaaa
     7441 aagataagca ttgggaaaat aatgttaaga gctgcccaca gagagacaca gcacactctt
     7501 accctgtaat taactgggtt caggcaggat tattcgcaac ttctggtgat gactacaaca
     7561 tgtatgatca ggataattgg aggcattctg taaaatacgc tggtgagagg gggttctggc
     7621 tggaagtgca cggagactca atgacttcgc ccgtaggaat aacatttcct gaaggaatgt
     7681 cgatccttgt caacccagat aaagaagttt tttcagggtg ttacgtcatc gccagaaaaa
     7741 aatccaccaa tgaagcaaca ttcaaaaaat atatttctga catgggaaag gcgtttctaa
     7801 agccccttaa tccacaatat ccaatcatag aaatggacaa tgattgcgaa atagtaggtg
     7861 ttgtggttga tgccaggtgg gatattttct gaccagacac aaagcacaaa aagaaaacaa
     7921 agtatcaaaa atcacttgtc acaccttgat accttagtta ccataaaaca aagttcgtaa
     7981 ctgaggtatc atctcatgtt caataaagct acaactcttg actgtctcga agaactgaaa
     8041 aacctcggca gcctcattac actaatagca aaagcaacac ctgatgctac gctctctagc
     8101 gatatagagt catgcgcagg actggcatgg gatatgacaa atagcatatc cagaaagcta
     8161 tcgtcagcaa tgcttttaca gaacaaaaat tctgcaatca acaaccgtct tcgcacccaa
     8221 cgcgaagcct gcggct
//
LOCUS       DAJTMK010000165         8100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTMK010000165 DAJTMK010000000
VERSION     DAJTMK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8100
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.169"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(574..948)
                     /locus_tag="OO135_003356"
     CDS             complement(574..948)
                     /locus_tag="OO135_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399091.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(960..1112)
                     /locus_tag="OO135_003357"
     CDS             complement(960..1112)
                     /locus_tag="OO135_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5399092.1"
                     /translation="MDTIDLGNSESLVCGVLPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LARNSD"
     gene            complement(1302..1709)
                     /locus_tag="OO135_003358"
     CDS             complement(1302..1709)
                     /locus_tag="OO135_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399093.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            1787..2014
                     /locus_tag="OO135_003359"
     CDS             1787..2014
                     /locus_tag="OO135_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5399094.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            1998..2549
                     /locus_tag="OO135_003360"
     CDS             1998..2549
                     /locus_tag="OO135_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399095.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRKKFTALIPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGLYEYRCTTMG"
     gene            2521..3561
                     /locus_tag="OO135_003361"
     CDS             2521..3561
                     /locus_tag="OO135_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5399096.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNGTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPLVFLDGLSEPIGKYPMTDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            3473..4015
                     /locus_tag="OO135_003362"
     CDS             3473..4015
                     /locus_tag="OO135_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5399097.1"
                     /translation="MGEVYSNRWTQKNGAAPSKLWIAQIGAMTEQQIRQVCRQCMDRC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRDESWRYDGSDKYPWPQPV
                     LYHICLEMRTRGIERQMTQGELKRLAERQLTKWAKHVGNGMSVPPVRRQLEGAKHPQG
                     PTPIERLKQEYERRKAAGFI"
     gene            4049..4819
                     /locus_tag="OO135_003363"
     CDS             4049..4819
                     /locus_tag="OO135_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR5399098.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            4835..5227
                     /locus_tag="OO135_003364"
     CDS             4835..5227
                     /locus_tag="OO135_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5399099.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5224..5520
                     /locus_tag="OO135_003365"
     CDS             5224..5520
                     /locus_tag="OO135_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5399100.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            5517..5978
                     /locus_tag="OO135_003366"
     CDS             5517..5978
                     /locus_tag="OO135_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5399101.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLKEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            5956..6312
                     /locus_tag="OO135_003367"
     CDS             5956..6312
                     /locus_tag="OO135_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399102.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRKNIEKLIQIE
                     RNAKARICRATKSRTE"
     gene            6408..6590
                     /locus_tag="OO135_003368"
     CDS             6408..6590
                     /locus_tag="OO135_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399103.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIQDEAKDIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            6583..6759
                     /locus_tag="OO135_003369"
     CDS             6583..6759
                     /locus_tag="OO135_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399104.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            6756..7115
                     /locus_tag="OO135_003370"
     CDS             6756..7115
                     /locus_tag="OO135_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399105.1"
                     /translation="MSEINYQEGHEKAGQAKPVAWRYRYVKKGVTDFQGKQWVGDWKY
                     VPTKEDCNDRPNYEIQALFTAPPASVTSEGLVKAVRFYEQVRREDPPVETGAWKDAID
                     WVLKEACLVVNTGIKGG"
     gene            7116..7331
                     /locus_tag="OO135_003371"
     CDS             7116..7331
                     /locus_tag="OO135_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399106.1"
                     /translation="MGIAASYTMHLYCDCRQCTDGKYQSPDFGEYMGTSWAGCAKEAR
                     KDGWRISADKTRAFAPGHKILRSNKGE"
     gene            7333..7551
                     /locus_tag="OO135_003372"
     CDS             7333..7551
                     /locus_tag="OO135_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5399107.1"
                     /translation="MPTLFRKEYPRKSRATEFLFLILFIVLMIPISPLILVWGIGKII
                     EPVIELYNDVVWAPFNTLHNKINPYKEN"
     gene            7553..7816
                     /locus_tag="OO135_003373"
     CDS             7553..7816
                     /locus_tag="OO135_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399108.1"
                     /translation="MATLTKKEQAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            7827..7994
                     /locus_tag="OO135_003374"
     CDS             7827..7994
                     /locus_tag="OO135_003374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399109.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
BASE COUNT         2274 a         1689 c         2204 g         1933 t
ORIGIN      
        1 caaccttcag acgtattaac gtttggtaat aacgtttcca ttatcgcgtc ctcaacaatg
       61 aattttgtga tgcggtgcct ggtgcctcca ggtgacgtta accagttaac aattaacgcc
      121 ggatacagag aatccaccca taacactgtt tttggtttta actgttctgc gtgcgctcag
      181 ccgcattcac cacatcacaa aattcacttt aaaaagggcg gcagagcagt cacggagtaa
      241 aactgatacc gccaaacgtc accagaaaat tgataacaga gggcgttgca gcggggttgt
      301 cacttaagcg tatggtcaac ctgacaaccc ggtgtcctca gcgggggaag gaataacccc
      361 gccatactta ccgccgcgcc atttcgcgga ttgccacaac cggaagcgca cgatcgaatt
      421 aaatttaacg acgacctata cagagagact aacttctccg ggcgctttcg tgttatgccc
      481 tgacttttca gggatatatc cttttcagta aactgtcagt gccggattct tatccgtgtc
      541 cggcgcacgc actctacctc acctgtgaat aaattaatga ttaattgata ttttgttgtt
      601 tgattcaact ttcccatcgg atgtgtgatg ctttaaatca caggaattaa tactgcttgc
      661 tgtaaaatga ttttcaaggg gttctattcg aatccctttc tttttcatta acaagccaaa
      721 ccccttatca atgatgtcca ttagatccag gaagtatttt tcatgtaaat cctggttatc
      781 agagagctgc ttctcttcgt acagcccgat aaaggctcgg cgtacgttac cggatatatt
      841 gtcgatggtt tctttttcta cggtactcag gtcaagagtc gccagttgag agcgaaccac
      901 attcgatgcc atttcctgga atggtactgg taaatcttta aattccatcg tcaacctcat
      961 cagtcggagt ttcttgctaa ccagcgacgc gcgccagctt cggttttaaa cgttttgctt
     1021 ttggtatacg tcatggcggt gaatgtgccg tcctggttgg ggagcacacc acataccaga
     1081 gattcgctgt tgccaagatc gatagtatcc atgctgacct cattacccct taacgccggg
     1141 tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tgggtggtaa tttagttttc
     1201 tcatgaatgt tggtcaagca tttttaatga gaaaactcaa catttaatgc aaaataaagc
     1261 caatacattg aaatgtaagg ctttaaaatt tgtgaagggg gttactgatg tttgttacgt
     1321 ttgcgagctt ctagtagctc ggtgaatagg cgattaaaat tctcaacgcg ggcacggagt
     1381 tcgctgattt gtgcttgctg ctctgatttt ggaagtgcgc gatacaatcg caacatctcc
     1441 aactcatctt ccgataagtc taaggcgctg ttgagtgcta ctggtggatc tggtgtttta
     1501 tcctcgtcac caaacagtat ccaagttggt gaacattgca atacctcagc caggcgatgc
     1561 aaattttgcc cgcgcggggc tgtatggtcg ctttcccata gtgaaattga tgagccagat
     1621 acgccagcgg ctttgcttaa atcgttttga cttaaaccaa cctgtttgcg tctttctcta
     1681 attcgttgac ctaaagtttt ctcgttcata tttagatatc ttaataaccc ttgactcgag
     1741 attccttgaa tgattgctat tgagaaaact caactttgga ggggtgatgt ttaaatcaga
     1801 cgtaattaat ttttatggga cgaaagccaa agtagcgaaa gctgctggtg ttgatccatc
     1861 tgctgtttct caatgggggg aactggttcc tgaaggtcgc gcgatgcgcc tgcaagaggc
     1921 atccggcggg gaacttcagt acgaccccaa agtttatgac gaatatcgta aggcaaagcg
     1981 ggcggggcgg ttgaacaatg aaaatcaccc ctgaacaggt ttgtgaggct ctggatgcct
     2041 gggtatgtcg accaggaatg acacaggagc aagcgacgat attaatcacg gaagcattct
     2101 gggctctgaa agaacgcccg aacatcgatg ttcaacgcgt cacgtttaat gatggcgagg
     2161 ttgatcaacg ggcgctgggc gttaaccggg tgaagatatt cgaacgctgg aaagctatcg
     2221 acaccaggga taagcggaaa aaattcacgg cgctgattcc ggcaattatg gaggctatcc
     2281 gaattagtga tttcaggttg tatcgtgaga tcagtgatgg aaaaagcatt acgtacatga
     2341 tcgccggatt aaacaaagaa tatggcgatg tggtggagtc cgggctgctt tttgcggatc
     2401 cagctgttgt ggaacgtgag actgacgagc ttatagaaaa agctattgct ttcaagcatg
     2461 cgtatcgtca gcaatatcaa tattactttg cagataaaca aatgtctgcc aggggtttgt
     2521 atgagtatcg atgcactacg atgggctaaa aaggtgaaaa ccggcagttc atccagtaag
     2581 tctgtattga cctggcttgc tgatatgtgc ggtgccgatt tgtgtgcata cccgtctgta
     2641 tctgcactgg cagaagtaac ggaactaaac aaaaagactg tgcaggacag cttacgacac
     2701 ctgatggaga ttgggttaat tgttgatacc ggtgagagaa aaggcagaac aaagcaaatt
     2761 gtggtgtacc gacttatcgg tgtagaagaa agtgttgccg agcctgaata cacccaaaaa
     2821 cgggagtctt taaaggtggg taaaattggt gctgttaata aaaacagtac cgaaaatggt
     2881 tatgtttcag cacaaaacag acccaaaaac ggaactctta gctgcatgga aaataaccaa
     2941 agacacccaa attttccatc aaagacaccc aaaaacggat cacggaaccc aaaggaaccc
     3001 aaagatctaa accccacaca taacgcacgc gagagtgctc cgaccagtga gcaggaagtt
     3061 ttgtcgttac aggcagcacc ccttgtattc ctggatggcc tgagcgaacc catcggaaaa
     3121 tatccgatga ccgatagctg gtatccgtca cgggattttc gacgacgggc tgcgttgtgg
     3181 gggatggctt tgccggagac agaatttaca cctgctgaac ttgccgcctt ccgggactac
     3241 tgggcagcgg aggggaaagt gtttacgcag attcagtggg agcagaaatt cgcccgtcac
     3301 gtaaatcacg tcagggcgca ggttaaacca gtcagcaagg gggtaaacca tgcagcagca
     3361 ccaggtggca ccgcatcacg ggcagttcag gaaattcggg cagcacgtga gcagtgggaa
     3421 cgtgaaaacg gatttatcag cgatggaaac ggtctggaag ctgtgggaac tcatggggga
     3481 ggtttattcg aaccgctgga cccagaagaa cggggccgca ccttcgaagc tctggattgc
     3541 acagattggc gcgatgactg agcagcaaat ccgacaggtc tgccgccagt gcatggaccg
     3601 ctgccgggcg ggtgaaacat ggcctccgga cctggctgag tttgtggcgc tgatttcaga
     3661 aagcggggcc aatccatttg gcctgacggt ggatgctgtg atggaggagt accgccgctg
     3721 gcgtgatgag tcctggcgat atgacggaag cgacaaatat ccgtggcctc agcctgtgct
     3781 gtaccacatc tgcctcgaaa tgcgtaccag agggattgag cgccagatga cgcagggtga
     3841 gttaaaacga cttgcggaac ggcaactgac gaaatgggca aagcatgttg gtaacgggat
     3901 gagtgttccg ccagtgcgac gacaactgga aggggcgaaa cacccgcaag ggccaacgcc
     3961 aattgaacgg ctgaaacagg aatacgaacg ccggaaggca gctggtttta tttgaatctg
     4021 agaaacgatt ttgtcggagg aaattttaat ggaaaccgta tttgacgcac tgaaagcaat
     4081 gggaaaagcc acatcggtag aactggccgc gcgacttgat atcagtcgtg aagaggttct
     4141 caacgagctg tgggaactca aaagaaatgg cgtcgttgat aaaactggtc acacctggtt
     4201 tctggctggc gaaggtgaat cccgggtaac cgaagagcgg ccagtaaaat ctgaagcaca
     4261 ggatatgctg accggggagg tcgaacaaaa agttaccgca gacatgatga ttgagtttat
     4321 cggtcaggat ggtgctaaaa cgtgtgagga actggcgggt aagttcggcg tcagtactcg
     4381 caaggttgcc tccacgctgg cggtggtaac cgcaacgggg cggctggcac gcgttaatca
     4441 gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcagagc cgaaagccgc
     4501 gctggtaacg gaaagtgatg gtaaggcctt tcctcagcca gcaggtgctg cgttaccagt
     4561 ccgggaagcc gcaacacagg aagaaattaa aacagaaact gtggcggaca ttgtgcagcc
     4621 gttgccatcg tttaccgaaa cgcaagcaga tgagctgatt tttccgtccc ttcgcagggc
     4681 aaacctggcg ctgcgcaggg cgaaaagtga tgttcagaag tgggagcgag tctgcgccgc
     4741 gctgcgggag ctgaacaagc accgggatat tgttcgacag attactgatt cttcccgccg
     4801 tgttgtatcg gaaaagtgat tgccggaggc gcttatggca aaagtattta caccagaaga
     4861 gcgggaagaa gtgaaggcgc gcattgtgga attcgtgcgc ctgagcggac gagaaacttt
     4921 tcgacaactg gcagataaaa cgggtgtcag taagaccgct attcgtcgtt tatctggtgc
     4981 gcttgcggcc agtggtgatg tctggctctc tggttgcggg gtatttccat cagagcaggc
     5041 gtatcgcgta tggcgtaaga caccggagaa ggctgctgac ccgacactga ttcgaaagtt
     5101 acctgacgga gaaatacgtc gttacaacag acggcagaac ataatttgtc gtgagtgccg
     5161 ccagagcgaa gttatgcagc gtgtgctggc gttctatcgg ggaaactttc aggaggtgat
     5221 ggagtgaggg tcagagttta tattgccggt ccaatgacgg gatatgaaaa tttcaaccgt
     5281 gaggcgtttc acaaggcgga agaggaactg aaacgggaag ggcataccgt cttaaacccg
     5341 gcagtacttc cggacgggct gacacagccg cactacatgg atatttgcat ggcaatgatt
     5401 cgttgtgtgg atgcgattta catgctgaat ggctggcagc ggtcagcggg cgctaaggca
     5461 gagctggcac tggcggagaa actggggcat gcagtgattt atcaggaggt ggctcaatga
     5521 gagaggttaa ctatgaggcg cttcgtgagg cagcacaaaa ctatcagtcg acgctggcgt
     5581 ggtatcaggc tatcccggac agcccaaatg ctgaacggga ttgtgatgcg gctcttgctg
     5641 cgtttaagcg tcacatccgt catcgggaag cggatattat cgctgatttg ctggatggac
     5701 tggaagaagc aaaatcacaa ctcaaagagc agcgtgagta ttacgaaggc gttatctctg
     5761 atgggagcaa gcgtattgct gaactggaag cgcgggaagt tcaattaccg actcgctacg
     5821 accttcgata tggacacccg ataaatgcag atgagcgaca tgtcatgata cctaaagaaa
     5881 atggcagttg gctttacctg attgacctag aacacgcatt acgcgtcgct ggcattcgca
     5941 tcaaaggaga ggagcatgga aataaaacca gaggatgagt taagcaatat cgttttattt
     6001 ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc atcggaaaga
     6061 ccatattgtc atcacgcctc tgttcgggtt gacgaaaaag agcgtcaggt ccgctgtaaa
     6121 atctgcggtg cagttgtgga gccatttgac tggatgctct ctgtggcgaa aagagaaacc
     6181 agactggcag atgatgtaag gctattgcgc caggaggaac aggaaaggcg gaaaaatata
     6241 gaaaagttaa ttcagattga gcgtaacgcg aaagcgcgga tatgcagggc gacaaaatcc
     6301 agaactgaat aattaaattt agcactgtaa ataaaatcaa atccttaacc ggagggattt
     6361 ctgcaccctc agaacatcag gaggccgtcc gaaagggcgg tagtgaaatg cgaaaattca
     6421 aaataattat tgaaacggga atagccggtg gagatttcga ggatgaattc gaagtggatg
     6481 atgatgcaac accagatgaa atacaggatg aagctaaaga tattttcttt aactactgca
     6541 attactcata ccacgaaata aaagacgaag aggaagaaca aaatggctga ttttggttca
     6601 actaaatata acgccagttt tgaagaatgg catgaactgt taatggatta tgcagagtta
     6661 cgcggtggaa gtgccgctga tgctgaagca tggcgtgatg attatgaagc agggaaaaca
     6721 ccggtcgaag catattgtga tgagtggggc gatgaatgag cgagattaat tatcaggaag
     6781 ggcatgaaaa ggcagggcag gcaaaaccag tggcatggcg atatcgctac gtgaaaaaag
     6841 gcgttacaga ctttcagggg aagcagtggg ttggtgactg gaaatatgtc ccgacaaaag
     6901 aggattgtaa cgacagacca aactatgaaa ttcaggcgtt attcacggcc ccgccagctt
     6961 cggtgacatc agaaggactg gttaaagctg tgcgctttta tgaacaggta cggcgtgagg
     7021 atccgccagt tgaaaccgga gcatggaaag acgctattga ctgggtactc aaggaggcct
     7081 gcctggttgt aaacacgggc atcaaaggag gctgaatggg tattgccgca agttatacca
     7141 tgcatctcta ttgtgattgc cgccagtgta cggatggcaa atatcagtcg ccagattttg
     7201 gtgagtatat gggtacgtca tgggctggct gtgcaaaaga ggcacgtaaa gatgggtggc
     7261 gaataagtgc cgacaaaacg cgtgcttttg cgccagggca taaaattttg aggagcaaca
     7321 aaggagagtg atgtgcctac attattcaga aaagaatatc cgcgaaaaag tagagcgaca
     7381 gaatttttgt ttctcattct gtttatcgtg ttgatgatac cgatatcccc gttaattctg
     7441 gtatggggaa tcggaaaaat aattgagcca gttattgaat tgtataacga cgtggtatgg
     7501 gctccgttca acacactgca caataaaatt aatccgtata aggaaaactg atatggcaac
     7561 tttgacaaaa aaagaacagg catggttgaa cgaattacag gacgttcttg atcgctgtcc
     7621 atcaccgaaa aaaattggtt tttacaccat tggcgataaa agcatttacc tgtatgacct
     7681 gcgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcaatgg attggtgtgt
     7741 tgctgtccat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
     7801 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
     7861 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
     7921 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
     7981 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
     8041 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
//
LOCUS       DAJTMK010000166         7993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTMK010000166 DAJTMK010000000
VERSION     DAJTMK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.170"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..190
                     /locus_tag="OO135_003375"
     CDS             <1..190
                     /locus_tag="OO135_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399110.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO135_003376"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO135_003376"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5399111.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO135_003377"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO135_003377"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5399112.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO135_003378"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO135_003378"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5399113.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO135_003379"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO135_003379"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5399114.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO135_003380"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO135_003380"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5399115.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO135_003381"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO135_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5399116.1"
                     /translation="MAFLTSSDKALWHLALPMIFFNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO135_003382"
     CDS             7144..7353
                     /locus_tag="OO135_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5399117.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO135_003383"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO135_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5399118.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKSR"
BASE COUNT         1809 a         1965 c         2195 g         2024 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattcc acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tccccagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttactagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcaa
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttgttcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tcttcaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcgct ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gactgttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cgatttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gta
//
LOCUS       DAJTMK010000167         7925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTMK010000167 DAJTMK010000000
VERSION     DAJTMK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.171"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(132..863)
                     /gene="dnaQ"
                     /locus_tag="OO135_003384"
     CDS             complement(132..863)
                     /gene="dnaQ"
                     /locus_tag="OO135_003384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5399119.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            928..1395
                     /gene="rnhA"
                     /locus_tag="OO135_003385"
     CDS             928..1395
                     /gene="rnhA"
                     /locus_tag="OO135_003385"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5399120.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1392..2114)
                     /locus_tag="OO135_003386"
     CDS             complement(1392..2114)
                     /locus_tag="OO135_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5399121.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2148..2903
                     /gene="gloB"
                     /locus_tag="OO135_003387"
     CDS             2148..2903
                     /gene="gloB"
                     /locus_tag="OO135_003387"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5399122.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2975..4333
                     /gene="mltD"
                     /locus_tag="OO135_003388"
     CDS             2975..4333
                     /gene="mltD"
                     /locus_tag="OO135_003388"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5399123.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4382..4978)
                     /locus_tag="OO135_003389"
                     /pseudo
     CDS             complement(4382..4978)
                     /locus_tag="OO135_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5009..5809)
                     /locus_tag="OO135_003390"
     CDS             complement(5009..5809)
                     /locus_tag="OO135_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5399124.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6050..6964
                     /locus_tag="OO135_003391"
     CDS             6050..6964
                     /locus_tag="OO135_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5399125.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(6961..7764)
                     /gene="dkgB"
                     /locus_tag="OO135_003392"
                     /pseudo
     CDS             complement(6961..7764)
                     /gene="dkgB"
                     /locus_tag="OO135_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2112 a         1903 c         1927 g         1983 t
ORIGIN      
        1 cgttgtgttc cgttgggcac gaggcgaata ttacggattg cctcaccctt cgtcaacggt
       61 ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca agccgctatt tttagcgcct
      121 tttacaggta tttatgcccg ccagaggcag cttccgccct tcttctccac cagatcaaga
      181 cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc
      241 tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc tccttccatc
      301 gcaaaagcca tcgacgtttg accaccggtc atcgccagat aaacttccgc aaggatctgt
      361 gcatcgagta atgccccgtg tagcgttcgt ttactgttat ctatttcgta gcgggcacat
      421 aacgcatcga ggctgttgcg cttaccagga aacattttcc tcgccaccgc aaggctatcg
      481 gtgaccttac agaaggtatt ggtcttcgga atatcgcgct taagcaacga aaactcgtag
      541 tccataaagc cgatatcgaa cgctgcgtta tggatcacca actccgcgcc gcggatatag
      601 tccatgaact catcggctac ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca
      661 ataccatgta cgccaaaggc ttccggatcc accagccgat cgggtttgag ataaacatgg
      721 aagttattgc ccgtcaggcg acggttcacc acttcaacgg caccaatctc aatgatcttg
      781 tggccttcat agtgcgcacc aatctggttc ataccggtgg tttcggtatc gagaacgatc
      841 tggcgtgtaa ttgcagtgct catagcggtc atttatgtca gacttatcgt tttacagttc
      901 gattcaatta caggaagtct accagagatg cttaaacagg tagaaatttt caccgatggt
      961 tcgtgtctgg gcaatccagg acctgggggt tacggcgcta ttttacgcta tcgcggacgc
     1021 gagaaaacct ttagcgcagg ctacacccgc accaccaaca accgtatgga gttgatggcc
     1081 gctattgtcg cgctggaggc gttaaaagaa cattgcgaag tcattttgag taccgacagc
     1141 cagtatgtcc gccagggtat cacccagtgg atccataact ggaaaaaacg tggctggaaa
     1201 accgcagaca aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga tgctgcccta
     1261 gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa
     1321 cgctgtgatg aactggctcg tgctgcggca atgaatccca cactggaaga tacgggctac
     1381 caagtcgaag tttaagcttg tggtttacga cattgccggg tggctccaac cgcttggcga
     1441 attcgtggct tgtttttact ctgtttcatc gggtttagcg ttaaaggaat agtccgtttc
     1501 cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa tagtttacct
     1561 ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt gcagcacttc aaaattcaac
     1621 aaagagagcc agtccagttg ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt
     1681 tttcgcaata ccggcacaag tttgcgtaat cccattaaac taatgggatt gaagccacta
     1741 atcaccagcc agccatcatc aatcaatacc cgatcggttt cacgcaataa acgatgcgga
     1801 tcggtgcacc acggcaatgt atgtgccagt agacaaacat caacggattt atcggcaaaa
     1861 ggaagatgaa gtggatccgc ctgcacctgg acgggcattc cttgcgcaga aacattcact
     1921 tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc
     1981 agatgaaaac catacatttt agtgaaccac gggttgagct ggcgctccag cgccttgcga
     2041 taaagctctc cccagggcaa atcgccccag cgatcaggag ccacgacagt ttgagggact
     2101 cttgccggtt tcatcacaac cttccgtttc acactaagag gtaatctatg aatcttaaca
     2161 gtattcccgc ctttgatgac aattacatct gggttttgaa tgatgaagca ggtcgctgcc
     2221 tgattgtcga tcccggagac gcagagccag tattaaacgc cattgccgcc aataactggc
     2281 aaccggaggc catatttctc acccaccatc atcacgatca cgttggcggc gtaaaagaac
     2341 tggtggaaaa gtttccgcaa attgtggtgt atggtccaca agagacacaa gataagggaa
     2401 caacacaggt agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa
     2461 ttgccacacc gggtcacact ttaggacata tctgttactt cagtaaacct tatctatttt
     2521 gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa
     2581 tgtatcaatc acttaataag ttaagtgcgt tacctgacga tacattggta tgttgtgctc
     2641 atgaatatac gttatcaaat atgaagtttg ctttgagtat tcttccgcac gatttgtcca
     2701 taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata acactacccg
     2761 taattctgaa aaatgagcgg caaattaatg tttttttaag aacggaagat attgatttaa
     2821 ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt gcatggttaa
     2881 ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt cgccgttatg
     2941 atcggtcgtc ttttaagcaa ctattgacac acacatgaag gcaaaagcga tattactcgc
     3001 ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg cacagagcct
     3061 ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat ggatggacga
     3121 tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg agctaaagat
     3181 gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac gcaataagag
     3241 ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag cagggcaagt
     3301 taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga gcgcttttga
     3361 tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc cgagcacggg
     3421 gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc
     3481 aacaactgcc gcgctgaaca tgatgcagcg tctgaacaag atgtttgacg gcgactggct
     3541 tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa
     3601 caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg aaacgaagca
     3661 gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc gttatggcgt
     3721 acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga gcagcccggt
     3781 tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga agacattcaa
     3841 cgctggcgta aaaggctcca cgctgggcgc aagtggcccg cagtacgtga tggtgccaaa
     3901 gaagcatgca gatcaactgc gtgattctct cgcttcaggc gaaattgctg ctgtacagtc
     3961 gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac gctctggcga
     4021 cacgctttca agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa
     4081 caaactgcgc ggatctaagc tgaagccagg ccaaagtttg acgattggtg caggcagtag
     4141 cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca aaggcgattc
     4201 gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc gctggaacag
     4261 cgacactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa acaacaacat
     4321 gccagattcc tgacaaacca gataataaaa aaggcaccga ttcccccggt gcctttttta
     4381 tttatgccgc tttatgtgct tctaccatga tgatatcact ggtgaaagag ccatcagtct
     4441 gcaaggaaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt
     4501 ctactaacgc ttctggcgta cgcattctca cgacccatga agaaaattcc agcggtaact
     4561 tatctgtaat taaattatca actatcagat tggcttcatt gattaacgtc aaccacgcac
     4621 cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc tgtaaccaga
     4681 tgtcgcgcac tgggtgaccc ggagacatta catccatcac aatcagccta ccgccaggtt
     4741 tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga tgggcagaat
     4801 aacggctgat aacaatatca aatgcgttat cggcaaatgg cagacttgcg gcatatccct
     4861 ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccacg acatccagca
     4921 tgtgggccga taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg ccaaacgttg
     4981 gtctgccctg tggcagacct gacatacctt atttatcagg cttgccggga ctgaattcaa
     5041 cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttca ctgacgttca
     5101 gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg
     5161 taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg ttcatccttc
     5221 tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg tgagctatct
     5281 gatcaccaat aggaagtaac tgcttactat agacatccac gcccagactg aagttgacgg
     5341 cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg
     5401 ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa tacactggat
     5461 gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa gcgggtacct
     5521 gatcggcggc aagatagtta gcggtcgcaa actgtactaa ctctggcgtt gtctgcgctt
     5581 cctgcaataa caccagatgt gcatctttgc cgtagttctt taataccgac aaccattcag
     5641 cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg gtacttaacg
     5701 gttccccagg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt aagatccttt
     5761 ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact tttgactttg
     5821 aaacctctat tacgtcaacc cttccgcact cgggagggct ctactattat agggatttta
     5881 gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta caccggtcag
     5941 aattttgctc attatggaga acattaaatc gcatgacata tcacgcatcc tgatggttta
     6001 atgacaaata aattcaaaat atctcttcta ttttgcaaca ggagcaaaaa tgaaagccac
     6061 gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc
     6121 ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc tggagatgaa
     6181 acttggcgtt agcctgctta atcggaccac acgacaactt agcctgacgg aagaaggcga
     6241 gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag aatcagaaat
     6301 tatggagacg cgtaatacac cgcgtggact gttacggatc gatgccgcaa ctccagtggt
     6361 gctgcacttt ctgatgccgt taattaagcc tttccgtgaa cgctatccgg aagtcacttt
     6421 gtcgctagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat
     6481 acgcgctggt acgttaacgg attccagctt acgtgccagg ccgttattta acagttatcg
     6541 aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa cgatcgacga
     6601 tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata cctggccgat
     6661 agcctgtagt gatggacaat tacatgaggt gaaatacggt ttgtcatcca atagtgggga
     6721 aacactgaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg actacatgat
     6781 cgacagagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag tgttgccagt
     6841 ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc gcatccgggc
     6901 ttttatcgat ttccttagcg ggcatgtaaa aacagctccc ggaggagctg tcagagaggc
     6961 ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc aatccagtgc
     7021 ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa gattactttc
     7081 caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca tagcccacgc
     7141 cagaatcact tgtgccggag tcgcattgtg tttagctgcg atacgagcaa taacctcatc
     7201 tttcagggcc ttaccatacg ccagcgtcat ataggaagta atatggatgc cgtgctgttt
     7261 agcccaggca accactttac ggttttgcag ataaggagag agttcaatct ggttagtagc
     7321 gatgttttca gcgccaacag cagcaatcgc cttttccatc aatgggatcg tgaagttgga
     7381 aataccgatc tcacgcgtca gcccttattt tttggcttcc agcagcgcct gcataaactc
     7441 ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat caacataatc
     7501 ggtacgcaat ttttgcaggc tctctttcag acttgggatc aatttgtctt tgctgagatt
     7561 ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac tttctgcaat
     7621 cgcctgacct actgcggctt cgttatcata gatttgtgcg gtatcaattg cgcgataacc
     7681 aagttcaagc gccgttttca cagatgaaat aacaacgtcg tctttcagac ggaaagtacc
     7741 taaaccaaat gcagggatag ccataatatg cctcttttaa tgctattttc gttaactgag
     7801 gtgattatgg gcttcagact gttgaggaaa aaaaagcgta aaatgcagag gatttttgcg
     7861 attctggcaa taatagatat acagacgaaa aaaaacctcg agcgttaact cgaggcttct
     7921 taata
//
LOCUS       DAJTMK010000168         7848 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTMK010000168 DAJTMK010000000
VERSION     DAJTMK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.172"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(125..4027)
                     /gene="hrpA"
                     /locus_tag="OO135_003393"
     CDS             complement(125..4027)
                     /gene="hrpA"
                     /locus_tag="OO135_003393"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5399126.1"
                     /translation="MTEQQKWTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAFDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            4228..4833
                     /gene="azoR"
                     /locus_tag="OO135_003394"
     CDS             4228..4833
                     /gene="azoR"
                     /locus_tag="OO135_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5399127.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIACGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(4884..6200)
                     /locus_tag="OO135_003395"
     CDS             complement(4884..6200)
                     /locus_tag="OO135_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR5399128.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHLRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(6190..7848)
                     /locus_tag="OO135_003396"
                     /pseudo
     CDS             complement(6190..7848)
                     /locus_tag="OO135_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         1927 a         2176 c         1875 g         1870 t
ORIGIN      
        1 ctaaatcagt aagttggcag cattacctga attcctgaat ttttaccctt accgttttag
       61 aattaataaa aaacccggcg ctaaatgctt accgggtttt agatttatta ggcaaatagc
      121 agggttaacc gctaatctgc tccatcgcct gcaaaatacg cttatctgaa atcggataag
      181 gcgtaccaag ttgttgagcg aagtaactaa cgcgcagctc ttctatcatc caacggatct
      241 ctttcacgtc ttcatcctca cgacgtgcag gcggcagttt gttgatccat tgctgccacg
      301 cctgctggac gttttcgact ttcagcatct gtgcacgatc gcgatgtgga tcaaacgcca
      361 gtttttccag tcgtttttca atcgcctgca aatatcgcag cgtgtcgccc agccgtttga
      421 agccgttacc agtgacaaaa ccgcgatata ccaacccgcc catctgcgct ttaatgtcag
      481 aaagccccag tgccatggtc atatccaccc gccctttcag acgtttgttg atattgaaca
      541 ctgccgtaag aatttgctcg acctgcttcg caatatctac caccgtgtcg ttcagttcgg
      601 cacgcacttt ctcatgcagc gcagcaaagc cttcttccgt ccagaccggg ccaccattgg
      661 cgtcgatcag ttgatccaca ccgcaggaga tacagtcgtc gatcagctcc agcactttgc
      721 catacgggtt gaagtacagt cccagcttgg ctttgttcgg taacttttca tgtaaatact
      781 tgatcggcga gggaatattt agcagcagta gacggcgaag accattccac attgcctgtt
      841 tttgctccag cgggttatca aacagtttga tcgccacgct gtcgcgttca tccaccagcg
      901 ccggccacgc cttcacttta tagttgccac gcttctgttc gtaactttcc ggcagctgac
      961 caaaactcca gatatgtaag ccgctctgct cgataccgtc atccgccacc gcggacagcg
     1021 tttcctgcac tttgcctttc agcgcatctt tcagatcttg tagcgagcgc ccttctttta
     1081 gcttcttgtt tttgtcatcc accacgcgga aggtgatttt caggtgatcg ggcacctgat
     1141 cccagtgcca gtcttcgcgg tcaacggtaa cgccggtcat ccgccgtaac tcgcgctcaa
     1201 ggctgtcgag caacggtaac tccagcggtg tgacgcggcc taaaaacgct tcggcgtagt
     1261 ttggtgcagg cacaaaatta cggcgtaccg gtttcggcaa cgatttaatc agagcaatca
     1321 ccaattcgcg gcgcagaccg gggatctgcc attcaaaccc gctttcctca acctggttaa
     1381 gtaacggcag cggaatatgt acggtcacac cgtccgcgtc tgcgcctggc tcaaactgat
     1441 agctcaaacg cagcttgaga ttgccctgat gccagaagtt cgggtaatcc agcttgctga
     1501 ttttttccgc cccttctttg atcaacatgc tcttttcaaa gttgagcaaa tcaggcgttt
     1561 cgcggctgac ttttttccac cagctatcga agtgacgagc ggagattaca tcgtggctga
     1621 tgcgctggtc gtagaactca aacagcgttt cgtcatcaac cagaatatcg cggcgacgtg
     1681 atttgtgttc cagctcttcc acttcggccc gcagtttcag gttttcacgg aagaatgcgt
     1741 gacgcgtctg ccagtcacct tccaccagcg catggcgaat aaagagttca cgacataacg
     1801 ccggatcgat ctggctgtag ttgaccttgc gcgcggcaac aatcggcaaa ccataaacag
     1861 tgactttttc cgttgccatc accgcgccct gcgcccgttc ccagtgcggt tcgctgtagg
     1921 tacgtttaat caaatgctga gcgacaggct ccacccattc cgggtcgata cgcgcagcaa
     1981 tgcgccccca caggcggctg gtttctacca gttccgccac cattacccat ttcggcggtt
     2041 ttttgaataa accagaaccg gggaagatgg agaaacgcgc gttacgtgcg ccggtatatt
     2101 cttgtttatc ggcatctttc atgccgatat gggaaagtaa acccgtcagc aacgcaatgt
     2161 gaatttcgcg atactccgcc ggttcgctgt taaccggaat gccaagttct ttcaccacct
     2221 gacgcaactg ggtgtagata tcctgccatt cgcgcacgcg cagatagttg agataatcgg
     2281 tacgacacag gcgacggaag gcgtttgaag aaagcgcctt ttgctgctcg ccaagataat
     2341 tccacagatt tacaaacgcg aggaagtcgg attctttgtc gtggaagcga cgatgttttt
     2401 cgtccgatgc ctgctgtttg tccatcggcc gctcacgcgg gtcctgaatg gagagcgcgg
     2461 acgtgataat catcgcctca cgcacgcagc catgtttctg cgcttccagc accatacgcg
     2521 ccagacgagg atcgaccggc agttgcgaga gctggcgacc gagcggcgtc agtttatagg
     2581 cgctggcctg ttcatccgtg gtgatcgcgc ccagttcttc gagcagacgc acgccatcct
     2641 ggatattgcg tttatccggt gcttcgacaa acgggaacgc agcgatatcg cccagcccca
     2701 gcgcggtcat ctgcaaaata accgaggcca ggttggtacg cagaatctcc ggatcggtaa
     2761 actccgggcg cgagaggaaa tcgtcttcgg aatagagacg aatacagatc ccttcggaca
     2821 cacgaccaca acggccttta cgctgattgg cagacgcctg tgaaatgggt tcaatcggca
     2881 aacgctgcac tttggtgcga tagctgtagc ggctgatacg cgccgtaccg gggtcgataa
     2941 cgtatttaat ccccggtaca gtcagcgagg tttccgcgac gttggtcgcc agcacaatgc
     3001 gtcgcccgct gtgcgactgg aacacgcggt tctgttcgct attcgaaagc cgcgcataaa
     3061 gcggcaagat ttcggtatgg cgtaagttca gcttgttcag cgcatcggcg gtatcgcgaa
     3121 tttcccgctc gccgctcata aagatcagaa tgtcgccagg gctttcctgg ctcagttcgt
     3181 ctacggcgtc aaaaatcgcc tgcaactgat cgcgctcggt gtcatcggct tcttcaacaa
     3241 tcgggcgata gcgcacttcc accggatagg tccgaccgga gacttcgata atcggcgcat
     3301 tattaaagtg gcgagaaaag cgttccgggt cgatagtcgc ggaagtgata atgattttta
     3361 ggtcaggacg ccgcggcagc aactctttca aatagccgag cagaaaatcg atattcaggc
     3421 tgcgttcgtg tgcttcgtca ataatgatgg tgtcgtactg catcagcagg cggtcttgct
     3481 ggatctccgc cagcaggata ccgtcggtca tcagcttgac catcgtgtta tcacttacgt
     3541 gatcgctaaa acgcactttg taaccgatgc aaccgcctgg ctccgttttc agctcttccg
     3601 caatacggtt cgccaccgtt cttgccgcca gacgacgcgg ctgggtatgg ccgatcagtc
     3661 ctttaatccc gcgccccagc tccatacaga ttttcggtag ctgggtcgtt ttaccggaac
     3721 cggtttcccc ggcgacgatc accacctggt gatcacgaat cgcttcgaga atatcctgtt
     3781 ttttctgact aactggcaaa ttgtccggat aagtgatttc cggtcgtgcc gcttcacgca
     3841 gcaggacttt ccctgccgcc tggtcaatct ctttcgccat ctcctggaaa atggcctgtt
     3901 gtgcatcagg atttttaacc ttcttaacac catgcagacg gcgagaaaaa cgcagtctgt
     3961 cgcgcagcat caacgaatcc agccgctgct gcaaagcagt aaaggtccat ttttgttgtt
     4021 ctgtcataac gttagtgggc agtagtctgc ctgattaatt ttcttttaaa tgatagatat
     4081 agagtaccac atcgcggctt tctatgcgtt gttcaataaa tccgaacata ggcttcgatt
     4141 tattgcgcta tctctgtggc aggattgtga ataaagtgtc agcaagcaac ggggcatcgc
     4201 caattcaaac atctataagg aaacaccatg agcaaggtat tagttcttaa atccagcatc
     4261 ctggcagggt actctcagtc taatcagttg tccgattatt ttgttgaaca atggcgcgaa
     4321 aagcactccg ctgatgaaat caccgttcgc gacctggctg caaatccgat tccggtactg
     4381 gatggcgaac tggttggcgc tctgcgtccg agcgatgcgc cgctgactcc gcgtcagcag
     4441 gaagctctgg cgctttccga tgagctgatt gccgagctga aagcccacga cgttatcgtt
     4501 attgcggcac cgatgtataa cttcaacatc tcaactcagt tgaaaaacta ttttgacctg
     4561 gttgcacgcg caggcgttac tttccgctat accgagaacg gtccggaagg tctggtaacg
     4621 ggtaaaaaag ccatcgttat taccagccgc ggtgggatcc acaaagatgg accaacggac
     4681 ctggtgacgc cgtatctgtc cacgttcctc ggctttatcg gcattaccga tgtgaaattt
     4741 gtcttcgccg aagggatcgc atgcggtccg gaaatggcag cgaaagcgca gtctgacgcg
     4801 aaagcggcca tcgacagcat tgttgctgca taaccggaca cgatcttgat gtggactgta
     4861 gttcagtcca catctcaatc cacttacctg ttttcccata atttcagcat cgctttgtga
     4921 tcttccttaa gcacaatatg cgaacgtctg gctcgaatat aactaatcgc ttcatcaacg
     4981 gttttacagt gtccgtaaca taacaaccat gccgccacta ccagcgcact gcgcgataat
     5041 cccaacgcgc aatggaccag aacgctgcct tgctcttcgc gtaatgtttc cagcatcgcc
     5101 acggcctgtc ggagctcccc ctcttccgga accaccagat ccagcatcgg tacacagaaa
     5161 tagagtcgat cttttgtcgc tcgtccccga gggaattcaa aggtgacgtc cagaaccgca
     5221 ttctgtgccg gaatatgtcg tggaaacgcc cctaaataaa caccagcagt aattttgctc
     5281 accggctcca ggcgacgaca aaaccaacgc atagacagcc acatcccgat gcgccagggc
     5341 aatgtcagcc agtaaacggc gggcgggagt ttcccctgac tatctttccc tgttgttatc
     5401 gcgccaagcc caccgtaccc acggccaata atgagtagcg ataatactgg ccaacataac
     5461 cagactgacc accataactg aatcatcacc attagctcca tcaacacaat gcacgagccc
     5521 gcgcctacga catatggcag tgctattttg attcgacgtt gatcaggttt ctgataattc
     5581 caacgacgat cgacgggcac catccagtca atcaacatac ctaccgccag ccctgtgatg
     5641 acatcaataa aatgatgctg ccaggtcgtc agagtagaaa tagcgatgag taaaaaccat
     5701 ccgccacaga ctttacgcca cctctcagcc agatgctgac gcaagtgacg ccaaagtagc
     5761 cagcagagaa taatatgcag cgaaggagac tggttataag gcagatcaaa cagttcaagt
     5821 tgcgaaaata gccatcccgt caccccactc acttcaggac ggataaaact aaacttcagc
     5881 ggatagagca aaaaaccgca gcaggccatt accgttgcta gaataagccg gtggacaagt
     5941 cggcgctgtt cgaatgtcgt gctacaaacg aacagcgaaa atccatataa aagatccaga
     6001 ctccagtaag gaacaatagt ccagggaaga aaagggatcg ccgtttccca accgaatacc
     6061 tgactgggga tatcatggct gttaaggtcc tgaaccgcgg tgaactgatt aagagatcca
     6121 taggtgaaga aaaaaaacgg ggccagcaac aataaccagc cagcgccttg tagcaatacg
     6181 ttccgacgtt cagttatcac gacgcaccgc catcgaaacc gtaaaaatac cccactcatc
     6241 aatccgttgt gtgcatttat caaatccggc atcacgcacg agtgaatcca tctccccttg
     6301 cgaacgcacg cgcattaccc acggtttacc atctttatga ctggttaaca ccccggcaat
     6361 catctccagt tgagggtgcc acggctgccc ggtgtagatg agaatgccga ccggttcgat
     6421 ggcattggca agacctgcga gtgagttttt tacctgctca ttttcgggaa aaagctcata
     6481 cagtccagag acaattgcca gcgtaggccg cggtgttaac gcgctgagtt cctccgggtt
     6541 aaacgcatcg ccctgttcaa aacgcacccg cccagacatt ccccgttggg caatcatctc
     6601 ttgcccctgt gcaacattta actcgctgta atcacgtaac aaaatatcgc ttacggcagg
     6661 ctcgtttgcc agcgcatcca gtacatagcg cccatgccct gcggcaatgt caaccacgcg
     6721 gacggctaaa cctttggcgt ggagatcggc aacggcttgt ttaatcagta tttgtaaatg
     6781 ggttttgcgc tggcgaatac cgcgccagcc aacactgttc aggtagattt tgtcgattaa
     6841 gcgcccgaat gcgttactac cttgcggttg attttgatag acataatcca gcgaactgcc
     6901 tgaatcaaag ccggtgctca ttccgagatg caggcccgca gagtgcgtcc cgaacaattt
     6961 catcgccttt cgcataaagc gatacgctaa atcaaccggc gataatggca cgggtccacc
     7021 agaaagtagc cgccattgat ccgctgatgg tcctgtgcag tcttcatgct gataatcaaa
     7081 tttttgcgat ttgttagcat ataaacgact aataaagctt tgcatttttt caaatgccag
     7141 cgccctgttc tcttcaccct acgtgtcgtg ataaaagcct ggcagcagat gcagctcttt
     7201 cagagggcta cgtaatctct gataaaaatc aatctgcggt tggcgatgca ccacatagtc
     7261 atcgcctgat atcagaagtt gcgtggggag cgtaatcgcc gccgcatctc taataatacg
     7321 ttcagacgtt ttatagagat cgagcaagat attgacggca atcgcccgtg tgatcagcgg
     7381 atcattattg aaactcgcca cccgttgccg atcgtgggtc aaatagcgtc ctttcacata
     7441 ggaattaata aaaaacagac cacgcagacg atgccataac gccagcgcag gacgtgccag
     7501 cggcacatac aatttaacct taaaggccgg agaagccagc accagcccgc gaattgcagg
     7561 cgcataatca tgaacctatg tggcaaccag cactgcgccg acgctttgcg cgatcacaac
     7621 cacctcttcc agtccaacct agctgtcgct ggcagcaaaa cggacaaatt catcgacatc
     7681 ctgcactgaa cgcgcaagat atgggctata accacgcggc cccgaagttt ttccatgccc
     7741 ccgggcatcc catgcataaa aagcagtatc tggcatcgcc agttcatcaa cgatatgttg
     7801 tagacgacca gaatgttcat ggccacgatg aaataagaca atgacctt
//
LOCUS       DAJTMK010000169         7832 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTMK010000169 DAJTMK010000000
VERSION     DAJTMK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.173"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            608..1993
                     /gene="dcuC"
                     /locus_tag="OO135_003397"
     CDS             608..1993
                     /gene="dcuC"
                     /locus_tag="OO135_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5399129.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTILAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(2034..2714)
                     /gene="dpiA"
                     /locus_tag="OO135_003398"
                     /gene_synonym="citB"
     CDS             complement(2034..2714)
                     /gene="dpiA"
                     /locus_tag="OO135_003398"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR5399130.1"
                     /translation="MTAPLTLLIVEDKTPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2683..4341)
                     /gene="dpiB"
                     /locus_tag="OO135_003399"
     CDS             complement(2683..4341)
                     /gene="dpiB"
                     /locus_tag="OO135_003399"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR5399131.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRMATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPLGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4744..5802
                     /gene="citC"
                     /locus_tag="OO135_003400"
     CDS             4744..5802
                     /gene="citC"
                     /locus_tag="OO135_003400"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5399132.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSMHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5839..6570
                     /gene="rna"
                     /locus_tag="OO135_003401"
                     /pseudo
     CDS             5839..6570
                     /gene="rna"
                     /locus_tag="OO135_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
     gene            6800..7210
                     /gene="rnk"
                     /locus_tag="OO135_003402"
     CDS             6800..7210
                     /gene="rnk"
                     /locus_tag="OO135_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5399133.1"
                     /translation="MSRPTIIINDLDAERIDILLEHPAYAGLPIADSLNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(7441..>7832)
                     /locus_tag="OO135_003403"
     CDS             complement(7441..>7832)
                     /locus_tag="OO135_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399134.1"
                     /translation="EGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGL
                     TFKMGQTHVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVI
                     LVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2015 a         1997 c         1745 g         2075 t
ORIGIN      
        1 catatttact cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat
       61 agcaaagcta caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct
      121 gctctattta tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc
      181 gtcgttttct gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa
      241 tgcaatcaag gaaaaattat tttatttttt aacgagatac aacaatcatc ttaacgaagt
      301 atataatata tatcgtcaac ctatgctcat gttagattat tttttgagcg cacagccgca
      361 gctaacacca ttgcaattaa caaatttgca tcaatccacc atcaatttgc acacattatt
      421 atgtgataat tgccaaccgc taaatatgcg ttttgttatc tatgtataaa aacagcaact
      481 tcaatgtctt aatggcagtt tttcttgatt ttaatcagca ttcatcgcca atttattggg
      541 catatttttt ccttaatctt taggaatttt ttattattta ctttggggcc tggagacagg
      601 aaaaattatg ctgacattca ttgaactcct tattggggtt gtggttattg tgggtgtagc
      661 tcgctacatc attaaagggt attccgccac tggtgtgtta tttgtcggtg gcctgttatt
      721 gctgattatc agtgccatta tggggcacaa agtgttaccg tccagccagg cttcaacagg
      781 ctacagcgcc acggatatcg ttgaatacgt taaaatatta ctaatgagcc gcggcggcga
      841 cctcggcatg atgattatga tgctgtgtgg atttgccgct tacatgaccc atatcggcgc
      901 gaatgatatg gtggtcaagc tggcgtcaaa accattgcag tatattaact ccccttacct
      961 gctgatgatt gccgcctatt ttgtcgcctg tctgatgtct ctggccgtct cttccgcaac
     1021 cggtctgggt gttttgctga tggcaaccct atttccggtg atggtaaacg ttggtatcag
     1081 tcgtggcgca gctgctgcca tttgtgcctc cccggcggcg attattctcg caccgacttc
     1141 aggggatgtg gtgctggcgg cgcaagcttc cgaaatgtcg ctgattgact tcgccttcaa
     1201 aacgacgctg cctatctcaa ttgctgcaat tatcggcatg gcgatcgccc acttcttctg
     1261 gcaacgttat ctggataaaa aagagcacat ctctcatgaa atgttagatg tcagtgaaat
     1321 caccaccact gctcctgcgt tttatgccat tttgccgttc acgccgatca tcggtgtact
     1381 gatttttgac ggtaaatggg gtccgcaatt acacatcatc actattctgg tgatttgtat
     1441 gctgattgcc tccattctgg agttcctccg cagctttaat acccagaaag ttttctctgg
     1501 tctggaagtg gcttatcgcg ggatggcaga tgcgtttgct aacgtggtga tgctgctggt
     1561 tgccgctggg gtattcgctc aggggcttag caccatcggc tttattcaaa gtctgatttc
     1621 tatcgctacc tcgtttggtt cggcgagtat catcctgatg ctggtattgg tgattctgac
     1681 aatactggcg gcagtcacga ccggttcagg caatgcgccg ttttatgcgt ttgttgagat
     1741 gatcccgaaa ctggcgcact cttccggcat taacccggcg tatttgacta tcccgatgct
     1801 gcaggcgtca aaccttggcc gtaccctttc gcccgtttct ggcgtagtcg ttgcggttgc
     1861 cgggatggcg aagatctcgc cgtttgaagt cgtaaaacgc acctcggtac cggtgcttgt
     1921 tggtttggtg attgttatcg ttgctacaga gctgatggtg ccaggaacgg cagcagcggt
     1981 cacaggcaag taaatagtaa tgccggaggc aagtttcctc cggctttatc atgtcaccca
     2041 ctgtggtata tgcgttgtgg tctgccaact ttgccgtgaa caatttcagc aataatcaga
     2101 tggcggctgg cgcaatattc aagataacgt ctggcagtgg tgcggctgat ggtcagtgcc
     2161 tgcgccaccg tttctgccgt atgttgcaca ccaggctctt taaacagttt tcgcaccgcg
     2221 tttagcgtca agggatcaat gccggtcggc agttcgtctt taggttcacc gcgagcatag
     2281 gcattaaaca tctcatcaat ttgcttctgg ctggcgctat caatactttc cagcatatgt
     2341 ttacgctggc ggaaacgggt tagcgtttgc cccagccgtt cataggcaat gggcttaatg
     2401 agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc gctggctgca
     2461 gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcaa taaattaatc
     2521 cctctgccgt caggaagata gttatcgagc aagattagcc ccggcttaaa acgctcgatc
     2581 atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc gggaatgtga
     2641 cgaatatatt ccgcatgcat ctctgccagc ggcgttttgt cctcaacgat caatagggtt
     2701 aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg taccgcaggg
     2761 atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg caatcaagta
     2821 caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgaccccct gctcaaatat
     2881 tttgtctcgt agagactctg gaacgccgca gccctgatcg gcgacttcaa tcaccacatc
     2941 atcgccttca tcgctgagga ataattcaac gatcttgttt ccttcatcgc tacgcaggct
     3001 ggcttcgaag gcgttatcaa gtaaattgcc cacaatggct gcaaactcgg tgctatccag
     3061 tcctagcggc agttgcgaaa gctggctacc ggggacaatg atcattttta gccccagttc
     3121 ccgggcgcgc tgcactttac caaaaagcag ccccgccacc tggcgatcgg caaacgcctc
     3181 gcgcaggctg tcaataagct gttgctgggc ctgagactcc ccctgcacca tcgccagcac
     3241 gcgatcatac tctttcatct gcaacagacc attgagcgtc gacatccaat tgagatgctc
     3301 gtgtcgcaat gtacgaaggc tctcaacgta ttgttttatt tgcgtcagtt gcgcattgag
     3361 ggtggaaatt tcgtctttac tgcgaaagct gataatggcc cccagcaaat catcacctga
     3421 acgaatagct tcccgattgg caataacgct cagaccgtta aagttagcca ccacatcctg
     3481 acgtttttca tcaatctgtt cggtaaagaa atcggcgggc ctgaccactt cagcaatggg
     3541 tttacccaac cattgccgtc cgggggaact cagccccagc atctttcttg cgttacgatt
     3601 gatggcggta atgtaaccgt gcggatccac cgcaatcagc ccttcataaa ccgaactaaa
     3661 cagcgcctct tgctgacgga ccacgcgtgc gatttgcttt ggctccatgc ccatcatctg
     3721 ccgacggata tgcgcggcca ggaaccacga cagcaacatc agaatcccta acagcacgac
     3781 aaacacacct gccatcggta ataaaaactc agcccgccag ctatcgattt tactcaccag
     3841 gtagccaatc gacaccacgc cgatgacttt tccatcgtca tcaaagattg gcgttttggc
     3901 gcgcatcgcc atccccattg accctttccc ggtaatgaag tagctctccc ctttctccag
     3961 cgcgccctgt ttggtgaact gcataggata accaattttc tccggattag gatggtaaag
     4021 gcggatcgag tgccggtcgc caatcaccac ataatcaaaa tcggtatctc tttgtaattt
     4081 gttagcgatg gtcgccatcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
     4141 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
     4201 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
     4261 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
     4321 attctcgtta agctgcaaca ttgttttcct tgcgggttgt cccgagcctc tttgacagat
     4381 gtaattaaaa tgttgaatca aaatacatat atattaatta tgatcgtggc aaattttatt
     4441 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
     4501 acttaaacca ttaaataaat aaataaataa ataaataaat aaataaaaaa ccactaactc
     4561 tatgtgaaat aaatcaaaat ttcacgccga aatactcctt aggatgtata gcgaaaggag
     4621 aaaaagatat acctcgatca cccccctttc tcccaagtga aaataaaagg ttatcagttt
     4681 gcaacattga acaacattcg ttgcaaatcg ataacaacat gcaccttcag gatactattt
     4741 attatgttcg gcaatgatat tttcacccgc gtaaaacgtt cagaaaataa aaaaatggcg
     4801 gaaatcgccc aattcctgca tgaaaatgat ttgagcgttg acaccacagt cgaagtattt
     4861 attaccgtaa cccgcgatga aaagcttatc gcgtgcggtg gaattgccgg aaatattatt
     4921 aaatgcgttg ctatcagtga atccgttcgc ggtgaaggac tggcgctgac attagccact
     4981 gaactgataa acctcgccta tgagcggcac agcatgcatc tgtttattta taccaaaacc
     5041 gaatacgagg cgctgttccg ccagtgcggt ttttccacgc tgaccagcgt acccggcgtg
     5101 atggtgctga tggaaaacag cgccacacga ctgaaacgct atgccgaatc gctgaaaaaa
     5161 tttcgtcatc cagggaacaa gattggctgc attgtgatga acgccaatcc ctttacgaat
     5221 ggtcaccgtt atctgattca acaagctgcg gcacagtgcg actggttgca tctgttttta
     5281 gtcaaagaag attcttcacg cttcccctat gaagaccggc tggatctggt gttaaaaggc
     5341 accgccgata ttccacgcct gactgtgcat cgcggctccg aatacatcat ctcccgcgct
     5401 acgttccctt gctacttcat taaagaacag agcgtcatta accattgtta caccgaaatt
     5461 gatctgaaga ttttccgtca gtacctcgct cccgcactgg gtgtaactca ccgctttgtc
     5521 ggtactgaac ccttttgtcg cgttaccgcc cagtacaacc aggatatgcg ctactggctg
     5581 aaaacgccga ctatctccgc accgcccatc gaactggttg aaattgagcg gctgcgttac
     5641 caggagatgc cgatatccgc ttcccgggta cgtcaactgc tggcgaaaaa cgatctcacg
     5701 gctatcgcgc cgctggtccc tgcagtcacg ctgcattatt tgcagaacct gcttgagcac
     5761 tcccgccagg acgcggcagc tcgtcaaaag acccccgcat gagaaacagg tgaaaaatga
     5821 aaataaacca gcccgccgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
     5881 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
     5941 gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac cgtacatggt
     6001 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
     6061 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcgcacgc cagccggatg
     6121 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
     6181 ccaggagctg gcggacgttc ctgcctagaa cgctacgaat atgccaaaca cggtgcctgc
     6241 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
     6301 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
     6361 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
     6421 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
     6481 aacgctccgc tttctgcaaa ctcattcttg ccacaaccac acccaggcaa ctgtggcaaa
     6541 acctttgtga ttgataaagt gggttattaa tcatctgatt tacaaaacac gctaattcgt
     6601 cttccataaa ccgtgacgat taagaattat acataatgcg ctgcattgag gaattccctg
     6661 aatgtgacgc aaatcacttc aaagagtgag caacagtcag tatcatcatg tgagttaaac
     6721 cctcgccgcc tgacggtgag ggttttcttt tgggtatgtt tatctgcgac actcgcagta
     6781 ccgacgacat ggagtaaaaa tgtccagacc aactatcatc attaacgacc tggatgccga
     6841 acgcatcgat attctgctgg agcaccccgc ctatgctggt ttgccaatcg ccgactcgtt
     6901 aaacgcagag ttggatcgcg cccaaatgtg ttcgccagaa gagatgccac acgacgtggt
     6961 gacaatgaac agccgggtta aattccgcaa tcttagcgat ggcgaagtgc gtgtgcgcac
     7021 gctggtgtat ccggcaaaaa tgaccgatag caatactcag ctttccgtta tggctccggt
     7081 aggtgccgca ctgctggggc tgcgcgttgg cgattccatt cactgggaac ttccgggcgg
     7141 cgttgcaacc caccttgaag tgctggaact cgaataccag ccagaagctg ctggcgacta
     7201 cctgctttaa tcccatctgc acaagcgata actcgctttg cccagaggat gcactcgcat
     7261 cctctttacg caaaatcagc aatttttcct ggttctcact gttcactttt gctgcaaaat
     7321 tattccgttt cgtttgtggg aataaccatg gcagaagcat tttacatcct gataggattt
     7381 ctgattatgg cggcgattat cgtcatggct gtcctttacc tcgaaaacca ctcctgacga
     7441 tcatattgtt ccccccggca tcgcattcac cagacctgaa actgccttct gcgccgcctc
     7501 tgcgctttgt gcaccgggta ccagaatcac cttacggcac tcctcttcac gtttttcgaa
     7561 aatctcatat ccccgggcgg cctcttcaaa cggcatatag tgggtaacaa tttcttctgg
     7621 tttcagtaat cctttctcaa ttaacggtaa taattctccc agccatgcgt gaacgtgggt
     7681 ctgtcccatt ttaaacgtca accctttatc aaaggcgtca ccaaacagga aaccgtgaat
     7741 aaatccagcg tagacgcccg gtacgctaac aatgccgcca cgcctgaccg ccgcaataca
     7801 ctggcgcaac gctttaccgc tgctgccctc ca
//
LOCUS       DAJTMK010000170         7823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTMK010000170 DAJTMK010000000
VERSION     DAJTMK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.174"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            269..412
                     /locus_tag="OO135_003404"
     CDS             269..412
                     /locus_tag="OO135_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5399135.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            425..634
                     /gene="cspE"
                     /locus_tag="OO135_003405"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             425..634
                     /gene="cspE"
                     /locus_tag="OO135_003405"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5399136.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            800..1609
                     /gene="rlmA"
                     /locus_tag="OO135_003406"
     CDS             800..1609
                     /gene="rlmA"
                     /locus_tag="OO135_003406"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5399137.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTCGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1606..2172)
                     /gene="mntP"
                     /locus_tag="OO135_003407"
     CDS             complement(1606..2172)
                     /gene="mntP"
                     /locus_tag="OO135_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5399138.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2600..3058)
                     /locus_tag="OO135_003408"
     CDS             complement(2600..3058)
                     /locus_tag="OO135_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5399139.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLEKIYKLIVSTQ"
     gene            complement(3113..3964)
                     /gene="manZ"
                     /locus_tag="OO135_003409"
     CDS             complement(3113..3964)
                     /gene="manZ"
                     /locus_tag="OO135_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5399140.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3977..4777)
                     /gene="manY"
                     /locus_tag="OO135_003410"
     CDS             complement(3977..4777)
                     /gene="manY"
                     /locus_tag="OO135_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5399141.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4840..5811)
                     /gene="manX"
                     /locus_tag="OO135_003411"
     CDS             complement(4840..5811)
                     /gene="manX"
                     /locus_tag="OO135_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5399142.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6274..>7823
                     /gene="yoaE"
                     /locus_tag="OO135_003412"
     CDS             6274..>7823
                     /gene="yoaE"
                     /locus_tag="OO135_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5399143.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDE"
BASE COUNT         1988 a         1915 c         1945 g         1975 t
ORIGIN      
        1 cgcactatgt aataacttct cccactggcc tggaacaact gaacttattg aactatgtta
       61 gaaaatacgc cagtttaagt atctgcctga actggcaagg ttaagcacaa tgatatatcg
      121 gcgcgtattc cgttgcataa gtgtgcaaaa aaagtggaag acgtatcgag atttgtgcgt
      181 ctgatcgaga catgtttaaa aatggcttgc cataattaac gttgtatgtg ataacagatt
      241 tcgggttaaa cgaggtacag ttctgtttat gtgtggcatt ttcagtaaag aagtcctgag
      301 taaacacgtt gatgttgaat accgcttctc tgccgagcct tatattggtg cctcatgcag
      361 taatgtgtca gttctatcta tgttatgcct gcgggcgaag aaaacaatct aaggaatttt
      421 tcaaatggca aagattaaag gtcaggttaa gtggttcaac gagtctaaag gttttggctt
      481 cattactccg gctgatggca gcaaagacgt gttcgtacac ttctccgcta tccagggtaa
      541 tggcttcaaa actctggctg aaggtcagaa cgttgagttc gaaattcagg acggccagaa
      601 aggtccggca gctgttaacg taacagctat ctgatcgaat ccactgatct gaagtgtgaa
      661 tacgcttcaa tctcgctata aagcctcgtc gaatgcgagg ctttttacta tgctttatct
      721 tcgctcctgg cgttcggata tttgcccgcc gcgtgattcg cgttacactt gcggccttta
      781 gtatcctgcc ggagttgtaa tgtctttttc ctgtccactt tgccatcagc ctctttcgcg
      841 tgaaaaaaac agctatatct gtccccagcg acatcagttt gatatggcga aagaagggta
      901 tgtcaatctg ctgcccgttc agcataaacg gtctcgtgat ccgggcgaca gcgcggaaat
      961 gatgcaagca cgccgcgcat tcttagatgc cggacattat cagccgctgc gtgatgcaat
     1021 tgtcgcccaa ctgagggaac ggcttgatga taaggccacg gcggtgctgg atattggctg
     1081 tggtgaaggg tattacacac acgcatttgc cgatgcgttg cccgaaatca ccacgtgtgg
     1141 tctggatgtt tcgaaggtag cgataaaagc ggcggcgaaa cgctatccgc aggtcacttt
     1201 ttgtgtcgct tccagccacc gtttgccgtt ttccgatacc agtatggacg ccataatacg
     1261 tatttacgcg ccgtgtaaag cagaagaatt agcacgagta gtgaagcccg gcggctgggt
     1321 cattactgcc acgccgggac cgcgacattt gatggagctg aaggggctga tttacaatga
     1381 agtacatctt catgcacctc atgcagaaca actggaaggt tttacattac agcagagtgc
     1441 ggagttgtgt tatccgatgc gtcttcgcgg tgatgaagcc gtcgcattat tgcagatgac
     1501 gccgtttgcc tggcgtgcga agccagaagt ctggcaaaca ctggcagcaa aagaagtgtt
     1561 cgactgccag acggacttta atattcacct ctggcagcgt tcttattaac cgtggaagtg
     1621 cgtccagagg atctggacgc cgatgccgat cagtaccagc ccgccgagaa tttccgcttt
     1681 tttcccaata attgagccga taaagcgacc aaccatcatc cctaatgttg acataatcaa
     1741 ggttgcacaa ccaatggcca atgcggtcgc gataatgttg acctgcagga aagcaagacc
     1801 aacacccaca gccatggcat ccaggctggt ggcaatcgcg gtggttacca gtagccagaa
     1861 accgtgtcga cggcgcggct cttcatcttc atcatctgcg ccacgaaaac cctcaataat
     1921 cattcgcccg ccgaggaata tcagcagcac aaacgcaatc cagtggttcc attcaaggac
     1981 aaaccggctg gctaacatgc ccattcccca gccgatcagc ggcgtcaggg tttcgacggc
     2041 accaaaaata aggccggttc gcaatgcttc agaaaatttc ggtttatgga gggtggcacc
     2101 tttaccgatt gatgcagcaa atgcatccat cgacatacca aacgcaagaa gaacagtagc
     2161 agtgatattc atgacaatgt cctgaccggg gtatccataa aacacatcat cgcccccagc
     2221 aaacatgcat acatgaacaa caagccgtga tggttttcgc gttcatgcag gcatagcgat
     2281 tgatgtgcat atggtctcgc ctgactgaaa gtattcagtc cgtacgtgcc acgttttgca
     2341 acgagtatgt tgacacgtac atttccggaa tctggaaatc ggctactccc caaatgaggg
     2401 cgcaacctta acatattttg agaatataaa acaactgttg gaatatatta tttcattgct
     2461 ttattgataa cgaattcatt tagatttata tatcaatgaa acgttaaaat aaataacaaa
     2521 taagggaaga ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag
     2581 ctttggctaa attcataact tattgagttg aaacgataag cttataaatt ttttccagat
     2641 tgtcgatatt tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc
     2701 catcttccga caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga
     2761 aaaaaccttt ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca
     2821 tcagagccag tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt
     2881 tatagataag aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga
     2941 gcaaaggaat tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga
     3001 tcgcgaaggc caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta
     3061 atcctccaga taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc
     3121 ccagcaggcc gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca
     3181 gcgggttaac ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca
     3241 ggcctggcat taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct
     3301 ggtcagtaat gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg
     3361 cccccatgac aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac
     3421 cacccatatc tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac
     3481 gggttgccag acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc
     3541 cgctcatcgc gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga
     3601 tcgggtcgcc tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac
     3661 cgtcgtcgat ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa
     3721 tcggcgcagc cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt
     3781 tacgagcttc gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga
     3841 aacccagtgc ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga
     3901 agacgccacg aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa
     3961 ccatttcgct cacctgttag tccagttcgt tatcgagatc gttgttacca gcagcctgag
     4021 caggcgcacc ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa
     4081 cagtaccgat cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc
     4141 cgaggtagaa gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac
     4201 caaccaccac gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg
     4261 cattcagcat gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa
     4321 tagccacacg cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca
     4381 ggttgccgtt atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa
     4441 cgatgatggt cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac
     4501 caatgctctg atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt
     4561 caggcgcaac tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac
     4621 cgataataat accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta
     4681 gcggacggtg aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga
     4741 taaataccag cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag
     4801 acttaagtgt gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat
     4861 cagatccatc attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc
     4921 gcgcgcattc agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt
     4981 cacctgggtt ttaccttgac ggaatgccat accaccgacg ttaacagagg tgattttcac
     5041 gccgccttca acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc
     5101 gccagcatat ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc
     5161 tgttacgccc ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac
     5221 ttcatcacta acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac
     5281 ctgaccgtga atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg
     5341 ccccattggt tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc
     5401 ggctttttca accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc
     5461 cagcgcaacc agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag
     5521 cattggaatg ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct
     5581 ggcagcattg aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt
     5641 gtcgagtttt gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg
     5701 aacgaaatcg atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag
     5761 caactgctct gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct
     5821 cctttattat cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc
     5881 gaatcgattc agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat
     5941 atcgaaaaaa ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt
     6001 tcgaaaggta aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat
     6061 cagaggcgtc tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc
     6121 ccgcagtatg gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc
     6181 tggcactcta tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc
     6241 gtcgaatagc tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt
     6301 tgggcggggc tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc
     6361 ttcatcgcca ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg
     6421 gggttatcac tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc
     6481 acgctgacca aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt
     6541 atgttgttcg gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg
     6601 gaaaaccgcg atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc
     6661 acacagattg tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg
     6721 atggttaacc atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg
     6781 ctggcatcca aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt
     6841 ctgagcttcc tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt
     6901 ccgaaaggtt acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag
     6961 attgcgcgtc gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc
     7021 gatgcaatcc tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat
     7081 aacccgatgc cgatgccgat cccggaaggt gcatttgccg aagaagaacg ttacatgatt
     7141 aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga tcatgacgcc gcgcggtgaa
     7201 ataagctggg ttgacgctaa tctcggggtc gatgaaatcc gcgagcaact gctctcttca
     7261 ccgcacagtc tgttcccggt atgtcgcggt gaactggatg aaatcatcgg tattgtacgt
     7321 gctaaagaac tgctggtggc gctggaagag ggcgttgatg tggcggcgat tgcttcggcg
     7381 tctccggcga ttatcgtccc ggaaaccctc gatccgatca acctgttggg cgtgctgcgt
     7441 cgtgctcgcg ggagctttgt tatcgtgacc aacgagtttg gtgtggtaca aggtctggtc
     7501 acgccgctgg atgtgctgga agccattgcg ggtgaattcc cggacgctga cgaaacgccg
     7561 gaaatcatta ctgatggtga cggctggctg gtaaaaggcg gtacagattt gcatgccttg
     7621 cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg atatcgcgac ggtcgcgggc
     7681 ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg atgtgattga tgtagggcca
     7741 ctgcatatca ccatcattga agccaatgat tatcgtgttg atctggttcg cattgttaaa
     7801 gagcaaccgg cgcacgatga aga
//
LOCUS       DAJTMK010000171         7801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTMK010000171 DAJTMK010000000
VERSION     DAJTMK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7801
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.175"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..360)
                     /locus_tag="OO135_003413"
                     /pseudo
     CDS             complement(1..360)
                     /locus_tag="OO135_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(700..1254)
                     /locus_tag="OO135_003414"
                     /pseudo
     CDS             complement(700..1254)
                     /locus_tag="OO135_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1397..1519
                     /locus_tag="OO135_003415"
                     /pseudo
     CDS             1397..1519
                     /locus_tag="OO135_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="OO135_003416"
     CDS             complement(1781..2347)
                     /gene="ipgB2"
                     /locus_tag="OO135_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5399144.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANIISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2629..2943)
                     /locus_tag="OO135_003417"
     CDS             complement(2629..2943)
                     /locus_tag="OO135_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399145.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3021..3131)
                     /locus_tag="OO135_003418"
                     /pseudo
     CDS             complement(3021..3131)
                     /locus_tag="OO135_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="OO135_003419"
     CDS             complement(3455..4132)
                     /gene="ospD1"
                     /locus_tag="OO135_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5399146.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4503..4640)
                     /locus_tag="OO135_003420"
                     /pseudo
     CDS             complement(4503..4640)
                     /locus_tag="OO135_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4722..5020)
                     /locus_tag="OO135_003421"
                     /pseudo
     CDS             complement(4722..5020)
                     /locus_tag="OO135_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5609..5768)
                     /locus_tag="OO135_003422"
                     /pseudo
     CDS             complement(5609..5768)
                     /locus_tag="OO135_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5772..6926)
                     /locus_tag="OO135_003423"
                     /pseudo
     CDS             complement(5772..6926)
                     /locus_tag="OO135_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6946..7293)
                     /gene="tnpB"
                     /locus_tag="OO135_003424"
     CDS             complement(6946..7293)
                     /gene="tnpB"
                     /locus_tag="OO135_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5399147.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7293..7669)
                     /locus_tag="OO135_003425"
                     /pseudo
     CDS             complement(7293..7669)
                     /locus_tag="OO135_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2007 a         1811 c         1709 g         2274 t
ORIGIN      
        1 tgttgttgcg ccttgctgcc aacgaagctc cactgcccat caagttcaca gataagggca
       61 acatcagcgt gagcgactgg cgcagatgtt atccgccggg gcgggagctt tttaatgtcc
      121 gtatgacggt attgatgccg attttcagcg tacgggcagt atcgcgaact ccggcaccgt
      181 tgaagccatt tcagtgattt ggtttttaac tcccggttta cgggcctcgt aacagtaagt
      241 gagctgaaac acccggtgac agtcacggca acggaatcga tcatgtcctt ttgggttctg
      301 cccgtgacgg taaacctgaa cggactggca acgggggcaa tgaaagttaa ctgtgaccat
      361 gaaagaacct taaaagcggg tattataacc cctaattcaa ctaattggag gcattgtcat
      421 ttttaatcta tggatacact taaaaatcct ctcaccttac gttaaaaacc ttctcatgtt
      481 tagagttttt tattatgttt atcataatga tatgacgatc tgagttttta aaatcaagaa
      541 gaaaagataa gttttttaaa tgcacagtgc ctgtggataa ctttgcgtaa atccggagga
      601 cagatcactc tgaagcaaga caacagcatc tgaaaacaat atgctcacgt ttcaaccaca
      661 gggcgtaaat gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
      721 aaattgttcc ggactgaggc cgccacacca actgtgccgt cgccagcgat tgtaatcaca
      781 ttcgatataa ttaaacactg ttgcccgcat tatttcccgg ctgataaagt gttctccgtg
      841 gagacattcc actttcagcg aatgaaagaa gctttccacg caggcattat cgtagcagca
      901 gccctttgcg ctcatactgc cacgcagatt atgccacttc agcagcgcct gataatctgc
      961 tgaacagtac tgacttcctc ggtccgagtg aacgataacg ttccggggtc tcttacgccg
     1021 ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc gtcaggcgtg gtgacatgga
     1081 ccagccaata acggcacgtg accacaggtc aatgaccaca gccagataca gccagccttc
     1141 atctgtacgt aagtacgtga tgtctcccgc ccacttctgg ttcggaccac tggcgtaaaa
     1201 atcctgctcc agcagatttt ctgacacagg caggccatgt gcgcggtaac tgaccgtatt
     1261 gctgggtaag atcaatttgc tcactggtat ttactacgtg catcgaatga gtgatcaaga
     1321 gccgtatgac gcgagagtgt cacgtacggt tccgtgagca gccggagagt gaaactctct
     1381 ccggctgact cgactcgcag gttccgacag cggtgacgaa catgcagcgg tgttgttctc
     1441 gctgatcggc acatgccgtc agaacaatgt ggagccagaa aaatggctgc gttacgttat
     1501 tgaacatatc caggactgac cggcaaaccg ggtacgcgat ctgttgcctt ggaaagttga
     1561 tctgacttct cagtaaatat caatatgatt ctgacgagcc gcttaccttt gagcagagaa
     1621 aggggagagg gatcccacat ccatgagcta ggaaaataca aaaactggct atacaacaat
     1681 tcaatatatt aggacttact tgcgaggaga aatatagcac cgctcaggca attcctgggt
     1741 atttactttt cttagtttct ttactcggga tgttctgtca tcagaaaggt gattctaaat
     1801 ttgtaatata gtcactcata ttcccacgta atttctttag atcaatatat tgcacatttt
     1861 tctcaaaaaa ctgatcggaa ataatatttg caacattgct tgttgtatca tttttaacgc
     1921 ttctattcat cccttcacaa agttcgtcca ttttcttttt gaaataactg cttcctgata
     1981 caagatgatt tatggaactt tgtgcagcat tgatgtctag agaaatgtga taatctttct
     2041 caacctgttt aaatatctgt ttacggagat tatcgccgat ttttcggctg tgttctgcac
     2101 aaaacttatc aatacattgg tttaccaccc gggatacgta gacttttttt tcatcatttt
     2161 tccaatctgt aatattttca ccgatttgtt ctgaaacaga gctcaaaata ctagtattta
     2221 ttttggttga tgatattttt actatccttt tccccattgt acagcggata atctcatcaa
     2281 cctttcctga aaaatatctt ttgtgagata aactgattcc aaaattatta aaagatgttc
     2341 caagcattat aatcactttg tcatgtaaaa tagtaacatt gtcattattg tagctgattc
     2401 aaacaattct gtaaatgctt tgagttcttt actcccttgg ctcaatgatg gaccgtctgg
     2461 aatcccagag actaaactct ctgagaaaga gagtatgaaa aaacttgggg cagtccctaa
     2521 atgaagggac caacagcggt aaaataaccg gcatcattca taatgatgcc ctgttagttg
     2581 tagttatgaa gatttacgtc attaaaattg ctgttcgtag tgtcagtcct atggtctggc
     2641 atcagctgag gatcgtagcc gatacatcac tggcttcacg acattcgtgt tgaggctatt
     2701 catgaaaact ccacgctgaa aacgccgttt tgtataaacg gtcacggtat gcccggagcc
     2761 actgttgctg atgaattcga taaaaccttg gcgtttcttg aagctatcgt taatgcggat
     2821 aatgaaacaa cgattggtga gattcgctct tttgccgaca ccctggacgc tgtccggttc
     2881 aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc ttgcctctct ggcgctggag
     2941 catgaagtga tttagctggg acgtagccac tgacagcttc tataaaagta tgatcctacc
     3001 tacgtaatat gaacacaggc ttaagtgagg ttctggtttt caaattgttc aggactgaga
     3061 cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc acttgatgta attaaacact
     3121 gtatatttca tttgtggagt ctccagtctg agggggcttt atgcattccg tattgggcac
     3181 tatgatgcca gaaatctgca aggctccaca ccttcctgta tttcccttcc cctatcacag
     3241 tgcactttac ttaatagggg ggaatgtgtt cattacattt attgtgtttt ttatgattgt
     3301 ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta tgggtagtat cgctcagtct
     3361 agttaactgt ttgtttcttt ttagaaatta agcagatcac acaattcggg gagagatcct
     3421 tctaagtgca attctttgca gctatttagc acacttaaaa caactcttgt attttgtcag
     3481 aaataatatt aaacatgtct tgttcattat tgttaagtct gtctgctatt tctgtaagtt
     3541 ttatcccact atgatggcat atatttatat atgccatcat agcctcgata ttccctttcc
     3601 ctgcggccag aaataatcct ggtaacccct cattatctct tacatccaat agtttaatcg
     3661 tttcttttgg tgataaatca aaagttttaa taaaatctcc ataagcttga atcgcagcgg
     3721 aatgaccttc ttgaagagct atgaacaaag cagattcccc agcattattt cgtgctgcaa
     3781 gaacttctag tttttcctct ttgggtattt cggctgccaa cagcgattca ccgtatttac
     3841 gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa attatggcgg ttatactcct
     3901 tattattctt tgtcggaatg gatatgctct gtattttggc caattttctg aaagaaacac
     3961 cactatatcc cccccctgat ttttcttcat tgatggcgtg ggatatagcg gtatttgtca
     4021 cgttaatgat attacgtttc attcctttat tttcattagc agtattgctg cctattatta
     4081 ggtgcatatt tttgttgttt gctggatgta atccatagtt atttattgac atttttgttc
     4141 atcctttttg ctaaaaaaaa caataatcta tttttgtaat gtaatagata ttagtctatt
     4201 gctaatctaa aaaaaccatt gccacattga atggctatat tcaccaataa attctttcga
     4261 tttcgtatat tgacatatcg tagatatatc ttatccctaa gattcaataa tcgagtccgt
     4321 ttaccattct gtgcaagcgc cttcactcag aagtgaccgt caaggcgatc accaaacttg
     4381 ataataaagc agctcattgc catacgccag tccttaaggg gcgttgtcca tttctgtgat
     4441 gctgcctaga tttaactgcc agctccggct tatctacatc taaatatgtg agcctaatac
     4501 cgctacactt tggccagact atgtttttct ccgggaagtg ggctaaccgt ctccataaag
     4561 tgacgaactt ttttcaacag tggccatatt gaacggcact gatgattacg ggttattgtc
     4621 tcatgaagag ccggccacat cactgacagc agtgacttca ggccttcagc accagacagc
     4681 ctgaaccagt taatccatcg cccgacagat ttagtaatct agggctggtt tttcttcagc
     4741 tgttgcggtt cgaaagtgcc attacgatca cgcagagtac ggatttccag aggttcatct
     4801 ccagtagtga taatcttcgt taagcaacca ttgcgggaat tgctgccaga tctaagctga
     4861 tttttctcgc acccaagatg atgagtcata ttagcattga gtacggtttc attttcagta
     4921 tccaatcaaa ctgactgaga tcttcaggat ttttgagact tttggctagt tcattagcta
     4981 aagcccgcaa cttattagat gtcaactgtt tttcattcat aaattcacct tcatttgata
     5041 tcaaattaaa tagttcaaca tcagagaatt atacaaactt atgtacaggc tcctatgatc
     5101 ttcgaatcct gaagattata gtgaaaataa gttttcacgt tgaaatccca catagttagt
     5161 atatgataaa taatcatgga tgacctgatt aacctcttgg ggtagtgctt ttatagaaga
     5221 ataaatattc tactaattta atacatcacc taaaatagta ttgtttttta tggttggttg
     5281 aaggtcgtgg tgatgatgtt agtccttttg agaatattcc agtgccagat cttacttata
     5341 ataaaggggc ttggcagttt ggataaacta ttgttcttat tccactaata caaacttttt
     5401 gtaaagtata ttaaatatac ttcatttcat actgaaatcc ccggaagtat tcaatcaaac
     5461 aattacacca taaaaggaag agggggccct aattaagttg tcaagcatgt ttatgatacc
     5521 tgcagggtat aaattttctg gctgcaagca gacacatata aaacgtcttt attgatccta
     5581 cccacataat atggacacag tcgtaagtga ggctctggtt ttcaaattgt tccggactga
     5641 gaccaccaca ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taactaaaca
     5701 ctgttgcccg cactatttcc cgatggataa tagtgttctc cgtggagaca ttccattttc
     5761 agcgaatgaa ttttcatctg ttgctgtatc caatcataca gtgactgcat cagtggcgcg
     5821 gctctggctt ttcttgccgc cagacgctgt tctgctgaac atccccggac ttctgcctcg
     5881 atggcataca gttcaccgat acgcagcagg gcttccgtta ttctgccgga ttcgtacaac
     5941 gcccggtaac caccgtaagc atcggcctga agcactccgc tgtaaccggc cagatgattc
     6001 tgtggatgga tacctttccg gtccggtgag tacgcgaacc agaccgccgg aggcatttcc
     6061 gaaccggcgt tacggtcatc acgaacgtag acccacagcc gggcagtcct tgttttaccg
     6121 ctgcccggct cctggaccgg aacggggata tcatcggcat ggactttacc gggcatcagc
     6181 acatactggc gcaggatgtc atacagcggt tccagcagtt cagcaacagc acctgcccag
     6241 cgccccagtg tggcacggct cagctccacg ccctgacgac ggtatatttc tgactggcgg
     6301 tataacggca gatagtctgc atattttcca gcgacaacat gggccagaag ccccgctccg
     6361 gcataactgc gtgcaatggg gtttgaaggc accggtgcct gcacgatatg gtcacaccgg
     6421 caacaggcca gtttcggacg ttgtgtttcg ataaccttaa aggcgctgct gataagctcc
     6481 agctgctctg acacatcaca tcccagagaa ctgagttcgc caccacaggc cgggcagcat
     6541 tcctcttccg gacggatgac ccgggtttca cggggaagtg aggcaggtaa cggtttacgg
     6601 gctgaagact ggcgcagggg agatggcagt accgggtcat attgctcacc cagcgtttcc
     6661 gccatttctt cctgaagtgc gctgattcgc tcctgtgctt ccagtatctg ccgttcggtt
     6721 tttgcgcgaa gtttttctga gcttttaccg aactgcatgc gctgcagttt cgcaaccagc
     6781 gccttcagcc ggttgatttc ggaagcataa gccgccaccc gctgtgagag caggcggttg
     6841 tatttcgcca tctggcggat ggtgtcctgt tgcgtctgca acagtgcccg caggcgggca
     6901 ttctcatgag caagtgaggt gtccatatcc tcactttaca acgggttata tgcggatgcc
     6961 agcgcgttcc gttcgtttcg ggtgtttcca gtcgatgccc tcaagaagca tggataactg
     7021 ggccggagta aggtgcacct tgccgtcacg ggtgaccggc cagacgaagc ggccccgctc
     7081 caggcgtctg gtaaagaggc acagtccgtc actgtcagcc cacaacactt ttatctggtc
     7141 accccggcgt ccgcggaaga tgaacagatg cccggagaac gggtcatcct tcaggacgtt
     7201 ctgaactttt gaggccaggc cgttaaagcc atttcgcata tcggtgatac ctgcaaccag
     7261 ccagatacgc gaacctgcag ggaaagatat catcagtggc tgctcccttt tatttcgcgg
     7321 ataagtatct gtaataacgc cggcgtcagt ttacctttaa gcctgagagt tccggccggc
     7381 agaaccagct cacagcacag actgtcggac ggtgtattta tctgctctgg ttcctgtgcc
     7441 ggagccggga ttttattatc cggctccggc gttaacgtca cgggaagcag tgcaggcata
     7501 tttttccgga aggcagcagg ccacctttcc ggtattgatg gcgccagttg aagagcaggt
     7561 tatcgttgat tccgttttca cgggcgatct gcgccacaca ggctccgggc tgcagtgact
     7621 gctccactaa ggcgatttta aattcataag gaaaattagg ccgccggggt aatgccgatc
     7681 agttaaggat cagttgaccg atccagtggc tgtgtaagaa tccggaaacg ctcacttgtt
     7741 tccggatttt tttatgcaca ttggacaggc tcttgatctg gtatcccgtt acgattctct
     7801 g
//
LOCUS       DAJTMK010000172         7792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTMK010000172 DAJTMK010000000
VERSION     DAJTMK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.176"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            118..297
                     /locus_tag="OO135_003426"
                     /pseudo
     CDS             118..297
                     /locus_tag="OO135_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            823..2001
                     /gene="ltrA"
                     /locus_tag="OO135_003427"
     CDS             823..2001
                     /gene="ltrA"
                     /locus_tag="OO135_003427"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5399148.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2084..2263
                     /locus_tag="OO135_003428"
                     /pseudo
     CDS             2084..2263
                     /locus_tag="OO135_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2293..2775)
                     /locus_tag="OO135_003429"
                     /pseudo
     CDS             complement(2293..2775)
                     /locus_tag="OO135_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3031..4011)
                     /locus_tag="OO135_003430"
     CDS             complement(3031..4011)
                     /locus_tag="OO135_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5399149.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVMKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4011..5210)
                     /locus_tag="OO135_003431"
     CDS             complement(4011..5210)
                     /locus_tag="OO135_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5399150.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5532..5870)
                     /locus_tag="OO135_003432"
                     /pseudo
     CDS             complement(5532..5870)
                     /locus_tag="OO135_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5937..6719)
                     /gene="istB"
                     /locus_tag="OO135_003433"
                     /pseudo
     CDS             complement(5937..6719)
                     /gene="istB"
                     /locus_tag="OO135_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
     gene            complement(6716..7727)
                     /gene="istA"
                     /locus_tag="OO135_003434"
                     /pseudo
     CDS             complement(6716..7727)
                     /gene="istA"
                     /locus_tag="OO135_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         2040 a         1839 c         1901 g         2012 t
ORIGIN      
        1 gaaaggctgg gccatactcc tccggcagaa gcagaaaaag cttattatgc ttccatcgga
       61 aacgatgatc tggcagcctg ggttcacaga taaaacactc tccaggaaac ccggggcggt
      121 tcagttgctg aactgctgga accgctgtat gacatcctgc gccagtatgt gctgatgccc
      181 ggtaaagtcc ataccgatga tatccccgtt ccggtccagg agccgggcag cggtaaaaca
      241 aggactgccc ggctgtgggt ctacgttcgt gatgaccgta acgccgcttc ggaaattgca
      301 gcataagtta tccatgctct gaggtgggtg gaggtgggcc ctccggtcag ggtttacagg
      361 agcactgacc aaacgacccg gtgctgcttc attctaagga atgaggtggt gagcgactcg
      421 ggaaaaggca gaggtctact gtcacgtggg gttcgggaag ctgaacgaaa gtaaactgtc
      481 cgatgaagca tcgaaatctt agatgatgtc aaaatggggc cgtacagccg tcccctgata
      541 agcgcagaag acacctgctt actgactgcg cggcatccgg tgtgaaggca gcaggatccc
      601 gggccagacc atctcaggga acgtgagaac ctgtttccgg cacgtatcgg gaaacagggg
      661 cggccccatt cgtagtagtg atgaaagatc tgtaatgggt ctggagcgaa gggatgggga
      721 tcacattcat tgcagaagac acaacccaaa aggggatgac gtaactgatg caacgaaaat
      781 catttgaaat accaaaagca ctggtctggg catcctatct ggatgtgcgt cgcaacaagg
      841 gagccccccg gatgcgacgg gcagactttg aaaatgttcg accagcaacg tgatggcaat
      901 ctatacaaga tctggaaccg gctatgttcg ggaacatggt ttccgccacc agtgctggaa
      961 aaacggatcc cgaagtcgaa tggcaaggaa cgcattctgg ggatcccaac agtatccgat
     1021 cgaattgctc agggagcgat aaaacttttc atggaagaaa agcttgaccc gattttccat
     1081 gctgattcat atggttaccg accaggcaaa tcagctcatg acgctctgaa acaatgtgcc
     1141 atccggtgct ggcgttatag ctggatactg gaagtggaca tcagtgcgtt tttcgaccat
     1201 gtgaaacatg atctggtgct caaagctctg gaacaccacg ggatgcctaa atgggtcatc
     1261 ctgtactgtc gacgctggat ggaagcgcca atgcagagtt gtgaaaatgg agaattaata
     1321 acccgaacac gaggcactcc gcagggcggg gtcattagtc cgctacttgc taacttgttc
     1381 cttcactatg catttgattt gtggatggaa agagaatatc ggggggtacc gtttgagagg
     1441 tacgctgacg atattgtagt acattgttca cgaatgagtg atgcaacaag gctaaagaac
     1501 aggttatcgg agcgcttctc ggaagtcggg ctggtcctga atgcagggaa aacgaatatc
     1561 gcctacattg acacgtttaa aaggagaaac gtcgcaacga gtttcacctt tctcggatat
     1621 gacttcaaag tgcgtacgct gaagaatttc aaaggcgaac ggtaccgaaa atgcatgccg
     1681 ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa tcaagaagtg gcgtatacat
     1741 cgctcaacag ctgagagttt gctggatttt gcgaggcgct acaatgcgat agtgagaggc
     1801 tggatcgagt actacggaaa gttctggtcc agaaatttca actatcgact gtggagtgca
     1861 atgcagtcac gtctgctcaa gtggatgcag tctaaataca gactttcgaa ccggaaggct
     1921 cagcgaaagc tgacgctggt aaggaaggag tatccgaagc tatttgttca ctggtattta
     1981 ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg agagtgtcac gtacggttcc
     2041 gtgagcagcc ggggagtgaa attctctccg gctgactcga ctcgcgggtt ccgacagcga
     2101 tgacgaacat gcggcagtgt tgtactcgct gatcggcaca tgccgtctga acaatgtgga
     2161 accagaaaaa tggctgcgtt acgtcattga gcatatccag gactggccgg caaaccgggt
     2221 acgtgatctg ttgccctgga aagttgatct gagctctcag taaatatcaa tacggttctg
     2281 acgagccgct tacttttggc gtaattctgc tgcgccttta ctgaacacaa tgccgtattc
     2341 agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa ccaaccgacg
     2401 catattcaaa aaagtctgga cgtcctgttg ctccagagtt tttattggta cgggtcgtat
     2461 agttccatgt tggcaggcct cagctattgc ctgtgcgtcg ttataatcat ttttctggca
     2521 ccgctgataa gctttaacat gttgtgccgg aagcaatatt acatcatggt cgagtttcct
     2581 gatttcccgg gcccagtaat gagatgcccc gccagcctcc atcgcaacag tcattttagg
     2641 ctgttgcacc agccagttaa gcagttgatt gcgagaaagt ttggtctttc ctgcgggttt
     2701 gccatgagca ttgatactga aaagataaaa gttcgtttta gccagatcaa tgccggtgat
     2761 tttctgttca gacatggtat cccgcttact tttgtggtta aagtttatcc atcatggcgc
     2821 aattgaaggc tgccgtgaaa gagggggaat actatctcat cggttactgt taaacggcta
     2881 agagaaacta tctttttcaa gcaaagaaga tacggtgaaa caggcgattt caccgtgaaa
     2941 tactgttcaa aaaaaacgct aataaaagca aaatttcttt taaaacagcc aattaaaaac
     3001 tggtgagttg ctggcgaaaa acttgaattt tcacagcgaa ttcagattat cttttagtac
     3061 aagagcaatc atacgatcta acttatcctg aacttccaac ggtaatctgc cgaattcata
     3121 tgagaacgta cgtcccttta ctcgttttcg agcaaattta tctttactat caaatttcca
     3181 taacaaagtt acaaccgctt tatcctttac tcctgaatca agcaatgaag acatttcctt
     3241 ggttataagc cgcataatac tgtttttaac ctcgtcttca gaaagattgt catcagcagt
     3301 aataagctca atactcggcg agatattctt cataacttca tcaatagata tatcacccag
     3361 cctcagtcgt tcacttaatt cagccagttg cctgtaatcg gcgtatgtca gctcagactg
     3421 aacaggaaaa agagacacaa aatctttagg tacggacgct gcttggaggg cacgagtcac
     3481 cttagctgcc gacaatccct ctctttccgc aatttgcttc tggttcatac cggcctcttt
     3541 gagacgtact aaacgcaacc caatttcacg aatattatgt tcaagtgacg tctgcaaatc
     3601 tttcgccaga tgctgagctt cagaaacagt aagctcatca tccgttacaa gtacacgtaa
     3661 tccaaccttg catagcaaag ctgctgcccg acgacgggaa ccatccaaaa tctcaataag
     3721 atcgccagtc cgaatcccaa tacatggata gaattgttgt aaacgtatag ttctggtgat
     3781 gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg ccattcacct cttcaaccac
     3841 aaaagtttga gtgtccactt tgtcatgatc aaccttgatc tcagtaaatt ttgcctttcg
     3901 gccagtattg agggtaaata catgaactgg agccgatatt tcttcagtat tattcaggat
     3961 atttgtattc agcgtacggc caatagtcgg tcggtgcttt ctgttttcca tcagtagtta
     4021 gcccttataa attcaatacg atcaaaaact gctttagcga aatcttctgc tgccatacgt
     4081 gcatttttca aagcttcacc actgccaaca taagtaacag gattcgcaga tatcacggta
     4141 tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg gcattgaaac atcaagcatg
     4201 tcaccaccga aaatctcttt tgtcaggcta tggcagtatt tatgatctga cttattcgcc
     4261 agtttcgaca tgaagccaat atttgcctgc aaacggcatg aacatccatc ctgttcaata
     4321 atctgtacaa gttcaggtaa acgagccaaa tatttaagcg tggaatggaa atcaacttgt
     4381 gctggcggca ccggtgtaaa catgatatct gcggcagcaa tggcattttt caaaaatgcg
     4441 tccagatgcg ggcctgtatc aataaggatg aagtcgaaat catgcttcag cttatcaata
     4501 atattctcac gcaatatcgc gtgtttgttt tgtcctaata gatgctcctc gcacagagta
     4561 tcccagtttg atgcaataaa ggcgtcgtca atagaagccg gcataacata aacgccaggg
     4621 ataactgacg gaacgataaa atcttccagt aattcctcac gactcacgtt ctgcaacata
     4681 gcctgcggtg cggttgtatc aaccagcccc accgcatgca agtagttcag aaacatagtg
     4741 gctgatgact ggggatctaa atcaaggagc agaatacgta aatcttctga cagcaggtga
     4801 ggatgagcac gcagggcgtg agctacggaa acactactca cggttttaga cacaccgcct
     4861 tttagactac cgatgaatat ggaatatgcc tcactataac gatcgcgata tttaggaatg
     4921 ccacgatgtg catagatatc gataatgttc tggatggtta atgcatattt tgtcgcggtt
     4981 cctgctgggc gtttttcaaa ctgatagccc tgagcctcca tctcaccgac tgcaccatcc
     5041 acgcttgcgc gcgttaattt tggaagctta gctacagcag actttgaata gacttgataa
     5101 aaaacatcag cctgaaactc ctgcttttgc tcctgaatct gttttgttag ggcgagcaac
     5161 attttatctg cacgctgcgc aaccttactt aactgctcaa aactagtcat tccccctccg
     5221 tgctatgtag gattttaact tattacaaaa aaaactgcat tagcagggta atgcatgcaa
     5281 gtgcatattg tacatgctta tattaaaaat gcgcgcattg ctgatttatt aatcatcgga
     5341 atgaatatta ctgtaagata gtatgccttt ccgggagaaa gacgctgatt tcccccgaat
     5401 aatccagttc accaccacac tccggacaac agctttcttc aggctccaga tggtgtattt
     5461 cgcgggaaac atgttccggt aacggacgac gatgacgtga ctgtcgcaac aggcgggggt
     5521 agtgcttcca atcagtagaa catgtgcttt tcaatgaagg agccgatgac tttttcgtgg
     5581 atttccacgg aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat gcgggtgcgg
     5641 agggtccggt tgttacgttc aatacgttgc gtgaagattt tgccggtcag gtgtttctcc
     5701 ttcggcactt ctctggcgta gctgccccag tcatcgctgg tcaccatgcc gatacagaac
     5761 ggagtcaaca gggctaacag ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa
     5821 gccaacacac cgccagtttt agtgttgtac gcataccaga gccagtgttg tgtcaacgac
     5881 ggatgaaaag tgatccactt atatctccac caacggccca atattgatcc accgttttac
     5941 tcaggattag cttctgctat aaccccggcc tttcgtttct gtctgagtcg atagctttct
     6001 cctttgattt gaacgacatg tgagtggtgt aagatacggc ccagcatcgc tgaggtcagt
     6061 gctgcatcac cggcgaacgt ttgatcccac tgcccgaacg gtagattgga tgtcaggatc
     6121 attgcgctct tttcataacg tttagcgatg acctggaaga acagttttgc ttctttctga
     6181 ctgaacggca gatagcctat ttcatcaatg atgagcaggc ggggggccat tactccacgc
     6241 tgaagcgtcg ttttataacg gccctgacgt tgtgccatag ataactgaag taacagatct
     6301 gctgctgttg tgaagcgaac ttggatacct gcacggactg cttcatagcc catcgctatt
     6361 gccagatggg ttttccccac acctgatggc cccagtaata cgatattttc attacgttat
     6421 atgaagctga gtgagcgtaa cgactggagt tgcttctgcg gtgctccggt ggcgaatgtg
     6481 aagtcatact cttcgaacgt tttcaccgcc gggaaggctg ccattcgggt atacatcgcc
     6541 tgtttacgtt gatgacgtgc cagtttttct tcatgaagca gatgctccag gaagtccata
     6601 taactccatg cctggtctac tgcctgttgt gacagcgcag gcgctgcgct tataaggctt
     6661 tccagttgca actgcccggc gagcgccatc agtcgttgat gttgcagttc catcatcacg
     6721 ccactcctct gcagaatgag tcgtagatgg agagtggatg atgctggggg ggttgtcgaa
     6781 gctcaccaga ttttcaccag gatgcacgtc atactctttt ttctccggag gcagtgccag
     6841 catggactgc tgctcttcga gccagcgatc acagggacgg gcctggattg tttcatgctt
     6901 tcgttggtta gcgacatcgt gcagccagcg cagaccgtgg cggttggctg tttcaacatc
     6961 gacagtgctc cccatcgggc gcaggcgagt cattagtggg atgtaaaaac tgttacgggt
     7021 gtactgcacc atccgttcca ccttaccttt agtctgtgcc ctgaaggggc gacacagtcg
     7081 gggatctcct tgccgaactg ccacagcgaa ggatggaacc ggtgctgacc gatctgatat
     7141 gcgtcacgtt gcagaaccac agttttcata ttgtcataca acacttcgcg cggcacacca
     7201 ccaaagaagc ggaacgcatt acgatggcag gtctccagcg tgtcataacg catattgtca
     7261 gtgaattcga tgtacaacat tcggctgtat ccgggaacag caacgaacac gtgaagcggt
     7321 gagcgaccat tacgcatagt gccccagtca acctgcatct gtcgtccggg ttcagtttcg
     7381 aaccgaacgg caggctcctg ctcctgagga accgagagag aacgaatgaa tgccctgaga
     7441 atggtcattc cgccacgata tccctggtct ctgatctcgc gagcgattac cgttgccggg
     7501 attttgtaag gatgagcatc ggcgatgcgt tgacgaatat aatcccggta ttcatccagg
     7561 agtgaagcaa cagcaggtcg cggcgtatat tttggcggct cagattttgc ctgcaaataa
     7621 cgtttaacgg tattgcggga gagccccagt tctctggcaa tcgcccggct actcattccc
     7681 tgcttgtgca ggattttaat ttccataact gtctcaaaag tgaccataag ctctcctgaa
     7741 tcaggagagc agattacccc ctggatctga tttcaggcgt tgggtgtgga tc
//
LOCUS       DAJTMK010000173         7747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTMK010000173 DAJTMK010000000
VERSION     DAJTMK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.177"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..792)
                     /locus_tag="OO135_003435"
     CDS             complement(<1..792)
                     /locus_tag="OO135_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HCR5399151.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPFAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFCEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIISGILIY"
     gene            797..872
                     /locus_tag="OO135_003436"
     tRNA            797..872
                     /locus_tag="OO135_003436"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:830..832,aa:Asn,seq:gtt)
     gene            complement(910..1860)
                     /gene="cbl"
                     /locus_tag="OO135_003437"
     CDS             complement(910..1860)
                     /gene="cbl"
                     /locus_tag="OO135_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5399152.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGISRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKSFRELFPEVRLELIQGTPQEIATLLHNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1962..2879)
                     /gene="nac"
                     /locus_tag="OO135_003438"
     CDS             complement(1962..2879)
                     /gene="nac"
                     /locus_tag="OO135_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5399153.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3206..3281
                     /locus_tag="OO135_003439"
     tRNA            3206..3281
                     /locus_tag="OO135_003439"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3239..3241,aa:Asn,seq:gtt)
     gene            complement(3338..4273)
                     /gene="ldtA"
                     /locus_tag="OO135_003440"
     CDS             complement(3338..4273)
                     /gene="ldtA"
                     /locus_tag="OO135_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5399154.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVKRKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4335..5414)
                     /gene="cobT"
                     /locus_tag="OO135_003441"
     CDS             complement(4335..5414)
                     /gene="cobT"
                     /locus_tag="OO135_003441"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5399155.1"
                     /translation="MQILADLLNTIPAIDSAAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AEQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     QELAKNGVTLFGVGELGMVNTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGIDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSVEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5426..6169)
                     /gene="cobS"
                     /locus_tag="OO135_003442"
     CDS             complement(5426..6169)
                     /gene="cobS"
                     /locus_tag="OO135_003442"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5399156.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGFADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGESILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6166..6711)
                     /gene="cobU"
                     /locus_tag="OO135_003443"
     CDS             complement(6166..6711)
                     /gene="cobU"
                     /locus_tag="OO135_003443"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5399157.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQILYIATSQILDDEMAARI
                     EHHQQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
BASE COUNT         2131 a         2040 c         1662 g         1914 t
ORIGIN      
        1 gtaaatcaat atgccgctaa taatattaag aatattcagg ctaccgttaa tcagtagcgg
       61 tattttcgta ttccctgcac cacgaagtgc cccgctacca attagagtga tggcagctgc
      121 tggataactg agtaccgtca gctccagata agtcaacgcc agtgctttaa cttctgtcgt
      181 ggcatcaccc gcgacgaaat caataatttg ctcgcaaaaa tgatgaataa gcgttgccaa
      241 cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc tcgctcgtcg
      301 tcgatcccgc ttaccgagac taaatgccac aacgacagta gtaccaagat cgatagcagc
      361 aaaaaaagcc ataatgacca tattgaagct gtccgccaat cccacgccgg ccatcgcatc
      421 ttttcccagc cagctgacca gaaaagtgct cagaaccccc atcaacagga cacaggcatt
      481 ctccatgaag ataggaacag caaacggggt tatctcgcgc cagaacaaca ctttgtagct
      541 cttgcgttta gcgtgccagc gagtgccgtg aacaacctgg cgtaaagcag aggagatatt
      601 caaagccgac cttaattgca gaaagtgaaa ccacatttca aataatgagg gagaatcagc
      661 aaagctgcaa agattttcgc caacaaattg tctgcaaatg caacaaactg ttgatagaaa
      721 cggcaaacag ttgggaaaat taaaaatcgg gtttgacaaa agatttttcg ccgttaagat
      781 gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg ttaatccgta
      841 tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc ttttatagcg
      901 ggctttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca cctgccgctt
      961 gatatcctca actgaaagtc ctgcattaca aagttccaga aagcgccaga catagttacg
     1021 ctgaagttgt cctcgcttca gtcccaacca gacagtatta gcatcaaaaa gatgccgcgt
     1081 atccaggcgg attaaattct tttcctcttg ttcaccgctg gattgttcgg caactattcc
     1141 gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgcg cacttaatac
     1201 aatatctgcc agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc gccccgtaat
     1261 cccctgtcgg taagtgatta acggccactt cgctattgat tccagcgtca atggtgtaat
     1321 ttgcgtcaag ggatgatcga gtggaacaag caaactatgg tgccaacgaa accacgggaa
     1381 ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat cagcttcacc
     1441 attatgcaac aacgtcgcaa tttcctgtgg cgtcccctgg attagctcga gccgaacctc
     1501 cgggaaaagt tcgcgaaaag atttaatgac ctctggcaaa ctataacgtg cctgagtatg
     1561 cgtcgttgca atagtgagaa cgccagacgt atcgttggta aacaggtctg caagccgacg
     1621 aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt tgcccggttc
     1681 agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgatgccaa gttcatcctc
     1741 cagttcccga atatgacggc tgatgccgga ctgtgaggta aaaagcatat tcgcaacctc
     1801 tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt ggaaattcac
     1861 ggtaaactcc gggcagttca gattttccgt tattgttaaa gtctaatgcc cggcataaca
     1921 aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac caattgccac
     1981 tgcctttttt ccatcactgg agaactaatc actgacatta acaactcttt cactgcctgt
     2041 gcctgtggtg acaagtttgc tctggcgggt aaatttaagg acaaagagag actcattgaa
     2101 ggagtggtaa tgcgtgacat ccacccattt actgcgccac ataacgaacg cgcagccgat
     2161 tcaggtaata ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt ggcaatagac
     2221 tcaatttcac caatgacttt tgccgtgagc cgccgtaggg aaaaagcctc atcaacacga
     2281 agtctaacag cgctgtaatc actggggaga aaaaggttca tttgcgcaat agcattaaca
     2341 tcaacgcttt gccccgggca atcttgagtt cctaccagga aaagatcttc tttcagcaaa
     2401 gcctgactgg ataccccagc cactggggaa tgctcataaa tcaccgccat atcgagttgg
     2461 tgatttatca gtttttcgtt aagcactgca ccactatttt catgaagata gataacgacc
     2521 tccgggaatt cagcgcgaac cgcctgtaat aagggcatgg tgatggatga cgcagcggtt
     2581 cctggcgcaa agccaatcga gacttgcccc gataatgact gaccaacgtt atgcaccgcc
     2641 agttgggcct gctcacactg acgtaaaatg gcccgtgcat gggtatagag aatttttccg
     2701 gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa gctgttgatt taactcacct
     2761 tccagtgtgg caacctgctg gctgagcgca ggttgtgcga tatgcaatac ttcagcagcc
     2821 tgggtcaggc taccaatatc tacaattttt acgaagtatt tcaggcgtct gaagttcatg
     2881 ttgcctccgg tttttaagaa tcggcccaag tgccgccatt acttacaacc agaaagcaag
     2941 atgcttgcca gttctatttt ggcgttgatg cgcaagctaa ccaactgtca aataaaagat
     3001 tatgatagat tcgtcattgg ccctttttat cagcagtcgc attcacctgc cctataaaag
     3061 gagggtatgc accacgatgg ttcattacac aataagattg aaagctcacc actttgttga
     3121 aattggcagc aaacaaacaa aaaaatacat ttcacccttt gacatcacca tgcactgcca
     3181 ttaatatgcg ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt
     3241 taatccgtat gtcactggtt cgagtccagt cagaggagcc aaattcaaaa aaagcctgct
     3301 ttcgagcagg ctttttactt tctaattacc aacgctctta aaacatctgt cttgaaccag
     3361 aactaatttg cacaggcatt cccgatcgac gttgcaacgc agcatttgcg cgatttacat
     3421 caacttcttg cccgttgata aacgcccgca aagatggggt taccggcaat ggcacttttc
     3481 ggtcagactc atattctgca cgattgcgcg acagcggctc atgaacttcc agccactttg
     3541 aaccatccgg ttccgttgtg tatttcactg gctgatcaat aatttgtaca cgagtgccta
     3601 caggaacatt atcaaacaga tatttgatat cgtcattgcg cagacgaata cagccctgac
     3661 ttacccggag cccaatacca aaattggcat tggtaccgtg gatggcatac aacctgccaa
     3721 tataaatcgc gtacagcccc atggggttat cgggccccgc aggaacaaat gcgggcaaac
     3781 tctcccctcg tttcgcatat tcgcgccggg tgttcggcgt tggcgtccag gttggtgctt
     3841 cttgtttacg tttaacggta gtcacccagt tacgcggggt ttctcgccca gcctggccga
     3901 taccaatagg aaagacttcc actgtgttac tgtctggcgg atagtaataa agacgcattt
     3961 cagcgacgtt aaccacaatc cctttacgaa cagtggcggg caaaatcagt tgctgcggaa
     4021 tggtgagttg tgagccaggc ttcggcaaaa aaacatcagc gcccggattc gcttccagca
     4081 tattacttaa cccttgcccg tattgtgcgg caaaagtctc cagcggctga gtattgtgat
     4141 caggaacagt tacagtaagc gactgcccca ctaaacggct accctctgga ggtaatggat
     4201 aagttaccgc caggctattt tggctggcaa aaagcagagc aaatgagcaa agaatattta
     4261 cacgacgcat catgtccctt tcctaagtcg cgaatactat ccgataagta tagcttttat
     4321 cagacttttc gtttttagct gttcaaatca gaagtcgtat tccccggtag aacaatatta
     4381 ctggcagcaa gttcgcccat gttgttgtat atcgcacaag cagcttcgat gatgggcatc
     4441 gccagagctg caccactccc ctcacctaaa cgcatatcca tattgagata aggctccagc
     4501 cccaaatgcg agagcgctat acgcgcgcct ttttctaccg acaagtgaga aggaatgaga
     4561 tacggtttga ttgcaggaga catctggcag gctgcgagcg cagcagcata agaaagaaat
     4621 ccatccagca acacgggtaa accgcaggaa gcagcaccta acatcactcc agctattccg
     4681 accaaatcaa atccacccac ttttgccagg acatcaatac catcctgagg atttggttga
     4741 ttcaacgtaa tcgcccgacg cacaacatca attttattag ccagtttatc tgtcggcagg
     4801 tttgcgccaa tcccaaccac ttcttcagga tcccggccag tgattgtgct gactattgct
     4861 gctgccggtg tcgtgtttac catccccagt tcacctacac caaacagcgt gacaccgttt
     4921 tttgccagct cctgcgtata acatatgacg tccaaaagca acttttcagc ctgacggcga
     4981 ctcattgccg gagctgaagc aatattgccg ctacctcgtg cgacacgcat gttgataagc
     5041 ccggggatag gctcagcagt atcaatacca acatcaatta cgtggacgtt agcgcccgct
     5101 tgttctgcca gcacacacac gccggttgtt ccacgggtca tattttcagc ctgtatggct
     5161 gtcacttctt ttggagaaat agcgacccct tcctcccaga cgccgtgatc ggcacacata
     5221 accagtaccg cttttttgcc cacatgcggt atgccattca accccggcat tcctgccagt
     5281 tgtatggcaa gcgcctccag ctttcccagg ctaccaacag gtttgagtaa cccgtcaata
     5341 tgccgttgtg cacgcgacat agcggcagaa tcgatggctg ggatcgtatt cagtaaatcg
     5401 gcaagtattt gcatgtcacg tctctttata gcagtgccag caggaagacc agttcaccaa
     5461 gctctatggc tgctcccagc gtatcgcccg tttgcccgcc cagcgtacgt ttgagaagct
     5521 gaccgaggat gaaaatcgcc accatcgtca ccaccatagc agccacacca tgcatacccg
     5581 gcaataatac ggcagcaaaa atagcggcta agccgagtgt gacgcaggtt tgtcgcccat
     5641 caattttgcc aatgaacaca ttgccaagcc cttcttcccg cgcataacga tgacgataca
     5701 tcaataatgc ggcagtcccg cggctgaccg cacatgctgc cgccagcgat gcaaggatcg
     5761 attcgccacg cagagccaac tcgcttaaca ccagaatctt tgccagtacc acaaaaatca
     5821 atgccagacc gccgtgggtg cctaaacggc tatcacgcat gatttccaac atgcgatcgc
     5881 ggctacgtgc agaaaatacg ccatcacagg tatcagcgaa gccgtccaga tggaatcccc
     5941 cggtcatcag cgccagcaca agtacgctaa acagtgccgc cagtggtacg ccacaccatg
     6001 cctgcagcac catgaagacc agcccgctaa tcgcgccaag caataatcca attaaaggaa
     6061 aagtaataat accgcgagaa taatgctcga aatccagtcc ctgggaccag cgacgcggta
     6121 caggcagacg cgtaataaat gagagcatcg cccaaaataa tttactcatt tgatttttac
     6181 tccaataccc gaaaccacca gccatacttc atttgccgca gcggccagct gctgatttac
     6241 ccgcccggca atatcacgaa aatgtcgtgc cagacgactc tccggcacaa tccccattcc
     6301 cacttcgtta gtcactaata caacctttgc ggggcaacgt tggcaggcag caatcaacga
     6361 ctgaatctca gcattaatcg cctgttccat cgcctgataa tcccattcat cagggtcttt
     6421 atcgccgcca taatcaaaca acagattagt caccattgtg gtaacgcatt ccagcaacac
     6481 aacctcattc gggttaatgt ctgcatgaat taattcatca agatgttgcc agcgctccac
     6541 tgtgcgccag tgctccgggc ggccttgctg atgatgttct atccgtgcag ccatctcatc
     6601 atcaaggatt tgcgaggtag cgatatacag aatctgtgaa gagtccccaa taagcgcctc
     6661 tgcgtggcga ctcttcccgc tccgtgcgcc ccccgtcacc aaaatcatca taccgactcc
     6721 tgattcaaat gtatgatggt attcattgaa cagaacaatg gatcccccac cgttgctgac
     6781 cgcaaaacat ttacgcgatt aatactgcgc gtaatataat taaaaatcca gcattctcaa
     6841 tccattacga aataagatgg cattacggaa tagccagact ttctcgtgtt aacgtccgac
     6901 aaaatgcgtc attgacagcg gtttctgtgg attgacacat ttcattcaga taatgaatta
     6961 atgctactgc cggaccaacc agcaaacggg cattgtgttc tgaaatccag gctattgatt
     7021 caacgtcagc gacagatctg cgctgtaaaa gaacttgtaa aacaacgcaa tagaattatc
     7081 cggaatggtg gcgactatgc actagaacac cacaatttcg ctctctcggt aaggcactgt
     7141 cacagcattc aacagaatgt gacttgccag atttattagc gccaccagat gtttaaccgg
     7201 gtaaccacga cccagtcggg gatatattcc atcttctcgc tgacatcatc aagaataaaa
     7261 aggtttatca cactcagagc aacgttcttc agcaggcggg ataattcttc aacatttaga
     7321 atatgtgtta ttaaaggcat acaaactgga cgtattgtat tttcttttgg tggttgacct
     7381 aaggtagcag tttatcctga tgcgctgaga tttctgtaat atctacgtca acattctcct
     7441 cgaatagaaa atgcagcccc ggcaagtgat tcacattttt taacaaaacg ttgttagcgt
     7501 gccattttca gaagctctcc aagaaagcga attcttttct caaattctgc attctcagac
     7561 acagctttct actccatggc aatggcccaa atacgaagtt gctcaggatc attagtggtg
     7621 ctgagaagtt aaataaccat gcggagtacc actcgtcatc acgtatttat atatcatgaa
     7681 gctattgttg tgttttgtac atcagtagat gtattctgat atactccttt tgctagacat
     7741 aaccttt
//
LOCUS       DAJTMK010000174         7740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTMK010000174 DAJTMK010000000
VERSION     DAJTMK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.178"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            564..3977
                     /locus_tag="OO135_003444"
     CDS             564..3977
                     /locus_tag="OO135_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5399158.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRD
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQITRLQKIVEELGVVTDAATV"
     gene            4041..5675
                     /locus_tag="OO135_003445"
     CDS             4041..5675
                     /locus_tag="OO135_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5399159.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5684..6754
                     /locus_tag="OO135_003446"
     CDS             5684..6754
                     /locus_tag="OO135_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5399160.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLMGF"
     gene            complement(6879..7094)
                     /locus_tag="OO135_003447"
     CDS             complement(6879..7094)
                     /locus_tag="OO135_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399161.1"
                     /translation="MGVNELYLAYDFTFFLRMMPYMEPGLIKRDMGELLKILKKHIDE
                     AMNTQVLSSNFCKLVCIYDYIKNSHRY"
     gene            complement(7361..7708)
                     /locus_tag="OO135_003448"
     CDS             complement(7361..7708)
                     /locus_tag="OO135_003448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399162.1"
                     /translation="MFVEWKPELEEADISDSVIHKKIPVKYVQAPSYKEIFEVLSETK
                     KRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEEGVSTTEFVVGYGAISMVKNR
                     NRWQLKDWLDWSV"
BASE COUNT         2258 a         1757 c         1804 g         1921 t
ORIGIN      
        1 tcctgttctc ccaaatatca tggtattgat acgggagatt atgccgtaca ggtacgacac
       61 gttatgtcgt gccaaagggg agtggataga aaaaatggcg aagatgcgaa aagttgatta
      121 atgcattgaa aaaaatgaat taataatgaa cttgggtaaa agcgccatca aaatacgaca
      181 cgacctgtcg tattttgtgt tagccgggaa aagatatctg ctacttacta aaatgattta
      241 gtgctgaaaa tatttataag ccaataaata gacaggtatt acggagtaac gcccaggcta
      301 ttttaacgag cctgctgttt tattggctca aaaaacatat cccatagatg atcagccata
      361 cccgcgttag tgagcaaggt atcccttgag tagccagggc tgaagacgcc cattgatggg
      421 cgaaaaccaa ggttctggcc gttaaagcgg ctatgtcgtt aaacacatca ccaccatatg
      481 ccatccggct cacttcgtcg gccaaattag tgtgatattc tttccagaat ttctgttgta
      541 tccacaacat aaggcgatga ttgatggagc agtctctcaa ttttgaaatg ttgcgcagcc
      601 agtggccgga actggcagaa ctcgcatgta tggcagagcg ctatgttcac tccgatccgg
      661 aaagctgcct ggttaagctg cgcaactaca ccgaattgat ggtgcgctgg ttgtatcgtc
      721 aggagcggtt gccggaaggt attaaggcta atctttacga tttaatgaac gctgatgtct
      781 ttaccagcat gatgccggaa gccatcatca tgaaaatgga tgcgctgcgt atccatggca
      841 accgtgccgc gcacggcgga cgtatcaaag ctaaagatac ttactggctg ctcaaagaag
      901 cgtatttgtt gggaatttgg ctgtatgttc gctacgccca cggtaatgtt gatgactgcc
      961 ccaaatttac actccctcca ttaacacaat cttcaggtcg tgcagatgaa aaacgtctgg
     1021 aagatgcaat caaggctcag gatgaaagcc gtgagcgcga actggcgcta caacgcgcac
     1081 tacagcaaga acaggaaaag gccgaacacc tcactcaacg cctgaatgaa gccagagcgc
     1141 gtaaccagca tgttgccgat attctctcta ttgatgaagc ggaaacccgc cgtcgcctga
     1201 ttgactctcg cttacttgca gccgactgga atgtagggga agaacttaaa aacacggatc
     1261 aagtcacaca agaacatcca gtcaaagagc aacccaccac ctctggtgac ggttatgcag
     1321 actatgtctt gtgggatgag gcacacaaac cgttggcggt ggtggaagca aaaaaaacca
     1381 gcgtcaatgc cgagcaagga cgaattcagg cccggctgta tgcagactgg ctggaaaaag
     1441 aatacggtca gcgtccggtc atcttctaca ccaacggcta tgatatctgg ctgtgggatg
     1501 accataaaac tcatggttat cccccgcgtc gggtgtttgg cttctacagc aaggaaagcc
     1561 tgcaatatct tattcagcag cgtgaaaccc gtctgccgct gaacagagtg ccacacgtaa
     1621 aagataacga aggtaaggcc gttgccggac gtttgtacca gctcgaaacc attgcccgcg
     1681 tcagtgaacg gtttaccaac aagtaccgac aatcgttaat tgttcaggct acgggtacag
     1741 gcaaaacccg cgtggcaatt gcactaagca aactgatgat tgatgcccgc tgggtaaaac
     1801 gcgtcctgtt tctttgcgac cgtaaagaac tgcgtaaaca ggcggcaaat gcctttaatc
     1861 aattcaccaa tgagccgctg tatgtggtcg ggaaatcgaa aaaagcagac aggcagaatg
     1921 ccagaatcta catcgctacc tatcccggta tgatgaaaat catggaccat ttcgatgtcg
     1981 gttatttcga tttgatcatc gccgatgaat cccaccgctc tatctacaac gtctacggcg
     2041 atctgtttaa gtattttgat gccctccaga ttggcctgac ggccacgcca attgacatgg
     2101 tgagtaaaac cactttcggc ctgtttggtt gtgaaggacg tattcctacc gccaactaca
     2161 gcctggaaga tgccatcgcc gataacaatc tggttcccta tgaggttgtc acacacacaa
     2221 cagaattcct gcgcgaaggt atcaaacggg agaaattaag cgacgcacaa atccgcgagc
     2281 tggaagaaca aggcatagat cccaatacgc tggagtttga tggcaaggcg ctggatgagg
     2341 cgatctacaa caaagacacc aaccgctata tcctgcgtaa cctgatggaa aatggcctga
     2401 aagatcggga cggccagttg cctggtaaaa ccatcatttt cgcccgtaac cacaaacacg
     2461 cgctgctgtt gaatgaattg ttcgacgata tgtacccaca gtttgccggg cgcttctgcc
     2521 aggtcatcga caactacgat ccccgcgccg agcaactgat tgatgatttt aaagggctgg
     2581 atgaaagcac caacaaagag ctgaccatcg ctatctccgt tgacatgctc gataccggta
     2641 ttgatgtccc ggaaattgtg aatctggtct ttgccaaacc ggtcaaatca aaagtgaagt
     2701 tctggcagat gattggccgt ggtacgcgcc tctgtccggg gttgtacggt tacgacgata
     2761 acggcaaacc gctggataaa cagaaattcc gcattttcga tcactggggc aattttgagt
     2821 atcacgagct gcataccgaa gaggcggaag tcacagcgac aaaatcgctt gcgcaaaaac
     2881 gcttcgaagc gtgggttatg ctgggggccg ctgcgcagcg taaattcgac aaacaggcgg
     2941 tggatttagt tgctcaccag cttcgcgagc aaatcaacgc actggatgaa aagtcgattg
     3001 ccgtacagga aaaatggcag cagaaagcgc agtacagtga tgaaaaggtg ctgcgccagc
     3061 tttctccgaa aacacagcag gatctgctat ctgttctggc cccgttgatg cagtggctgg
     3121 acgtgcgcgg gcaaagcgat gccatacgct ttgatatgga tattctggcg gcgcaaactg
     3181 cccgttatac caacccggaa gagctggatg ttctctggcc gatcatcgtc gaaaaagtgg
     3241 agcgcctgcc gccgcatttg gcgcaggtgc agcaacaggg acaacgaatc aatcagcttc
     3301 gtgatttaag ctggtggaag caggccagcc tggaagagct ggaggatatt cgcattcatt
     3361 tgcgcggtat catgcacctg atggaaaaag acgcgacgcc taaatttggt tcgatacagg
     3421 tggatattac cgaagatgcg aacctgatcc agacggagac ccgcaaaact aatatccgct
     3481 cgattgattt caaactctat cgccagcagg ttcagggggc gctggagccg ctgttccagc
     3541 aaaacccggt tctgaagaaa atccgcaacg gcgagccagt cacgcaaaac gagctggatg
     3601 agctggcgaa gctggtgctg atccagaacc ccaacgttga tattcgtgcg ttgaaagagt
     3661 tttatccgca ggcaaccgcc agcctggata aacttttgcg taccatcatc gggatggaca
     3721 gcgacgcggt ggaagtgcgt tttgcccagt tcgccgctga taacagcctg accagccaac
     3781 aactgcgctt cctgtcattg ctgaaaaacc acattcgcga ttacggcacc attgaaatgc
     3841 ggcagctctt tgaacagcct tttacgcata tccacaacga aggcgttacc ggtgtgttcc
     3901 ctgatatagc gcaaatcacc cgcctgcaaa agatagtcga agagctgggt gttgtgaccg
     3961 acgcagcgac ggtataatac acgcatgaca agccccggca gcggggcttt tgtatttaaa
     4021 acaggatata aacgctaaca atgattactg gcgacttaaa aagtaaaatc gacggactat
     4081 gggaagattt ctgggtgggt ggtatcacca acccgctgac cgtaatcgaa cagatcacct
     4141 atctgatgta ttcccggatg ctggataccc aggaacaacg cgacgaaaag cgtaaacaaa
     4201 tcgcgggtat tgattttaaa ccgcgttttg cgccagaaca gcaggagttc cgtttcagtc
     4261 actatagcaa ccttggctcg gatgagatga tggaagtggt gcgcgatggc gtattccagc
     4321 atttccgtca gctcggccag gccgatgctt cgaaggtgac gctgctgggc aactttatga
     4381 aagatgctcg tctggagatt gttaagccgt cattgctgac caaagcggtt gaggtaatca
     4441 aaaacctgcc attggatcgc ggcgacacca aaggcgacct ttacgaatac ttgttaagca
     4501 agctgacaac tgccggaatc aacggacagt tccgcacacc gcgccacatt atccgcacga
     4561 tggttgaaat gatggagccg aacccggccc gaggcgagac gatttgcgat cccgcctgtg
     4621 gcaccggtgg ttttctggca accagctatg aatatctgct ggagaagtac agctcgctgg
     4681 aatccattca tactgagatt ggcaccaacg aacgtggcga gctggaagag caaaaaatct
     4741 ttaccggcga tctgctgaca ccgtggcgta accatgtgga taacaacatg ttccacggtt
     4801 acgactttga caccacaatg ctacgtatcg ccgccatgaa cctgattatg cacggcgtgg
     4861 atgcgcctga tatccactat caggacacaa tgagccagag tttcagcaca aacttcccgc
     4921 aggccagtaa aaacgccttc aacctgattc tggcgaaccc gccgtttacc ggttcactgg
     4981 acgaagaaga tatcgactcc acgctgtcgg caatggtgaa aaccaaaaaa accgaactac
     5041 tgttcctggc gcgtattctg caaatgctga aagtgggcgg gcgcagtgcc actatcgtgc
     5101 cgcagggcgt gctgtttggc tctagcaagg cgcaccagtc acttcgcaaa acgctggtgg
     5161 aagataacca actggaagcg gtgatcaatc tgccttctgg tgtatttaaa ccttacgctg
     5221 gcgtggcgac ggcgatcctg atctttacca aaggcggtca aacggatgag gtctggttct
     5281 acgatctaca aaatgacggc tacagcctgg atgataagcg caacccgata aaagacaacg
     5341 atctgccgca tctgctggca agctggaagc attaccgtac tttacgcggg ctaccggttg
     5401 ataactttat gggtaagaag ttagcctcgt tgcttaaaca gcagtacccg gaagggatta
     5461 atgctggcgt tgattttaaa gatcgcacgc aggcggcgtt tgttgtaccg aaagcggata
     5521 ttgctgcgca gaaatacgat ctatccatca accgttataa agaagtcgtg tatcaggcgg
     5581 aggaatatga agatccgaag gtgatattga agcggttaaa ggatctggaa aaagagattc
     5641 tggcggattt ggatgagctg gaggggatgc tgtgagttcg gcgatggtga agttagggga
     5701 tgttattaat gttcactacg gaaaagcatt aaaagctgat cagcgtgtat cgaatggaag
     5761 tgtccatgtt tttggtagtt caggaattgt cggcaaccac gataaaacgc tttgcagcta
     5821 tcccacgata attattggcc gcaaaggcag cgttggtgct attacctggg ctccaagcgg
     5881 aggctggatc attgatactg cttattacgt tgagatcaaa gataataata agttagatct
     5941 ccgatacttg ttttatattc tgagcggaat tgatctcact aagaaaacga ttaccacaag
     6001 tattcctgga ttaaatagag acgatctcta tgatacattt attaaattac caccttttga
     6061 agagcagaaa cgaattgtgg atcttctcga taaagctgag ggtatacgcc aaaaacgtga
     6121 gcaatccatc aaactggctg atgatttttt gcgtgctacg tttgctacaa tgtatgggaa
     6181 tcctattaca aaccccaaaa aatggcctgt ccatcttatg ggagaaatta ttgaatttaa
     6241 aggtggaaat caaccaccta aaagtgattt tatttttgaa cctaaacagg gttatattag
     6301 attagtacaa attagagatt ttaaatccga taaatatgcg acgtacattc cgcaagaaaa
     6361 agcaaaacgt attttcgaag ttgatgatgt tatgattgct cgttatgggc cacctgtgtt
     6421 tcaaatactt agaggtttat ccggcagcta caatgtagct ttaatgaaag cttcacccaa
     6481 agaaaatatt aggaaggggt ttatattcta tcttctacaa cttccggaat atcatgatgt
     6541 tgttgtaaaa aactctgaaa gaacagcagg acaaaccggt gtaaatcttg aactattaaa
     6601 caaattcaat gttcccctcc cacctatata ctaccaagat gagatattag atagattagc
     6661 tagaattgaa aaatttaaag aaaaaataga aatatcatta aatcatttag aaatacaatt
     6721 tttatcacta cagaagagat taatgggttt ttaaatcctt agtatcgaaa taaacattaa
     6781 tttattatat taaattaaga tattttagga aattaaggcg tcactcttag gtaacgcctt
     6841 tttttactat ctcgccccct aagaaggtta tatcttactt aatacctgtg actatttttg
     6901 atataatcat aaatacacac taatttgcaa aaattacttg acaatacctg agtattcata
     6961 gcttcatcta tatgtttttt caatattttg agaagctctc ccatatcgcg cttgattaat
     7021 ccaggctcca tataaggcat cattcttaaa aagaaagtga aatcataagc aagatataac
     7081 tcattgaccc ccaccttgac ctcaggaacg gtttcagagc gtcgtttatc ccatccttga
     7141 gactgaaaac taataggagt gatatttaat cgtgataaaa cgccacctga gagttctgtt
     7201 tcattaatta tactcccatc aggattaatt aatttagcgt gatttaagaa gtgaaaaata
     7261 ggaatgcgcg cattgccttt gcatatattt ggcaaaacat cattaacaac acatccccaa
     7321 tcataatatc cattttcaaa tacaacccct cggatgagat ctacacgctc cagtccaacc
     7381 aatcctttag ttgccatcga ttccgatttt ttaccatcga aatagcacca tatcccacaa
     7441 caaactcagt tgtactgacc ccttcttcaa ttttctctga atccctcaca tataattttc
     7501 ctttcggatc atcagttaag accaactcat aaagttcatc ttttatcatt cggaataaat
     7561 gtgctggaat ccttttttta gtttcactca gcacctcaaa tatttcttta taggatggtg
     7621 cctgaacata ttttaccggg atttttttat gaatcacaga atcactaata tcagcctctt
     7681 caagttcagg tttccattca acaaacaata atttattctg taaaaaatcc aggtaatgac
//
LOCUS       DAJTMK010000175         7728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTMK010000175 DAJTMK010000000
VERSION     DAJTMK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.179"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..731)
                     /gene="pdeC"
                     /locus_tag="OO135_003449"
     CDS             complement(<1..731)
                     /gene="pdeC"
                     /locus_tag="OO135_003449"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5399163.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
     gene            1161..1442
                     /locus_tag="OO135_003450"
     CDS             1161..1442
                     /locus_tag="OO135_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5399164.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(1541..2077)
                     /gene="ssb1"
                     /locus_tag="OO135_003451"
     CDS             complement(1541..2077)
                     /gene="ssb1"
                     /locus_tag="OO135_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5399165.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2331..5153
                     /gene="uvrA"
                     /locus_tag="OO135_003452"
     CDS             2331..5153
                     /gene="uvrA"
                     /locus_tag="OO135_003452"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5399166.1"
                     /translation="MDKIEVRGARTHNLKNINLIIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHAKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5188..5544)
                     /locus_tag="OO135_003453"
     CDS             complement(5188..5544)
                     /locus_tag="OO135_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5399167.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPGRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5548..5964)
                     /locus_tag="OO135_003454"
     CDS             complement(5548..5964)
                     /locus_tag="OO135_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5399168.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6075..6788)
                     /gene="aphA"
                     /locus_tag="OO135_003455"
     CDS             complement(6075..6788)
                     /gene="aphA"
                     /locus_tag="OO135_003455"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5399169.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKSGRNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            7190..7394
                     /locus_tag="OO135_003456"
                     /pseudo
     CDS             7190..7394
                     /locus_tag="OO135_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2006 a         1871 c         1890 g         1961 t
ORIGIN      
        1 ataagatgat tataaaaatg ggttataaga cgttgatatg aagtcgcaac aatggctgca
       61 attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttctttg tacggttaaa
      121 tccttcaaat gaatcagcgg agaaaacgtt gctgccgagg cctctttgct taacgagaaa
      181 aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcggatcatc agacatcact
      241 tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat ataggtcatt
      301 ttatagccag agaaaaaagg cgtatcgcgg taataataga tagaaacgtt aggttcacgc
      361 ttataatcgg ccggtgcaat cgtatagccg tttacaggcg ctatcagcga tgagcataaa
      421 aaatggttat cacgggcata gatcaattca ttaatataaa gatagccacg aataatattc
      481 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
      541 ctcactaaat ctgcctggcg aatgaccagc tcggatttat ccagtgccag ttgagcaaag
      601 gttcggagct gattattcac ttctgatttt gcccaaagga aggcaatcca tagcgaaaga
      661 atgatgggaa agagaactaa aaagataatg cccggcaacg ccagtaattg gtgtcgtgca
      721 cgatgactca ttgctatcaa tttcctgcct gtttataccc ggccattgta aggtgaaaac
      781 cctgggtacc gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg
      841 aaattgatac ctttctaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa
      901 tttcctacag gaatgcagag gcggcgggaa gatatgccct taatgccact gaaaaaccac
      961 ataatctgta cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat
     1021 atatttcaca aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg
     1081 cgatagccag agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct
     1141 cttttcagag gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc
     1201 aacttcttag tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt
     1261 ctgattatgt cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt
     1321 tcgcaatggg gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg
     1381 agtcggtgat gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct
     1441 gatacgtaga agcctccata acggaggctt cttttttatg gctggcagat gctttaatca
     1501 tccaccttaa aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat
     1561 caaagtccat cggcggctcg ttagacggcg ctgccggagc ggactgctgc gggcgagact
     1621 gcgcgccgcc gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct
     1681 gcggctgacc accaccgata ttaccacctg ccggagcgcc accaccctga cgaccaccca
     1741 gcatctgcat ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg
     1801 attgatcggt ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac
     1861 gcagatattc gctggccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag
     1921 tctgttcttt catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa
     1981 tgttggcaac tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac
     2041 caacgagaat aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc
     2101 gttctgaata agtgtaaatg cgcgattgta ccattaccaa tagcgctttt actatgttgt
     2161 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc
     2221 attccaatac tgtatattca ttcaggttaa tttgtgtcat aattaaccgt ttgtgatcgc
     2281 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
     2341 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcatt atcccccgcg
     2401 acaagctcat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
     2461 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
     2521 ttctgtcact gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct
     2581 caattgagca gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa
     2641 tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg
     2701 acgtcccgct ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg
     2761 aaggcaagcg tctgatgcta ctcgcgccaa tcattaaaga gcgcaaaggc gaacacgcca
     2821 aaacgctgga gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct
     2881 gcgatctttc cgatccgccg aaactggaac tgcaaaagaa acacactatt gaagtggtgg
     2941 ttgatcgctt caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg
     3001 cgctggagct ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag
     3061 agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg
     3121 agccacgact gttttcgttt aacaacccgg caggtgcctg cccaacctgt gacggccttg
     3181 gcgtacagca atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg
     3241 gcggtgcgat ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc
     3301 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
     3361 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
     3421 gtggcgatac ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc
     3481 gttataaaga gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc
     3541 gcccatgcgc cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg
     3601 agaatacgcc gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca
     3661 acaatctcaa actagctggt cagcgggcga agattgcaga aaaaatcctt aaagagatcg
     3721 gcgatcgtct gaaattcctc gttaacgtcg gcctgaatta cctgacactt tcccgctcgg
     3781 cagaaacact ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg
     3841 gcctggttgg cgttatgtat gtactggacg agccgtctat cggcctgcac cagcgcgata
     3901 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
     3961 tggagcatga cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg
     4021 cgggtgttca cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatc atggcggtgc
     4081 ctgaatcgtt gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac
     4141 gcgttccggc gaatccagaa aaagtgctga agctgacagg tgcacgcggc aacaacctga
     4201 aggacgtgac gctcacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt
     4261 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
     4321 gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca ggggctggag catttcgata
     4381 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct
     4441 ataccggcgt gtttacacct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
     4501 gtggttatac gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc
     4561 agggcgacgg tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg
     4621 atcagtgcaa aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa
     4681 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc
     4741 cagctctggc gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg
     4801 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
     4861 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
     4921 acttcgccga tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata
     4981 ccattgtggt gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
     5041 tgggaccaga aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg
     5101 tcgcggagtg cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa
     5161 ggccgctttc tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc
     5221 cggtagtaaa ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc
     5281 cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggccagg
     5341 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
     5401 aacagctggg cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat
     5461 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
     5521 ttgtagcaac tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg
     5581 cgatccgccg tggatgcgat gttcccccag ccagatgcct tgccaggtgc cggtctgaat
     5641 acgcccttta tgtaacggca atacaagcga tgttccctgc attgaggatt tgatatgaga
     5701 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg
     5761 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
     5821 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
     5881 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
     5941 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
     6001 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac
     6061 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
     6121 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
     6181 acatcgcgtg cggcggtaat atcattatca gaatcgccat aaaaaattcg gatattttta
     6241 tcctgcagcc attgcgattt tgtatttcgc cctgatttat cgcccgcaaa gatcaccgga
     6301 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
     6361 ttcgtcggac tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
     6421 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
     6481 tcccagaaca caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg
     6541 cgccagaagc ccgggctgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt
     6601 ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc
     6661 tgttcagcaa gcctggcaac gttagtacca gggttaagcg gtgaaggaga tgaggccagg
     6721 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc
     6781 ttgcgcatat ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg
     6841 caaaaaccat ctgaccataa cgccagctgc aggggaaagg aaggattatc tgcggttttt
     6901 gtgatgaaaa gcaggatgat tatatttaac ccagtaaata cgttcctatc tttttttgta
     6961 taaaaaatgt ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc
     7021 ctctgcaaat gagcgaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa
     7081 tgatacagca ttacaaaaac aatcgaagtt tataaagatg attcctgatt gaccagaacc
     7141 ctatcctaca atggtgatta tttttattac tcgcgatgga gatgatgata tgaatatatc
     7201 atatatgaat agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa
     7261 agatttttct tatgtcaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     7321 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     7381 atttatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg cacgctttgc
     7441 tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt atttgatatt
     7501 ggtaatcaat ataaggttaa acttacacaa agcggaaaaa aagagttctc tttaacatta
     7561 gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc aaagcatatt
     7621 cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta aatttaataa
     7681 tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaag
//
LOCUS       DAJTMK010000176         7725 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTMK010000176 DAJTMK010000000
VERSION     DAJTMK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.180"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..2302)
                     /gene="nifJ"
                     /locus_tag="OO135_003457"
     CDS             complement(<1..2302)
                     /gene="nifJ"
                     /locus_tag="OO135_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5399170.1"
                     /translation="MITIDSNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRIIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSPDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKA"
     gene            2576..2842
                     /locus_tag="OO135_003458"
     CDS             2576..2842
                     /locus_tag="OO135_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5399171.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(2839..3261)
                     /gene="hslJ"
                     /locus_tag="OO135_003459"
     CDS             complement(2839..3261)
                     /gene="hslJ"
                     /locus_tag="OO135_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5399172.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(3372..4361)
                     /gene="ldhA"
                     /locus_tag="OO135_003460"
     CDS             complement(3372..4361)
                     /gene="ldhA"
                     /locus_tag="OO135_003460"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5399173.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4569..7208
                     /locus_tag="OO135_003461"
     CDS             4569..7208
                     /locus_tag="OO135_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5399174.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTMAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            7205..7390
                     /locus_tag="OO135_003462"
     CDS             7205..7390
                     /locus_tag="OO135_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5399175.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKDPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            7395..>7725
                     /locus_tag="OO135_003463"
     CDS             7395..>7725
                     /locus_tag="OO135_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5399176.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSIDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKLV"
BASE COUNT         1884 a         1878 c         2057 g         1906 t
ORIGIN      
        1 tggctttaat ctctggattc tgacggtctt tcgccgggca aacttcgacg cacaggttac
       61 aaccggtgca atcttccgga gccacctgca agacatattt ctgcccgcgc atatcacgcg
      121 atttcacatc cggcgaatgc aggctggcag gggcgttttc catcgcttcg ggtggtacca
      181 cttttgcgcg aatagctgag tgtgggcaag cggcgacgca gtggttacat tgggtacaga
      241 gttcctcttt ccagatgggg atctcttcgg cgatattgcg tttttcccag cgagtggtcc
      301 ccatcggcca ggtgccgtct ggcggcagcg ccgaaacggg gagggcatca ccaagccctg
      361 cgagcatcgc tgcggttacg gttttcacga aatcaggggc ggcatcggaa accactggcg
      421 gtcgattggc gctgtgcgga tttaccggtt gcaacggaac ttcttctacg gattcacgcg
      481 ccagcgccag agcctgccag ttgccttcca ccagatcctg gcctttgcta ctgtaacttt
      541 tggcaatcgc accctgcaat tctgcgaggg cgctatcgcc aggcagaatt tgcgtcagat
      601 ggaaaaaagc catctgcatg acggtattaa tacgggccgc caggccacat tcgcgggcga
      661 ttttcgccgc gttaatcaca tagaagcgcg cttttttctg gtttaacacg gcctgaactt
      721 cttgcggcaa gcgcgaccac acttcatctg cgctgtacgg cgtgttgagc aggaaaatac
      781 cgccaggttt taaacgctca gccatctgat atttatcgat aaactgcaac tggtggcagc
      841 caacaaaatc agcctgggaa atgagataag cggaacgaat cggctgttcg ctcacgcgaa
      901 ggtgagaaac cgtcaggccg cccgcttttt tggagtcgta aacaaaatag ccctgtgcgt
      961 accacggcgt ggaattaccg ataatcttga tattgttttt ggtcgcggaa acgctgccat
     1021 cactaccaag gccataaaac aaggcttcaa gtttcgccga gtttggctgg gtgttttccg
     1081 gcaacggcag ggacagattg gttacatcat cgtaaatacc aaccgtaaag cgcgctttcg
     1141 gtttagccgc gttgagctcg gcaaataccg ccagtacaca gtccgggcca aattctttgg
     1201 atgaaagacc atagcgccca ccaatgatac ggggcagagt ttcgcgcttg ccattattaa
     1261 aggcttctgc cagtgcggtc atcacatcca gatagagcgg ttctgcctgg gcaccgggtt
     1321 ctttggttct gtccagtacc gccacgctgc gtacggatcc cggtagagtt tgtagcaagt
     1381 gtttggctga gaaggggcgg tacaggcgaa ctttcagcac gccgactttt tcgccacggg
     1441 ttagcaattc atcaaccact tcttcacagg tgccaatggc tgagcccatc aggataatca
     1501 cccgttcagc ttgcggatgc ccgtaatatt caaacggctg atactgacga cctgtcgcgg
     1561 cagagaaatc attcatcgcc tgttcaacat ggtcatagac tgcgttgtac caggggttgg
     1621 tggcttcgcg agactggaaa taagtgtcag gattggcgga cgtaccacgg atcaccggat
     1681 gttccgggtt gagcgcccgg gcgcgatgag catcaatttc ggcctgcggc atgagatcaa
     1741 gaatcgtgtc atcggccagc gggactattt tattgatttc gtgggacgtg cggaaaccat
     1801 caaagaaatg aataaatggc acgcggcttt tcagcgtcgc gatgtgcgaa atgagagcaa
     1861 agtcttgcgc ttcctggacg tttgccgcac acaacatcgc gcaacccgtc tggcgcaccg
     1921 ccatgacgtc ggaatgatcg ccaaaaatag agagtgcatg tgtggcaacg gtacgtgccg
     1981 ctacatgcag gacaaacggt gtgagttcgc ctgccagttt gtacagtgtc gggatcatca
     2041 gcagcaaacc ctgcgatgac gtaaacgatg ttgaaagggc acccgtctgc aaagcgccat
     2101 gcacggtagc gatagcaccc gcttccgact gcatttcaac cacgcgtggt gtgtctcccc
     2161 aaacgttctt caagccgttt ccggcccagg catcagcctg ttctgccatc gtggaactgg
     2221 gggtaatagg gtagatggcg ataacttcac tggtgcgaaa cgccaccgaa gcaaccgcgc
     2281 cattactgtc aatagtaatc atatgacacc cttacattgc gcaaatgagg ggcgcacgaa
     2341 attgctgcgc gcccagtagt aatctttcaa ttttagcaaa tggctttctt ctgcattttc
     2401 gcttttgtgt cccccacatc agcgtaatga atgttttgat caaacagagg gcaaaaaaat
     2461 agccataaaa aagtaaaaat gcggggcaac gcgcaggaca gctctcgatg tcgccctctg
     2521 tgctgcctat tatttatggc ggtgtcgttt ggcttgaaga tcagaggaag aaaagatgcg
     2581 agcagcgttt tgggtagggt gtgccgcttt attgttgtcg gcgtgtagta gtgaacctgt
     2641 tcagcaggcg actgcggcgc acgtagcgcc aggtttaaaa gcgtcgatgt ccagtagtgg
     2701 agaagcaaat tgtgcaatga tcggcggttc gctttctgtt gcccgtcaac tggatggcac
     2761 ggcgattggg atgtgtgcat tacccaacgg caaacgctgt agcgaacagt cacttgccgc
     2821 cgggagctgt ggcagctatt aattcattaa atccgccagc ttataagtta atgtctgttt
     2881 cgcggtcgcc agcgttaact ggttcgcggt cagatccact tgtgcacctt ctttcagcat
     2941 ttcgctaatg gtgttatcga gttcattaag ctgcgggtta gcgcacatca tacgggtcat
     3001 tgccagccct ttggctgtca gttcaccatt agacagtttg ccttcaccgc taaagcggtt
     3061 acacatgctg ccggaaatca tcattttttc accaaagctg atttctggcg gatttttatc
     3121 gctggtcacg ggcttaccgt ttacgctttc cagcacaaag cgatgatgct gtagctgttc
     3181 tggcgtaaca gcaattttgt cattacttac acatcccgcc atcagcaggc ttagcgcaac
     3241 aaacgcggct actttcttca ttgtggttct caattacagt ttctgactca ggactatttt
     3301 aagaatagag aatgaaaggt cattggggat tatctgaatc agctcccctg gattgcaggg
     3361 gagctgcaac attaaaccag ttcgttcggg caggtttcgc ctttttccag attgcttaag
     3421 ttttgcagcg tagtctgaga aatactggtc agagcttctg ctgtcaggaa tgcctggtgc
     3481 ccggtaaaca gcacgttgtg gcaggcagac aggcgacgga atacgtcatc ctggatcacg
     3541 tcgttggatt tatcttcaaa gaacagatcg cgttcgttct catacacgtc catacccaac
     3601 gaaccaattt tctgattttt cagcgcttca attgctgcct gagaatcaat caatgcaccg
     3661 cgactggtat tgacgatcat cacgccattt ttcatctgat cgaaggcggc ttcgttcaac
     3721 agatggtagt tttccggtgt cagcgggcag tgcagagaga taacgtctga ttcagagaac
     3781 agggttggca gatcgacata ctccacaccg agttccagcg ccgctgcact tggatacgga
     3841 tcgaacgcca gcagacgcat accaaaacct ttcagaatgc gcagcatcgc cacaccgatt
     3901 ttaccggtac cgataacgcc tgccgttttg ccatacatag taaagccggt cagaccttcc
     3961 agagagaagt tagcgtcacg ggtacgctga tacgcgcggt gaatacggcg gttcagcgtc
     4021 atcatcatac cgatggcgtg ttcagcaacg gcctctggat cataggctgg aacacggact
     4081 actttcagcc ccagttcttt tgccgcatca aggtcgacgt tattgaaacc ggcacagcgc
     4141 agggcgatat atttaacgcc gtgctttttc agctcttcca gcaccgggcg gctgccgtca
     4201 tcgtttacga aaatacatac cgcttcgcag ccattggcag ttttagcggt tttttccgtc
     4261 agcagaaagt caaaaaattc cagctcaaag ccaaaggact cgttcacctg ttgcaggtac
     4321 ttcttgtcgt actgttttgt gctataaacg gcgagtttca taagactttc tccagtgatg
     4381 ttgaatcaca tttaagccac taaaaatatt ttacaaaatt tcaaatataa ttgaaaacca
     4441 tggcgatatt gaaaaattca tcaacaacta tgcttagtgt aggcgcaacc ttcaactgaa
     4501 cggttaaaca tgccacaata cccgtattga atgcttaatt tttcgctaaa tcaggatatt
     4561 aactacccat gctgggtaaa tataaagccg ttctcgcgct gttattactg attattcttg
     4621 tgccgttgac gctgctgatg acgctcgggc tgtgggttcc cacgatggcg ggcatctggc
     4681 taccgctcgg gacacgtatt gcattagatg aaagcccacg cattacgcgt aaaggtttaa
     4741 tcattcccga tctccgttat ctggtgggag attgtcagct tgcgcatatc accaacgcca
     4801 gcctttcaca tcccagccgc tggttattga acgtcggcac ggtagaactt gattctgctt
     4861 gtctggcgaa attgccgcag acggagcaat cgccagctgc tccaaaaacc ctcgcgcagt
     4921 ggcagtccat gctgcctaac acctggatca atatcgataa actgattttt tctccctggc
     4981 aggaatggca gggaaaactc tctctcgcat taacctctga tatccagcaa ctgcgttatc
     5041 agggcgaaaa agttaaattt caaggccagc tgaaagggca acaacttaca gtcagcgaac
     5101 tggatgtcat cgcgtttgaa tatcagccgc cggttaaact ggtgggggaa tttactatgc
     5161 cgctcgtgcc ggatggactt cctgtaagtg ggcatgctac tgcgacgtta aacttgccgc
     5221 aggaaccgtc actggtggat gcagagctgg actggcagga aaatagcggg caattgattg
     5281 tgctggcacg tgataacggc gatccgttgc tcgatttgcc gtggcaaatt actcgtcaac
     5341 aattgaccgt aagcgatggt cgctggagtt ggccgtatgc aggttttcct ttgagtggcc
     5401 gactgggtgt caaagtcgac aactggcagg cagggcttga gaacgctctg atcagcggac
     5461 gactgagtgt gctgacccag gggcaagcgg gtaaaggcaa cgcggtgcta aattttggcc
     5521 caggaaaatt aagcatggat aacagccagc tgcctctgca gctgaccggt gaagcgaaac
     5581 aggcggacct cattttatat gcccgcttac ctgcgcagct aagtggaagt ctgactgacc
     5641 caacgctggc ctttgagcca ggcgcgttac ttcgttcaaa gggaagagtc atcgattcgc
     5701 tggacatcga tgaaatccgc tggcctttag cgggtgtaaa agtcacccaa cgtggtgttg
     5761 acggacgttt gcaggccatt ttgcaggcgc atgaaaatga actgggcgat ttcgtgctgc
     5821 atatggatgg gctggcgaat gattttctcc ctgacgcggg ccgctggcag tggcgctact
     5881 ggggaaaagg cagttttaca ccgatgaatg ccacctggga tgtcgcagga aaaggtgaat
     5941 ggcatgacag cacgattacg ctgaccgacc tctccaccgg tttcgaccag ttacaatacg
     6001 gtacgatgac ggtagaaaag ccgcgattaa ttctggacaa gcccgtcgtc tgggtacgtg
     6061 acgcacagca tccctccttc agcggcgcgc tgtcactgga cgccgggcaa acgctgttca
     6121 ctggcggcag tgtgttaccg ccatcaacct taaaatttag cgtcgatggg cgcgatccga
     6181 cgtatttcct ctttaaaggc gatttacatg ctggtgagat tggcccggtt cgggtaaatg
     6241 gtcgctggga cggtattcgt ctgcgcggta acgcctggtg gccaaaacaa tcactgactg
     6301 tattccagcc gctggtgcca cccgactgga agatgaactt acgcgatggt gaactgtatg
     6361 ctcaggttgc attttctgct gcgcctgaac aaggattccg cgcgggaggg cacggcgtgt
     6421 tgaaaggcgg tagtgcctgg atgccagata atcaggttaa cggtgtcgat tttgtcctgc
     6481 ctttccgttt tgccgatgga gcctggcatc tggggactcg cggccccgtt acgttgcgaa
     6541 ttgccgaagt gattaatctg gtgacagcga aaaatattac ggctgatttg caagggcgtt
     6601 atccgtggac tgaagaagaa cccttgctgt tgactgatgt tagcgtcgat gtgttaggcg
     6661 gtaacgtact gatgaaacaa ttacgtatgc cgcaacatga cccggcgctg ttgcggctga
     6721 ataatctctc atccagcgaa ctggttagcg ccgtcaatcc gaaacaattc gccatgtccg
     6781 gggcatttag tggtgcattg ccgttatggc taaacaacga aaaatggata gtgaaagatg
     6841 gttggctggc aaatagcggg ccaatgacct tgcggctgga taaagacacc gcagatgcgg
     6901 tggtgaaaga caatatgact gcgggttcag caattaactg gttgcgctat atggaaatta
     6961 gccgttcatc gacaaatatt aatttagata atctcggttt attaaccatg caggccaaca
     7021 ttacaggtac cagtcgcgtt gatggtaaaa gcggtacggt aaaccttaat taccatcatg
     7081 aagagaatat ttttacgctg tggcgcagtt tacgctttgg cgataatctc caggcatggt
     7141 tggagcagaa cgcacgtctg ccgggaaatg actgtccgca aggaaaagag tgtgaggaaa
     7201 aacaatgaaa attttactgg ctgcgttgac gtcatctttt atgctggctg cctgtacgcc
     7261 gcgtattgaa gtcgctgcgc ccaaggatcc gatcactatc aatatgaacg ttaaaattga
     7321 gcatgagatc atcatcaagg cagacaaaga tgtcgaagag ctgcttgaaa ctcgtagcga
     7381 tctttactga ggcgatgatg aagaaaacat tacttctttg tgcgtttctt gttgggctgg
     7441 taagcagcaa tgtaatggca ttgactctgg atgaagccag aacgcagggg cgggtcgggg
     7501 aaacatttta tggttatctg gttgcgctga aaacggatgc tgaaacagag aaattagtaa
     7561 ccgacattaa tgccgaacgt aaagcgagtt accaacaact ggcaaagcaa aataatgtgt
     7621 ctatagatga tatcgcgaaa ctcgccgggc aaaagctcgt agctcgggcc aaaccggggg
     7681 aatatgtaca agggataaac ggtaaatggg tacgaaaatt ggtaa
//
LOCUS       DAJTMK010000177         7597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTMK010000177 DAJTMK010000000
VERSION     DAJTMK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7597
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.181"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..464)
                     /gene="bglG"
                     /locus_tag="OO135_003464"
     CDS             complement(<1..464)
                     /gene="bglG"
                     /locus_tag="OO135_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5399177.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVG"
     gene            complement(750..1475)
                     /gene="phoU"
                     /locus_tag="OO135_003465"
     CDS             complement(750..1475)
                     /gene="phoU"
                     /locus_tag="OO135_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5399178.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(1490..2263)
                     /gene="pstB"
                     /locus_tag="OO135_003466"
     CDS             complement(1490..2263)
                     /gene="pstB"
                     /locus_tag="OO135_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5399179.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(2354..3244)
                     /gene="pstA"
                     /locus_tag="OO135_003467"
     CDS             complement(2354..3244)
                     /gene="pstA"
                     /locus_tag="OO135_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5399180.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(3244..4203)
                     /gene="pstC"
                     /locus_tag="OO135_003468"
     CDS             complement(3244..4203)
                     /gene="pstC"
                     /locus_tag="OO135_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5399181.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(4290..5330)
                     /gene="pstS"
                     /locus_tag="OO135_003469"
     CDS             complement(4290..5330)
                     /gene="pstS"
                     /locus_tag="OO135_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5399182.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKYNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(5578..6651)
                     /locus_tag="OO135_003470"
     CDS             complement(5578..6651)
                     /locus_tag="OO135_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399183.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANEIKVGTTFNTTITNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYNLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(6662..>7597)
                     /locus_tag="OO135_003471"
     CDS             complement(6662..>7597)
                     /locus_tag="OO135_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5399184.1"
                     /translation="WKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLSG
                     WWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLTY
                     DGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLEI
                     DNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVKA
                     EFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQV
                     TLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
BASE COUNT         1963 a         1947 c         1735 g         1952 t
ORIGIN      
        1 cccacttcat ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct
       61 tcccctagct ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc
      121 agcaacacgt tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata
      181 taaatactgt cctgtaattt tcccaagcgc tcctgcgcta aagagataat acgatcacag
      241 gttgccatca cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca
      301 tgactgctca aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt
      361 ttttgaaagc caattcctcg ccccatgacg actttttccc gctgttgatc atcaataacc
      421 accacaacat tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt
      481 ataaacaaaa aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa
      541 tgcagtaaca atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat
      601 taatttaatt gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc
      661 gcagttatta actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat
      721 aattaagtta ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt
      781 ttatccagct catcaccgcc tacatgacgg aaatcctgcc ccttcacgta gtagaagata
      841 aactcacaaa tattctggca gcggtcgcca atacgttcga tagaacgcgc gcagaacagc
      901 gcagtaagta cgctcggaat ggtacgcgaa tcttccatca tgtaggtcat cagttgacga
      961 acaatacctt cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg
     1021 tcaatgtcca tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg
     1081 cccagcgact ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca
     1141 gtacggcaga ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg
     1201 atcaccataa ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg
     1261 caggcttcat cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc
     1321 ttcgccagat cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc
     1381 accatgccgc ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg
     1441 ccggaaatat gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac
     1501 gaccggtgat gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt
     1561 tgctgaactc aatcaattcg cccaggtaca taaacgccgt gtggtcggaa caacgcgcag
     1621 cctgctgcat gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca
     1681 gctcttcaat acgcccggta gagatagggt cgagcgccga acacggttcg tcgagcagca
     1741 gcacttccgg gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag
     1801 agagagagta accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg
     1861 tcaatgccca ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa
     1921 cgccaaaagc gatgttgtcg tagatggaca tcggaaacgg cgtcggtttc tggaacacca
     1981 tgcccacttt cgcacgcagc agtgcgatat cctgagagtt ggtcaggatg ttgtcgccat
     2041 caagcagaat ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg
     2101 tacgcagcag cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt
     2161 tagcgatatc caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat
     2221 tacgaacctg aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat
     2281 cgtgcctgat gcacttcgct aatcaggctt accgtaggcc ggataaggcg ttcacgccgc
     2341 atccgacaaa gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgct agaatgttca
     2401 gcagcagtac gcacagggta ataatcaata ccccggccca ggccagttgt tgccattccg
     2461 caaacgggct catcgcaaac ttaaagatcg tcaccggcag gttggcgatc ggctgcatca
     2521 tgtccgtgct ccagaactgg ttggagagcg cggtaaacag cagcggcgcg gtttcaccag
     2581 caatacgggc aatcgccagc aggataccgg tcatgatccc ggacaccgac gctttcagcg
     2641 taatcgcaga gatcatcttc cacttcggtg tacccagcgc ataagccgct tcacgcaggc
     2701 tgtccggcac cagtttcagc atgttctcgg tggtgcggat aacaatcggc acctgcaaca
     2761 gcgccagggc aatcacgccc gcccagccag agaagtgctc catctgcgcc accacgatgg
     2821 tgtaaacaaa cagaccaacc acaatcgacg gcgcggagag cagaatgtcg ttaatgaagc
     2881 gaatcacttc tgccagccag gatttacgac catattccgc cagataaatc cccgccataa
     2941 tgcccagcgg cgtaccgaat accgtggccc acaaaattaa cagcccgcta cccgccaggg
     3001 cgttcgctag accaccacct tccgtattgg gcggcggcgt catttcagtg aacagcgcca
     3061 gcgacatacc gtcgataccg cgagtgatgg tggacattaa aatccagatc agccagaaca
     3121 ggccgaaggc catcgtcgcc atcgagagcg tcagcgcaat acggtttttg aggcggcgac
     3181 gcgcctgcat tttgcggcga gattcagcca gcgccgcagt ggtttggatt tcaaccatag
     3241 ccattagcgt gccccctcat tcttagccag gcgcataatc ataaacttcg atgcggcgag
     3301 gacgatgaag gtaatcacaa acaggatcag gcccagttcc atcagtgcgg caacgtgcag
     3361 accggattcc gcttccgcaa attcgttcgc caacgcagag gtgatactat tgcccggcat
     3421 gtacagcgag gcgctgtcga gctggtaggt gttaccgatg ataaaggtca ccgccatggt
     3481 ttcaccgagc gcgcggccca gccccagcat gatgccgccg ataacaccat ttttggtgaa
     3541 cggaagaacg atacgccaga taacttccca ggtggtgcag ccaataccgt aggccgactc
     3601 tttcatcatc accggggttt gttcgaacac atcacgcatt accgccgcaa tgtacggaat
     3661 aatcatgatg gcgaggatca cgcctgccgc gaggataccg ataccaaatg cggggccaga
     3721 gaacagcgcg ccaacaatcg ggatattcga catgatattg ccgaccggct cctgaaagta
     3781 aacggcgaac agcggcgcaa agataaacag gccccacatg ccgtaaacga tacttggaat
     3841 ggctgccagc agctcaatgg cgatacccag cgggcgtttc agccagccag gcgcaagctc
     3901 agtcaggaac agggcgatac cgaaactcac cgggacggcg atcagcagcg cgataaacga
     3961 agtcaccaac gtaccgtaga tcggcaccag cgccccgtag atatcgttcg gtgcatccca
     4021 ctctttggtc cataggaaag ccagaccaaa tttctgaatg ctcggccagg aggagatgat
     4081 cagagagaca ataatgccac ccaacatcaa tagcacaatc agcgccgcca gttttaccag
     4141 cacgctgaaa attatgtcgc cctttttacc cggtgggtta aaagcaggct tggttgcagc
     4201 cataagttac tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc ggatgcggcg
     4261 taaaacaccg tacccggcct ggagttttat tagtacagcg gcttaccgct actgtcttta
     4321 atattggtct tccacgcagc gcgaacctgt tcaactacac tatccggcag gctggcgtaa
     4381 tccaggtcgt tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa tttcagcact
     4441 tctgtgcctt gttctggttt cttctgatct ttgtggatca gaatgaacgt ggtagaggta
     4501 ataggccatg catcttcgcc tttctggttg gtcagatcct gagcgaaggt tttgctccag
     4561 tctgcacctt ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg tttaccatca
     4621 gcggagatca gtttggtgta cgccaggttg ttctgcttcg cgtaagcata ttcaacataa
     4681 ccaattgcac ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc tttaccgccc
     4741 agaccgatcg gccattttac ggtagagcca gtaccaacgt tgtatttcca ctcttcgttc
     4801 actttcgcca ggtagctggt gaagacgaag gaagtcccgg agccatctgc gcggcgtact
     4861 acagcaatgt tttgtgaagg cagtttcaga cccggattca gtttggcgat ggcttcatca
     4921 tcccacttct tgattttgcc caggtagatg tcgccgaggg ttttaccatc cagcaccagt
     4981 tcgccagact tcagccctgg aatgttaacc gccagcacca cgccgccaat cacggtcggg
     5041 aactggaaca gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc agaggcacca
     5101 aaatcaacgg tattagcgat aatctgtttt acgccaccgg aagaaccgat accctggtag
     5161 ttaactttat taccggtttc tttctggtaa gtgtcagccc atttggcata caccggcgca
     5221 gggaaggttg cacctgcacc tgtcaggctt gcttctgcaa acacagagaa agcgctcatc
     5281 gataaggtcg cggcgacaac agttgcgacg gtggtacgca taactttcat aatgtctcct
     5341 gggaggattc ataaagcatt gtttgttggc tacgagaagc aaaataggac aaacaggtga
     5401 cagttatatg taaggaatat gacattttta tgacagaaag gaattaccaa ataacttact
     5461 gattttaaat gtgaagatga aaatcccaca tgtcatcttt ttgtcatgat gcatttaaaa
     5521 gtgataaaga aatgggtgag atgacaacga tgtgatgaac aatcgttgtc agacctatta
     5581 gcgcacaatc tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg ctgggcgagc
     5641 tccagtaagg ctcgcaggag cagctgaaaa agttacacta cctgattttt caccatacac
     5701 tgttgattta cccatgtctg ttggcaactc accaccgttc actttgattt ccttattatt
     5761 tttgtcgtaa atttttatcc ccacgttaga gtttgaagtt acgaccattg aattgtccgt
     5821 attgggttcg gtagcataaa aagacgtact gatatcatcg gtattcagta tccccaggtt
     5881 ttcacactca atagtgaccg ttttagtaat tttatgtgtt gttaccgcac ttccaacaac
     5941 agatgaaaaa tctgcagctg gtatttcttt aaaatcaaaa taaactggct gtaaattatt
     6001 tatttcgcaa ctttgcggag ctgtaatatc gccccgaata taaacatcag ccatcggctg
     6061 tgagtaatcc acaggggtgt cacggctaat tgttccatac aatgtcacta tctttgtttc
     6121 aggaataatc aattttcctg caaatgtctt cttgatataa aaatatacta tcgctttagc
     6181 cccactagat atacttaaag gttcaatgcg gttgcaattg tactttgtat taccagacgg
     6241 gttgttagca tgatattcaa aaggtgcatt cacataccca accccaagaa tatctatagc
     6301 aatcccaaca tcaaggttcg gattaagatt ataataatta atattagagt gaaccccagc
     6361 ataagttaat gcctggttta tttttgacgt ataatagata taggggaatt ctcctacgtt
     6421 accagtatca caatgacagc gtccaggata tgaggatcca ttagttatag tggtgttaaa
     6481 tgtcgttcca accttaattt cgttcgcggt aataagatat cccgagaaat caagcgtact
     6541 gtgaaatacg ccattatcag gggtacagat acctttagga gctgcaagtg ctacactact
     6601 taacgagagc ataaacacag caaagcacca ctgtttgata tacttgttca taaaaatcct
     6661 tttaatgaca tgtcacaccc tgttcaatta atccgctctc actttctggt gtcgaaagtg
     6721 aataattgaa gatgcatttt gttgaggcgt tttctcccca ggataacgta acctgtcctt
     6781 tttctggtag accagaaaga taaagttcgc cacggtcacc gacaattcct gaatctgcca
     6841 aacttgcatc ttgtatcgca gcaatggctc caaaaggtac cggtttacca tttgcattta
     6901 acactctgaa gagaacgcga taacccacat gagtcacaaa ctccgcttta accactgcac
     6961 cacgcgtggg aaccatcgta acgacggtat tatcaagttc tatattcgac ttcgagaaat
     7021 agttatcgct gattgctacc cgattttcat cataagggtt tagctgtgtc ttgaccgtat
     7081 agccgcgcca gtccgtcgca gcaccgcgca tattatctat ttctaacccg gcggccccag
     7141 gtgctttaac aagagcaata gtttcaccta actcctgact taacgttacc ccttcgctgt
     7201 gtgccagaat tccccctctg ataccataat tcagcctctg ggaattttgg ctgtagttat
     7261 agctgccgtt caccgtgcca taagagccat cataggtcag ggacatatta ccgccaacct
     7321 catcatcatg actgttatag gactgcatta aattccatga aaggttattt ctttccagcg
     7381 ccgtaccgct gagtccgagt tgatgtgagc taccggaatt atcgttttgc gaaacagaat
     7441 aggtggcgta actattaccc caccagcccg ataaaggaac agataaagag agcatgtaga
     7501 tctgctcact ggtgttatca ctggcatggt tgttccccgt cccctgtctg gcagtattgg
     7561 aataactcca ggagacgttc cacgagagac gcttcca
//
LOCUS       DAJTMK010000178         7590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTMK010000178 DAJTMK010000000
VERSION     DAJTMK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.182"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1226
                     /locus_tag="OO135_003472"
     CDS             <1..1226
                     /locus_tag="OO135_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5399185.1"
                     /translation="INMMSRLEHVEEEKINYDFFLNLPEINRSKLERIDIRTSQLITP
                     LFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLPSTPYTTDANGRGP
                     AWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLTALKSDATPEVRRE
                     QVAALRQQLNDVTEAHELLRDADALVEKSIWLIGGDGWAYDIGFGGLDHVLSLTENVN
                     ILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMMMYGHVYVAQISLG
                     AQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQLTATGFWPLYRFDP
                     RRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQLWKDAAADLQKRYD
                     FLAQMAGKAEKSNTD"
     gene            1592..2725
                     /gene="ompN"
                     /locus_tag="OO135_003473"
     CDS             1592..2725
                     /gene="ompN"
                     /locus_tag="OO135_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR5399186.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            2866..3300
                     /gene="uspF"
                     /locus_tag="OO135_003474"
     CDS             2866..3300
                     /gene="uspF"
                     /locus_tag="OO135_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5399187.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKQIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            3857..4771
                     /gene="sitA"
                     /locus_tag="OO135_003475"
     CDS             3857..4771
                     /gene="sitA"
                     /locus_tag="OO135_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5399188.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            4771..5598
                     /gene="sitB"
                     /locus_tag="OO135_003476"
     CDS             4771..5598
                     /gene="sitB"
                     /locus_tag="OO135_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5399189.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            5595..6452
                     /gene="sitC"
                     /locus_tag="OO135_003477"
     CDS             5595..6452
                     /gene="sitC"
                     /locus_tag="OO135_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5399190.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRL"
     gene            6449..7306
                     /gene="sitD"
                     /locus_tag="OO135_003478"
     CDS             6449..7306
                     /gene="sitD"
                     /locus_tag="OO135_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5399191.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         1949 a         1793 c         1820 g         2028 t
ORIGIN      
        1 ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga gaaaatcaat tacgatttct
       61 tcctcaacct gccagaaatc aaccgtagca aactggaacg tattgatatt cgtacatcgc
      121 agctgattac accgctgttt gaatattccg gtgcttgctc cggttgtggc gagacgccgt
      181 atattaaatt gctgactcag ctctatggcg accggatgtt gatcgctaac gccaccggct
      241 gttcttcaat ttatggcggt aacctgccct ctacaccgta taccaccgat gccaacggtc
      301 gtgggccggc atgggcgaac tctctatttg aagataatgc cgaatttggc cttggtttcc
      361 gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct gctggatcaa tttgccgata
      421 aaatcccggc ggaattactg acggcgttga aatcagacgc tacgccagag gttcgtcgag
      481 aacaggtcgc agctttacgc cagcaactca acgatgttac cgaagcacat gaactgctac
      541 gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat tggtggtgat ggctgggctt
      601 acgatatcgg ctttggcggt ctggatcatg tattgagttt gacggaaaac gtcaacattc
      661 tggtgctgga tacgcaatgc tattccaaca ccggtggtca ggcgtcgaaa gcgacaccgc
      721 tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa agcgcgtaaa gatcttggcg
      781 tcagtatgat gatgtacggt catgtttatg tggcgcagat ttctctcggc gcgcagctga
      841 accagacggt gaaagcgatt caggaagcgg aagcgtatcc gggaccatcg ctgatcattg
      901 cttatagccc gtgtgaagag catggttacg atctggcact cagccatgac cagatgcgcc
      961 aactcacagc taccggcttc tggccgctat atcgcttcga tcctcgtcgt gccgatgaag
     1021 gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga agcactggaa gaaacgttac
     1081 ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc agaagtggca gaacagttat
     1141 ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt cctggcacaa atggccggaa
     1201 aagcggaaaa aagcaacacc gattaaatgc tctggataag gattatccaa ttctaaaaaa
     1261 aagcccggac gactgttcgg gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa
     1321 tggcccaaca gaaaataaaa tctgtgacaa taaaggcata taacccgcgc agaataacgt
     1381 atagaaaatt aaaagtataa tttttatttt gtatgtatta tcttcgatac attcaccaga
     1441 cttattatca tttcagaaga attatttgat taaggtttta cttaaggcgt aacaaatgat
     1501 atttatcggc taactgaact tctcctttag gatgttttca ctccccggaa tggggataaa
     1561 aggcaaataa aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat
     1621 tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa
     1681 caaattagat ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa
     1741 agatggcgat cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca
     1801 actcactggc tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa
     1861 aaaccagtca tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt
     1921 cgattacgga cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc
     1981 tgaatttggc ggtgactctt ataccaatgc agacaacttt atgactggtc gagccaatgg
     2041 cgtcgcgact tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt
     2101 gcagtatcaa ggtaacaacg aaggagccag taatggtcag gaaggcacca acaacggacg
     2161 tgatgttcgc catgaaaacg gtgacggctg gggtttttcc acaacatatg atttaggcat
     2221 gggctttagc gctggtgcgg catacacctc ttctgaccgc accaatgacc aggttaacca
     2281 tactgcggcg ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa
     2341 caatatttac ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag
     2401 cgattatgct gtggcaaaca aaacccagaa ttttgaagtc actgcccagt accagtttga
     2461 ttttggtctg cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc
     2521 cggtggtgca gacaacccgg caggtgttga tgataaagat ctggttaaat acgccgatgt
     2581 tggcgcgact tactatttca ataaaaacat gtccacctac gttgactata aaatcaacct
     2641 gttggatgaa gatgacagct tctacgctgc caatggcatc tctaccgatg atattgtcgc
     2701 tttaggtctg gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg
     2761 cttattcttc ctcctttgat ctaaattaaa aatgtgaact ccatcattac acaaaaagtg
     2821 tcatctggcg ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt
     2881 cttgtcccta tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag
     2941 gcagaggcaa agattgacga tgcagaggtt catttcctga cggtaatacc ttcactgccc
     3001 tactatgcct ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa
     3061 gcggaagcca aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga
     3121 gtgcatgtcc atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagcag
     3181 atccccgctc atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc
     3241 ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga
     3301 cactaacgcc cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt
     3361 tcccctctaa tccgtaccat acacgccact gtttttatat cagatttctt atgctgggca
     3421 ttccggcatg acgcatactc ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc
     3481 gcaaaatcaa gaaattagta agaaagaaca atacaacctg aacaagtaag ggcaaaaatc
     3541 acaactatct gactcataag tattttattt atttttcaat atgttaaaca ttatcagcga
     3601 caacaataag ctacgataac ccactctttt tttgccccat ttttgccatc aacaattttc
     3661 ctcataaagt catttcaaag cctaatagct gctctacttt tttcctcaat atcatgtcgc
     3721 cagcaggaaa caacctaata cctgttttag gcctcaaagc tgttagtgat aattattttc
     3781 attttgtata ggttatgcta taaaacatag ccgttgctat gtttgttcta attttgctca
     3841 ctataggtac taaattatgc actcaataaa aaaagtaacc atgctcttgg ggtgcctcgc
     3901 cctcacctgc tcgatcgcat ttcaggcaag tgcaactgaa aaattcaagg tcattacaac
     3961 atttaccatc atcgcggata tggccaaaaa cgtggctgga gatgctgcag aagtctcatc
     4021 cataaccaag cctggtgcag aaattcatga gtatcagcct acccctggcg atattaaacg
     4081 tgcacagggg gcacaactga ttctcgccaa tggtatgaat ctggaattgt ggttccaacg
     4141 cttttaccag catctcaatg gggttccaga agtaattgtc tcttcgggtg tgacgccagt
     4201 agggatcacc gaaggaccct atgagggcaa acctaatccc catgcatgga tgtcgccaga
     4261 taatgctctg atttacgtcg ataatattcg tgatgcgttg ataaaatacg atccggcaaa
     4321 tgcacaaacc taccaacgta atgccgatac ttataaagcc aagattaccc aaacccttgc
     4381 ccccctgcgt aagcagatta cggaactccc tgagaatcag cgatggatgg tcaccagtga
     4441 aggggctttt tcttatctcg cacgcgattt ggggctaaaa gagctttatc tgtggccgat
     4501 taatgccgat caacaaggaa caccgcagca ggtacgtaag gttgttgata tagttaagaa
     4561 aaatcatatc ccggcagtct ttagcgagag tacgatttcc gataaaccag cgcgtcaggt
     4621 tgcgcgtgaa accggcgcac actacggcgg tgtgctctat gtcgattccc tgagcacaga
     4681 aaacggcccg gtacctacgt atatcgacct tcttaaggtt actaccagta cgctggtaca
     4741 gggaattaaa gccgggaaaa gggagaaata atgatgcaat ctgcaggcat tgtcgttaat
     4801 gatgttactg tcacctggcg taacgggcac acagcactgc gcgatgcatc cttcacagta
     4861 ccaagtggat ctattgccgc tctggttggg gtaaacggtt ccggaaaatc gacgttgttt
     4921 aaagcgatta tgggatttgt gcgtctgacc agcggaaaga tatctgttct gggtattccc
     4981 acgcgacagg cgctacagaa aaacctggtt gcctacgtcc ctcagtcaga agaggttgac
     5041 tggtcatttc ctgtactggt ggaagatgtg gtgatgatgg gccgctatgg gtatatgggt
     5101 atgttgcgta tagccaaaaa gagggatcgt cagattgtca ccgatgcgct ggaacgtgtc
     5161 gatatggtgg attttcgcca tcgtcaaatc ggcgagcttt ccggtggaca aaagaaacgc
     5221 gtctttctgg cgagagcgat tgcacagcag ggtgatgtga tcctgctgga tgaacctttt
     5281 accggcgttg atgtgaaaac cgaagcgaaa atcatcagcc tgttgaggga gttacgcgcg
     5341 gaaggcaaaa cgatgcttgt ctcaacgcac aatctcggct cagtcacgac attttgcgat
     5401 tatacggtca tggtcaaagg caccgtcctg gcaagcgggc cgacagacac cacttttaca
     5461 gccgagaacc tggagctggc ttttagcggc gtactgcgcc atgtcacact taacggctcg
     5521 gaagaaagca ttattactga cgatgaacgc ccttttgtgg cacatcgacc gtcagcggtg
     5581 cagagggaag caagatgaac gtgctactgg aacccttcag ctatgaatat atgctcaatg
     5641 cgatgtgggt ctcggcaatg gtcggcggtc tctgcgcttt cctgtcatgc tatctgatgc
     5701 taaaaggctg gtcgctgatt ggcgatgcgc tgtcgcactc tattgtcccg ggtgttgcgg
     5761 gggcatatat gctggggctt ccgttttctc tgggagcctt cttttccggc ggactggcag
     5821 cgggcagcat gctgtttctt aaccaacgca cccgcttaaa agaagatgcc atcatcggcc
     5881 tgatcttctc gtcctttttt ggcctggggc tgtttatggt gtcattgaac ccgacatccg
     5941 tgaacattca gaccatcgtc ctgggcaata ttctggcaat cgatccggcg gatatcctgc
     6001 aactcactat cattggcatt ctctcgatca tcgtgctttt tttcaaatgg aaagatctta
     6061 tggtgacctt tttcgacgag aatcacgccc gtgctattgg attacatccg ggcagattaa
     6121 aaatcctctt cttcaccctg ctgtcggttt cgaccgttgc ggcgctgcaa acggttggtg
     6181 cctttctggt gatctgtctg gtggtgactc ccggtgcaac agcatggttg ttaaccgatc
     6241 gttttccccg cctgctcata attgctgtaa ctattggcag tgtaaccagt ttcctcggcg
     6301 cgtgggtcag ttactttctg gacggcgcga caggtggaat catcgtggtg gcgcagacac
     6361 tgctgtttct tttagcattt gtatttgcgc ccacccatgg acttttggca aatcgtcggc
     6421 gtgcgcataa agcgctggag gatcgcttat gatggcatta ttactggagc cactgcagtt
     6481 cacctttatg agtcacgcac tgctgatttc actggtagtt tccataccct gtgcgctgct
     6541 gtcggtcttt ctggtcctga aaggttgggc actgatgggt gacgcgatga gtcatgcggt
     6601 ttttcctggc atcgtgctta cctggattct ggggttacct ctggctacag gggcattcgt
     6661 cgccggagta ttctgcgcgg tggcgacggg atacctgaaa gacaacagcc gaatcaagca
     6721 agatacagtt atggggattg ttttttctgg catgtttgcc gcaggtctga tcttgtatat
     6781 tgcaatcaaa ccagacgtac atcttgacca tattcttttc ggcgacatgc tcgggataac
     6841 catcggcgat ataatccaga cggtgattat tgccgggctg gttacacttg ttattagcgt
     6901 aaaatggcgg gattttttgc tgttcagctt tgattatcaa caggcgcagg taagcggttt
     6961 gcatacgaga tggctgcatt atgggctgct gtgcatggtt tccctgacca ttgtggcgac
     7021 gctgaaagca gtaggtatca ttctctctat ttctctgctc attgcgcctg gcgccattgc
     7081 agtactcctg acacaacgct ttcatattgc gctcctactg gcgactggca tatcggtaat
     7141 agtgtcaatg actggtgtct ggctgtcctt ttttatcgac agcgcaccag ctccgacgat
     7201 tgtcgtctta ttcgcggtcg tgttcatcat gacgtttacc gttaccagca tcaacgcacg
     7261 cacaaaggga aacgcccata cacaggatct gttatcaccc aattagcaca atatccgagg
     7321 acagcatcgt ccgccagcct acagaagtcc ggaaagccat ggaaggtcgt tgggaggagg
     7381 tacgaattct atcatgcaaa aaatacgtaa aatcgataac gcctagaaat cattcaatac
     7441 tcgcactatc gaaagtttac cagctaaccg cggcacgttc ctgcatacga cgtgccgcag
     7501 cttctgttat tccacgataa aattcctaac cgaattcaaa tccttcagac tattcagctc
     7561 ttccttcagc tcccgctgac ggcgataaat
//
LOCUS       DAJTMK010000179         7553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTMK010000179 DAJTMK010000000
VERSION     DAJTMK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.183"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..61)
                     /locus_tag="OO135_003479"
     CDS             complement(<1..61)
                     /locus_tag="OO135_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399192.1"
                     /translation="MTTTFTGTVSSANSGNYYTI"
     gene            complement(68..328)
                     /locus_tag="OO135_003480"
     CDS             complement(68..328)
                     /locus_tag="OO135_003480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5399193.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            complement(1003..1488)
                     /gene="sixA"
                     /locus_tag="OO135_003481"
     CDS             complement(1003..1488)
                     /gene="sixA"
                     /locus_tag="OO135_003481"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5399194.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1691..3835)
                     /gene="fadJ"
                     /locus_tag="OO135_003482"
     CDS             complement(1691..3835)
                     /gene="fadJ"
                     /locus_tag="OO135_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5399195.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCASHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3835..5145)
                     /gene="fadI"
                     /locus_tag="OO135_003483"
     CDS             complement(3835..5145)
                     /gene="fadI"
                     /locus_tag="OO135_003483"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5399196.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMSVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(5324..5608)
                     /locus_tag="OO135_003484"
     CDS             complement(5324..5608)
                     /locus_tag="OO135_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5399197.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5980..7320
                     /gene="fadL"
                     /locus_tag="OO135_003485"
     CDS             5980..7320
                     /gene="fadL"
                     /locus_tag="OO135_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5399198.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPARQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1965 a         1962 c         1743 g         1883 t
ORIGIN      
        1 aaatggtgta ataattgccg ctatttgcag aagaaacggt accagtaaag gtagtggtca
       61 ttgtttccta ggtatcaaat acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg
      121 tggtgccgtt atttttcagt ttttcggcac cgatatcacc catatcaact tccagtttgt
      181 gatcgtccgg aacgatagaa cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac
      241 cgttgttacc cgcagcgatt gttgccgtag aggtgaacac gcccatcacc attgccgcag
      301 caatagctgt tttaacaaac ttactcatta tttgtcctta aactataaat aattaaaaat
      361 ataaacattg caatacattg attcagtcaa tagccaatgt ttatacccag ccattgaata
      421 cggcatgcga tccaaaaaga acaggtcgta gaaaattgta ttcgttggtg tggggccgca
      481 tagtgatgtt tccacacccc tgatgcaatt taattttact aaagagtaaa tgtagattta
      541 attaatatat tgacagaaga aagattatct taaggacatc cttaaaaaat acggaataag
      601 tatttagaaa aaatagaaag tatatcaata atgaatatca acagcttaaa aaacaaacaa
      661 tgaacatata atgcgattgg catttatcta cttttgacaa tataatatat aaacactata
      721 gccagaattt aatcaagttt caattacatc ctgcctgatt attcaactct attatctgta
      781 ttaataaatg acaatgagtg tcaaacagac attattcatt tcaacatgta actttgggca
      841 aaatcaagat acgcgcagaa gaataatttc cgaatgaatg tagaaagtgt tttatttata
      901 gataaggaga gtcttcgata gtagtgaggt tgacagatat ccgctgcctg atgagcaatt
      961 atgcccaatg aatctacctc attgggcata atttggaaca catcagatag cttttgccat
     1021 cttcagatta cacgggctca tctgccagtt gaatgtacca tttccgcttt catcaagcgt
     1081 tacgctggca atggccgagg tggtaaacat aggcggcgtt tcgcccggac ataactcggc
     1141 aaccagatac ccgactaacg gtaagtgaga gatcaccagt accgaagcaa caccttcatt
     1201 ggtcaacgcc tgcaaatagg cactgaccag accaacatcg ccgcagggcg ttaactccgg
     1261 cagaacttct gcactggaag gcaggttcag acaatcccct acttcttcca gtgtttgctc
     1321 ggctcgcagg aacgggctca ccagaacgcg ttcgatttcc actttttgac ctttcagcca
     1381 gttcgccatc aggcgagatt cgtcacaacc attagtggtc agaggacgaa ccgaatcact
     1441 ggcggcatcg agggctgcgt cgccgtgacg catgataaaa acttgcatat tgcaccgctt
     1501 ttgttaacca gattcgccca ttttctttca tctgaaacca gagaaagaaa acggtggccg
     1561 gcattgtgcc ttatccattc accgaatgaa acgctgtttt ttacctcaat ggcgtaagta
     1621 tagtcaatat gcgtttacat tttgaccaat actccgccat tcagcgcgga ttcatatagc
     1681 tttgaccttc ttattgcagg tcagttgcag ttgttttcca aaaactttcc ccacgcgcgc
     1741 ccatctcgac caaacgctcg caaggggtaa aacgggaacc atactgcgtg gccagtcgtt
     1801 gcattattgc aaccacttcg cccgcgccga gagaatcgat atagcggaac ggtccaccga
     1861 gaaatggcgg aaaaccaatg ccaaataccg caccaatatc cccgtcacgc acgctacgga
     1921 taacctgctc atcaacacaa cgtactgctt cattcagcat caacatcaca caccgttcag
     1981 caacctgcgg tgcggagagt cgcccctgcc cttgtgcgcc aatgagcggg taaatggcgg
     2041 gatcgacctg ttttttgctt ttacgccctt tctgaccata aagatagaaa ccccgaccat
     2101 tttttctgcc tttgcgatcg tcgttcaaaa ttgaagaaac aacatttgca ggcgcgctaa
     2161 aacgttctcc ataagcggct tccagtacag gaataatttt agtcccggtg tcgattccta
     2221 cctcatccaa aagttggatt ggacctaccg gaaaaccaaa tttcactagc gcggcatcaa
     2281 tgtgctctac ccgttcacct ttggtcagca tgcggatagc ttcattaatg taaggcgcta
     2341 agatgcgatt gacgtaaaac ccggctttgt cacgcacgac aatgggcgtt ttgccctgtt
     2401 ttttcgccag ttttaccgta gtggcgatgg tttgcgctga tgtacccgca tgaggaataa
     2461 tctccaccag cggcattttt tccaccggac tgaagaaatg caggccgata acttgctcag
     2521 gtcgcgtggc gtgagcggcg atatcaccaa tcggtaaaga tgacgtattc gaagcaaaga
     2581 tggtatgaga ggcgcaattt tgctcaactt ccgccaccat ctgttgtttc aattcgagat
     2641 tttcaaacac cgcttcaata atcagatcgc gatgggcaaa gccgcgatag tccgtcgttc
     2701 cggagattaa tgccagctgt ttgtcacgtt cgctggcttt gagatgacga cggcgaactt
     2761 tgccctccag ctgatcccaa ctgtacttca gcgcatgatt tatgccctgc gggttgatat
     2821 ctttaattct gaccggaagc cccgctttac aagcagtgac ataagcaata ccgccgccca
     2881 tcaagccacc acctaaaatt cccacgctgt ttaatggcgc aggcggtgca tcactaccgg
     2941 gatctttctt cacgtccgta ctggcaaaaa agatactacg cagcgcctgc gattgtggcg
     3001 tcatcgccag ttcgccaaac gcccgagctt cggcgtcata accgctgctg gtgccctgcg
     3061 ctaatcccgt ttcaacaacc tccaggatgc gttctgtcgc cggataattg ccttgagttt
     3121 tgtgttctgt tttcttgccg accattttga acagcagcgc acgacctaac ggccccgcca
     3181 gaatacgctc gcgtacaggt agagggcggg aagatgggcg atccttcttc gccagctcaa
     3241 cagcgacttc cagcaaaatg gagtgcggaa caacgtcatc caccagcccc agctttaatg
     3301 cctgtttcgc ccgaagttgt tttccggtga ggatcatctc taatgctgtg ctgacgccta
     3361 tcagacgcgg taaacgctgg gtgccgcctg aaccgggtaa caatccaagt tgtacttcag
     3421 gcaaaccgag caccgtttta ggatcgtcag tacaaacgcg accgtggcac gccagcgcca
     3481 actccagccc accacccagg caagcgccat gaatagccgc gataaccgga atgggcaaag
     3541 catgaatctc cgccatcaac tgttggccct gccgcgccag agcttccgct tcttgcgccg
     3601 ttttgcagtt gccgatcatg ttgatgtctg ctccggcgat aaaattgtcc ggtttcgcgg
     3661 agataaacac cacgccgcgc aactctttgt tttcacggag ttgcttaata atggcgcgca
     3721 cctgcgaggc aaattccgcc ttcagggtat tcattttctc atccggtacg tcgatggtga
     3781 tgatagcaat gttgtccaga cgaacattaa gagtaaacgc tgatgccatt tccattattc
     3841 cgcctccaga accattgccg cgccaagccc accggcagca caggcggtaa ctaaaccaaa
     3901 tccaccgccg cgacggcgaa gttcatgcaa tgtctgggta atcatccgcg cgccggtcgc
     3961 cgcgaaggga tgcccgtaag caatcgaacc gccaagcacg ttaaatttgc tatcgtccac
     4021 ttcgccagtg gcatgtgcac gccccagtac ttcacgagca aaacgttcac tacccagcaa
     4081 ctgaatattc gccagtgtct gagctgcaaa ggcttcgtgc atatcgatca atgtcagatc
     4141 gctcattgtc aaaccggcac gttccagcgc cagcggtgtt gaccaggccg gaccgaacaa
     4201 catgtcctgc cagacatcaa tcgcagtaaa tgcgtagctg cgcagatacc ccagcggcac
     4261 cagccctaat tctttcgccc gggattcagt catcaggatc accgctgccg cgccatcggt
     4321 cagcggcgta ctgtttgccg ccgttaccgt tccgtgtttg cgatcaaacg ccgggcgcag
     4381 ctttgcgtaa tcggcaagcg aggaattacc gcgaatattg ttgtcttcga caagcggttg
     4441 tttataagga gggataaagg cagtcatcac ctcttctttg agttttccgt ctgaccatgc
     4501 ctgagcggca cgctgatgcg aacggtgcgc taatgcatct tgctgttctc gggtgatgcc
     4561 gtaggttttc gccatttgct ctgcggtgtc acccatccgc aagccggtag aatattctgc
     4621 tactgcaggc ggtacggaca ttaaatcgcg caaacgcagg cgagagaaga gtttcaggcg
     4681 ctggctcatg gttcgagctt tgttgacatc aaccagcacg cgcgccagtt ttttactgac
     4741 gccaattggc aatacggaag aggaatctgc cccaccggca atccccgctc ggatagttcc
     4801 cgccatcagg ctttctgcga cgtttgcaac cgcctggaaa ctggtagcgc aagcgcggct
     4861 gacgctgtaa gcatcggtat gcacattcat tccggtaccg agaacaattt cacgcgcaat
     4921 gttgggggct tcaggcattt gtacgacctg accaaagacc agctgttcaa tcacttcagc
     4981 ggggatctcg ctgcgtgcca gcagttcgcc taccaccatc ttccctaaat caaccgcggg
     5041 aatgccatga aaagccgtcg cctggcgggc aaaaggcgta cgtaaaccgc taacaatggc
     5101 gatacgatcg ccctggcggg taaccagcgg taaaacctga cccataacac tcccctgtaa
     5161 aaaaataaat aaagtggtct gacctgatca tagtcttaac cattttttac atttagccaa
     5221 gcggagaaaa aggaaagtgg gagctatgac acagtgaaga caggagaaag aaaagaaaac
     5281 gcccctgcca gcaactgaca ggggcgaacg tacccgagag gaattaacgc agacccagct
     5341 ggaagatcag catttcagct tcgcaggcaa aagtgaaatc gatatccagg cgcacaccgt
     5401 cagactcttc agtgaaagtc ggggagattt tgcacggttc ggattccacg ctacgggctt
     5461 tttcagtcaa cgccgccagc gtttgttctg cttctgcgcg gtttgcaaac acgcggctgt
     5521 aagacgcggt gcagtcggag ttgtccataa tggtgccaac atccatacag cagcaaaccg
     5581 gggtttcatc agcactacat ttactcatcg ttgatttcct ctgtatgtgc acccaaggtg
     5641 ccagataaac gttgtggata ttttacgctt ccggaaagtg ctgctccagt tgttaattct
     5701 gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga tctaaaacaa aattcacccg
     5761 aatccgcgag tgcgcaacct ccaaattttg ccagctggat cgcgtttctt agatcatatt
     5821 tgaaaaaaga tggaaacata cttgcaacat tccagctggt ccgacctata ctctcgccac
     5881 tggtctgatt tctaagatgt acctcagacc ctacacttcg cgctcctgtt acagcacgta
     5941 acatagtttg tataaaaata aatcattgag gttatggtca tgagccagaa aaccctgttt
     6001 acaaagtctg ctctcgcagt cgcagtggca cttatctcca cccaggcctg gtcggcaggc
     6061 tttcagttaa acgaattttc ttcctctggc ctgggccggg cttattcagg ggaaggcgca
     6121 attgccgatg atgcaggtaa cgttagccgt aaccccgcat tgattaccat gtttgaccgc
     6181 ccgacatttt ctgcgggtgc ggtttatatt gacccggatg tgaatatcag cggaacgtct
     6241 ccgtctggtc gtagcctgaa agccgataac atcgcgccta cggcatgggt tccgaacatg
     6301 cactttgttg caccgattaa cgaccaattt ggttggggcg cttctattac ctctaactat
     6361 ggtctggcaa cagagtttaa tgatacttat gcaggcggct ctgtcggggg tacaactgac
     6421 cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct taaataatgc atggagcttt
     6481 ggccttggtt tcaacgccgt ctacgctcgc gcgaaaattg aacgtttcgc aggcgatctg
     6541 gggcagttgg ttgctggtca gattatgcaa tctcctgcca ggcaaactcc tcaagggcaa
     6601 gcattggcag ctaccgccaa cggtatcgac agtaatacca aaatcgctca tctgaacggt
     6661 aaccagtggg gctttggctg gaacgccgga atcctgtatg aactggataa aaataaccgc
     6721 tatgcactga cctaccgttc tgaagtgaaa attgacttca aaggtaacta cagcagcgat
     6781 cttaatcgtg cgtttaataa ctacggtttg ccaattccta ccgcgacagg tggcgcaacg
     6841 caatcgggtt atctgacgct gaacctgcct gaaatgtggg aagtgtcagg ttataaccgt
     6901 gttgatccac agtgggcgat tcactatagc ctggcttaca ccagctggag tcagttccag
     6961 cagctgaaag cgacctcaac cagtggcaac acgctgttcc agaaacatga aggctttaaa
     7021 gatgcttacc gcatcgcgtt gggtaccact tattactacg atgataactg gaccttccgt
     7081 accggtatcg cctttgatga cagcccagtt ccggctcaga atcgttctat ctccattccg
     7141 gaccaggacc gtttctggct gagtgcaggt acgacttacg catttaataa agatgcttca
     7201 gtcgacgttg gtgtttctta tatgcacggt cagagcgtga aaattaacga aggcccatac
     7261 cagttcgagt ctgaaggtaa agcctggctg ttcggtacta actttaacta cgcgttctga
     7321 taacgcgttc gcctggataa agtcacctgc atagcaggtg actttaactc ccccacttca
     7381 ccgaaagtag tgtcctcatt gcttacctcc tgagttttgc aaacagcctg ttggcagctt
     7441 gccctttcaa atcaataagc ggtatccaca gacaaacctg aagggaaagg catattttca
     7501 ggcgttctgc tcgtccttct caaagagttt acttttctgc atttccagga tac
//
LOCUS       DAJTMK010000180         7445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTMK010000180 DAJTMK010000000
VERSION     DAJTMK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.184"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..265
                     /locus_tag="OO135_003486"
     CDS             <1..265
                     /locus_tag="OO135_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR5399199.1"
                     /translation="RDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSK
                     MRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            628..1665
                     /gene="dusA"
                     /locus_tag="OO135_003487"
     CDS             628..1665
                     /gene="dusA"
                     /locus_tag="OO135_003487"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5399200.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1799..2041
                     /gene="pspG"
                     /locus_tag="OO135_003488"
     CDS             1799..2041
                     /gene="pspG"
                     /locus_tag="OO135_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5399201.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2206..3189)
                     /locus_tag="OO135_003489"
                     /pseudo
     CDS             complement(2206..3189)
                     /locus_tag="OO135_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
     gene            3272..4687
                     /gene="dnaB"
                     /locus_tag="OO135_003490"
     CDS             3272..4687
                     /gene="dnaB"
                     /locus_tag="OO135_003490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5399202.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4740..5819
                     /gene="alr"
                     /locus_tag="OO135_003491"
     CDS             4740..5819
                     /gene="alr"
                     /locus_tag="OO135_003491"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5399203.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6072..7265
                     /gene="tyrB"
                     /locus_tag="OO135_003492"
     CDS             6072..7265
                     /gene="tyrB"
                     /locus_tag="OO135_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5399204.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1777 a         1802 c         1973 g         1893 t
ORIGIN      
        1 acgtgatatg aagaagtacc tgggagagat gaagcgtctg gctgatgggg gagaaatgaa
       61 ttttgatggc tatctggata agatgtcaca tctggtgaat gaagggacgc tcgatcctga
      121 tattttaagc aaaatgcgag atgctgcacc acaattaatt agcttcgcga agtcgtttga
      181 cccaacctca aaggaagaga ttaaaatact tacagacact tctaaattaa tttatgattt
      241 gttcggggtt aaatcggaga aataatatgt gaagttcttc gatagtatgg aaggcattat
      301 ataaaaggac ccaatattta ttgggttctt ttttctctat caatgctatt agcagggaga
      361 tatatcacca gagtttaatg tgtgattttt tatttatcgt cgaacctgga ttgtttatca
      421 ttggccttaa caaagttaac ggctaataag attatttcca tcacttcgtg agagcttcat
      481 gccttgagag gatctcaatt ttcttttgca atgagacagg cgcttcctgt tgttatggta
      541 tagtaccccg ctattgagcc tcctgaatag tgatgctgaa taacataacc ccatgatata
      601 tcgataaaat aatctctaca tttgaaaatg cacggtaatt ctgaaatgca aaaaatcaac
      661 caaaccagcg caatgcctga aaaaactgac gttcactgga gtggtcggtt tagcgttgca
      721 ccaatgctcg actggacgga cagacattgc cgctatttct tgcgtctgct ttcccgcaat
      781 acgttgctgt ataccgaaat ggtgaccaca ggggcgatta ttcacggtaa aggtgattac
      841 ctggcgtaca gtgaagaaga acatccggta gcgttgcaac tcggcggtag cgatccggcg
      901 gcgctggcac agtgtgcgaa gctggcagaa gcgcgtggat atgatgagat caacctgaat
      961 gtcggctgcc cgtctgaccg ggtgcagaac ggcatgtttg gtgcgtgtct gatgggtaat
     1021 gcgcagctgg ttgccgattg cgtgaaagcg atgcgcgatg tggtgtcgat tccggtgacg
     1081 gtgaaaacgc gtattggcat cgatgaccag gacagctatg aatttctctg cgatttcatc
     1141 aacaccgttt ccggcaaagg cgagtgtggg atgttcatca tccatgcacg taaagcctgg
     1201 ctttcggggt taagcccgaa agaaaaccgt gaaatcccgc cgctcgatta tccgcgtgtg
     1261 tatcaactga agcgtgactt tccgcatctg acaatgtcga ttaacggtgg tatcaagtcg
     1321 ctggaagagg ccaaagcaca cctgcaacat atggatggcg tgatggtcgg gcgcgaggcg
     1381 tatcagaatc cgggtattct ggcggcggta gaccgggaga tctttggttc ctcggatacc
     1441 gatgcctatc cggtggcggt agtgcgcgcc atgtatccgt acattgagcg tgaactcagc
     1501 caggggacgt atctcggcca tattacccgg catatgttgg gcttgttcca gggtattcct
     1561 ggcgcgcggc agtggcggcg ttatttaagt gaaaatgccc ataaagcggg tgcagacatt
     1621 aatgtgctgg aacacgcgct caaactggtg gcggataagc gttaactttt caccaaaaag
     1681 tagtcaaatt caccacgccc tgcgcaccgt cgcggggcgt tttgctgtta aatcaataga
     1741 ttatttttgg catgattctt gtaatgccag caagagattt catatttggg agagcatcat
     1801 gctggaacta ctttttgtga ttggcttttt tgtcatgctg atggtcaccg gcgtttcgtt
     1861 gctgggcatt atcgccgcgc tggttgtggc gacggccatt atgttcctcg gcggtatgct
     1921 ggcattgatg attaagttgc tgccgtggtt actactggcg attgcggtgg tgtgggttat
     1981 taaggcgatt aaagcaccaa aagtgcctaa atatcagcgt tatgaccgct ggcgttacta
     2041 agggattgtg cggatgatca caacctaagg ttttatcctt agaacaaaat aggaattgat
     2101 aatcaaatct gtcactattg cgcctctaac agattcatcg tgctgtaccc tacatacagc
     2161 cgactataaa aagaaagggc ttcccaggtg gaagccctat ttcttttatg gaatcagcag
     2221 gctggaacct tgcgtcgccc ggctttccag aatctcatgc gcacgctgcg catccttcag
     2281 cggatatttc tgctgctcgg cgacatcgac cttaatcaca ccgctggcaa tcaaagagaa
     2341 cagttcatta ctggcctcgg ttaattcctc ccgcgtggtg atatagcctt gcagggaagg
     2401 gcgtgtcaca tacaacgagc ctttttgatt gagaatgcct aagttcacac cggtaaccgc
     2461 acctgatgag ttgccaaaac tgaccattaa gccgcggcgt tgcaggcaat ccagcgaccg
     2521 ttcccaggtg tctctgccca cggaatcgta cactacgcgc actttcttac cgccggtgat
     2581 ctctttcaat cgctcgacca gattctcttc acgatagtta ataacctgcc acgcgccggc
     2641 ttttagcgcg ctctgcgctt tttgcgcggt tcctacggtg ccgataagtt tcgcgcccag
     2701 agcttttgcc cactggcagg caatcaagcc aacgccgcca gccgctgcgt ggaacaggaa
     2761 ctgttcatcg ggtttaattt cataggtttt gcgcagcaga taataaaccg ttaagccttt
     2821 caggaaagat gctgcagctt gctcaaaaga aatagccgca ggcagaatcg ccgctttatc
     2881 cgcattaatg ttatgcacag agctgtaagc gcctaacgcc gactgcgcat agactacacg
     2941 atcgcctgcc ttaatatgct ttacaccact gccgacttta ctcacgatgc ctgccgcttc
     3001 ggtacctgat cagctgggta gtgatggcgg cgggtaaagg ccgctgcgga tatacgtgtc
     3061 gataaaattg atgccgatgg ctttattttc gacctggatt tcattctctg ccggatcggc
     3121 aggagtgaac tctacggctt gaagtacttc cgggccaccg tgcttgtgaa attcaattcg
     3181 tgttgccatg tgtcctccag aacgtatcgt cagggtctgc ttcatatggt aaagtttcga
     3241 cccattcttt atctcggtaa ctccattcac tatggcagga aataaaccct tcaacaaaca
     3301 gcaggctgaa ccccgcgaac gcgatccaca agttgccggg ctgaaagtgc ctccgcactc
     3361 gatcgaagcg gagcagtcgg tgttgggcgg tttaatgctg gataacgaac gctgggacga
     3421 tgtagccgag cgtgtggtgg cagacgattt ttacacccgc ccacaccgcc atatctttac
     3481 tgaaatggcg cgtttgcagg aaagcggtag tcctatcgat ctgattaccc ttgcggaatc
     3541 gctggaacgc caggggcaac tcgatagcgt cggtggtttt gcttatctgg cagagctgtc
     3601 aaaaaatacg ccaagtgcgg cgaacatcag tgcttatgct gacatcgtgc gtgaacgtgc
     3661 cgttgtccgt gagatgatct cggttgcgaa tgagattgct gaagccggtt ttgatccgca
     3721 ggggcgtacc agcgaagatc tgctggacct tgctgaatcc cgcgtcttta aaattgccga
     3781 aagtcgtgca aacaaagacg aagggccgaa gaacatcgcc gatgtgctcg acgcaaccgt
     3841 ggcgcgtatt gagcagttgt ttcagcagcc acacgatggc gttaccggag taaacaccgg
     3901 ttatgacgat ctcaacaaaa aaaccgctgg cttgcagccg tcggatttga tcatcgtcgc
     3961 cgcgcgtccg tcgatgggta aaacaacatt tgcgatgaac ctcgtcgaaa acgcggcgat
     4021 gttgcaggat aaaccagtac ttatcttctc gctggagatg ccttcagaac agattatgat
     4081 gcgttctctg gcgtcgctgt cgcgcgttga ccagactaaa atccgtaccg ggcagctcga
     4141 tgatgaagac tgggcgcgca tttccggcac catgggtatt ttgctcgaaa aacgcaatat
     4201 ctatatcgat gactcctccg gcctgacgcc aacggaagtg cgttcccgcg cacgccgtat
     4261 tgcccgtgaa cacggcggca tcgggcttat catgatcgac tacctgcaac tgatgcgcgt
     4321 accggcgctt tccgataacc gtacgctgga aattgcagaa atctctcgct cgctgaaagc
     4381 actggcgaaa gaactgaacg tgccggtggt ggcgctgtcc cagttgaacc gttctctgga
     4441 acaacgtgcc gacaaacgcc cggtcaactc cgacctgcgt gaatctggct ctatcgagca
     4501 ggatgcggac ttgatcatgt ttatctatcg tgatgaggtg tatcacgaaa acagtgattt
     4561 aaaaggcatc gcggaaatta ttatcggtaa acaacgtaac ggcccaatcg ggacggtacg
     4621 cctgaccttt aacggtcaat ggtcgcgctt cgacaactat gcggggccgc agtacgacga
     4681 cgaataataa ttattttatg aattaggtaa ttaaagcaaa cacttatcaa ggaacacaaa
     4741 tgcaagcggc aactgttgtg attaaccgcc gcgctctgcg acacaacctg caacgtcttc
     4801 gtgaactggc ccctgccagt aaaatggttg cggtggtgaa agcgaacgct tatggtcacg
     4861 gtcttcttga gaccgcgcga acgctccccg atgctgacgc ctttggcgta gcccgtctcg
     4921 aagaagctct gcgactgcgt gcggggggaa tcaccaaacc tgtactgtta ctcgaaggct
     4981 tttttgatgc cagagatctg ccgacgattt ctgcgcaaca ttttcatacc gccgtgcata
     5041 acgaagaaca gctggctgcg ctggaagagg ctagcctgga cgagccggtt accgtctgga
     5101 tgaaactcga taccggtatg caccgtctgg gcgtaaggcc ggaacaggct ggggcgtttt
     5161 atcatcgcct gacacagtgc aaaaacgttc gtcagccggt gaatatcgtc agccattttg
     5221 cgcgcgcgga tgaaccaaaa tgtggcgcaa ccgagaaaca actcgctatc tttaatacct
     5281 tttgcgaagg caaacctggt caacgttcca ttgccgcgtc gggtggcatt ctgctgtggc
     5341 cacaatcgca ttttgactgg gtgcgcccgg gcatcattct ttatggcgtc tcgccgctgg
     5401 aagatcgctc caccggtgcc gattttggct gtcagccagt gatgtcacta acctccagcc
     5461 tgattgccgt gcgtgagcat aaagccggag agcctgttgg ttatggtgga acctgggtaa
     5521 gcgaacgtga tacccgtctt ggcgtagtcg cgatgggcta tggcgatggt tatccgcgcg
     5581 ccgctccatc cggtacgcca gtgctggtga acggtcgcga agtaccgatt gtcgggcgcg
     5641 tggcgatgga tatgatctgc gtagacttag gtccacaggc gcaggacaaa gccggggatc
     5701 cggtcatttt atggggcgaa ggtttgcccg tagaacgtat cgctgaaatg acaaaagtga
     5761 gcgcttacga acttattacg cgcctgactt caagggtcgc gatgaaatac gtggattaat
     5821 cgttctgtaa tatttgattg tctgtgccgg atgcggcgtg aatgccttat ccggccaata
     5881 aaatcctaaa aattcaataa gttgatgttc tttcatgctc ttataaaggt cgtgcctctg
     5941 gcggatgttc gtttgtcatg agtctcactc tgttgctaat tgccgttcgc tcctgaacat
     6001 ccactcgatc ttcgccttct tccggtttat tgtgttttaa ccacctgccc gtaaacctgg
     6061 agaaccatcg cgtgtttcaa aaagttgacg cctacgctgg cgacccgatt cttacgctta
     6121 tggagcgttt taaagaagac cctcgcagcg acaaagtgaa tttaagtatc ggtctgtact
     6181 acaacgaaga cggaattatt ccacaactga aagccgtggc ggaggcggaa gcgcgcctga
     6241 atgcgcagcc ccatggcgct tcgctttatt taccgatgga agggcttaac agctatcgcc
     6301 atgccattgc gccgctgctg tttggtgcgg accatccggt actgcaacaa cagcgcgtag
     6361 caaccattca aacccttggc ggctcagggg cattgaaagt gggcgcggat ttcctgaaac
     6421 gctacttccc ggaatcaggc gtctgggtca gcgatcctac ctgggaaaac cacgtagcaa
     6481 tattcgccgg ggctggattc gaagtgagta cttacccctg gtatgacgaa gcgactaaca
     6541 gcgtgcgctt taatgacctg ttggcgacgc tgaaaacatt acctgcccgc agtattgtgt
     6601 tgctgcatcc atgttgccac aacccaacag gtgccgatct cactaatgat cagtgggatg
     6661 cggtgattga aattctcaaa gcccgcgagc ttatcccatt cctcgatatt gcctatcaag
     6721 gatttagtgc cggtatggaa gaggatgcct acgctattcg cgccattgcc agcgctggat
     6781 tacccgctct ggtgagcaat tcgttctcga aaattttctc cctttacggc gagcgcgtcg
     6841 gcggactttc tgttctgtgt gaagatgccg aagccgctgg ccgcgtactg gggcaattga
     6901 aagcaacagt tcgccgcaac tactccagcc cgccgaattt gggtgcgcag gtggtggctg
     6961 cagtgctgaa tgacgaggca ttgaaagcca gctggctggc ggaagtagaa gagatgcgta
     7021 ctcgcattct ggcaatgcgt caggaattgg tgaaggtatt aagcacagag atgccagaac
     7081 gcaatttcga ttatctgctt aatcagcgcg gcatgttcag ttataccggt ttaagtgccg
     7141 ctcaggttga ccgactacgt gaagaatttg gtgtctatct catcgccagc ggtcgcatgt
     7201 gtgtcgccgg gttaaatacg gcaaatgtac aacgtgtggc aaaggcgttt gctgcggtga
     7261 tgtaatgcag gaaagcaggc tggagttacc cagcctgcag tgaaattaaa ctgtcgtcgc
     7321 tttcactatt tctttgtaga tgattttttt gatgccgttg ttctacgtga gagataataa
     7381 aacgttgtca gttcttttat tgttaagttt atcccaatta actggaattc ctttccggta
     7441 atgct
//
LOCUS       DAJTMK010000181         7390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTMK010000181 DAJTMK010000000
VERSION     DAJTMK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.185"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            191..512
                     /locus_tag="OO135_003493"
                     /pseudo
     CDS             191..512
                     /locus_tag="OO135_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(546..752)
                     /locus_tag="OO135_003494"
     CDS             complement(546..752)
                     /locus_tag="OO135_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399205.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1250..1323)
                     /locus_tag="OO135_003495"
     tRNA            complement(1250..1323)
                     /locus_tag="OO135_003495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1289..1291),aa:Gly,seq:ccc)
     gene            complement(1402..2157)
                     /locus_tag="OO135_003496"
     CDS             complement(1402..2157)
                     /locus_tag="OO135_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5399206.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2572..4869
                     /gene="xdhA"
                     /locus_tag="OO135_003497"
     CDS             2572..4869
                     /gene="xdhA"
                     /locus_tag="OO135_003497"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5399207.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIVHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASHQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4880..5758
                     /gene="xdhB"
                     /locus_tag="OO135_003498"
     CDS             4880..5758
                     /gene="xdhB"
                     /locus_tag="OO135_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5399208.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5755..6234
                     /gene="xdhC"
                     /locus_tag="OO135_003499"
     CDS             5755..6234
                     /gene="xdhC"
                     /locus_tag="OO135_003499"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5399209.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6274..>7390)
                     /locus_tag="OO135_003500"
     CDS             complement(6274..>7390)
                     /locus_tag="OO135_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCR5399210.1"
                     /translation="QHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPD
                     EPHIEQLVGECRVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKP
                     FIAINCAAIPEQLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLML
                     QAKLLRAIEAREILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPI
                     TLPPLRERQEDIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLM
                     EYLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQM
                     IREALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
BASE COUNT         1883 a         1860 c         1765 g         1882 t
ORIGIN      
        1 agtattaaga ctatcactta tttaagtgat actggttgtc tggagattca gggggccagt
       61 ctaataattt cgaaagcaac tcatttttac tctgcaaaca atcagaaaag acttaatttt
      121 gctaagttaa tgcattaatc taaaaacgct ttaaatcaat acataaccat tatctcttgg
      181 gattatttac atgcgcaaaa gtttaatgct gttacttgct tcaacattga ttatttctgg
      241 atgtgcaaca aaaaaacaac tcgttccaac tggtggaagc aaatcagacg gcacagttag
      301 gatgagttat tcttacggta tgtttgaaag tcctgttgta gatccacaac aaggcatgaa
      361 tgcagccaaa gcgcgttgtg ccgcttaggg gatattcagg agccgagcct tttggtggat
      421 tcacttctca atgttcacaa ccttcttcat ctggttgtat gcaaacactt gtgactatcg
      481 aatatcaatg cactggcgat attaagaagt aataattagg ggcttccagc cccttctata
      541 accattcatg gaacagggac aaccgaaatc actttaaacc ctgaaacagc cgttactcca
      601 ttagatggag aattaaatct cacagtatct tttcttactg catcatgaat ttcatctttt
      661 gttggttcat gctctgaatg gaataaaacc tcttccattc taatttgatc accgtctcta
      721 tgctcataag taatcttgta gctaacagtc atatcaacct catgcagggg aattgtgaga
      781 ttattataga caatagaaac cataaagccc cgttggtgat acagcaacaa gcgggagcgt
      841 agcgacttca tcagattgtc agaaagatca caccttatca ggataaccgt gtacccgttg
      901 ggagcgtggc tgaaacgctg atagagcttg catatggttt gcaaggacca atatcaccat
      961 aaacctgttt acgcagccac aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt
     1021 cgatgacatt acaatgccag gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga
     1081 ggtgcaactg ccgccaacgt taggttatac tgtcatctca ccgtgtaact taacgtgaca
     1141 gttactaagg gcggatcaca atggtagaac tcctaagcaa attaaatagg taactgttta
     1201 agttgctgac agagaagact tttttagtct tttccacctg gggagatttt ggagcgggcg
     1261 aagggaatcg aaccctcgta tagagcttgg gaagctctcg ttctaccatt gaactacgcc
     1321 cgcttcgaga tgtgtaaggc attataaacc ttacgctctc cttagcaagt gccgcgctgc
     1381 tgactgctga ttaattcgcc atcagcattt tggcttgctg ccctgcggcg gcaagtaacg
     1441 tagcggatca attgccgttg cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc
     1501 atccgtgctc cccatagtgg cgattttttg cccagtcttc acgctttgcc cattatttac
     1561 cagcatcgtg tcattatggg cgtaagccgt aatgtaatct tcactgtgtt taatcatgat
     1621 gagattaccg tagccacgca gctggttgcc cacatacacc acctttcctg cacccgcggc
     1681 gtaaataggt gtaccccgtg gagctgagat atcaatccct ttattgccgc catctgctgt
     1741 cgaatacggc atgataactt tccctgtcgt tggccataac caacaacgtt gccctactgg
     1801 cggccaggaa gatttcggta ccgctgatga cggtgtaacc gatgcggttt tggtcgttga
     1861 tttggcggtt gatttacgtg tactactgct acttttcgcc ccacccagtt ttagtttctg
     1921 accaacttca atggtgtaag ggggggaaat gccgttcagt cgcgccagtt cttttacgct
     1981 ggttcccgtg gtgcgcgaaa tacgatatag cgtatccccc cgtttcacgg tgtaaacgga
     2041 gccggaatac gttcctgtat cggatgattt gctacccgaa cagcccgcca aaagcagtcc
     2101 aaccgataac aacatcacga tacccagaga ttttttattc aggcgtcccg cactcaaaat
     2161 ctttcctcgc ttaaaaaaca agacgcctta tcatagcagc cagcctcttg atacccaatc
     2221 tcttgcttct ggaaaaacgt ggtaatagag aaagaggtta atttcatttg gcaaagggaa
     2281 gtatcatcag caaaatattc gctgtagagg tatcgaactt cctgactttt taccctattt
     2341 cccatcctga tgcgtaataa tcccaattta tcacattgat tttatcgcca ctcatattga
     2401 tacgtatcac tgcccctatt cagactcctg tttcgtatca tatctgtgtt tttttaacgt
     2461 catcacactt cctacctccc ctaacgctta tcgtcgtttc tggcgtaaat cttgcctgct
     2521 tagactaaat ctttgccata agaaccaaat gttcaaatcg aggggatttc tatggaagcg
     2581 cgggaagcaa ccgctacggg tgaatcatgc atgcgcgtcg atgccattgc taaggtcacc
     2641 gggcgggcac gatatactga cgattatgtt atggcgggca tgtgttatgc gaaatatgta
     2701 cgtagcccta tcgtacatgg ttatgccgta agtattaatg atgaacaagc cagaagtttg
     2761 ccgggcgtac tggcgatttt tacctgggaa gatgtgcctg atattccatt cgctacagct
     2821 gggcatgcct ggacacttga cgaaaacaag cgcgataccg ccgatcgcgc actgctaact
     2881 cgccatgttc gtcatcatgg tgacgccgtt gccatcgtcg tggcccgcga tgaactcacg
     2941 gcagaaaaag cggcgcaatt ggtcagcatt gagtggcaag aattacccgt tatcaccacg
     3001 ccagaagcgg cgctggcaga agacgctgca ccaatccata acggtggcaa tttactgaaa
     3061 caaagcacga tgtcgacggg taatgtccaa caaacaatcg atgccgccga ctaccaggta
     3121 caggggcact atcagacccc cgttattcaa cattgtcaca tggaaagcgt aacatcgctg
     3181 gcgtggatgg aggatgactc gcgaattacc atcgtttcca gcacccagat cccgcacatt
     3241 gttcgccgcg tggttggtca ggcgctggat attccctggt catgcgtacg agtcatcaaa
     3301 ccatttgtcg gtggcggttt tggtaataaa caggatgtac tggaagagcc aatggcggca
     3361 ttcctgacca gcaagcttgg cggcattccg gtgaaagttt cccttagccg tgaagagtgt
     3421 ttcctcgcaa cccgtacccg ccacgctttt accattgacg ggcaaatggg cgtgaaccgc
     3481 gacggaacat tgaaaggtta tagtctggat gttctgtcta acaccggcgc ttatgcatct
     3541 cacgggcact ctatcgcttc tgcggggggg aataaagtcg cttaccttta tcctcgttgt
     3601 gcctacgctt acagttcaaa gacctgctat accaacctcc cctcggctgg tgcgatgcgt
     3661 ggttatggcg cgccacaagt cgtatttgcc gttgagtcta tgcttgatga cgccgcgaca
     3721 gcgttaggta ttgatcctgt tgaaattcgt ttacgcaacg ccgcccgcga aggagatgct
     3781 aatccgctca cgggcaaacg tatttacagc gcagggttgc cggagtgtct tgaaaaaggc
     3841 cggaaaatct ttgaatggga aaaacgccgt gcagaatgcc agaaccagca aggcaatttg
     3901 cgccgcggcg ttggcgtcgc ctgttttagc tacacctcta acacctggcc tgtcggcgta
     3961 gaaatagcag gcgcgcgcct tctgatgaat caggatggaa ccatcaacgt gcaaagcggc
     4021 gcgacggaaa tcggtcaggg tgccgacacc gtcttctcgc aaatggtggc agaaaccgtg
     4081 ggggttccgg tcagcgacgt tcgcgttatt tcaactcaag ataccgacgt tacgccgttc
     4141 gatcccggca catttgcctc acaccagagc tatgttgccg cgcctgcgct gcgcagtgcg
     4201 gcactattat taaaagagaa aatcatcgct cacgccgcag tcatgctaca tcagtcagcg
     4261 atgaatctga ccctgataaa aggccatatc gtgctggttg aacgaccgga agagccgtta
     4321 atgtcgttaa aagatttggc gatggacgct ttctaccacc ctgaacgcgg cgggcagctc
     4381 tctgctgaaa gctccatcaa aaccaccact aacccaccgg cgtttggctg tacctttgtt
     4441 gatctgacgg tcgatattgc gctgtgcaaa gtcaccatca accgcatcct caacgttcat
     4501 gactcagggc atattcttaa tccactgctg gcagaaggtc aggtacacgg cggaatggga
     4561 atgggcattg gctgggcgct atttgaagag atgatcatcg atgctaaaag cggcgtggtc
     4621 cgtaacccca atctgctgga ttacaaaatg ccgaccatgc cggatctgcc acaactggaa
     4681 agcgcattcg tcgaaatcaa tgagccgcaa tccgcatacg gacataagtc actgggtgag
     4741 ccaccaataa ttcctgttgc cgctgctatt cgtaacgcgg tgaagatggc taccggtgtt
     4801 gcaatcaata cactgccgct gacgccaaaa cggttatatg aagagttcca tctggcagga
     4861 ttgatttgag gataacatca tgtttgattt tgcttcttac catcgcgcag caacccttgc
     4921 cgatgccatc aacctgctgg ctgacaaccc gcaggccaaa ctgctcgccg gtggcactga
     4981 cgtactgatt cagctccacc atcacaatga ccgttatcgc catattgttg atattcataa
     5041 tctggcggag ctgcggggaa ttacgctggc ggaagatggc tcgctacgta tcggctctgc
     5101 aacgacattt acccagctaa tagaagatcc tataactcaa cgtcatctcc cggcgttatg
     5161 tgctgcggcc acgtccattg ctggactgca gatccgtaac gtcgctacct acggtggaaa
     5221 tatttgcaac ggtgccacca gcgcagattc tgccacgcca acgctaattt atgacgcgaa
     5281 actggagatc cactccccgc gcggtgttcg tttcgtcccg attaatggct ttcacaccgg
     5341 gccgggcaaa gtgtctcttg agcatgacga aatcctcgtc gcctttcatt ttccgccaca
     5401 gccgaaagaa cacgcgggca gcgcgcattt taaatatgcc atgcgcgacg caatggatat
     5461 ttcaacgatt ggctgcgccg cacattgccg actggataac ggcaatttca gcgaattacg
     5521 cctggcattt ggtgttgccg cgccaacgcc gattcgctgc caacatgccg aacagactgc
     5581 acaaaatgcg ccattaaacc tgcaaacgct ggaagctatc agcgaatctg tcctgcaaga
     5641 tgtcgccccg cgttcttcat ggcgggccag taaagagttt cgtctgcatc tcatccagac
     5701 gatgaccaaa aaagtgatta gcgaagccgt cgccgcggcg gggggaaaat tgcaatgaat
     5761 cacagcgaaa caattaccat cgaatgcacc attaacggga tgccttttca gcttcacgcc
     5821 gcaccaggca cgccgctctc ggaattactc cgcgaacaag gactgctaag tgtcaaacaa
     5881 gggtgctgcg tgggtgaatg tggtgcctgt acggtgttgg tcgacggcac agcaatagac
     5941 agttgcttat accttgccgc ctgggctgaa ggaaaagaga tccgcacgct ggaaggtgaa
     6001 gcgaaaggcg gaaaactttc tcatgttcag caggcttatg cgaaatccgg cgcagtgcag
     6061 tgcgggtttt gtacgcctgg cctgattatg gctaccacgg caatgctggc gaaaccacgc
     6121 gagaagccat taaccattac ggaaattcgt cgcggactgg cgggaaatct ttgtcgctgc
     6181 acggggtatc agatgattgt aaatacagtt ctggattgcg agaaaacgaa gtaaaaggat
     6241 atccggcctg aattcaggcc ggattcactg aggttatgtg tttaacaact catatttctt
     6301 aatcttgcga tagagcgtag caatgccgat gcccagttca tcagcaactt gcttcttgct
     6361 gttatgacgt gaaagcgcct cgcggatcat ttgcttttcc atctcctcca gcgccgtgcc
     6421 gcccgcatca tcgagtgaca ggtgcgcctc actgacctct gttacatcac tttgctccgt
     6481 tgtgccatta ttcagcagat ttggcggcaa tagcgtgctg tcgataactt cacctgaagg
     6541 aactacgtta accagatatt ccatcaaatt gcttaactcg cgcaggtttc cgggccaacg
     6601 atgcttacgc aatatttcga cgacatcggg agcaatgcca ggataaaccg atcccagacg
     6661 acgggtatgc agatgtaaaa agtaatgcac caatagttca atatcttcct gacgttcacg
     6721 cagcggtggc agagttatcg ggataacatt aagtcggtag aagagatctt cgcggaattt
     6781 accttcggca atgaactggg ccaaattctg attagttgca gaaatgatgc gaatgtcgac
     6841 ttgtattggg ctactggcac caatcggcag aatttcacgc gcctcaatag cgcgcagtaa
     6901 tttagcctgc aacattaatg gcatatcacc tatttcatcg agaaacagcg tgcccgtatt
     6961 cgccgcctga atcaaccctg ttttaccgtt ggcagaagcg ccagtaaatg cgcctttaac
     7021 ataaccgaac agttcgcttt ccagaagctg ctccggaatc gcggcacagt tgatagcaat
     7081 aaagggttta ttccgtcttc cgctcaactt atggattgct cgggcgacga cttctttacc
     7141 cgtgccgctt tcaccaacca ccataacgct ggatgggctg ggtgcaatac ggctaatgag
     7201 tcgttttaat tgccgcataa cacggcactc gccaaccaat tgttcaatat gtggttcatc
     7261 aggtgcattt gctacagaaa aactggtatg cgattggtga aatgccatta aaaataattg
     7321 tcggccctga atgttatgca attgaccaat gattaattca cttttatcgt cccacgaaac
     7381 aatatgctgc
//
LOCUS       DAJTMK010000182         7224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTMK010000182 DAJTMK010000000
VERSION     DAJTMK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.186"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..282)
                     /locus_tag="OO135_003501"
     CDS             complement(<1..282)
                     /locus_tag="OO135_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5399211.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRRLTVKDDFNYLGFSESRMDTPVMDDPCPFN"
     gene            complement(284..1521)
                     /locus_tag="OO135_003502"
                     /pseudo
     CDS             complement(284..1521)
                     /locus_tag="OO135_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            complement(1518..1874)
                     /locus_tag="OO135_003503"
     CDS             complement(1518..1874)
                     /locus_tag="OO135_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399212.1"
                     /translation="MQSRSLLLDTGTWDILLDDTGNLAITDNPHAVAQDVACACSTFL
                     GECWYDSTAGIPYWPRILGHWPGTQLVNATLQQEALKLPTVSAAVCQVTADQTRTVTG
                     VLRITDTNNDIFTVLL"
     gene            complement(1887..2564)
                     /locus_tag="OO135_003504"
     CDS             complement(1887..2564)
                     /locus_tag="OO135_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5399213.1"
                     /translation="MAQPLSNPTDVNSEINAQDFMLRQFLGRHAFITLGRVVAVEEGF
                     IEARPMVMGVAADGSPVEHEVIYNIPVWRLQGGGNAVIIPPHVGDIGFLAICDRDISA
                     VKATRQAAMPGSKRTHNYADAIWLGGVLNGDPVQFVVFDDNQIRIVSPWKVEISAPEG
                     VINAPKSFTVNSPQIALNGDTAVSKGLDVTGQSRLSGGSNIGGIDFGNHVHGGVESGG
                     STTQGSR"
     gene            complement(2545..3414)
                     /locus_tag="OO135_003505"
     CDS             complement(2545..3414)
                     /locus_tag="OO135_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399214.1"
                     /translation="MSYRQRKITVEFTLSDGRTFGNGQGNMLTITDASCFASIAVYGG
                     VAGTQITLYIWGMSPAHMTNLSWRGVWRQEQSTANKMRLWADGRLIFEGDITDAYADY
                     NQAPDIPLILTGQIHFNLRNQPAADFSAKGDVAVADIIRALASTVGLGFENQGVSRSL
                     SDPHFSGNVVQQMLDVASAADINIDLGNVEKVTIWPKGQNRNIPPVLISPDHGLTGYP
                     VYTMTGLSATTIFCPDLFTGRPAHLESSLPDMTGDYTITGVIHTITSRTVGGPWSSNC
                     TMMRAEENGTTTQ"
     gene            complement(3411..3713)
                     /locus_tag="OO135_003506"
     CDS             complement(3411..3713)
                     /locus_tag="OO135_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399215.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(3713..4423)
                     /locus_tag="OO135_003507"
     CDS             complement(3713..4423)
                     /locus_tag="OO135_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399216.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGCVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FTVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     ETQPSDADRRSATNRGIT"
     gene            complement(4420..5418)
                     /locus_tag="OO135_003508"
     CDS             complement(4420..5418)
                     /locus_tag="OO135_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5399217.1"
                     /translation="MASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDW
                     LDSHGFFWASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQA
                     TGREAAFGESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPP
                     GLLRSVAITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKL
                     GGLIRFFHGDLAKALAAYNCGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIR
                     SLHPAPVSQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(5745..6332)
                     /locus_tag="OO135_003509"
     CDS             complement(5745..6332)
                     /locus_tag="OO135_003509"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399218.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMR"
     gene            complement(6316..6498)
                     /locus_tag="OO135_003510"
     CDS             complement(6316..6498)
                     /locus_tag="OO135_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5399219.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
     gene            complement(6540..6944)
                     /locus_tag="OO135_003511"
     CDS             complement(6540..6944)
                     /locus_tag="OO135_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399220.1"
                     /translation="MARKEKFITIEGQGRDKGKVFHLTEMSASQAEWWAMRAIMAMGR
                     GGVELPDDVRSLGMAALALEGLKALSKIPPDEARPLMDEMMDCVQFVPDPKKRSIRRP
                     LVEEDIEEIITRLNLRAEVFKLHVDFFATVVS"
BASE COUNT         1711 a         2085 c         1730 g         1698 t
ORIGIN      
        1 attaaacgga cagggatcat ccattaccgg ggtgtccatc ctggactcgc tgaagcccag
       61 gtaattaaaa tcgtccttta ccgtcagccg gcgactgaca cccacaatct ttccccagac
      121 atcaagacca tacttttctg cggtatcgat gttccagata agaccataaa aatcactgat
      181 aaaactgtcg ggggaaagcg ctgcgttaaa gctgttaatg agggcattga gtcgggggct
      241 ggcagcatac tgcgcaagca cagtggcagc cacattctgc acgttatgcc tcctggagtt
      301 tcacactgat atccgacaca tccagaaccg gaatctcatc tatcccgaaa gtgacagccg
      361 ttgtccatga cgaaccgtca cggctcacag taaggcccag aatatcaata tgttccggat
      421 cggttttgta aatgccggca tagtagcgcc cggcagagac aacagaggca acccttgccc
      481 gcagaccacc atctgtacca ttaaatgcgt ccagcacgga ttgctgtacc tgtcgggtaa
      541 tatctgaagg caggtagtca ctttttttca gcgtcacact gacatgcaga ctgacgggtt
      601 tgagtgtctg ccagctgatt acgtattcag gatacggcgg atcgtactcc ttatctgcaa
      661 cagtgaacgt tgtgttgcca ttcatatcaa tacccggcgg agccttacgc cagatggccg
      721 ccgcgatatc tgccgggctg ccgccgtaca cgccaacata aagcgaaccg ggtgttaatg
      781 gatactgact gactccggct ttttgttctg tttttttcgg attatggggg acgtaaacat
      841 ccaccacgtt ttctaccgtg gagagtattt caccccggat ggcttccaga atattccggg
      901 cattacgggc aactgaatta cgtcggcggt tttcaaagtc cgcacgggtt tcctcatcgc
      961 tgcccggcac accggcacgg gcgttagtga cacctgacca gccaggtatt gccttataaa
     1021 ttttattcag cgtccccaca ggacagccga cggggccagt ggacaaattc agaaataccg
     1081 catcaacctg ccctgatgcg ccgatagtga cgtctgactg acatacttat aaccggcctc
     1141 atcctgcccc atactgcccg ccgggatcag cgtgccaacc agcccggtac aggtcgctgt
     1201 tactgtcgta cctgtggccc cacggcgctc caggaaataa atcctgccta ttgcatcctg
     1261 aaagcgacca ctggaaaagt cagggttgat ctggttaacg atatacagca actgatcgtt
     1321 tttatccgca ataatggcgc tttcgctgga tgcaagttgt ccctgcggac tgctcagact
     1381 ggtactcatg gccccgccca gcgccgccga aaaatcagca agcctgccgc tcagaatatc
     1441 cgcttcatcc ggcacattca gcccgctatc cgaaatctgt acagcgggca ctgcggtaga
     1501 aaaagattta ttttcactca tagcagtacc gtaaaaatgt cattattggt atctgtaata
     1561 cgcagcacac ctgttactgt ccgggtttga tcagctgtga cctggcagac agcggcgctc
     1621 acggtcggca gtttaagtgc ttcctgttgc agagtggcat tcaccagttg tgtgccgggc
     1681 cagtgtccga ggatgcgtgg ccagtaaggt atgccagccg ttgagtcgta ccagcactcc
     1741 cccagaaaag tactgcacgc acacgccaca tcctgagcta ccgcatgggg attatcagta
     1801 atggcaagat ttccggtatc atcaagcagg atatcccatg tcccggtgtc aagaagaagt
     1861 gatcgtgact gcatgttttc tcctgtttac cgggatccct gcgtggttga gccgccggac
     1921 tcaacgccgc cgtgaacgtg gtttccaaaa tcaatacccc cgatgttcga accgccggaa
     1981 agcctggact gcccggtaac gtcaagcccc ttactgacgg cggtatcgcc gttcagcgca
     2041 atttgtggtg agttaacggt gaagcttttc ggcgcattaa tcaccccctc aggcgcggat
     2101 atctccactt tccagggaga tacaatccgt atctggttgt catcaaatac cacaaactgg
     2161 acgggatcac cgttgagcac gccacccagc cagatggcat cagcataatt atgggtacgc
     2221 tttgatcccg gcatcgcagc ctgacgcgtg gcttttaccg cactgatatc ccgatcgcag
     2281 atggcaagaa aaccgatatc ccccacatgt ggcgggataa tcaccgcatt accgcccccc
     2341 tgtagtcgcc atacgggaat gttataaatc acctcatgtt caaccgggga accgtctgct
     2401 gcaacgccca ttaccatcgg tcgggcctca ataaatccct cttccacggc taccacccgt
     2461 cccagagtga taaacgcatg acgtccgaga aactgccgca gcataaagtc ctgtgcattg
     2521 atttcgctgt tcacgtctgt cgggttactg agtggttgtg ccattttctt cagccctcat
     2581 catggtacag ttggaactcc acggaccgcc cacggttcgc gaagtaatgg tgtgtatcac
     2641 ccctgtgatc gtataatcgc ccgtcatatc aggcagtgac gattccagat gcgctggcct
     2701 gccagtaaag agatcggggc agaatatcgt ggtggcgctg agtccggtca tggtgtaaac
     2761 cggatagcca gtcagtccgt gatccggcga aatcagtacc ggcggaatat tccggttctg
     2821 ccctttcggc cagatggtga ctttctccac attccccaga tcaatgttaa tatcggcggc
     2881 tgaagcgaca tccagcattt gttgtacaac attaccggaa aaatgcgggt cagacagact
     2941 gcggctgacc ccctggtttt caaatccaag cccgacagtt gacgccagcg cacggataat
     3001 atctgcaacc gcaacatcgc cctttgcgct gaaatctgcc gccggctgat tacgcagatt
     3061 aaaatgaatc tgtcctgtca gaatgagcgg aatatccggc gcctggttgt agtcggcgta
     3121 tgcatcagta atatcacctt caaaaataag ccgcccgtcg gcccacagcc gcattttatt
     3181 tgcggtactc tgctcctgtc gccacacacc gcgccagctt aaattcgtca tatgcgccgg
     3241 tgacattccc cagatataca gcgtgatttg tgtaccagcc acccctccat atacggcaat
     3301 actggcaaaa cagcttgcgt cggtaatggt cagcatattg ccctggccat taccaaacgt
     3361 tcgtccgtct gaaagagtga actccaccgt tattttacgt tgtctgtagc tcatatcagt
     3421 tcctcaggta acagccagta gagccggtac cgggatccga gcccccgcca gtcagggtca
     3481 ctattcccct ccgtgtcggt aaaaaacaga tccccctgaa acggcaggta tccgtaccgg
     3541 acaatccggt tattattcag acacagcacg ccatacaggc acggttcacc attaacaatg
     3601 atatcgatat acatccccgt agtacgctga ttcaggcgaa tggtgcagac ctgagcgccc
     3661 agtgtcaccg taaactgctg ggctttaacg ggagataaaa caatttccag catcaggtga
     3721 tccccctgtt cgtggcgctt cgtctgtcag cgtctgacgg ttgtgtctcc gacgccgtaa
     3781 ctggctgcgt tttaaccgat gcggcccctt ttgctttatc gttgtccgtg ggagacgact
     3841 tatccgtact tcccactgac acctctcctg tattcattac ggtctgaaat accgcagtaa
     3901 ccgtcagtaa ggtcggacca ttattacttc gcattcggta gtcatatttc acaaggtcgc
     3961 aggatgtcca tgtcttgtcc ggcgtctcaa tatcgtagag tcctgccgtg gtacgcatcc
     4021 tttcaagcgt ttccagcaca ttcgtccgcg aggtggtgga aaaatttgtc aggttcggga
     4081 cggatccgga aaaggccgtc cacccctcta cagtgaaagt cacatgcagt tccggcgatc
     4141 tctggatttt attaaaggtg gtataagccc cctgctccac ggggacggtg gaaacagaag
     4201 cctccgcgcc cacctcaacg acaacaaaag aatccgggga gaaaggtttg ccacccttct
     4261 gatgggcacc tgccggatca ttccgtgcgt aataaatacc gaatgacggt gccagtacac
     4321 tattaatgag tcccaggaca cagccgccac gaactgcact cagtacgtta ctttcactga
     4381 gcgaaaagtt attcagggaa atattatcga aagaaaaact catcctgtta ccccgctgga
     4441 ataaaccgac acaagcgccg aattcgtgat acgccgacgt gcgtcatcgg taataccctt
     4501 cacattgtcc gacgttgtgg tgacattcag tgtcccgata tgcgtggttt ccgttacggt
     4561 ggactgcgat acaggcgcag gatggagcga ccgtatggcc attgccgcac ccggataagg
     4621 caggttcgcc agcacccgcg gaatatagtt gcgggtctcc tccggagcag cagccagtcc
     4681 cttacgctga acatttccct ctccacagtt atatgccgcc agagccttag ccagatcgcc
     4741 atgaaaaaaa cgtatcaggc caccaagttt tctcgcggcg gcatcagcgg ctttttcggg
     4801 atcaaaggca tcgttccccc tcagaccaaa ttcactggcc gtcttcggca tgaactgaaa
     4861 cagtcccatc gcaccagcag gtgagacggc aaactgatta ccacgggatt cggtgatcgc
     4921 aacgctgcgc agcagtccgg gcggcaggtt atatttttcc tccagttgag acagtttcgg
     4981 ttgcagccag cctaacaggg cttccccggt cttcgtcggt cgctggcggt tttgcatggc
     5041 attaccgagt ttttcctgcg tcgcacgcat accctgtagc caggattcac cgaaggctgc
     5101 ttccctcccg gttgcctgag aagcctgagt atccagcatg ccctgctgcc atactgtggg
     5161 tgattgtgcc cgggtgatgt tgccaggctt ttctccggca tccagttttg tctgatacgt
     5221 ctccatctct ttcttattga aaaagaaagt cccgtctgaa gcccagaaaa aaccatgtga
     5281 atccagccag tccttatttt ccctgccaac aatggatgtc agtaatccgt caacaaccgg
     5341 gtaaagcgcc gttatcgcaa aaagaaggcc tccggggcca ctgagggctt ttgtcagccc
     5401 ccgcacccat gacgccactt tcagcccgat cagcgtaata atgacattct gccagccccc
     5461 cagttctccg gcagccttat tcgctaggga ggccactgac tcaacgtcat tcagaaatgt
     5521 ggtgataaac ctgttcactt cctccggatg ttgctgcatc cagtcaccga gtgtctccag
     5581 ccagtggctg agcttcagcg cgtacggcat caacgcggtg ctaatcgtca tcccgatgcc
     5641 ggaccaggtc tgatcgagct caacaagctc ttttcgtagc ttacgggcca gcgctgtacg
     5701 ccccggcgta acctgcgaac gggaagtaaa ttcgtccaca tctttcagcg catgatggct
     5761 tcagtgctgt cagtatttaa atcaacacta atgcccgccc tggctgcaac ctgaaacaaa
     5821 tcctgtaaca caggatcaaa ggaccttccg gctctgaagg ccgcttttgc atttgcaagc
     5881 cgggaaaatg ccccagacat ttccccttca tcaacattat ttgcccgcgc agcccgtaac
     5941 catccgtcca gatttctggc cttcatgcca aaagcatcgg attccacgga cagacggttc
     6001 agatcacggg caaaacctgt taccaggttc ttcagccccc ccagcgtcag actgacaccc
     6061 gcaagagcca gaatctcgct acggattcct gaaaaaaaac gggacgcccg tttcccggca
     6121 tcttccattc gacgggccgt tttatcagca tcttttccgg ttttttccag tgattcactg
     6181 gttttcttct gtccctgact gaatgtgtca gcgacacctt ccatcaccgt cgtgagccgg
     6241 ttaagggcag catgtactgc ctgttccccg gcagtaaagt ccctgtcatc aatcccgagg
     6301 gacagaacaa gctcatcaag tacctgcgcc atttctgttc tcctgtatca cctgttcgtt
     6361 atgggcatcc acatgaatta tttccagcag atcccataag tcctgcacac caaggacaga
     6421 atccagttca gcttttgaag ccttaccgga ggagataacg gtcgcaatgg tacggggaac
     6481 attaacgtaa tccaccaccc cgaacggtct gtcggggtcg agataacgcg gggggatatc
     6541 tagctgacga cggtagcgaa aaaatccaca tgcagcttaa agacctccgc acgcagatta
     6601 agacgggtaa tgatttcttc aatatcctct tcaacaagcg gtctccggat gctgcgtttt
     6661 ttcggatcgg gaacaaactg cacacagtcc atcatttcat ccatcaacgg acgcgcctca
     6721 tcaggcggaa ttttcgataa tgctttcagc ccttcaaggg caagcgcagc catccccaga
     6781 ctacgaacat catccggtaa ctccacgcca ccacgcccca ttgccataat ggcacgcatc
     6841 gcccaccatt ccgcctgtga ggcagacatt tcggtaagat gaaatacctt gcccttatcc
     6901 cgtccctgac cttcaatagt gataaatttc tctttacggg ccatcagtta aaaacctccg
     6961 gagtaatggt ttcccactca ataaccgcct gccctggctg caacgtacga gctgcatcag
     7021 gcagcgcttt ccattgtttg agtacgccat tcacgcaggt atatttacgg ccaatcgccg
     7081 gaagcaggac gacagcgtta caacggaata cagcccgact ggcccggatg tggttgacca
     7141 ggtatcaaaa atatcccggc tgggggaatc cggcatgata tgaaacgtct ggataatgtt
     7201 gctgtacaca aatcccgcgg acag
//
LOCUS       DAJTMK010000183         7190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTMK010000183 DAJTMK010000000
VERSION     DAJTMK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.187"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            392..1366
                     /gene="hemB"
                     /locus_tag="OO135_003512"
     CDS             392..1366
                     /gene="hemB"
                     /locus_tag="OO135_003512"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5399221.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1472..2323)
                     /gene="tauD"
                     /locus_tag="OO135_003513"
     CDS             complement(1472..2323)
                     /gene="tauD"
                     /locus_tag="OO135_003513"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5399222.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(2320..3147)
                     /gene="tauC"
                     /locus_tag="OO135_003514"
     CDS             complement(2320..3147)
                     /gene="tauC"
                     /locus_tag="OO135_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5399223.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3144..3911)
                     /gene="tauB"
                     /locus_tag="OO135_003515"
     CDS             complement(3144..3911)
                     /gene="tauB"
                     /locus_tag="OO135_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5399224.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHDSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3924..4886)
                     /gene="tauA"
                     /locus_tag="OO135_003516"
     CDS             complement(3924..4886)
                     /gene="tauA"
                     /locus_tag="OO135_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5399225.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5502..6173
                     /locus_tag="OO135_003517"
     CDS             5502..6173
                     /locus_tag="OO135_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR5399226.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     IPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            6183..7190
                     /locus_tag="OO135_003518"
                     /pseudo
     CDS             6183..7190
                     /locus_tag="OO135_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         1798 a         1899 c         1719 g         1774 t
ORIGIN      
        1 tgtggacata tataatttat ggagaaaaac acgcatcaaa ttgataaaag gacgatattt
       61 caacatgttg cagataatat tatttttgat acatacgcgt tataaatatc aaaaacacaa
      121 accagataaa tatcttatca atatacaaca aagtttgaat tattcaattt atagcagcac
      181 aaccaaacgc caaaaaaaat aataaccaag agctataaat aaagattaaa agtattttca
      241 gtgaatatga acttcttgct atttctatta caaagatgtc ctggtctctc tttcattctc
      301 ttcttgtcaa catcacgaca actttcgtaa aacactcctg ccccgcttca ggtatactgt
      361 gcaccttaat tccacaaaca tcaggcagac catgacagac ttaatccaac gccctcgtcg
      421 cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag acaacactta gccttaacga
      481 cctggtgttg ccgatctttg ttgaagaaga aattgacgac tacaaagccg ttgaagccat
      541 gccaggcgtg atgcgcattc cagagaaaca tctggcacgc gaaattgaac gcatcgccaa
      601 cgccggtatt cgttccgtga tgactttcgg catctctcac cataccgatg aaaccggcag
      661 cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg cgcatctgca agcagactgt
      721 gccagaaatg atcgtcatgt cagacacttg tttctgtgaa tacacttctc acggtcactg
      781 cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact ctggaaaatt taggcaagca
      841 agccgtggtt gcagctgctg caggcgcaga cttcatcgcc ccttccgccg cgatggacgg
      901 ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc tttaaagata cggcgattat
      961 gtcgtattcg accaagttcg cctcttcctt ttatggcccg ttccgtgaag ctgccggaag
     1021 cgcattaaaa ggcgaccgca aaagctatca gatgaaccca atgaaccgtc gcgaggcgat
     1081 tcgtgagtca ctgctggatg aagcccaggg cgcagactgc ctgatggtta aacctgctgg
     1141 agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact gaattgccga ttggcgcgta
     1201 tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg ctggcgggtg ctatagatga
     1261 agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt gcgggtgcgg atctgatttt
     1321 cagctacttt gcgctggatt tggctgagaa gaagattctg cgttaatttt tttcctctca
     1381 gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc cctcacccta accctctccc
     1441 caagggggcg agggaaccgt ccgcactcgt atcaccccgc ccgataaaac ggtttatccc
     1501 caagaatcgt cacccgatgc ataatccgcc gctgtggcag gtaatcggca ttggcatagt
     1561 gctgagtcac gcggttatcc caaatcgcaa tatcattcgg ttgccagcgc caacgcacct
     1621 gaaactccgg tttagtgata tgggcgaata aaaagcctaa caaggcttcg ctctctttct
     1681 cgctgacatc gacaattcgc gtagtaaagc cttcattcac aaacagcgcc tgtttaccgc
     1741 tcaccggatg cgttcgcacc accggatgta gcaacggagg atttttcgcg accgcctcgc
     1801 gccagcgttg atgttcctcc tcggttttgc ggtatttgta ttccgggaac gatttacgga
     1861 aatcatgctc cgcacgcagc ccgctcagca gctggcggaa gggaacagag agcgcctcat
     1921 aggccgcaat accgctggtc cagagcgtat caccgccggt cgaaggtaat tctttagctg
     1981 ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac atcggtatgc cagttgtcgt
     2041 tatctggcgg attatcgtta tgggtatcca gcacgatgat ctcgtcaacc ccttcggcat
     2101 gagggtaaac agggtgaata tgcagttcgc caaaacgctg ggccagcgcg cgttgctgct
     2161 gcggcgtaat ggcttgatcg cgcagaaaca ccacctgatg gcgcagcacc gcatggtaaa
     2221 gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc ggcacccgaa atttgtgcgc
     2281 cgatatacgg ccccagcggg gtaatgctca gacgttcact cattgtactt ctccatgcca
     2341 gggcgtcaga cggcgctgta acgcgcgcaa acccagttct aaaagaaagg cgataatcgc
     2401 aatcaccgcg attcccgcca gcaccacgtc agtcgcgaga aattcacccg ctgactgaac
     2461 cataaatcct aaaccgcgcg tcgcggcaat cagctccgcc gctaccagcg tagaccagcc
     2521 cacacccaga ccaatgcgta atccggtgag gatttccggc agcgcgccgg gcaaaatgac
     2581 aaaccacagc acctgcgcac ggctggcacc cagcgactgg gcggcacgaa tgcgaacctg
     2641 ctgcgcgctt ttcacccccg ccagcgccga catcgccacc ggtgcaaaaa tcgctaaata
     2701 gatcagtaag atcttcgagg tttcaccaat accaaaccag atcaccatca gcggcaaata
     2761 agccagcggc gacaccggac gataaagttc gattaccgga tccagaatgc cgcgcaccgt
     2821 cgggcttagc cccatggcga tcccgaccgg aataccgatc accaccgccg cgagtagcgc
     2881 cagcacaatg cgcgtcaggc tggctgccag atgctgccac agcgtggcgt ccataaatcc
     2941 ctgcggtccg gcaatggtga gcagttttgc cagtacttgt tgcggcggcg gcaaaaacag
     3001 cgggctaatc agttgcagcg ccgccaccgt ccaccataca gtgagtaaaa ccgctaacgt
     3061 gccaatgctt aaggtcacct gacgcgagag cggccagcgc catttcagcc gccgcgaatg
     3121 cagtttttca ttaatgagca cactcatgag aacgcctccc gttgctcaaa tacgcggctt
     3181 aaaacatatt cgcgcatggc gatgaattgt ggatcggact tgatgctgcg gctcgactct
     3241 cccgcaacaa agcggcgagc aaagttgagc ggcagccgct ccagcacacg gccagggccg
     3301 gatgaaagca gaaccagttc agtcgccata aacaccgctt cttctatatc gtgggtaatc
     3361 aacagcacct gcttgcccgt ctcctgccag agtttcagca gcagagtttg catctggtcg
     3421 cgggtgaagg cgtccagcgc accaaacggt tcgtcgagta ataacagctg gggattcgcc
     3481 gccagcgcgc gggcaatccc cacccgctga cgttgaccac cggaaagctg ccagatatag
     3541 cgtttttctg cgccttccag ccccactttt ttcagcacct ggtgcgcgat ttccagtcgc
     3601 tgcattttct ctatacctgc caattgcagg ccgaacgcca cgttgtcctg tacattgcgc
     3661 cacggtagca gcccttcatt ctgaaaaacc accccgcgct ctgctcccgg tccctcaata
     3721 cgcttacccg ccagttgaat gctgtcatgc tgataaggca caaaaccggc aatcagattc
     3781 agcaacgtgg ttttaccgca gccggacggc cccagcacca ccagtagctc gccgctttcc
     3841 agcgtcaggt tgatatcttc cagtgccggt ttgccgccat aatcggcata gagatgagag
     3901 atttgcagca tctgcgcctc cttttattgc acgaagcgcg aggtaacgta ctggctgtaa
     3961 tcattcgcta cagccgggac cttgccctgc tctttcaaaa actgcgcggt gtcgatgatt
     4021 actttgttca ccggtccggt cagttctgcc gtttgttgct gcggcgtcag ataggtattc
     4081 cccttaacca gccccggaac gtcaccttcc ggcacgccgc ttaaacgcgc cagtttgctg
     4141 atgttttccg gctgtttcag ccacgcgtct gggttagcaa tgtacggttg ctgagcatcg
     4201 atggcgcttt tagcgaacgc tttcacgacc tcaggatgtt tctcggcaaa atctttgcgc
     4261 accacccaga cgtccagcgt tggcgccccc cactgcccga cctgttcaga atcggtcaac
     4321 accttgccgt ctttttccag ggcgttaacc gccggtgccc agacataagc accatcaata
     4381 tcaccccgct gccatgcggc gataatcgcg ggcggctgca ggttcacaat ctccacttgc
     4441 cctggtttaa tcccccagtg tttcagcgcc gccagcaggc tgtagtgggt ggtggagata
     4501 aacggtacgg caatgcgttt gccaatcaaa tcttcgggtt tgctgatagt tttctttacc
     4561 accagcgctt cggagttacc cagttttgac gccagcaaga agacttcaat cggcacctgt
     4621 tggctggctg caaccgctaa tgggctggaa ccgaggttgc cgatttgcac gtcgccagaa
     4681 gccagcgccc gcacgatgct ggctccgctg tcaaacttac gccagtccac gctcgccccg
     4741 ctctctttag caaaggtgtt gccggcctga gccactttcg ccggttcggc tgaggtttga
     4801 tacgccacgg tgacgttcac cgcctgtgcc tgaaaagcga tgaatgccag tgcggcaaga
     4861 agtgtgttac gcgatgaaat tgccatgatt gtctgctccc cagtcttgtt atgggagcag
     4921 tattcaggaa taaaaacatt cattaaaaga attagtcgtt atcgcacaga tgattttatt
     4981 cttagcaaaa aaacggcaaa ggaaagatta aatatgcaat tactccgttc taacaattgg
     5041 gaaaattact acacttcatt ttagtgatgc ataaacaacc acattacgag atttattact
     5101 tcattgtttc tatcaatatc aatacatata ataaaactat tatattcgca ttttatatat
     5161 cactgtatca ttctcaatca ctcgcatatt acattcacat aatgtatata aacaatactt
     5221 gttcacagaa catatttttt aaatgcaatt aattgacaaa ctaaatcaca tctaaataca
     5281 atgtataaaa caaaataact atacatacca aacatacaaa cacttaatat atgaattgca
     5341 taaaaacatt acccagtcga acctccgggt ctgaaagccc ttgcttttca gttattagca
     5401 acatttaaca tttataaatt aacatctggc tgcaccagag attacgtctc tgagtgcatc
     5461 gttgtgcttg tagtataata aataaaatgg agtgtcatta agtggataag gttttagatt
     5521 cagccctcct ttcttcggca aataaaagaa agggtatcct tgctattggc gcacatcctg
     5581 acgatataga attaggctgt ggcgcatcgc ttgctcgtct tgcgcaaaaa ggaatttata
     5641 tcgcagcagt ggtgatgact accggcaact ctggtacaga tggaataatc gatcgccatg
     5701 aagaatcgcg caacgcccta aagatattag ggtgccacca aactattcat cttaattttg
     5761 ctgacacccg cgctcattta cagctcaatg atatgatttc cgccctggaa gacatcatta
     5821 aaaatcaaat tccttctgat gttgaaatca tgcgggtata taccatgcat gatgccgacc
     5881 gccatcagga tcatctcgct gtttatcaag cttcaatggt tgcctgccgc actattccac
     5941 aaattctcgg ctacgaaacc ccaagtacct ggctttcatt tattcctcag gtttttgaat
     6001 ccgttaaaga agaatatttc acagtcaaac ttgctgcatt aaaaaaacat aaaagccagg
     6061 aacgacgtga ttatatgcgt catgatcgcc tgcgtgcagt tgcacaattt cgcgggcaac
     6121 aggtcaatag cgatctgggt gaaggctttg taattcataa aatgattctt tgagcaaatt
     6181 gcatgaaaac ctggatattt atctgtatgt ccatagcaat gttgctatgg tttttaagta
     6241 cgctaagacg taaacccagt caaaagaaag gctgtattga cgccattata cctgcgtata
     6301 acgaaggccc gtgtctggcg cagtcactgg ataatctact gcgtaaccct tatttttgcc
     6361 gggtaatttg cgttaacgac ggctccacgg acaataccga agcggtcatg gcggaagtca
     6421 aacgcaaatg gggcgaccgc tttgttgccg tcacgcaaaa aaaataccgg taaaggtggt
     6481 gcgctgatga atggcctcaa ttatgccacc tgcgaccagg tttttttaag tgatgccgac
     6541 acctatgttc cgcccgatca agacggaatg ggctatatgc tggcagaaat tgagcgcggg
     6601 gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct
     6661 acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgtac
     6721 tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac
     6781 ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc
     6841 acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggcta
     6901 cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta tcttcagtat
     6961 atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta cctggtttaa
     7021 tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctgggt
     7081 cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt
     7141 gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
//
LOCUS       DAJTMK010000184         7184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTMK010000184 DAJTMK010000000
VERSION     DAJTMK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.188"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1242
                     /locus_tag="OO135_003519"
     CDS             <1..1242
                     /locus_tag="OO135_003519"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HCR5399227.1"
                     /translation="TAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAG
                     YGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDL
                     VLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQA
                     ELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVL
                     SGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGT
                     SYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNS
                     YLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRD
                     IEMRADGEVAVDAGVDTQWGH"
     gene            1242..2039
                     /locus_tag="OO135_003520"
                     /pseudo
     CDS             1242..2039
                     /locus_tag="OO135_003520"
                     /inference="COORDINATES: protein motif:HMM:NF015188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2199..3032
                     /locus_tag="OO135_003521"
                     /pseudo
     CDS             2199..3032
                     /locus_tag="OO135_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3160..3882)
                     /gene="ubiG"
                     /locus_tag="OO135_003522"
     CDS             complement(3160..3882)
                     /gene="ubiG"
                     /locus_tag="OO135_003522"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HCR5399228.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHMQNK"
     gene            4029..6656
                     /gene="gyrA"
                     /locus_tag="OO135_003523"
     CDS             4029..6656
                     /gene="gyrA"
                     /locus_tag="OO135_003523"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5399229.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLGEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSVA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6805..>7184
                     /locus_tag="OO135_003524"
     CDS             6805..>7184
                     /locus_tag="OO135_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCR5399230.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1725 a         1770 c         2052 g         1637 t
ORIGIN      
        1 actgccgaag gttatggcgg ggcgatatat accaatagcg cgacggctcc ctatcttatt
       61 gatatttctg ttgatgacag ctacagccag aacggcggcg tgttagtgga tgagaacaac
      121 agcgcagcag gctatggaga tggtccttcc actgcggcgg gtggctttat gtatctcggc
      181 ttaagtgaag ttacctttga tattgccgac ggaaaaacgc tggttatcgg caatacagag
      241 aatgacggag ctgttgactc tattgctggt accgggttga tcaccaaaac aggttccggc
      301 gatctggtac ttaatgcaga taacaatgac tttactggcg agatgcagat tgaaaacggt
      361 gaagttaccc tgggccgcag caactccctg atgaatgtcg gcgatacgca ttgccaggac
      421 gatccgcaag actgctacgg tctgacgata gggagtattg ataagtacca gaatcaggca
      481 gagctaaacg ttggctccac ccaacaaacc tttgtgcact cattgacggg ctttcagaat
      541 ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggtag ttttgctggc
      601 accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctacgt gctgtcgggg
      661 gcgcagtcga tggcgctaac cggcgatata gtcgttgatg atggtgcggt gctttcgctg
      721 gaaggcgacg cggcagatct cgccgctctc caggacgatc cgcagtcgat cgtgttaaac
      781 ggcggtgtgc tcgatctctc tgatttctcc acctggcaga gcggcacatc atacaatgat
      841 ggccttgaag tcagtggcag cagcggaacg gttatcggca gtcaggatgt ggtagatctt
      901 gcaggtggcg acaatttgca tatcggcggc gacgggaaag atggcgtcta cgtggtggtc
      961 gatgcgagcg acgggcaggt aagtctggca aacaataata gttatttggg cacaacacaa
     1021 atcgcctccg gtacgctgat ggtgagcgac aactcgcagc ttggagatac ccactataac
     1081 cgccaggtta tctttaccga taagcaacaa gaaagcgtga tggagattac tgccaatgtc
     1141 gatactcgct ctacgacgac tgagcatggg cgtgatattg aaatgcgcgc cgacggtgaa
     1201 gtggcagttg atgcgggggt agacacgcag tggggccact gatggctgac agcagcgggc
     1261 agcatcagga tgagggtagc acatttacta aaacgggggc gggtacgctg gagctgaccg
     1321 ccagcggtac aacgcagtcg gcagtacgtg tcgaagaagg caccctgaaa ggcgatgttg
     1381 cggatatctt tccttatgct tcgtcactgt gggtcggtga tggggcgacg ttcgttactg
     1441 gcgcggatca ggatattcag tctatcgatg ctacttccag cggcactatc gatatcagcg
     1501 atggtacggt tttgcgtctg accgggcagg atacttccgt cgcccttaat gcctcattgt
     1561 ttaacggcga tgggacgctg gtgaatgcta ccgatggcgt gacgttgaca ggtgagctta
     1621 ataccaacct tgaaactgac agcctgacct atcttgccga cgtgacggtt aatggtgatc
     1681 tgaccaatac gtccggtgcg gttagcctgc aaaatggcgt cgctggcgat acgctaacgg
     1741 taaacggtga ttataccggc ggcggtacgc tactgttcga cagcgaatta aacggcgatg
     1801 actcggtaag cgatcaattg gtgatgaacg gtaatactgc tggcaacaca actgtggtgg
     1861 ttaactccat tacagggatt ggtgagccga catcgacagg cattaaagtg gttgatttcg
     1921 cagctgatcc cacgcagttt caaaacaatg cacagttcag tctggctggc agtggctacg
     1981 tcaacatggg agcatatgac tacacgctag tggaagataa caacgactgg tatctgcgat
     2041 cgcaagaagt aacgccgcca tcgccatctg atccagaccc ggacccaaca cccgatcctg
     2101 acccaacacc cgatccaatc cccgcttacc agccggtgct gaatgccaaa gttggcggtt
     2161 atctcaataa cctgcgggcg gcaaatcagg cgtttatgat ggagcgacgc gatcacgctg
     2221 gtggcgatgg tcagacgctg aatttacgtg ttatcggcgg acgttatcat tacacggcgg
     2281 cagggcaact ggctcagcag gaagacactt ctacggtgca gcttagcggc ggcttgttta
     2341 gcgggcgctg gggcacggat ggcgagtgga tgcttggggc ggttggtggc tacagtgata
     2401 atcagggcga cagccgctcg aatatgaccg gaactcgcgc cgataaccag aaccacggtt
     2461 atgccgttgg gctgacctca agctggtatc agcacggtaa ttagaagcag ggggcctggc
     2521 tggatagctg gctgcaatac gcgtggttta acaatgatgt ttccgaacat gaagatggcg
     2581 tggatcgtta ccattcgtcg gggattatcg cctcgctgga agccgggtat cagtggttac
     2641 cggggcgtgg tgtggtgatt gaaccgcagg cgcaggtgat ttatcaggac gtgcagcagg
     2701 atgattttac cgccgctaac catgcgcgcg tgtcacaatc gcagggggat gatattcaga
     2761 cgcggctggg tttacacagc gaatggcgta ccgcggttgg tgtcacacca acattagatc
     2821 tgaattatta tcacgatccc catgcgacgg aaattgaaga agatggcagc actatcagtg
     2881 acgatgcggt gaagcgacgg ggtgaaataa aagtgggagt cacgggcaat accagtcagc
     2941 gagtgtccct gcgcggcagc gtggcgtggc agaaagggag tgatgatttt gcccagacgg
     3001 cagggttttt gtcgatgacg gtgaaatggt aatcactgtc tagtcggcac ggttgccgga
     3061 tgcggcgtaa acgccttatc cggcctacgg ggagcatttg taggcctgat aagacgcggc
     3121 aagcgtcgca tcaggcattg gctcggccaa acaacaacct cacttattct gcatgtgcag
     3181 catatagttc acatccacgc cggggccgag tttaaaagta ttagtgagcg ggttgtaatg
     3241 tagcccggtt atatgtcgct ctttaaggct tgtctgatct acccagccaa gcagttctgc
     3301 cggtttaata aacttcttca catcatgcgt gcctttgggc accatgcgca aaatatattc
     3361 cgcgccaacc accgccatca gccatgactt gccgttgcgg ttaagcgtcg agaaaaagac
     3421 atcgccgcct ggtttcacca gttgcgcaca ggctctgacc actgactgcg gatcggggac
     3481 gtgctccagc atctccatgc aggtcaccac atcatactgc ccggcatgtt ttgccgcgtg
     3541 ctcttccacg gtttcctgca cataatccac ctgaatgccg ctttccagcg cgtgcagttt
     3601 tgccacctgc aatggctcaa agcccatatc cagaccggtc accgtcgcgc cttcgcgcgc
     3661 catactctcg gccagaatgc cgccgccaca accgacatcg agcacctttt tgccaaataa
     3721 accgccagca cgctcggcaa tatagcccag acgcagcggg ttaatgcggt gcaacggttt
     3781 gaactcacct tcaagatccc accagcggga ggcgacggct tcaaatttag cgatctcttc
     3841 gtggtctacg ttatggtttt ccggcgattt ttcggcattc attggcactt ctactccgta
     3901 attggcaaga caaacgagta tatcaggcat tggatgtgaa taaagcgtat aggtttacct
     3961 caaactgcgc ggctgtgtta taatttgcga cctttgaatc cgggatacag tagagggata
     4021 gcggttagat gagcgacctt gcgagagaaa ttacaccggt caacattgag gaagagctga
     4081 agagctccta tctggattat gcgatgtcgg tcattgttgg ccgtgcgctg ccagatgtcc
     4141 gagatggcct gaagccggta caccgtcgcg tactttacgc catgaacgta ctaggcaatg
     4201 actggaacaa agcctataaa aaatctgccc gtgtcgttgg tgacgtaatc ggtaaatacc
     4261 atccccatgg tgactcggcg gtttatgaca cgatcgtccg tatggcgcag ccattctcgc
     4321 tgcgttacat gctggtagac ggtcagggta acttcggttc catcgacggc gactctgcgg
     4381 cggcaatgcg ttatacggaa atccgtctgg cgaaaattgc ccatgaactg atggccgatc
     4441 tcgaaaaaga gacggtcgat ttcgttgata actatgacgg cacggaaaaa attcccgacg
     4501 tcatgccaac caaaattcct aacctgctgg tgaacggttc ttccggtatc gccgtaggta
     4561 tggcaaccaa catcccgccg cacaacctga cggaagtcat caacggttgt ctggcgtata
     4621 tcgatgatga agacatcagc attgaagggc tgatggaaca catcccgggg ccggacttcc
     4681 cgacggcggc aatcattaac ggtcgtcgcg gtattgaaga agcttaccgt accggtcgcg
     4741 gcaaggtgta tatccgcgct cgcgcagaag tggaagttga cgccaaaacc ggtcgtgaaa
     4801 ccattatcgt ccacgaaatt ccgtatcagg taaacaaagc gcgcctgatc gagaagattg
     4861 cggaactggt aaaagaaaaa cgcgtggaag gcatcagcgc gctgcgtgac gagtctgaca
     4921 aagacggtat gcgcatcgtg attgaagtga aacgcgatgc ggtcggtgaa gttgtgctca
     4981 acaacctcta ctcccagacc cagttgcagg tttctttcgg tatcaacatg gtggcattgc
     5041 accatggtca gccgaagatc atgaacctga aagacatcat cgcggcgttt gttcgtcacc
     5101 gccgtgaagt ggtgacccgt cgtactattt tcgaactgcg taaagctcgc gatcgtgctc
     5161 atatccttga agcattagcc gtggcgctgg cgaacatcga cccgatcatc gaactgatcc
     5221 gtcatgcgcc gacgcctgca gaagcgaaaa ctgcgctggt tgctaatccg tggcagctgg
     5281 gcaacgttgc cgcgatgctc gaacgtgctg gcgacgatgc tgcgcgtccg gaatggctgg
     5341 agccagagtt cggcgtgcgt gatggtctgt actacctgac cgaacagcaa gctcaggcga
     5401 ttctggatct gcgtttgcag aaactgaccg gccttgagca cgaaaaactg cttggcgaat
     5461 acaaagagct gctggatcag atcgcggaac tgttgcgtat tcttggtagc gccgatcgtt
     5521 tgatggaagt gatccgtgaa gagctggagc tggttcgtga acagttcggt gacaaacgtc
     5581 gtactgaaat caccgccaac agcgcagaca tcaacctgga agatctgatc actcaggaag
     5641 atgtggtagt gacgctctct caccagggct acgttaagta tcagccgctt tctgaatacg
     5701 aagcgcagcg tcgtggcggg aaaggtaaat ctgccgcacg tattaaagaa gaagacttta
     5761 tcgaccgact gctggtggcg aacactcacg accatattct gtgcttctcc agccgtggtc
     5821 gcgtctattc gatgaaagtt tatcagttgc cggaagccac tcgtggcgcg cgcggtcgtc
     5881 cgatcgtcaa cctgctgccg ctggagcagg acgaacgtat cactgcgatc ctgccagtga
     5941 ccgagtttga agaaggcgtg aaagtcttca tggcgaccgc taacggtacc gtgaagaaaa
     6001 ctgtcctcac cgagttcaac cgtctgcgta ccgccggtaa agtggcgatc aaactggttg
     6061 acggcgatga gctgatcggc gttgacctga ccagcggcga agacgaagta atgctgttct
     6121 ccgctgaagg taaagtggtg cgctttaaag agtcttctgt ccgtgcgatg ggctgcaaca
     6181 ccaccggtgt tcgcggtatt cgcttaggtg aaggcgataa agtcgtctct ctgatcgtgc
     6241 ctcgtggcga tggcgcaatc ctcaccgcaa cgcaaaacgg ttacggtaaa cgtaccgcag
     6301 tggcggaata cccaaccaag tcgcgtgcga cgaaaggggt tatctccatc aaggttaccg
     6361 aacgtaacgg tttagttgtt ggcgcggtac aggtagatga ctgcgaccag atcatgatga
     6421 tcaccgatgc cggcacgctg gtacgtactc gcgtttcaga aatcagcatc gtgggccgta
     6481 acacccaggg cgtgatcctc atccgtactg cggaagatga aaacgtagtg ggtctgcaac
     6541 gtgttgctga accggttgac gaggaagatc tcgataccat cgacggcagt gtcgcggaag
     6601 gggacgatga aatcgctccg gaagtggacg ttgacgacga gccagaagaa gaataatttt
     6661 acttcttcat gccaaaaggg agctatctcc cttgtttgaa ttgaaaagtc caggctgcaa
     6721 agtctgggct tttgtcgtat tagggcacgg taaagtttgg ctgtgcccgt aaaaaatggc
     6781 tggctataca caaggaatgt ggcaatgagt ggtgaaaaaa aggcgaaagg ctggcggttc
     6841 tatggtcttg taggttttgg tgcaatagta ctgctttccg ctggcgtctg ggcgttgcaa
     6901 tatgctggca gtgggccaga aaaaacgttg tcgccgctgg tggtgcacaa caatctgcaa
     6961 atcgatctca atgagccgga cctctttctc gacagcgact ctctgagcca gcttcccaaa
     7021 gatctcctca ccattccgtt tctccacgat gttctgagtg aagatttcgt tttctattat
     7081 cagaatcatg ccgatcgtct gggcattgaa ggcagcattc gtcgcattgt ctatgaacac
     7141 gatctcacgc tgaaagataa actcttttcg tcactcttag atca
//
LOCUS       DAJTMK010000185         7164 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTMK010000185 DAJTMK010000000
VERSION     DAJTMK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.189"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..226)
                     /locus_tag="OO135_003525"
     CDS             complement(<1..226)
                     /locus_tag="OO135_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5399231.1"
                     /translation="MKSVFTISASLAISLMQCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKA"
     gene            603..2519
                     /gene="dnaK"
                     /locus_tag="OO135_003526"
     CDS             603..2519
                     /gene="dnaK"
                     /locus_tag="OO135_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5399232.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2608..3723
                     /gene="dnaJ"
                     /locus_tag="OO135_003527"
     CDS             2608..3723
                     /gene="dnaJ"
                     /locus_tag="OO135_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5399233.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGADFSDIFGDVFGDIFGGGRG
                     RQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCH
                     GSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPAGVDTG
                     DRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAALGGEIE
                     VPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLNEKQKQ
                     LLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3827..3979)
                     /gene="mokC"
                     /locus_tag="OO135_003528"
     CDS             complement(3827..3979)
                     /gene="mokC"
                     /locus_tag="OO135_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5399234.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4565..5731
                     /gene="nhaA"
                     /locus_tag="OO135_003529"
     CDS             4565..5731
                     /gene="nhaA"
                     /locus_tag="OO135_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5399235.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEISKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGVSLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5797..6696
                     /gene="nhaR"
                     /locus_tag="OO135_003530"
     CDS             5797..6696
                     /gene="nhaR"
                     /locus_tag="OO135_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5399236.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6734..>7164)
                     /locus_tag="OO135_003531"
     CDS             complement(6734..>7164)
                     /locus_tag="OO135_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5399237.1"
                     /translation="LMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELV
                     FEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSHDGTNEIQYGKSNYYS
                     FQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1827 a         1678 c         1845 g         1814 t
ORIGIN      
        1 cggctttgaa ataaaccgat gcaccgctta actgtaaatc gccatggtcg gcagagagtt
       61 gtatgcgttt cacaatgcga caaacaggaa gtttcagcgc cagatcgttg gtttcgttac
      121 gcggcattgc aatgacgccg aggagtttat ggtcgtttgc ttgcgccgtg cagcactgca
      181 tcaggctaat cgccaggctg gcggaaatcg taaaaacgga tttcataagg attctcttag
      241 tgggaagagg tagagggatg aatacccact agtttactgc tgataaagag aagattcagg
      301 tacgtaatct tttcttttta ttacaatttt ttgatgaatg ccttggctgc gattcattct
      361 ttatatgaat aaaattgctg tcaattttac gtcttgtcct gccatatcgc gaaatttctg
      421 cgcaaaagca caaaaaattt ttgcatctcc cccttgatga cgtgggttac gaccccattt
      481 agtagtcaac cgcagtgagt gagtctgcaa aaaaatgaaa ttgggcagtt gaaaccagac
      541 atttcgcccc tattacagac tcacaaccac atgatgaccg aatatatagt ggagacgttt
      601 agatgggtaa aataattggt atcgacctgg gtactaccaa ctcttgtgta gcgattatgg
      661 atggcaccac tcctcgtgtg ctggagaacg ccgaaggcga tcgcaccacg ccttctatta
      721 ttgcctatac ccaggatggt gaaactctgg ttggtcagcc ggctaaacgt caggcagtga
      781 cgaacccgca aaacaccctg tttgcgatta aacgcctgat tggccgccgc ttccaggacg
      841 aagaagtaca gcgtgatgtt tccatcatgc cgttcaaaat tattgctgct gataacggcg
      901 acgcatgggt cgaagttaaa ggccagaaaa tggcaccgcc gcagatttct gctgaagtgc
      961 tgaaaaaaat gaagaaaacc gctgaagatt acttgggtga accggtaact gaagctgtta
     1021 tcaccgtacc ggcatacttt aacgatgctc agcgtcaggc aaccaaagac gcaggccgta
     1081 tcgctggtct ggaagtaaaa cgtatcatca acgaaccgac cgcagctgcg ctggcttacg
     1141 gtctggacaa aggtactggc aaccgtacta tcgcggttta tgacctaggt ggtggtactt
     1201 tcgatatttc tattatcgaa atcgacgaag ttgatggcga aaaaaccttc gaagttctgg
     1261 ctaccaacgg tgatacccac ctgggtggtg aagacttcga cagccgtctg atcaactacc
     1321 tggttgaaga attcaagaaa gatcagggca ttgacctgcg caacgatccg ctggcaatgc
     1381 agcgcctgaa agaagcggca gaaaaagcga aaatcgaact gtcttccgct cagcagaccg
     1441 acgttaacct gccgtacatc actgcagatg cgaccggtcc gaaacacatg aacatcaaag
     1501 tgactcgtgc gaaactggaa agcctggttg aagatctggt aaaccgttcc attgagccgc
     1561 tgaaagttgc actgcaggac gctggcctgt ccgtatctga tatcgacgac gttatcctcg
     1621 ttggtggtca gactcgtatg ccaatggttc agaagaaagt tgctgagttc tttggtaaag
     1681 agccgcgtaa agacgttaac ccggacgaag ctgtagcaat cggtgctgct gttcagggtg
     1741 gtgttctgac tggtgacgtg aaagacgtac tgctgctgga cgttaccccg ctgtctctgg
     1801 gtatcgaaac catgggcggt gtgatgacga cgctgatcgc gaaaaacacc actatcccga
     1861 ccaagcacag ccaggtgttc tctaccgctg aagacaacca gtctgcggta accatccatg
     1921 tgctgcaggg tgaacgtaaa cgtgcggctg ataacaaatc tctgggtcag ttcaacctgg
     1981 atggtatcaa cccggcaccg cgcggcatgc cgcagatcga agttaccttc gatatcgatg
     2041 ctgacggtat cctgcacgtt tccgcgaaag ataaaaacag cggtaaagag cagaagatca
     2101 ccatcaaggc ttcttctggt ctgaacgaag atgaaatcca gaaaatggta cgcgacgcag
     2161 aagctaacgc cgaagctgac cgtaagtttg aagagctggt acagactcgc aaccagggcg
     2221 accatctgct gcacagcacc cgtaagcagg ttgaagaagc aggcgacaaa ctgccggctg
     2281 acgacaaaac tgctatcgag tctgcactga ctgcactgga aactgctctg aaaggtgaag
     2341 acaaagccgc tatcgaagcg aaaatgcagg agctggcaca ggtttcccag aaactgatgg
     2401 aaatcgccca gcagcaacat gcccagcagc agactgccgg tgctgatgct tctgcaaaca
     2461 acgcgaaaga tgacgatgtt gtcgacgctg aatttgaaga agtcaaagac aaaaaataat
     2521 cgccctataa acgggtaatt atactgacac gggcgaaggg gaatttcctc tccgcccgtg
     2581 cattcatcta ggggcaattt aaaaaagatg gctaagcaag attattacga gattttaggc
     2641 gtttccaaaa cagcggaaga gcgtgaaatc aaaaaggcct acaaacgcct ggccatgaaa
     2701 taccacccgg accgtaacca gggtgacaaa gaggccgagg cgaaatttaa agagatcaag
     2761 gaagcttatg aagttctgac cgactcgcaa aaacgtgcgg catacgatca gtatggtcat
     2821 gctgcgtttg agcaaggcgg catgggcggc ggcgcagact tcagcgatat ttttggtgac
     2881 gttttcggcg atatttttgg cggcggacgt ggtcgtcaac gtgcggcgcg cggtgctgat
     2941 ttacgctata acatggagct caccctcgaa gaagctgtac gtggcgtgac caaagagatc
     3001 cgcattccga ctctggaaga gtgtgacgtt tgccacggta gcggtgcaaa accaggtaca
     3061 cagccgcaga cctgtccgac ctgtcatggt tctggtcagg tgcagatgcg tcagggtttc
     3121 tttgccgtgc agcagacctg tccacactgt cagggccgcg gtacgctgat caaagatccg
     3181 tgcaacaaat gtcatggtca tggtcgtgtt gagcgcagca aaacgctgtc cgttaaaatc
     3241 ccggcagggg tggacactgg agaccgcatc cgtcttgcgg gcgaaggtga agcgggccag
     3301 catggcgcac cggcaggcga tctgtacgtt caggttcagg ttaaacagca cccgattttc
     3361 gagcgtgaag gcaacaacct gtattgcgaa gtcccgatca acttcgctat ggcggcgctg
     3421 ggtggcgaaa tcgaagtacc gacccttgat ggtcgcgtca aactgaaagt gcctggcgaa
     3481 acccagaccg gtaagctttt ccgtatgcgc ggtaaaggcg tcaagtctgt ccgcggtggc
     3541 gcacagggtg atttgctgtg ccgcgttgtc gtcgaaacac cggtaggcct gaacgagaag
     3601 cagaaacagc tgctgcaaga gctgcaagaa agcttcggtg gcccaaccgg cgagcacaac
     3661 agcccgcgct caaagagctt ctttgatggt gtgaagaagt tttttgacga cctgacccgc
     3721 taacctcccc aaaagcctgc ccgtgggcag gcctgggtaa aaatagggtg cgttgaagat
     3781 atgcgagcac ctgtaaagtg gcggggatca ctccccgccg ttgctcttac tcggattcgt
     3841 aagccgtgaa aacagcaacc tccgtctggc cagttcggat gtgaacctca cagaggtctt
     3901 ttctcgttac cagcgccgcc actacggcgg tgatacagat gacgatcagg gcgacaatca
     3961 tcgccttatg ctgcttcatt gctctcttct ccttgacctt acggtcagta agaggcactc
     4021 tacatgtgtt cagcatatag ggggcctcgg gttgatggta aaatatcact cggggctttt
     4081 ctctatctgc cgttcagcta atgcctgaga cagacagcct caagcacccg ccgctattat
     4141 atcgctctct ttaacccatt ttgttttatc gattctaatc ctgaagacgc ctcgcatttt
     4201 tgtggcgtaa ttttttaatg atttaattat ttaactttaa tttatctctt tatcgcaatt
     4261 attgacgaca agctggatta tttttgaaat attgtcctaa caaataacgc cgactgacaa
     4321 gaaattaatt attacttttc ctaattaatc cctcagaaat cctcacctta agctatgatt
     4381 atctgagctt agggtcactc gtcagcgctt acagccgtca aaaactcatc tcaccgctga
     4441 tggcgcaaat tctacaaagg ctcgtaaaaa acgaattatt cctacactat aatctgattt
     4501 taacgatgat tcgtgcgggg taaaatagta aaaacgatct attcacctga aagagaaata
     4561 aaaagtgaaa catctgcatc gattctttag cagtgatgcc tcgggaggca ttattcttat
     4621 cattgccgct atcctggcga tgatgatggc caacagcggc gcaaccagtg gatggtatca
     4681 cgactttctg gagacgccgg ttcagctccg ggttggttca ctcgaaatca gcaaaaacat
     4741 gctgttatgg ataaatgacg cgctgatggc ggtatttttc ctgttagtcg gtctggaagt
     4801 taaacgtgaa ctgatgcaag gatcgctagc cagcttacgc caggccgcat ttccagttat
     4861 cgccgctatt ggtgggatga ttgtgccggc attactctat ctggccttta actatgccga
     4921 tccgattacc cgcgaagggt gggcgatccc ggcggctact gacattgcct ttgcacttgg
     4981 tgtgctggcg ctgttgggaa gtcgtgttcc gttagcactg aagatctttt tgatggctct
     5041 ggctattatc gacgatcttg gggccatcat tatcatcgca ttgttctaca ctaatgactt
     5101 atcgatggcc tctcttggcg tcgcggctgt agcaattgcg gtactcgcgg tattgaatct
     5161 gtgtggtgta cgccgcacgg gcgtctatat tctggttggc gtagtgttgt ggactgcggt
     5221 gttgaaatcg ggggttcacg caaccttggc gggggtaatt gtcggcttct ttattccttt
     5281 gaaagagaag catgggcgtt ctccagcgaa gcgactggag catgtgttac acccgtgggt
     5341 ggcgtatctg attttgccgc tgtttgcatt tgctaatgct ggcgtttcac tgcaaggcgt
     5401 cacgctggat ggcttgacct ccattctgcc attggggatc atcgctggct tgctgattgg
     5461 caaaccgctg ggggttagtc tgttctgctg gttggcgctg cgtttgaaac tggcgcatct
     5521 gcctgaggga acgacttatc agcaaattat ggcggtgggg atcctgtgcg gtatcggttt
     5581 tactatgtct atctttattg ccagcctggc ctttggtagc gtagatccag aactgattaa
     5641 ctgggcgaaa ctcggtatcc tggtcggttc tatctcttcg gcggtaattg gatacagctg
     5701 gttacgcgtt cgtttgcgtc catcagtgtg acaggacggt ttaccgggga gccataaacg
     5761 gctccctttt cattgttatc agggagagaa atgagcatgt ctcatatcaa ttacaaccac
     5821 ttgtattact tctggcatgt ctataaagaa ggttccgtgg ttggcgcagc ggaggcgctt
     5881 tatttaactc cacaaaccat taccggacag atccgggctc tggaagagcg cctgcaaggc
     5941 aaattattta agcgcaaggg acgtggtctc gaacccagcg agctgggaga actggtctat
     6001 cgctatgccg ataaaatgtt caccttaagc caggaaatgc tggatattgt gaactatcgc
     6061 aaagaatcca atttattgtt tgacgttggc gtggctgatg cactttccaa acgcctggtc
     6121 agtagcgtgc tgaatgctgc agtggtagaa ggcgagccga ttcatcttcg ctgcttcgaa
     6181 tccacccacg aaatgctact ggagcaatta agccagcata aactggatat gatcatttcc
     6241 gactgtccga tagactctac gcagcaggaa ggcctgttct ccgtgagaat tggcgaatgt
     6301 ggcgtgagtt tctggtgtac aaatccacca ccagaaaaac cgttcccggc ttgtctggaa
     6361 gaacggcgac ttttgattcc tgggcgacgt tcaatgttag ggcgcaaatt gcttaactgg
     6421 tttaactccc agggattaaa cgtagaaatc ctcggcgagt ttgatgatgc cgcgttgatg
     6481 aaagcttttg gtgcaatgca caatgcaatc ttcgttgccc caacgcttta tgcatatgac
     6541 ttttatgccg ataaaactgt cgtagaaatt ggtcgcgtcg agaatgtgat ggaagagtac
     6601 catgctattt ttgctgagcg gatgattcag cacccggcgg tacagcgaat ctgcaatacg
     6661 gattattctg cgctttttag tccagcggcg cgttaatcgg cagctccccc aaagttaaag
     6721 tggaggagat agattagttg tacataacca cgattttgac tcggctcatt atttgcccgc
     6781 ttgagacatt atttccatat gtacgcgggc gaataaatag aggaatctga ttacttcctt
     6841 catggggatg ctgaaaagag tagtaattgc ttttaccata ctggatttca ttcgtaccat
     6901 catgggagag ttgaaagcct acttgacccg caccgtcctc actgttttct aactcatttt
     6961 ttaatatgct ggttcctgac tcctgtatcg tcactggttc aaagtaatat tcaacatcac
     7021 tgggttcaaa aactaattca ccttgctcgc cctggatgca gtcaaatctt atatgatgaa
     7081 actgataatt ttcattattg atacgagaca gtacaaatcg tccagtgtcg atggattgat
     7141 cttcaaaacc acaggtcatt aaat
//
LOCUS       DAJTMK010000186         7163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTMK010000186 DAJTMK010000000
VERSION     DAJTMK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.190"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..128
                     /locus_tag="OO135_003532"
     CDS             <1..128
                     /locus_tag="OO135_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR5399238.1"
                     /translation="AILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(182..1297)
                     /gene="rnd"
                     /locus_tag="OO135_003533"
     CDS             complement(182..1297)
                     /gene="rnd"
                     /locus_tag="OO135_003533"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5399239.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPAITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1379..3064)
                     /gene="fadD"
                     /locus_tag="OO135_003534"
     CDS             complement(1379..3064)
                     /gene="fadD"
                     /locus_tag="OO135_003534"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5399240.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDGNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3269..3850)
                     /gene="yeaY"
                     /locus_tag="OO135_003535"
     CDS             complement(3269..3850)
                     /gene="yeaY"
                     /locus_tag="OO135_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5399241.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPAQPI
                     EPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(3890..4585)
                     /gene="tsaB"
                     /locus_tag="OO135_003536"
     CDS             complement(3890..4585)
                     /gene="tsaB"
                     /locus_tag="OO135_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5399242.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(4643..6553)
                     /locus_tag="OO135_003537"
     CDS             complement(4643..6553)
                     /locus_tag="OO135_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5399243.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGELGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6685..7029
                     /locus_tag="OO135_003538"
     CDS             6685..7029
                     /locus_tag="OO135_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5399244.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLV
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         1701 a         1978 c         1780 g         1704 t
ORIGIN      
        1 cagcaatact ggaaggtgaa gctgaccatc gggatcagta ttttagcgat gaagagcgtg
       61 ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa gggtaaacgc ctggtgttgg
      121 atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc ccagtccggc ttcggaagat
      181 tttactgcgg atattcctgc aataaattgt gtaatgcttc cgccatcagc tcaccacgcc
      241 agccggaaat cagctccggc aaattgttct gcggtttcag tttccagtgc cagttcagca
      301 gttggttgat ttgccgacgc gatgccagca attcggcgct gatcttatgc gtttcgctca
      361 cgtcagtaat cagcgactta atcgctttaa acgctttacg ataaccaggc atgtccatca
      421 ggttaagcat cggctgcggt aaggcctctt ccggcaatgt ctgcgctttt tccaccagcg
      481 tcagcagcgt tttaccgtga aagcggattt cgctaccgga taaacccagg ctgtccagtt
      541 cgcctaaact tcccggcata taacgcgcga ccgaccacaa atgctcttca cgcacgacaa
      601 agttcaccgc cagatcacgc tctcgcgcct tgcgcagtcg ccagtcggct aacagttgca
      661 gacaggccag ttggcgtgtg cgtaactgcc aggcattggt gatatcacgc caggcatctt
      721 ccggcgcaac gacttcctga cgacgcattt gcatcaggcg gcattcatcc agcgccgcag
      781 gtagccagcc ggaggcctcc gtttctacca taagcttggc ggtgatcggt aacagatacc
      841 agacatccgc cgctgcgtat tcacactgac gttcggtcag cggtctggcc agccagtcgg
      901 tgcgcgattc actcttgtcc agcgtaacgc cggaatactc ttccaccatg gaagcgaaac
      961 cccatgacat cgggcgtccg cagaaggcag aaaggatttg cgtgtcaatc aagggttgtg
     1021 gtaattcgcc aaagacattg aggaacactt ccagatcttc actgcctgca tggagaaatt
     1081 ttgtgatggc cggatcgcgc aggatcgctt tcagcggtga ccagtcggtg atcccgagag
     1141 gatcaattag cgccagatgc tcgccatcga aaagttgaat caaccccagc tgcgggtaat
     1201 aagtgcgcgt acgaacaaat tcagtatcca gggctatcgc cggaaaggca cggacggctt
     1261 cacacaaaga agccagcgca tcgtccgtgg taatcatttg gtaactcaaa ttgttttctc
     1321 tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc tgacgactga cttaacgctc
     1381 aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc aaaatttttc
     1441 cgacgttaga tttcggtaac tcatcacgaa actccaccag cttaggtact ttgtatcccg
     1501 tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga tcttttttca
     1561 ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca gccgcgactt
     1621 cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag acgttaaaac
     1681 cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct tcttcatcca
     1741 ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg gtagcatcgg
     1801 gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca cccggttgac
     1861 ctggtggtac ttcattacca tcatcatcca ccagtttggc ttccgtcgac ggcaccggca
     1921 aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc agcggcgcac
     1981 actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc caacgctctg
     2041 ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg gagaaatcca
     2101 gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg ccagtgatag
     2161 cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc gggttagtga
     2221 taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc agggcaaaaa
     2281 tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc aacggcccat
     2341 aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc atcgcgcctt
     2401 tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc ggcaccagtt
     2461 cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat gaaatggcat
     2521 ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa ttgactaccg
     2581 tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc tgaacggcgg
     2641 ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca atcgccgatg
     2701 cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg ttaacgttta
     2761 cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc aataaattag
     2821 gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa cccttgttgc aaataagcgg
     2881 caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc atattcacaa
     2941 acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc agagattgat
     3001 aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc caaaccttct
     3061 tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat gatatacatg
     3121 ccattaacat aatattaact catcatacca gcttgataat tacccaacga aaaggttgcg
     3181 aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc ggaccagccg
     3241 ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt actctcgcgg
     3301 gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga ccgccataaa
     3361 accatggctc aatcggctga gccggcataa tcacctgctg ggttaaatgc caacgtttgt
     3421 aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg ccgtcaaccg
     3481 caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc gggtccagaa
     3541 aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc ggtctggctc
     3601 cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg ttttgtaccg
     3661 caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc gcactcatca
     3721 cccgaactaa atcttgttgc ggcgtgggac tgctgccttt aatggcgtcc ggcacagtga
     3781 cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata acattctttt
     3841 gaaccgccat gatgcgactc ctttttttct cagggcatac tcttaagatt cattctttgc
     3901 ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca tgttccaccg
     3961 ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct tccgtcgctg
     4021 gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga tccggccagg
     4081 cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt cgttcatgaa
     4141 cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg ttttcatcac
     4201 gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc agcacgcggg
     4261 ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag acgccaatca
     4321 tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg cgcacgccgg
     4381 taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg gaagttccgc
     4441 tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca cgagggcaaa
     4501 gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag gcctctgtcg
     4561 cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa aaggcgcgca
     4621 tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa cgaaccgcac
     4681 gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc gcgccgtaag
     4741 ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg gcgtcgcgaa
     4801 tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt acttcatcga
     4861 acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc gggtcatccg
     4921 gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca cgcacgtcca
     4981 ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg acaaattgct
     5041 gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc atggtagcgc
     5101 ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata aaacaacgac
     5161 cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca gaacctggct
     5221 ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg taatcaaacg
     5281 ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg aaatgatgca
     5341 gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc ttttgcgcca
     5401 ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta aaatggcgcg
     5461 aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc cgcttcagcc
     5521 gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa cgccccagtg
     5581 acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa aatgcccgct
     5641 gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctagc tcaccgagtt
     5701 gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt aactgctggg
     5761 tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg agcagttgtc
     5821 gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg tgggcttcgt
     5881 cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct ttaaccacca
     5941 tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc gcttttttac
     6001 gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga cagttgtcgt
     6061 tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg caggtgctga
     6121 tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg cttaagattt
     6181 gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg cagaggtagt
     6241 ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt gagactgttg
     6301 gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag ataatgactt
     6361 tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta ccggttcctg
     6421 cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc gccatctgtc
     6481 gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg tctggtgcaa
     6541 aatcgtccgt cacactaccc cctgttgatt tgaacaggga ttatgtcagg atgagggcgc
     6601 tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc agcacgaaaa
     6661 tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga agcccgctgg
     6721 tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga aaacctcgac
     6781 gccgatgctt ttgagcaaac cgccaacacg ctggtgcaga ttgacgccgt gctggaaaaa
     6841 cagggcagca ataaatcgag cattctggat gccaccattt tcctggctga taaaaacgac
     6901 ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc gccggtgcgc
     6961 tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa gattgtggct
     7021 gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat aatactggtc
     7081 ggttttacat aaatcgacca gagattaaga tattactgtt gatgctcctg ctaattcatt
     7141 atttattttt ataatttcaa ttt
//
LOCUS       DAJTMK010000187         7072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTMK010000187 DAJTMK010000000
VERSION     DAJTMK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.191"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(51..176)
                     /locus_tag="OO135_003539"
                     /pseudo
     CDS             complement(51..176)
                     /locus_tag="OO135_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            complement(481..651)
                     /locus_tag="OO135_003540"
     CDS             complement(481..651)
                     /locus_tag="OO135_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399245.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(766..1034)
                     /locus_tag="OO135_003541"
                     /pseudo
     CDS             complement(766..1034)
                     /locus_tag="OO135_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1091..1759
                     /locus_tag="OO135_003542"
     CDS             1091..1759
                     /locus_tag="OO135_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5399246.1"
                     /translation="MVKSMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1814..2398)
                     /locus_tag="OO135_003543"
     CDS             complement(1814..2398)
                     /locus_tag="OO135_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5399247.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDELFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKMFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(2398..5373)
                     /locus_tag="OO135_003544"
     CDS             complement(2398..5373)
                     /locus_tag="OO135_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399248.1"
                     /translation="MAVLISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDARTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAEAASTKASEASKSAAAAESSKSAAATSASAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNAASSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAADSKTAAALSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEAAVLASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYALYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKNTSSFDYGTKSTN
                     NTGAHTHSLSGSTGSAGDHTHGNGIRWPGGGGSALAFYDGGGFTYVQDSQYQVSPGTS
                     SRRSYYQRIQTQSAGAHTHSLSGTAASSGAHAHTVGIGAHTHSVAIGSHGHTITVNAA
                     GNAENTVKNIAFNYIVRLA"
     gene            complement(5438..6037)
                     /locus_tag="OO135_003545"
     CDS             complement(5438..6037)
                     /locus_tag="OO135_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5399249.1"
                     /translation="MRKLCAVILSAVVWLVAAGMPASAAEHQSTLSAGYLQTHTDMPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAKDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVTYSRVSTFSGDYLRVTDSKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTNSFIVGVGYKF"
     gene            complement(6105..>7072)
                     /locus_tag="OO135_003546"
     CDS             complement(6105..>7072)
                     /locus_tag="OO135_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR5399250.1"
                     /translation="QLAPDLTEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVD
                     TNNNLNSMWAVKLQQMKDGRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNT
                     KPMFVGQGDQIFMNDVFLKRLTAPTITSGGDPPAFSLTPDGRLTAKNADISGNVNANS
                     GTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQ
                     PFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDM
                     PAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATTGITIS"
BASE COUNT         1601 a         1898 c         1662 g         1911 t
ORIGIN      
        1 tgccatgtcc ttattgaccc cgtatattac ggggtcgttt ttgtgcggaa ttaaaaacga
       61 tatcctgctg agaacataaa cacccacgga tcgaggcgta cgctatcatg ttgctgtgca
      121 ccgcccagct tataattggc ggtggtatcg atatccatgt accagactgg taggtttcaa
      181 aaaacacaat aaccaattgt atttgaagca aaaaacaaga ctttgagatg ttttttttgt
      241 accttttttt atgtttttaa ctgtctgatt ttattgaaat cttttttagt tttgcagaag
      301 gaatttttta cttgattgta actttttcgt ttttttgggg gctaattgac gcgcaatgct
      361 gggcggggag cgcacagcca aaaaaaccga aattgaccgc taattactgc caatcatcat
      421 cgctctgatt gagcgtaaat cattcaggtt attcagttct tccttctgct ctcgctgacg
      481 tcaataaatc ccatcattgc gatcaacctc atccttaacc attgatgcga gcagttcttc
      541 cagtttctgc gctgacagct ttacctggtg atcttcagca tcttcccggg ctattgtggt
      601 tcgcgcagtg gctgattttg ctgacatcac cacagggggc agacggacca ttgaaccatg
      661 gcctccggaa caaataaggg caaaaataac aaccactatc gaaagctcac aaactaaccg
      721 cagcacgttc ctgcatacga cgtgtctgcg gcataatccc aatgattact ccctgacagg
      781 atttgcaggc cactcaatat cggtgcagtt gatgtatcaa cacgattcaa caatacccga
      841 tatttattcc atgcctccag caacgatctt tcttcctccg ttgcgatttc cagatctaca
      901 gcatcctgca gtggcgcaat atactcactg aattcctgga tgtagaactg tgtggtggcg
      961 gtcttccagc cattcggctc ctgctgtatc gaagcatacc aggctatttc aatatcgcta
     1021 tgctgcggca gcatttaacc ccttgtaatt catcgccata attgatttaa ttcacaaata
     1081 aaactataac atggtgaaat caatgaaaaa aaacacagat gatggggcta aaatttacac
     1141 accacttacc ctaaagcttt atgactggtg ggttttggga gtatcaaatc ggcttgcatg
     1201 gggatgtcct acaaaggaac accttcttcc acactttctg gaacatgtag gtaacaacca
     1261 tctggatatt ggtgttggaa ctgggtttta ccttactcac gtacctgaga gtagtctgat
     1321 atctttaatg gatttgaacg aagctagcct gaacgcggca tctacaaggg ctggggaatc
     1381 aaaaattaaa cataaaatta gccatgatgt ttttgaacct tatcccgcgg cgttacatgg
     1441 tcaatttgat tccatttcca tgttttacct tcttcactgc ctgcctggaa atatatctac
     1501 aaaaagctgt gtaatacgca atgcggcgca ggccttaact gacgatggaa ctctatacgg
     1561 agccacaatt cttggcgatg gagttgtgca caatagcttc ggtcaaaaac tgatgcgcat
     1621 ttacaatcag aaaggcatct tttcaaacac aaaagattcc gaagaaggct taacacatat
     1681 actctcagag catttcgaga atgttaaaac caaggttcaa ggtactgtag taatgttttc
     1741 cgcttcaggg aaaaaatagc atccaaccgc agcacgttct tgcttaagac gtgctgcggc
     1801 ataatcccaa tgattactcc ctgacagggt tcgtaggcca ctcaatatca ggtgcagttg
     1861 atgtatcaac acggttcagc aacacccgat actttttcca ggcttccagc aatgaggttt
     1921 cttcctccgt tgcaatttcc agatctgcag catcctgaag cggcgcaata tgctcactgg
     1981 ctacctgcat caggttgttt tttgtttctt ccgcctcccg gatccggaac attttttctg
     2041 cttccgtatc cttcacccag gctgtgccgt tccacttctg aaactcccct tccggcgata
     2101 accaggtaac attttccggt aacggaccga gttcagaaat aaataactcg tcccctgacg
     2161 ctacgtcata aaccgtttta ccccgatggt cttcaacgag atgccacgat gcctcatcac
     2221 tgttgaaaac agccacaaag ccagcaggaa tatctggtgg tgcaatatcg gtactgtttg
     2281 ctggcagacc tgtatgaggc ggaatatatg cgtcaccttc accaataaat tcattagttc
     2341 cggccagcag attataaatt tttatggtcc gtgcttgttc actcattctg aatgccatta
     2401 tgcaagcctc acaatatagt taaatgcgat gtttttgacg gtgttttccg cgttaccagc
     2461 agcgttaacg gtgatggtgt gtccatgtga accaatcgca acggagtgcg tatgcgcacc
     2521 aatacctaca gtatgtgcat gtgcgccaga acttgctgca gtaccagaca gcgagtgggt
     2581 atgagcacct gctgactgtg tctgaatacg ttgataatac gatctacggg aagaagtccc
     2641 cgggcttact tgatactgtg aatcctggac ataagtgaac ccaccgccat cataaaatgc
     2701 taacgcagaa ccgccgcctc ctggccaacg aataccatta ccatgagtat gatcaccggc
     2761 agaccccgta gagccactca gactgtgcgt atgcgccccg gtgttattcg tggatttggt
     2821 tccgtaatca aacgacgatg tgtttttcgt ccccaaatcc gtactggatg cgctggcgct
     2881 gtgggtgtgc gatttaatgc cgtcctgttc ctgagacaat acggcacgac cactggcggg
     2941 tttgccctta atcgtccagc cacgcatatc agggatcacg cctgacggat aagcggctgc
     3001 aagtttcggg tatgcagatt tgtcaaaagt ctgcccctgc atcagggcat agccagacgg
     3061 aacggtatct gatggccacg ggattggtgc gccaggcgga taaaactgct ctgatggcgt
     3121 atagagtgca taaactgtac cgtccgttaa cccttccggc ttattagcag aatatgctgg
     3181 tgacgtatga atcgtcacgc tggcattact ggtataatcc cattgaatat ttacaccagt
     3241 cgcataattt ccgattgcaa cgtaaatatc gtaagtatca ccagatgtat tgacccaggc
     3301 aaaatttgta aaccctgtcg atgtgcgctg ccataaagca ccagtaatcc ccttcggatt
     3361 accattacct gcacgcaaaa caagttcaga tatacctgcc tgttgaggtg accccacgtt
     3421 aaatcccgca ccaccaatca acgtaattga aacaacagaa ctcgcctgtg gcatggttac
     3481 cgttgctaat ttgaaccaac cagcaccacc gctgaatgac attgttgttg agttaagcgt
     3541 accaatatct ttcggcgtca gtgttatatc cgctgaaagc gccttaccat tcaccttacg
     3601 ggcagaaggt acccgaccat tcgcattatc attagctgct ttcactgctt tcggtgtcgc
     3661 ggcaagcgtt tcagatgcgc tgttggttgc actactgagc tggacaattc ctttttgtgc
     3721 tgtcgtagcg tcctgagcag tatatttccc gttagcaagg tcataggctg cctttaccga
     3781 ctttggcgtt gccgccagca tttcagatgt gctgttggtc gcgttgctaa gctgaactat
     3841 ccctttctgt gctgtcgttg catcctgtgc ggtgtatttc ccgttagcca gctcatacgc
     3901 ggccttaacg gcttttggcg ttgccgccag tgactcggaa gtgctgttag tcgcactgct
     3961 gagctgtact atcccctttt tcgtcgtgct cgcatcctca agcgccacgg cggatgcaat
     4021 atcctctgcc cgttttgccg ctgtctcagc gcgcgttgcc gcggattccg ccgtactttt
     4081 gctctgagct gccgccgtcg cactgccagc tgcctctgtc gccttcgtgg atgccgtcgt
     4141 ggcgctgccc tttgctgctg acgcctgtct ggtcgcctca tcttttgaag cagacgcaga
     4201 tgatgccgat gacgccgccg aactggcgga cgatgcggca gccgtttttg aggattctgc
     4261 gctggtttcc gacgctttcg cgttcgtctc ggatgtcttc gctgcggaag cagacctcgc
     4321 tgctgcgctg gccagtacag cggcttcgcc agccttcgtt gtggctgttg aagcagacga
     4381 tgcagcactt tctgccgatt ttccggcggc ggtggcactg gctgaggcct gcccggcact
     4441 tgttgacgcg gcactggcag ataatgcagc cgctgttttt gagtctgccg cagctgaggc
     4501 gctctgtccc gctgccgttt cagaagacct ggcgttcgtc tcggacgttt ttgccgcctt
     4561 cgcagaattt gctgccgccg ttgccgagga agctgcgctg ctggcgctcg aggctgcgtt
     4621 cgtttctgat gattttgccg cctcttttga agccgacgca tcccgtgctg aagtggctgc
     4681 ttctgacgct ttcgtggtcg cggtggatgc agaagtggct gctgattgtt gtgacgctgc
     4741 cgcattcgtt tctgacgttt tcgcggcact ggcactggta gctgccgcgc tttttgagga
     4801 ctctgcagca gcagcacttt tcgatgcttc actggccttt gttgatgccg cttctgcgct
     4861 ggaagacgct gactgagccg acgacgcggc ctgtccggct gacgtgctgg ctgcgcgtgc
     4921 tgagcctgca gcatcagtcg catgggttgc cgcctcacgg gctgatgtgc gggcatcgct
     4981 ggctgacttc ttcgcggctg ccgtgttctg tgccactgcg gacgcgttac gcgccacctc
     5041 ttccaccatc agttcaaaac ggcgcagtgc ctctggccgg acatcatcct ccgtcattgc
     5101 accgaggaaa tcattcagtg tgcccggctt tgagtcctca tatacggtga taatcccggc
     5161 gtgtgacggc gggaatccct ccaccaacag actgacgctg tactgaccat actcgacgtc
     5221 cattgtataa cgcccggctt catccggatt ttctgaggcc actgtgttca ccaccaccgt
     5281 ggtgctgttg cgcctggcct ttagctgaat ggtgcagttt tgtatcggct tacctgcacc
     5341 atctttcagt acacctgaaa tcagtactgc catattcccc ccacaaaaaa gcccgcctga
     5401 accggcgggc tgtcataaca ctgtgttacc tggctaatca gaatttatag ccgacaccaa
     5461 cgatgaaact gttggtacgc cagtcaccac tgccggagcc ttcataagca atatcaatgg
     5521 ccacggattc ggtcgggtta aactgcacgc cagcccccca cgccagagac gtgttgctgt
     5581 ggcgaccgtc atcacttccg gtcagcacat cgtgcgtttt ccccttgctg tcagttacac
     5641 ggagataatc cccggaaaaa gtcgacacac gactgtaagt cacacccgcc atcgcatacg
     5701 cgctgaacca ttcattcacg cgcacagacg gccccgccat cacgctgaac cagcggttac
     5761 gcacggaatc ttcatgccag cgggtatcgc tgtaatgcgt tttttgctca tctttggcat
     5821 tggcataact gaatgacgtc accagcccca gcgtgtccgt aaactcataa cggtatttca
     5881 cgttaatccc gttcagatca tcgctgcctg gcatatcagt atgggtctga agatacccgg
     5941 cgcttagtgt ggactgatgc tctgctgcgc tcgctggcat accagcggca accagccaga
     6001 ctactgcgga cagaataaca gcacataatt tacgcataat tacctctcgc ttttctgcaa
     6061 taaaaaaggc accatttctg gtgcccgtat ctgggttata aaattcagct aatcgtgatg
     6121 cctgtagtgg ctttcttcat caccacaacc agcaaatcgc tgatacttgc tgtgggatac
     6181 cagttattta ccagccatgc tgacaccgaa aactccagtg tcatgtgacc gtgaccggca
     6241 ggcatatcaa taacgccact gtaaatcagc gtattatcca gcgcggtacg gttataaatt
     6301 tcagcaccgt ttttccgcac tatcagacgg catgaggagt aaatatcagt atgctctctc
     6361 tcatgcttag cgccactgaa tgccaccgcc ggaataacaa tttgccggtc aaacggctga
     6421 tcgtcataaa ccctgacggt aatggtccct gatggccacc gctccggtgc acgggagtcc
     6481 cgggggaaag ctttgcccac tgttttaacg agatcgcctt caatctggtt ggcggacagt
     6541 tttcccagaa cccgacagtt ctcgttaatc gtgacgttgt tgagcgtccc ggagttcgca
     6601 ttcacgttac cgctaatatc ggcatttttc gccgtcagcc gcccgtccgg tgtcagggaa
     6661 aatgccggag gatcaccgcc gctggtaatg gtgggagccg tcaggcgttt caggaacacg
     6721 tcgttcatga atatctgatc gccctgacca acaaacatcg gctttgtgtt gccattcgca
     6781 ggattaatca tcgcaatcct gtctgccgcc agcagcacct gactctgcat tcctgctggc
     6841 gtattctcaa taccggcacc gatacccgca atataaaggc gtccgtcctt catctgttgc
     6901 agcttcacag cccacatgct gttcagatta ttatttgtat caacctgaac tttctgtatc
     6961 tgctggattg cagcactctg attttccagt tttttattga cggtttgcgt gatttcattg
     7021 ctgacattcg taatggacgt cctgatttca gtcaggtccg gcgcaagctg ac
//
LOCUS       DAJTMK010000188         7050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTMK010000188 DAJTMK010000000
VERSION     DAJTMK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.192"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..97)
                     /locus_tag="OO135_003547"
     CDS             complement(<1..97)
                     /locus_tag="OO135_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5399251.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGV"
     gene            complement(110..1891)
                     /gene="gcl"
                     /locus_tag="OO135_003548"
     CDS             complement(110..1891)
                     /gene="gcl"
                     /locus_tag="OO135_003548"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5399252.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTTGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAVDAPTETCFMHYE"
     gene            complement(1981..2796)
                     /gene="allR"
                     /locus_tag="OO135_003549"
     CDS             complement(1981..2796)
                     /gene="allR"
                     /locus_tag="OO135_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5399253.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(2874..3356)
                     /gene="allA"
                     /locus_tag="OO135_003550"
     CDS             complement(2874..3356)
                     /gene="allA"
                     /locus_tag="OO135_003550"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5399254.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3586..4488
                     /gene="allS"
                     /locus_tag="OO135_003551"
     CDS             3586..4488
                     /gene="allS"
                     /locus_tag="OO135_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5399255.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNIVINNLLYNPQAVAQ
                     LLAWLNERYPFTQFHISRQIYMGVWDSILYEGFSLAIGVTGTEALANTFSLDPLGSVQ
                     WRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDME
                     TKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAVED
                     IVTLFTQRRPEISGFLEIFGNPRS"
     gene            4557..5651
                     /gene="mnmH"
                     /locus_tag="OO135_003552"
     CDS             4557..5651
                     /gene="mnmH"
                     /locus_tag="OO135_003552"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5399256.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            5767..6132
                     /locus_tag="OO135_003553"
                     /pseudo
     CDS             5767..6132
                     /locus_tag="OO135_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(6694..>7050)
                     /locus_tag="OO135_003554"
     CDS             complement(6694..>7050)
                     /locus_tag="OO135_003554"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399257.1"
                     /translation="LYKDESLVSKHYINYMAIPENDGVFTWLPDFFSHVAVDISIYTN
                     VEDDYFFSYFSLTNDEEGRFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGD
                     GVNLFLLVKLMLRIIL"
BASE COUNT         1804 a         1815 c         1688 g         1743 t
ORIGIN      
        1 caacgccgcg aaaaccacac tgcgcagctt tctcaaaacg ggcgagaaaa tcatattcac
       61 caaataacat cgataaatta gcagagaaac gtaacattat ttatctccct tattcatagt
      121 gcatgaagca ggtttcagtc ggtgcgtcca ctgcgttatc ggcgatatct tcaaattcca
      181 tgacgttatc cagttcgctg cccatcgaaa tattggtcac acgctcgaga ataacttcca
      241 cgactaccgg tacccgatat tgcgccatta aggctttcgc ctgttcaaag gctggcgcaa
      301 tatcttccgg tttgaagacg cgaatcgctt tacaacctaa accttccgct acttttacgt
      361 ggtcgacgcc gtaaccgttc acttcgctgg agttgatatt ctcgaaagcg agttgcacgc
      421 agtagtccat gtcaaaggcg cgttgtgact gacgaatcag gccgagatag gcgttattca
      481 ccagcacatg gatgtacgga atgttgaact gcgcgccaac ggctaactct tcaatcagga
      541 actggaagtc aaagtcgcca gaaatcgcca ccacattgcg tttcggatca gcggcacaaa
      601 cccctagcgc agccggaatc gtccagccta acggaccagc ctgaccacag ttgatccagt
      661 ggcggtcttt aaagacatgc agcatttgtg ccgcagcgat ttgtgacaga ccgatggtgg
      721 tgacataaca aacatcgcga ccaaaggctt tgttcatctc ttcatacacg cgctgcggtt
      781 tcaccggcac gttgtcgaag tgggttttgc gcagcaaagt gcgtttgcgc tgctggcagt
      841 cggcgaccca ttctttacga cacggcagac gacccgcttt ttgcatctcc tgcgccactt
      901 caaccagcag tgtcagcgcc gctttagcat cagagacaat gccgagatcc ggacacagca
      961 cgcgaccaat ttgcgtcggc tcaatatcga tatgaacgat tttgcgccct tcggtgtatt
     1021 tctctaccga accggtatga cggttagcaa aacggttacc gataccaaac accatgtcgg
     1081 acgccagcaa cgttgcgtta ccgtaacgat gcgcggtttg cagaccgacc atcccggcca
     1141 tcagttcatg atcgtccggg atacagcccc agcccatcag cgttgggatc accggaacgc
     1201 tggtcagttc agcaaactgt tgtaacagtg cagctgcgtc agcattgatt accccgcccc
     1261 cggcaacaat caccggacgt tcggcctgga ttaacatttc tacagctttt tcgatctgca
     1321 tacggctggc agcaggtttg tagaccggca gcggttcgta catgtcagga tcaaactcga
     1381 tttccgcaac ctgaacgtcg aacggtaaat ccaccagtac cggacccgga cgaccagaac
     1441 gcatcaggtg aaatgcctgt tgcagcacgc gaggcaccag cgccgcttca cgaactgtaa
     1501 ccgccatttt gctgaccggt ttagcaattg cttcaatatc tacggcctga aaatcttctt
     1561 tatgcagacg ggcgcgcggt gcctggccgg taatgcacag aataggaatg gaatcagcag
     1621 aagcggaata gagcgcggtg atcatgtccg tgcccgcagg accggtagtc cccagacata
     1681 cgccgatatt ccctgccgtt gcgcgggtat aaccttccgc catgtgcgaa gcaccttcca
     1741 catgacgcgc cagaatgtga cgaataccgc cgtgcttacg catcgctgag tagaacggat
     1801 tgattgcagc tcccggaaca ccgaaggcgg tggtgatacc ttctttctcc agcacataca
     1861 ttgccgcgtc aacggctctc atttttgcca ttttattcct atctctattc attggaaaat
     1921 ttttccaact ttcaaatttt gtcaaaaaaa cccgctccgc agagcgggat gcgacagaca
     1981 ttatggatgt gctttcagtc ccaacgccgt gctgatatcg cgggcggtgt ctctgaccag
     2041 ctcaccctga ctgacaaaac gatcttctgt cagtcttgat gaaggcccgg agatggagat
     2101 agcggcaaca acgctaccga catcatcgta aattgctgaa gctatgcaat tcagacctac
     2161 aacatgctct tctttatcta cggtatagcc cagttcacgc gcttgttcca ggtccttcag
     2221 caaggtgggc atatccacaa gcgtagttgg cgtaaactgc tgcaaaccgg tttgcagaat
     2281 gatgctcatc aactcctctt ccgccagcgg ataaagcagc gctttgcccg caccggaagc
     2341 atgcagcggc agacgactgc ccagtggcgc acacatcctg accattgatt tacactctaa
     2401 ctgaccaatt aatactgctt cattgccgtt acggatcgcg acattgaccg tttcgccgga
     2461 aagtaacatc aggcggcgca taaacggccc ggcgacggag aggacatcgc ggttatggat
     2521 gtacgccgca ccgacgttaa agacacctaa tcctatatgc caccagccta attgactgtc
     2581 ctgatagaca aaatccgctg cttgtaaaac cttcagcaag cgaaaggtcg tggagagcgg
     2641 caaatccaga ttgagagaaa tatcgctaac cgacgaactt cccccacttt tttccagata
     2701 ttgcagaatc gcaattcccc gctctaacgc ctgtgcgccc ttctgtgcca caggctccgc
     2761 ctgtcctggc ctgccgcgcc gtctaacttc cgtcatacct acgttaactc cgacctacaa
     2821 gtgagcataa acaatgaaca attagtttat cttatgcaag tcggttgcag gcgtcacgca
     2881 aaacagagtt cctgttcagg aatactttca acatcacagt tgtcactgcc gccgcgatcg
     2941 atggtcagaa aatcggtgac gcgctgccag gcgaaaagtg ggtgatgcca gacgttacga
     3001 tggtaattca ctccctgttc gccgttggtg ataaacgccc gcagcgttga caggtctggt
     3061 ttgtcgtcgc ctaacgccac gaccaccaca aacacttcac ctttcatcgg gataaaggcc
     3121 tgagtgccca gcggatgacg ttcgagttcg tgaatggtca gcggcagatt cgccggttgc
     3181 gcgcggttaa tgctaataag cgtacggtct tgctcaagaa tctcaaccag cgccaaatcg
     3241 tggtaacgct ccaccaggcc attgttaata tggaaaaaat cccgttgctg agtttcgatt
     3301 acgtctccat aagcactaaa ggcttcctga cttaacggta atacctgaag tttcatcttt
     3361 ttgtccttgt aacataattc cgttcaacca ccgccgaccg gtcagcttgg tttcaataac
     3421 aggcaatcta aaaccttaag agaacaacat caatacaaaa gttgaaatat ctttccaaaa
     3481 ctagatttag tccacaaatt gaacagggtt atcatgtaaa aatactgata aatcagagga
     3541 taagaaaatc acgcaacttg cggatttaaa aggggatacc tgaagatgtt cgatccagaa
     3601 accttgcgga ctttcattgc ggttgctgaa acaggaagtt tttcaaaagc ggcagaacga
     3661 ttatgtaaaa ccacggcgac gatcagttat cgcattaaac ttctggaaga gaataccgga
     3721 gtagcgctgt ttttccgtac gactcgtagc gtaacgttga cagcggctgg cgagcatcta
     3781 ctttcccagg ccagagactg gctgagctgg ctggaaagta tgcccagcga gctgcaacag
     3841 gtgaatattg tcatcaacaa cctgctctat aacccccagg ccgtcgccca gttgctggcg
     3901 tggctgaatg aacgttaccc ctttacccag tttcacatct cccgacaaat ctatatgggc
     3961 gtctgggact cgatattgta cgaaggtttt tcgctggcta tcggcgtcac gggaactgag
     4021 gcgttggcaa atacctttag tcttgatccc ttaggatcgg tgcaatggcg atttgtcatg
     4081 gcggcggatc atccgctggc gaacgttgaa gagccgctaa cagaagcgca gttgcgtcgc
     4141 tttccggcgg tcaatattga agacagcgcc cgcaccttaa ccaaacgcgt cgcctggcga
     4201 ttgccagggc aaaaagagat tattgttcct gatatggaaa cgaaaatcgc cgcccatctg
     4261 gcgggcgttg gcattggttt tttgccaaaa tcgctttgcc agtcaatgat cgataatcaa
     4321 caactggtca gccgggtaat cccaacgatg cgccctcctt cgccattgag tctggcatgg
     4381 cgcaaatttg gcagcggcaa agcggtagaa gatattgtga ccttgtttac ccagcgcagg
     4441 ccggaaatca gcggattttt agaaattttc ggcaacccac gcagttaaaa atcaagtttc
     4501 cttgcgcgtt ggcgcgtaaa atagcgcgca agctgatttg aggttcactt ttaactatgc
     4561 aagagagaca cacggaacag gactatcgtg ccctgctgat tgctgatacg cccattattg
     4621 atgttcgcgc ccctatcgag tttgagcaag gcgcaatgcc cgccgctatc aatctgccgt
     4681 taatgaataa cgatgaacgc gccgccgttg gcacctgcta taaacagcaa gggtcagacg
     4741 cggcgctggc gctggggcat aaactggtgg cgggtgaaat tcgtcagcag cgcatagacg
     4801 cctggcgggc agcgtgcctg caaaatccgc aaggtattct ctgctgtgcc cgtggcggtc
     4861 aacgctcaca tattgtgcaa agctggttgc atgcagcggg gattgattat ccgctggtgg
     4921 aaggcggtta taaggcactg cgccagaccg cgattcaggc tactattgaa ctggcacaaa
     4981 aaccgatagt gctgattggc ggttgtaccg gcagcggtaa aacgctgtta gtgcagcaac
     5041 agccgaacgg tgtcgatctg gaagggctgg cgcgtcatcg cggttcggcg tttggtcgca
     5101 cgttacaacc acaacttagc caggcgagtt ttgaaaatct gctggctgcc gaaatgctaa
     5161 aaaccgacgc ccgtcaggat ttgcgcctgt gggtgctgga agatgaaagc cggatgatcg
     5221 gttcgaatca cctgccggaa tgtctgcgtg agcgaatgac tcaggcggcg attgcggtgg
     5281 tagaagatcc gtttgagatc cgtcttgaac gcctgaacga agagtatttc ttgcgtatgc
     5341 atcatgattt tacccacgcg tacggcgacg aacagggctg gcaggaatat tgcgaatacc
     5401 tgcatcacgg actttcggct attaagcgtc ggctggggtt acagcgctat aacgaactcg
     5461 ctgcccagct ggatacggcg ctgacaacgc aactcaccac cggcagcacc gacggtcatc
     5521 tggcctggct ggtgccgtta cttaaagaat attacgaccc aatgtatcgc tatcagcttg
     5581 agaaaaaagc ggaaaaagtt gtctttcgcg gtgagtgggc ggaagtggcg gaatgggtta
     5641 aggcgcggta atgagggacg tatttgtatt agtcgcgact tgatctgaca catggccttg
     5701 aaaggttgag agttaccggt tttgatatga gtatcgaatc cttatccaaa acaagaggta
     5761 actctcatgc ttcatactgc caatcccgtc atcaaacaca aagccggttt gctcaatctg
     5821 gctgaagaac tcagcaacgt atcaaaagcc tgtaaaatca tgggcgtttc gcgtgatacg
     5881 ttttatcgtt accgcgaact ggtcgctgaa ggcggtgtgg atgcgcagat taatcgtagt
     5941 cgccgcgctc ctaaccttaa gaaccgtacc gatgaggcaa ctgaacaggc tgttgttgat
     6001 tacgccgtcg ctttcccgac acatggtcag caccgggcca gcaacgagct gcgtaaacag
     6061 gggggtttta tctccgacag tggtgtccgt tctgtctggt tactccataa ccttgagaat
     6121 ctcaaaagac gatactgaac aaattttatc aggtgacgtt ccgtaaaaag ttgtatggag
     6181 ttctcgatgc agtcggcgcg actatcagta cccccgctga accctggact atcgggccgc
     6241 cgtactgggg catgtcaccc tggcgtgccg ctggttttcc gctaatggtt tatttcctct
     6301 ttctttcccg gcaagaattt attttaatcc ggaaggatta tttattttca gatagggact
     6361 cacattaaac acgtaacatt ttaattaatg catggctgaa tatgtattac acaatgtcag
     6421 atatcgtaaa agaccagtgc aattctatgt aaactctctg actgttcatt ttatttgttg
     6481 tttcagggtc ggaactcacc ctcaataaac aactgaatac gagtatatat tcatcgtctg
     6541 agctatatgg ctttacacaa tagccgacat gtcttttctc agtcaggaat tttacttttc
     6601 ctcactacga agttgacgac ttcgatatgg gatagactct taattcaagc aattactctg
     6661 cacaagcatc aggcagtcca gctccaatat ttgttataaa ataatgcgta acattaattt
     6721 caccaacaaa aaaaggttta caccatcccc ttgccctggt atcttaccat atttacacca
     6781 cacttttttt gtatctggat cattctttga tacgattttc gccacttgtt ccctggccct
     6841 cactgtcaat gttttcttaa acctaccctc ctcatcattt gttagggaaa aataagaaaa
     6901 aaaataatca tcttctacat ttgtgtatat tgatatatcc accgctacgt gcgaaaaaaa
     6961 atctgggagc catgtaaaaa ctccatcatt ttctggtatt gccatatagt ttatgtaatg
     7021 tttacttaca agagattcat ctttgtataa
//
LOCUS       DAJTMK010000189         7014 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTMK010000189 DAJTMK010000000
VERSION     DAJTMK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.193"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..371)
                     /locus_tag="OO135_003555"
                     /pseudo
     CDS             complement(1..371)
                     /locus_tag="OO135_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    complement(<1..90)
                     /locus_tag="OO135_003555"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(397..820)
                     /locus_tag="OO135_003556"
                     /pseudo
     CDS             complement(397..820)
                     /locus_tag="OO135_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(1006..4314)
                     /gene="icsA"
                     /locus_tag="OO135_003557"
                     /gene_synonym="virG"
     CDS             complement(1006..4314)
                     /gene="icsA"
                     /locus_tag="OO135_003557"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5399258.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEVISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGDMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4843..6045
                     /gene="virA"
                     /locus_tag="OO135_003558"
     CDS             4843..6045
                     /gene="virA"
                     /locus_tag="OO135_003558"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5399259.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPSDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            6224..7014
                     /locus_tag="OO135_003559"
                     /pseudo
     CDS             6224..7014
                     /locus_tag="OO135_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    6504..6620
                     /locus_tag="OO135_003559"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2071 a         1633 c         1299 g         2011 t
ORIGIN      
        1 cggtccgacc ccgtactgct cacgcagctt atccagcagt ggcatcattt tttccagagg
       61 cggtcgaact tcgccttcgc aaaataagcg gaagcctggc gaaggatatc gttactgcgg
      121 cgcagttcac gattttcacg ctccagctct ttcagacgct gacgttcagc ggtggtgagc
      181 cctccatcac cgcccccggt atcccgctca tgctggcgaa cccagacacg cagagtctcc
      241 ggcgtacagc caatctttgg agcaatggaa caaattgtcg cccattgtga gtcatattcg
      301 ccctgacttt ccagaaccat acggactgcc cgttgacgga cttcggggga aaaacgagta
      361 tttttagtca tcctgtttac ctctttctca ggaagtttag tctccaggat tcccggggcg
      421 gttcagttca ggctgtacgc ttccccatgt gatttgcctc gccacttcac tgggctcagg
      481 accccgtagg atcttcaccg aaaaaaatgc ttcaccagtt gctttattat ttgttttagg
      541 tagacctgtt actaatttat acctactctg accaagtctg gataagaaag gcacacctgc
      601 acatttctac ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata
      661 ttcctcgtac agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt
      721 catatattac tcgaccttgt tgagatacaa caacacgtgc attagtttct gcaataccag
      781 taatctgagg cgcataacca cgaatatttg gcggtagcat tcgttcatcg ctttctaatg
      841 caattcctgt gtaacgccaa gcaacagcgc attctggcgc atgatctggt aaacacgttt
      901 ggcattgatc gcaggcatac catcatgtgc acaaaaaccc accgagcttt tacacggtgg
      961 gtcccagaga aatgcaggac atcaacacgc cctgcatttt tattatcaga aggtatattt
     1021 cacacccaaa ataccttggg tgtctctgta actgttatta cccaattgct gagatacgtt
     1081 tccccagatg cttaggttat ctgcaagacg cccccccaca ccagttttga gttcaatcac
     1141 attacggttg cctatctggt gatttgacat gccattcata attacaccat attgctttga
     1201 gctccatttc caattcactt ccaaaaatgg ttcgaactta cgaagagtct ccttatcgat
     1261 acaactctga ctaattaagg atgtacgcaa tcccaatttt gcttgaatat tattgacccc
     1321 agaggatgtg accatagtgc cattgtgttc aatatagtcg tttgctttta ccccatgcct
     1381 agttatctgg acttgtggat tgagatacaa tgcgttgtca atattattat gagtagtcca
     1441 gcgtatggtt gggtagatat agccagcttc caaagctcct gttattcctt tgctggaata
     1501 tttctcacca gttaacccat ctcctttgac tgtattatta aaccaaccgt actgcaacca
     1561 agcatccata tatagacctg ttcgttcttt ttcatcctga aaccaagaac tgtacaaacc
     1621 ggcagtataa ccgctcacag ttcctcgtga actataacta gtatacttat tatgagtttt
     1681 gctatcctga tatccataag ctcccataat acccacatgc aagccatcat gatcagtgaa
     1741 gtttgtcttc aaaatatctc caccaatctg ataaaccatc ctgttagctg tggtttttaa
     1801 ttgtctatca gctaaattat gctcattatg tccaccaaca gtacgaatcc acatggtggt
     1861 ttcgctgagc tgacctgtag taggatcaac aatagtgcta ccgtgtcggt catagagaga
     1921 aggactaaac atcgtgttca ttgctatcag gttagcaata taactgccag cttcagggcg
     1981 ccatgtgtga atactacctt cacccaaatt aagtgaagat acaagctgtg aactagctcg
     2041 gcgactactc atttgagtag actcttgatt gctcatctgt tttgattctt gattatccat
     2101 ctgactagtt agataccact tattggtatt cagtccagat acagtgccct gtttcaggcg
     2161 atagtcataa ctaccagcaa caatacggcc tttctgaata aaagcattct tatcagaaga
     2221 atctgtcgaa ataatttgaa caccttcaag agttttatct ccagagcctc cctcatttgt
     2281 cactgcaaca taggtaatac cagacgcatg gcctgtaatt tccagccggt cagttttact
     2341 gttgtcattg ccgagaaccg cgcccaaaga taatattccg ccctttccat gccaattacc
     2401 tttctgaaca taggtctgac cgacatttga cgaactatta ttgagaataa catgaccgtt
     2461 tttctccaga gtcatgtcac ctgtgacaat gacggggtca ggcaaaaaag gggcattaat
     2521 attattgatt agcacagtcc cactatttaa taaagtgtta acagtttgat tcatgcctga
     2581 aaaattcaag gtagcacctt tattaacaat aacaccagcg gtacgtgtca tagcttcaac
     2641 tgtccccatt tttagaatgc ctgcattgat gttggtgaaa ccagagtagg tatttttttc
     2701 cgccaaaatg agagttccct cccctgattt tgttagggat tttccgtccc agctatttat
     2761 attatgatgg ctgagattat ctgccagaat agtactaaca gtaagttcag cgttttcttt
     2821 cagattgaac tctccatgac tgtcgccatc tgtatcattc cagctcaaac cgtagatcaa
     2881 tttattctgg tcactgatat agctttttgt atagtcaata gcaccagtag tgagtaacgg
     2941 attgatgata gatatattag aaacactgaa acctgttatc cctttatctg cacttagcag
     3001 tttatagtcc ttctgcgccc agttatttat aatagcctcc acttttacat tgttgccaaa
     3061 tgacatattt ccagaagctt ccagactact gattccagct aaatttaaag aactgttctc
     3121 accaaaagaa acgtcacctg aaacagtgaa tttgttacct gatagtgaaa cagttgtaca
     3181 atcaccagca cataaatcac cttcaatagt tggtgtggtc ccagtgtaat cattgttgat
     3241 aattttcaga atggatgaat tgttaacctg tacatcacca gtaatgacag aaccttcatg
     3301 gagtattaat gaattgtttc caccagtgat gtggatagca ttacctgcta cattttgtgt
     3361 tccatcatct tctttacctg aaattatata tccgctatta gcaatggtca tattgctacc
     3421 atttatagca tccccccaat gtccaccatt accgcctaaa atagctccat tgttgattat
     3481 agacaggtta tctcctgtga tagcattacc accgtagcca tcataaccat aaccgtaagc
     3541 agcacctccg ttgcctccag cgaacgtgcc cttattgatt acagagaggc tgcttcctgt
     3601 gatggcatca cctccattac cgccatcacc ctcaccataa ttgttacctc cattaccacc
     3661 ggaaatagtc cctttgttga ttatgggtag attgactcct gtgacggcat cacctccatt
     3721 ccccccatca ctatcactat aactatcgcc accatgtcca cctgaaataa cttctccatt
     3781 tattataaac agattgtctc ctgtaacagc ctcaccacca tcaccgttat ggtcagcacc
     3841 gctaccgccg ctaccaccaa gaatcatgcc ttgattgatt atagacaagt cagatccggt
     3901 aatagagtca ccaccattac cgccacaact attaccatca ttattatcac cagcaccacc
     3961 atgacctgcg ctaatcataa tatcagagtt aatccgtagc ttagttctag atgcatgaga
     4021 ggggttggaa gaagttatat accacgcatc cattccatct tcaccagctc ccaagggaga
     4081 aagtccatca acaggtgtta ataatttttc ataattgtcc tctgaaaaat gaagttcttg
     4141 agtacccgaa agaggaatag caaaagctat tggccccccg agcaacaggg atgcaccaac
     4201 aacaaaagga gaaaaagaca atttgcatgt tttttccttt accgtaggta attctccggc
     4261 ccccccctgt gaacattggg tcatattaca aaaaaatttg tgaatttgat tcatgcacta
     4321 tattatcagt aagtggttga taaaccccta aaaagattcc gttttatccg gaataaaggg
     4381 acgatatatg caaaaacata ttaaacaaag cctcaaccaa aacatgagag ctgtgttata
     4441 tatcaatgca acgggagaaa tttacctgtg tcttgctttg aaaaaaagag ggggaaagct
     4501 atatgttcga taataaatta ataattttac tatgatagtc tgcttcagac cgcaactttt
     4561 cagttctttc tgtacgcttg cccaaagttt aaccactttg tttttctata cacccaatgg
     4621 ttctatctgc cccttgatgt taatccccaa agccaataac atgaatgtat tcatatcaca
     4681 actttcctac cagactctcc aatgcaataa ctgcataatt gtttttagtg gaaatgtatc
     4741 gttttcttaa agagaagaat aacattccat tttattatgt tcccaattta attatattaa
     4801 tatatattgg catcaatagg aaaatacatc aggagaaatc aaatgcagac atcaaacata
     4861 actaaccatg aaagaaatga ctcatcatgg atgtcaactg tcaaatcaac tacagaagta
     4921 agttggaata aattgagttt ttgtgacgtt ttactgaaaa taataacttt cggaatatat
     4981 tctccacacg aaactctagc cgaaaaatat agcgaaaaaa aactaatgga tagtttctca
     5041 ccatcactat cacaagataa aatggacgga gagtttgccc acgcaaacat agatggcatt
     5101 tcaatacggc tatgccttaa caaaggcata tgttcggttt tttatcttga tggagataaa
     5161 atacaatcca ctcaactttc atcaaaagaa tataataact tgttaagttc actccccccc
     5221 aaacaattca acttaggaaa agtacatact attacagctc cggtatcagg gaattttaaa
     5281 actcacaagc ctgcaccaga agttattgaa acagccatta attgctgtac atcgataata
     5341 ccaagcgatg attacttccc cgttaaagac actgatttta atagtgtttg gcatgatatt
     5401 tacagagata ttcgagcatc tgacagcaat agtacaaaaa tatatttcaa caatattgaa
     5461 ataccactaa aattaattgc agatctaatt aatgagttag gaattaatga attcattgat
     5521 agcaaaaaag agcttcaaat gctaagttac aaccaagtaa ataaaataat caattcaaac
     5581 ttcccccaac aagacctatg ttttcaaaca gaaaaacttt tgtttacttc actctttcaa
     5641 gaccctgcat ttatatccgc actaacatct gcattctggc aatctcttca catcacgtct
     5701 tcctctgtgg aacacatata tgcccaaatc atgtccgaaa acatagaaaa caggcttaat
     5761 ttcatgcctg aacaacgagt tattaacaat tgtggacata ttataaaaat caacgcagtt
     5821 gtccctaaaa acgacactgc aatctctgcc tctgggggga gggcttacga agttagctca
     5881 tcaatattac catcgcacat aacatgtaat ggtgttggta ttaataaaat agaaacaagc
     5941 tatctcgttc atgctggaac acttccaagc agtgaagggc ttcgcaatgc tatccctcca
     6001 gaaagtcgtc aagtatcatt tgccatcata tctcctgatg tttaagccaa tattatattg
     6061 gctgccagac tgtaaaaaaa cgttcttcag aacagctcta taaactatta aaccaaagct
     6121 aaaaggatgg ggaccaatag aatctcagcc catccttttt atatccttat gaaccgcccc
     6181 ggaaatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatactcg
     6241 tttttccccc gaagtccgtc aacgggcagt ccgtatggtt ctggaaagtc agggcgaata
     6301 tgactcacaa tgggcgacaa tttgttccat tgctccaaag attggctgta tactggagac
     6361 tctgcgtgtc tgggtacgcc agcatgagcg ggataccggg ggcggtgagg tgggctcacc
     6421 accgctgaac gtcagcgtct gaaagagccg gaacgtgaaa atcgtgaact gcgccgcagt
     6481 aacaatatcc ttcgcctggc ttccgcttat tttgcaaagg cggagttcga ccgcctctgg
     6541 aaaaaataat gccactgctg gataagctgc gtgagcagta cggggtcgga ccggtatgca
     6601 gtgaactgca tattgccccg tcaacgtatt accactgtca gcaacagcga catcatcatg
     6661 ataaacgcag tgcccgtgcg cagctcgatg actggctgaa gaaagagata ctgcgcgtat
     6721 acgatgagaa tcatcaggta tacgctgtgc gtaaagtcag gcatcagttg ttacgggaag
     6781 gtatcagagt ggccagatgc actgtggcac gtctcatggc ggttatgggg cttgccggtg
     6841 ttctccgggg taaaaaggtc cgtacgacca tcagccggaa agccgttgcc gcaggcgacc
     6901 gcgtaaaccg tcagttcgtg gcagaacgac ctgaccagct gtgggtagct gattttactt
     6961 acgtcagcac atggcagggc ttcgtctatg tggcgttcat cattgatgtg tttg
//
LOCUS       DAJTMK010000190         7013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTMK010000190 DAJTMK010000000
VERSION     DAJTMK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.194"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..246
                     /locus_tag="OO135_003560"
     CDS             <1..246
                     /locus_tag="OO135_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399260.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(215..1951)
                     /gene="aceK"
                     /locus_tag="OO135_003561"
     CDS             complement(215..1951)
                     /gene="aceK"
                     /locus_tag="OO135_003561"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5399261.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLMTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCSEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2232..3536)
                     /gene="aceA"
                     /locus_tag="OO135_003562"
                     /pseudo
     CDS             complement(2232..3536)
                     /gene="aceA"
                     /locus_tag="OO135_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3566..5167)
                     /gene="aceB"
                     /locus_tag="OO135_003563"
     CDS             complement(3566..5167)
                     /gene="aceB"
                     /locus_tag="OO135_003563"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5399262.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAVWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5436..6365)
                     /gene="metA"
                     /locus_tag="OO135_003564"
     CDS             complement(5436..6365)
                     /gene="metA"
                     /locus_tag="OO135_003564"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5399263.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6522..6970
                     /locus_tag="OO135_003565"
                     /pseudo
     CDS             6522..6970
                     /locus_tag="OO135_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1687 a         1802 c         1820 g         1704 t
ORIGIN      
        1 catgcacgcc tgtttggatt taccgctgaa gatattatgg atttttggca acacaaagcg
       61 ccacaaaaat actctgcctt tgagttggct tttgaatttg gtcaccgggt tattgctgaa
      121 ttaatcctta atacattaaa taagatggct gaaagctttg gctttacgga taaccctcga
      181 tacattgcgg agaaaaatta tatggaagct ttactcaaaa aagcatctcc ccataccgta
      241 cgctaaatct ttgcctgcgc cgatacgcat aaacatcttc cacatgccct tcacgtatgc
      301 ggttttgtag tgcgcgccag taatcagcgc ggaacaggtc ggcgtgcatc tcttcaaaca
      361 gcggaccaat acgcgggtcg gcgcatagcc agtggcgaaa ctcttccggg aaaacatcgc
      421 ccggcgagac gctgtaccac ggttcgctgg caagttcgtc ttccggatag cgcggcggcg
      481 ggatgtcgcg gaaattcact tccgtcatgt agcaaatttc atcgtaatca taaaaaacca
      541 cacgcccgtg acgggtgaca ccgaagtttt taaacagcat gtcgccagga aaaatgttag
      601 cagcggcaag ctggcgaata gcgttaccgt attcttcaat ggcgtcgcgc aactgctgac
      661 cttccacttg ttccagccag atattgagcg gcaccatccg ccgctcgata tagagatggc
      721 gaattacaat ctgctcacca agatcggtga ttttttccgc tgcttcctgg agcagtaatt
      781 ccattaatgc cggggaaata tgccgcttct ccagcacaaa gttttcaaac tcctgggtgt
      841 ccgccattcg gcccacacgg tcgtgctctt tcaccagttg atagcaggca cgaacgtgag
      901 cggcagacat ctctttctgc ggcgcgaact tgtctttgat gactttgaat acccgatcaa
      961 agcccggcag cgtaaacacc aacatcacca ttccacgaat acccggcgct tcaatgaact
     1021 gctcactaca gccctgtaga taaacgagat attcgcggta gctttcggtt ttggcgtgct
     1081 tctggcagcc gatagccata tacaattcag cagtggtttt acctggcaga atttcccgta
     1141 gccactcgac cagcgctgcg ggcagcggtg cataaaccat aaaataagaa cgcgcaaagc
     1201 caaaaacaat gctcgcttcg gcggtcgtca tcaggcaggt atcaataaat aactcgccgt
     1261 cgtccgtctg gtggatcggc agtaagaagg gcaacgtgcc ggaaggcgtg atcagtttgc
     1321 ccaccagcca ggcggcttta ttgcggtaaa acagttcgtt cgccacctgt aaatgacttt
     1381 ccgcgaggtt gtctgtcccc agcgtttccg tcagatggcg aatgatgtaa tgaatgtcac
     1441 ggcttttatt ctgccagcgc aggcgcagcg gcaggtcgct gataacgcgc atcagtagag
     1501 attcccagcc gtgatcgggg tgaaagtctt tcgccagcgg gcggggaatg gtacgaaagc
     1561 ggcgctctgg ctgagagcta aagataaaaa gccgctcggg agtaagcgag cggtggtcaa
     1621 ataaccgaca gtacacggag ttaaaaaagc tctccgcaat ctcgaagcgc gggtaatccg
     1681 gcaacagccg cgtgtaatgc tctttaacgc gcagtaaaaa tgccgcgtcc gtactttggc
     1741 cgttagtaat gcagcgcagt tgctccacga ccagaccaac gtgatgatcg taaagatgga
     1801 tacggttttt catcgccagc tggacagcat gccagtcggc ctgttcgaaa cgctgctgcg
     1861 ccccggaagt cacttcgagg aatcgaccat actgagcgtc gaagccttgc aaaatggttt
     1921 gagcaatcag taattccagg ccacgcggca tttaacgccc tcatcaggag cagagaattg
     1981 ccggatgcgg cgtaaacgcc ttatccagcc tacgttcggc aacggttgta ggcatgataa
     2041 gacgcgcaag cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg taaatgcctt
     2101 atccggccta cgttcggcaa cggctgtagg catgataaga cgcgcaagcg tcgcatcagg
     2161 catcgtgcac cgattgccgg atgcggcgtg aacgccttat ccggcctaca gtcagcaacg
     2221 gttgttgttg cttagaactg cgattcttca gtggagccgg tcagcgcggt gactgaagac
     2281 gtgccgccct gaataatagt cgtcacttta tcgaagtaac ctgtacccac ttcctgctgg
     2341 tgagatacga aggtatagcc atctttcgcg gcggcaaatt ccggctgctg cactttctca
     2401 acgtagtgct tcataccctc gccctaggca taggcgtttg ccaggtcaaa catgttgaac
     2461 cacatgctgt ggatacctgc cagggtgatg aactggaact tgtagcccat atccgacagc
     2521 tgctgctgga agctgtcaat agttttgtcg tcgaggtttt tctgccagtt gaacgacggc
     2581 gagcagttat aagccagcag tttgcccgga tatttcgcgt ggatagcttg tgcaaagcga
     2641 cgcgccagtt ccagatccgg cgtggaggtt tcacaccaga ccaggtcagc atatggcgca
     2701 tacgccaggc tacggctgat cgcttgctca atgcccgcat gagtacggaa gaagccttca
     2761 ctggtacgct cgccggtaat aaattcgctg tcatacgggt cgcaatcgga ggtgatcaga
     2821 tccgctgcat cggcatcggt acgagcaacc agtagggttg gaacgcccgt cacgtcagct
     2881 gccagacgcg ccgcgaccag tttctgaata gcttcttgag ttggcaccaa aactttgccg
     2941 cccatgtgac cgcatttctt cactgacgcc agctgatctt cgaagtgaac tgccgctgca
     3001 ccggcttcaa tcatcgcttt catcagttca aaggcattca ggacaccgcc aaaaccggct
     3061 tccgcatcgg caacgatcgg caggaagtaa tcgacatagc gcggatcgcc cggctcaatg
     3121 cccgcggacc attggatctg atccgcacgg cggaaagtat tgttgatccg ctccaccaca
     3181 gctggcaccg agtttgccgg atagagcgac tgatccggat acatgctggc cgccaggtta
     3241 gcgtccgccg ctacctgcca tcccgacaga tagactgctt caatacccgc tttcgcctgt
     3301 tgcagcgcct gaccgccagt cagtgcaccg aggctgttga tgtagccttt tttcgactcg
     3361 ccatgcagca gacgccacat tctcgccgcg cccagttgcg ccagggtgca ttcaggattg
     3421 actgaaccgc gtaatttcac cacatcttcc gcgctgtatg ggcgagtaat gccttcccaa
     3481 cgcggttgag tccactcttt ctgtaattct tcaatttgtt gtgtacgggt tttcatgtgc
     3541 agatgctcca tagttatgtg gtggtttacg ctaacaggca gtagcctggc agggtcagga
     3601 aatcaattaa ctcatcggaa gtggtgatct gttccatcaa gcgtgcggca tcgtcaaaac
     3661 gcccctggga gaaacgttct tcgcccagtt cgctggcaat gactttcatc tcttcgccca
     3721 gcatctggcg gaacaaggct ttagttaccg gtttgccatt gctcaacatt ttttgatgat
     3781 ggatccactg ccagattgag gtacgggaaa tttcagccgt cgccgcatct tccatcaggc
     3841 cataaatcgg cacacagccg ttgccagaga tccacgcttc gatgtactgc acggctacgc
     3901 gaatatttgc gcgcatacct tcttcggtgc gttcaccgtc acaaggtgcc agtagctgat
     3961 cggcagtaat cggcgcgtct tgttcgcgca tcacttcaag ctgattttta cgggagccga
     4021 gaatgtcgtt gaataccgcc attgccgtat ccgcaaggcc tgggtgagcg atccatgtgc
     4081 catcgtgacc gttattggct tccagcgatt tatccgcttt tactttgttg agcacctggt
     4141 tattgtgctc ttcatctttg ctcggaataa acgccgccat gccgcccatc gcaaaagcac
     4201 cgcgtttatg gcaggtttta atcaacaggc gtgagtaagc attcaggaat ggtttatcca
     4261 tcgtcactgc ctgtctgtct ggcaggacgc gatcgggata gtttttcaac gttttgatat
     4321 agctgaagat gtaatcccaa cgaccgcagt tcagaccaac aatatggtca cgcagcgcgt
     4381 gaaggatttc atccatctgg aacacggcgg gcagcgtttc aatcagcaac gtcgccttga
     4441 tggtgccgcg cggcagatta aagcgatctt ctgcatagct gaagacttcg ctccaccaga
     4501 ccgcttcctg ccaggactgg gttttcggca gatagaaata gggaccactg ccctttgcca
     4561 acagagcctg atagttgtgg aagaaataga gcgcaaaatc aaacaggctg ccggggattg
     4621 cctcaccacg ccaggtgaca tgtttttccg gcaagtgcag accgcgtacc cgacaaatca
     4681 aaaccgctgg attgggcttg agctggtaaa ttttgcctgc ttcattggta tagctgatgg
     4741 taccgttaac tgcatcacgc aggttaattt gcccgtcgat cactttgttc cagtccgggg
     4801 ccagtgaatc ttcgaaatcg gccataaaga ctttcacatt ggcgttgagc gcgttgatca
     4861 ccatcttgcg ctctaccggg ccagttatct ctacgcggcg gtcttctaag tccgcaggaa
     4921 tcccgcgaat tttccaatca gcatcgcaaa tggaagctgt ttccgaaata aaatcaggca
     4981 acgttccgtt atcaatatct tgctgctgct gaatgcgcgc tgccagaagt ttatttcgtt
     5041 gtggcgtaaa atgcgtcacc agctcagtca gaaattctac cgcttcggca gtaagaattt
     5101 gcttctcctg ctcgccatac ggccttgtga aagccagttc atcggttgtt gttgcctgtt
     5161 cagtcatcgt gcagctcctc gtcatggatc cgaaaacttc cccactgtga acgaaggatc
     5221 gttgtgcgct tttcgttcag cacaactaag actactcatt taaaatgcaa aatcaaaaac
     5281 aatttccatt ttaattaaaa atacttgata acttattgat aacaatgtga ataaaattta
     5341 ttcacctcct gaggtgcgtt tcggaaataa aaaaggcacc cgaaggtgcc tgaggtaagg
     5401 tgctgaatcg cttaacgatc gactatcaca gaagattaat ccagcgttgg attcatgtgc
     5461 cgtagatcgt atggcgtgat ctgatagacg taatagttga gccagttggt aaacagcaaa
     5521 ttaccgtgac tacgccagct cgctcgcggt gtattttgcg gatcattgtg cgggaaatag
     5581 ttatacggta cttccgggtc tagtccggct tccacatcgc ggaaatattc ctgcgccagc
     5641 gtttgcgcat catattcggg atggcccgtc acaaaggcaa tgcgcttatc tttgctggca
     5701 aacagatatg catccccttc ttccgtctct gccagaattt ccagatcggt gtaatcacga
     5761 atcaacgctg ccggaaagtc agcatagcgc gaatgtggtg ccaggaatga atcatcaaag
     5821 ccacgcgtca gaagcgcatg aggatggaga atatgatgct cgtaaacgcc agagagtttg
     5881 tcggtgcgag tttgcttagg aatgccgtag aggatattga gcgcggcctg taccgcccag
     5941 cagacaaaca gcgtcgaggt gacgtgatct ttcgaccact ccagcacctg tttgatctgc
     6001 ggccagtaag cgacatcatt aaactccacc aggcccagcg gcgcaccggt tacaatcaga
     6061 ccgtcaaagt tctgttcctg aatatcttca aagttacagt agaagttgtt cagatgctct
     6121 gcgggcgtgt tgcgcgattc acgggaatcg atgcgcaaca gctgaatatc gacctgcaaa
     6181 ggtgagtttg aaagcaggcg cagaaactga ttttcagttt caatcttctt cggcatcagg
     6241 ttaaggatca gaaccttaag tggacgaatt tcctgaccag acgcacgaga agttgtcatc
     6301 acaaagacgt tttcttcacg caagaaattg acggcgggta gctcgtccgg cacacgaatc
     6361 ggcataacct gattacctca ctacatacac ttatacgttt ggacatccag atagctgaag
     6421 ataaccagaa aatttgccaa tgtcgagcct gcatgttgaa ggtgagaaag tttcaaggaa
     6481 aagactacgt tagaatataa gaatgactaa aaggagagaa aatggttatt agtattcgcc
     6541 gctcacggca tgaggaaggg gaggaactcg ttgcgatttg gtgtcgatct gtcgatgcca
     6601 cacacgattt tttatcagca gagtatcggg ccgagctgga ggtgctagct agttcgttcc
     6661 ttactgccgg aagcgccgtt gtgggtcgcg gttaatgaac gggatcagcc ggttgggttt
     6721 atgttgttaa gtgggcagca tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc
     6781 ggcgtgggtc ggatgctggt ggagcatgcg ctctcgatgg caccggaact gacaaccaac
     6841 gttaatgagc aaaatgagca ggcggttggg ttctataaga aggtgggttt taaggttacg
     6901 ggacgctctg aggtggacga tttggggaaa cccgtatccg ttgctgaatc tggcgtatgt
     6961 gggggaataa gccgcccctg ccgtgcctgc ccaggctgga gaagacgcgc cag
//
LOCUS       DAJTMK010000191         6890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTMK010000191 DAJTMK010000000
VERSION     DAJTMK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6890
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.195"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..906)
                     /gene="preA"
                     /locus_tag="OO135_003566"
     CDS             complement(<1..906)
                     /gene="preA"
                     /locus_tag="OO135_003566"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR5399264.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSH"
     gene            complement(900..2138)
                     /gene="preT"
                     /locus_tag="OO135_003567"
     CDS             complement(900..2138)
                     /gene="preT"
                     /locus_tag="OO135_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5399265.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSAQELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(2332..2571)
                     /locus_tag="OO135_003568"
     CDS             complement(2332..2571)
                     /locus_tag="OO135_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5399266.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNSGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2574..3293)
                     /gene="sanA"
                     /locus_tag="OO135_003569"
     CDS             complement(2574..3293)
                     /gene="sanA"
                     /locus_tag="OO135_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5399267.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3443..4327)
                     /gene="cdd"
                     /locus_tag="OO135_003570"
     CDS             complement(3443..4327)
                     /gene="cdd"
                     /locus_tag="OO135_003570"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5399268.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEITTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4457..5152)
                     /locus_tag="OO135_003571"
     CDS             complement(4457..5152)
                     /locus_tag="OO135_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5399269.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(5149..5547)
                     /locus_tag="OO135_003572"
     CDS             complement(5149..5547)
                     /locus_tag="OO135_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5399270.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5786..6736
                     /gene="dusC"
                     /locus_tag="OO135_003573"
     CDS             5786..6736
                     /gene="dusC"
                     /locus_tag="OO135_003573"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5399271.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSHDIARAIQAIDIDKLR"
BASE COUNT         1776 a         1791 c         1721 g         1602 t
ORIGIN      
        1 atgcgacaac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
       61 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatcct cccaggtttc
      121 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
      181 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
      241 tccgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
      301 ggtgttaatc gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
      361 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
      421 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
      481 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag cttcttgcac
      541 caggcgcgcc agctcctccc attgctgctc attttccccc atgatcgaag cgatcaatac
      601 tttgtccggg taatcttcct tcagccgacg cagggcggcc agattctctt ccaacggatg
      661 ttcagcaatc tgctccatat ttttgaagcc gataaaaccg gtatcttctt tcaccagatg
      721 atcaaaacgc ggcgagactt cgttggcgat aaaaaagccg atcgttttaa acaccacacc
      781 gccccaacct gtgtcgtagg ctttggcaca catctcatag cagttgccta ccggcgaaga
      841 agagaggcag aacgggttgg gaaacttcac gccgcaaaaa gtaatcgaaa gatctttcgt
      901 taacatgagc aagctccctc taaatagtga tgaatcgcct cggcggcttc tttcccggtt
      961 ttcacggcat agaccacggt tttgtcaccc tcaacaatat cgcccgcagc aaagacttgc
     1021 ggatcgcggg tctggtaatt ttgtgtattg atagtgttac gctgcggctc taactccgca
     1081 aaggcatcca gtctggcatg ctgaccgacg gcgagaataa ttttatctgc cgccattgtc
     1141 agttcgcccg gtaaccgtac atgcttaaac gtgactttat tgccttccac ggctactggc
     1201 gtgaatccat cgatgatcga aacgcccagc tcttgggcgc tggtaaactc tttttcgctt
     1261 gccggaaact catctaactc ttcacgcgct acgcaagtta ccgcctgaca gccgagaact
     1321 ttcagcgtgc tggctacgtc catcgcgaca tcaccgccac cgataattaa tgcgctttgt
     1381 ggaatgctga tatcgccttg cgcctgtcgt acacgttgca ggaagtcgac ggcaatctca
     1441 acgtcgctat gctcaaacag cggtagcccg gaaccgcttg ataaccccac ggtgaccagt
     1501 accgcgcggt tttccgcttt cagttgctcc agggtgagtg ttttaccgat ttcgttattg
     1561 cacttaatgg tcacgcccat tttttcaata cgggcgatct ctgcatccag cactgattgc
     1621 ggtaaccgga attgcggaat accgttacgc aaccagccac cggggtgcgc ttctttctca
     1681 taaatcgtga cgtcataacc ctggtttgtc agtgtcacac tggcctgcaa tccggcagga
     1741 cctgcgccaa taatcgcgac tttgccgagc gttttagtac cgggctgata aatttccatc
     1801 ccggtttgtt gttcaaaatc agtaacaaaa cgctgtaagc ggccgatatc aatgggagca
     1861 tcaacaccgg cacgggtaca accgctttga cataatttct ccgtcgggca cactctggca
     1921 caaacggcac cgagggcatt attttcgcga attgtctcgg cagcgccttt aaaattacga
     1981 aagtagattg agcgaataaa tttccccgga tcggtctgtg ctgggcaagc ctgactacag
     2041 ggagcgtcat gacataataa acagcgagag gcttctttaa tcgccagtaa aggcgtaaaa
     2101 gccggagtga gttcatccag ataattttgt tgcggcatgt gatcttcctt aatgctatga
     2161 gtaattaagg cgaacgctaa ataaatattg cgtgctgata ttaaggctat gcgcgtgggg
     2221 aaatttaaac tgtgaaagga ttcacaaata gctgcggttc agaatgttat atccgcagct
     2281 tattgagtga agtaataatt attctcaata attaacagaa aagagaaaat attaacgata
     2341 gaaaataaga taaacaatca tgccgataga taaaattccg aagccgatat atgccaccat
     2401 ataagcgaaa aagagtccgg aattttttgc tcgccagaca tacaccggtt ccggtgcatt
     2461 tttaacccgt ttatcaatcg cgcctgcgcg ccagcctttc catgcttcgc ggaaacaaaa
     2521 tatcgggata aggaaaaaaa cggcaacgac aaaaaactgc tgtacatcca tagctacttt
     2581 ccttgtttct tttgtaattc aagtaactgt tcgggtgtga cggcgggata cccctgcgca
     2641 tcttccggca cctggtgcat agccggaata gggaccagcg gccctaaaaa acgcggttca
     2701 cgtttaaaaa tataaaggtc agccagcgca ccgaaacggg cggcaaattc gcgaatacgt
     2761 actgacagca tatctttcgg tgacggtacg gcataacact gagcctgaat ccccatatgc
     2821 agcgcaataa ataatgcccg ctcacagtgg aaacgttggg tgataataat gaaatcatta
     2881 gtatcgaaaa ctttgcgtgt acgcactatg gaatccagcg tacgaaagcc tgcgtaatcg
     2941 agaacaatat ctgatgggtc gacaccagca gcgattaaat ctttgcgcat ggtcatcggc
     3001 tcattataac tttgcaatgc gttatcgccg ctcagtaata gataatttac cttaccgctg
     3061 ttataggcat taatcgctcc ttgaatgcgg tagcgataat actgattaat tacgccagta
     3121 cgataatatt ttgctgttcc gagcaccaca ccgacctggc ggtaggggag atcctgcaat
     3181 tcgtcgtaga tataaggcgc ggttttccag ctcatccagc gatcgaggcc gagcacagtc
     3241 aacagcagca agccgataag gactaacagg ctgaggaaca cgcgctttaa catgcaggag
     3301 actcgatcta agggaaattt ccatcaggct actttacccg ctggttttgc gcaagaaacg
     3361 gcaaactgtt tgccgggatt ttcgacacca ggagagggct ggcaagccgg acaaggcgtt
     3421 cacgccgcat ccggcaccag gcttaagcga gaagcacgcg gtcgatactg tgacagccga
     3481 gcgctttcag cgttgcggag gtggcatccc actgaatcaa cggcgcatcg gctttttctg
     3541 ccagaaccgc gcgctggata tccgggtaat cataaccctt gagattcaac agaattaacg
     3601 ctccctgcaa cggtggcaga gtcgggttga atgcggcgtt ttcagcgtag ctgccgctga
     3661 aaatacgacc gtctttacat tccagcgcga caccgcttgg cgacttactg taaggcatgt
     3721 gcgaacggtt tgccgccgca atcgccgcct gagaaagcgc atcaccagtc agcgcatagc
     3781 cgtgatcctg ctcgtccatc agcagcgttg taatctccag atctttcggc ccaaaggcat
     3841 ccggcagata gtcacgtagc gcgtgtgcct cgcggcccgg cagatgaata cgcagatcca
     3901 gaccgctgtt cagttcattc ataaactgac ggcagtgacc acaaggcgtg tagttaacgg
     3961 tgatggctgc aagcgctttt tcaccactca accaggcgtg gctgatcgcg ctttgttcgg
     4021 catgaacggt ttgctgcatt gtcgcaccaa taaattccat attggcacca aaataccagg
     4081 ttccgctcac accgcgcgca attgcgccaa cattaaaatt cgacaatggc gtacgcgcac
     4141 aggccgccgc cagcggaagt agtgcgaatg ccagcgcgtc ttcgtccagc cccgttgcgc
     4201 tcttcagcga tgagacttgc tccccggtca acaaagcggg gaagtacttg tctgccagaa
     4261 taggttccag tgcagattgc aagttatccg caagttgggc aaaagcggtt tgaaaacgtg
     4321 gatgcatggc gttgcctcat aatcatgtaa tgggataaca gtttacgaac ccgttatggc
     4381 tttatatgtg atctgaatct cattaattat gcaacgcgtg aaacttacat caaaaatgcg
     4441 cgacgcatcg caaattttag cccattactg ccagaataat cgggaaaagg aacggtgcga
     4501 tcagcgaagt aattatcccg cataacacca gcgctagcga actaaatgca ccttcctgat
     4561 aatccagctc ggcgcagcgc gctgtaccga gggcgtgcga ggcagtcccc attgccagac
     4621 cgcgcgcagc tttggtacga atacgcatcg cattaagcaa tgtatggcca aataccgcgc
     4681 cgagaatgcc gacgaaaatc acgcaaactg cgctgattgc cggaataccg ccaatactgc
     4741 cgccaaccgc cattgcaata ggcgtggtga ctgatttcgg caggattgac gcggcgattt
     4801 ccggtgaagc gcccatcaac aatgccacgg aagtccccgt caccatcgca accacgctgc
     4861 cgataaaaca gatggtgatg atcgatttcc agcgcgcgcg gatctggtgt agctgctcat
     4921 ataaaggata ggccagcgcg acgaccgccg gttgcagcag atcgtttagc acttcgctac
     4981 ctttaaagta actgtcgtaa gagatgccgg tcagcattaa aaaaggaatg atcaccacca
     5041 ttgctaccag caacgggtta agcaggggaa acttataccg tgccgccagt ttgcgggcgg
     5101 caaaaaagac aatcaacgtc agcggtaatg accaccagat attcgccatc attcttctga
     5161 tcctttctga cctacgactt tacgttcgcc gtgtaccagt tgtgaactcc agctcaccac
     5221 cagaaaaacc accagcgtac tgacagcaca ggaaaccact accgggccaa actgcgcgcg
     5281 gagcaaatca aaatattgca tgacgcctac gccaatcggc acaaacaata gcgccatata
     5341 gcgaatcagt acgtagcacc ccggattgac ccattttgcc ggaagaattt gcaaggccag
     5401 caggacaaac aggatcagca tcccgatgat gctgcccgga atggttaccg gtagcaggga
     5461 agcaatgaaa atgcctgcat acaggcaggc ataaatcagg acgaaagcgc gtaaatattg
     5521 ccagataatg ttcagtgtct tactcataat caacacccca gatgagatgg attcatcata
     5581 caattaatcc gtaaaacgtg ctaccgatca cattaagaga tcagcgctta ttttttcgct
     5641 ccccggcatt acgcaatgcg caacgaactc aggcatacat caagcataga gagggattac
     5701 agtttgcagc aatccctctt tgcagacctt taatccgttg accataggcg ctaacatggc
     5761 gcctcatttt tttgcgggtg aagatatgcg tgtgttactg gctccgatgg agggtgtgct
     5821 tgactctctg gtgcgtgaat tgctgaccga agttaacgac tacgatctgt gcatcaccga
     5881 gtttgtccgc gtggtggatc aactgctgcc ggtaaaagtt tttcatcgca tttgccctga
     5941 gctacaaaac gccagccgga caccatccgg tacgctggtg cgcgtgcagt tgttgggtca
     6001 gttcccacaa tggctggcag agaacgccgc ccgtgcggtg gaattaggtt cctggggcgt
     6061 ggatctcaat tgcggctgcc cgtcgaaaac ggtcaacggt agcggtggtg gggcgacgtt
     6121 actcaaagat cctgaactca tctaccaggg tgcaaaagcg atgcgtgaag ctgtaccggc
     6181 gcatttgccc gtcagcgtga aagtgcgtct gggctgggac agcggtgaga agaagtttga
     6241 aatcgccgat gcggttcaac aggctggtgc tacggagctg gtggtgcatg gacggacaaa
     6301 agagcagggt taccgcgcgg agcatattga ctggcaggcg attggcgaga ttcgccagcg
     6361 gctgaatatt ccggtgattg ccaacggcga aatctgggac tggcagagtg cgcaacaatg
     6421 catggcgatc agcggctgcg acgcagtgat gattggtcgc ggggcgctca atattcccaa
     6481 cctgagccgg gtggtaaaat ataacgaacc gcgaatgccg tggccggagg tggttgcttt
     6541 gctgcaaaaa tatacccgtc tggaaaagca gggcgatacc gggttatatc acgtagcgcg
     6601 gattaaacag tggttgagtt atttgcgtaa agaatacgat gaagcaacag aattatttca
     6661 gcatgttcgg gtgttgaata attcccatga tattgcaagg gctattcagg caattgatat
     6721 cgataagctg cgataatagt ggaatcgcag cttatcagag acaatgaatc gcttattcag
     6781 cttcatcatc ttgttgcgct cctttcatga actaagccac atattgccac tggcgcaagg
     6841 tgcgcgcaga gggaggacaa tcgcctcctc cccctgcacc cccgggctct
//
LOCUS       DAJTMK010000192         6865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTMK010000192 DAJTMK010000000
VERSION     DAJTMK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.196"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            4..420
                     /locus_tag="OO135_003574"
     CDS             4..420
                     /locus_tag="OO135_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5399272.1"
                     /translation="MPPFFTEPERNMAKKDENLKRLKELSELLGREPDMSGSAADIAQ
                     RVAELEEELGGVDGTDNQASVLTGQENNGEAVPPERMIQNTDELVRVVARTTLHIDAL
                     HATQDKPVKLVLPETVFRVPAGMATSLVKDDLVKMQ"
     gene            432..800
                     /locus_tag="OO135_003575"
     CDS             432..800
                     /locus_tag="OO135_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399273.1"
                     /translation="MADFDNLFDAALDLADRAIIRNMGITAVITSGQLKGHRISGVFD
                     DPESISLVAGGVRIEDSLPSLFVKTADILQLCRNDSLMIGRESFSVDRITPDDGGCSY
                     IRLRREGAGNVRAGGYYEGA"
     gene            787..1374
                     /locus_tag="OO135_003576"
     CDS             787..1374
                     /locus_tag="OO135_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399274.1"
                     /translation="MRVLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            1371..1595
                     /locus_tag="OO135_003577"
     CDS             1371..1595
                     /locus_tag="OO135_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399275.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVRLRSYCATTAC"
     gene            1774..2460
                     /locus_tag="OO135_003578"
     CDS             1774..2460
                     /locus_tag="OO135_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5399276.1"
                     /translation="MYTGKGDAFANPLSDTDWLRLAMVKDLQPGEMTADAEDDTYLDD
                     EDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDSGEVCAFRIKYPNGTVDVF
                     RGWMSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVGVTGLTVAPASVSVKAGAT
                     TTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKGVKQGSVSIVGMTSDGEFV
                     AVAAVTVSAP"
     gene            2519..2905
                     /locus_tag="OO135_003579"
     CDS             2519..2905
                     /locus_tag="OO135_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5399277.1"
                     /translation="MFLKQDTFNYEKQSVVLSELYGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLLSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2914..3242
                     /locus_tag="OO135_003580"
                     /pseudo
     CDS             2914..3242
                     /locus_tag="OO135_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            3214..6255
                     /locus_tag="OO135_003581"
     CDS             3214..6255
                     /locus_tag="OO135_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5399278.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAGEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     VWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAVLNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            6255..6584
                     /locus_tag="OO135_003582"
     CDS             6255..6584
                     /locus_tag="OO135_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399279.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6584..>6865
                     /locus_tag="OO135_003583"
     CDS             6584..>6865
                     /locus_tag="OO135_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5399280.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVAGMAED"
BASE COUNT         1666 a         1559 c         2218 g         1422 t
ORIGIN      
        1 agtatgcccc cgttttttac tgaaccggaa agaaatatgg caaagaaaga cgagaatctg
       61 aaacgtctga aagagctgtc agaattgctg gggcgtgagc ctgatatgtc gggaagcgcg
      121 gcagatatcg cacagcgtgt ggcagagctg gaagaggagc ttggtggtgt ggatggcact
      181 gacaaccagg caagtgtgct taccgggcag gaaaataacg gggaggcagt tccgccggag
      241 aggatgattc agaatacgga tgagctggtc agggtggtgg cacggacgac gctgcatatt
      301 gacgcgcttc acgccacaca ggataagcca gtgaaactgg ttctgccgga aacggtgttt
      361 cgcgtgcctg ccggcatggc caccagtctg gtaaaagatg acctggtaaa aatgcagtag
      421 caggggggat tgtggctgat ttcgataatc tgtttgatgc cgcgcttgat ctcgcagaca
      481 gggccattat tcgcaatatg ggcattactg cagtcattac atcaggccag ttaaaaggac
      541 accggatttc cggcgttttt gacgatcctg aaagtatttc tctggtagcc ggcggtgtgc
      601 gtattgaaga ctctttacca tccctgtttg tgaaaacggc agatatttta cagctgtgtc
      661 gcaatgattc gctgatgatt ggccgggagt ctttcagcgt ggatcgtatt actcctgatg
      721 acggcggatg tagttatatc cggttgcggc gtgaaggagc aggaaacgta agggcagggg
      781 gatattatga gggtgcttga gaatgccatc cgcaatctga acagtcttga tacccgtatg
      841 gtgccgcagg ccagcgcatg ggcagtaaac cgggttgctg caaaagttgt ctcggttgcc
      901 acccggcagg tcgccaggaa taccgttgcg ggagataacc aggtgaaggg gggccccctg
      961 aaactggtgc gtcagcgcgt ccgggtattt aaagccagtc cggcaggaaa aatgtatgcc
     1021 cgtatccgtg ttaaccgggg caatctgccc gccattaagc ttggcacaac atgccggcgt
     1081 gccggtgacg gactgaaggt ggggaaatac ttttttcgtg gtgcgtttat ccagcagctg
     1141 gcgaacggtc gctggcatgt tctcaggcgt cttcctgaag cgtgttttga aacaggaaag
     1201 gacagtaagg ggcggcccag aaaaaatcgt ctgcccgtgg aggttgtgaa aattccgctg
     1261 gccggaccgc tgacacaggc atttgaggat gcccgcgacc gcatcattgc cgcggaaatg
     1321 ccgaaacagc tggggtatgc cctgaaacag caactgagga tacatttggg atgaacaggc
     1381 acacacaaat ccgccaggca gtactggcaa gcctgaaggg cgaatgcggg gggaatgtcg
     1441 tgctttttga cgggcttccg gcatttattg atgcgcagga actgcccgcc gtggcggtgt
     1501 ggctgagtga tgcgcagtac accggaaaaa tgacggatga ggacgactgg caggctgtac
     1561 gcttacgttc atattgcgcc accactgcat gctgatgtac cacagcccgt cacgaatgag
     1621 ccagtcacgc tcttccttcc cggtggagta cggatgtgct gtcagccgtc acaacttacc
     1681 gatgtcttca gggtaagcgt caatacggtg ctccgttgac gcttaccacc aaatccactg
     1741 gcaaagacga aaggtgcggg aacgacgttc tggatgtaca ccggcaaggg cgatgcgttt
     1801 gcgaaccctt tgtcggacac tgactggctg cgtcttgcga tggtgaagga tctgcaacct
     1861 ggcgaaatga ccgctgatgc agaagatgac acttatctcg atgatgaaga tgcagactgg
     1921 aaaacgacaa cccaggggca gaaatccgtc ggtgatactt tggcgacgct ggcctggcgt
     1981 ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg attccggtga agtctgcgcg
     2041 tttcgtatca aataccccaa cggtacggtt gatgttttcc gtggctggat gagttcactg
     2101 ggtaaaacca ttacctcaaa agaggtgatg acccgtactg taaaaatcag cggtgtgggg
     2161 cgtccgtatc tggcagagga gggcgctgaa accgtgggtg ttaccggact gacggtggca
     2221 ccggcatctg tcagtgtcaa agcgggagca accaccacac tgacctttac agtaaaacct
     2281 gatggggcca gtgacaaagc gatcagtgtg cattcgtcag atcctcagac tgcttcggtg
     2341 accctgagcg ggcttgtggc cacggtgaaa ggcgtgaagc agggcagtgt cagcatcgtg
     2401 ggcatgacct ctgacggaga gtttgtggca gtggctgcgg tgaccgtcag cgcaccataa
     2461 caggacgata ctcatcattg ccccggttat ccggggcttt ttgcatccgg agaacatgat
     2521 gtttctgaaa caggatacgt ttaattatga aaaacagtcc gtggtgctca gtgagctgta
     2581 cgggctgcag agaattgaat atctgacgtt tgttcagcag cgaacggcaa agtttgatgc
     2641 acaggaggga gaactgccgg aggctgaacg acagattgct tttctgcgta tgggaatgga
     2701 tatcaatgcc tggctgcttt cccgctcact gtggaatgct gagcagtctc aggatgttga
     2761 gacgctttgc gcatccatta tgacaacatg gtcgtatgat gcgctgggcg cgggggcgga
     2821 gagggttctg tcgctgagcg gtatggggac cattgagaat gccggggatg atgatcatga
     2881 ggcgctgacg ccggaaaagt cctgacgcgg gaaatgcagt ttgtcatgcg gcttgcccgg
     2941 gagttccggc gggcagactg gcggcggatg ctgtcggaaa tgtcggccac tgagcttggt
     3001 gagggggcga ttatttccgg atgcagagct tcagtgatgt gtggatggat gcgcagtttg
     3061 cctcgctgaa ggcattgatc gtgagaatgg tgtccggcag cagtgatgct gcggtggctg
     3121 atttcagcct tttaccggaa gagaacggga taccggagcg aacggacgaa gaactgatgc
     3181 atcttgggga aggtatttcc ggaggtgtgc gttatggacc agatagccaa cctggtcatt
     3241 gatttgggga ttgatgcggc agagtttaaa aatgaaattc cccgtatcaa aaaccttctg
     3301 aatggtgcag ccggtgatgc agaacggtct tctgcccgta tgcagcgttt tatggagcgt
     3361 cagactcagg cggcccggca gacaatgcat gcggcttctt cggctgcaac agcagcatca
     3421 gcccatgcgc agacggtgga gaagaatgca cgggctcatg aacgcatggc ccgtgaggtg
     3481 gaacaaaccc gtctgcgcgt ggatgccctg aatcagaaaa tgcgcgagga acaggcgcag
     3541 gccagggcac tggcggaggc gcaggataaa gcggctgccg ccttttatcg ccagattgac
     3601 agtgtgaaac aggccggtgc ggggcttcag gaattacagc gtattcagca gcagatccga
     3661 caggccagaa acagtggcgg ggttggtcag caggattatc tggcgctgat ttcggagatc
     3721 acggcgaaaa cccgtgccct gacgcaggca gaggaacagg ccacccggca gaaagcggcg
     3781 tttatccgcc agcttaaaga gcaggcaacc cgccagaatc tgtcgtcttc tgagttgctt
     3841 cgcgccaggg cggctcagct gggggtaagc agtgctggag aagtgtatat ccgcaaaatg
     3901 gagcgggcag gaaaagccac acattcgctg gggctgaaaa gtgcggcagc ccggcgggag
     3961 ctgggggtgt taatcagtca gatggcgcgc ggcaattttg gtgcgctgag gggatccggg
     4021 ataacgctgg ctaaccgtgc cgtatggaca ggcgcactga tgtcgccgaa aggcatgatg
     4081 actggcggcg ttattggcgg acttgtcgcg gcggtcctgg gtctgggtaa agcctggcat
     4141 gacggccgga aggagggcga ggaatttaac cgtcagctgg cgctgacggg acattatgcc
     4201 ggtgtcactg tcgggcagtt gtggaaactc agccgggcca tatccgggaa tggtatcacg
     4261 caacatgcgg cagccggtgc gctggcacag gtggtgggca gtggggcatt tcatggaaac
     4321 gatattggta tggtggcgaa agctgccgca cagatggagc gatcggttgg ccagtcggtc
     4381 agcgatacca taaatcagtt taagcggctg aaggatgatc ctgtaaatgc cgcgaaggcg
     4441 ctggacaatg cgctgcattt tttgactgcc actcagcttg agcagatacg cgtccttggg
     4501 gagcaggggc ggtccagtga tgcggcccgg atcgccatgt ctgcgctggc agaggaaacc
     4561 ggtaaacgca catctgatat tgataataat ctcaatgcgc tgggtagtac gctgcaaacc
     4621 ttgtctgact ggtggaagca gttctgggat gcggccatga atattggtcg tgaagactcg
     4681 ctggatgcgc agattgatgc gttacaggaa aaaattcagc gcgcgaaaaa atatccgtgg
     4741 acaaacgcct ccacacaggt ggagtacgat cagcagcgtc ttaacgatct tcaggagaaa
     4801 aaacgccgga aggatttgca ggatgcaaaa gcgcaggcag aacggaatta ccaggagcaa
     4861 cagaaacgcc ggaatgctga aaatgccgtg ctgaaccgga tgaatgaaac ggaagctgca
     4921 cgacatcagc gggaaattgc gcgtattaat gccatgcagt acgccgacca ggctgtcagg
     4981 gatgcggcga tacagcgtga aaatgaacgt tacgaaaaag ccattaagaa aaatacacgg
     5041 gcaacccgta atgatgaggc cacccggtta ttgctgcagt acagtcagca gcaggcacag
     5101 gtggaagggc agattgctgc tgccagacag tcagcaggca ttgccactga aaggatgact
     5161 gaagcgcata aacagcttct ggccctgcag cagcgcatca gcgacctgga cgggaaaaag
     5221 ctgacggcag atgaaaagag tgtgctggcc cgtaagaatg agctgattca ggcgctgacg
     5281 ctgctggatg tgaaacagca ggagctgcag aaacagacag cgcttaacga cctgagaaaa
     5341 aaaacggttc agctgaccag ccagctggca gacaaagaac gtgcactgcg tgagcagcac
     5401 aatctggata ttgccactgc aggtatgggg gataagcagc ggcagcgcta ccaggcacag
     5461 ttgcgcatcc ggcaggaata ccggcaacag ttgcaacagc ttgagaatga cagtcgccag
     5521 aaaggcactt acgggacgga ggactaccgg agggctgagg aggtgctgaa ggggagcctg
     5581 aagcgacaac tgaatgaaaa caaacgctac tggcaggaac tggaagtggc gcagggcgac
     5641 tggaaaaacg gtgccatgcg ggcgtttcag aattttacgg cggatgcgga taatgcggcg
     5701 ggaacggcag aacagatgtt tacagtggca ttcagcagtg ccggtaatgc actggcgaca
     5761 ttctgtacca ccggtaagct gaattttaaa tccttcacct cttccctgtt gtcagatatg
     5821 gccagaatta tggcacagat ggccatgatg caggcggtaa agggcgtcgg ttctttattc
     5881 ggcttcacga ctaatgctga tggcggtgtt taccagtctg ctgatttgag tcgctacagt
     5941 ggcacggtgg ttaaccgtcc gacgtttttt gcttttgcaa aaggagcggg tgtgatgggg
     6001 gaagcgggac ctgaagccat tctgccactg cgtcgcggtg ctgatggtaa gctgggggtt
     6061 gtggcggata ctggtggttc aggcatggtg atgtttgccc cgcagtacaa catcgggatc
     6121 aataacgacg gcacgaacgg gcagataggt ccggcagcac tgaaggtggt ttatgacctc
     6181 gggaaaaaag cggccgcgga ctttatgcaa cagcaggccc gtgatggtgg ccggttaagt
     6241 ggagcatacc ggtaatggag acgtttcgct ggaaggtgcg cccggatatg aatgtggtat
     6301 cagagccaaa agtggtggca gtgaagctgg gtgatggtta tgaacagcgt cgcccggcgg
     6361 gactgaataa tctgctgtcg acttacagcg tgacgatacg tgttcgtaaa ggtgaacacc
     6421 cacctttaaa agcctttctg gaacggcacg gtggcgtccg cgcatttcag tggacgccac
     6481 cttatgactg gaaaccgatc agggtggttt gtcgtaaatg gtcggcaagc gtgggggcgt
     6541 tatgggtgac tgtaacggcc gattttgaac aggtggtgaa ctgatgcagg atatccggca
     6601 ggaaacactg aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat
     6661 cgacctgaca gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg
     6721 tgagccggtc acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt
     6781 tgaactgaat ggcaaaggca ccagtacgcg ccccacgctg acggtttcta acctgtacgg
     6841 tatggtcgcc gggatggcgg aagat
//
LOCUS       DAJTMK010000193         6859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTMK010000193 DAJTMK010000000
VERSION     DAJTMK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6859
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.197"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..394)
                     /locus_tag="OO135_003584"
                     /pseudo
     CDS             complement(1..394)
                     /locus_tag="OO135_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1084..2793)
                     /locus_tag="OO135_003585"
     CDS             complement(1084..2793)
                     /locus_tag="OO135_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5399281.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVRVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(3225..3944)
                     /locus_tag="OO135_003586"
     CDS             complement(3225..3944)
                     /locus_tag="OO135_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5399282.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(4069..4632)
                     /locus_tag="OO135_003587"
                     /pseudo
     CDS             complement(4069..4632)
                     /locus_tag="OO135_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4616..4874
                     /locus_tag="OO135_003588"
                     /pseudo
     CDS             4616..4874
                     /locus_tag="OO135_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4983..5561)
                     /locus_tag="OO135_003589"
     CDS             complement(4983..5561)
                     /locus_tag="OO135_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399283.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISEHTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5982..6219)
                     /locus_tag="OO135_003590"
                     /pseudo
     CDS             complement(5982..6219)
                     /locus_tag="OO135_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6406..6859)
                     /locus_tag="OO135_003591"
                     /pseudo
     CDS             complement(6406..6859)
                     /locus_tag="OO135_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1856 a         1569 c         1259 g         2175 t
ORIGIN      
        1 gcatgctgta cccggtcgta ataaccgctc ctggatacac caggtacccg acacatcact
       61 tcaacaggga attgttgtcg ccattgttcg actaacgcgt atttttcagc gactcctggg
      121 caaaatacgc tgttgctttt tttaatatat ctcgctcaag tcgtgcttca tttaaagcct
      181 tacgcagttg cataacttca gattccagct cagccactgt gcgggagcct ggtgtattga
      241 gcccttttct tgcggcggta acccattgtc ctaaagtgcc ttcaggaaca gataatcggg
      301 aagcgccttc actgatcgaa agctgatttt caggaaccgt tttgacggct tcggttttaa
      361 actccgtaga gtaacgttgg gtttttctgc tcattattgg ctccttctga tgccattcta
      421 tttcaggaag gagtgtccgt taaactcagg ctacctcaga taagcttgat acaactggtc
      481 tggtatatac agtttctttg agtgcggtta tgtctacaga ggtaacggta aaagtaactc
      541 cttgtcggga ccactccgac cgcgcacaga ggcattttca tcgctgacag gccgcattgc
      601 ctcgcagggc tggaggagga tcggtgatat ccggacgacc gtaccggctt acgccgggct
      661 tcggcggttt tactcctctg ccatgtgcaa caacggagtg ccgccttaca tgccgctggc
      721 gcggcatatc ctggtaacat ttttgttgat accaccttcc ggccttatcc tgaccagtgg
      781 gctttcctgg catcgatgat accgatgaac gcgcatgata ttgagccgac gatattacgg
      841 gcaactggaa atactcaccc tctggatgtg acctttatcc atgaagaaga tttggccata
      901 ccatggaaac ctgaacaatc atcagtttac gcccatgtaa acccctacgg gatatttgaa
      961 ctcgatatgg aaacaaggtt gccaatcgag gtggttgcct gaaagttggc agttacccgg
     1021 tcactacacc ttgttggagt gaacatatca aaaacaggta attatacaat ttaaattaca
     1081 gtctcataaa aaatgattaa atctatttat tatttcaata aacgtttttt tcatcttctt
     1141 tgaaaattgt tctgctatag tatccggttc cagattattc tcttttatta ttctacaata
     1201 ctctaatatt gtttctttgt ctctatttac tacagataca aatacaccag gaataccatc
     1261 tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat agttttttag
     1321 aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca taaaaagtcc
     1381 tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct ggtgaggggg
     1441 gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat tttgaagtgc
     1501 cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac taagcatttt
     1561 cttattaagc agacttccga acatacgaat actatctgca tgtccattct gcaatgcaaa
     1621 aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc tcttatcgga
     1681 agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta catgtccgtt
     1741 cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa gcaatgtaag
     1801 tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt gaataatgtc
     1861 tgaacatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc ttctcgtggc
     1921 cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt ccatcagtat
     1981 aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg aattgatcaa
     2041 ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat
     2101 tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa aaaaatcctt
     2161 agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac gtttatgagt
     2221 aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt gtacgcgctt
     2281 tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg ttctgacaaa
     2341 gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc actttttatt
     2401 ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt ccaaacatac
     2461 attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc catttgtttc
     2521 cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc ttccatatat
     2581 atcttttgcc actccattta tatttaccct cacatttctc ttttctgaaa aataaggtgc
     2641 tgttttattc ttaaactgaa ttagttcact ggagtttgag accctcataa tagaacttgt
     2701 tattgttcta ttatctctgt taacagacat atctgttgat aacgaatttt ttgttgaaaa
     2761 tatactagat gaaaatgttt tatttaacgg catagaagac aaccatagag atgttataat
     2821 actattagat tgcagttact aatagtaaaa gcctccttac aaggaggtag cttattcaga
     2881 atgctcctat aagatagcga gctcgtcaac gatacagatg atctgattat attgtgcaaa
     2941 aggagaacag gaaaataggc actggaaaag atgaaggcct gatgggagca ttaactaatg
     3001 caaaatccga caggttaata tcccgaaaag caatcaaacg actcaaacag gagatgcacg
     3061 ctgaagtggt ggacaactgt tcggatttca gatgagtagc tcagcagttt atgagccgaa
     3121 tgctctgtca cggtcaggaa tcaggctgca tacgctgtgc caaatctaat cgtagttatt
     3181 ctaaccagtt ttcaagctac aaggtggtgt agctggcatc ttctctactc tatcatcaaa
     3241 cgataaaatg gttcctctcg taacatttgc tcttgccttt cacttccatc tcgatcgctt
     3301 cgtagttcat tacgatagct gacatatttc cagtcttcag gtctaacatc tgacgccgga
     3361 tactccccgg gagcgatttt gcttcttaat agattagact cgagacacat tataaattgt
     3421 cgtattttat gaagtaataa agcactatat tgcgagcatt cctgatctga ttttacatat
     3481 agcgtaaact gagctcctat ccccacacga gattgctgag aaactcgatt catatcagtt
     3541 atcttccatt tatctatagg actgtcctct gaaaataata aaccagacaa aatttggaat
     3601 gccagaggaa cttgctccct agctatacta atatgaaact tgtccccaac aaaatccccc
     3661 ttactctgag gattttcacg acatgcatga ataaacacat ctccactatg gttcacaatc
     3721 tggaatcctt ggtacatgac agaaaaaaat tgacatatat cagagtaagt tggcacgttc
     3781 gatgctactt tgaaatagta cgctgggtat tgccgtattt ggttgtacaa tatattgaag
     3841 ttatttttca atctttcatt cgcagaaagc atctgcggta tttcagacct cacaacattc
     3901 aaagaatcta aatttagttt tagacagggt ttttttatgg gcatagaaaa cgtcctctat
     3961 aaagtagata tattatctta tattgattgt ttatcatctt ttataaaacg atacattata
     4021 tgtaatgtat gtaagcgtaa gggtcttctc cgatcatggt gggaaggctt agaacgccat
     4081 attcagtttc ccatagtgga acatcactcc cagcttaaag cgctcccggt tgcggtagcc
     4141 cttggctttt attctcagca gcctgatctt gctgttcagt gcttccgcat ttccgtttga
     4201 tacgccatgg cgcatggcgt tcaggatacc gtacaacctt ttccctatcg ttttagccac
     4261 attcttcatc atggggacgt cactgttggc cgccagcgac agccatctct gccagtcgtt
     4321 gcgtctttcc tcgctccatg gtctgctcca gatatccttt gccagttctt tcagcgccca
     4381 gcacaggctc gtcagcttca tctgcgcacg cagccatatc agcttttcct gcctggattc
     4441 ggtcatccat ttatcgctgt gctgccacag gaagcgagtt ccctttgcct gacgacgact
     4501 ttcaacagga agatgcggat gttcattctg gcgggtttta tccaccacct cacccggttg
     4561 tttcgccaca tgaaagcgat cgaaggctat tttctcaacc gcattgggta agtggatgcg
     4621 cgctgctctt agtttgctga gccagtttag cattgccttc tacatgaccg atgcctgagc
     4681 cggtataccg aactttattg tcctcaacca gtcatgttat aagcaagaaa tgcactcagc
     4741 ggatagagcg acataatctg aacctgcgga cacatcttaa gcggcttacc cgcaaaacca
     4801 tatgcttctc aaagtctgat gatatgcact acaagatcat cggctggtat ctgacaatca
     4861 accaccacca ctaaatctgc gtcacgacct gcaactgagt ctggttgaac aggctgaggc
     4921 tctgctgaag caacgcgaac ctctcgactc ttgttggatt ttaccaaata gtttttctaa
     4981 aattaaacac atttatataa gttctttgct gaacaaaacg cagcccgcca tcctttttca
     5041 ttcataaatt cagcaatggc agataaaact ttttcatcaa gcatataaaa aataatgcaa
     5101 gtagactcaa tatgcctcat acaaataagg ccgcacatat ccgtaatata tttgtcaatg
     5161 ctgctataat ttgcatggta atatgccatg ctattatctg atgattttac atatgcagta
     5221 tgacatattt ttccttcacg attaacaaaa ctgacaaaac cactttcacc aggagtaata
     5281 tataaagatg aatttaacgt gtgctcacta atattcatta attgttgaaa ttcaatggaa
     5341 tagttttcag ttaatgtatc tattatcttg gctttgtctt tctttgttat ctcattgtct
     5401 ttttcggcaa gaaaaattgc cactttatga catgcattca gctcgttaat tttacctgat
     5461 aaaactgctt ttcttaccac atcgttgaac tcatcgatag tgtgtttttt attatatata
     5521 tcatgaaaaa tatagcctaa agacttaaac gagactttca ttttatattc tccttaaata
     5581 ttaacaggat atatatatca cattttcctc tatattttca gaaaccagct atcattttcc
     5641 ccattagtgg tacgtcgctt cagatatttc cgcagcaagc gataaccctg cgcactatga
     5701 ggatgccatt cattataatg tttgaacacc ttcgcgagac tactttactg ctgttaacac
     5761 gtcaggttcg aacataacac tgatttaatc atatttcatc gttttgacga agttttctgc
     5821 cattccattt tgcaccgccg tgtgtttggg ggcaaggccg accatctgtg tttcattcgc
     5881 cagatataag tgatgagatg gcattctccc tccttcacgg cagaagcatt ttgaccgttt
     5941 aggggatgcg gacgattttc atgcctgcta ggatactttc gttactgatg ctgtccgttt
     6001 tccaccattt catcatcact gattggcgtt gtgtttttgg tcagcactcc agctttacgt
     6061 ttctctttta accggtaact ctctcctttg atattcagcg tgtagcagcc gatctaggat
     6121 cgctgttgcc agcacgtgat ctccgaacat ttctccccag tctgcgaccc ctttgtttga
     6181 cgtcagtatg atgctcgctt tttcatatcg acagttcatc gataggaatt taaatcccca
     6241 aaagattaaa aaagcaccga agaatgcgcg tttattcaac acaacctgat catcaagtaa
     6301 tcggtataga cacttaatat tgcttacatt gattgattca atgtatctct gatggttttt
     6361 tgctccgttc ccctcaacat ggacgcaact tcccctctgc gactctcagc cgcaccaccg
     6421 tattcgagcc agcggctcat gcatcataac ctgctctgcc agacggtaac ccctcttcag
     6481 ccccgtaaac cgcatctgac ctccgcacag ctcgcacttc agcgggtcaa ccttcagtca
     6541 ctgctgatac atccctctcc ttgtgatttg cctcgccgtt tttctcactg tctccgttat
     6601 ggcgtacacc acatcttcca gtaatcgccg cttcaccgga ctcaggaccc cgtatcacca
     6661 tccggaaccc cttatccgcc acatgccagg agaacctttc cataaactcg tcgccactca
     6721 tcacaaggtt ttcttctcgt tttgtccggt ggctgttgta acgcaggcca atttcatcct
     6781 gaccggcata atgttccagg cggctcatct gcaccggctg cttctccagc cagttaagcc
     6841 atcggtttcc ctgctgaaa
//
LOCUS       DAJTMK010000194         6837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTMK010000194 DAJTMK010000000
VERSION     DAJTMK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.198"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..768)
                     /gene="uxuR"
                     /locus_tag="OO135_003592"
     CDS             complement(<1..768)
                     /gene="uxuR"
                     /locus_tag="OO135_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5399284.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVD"
     gene            complement(983..2443)
                     /locus_tag="OO135_003593"
     CDS             complement(983..2443)
                     /locus_tag="OO135_003593"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5399285.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2524..3708)
                     /gene="uxuA"
                     /locus_tag="OO135_003594"
     CDS             complement(2524..3708)
                     /gene="uxuA"
                     /locus_tag="OO135_003594"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5399286.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4048..4252
                     /locus_tag="OO135_003595"
                     /pseudo
     CDS             4048..4252
                     /locus_tag="OO135_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4495..5397)
                     /gene="fimH"
                     /locus_tag="OO135_003596"
                     /pseudo
     CDS             complement(4495..5397)
                     /gene="fimH"
                     /locus_tag="OO135_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(5417..5920)
                     /gene="fimG"
                     /locus_tag="OO135_003597"
     CDS             complement(5417..5920)
                     /gene="fimG"
                     /locus_tag="OO135_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5399287.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(5933..6463)
                     /gene="fimF"
                     /locus_tag="OO135_003598"
     CDS             complement(5933..6463)
                     /gene="fimF"
                     /locus_tag="OO135_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5399288.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6473..>6837)
                     /locus_tag="OO135_003599"
     CDS             complement(6473..>6837)
                     /locus_tag="OO135_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5399289.1"
                     /translation="ALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTL
                     THNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCIANYQ
                     LPPESQQQLLTQLSAECR"
BASE COUNT         1592 a         1852 c         1732 g         1661 t
ORIGIN      
        1 gtcgacttta tccagcggcc atgaatcaaa cagatagccg tcaaaataaa tatcgtcaac
       61 gttcgagaat tccagcagac gttgcttaac gttttccaga tgctgccaca ttgccagctt
      121 cgccgctcgg gcatcttttt tgattaacgc ggcaaggatc tgtttgtgat cgcccaacca
      181 ctctttgcga tacaggctgt catccagatg gctgtgcaac tggatccaca ttggattgtt
      241 ttcccgccac tgccaggact gacggaataa ctccaccagc atgctgttat gcgttgcttc
      301 agcaatggcg agatggaact gcatgtcacc gctttcgctg ctacccggcg cgctggaagc
      361 cagttcacgc tcttccagtt gcaatgcctg acgcattttg acgatatctt cgcgggtagc
      421 ctgcagggcg gcaaattccg cgatgttgct ctcaagaagc tgtcgcgcct gcaacaattc
      481 aaaggggccg gcatcattgc agacgttggc atccggactg tctgtgttgt ggctgcctga
      541 gctatcaaga acatagatac cggcaccccg gcgcacttcc accagccctt tgatctccag
      601 catgatcagc gcttcacgta ccaccgtccg cgtgacatca agcatttctg caatttcacg
      661 ctccggcggc agccgttcgc caggattgta cggcgtcttt atgatcagat cgcggatcat
      721 cgccccgact tcctggtaag gtctttgcgc agaggtggca gatttcatgg tgttgtgtcc
      781 gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg aacgccttat ccggcctaca
      841 acgggcaatt ttacggcccc cgttaatctc aaactatacc attagcactg gaagttggca
      901 cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat atcagtttga ttgaccagta
      961 agtctgtagg gtaatcagtt agttacagcg cagccacaca ctcgcgcgca ccgcgttcgc
     1021 acagctgctg atatgccgcc gtcaccgcgc caacaaagtc ggcattctgc ggcagatcat
     1081 cggcaaaaat accgctcagg cccagcagcg ctttcacgcg gtctgcgccc tgatactgcg
     1141 cgttaatctt ctggaactcg gccagcatcg ggtcgaccac gtcaatggca ttaccctgct
     1201 catccacgcc ctgggtgtaa cgcatccagc cagccacgcc cagcgccagg tgacgccagc
     1261 tgccgccgtt ttgcaggtgc agacgcaccg ggtccagcag acgctgcggt aacttctggc
     1321 tgccgtccat cgcaatctgc caggtacggt gacgcagaga cgggttgctg aaacgctcga
     1381 tcagcagcgt cgcataggcc ttcaggtccg taccctccgg catagacagc gttggcgctt
     1441 gttcctgcat catcagggca aaggccgctt tgcgataatc cgggttagtc atggtgtcgg
     1501 caatagtttc atagccgccg aggtaaccga ggtatgccag gaaagagtgg ctgccgttca
     1561 gcatacgcag cttcatcatt tcgaacggca caacgtctgc aacgaactgt gcgcccactt
     1621 tatcccagtc cgggcgacca ttaacgaagt tatcttcaat cacccactga cggaacggtt
     1681 cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc agcaatttcc tgtaaggttt
     1741 ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca cgggaaggtg acgttctctt
     1801 caatccatgc cgccagctgc gggtcacgtg cctgagccag ccccagtacc gcgaccttcg
     1861 ccacatgacc gttttcacgc acgttatcgc aggacatcac cgtaaatgct ttcagccctt
     1921 tttcacgacg cagacgcaag gcttcgacga tgtaaccaat cgcggacttc ggcgcagtcg
     1981 ggttttctag atcgtgcttg atcagcgggt tattgagatc cagttgaccg cttgccgcat
     2041 cagcgcagta gcctttttcc gtgaccgtta gagagacaat cgccgtttgc ggacgcgcca
     2101 tcgcgttgag aataccttcg cagccgtcga tttccggatg cagcgcttct ttcatcgaac
     2161 cgataatttt cagctcggtg ctttctgcgc ctttttccgc tacggtgtac agcagttgct
     2221 gttttttcag gttttcgatc agcacgcggt cgttgcctgg catcaggtta acttcgcaga
     2281 tgccccagtc gctgtcggtg ctttccagca gatgatgggt atacagcgcc tgatgtgcgc
     2341 ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc cagacgagaa tgatcccatg
     2401 acgggcgggc aaccggcaga ttgctgtcaa caatagtagt catcgtaaac tccagaatcc
     2461 gtcttcagag agtgacggca aggaagaagg aaccggcacg gcgcgcagcc atgccggtgg
     2521 atattaacgg ctaaagaaag cgcgctggat cgccagttcg acaccgcgaa cttcggccag
     2581 gcctttcaga cgaccaattg cggagtaacc tgggttggtt ttcttcttca ggtcgtccag
     2641 catctgatga ccgtggtccg gacgcatcgg gatcaggtct tctttgcctt ccgctttacg
     2701 acggtgttct tcttcaacaa tcgctttcac cacttcgtac atatcaacgt caccgttcag
     2761 gtgcgccgct tcatggaagg ttttcgggtt atcttcacgc atggtggagc gcagatgggt
     2821 gaagtaaata cgcggaccga actgcttgat catatcaacc agatcgttgt cagcacgcac
     2881 gccgtaggaa ccggtgcaca tggtaaaacc gttcgccatg ctgtttacgg tatcaaccat
     2941 ccactgcata tcttcaatgg tggaaacaat gcgcggcagg ccgaggatcg ggcgcggcgg
     3001 atcgtccggg tgaaccgcca tacgcacgcc aacttcttca gcaactggaa taatcgcttt
     3061 caggaagacg gcaaagtttt cgcgcagctt cgctttatcg atatctttgt acagctccag
     3121 gtgcttacgg aactggtcga gggtataccc ttcttccgcg cccggcagac cggcaatgat
     3181 gttacgcgtc agacgcgctt tgtcttcatc gctcatagtg gcgaagcgtt cagctgcctg
     3241 agcaatttct tcttcggtgt aatccgcttc cgcgcctgga cgtttcagga tatgcatttc
     3301 gaatgcagcg aattcgatct ggtcgaagcg cagagctttg gagccgtctg gcagcacgta
     3361 ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag ttgtagcaca cggtgcgaat
     3421 gccgcactgc gccaggttgc gcagagtctg ctgatagtta gcgatccact gctcatagtt
     3481 gccagtgtgg gttttgatat cttcgtgaat tggtacgctt tctacgacag accacaccag
     3541 gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct tctacggacc atacttcgcc
     3601 gttcgggata tggtgcagcg cggtaaccac gccagttgcg cccgcctgac ggacatcagc
     3661 taaagaaacc agatcgtttg ggccgtacca gcgccaggtc tgttccatga tttttcctct
     3721 ttcacatagt gggttccaca gcaaagagac gcggaaccct ggagttgttt aggtatccgg
     3781 atgaacaacc agaccgcagc cgtggcgttg catcggatga gatggcgtat aagttctacc
     3841 ttttggtaag atatgtcaac cagaattctg aaattggtta accaaatcac aagaatttca
     3901 ctctcatggt tgttgctgca aattgcagca aaaggtaatc gatggttatt gatcagtgga
     3961 tgtgacattc atcgcaacaa tggttgacca atttacataa catgtcgccc aaaataacaa
     4021 cggttcaacc aggtaaggta aaacgttatg catgtgctta acattctctg ggtagtattc
     4081 ggcattggtc tgatgctggt actgacaacg accgcaggtt cagacacatt cactgcatta
     4141 gcaatgcctc ttagattatt ttgacctgtc agtaaaagac acgttgaaaa cctggggtct
     4201 gctggagctg gtcaactccg tggttgggct gattattagc aaggtagcgt aaaagaacac
     4261 cagagcctgc caatggcagg ctctgactga tgacaaagtc taaaaatgtg cccggacagt
     4321 cccctcgccc ctccggggag agggttaggg tgaggggaac agaccagcaa agtgcgcttt
     4381 gtttatgcca gatgcgacgc tgacgcgtct tatctggcct acaaagggct aacgtgcagg
     4441 tttttagctt caggtaatat tgcgtaactg cattagcaat gccctgtgat ttctttattg
     4501 ataaacaaaa gtcacgccaa taatcgattg cacattccct gcagtcacct gccctccggt
     4561 acgtgcgtaa tttgccgtta atcccagact taccgccgaa gttcctactg ctcctaacga
     4621 taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg
     4681 cgctggtgaa aacgacgcgg tattagtgaa aatcgagttg cccgcatctg cagttgtgcc
     4741 ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag gaattggcac
     4801 tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat cgcagccgcc
     4861 agtaggcacc accacatcat tattggcgta aatattccac acaaactgga aatcatcgct
     4921 gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag ctttaatcgc
     4981 caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg gcttatccgt
     5041 tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat agctactgcc
     5101 actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg agcctcgttg
     5161 cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga tttgcgtcga
     5221 aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta cataaacatt
     5281 agcgctgcca ccgccaatag ggatagcagt accattggcg tttttacagg cgaatgacta
     5341 ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc gtttcattac
     5401 aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc tgaatggttc
     5461 cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag tgcgctgatt
     5521 gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg ttgccactgt
     5581 catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa tatccggtac
     5641 tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga caattagtca
     5701 actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga ctgaaagaat
     5761 aaagatcgcc gagatcaacc gtggcattgg tggtggaaac tgtgcacggt ttggcgacga
     5821 ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat tgtcgcactt gcgagcgcca
     5881 atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc agttactgat
     5941 attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc acctgtgtcg
     6001 ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg gtcgtccacg
     6061 aaatcgcgga cgatggagca ttaaggggta tctgattttg ctgctcattc agaagctgta
     6121 ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg gcattgtggc
     6181 tatctgcaac gccggtaaac ccaaccttta cggcagaaac ggcgttacca cagggtgaca
     6241 gcaaaatacg aaatggaacg acaggagtcg ttgcgccaat gttgttaaat tgcttcgccg
     6301 cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta cagccgttat
     6361 ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga cttaccgcca
     6421 gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc ccttaacgac
     6481 attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt tgataattgg
     6541 cgatacagtg agcattttcc tcttctcccc atttcacctg aacttttccc gctaaaggca
     6601 ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg ctactctctg
     6661 atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc atgagcagtt
     6721 ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg ggaacaacgt
     6781 tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc agcgcca
//
LOCUS       DAJTMK010000195         6808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTMK010000195 DAJTMK010000000
VERSION     DAJTMK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.199"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..1474
                     /gene="wzxC"
                     /locus_tag="OO135_003600"
                     /pseudo
     CDS             1..1474
                     /gene="wzxC"
                     /locus_tag="OO135_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
     gene            1846..3126
                     /gene="wcaK"
                     /locus_tag="OO135_003601"
     CDS             1846..3126
                     /gene="wcaK"
                     /locus_tag="OO135_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5399290.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNQHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASDAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGIQMVQSVLERIGEVK"
     gene            3123..4343
                     /gene="wcaL"
                     /locus_tag="OO135_003602"
     CDS             3123..4343
                     /gene="wcaL"
                     /locus_tag="OO135_003602"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5399291.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     TLAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            4354..5748
                     /gene="wcaM"
                     /locus_tag="OO135_003603"
     CDS             4354..5748
                     /gene="wcaM"
                     /locus_tag="OO135_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5399292.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVLGSDCTINGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFAIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIRV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            5906..>6808
                     /locus_tag="OO135_003604"
     CDS             5906..>6808
                     /locus_tag="OO135_003604"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCR5399293.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFV"
BASE COUNT         1668 a         1702 c         1799 g         1639 t
ORIGIN      
        1 gcttacgtga aaaaaccatc agcggcgcga agtggtcggc gattgccacg gtgatcatca
       61 tcggcctcgg gctggtgcag atgaccgtgc tggcgcagat tatcgacaac caccagttcg
      121 gcctgctgac tgtgtcgctg gtgattatcg cgctggcaga tacgctttct gacttcggta
      181 tcgctaactc gattattcag cgaaaagaaa tcagtcacct tgaactcacc acgttgtact
      241 ggctgaacgt cgggctgggg atcgtggtgt gcgtggcggt gtttttgttg agtgatctca
      301 tcggcgacgt gctgaataac ccggacctgg caccgttgat taaaacattg tcgctggcgt
      361 ttgtggtgat cccccacggg caacagttcc gcgcgttgat gcagaaggag ctggagttca
      421 acaaaatcgg catgatcgaa accagcgcgg tactggcggg cttcactttt acggtggtta
      481 gcgcccattt ctggccgctg gcgatgaccg cgatcctcgg ttatctggtc aatagtgcgg
      541 tgagaacgct gctgtttggc tactttggcc gcaaaattta tcgccccggt ctgcatttct
      601 cgctggcgtc ggtggcaccg aacttacgct ttggtgcctg gctgacggcg gacagcatca
      661 tcaactatct caataccaac ctttcaacgc tcgtgctggc gcgtattctc ggcgcgggcg
      721 tggcaggggg atacaacctg gcgtacaacg tggccgttgt gccaccgatg aagctgaacc
      781 ccatcatcac ccgcgtgttg tttccggcgt ttgccaaaat tcaggacgac accgaaaagc
      841 tgcgcgtgaa cttctacaag ctgctgtcgg tggtggggat tatcaacttt ccggcgctgc
      901 tcgggctgat ggtggtgtca aataactttg taccactggt atttggcgaa aagtggaaca
      961 gcattattcc ggtgctgcaa ttgctatgtg tggtgggtct gctgcgctcc gtaggtaacc
     1021 cgattggttc gctgctgatg gcgaaagcgc gggtcgatat cagctttaaa ttcaacgtat
     1081 tcaaaacatt tctgtttatt ccggcgattg ttattggtgg gcagatggcg ggcgcaatcg
     1141 gcgtcacgct cggtttcctg ctggtgcaga ttatcaacac cattctgagt tacttcgtga
     1201 tgattaaacc ggttcttggt tccagttatc gccagtacat cctgagttta tggctgccgt
     1261 tttatctctc gctgccgacg ctggtggtca gttatgcgct gggcattgtg ctgaaagggc
     1321 aactgggctg gggatgctgc tggcggtgca aatagccgcg ggcgtgctgg cgtttgtggt
     1381 gatgattgtg ctgtcgcgcc atccgctggt ggtggaagtg aagcgtcagt tttgtcgcag
     1441 cgaaaaaatg aaaatgcttt tatgggcggg gtgagttgct gttttcggtt ccgacaacgg
     1501 ttgccggatg cggcgtaaaa cgccttatcc atcctacggt tctgtgctcg ggacctgtag
     1561 gcctgataag acgcgtcagc gtcgcatcag gcactgaatg ccggatgcgg cgtgaacgcc
     1621 ttatccatcc tacggttctg tgctcgggac ctgtaggcct gataagacgc gtcagcgtcg
     1681 catcaggcac tgaatgccgg atgcggcgtg aacgccttat ccggcctacg gttctgtgct
     1741 cgggtcttgt aggcctgata agacgcgcca gcgtcgtatc aggcaaaaaa cggcaacgaa
     1801 cacaacaaac acagcctcac ctggcaccaa atttatgagg aaaaaatgaa attacttatt
     1861 ctgggcaacc acacttgcgg caatcgtggc gacagcgcca tcttgcgcgg cttacttgat
     1921 gccatcaaca tcctcaatca acataccgaa gtggacgtga tgagccgcta tccggtcagt
     1981 tcttcctggc tgctcaaccg cccggtaatg ggcgatccgc tgttcctgca aatgaaacaa
     2041 cacaacagcg cggcgggcgt tgtcgggcgc gttaaaaaag tccttcgtcg ccgctatcaa
     2101 caccaggtat tgctctcacg cgtcactgac actggcaagc tgcgtaatat cgccatcgcc
     2161 cagggattca ccgacttcgt gcgcctgctg tcaggttacg acgccattat tcaggtcggc
     2221 ggatcgtttt ttgtcgatct ctacggcgtg ccgcagtttg aacatgcgct ttgcacgttt
     2281 atggcgaaaa agccgctgtt tatgattggt cacagcgtcg gtcccttcca ggatgagcaa
     2341 tttaaccaac tggcgaacta cgtttttggt cactgtgacg cgctgatcct gcgcgaatcg
     2401 gtcagccttg atttgatgaa acgtagcaat attactaccg caaaagtgga acatggcgtt
     2461 gataccgcgt ggctggtcga tcaccacacc gaagatttca ccgccagcga tgccgtccaa
     2521 cactggctgg acgttgccgc acaacagaaa acggtggcaa ttaccttgcg cgaactggca
     2581 ccgttcgaca aacgtctcgg caccactcaa caagcgtatg aaaaagcctt tgccggggtg
     2641 gtcaatcgca ttctcgacga aggctatcag gttattgcgc tctccacctg tacgggcatc
     2701 gacagctata acaaagacga ccgcatggtg gcgctcaatc ttcgccagca catcagcgat
     2761 cctgcccgtt accacgtagt gatggatgaa cttaacgatc tggaaatggg caaaattctg
     2821 ggggcctgtg aactcaccgt cggtacgcgc ctgcactccg ccattatctc aatgaacttt
     2881 gccactccgg cgattgccat caactacgaa cataaatccg ccgggattat gcagcagctg
     2941 ggactgccag agatggcgat tgatatccgt catttactgg acggcagcct gcaagcgatg
     3001 gttgcggata ccttaggcca gcttccggcg ctgaacgcac gacttagcga agccgtcagt
     3061 cgtgagcgtc agacaggaat acagatggtg caatctgtgc ttgaacgcat cggggaggtg
     3121 aaatgaaggt cggcttcttt ttactgaaat ttccgctgtc gtcggaaacc ttcgtcctca
     3181 atcaaattac cgcgtttatt gatatgggat ttgaggtgga gattgtcgcg ctgcaaaaag
     3241 gcgacacaca aaacaccctc gcggcatgga cgaaatacaa ccttgccgcc agaacccgct
     3301 ggttacagga cgaacctacg ggcaaagtgg cgaagctgcg ccaccgcgcc agccagacgt
     3361 tacgcggcat tcatcgtaaa aatacctggc atgcgctcaa cctcaaacgc tatggtgccg
     3421 agtcgcggaa cctgattttg tctgccattt gcggccaggt cgcaacaccg tttcgcgccg
     3481 atgtgttcat cgctcatttt ggcccggcgg gggtaaccgc agcaaaacta cgcgaactgg
     3541 gtgtcattcg cggcaaaatt gccactattt tccacggcat tgatatttcc agtcgggaag
     3601 tgctcaacca ttacactccc gaatatcagc aactgtttcg ccgtggcgat ctgatgttac
     3661 cgataagcga tttgtgggcc ggaaggctgc aaaaaatggg ctgcccgagg gaaaaaattg
     3721 ccgtatcgcg tatgggtgtg gacatgacgc gctttagccc gcgtccggtg aaagcgcctg
     3781 caacgccgct ggaaatcatt tccgtcgcac gcttaaccga gaaaaaaggc ctgcatgtgg
     3841 cgatcgaagc ctgccgtcag ttgaaagagc agggcatggc attccgctat cgcattctcg
     3901 gcattggccc gtgggaacga cgcctgcgca cgctcatcga acaatatcaa ctggaagatg
     3961 tggtagagat gcctggcttt aaaccgagcc atgaagtgaa agcgatgctc gacgacgcgg
     4021 atgtcttcct gttgccatcg gttacaggtg cggatggcga tatggaaggc attccggtgg
     4081 cgctgatgga ggcgatggcg gtcggcattc cggtggtttc tactctgcat agcggaatac
     4141 cggaactggt ggaggctgac aaatccggct ggctggtgcc tgagaacgat gctcgcgcac
     4201 tggcgcaacg actggcggcg tttagccaac tggacaccga cgaattggct ccggtcgtca
     4261 aacgcgcgcg cgaaaaagtt gaacacgatt ttaaccagca ggtgatcaat cgagaactcg
     4321 ccagcttgct gcaggcttta tagaggagaa cgtatgccat ttaaaaaact ctcccgacgc
     4381 accttcctga cggcaagctc ggcgcttgcc ttcctccata cccctttcgc tcgcgcactt
     4441 cccgcccgac aaagcgttaa cattaacgac tacaaccctc acgactggat cgcctcattt
     4501 aaacaagcct tcagcgaagg gcaaacggtc gtcgtgcctg ctggattcgt ttgtgacaat
     4561 atcaacaccg gcatcttcat tcctcctggc aaaacgttac acatccttgg aagcctgcgc
     4621 ggcaacggca gagggcgatt tgttttacag gacggcagcc aggtgacagg ggaggaggga
     4681 ggcagtatgc ataacatcac cctggatgtg ctaggctctg actgcaccat caacgggctg
     4741 gcgatgagcg gctttggtcc ggtaacgcag atttatatcg gcggcaaaaa caaacgggtc
     4801 atgcgcaacc tgaccatcga taacctcact gtcagccacg ctaattacgc catcttacgc
     4861 cagggatttc ataaccagat tatcggtgcc aacatcacca attgtaagtt cagcgactta
     4921 cagggcgacg ccattgaatg gaacgtggca attaacgaca gtgatatttt gatctccgac
     4981 catgtcatcg agcgcatcaa ctgtactaac ggaaaaatca actggggcat tggcataggc
     5041 cttgcgggga gtacttatga taataactac ccggaagacc aagcagtgaa aaactttgtc
     5101 gtggcaaata tcacgggatc ggattgtcgg cagttgatcc atgttgaaaa tggcaaacat
     5161 tttgttatcc gtaatatcaa agcccgcaat atcacgccgg atttcagtaa gaaagcaggc
     5221 attgataacg cgacagttgc tatttacggt tgtgacaatt tcgcgattga taatattgaa
     5281 atgattaata gcgccgggat gttaatcggc tatggggtaa ttaaaggcaa atatctctcg
     5341 ataccgcaaa atttccgagt gaataatatt caactggata acacccatct tgcttataaa
     5401 ttgcgcggca tccaaatctc cgccgggaat gctgtctcct ttgtggcact gactaacatt
     5461 gagatgaagc gtgcctcgct tgagctacac aataaaccgc aacatctttt tatgcgaaat
     5521 atcagagtga tgcaggaatc ctcagttgga ccagcattga gcatgaactt tgacatgcgc
     5581 aaagacgttc gcggcgtgtt tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt
     5641 catgcggtga atgaaagagg gcaaagttcc gtcgatatcg acaggattaa tcaccatatt
     5701 gttaatgtgg aaaagattaa ctttagattg ccggaacgga gggagtagat ttgcgaccat
     5761 tcataaactt actggatata atgttctaaa agattataat cctcacacta actgttaaag
     5821 ctggggctgg gcagcattga ctactcaggg aaggtactct tctttatctt gtgtttctta
     5881 cgtaacaacc agactaaagt gatttatgaa cgataacgtt ttgctcatag gagcttccgg
     5941 attcgtagga acccgactac ttgaaacggc aattgctgac tttaatatca agaatctgga
     6001 caaacagcag agccactttt atccagaaat cacgcagatt ggtgatgttc gtgatcaaca
     6061 ggcactcgac caggcgttag ccggttttga cactgttgta ctactggcag cggaacaccg
     6121 cgatgacgtc agccctactt ctctctatta tgatgtcaac gttcagggta cccgcaatgt
     6181 gctggcggcc atggaaaaaa atggcgttaa aaatatcatc tttaccagtt ccgttgctgt
     6241 ttatggtttg aacaaacaca accctgacga aaaccatcca cacgatcctt tcaaccacta
     6301 cggcaaaagt aagtggcagg cagaggaagt gctgcgtgaa tggtataaca aagcaccaac
     6361 agaacgttca ttaaccatca tccgtcctac cgttatcttc ggtgaacgga accgcggtaa
     6421 cgtctataac ttgctgaaac agatcgctgg cggcaagttt atgatggtgg gcgcagggac
     6481 taactataag tccatggctt atgttggaaa cattgttgag tttatcaagt acaaactgaa
     6541 gaatgttgcc gcaggttatg aggtttataa ctacgttgat aagccggacc tgaacatgaa
     6601 ccagttggtt gctgaagttg aacaaagcct gaacaaaaag atcccttcta tgcacttgcc
     6661 ttacccacta ggaatgctgg gtggatattg ctttgatatc ctgagcaaaa ttacgggcaa
     6721 aaaatacgct gtcagctcag tgcgcgtgaa aaaattctgc gcaacaacac agtttgacgc
     6781 aacgaaagtg cattcttcag gttttgtg
//
LOCUS       DAJTMK010000196         6683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTMK010000196 DAJTMK010000000
VERSION     DAJTMK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.200"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            198..938
                     /locus_tag="OO135_003605"
     CDS             198..938
                     /locus_tag="OO135_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5399294.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(909..1676)
                     /locus_tag="OO135_003606"
     CDS             complement(909..1676)
                     /locus_tag="OO135_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5399295.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1882..2460)
                     /gene="lpcA"
                     /locus_tag="OO135_003607"
     CDS             complement(1882..2460)
                     /gene="lpcA"
                     /locus_tag="OO135_003607"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5399296.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGHEGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2700..5144
                     /gene="fadE"
                     /locus_tag="OO135_003608"
     CDS             2700..5144
                     /gene="fadE"
                     /locus_tag="OO135_003608"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5399297.1"
                     /translation="MMILSILATVVLLGALFYLRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5187..5633)
                     /gene="ivy"
                     /locus_tag="OO135_003609"
     CDS             complement(5187..5633)
                     /gene="ivy"
                     /locus_tag="OO135_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5399298.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5814..6584
                     /gene="yafV"
                     /locus_tag="OO135_003610"
     CDS             5814..6584
                     /gene="yafV"
                     /locus_tag="OO135_003610"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5399299.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT         1541 a         1704 c         1821 g         1617 t
ORIGIN      
        1 tcttaggaaa taaatgatac atgcgctttt cggactattg aaaactgccc ctgtgctaaa
       61 tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat gtgagccttc
      121 caaatccaga ctattccgat ttgagatcca ttttgccgtc ctggcgtgtg taaccgtttt
      181 atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt
      241 gtgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg agcaaagagt
      301 ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa gaacgtacgc
      361 tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat aaaatctgta
      421 aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt
      481 tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa gcgattaata
      541 ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac ctgatgatcc
      601 acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt gatgagatct
      661 tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg agtatttacc
      721 cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt aaggacttct
      781 gggagcaact gaagccgggt tacgactact ttgagcaaac ccgtaagcca ccgaccgttt
      841 ctgtggtcaa tggtcgttac gtggtcagca agccgttaag ccacgaagtt gtgcagccac
      901 aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc tggcataatc
      961 ttttgccagg tttcattgcc cgtcagcggc tgtgtcgcaa tcaccgtgac cacatcattc
     1021 ggtgtggtct gcgagctgaa gtcgatttct acatcctgat ccagcagcgt tgccacgcca
     1081 aacggtgcgc ggcgggtgat ccagtgcaga ttcgtcgagc aatacgccat tacatagcgc
     1141 ccatccgaaa gcagcatgtt gaaaacgccc ttctgccgca gttcgtccgc cagtgaagcg
     1201 atatatttaa acactgctgc catgttgccc ggcgtgcgcg ggtaacgctg cgttaattta
     1261 tgcagcagcc agcagaaggc tttttcgctg tcggtttcgc ctaccgggcg gaagttgccg
     1321 gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca gttacgcccc
     1381 cataactcgc gggtaaatgg gtgagtattt tccaacgcca cctcgccccg attagcctgg
     1441 cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag tttggcgatg
     1501 ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc ttcgtaaaag
     1561 gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgtac aagtccggtg
     1621 aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc gcacatgggt
     1681 aacctccaca acagcgggag cgcatggccc ccggcgaaga ttgatttaag aattttcagg
     1741 tcggatgcgg cgtaaacgaa ttattcggcc tacggttcgc gtacagattt gtaggcatga
     1801 taagacgcgt cagcatcgca tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc
     1861 cttatccggc ctacgccaga cttacttaac catctctttt tcaattaact ggatcaggat
     1921 atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt gcggtacgcg
     1981 aatttcgata tccgccgtgc cagccatttt gccgccgtct ttaccggtca gggtgatcac
     2041 tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag agttaccgga
     2101 ggtggagatc cccagcagta catcgccttc gtgacctacc gcttcaacgt agcgggagaa
     2161 aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt cagaaatagc
     2221 aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg
     2281 catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac cggctttaaa
     2341 gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt catcttttaa
     2401 aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat cctggtacat
     2461 gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat accgccaaaa
     2521 gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc ggataaaaac
     2581 agcaacaatg tgagctttgt tgtaattata ttgtaaacac attgctaaat gtatttacat
     2641 ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg cttttcgtta
     2701 tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc tatctccgcg
     2761 tgagcttatt tatcagcagt ctgattttgc tcgcctggac agccgccctc ggcgttgctg
     2821 gtctgtggtc ggcgtgggta ctggtgcctc tggccattat cctcgtgcca tttaactttg
     2881 cgcctatgcg taagtcgatg atttccgcgc cggtatttcg aggtttccgt aaggtgatgc
     2941 cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg tgggagggcg
     3001 acttgttcca gggcaagccg gactggaaaa agctgcataa ctatccgcag ccgcgcctga
     3061 ccgccgaaga gcaagcgttt ctcgacggcc cggtagaaga agcctgccgg atggcgaatg
     3121 atttccagat tactcatgag ctggcggatc tgccgccgga gttgtgggcg taccttaaag
     3181 agcatcgctt cttcgcgatg attatcaaaa aagagtacgg cggactggag ttctcagctt
     3241 atgcccagtc tcgcgtgctg caaaaactct ccggcgtaag cgggatcctg gcgattaccg
     3301 tcggcgtgcc gaactcatta ggcccgggcg agctgctgca acattacggc actgacgagc
     3361 agaaagatca ctatctgccg cgtctggcgc gtggtcagga gatcccttgc tttgcactga
     3421 caagcccgga agcgggttcc gatgcgggcg cgattccgga caccgggatt gtctgcatgg
     3481 gcgaatggca gggccagcag gtgctgggga tgcgtctgac ctggaacaaa cgctatatta
     3541 cgctggcacc gattgcgact gtgcttgggc tggcgtttaa actctccgac ccggaaaagc
     3601 tgctgggtgg cgcagaagat ttaggtatta cctgtgcgct gatcccgacc accacgccgg
     3661 gcgtggaaat tggtcgtcgc cacttcccgc tgaacgtacc gttccagaac ggaccgacgc
     3721 gcggtaaaga tgtcttcgta ccgatcgatt acatcatcgg tgggccgaaa atggccgggc
     3781 aaggctggcg gatgctggtg gagtgcctct cggtaggccg cggcatcacc ctgccttcca
     3841 actcaaccgg cggcgtgaaa tcggtagcgc tggcaacggg cgcgtatgct cacattcgcc
     3901 gtcagttcaa aatctctatc ggtaagatgg aagggattga agagccgctg gcgcgtattg
     3961 ccggtaatgc ctacgtgatg gatgctgcgg catcgctgat tacctacggc attatgctcg
     4021 gcgaaaaacc tgccgtgctg tcggctatcg ttaagtatca ctgtacccac cgcgggcagc
     4081 agtcgattat tgatgcgatg gatatcactg gtggcaaagg cattatgctc gggcaaagca
     4141 acttcctggc gcgtgcttac cagggcgcac cgattgccat caccgttgaa ggggctaaca
     4201 ttctgacccg cagcatgatg atcttcggac aaggagcgat tcgttgccat ccgtacgtgc
     4261 tggaagagat ggaagcggcg aagaacaatg acgtcaacgc gttcgataaa ctgttgttca
     4321 aacatatcgg tcacgtcggt agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg
     4381 gtttaaccag cagcacgcca accggcgatg ccactaaacg ctactatcag cacctgaacc
     4441 gcctgagcgc caacctcgcc ctgctttctg atgtctcgat ggcagtgctg ggcggcagcc
     4501 tgaaacgtcg cgagcgcatc tcggcccgtc tgggggatat tttaagccag ctctacctcg
     4561 cctctgccgt gctgaagcgt tatgacgacg aaggccgtaa tgaagccgac ctgccgctgg
     4621 tgcactgggg cgtacaagat gcgctgtatc aggctgaaca ggcgatggat gatttactgc
     4681 aaaacttccc gaaccgcgtg gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac
     4741 gtcattatct ggcaccttct gacaagctgg atcataaagt ggcgaagatt ttacaagtgc
     4801 cgaacgccac ccgttcccgc attggtcgcg gtcagtacct gacgccgagc gagcataatc
     4861 cggttggctt gctggaagag gcgctggtgg atgtgattgc cgccgaccca attcatcagc
     4921 ggatctgtaa agagctgggt aaaaacctgc cgtttacccg tctggatgaa ctggcgcaca
     4981 acgcgctggc gaaggggctg attgataaag atgaagccgc tattctggtg aaagctgaag
     5041 aaagccgtct gcgcagtatt aacgttgatg actttgatcc ggaagagctg gcgacgaagc
     5101 cggtaaagtt gccggagaaa gtgcggaaag ttgaagccgc gtaattgaat aacggagccg
     5161 aaaggctccg tttctttatc cgctaattat ttaaaattaa agccatccgg atggttttcc
     5221 aggctgccgg tcaacgccgc gaacagcacc gttttaccat caatcgaaag cgcatcgttc
     5281 acattcagcc aggtgagttt ctcttgcgac gttttctcat caatagtcga gaacagcccc
     5341 gtcatctgat tagatttctc ggaccacatc acagcgatac gttgcgagcc acagtcatgc
     5401 ggtttgcacg cgctcatcac ctgatacgtc tcatctccca acgttacggt ttgtgcggga
     5461 gtataagtac cgcctttcat cacccaggca ggcagcttat gcccttgtac catctgatta
     5521 aatgcagctt tggtggtttc gccctttgca aggctgctaa tggttaaatc atcctgcgcc
     5581 attgcactgg tggcgatgac cagagcggcg actgtcgtta ttgccttaaa catcattcct
     5641 cccgagctta tcctgcccat gttattaacc ttctaaagcc tggcgataac acgataattt
     5701 gcaaatcaga tatttccaat atcagcattt gttatcaatt tattctgcgc taaggcaact
     5761 gcctgataag gtgttaaagt tataaaaccc ttcgcataaa ggagttgtac accgtgcctg
     5821 gtttgaagat tacgcttttg cagcaaccac tggtgtggat ggatggccct gccaacctgc
     5881 gtcattttga tcgtcaactg gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga
     5941 tgtttaccag cggctttgcc atggaagcgg cagcttcgtc gctagcacaa gatgacgtag
     6001 tgaactggat gacagctaag gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat
     6061 tacaaacgga gtctggttcg gttaaccgct ttttgctggt tgagccgggc ggcacggtac
     6121 atttttatga taagcgtcat ctgttccgca tggcagatga gcatctacat tataaagcgg
     6181 gcaatgcgcg agtgattgtg gaatggcgcg gctggcgtat tttgccgttg gtgtgctacg
     6241 acttacgttt tccggtgtgg tcgcgcaatc tcaacgatta tgacctcgcc atctacgttg
     6301 ccaactggcc tgctccgcgc tctctgcact ggcaggcatt gctgacggcc cgcgcgattg
     6361 agaatcaggc gtatgtggcg ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt
     6421 atcgcggtga cagccgggtg attaatccac aaggcgagat tatcgctact gccgacgcgc
     6481 atcaggcaac gcgcattgat gcggagctgt cgatgatggc attgcgggaa tatagagaaa
     6541 agtttccggc atggcaggat gcggatgagt ttaggttgcg gtgagtgggg gttgtgttta
     6601 aggctggggg agagtcgggg taagactacg aaagcccgct ctccgcaagg actgacgcca
     6661 gatagtttct gtccatagct gct
//
LOCUS       DAJTMK010000197         6629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTMK010000197 DAJTMK010000000
VERSION     DAJTMK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.201"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..175
                     /gene="tnaA"
                     /locus_tag="OO135_003611"
     CDS             <1..175
                     /gene="tnaA"
                     /locus_tag="OO135_003611"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="tryptophan indole-lyase; catalyzes the formation of
                     indole and pyruvate from tryptophan; also has xysteine
                     desulfhydrase activity; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5399300.1"
                     /translation="AELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEP
                     KVLRHFTAKLKEV"
     gene            266..1512
                     /gene="tnaB"
                     /locus_tag="OO135_003612"
                     /pseudo
     CDS             266..1512
                     /gene="tnaB"
                     /locus_tag="OO135_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            1644..2819
                     /gene="mdtL"
                     /locus_tag="OO135_003613"
     CDS             1644..2819
                     /gene="mdtL"
                     /locus_tag="OO135_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5399301.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSITVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            2794..3753
                     /gene="yidZ"
                     /locus_tag="OO135_003614"
     CDS             2794..3753
                     /gene="yidZ"
                     /locus_tag="OO135_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5399302.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            3910..4659
                     /locus_tag="OO135_003615"
     CDS             3910..4659
                     /locus_tag="OO135_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5399303.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            4681..5247
                     /gene="yieF"
                     /locus_tag="OO135_003616"
                     /gene_synonym="chrR"
     CDS             4681..5247
                     /gene="yieF"
                     /locus_tag="OO135_003616"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5399304.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5301..6620)
                     /gene="adeP"
                     /locus_tag="OO135_003617"
     CDS             complement(5301..6620)
                     /gene="adeP"
                     /locus_tag="OO135_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5399305.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALMTSSLARVNWQDLTESVPAFITAVMMPFSF
                     SITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1589 a         1699 c         1644 g         1697 t
ORIGIN      
        1 ggctgaactg ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt
       61 cattattgaa gcctttaaac atgtgaaaga gaacgcggcg aatattaaag gattaacctt
      121 tacctacgaa ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat
      181 actacagagt ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa
      241 atagccttcc tctaaaggtg gcatcatgac tgatcaagct gaaaaaagca ctctgcattt
      301 tggggtgtta tggttatagc aggtacagta attggtggag gtatgtttgc tttacctgtt
      361 gatcttgccg gtgcctggtt tttctggggt gcctttatcc ttatcattgc ctggttttca
      421 atgcttcatt ccgggttatt gttattagaa gcaaatttaa attatcctgt cggctccagt
      481 tttaacacca tcaccaaaga tttaatcggt aacacctgga acattatcag cggtattacc
      541 gttgccttcg ttctctatat cctcacttat gcctatatct ctgctaatgg tgcgattatt
      601 agtgaaacga tatcaatgaa tttgggctat cacgctaatc cacgtattgt cgggatctgc
      661 acagccattt tcgttgccag cgtattgtgg ataagttcgt tagccgccag tcgcattacc
      721 tcattgttcc tcgggctgaa gattatctcc tttgtgatcg tgtttggttc tttcttcttc
      781 caggtcgatt actccatcct gcgcgatgcc accagcacca ctgcgggaac gtcttacttc
      841 ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat ttggtttcca cggcaatatt
      901 cccagcctga ttatttgcta tggaaaacgc aaagataagt taatcaaaag cgtggtattt
      961 ggttcgctgc tggcgctggt gatttatctc ttctggctct attgcacgat ggggaatatt
     1021 ccgcgcgaaa gctttaaggc gataatctcc tcaggcggca acgttgattc gctggtgaaa
     1081 tcgttcctcg gcaccaaaca gcacggcatt atcgagtttt gcctgctggt gttctctaac
     1141 ttagctgttg ccagttcgtt ctttggtgtc acgctggggt tgttcgatta tctggcggac
     1201 ctgtttaaga ttgataactc ccacggcggg cgtttcaaaa ccgtgctgtt aaccttcctg
     1261 ccacctgcgt tgttgtatct gatcttcccg aacggcttta tttacgggat cggcggtgcc
     1321 gggctgtgcg ccaccatctg ggcggtcatt attcccgcag tgcttgcaat caaagctcgc
     1381 aagaagtttc ccaatcagat gttcacggtc tggggcggca atcttattcc ggcgattgtc
     1441 attctctttg gtataaccgt gattttgtgc tggttcggca acgtctttaa cgtgttacct
     1501 aaatttggct aaatccttca agaagccagc cattcgctgg cttcttgcct ctcaggaaat
     1561 cacttatgtc caaatggcaa ctcgcctgat cctccttcac cacgtatgct ttgcgtcacc
     1621 ttactatcag gacgctttag cccatgtccc gctttttgat ttgtagtttt gccctggttt
     1681 tactttatcc cgccgggatt gatatgtacc tcgttggttt accgcgcatc gccgccgatc
     1741 tcaatgccag cgaagcgcag ttgcatattg cgttctccgt atatctggcg gggatggcag
     1801 ctgcgatgtt atttgccggt aaagtggccg atcgttcagg gagaaagccg gtcgccatac
     1861 ccggcgcggc gctatttatt attgcctcgg tgttctgttc actggctgaa accagcacgt
     1921 tatttcttgc aggccgattt ctacaggggt tgggcgcagg ctgttgttac gtagtggcgt
     1981 tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg tcattactca
     2041 acggtattac ctgcatcatt ccggtgttag cgccagtgct cggacatctg attatgctta
     2101 aattcccgtg gcagagtctg ttctgggcga tggcaatgat gggcatcgcg gtactgatgt
     2161 tgtctttgtt tattttaaaa gaaacgcgcc cagcgtcccc cgctgcttcg gacaaaccac
     2221 gagaaaatag cgagtcgctg cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc
     2281 tcagcgtttc ggtgatcctc actttcgtca atacatcgcc ggtattgctg atggaaatca
     2341 tgggttttga gcgcggagaa tacgccacca ttatggcgtt gaccgctggc gtcagcataa
     2401 ccgtttcatt ctccacgcca tttgcgctgg gaatttttaa gccacgtacg ttgatgatca
     2461 cctcacaggt gttattcctt gcagcgggga tcacccttgc cgtttcacct tcccatgcgg
     2521 tttctctgtt tggtatcaca ctgatttgcg ccggtttctc ggtaggtttt ggcgtagcga
     2581 tgagtcaggc gttaggacca ttttcattac gcgcgggcgt agccagctcg accttaggta
     2641 ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc agcggtggtt ggtatcggcg
     2701 catggaatat gctgatcggg attctgattg cctgtagcat agtgagcctg ttgctgatta
     2761 tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga aatccatcac cacgcttgat
     2821 ctcaatctgc tgctctgtct gcaactgctg atgcaggagc gcagcgtgac caaagcggcg
     2881 aagcggatga acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt aagagcgtgg
     2941 tttgacgacc cgctctttgt gaactcaccg ctggggctgt cgcccacacc gctaatggtc
     3001 agcatggagc aaaatctggc ggaatggatg caaatgagca atctgctgct ggataaaccg
     3061 caccaccaga caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc gctgatgatg
     3121 atcatgctta atgcgctgtc gaaacggatc taccaacgtt acccgcaggc gaccatcaaa
     3181 ctacgtaact gggattacga ttccttagat gccattactc gtggtgaagt ggatattggt
     3241 ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt taagctcgct accgttagcc
     3301 attgattatg aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa agatcatccg
     3361 gcactgcatg aaacgtggaa tctggacacc tttttacgtt atccgcatat cagcatttgc
     3421 tgggaacaga gcgacacctg ggcgctggac aatgtgttac aggagctcgg gcgcgaacgc
     3481 acgattgcta tgagcctgcc ggaatttgag cagtcactgt ttatggcagc gcaacccgac
     3541 aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact ccatcaactg
     3601 ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct ggaagttcct
     3661 tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg gttacgggaa
     3721 accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg cgacccaatt
     3781 tcacaataaa atgtaaaaaa gttgtaataa gcttgtctga atcgaacttt tagccgcttt
     3841 agtctgtcca tcattccagt aaatgattac tcttatattc ataatggacc attaagcatg
     3901 gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca tctgatttct
     3961 gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc
     4021 cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta tggcattggc
     4081 gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg
     4141 ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac aaccgaaggt
     4201 gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag cccacacgcg
     4261 cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct
     4321 aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact aaccggtgat
     4381 gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg cctgaaatgt
     4441 gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt gtggtccgtg
     4501 gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa acacagctgg
     4561 aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat gatgcgtttt
     4621 gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac aggagtcatt
     4681 atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat
     4741 ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt caatgcgtta
     4801 ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga aggttttcca
     4861 gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg
     4921 ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg gctttcccgc
     4981 ctgccggatc aaccgctggc cggtaaaccg gtgttgattc agaccagctc aatgggcgtg
     5041 attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct cgatgcaatg
     5101 gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga tcggcaaacc
     5161 ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac cgcatttggt
     5221 gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat gcatggcggg
     5281 tttttaatgc gctatcgatt ttaatgagcg tcgataaata caatcttcag gataaacagc
     5341 agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc aatcttcatc
     5401 acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct gaacggcatc
     5461 atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt cacgcgagca
     5521 agacttgagg tcatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     5581 gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag accggtacga
     5641 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     5701 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     5761 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     5821 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     5881 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     5941 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     6001 accgctgcgt gaatgttgcg cgaggccaga atagcaatga tgaagaagcc gaggataccc
     6061 agaagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccga gttagcgaca
     6121 atcacacctg cgtttttcag ccccatcatg ccgataaaca ggccgatacc gctggtaata
     6181 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     6241 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     6301 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     6361 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     6421 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     6481 aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc
     6541 gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc agaagcctgg
     6601 gttgtgtgtt gttgactcat tatctattt
//
LOCUS       DAJTMK010000198         6602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTMK010000198 DAJTMK010000000
VERSION     DAJTMK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.202"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..357
                     /locus_tag="OO135_003618"
     CDS             <1..357
                     /locus_tag="OO135_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399306.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1533
                     /locus_tag="OO135_003619"
                     /pseudo
     CDS             379..1533
                     /locus_tag="OO135_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1921..2297)
                     /gene="rnpB"
                     /locus_tag="OO135_003620"
     ncRNA           complement(1921..2297)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO135_003620"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2330..3475)
                     /gene="garK"
                     /locus_tag="OO135_003621"
     CDS             complement(2330..3475)
                     /gene="garK"
                     /locus_tag="OO135_003621"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5399307.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFRKIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVDASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTTL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3572..4462)
                     /gene="garR"
                     /locus_tag="OO135_003622"
     CDS             complement(3572..4462)
                     /gene="garR"
                     /locus_tag="OO135_003622"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5399308.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4492..5262)
                     /gene="garL"
                     /locus_tag="OO135_003623"
     CDS             complement(4492..5262)
                     /gene="garL"
                     /locus_tag="OO135_003623"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5399309.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5278..6576)
                     /gene="garP"
                     /locus_tag="OO135_003624"
     CDS             complement(5278..6576)
                     /gene="garP"
                     /locus_tag="OO135_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5399310.1"
                     /translation="MHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMG
                     YIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGIS
                     MFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLT
                     FAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKP
                     GSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKV
                     GLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNN
                     TTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGY
                     LVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1796 a         1720 c         1442 g         1644 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccaggaaa tatctacagg caatatcaat gctaattgtc cattgaaaga tatttacaat
      781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 aagatgaaag aattctcatc aaaaaaatat atgattacat cgatataatc attgaagatt
     1321 atatctgctc gtcccccaaa ttaattatac tatggattta aacatagaag actccagctc
     1381 agaagagtta ataccctcga gaaagacgaa aattcttcgg acaatggctt tgaagtcgaa
     1441 gaatataacg catatgaagc atataactca gagaatctat attttaccag agaggaatat
     1501 acgtataatt acgacctttt aaatgcaata tagacttaat aacttcttac aaaatctata
     1561 attaataccg tagatattca ttcatcttac cctgtgcacc aaaagcaaaa actcactgac
     1621 gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta
     1681 cgccatctct gcaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc
     1741 cgcatccggc atgaataaag cgcactcaac aatctgaaac ccgccggaac ggtttattac
     1801 gtacatcagg tgaaactgac cgataagccg ctttcttttg ggtatagtgt cgtggacagt
     1861 cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt tacgtaaatc
     1921 aggtgaaact gaccgataag ccgggttctg tcgtggacag tcattcatct aggccagcaa
     1981 tcgctcactg gctcaagcag cctacccggg ttcagtacgg gccgtacctt atgaacccct
     2041 atttggcctt gctccgggtg gagtttaccg tgccacggac tgttaccagc cgcgcggtgc
     2101 gctcttaccg caccctttca cccttacctg atcccgcttg cgcgggccat cggcggtttg
     2161 ctctctgttg cactggtcgt gggtttcccc cccaggcgtt acctggcacc ctgccctatg
     2221 gagcccggac tttcctcccc tccgcccgtc tcccccgaag aggacgacga cgaagcggcg
     2281 actgtctggt cagcttcggc gcgcagtata gagggtttgc gcgcccttgt caccccgcgt
     2341 tgcgcattcc aatcgccagt gtcgcggcga tattacgtga agcacggcag atattgtcat
     2401 aagccccgcg gaatgcttcg tccaacgtac ctatgctggt caatacgctg aagaccgcat
     2461 caatgccatg ctgatgtaca acgccaacat catcggtcag gctacccgca atgccaatca
     2521 ccggtttatg gtacttcttc gccacgtttg cgacaccaat cggtaccttg ccgtgaatac
     2581 tctggctgtc aatacgccct tcaccggtga tcaccagcgt acaatcgtga atatgttctt
     2641 ccagattcag cgtcgtagtg acgatttcaa taccactttt cagttccgca ccaagaaacg
     2701 ccatcagcgc cgcgcccata ccgcccgcag ctcctgcgcc ggggacatct ttcacatcaa
     2761 catgcagcgc ttttttaatg acctcggcat agtgagagag gttattgtcc agctcaacaa
     2821 tcatcgcttc actggctccc ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca
     2881 ccagcggatt ggtgacatca caagcgacgc gaatgacgca atcttttaag cgcggatcga
     2941 ggccggaaat atcaatatca ttcagagtat taagactacc gccgccaaaa ccaatttcat
     3001 tgccgttggc gtcgcataat ttcgccccca gcgcctgtac catgcctgcg ccgccatcat
     3061 ttgtagcgct gccgccaatg ccgataataa tgtttgtcgc accgctctcc agcgcctgca
     3121 ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta
     3181 ccagctccag cccactggcc gccgccattt caataaacgc ggttttgcca tcgccggaga
     3241 tcccccaact ggcatccact ttctcgccca acggccctgt aacccaggcg tgacgttcgg
     3301 aaccttgggt ggctgcaatc atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa
     3361 cagaaacgta ctgtgcatca ggaaaaattt tccgaaatcc tttttctatc gcctgcgcaa
     3421 cctcgctggc agataaactt tctttataag agtctggggc gattacgatt ttcataccta
     3481 tgcctgttac cacatgacgc cggagggcgt ttctcttatt cggcgtggat tccaggcccg
     3541 gattgcaata cgccatccgg gcacgacgtc attaacgagt aacttcgact ttcgccagtt
     3601 tttcgtagta gcacgccaga gcgctatgat ccgccgttcc taaaccatct gcacgcagtg
     3661 cctgcatcat ctccataacc gcagctgtga gcggcagttg cgcgccgacg ccgtgagaag
     3721 tatccagcgc attcgccaga tccttaatat gcagatcaat gcggaagccc ggcttgaagt
     3781 tgcggtccat caccatcggc gctttggcat ccagcacggt actgcccgcc agcccaccgc
     3841 gaattgcctg ataaaccagg tccgggttaa cgcccgcttt agttgccagc gttaacgctt
     3901 ctgacatcgc agcaatattc agcgccacaa tgacctgatt tgccagtttg gtgacgttac
     3961 ctgcaccgat ttccccggta tgcaccacgg aacccgccat cgctttcatc aaatcatagt
     4021 atttgtcgaa aatagccttg tcgccgccca ccatcactga cagcgtaccg tcgatggctt
     4081 tcggttcacc gccgctcacc ggagcatcca gcatatcaat gcctttcgct ttcagcgctt
     4141 cgctgatttc acggcttgcc agcggtgcga tagaactcat atcgatcaat accgtacctg
     4201 gcttcgcgcc ttcaataatg ccattctcac ccagcgccac ctctttcaca tgaggggagt
     4261 ttggcagcat ggttatgatg acgtcgcact gttcagcgat cgctttagcc gtagacgctg
     4321 tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg gttacggtca gcaaccacca
     4381 gcgagtaacc tgctttcaga aggtttttac tcattggttt acccataatc cccaggccaa
     4441 taaaaccaac tttcatagtc atatcaatca tctctcttgt tgcggtggtg gttatttttt
     4501 aaaggtatca gccagtttct gagtggcaga gcggaagacg ccaagatcgc tgccgacagc
     4561 cacaaacgtc gcgccccatt ccagataacg acgcgcatcg gcttcgaccg gcgcgagaat
     4621 accgctgggt ttgccgtgcg cactggcacg gttaaaaata tgctgaattg ctttttgtac
     4681 atccggatgc gaggcattgc cgagatggcc caatgccgca gccagatcgc tggggccgac
     4741 gaagataccg tctacacctt cggtagcggc aatagcatcg acgttatcta cgccctgctg
     4801 actttctatc tggaccagaa tagtgatgtt cttgttcgac tgagcgaaat aatccgccac
     4861 ggtgccaaac atattggcgc ggtgagaaac ggagacgccg cgaatgcctt ccggcgggta
     4921 acgagttgat gccaccgcct gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa
     4981 gttatagaaa ccgatatcca gaagacgctt aataattacc ggctcgttgg tcggcactcg
     5041 cactactggc gcgctggcgc tgcctttcag ggccattaac tgcggaataa acgtggaaat
     5101 atcgtttggc gcatgttcgc catccagcac cagccagtca aacccagcca aaccaagaac
     5161 ttcagtgcta atcgggttag agagtgctga ccagcaacca atttgtacct gtttcgcagc
     5221 cagtgcggct ttgaatttat tcgggaaaac atcgttattc atcgcttata cctttgctta
     5281 tttctgcaat tccatacgtt taatgtcgcc aactacgaag aggtagcaga ccatcgccat
     5341 cagcgctgaa catcccacga aaaccagtgc tgcattgaag gagtgcagtt cacttaccag
     5401 gtagccaatc accagtggag tgacaatgga ggcaacattg ccaaagacgt taaagacgcc
     5461 gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa atcaccggcc agcccagagc
     5521 accaaatcct ttgccaaaaa aagccagcgc catcagcatg accaccagcg tggtgttgtt
     5581 ggtgtagtta cataagatga tggtggaagc cagcaacatt cccagcacaa tcggtagctt
     5641 acgtgccagg gtcagggata aaccgcgttt gatcagataa tccgagaaga cacctcccag
     5701 cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg accagaccca ctttcagaat
     5761 cgacatgcct ttttcctgca ccagataaat cgggaaccag gtgaggaaga accaggtgat
     5821 ggtgttgata aaatattgtc cgaaaaatac gcccaacatc atgcggttag agagcaattg
     5881 cttgatgtaa tgcagtttgg gaccgcttgc tgctgcgctg cccggctttt tgtggtccat
     5941 atcgaccacc gcgccatttt cagagataaa cttcagctct tccgcagaca tacgtgggtg
     6001 atctgtcggg ttatgaatca acttgatcca cagcgccgtc agcacaaaac caatcacccc
     6061 cataacggtg aagacgtgct cccagcccca ggcgaaagtc agccagccaa gcagaggcga
     6121 aaagagcgcc agcgagaaat attgcgccga gttaaagatg gcggaggcag taccacgttc
     6181 tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc gggaatgatg gcgcttccga
     6241 gaagccgagc ataaagcgca taaagaacat agagatccct gcccaggcca gcgggaacat
     6301 atcaacaaag ccttgcagga aggtgaatag cgaccagaaa aagaggctgt aggtgtaaac
     6361 ttttttcgag ccaaacttat caagcagcca gccgccggga atttgcatca gcaggtaggc
     6421 ccagccaaaa gcggagaaga tgtaacccat cgaaaccgca cttaactgca actcttttgc
     6481 cacttcggta ccagcaatag acagcgttgc acgatcggcg tagttaacgg cggtaacaat
     6541 aaaaataatc agtaatatta aatagcgggt atgcacgcct ttctttttta cgtcaacggt
     6601 gt
//
LOCUS       DAJTMK010000199         6601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTMK010000199 DAJTMK010000000
VERSION     DAJTMK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.203"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            196..804
                     /locus_tag="OO135_003625"
     CDS             196..804
                     /locus_tag="OO135_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5399311.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            902..2293
                     /gene="selA"
                     /locus_tag="OO135_003626"
     CDS             902..2293
                     /gene="selA"
                     /locus_tag="OO135_003626"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5399312.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2290..4134
                     /gene="selB"
                     /locus_tag="OO135_003627"
     CDS             2290..4134
                     /gene="selB"
                     /locus_tag="OO135_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5399313.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4324..5475
                     /gene="yiaY"
                     /locus_tag="OO135_003628"
     CDS             4324..5475
                     /gene="yiaY"
                     /locus_tag="OO135_003628"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5399314.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5640..>6601
                     /gene="aldB"
                     /locus_tag="OO135_003629"
     CDS             5640..>6601
                     /gene="aldB"
                     /locus_tag="OO135_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5399315.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIY"
BASE COUNT         1564 a         1641 c         1833 g         1563 t
ORIGIN      
        1 gctgacaggc aaaaccctac agatttataa gaaagcctga agtgagatat ccatcagagt
       61 gaaataatta ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac
      121 aaaccatcaa cattttctgc cctttttccc atcaccatgc catactgata tttttcctcg
      181 cacaaaggac tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt
      241 tctattctgt tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac
      301 gcggacaacg gcgtggcgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa
      361 gagggcgaat gctggtttga ttcgccgatc atcgctgaat atattgagtt aatgaatgtc
      421 gctccggcga tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggcg
      481 ctggcggatg gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccggcg
      541 gcgcagcagt ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat
      601 gtgctggaag gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact
      661 atcgccattg cctgtgctgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt
      721 gtcgaccgcc cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct
      781 cgcaccgaac cgccaaaggc ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca
      841 atccgtggtt atgttaaacg cccttctccg tgtgagaggg ccttgattag ccaggtttcc
      901 tatgacaacc gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg
      961 cgatagctcc ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt
     1021 gcgtcagatg ctcgacgaag cgcgagaagt gattcgtgac agccagacgc ttcctgcgtg
     1081 gtgtgaaaac tgggcgcaag aagtcgatgc ccggttgacg aaagaggcgc agagcgcgct
     1141 gcgtccggtg atcaacctga cgggaaccgt gctgcatacc aaccttggac gagctttaca
     1201 ggcggaagcc gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta
     1261 cgatctggac gacgctggac gcggacatcg cgatcgggcg ctggcgcaac tgctgtgccg
     1321 tattacgggg gcggaagatg cctgtatcgt caataacaat gcggcggcgg tgttattgat
     1381 gttggcggcc actgccagcg ggaaagaggt ggtagtatct cgcggcgaat tggtggagat
     1441 tggcggcgcg tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt
     1501 agggaccacc aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc
     1561 actgttgatg aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga
     1621 tgaagcggaa ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg
     1681 cagtggctcg ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga
     1741 gttgattgcg gcgggcgtca gtctggtgag cttctccggc gacaagttgt taggcgggcc
     1801 gcaggcaggg attatcgttg gtaaaaaaga gatgatcgcc cgcctgcaaa gccacccgct
     1861 gaagcgtgca ttacgcgcgg ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct
     1921 ttatttacac cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag
     1981 cgcagaggtc attcaaatcc aggcacaacg tttacaggct ccccttgccg cacattacgg
     2041 cgcggagttt gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc
     2101 ggttgatcgc ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca
     2161 ccttgagtca ttagccgccc gctggcgtga attgccagtg ccggtgattg gtcgtattta
     2221 tgacggacga ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat
     2281 gttgttgaaa tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg
     2341 caggcgatta ctggcgtaaa tgctgaccgt ctgccggaag aaaaaaagcg cggcatgacc
     2401 atcgatctcg gctatgccta ctggccgcag ccggatggtc gcgtgcctgg ttttatcgac
     2461 gtacccggtc atgaaaagtt tctttccaac atgctggcgg gcgttggtgg tatcgatcac
     2521 gcgctgttgg tggtggcgtg cgatgacggc gtgatggcgc agacccgtga gcatctggcg
     2581 attttgcagc tgaccggtaa cccgatgctg acagtggcgc tgaccaaggc cgatcgcgtg
     2641 gacgaagcgc gtgttgatga ggttgaacgc caggtaaagg aggtgctgcg ggaatacggt
     2701 tttgctgagg caaaactgtt tatcaccgca gcaaccgaag gtcggggaat tgatgccctg
     2761 cgcgagcatc tgcttcagtt accaggacgt gaacacgcca gccaacatag tttccgcctc
     2821 gcgatcgacc gcgcatttac cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta
     2881 agcggggaag tgaaggtagg cgattcactc tggctgactg gtgtaaataa accgatgcgt
     2941 gtacgtgcgc tgcatgcaca aaaccagcca acagaaaccg cccatgccgg gcagcgtatc
     3001 gcgcttaaca tcgcgggtga tgcggaaaaa gagcagatta accgtggcga ctggctgctt
     3061 gccgatgcgc cgccagagcc gtttacacgg gtgattgtcg agcttcaaac ccatacaccg
     3121 ctgacccagt ggcagccgct gcatattcac cacgccgcca gccacgtcac gggacgcgtt
     3181 tcactgctgg aagataacct tgctgaactg gtcttcgaca ccccgttatg gctggcagat
     3241 aacgaccgcc tggtattgcg cgatatctct gcccgcaaca cgctggccgg agcgcgcgtc
     3301 gtgatgctta acccgccgcg tcgcggtaaa cgtaaaccag agtatctgca atggctggct
     3361 tccctcgcca gggcacagaa tgatagcgaa gcgttagccg ttcacctgca acgcggcgcg
     3421 gttaatcttc ctgatttcgc ctgggcgcgc cagctcaacg gcgaagggat gcgcgaactg
     3481 ctgcaacagc ctggctatat tcaggctggt tatagcttgt tgaatgcgcc ggttgccgct
     3541 cgctggcaac ggaaaattct cgacacatta gcgacttatc atgagcaaca tcgcgatgaa
     3601 cctgggcctg ggcgcgaacg tctgcgacgt atggcgttgc caatggaaga tgaagcgcta
     3661 gtactgttgc tgattgaaaa gatgcgcgaa agcggcgaca tcctcagtca tcacggttgg
     3721 ctgcatctgc cggatcacaa agcgggcttc agcgaagagc agcaggccat ctggcaaaaa
     3781 gcagagccgc tgtttggtga cgagccgtgg tgggtgcgtg atctggcaaa agagacgggt
     3841 accgacgagc aggcaatgcg cctgacgcta cgccaggcgg cgcagcaagg aataattacc
     3901 gcgatcgtca aagatcgtta ttaccgtaac gatcggattg tcgaatttgc caatatgatc
     3961 cgcgatctcg atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc
     4021 gtaggccgaa agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt
     4081 cgtcgtggaa atgatcattt attgcgcgac gcattattat ttccggaaaa ataaggaaat
     4141 gattaattaa tttttaaaat aaattaatac aaatttctta tgattttaaa aaaagcacat
     4201 tgtttaatga atacaatgtg ctttttatta gattaatttt gataattaat aataatgatc
     4261 atattttcga atgaaaatcc tcagtaagct tcccaccctt ttttacactt tcaggagtgt
     4321 gttatggcat cttcaacttt ctttattcct tctgtgaatg tcatcggcgc tgattcattg
     4381 actgatgcaa tgaatatgat ggcagattat ggatttaccc gtaccttaat tgtcactgac
     4441 agtatgttaa cgaaattagg tatggcgggc gatgtgcaaa aagcactgga agaacgcaat
     4501 atttttagcg ttatttatga tggcacccaa cctaacccca ccacggaaaa cgtcgccgca
     4561 ggtttgaaat tacttaaaga gaataattgc gatagcgtga tctccttagg cggtggttct
     4621 ccacacgact gcgcaaaagg tattgcgctg gtggcagcca atggcggcga tattcgcgat
     4681 tacgaaggcg ttgaccgctc tgcaaaaccg cagctgccga tgatcgccat caacaccact
     4741 gcgggtacgg cctctgaaat gacccgtttc tgcatcatca ctgacgaagc gcgtcatatc
     4801 aaaatggcga ttgttgataa acatgtcact ccgctgcttt ctgtcaatga ctcctctctg
     4861 atgattggta tgccgaagtc actgaccgcc gcaacgggta tggatgcctt aacgcacgct
     4921 atcgaagcat atgtttctat tgccgccacg ccgatcactg acgcttgtgc actgaaagcc
     4981 gtgaccatga ttgccgaaaa cctgccgtta gccgttgaag atggcagtaa tgcgaaagcg
     5041 cgtgaagcaa tggcttatgc ccagttcctt gccggtatgg cgttcaataa tgcttctctg
     5101 ggttatgttc atgcgatggc gtaccagctg ggcggtttct acaacctgcc acacggtgta
     5161 tgtaacgccg ttctgctgcc ccatgttcag gtattcaaca gcaaagtcgc cgctgcacgt
     5221 ctgcgtgact gtgccgctgc aatgggcgtg aacgtgacag gtaaaaacga cgcggaaggt
     5281 gctgaagcct gcattaacgc catccgtgaa ctggcgaaga aagtggatat cccggcaggc
     5341 ctacgcgacc tgaacgtgaa agaagaagat ttcgcggttc tggcgactaa tgccctgaaa
     5401 gatgcctgtg gttttactaa cccgatccag gcaactcacg aagaaattgt ggcgatttat
     5461 cgcgcagcga tgtaatcatc atttccacaa cggctggcaa attgtcagcc gctttttcaa
     5521 ctatctctgt aacccttgcc cgtaaattcg tgatagccgt cgtaaagctg ttaccgactg
     5581 gcgaagattt cgccagtcac gtctaccctt gttatacctc acaccgcaag gagacgatca
     5641 tgaccaataa tcccccttca gcacagatta agcccggcga gtatggtttc cccctcaagt
     5701 taaaagcccg ctatgacaac tttattggcg gcgaatgggt agcccctgcc gatggtgagt
     5761 attaccagaa cctgacgccg gtgactgggc agctgctgtg cgaagtggcg tcttcgggca
     5821 aacgagacat cgatctggcg ctagatgctg cgcacaaagt gaaagataaa tgggcgcaca
     5881 cctcggtgca ggatcgcgcg gcgattctgt ttaagattgc cgatcgaatg gaacaaaacc
     5941 tcgagctgtt agcgacagct gaaacctggg ataacggcaa acccattcgc gaaaccagtg
     6001 ctgccgacgt gccgctggcg attgatcatt ttcgctattt cgcctcgtgt attcgggcgc
     6061 aggaaggtgg gatcagtgaa gttgatagcg aaaccgtggc ctatcatttc catgaaccgt
     6121 taggcgtggt ggggcagatt atcccgtgga acttcccgct gctgatggcg agctggaaaa
     6181 tggctcccgc gctggcggcg ggcaactgtg tggtgctgaa acccgcacgt cttaccccgc
     6241 tttctgtact gctgctaatg gaaattgtcg gtgatttact gccgccgggc gtggtgaacg
     6301 tggtcaatgg cgcaggtggg gaaattggcg aatatctggc gacctcgaaa cgcatcgcca
     6361 aagtggcgtt taccggctca acggaagtgg gccaacaaat tatgcaatac gcaacgcaaa
     6421 acattattcc ggtgacgctg gagttgggcg gtaagtcgcc aaatatcttc tttgctgatg
     6481 tgatggatga agaagatgcc tttttcgata aagcgctgga aggctttgca ctgtttgcct
     6541 ttaaccaggg cgaagtttgc acctgtccga gtcgtgcttt agtgcaggaa tctatctacg
     6601 a
//
LOCUS       DAJTMK010000200         6554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTMK010000200 DAJTMK010000000
VERSION     DAJTMK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.204"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..517)
                     /locus_tag="OO135_003630"
     CDS             complement(<1..517)
                     /locus_tag="OO135_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR5399316.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGCGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWCSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSR"
     gene            complement(763..1214)
                     /locus_tag="OO135_003631"
                     /pseudo
     CDS             complement(763..1214)
                     /locus_tag="OO135_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1211..2266)
                     /gene="dinB"
                     /locus_tag="OO135_003632"
     CDS             complement(1211..2266)
                     /gene="dinB"
                     /locus_tag="OO135_003632"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5399317.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2337..3122)
                     /gene="lafU"
                     /locus_tag="OO135_003633"
     CDS             complement(2337..3122)
                     /gene="lafU"
                     /locus_tag="OO135_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5399318.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3094..4806
                     /locus_tag="OO135_003634"
                     /pseudo
     CDS             3094..4806
                     /locus_tag="OO135_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(5030..5527)
                     /gene="rayT"
                     /locus_tag="OO135_003635"
     CDS             complement(5030..5527)
                     /gene="rayT"
                     /locus_tag="OO135_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5399319.1"
                     /translation="MSEYRRYYIKGETWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5703..6452)
                     /locus_tag="OO135_003636"
     CDS             complement(5703..6452)
                     /locus_tag="OO135_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5399320.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHIGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARWILTEETIL"
BASE COUNT         1585 a         1705 c         1692 g         1572 t
ORIGIN      
        1 cccgcgagcc gctatgaacc aacagttgca gatgttgtgc atccagacct gctagtgcaa
       61 acagttcagc gttgatgatt tgatcaactt gttgcagttc agcgaaatga ttgccgccac
      121 cgatagagcc aagcgagctg caccacggat gctgtgcaaa tgctgacggc aggttttcct
      181 ccagccagct ttcttcagca acgtcatcca gatctgataa tcgcttttca aacttatcgg
      241 cgttgtattt gcgagcgaga atatctgttt gccatagcgc cataccgcag ccgatatcat
      301 tgccgaccag tgccggataa aaacgaccaa cagagaagaa cgctgcgcca atcggatagc
      361 cgcatccggg atgtaaatct ggcatcccca ctacgcgctg catgttgggt aaatttgccg
      421 tggtgtgtaa ttgttggatc gctaaactct cgatccactg gtcatcagat gcgatggtaa
      481 ataccgcatc ggataaggga cgaatataat tgcccatata ccattccgta aatagactcg
      541 tcatacttca agttgcatgt gttgcgacta gaattattga gagtaacaat taaaaaatca
      601 ggaaatggca gacgaatcca gggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa
      661 gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga ataagaaaac
      721 gcgcacatac taggcttgcg ttattttcat tgcaagctgg atttaatgtt gctgtttata
      781 acgcatataa aaattaataa accactctcc ccgacattca acgcgttgct gcttaactgt
      841 ctgaaaacca taacgttcaa aaaagggttt tgcggttatg cttgcgtcca ccgtaagttc
      901 ggattcagac ttaatcaaag gtttaacaaa gcgctggcaa ccccacggcg ggtgtattca
      961 gggtcaacaa ataacatatc gatatagtgt tcaacgcagg taataaagcc gaccagtttt
     1021 gcattaatga ctgcaacccg cacttgtgat ttcgcgagtt tctccttcca gcgagattcg
     1081 tcaatctgcg cccaggcggc aatttgttgt ggtgaataat gctgactggc agtcatcgta
     1141 accgctctaa tgaaaatagc gcatagttgc tgaaaatcgc caggctgata gtctcttatt
     1201 tgtatgttat tcataatccc agcaccagtt gtctttccat ttgcgggtca agcaacgtca
     1261 catgcagccc caccagtcgc acaccgcgcc cgccgcggcg ttcatcccag gttttacgcg
     1321 cggtggcgat taagtcagct ttattcagcc gcggccagac gtgctcctga gtggtttgct
     1381 gaaaatcatc aaactttaat ttcaccccct ggcgagcaat cagtaaatca ggtttcacct
     1441 ttgccagacg gcgttcaagt tccggataca gccgctcgat aatcgcttca cattcagacc
     1501 agtggtggat atcttccgcc atcgtgcgtt ccacgccgac ggattttcgc aaccgttcgc
     1561 tgttaacgtc gcgctcgtca atcccctgac tacgctccca caaaatgcgg ccaaatttgc
     1621 caaagcgttt aagcagcatc accagatcac acttttgtac atcaccgcag gttcgtagcc
     1681 ccatcgcttc cagttttgcc gccgagactt tgccgacgcc ggggattttt gccagtggta
     1741 aggtttgtaa aaatgccgga acttctgccg gcgtaatcac aaactggccg ttgggtttat
     1801 tcatgtcgga ggcgattttg gcgagaaact ttacgggtgc cacgcccgca gacgccgtca
     1861 gttgcagctc gttgaaaatc gtctggcgga tttcctgggc gatgagggtc gcagaaccgt
     1921 ggcaatggac gctatcggtg acgtcgagat aagcctcatc cagtgacaac ggttcaatac
     1981 gcgaggtgta gcgcgagaag atttcgcgga tatgatttga ggcttctttg taggcgtcaa
     2041 agcgccccgg aagcaaggtg agatgcgggc ataatttgag cgccatccct gtcggcatag
     2101 cgctacgtac gccaaattta cgcgcgggat aattggcggt actgatcacc ccccgacgtt
     2161 cgcggctgcc gccaatagca atagggatat cgcgcagggc gggattgtcg cgcatctcca
     2221 ccgccgcgaa aaagcagtcc atatccacat gaatgatttt acgcattgct cacctctcaa
     2281 cactggtaaa gtatacagtg atttcagagt ttgagaaatg cgtaaagatt cagcatttaa
     2341 cgcgttcgct gcgaaagcac ctgctgctta tccagctttt gcaccagcgg ctgcaccact
     2401 ttatccccat gctgaccaaa gtattgatac agcgtatcgg acgcactttt ggtcagcacc
     2461 ataatctcaa tgcgccggtt gcccgcgctt tgcggatttt tggaatccag cagcatctgg
     2521 tccgccattg cgcttacctg catcacttta tcttccggca ttccggcctc ttccagcacc
     2581 cgacgagccg aaagcgcgcg gtcacccgaa aggttccagt tgttgtagat attgtttttg
     2641 taagccatcg catcggtatg cccggtaata ataattttgt tatcgagcga gtcgaaaact
     2701 ggcgcgagct ccaccagcag cgttttaaag aacggcataa tctgcgcgct gccgcgttcg
     2761 aacatattgc gattctggtc gtctttaatc agcacgcgta atccctgggg aacaatctcc
     2821 atctccagat tcgcttccat atgcgcatca cgggcaatgg tggtgatact ggttgccagc
     2881 tcgccaagct cggtggcgga tttcttcttc agcagcgccg ttttctcatt gacgtcgcgc
     2941 gcttttttct ccgtttcttc cggcaccgcc acggtggcag gttttggcgg atgagacggg
     3001 ctgattttat ttaacggcga cagcccacca ccgttaaaaa tcgactgccc gtgcagcgcg
     3061 gcgataatgc tttcacgctc ggatttactg acgctgttga caatcaactt tatcgtcgtc
     3121 accaaagggg ccgagcgtat ttccgaggtt tcagcccgct ttaccctgga tgcgatgccc
     3181 ggtaagcaga tggcgattga cgccgatatt aatgccgggt tgatcaatca ggcgcaggca
     3241 caaacccgac gtaaagatgt tgccagcgag gccgatttct acggcgcgat ggaaggggca
     3301 tcgaaatttg tgcgcgggga tgctatcgcc gggatgatga ttctggcgat caacctgatc
     3361 ggcggcgtct gtatcgggat cttcaaatac aacctgagcg ccgacgccgc cttccagcag
     3421 tatgtgctga tgaccatcgg cgacgggctg gtggcgcaaa tcccttccct gctactctcc
     3481 accgcggcgg cgattatcgt cacccgcgtc agcgacaacg gcgatatcgc ccatgacgtg
     3541 cgtaaccagt tgctggcaag cccatcggtg ctctacaccg ctaccggaat tatgttcgta
     3601 ctggcggtag tgccgggaat gccacatctg ccgtttttgc tgttcagcgc cctgcttggc
     3661 tttaccggct ggcggatgag caaacgcccg caggcggcgg aagcggaaga gaaaagtctc
     3721 gaaacgctga cccgcaccat caccgaaacc agcgagcaac aggtcagttg ggaaaccatt
     3781 ccgcttatcg agcctatcag tttaagcctc ggctacaagc tggtggcgct ggtcgataaa
     3841 gcccagggca acccgctcac ccagcgtatt cgcggcgtgc aacaggtgat atccgacggc
     3901 aacggcgtgc tgctgccgga gatccgcatt cgggaaaact tccgccttaa gcccagccag
     3961 tacgctattt tcatcaacgg cattaaggct gatgaagcgg atattccggc ggataaactg
     4021 atggccctgc cctccagcga aacctacggc gagatagacg gcgtgctggg gaacgacccg
     4081 gcgtacggga tgccggtgac ctggatccag cctgcgcaga aagcgaaggc gctgaatatg
     4141 gggtatcagg tgatcgacag cgccagcgtg attgctaccc atgtaaataa gattgtgcgc
     4201 agctatattc ctgatttatt taactatgac gatattacgc agttgcataa ccgtttgtcg
     4261 tctatggcac cgcgcctggc ggaggattta agcgcggtgc tcaattacag ccagttgctg
     4321 aaagtgtacc gggcgctgct gaccgaaggc gtttccctgc gcgatatcgt caccatcgcc
     4381 accgtgctgg tcgccagtag cgcggtgacc aaagatcata ttctgctggc agccgatgtg
     4441 cgcctggcgc tgcggcgcag cattacccat ccgttcgttc gcaagcagga gctgacggtg
     4501 tataccctga ataatgagct ggaaaatctg ctgactaacc tagtgaatca ggcgcaacag
     4561 ggcggtaaag tgatgctcga cagcgtgcca gtggacccga atatgctcaa ccagttccag
     4621 agtacgatgc cacaggtgaa agagcagatg aaagcggcgg ggaaagaccc ggtgctgctg
     4681 gtgccaccgc agctgcgccc tttgctggcg cgttatgcaa ggttgtttgc tccggggctg
     4741 catgtgctgt cgtataacga agtgccggac gagctggagt tgaagattat gggggcgttg
     4801 atgtaagtat ccagcattgg tgcacaactg ccggatgcgg cgtgaacgcc ttatccggcc
     4861 taccgatcca gcacaggttt gtaggcatga taagacgcgc cagcgtcgca tcaggcatct
     4921 gcgcataacc gccggatgcg gcataaacgc cttatccggc ctaccgatcc ggcacaggtt
     4981 tgtaggcatg ataagacgcg ccagcgtcgc atcaggcatc tgcgcataat tataaaatga
     5041 tacgctcgcc agcatttata tccgttacgt cccccgccca atcgatggga tataaccctc
     5101 gcgcgacatc gcgatggaac gttgagaatg gccaatcact cacttgcttt acccaaccat
     5161 gctttactgg atttatataa atataatcaa catgatgcct ataatctttg gtattgcgga
     5221 tggcgtgctc ccaaaaacgt ggttgccaga tatttttcaa tccacaagca tgggtaaatt
     5281 gctttttaat ttcccgccag cgagaggaaa aatcatcatc gccttcaggt aatgtccata
     5341 tgcaatgcat atgctctggc aaaacgaccc aggcgttgat ttcaaaaggc ctgtctcgct
     5401 taactttaat aatggcgtta cggagcatct ggtactgggt ggtcaagagt tggcttcgac
     5461 gattgcgtaa attcaccgtg aaaaaccagg tttccccctt gatgtaataa cgacgatatt
     5521 cagacatggc gactcctttt tctcaacggt aagaagaaaa ggaaaggtcg gcaatgaggt
     5581 gatggaataa caggaaagcg gctcgcaaaa acgcacagat ttgtaggcat gataagacgc
     5641 gtcagcgtcg catcaggcat ctgcgcacca ccgccggatg cggcgtaaac gccttatccg
     5701 tcctacaaaa tcgtctcttc cgtcaatatc caccgcgcgc ccaaaaaatg atcctgccag
     5761 aaaggttcag ctaaccgact tacccgaatg gtttcccccg tgcgcggcga ctcaataaac
     5821 tgaccgtctc ccagatacac gccaatatgg tcggcaattt cacggctgtg gatatggaaa
     5881 aacagcaaat ctccccggcg caggtcgtta tttgccacaa tggttgcccg atggtagtga
     5941 tacatctcat tagccgtgcg cgggagctta gcctcaagga tcttgttgta ggcataaaaa
     6001 accaacccgc tacagtcgaa gccttgatcg ggccgcgtgc cgccccagac gtaaggcttg
     6061 ccaagctgtt gctccagacg gtgaatcgcc acttcggtga tattatgcag gtggttgctg
     6121 ctgaggaaat gattgttttc taccaacgcc tgccagcgtc tgtcacttgc cttgagcggc
     6181 gcaggaaacc attctggatg ctgttttatc tgctcccggt tgtgctggcg tagcgccctt
     6241 ctgctttcgg cattgccttc cacaatgtag ctggcctgtt ttttcagatg ggtttggtac
     6301 tgttttaata aacgcgcacg gttttgcatc cgctggcggg cggcgtagct gaaagagata
     6361 tgtgatgtgg tggcgctggc gagtaaaggg agcaaaacgc ataacgaaaa cacgcctgga
     6421 tgcagaaatc tgccgagcaa gaacgaagac atgaaggaca taaaccggct gaatgaaggt
     6481 gagaatttta aaagaccgga ggagtaaacg ggattattaa ttgcttattt gcatattact
     6541 attgaggttt cata
//
LOCUS       DAJTMK010000201         6534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTMK010000201 DAJTMK010000000
VERSION     DAJTMK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.205"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..858
                     /gene="ag43"
                     /locus_tag="OO135_003637"
     CDS             <1..858
                     /gene="ag43"
                     /locus_tag="OO135_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5399321.1"
                     /translation="HDNNGGIARGATPESSGSYGLVRLEGDLLRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGIQAGYAHSISGSSAEGYN
                     GQATLNVTF"
     gene            930..1163
                     /locus_tag="OO135_003638"
     CDS             930..1163
                     /locus_tag="OO135_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5399322.1"
                     /translation="MPGCTSRLLPEGPFSREQVVAVTTVYRNVFTEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNVWLASEGLLRQ"
     gene            1254..2072
                     /locus_tag="OO135_003639"
     CDS             1254..2072
                     /locus_tag="OO135_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5399323.1"
                     /translation="MRLASRFGYANQIRRDRPLTREELMQVVPSVFGEEKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2164..2649
                     /locus_tag="OO135_003640"
     CDS             2164..2649
                     /locus_tag="OO135_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5399324.1"
                     /translation="MTTFSHSSTTPSVSVTAASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDEDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            2664..3140
                     /locus_tag="OO135_003641"
     CDS             2664..3140
                     /locus_tag="OO135_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5399325.1"
                     /translation="MQQISFLPGEMTTRERSLIQRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            3227..3448
                     /locus_tag="OO135_003642"
     CDS             3227..3448
                     /locus_tag="OO135_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5399326.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3528..3896
                     /locus_tag="OO135_003643"
     CDS             3528..3896
                     /locus_tag="OO135_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5399327.1"
                     /translation="MSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKT"
     gene            3986..4363
                     /locus_tag="OO135_003644"
     CDS             3986..4363
                     /locus_tag="OO135_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5399328.1"
                     /translation="MKTLSDTHIREASRCPSPVTVWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTNSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKYQEAKR"
     gene            4360..4539
                     /locus_tag="OO135_003645"
                     /pseudo
     CDS             4360..4539
                     /locus_tag="OO135_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4588..4782
                     /locus_tag="OO135_003646"
     CDS             4588..4782
                     /locus_tag="OO135_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCR5399329.1"
                     /translation="MPLTTETALDILIAWLQDNIDCESGIIFDNDENKTDSVTLLPCI
                     KQVRQDVRTLRHMQLLHQNR"
     gene            4867..5712
                     /locus_tag="OO135_003647"
     CDS             4867..5712
                     /locus_tag="OO135_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5399330.1"
                     /translation="MSDITASRPEVVNGHTAVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINIFRGLS
                     WNYKNNSPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIVAKHYPG
                     QLSAI"
     gene            complement(5812..5887)
                     /locus_tag="OO135_003648"
     tRNA            complement(5812..5887)
                     /locus_tag="OO135_003648"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5852..5854),aa:Ile,seq:cat)
     gene            6012..6518
                     /gene="mug"
                     /locus_tag="OO135_003649"
     CDS             6012..6518
                     /gene="mug"
                     /locus_tag="OO135_003649"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR5399331.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         1586 a         1754 c         1728 g         1466 t
ORIGIN      
        1 cacgataaca acggtggtat tgcccgtggg gccacgccgg aaagcagcgg cagctatggc
       61 ctcgtccgtc tggagggtga cctgctcaga acagaggttg ccggtatgtc tgtgaccgcg
      121 ggggtatatg gtgctgctgg ccattcttcc gttgatgtta aggatgatga cggctcccgc
      181 gccggcacgg tccgggatga tgccggcagt ctgggcggat acctgaatct ggtacacaca
      241 tcctccggcc tgtgggctga cattgtggca cagggaaccc gccacagcat gaaagcatca
      301 tcggacaata acgacttccg cgcccggggc tggggctggc tgggctcact ggaaaccggt
      361 ctgcccttca gtatcactga caatctgatg ctggagccac aactgcagta cacctggcag
      421 ggactctccc tggatgacgg ccaggataac gccggttatg tgaagttcgg gcatggcagt
      481 gcacaacatg tgcgtgccgg cttccgtctg ggcagccaca acgatatgaa ctttggtaaa
      541 ggcacctcat cccgtgacac cctgcgcgac agtgccaaac acagtgtgcg tgaattgccg
      601 gtgaactggt gggtacagcc ttctgttatc cgcaccttca gctcccgggg agacatgagc
      661 atgggtacag ccgcagccgg cagtaacatg acgttctcac cgtcccggaa tggcacgtca
      721 ctggacctgc aggccgggct ggaagcccgt gtccgggaaa atatcaccct gggcattcag
      781 gccggttatg cccacagtat cagcggcagc agcgctgaag gctataacgg tcaggccacg
      841 ctgaatgtga ccttctgatt aatcaggtca ctccccttca ggccccggtc attatgaccg
      901 gggcctttta ctttcaggga gtaacagata tgccgggttg tacatcacgt ttattgcccg
      961 aaggcccttt ctcacgcgaa caggtggttg ctgttacaac agtttaccgc aatgtgttta
     1021 ctgaagatga ccagggaacg catttccggc tggttatccg taatgctgaa ggtcagctac
     1081 gctggcgctg ctggaatttt gagcccgatg ccggcaaaca acttaatgtg tggctcgcca
     1141 gtgaagggct tctcagacaa taaacgtcat tcatttcccc catcaggccg cgtcttctcc
     1201 gggagacgcg gctttttcat ttataccgct aactcattca taaggaataa atcatgcgat
     1261 tagccagtcg ttttggttat gcgaaccaga tacgccgtga ccgcccctta actcgtgaag
     1321 aactgatgca ggtcgtcccc agcgtattcg gggaagagaa acacacatcc agaagcgaga
     1381 actatacctg gatccccacc atcaccgtgc tggaaagcct gcagcgggaa ggcttccagc
     1441 cattcttcgc ctgccagacc cgcgtgcgcg acccgggccg ccggggatac accaaacaca
     1501 tgctgcgtct gcgccgtgac ggggagataa acggacaaca tgttcctgaa atcatcctgc
     1561 tgaactccca tgacggtacc tccagctacc agatgctgcc gggttacttc aggttcgtct
     1621 gccagaacgg atgcgtctgt ggtcagtctc tgggggaagt gcgtgttcca caccggggag
     1681 acgtagtgga gaaagttatc gaaggggctt acgaggtggt gggggttttt gaccggattg
     1741 aggagaagcg tgatgccatg cagtctctgg ttctgccgcc accggcacgc caggcgctgg
     1801 cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc accgctgaca
     1861 ttctgacgcc acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca
     1921 tccaggaaaa tatgctgaaa ggcgggattt ccggtcgcag cgctaaagga aaacgtatcc
     1981 acacccgtgc cattcacagc atcgacaccg atattaagct caaccgcgca ttgtgggtga
     2041 tggcagaaac gctgctggag agcatgcgct gatgctgttt ccctgaaagt acagccctga
     2101 ctcacgtctg taccagtccc ttggcattct tccattcatt tactttttat aaggaatcat
     2161 ctcatgacaa ccttttcgca tagctccacc acaccttctg tttccgtaac cgctgcatca
     2221 gggaataacc agtctcaact ggttgccacc atagtccctg atgaacagcg catcagcttc
     2281 tggccgcagc actttggcct cattccacag tgggtgaccc tggagccccg tgtcttcggc
     2341 tggatggacc gtctgtgtga agactactgc ggtggtatct ggaatctgta caccctgaat
     2401 aacggcggcg catttatggc gcccgaaccg gatgaagatg atggagaaac ctgggtactg
     2461 ttcaatgcca tgaacggtaa ccgcgctgaa atgagcccgg aagccgccgg cattgccgcc
     2521 tgtctgatga cgtacagcca tcatgcctgt cgtacggaga attatgccat gacggtccat
     2581 tattaccggt tgcgggatta cgccctgcag catccggaat gcagcgccat tatgcgcatc
     2641 attgactgaa aggggccgga ataatgcaac agatttcctt tctgcccgga gaaatgacaa
     2701 ccagagagcg cagcctcatt cagcgggccc tgaaaaccct ggaccgccat cttcatgagc
     2761 ccggcgtggc cttcacctcc acgcatgcgg cccgggaatg gctgattctg aacatggcag
     2821 gactggagcg ggaagagttc cgggtactgt atctgaacaa ccagaatcag ctgattgccg
     2881 gtgaaaccct cttcaccggc accatcaacc gcacggaagt tcatccccgg gaggtgatta
     2941 aacgcgcgct gtaccacaat gccgctgccg tggtgctggc acacaatcac ccgtccggtg
     3001 aagtcacacc cagtaaggca gaccggctta ttacggaacg tctggttcag gcgctggccc
     3061 tggtggatat ccgggtaccg gaccatctga tagtcggtgg cagccaggta ttctcctttg
     3121 cggaacacgg tctgctttaa cccgtcaccg tcacaatcac cttcatatca cttcaatttc
     3181 tctttctcag ctgtttctta ctttcacatt caggaggact attctcatga aaatcatcac
     3241 ccgtggtgaa gccatgcgta ttcaccgtca gcatcctgca tcccgtcttt ttccgttctg
     3301 taccggtaaa taccgctggc acggtagcac ggatacatat accggccgtg aagtacagga
     3361 tattcccggt gtgctggctg tgtttgcaga acgccgtaag gacagttttg gtccgtatgt
     3421 ccggctgatg agcgtcaccc tgaactgagt ctggaagggc attcagaaga gcagaattat
     3481 caccaccacc ggaccattct taaccaattt tctgtgagga ttttatcgtg tcagacacac
     3541 tccccgggac aacgcatccc gacgataaca acgaccgccc ctggtggggg ctaccctgca
     3601 ccgtgacgcc ctgttttggg gcacgtctgg tgcaggaggg taaccggttg cattaccttg
     3661 ccgaccgcgc cggtatcaga ggccggttca gcgacgcgga tgcgtaccat ctggaccagg
     3721 cctttccgct gctgatgaaa caactggagc tcatgctcac cagcggtgaa ctgaatcccc
     3781 gccatcagca taccgtgaca ctgtatgcaa aagggctgac ctgcgaagcc gacaccctcg
     3841 gcagttgtgg ctacgtttat atggctgttt atccgacgcc cgaaacgaaa acgtaaatca
     3901 tcagaataat cttctgctcc ggcctggtgt tttcaccacg ccactttccc atttttcatc
     3961 tctgcatatc aggaaaatct tcagtatgaa aacattatcc gacacacaca tacgggaggc
     4021 atcgcgctgc ccgtctcccg tcaccgtctg gcagacactg ctcacccgac tgctggacca
     4081 acactacggc ctcacgctga atgacacacc gttcgctgat gaacgtgtga ttgagcagca
     4141 tattgaggca ggcatttcac tgtgcgatgc ggtgaacttt ctcgttgaaa aatacgcgct
     4201 ggtacgtacc gaccagccgg gattcagcgc ctgtaccaac tctcagttaa taaacagcat
     4261 cgatatcctc cgggctcgcc gggcgactgg cctgatgacc cgcgacaatt acagaacggt
     4321 aaataacatt acccagggta agtatcagga ggcgaaacga tgaaactggc cctgacgctg
     4381 gaagctgaca gcgttaacgt acaggcactg aacatggggc gaatcgtcgt tgacgtcgat
     4441 ggtattgagc tcgctgaact gattaacgaa gtcgctgaaa acggttattc actccgcatg
     4501 gtggatgaac gtggcgctac cgaaacaccg gcgacttctg ccagccctca tcagttgccg
     4561 gaagacaata cagtgataca gagcgcgatg ccattaacca ctgaaacagc gctggatatc
     4621 ctcattgcgt ggctgcagga caatatcgac tgcgagtccg gaattatttt cgacaacgac
     4681 gagaataaaa cggattcagt tacactgtta ccctgtatta aacaggtgcg acaggatgtc
     4741 cgtaccctgc gccatatgca acttctgcac cagaaccggt gaatctcact catcagactt
     4801 cattccactg acgccagcct gaacagggct ggcgttttca tttatctgca aaaaggaata
     4861 tcgattatgt ctgatatcac agcctcccgt ccggaagtgg tcaacgggca tacggccgtt
     4921 atctgctcaa ccagtatccg ccacattctg gctgtacgaa aaagtacact gctgcaaatc
     4981 gacacactta tccggcaact ggctgaaatc tcagcaatga cagaaagtat tggcggtaaa
     5041 accgcactgg actgggccat gaaacagaat tttcgctgcg gctgctggct gatggagaaa
     5101 cctgaaacgg caatgaaagc catcacccgc aaccttgacc gcgaaatctg gcgtgacctg
     5161 atgcaacgtt ccgggatgct gtcattaatg gatgcgcagg cccgtgatac atggtaccgg
     5221 tcactggagt acgataattt tccggaaatc agtgaagcga acattctgag cacatttgaa
     5281 caactgcacc agaataagga tgaggtgttt gagcgagggg taatcaatat tttcagggga
     5341 ctgagctgga attacaaaaa caattcaccc tgtaaatttg gcagtaaaat tatcgtcaac
     5401 aatctggtga ggtgggacag atggggattt catctgaaca acggcccaca ggccgatcga
     5461 cttgccgacc tggaaagaat gctgcatctg ttcagcggca aaccgatccc cgacaaccgg
     5521 gaaaacatca ctattcgtct ggatgatcac atccagtctg ttcagggtaa agaggactat
     5581 gaagatgaga tgttcagcat cagatacttt aagaagggct ccgcgcatat cacgttcaga
     5641 aaaccagaac tggttgatcg gctaaatgac atcgttgcga agcattatcc ggggcaacta
     5701 tcagccatct gagtcagatg gagaaaaagc ttacctgacc cattacctga cccaattcaa
     5761 aggcaaaaga aaaagggtta gctgatttat tagctaaccc tttgttgtat atggtggccc
     5821 ctgttgggtt tgaaccaacg accaagcgat tatgagtcgc ctgctctaac cactgagcta
     5881 aggggccatg gtagcggatt ataaagtaac tccgtgtcgc aatccagcca ttaccgtgcg
     5941 cctgctgttt ttataaacaa tgtattttca atccgttata cttttcctgg tgatgttaga
     6001 aaggagtaaa tatggttgag gatattttgg ctccagggtt acgggtcgtg ttttgcggta
     6061 tcaaccctgg gctttcatcc gccgggactg gttttccctt tgctcatcca gcaaatcgct
     6121 tctggaaggt gatatatcag gccgggttta ccgaccgtca gttgaagccg caggaggcac
     6181 agcatctgct ggattatcgt tgtggcgtca ccaaactggt agaccgtcca acggtgcaag
     6241 ccaatgaagt ttcaaagcag gagctgcacg caggtgggcg taagctgatt gaaaaaattg
     6301 aagattatca gccgcaggcg ttggcgattc tgggcaaaca agcatatgaa cagggattca
     6361 gccagcgcgg tgcacagtgg gggaaacaaa cgctcaccat tggttcgacg cagatttggg
     6421 tgctgccaaa tcccagcggt ttaagtcgcg tttcactaga gaaactggtt gaagcgtatc
     6481 gcgagctgga ccaggcgctg gtagtgcgtg ggcgataaaa aacgccaccg aaaa
//
LOCUS       DAJTMK010000202         6526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTMK010000202 DAJTMK010000000
VERSION     DAJTMK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.206"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            370..1161
                     /gene="nanR"
                     /locus_tag="OO135_003650"
     CDS             370..1161
                     /gene="nanR"
                     /locus_tag="OO135_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5399332.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            1283..2176
                     /gene="nanA"
                     /locus_tag="OO135_003651"
     CDS             1283..2176
                     /gene="nanA"
                     /locus_tag="OO135_003651"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5399333.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            2285..3775
                     /gene="nanT"
                     /locus_tag="OO135_003652"
     CDS             2285..3775
                     /gene="nanT"
                     /locus_tag="OO135_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5399334.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVYILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            3823..4512
                     /locus_tag="OO135_003653"
     CDS             3823..4512
                     /locus_tag="OO135_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5399335.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            4509..5384
                     /gene="nanK"
                     /locus_tag="OO135_003654"
     CDS             4509..5384
                     /gene="nanK"
                     /locus_tag="OO135_003654"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5399336.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            5381..5845
                     /locus_tag="OO135_003655"
     CDS             5381..5845
                     /locus_tag="OO135_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5399337.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(5921..6226)
                     /locus_tag="OO135_003656"
     CDS             complement(5921..6226)
                     /locus_tag="OO135_003656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399338.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(6243..6526)
                     /locus_tag="OO135_003657"
                     /pseudo
     CDS             complement(6243..6526)
                     /locus_tag="OO135_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1507 a         1606 c         1750 g         1663 t
ORIGIN      
        1 gtgtcaaaag aaaactgtca atcccataaa cggcagattg aaaacaatga tgttatattt
       61 tttgcaagtc tatttatggt gcggatgtcg tgtttttaat tgtaggtgag gtaatttttc
      121 attaaaaaaa catgcgctta tgattatttt gcaagaacgc attcataata ttcataatgc
      181 tcgtgaatag tcttataaat aattcaaacg ggatgttttt atctgcgtta cattaatttt
      241 tcgcaatagt taattattcc gttaattatg gtaatgatga ggcacaaaga gaaaaacctg
      301 ccattttccc ctactttcaa tcctgtgata ggatgtcact gatgatgtta atcacactga
      361 ccttacagaa tgggccttat gaacgcattt gattcgcaaa ccgaagattc ttcacctgca
      421 attggtcgca acttgcgtag ccgcccgctg gcgcgtaaaa aactctccga aatggtggaa
      481 gaagagctgg aacagatgat ccgccgtcgt gaatttggcg aaggtgaaca attaccgtct
      541 gaacgcgaac tgatggcgtt ctttaacgtc gggcgtcctt cggtgcgtga agcgctggca
      601 gcgttaaaac gcaaaggtct ggtgcaaata aacaacggcg aacgcgctcg cgtctcgcgt
      661 ccttctgcgg acactatcat cggtgagctt tccggcatgg cgaaagattt cctttctcat
      721 cccggtggga ttgcccattt cgaacaatta cgtctgttct ttgaatccag tctggtgcgc
      781 tatgcggctg aacatgccac cgatgagcaa atcgatttgc tggcaaaagc actggaaatc
      841 aacagtcagt cgctggataa caacgcggca ttcattcgtt cagacgttga tttccaccgc
      901 gtgctggcgg agatccccgg taacccaatc ttcatggcga tccacgttgc cctgctcgac
      961 tggcttattg ccgcacgccc aacggttacc gatcaggcac tgcacgaaca taacaacgtt
     1021 agttatcaac agcatattgc gatcgttgat gcgatccgcc gtcatgatcc tgacgaagcc
     1081 gatcgtgcgt tgcaatcgca tctcaacagc gtctctgcta cctggcacgc tttcggtcag
     1141 accaccaaca aaaagaaata atgccacttt agtgaagcag atcgcattat aagctttctg
     1201 tatggggtgt tgcttaattg atctggtata acaggtataa aggtatatcg tttatcagac
     1261 aagcatcact tcagaggtat ttatggcaac gaatttacgt ggcgtaatgg ctgcactcct
     1321 gactcctttt gaccaacaac aagcactgga taaagcgagt ctgcgtcgcc tggttcagtt
     1381 caatattcag cagggcatcg acggtttata cgtgggtggt tcgaccggcg aggcctttgt
     1441 acaaagcctt tccgagcgtg aacaggtact ggaaatcgtc gccgaagagg cgaaaggtaa
     1501 gattaaactc atcgcccacg tcggttgcgt cagcaccgcc gaaagccaac aacttgcggc
     1561 atcggctaaa cgttatggct tcgatgccgt ctccgccgtc acgccgttct actatccttt
     1621 cagctttgaa gaacactgcg atcactatcg ggcaattatt gattcggcgg atggtttgcc
     1681 gatggtggtg tacaacattc cagccctgag tggggtaaaa ctgaccctgg atcagatcaa
     1741 cacacttgtt acattgcctg gcgtaggtgc gctgaaacag acctctggcg atctctatca
     1801 gatggagcag atccgtcgtg aacatcctga tcttgtgctc tataacggtt acgacgaaat
     1861 cttcgcctct ggtctgctgg cgggcgctga tggtggtatc ggtagtacct acaacatcat
     1921 gggctggcgc tatcagggga tcgttaaggc gctgaaagaa ggcgatatcc agaccgcgca
     1981 gaaactgcaa actgaatgca ataaagtcat tgatttactg atcaaaacgg gcgtattccg
     2041 cggcctgaaa actgtcctcc attatatgga tgtcgtttct gtgccgctgt gccgcaaacc
     2101 gtttggaccg gtagatgaaa aatatctgcc agaactgaag gcgctggccc agcagttgat
     2161 gcaagagcgc gggtgagttg tttcccctcg ctcgccccta ccgggtgagg ggaaataaac
     2221 gcatctgtac cctacaattt ttataccaaa gcgtgtgggc atcgcccacc gcgggagact
     2281 cacaatgagt actacaaccc agaatatccc gtggtatcgc catctcaacc gtgcacaatg
     2341 gcgcgcattt tccgctgcct ggttgggata tctgcttgac ggttttgatt tcgttttaat
     2401 cgccctggta ctcaccgaag tacagggtga attcgggctg acgacggtgc aggcggcaag
     2461 tctgatctcc gcagccttta tctctcgctg gttcggcggc ctgatgctcg gcgctatggg
     2521 tgaccgctac gggcgtcgtc tggcaatggt caccagcatc gttctcttct cggccgggac
     2581 gctggcctgc ggctttgcgc caggctacat caccatgttt atcgcgcgtc tggtcatcgg
     2641 catggggatg gcgggtgaat acggttccag cgccacctat gtcattgaaa gctggccaaa
     2701 acatctgcgt aacaaagcca gtggttttct gatttcaggc ttctctgtgg gggccgtcgt
     2761 tgccgctcag gtctatagcc tggtggttcc ggtctggggc tggcgtgcgc tgttctttat
     2821 cggcattttg cctatcatct ttgctctctg gctgcgtaaa aacatcccgg aagcggaaga
     2881 ctggaaagag aaacatgcag gtaaagcacc agtacgcacg atggtatata ttctctaccg
     2941 tggtgaacat cgcattgcca atatcgtaat gacactggcg gcggctactg cgctgtggtt
     3001 ttgcttcgcc ggtaacctgc aaaatgccgc gatcgtcgct gttcttgggc tgttatgcgc
     3061 cgcaatcttt atcagcttta tggtgcaaag tacgggtaaa cgctggccaa cgggcgtaat
     3121 gctgatggtg gtcgtgttgt ttgctttcct ctactcatgg ccgattcagg cgctgctgcc
     3181 aacgtatctg aaaaccgatc tggcttataa cccgcatact gtcgccaatg tgctgttctt
     3241 tagcggcttt ggggctgcgg tggggtgctg tgtcggcggc ttcctcggtg actggctggg
     3301 aacccgcaaa gcctacgttt gtagcctgtt ggcctcgcag ctgttgatta ttccggtatt
     3361 tgcgattggc ggcgcaaacg tctgggtgct cggtctgtta ctgttcttcc agcaaatgct
     3421 tggacaaggg atcgccggga tcttaccgaa actgattggc ggttatttcg ataccgacca
     3481 gcgtgcagcg ggcctgggct ttacctacaa cgttggcgca ttgggcggcg cactggcccc
     3541 aatcatcggc gcgttgatcg ctcaacgtct ggatctgggt actgcgctgg catcgctatc
     3601 gttcagtctg acatttgtgg tgatcctgct gattggtctg gatatgcctt cccgcgttca
     3661 gcgttggctg cgcccggaag cattgcgtac tcatgacgct atcgacggca aaccatttag
     3721 cggtgccgtg ccatttggca gcgccaaaaa cgatttagtc aaaaccaaaa gttaatcctg
     3781 ttgcccggtc tctgtaccgg gcctttcgct aagggaagat gtatgtcgtt acttgcacaa
     3841 ctggatcaaa aaatcgctgc taacggtggc ctgattgtct cctgccagcc ggttccggac
     3901 agcccgctcg ataaacccga aatcgtcgct gccatggcat tagcggcaga acaggcgggc
     3961 gcggttgcca ttcgcattga aggtgtggca aatctgcaag ccacgcgtgc ggtggtgagc
     4021 gtgccgatta ttggaattgt gaaacgcgat ctggaggatt ctccggtacg catcacggcc
     4081 tatattgaag atgttgatgc gctggcgcag gcgggcgcgg acattatcgc cattgacggc
     4141 accgaccgcc cgcgtccggt gcctgttgaa acgctgctgg cacgtattca ccatcacggt
     4201 ttactggcga tgaccgactg ctcaacgccg gaagacggcc tggcatgcca aaagctggga
     4261 gccgaaatta ttggcactac gctttctggc tataccacgc ctgaaacgcc agaagagccg
     4321 gatctggcgc tggtgaaaac gttgagcgac gccggatgtc gggtgattgc cgaagggcgt
     4381 tacaacacgc ctgctcaggc ggcagatgca atgcgccacg gcgcgtgggc ggtgacggtc
     4441 ggttctgcca ttacgcgtct tgaacacatt tgtcagtggt acaacacagc gatgaaaaag
     4501 gcggtgctat gaccacgttg gcgattgata tcggcggtac taaacttgcc gccgcgctga
     4561 ttggcgctga cgggcagatc cgcgatcgcc gagaacttcc gacaccagcc agccagactc
     4621 cgcaggcgtt gcgtgatgcc ttagccgcgt tagtcgcccc gttgcaagct catgcgcagc
     4681 aggttgccat cgcttcaacc ggaattattc gcgacggcag cttgctggcg ctgaatccgc
     4741 ataatcttgg tggattgcta cactttccgt tagtcaaaac gctggaacaa ctttccaatt
     4801 tgccgaccat tgccattaac gacgcgcagg ccgcagcatg ggcagagtat caggcgctgg
     4861 aaggcgatat aaccgatatg gtgtttatca ccgtttccac tggcgtcggc ggcggtgtag
     4921 tgagcggcgg taaactgctt accggccctg gcggtctggc ggggcacatc gggcatacgc
     4981 ttgccgatcc acacggccca gtctgcggct gtggacgcac aggttgcgtg gaagcgattg
     5041 cttctggtcg cggtattgca gcggcagcgc aaggtgaact catgggcgcg gatgcgagaa
     5101 ctattttcac acgcgccggg cagggcgacg agcaggcgca gcaactgatt caccgctccg
     5161 cacatgtgct ggcaaggctg atcgctgata ttaaagccac aactgattgc cagtgcgtgg
     5221 tggtcggtgg cagcgttggt ctggcagaag ggtatctggc gttggtggaa acgtatctgg
     5281 cgcaggagcc agcggcattt catgttgatt tactggcggc gcactaccgc cacgatgcag
     5341 gtttacttgg ggctgcgctg ttggcccagg gagaaaaatt atgatgatgg gtgaagtaca
     5401 gtcattaccg tctgctgggt tacatcctgc gttacaggac gcgttaacgc tggcattagc
     5461 tgccagaccg caagaaaaag cgccgggtcg ttacgaatta cagggcgaca atatctttat
     5521 gaatgtcatg acgtttaaca ctcaatcgcc cgtcgagaaa aaagcggaat tgcacgagca
     5581 atacattgat atccagctgt tattaaacgg tgaggaacgg attctgtttg gcatggcagg
     5641 cactgcgcgc cagtgtgaag agttccacca tgaggatgat tatcagcttt gcagcaccat
     5701 tgataacgag caagccatca tcttaaaacc gggaatgttc gccgtgttta tgccaagtga
     5761 accgcataaa ccaggatgcg ttgtcggcga gccgggtgag attaaaaagg tggtggtgaa
     5821 ggttaaggct gatttaatgg cttaaattta ttagatcagg tgtgacctga cccatctttg
     5881 gcacagagcc aaacctgaac tgacggaccg ctctgtgcca ttacctgacg ttgtattctt
     5941 ttatgttaac acccttgata ctgtatatac ctgtccgctt aattcgcttg ccctttttat
     6001 tcgtaatagt tgctgtaagt gtactgcagc gaatggaatg acgttcatca atcaccagcc
     6061 gaattgcttc tgaagcatct tccacctcta gaaggtcgtg agtcttattt aagccgaatg
     6121 gaacaagatg gaaggcagtt tttcctgatg attttctggt tatttttact ggatataaca
     6181 catcaccact tttatggatt aattcaagct gttcaatgta aatcatggca actttccttc
     6241 gtttatttgc atgaatatac aaaccgaaaa taggagatgc aatacaaaag ctgtggaaat
     6301 atcaaacagc ctctcaattt ggcagacagc gtctgccaaa tctttgccga tgggaattct
     6361 gcagatggtg tctgcggaat tcccaatggt gttaattagc cagacactgt ctggcgaatt
     6421 tcaacatgtt gaaaaaagag gtaaaaaagc ctcatttggc gtaagttttt tgcagagaac
     6481 ccacgcttat accttagact taaatccttt gatagtcgct tgatta
//
LOCUS       DAJTMK010000203         6353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTMK010000203 DAJTMK010000000
VERSION     DAJTMK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.207"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            291..1166
                     /locus_tag="OO135_003658"
     CDS             291..1166
                     /locus_tag="OO135_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR5399339.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1174..1950
                     /locus_tag="OO135_003659"
     CDS             1174..1950
                     /locus_tag="OO135_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR5399340.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2119..4380
                     /locus_tag="OO135_003660"
     CDS             2119..4380
                     /locus_tag="OO135_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5399341.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4449..5624
                     /gene="senB"
                     /locus_tag="OO135_003661"
     CDS             4449..5624
                     /gene="senB"
                     /locus_tag="OO135_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR5399342.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(5997..6185)
                     /locus_tag="OO135_003662"
                     /pseudo
     CDS             complement(5997..6185)
                     /locus_tag="OO135_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
BASE COUNT         1792 a         1482 c         1471 g         1608 t
ORIGIN      
        1 ttcgacatgt acatcaggcg cggaactcat cggggcgcgg gaatgaaatc ccattcagac
       61 tccggataga ttcaagcaag cccacttatc gtcaaaatcg gtgttgcaaa aacgggagtg
      121 accatagatc cgttttccaa gcggacccca taatatcggc tggtaaacat cactcgctag
      181 tgcattcttt ttgttggtaa tgataattat ttacatttat tctttgatgt tttatcataa
      241 ttaatgtttt atcaataaat atatggaaat atattgtttc agatgggaat atgagaaagt
      301 tcatccttat ttctatgata acgttagcta gtgttagtct gggagcctgc agacagaatg
      361 tcaccataaa gcaggatgcg ccagggcaga aagctttcct gattgacggg cagatttacg
      421 atttgcacag tggcaaaatt atttctagca gcgagctttt ggcggatctt gctaccgctc
      481 gacatcttat tattggtgaa aaacacgata atgccgagca tcatcagatt gagctttggc
      541 ttattcaaaa tctgctcatc cagcgaccac aggggagtgt gttgcttgag atgttgacgt
      601 cagaccaaca gccccgagtt aaccaagtta aatgttggct gaaagataat cctgtggttc
      661 gagatagccg tgttcaggaa ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg
      721 gcgacatcgt catgcagcta ttacgtgggc cttacccgct gctcaatgcc aacattggcc
      781 gggagcagat gttggcactg tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg
      841 cacctgtggt acaagaagct ctgcgtgaaa ccatcatagc tatgcatgaa ggcaaccttg
      901 aaagccagca actgacgtca atgctctctg ttcagcagca gcgggatcgc tacatggccc
      961 gccagcttct gagtgccccg gtaccgtcgt tgttgattgc aggtggttat cacgcgtcaa
     1021 aaagcatggg cgtaccgtta cacatggaag atcttgccac cggcacccat ccagtggtgt
     1081 tgatgcttgc agaaaaagga atgaacatca cagtagatca tgctgactac gtgtggtttg
     1141 tagcgccaga tacaacaaaa aggtaaacca tggatgatga atattctcca cttcccacag
     1201 tcggtaaaat ggagcagctg gtttatctgt tctctactgc ttcatggact gattttcctt
     1261 acgtttatct ggcgtttttc cgaagttcag cccgctatgt ctcctgcacc ggcgatcatg
     1321 ctgcaatggg cagaaattat agaggctcct tcttctccac tttcgcttcc ggtgggaatc
     1381 gctcaacagg agtccgcagt gacagaagaa aagcaacaaa ctgaggacag acaacaacgt
     1441 cctgtcactg aagacagtga tgcaaccatt gaaataacta ggaagaaaaa gtcatccgat
     1501 ggagaaaaga aaaagacacg tccacctcgt aagataaagg ctcaaaccag cgacagcaac
     1561 cccacagccg tttccagtaa tgctgcacct caggctctgg tcgaatcgtc ccgtattgct
     1621 gcgcctttta acagtgactc aacaaagcga gataattctg aggcatcttg ggaaagcaga
     1681 gtaaaaggcc accttaaccg ttataaacgt taccctggcg atgccagaaa acgtgcacga
     1741 accggcacag ccgttgtgac ttttaccgta aacacggaag gcactattgt cagtagtttc
     1801 ttggaaatct cttcaggaac cttttctctt gaccgcgaag ctattgctgt tttagaacgc
     1861 gctcaaccac ttcccaaacc accgcctgaa atattggagg gaggattatt taaagtaaaa
     1921 atgccgatta cctttaagtt gaaggagtaa agggataatt ggccgttcaa gttcatttaa
     1981 ctgaaattta attttcattt ttatgctgaa cattttatca aacttttcca ctgtcgaaaa
     2041 aataaaaaag acagtagatc ataattaata aacaaatagt tatacttatc tttattaata
     2101 ttatatccaa ggaaatttat gaacgttata aaactggcta tcggctcagg catattattg
     2161 ctcagctgcg gtgcttactc acaatccatc agtgaaaaaa ctaattccga caaaaaagga
     2221 gcggcagaat tcagtccgct cagcgtttct gtcgggaaga cgaccagtga gcaggaagct
     2281 ctcgagaaaa caggcgcgac cagttcccgg acaacggaca aaaacctgca atcacttgac
     2341 gcaacagtgc gtagtatgcc tggtacttat actcaaatag atcctggtca gggagcaatc
     2401 agtgtgaata ttcgaggcat gagcggattt ggtcgtgtaa acactatggt cgatggtatt
     2461 acccagagtt tttacggaac ctctacctcc ggaacaacgg cgcatggttc aactaacaat
     2521 atggctggcg tacttataga tcctaactta ctggtagcag ttgatgttac acgcggtgac
     2581 agcagtggct ctgaagggat caacgccctt gccggtagtg caaatatgcg tactattggc
     2641 gttgacgatg taatatttaa cggtaataca tatggccttc gttcacgttt ctctgtcggt
     2701 agtaatgggc tgggacgcag cggaatgatc gcccttggtg gaaaaagcga cgcttttacg
     2761 gatacgggaa gcattggcgt tatggctgct gtgagcggca gttctgtgta ctctaatttc
     2821 tcaaatggtt ctggaattaa cagcaaagag tttggttatg ataaatatat gaagcagaac
     2881 cccaaatccc aactgtataa aatggatatc agaccagacg aatttaacag cttcgaactt
     2941 tccgctcgaa cctatgaaaa taaatttaca cgtcgtgata taaccagtga cgactattac
     3001 attaaatatc attacacccc tttttctgaa ttaattgact ttaacgtaac ggccagtacc
     3061 agtcgcggta atcaaaagta tcgtgatggc tcgctgtata ctttctacaa aacctcagcg
     3121 caaaatcgtt ctgacgcgct ggatatcaac aataccagcc ggttcactgt cgcggacaat
     3181 gaactggagt ttatgctggg cagcaaactg atgcgtaccc gctatgaccg gaccattcac
     3241 tcagcggcgg gcgacccgaa agcgaatcag gaatcgatcg agaacaatcc gttcgcaccc
     3301 tccggccagc aggatatttc agcgctgtat accgggctga aggttacgcg cggcatctgg
     3361 gaggcagatt tcaatctcaa ctacacacgt aacaggatca cagggtacaa gcccgcctgc
     3421 gattcacgcg ttatctgcgt gccacagggt agctacgata ttgacgataa agagggtggc
     3481 ttcaaccctt cagttcagct ttctgctcag gtaacaccat ggcttcagcc gttcattggc
     3541 tacagcaaat ccatgcgcgc cccgaacatc caggagatgt tcttctctaa ttcaggaggc
     3601 gcatccatga acccattcct gaagcctgaa cgtgcagaaa cctggcaggc gggttttaac
     3661 attgatacca gagatttact ggtcgaacag gatgccctgc gctttaaggc tctggcgtac
     3721 cgcagcagga tccagaacta catctacagc gagtcttatc tggtttgttc tggaggtcgt
     3781 aaatgcagta tggctgaggt gattggcaat gactgggagg gcattagcga tgaatacagc
     3841 gacaatatgt acatctacgt taactcggca agcgacgtta ttgcaaaggg cttcgaactg
     3901 gagatggatt atgatgcagg ttttgctttt ggccgactct ctttcagcca gcagcaaaca
     3961 gaccagccaa cctccatcgc cagcacctac tttggcgcag gggatatgac cgaactgccc
     4021 agaaaataca tgacgctgga tactggtgtt cgcttcttcg ataacgcgtt gaccctgggc
     4081 actatcataa aatacacagg caaggctcgt cgcctgtcgc ctgattttga gcaggacgaa
     4141 cataccggcg caataatcaa acaggatttg ccgcagatcc caacgattat cgatctctat
     4201 ggtacttacg agtacaaccg caacctgaca ctgaaacttt cggtacaaaa cctgatgaac
     4261 agagattatt cggaggcgct gaataagctc aacatgatgc caggtcttgg tgacgagagc
     4321 cacccagcca attccgcgcg tggcagaaca tggatatttg gcggggacat tcgtttctga
     4381 tgacaatttc cgccaggttc agacaatacg ttttctctct tatgtcaatt ttattgcagg
     4441 aacgaaaaat gaatattttc actttatcca aagcaccgct atacttgtta atttcactat
     4501 ttttacccac gatggccatg gctatcgatc cacctgaacg cgaactttcg cgatttgccc
     4561 tgaaaacgaa ttaccttcag tcccctgatg aaggcgtcta tgaactggcg tttgataatg
     4621 ccagtaaaaa ggtgtttgca gcagtcaccg atcgtgtaaa tcgtgaagcc aataaaggct
     4681 atctgtattc gtttaattca gattcgctga aagtcgaaaa taaatacacg atgccatacc
     4741 gggcattttc gctggcgata aatcaggata aacatcagct ctatatcgga cacacccagt
     4801 cagcgtccct gcgtatcagt atgtttgaca ccccaaccgg taaactggta agaaccagcg
     4861 acaggttaag ttttaaagcg gcaaacgctg cagattcgcg ttttgagcat ttgcgccata
     4921 tggtttacag ccaggattcc gataccctgt ttgtgagtta tagcaatatg ctgaaaacgg
     4981 ccgagggcat gaagcctctg cataagctgt taatgctcga cgggacgacg cttgccttaa
     5041 aaggcgaggt taaggatgct tacaaaggta cagcgtatgg tctgacgatg gatgaaaaaa
     5101 cacagaaaat ctacgttggc ggaagagatt acatcaacga aattgatgcg aaaaatcaga
     5161 cgctgctgcg taccatcccg ttgaaagatc cgagaccaca aatcacaagt gtgcagaatc
     5221 tggcggtgga ctccgcttct gaccgtgcct ttgtggtggt attcgaccat gacgatcgtt
     5281 ccggtacaaa agatggcctc tatatttttg acttacgcga cggtaaacag cttggctatg
     5341 tgcacacagg agccggagct aacgcggtga aatacaatcc gaaatataac gaactgtatg
     5401 tcaccaactt tactagcggc accatcagcg tagtggatgc caccaaatac agcatcaccc
     5461 gtgaatttaa catgccggtc tacccaaacc agatggtgtt gtcggacgat atggataccc
     5521 tttacattgg catcaaagaa ggctttaacc gcgattggga tcctgatgtg tttgtggaag
     5581 gagctaaaga acgtattctg agcattgatt tgaaaaagtc gtgatcgggg gaaaaaacat
     5641 gggataaaaa ctccgttatt ccactaaact ggccggacta ggaaaagcaa acatctctgt
     5701 cctgccggta cgcctgttac gccgctccat gaggctgagg aatctccaga aaacgagaca
     5761 ggcacagcat tgtacgatca actgatttgt gccaataatt caatgaaatc acagctatgc
     5821 ctgttcacgt ataggaaatt gaaaaactaa gcgataccac tgtccgcgtc ttatgcctcg
     5881 tgcggtaagc tgtttttcac atcacgccgg tgtttttttc tttctgtgga cggttttaat
     5941 actgtaatcg gccgtttttt atacttaccc cgattcccgg gcgcacttat cctgcatcac
     6001 cccgggatat acctcagcag actgatatcc cgcgcgtttt tcttcagccc ctccacattc
     6061 agtcccgcga tggcctggcg gtataccatc cggccgccac acagcacgca ttcgaacggg
     6121 tcacgactca ggaactgttt caccatttgt gcatagcaca ctttcgccac tggttccggt
     6181 ttatcctgtt tttataacgg taattgcaga ccatgacaaa ccgcagccgt caggctgcct
     6241 actcgagtca caaggctctc tgtgtccgat atgccggcag cgcattaccc atgaaactgg
     6301 ctggaacgta caccataagg tgaagaaagt tatggggggc ggtgaagaat tat
//
LOCUS       DAJTMK010000204         6343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTMK010000204 DAJTMK010000000
VERSION     DAJTMK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.208"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..525)
                     /gene="yneK"
                     /locus_tag="OO135_003663"
                     /pseudo
     CDS             complement(1..525)
                     /gene="yneK"
                     /locus_tag="OO135_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(603..1484)
                     /locus_tag="OO135_003664"
     CDS             complement(603..1484)
                     /locus_tag="OO135_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5399343.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1585..2973
                     /gene="sad"
                     /locus_tag="OO135_003665"
     CDS             1585..2973
                     /gene="sad"
                     /locus_tag="OO135_003665"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR5399344.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            3037..3963
                     /gene="glsB"
                     /locus_tag="OO135_003666"
     CDS             3037..3963
                     /gene="glsB"
                     /locus_tag="OO135_003666"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5399345.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3963..4322
                     /locus_tag="OO135_003667"
     CDS             3963..4322
                     /locus_tag="OO135_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5399346.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4461..5879
                     /locus_tag="OO135_003668"
     CDS             4461..5879
                     /locus_tag="OO135_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5399347.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            6106..>6343
                     /locus_tag="OO135_003669"
     CDS             6106..>6343
                     /locus_tag="OO135_003669"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5399348.1"
                     /translation="MKTLNRRDIPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEK"
BASE COUNT         1528 a         1443 c         1684 g         1688 t
ORIGIN      
        1 gtggtgtatg cgcttttttg atggggacgc caaggtggat cattgtaaat tgttcaataa
       61 aatcattcag aaacagaaag agcacacgct cgacgccggt gtgaaatacg cgccccatag
      121 tgacaggctt tgggttggga gcgcaaaaat attaatttct ccatcatggc gaaaattatc
      181 taaataaata gggcttaaat ttattactac ctgaaattca atattttctt tgtgtcttcc
      241 attcttgacg ggataagtga gtttaaactg aaccttttca ttgtatagtg tgtcgttacc
      301 ccgaaaagaa gtcctctgaa tatatgataa gttatcaagg ttgttaaata tcttttttgt
      361 ggctgagttg gtgtttaaag acgtgcgcca ctcttcatca catattatgg tactgagtgc
      421 atcaataaaa ttctttaaaa cttcttttac ctgaggtgca atattacgta cgggaaaatc
      481 atcaggaaga gatatgtaat tactgatgct tactggcgta accatatttg ccccatatag
      541 tcataagtca tcggaacatc actatatagg gcatttgagc tgcaagaact atcaaaaata
      601 gctcattggt aaccctgttt tgccgagtca ggcacatcca gtagttgaat aaatgcctca
      661 agcggacgcg ttttcgcacc acgacgccag accagccagg ttgttaacca acgccattgc
      721 tcagctaacg gccacgcttc aacctggtga tgccccggca tactttccag catagagcgt
      781 ggaataagcg caatgcctgc tcctgcgacc acacaggcca acatcccgtg atacgactcc
      841 atctcgtgaa tagttcccgg agcggcaccg tcagcatgaa accagctctc gaagtggcga
      901 cgatacgaac aattggcgcg gaaggcataa atgttactgc cgttaacctg actggcgcgg
      961 attactggcg catgaccttg tggcgtgacg atcatcagct cctcgcggta taccggtatc
     1021 ccgtcaatgg cagtatggtt aatgggccca tcaataaatg ccgcattcag ttttccctcc
     1081 agcactcctt ccagcatagt gccggaaggg ccggtggaaa gtgaaaactg aattttggga
     1141 taacgatggt tgaattccgc cagcgtggcc ggaatgcgca ctgctgcggt gctttccagc
     1201 gaaccaagag aaaataaacc ttgcggctca tcgccagcga caacgctccg cgcttcatcc
     1261 actaacgcga gaatttgttg gctatagcgt aaaaagttat gcccggcagg agagagacgt
     1321 aaacgctgat tctcgcgaat aaacagatct acccccagtt ctgtttccag ctggcgtaga
     1381 cgggtagtga gattcgacgg cacgcgaagc actattgctg ctgcctgggt tatgctgcca
     1441 gcttcggcaa ccgcgttgaa catctccagt tgggtcagat ccataatatt ctcgattcgt
     1501 gaataagtgg cttaatatta ttcattttaa agcaagagta aatctgcgta tcttcatacc
     1561 atgactcata aaggagatac cccgatgacc attactccgg caactcatgc aatttcgata
     1621 aatcctgcca cgggtgaaca actttctgtg ctgccgtggg ctggcgctaa cgatatcgaa
     1681 aacgcacttc agctggcggc agcaggcttt cgcgactggc gcgagacaaa tatagattat
     1741 cgtgctgaaa aactgcgtgg tatcggtaag gctctgcgcg cccgtagcga agaaatggcg
     1801 caaatgatca cccgtgaaat gggcaaacca atcaatcagg cgcgcgctga agtggcgaaa
     1861 tcggcgaatt tgtgtgactg gtatgcagaa catggtccgg caatgctgaa ggcggaacct
     1921 acgctggtgg aaaatcagca ggcagttatt gagtatcgac cgttggggac gattctggcg
     1981 attatgccgt ggaactttcc gttatggcag gtgatgcgtg gcgcggttcc tatcattctt
     2041 gcaggtaacg gctacttact taaacatgcg ccgaatgtga tgggctgtgc tcagctcatt
     2101 gcccaggtgt ttaaagatgc gggaatcccg caaggcgtat atggctggct gaatgccgac
     2161 aacgacggtg tcagtcaaat gattaaagat tcgcgcattg ctgctgtcac ggtgaccgga
     2221 agtgttcgtg cgggagcggc tattggcgca caggctggag cggcactgaa aaaatgcgta
     2281 ctggaactgg gcggttcgga tccatttatt gtgcttaacg atgccgatct ggaactggcg
     2341 gttaaagcgg cggtagccgg acgttatcag aataccggac aggtttgtgc agcggcaaaa
     2401 cgctttatta tcgaagaggg aattgcttct gcatttaccg aacgttttgt ggcagctgcg
     2461 gcagccttga aaatgggcga tccccgtgat gaagagaacg ctctcggacc aatggctcgt
     2521 tttgatttac gtgatgagct gcatcatcag gtggagaaaa ccctggcgca gggtgcgcgt
     2581 ttgttactgg gcggggaaaa gatggctggg gcaggtaact actatccgcc aacggttctg
     2641 gcgaatgtta ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc tgttgcggca
     2701 atcaccgttg cgaaagatgc agaacatgcg ctggaactgg ctaatgatag tgagttcggc
     2761 ctttcagcga ccatttttac caccgacgaa acacaggcca gacagatggc ggcacgtctg
     2821 gaatgcggtg gggtgtttat caatggttat tgtgccagcg acgcgcgagt ggcctttggt
     2881 ggcgtgaaaa agagtggctt tggtcgtgag ctttcccatt tcggcttaca cgaattctgt
     2941 aatatccaga cggtgtggaa agaccggatc tgacgcgaca tattcagctc tgatatactc
     3001 gcaggtcttt tcagacctgc ggtccaggag tagaaagtgg cagtcgccat ggataatgca
     3061 attttagaaa acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa agtcgcggat
     3121 tatattccgg cgctggctac agtagacggt tcccgattgg gcattgctat ctgtaccgtt
     3181 gacggacagc tttttcaggc cggagacgcg caagaacgtt tttccattca gtctatttcc
     3241 aaagtgctga gtctcgttgt cgccatgcgt cattactccg aagaggaaat ctggcaacgc
     3301 gtcggcaaag atccgtctgg atcaccgttc aattctttag tgcaactgga aatggagcag
     3361 ggtattccgc gtaatccgtt tattaatgct ggtgcgctgg tggtctgcga tatgttgcaa
     3421 gggcgattaa gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt aagcggtgtg
     3481 tctgatattt catacgatac ggtggtagcg cgttccgaat ttgaacattc cgcgcgaaat
     3541 gcggctatcg cctggctgat gaagtcgttt ggcaatttcc atcatgacgt gacaaccgtt
     3601 ctgcaaaact actttcatta ctgcgctctg aaaatgagct gtgtagagct ggcccggacg
     3661 tttgtctttc tggctaatca ggggaaagct attcatattg atgaacctgt ggtgacgcca
     3721 atgcaggcgc ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca gaacgcgggg
     3781 gagtttgcct ggcgggtggg gctaccggcg aaatctggcg ttggtggcgg tattgtggcg
     3841 attgttccgc atgaaatggc catcgctgtc tggagtccgg aactggatga tgcaggtaac
     3901 tcgcttgcgg gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg ttcggtttat
     3961 taatgcagtc tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt cgctctcgct
     4021 ttcgtctggg catgaaagag cgtcagtatt gcctggagaa aggcgcgcca gtcatcgaac
     4081 aacatgcggc ggattttgtc gctaaacgtc ttgctccggc attaccggct aatgacggta
     4141 agcaaacacc catgcgcggg catccggtgt ttatcgctca gcacgccacc gcgacctgct
     4201 gtcgcggttg tctggctaag tggcataaca ttccgcaggg tgtttcgtta agcgaagaac
     4261 aacaacgtta tatcgtggcg gtaatctatc actggctggt tgtacagatg aatcagccat
     4321 gagaattgcg gaaaacgata aaatagcatt cccagggtac ctttatttgc cgccgctgtt
     4381 agcgaacatg ctatccttta tgagaattta cttattacgt tgagataatt gccacccgca
     4441 attgtatgga cagataatta atgcatgtac accccatttc aactttccgt ttgttccagg
     4501 aaggtcatct gctacgtaat agcatcgcta tttttgcgct aaccacgctg ttctatttta
     4561 ttgttgcaga gttacggctg gttcacgaac tttctctttt ctggccgctg aatggcgtaa
     4621 tggcgggggt gtttgcccgc tatgtctggc ttaatcgact gcattactat gcgatcagtt
     4681 atgtggcgat gctggtttat gatgccataa ccaccgaatg gggactggtt tcactggtta
     4741 tcaatttctc caatatgatg tttattgtta ccgtcgcctt actggtcgtg cgggataagc
     4801 gtctggggaa aaataagtat gagccagtaa gtgccttacg gctatttaat tactgtctga
     4861 ttgccgcatt attatgcgct attgtcgggg cgattggttc ggtcagtatt gatagtctgg
     4921 atttctggcc tttgcttgcg gactggttca gtgagcaatt ctcaacgggc gtgttgatcg
     4981 tgccttgtat gctgacgttg gctattcctg gagtgctgcc gcgctttaaa gcagaacaga
     5041 tgatgcccgc catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt ggcggagcgg
     5101 ggagtctggc gttcccgctg cctgcattaa tctggtgtgc agtgcgctat acgccgcagg
     5161 taacatgtct gttgaccttt gtcaccggtg cggtggaaat cgtactggtg gcaaattcgg
     5221 tgattgatat ctcggtcggt tcgccgttct ccattccgga aatgttctcc gcacgtctcg
     5281 gtattgccac gatggcgata tgcccaatta tggtttcttt tagcgtggca gcgatcaatt
     5341 cgctaatgaa gcaagttgcg ctgcgagccg actttgattt tctgactcag gtttactcac
     5401 ggtccggtct ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa catctgacgg
     5461 tcatgctgct tgatatcgac tatttcaaaa gcattaacga taactatgga catgaatgtg
     5521 gcgataaagt gttaagcgtg tttgcccggc atattcagaa gattgtcggt gataaggggc
     5581 tggtggcgcg catgggcggc gaagaatttg ctgttgcagt gccgtcggtg aatcctgtcg
     5641 atggtctgct aatggcggaa aaaatccgta aaggcgttga actgcaacca ttcacctggc
     5701 aacaaaaaac gctttatctc acggtaagta ttggcgtcgg tagtggtcgc gcatcgtacc
     5761 gaacgctgac cgatgatttt aataaattga tggtcgaagc cgatacgtgt ctgtatcgct
     5821 cgaagaaaga tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa gttgtctgac
     5881 atctttattc attccgcgcg atttgaatgc aatgtgttct accactttat gatccaggaa
     5941 aaggcgtatt tcctcgatta aaccatgatc cgcgccacac ttttctcata aaactgctca
     6001 ttaatcatca ggagttgatc aaaacagggg gtcggcgtat taaagtttac taacatgtcg
     6061 taccaattta aacggcttgt atgactaatc agaagggaac ccattgtgaa aacactaaat
     6121 cgtcgcgata ttcccggtgc acagtatcca gaacgtatca ttcagtttgg tgaaggtaac
     6181 ttcctgcgcg cctttgttga ctggcaaatc gatctcctga atgagcacac cgatctgaat
     6241 tctggcgtgg tcgttgttcg tccgattgaa acatcattcc cgccgtcact gagcacgcag
     6301 gatggtctgt acaccaccat tatccgtggc ctgaatgaga agg
//
LOCUS       DAJTMK010000205         6265 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTMK010000205 DAJTMK010000000
VERSION     DAJTMK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.209"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="OO135_003670"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="OO135_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5399349.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="OO135_003671"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="OO135_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR5399350.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            835..1995
                     /gene="pheA"
                     /locus_tag="OO135_003672"
     CDS             835..1995
                     /gene="pheA"
                     /locus_tag="OO135_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5399351.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="OO135_003673"
     CDS             complement(2038..3159)
                     /gene="tyrA"
                     /locus_tag="OO135_003673"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5399352.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="OO135_003674"
     CDS             complement(3170..4240)
                     /gene="aroF"
                     /locus_tag="OO135_003674"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5399353.1"
                     /translation="MQKDAQNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="OO135_003675"
     CDS             4448..4813
                     /locus_tag="OO135_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5399354.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="OO135_003676"
                     /pseudo
     CDS             4963..5481
                     /locus_tag="OO135_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
     gene            5471..6265
                     /gene="dgcN"
                     /locus_tag="OO135_003677"
                     /pseudo
     CDS             5471..6265
                     /gene="dgcN"
                     /locus_tag="OO135_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
BASE COUNT         1699 a         1598 c         1411 g         1557 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat tcccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacactgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaaccgggg ggcctttttt attgataaca aaaaaggcaa cactatgaca
      841 tcggaaaatc cattactggc gctgcgagag aaaatcagcg cgctggatga aaaattatta
      901 gcattactgg cagagcggcg cgaactggcc gtcgaggtgg gaaaagccaa actgctctcg
      961 catcgcccgg tacgagatat tgatcgtgaa cgcgatttac tggaaagatt aattacgctc
     1021 ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct catcattgaa
     1081 gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat taatccgcac
     1141 tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc ggcgcgccag
     1201 tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt tgccgatatt
     1261 tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga aaataccagc
     1321 tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagcttgtc gattgttggc
     1381 gagatgacgt taactatcga ccattgtttg ttggtctccg gcacgactga tttatccacc
     1441 attaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt ccttaatcgt
     1501 tatcctcact ggaagattga atataccgaa agtacgtctg cggcaatgga aaaggttgca
     1561 caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac tttgtacggt
     1621 ttgcaggtac tggagcgtat tgaagcgaat cagcaacaaa acttcacccg atttgtggtg
     1681 ttggcacgta aagccattaa cgtgtctgac caggttccgg cgaaaacgac gttgttaatg
     1741 gcgaccgggc aacaagccgg tgcgctggtt gaagcgttgc tggtgctgcg caaccacaat
     1801 ctgattatga cccgtctgga atcacgcccg attcacggta atccatggga agagatgttt
     1861 tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt gaaagagtta
     1921 ggggaaatta cccgttcaat gaaggtattg ggctgttacc caagtgagaa cgtagtgcct
     1981 gttgatccaa cctgatgaaa aggtgccgga tgatgtgaat catccggcac tggattatta
     2041 ctggcggttg tcattcgcct gacgcaataa cacgcggctt tcactctgaa aacgctgtgc
     2101 gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct gcttatcgcc
     2161 ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga ttaacgccag
     2221 attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag caaacagtcg
     2281 cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagaa gttgctcaag
     2341 ctgaacattt tcttctgcca gatgcagccc ataagcaaaa gtagcaaagt ggcgcagagc
     2401 ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca gccgagcgcc
     2461 ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc catcacacca
     2521 gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt gtaaccccag
     2581 taccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca ctgatgccag
     2641 atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt gctcagtaac
     2701 gtggattggc acactaacaa tcaccattcc ggcatcggca acaatatcag ccgctcgatc
     2761 ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtgtcagca tcttctcgaa
     2821 caggcgtccc atctgaccgc caccgccgac gataaccacc ggacgcagtg acggacaaag
     2881 tgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc gacgcaaaac
     2941 atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac gcgaggccaa
     3001 catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt tcacctcgcc
     3061 cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt tatcgacttc
     3121 atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct taagccacgc
     3181 gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca tcggtcattt
     3241 cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg cgcggttgct
     3301 cagaagactg attgccctcg tggatattac tttcgatcat cagaccaata attgagcgat
     3361 tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg cgataatctt
     3421 tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc gcctgttcca
     3481 tctctttttc acattgcgca acatccgcag ggctatagtt cggcgcttta ccaccgcgca
     3541 ggatgacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc cctgcctggt
     3601 taatgccaac aaaacggtgc ggctgggcgg cggcgcgcat agcgttaatt gctgttgcca
     3661 gactgccgtc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag gccatttcac
     3721 ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac agatcgccca
     3781 ggtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc atattcacca
     3841 gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa gagccatcca
     3901 tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt tcaaaataga
     3961 cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta aatcgacgag
     4021 catattccag agcagtttcc ggatcatgaa tggaacaagg accacaaact accagcagac
     4081 gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca atctgggctt
     4141 cttgttgcag gctcaatgga aaagcggcct tcagttgttc cggagtcatt aaaacctgtt
     4201 cgtcggtaat atgtacgtta ttctgcgcgt ctttttgcat gatggcgatc ctgtttatgc
     4261 tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata aagtaaagtt
     4321 ttcaatccat atttcgtacg cttttcttta cacgaacaat ttactcgcgc tttgatgctt
     4381 atttcgtaca aataaattta cacctccttt aaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttattggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttggcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgta acaatgttga
     4741 aagtgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac gatcgcatga aaaaatgata ttttaatgag cattctgaaa
     4921 aaatatcgat gcatttcgag cgaagaaggt ggggatccct gaatgcgttt ttctcactga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca ggagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgctgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcaattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcgc ctggcattac acccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccccgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caatcagcca ttttatctgg
     5941 ttttcgctcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcgctgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttctg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgcgcgc
     6241 agcgtttcgt agcggcatca atacg
//
LOCUS       DAJTMK010000206         6230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTMK010000206 DAJTMK010000000
VERSION     DAJTMK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.210"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(112..1503)
                     /gene="tcyP"
                     /locus_tag="OO135_003678"
     CDS             complement(112..1503)
                     /gene="tcyP"
                     /locus_tag="OO135_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5399355.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLVLMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLTAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1636..2226)
                     /locus_tag="OO135_003679"
     CDS             complement(1636..2226)
                     /locus_tag="OO135_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5399356.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            complement(2389..3057)
                     /gene="hxpB"
                     /locus_tag="OO135_003680"
     CDS             complement(2389..3057)
                     /gene="hxpB"
                     /locus_tag="OO135_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5399357.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3204..3740
                     /locus_tag="OO135_003681"
     CDS             3204..3740
                     /locus_tag="OO135_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5399358.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3781..4641)
                     /locus_tag="OO135_003682"
     CDS             complement(3781..4641)
                     /locus_tag="OO135_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5399359.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4747..5037)
                     /gene="ghoS"
                     /locus_tag="OO135_003683"
     CDS             complement(4747..5037)
                     /gene="ghoS"
                     /locus_tag="OO135_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5399360.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5138..6067)
                     /gene="pfkB"
                     /locus_tag="OO135_003684"
     CDS             complement(5138..6067)
                     /gene="pfkB"
                     /locus_tag="OO135_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5399361.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1622 a         1557 c         1522 g         1529 t
ORIGIN      
        1 tttttcgctg acgatctgtt taaatatgag tcagtatgca tcgccgcgat ggaacattta
       61 aaaagtaaag caacggcccc tatacaaaac ggaccgttgc cagcataaga attaacggtg
      121 tgccagttcg gcgtcgtctt cgctatccag aatggcttta tcggtttgct tcagccactg
      181 gctggtcagc gtgccagctg tcatcgagcc actaacgttt agcgccgtac ggcccatgtc
      241 gataagcggt tcaacggaga ttaacagcgc caccagggtt actggcaggc ccatcgcagt
      301 cagcacaatc agtgcggcga aagttgcacc accaccgaca ccggcaacgc ctgcggaact
      361 aacggtaaca ataccgacca gcgtcgcaat ccacatcggg tccagcgggt taatgccaac
      421 cgtaggcgca accatcaccg ccagcattgc cggatacaaa ccggcgcagc cgttctgacc
      481 aatggttgca ccgaaagagg cggcgaaact ggcgatggat tcaggaacgc ccagacgacg
      541 cgtttgtgct tccacattca gtgggataga cgcagcactg gaacggctgg taaaggcaaa
      601 cgtcagcaca ggccatacct tacggaagta cttcagcgga ctcacgccat taatgcccag
      661 cagaatgcca tgcactgcaa acataatcag cagaccgagg taggacgcga caacgaaact
      721 tcccagtttg atgatgtctt gcaagttaga acctgcaacc actttggtca ttagaaccag
      781 aacgccgtaa ggggtcaact gcatgacaag gcgaaccagt ttcatcaccc agctttgtag
      841 ggtatcgata gcggctaaga cgcgttcacc tttcggcgca tcatccttca gcagtttcaa
      901 tgcagctacg ccgaggaatg cggcaaaaat taccacgctg ataattgacg tcggattggc
      961 tccggtaaga tcggcaaacg ggtttttcgg gataaaggac aagaccagct gcggaacgct
     1021 cagatcagag actttaccaa catagttact ttcaatggcg ttcagacgtg cagtttctgc
     1081 accaccctga accagacctt cagccgtcaa accaaacagg ttggtgacca gcacaccgac
     1141 cagcgccgca atcagcgtgg taaacaaaag cgtaccgatg gtcagaaaac tgattttgcc
     1201 taactgagat gcgttatgca gacgggcaac cgcgctcaga atagaggcga agactaacgg
     1261 cataacgatc atttgcagca gttgaacata gccgttacca acgatgttaa accactgtac
     1321 agaatcttta agtacctggc tgtcagaacc ataaatggta tgcagggcaa ggccaaaaac
     1381 cacacccatc accagaccca ccagcacttt tttcgccaga ctccactgtt tatggcgggt
     1441 ctgagccagc gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt
     1501 catccccgtt ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc
     1561 aagattaaca gaactgtgag ctggctctta tattcaaatg gaattgctta ttccagaaag
     1621 gtgatttttg tctttttagt attcaacctg atgcttaata agccgatgaa actggatctg
     1681 caaggtattg attatttgcg atgaccaacg aacagcgctg ttctcgggta atgaatgggg
     1741 catccatacc gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttgccctt
     1801 ttggggaact aggataggca agcggaaacg ccagcggcat ggccagagca ggggaacacc
     1861 ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa cccagcacca ttccttgtag
     1921 cgcatcagcc ggaatgaacc agccttccgg aaccttaagg taaaaggttg ccagcagcgc
     1981 aaataccgcc agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgatcggttt
     2041 tgatatccat tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa
     2101 acacgtcagg attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt
     2161 cagctcggca tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc
     2221 cgtcatgata aaactcacta aactgtcgat acgtacagta taggattttt atacagtgca
     2281 cggaagtggt gacggtgtaa ctctttctct taaattgacg attaatccgt cagttgaatg
     2341 caaattcaga taaagaacca ggggcagcgt gatcactgcc cctggtcatt aaccgagaag
     2401 gtcttttgcg gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg
     2461 atcattttgc gcttctggcg caggaacgac gatggaacgc atgcgggctg ctttagaagc
     2521 gatcatgcca tttaccgagt cttccagcgc cacgcaggtc agtgggtcaa cgcccagttt
     2581 tgctgcgcag tcgagatata cttgcggatg cggcttgctg taaggcagtt tttcagccga
     2641 ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt ccagcatatg
     2701 tagtggtgac gcggaggcca gtcccaccaa taaaccttgt tctttgcata acgcaacagc
     2761 ttcgcgtacg cctggtaata atggacgtgt ctcttcaacc agtgaaatgg cacgggcaat
     2821 aacccgttct actacttcct gacggcttgg cccattccac ggttgccggg cgtaccaaag
     2881 atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc agctcgttac gacgggagat
     2941 atccaccccc aggcttgcca tcacatccag ttcggctcga tcccataaag gttctgagtc
     3001 gataagtaat ccatccatat caaaaattgc agcaagaatc tgacgcgggg ttgacattac
     3061 aacctctcct ttagagcatt atgtgtaagg tattaagccg tgcttatgat taagcgccta
     3121 tcatatcgcc tttaaagaat gggcgaaaac agatatctac aggtaaacta agtgaaacga
     3181 cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt gctgttttga
     3241 aaaggatttt gggttgggtg atttttatac ctgcattgat ctctacattg atttctttgc
     3301 tgaaatttat gaatacccgg caggaaaacc aggaaggcat taatgcggtc atgctcgact
     3361 ttactcatgt catgatcgat atgatgcagg cgaatacgcc ttttttgaat ctgttctggt
     3421 ataactcccc gacgcccaat tttaatggtg gcgtgaacgt tatgttctgg gtgattttca
     3481 tcctgatttt tgtcggactg gcgttgcagg attccggtgc ccgaatgagt cgccaggcgc
     3541 gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa ggggaagagg
     3601 ggctaacgcg tgaacaaatt gagtcccgta ttgttgttcc gcaccatact attttcctgc
     3661 agtttttctc actgtatatc ctgccggtta tttgtattgc tgcaggttat gtgttctttt
     3721 ctctgcttgg gtttatttaa tcaaaaggcc gcacagagcg gccattatct tcaacccata
     3781 tcatgctgct aataatctat ccagtgactg ctgagcaata accaaatgct gaccgccaaa
     3841 taaccttgca cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt caaggaaatc
     3901 ggcaggtagc ggggatactg actgatagcc gtcatagatt tgtggcggct gttcagtatg
     3961 cagcggtaac atcgccaggt cgcactctcg gtcaccccag tagcaggcag ggtcgaaaat
     4021 gtacgggcca tccggaccca gcgcacagtt gccggaccat aaatcgccgt gcaacagaga
     4081 aggctgcggt tgatgcgagg ccagacgctg ctgaatatgc tcgacgatgg cgtcgatatt
     4141 gccgaaagcg atccctttct ctgctgccag ttccaactgc cagccaatcc gttgttcagc
     4201 aaaaaacgtt gaccagcgac gttgccaggt gttgggctgt ggagttgtgg agagcgagtt
     4261 atcaaaatcg aggccaaatt gtggttggtc actccattga tgtaaacgcg caatttgctg
     4321 accaagaata aatgcgctat gcgcatccag cggacgaggt gggagataat ccatcaccag
     4381 aaaactgtag tcacggtcag cgcctactgc ccaaacctta ggcacggtga cggttttact
     4441 acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca gttccctttc
     4501 atcacatttg acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg catggacttc
     4561 tccgccaggc agttcattac gcagttcgat ttcgccttca cctaactgct cgctcaaaag
     4621 acgactgatt gcctgccaca tgatgtctct cccatgttgt ctgccagatc ataaggttag
     4681 cgcaagaatg cggtgtaaaa acacgagcta acgcacacca cagactgatt attgcccaaa
     4741 aaccatttat tttctgttgc tattccattc ctcccaggtc gtgatggtga tttcaggatc
     4801 tttgccggtt gcactttggg taagccccga aaccagttct ctaatttctt cttcactttg
     4861 ggtactgata agtccaaaag tgttagtccc cagctcatgg atatttccct catcatccgt
     4921 catggtgagc aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa tttcagtcaa
     4981 tgtatcctca tgcaacatga cggttatgac ataacggaca agatcgccac tggccatagt
     5041 tcaccccttt gttatcacat gacttttgag catagtcgga gaaacgcgtt gccgacaggt
     5101 tggtggtgat tcccccaatg ctgggggaat gtttttgtta gcgggaaagg taagcgtaaa
     5161 ttttttgcgt atcgtcatgg gagcacagac gtgttccctg attgagtgtg gctgcactcc
     5221 ccgcagctac gccaaaacgg accatctctt caagagaggc attttctgcc agtttcagtg
     5281 tcatcgcgcc gaccatgctg tcaccagcgc caacggtact ctggcttttc accggtggtg
     5341 gcaccacctg aatgcagttt tcactatcaa cacccagcgc tccttgtgga ccaagggaaa
     5401 cgacaacccg tttggccttg ccgctattaa cgatttcctg cgcggctttg cggacatcgt
     5461 ccggctgggt gagttcgcga ttcaccagcg cactgagttc tttttggtta ggcttaacca
     5521 actcgatgtt accaattgcc agtgctgcac ttaacgcttc gccagaactg tcgacgatgc
     5581 agcggatccc ttgtttttgc gaagcggaaa tcagttgggt taatttttcc agcttcacac
     5641 ctggcggcag gcttccgctt atgactagga tggccccgga ttcaatttcc agaacttgct
     5701 cttcaagctg gcgaaactca tcttcattta atgccgcgcc tggcataaca aaacgatact
     5761 gctcaccgct tgcttccaca tgtacatgta aattctgccg ggtccagtct ttggcttcta
     5821 cagtagcgac ggggacattt tcatccgcca acagtgaaac caggtgttcg ccggtcgcgc
     5881 cacccgccgg gaagatcgct gtggcactgc ctccaagatg ggcaatggcg cgggcgacgt
     5941 tgatgccgcc gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt ccttcgggat
     6001 aaatttgcgg ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac gtatagatac
     6061 gtaccatcat ttcctcctat aggctgattt cagtctggca ccgaatcagc ggaaagtgaa
     6121 gaaattaaca atatgattta ttggagcttt atttaaaatt ttgcagataa atatatataa
     6181 ataaaaatct gttttcgcca catgcttatt caaaaaatga aataagaata
//
LOCUS       DAJTMK010000207         6224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTMK010000207 DAJTMK010000000
VERSION     DAJTMK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.211"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(151..801)
                     /gene="zinT"
                     /locus_tag="OO135_003685"
     CDS             complement(151..801)
                     /gene="zinT"
                     /locus_tag="OO135_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5399362.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1058..1693)
                     /gene="msrQ"
                     /locus_tag="OO135_003686"
     CDS             complement(1058..1693)
                     /gene="msrQ"
                     /locus_tag="OO135_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5399363.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWALNHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1694..2698)
                     /gene="msrP"
                     /locus_tag="OO135_003687"
     CDS             complement(1694..2698)
                     /gene="msrP"
                     /locus_tag="OO135_003687"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR5399364.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGMKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2807..3220)
                     /gene="hiuH"
                     /locus_tag="OO135_003688"
     CDS             complement(2807..3220)
                     /gene="hiuH"
                     /locus_tag="OO135_003688"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5399365.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3353..4024
                     /gene="hprR"
                     /locus_tag="OO135_003689"
     CDS             3353..4024
                     /gene="hprR"
                     /locus_tag="OO135_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5399366.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4024..5382
                     /gene="hprS"
                     /locus_tag="OO135_003690"
     CDS             4024..5382
                     /gene="hprS"
                     /locus_tag="OO135_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5399367.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVTDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYINKHNVFRITLPQ
                     RN"
     gene            complement(5490..>6224)
                     /gene="hchA"
                     /locus_tag="OO135_003691"
     CDS             complement(5490..>6224)
                     /gene="hchA"
                     /locus_tag="OO135_003691"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5399368.1"
                     /translation="DYPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLY
                     HLHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLN
                     ADSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDN
                     PVNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKL
                     LTGDSPFAANALGKLAAQEMLAAYAG"
BASE COUNT         1725 a         1320 c         1448 g         1731 t
ORIGIN      
        1 aactcaatgc gggttagctt attaatatac agacacgtca tagtgatgag cgtgtctgta
       61 gctagcttgc gttcagtggc gtttgtggat ggaccagtgg cagttatgat gacttactaa
      121 agcggcatcg aggcgttatc atgagaatac tcaatgagac atcatttcct cgaccacttc
      181 ttcgctactc aactgatatg gataatacgt tggccagttt tccatttcat ttaataaaga
      241 ttgctgtgaa tcattaccca taaaaatgtg aaaatgagat gattttcgtg gtgcaattat
      301 atgatcgcta aattgtatat atttaggggc tttgctttca ggatctttac attcgaataa
      361 gtaacgaacg cctttcttgc ctgatttata ggtgagtatt ttgtatccat cgtaatcata
      421 tttacaggat gttgtttcat tatttctatg gaattcaaca atgccgtcct caatgccaat
      481 catctcgata tctgttgcat aacctttgtg ataataatct ttaatttcag caaatgtttt
      541 agttttatct gcatccgctt tcttctgaaa gacggggtca agtttgccac tttgcagtaa
      601 aggataaacg gattgccaga ctccatccca gtcactgagc gttcggtttt gtacattggc
      661 atcatcaaaa acaccattag cagctttttg ttcgacctct gttaagggtt tgccgtgtga
      721 gtgatgacca tgcgaaaagg caggagcgct aacaataaag acacctaacg caacagccag
      781 tttgtgaaga cgaatcgcca aaacagtttc ctccagagtc attgtatatg atgtgtaatg
      841 ttatattgta acatatggca aaatgcaaca actcttagga gatgagagca tagttttctc
      901 aggtgtactt atctatctta ttgaaataaa atgaattata ttgataattt gaggttgatg
      961 acaaacgaaa cataatctga tgtgatctgc gtaggccgga tagggcgctc gcgccgcatc
     1021 cggcagggat aacgcgtact ttgtcggcaa tatgaagtta tacagataat ttgttgtgaa
     1081 cctgcttgcg taaccggtta aaaagggaac gcaacttctt ataccgtaag gctaaaagca
     1141 gtacagccag cccagcgtag atgagcggct gcggtgaaat aatcttcact gaccacagat
     1201 aatgtattgg ggccaggatc gcgacaagat agacgaaatt atgcaactgt tgccagtgct
     1261 tgccgagttt tcgctgcatc gcctgggttg aagtaaacgc taaagcaagc aaaataaccc
     1321 agctgataat tcctaacgtt aaataaggcc gggtaattaa ctccttgcct aataacgcca
     1381 gattattcac gcccagctcc agcaatgcgt aactggttaa atgcagtgtc gcccaggcaa
     1441 agcaccataa gcctaacagg cggcgagtgc gtatcaataa cggctgtttt gcgtagcgtg
     1501 ccagaggtgt gattaacaag gtcgccagca aaaatttcag tgcagtgcga ccggtaaaat
     1561 gctgaatatc tttcaccgga tcggcaccca gtccaccgtg attgagcgcc cagaccagcc
     1621 agagaaacgg caacaatccg gcaagatgca ggctaacttt cagccatgtc acctgttttg
     1681 ctgtcagacg cattcagaaa ttctcccgca aatccaggcc acgatacagc gatgccacct
     1741 ggtcggcgta accattaaac agtagcgttg gctggcgctg aacatcgagg atgccgcctg
     1801 aaccaataaa tcgttcggta gcctgtgacc agcgcgggtg atcaacatgc ggattaacgt
     1861 tggcgtaaaa accgtattcg tcaggcgctg ccagattcca ggtggttggc ggacgctcgc
     1921 gggtcagctt aatactgact atcgatttaa tccctttaaa gccatatttc cacggcacaa
     1981 tcagtcgtac cggcgcgcca ttttgtggcg gtaacgcctt gccataaaca cccacggtca
     2041 tcagtgtgag cggatgcatt gcttcgtcga gacgcaatcc ttcgacataa ggatatttca
     2101 tcccgccgcc gataaagcgg tcctgctggc ctggcatttg ttccggtgca taaattgttt
     2161 cgaaagcgac atacttcgca ttgctggtgg gttcggcaag cgccagcaat ttgtgcagcg
     2221 gaaaaccaat ccacggcacc accatcgacc atgcctccac gcagcgcata cgataaatac
     2281 gctcttccag cgggaagcga cgggttaaat catcgtgatc gagggtcaat ggttttgcca
     2341 cttcgccgct gattttcagt gtccatggat cggttttcag gctaccagca ttagcggcgg
     2401 gatcggcttt atccagcccg aattcataga agttgttata accggagact ttatctactg
     2461 gcgtcagtgg caggttattt tgccaggcgg caggcttgct gaactccagc ggttttccgg
     2521 cgggggcggg cgggcgatcg ttccctttaa accagctaag cagatcggca tgcgcagcgt
     2581 gaggcaaaga aagtgcagct gcactgatgc ccagtgcttt taacacctgc cgacgcttca
     2641 taaagaatac cgactcggcc gtaacatctg attcttttaa aaattgattc tttttcataa
     2701 gccatcacac ttttttgtcg ttagccagca agagccgttt actgttaatc tgtcagggag
     2761 tgtcctccct aacagtagca gagatctgct tttacatatt tatgcattaa ctgccacgat
     2821 aggttgaata cccatattgg ctaagtaata aaggcacatg ataatgctcg ttcactttat
     2881 taatatgaaa ttcaacgggg atctcaggga agaaactttc aagattttgt ttcttgaaat
     2941 agtccccggt tttaaatacg acacggtaat cgcccgtagt tgcagtttgc tcgggccaca
     3001 gtgccttaat tcgtccatcc ttatctgttt tggcggtatt aagttgtaac cagccgttat
     3061 ccgccttctt ttcaagagtg actgtcacgt cagcagcggg ttttccggtt tgctggttca
     3121 aaatgtgcac gctaagaatg ttttgttgtg cggcataaac caaagaaggt aatgaaaatg
     3181 ctgccgttgc taccgagagt actaaataac gctttaacat gtttatatcc ttgtcatgtg
     3241 aatgagtttt gaggatagcg aaaaagcggc cagggtaacc ttacagcagc attacaattt
     3301 tgtaatgaac caggctgttt ctataacata tgatttatgg catattattt tcatgaagat
     3361 tttacttatt gaagataatc aaaggaccca ggagtgggta acgcaggggc tttccgaagc
     3421 gggttatgtc attgatgccg tttctgatgg cagagatggg ctttatcttg cgctgaagga
     3481 tgattatgca ttgataattc tggatattat gcttccgggt atggatggct ggcagatctt
     3541 acaaacgtta agaacagcaa agcaaacccc tgttatttgc cttactgcaa gggattctgt
     3601 cgatgacaga gtcagagggc tggacagtgg agcaaatgat tatctggtaa aacctttttc
     3661 attttctgag ttgctggcaa gggttcgggc acaattaagg caacatcacg ctttgaattc
     3721 aacattagaa atcagtggct taagaatgga ctctgtaagt caaagtgtga gcagggacaa
     3781 tatcagtatt acactgacgc gcaaggagtt tcagttactt tggctactgg cctccagagc
     3841 tggcgaaatt atacccagaa cggttattgc gagtgaaatt tggggaatca actttgatag
     3901 tgataccaat acggtggatg tcgccattcg caggctccgc gcaaaagttg atgatccttt
     3961 tcctgaaaag ctaatcgcca caatccgggg gatgggctat tcattcgtag cggtaaaaaa
     4021 ataatgaaaa gattgtctat aaccgtccgt ttaaccttgc tttttatatt gctgctgtct
     4081 gttgctggcg ctggaattgt ctggactctc tataatggcc tggcaagtga gttgaaatgg
     4141 cgcgatgata caacactcat taaccggaca gcacagatca agcagttgtt aattgatggg
     4201 gtaaatccag atacgttacc tgtatacttt aaccggatga tggatgttag tcaggatatc
     4261 ttgatcattc atggtgatgg catcaataaa attgttaacc ggacaaatgt cagtgatgac
     4321 atgttaaata acatacctgc tagtgagaca atcagcgcag ctggcattta cagaagcatt
     4381 attaatgata ctgagattga tgctttacga attaatattg atgaagtttc gccatcatta
     4441 acggttactg tggctaaatt ggcttcagct agacataaca tgcttgaaca gtataaaatt
     4501 aatagcatta taatttgcat tgtcgccatt gtactttgct cagtattaag tccgctgtta
     4561 atcagaacgg gattacgaga gatcaaaaag ttgagtggtg taacggaagc gctgaattat
     4621 aacgatagcc gagagcctgt tgaggttagc gcattaccga gagaactaaa acctcttggg
     4681 caggcgttga ataaaatgca tcaagcctta gtcacagatt ttgaacgcct aagtcaattt
     4741 gctgatgatc tcgctcatga acttagaacg cccattaatg cattactggg tcagaatcag
     4801 gttacgctca gtcaaaccag aagtatcgct gaatatcaaa aaacaattgc cggtaacatt
     4861 gaagagttgg aaaatatttc gcggttaaca gagaacatac tgtttcttgc ccgggcagat
     4921 aaaaacaatg ttttggtgaa actggactcg ctttctctca ataaggaagt cgaaaatttg
     4981 ttggattatc ttgaatatct ttcagacgag aaagagattt gctttaaggt cgagtgcaat
     5041 cagcaaatct ttgcggataa aattttactg caacgaatgt tatcgaatct tattgttaat
     5101 gccattagat attcaccaga aaaatcgcgt attcatataa ccagttttct tgataccaac
     5161 ggctatctta atattgatat cgccagtcct ggaacgaaaa tacatgagcc tgaaaaactc
     5221 ttccgtagat tttggcgggg agataattcg cgtcattccg taggtcaggg acttggtctt
     5281 tctttagtca aagcgattgc cgaattgcat gggggaagtg cttcatatca ctatatcaat
     5341 aagcataatg tgttccgaat tacgttaccg caaagaaatt aacatagggc ttcagtacgc
     5401 cataaattaa ccgtgaaaaa acgcagccac gtcgtgagta ctaacgcggc tgcgattaat
     5461 tatgcgctta cattcaaacg taacagggat taacccgcgt aagctgccag catttcctgc
     5521 gccgccagtt tacccaacgc attcgctgcg aaaggactgt cgccggtgag aagtttacgg
     5581 tccttatgta ctcgcccggt gatgtcgtca ttaatgatgt tcatgcccat tttcttcagc
     5641 tcttcgccga agtaccaggt gagatgacct ggcatatagc caatatctgg cgtttgtttg
     5701 tctgcggcgt ctgggaatgc gcaaatggaa taaccattca ctgggttatc gccgtggcga
     5761 agcgccagaa aagccgccgg gccgtggcaa agggagataa caaaacggtc atttttgatt
     5821 gcccactgta aagcggcagc tacgtcctgg ctttcaggta aaccaataag tgcgccatga
     5881 ccaccaggaa caaagattgc tgcatattcg ctatcagcgt tgaggctggc gacaacatcc
     5941 gcgagtttct tcggattgcg gaacaacgat ttatgctgct caaagaatgg cattactttt
     6001 tcatctttgt gcggcatagc ccagtattca aacttggtca tcagaccgga aatggtcgcc
     6061 acttcgaatt cgaaacctgc agcatggaga tgatacaacg gcagcaacgt ttcaatcgga
     6121 tggttaccgg tcgagaacag ttttccgtta tcggttggca aataacgttc gtccgcggcg
     6181 atcaccagaa ttttatgctt accgcgatac ggttttggat agtc
//
LOCUS       DAJTMK010000208         6187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTMK010000208 DAJTMK010000000
VERSION     DAJTMK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.212"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            144..347
                     /locus_tag="OO135_003692"
     CDS             144..347
                     /locus_tag="OO135_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5399369.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            355..1287
                     /gene="aaeA"
                     /locus_tag="OO135_003693"
                     /pseudo
     CDS             355..1287
                     /gene="aaeA"
                     /locus_tag="OO135_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            1293..3260
                     /gene="aaeB"
                     /locus_tag="OO135_003694"
     CDS             1293..3260
                     /gene="aaeB"
                     /locus_tag="OO135_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5399370.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTRRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3352..3624
                     /locus_tag="OO135_003695"
     CDS             3352..3624
                     /locus_tag="OO135_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5399371.1"
                     /translation="MNIYTFDFDEIDSQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3680..3943)
                     /gene="yhcN"
                     /locus_tag="OO135_003696"
     CDS             complement(3680..3943)
                     /gene="yhcN"
                     /locus_tag="OO135_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5399372.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4308..4778)
                     /gene="argR"
                     /locus_tag="OO135_003697"
     CDS             complement(4308..4778)
                     /gene="argR"
                     /locus_tag="OO135_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5399373.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5213..6151
                     /gene="mdh"
                     /locus_tag="OO135_003698"
     CDS             5213..6151
                     /gene="mdh"
                     /locus_tag="OO135_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5399374.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         1512 a         1442 c         1597 g         1636 t
ORIGIN      
        1 tagataacag cataacagtt acgctaatat attaaatatc aatctacagc aatgttgctc
       61 tcgcccggct ttccatgccc ttctctgcgg cgacagatgc tgaaaataat aacgcctgct
      121 ctctctatac aaccaaggtc aacatgagtc tgtttcccgt tatcgtggtg tttgggctgt
      181 ccttcccacc gatatttttt gaattgcttt tatcactggc gattttctgg ctggtgcgcc
      241 gggtacttgt gccaacaggt atctacgact ttgtctggca tccggcgttg ttcaacaccg
      301 cgctctattg ctgcttgttt tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa
      361 acactaataa gaaaattctc ccgtacggcc atcacggtcg tattagtcat tctggccttc
      421 atcgcaattt ttaatgccta ggtctattac accgaatccc cctggacgcg tgacgcgcgc
      481 tttagcgctg acgtcattgc gatcgcgccg gacgtttctg gactcattac ctaggtgaat
      541 gttcatgata accagctggt gaaaaaagga cagatactgt tcaccatcga ccagccgcgc
      601 tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt attatcaggt actggcacag
      661 gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa
      721 gagatcgacc aggctaacaa cgtactacaa acggttctgc atcagttagc gaaagcgcag
      781 gcgacccgcg atctggcaaa actggatctt gaacgcacgg tgatccgcgc gccagcagat
      841 ggctgcgtga ccaacctcaa cgtctatacc ggtgagttta ttactcgagg atcaacggcg
      901 gttgcgctgg tgaaacagaa ctccttctat gtactggcct atatggaaga aactaagctg
      961 gaaggggtgc gtccggggta tcgtgcagag atcacgccgc ttggcagtaa caaagtgctg
     1021 aaagggactg ttgatagtgt tgccgcaggg gtcaccaacg ccagcagcac gcgtgacgac
     1081 aaagggatgg cgactataga ctctaacctt gaatgggtgc gtcttgcgca acgtgttccg
     1141 gttcgtattc gtctcgacaa ccagcaagag aacatctggc ctgcgggcac cactgctaca
     1201 gtggtggtca ctggcaaaca agatcgcgac gaaagccagg attcgttctt ccgtaaaatg
     1261 gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat tttctccatt gctaaccaac
     1321 atattcgctt tgcggtaaaa ctggcgaccg ccattgtact ggcgctgttt gttggctttc
     1381 acttccagct ggaaacgcca cgctgggcgg tactgacagc ggcgattgtt gccgccggtc
     1441 cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat tcgttatcgt ggctttttgc
     1501 gcatcatcgg cacatttatt ggctgtattg ccggactggt gatcatcatt gcgatgatcc
     1561 gcgcaccatt attgatgatt ctggtgtgct gtatctgggc cggtttttgt acctggatat
     1621 cctcgctggt acgaatagaa aactcgtacg cgtgggggct ggccggttat accgcgctga
     1681 tcattgtgat caccattcag ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt
     1741 gtagcgagat cgttatcggt attgtgtgtg cgattatggc ggatttgctc ttttctccgc
     1801 gatcgatcaa acaagaagtg gatcgagagc tggaaagttt gctggtcgcg caatatcaat
     1861 taatgcaact ctgtatcaag catggcgatg gtgaagttgt cgataaagcc tggggcgacc
     1921 tagtgcgacg caccacggcg ctacaaggca tgcgcagcaa cctgaatatg gaatcttccc
     1981 gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac gctatcgctg acgctgatta
     2041 cccaatcctg cgaaacttat cttattcaga atacgcgccc ggaattgatc actgatactt
     2101 tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac
     2161 gcctgcggag agttatcgcc tggaccgggg aacgggaaac gcctgtcacc atttatagct
     2221 gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa
     2281 tcaacgccac cgaagaagag atcctgcaag gcgaaccgga agtcaaagta gagtcagccg
     2341 aacgtcatca tgcgatggtt aacttctggc gaaccacact ttcctgcatt ctgggcacgc
     2401 ttttctggct gtggacgggc tggacttccg gcagtggtgc aatggtgatg attgcggtag
     2461 tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt ggcgatcgac tttatctacg
     2521 ggacgctggc cgcgctgccg ttagggctgc tctacttttt ggtgattatc cctaataccc
     2581 aacagagcat gttgctgctg tgtattagcc tggcagtgct gggatttttc ctcggtatag
     2641 aagtacagca acggcgactg ggctcgatgg gggcgctggc cagcaccata aatattatcg
     2701 tgctggataa cccgatgact ttccatttca gtcagtttct cgacagcgca ttagggcaaa
     2761 tcgtcggctg tgtgctcgcg ttcaccgtta ttttgcttgt gcgggataaa tcgcgcgaca
     2821 ggaccagacg tgtactgctt aatcagtttg tttctgccgc tgtttccgcg atgactacca
     2881 atgtggcacg tcgtaaagag aaccacctcc cggcacttta tcagcagctg tttttgctga
     2941 tgaataagtt cccaggggat ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc
     3001 accagcgcct gcgtgatgcg ccgatcccgg ttaacgagga tttatcggcg tttcaccgac
     3061 aaatgcgccg cacagcagac catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt
     3121 actttggcca gttgctggaa gaacttgaaa tctaccagga aaagctacgc atctggcaag
     3181 cgccaccgca ggtgacggaa ccggttcatc ggctggcggg tatgctccat aagtatcaac
     3241 atgcgttgac cgatagttag gtcaaaaccg acgccaaaag tgtcggtttt ttcatggcta
     3301 tacttagcga tgcacggcag aactcgccgc gaaacgtgac ggtggcaata gatgaatatt
     3361 tatacctttg attttgatga gattgatagt caggaggatt tttatcgtga ctttagccaa
     3421 acctttggtc tggcgaaaga taaggtacgc gatctcgact cactatggga tgtgttaatg
     3481 aacgatgtcc tgccgctacc acttgagatt gaatttgttc atctgggaga gaaaacgcgt
     3541 cgccgttttg gcgcgttaat attgctgttt gatgaggcag aggaagagct ggaagggcat
     3601 ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc cgaaccgggg gcaatatcgt
     3661 cggacaagac gatgagggtt tatttgtaca gttcagccgt agcgtgccag gtgtcaccgc
     3721 tacgagcttc agtaatctgg taggccgttg cgcctttctc ttccgctttt ttgttcagca
     3781 tttcacgcat atccattggc gaagacgcca caccacttac ggatacggtc ccgattgctt
     3841 cacgattttg tgcttgtgca gcatcaatgg agtcggcagc gaatgcaccg aaagagagaa
     3901 cagaaagtac gcttaatgca gctacagtgg ttttgatttt catgattttt acctcgacat
     3961 aatcttttag ctgggccttt gtttcgtgac ccatatcaca aaatcaagca tacactaatt
     4021 attgaaccaa ttaatatcac gctaactgtt tcagttgata ctaagtgtta aaaatgtgaa
     4081 ttttaataac aaaaaagaat taaaaacacg gtcatttatt tttaattcta gtattttcaa
     4141 ttagataatg aattttgcat aatgtgcttt ttttaccgta tgtattcatt atgtgaatga
     4201 catgtcgcag taaaacgcac tattcgttaa attaatcgca ggggaaagca gggggttgag
     4261 agggataagc aacattttcc ccgccgccag aagcgacggg gcagagatta aagctcctgg
     4321 tcgaacagct ctaaaatcgc ttcgtacagg tctttgacgg tgaaaccgtt agcaggggtg
     4381 gtaaagatgg tgtcatcgcc agcgatggtg cccagaatac cttctgcttt gcccagtgag
     4441 tccagcaggc gagcaattaa ctgcgccgcg ccagggctgg tatgaatcac gacaactgca
     4501 tcgttgtagt cgatatccag caccagattc ttcaatggac tggaggtggt tggtacaccc
     4561 agttcagctg gcaggcagta aaccatttcc attttggcat tgcgtgtacg tacagcacca
     4621 aacttggtca acatccgcga gactttagac tgattaatat tgtcaaagcc ttgctcctgc
     4681 aacgcggcga cgatttcgcc ctgggagcta aatttctctt ctttaagtaa tgctttaaat
     4741 gctttaacta gttcttcttg cttagccgag cttcgcataa gtcacccgat atggtggttg
     4801 atacaacatt attgtgcata cagatgaatt tttatgcaaa cagtcagccc tgaagaaggc
     4861 tgaaataatg ttatgaaaga gcgggatttt atcaaatttc gttattgaga aacatgcctg
     4921 cgtcacggca tgcaaattct gcttaaaagt aaattaattg ttatcaaatt gatgttgttt
     4981 tggctgaacg gtagggtata ttgtcaccac ctgttggaat gttgcgctaa tgcataagcg
     5041 actgttaatt acgtaagtta ggttcctgat tacggcaatt aaatgcataa acgctaaact
     5101 tgcgtgacta cacattcttg atatgtggtc attgtaaacg gcaattttgt ggattaaggt
     5161 cgcggcagcg gagcaacata tcttagttta tcaatataat aaggagttta ggatgaaagt
     5221 cgcagtcctc ggcgctgctg gcggtattgg ccaggcgctt gcactactgt taaaaaccca
     5281 actgccttca ggttcagaac tctctctgta tgatatcgct ccagtgactc ccggtgtggc
     5341 tgtcgatctg agccatatcc ctactgctgt gaaaatcaaa ggtttttctg gtgaagatgc
     5401 gactccggcg ctggaaggcg cagatgtcgt tcttatctct gcaggtgtag cgcgtaaacc
     5461 gggtatggat cgttccgacc tgtttaacgt taacgccggc atcgtgaaaa acctggtaca
     5521 gcaagttgcg aaaacctgcc cgaaagcgtg cattggtatt atcactaacc cggttaacac
     5581 cacagttgca attgctgctg aagtgctgaa aaaagccggt gtttatgaca aaaacaaact
     5641 gttcggcgtt accacgctgg atatcattcg ttccaacacc tttgttgcgg aactgaaagg
     5701 caaacagcca ggcgaagttg aagtgccggt tattggcggt cactctggtg ttaccattct
     5761 gccgctgctg tcacaggttc ctggcgttag ttttaccgag caggaagtgg ctgatctgac
     5821 caaacgtatc cagaacgcgg gtactgaggt ggttgaagcg aaagccggtg gcgggtctgc
     5881 aaccctgtct atgggccagg cagctgcacg ttttggtctg tctctggttc gtgcactgca
     5941 gggcgaacaa ggcgttgtcg aatgtgccta cgttgaaggc gacggtcagt acgcccgttt
     6001 cttctctcaa ccgctgctgc tgggtaaaaa cggcgtggaa gagcgtaaat ctatcggtac
     6061 cctgagcgca tttgaacaga acgcgctgga aggtatgctg gatacgctca agaaagatat
     6121 cgccctgggc gaagagttcg ttaataagta attgattagc gaataataaa aaaccggagc
     6181 acagact
//
LOCUS       DAJTMK010000209         6041 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTMK010000209 DAJTMK010000000
VERSION     DAJTMK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.213"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(87..1073)
                     /gene="araH"
                     /locus_tag="OO135_003699"
     CDS             complement(87..1073)
                     /gene="araH"
                     /locus_tag="OO135_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5399375.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVICGLILLAAVIFDRYKQKAKRTV"
     gene            complement(1088..2603)
                     /gene="araG"
                     /locus_tag="OO135_003700"
                     /pseudo
     CDS             complement(1088..2603)
                     /gene="araG"
                     /locus_tag="OO135_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
     gene            complement(2673..3662)
                     /gene="araF"
                     /locus_tag="OO135_003701"
     CDS             complement(2673..3662)
                     /gene="araF"
                     /locus_tag="OO135_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR5399376.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MVITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKVQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            4376..4879
                     /locus_tag="OO135_003702"
     CDS             4376..4879
                     /locus_tag="OO135_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5399377.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4958..5209)
                     /locus_tag="OO135_003703"
     CDS             complement(4958..5209)
                     /locus_tag="OO135_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5399378.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(5324..5410)
                     /gene="azuC"
                     /locus_tag="OO135_003704"
     CDS             complement(5324..5410)
                     /gene="azuC"
                     /locus_tag="OO135_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5399379.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5673..5996
                     /locus_tag="OO135_003705"
     CDS             5673..5996
                     /locus_tag="OO135_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5399380.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         1582 a         1547 c         1367 g         1545 t
ORIGIN      
        1 ggtcgccatt tgcaacgaag aacagccaac cgttatggcg gttggctgtg gtgggaaaaa
       61 acgctaaatt gttgcagaaa aaagcatcag acagtgcgtt tcgctttttg cttgtaacgg
      121 tcgaagatca ccgctgccag caggattaag ccgcaaatca cgtactgcgc gaaaggagaa
      181 atattaagca ggttcatggc gttttccacg gtgcctaaaa ttaagatacc cgccaccaca
      241 tatgagattt ttccgatgcc tcctttcaga gaaacgccac ctaaaacgca ggcggagata
      301 acaatcagct cgtaaccaat cgacgtcatt ggctgcccac tggtcatacg tgaagccaga
      361 ataattccgg ctatcgcaga taccaggcca gagagaacaa agataataat tttggtgcga
      421 acaaccggta cacccgccag acgcgcagcc tcttcgttcc cgccaatcgc cagggtgtta
      481 cgaccaaagg tggttttatt cagcaacaaa ccaaagataa tcagacacgc gacggtgagc
      541 cagattggcg caggcagacc gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct
      601 tcgataccga ccgctttacc gtctgaaatg atgtatgcca gacctcgaac aatctgcatc
      661 gttgccagtg ttgtgatcag agcatttatt ttcagtttgg cgataacaaa gccattgacc
      721 aggccacaga gaatgccaag cagcaacccc gctgccacgc caatccacag gctttcagtc
      781 aggttgataa ccaccgccgt ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg
      841 tcaaagtcac cggaagcaag gcagaacaac atgccacacg ccaccatccc cgacatggaa
      901 attgccaggc ccaacccttt catattaatg aaggtggcaa aatttgggac aaaaatggca
      961 caggcgataa agagcaccgc aaacaccacc agcatgccgt actgatccca gatacgcccg
     1021 aagctgaatg acgacttagg tgcgccagaa cccgatgtag aaacagaaga catcatactc
     1081 tccttactca ggcaactgcc tggctgactt taggcatcgc aaggctcagt gcctgacgct
     1141 catctgcctg ctcgtgtaac aattcaccgg cgatttcacc ttcccgcatc accacaatcc
     1201 ggtcggcaac gccgaggact tcaggtaagt cgctggaggc aaacagcacc gccacaccct
     1261 gcgccgccag cgcataaatt acgttatata tttcgtgctt agcaccaaca tcaatgccgc
     1321 gcgtaggttc atccagcaaa atgaccttca tctcttccga taaccagcgg cccagaatgg
     1381 ctttttgctg atttccgcct gagagattca tgatcagttg ctcagcgccc ggcgttttga
     1441 tgttgagcga acgaatgtgc tgatcggcat tgttttcttc ccaaccgttg ttgattacac
     1501 aaccgccgag cacatgtttg cgtctggcac tgatgttgat attgtcgcga acggagtgca
     1561 cgggaataat gccttccgct ttgcgatctt ccgggcagag catcatgcct gcggcaatgg
     1621 cgtggctcgg tttacgaata tcgatcggct gttggtcgat ataaacctga ccggcggtga
     1681 tttgcgtccc gccaaacaag cctttcatta attcgctacg ccccgcccct accagaccaa
     1741 acagcccaac aatttcacca ctgcgaaccg ccagacttat tggcgtacgc acgcctggtg
     1801 ctttcacagc atcaagacgc aggcgctcct cgccataact acgaggttgc cagccgtaga
     1861 tatcgccaat gtcgcgcccg accatcgcct gcaccagcgc gtcgtggtca acctgctgca
     1921 tatcggtaaa ggttttgaca taacgtccat ctttaaagac agtgatggca tcgctgaggg
     1981 caaatatttc ttccatacgg tgagaaacgt ataagatcac ccgcccctct tttcgcagtt
     2041 cacgaataac gcgggaaaga ttgtcgattt cgcgggcaga aagggagctg gttggctcat
     2101 caaaggcgat aattttagcg ttacgcgcca gcgccttggc gatttcaacc atctgccact
     2161 gaccaatgga gagatatttc agcggcgtgt ccgggtcaat atccatacca agatgtttaa
     2221 gttgtaaacc cgcctcataa ttcagcaatg agcgattcac aatgccgcct ttatgcggca
     2281 gctggccgag atagatgttt tccgcgacgg tcatttccgg cacgagatgc agttcctggt
     2341 aaataatcgc cacacccgcg ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc
     2401 cattaatcac tacagaaccc gtggttggcg catagttgcc gctgaggatt ttttaagaga
     2461 gttgattttc ctgcgccatt ttcacccatc aacgcatgaa cctgaccggc atagcagtca
     2521 aaactaatat ccgtcagcgc cttaacgccg ggaaacgttt taccgatgcc gcgaaatgag
     2581 agatacgggg tagactgttg cataacgtct ccgtgaatca ctggtcgttc acaccccctc
     2641 tgcatcatgc agaggggaat ttttccggca aattacttac cgcctaaacc ttttttctcc
     2701 agttcttctt taaagttgtc acgcgtgatc agcaccacgt cggtaacttc ggtaaatttt
     2761 ggcggttcaa cgtcttttgc tacccagttg taaagcattt cgctggattt atagccatgt
     2821 acgtccgggc ttggcagcag ggaaccgtag aagccggttg cctgtacttt agacagttcg
     2881 ctcaccgcat ccacaccgtt aatgccaatg ccgataatat cggcagcttt aaagccctga
     2941 ccttccgtcg cgcgtacgcc gcccagcacg gtgctgtcgt tcataccgac gatcagccaa
     3001 tgtttaactt caggatgttg aaccagcatt gagttggcag cgtcaaatgc ccccgggatg
     3061 tcgttagatt tggtaggtac ctgataaatt tgtttttccg ggaatccggc cgctttcagc
     3121 gcatccatag atcccgtagt acgccggcgg gcggtatcca gttcgttggc ggtaatcacc
     3181 atcaccgcgc tttctttgac atcccagcca cgtttctgca tctctttata cagttcctga
     3241 ccctgacgtt cgccaatttt agtcgccgcc atcatcacca gcggaacggt atccattggc
     3301 ttacctttgg cgttaacaaa ctgatcatcc acggcaatga ctttcatatc gtagccacgc
     3361 gctttcgcga cgatggcaga gccgagtttg gggtccggag tacaaataac gaaacctttt
     3421 gcgccactgg cagccaggct gtcgatcgcg ttcaatgttt tttcgccatc cggcacggca
     3481 atcttaataa cctcaaaccc taaatccttc ccggctttat cggcaaactt ccattcggtc
     3541 tggaaccacg gctcttccgg ttgcttcacc agaaaaccga gcttcaggtt ctccgccata
     3601 gcggattgtg acataacggc tgccagacca atggctgcca gggctttagt aaatttgtgc
     3661 atggttctct ccagctttag tgtcgttttg tgtagggcaa aatcgaatga cattcgttaa
     3721 attaatcgga aaacaaagca ttacctttta actaaaagat aagtgaccgt gttgacatag
     3781 ttttagcgag aaattaattc tccataggag agcaatatca catcgcagaa ttacagtgag
     3841 aacgtgcata aatttagcgg gaaaagacat aagggaaagc caatttgtca gacaaattgt
     3901 cgaatgcaca gcagattaat ccataagatt agcttggaaa tccttgttgt ctttggtacc
     3961 catgcgggat gtcttctttt taaccagtca ataggccgca ttacctggcg ttgagttttt
     4021 gaaatggtgt aataaccgca actcaaagat gtggaaaatg cacgtcattc atttcgccat
     4081 taattatcac tgtgctcatt aattaacaga acacgtataa tgagagccat ctcgcaaaaa
     4141 tgaaaaaacg ttttataaaa tcatcatttc atcaatgaaa tgtaaaaata tataaacttg
     4201 atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga tcatcgtcag
     4261 aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca aacagtaatt
     4321 atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat taggtatggc
     4381 aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt acgcatccaa
     4441 tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca ccgccacttt
     4501 ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta actttatgaa
     4561 gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa aactgaactc
     4621 tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta gtacgttggc
     4681 acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt tcctgcgcga
     4741 tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg atgaagtgcg
     4801 cagtgcgaaa cttgctggta tgtgcccagt gcagaccgac caacatgttc tgaatgtcgt
     4861 gtcgcatcag ctgcattgat catacatcgg cgctaatgca ttgcgccgat gaagtttttg
     4921 agaaaccgct gcctcatctt tttgaagcag cggtttttta atgggattcg ccttgtgggg
     4981 taaacttgag ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg
     5041 cggcggcggg atggttacta aagtcgatat tcttcagctg actggacatt ttttcacgaa
     5101 cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag
     5161 caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct
     5221 gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca
     5281 ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc
     5341 ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc aggatcttgc
     5401 gcagtttcat cgcggcactc cgaccatttg ttatacatgt gttattttct ttgcgcttat
     5461 aatatgacaa ccatcacaaa aatcaatctt tatgtgatga aaatcacaca aataccccct
     5521 taatgttata aaaatgataa tcaaaaaaca gcccccccat ttctgacacc tacagatggc
     5581 aagaaatagc gcctgccagg cgtcttttcc ggtcattgtc gcagcgctgt aacgcgtaaa
     5641 atagtgcttt cttttactct tctggctgga ccatgagact tctgattctg actctttcat
     5701 taataacgct ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg
     5761 caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc
     5821 gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga
     5881 tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata
     5941 cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat
     6001 tttaatcagc gaggggatct tcgctgatta aagaaatagc g
//
LOCUS       DAJTMK010000210         5998 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTMK010000210 DAJTMK010000000
VERSION     DAJTMK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.214"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            191..865
                     /locus_tag="OO135_003706"
     CDS             191..865
                     /locus_tag="OO135_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5399381.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSQGRLINFPEQPEFSEEERKRYNGMAMDYVLVSKKNNKIVCVI
                     ELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPLVAAT
                     KSESVH"
     gene            complement(956..1092)
                     /locus_tag="OO135_003707"
     ncRNA           complement(956..1092)
                     /ncRNA_class="other"
                     /locus_tag="OO135_003707"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1137..1224)
                     /locus_tag="OO135_003708"
     tRNA            complement(1137..1224)
                     /locus_tag="OO135_003708"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1188..1190),aa:Ser,seq:gga)
     gene            1458..2396
                     /gene="ghrA"
                     /locus_tag="OO135_003709"
     CDS             1458..2396
                     /gene="ghrA"
                     /locus_tag="OO135_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR5399382.1"
                     /translation="MDIIFYHSTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPHVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2451..3188
                     /gene="ycdX"
                     /locus_tag="OO135_003710"
     CDS             2451..3188
                     /gene="ycdX"
                     /locus_tag="OO135_003710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5399383.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3212..3766
                     /gene="ycdY"
                     /locus_tag="OO135_003711"
     CDS             3212..3766
                     /gene="ycdY"
                     /locus_tag="OO135_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5399384.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3868..4359
                     /locus_tag="OO135_003712"
     CDS             3868..4359
                     /locus_tag="OO135_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5399385.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4423..5256)
                     /gene="csgG"
                     /locus_tag="OO135_003713"
                     /pseudo
     CDS             complement(4423..5256)
                     /gene="csgG"
                     /locus_tag="OO135_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(5283..5699)
                     /gene="csgF"
                     /locus_tag="OO135_003714"
     CDS             complement(5283..5699)
                     /gene="csgF"
                     /locus_tag="OO135_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5399386.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNNYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5724..5998)
                     /gene="csgE"
                     /locus_tag="OO135_003715"
                     /pseudo
     CDS             complement(5724..5998)
                     /gene="csgE"
                     /locus_tag="OO135_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
BASE COUNT         1495 a         1369 c         1506 g         1628 t
ORIGIN      
        1 attctttgct aatattgtgt gttgttattt ttttgtggaa ggcaagccgc aatggctaaa
       61 tagggatggt tgtaataatc aaaacatgat actaactata tattgatcgt tgcattgttg
      121 gcatgtaaca cgctgcagcg agtaacattg gtaatgaaat ataatcaaca tggataggtg
      181 aaaattaatt atgagcttta tggcttttgt aacttgtttg acaattgcat ttatcgccgg
      241 ttttgctgtt gccagaaaat tgacactccg aaaagttgcc tcatcggtaa tcatcgctaa
      301 atcgcaaccc gtcccttcat ctgcaacttc ccctgccgct aaggcgtcga caaggagcta
      361 tataccgcag aaaacctatc gtgactctcg ttggcaagag cgagatgagt tatttcaggg
      421 aatggttgcc gaaggtattt tattggaaag aaaagagtgg ttaatcactc gttgtgaaaa
      481 aatgtatttc gataatctgg aaaaatggtt tggacattat tgtcatatcc attgccaggt
      541 ctctcagggg cgcttgatca actttccaga acaacctgag tttagcgaag aagaaagaaa
      601 gcgttataat ggcatggcaa tggattatgt tctggtttca aagaagaaca ataaaatagt
      661 ctgtgttatt gagctggatg acccgagtca tgaaagggcg gatcgcatta ttcgtgaccg
      721 ttggttggat acaattatga ctcttgcacg ggtgcctttt gtacgagctt ctgtcactaa
      781 attggaggaa attcctccgg tctgggagac tcgtgacaaa attcgcccat tagtcgcggc
      841 gacaaagagt gaatcggtgc attaactaag tatcccggtc ggggcttctc atccccccgg
      901 tgtgtgcaat atacgaaaaa aagcccgtac cttcgtacga gctcttcttt aaagatggcg
      961 gtgagggggg gattgactcg cttcgctcgc cctgcgggca gcccgctcac tgcgttcacg
     1021 gtctgtccaa ctggctgtcg ccagttgtcg aaccccggtc ggggcttctc atccccccgg
     1081 tgtgtgcaat atacgaaaaa aaagcccgta ctttcgtacg agctcttctt taaatatggc
     1141 ggtgaggggg ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc
     1201 cttcaaccgc tcggacacct caccaaattg ttgttcctgt cttgctggaa cgggcgctaa
     1261 tttagggaaa tcatgacctg aggtcaacaa actttttgaa aaaatcgcgc gtttattcaa
     1321 acttcaatca atgtgtggtt ttaataagcg aaatctgctt tttttgccac cgaccacgga
     1381 tttgttatgc tggtggcctt tgtagatcat aacgataagt gcgaataaat ttcgcacaac
     1441 gcttttcggg agtcagtatg gatatcatct tttatcactc aacgttcgat acccaatggt
     1501 ggattgaggc actgcgcaaa gctattcctc aggcaagagt cagagcatgg aaaagcggag
     1561 ataatgactc tgctgattat gctttagtct ggcatcctcc tgttgaaatg ctggcagggc
     1621 gcgatcttaa agcggtgttc gcactcgggg ccggtgttga ttctattttg agcaagctac
     1681 aggcacaccc tgaaatgctg aacccttctg ttccactttt tcgcctggaa gatactggta
     1741 tgggcgagca aatgcaggaa tatgctgtca gtcaggtgct gcattggttt cgacgttttg
     1801 acgattatcg catccagcaa aatagttcgc attggcaacc gctgcctgaa tatcattggg
     1861 aagattttac catcggcatt ttgggcgcag gcgtactggg cagtaaagtt gctcagagtc
     1921 tgcaaacctg gcgctttccg ctgcgttgct ggagtcgaac ccgtaaatcg tggcctggcg
     1981 tgcaaagctt tgccggacgg gaagaactgt ctgcatttct gagccaatgt cgggtattga
     2041 ttaatttgtt accgaatacc cctgaaaccg tcggcattat taatcaacaa ttactcgaaa
     2101 aattaccgga tggcgcgtat ctcctcaacc tggcgcgtgg tgttcatgtt gtggaagatg
     2161 acctgctcgc ggcgctggat agcggcaaag ttaaaggcgc aatgttggat gtttttaatc
     2221 gtgaaccctt accgcctgaa agtccgctct ggcaacatcc acacgtgacg ataacaccac
     2281 atgtcgccgc gattacccgt cccgctgaag ctgtggagta catttctcgc accattgccc
     2341 agctcgaaaa aggggagagg gtctgcgggc aagtcgaccg cgcacgcggc tactaataaa
     2401 gcatcaggat tcctgctatc cttggcggga attgaataca ggagagagtt atgtatcccg
     2461 tcgaccttca tatgcatacc gttgccagca cacatgcata tagcacattg agtgattaca
     2521 ttgcccaggc caaacaaaag ggcattaaac tttttgcgat caccgatcat ggcccggata
     2581 tggaagatgc tccgcatcac tggcacttca ttaacatgcg tatctggccg cgagtggttg
     2641 atggggtagg gatcctgcgc ggcatcgaag ctaacattaa aaatgttgat ggtgaaattg
     2701 attgcagcgg taaaatgttt gactcgctgg atctgattat tgccggtttt catgagccgg
     2761 tttttgcacc acatgacaaa gcgaccaata cacaagcgat gatcgccact atcgccagcg
     2821 gcaatgtgca tataataagc catcccggaa atcccaaata tgaaatagat gtgaaagccg
     2881 ttgctgaagc agccgcgaaa catcaggtgg cgctggaaat caataattct tcatttttac
     2941 actcacgtaa gggcagtgaa gacaactgtc gtgcggtagc cgcagcggta cgtgatgctg
     3001 gaggttgggt ggcattaggc tcggattctc acaccgcgtt taccatgggg gaatttgaag
     3061 agtgtcttaa aatcctcgac gcggtagatt ttccgccaga gcgcattttg aatgtttctc
     3121 cgcgccgctt actgaacttc cttgaatctc gcggtatggc accgattgcg gaatttgcag
     3181 acctttaatt actagtaatg gaaatatatt aatgaacgag ttttctatcc tctgtcgtgt
     3241 gctgggttcg ctctattacc gccaaccgca agatccttta ctggtgccgc tgtttaccct
     3301 gattcgtgag gggaaactgg ctgcgaactg gccactggag caggatgagt tactgacgcg
     3361 tttacagaaa agctgtgata tggcgcaagt ctctgccgat tacaatgcgt tgtttatcgg
     3421 cgatgaatgt gccgtgccgc catatcgtag cgcatgggtt gaggacgcaa cggaagcaga
     3481 agtgcgcgct tttctttccg aacgagggat gccactggcg gatacgccag ccgatcacat
     3541 cggcacattg ctgcttgcgg cttcctggct ggaagatcag tcaacggaag atgagagcga
     3601 agcactggaa acactgttca gtgagtatct gttaccctgg tgtggtgcgt tcctcggcaa
     3661 agtggaggcc catgcaacca cgcctttctg gcgcaccatg gcaccgctaa cccgtgatgc
     3721 cattagtgca atgtgggacg agctggaaga agattctgaa gagtaaatgt gatcatcgtc
     3781 actcataact gcaacagata atttgttgtt gcataaaatg tgtgctcgat ctcattcatg
     3841 gccgcgtttt ctgctatcat gcgcggcatg aacatacttc tctctattgc aatcacaaca
     3901 ggtattctct ccggtatctg gggatgggtg gctgtttccc tgggcttact aagctgggcg
     3961 ggcttcctgg gctgtacggc ctactttgcc tgcccgcaag gtgggctgaa agggctggcg
     4021 atctccgctg caaccctgct tagtggcgtg gtgtgggcga tggtcattat ttacggtagt
     4081 gcactggcac cacatctgga aattctcggt tatgtcataa ccggtattgt ggcgtttctg
     4141 atgtgtattc aggccaaaca gctgctgctt tcatttgttc cggggacgtt tataggcgca
     4201 tgcgcgacgt ttgccgggca gggtgactgg aaactggtgt taccttcgct ggcgcttggg
     4261 ctggtatttg gttacgcaat gaaaaacagc ggcctgtggc tggcggcgcg tagtgcaaag
     4321 acggctcacc gtgagcagga aatcaaaaat aaagcgtgag gggcactcac gctttcgctt
     4381 aaacagtaaa atgccggatg ataattccgg cttttttatc tgtcaggatt ccggtggaac
     4441 cgacatatgg cggtatttca ccagaatgtc attctgccgt tctgctttat tttgcaaatc
     4501 ccacagacca cggtcgatac catcattaat caggaaaatg acccctgttt cgatagccga
     4561 catcagacac agcataacgg gttcgtttga ggtgtaaccc acttcccctt ccagcaagcg
     4621 ctggtagtca ataaagcgga aaaccccggc ctgaacttca taggaaagta tcgtcttact
     4681 ggtgttcacc gaggagagga tctcaccggt actcacattg acgacacgca ggttcacggc
     4741 aatctgatcg agctggtatt gcgtgtctgc accgatccca aaatatcttg ccccaacacc
     4801 gcccgattta acgttgcttt cataaccgat aatcgaacct tcaaccataa tatttgccgc
     4861 cgttaaagat tgcagcggga ttcggttatt aatggcaacc gtgccgtttt cttgtgccgc
     4921 acgaataatc ttgcgctcgt taagcaggtt ttgtaagccc tagcgctcca gcggtataaa
     4981 ccagcgagaa tctttcaatg ccgtgaccag cattgccgtg gcgctttgcg gaacagcagt
     5041 ggagaagtta cttgccgggt agggtttaaa ttgcccggtt tcgtcctgaa tgttgtatac
     5101 cgaaacaaag attttacccg tcggcgctgg cagatgggtc aaatctttgt agctctgagc
     5161 acgaggcatt aatgtcggtc tggcggcttc tttaggcggg gcggttaagc atccgctcag
     5221 taacatgacg gcaaccaaaa gaaataagcg ctgcatgatt attttcctta tgaagctggg
     5281 gcttaaaaat cggttgagtt attttgtaaa cccgaaacct ggatggtcga ggtttgtccg
     5341 gttttacgat ctgtcacgtt caactgcaat tgaccatcgc ggttggcaat atcgacaata
     5401 taattgttgg tcaccatgcg gcccggttta ccggtattaa tattcgacag tagcccacct
     5461 aaaatttgtg actggatggc ctgagtaaag ttatctaacg ctgagggtgt ttcaatacca
     5521 aagtcatcgt tatagctcgg atctttataa gagttttggg cctgagcgct atttaataaa
     5581 aaagcgccat tatttgggtt accaccaaag tttggattac ggaactggaa agtcatggtt
     5641 ccagcccaac ttaatggcga aataagcatg agtagaacta ctgcatgttt gacacgcatt
     5701 gcagcctccg aacaattttt tatttagaat tcatcatgcg ccaaatcgcc cgtacttaat
     5761 aacgcctgat ttatctggcg acgatttagt gcttcttcag tttgaatcag tgcaaagacg
     5821 acagttttct cgaagtctct tttcaacgga aataaaaaag tctggaaaat aacgtcctga
     5881 ttgaccgtta tagcgatcca gcttccccat cgtgcactgg gcctttcatt aatcgttaag
     5941 ttacccgtat agtcactttc tcatttatca ctaaaggctc ggtaaaaatc atggccaa
//
LOCUS       DAJTMK010000211         5981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTMK010000211 DAJTMK010000000
VERSION     DAJTMK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.215"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            255..617
                     /gene="queD"
                     /locus_tag="OO135_003716"
     CDS             255..617
                     /gene="queD"
                     /locus_tag="OO135_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5399387.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            695..1963
                     /locus_tag="OO135_003717"
     CDS             695..1963
                     /locus_tag="OO135_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5399388.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSHVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMDMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1954..2214
                     /locus_tag="OO135_003718"
     CDS             1954..2214
                     /locus_tag="OO135_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5399389.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2231..2806
                     /locus_tag="OO135_003719"
     CDS             2231..2806
                     /locus_tag="OO135_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5399390.1"
                     /translation="MPLLHLLRQNPVIAAVKDNANLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLITGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2954..3814)
                     /locus_tag="OO135_003720"
     CDS             complement(2954..3814)
                     /locus_tag="OO135_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5399391.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAKVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3811..4590)
                     /locus_tag="OO135_003721"
     CDS             complement(3811..4590)
                     /locus_tag="OO135_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5399392.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(4568..5977)
                     /locus_tag="OO135_003722"
     CDS             complement(4568..5977)
                     /locus_tag="OO135_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5399393.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
BASE COUNT         1463 a         1622 c         1521 g         1375 t
ORIGIN      
        1 gaagataagg ttaacggggt gaacggtgtg gattaaagac gggatagcga taactaataa
       61 ccaaatcgac taacctgttt tagcaatagt ctttaacaac aaaatagatt aaccaaccca
      121 atgaaaaaca aatgaattta gccaatcatt aagataaatc agcgattttg cgcaacaaag
      181 tcgttttaga taatgcgaaa aaacagcctt tccggtactc tacggcggtt ttatcgtcct
      241 gtagagaaat tatgatgtcc accacgttat ttaaagattt caccttcgaa gccgctcacc
      301 gcttaccaca cgtcccggaa gggcataaat gtggtcgcct gcacgggcat tcctttatgg
      361 tgcgactgga aattaccggg gaagtcgatc cgcatacggg ctggattatc gatttcgctg
      421 aactaaaagc ggcgtttaag ccaacttatg agcgcctcga tcaccattat ctcaatgata
      481 ttccaggtct ggaaaaccca actagcgaag ttttagcaaa atggatttgg gatcaggtta
      541 aacccgttgt gccgctgtta agtgcggtga tggtaaaaga aacctgcacc gctggttgta
      601 tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt tcccttaagt aacgctatgt
      661 tagggtattg tgttcaggat atctggggca tgacatggaa gacgactgcg acattattat
      721 tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc tgcgcgcgag cgggtttatc
      781 cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa aatctttccg gcggacggtt
      841 atatacccat gcactcgcgg aactcctccc tcagtttcat ctgaccgcgc ctcttgaacg
      901 acgcatcact cacgaaagcc tttccctgtt aacgcccgat ggcgtaacga cgttttccag
      961 cttacagccc ggcggtgaat cctggagtgt attacgtgca cggttcgatc cgtggctggt
     1021 tgccgaagcc gaaaaagaag gtgtcgaatg tatccccgga gcgacggtgg atgcactgta
     1081 tgaagaaaac ggcagagtct gtggcgttat ttgtggtgac gatattctcc gcgcccgtta
     1141 tgtggtgctg gcagaaggtg ccaacagcgt cctggctgaa cgtcacgggt tagtgactcg
     1201 tcctgctggc gaagcgatgg cgttggggat caaagaagtg ctgtcgctgg aaacatccgc
     1261 tattgaagaa cgttttcatc tggagaataa cgaaggcgca gcgttgctgt tcagcggcgg
     1321 aatctgtgat gacttacccg gcggcgcatt tctttatact aatcaacaaa cgctctcgtt
     1381 agggattgtt tgcccgctct cttcccttac gcaaagtcat gttccggcaa gcgagctgct
     1441 gactcgcttt aaagcgcatc cggcagtgcg cccgcttatc aaaaacacgg aatcactgga
     1501 gtatggtgcg catctggtgc cagaaggtgg cttgcacagt atgccggtgc aatacgccgg
     1561 taacggctgg cagctggtgg gcgatgcgtt gcgcagttgc gtcaataccg gaatttccgt
     1621 gcgcggcatg gatatggcgc tgactggcgc gcaggcggca caaacgctga taagcgcctg
     1681 ccagcaccgc gagccgcaaa atctgtttgc gctttatcat cacaacgtag agcgcagcct
     1741 gctatgggat gttctacaac gttatcagca tgttccggcg cttttgcaac gccctggctg
     1801 gtatcgggcg tggcctgcgt taatgcagga tatttcccgc gatttatggg atcagggtga
     1861 taaacctgtt ccaccgctgc gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg
     1921 gcatctggcg ggcgatgtta tcaggagtct gcgatgtctg tagcccataa tctctggcgc
     1981 gttgctgatg cgccgcacat tgttccggct gactccgttg agcgtcagat ggtacaatgg
     2041 ttgattaacg cctgtccggc aggtcttttt tcgctcacac cggaaggtga cttacgcgtt
     2101 gattatcacg gctgcctgga gtgtggcacc tgccgtttgc tgtgcgacga atccacacta
     2161 caacagtggc gctatccgcc ttccggattc ggcatcacct accgctttgg ataaataata
     2221 aagataattt atgcccctct tacacctgct ccgccagaat ccggtgattg ctgccgttaa
     2281 agataatgcc aacctgcaac tggcaatcga ctctgaatgc cagtttattt ccgtgttgta
     2341 cggcaatatc tgcaccatca gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc
     2401 ttttattcat gttgatttgc tggaaggcgc gtcaaataaa gaagtcgtga ttcagttttt
     2461 gaaactggtg accgaggcgg acggcattat cagtaccaaa gcctcaatgt tgaaagcggc
     2521 aagagcggaa ggttttttct gtattcatcg cctgtttatt gttgattcaa tttcgtttca
     2581 caacattgat aagcaagttg cgcaatcgaa tccggattgt attgagatcc tgccaggctg
     2641 tatgcccaaa gtgctgggct gggtgacaga gaaaatccgc caaccgctga ttaccggtgg
     2701 gctggtgtgc gatgaagaag atgcgtgtaa tgcgattaac gcgggtgtcg tggcgctttc
     2761 caccacgaat accggggtct ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa
     2821 ccaattgaat ttggtttgat ttgtaggccg cacgccacat ccgacattca gcgcctgatg
     2881 cgacgcttga cgcgtcttat caggcctaca agtccccagg ccagaaccgt aggcaggatc
     2941 atccggcaac acattattgg caatctgcgt gaatgtttgt caccagtgcc tccagcacca
     3001 ctttccagtc atcaaccacg ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat
     3061 gattaatcgc caccacaaac ttgctattgc gtacgcccgc cattaacgct gctgcgccgg
     3121 aagcgccaac aacaatacac acctcaggtg ccagcaaatg tccggaaata ccaatcactt
     3181 tttcagcatc aacgccaccg ttcatcactc gcgcccgact gtagcccact ttggctccca
     3241 gcttttctgc cagcatagcg atttcctggt tatctgcctc tccgccctgc cccaccacca
     3301 gcacccgtct ggcctcagct aatgggtcgc gagtaacatt tttcagatcc tctgtgctaa
     3361 ccagccagtc gggaagcgca cccgggacaa tgttcagttg ttgtattcca gagggcaacg
     3421 tggcgttttt cgctgctccc gcctgacgcg ccagggaaag gcatagcggg cgtttttctg
     3481 tttgcagcgt cgccgttagc gcatttcccc agtgggattt gcgcacgctt acggttggaa
     3541 tatctaacga agtcacctgg cagatgcttg caccatgtaa acgccaggcc agccgggtcg
     3601 acagttcatc gccaaacgtt cccggcggaa agagaacaac gtccgcaggc gttcgctgcc
     3661 actgttccac caacgcatcc agcacctgtt ccgccactac aggctgaggt tcaatctgcc
     3721 agtgcgccag ggtacaacca ctgaaatcct gcgccgccag ccagcttgct attgcggcat
     3781 tctcctgatt tattgtcaca attgcgatat tcatggctgc atcctttggc gtaaatagtc
     3841 ctgccatagc ttttgagctt tttctgctac cgtttgccca tcaatcagcg tcgctccgcg
     3901 tcgttgctct gctcttgcca gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt
     3961 tttacggata atttctgctt tccccgcagc cattcgctgg cgcattcccg gtactggcaa
     4021 ggccacttca ccgcactgac gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg
     4081 caacccatgt tcagtgcgct gttcgagggt gataaacagc gcgtcgagag tgaaacgctc
     4141 cacctgggtg aagcaggtcc agcccagcat ttccgccagc aaaaatggcg tttgcccatt
     4201 ctgcccttcg ctgctttggc agccggtaat tatcagatcc agtggattct gatgctgcca
     4261 ttcggcaata tggcgagcga caaattccgg cgcaaagcgc agatctgccg ccgtctccag
     4321 caatacagct tcctcaaacc caagagccat gagatagcgc agccagtgca atgcccgttc
     4381 atcccccatg ctcaacgcgg ttaaagacat cggcgtaccg ttttttctct gcgccaacag
     4441 cagcgcggca gcggcctgtt catcagcacc gagtaaactt cgcagtagcg aaatatccgg
     4501 tccgctttta ccctgagccg ccgcctgcca ctctttttcc gccagcattc cggcatccgg
     4561 ttcggcttta aacgctaaca gaatgttcat tcgcacctcc tacatttccc gccgccacca
     4621 gcgggagggc tttagtttct ggtgcccata gccaggtcac aacaaaacca accaacaaca
     4681 ctgtcgccag gagcaataag gtgacttgca ttccccactg cgccagcacc cacggcagca
     4741 ggccagtact taccgccgcg ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg
     4801 agcgaatgtc agtaggaaaa ctttccgcag gcaaaatgcc caccagatta ctgactgccg
     4861 aaatggtggt gctgaagaga acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac
     4921 aggccattac taccagcgtt gccgccagca gcaaaaaact tcccagcaaa aatttgcgat
     4981 gtgccagcag gtgcgtcaga actaatccca gcagcgcgcc cacaattaac aacgcattaa
     5041 gcatcaggct ggcagtcagc gcatcttcca gaccaatagt ctgggcgata gttggcagcc
     5101 aggtataaat cacaaaccat gggattacga gacagacaaa gaagacgctg ttaaacgccg
     5161 tgcgccgcca gtaacgcgaa gagaacaagg ttttgatgtg tttatgggtc gccgttacca
     5221 cttcatcgcc cagtaaaaca tggggaccaa aatagcgatg cacgatagcg tgagcttctg
     5281 caaaacgccc ctggcgcagt agccagcgtg gtgattctgg cgttccccag cgtaataacg
     5341 taatcaacaa cgcgggcaga gctgccgatg ccagtagcca gcgccaggcc tccgggtttt
     5401 cggaaataaa gtgatgtccg gcaatacttg ccagcacata gcctacggtc cacaccacgc
     5461 tgaatgcgcc cagcaaaata ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt
     5521 gacctactga ataatcgcct cccagaccaa tgccaatcaa aatgcgcagt ccaataagat
     5581 gctctggcgt ggtggcaaaa aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga
     5641 aggtgaagat tttttgccga ccaatatggt cggagatcca cccaagaacc aggctgccaa
     5701 ggaacaaacc aaggagcgcc gagccgccga tcattcccgc cataaacggc gtcagttgca
     5761 tggcgggcgt aagctgaata atggcgtaac caatgacgcc gagaacataa ccgtcggtca
     5821 ggtgtgcgcc gaaagtgagc gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat
     5881 ccattcgcac cggtgaagtg ttcatcttta ccccttcatt gtccggtaat aagaacggct
     5941 tctcgcctga gaagccgaaa aggcaacgcc ccgtcatcgc g
//
LOCUS       DAJTMK010000212         5978 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTMK010000212 DAJTMK010000000
VERSION     DAJTMK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.216"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO135_003723"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO135_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5399394.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO135_003724"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO135_003724"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5399395.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO135_003725"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO135_003725"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5399396.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO135_003726"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO135_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5399397.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            5760..>5978
                     /locus_tag="OO135_003727"
     rRNA            5760..>5978
                     /locus_tag="OO135_003727"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1510 a         1411 c         1618 g         1439 t
ORIGIN      
        1 ctcaagggct gtcgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcagca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggtgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtagctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag tgtattatac
     5581 acaccccgcg ccgctgagaa aaagcgaagc ggcactgctc tttaacaatt tatcagacaa
     5641 tctgtgtggg cactcgaaga tacggattct taacgtcgca agacgaaaaa tgaatgccaa
     5701 gtctcaagag tgaacacgta attcattacg aagtttaatt ctttgagcat caaactttta
     5761 aattgaagag tttgatcatg gctcagattg aacgctggcg gcaggcctaa cacatgcaag
     5821 tcgaacggta acaggaagca gcttgctgct tcgctgacga gtggcggacg ggtgagtaat
     5881 gtctgggaaa ctgcctgatg gagggggata actactggaa acggtagcta ataccgcata
     5941 atgtcgcaag accaaagagg gggaccttcg ggcctctt
//
LOCUS       DAJTMK010000213         5937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTMK010000213 DAJTMK010000000
VERSION     DAJTMK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5937
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.217"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            142..1242
                     /locus_tag="OO135_003728"
     CDS             142..1242
                     /locus_tag="OO135_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5399398.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTLNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1267..3662
                     /gene="torZ"
                     /locus_tag="OO135_003729"
                     /pseudo
     CDS             1267..3662
                     /gene="torZ"
                     /locus_tag="OO135_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(3860..4831)
                     /gene="cmoB"
                     /locus_tag="OO135_003730"
     CDS             complement(3860..4831)
                     /gene="cmoB"
                     /locus_tag="OO135_003730"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5399399.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLIRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4828..5571)
                     /gene="cmoA"
                     /locus_tag="OO135_003731"
     CDS             complement(4828..5571)
                     /gene="cmoA"
                     /locus_tag="OO135_003731"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5399400.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5612..>5937)
                     /locus_tag="OO135_003732"
     CDS             complement(5612..>5937)
                     /locus_tag="OO135_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5399401.1"
                     /translation="RLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMNG
                     AETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWEL
                     VFSLR"
BASE COUNT         1613 a         1416 c         1559 g         1349 t
ORIGIN      
        1 aattaatgat tacattgtaa taaatcatat tctttatcga ttatttcagg cagtgttgtg
       61 ccgagttatg caagcggtta attcgttgtg tatttaatta tccaatgatt tcggtgttct
      121 ggaatttatt tagaggaatc tatgcgaggg aaaaaacgca ttgggttatt gtttttgctg
      181 atagcggttg tggttggtgg cggcgggtta ttgctggcgc aaaaagcctt acataaaacg
      241 tcggatacag cattttgcct ttcctgccac tcgatgagta aaccttttga ggaatatcag
      301 ggaactgtcc acttttcgaa ccagaaaggg atacgtgcgg aatgtgcaga ttgccatatt
      361 ccaaagtcag ggatggatta tttatttgct aaattaaaag catctaaaga tatttatcat
      421 gaatttgtta gcggcaaaat agacagtgac gataagttcg aaacacatcg ccaggaaatg
      481 gccgaaacag tatggaaaga attaaaagca actgactctg caacgtgccg tagttgccat
      541 tcttttgatg ccatggatat tgcctcgcaa agtgaatctg cgcagaaaat gcataacaaa
      601 gcacaaaagg gcggcgaaac ctgtatcgat tgtcataaag gcattgccca ttttccgcca
      661 gaaataaaaa tggatgacaa cgcggcgcat gagctggaaa gtcaggccgc tacttcagtg
      721 actaatggcg cacatattta tcctttcaaa acttctcgca taggcgagct ggctaccgtg
      781 aatcctggta ccgatctcac cgtcgttgat gccagtggca aacagccgat cgttctgttg
      841 cagggttatc aaatgcaggg tagtgaaaac acgctctacc tggcggcagg tcaacggctg
      901 gcgctagcca cattaagtga agaaggtatc aaggcgctca cgctaaacgg ggaatggcag
      961 gctgacgaat acggcaatca atggcgtcag gcgtctttac agggtgcgct taccgatccc
     1021 ggattagcgg accgtaaacc gctatggcaa tacgctgaaa aacttgacga tacctattgc
     1081 gctggttgtc atgcccctat tgccgccgac cattacaccg tcaatgcgtg gccgtccatt
     1141 gccaaaggaa tgggggcacg aaccagcatg agcgaaaacg aactggacat tttaacgcgg
     1201 tatttccagt acaacgccaa agatattacc gagaaacagt gatcctcgtt gatcagggag
     1261 gaagttatga cattaacaag acgtgaattt attaaacaca gcggtattgc cgctggagcg
     1321 ctggtggtga catcggcagc accgctcccc gcatgggcag aagagaaggg cggtaaaatc
     1381 ctcaccgcgg gacgttgggg agcgatgaac gtagaagtga aggacggcaa gattgtttct
     1441 tcaacaggcg cgctggcgaa aaccataccg aattccttac agtctacggc ggcggatcag
     1501 gtacacacca cgacgcgtat tcagcatccg atggtgagaa aaagctatct cgataatcca
     1561 ctgcaaccga cgaaaggtcg tggtgaagat acctatgtac aggtgagctg ggaacaagca
     1621 ttaaaactga ttcatgagca acacgatcgc attcgcaaag ctaacggacc atcggctatt
     1681 tttgccggat catacggctg gcgctcttct ggcgtgttgc ataaagcgca aaccttactg
     1741 caacgttata tgaacctggc gggcggttat tccgggcata gcggcgatta ttcaaccggg
     1801 gcggcacagg tgatcatgcc gcatgtggtc ggttctgtag aggtgtatga acagcagacc
     1861 tcctggccgc tgattctgga aaacagccag gttgtggtgc gtgggggatg aacccgctga
     1921 ataccttaaa aattgcctgg agcagtaccg atgagcaagg gctggaatac ttccatcagc
     1981 taaaaaaatc tggcaaacca gtgattgcca ttgatcctat ccgctccgaa accatcgact
     2041 tctttggcga taatgccacc tggatcgcgc cgaatatggg caccgacgtg gcactgatgt
     2101 tagggattgc gcataccctg atgacacaag gcaaacacga taaagtgttc cttgaaaaat
     2161 acactaccgg gtatccgcag tttgaagagt atctgagagg taagagcgat aatacgccga
     2221 agagcgcggc ctgggcggcg gaaattactg gcgtaccgga agcgcagata gtcaaactcg
     2281 ctgaactgat ggcggcgaat cgcacaatgc tgatggcagg ctggggaatt cagcgccagc
     2341 aatatggtga acagaaacac tggatgctgg tgacgctggc ggcggtgtta gggcagattg
     2401 gtacaccggg cggcggtttt ggtttctctt atcattactc caatggtggc aacccgacac
     2461 gtgttggcgg cgtgctgccg gaaatgtctg cggcgattgc cggacaggcc agcgaggccg
     2521 ctgatgatgg cggaatgagg gcgattcctg ttgcccgtat cgtcgatgcg ctggaaaatc
     2581 ctggcggcaa atatcagcat aacggtaaag agcaaacgta tccgaatatc aaaatgatct
     2641 ggtgggcggg tggcggtaac tttacccatc accaggatac taatcgtctg attaaagcat
     2701 ggcagaaacc ggagatgatc gtcgtttctg aatgctactg gaccgcagct gctaaacatg
     2761 cagatatcgt attaccgatc accacatcgt ttgagcgcaa tgacttgacg atgaccgttg
     2821 attacagcaa ccagcatatt gtgccgatga agcaggctgt cgctccgcaa tttgaagcgc
     2881 gaaacgattt tgatgtgttt gccgatcttg cggaattact gaaacctggc ggaaaagaga
     2941 tctataccga aggtaaagat gaaatggcgt ggctgaaatt tttctatgat gccgctcaga
     3001 aaggtgcccg tgcgcaacgc gtgactatgc cgatgtttaa tgccttctgg cagcaaaata
     3061 aactgatcga aatgcgccgc agcgagaaga acgaacagta cgttcgttat gctgatttcc
     3121 gcgccgatcc gatgaaaaat gcgctgggta cgccaagcgg caaaattgag atttactcca
     3181 aaacgctgga aaaatttggc tataaggatt gcccggcaca cccaacctgg cttgcgcctg
     3241 atgagtggaa gggtaccgcc gacgagaagc agttgcagct tctgaccgca catccggcac
     3301 accgtttaca tagtcagctt aactatgcgg aactgcgtaa aaaatatgcg gttgcagatc
     3361 gtgaaccaat cactattcat accgaagatg ctgctcgctt tggtattgcg aatggtgatc
     3421 tggtgcgtgt gtggaacaaa cgtggtcaga ttctgacagg cgcggtggtg actgacggga
     3481 tcaaaaaagg cgtggtatgc gtgcatgaag gtgcatggcc agatctggaa aatggcttgt
     3541 gtaaaaacgg cagtgcgaac gtgttaacgg cggatatccc cagctcgcag ctggcaaatg
     3601 cctgtgccgg taactctgcg ctggtgtata tcgaaaaata tacgggtaac gcaccgaagt
     3661 gaacggcatt tgataagcct gctgttcagg cgtaagaagt gaatagcgcc cggaaacggg
     3721 cgctgaggtt atgcaaacgt aaaactgcct gatgcgctat gcttatcagg cctacatagt
     3781 ccctgcaata gattgaatta gcaagggttt gtaggccgga taaggcattc acgctgcatc
     3841 cggcagtaat accagacctt tacggcttgc gcgcaatcag caccgcgcgt ttaggcgcag
     3901 gataaccttc aactgtctta ctcggatcat gtgggtcgag gaaatcggac agagattcgg
     3961 taaccatcca ttcagtgcgt cgctgctctt cagtagtggt aacgcacaca tccacaacgc
     4021 ggatatctac aaaaccacac ttcttcagcc agtttttcag cgccagcgcg gaagggatga
     4081 aatagacatt acgcatttgc gcgtaacgat cgcctggtac cagcaccgtg ttctcatcgc
     4141 catcaataac cagcgtttcc agcaccagtt caccctcatt caccagttga tctttcaact
     4201 gccagagatg ctccagcggc gaacggcgat ggtaaagcac gcccatcgaa aagacggtat
     4261 caaaggcttt cagtgccgga agctgttcaa tacctaacgg taacaaatgt gcgcgctgat
     4321 cgttacccag tagtttacgc accgcttcaa actggcacag aaacagttgc gtagggtcga
     4381 tccccaccgc gaggtgcgcg cctgcgccaa tcatgcgcca catgtgataa ccgctgccgc
     4441 agccgacatc cagaatggtg cgtccggtta aatcagaaag atggggcaga acacgatccc
     4501 atttccagtc ggaacgccat tcggtatcga tgttgacgcc atacagtgag aacggccctt
     4561 tgcgccatgg catcaggttc cgtataagcg tttcaatgcg tttaatttgc ccggtgctca
     4621 gtggctcttc gctttcggcg gttacgctat gcaataaatc cagacgatac ggtttaattt
     4681 caggcagaaa ttccaccgcg ttggaccact gcttaaacag cccgtgctgc tgctcgcgct
     4741 gccagttagc aatctgcgcg ggcagcgttt cgagccagtg tgaaagatga tttttggcaa
     4801 tcagagaata aaagttacca aagtcgatca tgcagcgtcc tctgctttta atgccaccag
     4861 tgaaccaaag ttaaagcact ggaaccacag ctcgctatgc tcaaaaccgg ctttatgcag
     4921 gcgtgcttta tgggtttcca cggaatcggt cagcatcacg ttttccagca tgctgcgctt
     4981 ctggctgatc tccagttcgc tgtaaccgtt ggcacgttta aagtcgtggt gcatgttgaa
     5041 caacagttca ccaactttgg catcttcgaa actgaatttt tccgaaagca ccagcgcgcc
     5101 gcccgggttc agtccttgat aaattttatc cagtaatgcc tggcgctcgg aaggttccag
     5161 gaattgcagg gtaaaattca gcaccaccat cgatgcgttt tcaatggcga tatcgcgaat
     5221 atcaccttca atgacgtcta ctggcgtagg ggctttatag gcgtcaatat gacgacggca
     5281 gcgttcaatc atcgccgggg agttgtcgat ggcaataatt ttgcaattat catgatgaat
     5341 gttgcgacgc accgagagcg tcgccgcgcc cagagaacaa cccagatcgt aaacctgcgt
     5401 accaggttga acgaagcgct cggctaacat actaatcatg gaaataatat tggaatagcc
     5461 gggaacggaa cgctggatca tatccgggaa gacttcagct acccgttcat caaaggtcca
     5521 gtcgcccagt ctggcgatag gggcagaaaa tagcgtgtcg cggtgagaca taacgtaaaa
     5581 atccgggaaa aagaaagtgg cgtattgtgc gctaacgcag ggagaaaacc aactcccagg
     5641 gcatatacca aagattcgcc agcaccatca gcaacagcgc acaccaggtg gcgctcatgc
     5701 cggaacgtcg ccagcgaaac agacggtgat gaaaaccgta ataatgcatc agacgaccag
     5761 caagcaaaac gatgccgcaa atatgcacca tccaggtttc tgcgccattc atttccataa
     5821 acagcatcag cacaatcgcg atgggaatat attccaccgc gttaccatga atgcgaatag
     5881 cgctttgcag ttcgctaaaa ccaccgtcac cataggcaac gcggtactgc aaacgca
//
LOCUS       DAJTMK010000214         5914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTMK010000214 DAJTMK010000000
VERSION     DAJTMK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5914
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.218"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            46..171
                     /locus_tag="OO135_003733"
     CDS             46..171
                     /locus_tag="OO135_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5399402.1"
                     /translation="MIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            260..1720
                     /locus_tag="OO135_003734"
     CDS             260..1720
                     /locus_tag="OO135_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5399403.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHCAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1756..1959)
                     /gene="ydfZ"
                     /locus_tag="OO135_003735"
     CDS             complement(1756..1959)
                     /gene="ydfZ"
                     /locus_tag="OO135_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5399404.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2137..2823)
                     /gene="ydfH"
                     /locus_tag="OO135_003736"
     CDS             complement(2137..2823)
                     /gene="ydfH"
                     /locus_tag="OO135_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5399405.1"
                     /translation="MTVETQLDPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSHQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIEYAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQISDCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2912..3658)
                     /gene="ydfG"
                     /locus_tag="OO135_003737"
     CDS             complement(2912..3658)
                     /gene="ydfG"
                     /locus_tag="OO135_003737"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5399406.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3795..5840
                     /gene="dcp"
                     /locus_tag="OO135_003738"
     CDS             3795..5840
                     /gene="dcp"
                     /locus_tag="OO135_003738"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5399407.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKLDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLHWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1490 a         1387 c         1533 g         1504 t
ORIGIN      
        1 agttggagtc attacccgat cgccattatg atcatggcat attcagtgat tggtttaatc
       61 tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg
      121 gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aaccctcaat
      181 cccttctctt accggagagg ggatttttat tcatataaca aaacatatag cttgccatat
      241 ttaaatttaa ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt
      301 ttatgatcgt aataacctag ccccaagaat tgttcattta ggctttggtg catttcactg
      361 tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata
      421 ttatgaggtc aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa
      481 tctttatacc gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt
      541 taaaaaagcc ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga
      601 accgcaaatc gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc
      661 gaccggacag ttaatgctcg atcacccgat ggtcgctgcc gacgtgcaaa atccccacca
      721 gccgaaaaca gcaacagggg tgattgttga ggcgctggcc cgccgtaaag cggcaggact
      781 tcccgcattt accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga
      841 cgttgtcact tcatacgcga aagctgttga tgtaaaactg gcacaatgga tcgaagataa
      901 cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct
      961 ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgcg ggcgttgcct gtgaaccttt
     1021 ccgccagtgg gtaatagaag ataactttgt tgccggacgt ccagaatggg aaaaagcggg
     1081 agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg
     1141 cagtcattca ttcctggcgt atttgggtta tcttgccgga tatcagcaca ttaatgactg
     1201 catggaagat gaacattatc gttatgcggc gtatggcttg atgttgcagg aacaagcgcc
     1261 gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgaacgcta
     1321 tagcaatccg gcgttacgtc atcgaacctg gcagattgcg atggatggta gccagaaatt
     1381 gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct
     1441 gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa
     1501 tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc
     1561 cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtgatgattt
     1621 gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca
     1681 tggagcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg
     1741 gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc
     1801 ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc
     1861 agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc
     1921 agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat
     1981 ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt
     2041 ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa
     2101 aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct
     2161 gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc
     2221 acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg
     2281 taacagcatt tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt
     2341 cgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggaaatct gcgtcaggag
     2401 ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat
     2461 gcgttgctgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc
     2521 cgcccgacgc gccaccgcgt actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2581 ggccatggaa attttgttga cgtagctgcc acgctgtgga cgaatttgaa tcaggccgtt
     2641 ttccgccagt ttaataaagg cttcacgaac cggctggtgt gacacattga aacgaacaga
     2701 aacttctttt tccgacaacg gtgtgcctgg agcaatcagg caatggacaa tgtcgcgacg
     2761 aaggatacga taaatttgct gattgacagg ctgtgtggga tcaagttgcg tttcgacggt
     2821 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2881 aataacccgg cagttatgcc gggtataaaa attactgacg gtggacattc agtccggcat
     2941 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     3001 acacccacca aacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     3061 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     3121 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcaggtcc gtacgcagat
     3181 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcataag
     3241 gccagctacc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3301 ccggtaagac ggcgcgcgtc atatatacca ggcctttgtt gttggtatca atcatcgttt
     3361 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3421 ttaccaggat atcaatattg caccactcgg caggaagcga tgccagcatc tcttcaatag
     3481 cggcgcggtt gcgaacgtcc agttgggcga tgtacagatt atctcccagt tcgtctttta
     3541 actcctgcaa ccgttcctgg cgacggccag tggcgataac tttatgccct tgttgaataa
     3601 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3661 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3721 atactggttt gtaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3781 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctaccgtatc
     3841 tggctcccca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3901 tgcagcaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     3961 acaatactat tctggcactg gaacaaagcg gagaattact tacccgcgtt accagcgtct
     4021 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     4081 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     4141 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4201 tggaggtgat tcatcaacgt tttgtccttg ccggagccaa acttgcgcaa gctgataaag
     4261 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcgat
     4321 tactggcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4381 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4441 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgag atgcgcgatc
     4501 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4561 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4621 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4681 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgct agcgatgaat
     4741 tagcctccat acaggcggtt atcgataagc agcaaggcgg gtttagcgcg cagccgtggg
     4801 actgggcatt ttatgccgaa caggtacggc gggagaaatt ggatcttgat gaggcgcagc
     4861 tcaagccata ttttgaatta aacacggtgt tgaatgaagg tgtattctgg accgcgaatc
     4921 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc tgtctaccat cctgacgttc
     4981 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     5041 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     5101 ttaatgaaac gcatccggta atttataacg tttgcaatta tcagaaaccc gctgccggtg
     5161 agcctgcgtt gttacactgg gatgatgtca taaccttatt ccatgaattt ggtcatacgc
     5221 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5281 attttgttga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5341 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5401 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5461 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     5521 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5581 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5641 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5701 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5761 tggaacgcct gtatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5821 atcgtggctt gaatatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5881 ctggttatag cagtaattgc agcagctgga cgag
//
LOCUS       DAJTMK010000215         5909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTMK010000215 DAJTMK010000000
VERSION     DAJTMK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.219"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..228
                     /gene="fadM"
                     /locus_tag="OO135_003739"
     CDS             <1..228
                     /gene="fadM"
                     /locus_tag="OO135_003739"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5399408.1"
                     /translation="INYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVAD
                     ALITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(280..975)
                     /gene="queC"
                     /locus_tag="OO135_003740"
     CDS             complement(280..975)
                     /gene="queC"
                     /locus_tag="OO135_003740"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5399409.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1040..2740)
                     /locus_tag="OO135_003741"
     CDS             complement(1040..2740)
                     /locus_tag="OO135_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR5399410.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2840..3658
                     /gene="cof"
                     /locus_tag="OO135_003742"
     CDS             2840..3658
                     /gene="cof"
                     /locus_tag="OO135_003742"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5399411.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            3811..4269
                     /gene="decR"
                     /locus_tag="OO135_003743"
     CDS             3811..4269
                     /gene="decR"
                     /locus_tag="OO135_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5399412.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4299..>5909
                     /locus_tag="OO135_003744"
     CDS             4299..>5909
                     /locus_tag="OO135_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5399413.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQFSWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEA"
BASE COUNT         1356 a         1466 c         1593 g         1494 t
ORIGIN      
        1 attaactatc gtcgcccagc ggtattaagt gacctgttaa ctattaccag tcagttgcag
       61 caattaaacg gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag
      121 gtggtagcgg atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta
      181 gctctggaag gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt
      241 gccggatgct caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg
      301 cttcatcgct gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg
      361 taaattacat gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa
      421 cgtttcgtta cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc
      481 tttatcaatc cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac
      541 ggcatggttt agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt
      601 ttcacagacg ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag
      661 cgtcaggaac aaaatattac gccctgggac aaacgtattc gggatgccat cggcttcagg
      721 ttcataatca ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag
      781 cagcgttaca tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc
      841 cacgtcgatt tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc
      901 atattgttgt aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac
      961 gacagcacgt ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg
     1021 attaacccgg accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa
     1081 accagccgcg cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt
     1141 gatagttgaa cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca
     1201 gggcattaaa tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca
     1261 gatgtgcata tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt
     1321 gctccagagt atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt
     1381 gtgccagtag ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac
     1441 agccttccgc tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta
     1501 ggtgataaac cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg
     1561 gaatagtcca gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta
     1621 acgtttgtaa taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg
     1681 agagtcgggg acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga
     1741 cctggctgac ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac
     1801 aactggttcc caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa
     1861 gcagcggatg acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga
     1921 attgtgttaa gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc
     1981 tgcaatagct cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca
     2041 gacactgcgg atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg
     2101 gcagttgtaa cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt
     2161 tatgccagtg tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt
     2221 cccagtgatg cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc
     2281 cggaaaatat ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta
     2341 gcggttcgag cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc
     2401 cgcccataaa cggctgtaac agagtgcgta gctcacctgg ggccagttgc gccagctcca
     2461 gcacatcttg ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt
     2521 ccggcgtgac cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact
     2581 ccagccatcc cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa
     2641 aacagcgttc ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc
     2701 acagacgttg atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta
     2761 tttaactatt cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg
     2821 tcacagagtg gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt
     2881 gatgcctgac catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg
     2941 cgacattacc ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg
     3001 ggcgctatcg ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga
     3061 aggtgaactt ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca
     3121 gcaatgggat acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa
     3181 agagatccct gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga
     3241 tgtcaaaaaa atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga
     3301 tcttacacgc ttgcagatcc agctatacga agcattgggc gagcgtgcac atttgtgttt
     3361 ttccgccacg gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac
     3421 ggtgctgacc caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat
     3481 gaacgatcgc gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc
     3541 gcaactgcgc gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt
     3601 ctctcactat ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataatg
     3661 agatcccttc cagcaccggg caattgcccg gttttttttg cgttgaattt gtcattttgt
     3721 gccgtggtgt ttaaaccgca cagaataaat tgtcgtgatt tcacctttaa aatagaatta
     3781 aaagagaaaa aaattctctg tggaagggct atgttagata aaattgaccg taagctgctg
     3841 gccttactgc agcaggattg caccctctct ttgcaggcac tggctgaagc cgttaatctg
     3901 acaaccaccc cttgctggaa gcgcctgaaa cggctggagg acgacggtat ccttatcggc
     3961 aaagtcgccc tgctggatcc ggaaaaaata ggcctcggcc tgaccgcctt tgtgctgata
     4021 aaaacgcaac atcacagcag cgaatggtat tgccgctttg tcacggtggt taccgaaatg
     4081 ccagaagtgc tggggttctg gcgcatggct ggtgaatacg attatctgat gcgcgtccag
     4141 gttgccgaca tgaaacgcta cgacgagttt tataagcgtc tggtaaacag cgtgccgggg
     4201 ctgtcggacg tcacttccag cttcgcgatg gaacagatta aatacaccac ttctttaccc
     4261 atcgaataaa tatccagaat caggtcagga cacaacgcgt gcgattattt gctcaattaa
     4321 gctggtattt ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg cttgtcatta
     4381 tcgcgatgct gcaactggtt ccgccaaaag tggttggtat tgttgtcgat ggcgtgacag
     4441 aacaacactt tactaccggg cagatcctga tgtggatcgc caccatggtg ctgattgccg
     4501 ttgtggttta tttcctgcgt tacgtctggc gggtattgct gtttggtgcg tcttatcaac
     4561 tggctgttga actgcgtgaa gattattacc gtcagctaag ccggcagcat cctgagtttt
     4621 acctgcgtca tcgcacgggt gacctcatgg ctcgtgcgac caatgacgtc gatcgcgtcg
     4681 tgtttgcagc cggagaaggg gtgctgacgc tggtggattc actggtgatg ggctgcgctg
     4741 tgttgattat gatgtctacg caatttagct ggcagttgac cttatttgcc ctgttgccga
     4801 tgccagtgat ggcgatcatg atcaagcgca acggcgatgc cttgcatgaa cgctttaagc
     4861 tggcacaggc ggcgttttct agtcttaatg accgcaccca ggaaagcctc accagtatcc
     4921 gcatgatcaa agcctttggc ctggaagatc gccagtcggc gttatttgcc gcggatgccg
     4981 aagataccgg caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc gacccgacca
     5041 tctatatcgc gattggtatg gcgaacttgc tggcgattgg cggcggtagt tggatggtgg
     5101 tgcagggcag tttaacgctg ggccagctca ccagttttat gatgtattta ggtctgatga
     5161 tttggccaat gctggcgctg gcatggatgt ttaacattgt ggaacgtggt agtgctgcat
     5221 acagccgtat tcgcgcgatg ctggcggaag cgccggtggt gaacgatggt agcgaacccg
     5281 tgccggaagg gcgtggcgaa ctggatgtaa atattcacca gttcacgtat ccgcagactg
     5341 accatcctgc gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg ctgggtatct
     5401 gcgggccgac tggttccggc aaaagtaccc tgttgtcgct cattcagcgt catttcgacg
     5461 tcagcgaggg ggatattcgc tttcatgata ttcctctgac gaagttacaa ctcgatagct
     5521 ggcgtagccg cctggcggta gttagccaga caccgttcct tttttccgac accgtagcga
     5581 acaacatcgc gttgggttgc ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt
     5641 tagccagcgt acatgacgat attttgcgtc taccgcaagg ttacgataca gaagtgggcg
     5701 agcgcggcgt gatgctttcc ggtgggcaaa aacagcgtat ctccattgct cgagcgttat
     5761 tagtcaacgc ggaaatcctc atccttgatg atgcgctttc ggcggtggac ggacgcacag
     5821 agcaccagat cctgcataac ctgcgtcagt ggggacaggg aagaacggta atcatcagtg
     5881 cccatcgcct ttcggcactg acggaagcc
//
LOCUS       DAJTMK010000216         5817 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTMK010000216 DAJTMK010000000
VERSION     DAJTMK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.220"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            151..267
                     /locus_tag="OO135_003745"
     CDS             151..267
                     /locus_tag="OO135_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5399414.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            631..1473
                     /locus_tag="OO135_003746"
     CDS             631..1473
                     /locus_tag="OO135_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5399415.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1476..2564
                     /gene="capU"
                     /locus_tag="OO135_003747"
     CDS             1476..2564
                     /gene="capU"
                     /locus_tag="OO135_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5399416.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2569..3519
                     /gene="virK"
                     /locus_tag="OO135_003748"
     CDS             2569..3519
                     /gene="virK"
                     /locus_tag="OO135_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5399417.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQKL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3584..4528
                     /gene="lpxM"
                     /locus_tag="OO135_003749"
                     /gene_synonym="msbB"
     CDS             3584..4528
                     /gene="lpxM"
                     /locus_tag="OO135_003749"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5399418.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4682..5712)
                     /locus_tag="OO135_003750"
                     /pseudo
     CDS             complement(4682..5712)
                     /locus_tag="OO135_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1578 a         1185 c         1363 g         1691 t
ORIGIN      
        1 agttatcggt ggcaatagcc tgtgcaaatt ttttataacc cgggatcgtg tcaggattca
       61 gagtatgata aagacagatt ttcatggctg ccggtttccg tcgtgcaaaa aacatgctga
      121 ttttacgaag gaatgcctta ctgaacaacc ttgcccggaa taccccccga aatcagatgg
      181 aacaatcaga aaaatgctct gcagttatgg aataaagaag gtggggtggt acgccgggag
      241 ggcgatccgc tggaaatggc gcagtagctg caggcctgtc atgatgccgg aatagaagtt
      301 cgtgtacagg ttaatgaaag cgtcaccccg taaataaact ctgcgctgaa attccttttt
      361 ttcattggag cacctgtgtt gttctgaggt gaacatatca cctctgttca ggtagccaaa
      421 ttcagtgtgc cacctcaagg ttcttagtga gtcgttttta aaagatatga taatatatca
      481 ttgtttatga attgttgatg tttaatttag taaaatatat caagtatgcc atgggtttgc
      541 tatgttattc atccagtatc tggaaataaa atgcaccagc tttattgggt tttccttttg
      601 tctctgctta ctaggtgcca gaaactttaa atgttaaacg aagggggcat tttgttcaaa
      661 gccaatcatg ttccggtatt aatgtatcat catgtcagtc attgccccgg actggttaca
      721 ctttctcccg tgacattccg gaagcaaatg aaatggttgg ctgaaaacaa ctggaaaaca
      781 ctgtcatcag atgaactgga gtttttttat cggggcggaa aactcccccg gaaaagcgtt
      841 atgttgacat ttgatgatgg ataccttgac aactggtttc aggtatatcc tttattaaaa
      901 gagtttaatc tcaaagcaca tattttttta attaccggtt ttattgggaa tggtccggtt
      961 cgtcactctc ctggaaagga atattctcat cgcgattgcg aacatcaaat agcgacaggg
     1021 aatgctgata atgttatgct tcgctggtct gaagtaaacg aaatgttgca atctggtctg
     1081 gtcgaatttc atgttcatac gcatactcat acacgctggg ataaaaagtt cagttcacga
     1141 gaagaacagt gtaaacatct tcggcaggac ttgttgagcg ggagagaata tttgaaagaa
     1201 atgacaggaa aatgcagtaa acatttgtgt tggccggaag gttattataa caaggattat
     1261 attcaggtcg ctgaagaact tggattctat tatttatata caacagaaag aagaatgaat
     1321 gctcctgcta aaggaactac acggatagga agaataagca ccaaagaacg ggaaagttgc
     1381 gcatggctga aaagacgact tttttattat acaacaccgt ttttttcatc gttgctggcc
     1441 tttcataagg ggccaagatt accggatgat taataatgaa tatactattt acggaatcat
     1501 caccgaatat aggtggtcag gaattacagg ctgttgctca aatgaaggcc ctgaagaaaa
     1561 tggggcattc agttctgctt gtctgcaggg aaaacagcaa aattgctttt gaagccagta
     1621 aattgggaat tgatatcaca ttcgcgttat ttcgaaacag tcttcacatc cctactgcat
     1681 ggagattact cggaatagtt catggttttc agcccaatgc aatcgtttgt cacagtgggc
     1741 atgatagcaa tattgttggt ttagtacggt tattcacttg gaaacatcca ttcagaatta
     1801 tcaggcaaaa gacatatttg acacgaaaaa caaaagtttt ttcaataaat catttttgcg
     1861 atgaggtgat tgttcccgga acaagtatga agacacatct ggagcaggaa ggatgtcgaa
     1921 cccgggttac tgttgtgcct ccaggctttg atttccagaa attatacgtt gattcccgaa
     1981 acagtttgcc tccaaatgtt ctttcttggc tggcgtcccg aaggggatgc cccgttattg
     2041 ctcaggtagg aatgttgcgc ccggaaaaag ggcacgaatt tatgttgaat ttactgttcc
     2101 atttaaaaat gaatggacga cagttctgtt ggttgattgt ggggtctggt tcgcctgaac
     2161 tgcgggagca tttacagtat cagattgaca gtatgggcat gcatgatgat gtttttattg
     2221 ctgacaatgt ttttcctgcc gcccccgtat atcgggttgc cagtctggtg gttctgcctt
     2281 cagaaaacga atcgtttggt atggtactgg cagaagcatc ggcattttct gtgcctgtac
     2341 tggccagtca gattggtgga atccctgatg ttattcagaa caaccagacc gggacattgt
     2401 taccagcagg taataagcac gcatggatgt gcgccctgaa tgattttttt aatgaccctg
     2461 ggcgttttta tcagatggct cgccaggcaa aacaggatat agaagagcgg tttgatatta
     2521 ataaaactgc gttaaaaata ctcacattag cgaagcacaa gtaacaatat gttttctgta
     2581 agtaacttat catttatcgg tttccttaaa aggattgttt tttcctcaga ttcactcccg
     2641 gggaagtggg aacacagaaa attccggttc atgtacattt tgcgatgtgc tataaatccg
     2701 gttgccagta ttcgatatta ttacgaactg cgttccttgc cgtgcattga ggatattctg
     2761 gcaatacacc ccacgttgcc agcaagaatt catcgccctt atttgcataa aggggggcgc
     2821 gcctggaccc gtggacaata tattcttgag cattatcgct ttgtgcaaaa attgccagaa
     2881 aaatattcta aatttttgtt tccacaaaaa tcggtatcat tagtccaatt catcgggaaa
     2941 gatggagaga attttgatat ccagtgttct cccagtggtt ttgaccggga aggagagttg
     3001 atgttgtcat tgttttacaa taaaacagtt attacccggt taacattttc agtcattctg
     3061 actcaaaatg gacacattgc ttttattggc ggattacagg gagcccctaa aaataccgga
     3121 cctgatgtta ttcggtgcgc aacccgggcc tgttatggga ttttcccaaa acggattatt
     3181 tttgaagcat tttgtgcgtt gatgaaagct tgcaatatct ctgagtgtct ggcagtcagt
     3241 gagcacagtc atgttttccg acaattgaga tactggtatc agaaacgcaa aacctttgtt
     3301 gcggtctata gtgatttctg ggagtctgta gcaggaaaaa cctgtggtga ctggtacaga
     3361 ttaccaaccc aggtgattcg aaagccgcta agcgatatag ccagtaaaaa acgctctgag
     3421 taccgaaaac gatacgcttt gctggattat atccatgaaa ctactatccg ctctttggat
     3481 gcatatcctg tacactcaga acatcaggat ttaaattaag gtttgatgta ttctctgaat
     3541 aaaacagtaa tgataatatt ggtagaggca ttcgcactaa ataatgaaaa aatataaatc
     3601 tgagtttatt cctgaattta agaaaaatta tctttcccct gtttactggt ctacatggtt
     3661 ccttttggga atgattgcag gtatttcaat gtttccccct tcattcagag atcctgtctt
     3721 ggcaaaaata gggcgttggg cgggcagatt gagcaaaaaa gctcgtcgca gggcgacgat
     3781 taatttatcg ctttgtttcc cagaaaagag tgatacagaa cgggaaataa ttgtcgacaa
     3841 aatgtttgcc acagcattgc aatccatagt gatgatggca gaactggcaa ttcgtggtcc
     3901 ggaaaagttc cagaaacgag tgttctggaa aggcctcgaa attcttgagg aaattcgata
     3961 caataacaga aatgtgattt ttctggttcc ccatggctgg agcgtggata ttcctgcaat
     4021 gttgctggca gctcaggggg aaaaaatggc cgccatgttt catcagcaac gaaatccagt
     4081 gattgattat gtctggaatt ctgtacggcg taaattcggg gggcgcttac attcccgtga
     4141 ggatgggata aaaccattta ttcagtcagt acgccaggga tactgggggt attaccttcc
     4201 agatcaggat catggtcctg aatacagtga atttgctgat ttttttgcga cctataaagc
     4261 gacattacca attattggac gtctgatgaa catcagtcag gctatgatta taccgctttt
     4321 cccggtttat gatgaaaaaa aacatttcct gactattgaa gttcggccac caatggatgc
     4381 atgcattgcc agcgcggaca ataaaatgat tgcccgacaa atgaacaaaa cagtggaaat
     4441 tttggtgggg tcacatccgg aacagtatat ctgggtttta aaattgttaa aaacgcgcaa
     4501 atcaaacgaa gcggacccgt acccttgaaa ttataaagta caggtatttc cactagttgt
     4561 ttcttacagg ttaccaatcg aaacacatcc cccttccgtg tgttgcccgt ttggcacgga
     4621 ggactgtgta gatgcagttc gttaaattta atttatgcct aaatagctgc gcttaatacc
     4681 gctacacttt tgccagtcca tgtttgcctc cggggaatgg gctgacggtt tccataaaat
     4741 ggcgaacttt tttcaacagt tgccacattg agcggcactg atgattacgc gttattgtgt
     4801 cgtgaagtgc ctgccatagc cgttcagcat gattcaccca tggcgagtaa accggctgat
     4861 aaatgagcct gaacttcggg ttctccttca gccagctctg tgtttcccgg cttttgtgga
     4921 taatgtagtt gtccacgatc agcgtgatgg ttttcgcccg acggtatgtc gctttaagcc
     4981 gcttcagcag gctgatgaac agcgccgaac ttttgctgtt gccgcccaca tagctgactt
     5041 tacctgtccc gctgtgcagc gctccggcca gataatattt ttcattctgt cccggcgtga
     5101 ccacccgttt ttgctgtccg cgcagttgcc agtccgcacc gattttggga tgaagatgga
     5161 tatccacttc atcttcataa aagaccggat gctctgcgct gcattcgtcc agtgctttat
     5221 ggattgctgc catcttttca tctttatgcg ggtcacggat acgcagagtt ggcgcagccc
     5281 ttcgccacac aatccccgca gacggcaacc agcggcgaac ggttccggca tttaactggc
     5341 aaccggttat ctcattgatt tttattgcca gcagttctgt actccagcgt gaacgctggt
     5401 agccaaagtc gccggagaat gttttaccag ctcacgtaac agtgtgcaga tatgctcaaa
     5461 cggccagcga cgggcacgcc cggcaggtaa tgatttcagt ccctcaacac ccgactgcgt
     5521 gaaccagtta atccagcgtc caacagagga acgggcgcag cagagcgttc tggcaacgtc
     5581 gctgacacgg tcgccccggt gcagcatcag catggcagtc agtctgcggg cataattttt
     5641 atcgtgtgtt ttatggatgg ctttctgcat caggcgtcgt tcgtcacggg aaattggtgc
     5701 tatgatcggc attgctcagt ccggttggtg atttgttttg atttggcgat tgatcagatc
     5761 gcacaatccg ggctaagttc cctcaaaggg atctactatt ccgcgcagct atttagg
//
LOCUS       DAJTMK010000217         5798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTMK010000217 DAJTMK010000000
VERSION     DAJTMK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.221"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..122
                     /locus_tag="OO135_003751"
     CDS             <1..122
                     /locus_tag="OO135_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399419.1"
                     /translation="GTVNKSKVSLGLSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="OO135_003752"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="OO135_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5399420.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3181
                     /gene="safA"
                     /locus_tag="OO135_003753"
                     /pseudo
     CDS             2983..3181
                     /gene="safA"
                     /locus_tag="OO135_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
     gene            3256..4017
                     /gene="ydeO"
                     /locus_tag="OO135_003754"
     CDS             3256..4017
                     /gene="ydeO"
                     /locus_tag="OO135_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5399421.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4419..>5798
                     /locus_tag="OO135_003755"
     CDS             4419..>5798
                     /locus_tag="OO135_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5399422.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHK"
BASE COUNT         1677 a         1261 c         1262 g         1598 t
ORIGIN      
        1 tagggacagt aaataaaagc aaagtatctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt tgcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgatcttag tctatgcgct ccattcacga aaattgcgcg gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcgggacgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg accagtgtgg
     1021 gtttggcagc gagtatcggc gtgggtaaag ggactgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccacccccgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcgtac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgca
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatc
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgcatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt tttttcggca attgatcagc tgggtatttc
     3121 agaaatgggt cgcattgcaa gagatcttac acatttcatt atcaatagtt tgcaagactg
     3181 aaaataaaca gcaaattata aatatgaaat gttaaaaaag tatcgataaa aactttattg
     3241 ttttaaggag ataaaatgtc gctcgtttgt tctgttatat ttattcatca tgccttcaac
     3301 gctaacattt tagataaaga ttacgccttc tctgacggcg agatcctgat ggtagataac
     3361 gctgttcgta cgcattttga accttatgag cggcatttta aagagatcgg atttactgaa
     3421 aataccatta aaaaatatct acaatgcact aacatccaga cagtgacggt gcctgttcct
     3481 gcgaagtttt tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat
     3541 ctcaactcgg aagaacgcaa tcatcataat ttctcagaac ttttgctttt ttcttgcctg
     3601 tctatttttg ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt
     3661 tccggaaaag tgagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat
     3721 atttgtgact gcctgtatat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa
     3781 acgacattct cacagattct attagatgcc agaatgcagc acgcaaaaaa tttgatacgc
     3841 gtagaaggtt cagtcaataa aattgccgaa caatgtggtt atgccagtac ttcttatttt
     3901 atttatgcgt tccgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt
     3961 tgtcaaagtc acacgggtat gaatacgggc aacacgatga gtgctttagc tatttgatta
     4021 tttgctaacg agtagtcaac cacgcacgct acgtaagaat tatatggggc agccattccc
     4081 tgccccgcgt tgtttttagg cgatatattt attgaaataa ataagtgaca tccatcacat
     4141 atttatgcac ttgcataacc tgttgcatga ttatttatga tctcaattca gcattttgtc
     4201 agtaaaatgc aataatttat taaatatcaa taaattagtt gtttatcggc gagaaattac
     4261 ttaatagaac agaaagtaat gtcaacgctt tatggactgt tatttccctt tttttagcta
     4321 aatctgctat ctctttatgt gactaacttc acttacatcc acttatttct cttcgtaaaa
     4381 ttacttcgga attaagtaca ataagaagag gaacatttat gaagtctgca ttaaagaaaa
     4441 gtgtcgtaag tacctcgata tctttgatac tggcatctgg tatggctgca tttgctgctc
     4501 atgcggcaga tgatgtaaag ctgaaagcaa ccaaaacaaa cgttgctttc tcagacttta
     4561 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
     4621 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
     4681 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
     4741 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
     4801 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
     4861 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
     4921 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
     4981 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
     5041 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
     5101 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
     5161 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
     5221 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctgc
     5281 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
     5341 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
     5401 aactgaagaa aaacggacag tatgataata ctattattct ctttacctcc gataatggtg
     5461 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     5521 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     5581 attatgacaa gctgatttcc gcaatggatt tctacccgac agctcttgat gcagccgata
     5641 tcagcattcc aaaagacctt aagctggatg gcgtttcctt gctgccctgg ttgcaagata
     5701 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     5761 acgaggaaaa tattccattc tgggataatt accacaaa
//
LOCUS       DAJTMK010000218         5798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTMK010000218 DAJTMK010000000
VERSION     DAJTMK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.222"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(613..756)
                     /locus_tag="OO135_003756"
                     /pseudo
     CDS             complement(613..756)
                     /locus_tag="OO135_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(1090..1356)
                     /locus_tag="OO135_003757"
     CDS             complement(1090..1356)
                     /locus_tag="OO135_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399423.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            1497..1703
                     /locus_tag="OO135_003758"
     CDS             1497..1703
                     /locus_tag="OO135_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5399424.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            1798..2400
                     /locus_tag="OO135_003759"
     CDS             1798..2400
                     /locus_tag="OO135_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399425.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            3600..4517
                     /locus_tag="OO135_003760"
     CDS             3600..4517
                     /locus_tag="OO135_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399426.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            4602..5474
                     /locus_tag="OO135_003761"
     CDS             4602..5474
                     /locus_tag="OO135_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5399427.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         1437 a         1468 c         1368 g         1525 t
ORIGIN      
        1 ttgaattttc agcgtcgttt tttaatgtaa aaaaccggtt acgggtaagg ctgtgaggag
       61 ggagtgatgg tgatacgcaa aaagaagtgc agggactgtg gaaacgcgat tactcataat
      121 acagtgtgtt gcccgtattg tggttctgtc gaccccttcg gctactaccg gaatacagac
      181 cgtatcgtta ccatactgct ggcgctgatt attgtggtcc tgctgactac tgtcagcgtc
      241 agtgtgtgta tactgtgtag ctggtgagat aacggttcat gtatcccctg agctgacagt
      301 gtaccgtgta tattaagcgt aaccgacagg ttctccttca taccatatta aattttccag
      361 ggattgccgc tgccttattt cgtcccatac aggaaaactc atctgggttt ctgacaaata
      421 cgcgaaactc acagcccctc aatatcaacc acacgttgta agccggtaat acctgaaaat
      481 cagcctatta ccgcccacgc ctccggcacc attccctttt gatttcgaat tctttgttgt
      541 cgctataccg cttaatgttc ccggtcctcc ggtgcagata acaccggagt catcgtagcg
      601 ctgcttcaat attcaggtga cgtatttcca gacggaagcc ggcgcgcgat cgtaaagttt
      661 gttcattgtc agttctgcca gccggttgtc aacggcagag ttaaaggctt ccagctccgc
      721 aagggtaagt ttataacgcg tgtgggaatt cacttttccc gcgtctcctg acgtattacg
      781 ggctgccact tacctggtgt cccgcaatac agccttagtg ttccgttatc ggggatccgg
      841 acgcaccgca acaaccgggg aagtatttct ggtttagagc agtgtcaaga gggatttctc
      901 agcccctcac tcaccggctc agtctcaggg caggagcatt aatacgctca gttcatgtct
      961 tatacaggaa tgggcacagg gggagctggc gtgtcactgc tgattttacg catcagcgtt
     1021 catgtatctg cactctgata catgaacgat tcacagcgct ctgttaccgg agagatttac
     1081 tctcttagct tactctggca aatcctttac gttactctct gatgacttct tatatttata
     1141 ataccaatca gacagcctta tcccatcggt gatatcagtc tcctcctcgc caggaacggg
     1201 ctgactcact gtaactacct ccgaaaacaa ctctgcaatt atcagaacgc ctgcttctct
     1261 ccctgtcctc acgaaaacta tcccctcttt atcgcgcgtg cgtgcggaag catcttttcg
     1321 caacaaccac ccggtatacc ggtacggctc agccaccgca acctccccgt ttatctcctg
     1381 gcggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg gtgaaggtgg
     1441 cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac attctcatgg
     1501 ctaccccagt ttcactgatg gatgaccaga tggtcgacat ggcatttatc actcaactga
     1561 ccggcctgac agataagtgg ttttacaggc tcatcaggga tggggccttt ccggctccca
     1621 tcaagctggg ccgcagctcc cgctggctga aaagtgaagt ggaagcctgg ctacaggcgc
     1681 gtattgcaca gccccgcccg taatttctga ccgttatccg ttcacccgca gcagacgtaa
     1741 tccccggcct gccggcggca ttctgctgcc tgttatatcc ccgtgaggaa tatgaaaatg
     1801 aaacaacagt atcagacccg ctacgaatgg ctccacgaaa gctaccagaa atggctgacc
     1861 ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcta ctacttccat
     1921 aatctgacgc ccggttgggt gtcattcaat ggtgaaaaac cggagattgc catcgtaccc
     1981 cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc cctggatgat
     2041 gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc gatgctggaa
     2101 ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa taaactgatc
     2161 agcctcttcc gtcagtttgg tggcacggag ttgcgcctca aactggtctg gctttgctgg
     2221 cttgatttaa tgaccggaaa cagccttgaa gactggaaag agaacctgaa acggaaatca
     2281 gaaaaagagc tggaggaatg gatcatcaac cgtcagaggc agagcgcagc actgacggat
     2341 ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgatag ccgcaactga
     2401 tgccggttgc ttccggaagg tgtgacaaaa tacggaaatg atgatgctcc cggcccccat
     2461 gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg tcttaagggc
     2521 atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg atgttcttta
     2581 cagcccgata cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt attcaccaga
     2641 aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg acagcgatcc
     2701 cggcactgtg gaagagactc actcagcctg caggatattc tttccggcat ccgggaatgt
     2761 ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc atcataaata
     2821 atttatgcag atacagttaa ccgttacagt agccggtttg ttttaccggg aaataatatc
     2881 cgtcagactg ataccggaac atatctgaaa cacgttatga tgttttgtta tcagaaatgt
     2941 tgtatattct tatccgtggc gacagtttaa tgccctctgg tatcacctga taatgtatat
     3001 cctgcgatga ttcgttatat ttacgggaca cgcctctttt cattgtgctc cttttcagac
     3061 ttcattttcc gtaagtgttc cccggcataa aggaggtacc tttctggcgt ttataactct
     3121 ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg tattaacaga
     3181 cgatatttca gtgtccataa tttttctgtc cggttaaata acgtcagtct gctgccctct
     3241 gactgtaatc atcaaagccg gaatgcatac cgggttacaa cgtaaaaaaa gtgatgctgg
     3301 cccccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg tctgcagtct
     3361 ccactcttcc atcctcttaa gctacgtcgg ccacatgagt gcacttccat gataacaggt
     3421 attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc ctgtcagaat
     3481 gactatacag cgcgcagatt gcctttttat tgaccatctt aatataccgt tataaacttc
     3541 tttttatcat attattatca ccttattatg tttaattttt aagcagtcgg ggaaagagga
     3601 tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg tattactctt
     3661 attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat cccccggaac
     3721 gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt tcagattttg
     3781 aacttgaaca aataatttct ggtatgcgtc tgaaggccct ggccttcctg gtccccctgg
     3841 aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg tatctgttga
     3901 tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa aagaataaac
     3961 tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca tcgacacagt
     4021 tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg gatgagtgga
     4081 taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct ttcagttacc
     4141 tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag ggaaaaaata
     4201 ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac gcaattcacc
     4261 tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata aaaaatttca
     4321 gaaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag gaagataaag
     4381 tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa ttatcgaggg
     4441 tcagaagact cagtaccggc gaaatgctgc atgacctgat tgttgaagag tataaaaggt
     4501 accgacattc cagataattt cagacatata ttatctccgt gagtcccccg ccacctttcc
     4561 ggtgtgcggg ggttttgtct tttttcaccg ggaatacatg tatgaatccg tctgatgcca
     4621 ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc actttcccgt cacatcctgg
     4681 aacatctgcg caaactcacc agtcacgaac ccgtgattgg cattatgggt aaaagcgggg
     4741 ccggtaaatc ctcactctgt aatgcactgt ttcaggggga ggtcaccccg gtcagtgatg
     4801 ttcacgccgg cacccgggaa gtgcagcgtt tccgtctgag tggccatggt cacagcatgg
     4861 ttatcactga cctgcccggg gtgggcgaga gccgggacag agatgcagag tatgaagccc
     4921 tgtaccgtga cattctgcct gaacttgacc tggtactgtg gctgattaaa gccgatgacc
     4981 gtgccctgtc tgtggatgag tatttctggc gacacatcct gcaccgggga catcagcagg
     5041 tgctgtttgt ggtgacgcag gccgacaaaa cggagccctg ccatgaatgg gatatggccg
     5101 gtattcagcc ttctcccgca caggcacaga atattcgcga aaaaacggag gcggtattcc
     5161 gtctgttccg gcccgtacat ccggttgttg ccgtatcggc ccgcaccggc tgggaactgg
     5221 atacgctggt cagtgcgcta atgacagcac ttcccgacca tgccgccagt cccctgatga
     5281 cccgcctgca ggacgagctg cgcacggaat ctgtccgggg tcaggcccgt gaacagttta
     5341 ccggtgcggt ggaccggata tttgacacgg cggagagcgt ctgtgttgcc tctgttgccc
     5401 gtacggttct gcgtgccgtc cgtgacacgg tggtctctgt tgcccgcgct gtatggaact
     5461 ggattttctt ctgaacagtc tgccagggaa atgcctgata acattacgtt aaccaactga
     5521 aaacatgaca caaacccctc atactcctcc tgtttctctt gcgtgaccgc cttgtcgtta
     5581 atagaataaa actatcgata aaacagatat cgatcgttgc tatcgatcgt ttatagtggt
     5641 taatggtctg cgaaaaggca gctgaatctc ttcatcatgc agaacggaat tgcacacaac
     5701 ggactgatac ttctctggcg gatgagagag gggaagcatt tatgtccggg aaaaaccact
     5761 ttatctgacc atgctgtttt gtacctgccg gtatccac
//
LOCUS       DAJTMK010000219         5756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTMK010000219 DAJTMK010000000
VERSION     DAJTMK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.223"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..752
                     /gene="tam"
                     /locus_tag="OO135_003762"
     CDS             <1..752
                     /gene="tam"
                     /locus_tag="OO135_003762"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5399428.1"
                     /translation="WNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTA
                     LLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQW
                     LPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVH
                     AYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRY
                     HQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(756..1670)
                     /locus_tag="OO135_003763"
     CDS             complement(756..1670)
                     /locus_tag="OO135_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5399429.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSEILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(1867..2775)
                     /locus_tag="OO135_003764"
     CDS             complement(1867..2775)
                     /locus_tag="OO135_003764"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: protein motif:HMM:NF016846.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5399430.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGNVTSVTLPKLTRNTDSYRGGEVA
                     KLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVA
                     ILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIDEEIIQVLDLPRDELESFAS
                     AVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFALTALIAF
                     YRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTPELVAIVLAEKDHWEQDLTQVP
                     GLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(2775..3959)
                     /locus_tag="OO135_003765"
     CDS             complement(2775..3959)
                     /locus_tag="OO135_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR5399431.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYMGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWNKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGVRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            4117..5226
                     /locus_tag="OO135_003766"
                     /pseudo
     CDS             4117..5226
                     /locus_tag="OO135_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
BASE COUNT         1505 a         1471 c         1423 g         1357 t
ORIGIN      
        1 actggaaccc ctctttatat ttgcactttt ccgctgaacg atcgcgtccg gcggtggagc
       61 tgcttgccag agtgccgctg gaaaatgtcg aatatgtcgc cgatcttggc tgtggcccag
      121 gtaacagcac cgcccttcta caacaacgtt ggcctgcggc caggataaca ggcatcgact
      181 cgtctccggc gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag
      241 cagacatccg caactggcaa ccggtacagg cactcgatct gatttttgct aatgcctcac
      301 tgcaatggct gcctgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
      361 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
      421 gcgaagttgc ctgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
      481 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccacct
      541 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
      601 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttaag cgctaccatc
      661 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
      721 gtctttttat tgttgcccgg cgtatggagt aaatatcacg tcagctggta atgacgatcg
      781 ggaagaattt ttgctggaat ttcggcttca tcgttcatct gtaagaggtc aatttcaata
      841 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
      901 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
      961 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaaca
     1021 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tataggcaat gcgctcgcat
     1081 cctgccagga ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatttct
     1141 gaaagctgcc cattgcggcg ctgaaccgcc aaccactccc ccattattaa caagatacgg
     1201 ttagctggag agttcgaagc cagtacaggc tgaagatctt cagtcttgag ataatgagcc
     1261 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     1321 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcacgt
     1381 agtaacgacc gtgaggcaat catcaactgt ccccaaagtt ttcgcgcttc aacgtaacgg
     1441 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     1501 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaaaataaca
     1561 gcgatagaaa agagaaaatt gagtagtaag cgcgaggata tcttggataa tactgagccg
     1621 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     1681 aggcgtggaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     1741 caaattacct acaagcttac aacattaaca ggcgctgcat gtggcacctg ttaatgataa
     1801 aggcatatag gatgttgtaa ctaatatagt gaaataagaa acccggtcga aaccgggttc
     1861 agagtattag cacaacgggc gtacagcttc gcgcatccct ttttcaagaa tcgcatccag
     1921 gtcattagca acctgctcga ccagaccagg cacttgcgtc aggtcctgct cccagtggtc
     1981 tttttctgcc aaaacaatcg ctaccagttc cggggtgccg ataacacggt cacgatgctg
     2041 gctccagagt tgctgataac gttccagcca gtgtgcatcg tcctgtaccg gatatgtttc
     2101 tccgttacgc tcaccgcgat agaaggcaat caatgccgtt aatgcgaaag taaggcgcgc
     2161 cggaagtgtg ccgtttgcct tctgccctgc cagcagctgc ggcaggatgc gggtgcggaa
     2221 cttggtcata ccgttgagcg cgatagacag cagctgatgc ttaatgtacg ggttacggaa
     2281 acgcccggtg actgcactgg cgaaagattc cagttcatca cgaggcaaat ccagtacctg
     2341 aataatttct tcgtcgatgg ctttttcaac gaatgcgcaa atttcagcat cgttcatcgc
     2401 ttcacctacg gtatccagcc ctgcctggaa cgccaccggc accagcgcgg tgtgcgcacc
     2461 gttgaggatt gccactttgc gttctttata cggtttaata tcgtcaacaa tcagcacatt
     2521 gagcggatat ttgtccaggc gcagctcagt cgctaaggat ttcggcccct gaatcacaaa
     2581 caggtaaaag tgttcagcgg tgtcgagaaa accatcgtga taacccagtt cttcttccag
     2641 tttagccact tcaccacccc ggtagctgtc ggtgttgcgc gtcagcttag gcagagtgac
     2701 ggacgtcaca ttacccactt cgttttcacc atccacaaac agcgtaaaaa agcgaagatg
     2761 ttttggcaca gccatcaggc acctcccagc accgcaaatg cgggttcaaa gtattcatca
     2821 gtaaacgtct ggtaaagctc catgtcttcc agtggcggaa cgggcgtata tttgtagcga
     2881 atacgcacac gtccctgacg taaatccgtg gtgctgttat ccaccacgtc ataccagcac
     2941 tccgcgccaa tcagtttccc ggcagtcacc agtgaatcca gttttgccct gatggcactg
     3001 ataacatcct tcacattcgc aggcgtcagt ggactgtcga tggtttcaaa ctgcgcttcc
     3061 gcaattgaat cagccagcac ctgtgcggtt cgggtataca cctcaaagat gtaggcgttc
     3121 gtttccggtg tgcggttacc ccagaagcgg accccgttgc gacgaataat ggtcgtgatt
     3181 tctttgttgt tgaggctgtt ggcatcgctg tcttcggcct gcaacgacca gaacacatgc
     3241 ctcgacatcc ccagcacatt tttaaccgga acgttggaca gcgatttgtt ccatccctgt
     3301 tcatggtcaa tgtacgcacg aaggccgcac gcataggcag gcgcggggaa cgtttcgttt
     3361 ttgccatttt tcgggttgta ggcgatgaag tccggccata agagcatcac ctcacgttcg
     3421 ttgaatttct ggcggtaggt aattgcctcg gccatcgtgt tacagccatg acatgaggca
     3481 tacacaaacg cgcgcagttt atctgcaatc acgcacaggg attttgttac cgcctccgtg
     3541 tccagctccg gcgcggccag aatacgcgga cggtatccga tgctttcatc ctgctctgca
     3601 acaagcagcg catacatccc catatagctg ccgtcatcct cagaaccacc gataaccagt
     3661 tgatcctgcg tttttccgtc ttcttctttg tgttcagcca cgcgaacgac gatcaccttt
     3721 gtgctcacct ggtctgcgat ggccttaagc gcacgataaa gcgtccccgt tgtcccgcat
     3781 tttcccagca cgtcattgac gcgggtcagc agtgtgggct tgttcagcgg gaacagcttc
     3841 gcgtccgcat catccgccgt tgccacgata ccgataacgc tggaatcaac atcgttaatc
     3901 gctgttacca ggtcggtatt ctccgtaaca cgggcaccat gaaaacgagt ttcactcata
     3961 gcttcagccc cttgtatccg ttaaatgatt cagcaacaat catcacccac cacgcgcgta
     4021 atctcacccc tgcgccgttc tcccgccacg gcgacaacaa aaagcagtac ccccctccgc
     4081 acgcacatgc gaccatgccg cacagggagg gagcagatga ccgacaccac catgcaattg
     4141 ctcagtcagg gcacggcccc cgtgaaaatg ccggattttg atattctctc ggagggtaaa
     4201 acgctgtcag gcgtggcgga gcgcctgatg agcctgtaac tgaccgacaa ccggggattt
     4261 gaggcggacc agctcaccat cacgctggat gatgcggatg gtcagttgca gctaccgcca
     4321 cggggcgcgc gtctgacggt tctcattggc tggaaagggg aaccgctgac agaaaaaggc
     4381 acttacattg ttgatgaaat cgctcacgaa ggaccgccgg acaggctgac tgtttcagcc
     4441 agaagcgcag attttcggga tgaatttaac gttaaacgtg aggtgtcctg gcatgatgtg
     4501 accgttgagc gtgtggtatc cgccatcgct catcggtacg gtctgaaacc gcaaatcagc
     4561 gaaatgctga tggatatcga aatcgaccac gccgaccaga ccgaagaaag cgacatgtcc
     4621 tttcttacgc gcatggcgga aatgctggga gcaatcgcca cggtaaaaag cggcaatctg
     4681 ttattcatca tgccaggtgg tggcgtgaac gcacaggggc agccgttgcc ctcgttcgcc
     4741 attacacgca gcagcggcga tcgccatcag ttccgcattg ctgaccgcga ggcgtatacg
     4801 ggggtacgcg cctactggct tgatcttaat tacgggaaaa agaaaaaagt cagcgtgaaa
     4861 cgccgcaaac cgccaaaacc caaaaaggag aaaagcagca gtcgggaagg cgactatatg
     4921 gaaggtgcgg aaggcaacgt gtttgtgtta cgcaagactt atcagaacga gcaggcagca
     4981 agacgcgcag cggcggcaaa gtggcagcag ctacaacgcg gagccgcatc attctccatc
     5041 acgctggcgc gtggtcgtgc agaactctat cccgaaatgc atggcacagt gacaggcttt
     5101 aaaagcgaga ttgattatca ggactggatt attgcaaaag ccgagcacac cattgataac
     5161 agcggcttta ccacgcagct tgaacttgaa gcaaaaatcc cggaatggat agcagaaaca
     5221 gaataaatct cttaaaaata ttatttttga gatgtgcttc tacactgaca gtaagaacaa
     5281 aagataatgt atcagcccac tagatgtcag aggttggggc atcaaccaga tgctaacaat
     5341 aatacggatg tttttcccac atgacagagg aagtcaagta taatcttcac agtccatccc
     5401 gtccaaggga atatgactca atagtaaaag ttccagttta gttagtagac ccacacttta
     5461 tagaagagta attcatgatt ggacaatttt taagtgcaac agagatttta gcaaagaact
     5521 atgttcgtaa taaaatggtt aaaaatccat tttattcgaa tttaaaatgg aattttattg
     5581 aaaaaaacat cataagatta acatcctcac cggttaaatc agtactttgc atatcagctt
     5641 tttcatttgt actactttat gttggatatt taaacgaatt atttataaaa aataatcttt
     5701 tacattattt cccgtttagg cattcattaa ccgaatggca aacaaccatt ttaagt
//
LOCUS       DAJTMK010000220         5694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTMK010000220 DAJTMK010000000
VERSION     DAJTMK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.224"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(246..4103)
                     /gene="sigA"
                     /locus_tag="OO135_003767"
     CDS             complement(246..4103)
                     /gene="sigA"
                     /locus_tag="OO135_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5399432.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSEKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFQASTQAIGFSNVSPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4464..5467)
                     /locus_tag="OO135_003768"
                     /pseudo
     CDS             complement(4464..5467)
                     /locus_tag="OO135_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5464..5694
                     /locus_tag="OO135_003769"
                     /pseudo
     CDS             5464..5694
                     /locus_tag="OO135_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1420 a         1278 c         1267 g         1729 t
ORIGIN      
        1 tttcgttata ctgtccgtat cggaaccatg agaactcagt tgcattccag ccatgctcca
       61 gaatatgtgc atttttttgc ctgatagttt ctttttttat tcgctcatgt ttaaggcata
      121 acaggcagat aaataatgtc agaaaaagaa agctaataat gagagcaaga gcgatatagt
      181 ctgtcacagg gtaatacata ttctctccgg tagaagaagg gccgcaaacg cggcccgggc
      241 tgttatcaga aagagtaacg gaagttggcg ttgatcgcgt tatccacgtt gtatttacca
      301 aatgccgatt tctcaaactc aagaccgaag cgaagattat cgcgaatttc ggcgttgaga
      361 ccaacattca tgagaatacg accgtctttt tcacctttga tacgtttctc accggacgca
      421 tcacgcagta cggtttcacc gttggcaaac aggtcaaact ggtagccaag gccggcgcgg
      481 gctgtgactt tccagtcctt accggagaag gatttaccca catcaacacc ggtacgccca
      541 atcagcggat taaagtcctt atccttcatg gtcacgctca ttccgcgatc ctgccagtcg
      601 aaccgtttac ctgatacggc cccataaacc agttctgcct gcggctcaat ccaggagtct
      661 tctgttacat gatagcggta gcctgcttca gcacccacat acaaggaatg agaactgtag
      721 tcttttgttc cgagcccagc aaaggtcgcc gagtactcat tatcatggtg aacatactta
      781 ctaatcaggt cgatataggc accagagtca aatatagcgg aagcatacag acctgccccg
      841 acggacttcg tcttgccact gaatgcatta ctgctggcat gactgtcggt atacgtcata
      901 gtcagaccag tgaaaaggtc aagtccatcc agctcatgtt ttttatccac accaatctgc
      961 acatgggtgt agttgtcact gtatccacca gaagctgaac ctgctccgct catgatgcgg
     1021 gcccatgcac cggcctcgcc attggtgtca cgcagatccc ccattcgttt attcaggttg
     1081 ttgacttctg tcaggaaggc tttgtaatta ccggacatat acgattttgc tgcactggaa
     1141 gatgctgtat tttcagccac cttatatccg gtcagggtcc acgtggtatg ttcctgatcc
     1201 tctttcgtgc tgatcacagg agatacgttg ctgaaaccta tggcctgagt actggcttga
     1261 aatatctctt tattgcttcc ttttggggct gttatcaggt caacgtttaa tcctgagtta
     1321 ttatttccca ctttctgtat atcgaccgac agactgttat tgcttccggt taactgattt
     1381 ttaacaatga gtttatccga ttgatttaca gactggctca gatcagcccg cataatcaca
     1441 gcagtattag tggcatccag tgtgtttact gtcacagtat ggaaacccga attctctatg
     1501 ttactaaaca acaaaagact gtcgcgggtt tttaatgatt gcagggctga ctgtccgttt
     1561 actctccata aggtgttata catgctggct gaggcagcag gagcatcaat acctccggta
     1621 taagaagtat caaagccacc cagcaaacct gtagccagcg gtgtgtgtgt ggttttgtcc
     1681 aggttatctt ctttatactg gtcaacacca aacagaatag atgctttttt ttctgatttt
     1741 atattaccat tgacccaggc ataattttga gcctttaacg tggcaccatc ttctgtcata
     1801 ttatatccat ctgacatata gattgccggg ctgtatttat tattttctgt aggggtgcct
     1861 gttaatgtca gggttccggc cagttcaata gctttatcta catacatttt tccctgggcg
     1921 gtaacagtag ctcctttgtt gataatcagg tctgtattaa ttaaggataa tgcaccagtc
     1981 atagtaacag ttgatttatc accaacagtt attttgctgt tttgagcact cagactgccg
     2041 ttgaaactac tttcgcctgc tgaatccccg gagcgaagct gttgtctgaa tccaaaacct
     2101 tcgccagtaa tgtttcttcc tgagaacatg tcaatgtatg cagttttatt tcccagaaca
     2161 atttgagagt ttttagcctc tatgtgtcct tttacattag catcccggcc tatactcagg
     2221 gttgcgtttt ctaaatttaa tttccggaaa ttaaatttac gactttccca gtcgggctgg
     2281 gaaaaagagg ctatctgatt gttggactta tattccgtat tgtaacgttt atttacagtg
     2341 ctttcctggt cctttatggc agcagaatag tctttttgac ataaaaaagg aattggacag
     2401 ttgttattgc cttctttaaa tatggcatgt tctgtcgcat gtccttgtaa tgttaactgt
     2461 gcattccgga cagagatatc gtttttgata tcgactgagc catcaaagat cagattggca
     2521 ttattgttat gttgctgggt agtattgata atatgattta tgcttatatt acctgataca
     2581 ttcccatggt acatatatgc atcagtatta gtaagtgata atgttgattg cttcacgttt
     2641 gagttcgtta cggttgttcc cgcatctgtt gctgcgattt tctgaaatga ctggtcatag
     2701 ccgtttagat ccagtgttcc gccatttctg gtaaaataga tttcgccatt accgctttcg
     2761 cttaaagcgt ctttggcatt tatttttacc gtacctttac ctccggccat gtaaatttta
     2821 ttgaaggctt tttcagcact aaggatgaca gtcccattac ctattttaag gttatttccc
     2881 tgtgctattt ttacatccag agtaccacta cctattttat gcagattatc tccggcaacg
     2941 cccttaacat tccattcaac ggtggtatta ttatcaatat ctatcccggc acctttataa
     3001 gacttatctt ttccgtacac tttatatgtc tggttcttat cgaatataag cccgccaatg
     3061 ccaagatcgg tatcctgctt gagcgttaat tcccctcctc ctgagacaga caggtcctta
     3121 tcttttataa tcgatgataa ctcttgtttt ttaccatcca gctgcatagt gtttgtgaaa
     3181 ttctttttta aattgtttac cagaatatta ttaaataacg agtagtttgt ccaggctata
     3241 ttggtactac tgataaaacc aactgtactg gtaacaccat ggaaaaccca tttttgttgt
     3301 tctttatcat atagataaga accagaacca ctatcgccaa aaattggtga gtttttaaaa
     3361 ggggttctgt tggtataaat gtaactcttg tttttccagt ccagattaaa aagacttgcg
     3421 cttaacaagt cgggattata gttttgtcca aacagatatt gtttgccgtt taatatcgtt
     3481 gaggttgttc cggcacctgc tctgaagcca attatttgtt ccttaccgtt gtagtttacg
     3541 ccatatcttt tcatcgcatc ttcgggagaa agtgagaagt cggcactctc cgtaacccct
     3601 gttgtttcca cgacgaattt attcagacga tgaaccgaaa agtcgccaga tgatactctg
     3661 ttacttgctt tatatgctgt ctgatcccat gactgtgttg ttattgcatg atgctgtgta
     3721 ccgttatgag tcgcagtaac actgtacgca cctccaattg atgttgttgc acctttgttg
     3781 gaaacagcag aaaaatcagg tacaggtagt tctggaaaat gaaatatctt tccatctttc
     3841 atcattaatt gaacatttgt tgcacccggt ctgaactctc ctctgttttg agcaaggtca
     3901 agataatcgc gggtccatat attatttata tccagcattg ctgcaaatgc tgtttcgcca
     3961 taacttccat atattgagga taaaataata ccgaggataa ctttctttct tgatgtaccg
     4021 acactaactt tccgggtcag ttcagaaaca gcaactaatc cacctgtaat atgactatat
     4081 ttcagtgaat aaattttatt catacgtgaa ctccgatatt tctgagttgt ggttatggga
     4141 tagcatttaa ttacaacggg gaactatcag tcatggtacg taactgataa gggtataatg
     4201 ataatcactg tcagttatat ttgtatgaca gatgtaacaa aatgaaaaaa aagataagca
     4261 caagtgattg attggtcaga tattgatcaa ttggtgtttg tgttttgaac tttgcgatca
     4321 aaatttgatc tttgttgttt ccggtcaatc ctttacaggt tttctgtatt atccggggga
     4381 gttaagtctg tcgatgtccg acagactttt gatgatggaa acaacatgca gcgataatgt
     4441 gcatgctgtt ttgcaggtgg aactcagaag gtgaactgga actgtgcgac cgcaccgtaa
     4501 gtgttgtccg tgccgatcag gcctgcaaca tcaaggtgaa ccacatcaaa cggtgagagc
     4561 cccaccccgg ccgtgaatgc actgccgtcg ttaccggcca gattctgacg gtaaccggcg
     4621 cgcagctgtg cccatttcca ggcattaaat tctgcgccaa tggcggcaaa ctgacggttg
     4681 ctgtcggagg tgaaaccgct ggccgggtca gatccacatc aaatgcggtg gtgaacatcg
     4741 cgttgtgcca ggagacaccg gccgtcacct gcgggcggat cctgaaggtt tccgtgatac
     4801 cgttcacttc ttttgtctca atactacggg ggatcaggtt ctgtgcgacc agtcccagcg
     4861 tccagttgtc atctaggtcc gtactggcac cgatgtcggc gttgatgcca tttttcgtgt
     4921 tgtggtagcg gtcgcctttg aaggcgctgc tgtcataatt tctgaccagc acgttgtagt
     4981 tgaacaggtc gacgcgctgg aatttggggg ggaagcccag agaccatttc tgcccggccg
     5041 tttccagttc cgtagccaga gcaataccca catcagtgat cagtgccgcg cgggcaaagg
     5101 cctgtgatgt caggcggctt ttgtccacct cctgcccggt gctgtttgcc acttcttcca
     5161 ggtaagtgag gtcagcatca gagactttac cgtcgacact gacggtgccg tgtgatttaa
     5221 ccatcagtgc ggcagagagg cgatcaccgg gaagggccgc cacagcagaa acgcccagct
     5281 gtgcggcggc atgtgtgtta cggaagtccc ggaggtgttc tttcaggcgt gcggcagcct
     5341 caggaacacc gtggttactt tcgatggcgc ggtcgaaggc cttccactca tcactgatcc
     5401 ggtcggcgtt gtcggtgata ttatccggat tggagagctg cgcgccgacg gacggaagca
     5461 ggatgctggg ctcatgcgcg acgcaaaatc cacgatgtat atatcagtac taaaagcgcg
     5521 acggcggaag aagctctgaa actaatcggt gaactgtacg ccatagagca cgatatacgc
     5581 gggttaccgg tgtctgaacg cctggcggtc aggcaaatgc agagtagacc gctcctgact
     5641 tccctgtata agctgatgca ggagaaagaa cacacgttat cgaagaaata tcgt
//
LOCUS       DAJTMK010000221         5596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTMK010000221 DAJTMK010000000
VERSION     DAJTMK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.226"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(122..1460)
                     /gene="adiC"
                     /locus_tag="OO135_003770"
                     /pseudo
     CDS             complement(122..1460)
                     /gene="adiC"
                     /locus_tag="OO135_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
     gene            complement(1597..2358)
                     /gene="adiY"
                     /locus_tag="OO135_003771"
     CDS             complement(1597..2358)
                     /gene="adiY"
                     /locus_tag="OO135_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5399433.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2683..4950)
                     /gene="adiA"
                     /locus_tag="OO135_003772"
     CDS             complement(2683..4950)
                     /gene="adiA"
                     /locus_tag="OO135_003772"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5399434.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(5149..>5596)
                     /gene="melR"
                     /locus_tag="OO135_003773"
     CDS             complement(5149..>5596)
                     /gene="melR"
                     /locus_tag="OO135_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5399435.1"
                     /translation="PEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHTQFYVSQMLG
                     FIAENYDQALTINDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTD
                     KSILDIALTAGFRSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         1439 a         1321 c         1427 g         1409 t
ORIGIN      
        1 gcaggaagga catctgatgg aaatggaagc cacgcaagca ccttaaaatc accatcatac
       61 actaaatcag taagttggca gcattaccgg ttggctttta tgtttgctgg attaagggga
      121 attaatcttt gcttattggt gcatctaagg gatacgggtt tttatgtagc cggttgtaat
      181 tcagcgcata catggcggtg atgaccatca gggtgacaaa tgaccacata acctctttcg
      241 ctccggaccc caccacggcc cagatgcagt agaggaaggc aatggtagta actgccagat
      301 atgccgggcg tgctttacca aagtgaccgt gtccgagcag cagtaacgcc gcacaggtgt
      361 aaagatatgg caccagtgta aagatgaccg agacggaaga aaccagaccg aactctttgg
      421 tcgcgtttgg tgaaatgctg ctgagctgga agatggtcat caaaataccg acgataatca
      481 gccccgccac tggcgtaccc gctttattta cacgggcaaa aatcggtggg aacagtccgt
      541 catcggcagc ggctttcgcc gtttgacccg ccagcaacgt ccagccgccc aagtgaacct
      601 aagcaacccg cagctgcgca gaaggaaaca atggccccgg cggtgtcacc caacgccatc
      661 cgtgcggcat caccgaatgg cgaagcagaa acgcgcagtg cggcattagg gatcatcccc
      721 ataatcgcgg tggtagaaag tacatagcaa acggcggcaa tcaatacccc accaatggtg
      781 gcgataggga cattgcgttt cgggtttttc accacacctg cggcaacgga ggcactttcc
      841 acaccgatga acgaccacag cgtaacgtta agggtacttt gaattgcacc gaaggtgccc
      901 aggccgctga cgttccatgc cgccatatag gtttcaccac ggaaccagaa ccagccaaat
      961 acggcaatcc cgacgatggg gatcagcgcc agcacggtgg caactgcctg cacacgggtg
     1021 atcattttcg gaccgacaat gttcagcagg acgaagatcc acagcaccac gacgcaggtg
     1081 atggttaata ccaatggatc tttcagaatc gggaagaagt aacttaaata tcctacgcca
     1141 atgaccacca tggcgatatt gccgatccag caggccagcc agtagaggac gttggtttga
     1201 taaccgagaa acgggccaaa gcagcggcgg gcgtaagcgt aagaaccacc aggacttggg
     1261 tcgaggaacg acattttggc gtataccatc gagagcccca gtgcaccgat aatcgtcacc
     1321 aaccatccat aaatggcaat cccgccagtg gaggccaggt ttgcaggtaa cagaaaaaca
     1381 cctgacccca taatattccc cgacaccatc agggtgacgg ggattaagcc cactttgtga
     1441 gcatcagcat ccgaagacat aattaaactc ctgcgaaggc gagcttcgtg acaataaatt
     1501 acgtcatatc atacgcctgc attcgatagt tgaattatta tcgaccgggt tatcgctgat
     1561 gccgttatta atatagctct tcgttaaggt taaaaatcag gcgacagtgc gttctctgtg
     1621 ctgactaaca taatgcagcg gcgtcatacc gtagaagtct ttaaagacag aaataaagta
     1681 cgacgtactg ttgtagccgc aggactgtga tacctgagag atatttttac cgtccatcat
     1741 taattcattt acggcataac gcatgcggca ggtggtgatt atttggctat aactggtgtt
     1801 ttcgcttttc agctttttct ttaacagact tgggctaaga cataaacaac tggctaccat
     1861 actaagattc cagtctttgt gaatatcgct ttcaataatt tgataaacgc tgtcacttac
     1921 acaattacgt aacatataca tcattaatga aaggaatttt ttactgtcga gaaaacggga
     1981 tagcaccgtg aataataatg cgcgtgttcg ttccttttct gactcagttt ctgcgggcat
     2041 catgctgtgt tgcgcggcaa cgcgaaagac gtctggcgtc aggcatggca gggtgagtat
     2101 gggcgtagcg ctacgttgcc atactggtat ttgcgagaga tctttattca gaaaccggag
     2161 gtaatctttg atgatgtcgt gactaatatg agcaaccaaa gtgttgttaa gagaggagac
     2221 gtcgataata ttattttcac aatttaataa cgccatatgg ttagctttaa ggctaatagg
     2281 ttctttccca ttcaccctta tccagacatc tttttcagtc aataaaacaa tacaaggttg
     2341 gtcgctgcaa atcctcatac atgtactcct gagtgcgaat aaaagaccag ttctcgctaa
     2401 agcaaagcga taccgggaaa tggttatata attgtatgac gaatataaac atgcataaaa
     2461 ataacgagtt ttactaatat tacaaatttt tatagtaatt tctcatgtac atattttttt
     2521 acagggtaaa taacaggcaa aaaaaagcgc ggggtggggc cgcgcaaaaa gtatgacatt
     2581 tttgtttgca gcaattttta tgaaaacgtt gccatactca aaaggcctaa ggccaggcgg
     2641 cgtaatgtta tttaaacaat tacgccttca gcggaatagt ggttacgctt tcacacacat
     2701 aacgtggtaa ataccgtcaa taatttcagt cccttcagtt tcgtgttcaa atccagggaa
     2761 atggtggtcc caggattgca gcgagcgtaa ataacttact tgcggactgt ttttatcgcc
     2821 gaagttttca ccagacagca gcatcgggat tcctggcgga tatgggataa ctgagtttgc
     2881 cgcgatgcgt cctggcagat tttcaatgga taccagttcg acattgttgt cgacaatcgc
     2941 gttgtacgct tcacgcgggg tgatttccgc caccggcagg ccggaatagg cttcgttcaa
     3001 ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg gtgtcaccca gatcgtgaat
     3061 ccccatgttc gcgtaagtgt caggatattg ttcaacaagt tccggcatca cctgcgccag
     3121 cggtgtgttg gcgtcatagt ggcgtttgaa ggagcaaagg gtgttaacca gagtccccca
     3181 tttcccacgg gttacgccca tagagaacag gaacataatt tggaagtcag tggtgcgggt
     3241 aggcacaatg ccgtggcgac caagccaggc agtgaccagc gccgccggaa caccggtttc
     3301 ttccagttca ccatcttcac ccattcccgg agcaaggatg ctgactttaa tcgggtcgag
     3361 catactccag ttatccggaa tatctttgaa gccgtgccag ctttcgcccg gatgcattac
     3421 ccagtagtcc tgaacggtgg tcagcagttt ggttggtgcg tcagcaaagt cataggtttt
     3481 gccggtttgt gggtcggtga cgacttcttt gttccacggt ttgaagaacc agctaccgtc
     3541 agcggtgaac tctttataca gccgcgccat cgcctgacgg aaatcaaccg cttcgtcgat
     3601 cacttcctgc gtcagtgaca ggccgctgtt gccgtccatc atcgacaccg ccacgtcgtt
     3661 ggatgcgcag atggcataca gcggggaggt ggtggcatgc atcatgtagg cctggttgaa
     3721 gcgggagaag ttaatcgccc cacgaccttc acgtacatga atataagaag cctgtgaaag
     3781 cgcattcagc agtttgtggg tggagtgggt ggcgaaaacg gtaggaccgt tgtgatcgcc
     3841 aggttcgccg cgcatggcat agtgatcggc atagatcggg ttgaaacgtg catagccgta
     3901 ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc agcagatcct gcgcttcttt
     3961 agcgttataa cacacgccgt cataggtgca gttggtcacc acgcagtaag acggtttttg
     4021 cccggctttg tctttagtca gcgggctttc actgattttc ttctgcaagg tttcaggttg
     4081 catttcctgc ggatagattg gcccgataat gccgtagcgg ttgcggcttg gcaccatgta
     4141 gaccggtttc gcgcctgtca gcatcaagcc ttgttcgatg gatttatggc agttacggtc
     4201 aacgaccacg acatcgttat cggtcatgca agcctgcatg atggtgcggt tagagccgga
     4261 agtaccgacg actaccgacc aggagcgatc ggcaccaaat acgcgtgcgg catatttttc
     4321 gctttcgcca aatgcgccag tatggtcaag caaagaaccg agggaagttc gttcgatgcc
     4381 catatcggtg cggaacagat tttctccata gtagtcatgg tagaaacgtc cggcgggcgt
     4441 tttggtaaaa ccaacgccgc cctggtggcc tggcgctgcc caggaatatt catggatgtc
     4501 gctatatttc atcagcgcgc tgaacagtgg cggcaacagc tgctggcggt agcgggtcat
     4561 cgcggcaacg gcgcgtccgg cgataaagtc ggtggtatct tccagaatcc aggcgaattc
     4621 atcgacaagc tccagcaggt cgcgatccat tgcggcgagg gctttttccc gatcgcccaa
     4681 caggaagacc ggcacgtttt gttggcgctc atgaagctta ccgatcaatt gtctgacgtt
     4741 ttgatgttcg tccggatgtt ccatttgata gctgaacatc aggcagtcaa tggcttcgtt
     4801 tgaagagaga atggcaaaac catcatcaaa ggaggtggat ttaatcacgg taacattttg
     4861 ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg acccaggtgt cttgatggag
     4921 aaactcgctt tcaacaatta atactttcat cattgcttac ccggttatga aggaatcttc
     4981 gttgcgggca agtatcttcc ggccttttcg ggctgtaaag cgcgtgaaaa acaaacattt
     5041 ttgaaatatt tatttttata aacaatgcgt tatgtgatta ttttaaatta ttttcactgc
     5101 ttgttatcga cggctaaact atttttttgg ctgatactga tatcgtcttt agccgggaaa
     5161 cgtctggcgg cgctgttggc taagtttgcg gtattgttgc ggcgacatgc cgacatattt
     5221 gccgaacgtg ctgtaaaaac gactacttga acgaaagcct gccgtcaggg caatatcgag
     5281 aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca tcgcggtaat
     5341 gtactgtttc atcgtcaatt gcatgacccg ctggaatatc cccattgcat agttggcgtt
     5401 aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt tttcggcaat
     5461 aaagcccagc atctggctaa cataaaattg cgtatggcgc gagacgctgt ttttgtgtgt
     5521 gcgcgaggtt ttattgacca gaatcggttc ccagccagag aggctaaatc gcttgagcat
     5581 caggccaatt tcaggt
//
LOCUS       DAJTMK010000222         5581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTMK010000222 DAJTMK010000000
VERSION     DAJTMK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.227"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            26..241
                     /locus_tag="OO135_003774"
     CDS             26..241
                     /locus_tag="OO135_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5399436.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            234..620
                     /locus_tag="OO135_003775"
     CDS             234..620
                     /locus_tag="OO135_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5399437.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            628..1314
                     /locus_tag="OO135_003776"
     CDS             628..1314
                     /locus_tag="OO135_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5399438.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1292..1915)
                     /gene="tetR(B)"
                     /locus_tag="OO135_003777"
     CDS             complement(1292..1915)
                     /gene="tetR(B)"
                     /locus_tag="OO135_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5399439.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1997..3202
                     /gene="tet(B)"
                     /locus_tag="OO135_003778"
     CDS             1997..3202
                     /gene="tet(B)"
                     /locus_tag="OO135_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5399440.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3315..3908)
                     /gene="tetC"
                     /locus_tag="OO135_003779"
     CDS             complement(3315..3908)
                     /gene="tetC"
                     /locus_tag="OO135_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5399441.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4041..4412
                     /locus_tag="OO135_003780"
     CDS             4041..4412
                     /locus_tag="OO135_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399442.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4422..>5581)
                     /locus_tag="OO135_003781"
     CDS             complement(4422..>5581)
                     /locus_tag="OO135_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5399443.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1542 a         1028 c         1215 g         1796 t
ORIGIN      
        1 actattaagt catgtagctg gttttatttc aattgaacgt tttcaaagtc tagctacaga
       61 aggaaaaatg ttatcgctat cttggtggga aaacgaatac gcagttctgc aatggaaaaa
      121 tcatgtttta catgcgaaag ctcaacaaga agggcgagag tcaatatttg atttttacaa
      181 aattagtatt gctcatatta ctcgcgaata ttcatttaaa aaggacaagg ataatgtttg
      241 atgttcacgt tgttttagat aatcaaatag gacaattagc attactagga aaaacattag
      301 gtaataaagg tattggattg gaagggggag ggatatttac ggttggtgat gaatgccatg
      361 ctcattttct tgttgaacaa ggaaaggaag ctaaaatagc gctagagcaa gctggactgt
      421 tagtacttgc gatccggaca ccattaattc gtaagttaaa acaggaaaaa ccgggggaac
      481 ttggcgaaat agcacgagta ttggcggaga ataacattaa tattttagtg caatacagtg
      541 accatgctaa ccaactgata ttaataacgg acaatgatag tatggctgca tctgttacgc
      601 tcccttgggc aataaagtga acttgcgatg gctaatttaa tacgaaaaga ggttaccttt
      661 gagtcctcaa tagccgcgat aggggcggct atgtctgaca tttcacgagt taaaatactc
      721 agtgctttga tggatgggcg agcttggacg gccactgagc taagttctgt ggcgaatata
      781 tcagcttcaa cggcgagcag tcatttatct aaattattag attgccagct aatcacagta
      841 gtagctcaag gcaagcatcg ttattttcgg ctagcaggaa aagatattgc tgaattgatg
      901 gaaagtatga tggggatctc cttaaaccat ggcgtacatg ccaaagtttc cacgccagtg
      961 catttacgaa aagcacgtac ttgctatgat catttagctg gcgaagttgc cgttaagatc
     1021 tatgattccc tttgtcaaca gcaatggatc actgaaaatg gttcaatgat cacattaagt
     1081 ggtattcaat attttcatga aatgggaatt gacgttcctt ccaaacattc acgtaaaatc
     1141 tgttgtgcgt gtttagattg gagtgaacgc cgtttccatt taggtgggta cgttggagcc
     1201 gcattatttt cgctttatga atctaaaggg tggttaactc gacatcttgg ttaccgtgaa
     1261 gttaccatca cggaaaaagg ttatgctgct tttaagaccc actttcacat ttaagttgtt
     1321 tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt
     1381 gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt
     1441 ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat
     1501 gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa
     1561 aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta
     1621 cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta
     1681 aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca
     1741 tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag
     1801 gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct
     1861 cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta
     1921 attcctaatt tttgttgaca ctctatcatt gatagagtta ttttaccact ccctatcagt
     1981 gatagagaaa agtgaaatga atagttcgac aaagatcgca ttggtaatta cgttactcga
     2041 tgccatgggg attggcctta tcatgccagt cttgccaacg ttattacgtg aatttattgc
     2101 ttcggaagat atcgctaacc actttggcgt attgcttgca ctttatgcgt taatgcaggt
     2161 tatctttgct ccttggcttg gaaaaatgtc tgaccgattt ggtcggcgcc cagtgctgtt
     2221 gttgtcatta ataggcgcat cgctggatta cttattgctg gctttttcaa gtgcgctttg
     2281 gatgctgtat ttaggccgtt tgctttcagg gatcacagga gctactgggg ctgtcgcggc
     2341 atcggtcatt gccgatacca cctcagcttc tcaacgcgtg aagtggttcg gttggttagg
     2401 ggcaagtttt gggcttggtt taatagcggg gcctattatt ggtggttttg caggagagat
     2461 ttcaccgcat agtccctttt ttatcgctgc gttgctaaat attgtcgctt tccttgtggt
     2521 tatgttttgg ttccgtgaaa ccaaaaatac acgtgataat acagataccg aagtaggggt
     2581 tgagacgcaa tcgaattcgg tatacatcac tttatttaaa acgatgccca ttttgttgat
     2641 tatttatttt tcagcgcaat tgataggcca aattcccgca acggtgtggg tgctatttac
     2701 cgaaaatcgt tttggatgga atagcatgat ggttggcttt tcattagcgg gtcttggtct
     2761 tttacactca gtattccaag cctttgtggc aggaagaata gccactaaat ggggcgaaaa
     2821 aacggcagta ctgctcggat ttattgcaga tagtagtgca tttgcctttt tagcgtttat
     2881 atctgaaggt tggttagttt tccctgtttt aattttattg gctggtggtg ggatcgcttt
     2941 acctgcatta cagggagtga tgtctatcca aacaaagagt catcagcaag gtgctttaca
     3001 gggattattg gtgagcctta ccaatgcaac cggtgttatt ggcccattac tgtttgctgt
     3061 tatttataat cattcactac caatttggga tggctggatt tggattattg gtttagcgtt
     3121 ttactgtatt attatcctgc tatcgatgac cttcatgtta acccctcaag ctcaggggag
     3181 taaacaggag acaagtgctt agttatttcg tcaccaaatg atgttattcc gcgaaatata
     3241 atgaccctct tgataaccca agagggcatt ttttacgata aagaagattt agcttcaaat
     3301 aaaacctatc tattttattt atctttcaag ctcaataaaa agccgcggta aatagcaata
     3361 aattggcctt ttttatcggc aagctctttt aggtttttcg catgtattgc gatatgcata
     3421 aaccagccat tgagtaagtt tttaagcaca tcatcatcat aagctttaag ttggttctct
     3481 tggatcaatt tgctgacaat ggcgtttacc ttaccagtaa tgtattcaag gctaattttt
     3541 tcaagttcat tccaaccaat gataggcatc acttcttgga tagggataag gtttttatta
     3601 ttatcaataa tataatcaag ataatgttca aatatacttt ctaaggcaga ccaaccattt
     3661 gttaaatcag tttttgttgt gatgtaggca tcaatcataa ttaattgctg cttataacag
     3721 gcactgagta attgtttttt atttttaaag tgatgataaa aggcaccttt ggtcaccaac
     3781 gcttttcccg agatctcatc tattgaaaca gcttgatagc ctttttcaac aaacaatatt
     3841 cgtgctgagt taaccagtga ttgataggta ctcttaaaat tttcttgttg atgattttta
     3901 ttttccatga tagatttaaa ataacatacc gtcagtatgt ttatggtatc atgatgatgt
     3961 ggtcgtgaca atcttaagaa catttaggtt attttatgta tattgaacag cattctcgct
     4021 atcaaaataa agctaataac atccaattaa gatatgatga taagcagttt catacaacgg
     4081 ttatcaaaga tgttctatta tggattgaac ataatttaga tcagtcttta ctgcttgatg
     4141 atgtggcgaa taaagcgggt tataccaagt ggtattttca gcggctgttc aaaaaagtaa
     4201 caggggtcac actggctagc tatattcgtg ctcgtcgttt gacgaaagcg gctgttgagt
     4261 tgaggttgac gaaaaaaact atccttgaga tcgcattaaa atatcaattt gattcccaac
     4321 aatcttttac acgtcgattt aagtacattt ttaaggttac accaagttat tatcggcgta
     4381 ataaattatg ggaattggag gcaatgcact gagagatccc ctcataattt ccccaaagcg
     4441 taaccatgtg tgaataaatt ttgagctagt agggttgcag ccacgagtaa gtcttccctt
     4501 gttattgtgt agccagaatg ccgcaaaact tccatgccta agcgaactgt tgagagtacg
     4561 tttcgatttc tgactgtgtt agcctggaag tgcttgtccc aaccttgttt ctgagcatga
     4621 acgcccgcaa gccaacatgt tagttgaagc atcagggcga ttagcagcat gatatcaaaa
     4681 cgctctgagc tgctcgttcg gctatggcgt aggcctagtc cgtaggcagg acttttcaag
     4741 tctcggaagg tttcttcaat ctgcattcgc ttcgaataga tattaacaag ttgtttgggt
     4801 gttcgaattt caacaggtaa gttagttgct agaacccatg gctcctttgc cgacgctgag
     4861 tagattttag gtgacgggtg gtgacaatga gtccgtgtcg agcgctgatt ttttcggcct
     4921 ttagagcgag atttatacaa tagaatttgg catgagattg gattgctttt agtcagcctc
     4981 ttatagccta aagtctttga gtgactagat gacatatcat gtaagttgct gataggtttc
     5041 cagttttccg ctcctaggtc tgcatattgt acttttcctc ttactcgact taaccagtac
     5101 caacccagct tctcaacgga tttataccat ggcactttaa agccagcatc actgacaatg
     5161 agcggtgtgg tgttactcgg tagaatgctc gcaaggtcgg ctagaaattg gtcatgagct
     5221 ttctttgaac attgctctga aagcgggaac gctttctcat aaagagtaac agaacgaccg
     5281 tgtagtgcga ctgaagctcg caataccata agtcgttttt gctcacgaat atcagaccag
     5341 tcaacaagta caatgggcat cgtattgccc gaacagataa agctagcatg ccaacggtat
     5401 acagcgagtc gctctttgtg gaggtgacga ttacctaaca atcggtcgat tcgtttgatg
     5461 ttatgttttg ttctcgcttt ggttggcagg ttacggccaa gttcggtaag agtgagagtt
     5521 ttacagtcaa gtaatgcgtg gcaagccaac gttaagctgt tgagtcgttt taagtgtaat
     5581 t
//
LOCUS       DAJTMK010000223         5482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTMK010000223 DAJTMK010000000
VERSION     DAJTMK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.228"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(23..595)
                     /locus_tag="OO135_003782"
     CDS             complement(23..595)
                     /locus_tag="OO135_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5399444.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            813..1673
                     /gene="tesB"
                     /locus_tag="OO135_003783"
     CDS             813..1673
                     /gene="tesB"
                     /locus_tag="OO135_003783"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5399445.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO135_003784"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO135_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5399446.1"
                     /translation="MKIAMIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO135_003785"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO135_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5399447.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO135_003786"
     CDS             complement(3556..5337)
                     /locus_tag="OO135_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5399448.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..5476)
                     /locus_tag="OO135_003787"
     CDS             complement(5330..5476)
                     /locus_tag="OO135_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5399449.1"
                     /translation="MQHGHIAQRGNHDELAQQSGWYRDMYRYQQLEAALDDAPEIREE
                     AVDA"
BASE COUNT         1331 a         1595 c         1363 g         1193 t
ORIGIN      
        1 gggcggagag ggttaacgtc gcttaatatt gcgtaggagc tggaactgcc gaagacggat
       61 tcacctgagt tggtgaagtc gaaggtacgg tagtcgttgc accaccgctg gcctgaaccg
      121 gcacggcggt ttgctgcacc ggcaccaatg tcaggtcggc tttagttccg cccttgttga
      181 tcaccggctg aacggtatcg gtaataaata ccagtttgtc attcacggta atcgccgcac
      241 tcaacagaat acgcgcgttc ggctgaacat ctgccgggtt aaatggcaga acaaagctga
      301 atggtgactg tttaccttcg gtacgcaccg ctttctgcgc cagcactttt gacggtgcat
      361 cggctaacga cgcgtcagaa agtgtcacgg tcagcacagc atcaggcggc agtgcgactt
      421 tctgccggat ccagacggta ccggagacat tcggttgctg gatagcaggt tgttgtgttg
      481 ctgaaataga cgtatttgca gccggggctg gcgtctgaat atccgcgctt ttatctgcgc
      541 aagccgccaa cgcaatcgca accgctaaac cactggccat gtgcacgagt ttcattcatt
      601 tctccttatt atcaatgcac cagcgggcta actttcctcg ccggaagagt ggttaacaaa
      661 atagtaacgt caataagtgt ggcacacatc acgcatttct gcctgtaatt agcccgtaat
      721 tcagattatt gcacccatcg gaccagtggc aaatttgcgt tatactcaac tcactttggc
      781 ttgctgcggc agctttgtta ctggagagtt atatgagtca ggcgctaaaa aatttactgg
      841 cattgttaaa tctggaaaaa attgaggcag gactctttcg cggccagagt gaagatttag
      901 gtttacgcca ggtgtttggc ggccaggtcg tgggtcaggc cttgtatgct gcaaaagaga
      961 ccgtcccgga agagcggctg gtacattcgt ttcacagcta ctttcttcgc ccaggcgata
     1021 gtaagaaacc gattatttat gatgtcgaaa cgctgcgtga tggtaacagc ttcagcgccc
     1081 gccgggttgc tgctattcaa aacggcaaac cgatttttta tatgacggcc tctttccagg
     1141 caccagaagc gggtttcgaa catcaaaaaa caatgccgtc cgcgccagcg cctgatggcc
     1201 tgccttcgga aacgcaaatc gcccaatcgc tggcgcacct gctgccgcca gtgcttaaag
     1261 ataaattcat ctgcgatcgt ccgctggaag tccgtccggt ggagtttcat aacccactga
     1321 aaggtcacgt cgcagaacca catcgtcagg tgtggatccg cgcaaatggt agcgtgccgg
     1381 atgacctgcg cgttcatcag tatctgctcg gttacgcttc tgatcttaac ttcctgccgg
     1441 tagctctaca gccgcacggc atcggttttc tcgaaccggg gattcagatt gccaccattg
     1501 accattccat gtggttccat cgcccgttta atttgaatga atggctgctg tatagcgtgg
     1561 agagcacctc ggcgtccagc gcacgtggct ttgtgcgcgg tgagttttat acccaggacg
     1621 gcgtactggt tgcctcgacc gttcaggaag gggtgatgcg taatcacaat taaaaaatag
     1681 ccggaggtga aaaccgtccg gctgtttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtgact gttgacatcc agcccttcta gctcctgctc ttccggtaca cgcagaccaa
     1801 ccgtcagatc cgccaatttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccagctgt accagcaact gatgacccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagcgagctg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaagccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 cccccagcag tgaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgcgg cgttaatgtg caccactgtg ccacccgcga
     2461 aatccagtgc accgtgagaa gccagcaaac cgccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt ttcagcatca
     2701 accagttaac gttgccgaag aagttgttac cctcaccaaa cgccagcgag taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcatcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcatcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aattgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccagtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcttcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aacaatggtc gataagcggt gagcaatcac caccagcgtg gtatgttcac gcacagccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccaacac gcgtgccagt gccagcagtt gcttttgtcc
     3901 gacggagaga ttattcccct gctcgcccag cggcgtataa ataccatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 acgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctgtg acttagcgaa cttaatggac gaccatcaag
     4141 gcgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtgctttt
     4201 gccactgccg gtatgcccga ccagtgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggcgat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacagca cgcctacttc
     4501 aatggtgccg ctagcggaga agccaaacag catcaacaag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctggc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacggaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacgacgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc gctacgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg caatgcggca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ttttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgct gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccgtac gctaacagac gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggagcgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca at
//
LOCUS       DAJTMK010000224         5436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTMK010000224 DAJTMK010000000
VERSION     DAJTMK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.229"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..234
                     /locus_tag="OO135_003788"
     CDS             <1..234
                     /locus_tag="OO135_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5399450.1"
                     /translation="MFNTRLYRVNFDVASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(388..2037)
                     /locus_tag="OO135_003789"
     CDS             complement(388..2037)
                     /locus_tag="OO135_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5399451.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2189..3538)
                     /gene="ghxP"
                     /locus_tag="OO135_003790"
                     /gene_synonym="yjcD"
     CDS             complement(2189..3538)
                     /gene="ghxP"
                     /locus_tag="OO135_003790"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5399452.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILHNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKKPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4083..4547)
                     /gene="soxR"
                     /locus_tag="OO135_003791"
     CDS             complement(4083..4547)
                     /gene="soxR"
                     /locus_tag="OO135_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5399453.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4633..4956
                     /gene="soxS"
                     /locus_tag="OO135_003792"
     CDS             4633..4956
                     /gene="soxS"
                     /locus_tag="OO135_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5399454.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(4959..>5436)
                     /gene="pdeC"
                     /locus_tag="OO135_003793"
     CDS             complement(4959..>5436)
                     /gene="pdeC"
                     /locus_tag="OO135_003793"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5399455.1"
                     /translation="RLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMTPIILAFRQ
                     AGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSL
                     GLKTIAEGVETEEQVNWLRKRGVRYCQGCFFAKAMPPQVFMQWMEQLPARELTRGQ"
BASE COUNT         1467 a         1517 c         1294 g         1158 t
ORIGIN      
        1 atgtttaata cccgtctcta tcgggttaat tttgatgtgg ctagcgtcca gggaatgggc
       61 atttccatac tccgtggaga ggaaaatatc cccattgata gtgataccct ggtaacacta
      121 cagaaattct ttgaagaaga ttgtacctct catactggca tgtcacaaac tgaggataat
      181 attaatgcag tcgctatgaa gattactgca gatattatgc aacacgcaga ttgaggttaa
      241 gtcgaagaac agccaatgaa ttgactttac aagagagata acttatttgt tttagaatca
      301 tgaatgttag cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt ttcagattac
      361 ctcgggcctg gttacgtcgc tttcggctta ctgattttcc tcaattagca acgcttcaag
      421 caaatcgaga tcgtgcagta atttttgcag cgtttcgttg ctgatctccc gcgtggcgcg
      481 caggtggtac agttcagcac gttcagaacg caatgccgcc agacggaagc gacgctcaag
      541 gttctcttcc tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac gacgcaggtt
      601 accaatgacg cgagaactga cctccgtaag cagctggtta tcgatattct cttcggtatc
      661 cgccgccaga cgctcctcca ttttctggat cgccacaatc gccacttctg ccgttgccgc
      721 tcgcgcaata cgttcctctt tcaattgttg cgaatgatcc gcgacttcaa tgtgttgtag
      781 cagaataggc aacatcacca cgccgacaaa cagcgagaag agaatgacgc cagccgccag
      841 gaacaccagc tcatagcgcg ccgggaagac gttaccatcc ggcaagagca gcgggataga
      901 gagcacaccg gccagagtga tcgccccacg caccccggcg aaagacgcga tcaggatttc
      961 tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc aggaagcggt tgctgaactt
     1021 tttcatcgtc cacagccagc cgaaacggac cagcatcagc gccgcatata tcagaataat
     1081 atcggtaaac agcatccaga tttcgacgtt agggtcgatt tctgccgcca tcagcgacgt
     1141 ctccagaata cccggcagct gcagacctaa cagcaggaac accatgccgt taaagacaaa
     1201 ttccagcatc gcccaggtgc tgtttgcgcg caggcgcatt gccagcggcg cacggcgcat
     1261 cacaccggag cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc cggaaacgcc
     1321 aatatgttcg gcaatcagat aagaagcgaa tggcagcaag aacagcagaa cgatctgcgt
     1381 cgcgggttca tcaccgcccc agcggctgag gaatcgcagc gagcgaccat acagccagct
     1441 caccacaaaa ccggcaagaa taccaccaat ggctactttc ataaattcga ccgtcgcacc
     1501 gccgacggtg aagatcatcg tccccatcgc cactgccacg gcaaacttca acgacaccag
     1561 gccggaggcg tcattcatca acgcttcgcc ctgcaaaatg cccatgattt ttttcgggat
     1621 gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa gtaccgccgc
     1681 cagcgcaaag gcggggatca gcggaatgcc cggcaccacc cagtaaataa ggaagccaat
     1741 gccgaccacg gtgaccacca ccagcgccag cgcgaggccg aaaatctctc gaccatgttc
     1801 gagaaattca cgggtcggcg ttttccagcc atcagcgaac agcaacggcg ggataaacaa
     1861 gactaaaaag agttcaggat caaactccac atgcaaacca aacgtcggcc acgccagtag
     1921 cgcaccgatg gcgatttgca taagcggaag cgggatctga aagggcatga cacgagtgac
     1981 caccccggac agcgagacca cgagggtcat tatcagtatg gtgaagaaga tttccatgcg
     2041 ttccctgttt gcgatgtttt tttatgatct gagaaaggcg ttatgcctga attctcattt
     2101 tttcagaata tcgcaaaagc tcacacaatg tgcggagaaa ataaaaacgg gcagccattg
     2161 ccacccgttt tcagaatccg atgaaagatt agatagccca gccacccgca tagaaggtca
     2221 ccagcgccac ggcgataacg accgtaccga tgttcaactt gcgccattcg ccggaaacca
     2281 gacgaccaat caccagagtc gcgaagccga tcatgatgcc tgttacgatg ttacaggtca
     2341 gcacgatgaa taccgccgta accagacccg ccatcgcatc aacaaaatca gcaaagtcga
     2401 ttttcgccac gttgctcagc atcagcaggc caacgtacat cagcgccgga gccgttgcat
     2461 accccggaac gaggtaagag agcggagaga ggaacagaat cagcaggaac agcacgccaa
     2521 cggtgatagc cgtcaaaccg gttttaccgc ccgccgccgt acccgccgca gactcgatgt
     2581 ataccgctgc cggagcagca cccaccaggc cagagaaaac gctgctcatg gagtcagtgg
     2641 tcagtgcttt cccaccgtcg atgatctgcc catctttatc cagcaggttc gcctggccgg
     2701 cgacggcacg gatagttccg gtggcatcaa atactgccgt catcaccagc gccagaacgc
     2761 ttggcaggac tacaggattc agcgcgccca taatgtccag gctgccaatc agtgaattgc
     2821 cgttttcatc gctcaatgaa ggcatgccga aaacgccgga gaaatggacg ttaggatcga
     2881 agatcaaacc gacaattgag ataccgataa tggtcagcag aatgccacca gggactttca
     2941 gtttttccag gccgatgatc accgccagac ctaccagtga cataatcacc gggaaggtcg
     3001 cgaaatcacc cagcgcaacg ggcagaccat caagcggttt tttaatcacc agaccaacac
     3061 cattagcggc aatgagcagc aggaacagac cgataccgat ccccgtgccg tgcgccacac
     3121 cgtgcggcaa gttgtgcaaa atccagctac ggatacccgt ggcagaaatt accgtaaaca
     3181 gcacacccat caggaacacg gcacccagcg cgacaggtac gctaatatgt tgccccagca
     3241 ccaggctgaa tgcggtaaac gccgtcaggg agatggcgca accaatcgcc aacggcagat
     3301 tagcccacag acccatcacg atagaaccga gtccggcaac cagacaggtt gcaacgaaaa
     3361 ctgccgcagg cgggaagcct gctttaccca acatacctgg aacgacgatg accgagtaga
     3421 ccatcgccag aaacgttgtt aacccggcaa ccacttcctg acggacagtg cttccacgtt
     3481 gtgaaatttt aaaccaggcg tcgagtgaac cgccggtacg cgctgatggc gtagacataa
     3541 gaaaacatcc cctgagagtt taattttcgt caagatgcgt ggcggacaat cccctgccag
     3601 cgaaacgtta tcttcctgct ccaggtttgc gacaatttac tgcgtcgcaa aaaagcaaac
     3661 gtttagctcc gcgcatacaa aacgggtggt aaaaaaagca aacgattatc cggcgatcat
     3721 agcggctttt tcaaccgaag tttgctgctt ttttctcttt tattgtcttg acgatgaaaa
     3781 aacaggcaat tgatgcgcaa acgttatcgt cggtgcgcaa gattgcagcg ttatcgagcc
     3841 gtataccgtt caggattgcg ataaaaaatc gtctgagatg acaaaaatat tgcggttaaa
     3901 aagtatactt tacactggcg aagaaacgtc ggtgacatac aattaaagca tcaacaccaa
     3961 ccggaacctc caccacgtgc tcgaatgagg tctgttgacg tcgggggaaa ccctcctgtg
     4021 taccagcggg atagagagaa agacaaagac cggaaaacaa actaaagcgc ccttgtggcg
     4081 ctttagtttt gttcatcttc cagcaagcgt gcgccggtac cttcttctcc taagcggtcg
     4141 cccgggttac gcaacgggca atcactgcgc gaaaggcagc cacaaccaat acatccgtcc
     4201 agttcgtcac gcagcgccac taaggtatga atgcgccgat ccaactcttc tcgccattgg
     4261 gacgaaagct gtttccactc tttcgcactt aacgtatgcc cttctggcaa cacgccaaac
     4321 gcttcaccaa tggtcgccag cggaatgcca atacgctgag caattttgat aattgcaaca
     4381 tatcgcaaca catcacgttt atatcgccgc tgattgccgc tgttacggat actggtaatc
     4441 aaccctttac tttcatagaa atgcagcgcc gataccgcca caccgctgcg tttcgccact
     4501 tcgccggggg ttagcagcgc tttaatgcgg ggtaatttct tttccataaa tcgctttacc
     4561 tcaagttaac ttgaggaatt atactcccca acagatgaat taacgaactg aacactgaaa
     4621 agaggcagat ttatgtccca tcagaaaatt attcaggatc ttatcgcatg gattgacgag
     4681 catattgacc agccgcttaa cattgatgta gtcgcaaaaa aatcaggcta ttcaaagtgg
     4741 tacttgcaac gaatgttccg cacggtgacg catcagacgc ttggcgatta cattcgccaa
     4801 cgtcgcctgt tactggccgc cgttgagttg cgcaccaccg agcgtccgat ttttgatatc
     4861 gcaatggacc tgggttatgt ctcgcagcag accttctccc gcgttttccg tcggcagttt
     4921 gatcgcactc ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg cgttaactcc
     4981 cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcaaaa
     5041 aaacatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg ctcctcagtt
     5101 tcgacgcctt cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc
     5161 gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt atcgattttc
     5221 aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca
     5281 tcaattgcca cttcgtaacc agcctggcgg aaagccagaa taatcggcgt cattttttcg
     5341 acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg caccgcgtat
     5401 tgctctgttt tctgattgat tcgcgctatc aaccgt
//
LOCUS       DAJTMK010000225         5423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTMK010000225 DAJTMK010000000
VERSION     DAJTMK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5423
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.230"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            222..872
                     /gene="inaA"
                     /locus_tag="OO135_003794"
                     /pseudo
     CDS             222..872
                     /gene="inaA"
                     /locus_tag="OO135_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(926..1180)
                     /gene="yfaE"
                     /locus_tag="OO135_003795"
     CDS             complement(926..1180)
                     /gene="yfaE"
                     /locus_tag="OO135_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5399456.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(1180..2310)
                     /gene="nrdB"
                     /locus_tag="OO135_003796"
     CDS             complement(1180..2310)
                     /gene="nrdB"
                     /locus_tag="OO135_003796"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5399457.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFTSNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(2457..4742)
                     /gene="nrdA"
                     /locus_tag="OO135_003797"
     CDS             complement(2457..4742)
                     /gene="nrdA"
                     /locus_tag="OO135_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5399458.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLIMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1338 a         1279 c         1413 g         1393 t
ORIGIN      
        1 aattcatcgc tgtatccgcc tgattaaatt cacattttat ctgctgggcg cactattagt
       61 ggtagtggct ggtggaatct atgcgctgca aatgaagggt aaaagcggtg tcaagctagt
      121 agtggcaaaa gaaattcatt aatacgacac gtttcattaa gattttcctc aggtaaccac
      181 ctatagtcat tgtcgttaat aaaaacgaca ggcgataggg tatggcagtt tcagcaaagt
      241 acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc
      301 gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa
      361 agcgcatgac gcatcatctg tttcattctg tacgttatcc gttcggccga ccaacgattg
      421 tccgtgaggt tgcggtaatt aaataactgg aacgggccgg tgtcatcgtg ccgaagatcg
      481 tttttggtga agcggtgaaa attgagggtg aatggcgagc gttgctggtg actgaagata
      541 tggcggggtt catcagcatt gctgactggt atgcccagca tgcagtatcg ccttattctg
      601 acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagca
      661 ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg
      721 cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga
      781 ttaatcatga cttccgccag ctggaaaaat atctggagcc aatccccaaa gcggactggg
      841 agcaggtgaa agcgtattac tacgccatgt aatcgttgta ggccagataa gacgcataag
      901 cgttgtatct ggcactacaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc
      961 aacaacaggg caaaatttcc cccggctgaa taaaggctaa cggttcggca atccagtcaa
     1021 cttgacctgc aaccaggcgt gtgcgacagg agccgcagta accttcgcga cactggtact
     1081 caaccgccac attgtgggat tccagcgctg ccagaaggga agggtgttca tcctggcaca
     1141 gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat cagagctgga agttactcaa
     1201 atcgtcggtg tccacttccg agtcaatctg cccgaccaga taagaactga cttccacttc
     1261 ctgcggagca acctgcacgt tatcagacac cagccaagtg ttgatccacg ggatcgggtt
     1321 ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta
     1381 ttcaacgtac tggcagagaa tgtctttatt cagaccaatc atcgaaccgt cgcggaacag
     1441 ataatccgcc cagtctttct cctgttgagc tgcctgaaca aacaggtcat agcactcctg
     1501 cttacactct tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc gcagcagatt
     1561 cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat
     1621 tttggcgttg ccttccatca attcgcgttc tgcaaaggca aaggaacaag caaagctgac
     1681 gtagaagcgg atcgcttcca gcgcgttaac gctcatcagg caaagataca gttttttctt
     1741 cagctcgcgc aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag
     1801 aaggtgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga tcccttccgc
     1861 acgtttctgg atctgctcgt tggtgacgat atcgtcaaac acaatagacg gatcgttaac
     1921 gatattacga atgatatgag tataggaacg ggaatgaatc gtttctgaga acgcccaggt
     1981 ttcgacccag gtttccagtt ccggaataga aataagcggc aatagcgcca cgttcggact
     2041 acgaccctga atggaatcca gcagcgtctg atatttcagg ttgctggtaa aaatgtgttt
     2101 ttcgtgctcc ggcagcgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg
     2161 acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg
     2221 atcgtagcgg gccacgttga ccggctgacc aaagaacata ggttctttga gctgatcatt
     2281 tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc tgttgggagt ttaatgccgg
     2341 ataaggcatt ttacgccgca tcctgtgcct gatgcgacgc tggcgcgtct tatcaggcct
     2401 acaaatccga gccgtaggcc ggataaggcg ttcacgctgc atccggcatc tcaatatcag
     2461 atcttacatg cgccgctttc gcagccatcg tcctggattg acggtaccag atcgtcctgc
     2521 gcgtcttcag cgccgtcacg ggtgttctga taatacagcg ttttgacccc gaatttgtag
     2581 gcggtgagca ggtctttcag caactgctgc atcggcactt ttcctgacgg gaaacgtgac
     2641 ggatcgtagt tggtgttagc agagatcgac tgatcgataa atttctgcat gatacctacc
     2701 agttgcaggt agccatcgtt acccggcatt tcccacagca gctcataggc gtcgtgcagg
     2761 tgctcgtagt ccggcaccac ctggcgcaaa ataccgtctt tcgacgcttt gatgctgacg
     2821 taaccgcgcg gcggttcaat accgttagtg gcgttagaga tctgcgaaga ggtctcggac
     2881 ggcatcaggg cagaaagcgt ggagttacgc aggccgtgcg ttttgattga ttcacgcaat
     2941 gcttcccagt cgtaatgcag cggctcatta gcgatggcat ccagatcttt cttataggta
     3001 tcgatcggca ggatcccttt cgcgtaagtg gtttcgttaa accacgggca cgcgccttgc
     3061 tctttagcca gctcattaga cgctttcagc aggtaatact gaatcgcttc gaaggtttta
     3121 tgcgtcaggt tgttggcgct gccgtcggag tagcgtttac cgtgcttcgc caggtagtaa
     3181 gcgaagttga tcacaccaat acccagcgta cgacgaccca tcgctccacg tttggcggcc
     3241 gggatcgggt aatcctgata atccagcagc gcgtcaagtg cacgaaccgc cagaattgcc
     3301 agctcttcca gttcatccag gttattaatt gcgcccaagt tgaaagcaga cagcgtacac
     3361 agcgcgattt caccgttctc gtcgttgacg tcgttcagcg gtttggtcgg cagggctatc
     3421 tccaggcaca ggttagactg acgcactggc gcgatggccg gatcaaacgg gctatgggta
     3481 ttacagtggt caacgttctg aatatagata cgaccggtag acgcacgttc ctgcattatc
     3541 agcgagaaca gctcaacggc tttcacacgc tgcttgcgga tgctgtcgtc tttctcatat
     3601 ttggtataca gacgttcaaa ctcttcctga tcggcgaaga acgcgtcgta cagccccggt
     3661 acgtcggacg ggctgaacag ggtgatatct tcacctttca gcagacgggt atacatcagt
     3721 ttgttgattt gtaccccgta gtccatatga cgcacgcggt tgccttccac accacggttg
     3781 tttttcaaca ccagcaggct ttccacttcc agatgccaca tcgggtagaa cagcgttgcc
     3841 gcaccgccgc gcacaccgcc ctgagagcag gatttcaccg ctgtctggaa atgtttgtag
     3901 aacggaatgc agccggtatg gaacgcttca ccaccgcgaa tcgggctacc cagcgcacga
     3961 atacgcccgg cgttgatgcc gatcccggca cgctgggaaa cgtatttaac aatcgcgctg
     4021 gaggtggcgt tgatggaatc caggctgtca ccgcactcga tcagtacgca ggagctgaac
     4081 tgacgagtcg gggtacgcac gccggacatg attggcgtcg gcagcgaaat tttaaatgtg
     4141 gaaaccgcgt cgtaaaaacg cttcacatat tgcaggcgcg tttcacgcgg gtagttcgag
     4201 aacaggcacg cggcaaccag aatataaagg aactgggcgc tctcatagat ttcgccggtc
     4261 acgcggttct gtaccagata tttgccttcc agctgcttaa cggcagcata agagaaggtc
     4321 atatcacggt cgtgatcgat aaacgtgtcc atctgcttga actcttcttc cgtgtagtct
     4381 tccagcagat gattatcgta tttgcccatc tcgaccattt tcaccacgtg gtcgtacagc
     4441 gcaggcggct caaactggcc gtaggctttt ttacgcaggt ggaagatcgc caggcgcgcg
     4501 gcgagatact gataatccgg cgcatcacgg gagatcaggt ctgcggcagc cttgataatg
     4561 gtttcgtgga tgtcagaggt cttgataccg tcataaaact gaatgtggga gcgcagctcg
     4621 acctgggaaa tcgaaacgtt atgcagtcct tctgccgccc aatccagaac gcgatggatt
     4681 ttgtcgagat tgatgcgctc tgtgctaccg tcgcgctttg tcaccagcag attctgattc
     4741 atgtatgtcg tacctgtttt tggaattctt tccgcaatag tttcatgcag actacctgta
     4801 gtacgggaag ctgcttagaa cactatatat agggggtatg tttgggattc actgcaagat
     4861 agtgtgaaaa tggccctctt gcaagtgcat aactttgtgg ataactcagg aaggaaaaag
     4921 tggctttcgc gcaccttagg tcagacaagg tgtccgggaa agtcaatggg aagagaaaaa
     4981 tttgttaaaa ataactgttc ggcgatttct tgaacggcag aataggttgg ataaattgat
     5041 atagatggtt tattaatctt tttgctaatg attgcaaaaa ttgatctaag cacgagtcat
     5101 cacataatct gatgtatcag ttgtggtcaa tatataagcg aatgtaagcg attgttgatg
     5161 tcgaaatggt tgttttctat tcggttattg ttaaatgcga agccattttg ttaatttacc
     5221 ttatattata aaaaaattat tcattacttt aatttagttt aaatataact ggtatccctt
     5281 aaggttatta acttgaatta tgacgcactg atattattca tcaaataata acaaaatagc
     5341 cattgcatcg ggttggacaa tttacaaaag aaagataacc catattgtat aatgcgttta
     5401 aatatgcgga ttatctttct acg
//
LOCUS       DAJTMK010000226         5405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTMK010000226 DAJTMK010000000
VERSION     DAJTMK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.231"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            30..1382
                     /locus_tag="OO135_003798"
     CDS             30..1382
                     /locus_tag="OO135_003798"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5399459.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVQTGKSINPEFNIGCMIAMYPIY
                     PLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLDITPEDNAILAS
                     GCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQIDPAGLRYSLN
                     WFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKKAVVEDGVDLIG
                     YTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWYRDLIANNGENI
                     "
     gene            complement(1439..1657)
                     /gene="marB"
                     /locus_tag="OO135_003799"
     CDS             complement(1439..1657)
                     /gene="marB"
                     /locus_tag="OO135_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5399460.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1689..2072)
                     /gene="marA"
                     /locus_tag="OO135_003800"
     CDS             complement(1689..2072)
                     /gene="marA"
                     /locus_tag="OO135_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5399461.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2092..2526)
                     /gene="marR"
                     /locus_tag="OO135_003801"
     CDS             complement(2092..2526)
                     /gene="marR"
                     /locus_tag="OO135_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5399462.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2738..3379
                     /gene="marC"
                     /locus_tag="OO135_003802"
     CDS             2738..3379
                     /gene="marC"
                     /locus_tag="OO135_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5399463.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRNSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINDH"
     gene            complement(3417..4606)
                     /gene="ydeA"
                     /locus_tag="OO135_003803"
                     /pseudo
     CDS             complement(3417..4606)
                     /gene="ydeA"
                     /locus_tag="OO135_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
     gene            complement(4756..>5405)
                     /gene="yneK"
                     /locus_tag="OO135_003804"
     CDS             complement(4756..>5405)
                     /gene="yneK"
                     /locus_tag="OO135_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5399464.1"
                     /translation="HRALSEHKTLSISWSHYPPHIEPLPPDHHTAADYLRQFDLLVLN
                     FISRGNFVILPRLWSNSEVHRWFVNKDPNLITSILDITDGELKEDLLQSLMDSLGSNK
                     HVLPEVCICFLSLLAEQESPHFQDFFLFFANMLLHYHQFMNPNESDLNDMLMPASLSD
                     DKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
BASE COUNT         1455 a         1205 c         1333 g         1412 t
ORIGIN      
        1 tatggggcgg cgcggtagcc gcgcatcagt tggaaggtgg ctggaatgaa ggaggaaaag
       61 gcatcagtat cgctgatgtg atgactgctg gcgctcacgg ggtgccgcgt gaagtgacag
      121 aaggcgttat cgacgggctt aattatccca atcatgaagc aattgatttt tatcatcgct
      181 ataaaacaga tattcagtta tttgccgaga tgggattcaa atgctttcga acttccattg
      241 cctggacacg aatctttccg caaggtgacg aacaggagcc gaatgaagag ggtttacaat
      301 tttatgatga tctgttcgat gaatgcctta agcagggaat ggaacctgtg gtgacgcttt
      361 cacattttga gatgccttat catctggtga caaaatatgg tggctggcga aaccgtaaac
      421 tgatcgactt ttttatccgc ttcgcatcaa cggttttcac gcgctataaa gaaaaagtaa
      481 agtactggat gacgtttaac gaaatcaata atcaggtgaa tttcagcgaa agcctgtgtc
      541 catttactaa ttccggtatc ttgtattcgc cagaggaaga tatcaatgag cgcgaacaaa
      601 taatgtacca ggcggtacag actggaaaat cgatcaatcc tgaatttaac attggctgta
      661 tgatcgccat gtatcccatc tatcctctga cgtgtgtacc caacgatatg atgatggcca
      721 cgaaagcaat gcatcgtcgt tactggttta ctgatgttca tgctcgtgga tattatccgc
      781 aacatatgct gaattacttt gccaggaaag gattcaacct cgatatcaca ccagaagata
      841 acgcgattct tgccagtggt tgtgtcgact ttatcggctt tagctactac atgtctttta
      901 cgacacaatt ttcgcccgat aacccgcaac tggattatgt tgaaccacga gatttggtca
      961 gcaaccctta tatcgataca tccgaatggg gatggcaaat tgatccggca gggctacgtt
     1021 attcactcaa ctggttctgg gatcatttcc agttgccgct gtttattgtc gaaaatggat
     1081 ttggtgcggt tgaccagaga caagctgacg gcacggtgaa cgatcactat cgcattgatt
     1141 actttgcttc ccatattcgg gaaatgaaaa aagccgtagt tgaagatggt gttgacttaa
     1201 ttggctacac cccgtggggc tgcattgacc tggtttctgc cggaacaggg gaaatgaaaa
     1261 agcgctacgg aatgatttat gtcgataaag acaacgaagg gaagggaacg ctggaacgga
     1321 tacgtaaagc gtcgttttac tggtatcggg atctcatcgc caacaatggc gaaaatattt
     1381 gagacttgag gaagatatta cggagatgtc aggctctgtg atcagagcct gacaataact
     1441 acatagcgtg ttgattataa tagggcacgc cgagcgcatc cgacttatca ctgccagtac
     1501 ccatgtgatt taaatcaaac ggtggttgtt cctgcgatgg gggaacaacc acgacattgg
     1561 cacaagaagt aacaactggc tgcgtggttt gttccgcaac gccctgcgcg gaaaagagaa
     1621 taagcgcagc tgctattgcg gatgaaagtg gtttcatgat tgcctcagtg acgttgtcac
     1681 gttatcaact agctattgta atgatttaat ggatgtaaaa agcgcgattc gccctgcata
     1741 ttggtcatcc ggtatttatg tggcggaaca tcaaagtaat ttttgaaggt tcgggtcaga
     1801 gtttgttgcg actcgaagcc atatcgttct gccagataga gtatcggctc gttactttcc
     1861 ttcagctttt gcgcgatttc cgtcatctta cggctgcgga tgtattggcc taatgaatga
     1921 ccggtttctt ttttaaacat ccgttgcagg tgccatttag agtaacctga acgctctgac
     1981 actttctcca gtgacagtgg cgattccagg ttgtcctcga tccagtccaa aatgctatga
     2041 atggtaatag cgtcagtatt gcgtctggac atcgtcatac ctcttttttg tttacggcag
     2101 gactttctta agcaaatgct caagtgttgc cacttcgtcc gccgtcaggt tttttgttaa
     2161 ttcttgatgc aggtcctggc caactaattg atggcattgt tcgcatattg ccgcgccgct
     2221 ggtggtaagt tttaccagta cgccgcgctt atcattcggg ttcggcaacc tttcaaccca
     2281 gcctttacag accaggcgat ccagcatacg ggtcagtgct ccaaggtcga cagacaacac
     2341 ttttttcagt tcaaccggag taatacacgc cgcgcagcgg atagagcaga gcaccttaaa
     2401 ctgtgccgcg gtaatatcca gcggagacag atactcgtta agcagacgat ctttcttctg
     2461 attaaccata tggattaagc gacccaatgg aataatttca ttgaacagat cgctggtact
     2521 tttcacatga gttgccctgg caagtaatta gtcgcagggg gtaatattgc ccaggcaagt
     2581 ataagtcaaa taaatgaatt gaccgatgcc acgttttgct aaatcggttc aagttttggt
     2641 caatgcgatg caaggttagc taaagagtga cactttatcc ttgtttaccc cttatacttt
     2701 tcgctgataa cccagataca ctggataaca accaccaatg ttagatttgt ttaaagcaat
     2761 tggcctgggg ctggtggtgt tactgccgtt agctaaccca ttaacaactg tcgcgttatt
     2821 tcttggcctg gcaggcaaca tgaacagtgc cgaacgtaat cgtcagtcgt tgatggcctc
     2881 ggtgtacgta tttgccatca tgatggtggc gtattacgcc gggcaactgg tgatggatac
     2941 atttggcatt tcgattcccg gtctgcgaat tgcaggcggc ttaatcgtcg cgtttatcgg
     3001 ttttcggatg ctctttccgc aacagaaagc gattgattca ccggaggcga aaagcaagtc
     3061 ggaagagctg gaagatgaac ccagtgccaa tattgccttt gtgccgctag caatgccaag
     3121 taccgctggt ccgggaacca tagcgatgat tattagctcg gcgtccacag tgcgtcagag
     3181 ttcaaccttc gctgattggg tactgatggt tgcgccgccg ctgatctttt tcctggtggc
     3241 ggttattttg tggggaagcc tacgcaactc cggcgcaatt atgcggctgg tgggcaaggg
     3301 tggcattgaa gcgatctccc gtctgatggg attcctgctg gtatgtatgg gtgtgcagtt
     3361 tattattaac gatcattaaa acgtaccatt aaaaaaggcc cgaatgggcc tttcaactat
     3421 tgcgtctgtt cttcgagtgt cactgccagc ggcgaaatat aatgattgac catattaacg
     3481 cggcaaaagc aggcaccgcg cccacataac caatcatcga cattgaccag tgcaaactca
     3541 cctgattacc taccaacgca cccgccccga ttccaatatt aaatatgccg gagaatagcg
     3601 ccatcgcgac gtcggtagca tctggtgcca gcgccagcac tttaacctgc ataccaagcc
     3661 cgatgatcat catcgcgatc ccccagaaaa tactcagcac cccgaggtgt atttcactgt
     3721 tcgccgcagg tagcagcagt gccagacaca ccagcaacag cgcaatcgcc gtactcacca
     3781 acgcagacgc atactgatta cccagtttac cgaaaatcac gctgccaata atgcccgcac
     3841 caccgagtaa taacagtaat gccgtggcaa agttggcgct gaatcccgca atgttttgca
     3901 caaaaggctc gatatagctg tatgccgtgt aatgggcggt gacaaccacc acagttaaca
     3961 aataaatgct catcaatgcc gggcggcgga acaatagcgg gaggcttttc aatgaaccgg
     4021 aatgctcact gggcagtaag ggaagtaact taatcaggca caaaagggtg ataagcgccc
     4081 caataccaat cgcgaagaag gtcattcgcc aaccgaaata ctgccccaca atgcgcccga
     4141 gaggtaaacc taagaccatc gccagtgctg taccggtggc aattaaactc aaggcctgtg
     4201 cccgcttccc ggccggagcc atacggatcg ccagagacgc cgtaatcgac cagaaaatcg
     4261 catgtgcaaa agccacacca atgcgactga tcaccagaac ggtaaagctc cacgataaaa
     4321 acgacagtac gtggctggca ataaacacca caaacaggca gatcagtaat ttgcgccgtt
     4381 caacctgact ggtcattaac ataaaaggca atgacattag cgctactacc catgcgtaaa
     4441 tggtcaacat gatgccgacc tgagcggttt gcatgtgaaa actttgcgca atgtcagaga
     4501 gcaggccaac agggacaaat tcggtggtgt tgaagatgaa ggcggcgact gccagcgtaa
     4561 cgacccgtag ccacgccact ttgcgggaaa cagtgtttgt tgtcataaca gattatcggg
     4621 tgagttgcga aaaagtgatg aaatgatctt aaaacggtta atgatgtgaa tacaatgcat
     4681 ttgtgatctg gatcgcgttt tccgtttctc tgacgctgat tctgttccgc gtcagagagt
     4741 cggtaacatc acggtttaat gacgttcagg gaggtctggc gttttggcgg ttgcgggtat
     4801 aggtggtctg acaggggagc gaggcctgtt tttcaccagg tcttcgtgag taacttttgg
     4861 cggcgtttca ttttttacaa acagtttgag ggtcctgcgc gccatatgct tgataatttt
     4921 atcatcactt aatgatgctg gcatcaacat gtcattcaga tcactttcat tgggattcat
     4981 aaattggtga tagtgcaata acatattggc gaaaaataaa aaaaagtcct gaaaatgagg
     5041 agattcttgt tctgctaaaa gggataaaaa gcagatgcag acttcgggta gtacatgttt
     5101 gttagaacct aatgaatcca tcaggctttg caataaatcc tcttttaatt cgccgtctgt
     5161 tatgtcgaga atggaggtga tcaagttagg atctttattg acgaaccatc tgtgaacctc
     5221 agagttactc cataatcggg ggagtatgac aaaattaccg cgagagataa aattcaggac
     5281 aagcaaatca aactggcgta agtaatctgc agcggtatgg tgatccggag gcaggggttc
     5341 aatatgtggt gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg
     5401 atgac
//
LOCUS       DAJTMK010000227         5360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTMK010000227 DAJTMK010000000
VERSION     DAJTMK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.232"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(912..1853)
                     /gene="fabY"
                     /locus_tag="OO135_003805"
                     /gene_synonym="yiiD"
     CDS             complement(912..1853)
                     /gene="fabY"
                     /locus_tag="OO135_003805"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5399465.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1898..2335)
                     /gene="dtd"
                     /locus_tag="OO135_003806"
     CDS             complement(1898..2335)
                     /gene="dtd"
                     /locus_tag="OO135_003806"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5399466.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2332..3204)
                     /locus_tag="OO135_003807"
     CDS             complement(2332..3204)
                     /locus_tag="OO135_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5399467.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(3198..3797)
                     /gene="yihX"
                     /locus_tag="OO135_003808"
     CDS             complement(3198..3797)
                     /gene="yihX"
                     /locus_tag="OO135_003808"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5399468.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3938..4825)
                     /locus_tag="OO135_003809"
     CDS             complement(3938..4825)
                     /locus_tag="OO135_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5399469.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4815..>5360)
                     /locus_tag="OO135_003810"
     CDS             complement(4815..>5360)
                     /locus_tag="OO135_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5399470.1"
                     /translation="FGIVYISSQLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQ
                     KSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFG
                     TKVRYTGLSFAYQFSAIVGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLI
                     EPTDEQETASYRYIREQSHEN"
BASE COUNT         1446 a         1354 c         1253 g         1307 t
ORIGIN      
        1 tccgctattg ccagtacacc tcattctgat aaatgaagaa aaaccgtatt catcgccatt
       61 accaccacct tacctatatt actcattaat gtatgtgcga aaattacgcc ggaaaatcat
      121 taaaaaaaag cggatttttg catgttgttt tgtttttgcg aggctttttc cagaaataat
      181 aagagtgttg cagttgtttc atttattact caaaaatggt ttagttatcg cccaggatga
      241 attatgggtg ctatgaatat attatattca gagatattta tttgcataat ctgaatggtg
      301 tagccgaaaa acgtcagcat cctaacagca ccaggattta aggtgaaatt aatctttcat
      361 cacagaccgg tcagtactgc caccaaccgg tctttacttg ctcatcgtta taattttgtt
      421 ggtaacagtt cgggaaagtt gtttttctaa attaacaaca gaataacttt cttgtttaat
      481 gagattaata ttggcaacca ttatagcctc cttcccacgg tttaagagca aacatacaac
      541 ataatgattc ataacataca gagcagtaaa tgaatgatac tgacaatgat gccagcccat
      601 tatctttgca taatgtttgt gagctataga ttttaaaaat atttatgagg aaagtatggc
      661 accagaggag gttttgacta tcaaaaatcg ctcaaacctg aactaagatg atgagttaag
      721 cattatctgc gataattgcc actcagctct acaaataata tttaaatgct cctgaacagt
      781 gatgtagcaa gacacgtatg gtgccaacga agaataaaaa tttatagagt tcttattcaa
      841 tattaccggc atagacaggg aatgatgtaa atgaccggaa tgagagaggc agcaccgccc
      901 ctctccataa gctactcttc ttcgttcccg ccctcttcat acgggccaaa tggcttcgcg
      961 ggcagaacga tatacgtgcc ttcaaacact gcacccggcg tctcgtcgcc aaagatttca
     1021 acttgcatct gcacccgtgc ttttcgtccg cgcgccagac ggtcgagatc gccgcttaag
     1081 gcaccaaggt cggctaccgc atgaggttta ccgctaatcg gcttgctgta gcggatatgc
     1141 gcatcggcca gaataatcgt tccgccgagg tggcgttcgc gcagcatcag ccagataagc
     1201 ccccaaccgg tgagcgttgc cagcgagaat aaactcccgg caaacagcgt atggtgcgga
     1261 ttctgattac cggtttccgg catggtggtg ataaattttt gcccggtata ttgctgaatg
     1321 cgcacgccca ttttttcact aagcgggatg tgttcgtacc acgcctgttg cagctgcgcg
     1381 caccagtcgc cgcgatgcaa aatatcatcc agagtggcaa caggtttaat catcaaaaaa
     1441 tggcgaatcg gtgtggtggt tggtgtggtg atttctccct gattaacaaa ccccagcttg
     1501 gcgaaaaact ccaccgcgtc ttcacgggcg ctacaggtca cgcgcttaac gccttcctga
     1561 cgcgccaccg actccagggt catcgccatc aacgtcccca gccctttgtc ctgcacgtcg
     1621 ggatgaacgg ccataaagcg aatggacgct tcattgtcgg cattaatata cagtcggcct
     1681 accgccacca gattcccctg ctcgtcgacg accatctgat gatgcgccat cgcatcccac
     1741 gcgtcgcgtt ccgaaccttt tggttgatgc aggggcttac gcaacatttc ccagcgaaac
     1801 tgatagtaac gctctaactc ttcttctgtt tgtggaaccc gaaggtgata catagcgata
     1861 ctctctcttg tttcccgtaa caaccctgga agctggctca tacctgcaac cagaatgtca
     1921 cggggccatc attgaccagc gatacctgca tatcggcagc gaagcgtcct gtttgcgtgt
     1981 tcatctcttg ctggcggcag cgttcgacga aatagtcata taacgcctct gcgcgatccg
     2041 gtgatgcgcc tttggagaaa cttgggcgca tcccccgttc ggtatctgcg gcgagggtaa
     2101 actgggaaac caccagtaca ctgccgcccg cctgttgcac gttgagattc atcttgcctt
     2161 cggcatcgct aaagatgcgg tagccgagca cacgctcgca cagacggttt gctttctgtt
     2221 cgtcgtcatc cttttcgaca cccaataaca ccaaaagtcc cgcgccaatt tcgcccgtca
     2281 cttctccctc cacggtgacg ctggcacggg ttacgcgttg aattaatgca atcatggttc
     2341 gtcgtcttct tcttgttcag ctgcttgttt tagtttgcgg tattccccga gagtgacagt
     2401 aatttccgcg ccaagcaaga cgatacacca tgtccagtag acccaaacaa agagaatggg
     2461 gatcaccgcc agcacaccgt aaatgagctg atatgacggg aacatggtga tataaagcgc
     2521 gaaacctttc tttcctgctt cgaacaggag tgcggcgaca aacgcgccga caatcgcgtc
     2581 gcggttaggt acgcggatgg taggaacaat gctgtacagc aaccagaagg agatccacga
     2641 caacagcaac ggaaaaatac gcagcacgtt gtcgatgaca gtattgagat cgctcgccca
     2701 gcgcagggag agcaaatagg aactgatcgc cagactggcc cctgccagca gcggccccag
     2761 cgttaaaatc atccagtaca cggcgaacga gtaaattttt ggtcgcgctc gtttactgcg
     2821 ccagatggta ttcaacgcgc tatcgatgga gtacatcaac aataacgcgg tgacgatcag
     2881 cccgcacgcc ccaacggcgg tcatcttgtt ggaattggca acaaattgtt cgatataccg
     2941 ctgaataaca tcgccagtag caggcagaaa gttggcaaaa ataaagtgac gtaactgaat
     3001 gctgacgtcg gaaaacatgg gaaaagcggc gaaaagcgca aaaacaacgg caaccagcgg
     3061 cactaatgag agcaacgaca cataggcaag gttacctgcc agggttgtca tgttgtcctc
     3121 atcaatacgt tgccagagta gttttagcca ggcccatagt ggacgggtac ggtgcctggc
     3181 tttgtcctga atggttttta gcataacacc ttcgcgaaat agtccgggat ggtggtttta
     3241 tctttcacca gaatactggt aatgcccagc tgattggctc cttctatatt atcggcgtta
     3301 tcgtcgaaaa agaccgtatc gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa
     3361 attcgtgctt caggtttgcg catccccaga tcttgcgaca gatagatatg gtcagcagca
     3421 tcacgaattt ccgggtattc ttccggccag aaggtggtat gcaggcggtt ggtattggaa
     3481 agcaccacca cgcgatgccc ctgctcacgc agtttatgca tgatggcgat cacttccggg
     3541 cgcagcgcaa caaacaccgc ctgccagccg tgagagaact gctcgtagct tagcggtaga
     3601 gccatctcat gacacagcgc ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc
     3661 tgatgaaacg cctcccccat atgaaaactc ttcttaagcg tagccagtgg aacacgcgtt
     3721 aaatcgctcc aggctcccag cacacggtta aagtcgatat cgacaatcac attacctaaa
     3781 tcaaagatat agagcatttt tgcctccttt cgcgccacaa gaaattaact gtagcgggaa
     3841 aggatgggtt tggctatgtg gcaataaggg taatcttgtg atgctgattg cgaaaaagca
     3901 gagtaatttc actggctttt tcgcaataga tgaatggtta agattcaggt gtaaattgcc
     3961 aacgagtttc gatgaccagc ggcctatctg gtgtcaccaa ttccggcgtt agatttattg
     4021 cgttaggtgc ctgagtcagc gggttaatac aggtggcatc gggctgttta tcaaatacca
     4081 ccagactgtt tgcagaagag atcatggtca tcgcgagttt accaggccac agtaacttaa
     4141 ccttcacccc gttataaaaa ccaaaacagt catcccaggg accggcttga ggagagatac
     4201 gatttccagt tggcagttca tcactacccg cttcttcttg ccagtccgca tcaaaaagca
     4261 cctgcaacga ttctgtattt tgcggagtca gctttttcgc aaaccacgga tgccagccag
     4321 cggaggccgg gaatgtatcg gcatatgaat ggacttccag ttgcaaaacc agcgcatcgt
     4381 tctccagcaa aaaagtctga atcacttccc cttgccaggg ccacggtgaa gccaggggca
     4441 tacgtaaagt cagggaatct gctgttttgt cgatgatttc ccacgttgag taacaagcca
     4501 taccatgcaa cgcatgtggt ggtttattgg cgggtaaaga ataacattgc ccaccagcat
     4561 tcagcgtcgc attccccagt cgccctgccc acggcaccat tgggaaacag ccatactgaa
     4621 aagctctgcg ctgcggctgc cattggcgta atacttcaga accaaatgcc tttaacgagg
     4681 ttatcctggc accatcatgc ggataaatct gcaggtgaat attgcctttt tccagcgtca
     4741 ctaactctcc tacaggccca ggccatggcg cgtcaaacgc ctgttgtagc gccaggtaat
     4801 gctcgttttt tcgttcagtt ttcatgagat tgttccctga tgtagcggta agaagcggtt
     4861 tcttgttcat cggtcggctc aatcagccag acgcaaacaa aggaaagaac tgagacagca
     4921 aataaaaaca gagcgacata ccacggtttt cctcccccta gcgccagcaa acttgaggcg
     4981 ataagggggc ttagtccgcc aacaatcgcg gaaaattgat aagcaaaaga aaggccggta
     5041 tatcgtactt tggtaccgaa catgcgggtg aaaagtgttg gctgcacggc aaacatcatc
     5101 gtcggaccta agttgtagcc gaggatcata ctgcaccaga tgatgagcgt acttttgcta
     5161 tccagtaata agaagaaagg aaacgcgaac aggacacaaa atcctgctcc cgacaaatac
     5221 agactttttt gacctatacg atctgataac catccaacca atggacaact gaaaataccg
     5281 atcgccgagg caatcaacat acccgtcagg ggaatatctc gcgacaatgc taattggctg
     5341 gaaatataga caataccaaa
//
LOCUS       DAJTMK010000228         5245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTMK010000228 DAJTMK010000000
VERSION     DAJTMK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.233"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(34..474)
                     /locus_tag="OO135_003811"
     CDS             complement(34..474)
                     /locus_tag="OO135_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5399471.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            692..1081
                     /locus_tag="OO135_003812"
     CDS             692..1081
                     /locus_tag="OO135_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399472.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            1125..1529
                     /locus_tag="OO135_003813"
                     /pseudo
     CDS             1125..1529
                     /locus_tag="OO135_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(1636..2472)
                     /locus_tag="OO135_003814"
     CDS             complement(1636..2472)
                     /locus_tag="OO135_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5399473.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSSAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(2472..3275)
                     /locus_tag="OO135_003815"
                     /note="aph(3'')-Ib"
     CDS             complement(2472..3275)
                     /locus_tag="OO135_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5399474.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(3336..4151)
                     /gene="sul2"
                     /locus_tag="OO135_003816"
     CDS             complement(3336..4151)
                     /gene="sul2"
                     /locus_tag="OO135_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5399475.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT         1219 a         1372 c         1428 g         1226 t
ORIGIN      
        1 agatgttatg gtaaatcaga gtcacctgca ctgttactct gaaccggagc gaaacagccg
       61 cgaccagaat ccccgctttt tttcctgtgc cagcgcgtca gccagacggc taatttcggc
      121 ctgtagttgt tccctctcct catttcgcag gcggttttct gcttcccgcg ccatcttgtc
      181 ttcaagcatt agggtaacag tctgctgcag agcgtccagt ttctgctgaa tgacgtctag
      241 ctgagcattt tccgcctggc taacgacaga tttggagtgt ccctccttgt gcctttttgg
      301 tgtctcagaa gtgcgcagct cgccataggc ccgaatcagt tcactggtct cgaactcccg
      361 gcgatcattt tttcctgtgc gatagctgac aaggccttta gccatgtcac gatataattg
      421 cgagcgcgac tttcctgtta atttcagtgt ctcttgaact gtgtgccatg ccataaaagt
      481 gacagtgtct cctgtgtgta ccgtattgta acagcagaac atattactgt tttaacacaa
      541 aaggtctgag gctgcccgtg aggcgtgaaa aatacacaaa aaacctgttg acggtgtggt
      601 tttgacgggc gtagtatttc gaatactgta tataaacaca ttgttataaa tacagttttt
      661 gctgttttta aaagtcaaag aggataattt tatgagtaca gagttaaata gcggtactgt
      721 cggtggtttt ggtcacatgg gaccaaatga cccctacaat gatggtcata gcggctctgg
      781 aagttcggga ggtagtttta acggtggtgg taatggtggc catgataata gtagtggttc
      841 atcttatact gcatcctttg gtctcatcac tggccgacac ccgataactg tcccaggtac
      901 gaagaatatt aattctgtcg atctttctgc tgtcattaat aacattatac atcatgaagg
      961 tgcacgcaat aacatgtacc tagatactgc gaacaatgta agagtgggag tgacgggcac
     1021 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     1081 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     1141 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     1201 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     1261 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc
     1321 ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg
     1381 ctttcagctg taagcgtcgc ggtttctgcc cgagctgtgg ggcgcggcgg atggccgaaa
     1441 gtgccgcctt gctggttgat gaagtactgc ctgaacaacc catgcgtcag tgggtgttga
     1501 gcttcccgtt tcagctgcgt ttcctgtttg gggtcgtttg cgggaagggg cggaatccta
     1561 cgctaaggct ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga
     1621 gaagtcgctt gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc
     1681 tagatcgcgt tgctcctctt ctccatccgc gttccaagct gaggaaaggc acccataagc
     1741 gtacgcctgg tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc
     1801 catctgtgca atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa
     1861 catattggcg gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca
     1921 gccgcgactg gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc
     1981 cgaggcattg ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca
     2041 acctgcgttt tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg
     2101 gagaagggca gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc
     2161 tgcaatttcg gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc
     2221 cccggcatat tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct
     2281 cccattgcgc cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg
     2341 tttcaatccc ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct
     2401 ggaaaaggtg tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg
     2461 cggcatgaac atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg
     2521 cggtcggggg cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca
     2581 tctggcgctg cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga
     2641 tctgctgttc cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc
     2701 accatgaagt tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg
     2761 tcgagccgca ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc
     2821 ttgtcctcgt ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg
     2881 cgtccgaaca ttcgcgacag cctgcgctca aacggacatt gatcaaccga taggctgtga
     2941 acagcgccaa gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga
     3001 tcagccgccg gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc
     3061 tgccagttga tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca
     3121 cgctctccag cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg
     3181 tcaccacgtc gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa
     3241 tgcgattcac caaaaaaaat attagttcga ttcaatggag gttccttcag ttttctgatg
     3301 aagcgcgaat atagagaaat atcccgaatg tgcagttaac gaattcttgc ggtttctttc
     3361 agcgccgcca ataccgccag cccgtcgcgc aaggggcgcg gctcgtgtgt gcggatgaag
     3421 tcagctccac ctgcggcggc ggcaagctct gcagcgagtg tcgcggcccc gacatccccc
     3481 ggaccacggc ctgtgagcgc gcgcagaaag gatttgcgcg aaacagacag aagcaccggc
     3541 aaatcgaagc gcagccgcaa ttcatcgaac cgcgccagca ccgagagcga ggtttcggga
     3601 gcagccccca gaaaaaaccc catgccggga tcaaggacaa ggcggttgcg tttgataccg
     3661 gcacccgtca gcgccgcgat gcgcgcgtca aagaacgccg caatgtgatc catgatgtcg
     3721 ccagcgggtg cctcgcgccg atctgcctgc ccgtcttgca ccgaatgcat aacgacgagt
     3781 ttggcagatg atttcgccaa ttgcggatag aacgcagcgt ctggaaaacc gcgaatatca
     3841 ttgagatagg ccacaccacg cgacaaggca taggcttgcg tcgcgggttg ataactgtcg
     3901 agcgagacgg gaatgccatc tgccttgagc gcgtccagca ccggcgcgat acgcgcgatt
     3961 tctgtgtcgg acgaaacagg cgcggcgtcg ggattgctgg atgccggacc gaggtcgatc
     4021 acatctgccc cctcggccat cagcttacgc gcctgcgcaa tggctgcgtc tggcgccaga
     4081 taccggcctc catcggagaa actgtccgag gttatgttga cgatgccgaa aatgatgagc
     4141 gatttattca tgggggcttc tataataata ataatcgagc atgagtctca tacggatgct
     4201 cgggtcgaaa gggaatcccc aggcgagtaa cctgtttgcg gtgatccgtt tcatgatgat
     4261 gtatctgtag cattttggaa ataaatatat catctctcaa taagtagata tatttatctt
     4321 ttttgcttct ttggtgctgc aagtcaccaa tacgagggaa acctgttacg gagcggttta
     4381 accgtaatca acagtgactg gtgtcatgtt cggaagatta attgatagta atctacaaag
     4441 gatcttcttg agatcctttt caatgaatcg taatctcttg ctctgtaaac gaaaaaaccg
     4501 cctggggagg cggtttgttc gaaggttaag tcagttgggg aactgcttaa cccggtaaca
     4561 gggttcacga gaccgccatt accaaaactg ttctttcagt gtagccgcag tcaggcctct
     4621 acttcaagac ctcttaatct atcttctcgt gcactcctgt tactaatggc tgctgccagt
     4681 ggcgttttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga caaggcgcag
     4741 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgccttcacg
     4801 gaactgagtg tcaggcgtgg aatgagaaac cgcggccata acagcggaat gacaccggta
     4861 aaccgaaagg caggaacagg agagcgcacg agggagctac cagggggaaa cgcctgggat
     4921 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttt gcgatgcttg
     4981 tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatctct tctttgctaa
     5041 gtgtcttcct ggtatcttcc aggaaatttc cgtccccgtc cgtaagccgg taccgctcgc
     5101 cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tctcctgtat
     5161 cgcatattct gctgacgcgc cggtgctgcc ttttttctcc tgccacatga agcacttcac
     5221 tgattttcgc aatcgtggca acata
//
LOCUS       DAJTMK010000229         5203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTMK010000229 DAJTMK010000000
VERSION     DAJTMK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.234"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..121
                     /locus_tag="OO135_003817"
     CDS             <1..121
                     /locus_tag="OO135_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR5399476.1"
                     /translation="SDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(171..1919)
                     /locus_tag="OO135_003818"
     CDS             complement(171..1919)
                     /locus_tag="OO135_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR5399477.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADETQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1919..2989)
                     /locus_tag="OO135_003819"
     CDS             complement(1919..2989)
                     /locus_tag="OO135_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5399478.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            complement(2979..4421)
                     /locus_tag="OO135_003820"
     CDS             complement(2979..4421)
                     /locus_tag="OO135_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5399479.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFNFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTACFSLLEK
                     HIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAIPYT
                     LAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLAIGL
                     ALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4432..4878)
                     /locus_tag="OO135_003821"
     CDS             complement(4432..4878)
                     /locus_tag="OO135_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5399480.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1313 a         1343 c         1313 g         1234 t
ORIGIN      
        1 ctctgactgg gataaagagc aacttcagga cgcaaactca agctggatgg tcgaagactc
       61 tttcccgaga gcattacgcg agtacaacga aatggttgat gactataata gcctgcgtta
      121 aacgtttttg atgataaatg cagtgtcgga tgcggcgcat ccgacacagt tcaccccttg
      181 tgaagtaact ccataaatgc ctcatatcct gttaacccgg caataacctc tgccggatgc
      241 tgacacaata tgcttgccag atagctaaat atttttagct catgacgcgc atcggcggcg
      301 gcgcaggcga tcaagacatg gttaatgact tcgttattca cctcaactgg ttgggcgagg
      361 gtaataaaaa atccacgaaa acgtcgctcc tgttcgctcc agcaatgtgg gatggcgagg
      421 cgattaacaa tcaggttttc accttcgcct tcgcgggcga tgatgcgttg tgtttcatcg
      481 gcggtaatat ggtgttgtgc gactaattgc gcacaaattt gccgggtaat atgttgccag
      541 ctttcaccgc gtacattcga ataatgaaaa cttccggggg cagagaaaaa acgctccggt
      601 tgctggcgaa taaatgccgt cgccagaaaa tgttttattt gttcgatacc ggcagccgta
      661 atgatatttt ttacggtgat gtaattattt accgcgtctt ccagtaaata atggctattg
      721 ttaatgatca ataacggctc aatctcttcg cgaatggcaa caagttcgct taagctacgg
      781 gcaataatta cccgacaatt taaaacatcg cgctcaatgg cgagctgatt aatagtggca
      841 atcgcgttct ggtccgagag caaaatgatc ggctggcgtt cgttttgatg ccgctccagc
      901 gcacaggcaa aatacaaacc gatcagatcg ctgtcgaaaa ggggaatatc gagctgttca
      961 cgcagtagcg taataaagtg cagactcata tcaaaggccg caggccaggc tgctttcagg
     1021 ttattcatgc tactttggcg gtgctcagca atccacaccg gtgcggcaag gcaacgtaat
     1081 aaatgcccgg taatattctc aaccaactgc gtatcaataa tattcaatga cgcgatgcct
     1141 ggcacccttt gcagcaaccc cagcacatga tcggctgaaa ttacctgcgc ttgctgatgc
     1201 tgtttctcta tcaaacctgt cagggttctt acagcgttat caccaagaaa catgccgctt
     1261 tgttcaacga tttgttttat ctcgtcaccg ggatattgcg gccactcatc ggtcagttga
     1321 ttacgcaggg cgtaaatcgc cagaatcagg ctggagagat aatcaccctg catgagcggc
     1381 cagcggtgtt ggttgtcgca ggccgtggat aaatgctgaa ggttgtctcg tgttacgccc
     1441 gccagcggaa ttaaaaacgg atctttgcgc agcaagttcg ccagcaagat aacgcgcttc
     1501 tcttctgtct catcaatgaa atggcccaaa ccggggcggc tggccaggca acaagcgcgt
     1561 tcataacgct gttttaaccg gggcagacgc tctgcgaccc acgtttctgg caaattaagc
     1621 gccgaggcga gttgcgcacg gggagtaaag gtattcagta ataacagtgc cagcagccga
     1681 tagtcgttat cgtgcttttg cagcaactga aaaaagctgc ggcgctcgaa gatttccagc
     1741 tgatagcccg cactaccact ggcggaaatg cgggctttgc cgttaagggt aaagttgaga
     1801 tagtcaatat cacgcaggat ggtcctgcct gaaacgccag tctgttgcgc cagctcacca
     1861 ggcgtgcgcg gctgttgctc cagcagatcg acaatcttta actggcgttc gtttagcatt
     1921 aatccacctg acggaactgg ctaaacgcgt tgactttctc cgcggttaag gtggtcagaa
     1981 aatcccgtat caacgtgagc agagcatcgt aatcggcttt tgaaatcata ccgctgttag
     2041 cgtgcagata gcgggttggc aggcacagcg caacaaccgg acgcccgccg cccatcacgt
     2101 tgtagcgccc gccatccgtc gcaccggttt tcatggtgga aaattgcagc ggtaaatcgt
     2161 tatgtgcggc acagcttttt aacgctgcta ccagtttctg gttggggaag tagcgcttgt
     2221 caaacagcat cagccccggc ccctggccca gtttcagcgg gtatttaatg ttatcaatgc
     2281 ccggaacatc gcctgcgaca gcggtatcca gcacgatcac tacatccggt ttaatgtgtt
     2341 ccgccgaggt ttgtgccccg cgtagcccaa cttcttcttc cacgctgccg acgccataca
     2401 gcgtaatttc gggattattc accgtctgca atagttcagc catcattgcg cagccgatgc
     2461 ggttatccag cgccttgccg accactttat cttcgcccca gcaggcaaaa ttggcttccg
     2521 ggctaataaa atcgcccatc gccacaccgc gtttttccac ctcatcgcga ctgtttgcgc
     2581 caatatcaat gaacatctca tcaaatgaca gcggctgttg cctttgcttt tccgttaacg
     2641 catgaggcgc gacggaacca atcacaccag ggattttcac tcccttgtgt gtgcgtatgg
     2701 ttacccggtg gttgagcatc gactgattcc accagccacc aatggtggtg aaacgcagaa
     2761 aaccgctctc gtcgatgtgg gtgaccataa agccgacttc atccatatgc ccgacaacgg
     2821 caacttttgg acctttattc cccttacggg caacaaagct gcccagacca tcaaaggtga
     2881 tctcattaac gcaaggttcc agcgtgttta tcagaatgtc gcgaacttcc tgttcatcgc
     2941 cgctgacggc gctggcttcg cacaactgtt gcagtaactc aatgttcatg gacaagctcc
     3001 tgttgcgcgg ctttcgcttt gcggcgtaac cacaatcctt tcaggacgat aatgaatgaa
     3061 atgttcagcg cgaggccaat cgccagcacc agataaaacg aacccaccgg cgacatcagg
     3121 ccgatcagcg ggtcaaagat acccaaacca ggtgccagac gtttgattcc gaaagcgatc
     3181 accagcacgc cggtaatgcc acccgccagc gtattggcgg taatcatcgg caacggagct
     3241 gccagagtgt aaggtatagc cggttcggtt gcgactgtgg caccgaccac aatggcacta
     3301 ctggcagcct ctttttcctg tcgggtaaac agtttgggcg caatgaacgt cgcaagaccc
     3361 gccgcaacgg gcggcatcaa cgcgacaacg ccgacgatgg cgtaccagtc ataaatgtgt
     3421 ttttccagca gtgagaagca ggcggtttta ttgatggggc cgcccatatc gaacgccagc
     3481 atcgcaccga ccagaaacgc tgctgcaaac ttcattgatg gaggaatggt attcaggaag
     3541 tggagcagcc cgcccatgag gtcggacatc accggcccga taacgtagta agtgagcacg
     3601 ccgaaaacca gcagagtaac gaacgggatc agcattgaac cgagcagcgg ttgtaatgct
     3661 ttgccaaggc gcactttgcg gaaccagaag acgaaatagc caatcgccag ccccagcacc
     3721 accgcgccga ggaatcccgc acccgactgg gtgccgagca gcgctttgtc gttcgccaga
     3781 tagcaaacca gaaacgccgg agcgaaagcc ggtttatcgg caatcgacga ggcaatataa
     3841 gcgcccatga tggggatcat aaagttgaag ccaaggtaac cgatggattc aactacccag
     3901 gtaaacgacg gcgcgccttt cgacatatcg gtatacggca aaccgaactg caccagcatg
     3961 ttggcaagcg ccaccaggat accaccgccg atgacaaacg gcagcgccgc agagacgccc
     4021 gccatcaggt ggctcatcac gctgccgctt tgcatttcct gtttgccaag cttcacgcca
     4081 ctatctgcgg caaaaagctg cgagtttgtc ggtaattcgc tgaaaatctg gtcgatattt
     4141 ttcaacgcct gggagatggc aatctcataa actttcttcc cggtaaaccg cgcgcgatca
     4201 tcaccgctca ggccacgccc ggtagcgaga atgacgtaat cggcagcggc aatctcttcg
     4261 ctggataagc ggttttcaac gccactggac ccttgagttt ccacttttat ggtatggccg
     4321 agagaacggg ctttctgttc cagggcttcc gccaccatgt aggtgtgagc gatcccggcg
     4381 gggcagttgg tgatcgcgac aatacgtaag gatgactcca tcgccacttc cttatgagct
     4441 gagggtttga tttaacaagg caagcacttg gtcggtatcg ccctgttgca gttgatgaat
     4501 aaattccttg tgaataattt tgcgacacag tgtgccgatg attttgacct gatcctcttc
     4561 gccgctttgc ggcacgccga ggcagatcca gcaattgacg tcttcgccat cgctggcttt
     4621 ccagtcaata gcctgcgctt tgcgggcgaa taatacgaac ggttgtttaa cgcaggcgct
     4681 tttaccgtgc ggcacggcga caccggaacc aaatccagta gagtgcattt tttcgcgcag
     4741 cagcagggtt tgcaggaact ggtgtgagtc ggtgataaaa ccgttttgta acgctattgt
     4801 cgccaactgc ttcagaacgg aataagcgcc attgccctga atattcaggt caatacagct
     4861 tgcagtaaga gctgccatta atcggactcc ataaaatata ttttaatcaa ttgtaatggt
     4921 ggagcgatta agtgatgaga ggcgatatgt cggtaaacaa tgacaatttg agacagagtg
     4981 aaaggtcaga tttgcggagt aatgcacata atggttattt aaataaacca catgaatcat
     5041 taatgggtta ttcattattt ttgtgattta ctcacaatgt atcagggaaa tataacttac
     5101 cgggagatgt aatatgttta atttttcaaa tcgaaattta aaatattgtg ccggaggcat
     5161 ctccggcaca ttgggcaatt acggcaggta aaacacttct tgc
//
LOCUS       DAJTMK010000230         5165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTMK010000230 DAJTMK010000000
VERSION     DAJTMK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.235"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            9..668
                     /locus_tag="OO135_003822"
     CDS             9..668
                     /locus_tag="OO135_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5399481.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            878..2938
                     /gene="ptrB"
                     /locus_tag="OO135_003823"
     CDS             878..2938
                     /gene="ptrB"
                     /locus_tag="OO135_003823"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5399482.1"
                     /translation="MLPKAACIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYAGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(2935..3597)
                     /gene="exoX"
                     /locus_tag="OO135_003824"
     CDS             complement(2935..3597)
                     /gene="exoX"
                     /locus_tag="OO135_003824"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5399483.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(3621..4277)
                     /locus_tag="OO135_003825"
     CDS             complement(3621..4277)
                     /locus_tag="OO135_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5399484.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(4379..4609)
                     /gene="holE"
                     /locus_tag="OO135_003826"
     CDS             complement(4379..4609)
                     /gene="holE"
                     /locus_tag="OO135_003826"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5399485.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            4748..5122
                     /gene="yobA"
                     /locus_tag="OO135_003827"
     CDS             4748..5122
                     /gene="yobA"
                     /locus_tag="OO135_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5399486.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
BASE COUNT         1331 a         1272 c         1308 g         1254 t
ORIGIN      
        1 gagcaaatat ggctaactgg ttaaatcaac tgcaatccct gcttgggcaa agcagttctt
       61 ctatctcctc gtctgcggat cagggattgg gcaaactgtt agtgccaggc gcattaggcg
      121 gactggctgg gctgctggtc gcaaataaat cagcacgtaa acttcttacg aaatatggca
      181 ccaacgcgtt actggttggc ggcggagcgg tagcgggtac ggtgctgtgg aataaataca
      241 aagataaaat tcgcgcggcg catcaggacg aaccgcagtt tggcgcgcaa agtacgccgc
      301 tggatgagcg tacagaacgt ttgatccttg cgctggtctt tgccgctaaa agtgatggtc
      361 atattgatgc caaagaacgt gcggcaatcg accagcaatt gcgtgaagcc ggcgtggaag
      421 agcaggggcg tgtactcatt gagcaggcaa tcgaacaacc gctggatcca caacgtctgg
      481 ctaccggggt ccgcaatgaa gaagaggcgc tggaaatcta tttcctgagt tgcgcggcta
      541 ttgatattga ccattttatg gaacgcagtt acctgaatgc actgggtgac gcgctgaaaa
      601 tcccccagga tgttcgtgac ggcattgaac gcgatctcga acagcaaaaa cgcactttgg
      661 cggaataatt ccgctctctg gatagcctga agttgactat ttcgggctat ccagaatcgc
      721 acagccattt gaacaggctg attttctctg tctgcataac attcgatatc cgcgccatta
      781 cctgtcatca tctaagcaat gactcccctg tttcgcttgc atccccggtg agttttgcca
      841 cccttaataa gatgtttcaa ccagaaagaa caataacatg ctaccaaaag ccgcctgcat
      901 tccccacgcc atgacgcttc atggcgatac gcgcatcgat aattactact ggctgcggga
      961 cgatacgcgt tctcagccag aagtcctgga ctacctgcaa caagaaaata gttacggtca
     1021 tcgggtgatg gcctcacaac aagccttgca ggatcgcatc ttaaaggaaa tcatcgaccg
     1081 cattccgcaa cgagaagttt ctgcgcccta catcaaaaat ggctaccgct atcggcatat
     1141 ttatgaacca ggctgtgaat atgctatcta ccagcgtcaa tcggcattca gtgaagagtg
     1201 ggatgagtgg gaaacattgc tcgatgccaa taagcgcgca gctcatagtg agttttattc
     1261 gatgggcgga atggcgatta cgcccgataa caccattatg gcgctggcag aagattttct
     1321 ttcccgacgc cagtacggca ttcgttttcg taatctggaa actggtaact ggtacccgga
     1381 actgctggat aacgttgaac ccagctttgt ctgggcaaat gactcctgga ctttctacta
     1441 tgttcgcaag catccggtga cgctgctgcc ttatcaggtc tggcgtcacg ccatcggtac
     1501 gccagcatcg caagataaac tgatctacga agaaaaagac gatacctatt acgtcagcct
     1561 gcataaaacg acctcgaagc actatgtagt cattcatttg gccagcgcca ccaccagtga
     1621 agttcgcctg ctggacgcgg aaatggccga tgccgagccg tttgtttttc tgccgcgccg
     1681 caaagatcac gaatacagcc ttgatcacta ccagcatcgg ttttatctgc gttccaaccg
     1741 ccacggcaaa aactttggct tataccgtac ccgcctgcgt gatgagcaac agtgggaaga
     1801 gttaattccg ccacgcgaaa acatcatgct ggaagggttt acgctgttta ccgactggct
     1861 ggtggttgaa gagcgtcagc gcgggttaac cagtttgcgc caaattaacc gcaagacccg
     1921 ggaagtcatt ggtattgcct ttgatgatcc agcctatgtg acctggattg cctacaatcc
     1981 agaacctgaa accgcgcgat tgcgttatgg ttattcttcc atgaccacac cagacacttt
     2041 gtttgaactg gatatggata ccggtgagcg tcgtgtatta aaacaaacgg aagttcctgg
     2101 ttttgatgcg gcgaattacc gcagtgaaca cctgtggata gtcgcccgtg atggcgtcga
     2161 agttccggtt tcgttggtct accatcgcaa acattttcgc aaaggacaca acccgttgct
     2221 ggtgtatggc tatggttctt acggcgcaag tattgatgcc gatttcagtt ttagccgctt
     2281 gagtttgtta gatcgtggct ttgtctacgc cattgtccat gttcgcggcg gtggtgagct
     2341 ggggcaacaa tggtacgaag acggaaaatt tctgaagaag aaaaatacgt ttaatgatta
     2401 tcttgatgcc tgcgatgcat tgttaaaact gggctatggc tctccttcgc tttgttatgc
     2461 gatgggcggg agtgcggggg gcatgttgat gggcgttgca attaatcaac gcccggaatt
     2521 attccacggc gttatcgccc aggtaccgtt tgttgatgtt gtaacaacga tgcttgatga
     2581 atcaattcct cttaccactg gtgagtttga agagtggggt aacccgcagg atccgcaata
     2641 ttacgagtac atgaaaagct acagcccgta tgacaacgtc accgcacagg cttatccgca
     2701 tttactggta acgaccggtt tacacgattc tcaggtgcaa tattgggaac cggcaaaatg
     2761 ggtcgctaaa ttgcgcgagc tgaaaaccga taaccatctt ttattgctct gtaccgacat
     2821 ggactcaggc catggcggta aatctggccg ctttaaatcg tacgcaggcg tagcgatgga
     2881 atatgctttt ctggtcgcgc tggcgcaggg aacattaccc gctacgcctg cggattaagt
     2941 attttccaga taatgtttca gtgttaaacg cagctccggg ctcatgctgt ccaggttatt
     3001 aaatgaccag cgcagatagc ccggatcgcg ttcggcaacg tcggaaaccg ctttgccacg
     3061 gtatttgcca aaggtgaagg tcgtcatcaa cgacggacgt ccggtgatat cggccatctg
     3121 ttctgccgtc cagccggagg tgttcataat atcgataagc aacgcggcgg tgatataaca
     3181 gtcatacaac gcgcggtgat gatgcaggcc cggcggcgtc tgtacattga gcttccgtgt
     3241 tttatataac gccatattgc tgtacttgat ccctggccac aaacgacggg ccagtttcat
     3301 agtgcaaatc cactcaccgg gcatctcagg cagtacgcgg cggtcaaagc tggcgttgtg
     3361 cgcgacatac cattcactac cgtaatagtg tgggatcaca tcttcaatcc acggtttatc
     3421 ggcgaccatg gcttcggtga tgcgatgaat cgccatcgct tgtggactaa taggacgatc
     3481 ggggcgcacc aggtggctca tggggttgac gatttttccg tcaatgacat caacagaggc
     3541 aatctcaacg atccctccct gcaaaccgca ggtttctgta tcgataatgc gcaacatggc
     3601 atgctccagg ccgaaaaagc ctagcgtaat ggaatgatat cgccttgcca accctgtgat
     3661 gaacgctgac cgactaacaa taatgaacca cgatcggcgc gaacgatgag acgaccatat
     3721 tcatcccata gtgcactgtt accacgggca tttgccatca gcacggcaat cgagtactga
     3781 tgtgaaaaaa attgtaagcg acgggccgac gccagcaggt cgggttctcc cagacattgc
     3841 cctgtagtaa acagtgaaca ggtcgggtcc atatccatac cttgcggttg ttcatcgacc
     3901 accgtgatgg ttctggaacg acggcccaga catgcaccat gactctgatg gtagatccca
     3961 ggcgtttttc gccacggtgc gaacaccgca attccacgaa taaagcgatc gttatattca
     4021 acgggaaggc cggcaatgat ggtcattcgc caggtcgttg cggcataaca aagcgggtct
     4081 aacagtgaga gatcgggtgg ggcaggaagg gcacgtcgcg aataatcaca ccccagtaaa
     4141 gaaagcgatg gaaaaactaa cagctgacat tgttgcctgg cggcggccct cacaaactcc
     4201 agatgatgag ctacctgttc ggtgagtgag gttttacgag gctcatattg cgctgctgca
     4261 actttccaga acgacataat gacttccttt tcaaaatgac tgcaaattat gggtgcggtt
     4321 tagaaaatac tcataattta gcaggcgtta tgtaagaaag tgtaactctg tataagtttt
     4381 atttaagttt gggctcgtaa ggtaaacgtg acagtttgac cgaagccaaa cggtgggcaa
     4441 taagcctctc gcgaaaccag ctgcgcaaat gttcaggctg ttcacgttca accgcttcag
     4501 cgatcaccgg catattgtag cgttctttaa atgccacccc ggccgccgcc aaatcgacat
     4561 tcactttatc catttctgtt tgatccagtt tagccagatt cttcagcatt cttaatctcc
     4621 tcatcattcg cgggcaaaag ttactaagag tgagccagga tggcaagccg gaattgatga
     4681 tcctcgactc agacattaga caaggttatg ctctgtaaaa aagttattac aaaaaggaat
     4741 agataatatg gcttcaactg cacgctccct tcgctacgcg ctggcaatcc tgaccacttc
     4801 gttggtgaca ccttccgtct gggcacatgc gcatttaacg catcagtatc ctgcggcaaa
     4861 cgcgcaagtg acagctgcac cgcaggcaat cactttaaac ttctcggaag gtgttgaaac
     4921 aggattcagt ggtgcaaaaa tcacggggcc aaaaaacgaa aatattaaaa cattaccggc
     4981 gaagcgaaat gagcaggacc aaaagcaact gattgttcca ctggccgatt cgctgaaacc
     5041 cggaacctac accgttgact ggcatgttgt ttcggtggac gggcataaaa cgaaaggaca
     5101 ctacaccttt agcgtgaaat aaatcatgct ggcgtttacc tggatcgcgc tgcgatttat
     5161 ccatt
//
LOCUS       DAJTMK010000231         5153 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTMK010000231 DAJTMK010000000
VERSION     DAJTMK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.236"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..114
                     /locus_tag="OO135_003828"
     CDS             <1..114
                     /locus_tag="OO135_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5399487.1"
                     /translation="KAESPLAVSLYDYINDASALACLINLSLNPSEVRGRK"
     gene            111..331
                     /locus_tag="OO135_003829"
                     /pseudo
     CDS             111..331
                     /locus_tag="OO135_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            328..1152
                     /locus_tag="OO135_003830"
     CDS             328..1152
                     /locus_tag="OO135_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399488.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPRGSVGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRGGL"
     gene            1149..1637
                     /locus_tag="OO135_003831"
     CDS             1149..1637
                     /locus_tag="OO135_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5399489.1"
                     /translation="MSLLNEVQKFIEAHPGCTSGDIADAFTGYSRQCVLQSASKLRQS
                     GRVAHRCEGDTRRHFPRLTERAQEAEPQPVRETRPVRNFYVGTNDPREILCLTRQAEE
                     LESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLRKSSKRAVSGDEWYLSGNYV
                     GA"
     gene            1637..2290
                     /locus_tag="OO135_003832"
     CDS             1637..2290
                     /locus_tag="OO135_003832"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR5399490.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYIIG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            2287..2676
                     /locus_tag="OO135_003833"
                     /pseudo
     CDS             2287..2676
                     /locus_tag="OO135_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            2696..3505
                     /locus_tag="OO135_003834"
     CDS             2696..3505
                     /locus_tag="OO135_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5399491.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     ISSQQTVELVHELTNTQNLGVDPVSMIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLFNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITCPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3513..4502
                     /locus_tag="OO135_003835"
     CDS             3513..4502
                     /locus_tag="OO135_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5399492.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPDVRAVFRNEEVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPTVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            4520..4900
                     /locus_tag="OO135_003836"
     CDS             4520..4900
                     /locus_tag="OO135_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HCR5399493.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
BASE COUNT         1265 a         1189 c         1485 g         1214 t
ORIGIN      
        1 aaagccgaat ctccgctggc tgttagcttg tatgactata ttaatgatgc ttcggcactg
       61 gcctgcctca taaatttgtc gctgaaccct tcagaggtaa gggggcgcaa atgatccgga
      121 atattttcaa acggtttacc aatcagactt tccgttgtcc tcgtccgggt cagtggtaca
      181 ccacgcctgc agggcatgtt ctacgtgtta gcctggtcgt gaatgtcaga aggtgatttg
      241 tgaaccgctg ggccgtaatt accgcatcag tatgccgctt atagcctttc gctccggaaa
      301 aaacatgaag catctcggag gtgctgcatg agtatggagc tgatggttaa agcgatgaaa
      361 attcgagtgg gaaatccatt gcgaaaactg gttctgatta agctggctga taatgccagc
      421 gatcagggtg agtgctggcc cagctaccag catattgctg accagtgcga gattagcaaa
      481 cgttctgtga tgaatcatat tgcggccctt tgtgagtccg ggctggtaaa aaaagtcacc
      541 cggaaaggtg aaaaaggtaa ctcaagtaat atctatctcc ttcatcttga tggtgcagga
      601 gattcactag ggggtagtgc aaataattca ctatctggtg cagcaaattc accaggtagt
      661 gcaggagttg caccaagggg tagtgtagga gattcaccca gaaccagtca ctcttttgaa
      721 ccagtcaaag aaccagtcaa tgaaccaata gctgttggtg catcagttga tgagtccgtg
      781 cgagttcgtt caaaccgacc ggaatactct ccggagtttg agcaggcatg gctggcatat
      841 cccaaacgtg ctggtggcaa ttcaaaatct gcagccttca aagcctggaa agcccgtttg
      901 aacgaggggg taaaccccga aaccatgctg gaaggtgtga aacgctacgc gggctgggta
      961 tctgcgatgg gcaatagcgg cacacaattt gtgaaacagg ctgtcacgtt ctttggtcca
     1021 gatcgtcatt tcgaagaatc ctgggaagtt cctgcggtat ctgcagccag acgcgaggac
     1081 ccgtacttca aagccagtta cgacaacgtg gactacagcc agatcccggc aggattcagg
     1141 gggggattat gagtcttttg aatgaagttc agaaattcat tgaagcccat ccggggtgta
     1201 cttccggaga cattgcggat gcttttacag gttactcacg gcagtgcgtt ctgcagtcag
     1261 caagcaaatt acgtcagagt ggtcgtgtgg ctcaccgttg tgaaggggat acacgcagac
     1321 atttcccgcg cctgactgag agagcgcagg aggcggaacc gcaaccagtt cgtgaaacca
     1381 gacctgtgcg caatttctat gtcggcacta acgacccgcg ggagattttg tgcctgaccc
     1441 gccaggcgga agaactggag tccaggggct tataccgtcg tgctgcaacg gtgtggatgg
     1501 cggcattccg tgaaagccac tcccagccag aacgaaacaa ttttctggcg cgtcgtgagc
     1561 agtgtttacg gaaaagcagt aagcgcgctg tatcgggtga tgagtggtat ctgtcaggga
     1621 attacgtggg ggcttaatga gtaataaata ttgccaggcg ctggtggaac tgcggaacaa
     1681 accagcccat gaactgaagg aagtgggcga tcagtggcgc acgccggaca acattttctg
     1741 gggaattaac accctgtttg gtccgtttgt tctggatctg ttcactgacg gtgataacgc
     1801 caaatgtgcc gcgtattaca cggcggaaga caacgcgctg gcgcatgact ggtcagaacg
     1861 tcttgcggag cttaaaggtg ctgcctttgg caatcccccg tacagccgcg ccagtcagca
     1921 tgaggggcaa tacatcatcg gcatgcgtta catcatgaag catgccagtg ccatgcgtga
     1981 taaaggcggg cgctatgttt tcctgatcaa agctgccacc agcgaagtgt ggtggccgga
     2041 agatgcagat catattgctt ttattcgcgg gcgtattggt tttgaactgc ctgcctggtt
     2101 tatcccgaaa gacgaaaagc aggtgccaac aggtgctttc ttcgctggtg ctattgctgt
     2161 ttttgacaag acctggaagg gaccggcaat cagctacatc gggcgcgatg aacttgaggc
     2221 atgtggtgag gcatttctgg cgcaggttcg ccagcaggcg gaaaaactgg tcagggagat
     2281 ggtggcatga agctaatact gccttttccg cccagcgtga acacgtactg gcgacacccc
     2341 aacaaagggg cgtttgctgg ttagagcctg ataagcgcgg cggggcgaaa attccagagc
     2401 gcggcgtgcg cagcaatagt tgagcagtta cgtcgtctgc cgaaaccaac gtcggcacct
     2461 gcttcagtgg agatcgtgtt gtttcctccg gataaccgga tccgcgatct ggacaactat
     2521 aacaaggcgc tgtttgacgc cctgacccac gcgggtgtgt gggaagacga cagtcaggtg
     2581 aaaagaatgc tggtggagtg gggaccggtt atcccgaaag ggaaggtcga gatcactatc
     2641 agtaagtacg agaaaacggc gggtgcagcc gcctgatcaa gaggagaaac gaagtatgaa
     2701 taatctgatg gtcattgatg gtattgaagt tcgtcgtgat gcttatgggc gttacagcct
     2761 gaacgatcta catcgcgcag cagtagcatc tggtgcaaat gccagaacca aggagccggg
     2821 aaagtttatt tccagccaac aaactgttga gcttgttcat gaattgacca acacccagaa
     2881 tttgggtgtt gacccggtga gtatgattca tgggggaaat gaacggggaa cgtatgtctg
     2941 caaggaactg gtgtatgcct atgcaatgtg gatcagcccg tcattccatc tgaaggtgat
     3001 ccgtactttc gacatggtaa ccagcgcacc ggaaaaatta tccggacagg ctgctgacaa
     3061 gatgcaggct ggtgtgattc tgctggactt tatgcgccgg gaattaaacc tgtttaactc
     3121 ttcagtgctt ggtgcctgtc agaaactcca ggaggctgtt ggcttaccga atctggcacc
     3181 gcgctatgcc attgatgctc ctgctgacgc gcctgatggc tcaagtcgcc ccacgctgtc
     3241 gctgagtgca ctgctgaaac agtatggtat ccgccttacg gctaatcagg catatcacca
     3301 gatggtgaag ctggggatcg tcgagcagcg cgaacgatac agccgtaccg cgattaacaa
     3361 catcaaaaaa ttctggtcgc tgacagcgaa aggctgcatg ttcggcaaga acatcacctg
     3421 tcctgcaaat ccgcgcgaga cgcagccgca tttcttcgaa tcccgattcc ctgagctgtt
     3481 aaagctgctc gataccgttc attgaggtga ccgtgagagc actactgacc cctgaaattg
     3541 ccccgcgtat ggggatcgta ttgttcagac caggttcaga gctgatgccc ctgtttatgc
     3601 aggggcgtgt cctgctggag cctgagccgg agcgttattc atctttcgcc agtggtgccg
     3661 ttccggcggc atcacaaccg ctggcggatg atcctgacgt tcgggccgtg ttccgcaatg
     3721 aggaagtgat ccgtcgtgct ggtggcgtgg aatgtcttga aagctggtta cttcgtgaaa
     3781 aaggctgcca gtggcctcat tccgactggc acagcgagaa catgaccaca atgcgacacg
     3841 ctccgggtgc aatccgtctg tgctggcact gcgataatca gctgcgcgat cagttcacgg
     3901 aacggctgga atcaatggca acggataact gtgcccgctg ggtgttgtct gttgtgcgtc
     3961 gggatctcgg ttttgatgac agtcacgttg tgacaatgcc ggaactgtgc tggtggctga
     4021 ttcgtaatga tctggcggat gccttaccgg aaagtgcagc ccgtaaggca ctgagattac
     4081 cgaagcctgt tgtgccgact gttacccggg aaagtgacct tgtgccttcg gttcctgcca
     4141 ccagcatcat ccaggataag gcgaaaaagg tgctggcgct gaaagtggat ccggagtcgc
     4201 cggagtcttt tatgttacgc ccaaaacgtc gccgctgggt taatgaaaag tacacgcgct
     4261 gggttaagac acagccgtgt gcatgttgtg gaaagcccgc tgatgatccc caccacctga
     4321 taggtcacgg tcagggggga atgggtacaa aagcgcatga cctttttgtg ttgcctttgt
     4381 gcagaaaaca ccatgacgaa ctgcatgcgg ataccgtggc atttgaagag aagtatggct
     4441 cccaactgga gctgatattt cgttttatcg atcgcgcgct ggcgattggt gtgctgtcct
     4501 gattttgtgg agaaagttga tgcgtgatat tcagatggtt cttgaacgct ggggggcatg
     4561 ggctgcaagt ggtaacaccg gggtggacta ttctccgatc gctgccggat tcaaaggact
     4621 tttaccatct gccactaaac cacgtccggc ctgctgcgat gatgacggac ttatcattga
     4681 aaactgtctt gctcgtctga agcagaaaaa acctgaggag tattcgcttc tcattgctca
     4741 ttatttgttg cgaatatcaa aaagacagat agccaggacg agaaagaaaa gcgaaaaagc
     4801 aatacgaatt gagatgcaga tagccgaagg gtttattgac ggatgtttgt ctgtgctggg
     4861 ggtaagactg gagatggacg actggctgct aaaaaagtaa aaaatgatta gtgcggtccg
     4921 caaaaagtat gtcagtatgt taagattggt cactccgtca tacagcttaa acctgccgcc
     4981 tggcgggttt tttatgactg aaatcgcgtc agtacagtaa acgcgctggt ggcggtgaat
     5041 acctgtcttt cagcttgctg gctttttcga caagagttat tggtgtgtca cgttaaccgg
     5101 aaaagggaaa aagacatgct gaaacagcag gatatgacag aaaccgccag agt
//
LOCUS       DAJTMK010000232         5080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTMK010000232 DAJTMK010000000
VERSION     DAJTMK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.237"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            29..1915
                     /locus_tag="OO135_003837"
     CDS             29..1915
                     /locus_tag="OO135_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5399494.1"
                     /translation="MATGLLSLIIPVPLTTVQWANKHYYLPKESSYTPGRWETLPFQV
                     GIMNCMGNDLIRTVNLIKSARVGYTKMLLGVEAYFIEHKSRNSLLFQPTDSAAEDFMK
                     SHVEPTIRDVPALLELAPWFGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSVDV
                     VCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGSCQIEKAANESAH
                     FMRFYVPCPHCGEEQYLKFGDDASPFGLKWEKNKPESVFYLCEHHGCVIHQSELDQSN
                     GRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALK
                     DPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTTAVPARVVYLTAGIDSQRNR
                     FEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRVCW
                     DIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAK
                     EILYARMKADPTPVDEATSYAIRFPDDPEIFSQTEAQQLVAEELVEKWEKGKMRLLWD
                     NKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRPTLKELAAKLSGGVN
                     GYSR"
     gene            1899..2105
                     /locus_tag="OO135_003838"
     CDS             1899..2105
                     /locus_tag="OO135_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399495.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2102..3694
                     /locus_tag="OO135_003839"
     CDS             2102..3694
                     /locus_tag="OO135_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5399496.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYTGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESDLDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            3684..>5080
                     /locus_tag="OO135_003840"
     CDS             3684..>5080
                     /locus_tag="OO135_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5399497.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGILLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAVQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAL"
BASE COUNT         1132 a         1224 c         1613 g         1111 t
ORIGIN      
        1 gcagaaggca gctaatgcag ccggtgcgat agctacaggg cttttatctc tcattattcc
       61 tgttccactg acgacagttc agtgggccaa taaacattat taccttccta aagagtcgtc
      121 ttataccccg gggcggtggg aaacactgcc gtttcaggtt ggcatcatga actgtatggg
      181 caacgatctg attcgtacgg ttaacctgat taaatctgcc cgtgttggtt atacaaagat
      241 gttgctggga gtggaggctt attttattga gcataaatca cgcaacagcc ttctttttca
      301 gcccacggac tcagctgctg aagattttat gaaatctcat gttgagccaa cgataaggga
      361 tgttcctgca ttgctggagc tggctccatg gttcggaaga aaacaccgcg ataatacgct
      421 caccctgaag cgtttttcct ccggtgtggg gttctggtgt ctgggtggtg cggcagcaaa
      481 aaactaccgt gaaaaatccg tggatgtggt ctgttatgac gagctttcct cgttcgaacc
      541 ggatgttgaa aaagagggtt cgccaaccct gctgggggat aaacgtattg agggctctgt
      601 atggccaaaa tccattcgcg gctcgacgcc taaaatcaaa ggctcctgcc agatcgaaaa
      661 agccgctaac gagtcggcac acttcatgcg tttttatgtg ccctgtccgc actgtgggga
      721 ggagcagtat ctgaaatttg gcgatgatgc ctcgcctttc ggtcttaagt gggagaagaa
      781 taagccagaa agtgttttct acctttgtga gcatcatggc tgtgtgatcc atcagtctga
      841 gcttgaccag agtaacgggc ggtggatctg tgaaaacacg ggcatgtgga cccgtgacgg
      901 cctgatgttt ttcagcgccc ggggtgatga aattccgccg ccgcgctcca tcactttcca
      961 tatctggacg gcgtacagtc cgttcaccac ctgggtacag attgtctatg actggctgga
     1021 tgcactgaaa gatcccaacg gcctgaaaac ctttgtgaac accacgctgg gcgagacctg
     1081 ggaagaggcc gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcgtta
     1141 cacgacggcg gtgcctgccc gggtggttta tctgacggcg ggcattgact cgcagcgaaa
     1201 ccgttttgag atgtatgtct ggggatgggc tccgggagag gaagcctttc tggtggataa
     1261 aatcatcatt atggggcgtc ccgatgagga agagacgcta ttacgtgtgg atgcggcgat
     1321 caacaaaaaa taccgccatg cagacggaac cgaaatgact atttcccgtg tctgctggga
     1381 catcgggggg attgatggcg aaatcgttta tcagaggtca aaaaaacacg gtgttttccg
     1441 ggtgctgccg gtaaaaggcg catctgtcta tggcaagccg gtgatcacca tgccaaaaac
     1501 ccgcaatcag cggggcgtgt atctgtgtga agtggggacg gacaccgcaa aagaaattct
     1561 ctatgcccgt atgaaagccg atcccacgcc tgtggatgaa gccacgtcgt atgccatccg
     1621 ttttcctgat gatccggaga ttttttcgca gacagaggcg cagcaactgg tggcggaaga
     1681 gcttgtggag aagtgggaaa aaggaaagat gcgtctgctg tgggataaca aaaagcggcg
     1741 taacgaagcg ctggactgcc tggtgtatgc ctacgcggca ttacgtgtgt ccgtgcaacg
     1801 ctggcagctt gatctggctg tactggcaaa atcccgggaa gaagagacga cccggccaac
     1861 ccttaaagaa ctggcagcga agctgtccgg aggagtgaat ggttacagtc gctgaactgc
     1921 aggcgctgcg tcaggcgcgc cttgatttat taaccggtaa acgggtggtg tctgtccaga
     1981 aagatggtcg cagaattgaa tatacggcag cttctctgga tgagcttaac cgggcgatca
     2041 atgatgcgga gtcggtactg gggacaaccc gccgtcgccg tcgtccgctg ggagtgaggt
     2101 tatgaaacga acgcctgtcc tgattgatgt gaacggcgtt ccgcttcggg agagcctcag
     2161 ctacaccggt ggcggtgcag gatttggcgg gcaaatggca gagtggttgc caccctcgca
     2221 gagtgccgat gcggccctgc tgcccgcgtt gcgtctgggg aatgcccgtg cagatgatct
     2281 ggtgcgcaat aacggaatag cggccaatgc ggtggcactg cataaggatc acattgtcgg
     2341 gcatatgttt ctgattagct accgtccgaa ctggcgctgg ctggggatgc gggagaccgc
     2401 ggcaaaaagt tttgtcgatg aggtggaggc ggcctggtca gaatacgcag aagggatgtt
     2461 tggtgagatc gacgtggaag ggaaacgcac gtttacggaa tttatccgtg aaggtgtggg
     2521 cgttcatgcg tttaacggcg aaatctttgt gcagccggtc tgggatacgg agagtacgca
     2581 actgtttcgt acgcgtttta aagccgtgag tccgaaacgg gtggacacgc caggacacgg
     2641 tatggggaac cgttttctgc gggctggtgt ggaggttgat cgatatggcc gtgccgttgc
     2701 gtaccatatc tgtgaggatg attttcctcg ctccgggagt ggacgatggg aacggatccc
     2761 gcgtgaacta cccaccgggc gtccggccat gctgcatatt ttcgagccgg tggaggacgg
     2821 gcagacccgt ggagccaatc agttttacag cgttatggaa cggctgaaga tgctggattc
     2881 cctgcaggca acacagcttc agtcggccat agtgaaggcg atgtatgcag cgacgattga
     2941 aagtgacctt gataccgaaa aggcctttga atatatcgcc ggtgcgccgc aggggcagaa
     3001 ggataatccg cttattaata ttctggataa gttctccacc tggtatgaca cgaatagcgt
     3061 gacgctgggc ggtgtcaaaa ttccgcacct tttccccggt gatgatctga aacttcagac
     3121 cgcgcaggat tcagacaatg gattttcggc gcttgaacag gcgctgctgc ggtatatcgc
     3181 cgccggtctt ggcgtttcct acgaacagtt gtcccgtgat tactcgaagg tcagttattc
     3241 aagtgcccgc gcatccgcca atgagtcgtg gcgctatttt atggggcggc gaaaatttat
     3301 tgcgtcccgg ctggccacgc agatgttttc ctgctggctg gaagaggcac ttcttcgggg
     3361 gattattcgt ccgccacggg cacgttttga tttttatcag gcgcgatcag cctggtcacg
     3421 ggctgagtgg attggagccg gaagaatggc cattgacggg ctcaaggagg ttcaggaatc
     3481 agtgatgcgc attgaggccg gactgagcac gtatgagaaa gagctggcgc tgatgggcga
     3541 ggattatcag gacattttcc gccagcaggt cagggaatct gcagagcggg aaaaagccgg
     3601 actctcacgt ccggtgtgga tagcgcaggc gtatcagcag cagatagcgg agagtcgcag
     3661 gccggaagag gagacaacac cacgtgagac gtaatctttc acacattatt gccgcagcat
     3721 tcaatgaacc gctgcttctg gagcccgcct atgcgcgggt tttcttttgc gcgctcgggc
     3781 gcgagatggg cgcagcaagt ctttcggtac cacaacagca ggtacagctt gatgctcccg
     3841 gaatgctggc tgaaacggac gagtacatgg ccggaggtaa acgaccggcc cgtgtttacc
     3901 gggtggtgaa cggtattgcg gtactgccgg tgaccggcac gctggtgcac cggctggggg
     3961 ggatgcggcc attttccgga atgactggct atgacggcat tgtcgcctgt cttcagcagg
     4021 caatggcaga tagccaggtg cggggcatac tgctggacat tgacagtccg ggtgggcagg
     4081 ccgccggcgc gtttgactgc gctgacatga tttaccgcct ccgtcagcag aagccggtct
     4141 gggcactgtg caatgacacg gcctgttctg cagccatgct gctggcgtcg gcctgctccc
     4201 gacggctggt tacccagaca tcccgtatcg gctccattgg cgtgatgatg agccatgtca
     4261 gctatgccgg tcatctggcg caggccggtg tggatatcac gctgatttat gccggggcgc
     4321 acaaggtgga tggcaatcag tttgaagcgt tgccggcaga ggttcgccag gatatgcagc
     4381 agcggattga tgcggcgcac cggatgtttg ccgaaaaagt ggcgatgtat accgggttgt
     4441 ctgtggatgc ggtcacggga acagaggccg ccgtttttga aggtcagtcc ggcattgagg
     4501 ccgggctggc ggatgaatta atcaatgcgt cggatgccat cagtgtgatg gccacggcgc
     4561 tgaacagtaa tgtcagagga ggcactatgc cgcaattaac tgcaacggaa gccgccgtgc
     4621 aggagaacca gcgagtgatg gggatcctga catgccagga agcgaaagga cgtgaacagc
     4681 ttgccacgat gctggcaggg caacagggca tgagcgttga acaggcccgg gcgattctgg
     4741 ccgcggcggc accgcagcag ccggtggcat ccgcgcagag tgaagccgat cgcattatgg
     4801 cgtgtgaaga agcgaacggt cgtgaacaac tggcagcaac gctggcggcg atgccggaga
     4861 tgacggtgga aaaagcccgc ccgatcctgg ctgcttcacc gcaggcgaat gccggaccat
     4921 cactccgtga tcagatcatg gcactggatg aggcaaaagg ggctgaggcg caggctgaac
     4981 agctggctgc ctgcccggga atgaccgtgg agagcgcccg ggctgtgctg gctgcgggat
     5041 caggtaaggc agaaccggtc tctgcatcca caaccgccct
//
LOCUS       DAJTMK010000233         5080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTMK010000233 DAJTMK010000000
VERSION     DAJTMK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.238"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..701)
                     /locus_tag="OO135_003841"
     CDS             complement(<1..701)
                     /locus_tag="OO135_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5399498.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPYVAISLDR
                     TEGAKLEFYEAEAKKQAKQFFMDVAAGLCEGNEPLQEKRPIILEAQDVLITYRGKLPG
                     IITCSLKMPPSTLRSEKEASCLQSEYSITVKSAGEEGNKRYFIASAPDKDQEWECNRP
                     SFVVYGDGGKITISENGKLTPPSHQHSEALIEFAIDYLKNNKKQGLMKCIGRCMGYLQ
                     IAAEIEALASGADKDA"
     gene            complement(706..921)
                     /locus_tag="OO135_003842"
     CDS             complement(706..921)
                     /locus_tag="OO135_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5399499.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAVRGE"
     gene            complement(1072..2967)
                     /locus_tag="OO135_003843"
                     /pseudo
     CDS             complement(1072..2967)
                     /locus_tag="OO135_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            complement(3470..3546)
                     /locus_tag="OO135_003844"
     tRNA            complement(3470..3546)
                     /locus_tag="OO135_003844"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3510..3512),aa:Arg,seq:tct)
     gene            complement(3562..3637)
                     /locus_tag="OO135_003845"
     tRNA            complement(3562..3637)
                     /locus_tag="OO135_003845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3600..3602),aa:Arg,seq:tcg)
     gene            complement(3644..3719)
                     /locus_tag="OO135_003846"
     tRNA            complement(3644..3719)
                     /locus_tag="OO135_003846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3684..3686),aa:Ile,seq:cat)
     gene            complement(3769..4818)
                     /locus_tag="OO135_003847"
     CDS             complement(3769..4818)
                     /locus_tag="OO135_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR5399500.1"
                     /translation="MANTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYEAKGRVLKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKSHYQLVSTYSELSRQYVELQSEYKNLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKTAEMLQQIINASSRPGDQVADFFMGSGSTVKAALALGRRAIGVELET
                     GRFEQTVREVQDLIV"
BASE COUNT         1180 a         1447 c         1190 g         1263 t
ORIGIN      
        1 gcatccttat cagcaccact ggccagcgct tcaatctcag cagctatctg cagatatccc
       61 atgcaacgac caatgcactt catcagcccc tgctttttat tgttcttcag gtaatcaatg
      121 gcaaattcaa tgagcgcctc actatgctgg tgcgatggcg gtgttaattt cccattttct
      181 gagatggtta ttttcccgcc atctccgtat acaacaaagg atggccggtt acactcccat
      241 tcctgatctt tatcaggtgc agacgcaata aaataacgtt tatttccttc ctctccggca
      301 cttttaaccg taatggagta ctcagactgc agacaagacg cctctttttc tgaccgcagt
      361 gttgacggcg gcatcttcag agaacaagta attattcccg gtagttttcc tctgtaggtt
      421 atcaacacat cctgcgcctc taaaatgatg gggcgctttt cctgcaacgg ttcgttccct
      481 tcacataacc cggcagcaac atccatgaaa aactgcttcg cctgcttttt cgcctccgct
      541 tcgtaaaact ccagcttggc accttcagta cggtcaagac taatcgccac atatggcaac
      601 aacagtgacg gatacccacc aatttccagt gccacagtaa cagtaatctt atccgggtaa
      661 ttatttatcc ctttaacaac cagttcgtat tttttcttca tcactttact ctccccgtac
      721 agccttacga cggtcctctc tgattttgaa atacaggtta gtcagatatg tcagcagccc
      781 aaacagcaga ctccccagca cgcctattgc cgcccactga gacggggaaa ccctgtccag
      841 caactgcagg aaccagtagc ccgttcccac cgctgacgtg gtgtatgaca cacctgttgt
      901 gattttttcc atctggtaca taccccgtct cccgttatcc ggaagctgac aacaataaaa
      961 aaagccacca gttaagtact gatggctctg ataactcatg caggcatctc agacgaccca
     1021 ctgacactac cggtgagttt aacgatacct tccatttgac tggctcactt tttatgatga
     1081 tgccggtgca tttatctcca gcaccagact ttctatctca acgccatacg ctgcattttt
     1141 ggtaatatcc gtcagcgtca gtgcatttag tcccactgcc agactgtctt ttatggcctg
     1201 gaatgccggg ccagccactc cattcagttt cggagtaacc gtggcactgc cggcggtgaa
     1261 caccagctcc agcgtctgcc agtcgttact gtaattcccg aactcgccca actttgtgtt
     1321 tccggctttc ttgtgatgca tcagattccg tttgccgtct gtggtctggg tgaagaacga
     1381 catcaggaac gggttaccag tcccggtcat cgccacgacg tcaggtaacg ctacatcggt
     1441 atacagataa attcccagac cgaactgatt gttggtcagt gcgcctgaca gtcgaaactt
     1501 acagctcagt ctgccacccc gtgtcagcag ggagactgcg tcatccaccg ggcgcgtcag
     1561 ggaccaggct ttattgctct gcttggcgat cttaaataca ccacccgaca actgaattcc
     1621 gccgtcctta atggtccagc cctgcgcagc agcctctccg gctgtcggca gcagggagat
     1681 tgtgcgaacg gacgtatctg cagacggacc cgatggcgtg tcgccgccgg gcgagggttt
     1741 gatttccggt gccttaccac tgatgaaggc tgaggtgcgc ccggctgcgt tcagaatagc
     1801 ggttgccata cgatcgggaa taatgccacg acgcgcccat gagctgaaat ttgtcgggcg
     1861 atttgatgat acccagtttt tgttcgttcg ggatgccgaa ccgtaataac cagacccgac
     1921 aatatcagga tcttctgacg ggttgtttgt cggtgtatta actccgctac catcggtcat
     1981 aaagggaaca aaataaatct gctgggattc tttaccttta tatgcaccat ataccacttc
     2041 atattgcgta ccgtgttctt gtttccacgc gtatgtcgtg tcgccacaaa tccaggggac
     2101 tgttgccgga cttccaccgt gacactgcgc cgccagcccg gcaaggtcag cacggaactg
     2161 ctgtaccatt gcaagaaatg ctgctggctg ctgggcgtaa ctggcattcg tcatatcgaa
     2221 ttccccctgc atccagcata tcgccagcaa aacgtttttc gggtttttct gcaatgctgc
     2281 cttcgtgcgg aaaagcagat cctgatataa cggcttaccc actccccagc gagccgaatc
     2341 ctgactggcc cccgtggact cgctgaatgt cccctccgtg ccctgggtga atgccgaacc
     2401 accacgacag catggtacca gcaggatccc cgcgttatta gggatatacg gaagcagttt
     2461 tttggcaata tgtaagccct gtccgacaca gccgtactgc cctttgctca ggtcagcccg
     2521 gggatggtta atcgtactca tatcctgaac atcatgcaga caatggtcag caggaatgat
     2581 gtcgttaaat acgcatactt caccaccggg agtcactgtg ttacgacggg ccagttgctt
     2641 aatgcgcgga tggggcgcat cgtatgaatc cggaagcgga agcccttcac cgtaagccat
     2701 ggcattggac tgcccggcca gggcaacaac aaattaatac tccgggttgc tggtggtgct
     2761 gataactgcg ccttctccat ccgacggctt caccaccaca ggtgtggtga catcaccttc
     2821 cgccgcaatg gcctgcatca gggtataagg cgtgatggcc accggactgc caaatggctg
     2881 ccacccctcc ttcagttttt gtgtcagtcg ctccgcaagg tctgacggcg acgccgccct
     2941 gaccacgtca tagtgtttaa atgccataaa tcctcccggc ctggataata ttgtgagtaa
     3001 aatgaggagc gggctgaagt ccggaagtta caggacaatg gcagaagata gacaacagcc
     3061 cgcaatatga aaaaggccgc gctattgcgc agagtgatta ctgtcggata ttattcacca
     3121 gctgaaatat tacttcacgt tttgttgttt attccttgcc gcccgcgtct cccagcgcgg
     3181 gctttttttg tccataagaa agcccctccg gagaggggct ggagagtggc gctatgtgcc
     3241 attgcatggt gccgggtgcc tcccggtgag ttcagtatca gcacctgaac ccgcacagaa
     3301 aggataaggg tcggtgacaa aacaccagtt gctgattgcc cctccgcaca gggggattca
     3361 ccatgccagt ttcttttaac aaactccccg caaaccagac aacagtcaac cgcctgaatt
     3421 gtgagacatt taaaaaaagc ccgcaaaagc gagccaggga aaataagtgt ggcgcgttct
     3481 actgtattcg aaccagtgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa
     3541 caacgcagga tacagataat gaaccgcctt cggggacccg aactccgcgc aaccagcttc
     3601 gaaggctggc gctctttcct gatgagctaa tggcggtatg tgatggtggc ccttgctgga
     3661 tttgaaccag cgacctggcg attatgagtc gctcgctctc accactgagc taaagggccg
     3721 ggagcagaat aataatggtg cgtaattaat tctgcaatct catccgtttc aaacgattaa
     3781 atcctgaact tccctgactg tctgctcaaa acgtccggtc tccagttcaa cgccaatcgc
     3841 acgacgcccg agcgccagtg ccgcttttac cgttgaacct gagcccataa aaaaatctgc
     3901 aacctggtct cccggacgac tgctcgcgtt gattatctgc tgcaacattt ctgccgtttt
     3961 ttcgcacgga tgtttccctg gatagtactg caccggttta tacgtccaca catcggtgta
     4021 cggcacctgc accgtcacac cgaaatacct ccgcaaattt ttatattcac tctgcagctc
     4081 cacatactgc cggctcagct cactgtatgt gctgaccagt tggtaatggg acttttccag
     4141 ttctccccgc tgatgtttct cttctgccac ccgggcaaac agcgactgta atttcagata
     4201 atcgctttcg ttcggtagct gccactgact ggcactgaac cagtgcgaca ccatgttttt
     4261 ctttcctgtg gcatctgcaa tctgttttgc cgttatcccc agggcagctc gcgcatcacg
     4321 aaagtaagca atcagcgggg ccatcacatg ctgtttcagt accctgccct tcgcctcata
     4381 gccatcatct ttcgggcggt acggcccctg ataatgttcc gcgaacagaa tgcgctctgt
     4441 ggcggggaaa tacgcccgca ggctttcctt gttgcacccg ttccagcgtc cggacggctt
     4501 tgcccagata atatggttca gcacactgaa gcgttcacgc atcatgattt cgatatcaga
     4561 tgccaggcga tgaccacaga acaggtaaag acttccggca ggtttcagca cccgccagaa
     4621 ctgcgccaga cactggtcca gccacttcag gtaatcatcg tcgcccttcc actggttatc
     4681 ccagccctca ggcttcactt taaagtacgg cgggtccgtg actatcaggt caaccgaatt
     4741 ttcgggtaac gaccggataa attccaggca gtcggcgttg attaactcac aactggatat
     4801 ttttacagta ttagccatag atcaataagc acttctctga taggctcata ccgcttttgc
     4861 gcaaagcaga tgggcctgag gtttgcttgt gaccccaacg catgagcaga tggctggtgg
     4921 gtgcccctaa cacccaccag ccgcccattt accacaaata aaaaagcctt cactgcggaa
     4981 ggcgtctgta acaaccgaac tgatagtctg ccagacccgc cataaccagc tgggtcagta
     5041 ttaactggca gcgttcgcgt gaaaggtaag tattctgcgc
//
LOCUS       DAJTMK010000234         5057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTMK010000234 DAJTMK010000000
VERSION     DAJTMK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.239"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1314)
                     /locus_tag="OO135_003848"
     CDS             complement(<1..1314)
                     /locus_tag="OO135_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5399501.1"
                     /translation="MRHFIYQDEKSHKFWAIEQQGNELHINWGKVGTNGQSQIKSFAD
                     AAAAAKAELKLIAEKTKKGYVENASANVHIPPITKATPEVETSPESKNQRPWLADDAV
                     IRLTDDINRFAFPHRSRPREINYLHKDGQIWQLIANNTRAYDPANNYRSYPENWQQAF
                     AELQMRVQGNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIALLALQL
                     RYKPVKGAVTTIIPPNHKAESLPSWHQRLCHHLSLASEDEWQRCVDKVLAAIPTLSPA
                     RQPFAALLLPECPDIANAMALRYADQNVPAMTWLSMMVSDDVALAIQEKYGFPPLYND
                     FRKYLATLLANNGMRGVSRILLKLPVDYPVKYTDLFTHIHANAEDLVKWLWKTNHPDA
                     IQILILGVNGKKKHLEYLSKACQKHPAAAIAAYATLLAIHENNEWR"
     gene            complement(1455..3488)
                     /gene="metG"
                     /locus_tag="OO135_003849"
     CDS             complement(1455..3488)
                     /gene="metG"
                     /locus_tag="OO135_003849"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5399502.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWIHSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYRTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3620..4729
                     /gene="apbC"
                     /locus_tag="OO135_003850"
     CDS             3620..4729
                     /gene="apbC"
                     /locus_tag="OO135_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5399503.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT         1226 a         1236 c         1288 g         1307 t
ORIGIN      
        1 acgccactca ttattttcat gtattgccag caaagtagca taagcggcaa tagccgcagc
       61 gggatgtttt tggcaggctt tgcttaagta ttccaggtgc tttttcttgc catttacacc
      121 gaggatcaga atttgaatcg catccgggtg attcgttttc catagccatt tgacaagatc
      181 ttcagcattg gcgtgtatgt gagtgaaaag gtccgtgtat tttacgggat agtcgacagg
      241 tagcttgaga agaatccggc ttacaccacg cattccatta tttgccagca acgtagccag
      301 atatttgcgg aaatcattat atagcggtgg gaaaccatat ttttcctgga tggccagggc
      361 aacatcatcg cttaccatca tacttaacca ggtcatcgcc ggaacgtttt ggtctgcata
      421 acgtagcgcc atagcattgg caatatccgg gcattcgggg agcagtaacg ccgcaaaagg
      481 ttgccgtgct ggggatagcg tcggaatggc ggcgagtact ttatctacac agcgttgcca
      541 ctcatcttct gaagcgagtg aaagatgatg acatagacgt tgatgccaac tcggcagcga
      601 ttctgctttg tgattaggtg gaatgatggt ggttactgcg ccttttactg gtttatatct
      661 aagttgaaga gcgagaaggg caatttcaac ggcactttcc agaccacagc gaatgactaa
      721 gtcatcgacc agttcatctg cagagtaaga gttccagaaa ctccagagca atgctgcatc
      781 agattgtgca ctccctgtct gttgattacc ctgaactcgc atttgtaact cagcaaaagc
      841 ctgctgccag ttttctggat aagaacggta attgttggcg ggatcatatg ccctggtgtt
      901 attcgcgatg agctgccata tttgaccatc tttgtgcaga taattgatct ctctggggcg
      961 agagcgatga ggaaaagcaa atcgattaat atcgtcagtt agccgaatga ccgcatcatc
     1021 agccagccag gggcgttggt ttttactctc aggggaagtt tcaacttcag gagtggcttt
     1081 ggtaatgggg gggatatgca cgtttgctga agcattttcc acatagcctt ttttcgtttt
     1141 ctctgcgatc agcttaagct ccgcttttgc cgctgccgca gcatccgcaa aactttttat
     1201 ctggctttgc ccgttagtgc cgactttgcc ccagttgata tgcagctcgt ttccctgctg
     1261 ctcaatcgcc cagaatttat gtgatttttc gtcctgatag ataaagtgtc tcattctgca
     1321 tccctgtaat tttatccagt tgaatgcaga tgctaccagt atttatgcgg gttagagaga
     1381 gacaaaatcg taagaaaaca actttaggaa tttataaaga aaaggcgctg tcgatgcagc
     1441 gccttgaagg gggattattt cacctgatga cccggtttag caccagcatc cgggcttagc
     1501 aggaaaatat ctttcccgcc aggaccggca gccatcacca tgccttcgga gataccgaag
     1561 cgcattttac gtggtgccag gttagccacc ataatggtgt gacgaccaat cagtgcctgc
     1621 ggatccgggt aagcagaacg aatgccggag aagacattac gcttttcgcc gccgagatcc
     1681 agcgtcaggc gtagcagttt gtcagaacct tcaacaaact ctgcgttttc aatcagcgcc
     1741 acgcgcaggt caactttggc gaagtcgtca aaggtgatgg tttcctgaat cggatcatct
     1801 gccagcgggc cagttaccgg cgcggcagct gctttcactt cttctttaga cgcttccacc
     1861 agtgcttcaa cctgcttcat atctatgcgg ttatacagcg ccttgaacgg attcacttta
     1921 tggcccagca gcggttgctg gataccatcc caggtcagtt ccgtattaag gaatgcttct
     1981 gcgcgctcgg tcagtttcgg cagtaccggc ttcaggtaag tcatcagcac gcggaacagg
     2041 tttatgccca ttgagcaaat tgcctgcagg tcggcatcgc ggccttcctg tttcgctacc
     2101 acccacggag cctgttcatc gacatagcgg ttagccagat cagccagcgc catgatttcg
     2161 cgcactgctt taccaaattc gcggctttcc cacgcttcac caatcacttc agcggcatca
     2221 gtgaaggttc tgtacaactg cgggtcagcc agttcgcttg ccagcacgcc gtcaaaacgc
     2281 ttgttgataa agcccgcgtt gcgggaggcc aggttaacca ctttgttcac gatatcggca
     2341 ttcacgcgct gaacgaaatc ttccaggttg agatcgatat catcaatgcg cgaagagagt
     2401 ttcgcagtgt agtagtaacg caggctgtcg gcgtcaaaat gattcagcca ggtgctggct
     2461 ttaataaagg taccgcgaga cttagacatc ttcgcgccgt tcaccgtcac atagccatga
     2521 acaaacaggt tggtcggctt gcggaagttg ctgccttcca gcattgctgg ccagaacaga
     2581 ctgtggaagt aaacaatatc tttaccgatg aagtggtaca gctcggcggt ggagtctttc
     2641 ttccagtatt catcgaagct tacgctgtcg ccgcgcttgt cgcacagatt cttgaaagaa
     2701 cccatatagc caatcggcgc atctagccag acgtagaaat atttacccgg cgcgttcgga
     2761 atttcaaaac cgaagtacgg cgcgtcgcgg gagatatccc actgttgcag gccagattca
     2821 aaccactcct gcattttatt tgccacctgc tcctgcaacg caccgctgtg gatccatgcc
     2881 tgcaacattt cgctgaaaga gggcagatca aagaagaagt gttcagaatc acgcattacc
     2941 ggcgtagcgc cagaaaccac cgatttcggc tcgatcaatt cagtcgggct gtaggtcgcg
     3001 ccgcagactt cgcagttatc gccgtattga tccggggatt tacatttcgg gcaggtgcct
     3061 tttacaaaac ggtccggcag gaacatgcct ttttccggat cgtacagctg agagatggtg
     3121 cggtttttaa taaaaccgtt ttctttcagg cgagagtaga taagttctga caactggcgg
     3181 ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga tgttaaagcc tgcgaaatca
     3241 gtctgatgct cctgactcat ttcgccaatc atctgctccg gggtgatacc aagctgctga
     3301 gctttcagca tgatcggtgt accgtgggca tcgtcggcgc agatgaagtt gacctcgtgg
     3361 ccgcgcattc gctggtaacg gacccagaca tcagcctgga tgtgctccag catatggccg
     3421 aggtggattg agccgttagc gtacggcagt gcgcacgtca ccagaatttt cttcgcgact
     3481 tgagtcatag taggcattac ttcttaatag tgaaaagggg ttagatagta ccaaatggga
     3541 aaatgttaag taagtacttc tctatattct tcaggtaaac tatcagctga tggtttatct
     3601 atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc aaatcaccag aagccttgcg
     3661 cgcaatggtc gccgggacac tggccaattt tcagcaccca accctgaagc ataacctcac
     3721 cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac acgttgcatg tggaactggt
     3781 tatgccattc gtctggcata gcgcgtttga agagttaaaa gagcaatgta gcgccgaact
     3841 gctgcgtatc accggggcga aagctatcga ctggaaactg tcgcacaata tcgccacctt
     3901 aaaacgcgtg aagaatcagc cgggtattaa tggcgtgaag aacatcattg cggtcagttc
     3961 cggtaaaggc ggcgtgggta aatcctccac ggcggtaaac ctggcgctgg cactggcggc
     4021 ggaaggggcg aaagtcggca ttctggacgc cgatatctac ggcccatcaa ttcccaccat
     4081 gctgggcgca gaaaatcagc gtccaacttc gccggacggt acccatatgg caccgattat
     4141 gtctcatggc ctggcaacca attcaattgg ttatctggtc actgacgaca atgcgatggt
     4201 gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg ctgcaggaaa ccttgtggcc
     4261 ggatctcgac tatctggtgc ttgatatgcc gccaggcacc ggtgacattc aattgacgct
     4321 ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc accccgcagg acatcgcgtt
     4381 gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc gaggttccgg tgctcggtat
     4441 tgtcgagaac atgagtgtgc atatttgcag taactgtggt caccatgaac cgattttcgg
     4501 taccggtggt gcagagaaac tggctgaaaa ataccataca cagctgctgg ggcagatgcc
     4561 gttacacatc tccttacgtg aggatctcga taaaggcacg ccaacggtta tcagccgtcc
     4621 ggagagcgaa tttaccgcta tctaccgcca gctggctgac cgcgttgcag ctcagctcta
     4681 ctggcagggt gaagtcattc caggcgagat ttccttccgc gcggtctaac gcctattaac
     4741 cattctggtt attcttcata caccgcagag atgttatatt gatgcggtgt atttgttttt
     4801 gccgcattaa tgaacactta tttaatactg cgaatattct gcggaccttc gtccgaaact
     4861 ctacaaagtc gattgatcgt acaggcaatg cttcattatc atccttcgtg gctgtgtagt
     4921 ggatgtacag ctatagtttg ttatgcactc attctatcca ggcataacct ttactacagg
     4981 gataacgttc aatgaataag tattggctgt caggtaatgc tgccaactta ctgatagtgt
     5041 tttatgttca gataatg
//
LOCUS       DAJTMK010000235         5022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTMK010000235 DAJTMK010000000
VERSION     DAJTMK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.240"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            64..1863
                     /gene="cysJ"
                     /locus_tag="OO135_003851"
     CDS             64..1863
                     /gene="cysJ"
                     /locus_tag="OO135_003851"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5399504.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLMNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLSDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            1863..3575
                     /gene="cysI"
                     /locus_tag="OO135_003852"
     CDS             1863..3575
                     /gene="cysI"
                     /locus_tag="OO135_003852"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5399505.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVDTFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3650..4384
                     /gene="cysH"
                     /locus_tag="OO135_003853"
     CDS             3650..4384
                     /gene="cysH"
                     /locus_tag="OO135_003853"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5399506.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            4649..4801
                     /locus_tag="OO135_003854"
     CDS             4649..4801
                     /locus_tag="OO135_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5399507.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
BASE COUNT         1251 a         1270 c         1403 g         1098 t
ORIGIN      
        1 cttctgtttt atgggcgctg acagggcgca gaaacagctt tgcttactgg aacataacga
       61 cgcatgacga cacaggtccc accttccgcg ttgcttccgt tgaacccgga gcaactggca
      121 cgccttcagg cggccacgac cgatttaacg cctacccagc ttgcctgggt ttctggctat
      181 ttctggggcg tactcaatca gcagcctgct gcgcttgcag cgacgccagc gccagccgca
      241 gaaatgccgg gtataactat tatctccgcc tcgcaaaccg gcaatgcgcg ccgggttgct
      301 gaagcattac gcgatgattt attagcagca aaactgaacg ttaagctgat gaacgcgggc
      361 gactataaat tcaaacaaat cgccagcgaa aaactgctca tcgtagtgac gtcaacgcaa
      421 ggggaagggg aaccgccgga agaagccgtc gcgctgcata agttcctgtt ctccaaaaaa
      481 gcgccaaagc tggaaaacac cgcgtttgcc gtgtttagcc tcggcgatag ctcttatgaa
      541 tttttctgcc agtccgggaa agatttcgac agcaagctgg cggaactggg tggtgaacgc
      601 ctgctcgacc gtgtcgatgc cgatgttgaa taccaggctg ctgccagcga gtggcgcgcc
      661 cgcgtggttg atgcgcttaa atcgcgtgcg cctgtcgcgg caccttcgca atccgtcgct
      721 actggcgcgg taaatgaaat ccacaccagc ccgtacagca aagacgcgcc gctggtggct
      781 agcctctctg ttaaccagaa aattaccggg cgtaactctg aaaaagacgt tcgccatatc
      841 gaaattgact taagtgactc gggcctgcgt taccagccgg gtgacgcgct gggcgtctgg
      901 tatcagaacg atccggcact ggtgaaagaa cttgtcgaac tgctgtggct gaaaggcgat
      961 gaacctgtca ccgtcgaggg caaaacgttg cctctgaacg aagcgctaca gtggcacttc
     1021 gaactgaccg tcaacaccgc caacattgtt gagaattacg ccacgcttac ccgcagcgaa
     1081 acactgctgc cgctggtggg cgataaagcg aagttacagc attacgccgc gacgacgccg
     1141 attgtcgaca tggtgcgttt ctctccggcg caactggatg ccgaagcgct gattaatctg
     1201 ctgcgcccgc tgaccccgcg cctgtattcc atcgcctcct cgcaggcgga agtcgagaac
     1261 gaagtacacg tcaccgttgg tgtggtgcgt tacgacgtgg aaggccgagc ccgtgccggt
     1321 ggtgcctcca gcttcctcgc ggatcgcgtg gaagaagagg gcgaagtccg cgtatttatc
     1381 gaacataacg ataactttcg cctgcccgct aacccggaaa ccccggtgat tatgattggc
     1441 ccaggcaccg gtattgcgcc gttccgcgcc tttatgcagc aacgcgccgc cgacgaagcg
     1501 ccaggtaaaa actggctgtt ctttggtaat ccgcacttta cggaagactt cctgtaccag
     1561 gtggagtggc agcgctacgt caaagatggc gtgctgacac gtatcgatct tgcctggtcg
     1621 cgcgaccaaa aagaaaaagt ttacgtacaa gacaaactgc gcgaacaggg cgcggagctg
     1681 tggcgctgga tcaatgatgg tgcccacatt tatgtctgcg gcgacgctaa tcgcatggcg
     1741 aaagacgttg agcaggcact tctggaagtg attgccgaat ttggtggcat ggacaccgaa
     1801 gcggcggatg aatttttaag tgagctgcgc gtagagcgcc gttatcagcg agatgtctac
     1861 taatgagcga aaaacatcca gggcctttag tggtcgaagg aaaactgaca gacgccgagc
     1921 gcatgaagct tgaaagcaac tacctgcgcg gcaccattgc ggaagattta aacgacggtc
     1981 tgaccggcgg ctttaagggc gacaacttcc tgctgatccg cttccacggc atgtatcagc
     2041 aggatgaccg cgacatccgc gccgaacgtg ctgaacagaa gctggagccg cgccacgcga
     2101 tgctgcttcg ctgtcgtctg ccgggtgggg tgattaccac taaacagtgg caggcgatcg
     2161 acaaatttgc cggtgaaaac accatctatg gcagcattcg cctgaccaac cgccagacat
     2221 tccagttcca cggcattctg aaaaagaacg tcaaaccggt gcaccagatg ctgcactcgg
     2281 tcggtcttga tgcgctggcg acagctaacg acatgaaccg taacgtactc tgcacctcga
     2341 acccttacga gtcgcagctg cacgcggaag cgtatgagtg ggcgaagaag atttctgagc
     2401 atctgttgcc tcgtacccgc gcgtatgcgg agatctggct cgaccaggaa aaagtcgcca
     2461 ctactgatga agaaccgatc cttggtcaga cctacctgcc gcgtaagttc aaaaccacgg
     2521 tagtgatccc gccgcagaac gatatcgatc tgcatgccaa cgacatgaac ttcgtggcaa
     2581 tcgccgaaaa cggcaagctg gtaggcttca acctgctggt gggcggtggg ctttctatcg
     2641 aacacggcaa caagaaaacc tacgcccgca cggcgagcga gtttggctat ctgccgctgg
     2701 agcatacgct ggcggtggcc gaagccgtcg tgacaactca gcgtgactgg ggtaaccgaa
     2761 ccgatcgtaa aaatgccaaa accaaataca cgctggagcg cgtgggggtt gatacgttta
     2821 aagcggaagt ggagcgtcgc gcggggatca aatttgaacc gatccgtcca tatgagttca
     2881 ccggacgagg cgatcgtatt ggctgggtta agggcattga tgataactgg cacctgacgc
     2941 tgtttatcga aaatggtcgc atccttgatt atccggggcg tccgctgaaa accggcctgc
     3001 tggagatcgc gaagatccac aaaggcgatt tccgcattac ggcgaaccag aatctgatca
     3061 tcgccggtgt accggaaagc gagaaagcga agatcgagaa gatcgccaaa gagagcgggt
     3121 taatgaatgc cgtcacgccg cagcgtgaaa actcgatggc ctgcgtgtca ttcccgactt
     3181 gcccgctggc gatggcggaa gcagagcgtt tcctgccgtc ttttatcgac aacatcgata
     3241 atttaatggc gaaacatggt gtcagcgatg agcatatcgt gatgcgtgta acaggctgcc
     3301 cgaacggttg tggtcgcgcg atgctggcgg aagtgggcct ggtgggtaaa gcgccgggtc
     3361 gctacaacct gcatcttggc ggcaaccgca ttgggacacg tatcccacgg atgtataaag
     3421 aaaacatcac cgagccggaa atcctggcgt cgcttgatga actgataggg cgctgggcga
     3481 aagagcgcga agcgggtgaa ggcttcggcg actttacggt gcgtgcgggc atcattcgcc
     3541 cggtgctcga tccggcgcgc gatttgtggg attaaccatc agcccggtcc tgtaggcctg
     3601 ataagaacgc gtgagcgtcg catcaggcaa ggcaaacagt gaggaatcta tgtccaaact
     3661 cgatctaaac gccctgaacg aactgccgaa ggtagatcgc attctggcgc tggcggaaac
     3721 taacgccgaa ctggaaaaac tggacgctga aggccgcgta gcctgggcgc tggataatct
     3781 gcccggtgaa tatgtacttt cttccagctt tggcattcag gcggcggtga gcctgcatct
     3841 ggtgaatcaa attcgcccgg atattccggt gatcctcacc gatacgggtt acttgttccc
     3901 ggaaacctac cgctttattg acgagttaac ggacaaactc aagctcaacc tgaaagtgta
     3961 ccgtgctacc gaaagcgcag cctggcagga agcacgctac ggaaaactgt gggagcaggg
     4021 cgttgaaggc attgaaaagt acaatgacat caacaaagtc gaaccgatga accgggctct
     4081 gaaagaactt aacgcgcaaa cctggtttgc tggcctgcgc cgtgaacaat ccggcagtcg
     4141 cgccaattta ccggtgctgg caattcagcg tggcgtattt aaagtgctgc cgattatcga
     4201 ctgggataac cgaactattt atcagtacct gcaaaaacat ggcctgaaat atcacccatt
     4261 atgggatgaa ggatatttat cggtcggtga tacccataca acccgtaaat gggaacctgg
     4321 catgtcggaa gaagaaacac gtttctttgg cttaaaaagg gaatgtggat tgcacgaagg
     4381 gtaaatattg ccggataaat taaacgcctc tgtcagaaat gatgggggcg tttttatttt
     4441 gcgagcagta ttcctgattt taaacgtgaa cgccgctgga agaattatca ggtatagaat
     4501 ggcgtcgggt gcttgaggct gtctggcgtt aagcattcgc gaggttccag atggacaaaa
     4561 gccccaggcg atatttctat caacctgagg ccagcgttcg aacccaaaca attcgaatgt
     4621 tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg tggcaatcat
     4681 agtgctgtgt tttacagtac tgggattcac gctgatggta ggcgactcgt tgtgtgagtt
     4741 gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat cgaagaagta
     4801 gccaccacgc ggggataaaa ccccgcgtac tgaattgtga tggtttggtc tcctgcaccc
     4861 ttttttaatt tatattgttt atgtgaatat tatgcctatg tttattattc atatatatta
     4921 cggatgaaaa ttatttgatt attcataatg taaatatttt attgaatgat atatatcaca
     4981 gataattttt aatatatgcg taaatatcct ttaagtttac tg
//
LOCUS       DAJTMK010000236         4994 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTMK010000236 DAJTMK010000000
VERSION     DAJTMK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.241"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..269
                     /locus_tag="OO135_003855"
     CDS             <1..269
                     /locus_tag="OO135_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCR5399508.1"
                     /translation="IGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYAQENVTID
                     MEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(310..780)
                     /locus_tag="OO135_003856"
     CDS             complement(310..780)
                     /locus_tag="OO135_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5399509.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(827..1546)
                     /gene="yehT"
                     /locus_tag="OO135_003857"
     CDS             complement(827..1546)
                     /gene="yehT"
                     /locus_tag="OO135_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5399510.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKTLKEAIGL"
     gene            complement(1543..3228)
                     /gene="btsS"
                     /locus_tag="OO135_003858"
     CDS             complement(1543..3228)
                     /gene="btsS"
                     /locus_tag="OO135_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5399511.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHIPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3450..3515
                     /locus_tag="OO135_003859"
                     /pseudo
     CDS             3450..3515
                     /locus_tag="OO135_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            3743..3991
                     /locus_tag="OO135_003860"
     CDS             3743..3991
                     /locus_tag="OO135_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5399512.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFFH"
     gene            complement(4095..4223)
                     /locus_tag="OO135_003861"
                     /pseudo
     CDS             complement(4095..4223)
                     /locus_tag="OO135_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            4308..4853
                     /locus_tag="OO135_003862"
     CDS             4308..4853
                     /locus_tag="OO135_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HCR5399513.1"
                     /translation="MNISELKKCIHYEVIGCKRPFSWRKAIVRAIKHRRSRYLFWWRI
                     AKYLFDKGGYRRKIAGKIERFILDKYNVTVPLTVNIGKGFDISYLNGVVIGHKVTIGE
                     NCSIKPGVTIGLRGEFNDMDIVIGNNVTIGCNATILGGKVHIGNNVKIGAHALVLHDI
                     PDDSTFITKFHSEIIYNSSHT"
     gene            complement(4864..>4994)
                     /locus_tag="OO135_003863"
     CDS             complement(4864..>4994)
                     /locus_tag="OO135_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5399514.1"
                     /translation="DAVDLEIATEEERSLLEAWNKYRVLVSRIDINTAPDIVWPEL"
BASE COUNT         1393 a         1229 c         1108 g         1264 t
ORIGIN      
        1 ctattggtgc aaccaccaaa gaagacgctg ccaagacact tgagccgtta agcgccaaat
       61 acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa
      121 acgtgactat cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca
      181 acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga
      241 aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc
      301 atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg
      361 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcacgca tcatcgccgt
      421 aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt
      481 tttcagcgaa aacgcgatgc gtaatttttt tagcacgatg ttgttattaa tgcgacgttc
      541 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
      601 gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
      661 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
      721 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
      781 gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
      841 cctcttttaa ggttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
      901 tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca
      961 gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt
     1021 cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga
     1081 caaatgccac atctttcatt tgcagcaaat aaatccgact atgcccagta caagggataa
     1141 atttcagcgc ctgttgattt tctggtaaca gcgaaacatc ctgcttgctg cgttcctgcc
     1201 gcaaacgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
     1261 aggcgtgttc ttcaaaggct ttaatggcgt attcgtcaaa cgcggtgaga aaaacaatat
     1321 acggacgatg ttccgggtcg agcatcccca ccatttccag accactgatg cgcggcatct
     1381 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
     1441 cgtttgaaca ctcaccaacg atttcaatat cgctctgctc ctgcaaaaat acgcgcaggt
     1501 tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
     1561 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttatcccata
     1621 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
     1681 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
     1741 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
     1801 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
     1861 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
     1921 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
     1981 gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
     2041 acggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgggggt tcacctgggc
     2101 gtgaagcagt ttgatctctg actgggtgag catcgctttt tgccgctcat attgcccggc
     2161 gaggatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt tgattgaact
     2221 gaataaacgg tttttggctt catacaattt gatggtgccc atcacccgct gattttcacc
     2281 acgcaacgga attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata
     2341 aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta
     2401 agtcgaagat atcggtttgc cgggtatatg gtggtcgtca ccaattccgg taaaggccag
     2461 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
     2521 tgccactttc atgctgttca cttcgttaaa cccctggcgc aaaatgcctt ccgtcgaggc
     2581 agccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
     2641 atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggcgc
     2701 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
     2761 caacatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
     2821 tttatcagtg cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc
     2881 aacgatggtc gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
     2941 cccgccggtc aggccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
     3001 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
     3061 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
     3121 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccatgcaa tgactaaaaa
     3181 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
     3241 cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc
     3301 agaaaaatga gaattcgtga gtacaatcac tcaaaatcgc ctggcaaaaa taaaatcacc
     3361 ctataaatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
     3421 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
     3481 tgctttgtga tattaaccct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
     3541 gaagaccgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
     3601 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
     3661 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
     3721 actacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
     3781 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
     3841 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
     3901 gcgcaccgcc gataaagatt cagctaaaac ttttgttcag gagactctga aagatacctg
     3961 gaagtctgct gacgagtggt tttttcacta attaacacgt aaaatcggta acggctggaa
     4021 atcattcaat actcgcacta tcgaaagttc gccagccagc cgtggcacgt tcttgcatac
     4081 gacgtgctac ggtttcattc atctccgacc ggaaacttct tatacaaagt cgatacgcca
     4141 acatcataga tgatcaccac cttctggcga gaaactcctg atgcaatttg tcgtccggcc
     4201 tgtgcctatt gctccggcgt gagagtaata cagtaaactg tagaaaaagc ccattaaacc
     4261 taatataata ataagttaca gaattatctt tactgaataa tagatatatg aatattagtg
     4321 aacttaaaaa gtgtatacac tatgaagtaa taggttgtaa acgccctttc tcctggcgaa
     4381 aagcaattgt tcgcgcaata aaacacagaa gatcacgtta tttattttgg tggcgcatag
     4441 caaaatatct ttttgataaa ggcggatacc ggcgaaagat tgcaggaaaa atagaacgct
     4501 tcattcttga taaatacaat gtaaccgttc ccttaactgt aaatattggt aaagggttcg
     4561 acatatctta tctcaatggc gttgttatcg gtcacaaagt tacaataggt gaaaattgtt
     4621 caattaagcc tggggtaact attggacttc gcggtgaatt taatgatatg gatattgtca
     4681 taggaaataa tgtaaccatt ggctgtaacg ccaccatcct tggtggcaaa gtgcatatag
     4741 ggaataatgt aaaaatagga gctcatgcat tggtactgca tgacattcct gatgactcca
     4801 cattcattac caaatttcac tctgaaatta tttataattc atctcacaca taattccgtt
     4861 taatcacagc tcaggccata cgatatccgg ggctgtattt atatcaatcc gactcactaa
     4921 tacccggtat ttattccatg cctccagcaa cgatctttct tcctccgttg cgatttccag
     4981 atctacagca tcct
//
LOCUS       DAJTMK010000237         4975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTMK010000237 DAJTMK010000000
VERSION     DAJTMK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.242"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(49..2853)
                     /locus_tag="OO135_003864"
     CDS             complement(49..2853)
                     /locus_tag="OO135_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5399515.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASDASASASEAATHATDAAASARAASTSAG
                     QAASSAQSASSSAGTASTKAREAAKSAAAAESSKSAAATSASAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARGAAASKEAAKSSETNASSSASSAASSATVAGNSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASASAAGKSAESAASSA
                     STATTKAGKATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTATMKASEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALT
                     GTPTTPTARQGTNNIQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTM
                     TNALAGKQPKDATLTALAGLTTAADKFPYFTGNDVASLATLTKVGRDILAKSTVAAVI
                     EYLGLRELGTSGEKIPLLSTANTWTNRQTFSGGLSGELSGNASTAAKLKTARKISNVA
                     FDGSSDITLKASHVGAFALGKTGSTVANDKAVGWNWSSGAYNATIRGASTLIIHFYMG
                     EGSCPAAQFRINYKNGGIFYRSARDGYGFEADWSEFYTTTRKPSAGDVGALPLSGGQL
                     NGALGIGTASALGGNSIVLGDNDTGFKQNGDGNLDVYANNVHVMRFVSGSIQSNKTIN
                     ITGRVNPSDYGNFDSRYVRDVRLGTRVVQTMKKGVMYEKSGHVITGLGIIGEVDGDDP
                     AVFRPIQKLINGTWYNVSQV"
     gene            complement(3004..3603)
                     /locus_tag="OO135_003865"
     CDS             complement(3004..3603)
                     /locus_tag="OO135_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5399516.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDLAYEGSGSGDWRTDAFIVGIGYRF"
     gene            complement(3671..>4975)
                     /locus_tag="OO135_003866"
     CDS             complement(3671..>4975)
                     /locus_tag="OO135_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR5399517.1"
                     /translation="YFQITATPHLAVYYPTVQFEFWFSEKRIADIRQVETAARYLGSA
                     LYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGEIGKTHL
                     AQELWTQIDNGQLAPDLAEIRTSITNVSNEITQTVNKKLENQSAAIQQIQKVQVDTNN
                     NLNSMWAVKLQQMQDGRLYIAGIDAGIENTPAGMQSQVLLAADRIAMINPANGNTKPM
                     FVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTL
                     NNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFD
                     RQIVIPAVAFSGAKHEKEHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAG
                     HGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATAGITIS"
BASE COUNT         1079 a         1382 c         1190 g         1324 t
ORIGIN      
        1 caacggtttt tggattacct gcgacaatgt tttttaaatg ctgcatgatt atacctgcga
       61 tacgttatac catgttccgt taattaactt ttgtattggt ctgaatactg ccggatcatc
      121 accatcgact tcaccgataa taccaagccc cgtgattaca tggcctgatt tttcatacat
      181 cacgcctttc ttcatggtct gaacaactcg tgtgccaagt ctgacatctc tcacatagcg
      241 ggaatcaaag ttaccgtaat ccgagggatt aacacgtccc gtaatattta tggttttatt
      301 actttgaatg cttccggaga caaagcgcat aacatggacg ttattagcat aaacatccag
      361 attaccatcg ccattttgtt taaagcccgt atcattatca cccaatacaa tcgaattacc
      421 gccaagagca ctggctgttc cgatacccag tgcaccattc agttgaccac cagataacgg
      481 cagtgcacca acatctcctg ctgaaggttt tctggtggtg gtgtaaaatt cggaccagtc
      541 ggcttcaaaa ccataaccat cacgggctga acgataaaaa ataccgccat ttttataatt
      601 aatccgaaac tgagctgcag gacaacttcc ttctcccata taaaaatgaa taattaacgt
      661 tgatgcacct ctaatagttg cgttataggc tccgctactc cagttccatc caactgcttt
      721 atcattcgca acggtgcttc ctgttttccc taaggcaaac gcaccaacat gacttgcttt
      781 taatgtgata tcggaggaac catcaaaagc cacattgctt attttcctgg cagtttttaa
      841 ttttgcagct gtagaagcat tgccggatag ttcaccagaa aggccaccgc tgaatgtttg
      901 tcgattagtc caggtattcg ctgtactgag taacggtatt ttctccccgc ttgtgccgag
      961 ttctcgtaaa ccgaggtatt cgataacggc agcaacagtc gatttcgcaa gaatatcccg
     1021 cccgactttt gtcagggttg ccaggctggc gacatcattc cccgtaaaat acggaaactt
     1081 gtctgccgca gtagtaagcc cggccagcgc cgtcagggtg gcatctttcg gttgcttacc
     1141 cgcaagcgcg ttagtcatgg tggtcgcaaa attcgggtcg tttcccagcg ccgccgctaa
     1201 ctcgttcagc gtattcagtg cgtcaggtga cgaatctaca agggcggcaa tcgcagccat
     1261 aacgaaagcc gtgcttgcga tttggatatt attcgttccc tgtcgcgcag ttggtgttgt
     1321 tggcgttccg gtcagtgcag gactatttaa gggcgctttc ttgttcgttt cacccattac
     1381 cgccttaacc gcttttggcg ttgccgccag tgactcggaa gtgctgttgg tcgcactgct
     1441 gagctgtact atcccctttt tcgtcgtgct cgcatcctcc agcgccacgg cggatgcaat
     1501 atcctctgcc cgttttgctg ctgtctcggc gcgcgttgcc gcggattccg ccgtactttt
     1561 gctctgagct gccgccgtcg cactaccagc tgcctctgat gctttcattg ttgctgtcgt
     1621 ggcactacct ttcgctgctg acgcttgtct ggtcgcctca tcttttgaag cagacgcaga
     1681 tgatgccgat gacgccgctg aactggctga cgatgcggca gccgccttag aggaagcagc
     1741 attgtctgct gaagtctttg catttgtttc agaggttttt gctgcagaag cagacctcgc
     1801 tgctgcagtg gcttgctcag tggctttgcc agccttcgtt gtggctgttg aagcggatga
     1861 tgcggcgctt tctgccgatt ttccggcggc gctagcactg gctgaggcct gcccggcact
     1921 tgttgacgcg gcactggcag acgacgcagc cgctgttttt gagcctgccg cagctgaggc
     1981 gctctgtccc gctgctgttt cagaagactt agcgttcgtc tcggacgttt ttgccgcctt
     2041 cgcggaattt cctgccaccg ttgccgagga agctgcacta ctggcacttg atgatgcatt
     2101 cgtttctgaa gatttcgctg cctcttttga agccgccgca ccccgtgccg aggtggcagc
     2161 ttctgacgcc ttcgtggtcg ctgtggatgc agaagtggct gccgattttt gtgatgctgc
     2221 ggcattcgtt tctgacgttt tcgcggcact ggcgctggta gctgccgcgc tttttgatga
     2281 ctctgcagca gcagcacttt ttgctgcttc acgggccttt gtcgatgccg ttcctgcgct
     2341 ggaagacgct gactgagccg acgacgcggc ctgtccggct gacgtgcttg cggcacgtgc
     2401 tgaggctgca gcatcggttg catgagttgc cgcctcgctg gctgatgcac tggcatcgct
     2461 ggctgatttt ttcgcggctg ccgtattctg tgcaaccgcg gaggcgttac gtgacacctc
     2521 ttccaccatc tgctcaaagc ggcgcagtgc ctccggtcgg acatcatcct ccgtcatggc
     2581 accgagaaaa tcatttagcg tacctggtct ggaaccttca tagacggtaa tggtcccggc
     2641 atgtgaaggc ggaaaacctt caaccagcag ggtgacgctg tactgaccat gctcaacatc
     2701 catgctgtaa cgtccggctt catccggatt ttcagaggcc accgtgttca ccaccaccgt
     2761 gctgctggtt cgtctggcct tcagcacaat ggtgcagttc tgtactggtt ttcctgtgcc
     2821 atctttaagc acgccagaaa tttttactgt catactttcc accaataaaa aaagcccgca
     2881 gcagtgacgc cacgggcttc aggacagtgt aactttacgt ttcctcaaac gcagttcacc
     2941 ccataaggtg gatgaacctg cgtatcataa caatatttac agaagataaa tcggcgtctg
     3001 ttgtcagaaa cggtatccga taccaacaat aaatgcatcc gttcgccagt cgccactacc
     3061 ggaaccttca taagcaaggt caacggccac ggattcggtc gggttaaact gcacgccagc
     3121 cccccacgcc agagaggtat tgctgtggcg accgtcatca cttccggtca gcacgtcgtg
     3181 cgttttcccc ttgttgtcag ttacgcggag ataatccccg gagaaagtcg acacacggct
     3241 gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgta cagacggccc
     3301 cgccatcaca ctgaaccagc ggttacgcac ggaatcttca tgccagcggg tatcgctgta
     3361 gcgcgttttt tgctcatcct cagcattggc ataactgaag gacgtaatca gccccagcgc
     3421 gtccgtaaac tcataacggt atttcacgtt aatcccgttc agatcatcac taccgggaac
     3481 gttcgtcgag gcatggagat accccgcgct cagcgtggac tgatgttctg ctgcactcgc
     3541 tggcgtagca gcggcgacct gccagactac tgcggacaaa ataacagcac ataatttacg
     3601 cataattacc tctcgctttt ctgcaataaa aaaggcgcca tttctggcgc ccgtatctgg
     3661 gttataaaat tcagctaatc gtgatgcctg cagtggcttt cttcatcacc acaaccagca
     3721 aatcgctgat acttgctgtg ggataccagt tatttaccag ccatgctgac accgaaaact
     3781 ccagtgtcat gtgaccgtga ccggcaggca tatcaataac accactgtaa atcagcgtat
     3841 tatccagcgc ggtacggtta taaatttcag caccgttttt ccgcactatc agacggcatg
     3901 aggagtaaat atcagtatgc tctttctcat gtttagcgcc gctgaatgcc accgccggaa
     3961 taacaatctg ccggtcaaac ggctgatcgt cataaaccct gacggtaatg gttcctgatg
     4021 gccaccgctc cggtgcacgg gagtcccggg ggaaagcttt gcccactgtt ttaacgagat
     4081 cgccttcaat ctggttcgcg gacaattttc ccagaacccg acagttctcg ttaatcgtga
     4141 cgttgttgag cgtcccggag ttcgcattca cgttaccgct gatatcggca tttttcgccg
     4201 tcagccgccc gtccggtgtc agggaaaatg ccggaggatt accgccgctg gtaatggtgg
     4261 gagccgtcag atatttcagg aacacttcat tcataaatat ctgatcgccc tgaccaacaa
     4321 acatcggctt tgtgttgcca ttcgcaggat taatcatcgc aatcctgtct gccgccagca
     4381 gcacctgact ctgcattcct gctggcgtat tctcaatacc ggcatcgata cccgcaatat
     4441 aaaggcgtcc gtcctgcatc tgctgcagtt tcacggccca catgctgttc aggttattat
     4501 ttgtatcaac ctgaactttc tgtatctgct ggattgccgc actctgattt tccagttttt
     4561 tattgacggt ctgcgtgatt tcattgctga cattcgtaat ggacgtcctg atttcagcca
     4621 ggtccggcgc aagctgaccg ttatcaatct gcgtccacag ctcctgggcc agatgtgttt
     4681 tcccgatttc tcctttgaag aaatccaggt aaccttccgc atcatcgctc gcccgaccga
     4741 cagcctccac gaatgccgat ttgccaacag tattcacact gcggatataa aaataataat
     4801 catggcccgg tttgatattg atactggcag ctatccagta cagcgccgag ccaagatagc
     4861 gggctgcggt ttcaacctgc ctgatatccg caatccgctt ttccgagaac cagaactcaa
     4921 actgtaccgt cgggtaataa acggcaagat gcggcgtggc ggttatctga aaata
//
LOCUS       DAJTMK010000238         4951 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTMK010000238 DAJTMK010000000
VERSION     DAJTMK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.243"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            239..805
                     /locus_tag="OO135_003867"
     CDS             239..805
                     /locus_tag="OO135_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5399518.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            856..3315
                     /locus_tag="OO135_003868"
     CDS             856..3315
                     /locus_tag="OO135_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5399519.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGVYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRSEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASNDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSTAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDHDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRHSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDNQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3330..4061
                     /locus_tag="OO135_003869"
     CDS             3330..4061
                     /locus_tag="OO135_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5399520.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4058..4951
                     /locus_tag="OO135_003870"
                     /pseudo
     CDS             4058..4951
                     /locus_tag="OO135_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT         1381 a         1205 c         1121 g         1244 t
ORIGIN      
        1 atcgatgatt tttaatcatc tatcttcctc tcagttacat gaataaacca ccaaatatag
       61 cgcaccccta taatttccaa tactttattc atcaaacatt taattcatac catcaattaa
      121 ttacttttac atttaataaa attaataata tttttaagaa aaactaaaaa cattcgacaa
      181 tcaaataaga tattcttgca ccgatatact tttttctttt ctgttaaagg actcagttat
      241 gtttaaagga caaaaaacat tggccgcact ggccgtatct ctgctgttca cagcacctgt
      301 ttatgcggct gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta ttgaagcacc
      361 gtgtgaaata catcaggatg atattgataa agaggttgaa ctcggtcagg tgaccaccag
      421 ccacattaat cagtcacatc acagcgatgc cgttgctgtc gacctgcgct tagtcaactg
      481 tgatctggaa aactccagca acggttccgg tggcaagatt tctaaagttg cagtcacctt
      541 tgatagctca gcgaaaacca ccggcgcaga tccgattctc aacaacacca gcacgggtga
      601 agccaccggc gtcggcgtac gtttaatgaa taaagatcaa agcaatatcg ttttaggcac
      661 cgctactcca gatatcgacc tggctccgac ctccagcgaa caaacgctga atttctttgc
      721 ctggatggaa caaattgatc aggctacacc tgtaacgcca ggcgcagtta cggcgaatgc
      781 aacctacgtg ctggattata aataagtatt actttggcgg cgctatcgcc gcctttcata
      841 aaatatttat caggtatgga caccgtgaat atttatcgac tctcttttgt atcctgcctg
      901 gtcgtggcga tgccttgcgc attggcggtc gaattcaacc ttaatgttct cgataaatcg
      961 atgcgcgacc gcattgatat ttcattatta aaggaaaaag gagtcattgc tcccggtgtg
     1021 tattttgtta gcgttgcggt aaataataac caaatcagta acgggcaaaa gattaactgg
     1081 cacaaaaatg acgataaaac cattccgtgc atcaatgatt tactggtcga taaatttggc
     1141 ttaaaacctg aagtccgtca gtcgttacca ttgataaatc agtgcgtcga ttttagctcc
     1201 cgatctgaaa tgctcttcaa tttcgatcaa gccaatcagc aactgaatat tagtattccg
     1261 caagcctggc tggcgtggca ctcagaaaac tggaccccac cctccacatg gaaagaaggt
     1321 gtcgccggtg tcctgatgga ttacaacttg tttgccagca gctaccgccc acaggatggc
     1381 agcagcagca ctaatcttaa cgcctatgga acagccggaa tcaacacggg ggcatggcgc
     1441 ttacgcagtg attatcagtt gaatcagact gatagcgatg ataaccatga acagtcaggc
     1501 gaaatatcac gtacctatct ttttcgcccg ttaccgcaat taggttcaaa attaaccctc
     1561 ggcgaaacgg attttagttc caatattttc gacggttttt cttataccgg tgcggcactg
     1621 gcaagtaacg atcgaatgtt gccatgggaa ctacgcggct acgccccaca aattagcggt
     1681 attgcacaga ccaatgccac ggtgacgatc agtcaatcag gccgcgtcat ttaccagaaa
     1741 aaagtcccac caggcccatt tatcattgac gaccttaatc agtctgttca gggcacactg
     1801 gatgtcaaag tgacggaaga agatggtcgg gtgaacaatt tccaggtttc ggcagcatcg
     1861 acgcccttcc tgactcgtca gggacaggtt cgctataaac tggccgcggg tcagccacgg
     1921 ccctccatgt cacatcaaac tgaaaatgaa acctttttta gcaatgaagt ttcctggggg
     1981 atgctgtcaa acacctcgct gtacggcggc ctgctgcttt ctggtgatga ctaccattct
     2041 accgcaatgg gtattgggca aaatatgctg tggcttggtg cgctgtcgtt tgatgtcacg
     2101 tgggccagta gccattttga tactcagcag gacgagcggg gcttaagcta ccgttttaat
     2161 tacagcaaac aagtggatgc cactaacagc acgatttccc tcgccgctta tcgtttctcc
     2221 gatcgtcatt ttcacagcta cgccaactat ctggatcaca aatacaacga cagcgatgcg
     2281 caggacgaaa aacagacgat cagcttatct gtgggccaac cgattacccc actaaacctc
     2341 aatctttacg ccaacctgct acatcaaacc tggtggaatg cagacgcctc cacgaccgcc
     2401 aacatcacgg caggttttaa tgttgatatt ggtgactgga gagatatctc tatttcgacg
     2461 tcattcaata caacccatta cgaagataaa gatcacgaca accagattta cctgtcgatt
     2521 tcgctcccct tcggtaacgg tggtcgggtt ggctatgaca tgcaaaacag tagccacagc
     2581 accacacacc gcatgtcgtg gaacgatacg ctggatgaac gtaatagctg gggcatgtct
     2641 gccggactgc aatccgaccg tcctgacaat ggagcccagg tgagcggcaa ctatcagcac
     2701 ctgagttcag cgggtgagtg ggatatttct ggtacctatg ccgccaatga ttacagttcc
     2761 gtcagcagca gctggagcgg ttctttcacc gcaacccaat atggtgcagc gtttcatcgc
     2821 cacagctcca ccaatgaacc acgcctgatg gtcagcaccg atggcgtggc agatattccg
     2881 gttcagggca atctcgacta caccaaccat tttggcattg cggtggtacc gttgatttcc
     2941 agttaccagc cttccaccgt ggcggtgaac atgaatgact tacccgacgg cgtaacagtt
     3001 gcagaaaacg ttatcaaaga aacgtggatt gaaggcgcga taggttacaa atcactggct
     3061 tcccgttccg gtaaagacgt taacgtcatc attcgcaacg ccagcggtca gtttcctccc
     3121 ctcggagcgg atatccgcca ggatgacagc ggcattagcg tggggatggt tggcgaggaa
     3181 ggacatgcct ggttaagcgg agtcgctgaa aatcaaaagt ttaccgtggt ctggggtgat
     3241 aaccagcatt gctcgctcca tcttcctgaa catatggaag acaccgcaaa tcgcctgatt
     3301 ttaccttgtc attaattaag gaaatgaaaa tgacatttat gaaaggactc ccccttttgt
     3361 tgttggttgc cagtctgtgc agccacgctg cactacaacc cgatcgcact cgtattgtgt
     3421 ttaacgctaa tgataaagct accagtctgc gggtggataa tcgtagcgat aaactcccct
     3481 acctcgctta ttcctggctc gaaaatgaaa aaggggagaa aagcgatgat cttctggtcg
     3541 ccctgccgcc gatacaacgt ctggaaccga aagcaacaac gcaggtacgg atagtaaagc
     3601 aagcctcgac cacaaaatta ccgggcgatc gcgaaacgct atttttctac aatatgcgtg
     3661 aaattcctcc cgccccggaa aaaaacagcg accatgccgt tctgcaagtc gccattcaga
     3721 gtcgtatcaa ggtgttctgg cgaccggctg cattacgcaa gaaagcagga gaaaaggtcg
     3781 aactgcagtt acaggtcagc cagcagggca accaactaac actgaaaaat cctaccgcgt
     3841 attatctgac cattgcctac ctgggacgaa atgaaaaagg cgttctcccc ggcttcaaaa
     3901 ccgtgatggt tgcgcctttt agtacggtca acacaaacac cggaaattat agcggcagcc
     3961 agttttatct cggttatatg gatgattacg gcgcgttacg catgacgacg ctcaactgca
     4021 gcggacaatg ccatttacaa gccgtggagg cgaagaaatg agtgctggca agggagtgtt
     4081 gctcgtcatc tgtctgttat ttctgccgtt gaagtctgcg cttgccctga actgctactt
     4141 tggttcatcc ggtggctcgg tagaaaaatc agaagcgatt caaccttttg ctgtaccagg
     4201 caatgccaaa ctcggcgata agatttggga atctgacgat attaaaattc ccgtctattg
     4261 tgacaacaat accaacggca attttgaaag tgagcatgtt tatgcctggg taaaccccta
     4321 cccgggggtg caggatcgct attatcaact cggcgtaact tacaatggcg ttgattatga
     4381 cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt attgacagta gaacattgac
     4441 atttataccc ctgagcagat tatcgcgatg ggatggcaga acaaaatttg ctcgggtgat
     4501 cccgctaata ttcacatgtc gcgtaccttt ctcgctcgca tgcggttata cgtcaaaata
     4561 cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg actatatcgt cgtgcaattt
     4621 gacggtgccg gtagcgttaa cgaagaccct actgcccaaa acctgaaata tcatattact
     4681 ggtctggaaa acattcgcgt gctggattgc agcgtcaatt tttccatttc accggaaaca
     4741 caggtgattg attttggtaa atttaatttg ctggatattc gtcgccacac aatgtcgaaa
     4801 acattcagta ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg atttaaagtc
     4861 agttcctcct tttataccga agaaacgttg gttgaagaag ataaagcatt gttgattggt
     4921 aatggtctga agctacgttt gctggatgaa a
//
LOCUS       DAJTMK010000239         4914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTMK010000239 DAJTMK010000000
VERSION     DAJTMK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.244"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(40..219)
                     /locus_tag="OO135_003871"
     CDS             complement(40..219)
                     /locus_tag="OO135_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5399521.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            293..1654
                     /gene="pabB"
                     /locus_tag="OO135_003872"
     CDS             293..1654
                     /gene="pabB"
                     /locus_tag="OO135_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5399522.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            1658..2236
                     /locus_tag="OO135_003873"
     CDS             1658..2236
                     /locus_tag="OO135_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5399523.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2420..3784
                     /gene="sdaA"
                     /locus_tag="OO135_003874"
     CDS             2420..3784
                     /gene="sdaA"
                     /locus_tag="OO135_003874"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5399524.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPKQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            3915..4673
                     /locus_tag="OO135_003875"
                     /pseudo
     CDS             3915..4673
                     /locus_tag="OO135_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
     gene            4736..>4914
                     /locus_tag="OO135_003876"
     CDS             4736..>4914
                     /locus_tag="OO135_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399525.1"
                     /translation="MSRKNQRYSKEFKAEAVRMVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTV"
BASE COUNT         1154 a         1271 c         1258 g         1231 t
ORIGIN      
        1 tcatcggctt ctataacgaa atcgatacgg attagtgtgt tactcgtctt catcctcaaa
       61 acgcgccacg attcgctcgc cggagtggtt ggcacgcagc tcttctgcca ccagcgcgat
      121 tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga cagctttttg
      181 ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct gctaaattgt
      241 tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac tgatgaagac
      301 gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat tttatttctc
      361 ccgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg atcatccgta
      421 tagccgcttt gatattgtgg tcgccgagcc gatttgcact ttaaccactt tcggtaaaga
      481 aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact gatgacccgc tacaggtgct
      541 ccagcaggtg ctggatcgcg cagacattcg cccaacgcat aacgaagatt tgccatttca
      601 gggcggcgca ctggggttgt ttggctacga tctgggccgc cgttttgagt cactgccaga
      661 aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg attgggcgct
      721 cattgtcgac caccagcgtc atacagtttc tttgctgagt cataatgatg tcaatgcccg
      781 tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa gatttcacgc tcacttccga
      841 ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg tacaggaata
      901 tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg cgacctattc
      961 tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc catttagcgc
     1021 ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt ttattctttg
     1081 tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc tgcccgatcc
     1141 tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc gtgccgaaaa
     1201 tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag ccggttcggt
     1261 aaaagtacca gagctcttcg tggtggaacc cttccctgcc gtgcatcatc tggtcagcac
     1321 tataacggcg cgactaccag aacagttaca cgccagcgat ctgctgcgcg cagcttttcc
     1381 tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg acgaactgga
     1441 accgcagcga cgtaatgcct ggtgcggcag cattggctat ttgagctttt gcggcaacat
     1501 ggataccagc attactatcc gcacgctgac tgccattaac ggacaaatat actgctctgc
     1561 gggcggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa cttttgataa
     1621 agttactaag atattacgcc aactggagaa gtaagacgtg gaataccgta gcctgacgct
     1681 tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa attaaccggg aaaccctaaa
     1741 tcatcgtcag gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg ggttgttgct
     1801 gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc ctggaggtgc
     1861 agtcgatgac acggacgcat cagttatcgc cgccgcgctg cgcgaagctg aagaagaggt
     1921 cgctataccg ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg atagcgtaac
     1981 tggctatcag gtaaccccag tggtcggcat tatcccgccc gatctgccgt atcgcgccag
     2041 tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc caggcattac atctgggtcg
     2101 ttatcaccct ttagatatct accgccgtgg cgattcacat cgggtatggc tgtcctggta
     2161 cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg cgctgcaaat
     2221 tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt gagacaatca
     2281 tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat agttaagcca
     2341 gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga agtccagtca
     2401 ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg ggattggtcc
     2461 ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg atgatctggt
     2521 cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg gttcactgtc
     2581 gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg caggtaacga
     2641 acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag aagagcgcga
     2701 acgtctgctg ctggcacagg gacggcatga agtggatttc cctcgcgaca acgggatgcg
     2761 ttttcataac ggcaacctgc cgctgcatga aaacggtatg caaatccacg cctataacgg
     2821 cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta tcgtcgatga
     2881 agaacacttt ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt tcaaatctgc
     2941 caccgaactg ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc tcgctatgca
     3001 gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc atgtctggca
     3061 aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc caggcccgct
     3121 gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgctg gtttccagcg ataaactgtc
     3181 taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag ttaacgaaga
     3241 aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg gtatcgttcc
     3301 ggcagtgctg gcttactatg accactttat tgaatcggtc agcccggaca tctatacccg
     3361 ttactttatg gcagcgggcg cgattggtgc attgtataaa atgaacgcct ctatttccgg
     3421 tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg ctgcgggtct
     3481 tgcagaactg ctgggcggta gcccgaaaca ggtttgcgtg gcggcggaaa ttggcatgga
     3541 acacaacctt ggtttaacct gcgacccggt tgcagggcag gttcaggtgc cgtgcattga
     3601 gcgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc tgcgccgcac
     3661 cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa ccggtaagga
     3721 catgaacgcc aaataccgcg aaacctcacg cggtggtctg gcaatcaaag tccagtgtga
     3781 ctaatacttc ttactcgccc atctgcaacg gatgggcgaa tttatacccg ctttctcgtc
     3841 tgctgtaata ttccccacta cacatccact gttgcgtcag gcgtttgtcg ccatacgctt
     3901 acagggtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcga cgtaatcgaa
     3961 tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc gtgcgattta
     4021 tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac gctgtagagg
     4081 aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt ccgctgattg
     4141 gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa ctccaaactg
     4201 tgcgatccat tggcctggtg caagacggca cactctattg ctccagcatt tttggttatc
     4261 gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca cttttacgcc
     4321 tgacgatcga ccgtgccctg attaaaggca gtccggtttt gattcaatgg acgcctgcag
     4381 cgggcagtag caatgctggg gtcatggaga tgattaacat cgacttactg acggcaatgc
     4441 tccttgagcc acaactgcag caaatcagta gcgccagcct gacggtggac aaacggcatt
     4501 tgctctatgg taatgggctg gtagattccc ttccgcaacc tgaagacaat gaaaactatc
     4561 aggtttcttc gcaacgcttt ccttttacca ttaacgttaa tggtccgggg gctacggcgc
     4621 tggcatggca ctatcttcca acacaattac cgctggcggt gctgctaagt ttgaggtagc
     4681 ctgagtttaa cggacactcc ttcctgaaat agaatggcat cagaaggagc taataatgag
     4741 cagaaaaaac caacgttact ctaaagagtt caaagccgaa gctgtcagaa tggttcttga
     4801 aaatcaactt tcgatcagtg aaggcgcttc ccgattatcc cttcctgaag gcactttagg
     4861 acaatgggtt accgccgcca gaaaagggct cggtactcct ggttcccgca cggt
//
LOCUS       DAJTMK010000240         4894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTMK010000240 DAJTMK010000000
VERSION     DAJTMK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.245"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..162
                     /locus_tag="OO135_003877"
     CDS             <1..162
                     /locus_tag="OO135_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5399526.1"
                     /translation="NESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAF
                     REKYHQMAA"
     gene            620..1870
                     /gene="frc"
                     /locus_tag="OO135_003878"
     CDS             620..1870
                     /gene="frc"
                     /locus_tag="OO135_003878"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5399527.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1924..3618
                     /gene="oxc"
                     /locus_tag="OO135_003879"
     CDS             1924..3618
                     /gene="oxc"
                     /locus_tag="OO135_003879"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5399528.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3688..4632
                     /gene="yfdV"
                     /locus_tag="OO135_003880"
     CDS             3688..4632
                     /gene="yfdV"
                     /locus_tag="OO135_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5399529.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4706..4849
                     /locus_tag="OO135_003881"
                     /pseudo
     CDS             4706..4849
                     /locus_tag="OO135_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
BASE COUNT         1304 a         1044 c         1146 g         1400 t
ORIGIN      
        1 aatgagagcc tgagccacta tcgttttaat aaccgggatg aagccatctc agtaatacgg
       61 gaatacattg agattttcta caatcgtcag cgtcgtcact ctcgtctggg gaatatctcc
      121 ccggcagcct tcagggaaaa atatcatcag atggctgctt aaaaaaagaa caaatggtag
      181 tgtccgctat tgccagtaca cctcagcgct cgcggttcta taaacacgca ttttatcttc
      241 ctcaaagtaa accccaataa tcccgtgtca tcacaaatca tttattatgt tatgttttat
      301 ggtacgcttt aaaaagcgta atcataccct tacttgtaaa tcaccggcgc ttcttacagt
      361 tgtaagaata acatcacaaa tgtcgcgact ctctctgatt accacgaatc aatatttatc
      421 atattcttct tttcgtcctc actacttacc atgcagaaag gaattggaag tggcgatgaa
      481 atgattttca gcacttgata caggatttta aaaatatcga gtgcagctac gacgatattt
      541 tgcgggttat ttatagaaga tattacgcct taaataaacg ctaaattaac acatatttaa
      601 ttaactaata ggtattgcta tgtcaactcc acttcaagga attaaagttc tcgatttcac
      661 cggtgtgcaa tctggcccat cttgtactca aatgctggcc tggtttggcg ctgacgttat
      721 taaaattgaa cgtcccggcg ttggtgacgt aacgcgtcac cagctgcgag atattcctga
      781 tatcgatgcg ctttacttca ccatgcttaa cagtaacaaa cgttctattg agttaaatac
      841 caaaacagcg gaaggcaaag aggtaatgga aaagctgatc cgcgaagctg atatcttagt
      901 cgagaacttt catccagggg ccattgatca catgggcttc acctgggagc atattcaaga
      961 aatcaatcca cgtctgattt ttggttcgat caaagggttt gatgagtgtt cgccttatgt
     1021 gaatgtaaaa gcctatgaaa acgttgctca ggcagcgggt ggcgcggcat ccactacggg
     1081 tttttgggat ggtccgccgc tggtaagcgc tgcagcgttg ggtgacagca acaccggaat
     1141 gcatttgctg atcggtttac ttgctgcttt gctgcatcgc gaaaaaacgg ggcgtgggca
     1201 acgagtcact atgtcaatgc aggatgccgt attgaacctt tgccgcgtga aattacgtga
     1261 ccagcagcgt ctcgataaat tgggttatct ggaagaatac ccgcagtatc cgaatggtac
     1321 atttggtgat gcagttcccc gcggtggtaa tgcaggtggt ggcggtcagc ctggctggat
     1381 cctgaaatgt aaaggctggg aaaccgatcc taacgcctat atttatttca ctattcagga
     1441 gcaaaactgg gaaaacacct gtaaagccat cggcaaacca gaatggatta ccgatccggc
     1501 atacagtaca gcccatgccc gacagccaca tattttcgat atttttgctg aaatcgaaaa
     1561 atacactgtc actattgata aacatgaagc ggtggcctat ttgactcagt ttgatattcc
     1621 ttgtgcaccg gttttaagta tgaaagaaat ttcacttgat ccctctttgc gccaaagtgg
     1681 cagtgttgtt gaagtggaac aaccgttgcg tggaaaatat ctgaccgttg gttgtccaat
     1741 gaaattctct gcttttacgc cggatattaa agctgcgccg ctattaggtg aacataccgc
     1801 tgctgtattg caggagctag gttatagcga cgatgaaatt gctgcaatga agcaaaacca
     1861 cgcaatctga tattaacggg ggcttctggc tcccaattta taaaaatttc gaggttatta
     1921 atcatgtcag atcaacttca aatgacagat ggtatgcata tcatcgttga agcattaaaa
     1981 cagaataata ttgacactat ttatggtgtt gtaggtattc ctgtgacgga tatggcacgc
     2041 catgcccagg cggaaggcat tcgttatatt ggttttcgtc atgagcagtc ggcaggctat
     2101 gccgctgcgg caagcggttt tcttacccaa aaaccgggga tctgcctgac agtttctgcg
     2161 ccaggattcc tcaatggttt gacggcattg gccaacgcaa cggtaaatgg ttttccgatg
     2221 atcatgatta gcggctccag cgaccgcgcg atcgtcgacc tacagcaagg tgattatgaa
     2281 gagctggacc aaatgaatgc ggcaaaaccg tatgccaaag cagcatttcg cgttaatcag
     2341 ccgcaggatc ttggcattgc attggcacgc gctatccggg tctctgtatc gggtcgccct
     2401 ggcggagttt atcttgattt gccagcaaat gtcctggccg cgacgatgga aaaagacgaa
     2461 gcgttaacca cgattgttaa agttgaaaat ccgtcgccag cattattgcc atgcccgaag
     2521 tcagtcacta gcgcaatttc gcttttagca aaagctgaac ggccattaat tatccttggc
     2581 aaaggcgcgg cgtattcaca agctgatgaa cagcttcgtg aatttattga aagtgctcag
     2641 attccattcc tgccaatgtc tatggcgaaa gggatccttg aagatacgca tccactttct
     2701 gcggcagctg cgcgttcgtt tgccctggca aatgctgacg ttgtcatgct tgttggtgca
     2761 cgactgaatt ggttattggc acacggtaaa aaaggatggg cggcagatac acagtttatt
     2821 caactggata ttgaaccgca ggaaattgac agcaaccgcc ccattgctgt gccagtcatt
     2881 ggtgatattg catccagtat gcaaggtatg ctggcagaac tgaaacaaaa cacatttacg
     2941 actccactgg tatggcgcga tattttaaat atccacaagc agcaaaatgc acaaaaaatg
     3001 catgaaaaat taagtacaga tactcaacca ttaaattact ttaatgcatt aagtgctgtg
     3061 cgcgacgtat tgcgcgagaa ccaggatatt tatttagtta atgaaggtgc aaataccctg
     3121 gataatgcac gaaatattat tgatatgtat aaaccacgtc gtcgtctgga ttgtggtacc
     3181 tggggtgtca tgggcatcgg tatgggctat gccatcggtg ctagcgtgac ttctggttct
     3241 ccggttgtcg ccattgaagg tgatagtgct tttggtttca gtgggatgga aattgaaacg
     3301 atttgtcgat ataacctgcc ggtgacgatc gttattttta ataatggcgg catctacaga
     3361 ggagacggtg ttgatctcag tggcgctggt gcaccatcac caacggatct gttgcaccat
     3421 gcaaggtatg acaaattaat ggatgcgttt cgtggcgttg gctataacgt caccacgaca
     3481 gatgaacttc gtcatgcttt aaccaccggt attcagtcgc gcaaaccgac cattattaat
     3541 gtggtcatcg accctgcagc aggaactgaa agtggccata ttaccaaact taacccaaaa
     3601 caagtcgctg gtaattaaat actgacatcg tctcctcttg ccaactcatt atgcgatggt
     3661 gaggggaccc ttatcgggaa tactattatg ctaacatttt ttattggcga tttattgcct
     3721 attatcgtaa tcatgctgtt gggttatttt agcggcagac gagaaacttt ttcagaagat
     3781 caagcccgcg catttaataa actggtatta aactacgcgc ttcctgcggc tctatttgta
     3841 tctattactc gggcaaacag ggaaatgatt tttgcggaca ctcgtctgac ccttgtatca
     3901 cttgtggtta ttgtcggatg tttctttttc tcctggttcg gttgctacaa attttttaaa
     3961 cgtacccatg cagaagcagc tgtatgtgca ttaattgcag gttcacctac cattggattc
     4021 ctggggtttg cagttctcga tcctatttat ggtgattccg tatcaacagg tttagtggta
     4081 gcaattattt ctattattgt taacgcaatt actattccta ttggtctgta tttgctgaat
     4141 ccttcttcag gagcggatgg taagaagaat agtaatctga gtgcattaat ttctgcggca
     4201 aaggagccag tagtatgggc acctgttctg gcaacgatcc tggtgttggt tggggtaaaa
     4261 attccggcag catgggaccc aacctttaat ctgattgcga aggctaactc aggggtagcg
     4321 gtattcgctg cggggttgac tctggctgca cataaattcg agttcagtgc cgaaattgct
     4381 tataacacct tcctgaagct gattctgatg ccactggcac tgcttctcgt tggtatggca
     4441 tgtcatttga acagcgaaca tctgcagatg atggtactgg caggcgcatt accgccggca
     4501 ttctccggaa ttattattgc cagccggttt aatgtctaca cccgtactgg tacagcgtca
     4561 ttggctgtga gcgtactggg ctttgtcgtc acggctccct tgtggattta tgtcagtcga
     4621 ctggtttcat aattaatcat gttattgatt ttacataaat gccgaagtgt cttttcacct
     4681 tcggcttttt tcaaggcata agcaaatgac aaataatgaa agcaaagggc cgtttgaagg
     4741 cttattagtt atcgatatga cacatgtcct taatggacct ttcggaactc aacttctttg
     4801 taatatgggc gcaagggtaa ttaaagttga gccgccgggt catggtgagg tagcctgagt
     4861 ttaacggaca ctccttcctg aaatagaatg gcat
//
LOCUS       DAJTMK010000241         4808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTMK010000241 DAJTMK010000000
VERSION     DAJTMK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.246"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            763..4808
                     /locus_tag="OO135_003882"
                     /pseudo
     CDS             763..4808
                     /locus_tag="OO135_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT         1292 a         1083 c         1298 g         1135 t
ORIGIN      
        1 tgacgggtta attatctgaa agaatttgtg aggctgtatc ggttactcat tgatttgata
       61 gttttactct cgggagagta atagatattt aatctattac tctcccgaga gtaatagata
      121 tttaatctat taacggaaac cagccagttc ctttcgatgc ctgaatttga ttccatagtt
      181 tatccggaat ggttaattca tgaataagtt gtgttctctt catgagaagc ataacgtaaa
      241 gggaaaagct cgattagacg gcaggatttg tcaggggtta tgaacgaaat tcataaatct
      301 gtttgagtgt tgcgatgagt agtgtaagtt cgatatcccc gcaatttaca gtccgatgaa
      361 ggaaaatgaa tatccataat aaatatatcg gtttatcctg gcgtatatac ctacttcgac
      421 gtatttccaa tagttgctaa ttaaaggcag gtcattatta ttcagtctga ataatgaatt
      481 attcactgtc cgcagagtaa gaaatataac ttaggtatct atttaatgac ttgcacaaaa
      541 agctaaattt tcacccataa ataaaaatat aatcccgcgc ccaaccacct gatgagtggc
      601 tacagacact ggatatatca ggtggcggtg cactttctta cataaaggta tttccttttc
      661 tgcggaaaag gaaaccggga aatccccggt ttttctgaca agcagacgcc attatttgtg
      721 tctgcctatg ttcgttaatt cgttcatcag gaaattatct caatgtcacg ttataaaaca
      781 ggtcataaac aaccacgatt tcgttattca gttctggccc gctgcgtggc gtgggcaaat
      841 atctctgttc aggttctttt tccactcgct gtcaccttta ccccagtaat ggcggcacgt
      901 gcgcagcatg cggttcagcc acggttgagc atgggaaata ctacggtaac tgctgataat
      961 aacgtggaga aaaatgtcgc gtcgtttgcc gcaaatgccg ggacattttt aagcagtcag
     1021 ccagatagcg atgcgacacg taattttatt accggaatgg ccaccgctaa agctaaccag
     1081 gaaatacagg agtggctcgg gaaatatggt acagcgcgcg tcaaactgaa tgtcgataaa
     1141 gatttctcgc tgaaggattc ttcgctggaa atgctttatc cgatttatga tacgccgaca
     1201 aatatgttgt tcactcaggg ggcaatacat cgtacagacg atcgtactca gtcaaatatt
     1261 ggttttggct ggcgtcattt ttcaggaaat gactggatga tgggggtgaa cacctttatc
     1321 gaccatgatt tatcccgtag tcatacccac attggtgttg gtgcggaata ctggcgcgat
     1381 tatctgaaac tgagcgccaa tggttatatt cgggcttctg gctggaaaaa atcgccggat
     1441 attgaggatt atcaggaacg cccggcgaat ggctgggata ttcgtgctga gggctattta
     1501 cccgcctgac cgcagcttgg cgcaagcctg atgtatgagc agtattatgg cgatgaagtt
     1561 gggctgtttg gcaaagataa acgccagaaa gatccacatg cgatttccgc tgaagtgaat
     1621 tacacgcctg tgcctcttct gaccctgagt gccgggcata agcagggcaa gagtggtgag
     1681 aatgacactc gctttggcct ggaagttaat tatcggattg gcgaacctct ggagaaacaa
     1741 ctcgatacag acagcattcg cgagcgtcga atgctggcag gcagccgcta tgacctggtt
     1801 gagcgtaata acaacatcgt tcttgagtac cgcaaatctg aagtgatccg tattgctctg
     1861 cctgagcgta ttgaaggcaa gggctgtcag acactttccc tcgggcttgt ggtcagcaaa
     1921 gcaactcacg gactgaaaaa tgtgcagtgg gaagcgccgt cattactggc tgaaggtggc
     1981 aaaattaccg gtcagggtag tcagtggcaa gtaacgctcc cggcttatcg tccaggcaaa
     2041 gacaattatt atgcgatttc tgcggttgcc tacgataaca aaggcaatac ctcaaaacgc
     2101 gtgcagacag aggtggtcat taccggagct ggtatgagcg ccgatcgcac ggcgttaacg
     2161 cttgatggtc agagccgtat tcaaatgctt gctaacggta atgaacaaaa accgctggtg
     2221 ctgtctctgc gcgacgccga gggccagcca gtcacgggca tgaaagatca gatcaagact
     2281 gaactaactt tcaaaccggc tggaaatatt gtgactcgtt ccctgaaggc cactaaatca
     2341 caggcaaagc caacactggg tgagttcact gaaactgaag ctggggtgta tcagtctgtc
     2401 ttcactaccg gaacgcaatc aggtgaggca acgattactg ttagcgttga tggcatgagc
     2461 aaaaccgtca ctgcagaatt gcgcgccacg atgatggatg tgtcgaagtc tactctgagc
     2521 gtgaatgaac tctcaggaga tgtggttgct gatggtcagc aatcccacac gctgacgctt
     2581 actgcggtgg atactgatgg taacccggtg acgggagagg ccagccgctt gcgatttgtt
     2641 ccgcaagaca ctaatggtgt caccattggt acaatttcgg agataaaacc aggcgtttac
     2701 agcgctacgg tttcttcgac ccgtgccgga aacgttgttg tgcgtgtgtg cgtgctttca
     2761 gcgagcagta tcagctgggc acattacaac aaacgctgaa gtttgttgcc gggccgcttg
     2821 atgcagcaca ttcgtccatc accctgaatc ctgataaacc ggtggttggg ggtacagtta
     2881 cggcaatctg gacggtaaaa gatgcctatg acaaccctgt gaccagcctc acgccggaag
     2941 cgccgtcatt agcgggtgcc gctgctgtag gttctacggc atcgggctgg acaaataatg
     3001 gtgatgggac gtggactgcg cagattactc tcggctctac ggcgggtgaa ttagaagtta
     3061 tgccgaagct aaatggacag gatgcggcag caaatgcggc aaaagtaacc gtggtggctg
     3121 atgcgttatc ttcaaaccag tcgaaagtct ctgtcgcaga agatcacgta aaagccagcg
     3181 aaagtacaac cgtgacgctg gtggcgaaag atgcgcatgg caacgctatc agtagtcttt
     3241 cgttgtcggc aagtttgacg gggaccgcct ctaaaggggc gaccgtttcc agttggaccg
     3301 aaaaaggtga cggttcctat gttgctacgt taactacagg cggaaagacg ggcgagcttc
     3361 gtgtcatgcc gctattcaac ggccagccag cagccaccga agccgcgcag ttgacggtca
     3421 tcgccggaga gatgtcatca gcgaactcta cgcttgttgc ggacaataag gctccgaccg
     3481 tcaaaacgac gacggaactc accttcaccg tgaaggatgc gtacaggaac ccggtcaccg
     3541 ggatgaagcc agatgcacca gtgtttagcg gtgccgccaa cacggggagt gagcgtcctt
     3601 cagcaggaaa ctggacagag aaaggtaatg gggtctacgt gtcgacctta acgctgggat
     3661 ctgccgcggg tcagttgtct gtgatgccgc gagtgaacgg ccaaaatgcc gttgctcagc
     3721 cactggtgct gaatgttgca ggtgacgcat ctaaggctga gattggtgat atgacagtga
     3781 aggttaataa ccaactggct aatggacagt ctgctaacca gataaccctg accgtcgtgg
     3841 acagctatgg taacccgttg caggggcaag aagttacgct gaatttaccg cagggtgtga
     3901 ccagcaagac ggggaataca gtaacaacca atgcggcagg taaagcggat attgagttga
     3961 tatcaaccgt tgcaggggaa cttgaaatcg cggccgcggt gaaaaactct cagaagacgg
     4021 tcacggtgaa attcaacgcg gatgccagca ccggtcaggc aaacctgcag gtagacaccg
     4081 ccgttcaaaa agtagcaaac ggcaaagatg cctttacgct gacggcgacc gttgaggata
     4141 aaaatggtaa ccctgttcca gggagcctgg tgacctttaa tctgccccgg ggtgtcaagc
     4201 cgcttacagg cgataatgtc tgggtgaaag ccaacgatga ggggaaagcc gagttgcagg
     4261 tggtttcagt gactgccgga acgtatgaga tcacggcatc ggcggggaat agccagcctt
     4321 cggatacgca gactataacg tttgtagccg ataaggctac cgcaaccgtc tccggtattg
     4381 aggtgattgg caactatgcg ctggcggacg gcaaagccaa acaaacgtat aaagttacgg
     4441 tgactgatgc caataacaat ttggtgaaag atagcgacgt gacgctgact accagcccgg
     4501 cttcgttaaa cctggaaccg aatggcactg cgacaacgaa tgagcaaggg caggctattt
     4561 tcaccgctac cactactgtt gcggcgacat acacactcaa ggcgcaagtg agtcagacca
     4621 acggtcaggt atcaacgaaa actgccgaat ctaaattcgt tgcggatgat aaaaacgcgg
     4681 tactcaccgc atcatctgat atgcaatctc tggtggcgga tgggaaatcg actgcgaagc
     4741 tggaggtgac actgatgtcg gcaaacaacc ccgttggggg gaatatgtgg gtcgacattc
     4801 agacgccg
//
LOCUS       DAJTMK010000242         4794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTMK010000242 DAJTMK010000000
VERSION     DAJTMK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.249"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..800
                     /locus_tag="OO135_003883"
     CDS             <1..800
                     /locus_tag="OO135_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HCR5399530.1"
                     /translation="SITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDA
                     NAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNATD
                     AVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYA
                     QLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIG
                     GGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1168..2823
                     /gene="lldP"
                     /locus_tag="OO135_003884"
     CDS             1168..2823
                     /gene="lldP"
                     /locus_tag="OO135_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5399531.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2823..3599
                     /gene="lldR"
                     /locus_tag="OO135_003885"
     CDS             2823..3599
                     /gene="lldR"
                     /locus_tag="OO135_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5399532.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3596..4786
                     /gene="lldD"
                     /locus_tag="OO135_003886"
     CDS             3596..4786
                     /gene="lldD"
                     /locus_tag="OO135_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5399533.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1054 a         1269 c         1301 g         1170 t
ORIGIN      
        1 agagcattac taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg
       61 aaaacggtat tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg
      121 atggcgtaga tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg
      181 ctgccgctga caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa
      241 tctctgtagg ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc ggtgttaatg
      301 ccaccgatgc ggttaacgtg tcgcagctga aatcttctga agcaggcggc gtgcgctacg
      361 acaccaaagc tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca
      421 gtacgactcg tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg
      481 cgcagttgaa gcaaagcgtg caggaaacga agcaatacac cgatcagcgg atggttgaga
      541 tggataacaa actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg
      601 caatgaccgg tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg
      661 gtacttacaa cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc
      721 gttgggtcta caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg
      781 gtgccggtat tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat
      841 tgcctgatgc gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc
      901 aattctctga tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc
      961 ctaccaattc ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca
     1021 atattgccaa tacataacat ttagttaacc attcattgtc gttatcccta cacaacacaa
     1081 ttggcagtgc cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg
     1141 atgtgcgtgg actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc
     1201 gggaatatct ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg
     1261 ctgattaagc tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt
     1321 gccgtggctt tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat
     1381 ggcttcttct atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat
     1441 aagatctcgg tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc
     1501 cctgaccagc gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga
     1561 gccgcaggct ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt
     1621 aaaccgctgt acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc
     1681 gcgatgggca ttccaatcct ggttgccgga caagtaacag gcatcgacag ttttgagatt
     1741 ggccagatgg tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg
     1801 gcgattatgg acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcgggc
     1861 ggctcgtttg ccatcgctca gtaccttagc tccaacttca ttgggccgga gctgccggac
     1921 attatctctt cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca
     1981 gttcgcgtat tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac
     2041 accggttaca ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct
     2101 accgtaacac tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg
     2161 tatgagtggg tgatcaacat tccggtgccg tacctcgata aactggttgc ccgtatgcca
     2221 ccagtggtca gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc
     2281 accggcaccg ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg
     2341 tctgacgcta tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc
     2401 atcggcatgg tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg
     2461 gcactggcac tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg
     2521 ctgggggtat tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa
     2581 gccaccgcag cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc
     2641 ggtggcgtca ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta
     2701 ggcctggtgg gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc
     2761 acctgtatgg tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct
     2821 taatgattgt tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga
     2881 ttgatgaaaa aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc
     2941 aactcggcgt atcacgtaat tcactacgcg aggcgctggc aaaactagtg agcgaaggcg
     3001 tgctgctcag tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg
     3061 agcaaaacat cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg
     3121 atattctgga agcccgctac gccattgaag ccagcactgc atggcatgcg gcaatgcgcg
     3181 ccacacctgg cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc
     3241 cggatatcgc ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata
     3301 acatcgtgct gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc
     3361 atagccgtca gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc
     3421 aggctgtcat tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg
     3481 cgcaccttag ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg
     3541 cacggattac ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat
     3601 tatttccgca gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt
     3661 ccactatatg gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt
     3721 gtcagaagtg gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac
     3781 gacgctgttt aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg
     3841 catgtatgcg cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc
     3901 gtttactctc tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg
     3961 cccaatgtgg ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga
     4021 gcgagcaaaa gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg
     4081 cgcacgctac cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta
     4141 cttgcaggcg gtgacgcatc cgcaatgggc gtgggatgta ggcctgaacg gtcgtccaca
     4201 tgatttaggt aatatctcag cttatctcgg caaaccaacc ggactggaag attacatcgg
     4261 ctggctgggg aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga
     4321 tttctgggat ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc
     4381 agtacgtttt ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg
     4441 tgtactctct tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc
     4501 cattctggcg gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg
     4561 tgccgacacc gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc
     4621 gggtgtagct aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac
     4681 tggcgcgaaa tcgatcagtg aaattacgca agattcgctg gtgcaagggc tgggtaaaga
     4741 gttgcctgcg gcactggctc ccatggcgaa agggaatgcg gcatagtcgt ttgc
//
LOCUS       DAJTMK010000243         4750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTMK010000243 DAJTMK010000000
VERSION     DAJTMK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.250"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..2311
                     /locus_tag="OO135_003887"
                     /pseudo
     CDS             1..2311
                     /locus_tag="OO135_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane usher protein"
     gene            2331..3083
                     /locus_tag="OO135_003888"
     CDS             2331..3083
                     /locus_tag="OO135_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5399534.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREILPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            3099..3608
                     /locus_tag="OO135_003889"
     CDS             3099..3608
                     /locus_tag="OO135_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399535.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            3605..4093
                     /locus_tag="OO135_003890"
     CDS             3605..4093
                     /locus_tag="OO135_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399536.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            4090..4629
                     /locus_tag="OO135_003891"
     CDS             4090..4629
                     /locus_tag="OO135_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399537.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLQSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
BASE COUNT         1183 a         1213 c         1324 g         1030 t
ORIGIN      
        1 tggcgaaaaa tctgcaatgg agccacgatg ctaaatgcct gaaatccggt caactggaag
       61 gcatggaaat taaggctgat ttaagccagt ccgcattagt catttcattg ccacaggctt
      121 acctcgaata tacttatccc gactgggatc cgccttcacg ttgggatgac ggcatctccg
      181 ggatcgtcgc ggactacagc atcaacgcac aaacccggca cgaagaaaat ggcggtgatg
      241 atagtaacga gatcagcggc aacgggacgg tcggggttaa cctggggccg tggcgtatgc
      301 gtgctgactg gcagactaac tatcaacata ctcgcagtaa tgatgacgat gaattcagcg
      361 gcgatgaaac tcaaaaaaaa tgggagtgga gtcgctacta tgcctggcgg gcgttaccat
      421 cattaaaagc caaactggcg ctgggcgagg attacctcag atccgatatt tttgatggtt
      481 ttaactatgt tggtggcagt gtcagtactg acgatcaaat gttgcctccc aatctgcgcg
      541 gctacgcgcc agacatttcc ggcgtggcac acaccacagc aaaagtgacc gtcagccaga
      601 tggggcgtgt gatttacgaa acgcaggttc cggctggacc gtttcgtatt caggatcttg
      661 gtgattctgt ctccggtacg ttgcatattc gcattgaaga acagaacggc caggtgcagg
      721 aatatgacat cagcaccgcc tcgatgccat acctcactcg tccaggtcag attcgctata
      781 agatcatgat gggccgtccg caagagtggg gacaccatgt cgagggtgaa ttttttctgg
      841 tgctgaagct tcctggggga tcgctaacgg ctggtcgtta tatggcggcg cactgggaga
      901 tgaaaactat cagtctgcgg cgcttggcgt cggtcgcgat ttgtctacat tcggcgcggt
      961 cgcgtttgat gttactcact cgcacaccaa actggataaa gacaccgctt atggcaaagg
     1021 ttcgctggac ggtaactcct tccgtgtgag ttattccaaa gactttgacc agctcaacag
     1081 ccgcgttact tttgctggat atcgcttctc ggaagagaac tttatgacca tgagcgagta
     1141 tctggatgcc agtgacagcg gaatggtacg cacgggcaac gacaaagaga tgtacaccgc
     1201 cacttataac cagaacttcc gcgatgcggg tgtttcggtt tatctcaact atacccgcca
     1261 tacctactgg gatcgcgagg agcagataaa ctacaacatc atgctctcgc actatttcaa
     1321 tatgggcagt attcgtaatg tcagcatctc gatgactggc taccgttacg agtatgacaa
     1381 ccaggccgac aaaggcatgt acatttcgct cagtatgccg tggggcgaca acagtaccgt
     1441 tagctataac ggtaactatg gcagtgggac ggacagcagt caggtcggtt atttcagccg
     1501 tgtcgatgac gcgactcact atcagttgaa cgtcggcacc agtgacaaac acaccagcgt
     1561 tgacggctgt tacagccatg atggttcgct ggcgcaggtt gacctcagtg cgaactacca
     1621 tgaagggcaa tacacctctg cgggcttgtc gttacagggc ggcgcaacgc ttactgccca
     1681 cggtggcgca cttcaccgta cccagaatat gggcgggaca cgcttgttga ttgatgccga
     1741 tggcgttgcc gatgttccgg tggaaggtaa cggggctgct gtttatacca atatgtttgg
     1801 taaagccgtc gtttctgacg tcaataacta ttaccgcaat caggcgtata tcgacctcaa
     1861 cagattgcct gaaaacgctg aagcaaccca gtcggtggtg caagccacgc taactgaagg
     1921 tgccattggc taccgtaaat ttgctgtcat tagcggacaa aaagcgatgg cggtgctgcg
     1981 cctgagcgac ggcagccatc ctccgtttgg cgcagaagta aaaaatgata acgagcagac
     2041 agtgggcctt gtcgatgatg acggcaatgt ttatctggct ggggtgaaac ctggcgaaca
     2101 catgagtgtg ttctggagtg gtgttgcgca ttgcgatatc aacctgccgg acccgctgcc
     2161 tgccgatctg tttaacggct tgttactgcc atgccagcat aaaggcaatg tagcacctat
     2221 cacttcgccg gcggtcaaac cggcgattca ggaacagaca cagcgggtga cgccaacgga
     2281 accaccgact tcaatttcag taaaccagta acgtgattaa ggaatgatac atgtttaatc
     2341 tgactaacac tgcaaaaatc gttgtcccgg cactggcgct gctggcgaca gcggtcagtt
     2401 tctccagcca cgcttcggtg acgcccgaca ggacacggct ggtgtttaac gaaagcgaca
     2461 agtccatcag cgtcacgctg cgcaacaacg atcccacact gccttatctg gcgcagagct
     2521 ggatagagga tgcaaagggc aacaaaatca gctcgccgct tgcggtgctt ccgccagtgc
     2581 agcgcatcga tgcgatgatg aacggccagg tgaaggtgca ggggctgccg gatatcaaca
     2641 aactgccggc cgaccgcgag agcgtgttct atttcaacgt gcgcgaaatt ctgccgaaat
     2701 caaataaacc gaacacactg cagattgcgc tgcagacgcg tatcaaactg ttctggcgac
     2761 cgaaggcgct ggagaacacc agcatgaaaa gtccgtggca gcataaggtg acgctcatcc
     2821 gtaacggcca ggacttcacc gtcaataacc cgacgccgta ctacgtgatc atcagcaacg
     2881 ccagcgctaa aaaaggcggc aacccgccgg cgggattcac accgctgatg atggagccga
     2941 aaacctccgc accgctgaat gcgaagatgg acagcgtgcc ggtgctgact tacatcaacg
     3001 actacggcgc gcggatgccg ctgtacttca aatgcaccgg caacagctgc caggtggatg
     3061 aagagcagag ccgcaaaggc tgatgccggg agggacttat gagtcacaag tttgtagcgg
     3121 gcgcagggtt gctggtgctg gccggatggg gcatcagcgg tttggctgtt gctggcagtg
     3181 atatcaaaca ggtggatctg acgctgcgaa tcctggtcga tggaccgccg ccttgctccg
     3241 tgaagggaag cgctgtgaaa tttggcaatg tgataatcaa caggatcgat ggtaccaatt
     3301 accaacagga tgcgaaatac accctcaact gcggtaataa agtcagcgat gacttacgga
     3361 tgcagctcaa aggcaatacc gccgtgatca acggagaaac ggttctcagc acggatatcg
     3421 ccggcctggg ggtgcggata gaaaacgccg ccgacaactc cctgtttgca gttggtgaaa
     3481 ataactggac gccttttaat gtcaacactc agcctcagct gaaagcggtg ccggtaaaac
     3541 agagcggggt gcaactgacg ccgtcggagt ttaacgccac gatgaccatg gtggtggatt
     3601 accaatgaaa aaattcctgc tccttttact gtgcgctggt tgcggggctg tgctggcggc
     3661 ggaagatgac atcacattcc acggtacgct gatgtcgccg cccgcctgta gcatcagcgg
     3721 cgggcaaacc atcgaagtgc aatttcgcga tctcattatc gacagcattg atggcaatta
     3781 tggtcgtcag aaagtggact acgaactgag ctgcgactcg gatgtccgcg atcccgagtg
     3841 gaatatgaca ctctcctgga cggggactgc cacctcctac aacgactctg ctattgagac
     3901 ggatgtaccg ggcttcggaa ttgagcttca gcatgacggt cagcgtttta agctcaacga
     3961 gccgctttcc attaacgcca ccgattttag ccagaagcca aagctggagg cggtaccggt
     4021 caaagcggct gatgcggtgc tgacggacac gaccttttct gcttacgcaa ccttacgggt
     4081 ggattaccaa tgaaaacagc acacatttat tcacccggtt atgcgctgtt agcaggcctg
     4141 ctgctgaccg ccagcacagg agcacaggca gtcgacaaca acctgcattt ttacggcaac
     4201 ctgcagagta aatcctgcac gctggtggtg gatggtgcca acctggcaga aatccatttt
     4261 cccaccgtca gcaaccggga tttgatggta tctgggcagt ccgcgcatct gccggttgtg
     4321 tttaagctga aagactgcaa agggccggcg ggatacaacg tcaaagtcac gctgagcggc
     4381 accgaagaca gcggtcagcc gggttttctg gcagtcgatg acacctccac cgcgcagggc
     4441 gtggggatag ggatggaaac gctcgacggc aagcgggtgg atattaacaa caccaccggg
     4501 gcgacctttg cgcttaccaa cggcaacaat gatattaatt tccgggcctg gatccaggcg
     4561 aaaagcggtc gtgatgtaac catcggtgat ttcaccgcca gcctgacagc aacttttgag
     4621 tatatttaaa atgaaaatcc ttcgtcatgt tttatgtctt ttattgctgt taatgttcaa
     4681 tacatgcggc caggcggcga cgaaagttgg tgagttatat gtgccagctg gtgccagttc
     4741 acctgcgaca
//
LOCUS       DAJTMK010000244         4741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTMK010000244 DAJTMK010000000
VERSION     DAJTMK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.251"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            229..450
                     /locus_tag="OO135_003892"
                     /pseudo
     CDS             229..450
                     /locus_tag="OO135_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(471..533)
                     /locus_tag="OO135_003893"
                     /pseudo
     CDS             complement(471..533)
                     /locus_tag="OO135_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            984..2396
                     /gene="ospC1"
                     /locus_tag="OO135_003894"
     CDS             984..2396
                     /gene="ospC1"
                     /locus_tag="OO135_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5399538.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLTPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHDLYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2500..2648)
                     /locus_tag="OO135_003895"
                     /pseudo
     CDS             complement(2500..2648)
                     /locus_tag="OO135_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2724..4421
                     /gene="ospD3"
                     /locus_tag="OO135_003896"
     CDS             2724..4421
                     /gene="ospD3"
                     /locus_tag="OO135_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5399539.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4538..4657
                     /locus_tag="OO135_003897"
                     /pseudo
     CDS             4538..4657
                     /locus_tag="OO135_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1638 a          865 c          882 g         1356 t
ORIGIN      
        1 aattatctaa ccttgtcttg ttacatccaa attgccatcg acagttacac agttgtgaag
       61 tcggctctct caaaaagggc ttataaaggc ttgagcggtg taccgggaaa ccggtatgca
      121 ccgttcttag gggagggcag tccagcaatg aactgtcctt acccgacaaa catccaaaac
      181 gaacggaacg tgctggaatc cgcatataac ccgttgtaaa gtgaggaaat ggacacctca
      241 cttgctcatg agaacgcccg cctgcgggca ctgttgcaga cgcaacagga cactatccac
      301 cagatggctg aatacaaccg cctgctctca cagcggatgg cggcttatgc ttccgaaatc
      361 aaccgactga aggcgcaggt tgcgaaactg cagcgtatgc agttcggtaa aagctcagaa
      421 aaacttcgcg caaaaaccga acggcagatt ccattttcac gggcgatcta cgccacacag
      481 gctctgggtt gcagtgactg ctccattaag gcgattttga actcataagg gaagtaacaa
      541 aaaaactgca aaagtacaat aattatatat ggccaaacta acctctatta aaaaaacatc
      601 agtttttcac acttagttga gcttcatgaa aaaatcatcg aggatttcat tgcagaatac
      661 acattccact aactggaagc actccataag aggaatcaga gaaagtatcg tttttttata
      721 gtaaaattca ttgctgttca ataaaattga tatagttatt aaattatgaa aataatttca
      781 caaaaaagat taacttgagt tcttaagcat cggattttag atattttgaa atatctggca
      841 tcacataaca tcaaaagtat ataataccct tatctaatta catgcagtat attgtatata
      901 tactcaaaaa ttgtaattca taccctacta tatcttgtgg ggaaagatat taattaaaac
      961 tgttttcata taaggttcat tttatgaata tatcagaaac actgaactca gcaaataccc
     1021 aatgcaatat agattctatg gataacagat tacatacatt gtttccaaaa gtgacatcag
     1081 tgcgaaacgc tgcacaacaa actatgccag atgaaaaaaa tttaaaagat agtgcaaata
     1141 ttattaaaga tttctttagg aaaactatag cagcacagag ttatagtaga atgttctctc
     1201 aaggctctaa ctttaaatct ttaaatatag caattgatgc accatcagac gctaaagcct
     1261 catttaaggc tattgagcac cttgacagat tatcgaagca ttatatatct gaaataaggg
     1321 aaaaacttca tcctctttct gcagaggaac tcaatttgct ttcgctaatt attaattctg
     1381 atttaatctt cagacatcaa agtaattctg atttgtctga taaaatttta aacattaagt
     1441 cattcaataa aattcagtct gaaggaatat gcacaaaacg aaacacatac gctgatgata
     1501 taaaaaaaat agctaatcat gactttgtgt tttttggcgt tgaaatctct agccatcaga
     1561 aaaaacaccc cctgaataca aaacatcaca ctgttgattt tggtgcaaat gcgtatatca
     1621 ttgatcatga ctctccatat ggatatatga cattaaccga tcactttgat aatgctattc
     1681 cacctgtttt ttaccatgag caccaatcat ttttagataa attttcagag gttaataaag
     1741 aagttagtcg atacgtacat ggaagtaaag gaattataga tgtaccaata ttcaatacta
     1801 aagatatgaa gttagggctc ggattatacc tgattgactt tattagaaaa agtgaagacc
     1861 aaagcttcaa ggagttttgc tatggaaaaa atcttacccc tgtggatctg gatagaatca
     1921 taaactttgt ttttcagcca gagtaccata tacctaggat ggtaagtaca gaaaacttca
     1981 aaaaagttaa gattagagaa atatccttag aggaggctgt tacagcatct aattacgaag
     2041 aaattaacaa gcaggtcact aacaaaaaaa ttgctctcca gactcttttt ctttcgatta
     2101 ctaatcaaaa agaggatgtc gccttatata tattatctaa ttttgagata actagacaag
     2161 atgttatttc cataaagcat gacttgtatg acattgagta tctacttagc gctcataatt
     2221 caagctgtaa agtacttgag tattttatca ataagggatt ggttgatgta aacacaaagt
     2281 tcaaaaaaac taatagtggg gattgtatgt tggataacgc aataaaatat gagaatgcag
     2341 aaatgataaa actattattg aaatatggtg caacatctga caataaatat atttaatcaa
     2401 aattgaatat cgtttagcaa aaggcagtat ttgcacaaaa tatcgatata ttgtttattg
     2461 tcagtatggc tcaatgtgat aatagttgga aagtttgatg ggtttcgccc cgttgtagcg
     2521 gtagtcgagc tggaaggtct tcaggcactg cttacagcga tagagcagcc cccctgaact
     2581 ggaatggccg ttccggatac cccctgagtt tcagagtaac ggggacaaac cacatcaatc
     2641 tttgccatca atcatccaaa gggcaaagag tacaacaaca ctaagtctgc gtcacaaccc
     2701 atcaatgaaa ggaatatata catatgccat cagtaaattt aatcccatca aggaaaatat
     2761 gtttgcaaaa tatgataaat aaagacaacg tctctgttga gacaatccag tctctattgc
     2821 actcaaaaca attgccatat ttttctgaca agaggagttt tttattaaat ctaaattgcc
     2881 aagttaccga tcactctgga agacttattg tctgtcgaca tttagcttcc tactggatag
     2941 cacagtttaa caaaagtagt ggtcacgtgg attatcatca ctttgctttt ccggatgaaa
     3001 ttaaaaatta tgtttcagtg agtgaagaag aaaaggctat taatgtgcct gctattattt
     3061 attttgttga aaacggttca tggggagata ttatttttta tattttcaat gaaatgattt
     3121 ttcattccga aaaaagcaga gcactagaaa taagtacatc aaatcacaat atggcattag
     3181 gcttgaagat taaagaaact aaaaatgggg gagattttgt cattcagctt tatgatccca
     3241 accatacagc aactcattta cgagcagagt ttaacaaatt taacttggct aaaataaaaa
     3301 aactgactgt agataatttt cttgatgaaa aacatcagaa atgttatggt cttatatccg
     3361 acggtatgtc tatatttgtg gacagacata ctccaacaag catgtcctcc ataatcagat
     3421 ggcctaataa tttacttcac cccaaagtta tttatcacgc gatgcgtatg ggattgactg
     3481 agctaatcca aaaagtaaca agagtcgtac aactatctga cctttcagac aatacgttag
     3541 aattactttt ggcagccaaa aatgacgatg gtttgtcagg attgctttta gctttacaaa
     3601 atgggcattc agatacaatc ttagcatacg gagaactcct ggaaacttct ggacttaacc
     3661 ttgataaaac ggtagaacta ctaactgcgg aaggaatggg aggacgaata tcgggtttat
     3721 cccaagcact tcaaaatggg catgcagaaa ctatcaaaac atacggaagg cttctcaaga
     3781 agagagcaat aaatatcgaa tacaataagc tgaaaaattt gctgaccgct tattattatg
     3841 atgaagtaca cagacagata cccggactaa tgtttgctct tcaaaatgga catgcagatg
     3901 ctatacgcgc atacggtgag ctcattctta gcccccctct cctcaactca gaggatattg
     3961 taaatttgct ggcctcaagg agatatgaca atgttcccgg acttctgtta gcattgaata
     4021 atggacaggc tgatgcaatc ttagcttatg gtgatatatt gaatgaggca aaacttaact
     4081 tggataaaaa agcagagctg ttagaagcga aagattctaa tggtttatct ggattgtttg
     4141 tagccttgca taatggatgt gtagaaacaa ttattgctta tgggaaaata cttcacactg
     4201 cagaccttac tccacatcag gcatcaaaat tactggcggc agaaggccca aatggggtat
     4261 ctggattaat tatagctttt caaaatagga attttgaggc aataaaaact tatatgggaa
     4321 taataaaaaa tgaaaatatt acacctgaag aaatagcaga acacttggac aaaaaaaatg
     4381 gaagtgattt tctagaaatt atgaagaata taaaaagctg aatattatct cgtggtgata
     4441 cactgaattt gaggtaaccg gctcatttaa accgtctggt ctgttacctc cggttccaca
     4501 aaaataatgt ccatcatttt gaagtggaca ctatcgtatg gaataccgga cctggatcac
     4561 tgaagcttta cgccttcatt tcgaagaaca tttaccacgg gttgtggccg gacgtcgcct
     4621 gggcgtacca aaatcaactg tctgtggtat gttcgtgtga accgcccccg gtatcccgct
     4681 catgctggcg aacccagaca cgcagtgaac cgccccgggt ttcctggaga gtgttttatc
     4741 t
//
LOCUS       DAJTMK010000245         4693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTMK010000245 DAJTMK010000000
VERSION     DAJTMK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.252"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..408)
                     /gene="dsbG"
                     /locus_tag="OO135_003898"
     CDS             complement(<1..408)
                     /gene="dsbG"
                     /locus_tag="OO135_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5399540.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREIWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQA"
     gene            780..1343
                     /gene="ahpC"
                     /locus_tag="OO135_003899"
     CDS             780..1343
                     /gene="ahpC"
                     /locus_tag="OO135_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5399541.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1514..3079
                     /gene="ahpF"
                     /locus_tag="OO135_003900"
     CDS             1514..3079
                     /gene="ahpF"
                     /locus_tag="OO135_003900"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5399542.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3200..3628)
                     /gene="uspG"
                     /locus_tag="OO135_003901"
     CDS             complement(3200..3628)
                     /gene="uspG"
                     /locus_tag="OO135_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5399543.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3849..>4693
                     /locus_tag="OO135_003902"
     CDS             3849..>4693
                     /locus_tag="OO135_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR5399544.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNL"
BASE COUNT         1214 a         1132 c         1195 g         1152 t
ORIGIN      
        1 cgcctgctgc cagaactgtt tacaatatgg gcagaacgga tcggcgaaga cgtagacaat
       61 gaccggcgca tcttttttac cgtcgaggag ccagtgggat tgttccatcc gttgccatat
      121 ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt gtgttactca ggttttcacc
      181 tttctcgttg tacatgtaac cagagatagc gtgcttacca tctggagtca ggtagatggt
      241 gacgcccata tcctgatact ttccgagata acctttcatt cctccggggg catcgaacgt
      301 tttgatgatt gtaatgccct gtttttcaat cgcttttact ggagcaggaa gttcctctgc
      361 gaaggcgatt gcaggaagca gagccagtaa aagtatcttt tttaacatta atttgtcctt
      421 ttcagtcagt gcaaaagtcg agtaaaaggc ataacctatc actgtcatag gtaagagctt
      481 agatcaggtg attgcccttt gtttatgagg gtgttgtaat ccatgtcgtt gttgcatttg
      541 taagggcaac acctcagcct gcaggcaggc gctgaagata ccaaagggta gttcagatta
      601 cacggtcacc tggaaagggg gccattttac tttttatcgc cgctggcggt gcaaagttca
      661 caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga tttgataatg gaaacgcatt
      721 agccgaatcg gcaaaaattg gttaccttac atctcatcga aaacacggag gaagtataga
      781 tgtccttgat taacaccaaa attaaacctt ttaaaaacca ggcattcaaa aacggcgaat
      841 tcatcgaaat caccgaaaaa gataccgaag gccgctggag cgtcttcttc ttctacccgg
      901 ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt tgctgaccac tacgaagaac
      961 tgcagaaact gggcgtagac gtatacgcag tatctaccga tactcacttc acccacaaag
     1021 catggcacag cagctctgaa accatcgcta aaatcaaata tgcgatgatc ggcgacccga
     1081 ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga tgaaggtctg gctgaccgtg
     1141 cgaccttcgt tgttgacccg cagggtatca tccaggcaat cgaagttacc gctgaaggca
     1201 ttggccgtga cgcgtctgac ctgctgcgta aaatcaaagc agcacagtac gtagcttctc
     1261 acccaggtga agtttgcccg gctaaatgga aagaaggtga agcaactctg gctccgtctc
     1321 tggacctggt tggtaaaatc taaatttcct tcgtctttcg cgctaccaac tccggcgcga
     1381 aatacttcgg aaattcacct aattcttcgg gtgctgcggc acccgatttt cttccccgca
     1441 ctatgatgca agctgcatcc aggtagccgt agaggccgct tgcatgatga tgtttaaagc
     1501 ccaggagata aacatgctcg acacaaatat gaaaactcaa ctcaaggctt accttgagaa
     1561 attgaccaag cctgttgagt taattgccac gctggatgac agcgctaaat cggcagaaat
     1621 caaggaactg ttggctgaaa tcgcagaact gtcagacaaa gtcaccttta aagaagataa
     1681 cagcttgccg gtgcgtaagc cgtctttcct gatcaccaac ccaggttcca accaggggcc
     1741 acgttttgca ggctccccgc tgggccacga gttcacctcg ctggtactgg cgttgctgtg
     1801 gaccggtggt catccgtcga aagaagcgca gtctctgctg gagcagattc gccatattga
     1861 cggtgatttt gaattcgaaa cctattactc gctctcttgc cataactgcc cggacgtggt
     1921 gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc aagcacactg caattgacgg
     1981 cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg ggcgttccgg cagtgttcgt
     2041 aaacgggaaa gagtttggtc agggccgcat gacgttgact gaaatcgttg ccaaaattga
     2101 tacgggcgcg gaaaaacgtg cggcagaaga gctgaacaag cgtgatgctt atgacgtatt
     2161 aatcgtcggt tccggcccgg cgggtgcagc ggcagcaatt tactccgcac gtaaaggcat
     2221 ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc ctcgataccg ttgatatcga
     2281 aaactacatt tctgtaccga agactgaagg gcagaagctg gcaggcgcac tgaaagttca
     2341 cgttgatgaa tacgacgttg atgtgatcga cagccagagc gccagcaaac tgatcccagc
     2401 agcagttgaa ggtggtctgc atcagattga aacagcttct ggcgcggtac tgaaagcacg
     2461 cagcattatc gtggcgaccg gtgcaaaatg gcgcaacatg aacgttccgg gcgaagatca
     2521 gtatcgcacc aaaggcgtga cctactgccc gcactgcgac ggcccgctgt ttaaaggtaa
     2581 acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa gcggcaattg acctggcggg
     2641 tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa atgaaagccg accaggttct
     2701 gcaggacaaa ctgcgcagcc tgaaaaacgt cgacattatt ctgaatgcgc aaaccacgga
     2761 agtgaaaggc gacggcagca aagtcgttgg tctggaatat cgagatcgtg tcagcggcga
     2821 tattcacaac atcgaactgg ccggtatttt cgtgcagatt ggtctgctgc cgaacaccaa
     2881 ctggctcgaa ggcgcagtcg aacgtaaccg catgggtgag attatcattg atgcgaaatg
     2941 cgaaaccaac gtcaaaggcg tattcgcagc gggtgactgt acgacggttc cgtacaagca
     3001 gatcatcatc gccactggcg aaggtgccaa agcctctctg agtgcttttg actacctgat
     3061 tcgcaccaaa actgcataag aagaagtaag attcacctgc aattgcttag ccgccggggt
     3121 caaacctagc ggctttttta tggcattaaa aagccctgcc gggatgttcc aggcagggcg
     3181 gcataaatac ttttatctgt tagcgaacga ccagtacagg cagattggcg tggcggatca
     3241 cgctcgaggc gttagaacct aacagatggg tcgaaatcga tgggttgcga gaaccgataa
     3301 ctacaacatc agcccccagt tcttctgcca actcattgac ttcatcccgc acgctaccaa
     3361 aacggacatg ttgtttaatg cgggaaggat cgatggtgaa gtggctgacc atcgtttgca
     3421 gacgttcttc tgcttcatgt tgcagatgct cttcaaaacg acgcacatca gcggcaaaac
     3481 ggtgcaggct caggctggct gacccgggta gtacgtgaag tagatgaata actccgtcat
     3541 cctgggtgag gaattcagcg tggcgaacag ctttgtcgct caattccatt tcaaatacat
     3601 caactggcat aatgattgtc ttatacataa ccctttctcc ctgttaatca tgaacaaatc
     3661 attcgccatg attataatat ttatccctga tatttgtctg gttctttttc cttacgaacg
     3721 gtttctgtga ttaatatatt ctcactgaac accaggaatt ctcccaaaac ctgcggtacc
     3781 gcccgttttc ccgctgtgat agctaccctt aaagactgac tcttttttga actgtctctg
     3841 gaggttgcat gaaagcattg acttatcacg gcccacatca cgttcaggta gaaaatgttc
     3901 ccgatccggg cattgaacag gcagatgata ttattctgcg tattacggca acggcgatct
     3961 gtggctctga tctccatctt tatcgaggca aaatacccca ggttaaacat ggcgatattt
     4021 ttggtcatga atttatgggg gaagtcgttg aaaccggaaa ggacgtaaaa aatttgcaaa
     4081 aaggcgaccg ggtggtaatt ccgtttgtca ttgcttgtgg cgactgtttt ttctgtcgaa
     4141 tgcagcaata tgccgcctgc gaaaatacca atgcgggtaa aggcgctgcg ctcaataaaa
     4201 aacagatacc agctcccgcg gcattgtttg gttatagtca cctgtatggt ggcgttcctg
     4261 gtggacaggc ggaatatgtc cgcgtcccta aagggaatgt ggggccgttt aaagtaccgc
     4321 ctttgctttc agatgataaa gcccttttcc tttctgatat tctgccaacg gcatggcagg
     4381 cagcaaaaaa tgcgcagatc caacaaggtt caagcgttgc agtctatggt gccggtccag
     4441 tgggattgtt gacaatcgcc tgtgcacggt tgctcggtgc ggaacagatt tttgttgttg
     4501 atcatcatcc ctaccgcttg agtttcgccg ctgaccgcta cggcgcgatc ccgattaatt
     4561 tcgatgaaga tagcgatccg gcacagtcaa ttattgaaca aacggcaggt caccggggcg
     4621 tggatgcagt aatagacgcc gtcggttttg aagcgaaagg cagcaccacg gaaacggtgc
     4681 tgactaatct gaa
//
LOCUS       DAJTMK010000246         4656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTMK010000246 DAJTMK010000000
VERSION     DAJTMK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.253"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            308..643
                     /locus_tag="OO135_003903"
     CDS             308..643
                     /locus_tag="OO135_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5399545.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            803..1246
                     /gene="yjdN"
                     /locus_tag="OO135_003904"
     CDS             803..1246
                     /gene="yjdN"
                     /locus_tag="OO135_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5399546.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSYIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEIAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1379..2167
                     /gene="phnC"
                     /locus_tag="OO135_003905"
     CDS             1379..2167
                     /gene="phnC"
                     /locus_tag="OO135_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5399547.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPITSLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2192..3208
                     /gene="phnD"
                     /locus_tag="OO135_003906"
     CDS             2192..3208
                     /gene="phnD"
                     /locus_tag="OO135_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5399548.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWMPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRT
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3334..4113
                     /gene="phnE"
                     /locus_tag="OO135_003907"
     CDS             3334..4113
                     /gene="phnE"
                     /locus_tag="OO135_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5399549.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4134..>4656
                     /gene="phnF"
                     /locus_tag="OO135_003908"
     CDS             4134..>4656
                     /gene="phnF"
                     /locus_tag="OO135_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5399550.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQ"
BASE COUNT         1126 a         1338 c         1196 g          996 t
ORIGIN      
        1 aattttatat ttagtgtgtt gttttttatc agaataaata acgtcttctg atgcgtttaa
       61 aacgtcagaa agataaaaat atcatgtgaa taaaaaaaga acaagtagag cattaacatt
      121 atcttaaata ataaatagag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
      181 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      241 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      301 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
      361 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
      421 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      481 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      541 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      601 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      661 agccggataa ggcgctcgcg ccacatccgg caacgatgcc gactgcctga tgcgacgctg
      721 accgcgcctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct
      781 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      841 ttccgacgcg attgcctatt atcaacgtac gttgggcgcg gaactgctct ataaaatcag
      901 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      961 atttcccgat accgccatcg cccacgccaa cgtgcgcatt gccggaagct acatcatgat
     1021 gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
     1081 gcaacaggtc gaagaaggaa aacgctggtt tgataacctt gccgctaacg gaaaaattga
     1141 aatagcctgg caggaaactt tctgggcgca tggcttcggc aaagtcaccg ataaatttgg
     1201 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc
     1261 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1321 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1381 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aagcgctgca
     1441 tgcggttgat ctgaacattc atcacagtga aatggtggct ctgcttgggc cgtcgggttc
     1501 cggcaaatcc acacttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1561 cagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat
     1621 ccgcaaaagc cgcgcccata ccggctacat cttccaacaa ttcaacctgg tgaatcgcct
     1681 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1741 ttttagctgg ttcacccgcg agcagaaaca acgcgcgtta caggcgctga cccgcgttgg
     1801 catggtgcat tttgcccatc aacgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1861 ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcac
     1921 ctcgctggac ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa
     1981 cgacggcatc accgtagtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     2041 acgcatcgtc gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga
     2101 taacgaacgt tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc
     2161 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2221 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2281 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg
     2341 gatgccattc ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc
     2401 cccagactac gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta
     2461 cggcaacctg tcggcaatgg aagcggtgga tcgcaccaac ggccaggtct tcgcccagac
     2521 ggtcgcggcg gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc
     2581 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga
     2641 tcctaactcc acctctggct tcctagtccc cggttactac gtctttgcca aaaacaatat
     2701 ctccgccagc gactttaagc gcaccgtcaa cgctgggcat gaaaccaacg cgctggccgt
     2761 cgccaacaag caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac
     2821 ctccgcgcca gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2881 cgatccgatc gtctggcgca agaatctctc cgaaaccacc aaagacaaga tctacgactt
     2941 ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg ctggaacgcc tgggctgggc
     3001 cccgttccgc gcctccagcg acctgcaact ggtgccgatt cgccagctcg cactgtttaa
     3061 agagatgcag ggcgtaaaaa gcaataaagg actgaatgag caggacaagc tggcgaaaac
     3121 caccgagatt caggcgcagc tggatgacct ggaccgcctg aacaacgcgt taagcgcgat
     3181 gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg gtcccctctc ccttgaggga
     3241 gagggttagg gtgaggggga aatgcgcacg ctgatgccct catcccggcc ctctcccaca
     3301 gggagaggga gaaaacatta aacggagcca aacatgcaaa ccatcaccat cgccccaccc
     3361 aagcgtagct ggttctcgct tctgagctgg gccgttgttc tcgccgtgct ggtcgtctcg
     3421 tggcagggcg cggaaatggc cccgcttacg ctgattaaag acggcggcaa catggcaacc
     3481 ttcgttgccg acttcttccc gcccgatttc agccagtggc aggattacct caccgaaatg
     3541 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cagtggttct ctccatcccc
     3601 ttcggtctga tgagcgccga aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc
     3661 ctgatggatg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3721 gtcggtctcg gcccgttcgc tggcgtgctg gcgctattta tccacaccac cggcgtgctc
     3781 tccaagctgc tttccgaagc ggtggaagcg atcgaacccg gcccggtaga aggcattcgc
     3841 gccaccggcg ccaacaagct ggaagagatc ctttacggcg tgctgccaca ggtaatgccg
     3901 ctgctgatct cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3961 ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg aagcgattcg cggttttcag
     4021 ttccaacaaa cctgcgccct gatggtgctt atcatcgtca cggtcagcct gctggatttc
     4081 ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgata tctatgcact
     4141 tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca aaacttgagc
     4201 aggagcttcg tcaacactat cgctgcggcg actatcttcc cgccgagcag cagctggcgg
     4261 cgcgctttga ggtaaatcgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     4321 gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc
     4381 cgctcaacgc ccaggcgcgt tttagccaga acctgctgga tcagggcagc catcccacca
     4441 gcgaaaaact gctgtcggtg ctgcgcccgg catctggtca cgtcgctgac gcgctgggga
     4501 taaccgaggg ggagaacgtc atccacctgc gcaccctgcg ccgggtcaac ggcatcgcgc
     4561 tctgtttaat cgaccactac tttgcggacc tcaccctttg gccgacgctg caacgcttcg
     4621 acagcggctc gctgcacgat tttctgcgcg agcaaa
//
LOCUS       DAJTMK010000247         4623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTMK010000247 DAJTMK010000000
VERSION     DAJTMK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.254"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..678
                     /locus_tag="OO135_003909"
     CDS             <1..678
                     /locus_tag="OO135_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5399551.1"
                     /translation="DAKISQFDLNAIEAASQLATDDDEIAALTVGGSLLQNSKVRKDV
                     LSRGPHSLYLVQDAQLEHALPLDTAKALAAAIEKIGFDLLIFGEGSGDLYAQQVGLLV
                     GEILQLPVINAVSAIQRQGNTLVIERTLEDDVEVIELSVPAVLCVTSDINVPRIPSMK
                     AILGAGKKPVNQWQASDIDWSQSAPLAELVGIRVPPQTERKHIIIDNDSPEAIAELAE
                     HLKKALN"
     gene            698..1636
                     /locus_tag="OO135_003910"
     CDS             698..1636
                     /locus_tag="OO135_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5399552.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1692..2981
                     /locus_tag="OO135_003911"
     CDS             1692..2981
                     /locus_tag="OO135_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5399553.1"
                     /translation="MSDDKFDAIVVGADVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKIFFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGRFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            2978..3271
                     /locus_tag="OO135_003912"
     CDS             2978..3271
                     /locus_tag="OO135_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5399554.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            3328..>4623
                     /gene="fadK"
                     /locus_tag="OO135_003913"
     CDS             3328..>4623
                     /gene="fadK"
                     /locus_tag="OO135_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5399555.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVRAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGR"
BASE COUNT         1103 a         1179 c         1266 g         1075 t
ORIGIN      
        1 gacgccaaaa tcagccagtt cgatctcaat gccattgaag ctgcaagcca gctcgcaaca
       61 gatgacgatg agatagccgc gctgaccgtt ggcggctcat tgttgcagaa ctcgaaagtg
      121 cgcaaagatg tgctatcccg cgggccgcac agcctgtatt tggtgcagga tgcgcaactt
      181 gaacatgcac tgcctctcga taccgcaaaa gcgctggcgg cagcaattga aaagatcggc
      241 ttcgatttac tgatctttgg tgaaggttcc ggcgaccttt atgcccagca ggttggcttg
      301 ctggtcggag aaattctgca acttccggtg attaatgcag tgagtgctat tcagcgtcag
      361 ggcaatacac tggtgattga acgcacgctt gaagatgatg ttgaagttat tgaactctct
      421 gttccagccg tgctctgcgt cacctccgat attaacgtgc cacgcattcc ttcgatgaaa
      481 gccattctcg gcgcgggtaa aaaaccggta aatcagtggc aggcaagtga tattgactgg
      541 agccagagcg cgccacttgc tgaactggtc ggcattcgcg taccgccgca aacagaacgt
      601 aagcacatca ttatcgataa cgattcgccg gaggccattg ccgagctggc ggaacatctg
      661 aagaaagccc tgaactgaag cctacggaga agaaacgatg agtcaattaa acagcgtctg
      721 ggtctttagc gataatcctg aacgttatgc tgaactgttt ggcggcgctc agcaatgggg
      781 ccaacaggtg tatgccattg tacaaaatac cgaccaggcg caggcagtta tgccttatgg
      841 tccaaaatgt atttatgttc ttgagcaaaa cgacgcgctg caacgcactg aaaattacgc
      901 cgaaagcatt gctgccccgc tgaaagataa acaccccgct atgttgctgt tggccgcgac
      961 gaaacgtggt aaagctctgg cagcacggtt aagtgtgcaa ctgaatgcgg cgctggtgaa
     1021 cgatgccacg gcggtggata ttgtcgatgg tcacatttgc gccgaacacc ggatgtatgg
     1081 cgggttagcg ttcgctcagg aaaagatcaa cagcccgctg gcgatcatta cccttgcacc
     1141 cggtgttcag gaaccgtgca ccagtgatac ctctcatcag tgcccgacag aaacggtacc
     1201 ttatgttgct ccgcgtcatg aaattctctg tcgcgaacgc cgtgcgaaag ccgcaagcag
     1261 cgtggacctg agcaaagcaa aacgtgtggt tggcgtcggt cgtggtctgg cggcgcagga
     1321 tgacctgaaa atggtccacg aactggcggc ggtgctgaat gctgaagtcg gctgttcacg
     1381 tccaattgcc gaaggcgaga actggatgga gcgtgaacgt tatatcggtg tctccggcgt
     1441 gttgctgaaa tccgatctct acctgacgct ggggatctcc gggcagatcc agcatatggt
     1501 tggcggcaac ggcgcaaaag tgattgtcgc catcaataaa gataaaaatg cgccaatctt
     1561 caattatgcc gactacggtc tggtgggcga tatctacaaa gtcgtccctg ccctgattag
     1621 ccagttgagc cgctaattcc cctttcaacc acatctcccg ctgtaaacgc agcgggaagg
     1681 gagcataacg aatgtcggat gacaaatttg atgccattgt ggtcggtgcg gacgttgctg
     1741 gtagcgttgc cgcactggtc atggcgcgag ccggactgga tgtcctggtg atagaacgcg
     1801 gcgacagtgc cggatgtaaa aacatgaccg gcgggcgtct ttatgcccac acacttgaag
     1861 caatcattcc aggctttgca gtatcagcgc cggtagaacg caaggtcaca cgcgagaaaa
     1921 tcttcttctt aaccgaagaa agcgccgtta ccctcgattt tcaccgcgag caaccagatg
     1981 ttccgcaaca cgcatcttat accgtattgc gtaatcgtct ggacccgtgg ttgatggaac
     2041 aagccgagca ggcgggcgca cagtttatcc cgggcgttcg cgtcgatgcg ttggttcgtg
     2101 aaggaaacaa ggtcactggc gtgcaggccg gggatgatat tctccaagcg aatgtggtgg
     2161 ttctagctga tggcgtaaac tcgatgcttg gccgctcgct gggaatggtt cccgcttccg
     2221 atccgcatca ttacgctgtt ggtgttaaag aggttattgg cctcacacca gaacagatca
     2281 acgatcgctt taatattacg ggcgaggaag gtgccgcctg gctgtttgcc ggttcccctt
     2341 ctgacggcct gatgggcggg agatttctct ataccaacaa ggattccata tccttggggc
     2401 tggtttgtgg attgggtgat atcgcccatg cgcaaaaaag cgtgccgcaa atgctggaag
     2461 attttaaaca acaccccgcc attcgcccac taattagcgg cggcaaactg cttgaatatt
     2521 ccgcgcatat ggtgccagaa ggcggtctgg caatggtgcc gcagatggtt aacgatggcg
     2581 tgatgatcgt tggtgacgcc gcaggcttct gcctgaattt gggttttaca gttcgtggca
     2641 tggatttagc cattgcatcg gctcaggctg ccgccacaac ggtgatcgcc gccaaagaac
     2701 gcgaggattt ctccgccagc agtctggcgc aatacaaacg tgagctggaa caaagctgcg
     2761 tcatgcgcga tatgcagcat ttccgcaaga tcccggcgct gatggaaaac ccgcgcctgt
     2821 ttagccaata cccacgaatg gtcgccgaca tcatgaacga gatgttcacc attgacggca
     2881 aaccaaacca gccggtacgc aaaatgatca tgggacacgc gaagaaaatt gggctgatca
     2941 acttgctgaa agatggcatt aagggagcaa ccgcgctatg agccagaacg ctacggttaa
     3001 cgtcgacatc aaattaggcg tcaataaatt ccatgttgat gagggccacc cgcatatcat
     3061 tctggcggca aatcccgata tcaatgaatt ccgtaaatta atgaaagctt gccctgccgg
     3121 actttataag caggatgacg ccggcaacat tcatttcgat tccgccggtt gtctggagtg
     3181 cggcacctgt cgggtgctgt gcggcaacac tattctcgaa cagtggcaat atcccgcagg
     3241 caccttcggt attgattttc gctacggcta atcatgcatc ccacaggccc gcatctcggg
     3301 cctgatgttc tgtttcgaga gtccaaaatg aaagtgacat taacgttcaa cgaacaacgt
     3361 cgtgcggcgt atcgtcagca agggttatgg ggcgatgctt cactggccga ttactggcag
     3421 cagaccgctc gtgcgatgcc agacaaaatt gccgtggtcg acaatcatgg tgcaacgtac
     3481 acctatagcg cgctcgatca cgccgcgagc tgtctggcaa actggatgtt agcgaagggt
     3541 attgaatcag gcgatcgcat cgcatttcaa ctgcctggct ggtgtgaatt taccgttatc
     3601 tatcttgcct gcctgaaaat cggtgcggtt tccgtgccgc tgttgccttc ctggcgggaa
     3661 gcagaactgg tgtcggtgct caataagtgt caggcaaaaa tgttctttgc accgacgttg
     3721 tttaaacaaa cacgtccggt agatttaatc ctgccgctgc aaaatcagct tccacaacta
     3781 caacaaattg tcggcgtgga caaactggct cccgccacct cttccctctc attaagtccg
     3841 attatcgccg acaatacctc actgaccacg gcgataacga cccacggcga tgaattagct
     3901 gcggtgctgt ttacctccgg aactgagggt ctgccaaagg gcgtgatgct aacgcataac
     3961 aatattctcg ccagtgagcg ggcttattgc gcgcgactga atctgacctg gcaggatgtc
     4021 tttatgatgc ctgcgccact tggtcacgca acgggctttc tgcatggcgt aagggcacca
     4081 ttcttaattg gcgctcgcag cgtgttgtta gatattttca ctcctgatgc gtgtctcgcg
     4141 ctgcttgagc agcagcgttg cacctgtatg ctaggcgcaa cgccgtttgt ctatgatctt
     4201 ttgaatgtac tagagaaaca acccgcggac ctttcagcgc tgcgtttctt tctttgcggc
     4261 ggtaccacaa tccccaaaaa agtggcacgt gaatgccagc agcgcggcat taaattatta
     4321 agtgtttatg gttccacaga aagttcgccg catgcggtgg tgaatctcga tgatcctttg
     4381 tcgcgcttta tgcacaccga tggttacgct gccgcaggtg tagagattaa agtggtcgat
     4441 gacgcacgca agaccttacc gccaggttgc gaaggtgaag aagcctcgcg tggccccaat
     4501 gtgtttatgg ggtattttga tgaacctgaa ttaaccgccc gtgcgctgga cgaagaaggc
     4561 tggtattgca gcggcgatct ctgccgtatg gatgaggctg gctatataaa aattaccgga
     4621 cgc
//
LOCUS       DAJTMK010000248         4577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTMK010000248 DAJTMK010000000
VERSION     DAJTMK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.255"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..323)
                     /locus_tag="OO135_003914"
                     /pseudo
     CDS             complement(1..323)
                     /locus_tag="OO135_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(316..3576)
                     /locus_tag="OO135_003915"
     CDS             complement(316..3576)
                     /locus_tag="OO135_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5399556.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWGSVKA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPVLISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAALYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWMTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            3621..3950
                     /locus_tag="OO135_003916"
     CDS             3621..3950
                     /locus_tag="OO135_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5399557.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3937..4329
                     /gene="tnpB"
                     /locus_tag="OO135_003917"
     CDS             3937..4329
                     /gene="tnpB"
                     /locus_tag="OO135_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5399558.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKARSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            4336..>4577
                     /locus_tag="OO135_003918"
     CDS             4336..>4577
                     /locus_tag="OO135_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5399559.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAG"
BASE COUNT          966 a         1466 c         1075 g         1070 t
ORIGIN      
        1 aatcgtacaa ctgaaatcac tgtacctggc gttatctgtg acgctccact cccggcatac
       61 caccctcacc gtccggttat gtttcggcgg tcgccacaaa aaggcacggt aaccaccatg
      121 ccacgataaa aactcttcca gccatcgccg ggtcgcctca tccgtcaccc ggaacaccgc
      181 ctgaaacgtc ttcagtcggg cattaagtcc cgtcgggcgg cgctgttcat aaccgtcacc
      241 aaaccgtacc ctcgccaccg acggtttctc accacctgca tcccttcacg cgggaccaga
      301 tgcagcgttt ttatctcagc cactcagcat tcctccgtca cgtcgcatgg acagcatcac
      361 cgcctgcacc cgctggtcaa tcagttgcac aaggctgccc gcagcttccg gccctatctg
      421 gccattagtc ccgtcattct gaatggcgat atggtagacc ggggaataca ccagacctgc
      481 actgccgttc atactgccca ccgctcgcac acccagcgag ccatccgccg cccgggtcag
      541 gggcataatg gcttcaggac cggcctcccc catcagtccc gccccttttg caaaggcaaa
      601 gtacgtgggc gtatccacaa tactgttgct gtacgcactc aggtttgccg aggtatacac
      661 accgcctttt gcattggcca ccgcaccgcc cagccagcta ccaatactgc cgataaatcc
      721 tcccgctccg gacatactgt ttgccgccgt cttgatcccg ttgacaatcg cggcattcat
      781 aagaactttt gatatttcct gcagcactga tgaggcccag ctgcgccatt ccactttatt
      841 tccgttcagc atctccgtga tgttattcac catccctgag ataccctccg tcgcaagctg
      901 tgctgcctgt gaggcgtaat cggacgcatt atccacccag ttactgaatc cctcctgcag
      961 ccctttctgc cagtccgcac gctgcacatc cgattcggca taaaaggctt cctgctcttt
     1021 cagacgttca ctcagatact gcgcgttctg tgccagagcc tgtctgtaaa aatcctcact
     1081 gatatccccg gtctgatact gagactgaag gtccgcatcc ttctggcgga aactgtcgcg
     1141 gatctgctgc aactcccgca tgcgttcacg gagccgctct ccctgcccgt accccagcag
     1201 ttccgcttca tttgatgcac gcgcagccac attatcattc ttcagggtct cttcccggga
     1261 ccgcaactgc tcccggattt tttgctggtc aatcagggcc gcattgcgca gcagttcctg
     1321 cttctgcatc tccgtcaggg ttttcagttc gccctgcgca gtctggtatt tcagcttcgc
     1381 cagctctgta ttctgccccg ccagtgccag ttgctctttc tgctgcttca gcagccggga
     1441 aaaactgtct tccgcttttt ccgtctctga ttttccaccc cgggatttgg gtttattcgc
     1501 ctcgttattg cgccaggctt ccagggcatt actgatataa cgttgtctcg cctcctgata
     1561 cggatcaccc acaaaaccga ggtcatccgc cgcatacccc agtcggacac gctctttttc
     1621 ttcccctttc agtctggaca gggccagctc acgctctgtt tttgtcaggg cactctgctg
     1681 tttatcatcc agagtggcct gtggcagccg taacggcaca ttcaccagtc cctgccgctg
     1741 ctgaagcagt tcattaccca gccccagcag acggttgaat tccgtatgct gaccgttcat
     1801 aaccagcatg gactggtaca ccttattctg ctctgccgcc tgctgacgaa ttaacgccac
     1861 acgacggtct tccagcccgg caagcacatc ctgaatggac tgcgcttttt cctgcatcag
     1921 tgccagacgg gactgctcaa cggcaagctg ctctgttgcc tgagcaagcc cttccgttac
     1981 ggtcttcacc gaggtcagat ggtttatcat gaatccgtca ccggtcatcc agcccgggtt
     2041 cgccagaaca tactgatatc ctgcgatttt ttcctgcagg gatttcaccc gactagcctg
     2101 ttcatcaatc agccggttct gctctgtcag cgccgcccgt gttcgtcctt cattatctga
     2161 ggcttcaggc aaagacattg acggcgtttt atgcgcgatt tcatctatcg tcagtgcata
     2221 ctggcgcgca gactcccagg cctgctcctg attctggtac agcgtgtaca atgctgcagc
     2281 ccccagcatc accagtccgg gtacgccacc aaccagcccc agcgcaccgc tcatcagacg
     2341 tgagcccacc gccgttgtac tgttcagcgc attctgggcg gcgcttctgg cagcaatatt
     2401 tctgttcagg cgttcctgtg tggccgccag acgggcctct gcagcaatct gcatctccgt
     2461 cccgcgggct gccgccacgg cctgctgagc acggtacacg gctgcccttg cccgcgccgt
     2521 ggcaatctgc gttcccctga actgtgcttc cgccagtgca acttcattac gtgcagccgt
     2581 cacaagtcct gccgtggcag acatcgctcc ggaggccata ttgccaaagt accgggcaac
     2641 cccgacggca accagcgccc ccacagctgt tgccacatta tcaatctgtc cggcaacacc
     2701 gttcagcacg ccggagagcg tttttgtcac cccgcaggcc tcattcactt cgcccaccca
     2761 ggccataaag gcgttttcca ccttcgtgat actaccggaa accgtttccg gcatggccgc
     2821 atattcatca cgtaatatcc ccagctggct gattaacaca gggaccactt tatccgctgt
     2881 cagttttccg tcatccgcca ttgccttcag atctttacgg gccacaccca tccccgcagc
     2941 aagcgcgcgg atcacccggt cgccattttc gttaacagca ttaaattctt cgccacgcag
     3001 tacaccctgt gccagtgcct ggctgaactg ggtgatcacc gaaccggatt ccgcaactcc
     3061 agcccctgac agtttcagtc ctgtcgaaat ggcctccgtc accttcagca catcatcagc
     3121 actgtaacca tattcacgca tcgaggcagc cgaacgggca aacagggccg cattatccga
     3181 aaacgcggta cctgttcgct gactgatatc catcagcact ttctgtgatg acgagaattc
     3241 atccgatgac tgagatgcct gtttcagacg ggccttcacg gaaccccact catcggccag
     3301 cgaaatcagg tgtccggtgg caaaggcacc ggcaaatgcg ccagccattc cgacagcaga
     3361 accgcgaatt tccgtcaact gactgttcag ttctgcaagg gcgcggcgct gttcccgtgc
     3421 ggcagcagcg gcatgacgtc cgccattctg cagggtccgg taatattcac tgcccatacg
     3481 ggacgcccgc tggatctccg actggaatga ctgtgaattt gccgaaattt tgagtgtcgt
     3541 tacggggaag tcatcttcac agggatcttc ggtcagttag tattaccgac atccattcac
     3601 caacttaccg gagataatcg atgtcaaaac cccgatggac cattgaacag aaaaggcagc
     3661 acgttgctgc ctggcgcgcc agtggtctta cacgtcagca atactgtgaa ctcaacgata
     3721 ttcccttcac atcacttcgc gaatggccgc aggatgtcgc aaaagctgaa cgccgggcaa
     3781 atgaaccgac cgttcttccc gttcatattg cgccaccact gcatgctgat gtaccacagc
     3841 ccgtcacgaa tgagccagtc acgctcttcc ttcccggtgg agtacggatg tgctgtcagc
     3901 cgtcacaact taccgatgtc ttcagggcac tcaaatatgc tcagtcctga taatgtcttc
     3961 atcgcaatta aacctgttga catgcgccgg ggcatcgact cactgacgca gtatatacag
     4021 gatgaactcc ggtcgacatg gcatgaggga gccgccttcg tctttgttaa caaagcccgt
     4081 tcgcgtatca aagttctccg gtgggataaa cacggggtgt ggttgtgtac ccgccgtctg
     4141 cacaaaggca gcttccgctg gccacgtgca aatgacgctg cctggcacct cactcccgac
     4201 gagtttaact ggctgattgc cggtgttgac tggcagcagg ttaagggaca tgacctgacg
     4261 aaatgggtct ggcagaatga acctgaactg cgccctgaaa acacgcaaaa tacactgcta
     4321 actcagtgaa aaataatgaa tatccgtatc tggagtggta tactcccctg tatggatatc
     4381 tccgctctca acaccacgaa tgacatcgaa aaactgcgtg ctatggcact tgccatggta
     4441 caagaagtca tgtcggagaa tgccgaaaaa gagcgggaat tactggagaa aagccggcgc
     4501 atccagcttc tggaagaaat gctgaaactg gttcgtcaac agcgcttcgg aaaaaaatgt
     4561 gaaacgctgg ctggtat
//
LOCUS       DAJTMK010000249         4577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTMK010000249 DAJTMK010000000
VERSION     DAJTMK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.256"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>4577
                     /locus_tag="OO135_003919"
     CDS             <1..>4577
                     /locus_tag="OO135_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5399560.1"
                     /translation="AGNPVIGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSG
                     TLTLMPQLNGVDAAKAPAVVNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDEND
                     KPVKEQKQQLNTAVSIDNVKPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWN
                     EDLHTAGFIIDANPQSAKIATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTF
                     AVLSGSATSFNNQNTAKTDVNGLAAIDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDS
                     STAQVDLQKSKNEVVADGNDSATMTATVRDAKGNLLNDVKVTFNVNSAAAKLSQTEVN
                     SHDGIATATLTSLKNGDYTVTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTN
                     TAPQHMTATLQDKNGNPLKDKEITFSVPNDVASRFSISNGGKGMTDSSGVAIASLTGT
                     LAGTHMITARLANSNVSDAQPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKD
                     PFDNVVKNLSVVFRTSPADTQLSLNARNTNENGIAEVTLKGTVLGVHTAEAILLNGNR
                     DTKIVNIAPDASNAQVTLNIPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTIPQ
                     DVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKD
                     SAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNAVKDLQVTFSTNPADTQLSQSKSNT
                     NDSGVAEVTFKGTVLGVHTAEATLPNGNNDTKIVNIAPDASNAQVTLNIPAQQVVTNN
                     SDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGK
                     KAGTHTVTATLSNNNTSDSQPVTFVADKTSALVVLQISKNEITGNGVDSATLTATVKD
                     QFDNEVNNLPVTFSTASSGLTLTPGESNTNESGIAQATLAGVAFGEQTVTASLANTGA
                     SDNKTVHFIGDTTAAKIIELTPDPGSIIAGTPQNSTGSVITATVIDNNGFPVKGVTVN
                     FTSNAATAEMTNGGQAVTNEQGKATVTYTNTRSSIESGARPDTVEASLGNGSSTLSTS
                     INVNADASTAHLTLLQALLDTVSAGDTTNLYIEVKDNYGNGVPQQEVTLSVSPSEGVT
                     PSNNAIYTTNHDGNFYASFTATKAGVYQVTATLENGDSIQQTVTYVPNVANAEITLAA
                     SKDPVIADNNDLTTLTATVADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAVTDTEG
                     KAKVTLKGTKAGAHTVTASMAGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMT
                     RLQATVTDGNGNPLANEAETFTLPADVSASFTLGQGGSAITDINGKAEVTLSGTKSGS
                     YPVTVSVNNYGVSDTKQVTLIADAGTAKLASLTSVYSFVVSTTEGATMTASVTDANGN
                     PVEGIKVSFRGTSVTLSSTSVETDDRGFAEILVTSTEVGLKTVSASLADKPTEVISRL
                     LNAKADINSATITSLEIPEGQVMVAQDVAVK"
BASE COUNT         1340 a         1145 c         1155 g          937 t
ORIGIN      
        1 cagcaggtaa tcctgttatc gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca
       61 tcaccctttc tgactggaaa gataatggtg acggaagcta tacccagatc ctgaccacag
      121 gtgcgatgtc tggcacgctg acgcttatgc cacagctgaa cggtgtggat gcggctaaag
      181 cccccgccgt ggtgaatatc atttctgttt cgtcatcccg gactcactcg tcaattaaga
      241 ttgataagga ccgttatctc tccggcaatc ctatcgaggt aacggtagaa ctgagagatg
      301 aaaatgacaa acctgttaag gagcaaaaac agcaactgaa taccgcagtc agcatcgaca
      361 atgtgaaacc tggtgtcact acagactgga aagaaaccgc agatggcgtc tataaggcaa
      421 cctataccgc ctataccaaa ggcagtgggc ttactgcgaa gctattaatg caaaactgga
      481 atgaagattt gcataccgct ggatttatca tcgacgccaa cccgcagtca gcgaaaattg
      541 cgacattatc tgccagcaat aatggtgtgc tcgccaatga gaatgcagca aacaccgtct
      601 cggtcaatgt cgctgatgaa ggaagcaacc caatcaatga tcataccgtc acgtttgcgg
      661 tattaagcgg atcggcaact tcctttaaca atcaaaacac cgcaaaaacg gatgttaatg
      721 gtctggcggc tattgatctg aaaagtagta agcaggaaga caacacggtt gaagtcaccc
      781 ttgaaaatgg cgtgaaacaa acgttaatcg tcagttttgt cggcgactca agtaccgcac
      841 aggttgatct gcagaagtcg aaaaatgaag tggtcgctga cggcaatgac agcgccacaa
      901 tgaccgcgac agttcgggat gcaaaaggca acctgctcaa tgacgtcaag gtcaccttca
      961 atgttaattc agcagcagcg aaactgagcc aaaccgaagt gaatagccac gacgggatcg
     1021 ccacagctac gctgaccagt ttgaaaaatg gtgattatac ggttacggcc tctgtgagct
     1081 ctggttctca ggctaatcaa caggtgaatt ttatcggtga tcaaagtact gcagccctga
     1141 ccctcagtgt gccttcaggt gatatcaccg tcaccaacac agctccgcaa catatgactg
     1201 caaccttgca ggataaaaat ggcaacccac taaaagataa agaaatcacc ttctctgtgc
     1261 caaacgacgt cgcaagtcgg ttctcgatta gcaacggcgg aaaaggcatg acggatagta
     1321 gcggggttgc aatcgcctcc ctgaccggca cgttagcggg cacgcatatg atcacggctc
     1381 gtctggctaa cagcaatgtc agcgatgcac agccaatgac gtttgtggcg gataaagaca
     1441 gagcggttgt cgttctacaa acatcgaaag cggaaatcat tgggaatggc gtggatgaga
     1501 cgaccctgac agcaacagtg aaagatcctt ttgataacgt ggttaaaaat ctgtcagtag
     1561 tcttccgcac ctcccccgca gacacgcaac tgagtctgaa cgcgcgtaat actaatgaga
     1621 acggtattgc tgaagttacc cttaagggca cggttttggg tgttcataca gccgaagcca
     1681 tactgcttaa cggcaacaga gatacgaaga tagtcaatat tgcgcccgat gccagcaacg
     1741 cgcaggtcac cctgaacatc cctgcacaac aggtggtgac gaataacagc gacagcgtgc
     1801 agctgacggc gacggtgaaa gatccgtcga atcatccggt ggcgggaata acggtgaact
     1861 tcaccatacc acaggacgtg gcggcaaact ttacccttga aaataacggt attgccatca
     1921 ctcaggccaa tggcgaagcg catgtcaccc tcaaaggcaa aaaagcgggc acgcatacgg
     1981 ttaccgcaac gctgggtaac aataatgcca gcgatgcgca accagtcacc ttcgtggcgg
     2041 ataaggacag cgcggttgtc gttctgcaaa catcgaaagc ggaaatcatt gggaatggcg
     2101 tggatgagac gactctgacg gcaacagtga aagatccttt tgataacgca gtaaaagatc
     2161 tacaggtcac cttcagtacc aaccccgcag atactcaact tagtcagagc aaaagcaata
     2221 ctaacgacag tggtgtggcc gaagttacct ttaagggcac ggttttgggt gttcatacag
     2281 ccgaagccac actgcctaac ggcaacaacg atacgaagat agtcaatatt gcgcccgatg
     2341 ccagcaacgc gcaggttacg ctgaacatcc ctgctcaaca ggtggtgacg aataacagcg
     2401 acagcgtgca gctgacggcg acggtgaaag atccgtcgaa tcatccggtg gcgggaataa
     2461 cggtgaattt caccatgcca caggacgtgg cggcaaactt taccctcgaa aataacggta
     2521 ttgccatcac ccaggccaat ggggaagcgc atgtcacgct caaaggtaaa aaagcgggta
     2581 cgcatacggt taccgcaacg ctgagtaata acaataccag tgattcacag ccggtaacgt
     2641 ttgtggcgga caaaacctcg gctctggttg ttcttcagat atcaaaaaat gagatcacag
     2701 gtaatggcgt agatagcgca acgctaactg caacggtcaa agatcagttc gacaatgagg
     2761 tgaacaatct tccggtaaca ttcagcacag cttcttcagg cctcaccctg accccagggg
     2821 aaagtaatac caatgagtct ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg
     2881 agcagacggt tactgcatca ctggctaata ctggtgccag cgacaacaaa actgtgcact
     2941 ttattggcga cacaacggcg gcaaaaatta tcgagttgac gcctgaccca ggcagcataa
     3001 tcgccggtac cccacagaac agcaccggta gcgtaatcac cgctacagtc attgataata
     3061 atggctttcc ggtgaaaggt gtgactgtga acttcaccag caacgcagcg acagccgaaa
     3121 tgacgaatgg cggccaagcc gtaacgaacg aacagggtaa ggctaccgtc acttatacca
     3181 atacccgctc ctcgatagaa tcaggagcga gaccggatac cgttgaggcc agtctgggaa
     3241 atggtagctc tacgcttagc acatcaatta atgtcaacgc tgatgcgtct acggcacatc
     3301 tcaccttgtt acaggcactt cttgatacag tctccgcagg cgacactacc aatctgtata
     3361 ttgaggtgaa ggataattac ggcaacggtg taccccagca ggaggtaacc ctcagcgttt
     3421 caccaagtga aggcgtgact cccagtaata acgctatata tacgaccaat cacgacggca
     3481 atttttacgc aagctttacc gctacaaaag ccggggttta tcaagtgacg gcaaccctcg
     3541 aaaatggcga ttcgatacaa caaacggtga cctatgtgcc gaacgtcgcg aatgccgaaa
     3601 tcacgctggc agcctcgaag gatccggtga ttgccgacaa taacgatctc acgacactaa
     3661 cagcaacagt cgctgataca gagggcaatg cgatagccaa cactgaagta acatttactc
     3721 tgccggaaga tgtgaaggcg aacttcacgc tgagcgatgg cggtaaagcg gttactgata
     3781 ctgaaggcaa agcgaaagtc acgctgaaag gtacaaaagc aggcgctcat actgttacag
     3841 catcgatggc tggcggtaag agtgagcagt tggtggtgaa ttttattgcg gatacgctca
     3901 cagcgcaggt taatcttaac gttaccgagg acaactttat cgctaataac gtcgggatga
     3961 ccaggctgca ggcaacagtg actgatggaa acggcaaccc gttagccaat gaggcggaga
     4021 cattcacgct accggcagat gtgagcgcaa gctttactct cggacaaggc ggttccgcca
     4081 ttactgacat caacggcaag gctgaagtta cactgagcgg tacaaaatcc ggctcctacc
     4141 ccgtgacagt tagcgtgaac aattatggtg tcagtgatac gaaacaggtg actttgattg
     4201 ccgatgctgg taccgcaaaa ctagcctcct taacctctgt atactcattc gtcgtcagca
     4261 cgaccgaggg cgcgaccatg actgcaagcg tcactgacgc taacggcaac ccggtagaag
     4321 gcataaaagt tagtttccgc ggaacctccg tcacgctaag cagcaccagc gttgaaacgg
     4381 atgatcgggg tttcgctgaa attcttgtga caagcaccga ggtcggactg aaaacagttt
     4441 cagcctctct ggcagataaa cctactgaag tcatctcgcg attactgaat gcaaaagcag
     4501 atattaattc tgcaacgatt accagtctgg agatacctga aggtcaggtc atggtcgcac
     4561 aagacgtagc agttaaa
//
LOCUS       DAJTMK010000250         4530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTMK010000250 DAJTMK010000000
VERSION     DAJTMK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.257"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            291..663
                     /locus_tag="OO135_003920"
                     /pseudo
     CDS             291..663
                     /locus_tag="OO135_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1225..2172)
                     /gene="icsP"
                     /locus_tag="OO135_003921"
     CDS             complement(1225..2172)
                     /gene="icsP"
                     /locus_tag="OO135_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5399561.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSAWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3553..3705)
                     /locus_tag="OO135_003922"
                     /pseudo
     CDS             complement(3553..3705)
                     /locus_tag="OO135_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3761..4453
                     /gene="ospZ"
                     /locus_tag="OO135_003923"
     CDS             3761..4453
                     /gene="ospZ"
                     /locus_tag="OO135_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5399562.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCSEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENRDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RLSQIKAQISVT"
BASE COUNT         1395 a          885 c          883 g         1367 t
ORIGIN      
        1 gcagcgggct tgtaatgggt taagtgataa cagatgtctg gaaatatagg ggcaaatcca
       61 gctgcgatct ggttcaacaa tatgcagatg gtacacaacc tctttaacca gcccatccgg
      121 attattcaga gccgcttccg caatccggaa taaaagtgct gttttaccgt gaaccctctg
      181 aaaatcagcg agcagtgttt taaccagccg acgttcagag cgtactgaca gacggtggac
      241 aatttgaata aacagttcaa tcagaccgta gatgctctcc cgttggcgac atgctgggag
      301 cggtggaaca ccgcttcggc aacgagcttc cggcgtctcc agtgaagtga ctgacggata
      361 atggttcatg ctaccgggct aatgaaacac gccagttcac ccggatgttg ggactttgtt
      421 gaaccgaaga acacggcggt gcggagtccg gagagtaaca gaatagcaaa gagcttcgtg
      481 aaaacaataa agtgtgacta catcagtatc atgcccaaac ctgacgggtt aacggcagca
      541 aagaaccttg cagaggcatt cgagcattat aacgaatggc atccgcatag tgcgctggat
      601 tatcgctcgc cacgggaata tctgcggcag cgggccaatg ataacaggta tctggaaata
      661 taggggcaaa gccacccacg aacgatccag catataatca agaaattaga tttcatttaa
      721 cataagatag attatgtgca caaagaggca tttccttaat ttctgaattt acaggctctt
      781 ttgctctctt tgctatgctg tggatttgga aagatagagt gctggctgat tatttaggta
      841 ttttcatcag catgtccctg atagcactgt tccatcagcg ctttcaggcg ataatagtag
      901 accttgggta gcctctgccc atgtgtaact tgataccccg agaacagcca cgatgatcta
      961 agcggttctt atttctccgg tctcagggtc ggtaatgcca acagtaagtc cacagtaatc
     1021 aacgaagagt tttttgaacc gccccggaaa tcctggagac taaacgccct gagaaagagg
     1081 taaacagaat gactaaaaat acacgttttt cccccgaagt ccgtcaacgg gcagttcgta
     1141 tggttctgga aagtcagggc gaatatgact cacaatgggc ggcaattttt agccatgaaa
     1201 acgtaatcaa ctcggtgaac caattcaaaa aatatacttt atacctgcgg aagtgagaaa
     1261 accaatatat tcaatacttg cactattttt aatggtgcca gatatattgg tactttgctc
     1321 ataaagagat gtatcacctt ttttattaga aatgtaattt ctggagcctt ctatataaaa
     1381 ttttgcactg ggggtgacat agtatccaat attcgcagca acagagcagt aattttggtt
     1441 ttttatttca tctttaaaaa ttgtctcagt ctgatagtgt ttatctgtat cagatgaacg
     1501 aacccaagca ctatatttca gttctgctcc aaactcaaaa ttctcatggc ggtaattagc
     1561 agttaatcca atataaggta ttttgaagag ctgtttgtaa cctattgttc tttcaccact
     1621 aggatgtgcc ccttttttat tcctgttccc cccgttttca ctataaatat aactccctcc
     1681 cattgcatta aaactgtaac gactctcttg gtaacctgct attagtccaa gtcgataatc
     1741 caaattattt aataaccaac ctttaagatt cagctcgaat tcgttagcat cacggagatg
     1801 cgtattggga tgccagctct ggtcggtcca tacctgaggg gtattggaat tgttccagtc
     1861 tttatcaacc atgcttgctt tctggttacc taaagtagtc caaccggaaa ctccgaaaga
     1921 cacttttgga attagcttcc aatcgatgcc acctctaaca atagtcgcat tactgtattt
     1981 ccagtccagt tgactaattt ttctcccccc ttccttggga tgataaacgc gttcttttgt
     2041 tttgccactc agagatccta gactaatatc cgtactgatg ttatccggta tgaaaagtgg
     2101 atagttagtg gtagcatgtg tagtaaagat cgcaggtaca caaagtgcaa gtacaaggaa
     2161 ttttaatttc atcgagaact ctactttttt ggttgaaatg tccatgatct tcttacttta
     2221 taagataaac gtcaatgcca agcattattt taattaaata tttgtttatg ttaccaagaa
     2281 catatctttt attataatat ttattgaact tttgtaaaaa taaaaaaata cctcattgtg
     2341 cgaataaagt aacgggggcc atagatttct ctacctacct tttggtgttc atacctgagg
     2401 ctgatagttt caggattccg cgacaattct actcaccttc ttgttctctc gccaatatga
     2461 aatcaatcag aaaagcgttc cataagaaca acgataagac aataaattgt catcacggct
     2521 gcaatagcca acgtgccttt cagtaccaac atgacaacct cctgtatttg acgtaacgtg
     2581 tgctgataaa tatgtatttg tatctcaacc gactcaatac aacacttaag tatagttttt
     2641 cagattttgt ttttcccgtg aaatattatg gaaatggcag aagcaataca ttgaattccg
     2701 attcgagtga atacggctag cgggcacctc actttagcac tgaagccatc aacttttatt
     2761 tgacttattt caatggaaag ctttctcact atcagtacag gtaattccca tccttttaaa
     2821 gtcgagagtc agactgcgac taatacagtt aaagtgacca aactcagtta tcctctacac
     2881 attttacctc atgtttactt ggacaattca ttggtgcttt tataaaagaa taaatattct
     2941 actaatttaa tgcatcacct aaaatagtat tttttataat agaactgtat ctatagtata
     3001 ttattaaagc ttaattattt attatgcttt aatgtggttg tccgattaag gactaatcct
     3061 tgaaaacgcc gtatagggcg ggagttcact ctattgcgat aacgtaaccg tacaatattg
     3121 tgcgtttccc aaattgacca ctactgaact attctgggat ttaaatgcaa taaaatggtt
     3181 ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc cagatattac
     3241 ttataataaa gaggcttggc agtttggata agctattgtc cttattccac taatacaaac
     3301 tttttgtaaa atatattaaa tatacttcat ttcatactga aatccccgga agtattcaat
     3361 caaacaatta caccataaaa ggaagaggga gccccaatta agttatcaag catgtttatg
     3421 atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg tctttattga
     3481 tcctacccac ataatatgga cgcagtcgta agtgaggccc tgtgagccta cccacgtaat
     3541 atggacacag gcctaagcga ggttctggtt ttcaaattgt tccggactga ggccgccaca
     3601 ccaacggtgc cgtcgccagc gattgtaatc gcactcgata taattaaaca ctgttgcccg
     3661 cactatttcc cgatagataa agtgttctcc gtggagacat tccacttagg aacaattata
     3721 ttgttcatgg taatgtttat atttgagtat agagattaaa atgattagtc ccatcaagaa
     3781 tattaagaac gtatttccaa taaatactgc aaatactgaa tatatagtga gaaatattta
     3841 tcctagagtt gaacatggtt actttaatga atcaccaaac atatatgaca agaagtatat
     3901 ctctggtata accagaagta tggcacaatt aaaaatagag gagtttatta atgagaaatc
     3961 cagacgactt aattatatga agacaatgta ttctccatgt tcggaagatt ttcagcctat
     4021 ttccagagac gaagccagta taccggaagg aagctggttg acagttatat ctggaaaacg
     4081 tcctatgggg caattttctg tggatagttt gtaccatcct gacttacatg cattatgtga
     4141 gctcccagag attagttgta agatcttccc taaagaaaat agggattttt tgtatataat
     4201 tgttgtattc agaaatgatt ctccccaagg agaactacgg gcaaatcggt ttatagaact
     4261 atatgatata aaaagagaga ttatgcaagt gttaagagat gagtcgccag agttaaagtc
     4321 tattaaatct gagattatta ttgctcgtga aatgggagaa ctttttagtt atgcatcaga
     4381 ggaaatagat agctacataa aacaaatgaa tgacaggctt tctcaaatca aggctcaaat
     4441 atctgttact taatgtatat taattgtatg aagtggtcaa caaaaactgg ccaccgagtt
     4501 agagtttttc cagtatcgat tttccgattc
//
LOCUS       DAJTMK010000251         4440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTMK010000251 DAJTMK010000000
VERSION     DAJTMK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.258"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(143..1234)
                     /locus_tag="OO135_003924"
                     /pseudo
     CDS             complement(143..1234)
                     /locus_tag="OO135_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1242..1809
                     /gene="parM/stbA"
                     /locus_tag="OO135_003925"
                     /pseudo
     CDS             1242..1809
                     /gene="parM/stbA"
                     /locus_tag="OO135_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
     gene            1812..2163
                     /locus_tag="OO135_003926"
                     /pseudo
     CDS             1812..2163
                     /locus_tag="OO135_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
     gene            2270..2407
                     /locus_tag="OO135_003927"
     CDS             2270..2407
                     /locus_tag="OO135_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399563.1"
                     /translation="MKLIHGAYEKAAPIIKIGEFAMSGNNVSDGLFASPERDIAASHE
                     R"
     gene            complement(2539..2814)
                     /locus_tag="OO135_003928"
     CDS             complement(2539..2814)
                     /locus_tag="OO135_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5399564.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(2814..3098)
                     /locus_tag="OO135_003929"
     CDS             complement(2814..3098)
                     /locus_tag="OO135_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5399565.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDHPLPVPR"
     gene            3622..3801
                     /locus_tag="OO135_003930"
                     /pseudo
     CDS             3622..3801
                     /locus_tag="OO135_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            3851..4060
                     /locus_tag="OO135_003931"
                     /pseudo
     CDS             3851..4060
                     /locus_tag="OO135_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(4142..4243)
                     /locus_tag="OO135_003932"
                     /pseudo
     CDS             complement(4142..4243)
                     /locus_tag="OO135_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4332..>4440
                     /locus_tag="OO135_003933"
     CDS             4332..>4440
                     /locus_tag="OO135_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399566.1"
                     /translation="MEQKALSAEPRRSFSNEFKLQMVKLASQPGASVARI"
BASE COUNT         1173 a         1068 c         1056 g         1143 t
ORIGIN      
        1 ctgtataaaa accgaccatg cagagagaga aaacaccgga gtggagggaa aagcagaaaa
       61 gctcacgtgg cataaggcgt ggacagcggt atcgtctggt ttttcaattt cctatacgtg
      121 aacacctgac ggcaggtatc actcacccgg tgaaggtaaa tccctcaaga ggcagcaact
      181 ctgccagtcg ctcctccggc cattccggca gacgcgtcag gacgtccgtc agccaggcat
      241 gtggctccag accattacgt tttgctgttt ccagaaggct cattatctgt gccgcccggt
      301 ttccggccat cagtgaaccg gcgaacagcc acgctttccg tcccataaca accgggcgaa
      361 tggcacgctc gcagatattg ttgtccaagg gcaccgctcc gtcacccagg aagcagctca
      421 gttcatcgcg ccggttcaga atatagttaa tcgccttatt taatggcccg tcgggcggac
      481 agcacggctc ctgctcttca agccaggcaa acaggtcatt cactatcggc cgggagtacc
      541 gttatcgcca ctgccgtatt ttttccacag ggcgctcacg gatgagtttc tcaatgcggt
      601 aaagacccgc gatttttctc agctccacgc ctgcacgggg ctccctgctg gccttataca
      661 gatttgcgaa gccgcgccct gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat
      721 tatccgccag agatttgtac acgctgtatc cgtcactcac cagcgtaccg ccacaccagc
      781 actgcagcca ggtctcaggg tatctgaggg cccgtccggt ctgcgagtca aaacagacga
      841 ccggcggacc gcttctttca ccgctgacgt aagcacacag ccagccggag caggatttac
      901 cgcctttccg ggtatcaagg agcctcaggg acgtttcatc tgcatgaata acatcccgtg
      961 tcagaagctc atggcgtagc agtttcgcca gcggggccag tgcagctccg gcaacaccga
     1021 ccatatccgc cattgtactg acggggagct ccacacccat tcgggagaaa atgtgctgct
     1081 ggcgatacag gggcagatgg tcggtatatt tgctgatgac aacctgtgca attaccgacg
     1141 actcaacagc actttcaggg aggatatgcg ccggcatttt cccgctgaac actttctcac
     1201 agcagggaca gctgtactgc ggacggatat agcggattgc attaaatggt ggggatacat
     1261 tcacaataaa agatgtaaaa gtcatgcctg aatctatacc ggcaggttat gaagttctac
     1321 aagaactgga tgagttagat tctttattaa ttatagatct cgggggcacc acattagata
     1381 tttctcaggt aatggggaaa ttatcaggga tcagtaaaat atacggagac tcatctcttg
     1441 gtgtatctct ggtcacatct gcagtaaaag ataccctttc tcttgcgaga acaaaaggaa
     1501 gtagctatct tgctgacgat ataattattc acaaaaaaga taacaactat ctgaagcaac
     1561 gaattaatga tgagaacaaa atatcaatag tcaccgaagc aatgaatgaa gcacttcgta
     1621 aacttgagca acgtgtatta aatacgctca atgaattttc tggttatacg catgttatgg
     1681 ttataggcgg tggcgcagaa ttaatatgcg atacagtaaa aaaacacaca gattcgtaat
     1741 gaacgttttt tcaaaaccaa taactctcaa tatgatttag ttaacggtat gtatctcata
     1801 ggtaattaat aatggacaag cgcagaacca ttgccttcaa actaaatcca gatataaatc
     1861 aaacagataa aattgtttgt gatacactgg acagtatccc gcaaggggaa cgcagccgcc
     1921 ttaaccgggc cgcactgacg gcagatctgg ccttatacag acaagatccc cggcccccca
     1981 tttcttttat gtgaactgct gacgaaagaa accacatttt cggatatcgt gaatatattg
     2041 agatcgctat ttccaaaaga gatggtcgat tttaattctt caacaatcac tcaatcctct
     2101 tcacaaaaag agcaaaaaag tgatgaagag acccaaaaga acgtgatgaa gctaataaac
     2161 taattcaatt attattgagt ttcctttatc cactatcagg ctggataaag gaaacttaat
     2221 caagttattt tcttgccaat cattacataa aataaacagg gtcgttatta tgaaattaat
     2281 ccacggtgct tatgaaaaag cggctccaat aatcaaaatt ggtgagttcg caatgtctgg
     2341 caataacgtt tctgatgggt tatttgccag cccggagcgc gatattgccg ccagtcacga
     2401 gcggtaattt tcgttatttc atcgtaattt attttatctc ctacaagatt atttaatgga
     2461 atgcaaaata tctggcctga taacaatgct ccagaattaa tacctgcagg ctatgttaaa
     2521 gttgcctcca cacccaatct acctgttctc tcgtgtgtgc cacaggcgca atacataaat
     2581 agtctggcct gtaagttcgt aacggatttc gtactcgcca gtaaaaatcc gtctgacctc
     2641 cctgggttca aactgaaaca actgttctcc catacgtgga tgagttaaca gaatgaccgg
     2701 cgcctgtgtc agggactgca ccgttctggc ggcagcaggt ttactggcca gcaccagaaa
     2761 atcatataac cgcgccaaat cagaaagtgc cttactggtc cacttcagtt ccatcagcgt
     2821 ggcaccggta acggatggtc agtactgagg ctgtccgccc aggcctgtac agcctgatgg
     2881 tcgataacct gtccggatgt cacatcgtcc agggcttcca gcgtcaggcg attacgctcc
     2941 tcctcttggg caagccatgc agaaagcgcc tgtttgataa cccagccccg ggagcgttcc
     3001 agtctggcgg ccatctggtc gactttatca gccataggta atggcacatg cgcggtaatt
     3061 acttttgttg cttgtgcggt attgtttttc atttgcatac ccgattagtt gttaatcata
     3121 tttaatcata gtgattactt tcagacggaa caaggcaaat aatgcgagtc agcgcatata
     3181 aggcggcata cggtggttac atgtagcaca ggtgtaaaac cgccgaagcc cggcgcaagc
     3241 cggtacggtc gtccgaatat caccagtccc cctccggctc tgcgaggcca tgcggcctgt
     3301 cagcgatgaa aatgcctctg tgcgcggtcg gagtggtccc gacaagggtt tacccgcagt
     3361 cggggcgtat ctccgcgtta gcgggccgtg agggccgctt acgagcgtgt atgagagacg
     3421 tacagtagtt ttgtccgctc acaccagtac aaaccagtcg aaataccggt aacgacgatt
     3481 actcttgtct ctgatgccgg tggactacga gattttattt actggcgtcc tgatgcggtc
     3541 aggactggta tggaacctgt ttatgtgata cttaatgctc ttctggattc cgggcgattt
     3601 acgcgtaaac ttattctgtt agggctgtca ggctcatttt cctatatctt cggctcaatt
     3661 gttgcgacac tgggaatggg acttgttgtc gattatctgg gatggggggc cacatttatc
     3721 gtactgatac tgtctgctgt ttttgccatc attttcacac tgatgagtcg tgaacgctca
     3781 ctggagttcg aaaaagagta attttttcag gaaaaacata aaattactga ctaaatattt
     3841 attttttgaa tcgcagggca ttccggataa ggttgatatc gtgtcacggc tgaagaccta
     3901 tggatacagt atttctggtg tggagacgga tgacggatat aaggcgcttg tccgcgcttt
     3961 tcagcttcat ttccggcaga aaaattatga cggcataatg gatgctgaaa ccgctgccat
     4021 cctgtatgca ttgctggaga agtatttccc gggtaaataa ggagtgagtc gtcagagctg
     4081 cgatatatcc ccttcctcca tgggctgtct cttatacaca aatccccacc cggggatttg
     4141 tgatgtcaga tgtcccctgt aaaactatgt tttcattgcg ctctatgaag ctgagtgagc
     4201 gtaacgactg gagctgcttc tgcggtgctc cggtggcgaa cgtgagtaag cgtaaactga
     4261 ccgccgtatg tagccattaa gcctgtattg gtaacgtagg tgcccacctt ttcaactagt
     4321 ggacacctgt tatggaacag aaagcattat ctgcagaacc ccgcagatca ttttcaaatg
     4381 agtttaaact tcaaatggtt aaactggctt cacaaccagg agcctctgtt gcccgtattg
//
LOCUS       DAJTMK010000252         4433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTMK010000252 DAJTMK010000000
VERSION     DAJTMK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.259"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1086
                     /gene="nadB"
                     /locus_tag="OO135_003934"
     CDS             <1..1086
                     /gene="nadB"
                     /locus_tag="OO135_003934"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5399567.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGANCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1071..1808)
                     /gene="trmN"
                     /locus_tag="OO135_003935"
     CDS             complement(1071..1808)
                     /gene="trmN"
                     /locus_tag="OO135_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5399568.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1940..3274
                     /gene="srmB"
                     /locus_tag="OO135_003936"
     CDS             1940..3274
                     /gene="srmB"
                     /locus_tag="OO135_003936"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5399569.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3483..4364)
                     /locus_tag="OO135_003937"
     CDS             complement(3483..4364)
                     /locus_tag="OO135_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5399570.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1126 a         1207 c         1125 g          975 t
ORIGIN      
        1 gtaggcgcgt gggtatggaa ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca
       61 gtggtactgg caaccggcgg tgcgtcgaag gtgtatcagt acaccaccaa tccggatatt
      121 tcttctggcg atggcattgc tatggcgtgg cgcgcgggct gtcgggttgc caatctcgaa
      181 tttaatcagt tccaccctac cgcactgtat cacccacagg cacgcaattt cctgttaacg
      241 gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg atggcacgcg ttttatgccc
      301 gattttgatg agcgcggcga actggccccg cgcgatattg tcgcccgcgc cattgaccat
      361 gaaatgaaac gcctcggcgc aaattgtatg ttcctcgaca tcagccataa gcccgccgat
      421 tttattcgcc agcatttccc gatgatttat gaaaaattgc tcgggctggg gattgatctc
      481 acacaagaac cggtaccgat tgtgcctgct gcacactata cctgcggtgg tgtaatggtt
      541 gatgatcatg ggcgtacgga cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc
      601 ggcttacacg gcgctaaccg catggcctcg aattcattgc tggagtgtct ggtctatggc
      661 tggtcggcgg cagaagatat caccagacgt atgcctgatg cccacggcgt cagtacgtta
      721 ccaccgtggg atgaaagccg cgttgagaac cctgacgaac gggtagtaat tcagcataac
      781 tggcacgagc tacggctgtt tatgtgggat tacgtcggca ttgtgcgcac aacaaaacgt
      841 cttgagcgcg ccctgcggcg tatcaatatg ttgcaacagg aaatagacga atattacgcc
      901 cacttccgcg tgtcgaataa tttgctggag ctgcgtaatc tggtgcaggt tgcggaattg
      961 attgttcgct gtgccatgat gcgcaaggag agtcgtggcc tgcatttcac actggattat
     1021 ccggaactgc tcacccattc cggtccgtcg atcctttccc ccggcaatca ttacataaac
     1081 agataaaaag cctgggtcag cgccgtatac gcttcggaat agttctggtc tggcccacga
     1141 atgactaagc gatcgctaaa gcattctccc gcctgcgggg agaatgccag cagcacccga
     1201 tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca aacgtaaatg ccagcccatg
     1261 cttaatgcca gctccgtaaa accattacca atctgctctg gcagcactac gcagaaaaat
     1321 ccctcttcgg taatgcactc cgccgcacag gtcagcagcg atgggtgatc aagcgtagtg
     1381 gtatagcgag cctgttcccg ttgagggatc gcgcactcta ctccctgctg atagtaaggg
     1441 gggttactga tgattaaatc gaagcgtact gtctgctgtg tgagccactg ctgaatatcc
     1501 gccgtatgga cgttaatccg ctctgcccac ggggactggt tgatattttc ctgcgcctgc
     1561 gcggcagctt cactttccag ctcaacggca tcaattatca cgctgtcact ggttcgctgc
     1621 gccagcatca gtgccagcaa cccgctaccc gcgccgatat caaggcaacg tttcacccca
     1681 gccaccggtg cccatgcgcc cagcaaaatg ccatccgttc ccactttcat cgcacagcga
     1741 tcgtgagcaa caaaaaactg tttaaaagta aatccattac gacgaagcac ggatgtagac
     1801 tgtgacatga aaataaaacc ttgcaggaaa aacggcgata gcaccgggtg agaacaatac
     1861 ccgagaagcg atatccatac aaacagatga agattgcagc cgtaacgtct ataatcagcg
     1921 ccccacacag aggtagaaca tgactgtaac gactttttcc gaacttgaac tcgacgaaag
     1981 cctgctggaa gccctccagg ataaaggttt cactcgcccg accgccattc aggctgccgc
     2041 tattccgcct gcgctcgatg gccgtgatgt actcggttct gcgccgacag gcaccggtaa
     2101 aacggcggcg tatctgctgc cagcgttgca gcacctgctc gatttcccgc gtaagaaatc
     2161 cggtccgccg cgtattttga tcctcacccc aactcgcgag ctggcgatgc aggtggccga
     2221 tcatgcccgc gaactggcga aacatacgca tctggatatc gccaccatta ccggcggcgt
     2281 agcctatatg aaccacgcgg aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac
     2341 cggacgtctg ctgcaataca taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct
     2401 gatcctcgac gaagcagacc gtatgctgga tatgggcttc gctcaggata tcgaacatat
     2461 tgctggcgaa acgcgctggc gtaaacagac cctgctcttt tcggcaacgc tggaaggcga
     2521 tgcgattcag gactttgccg agcgtctgct ggaagatccg gtggaggttt ctgccaatcc
     2581 gtccacccgt gagcgcaaaa aaattcatca gtggtattac cgcgccgatg acctggaaca
     2641 taaaaccgca ttgctggtgc atctgttaaa acagccggaa gcgacccgct caattgtgtt
     2701 tgtgcgtaag cgtgaacgtg tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa
     2761 caactgctat ctcgaaggtg agatggtaca gggcaagcgt aatgaagcga ttaaacgcct
     2821 gaccgaaggc cgcgtaaacg tactggtagc aaccgatgtt gccgcgcgcg gtatcgacat
     2881 tccagacgtc agccacgtct ttaacttcga tatgccgcgc agtggtgata cttatttgca
     2941 ccgtatcgga cgtaccgcgc gcgccggtcg taaaggcacc gcgatttcgc tggtggaagc
     3001 ccatgaccat ctgctgcttg gcaaagttgg ccgttatatt gaagagccga taaaagctcg
     3061 cgtcattgag gaactacgcc cgaaaacgcg tacgccaagc gagaagcaga ccggcaagcc
     3121 atcgaagaaa gtactggcaa aacgcgccga gaagaaaaaa gctaaagaga aagagaagcc
     3181 gcgggtgaaa aaacgccatc gcgacaccaa aaatattggt aagcgccgta aaccgagcgg
     3241 aacgggcgtg ccaccgcaaa cgacagaaga gtaatcttaa ttccgggttt aagcccggaa
     3301 ttaaaagtgc tggcaaacgc aaaactgcct gatgcgctac gcttatcagg cctacatggc
     3361 tcatgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttc acgccgcatc
     3421 cggcatgaac aaaacgcact tcgtcgacaa gttgggtgcc gggtttaagc ccggcattat
     3481 ttttatcccg gcaaaaaaca ctcttcaata cactgaataa aagtgtgcat cgccgggcta
     3541 accgcttttc cagcatggtg tgcgcacatg gccgtaatgg tctgcgactg ttcgccaaaa
     3601 ggcaattcaa ttaattctcc gctctcaagc tctttttcca ccgcaaagcg gggcaagtaa
     3661 ctgacaccga tattcgccgc gacacaacgt ttgatacttt caatgcttaa cagctcaatg
     3721 gtgttttcca ccgtgatccg ccgctgacgc agcgtgctct caaatatctg ccggaagaca
     3781 cattgcggtt cgttgataat aaagctacag gcgttatgtc ttcccggctc agtaaaatcg
     3841 acatctgcaa tttgcggtga agccaccagc accagcgatt gttcacccaa ctctcgtcgg
     3901 ttcagggcat catcattccc tacgcgataa aagacgccga catcggcctc atcattcagt
     3961 agcgcatcac ggatcacgta acagttcagc gactgcaacg ataaacgcac ttttggtgcg
     4021 cgttgccgaa aacgctgcaa cacctgcggc attcggtaag agagcaacgt ttcgcccgaa
     4081 acaacgcgta gttccccgtc cggatctgac tcctttttgg cagcctcacg aagcgtatcc
     4141 atcactcggg taagttcata aatgtgcggc agcaactttt ttccttcgcg ggtaaggcac
     4201 atccgtcggc caattttctc aaataactgg actgaaaact cctgctcaag ctgctgaata
     4261 tgaaaagtca ccgtcgattg tgtacagcac aatttttgcg aagctcgcaa aaaggaaccc
     4321 tcttccacca cggttttaag cgtaataaaa cggcgcagat ccataacccc aaacctatcg
     4381 aaaatatcga atctagaata taaaaacatt cattttttaa atgttctgtg tcg
//
LOCUS       DAJTMK010000253         4351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTMK010000253 DAJTMK010000000
VERSION     DAJTMK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.260"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..602
                     /locus_tag="OO135_003938"
     CDS             <1..602
                     /locus_tag="OO135_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR5399571.1"
                     /translation="TLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYN
                     RLNELDSLIEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
     gene            877..1059
                     /gene="tnpB"
                     /locus_tag="OO135_003939"
     CDS             877..1059
                     /gene="tnpB"
                     /locus_tag="OO135_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5399572.1"
                     /translation="MLWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEG
                     IAWQHPKRTERPGIRI"
     gene            1079..2680
                     /locus_tag="OO135_003940"
     CDS             1079..2680
                     /locus_tag="OO135_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5399573.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVL
                     APGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADA
                     YAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGS
                     PAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYILNHWNALNEFCRDG
                     WVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLRE
                     VIEKLNDWPSNQVHELLPWNFSSVK"
     gene            2680..3177
                     /locus_tag="OO135_003941"
                     /pseudo
     CDS             2680..3177
                     /locus_tag="OO135_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3336..3974
                     /gene="ospI"
                     /locus_tag="OO135_003942"
     CDS             3336..3974
                     /gene="ospI"
                     /locus_tag="OO135_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5399574.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            4089..4351
                     /locus_tag="OO135_003943"
                     /pseudo
     CDS             4089..4351
                     /locus_tag="OO135_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1226 a          947 c         1176 g         1002 t
ORIGIN      
        1 ggacgttact ggagtttggt gaaaccatcc acaaaggccg ggcagcgatg gagcgggagt
       61 tccccgaagc actggaacgg atgaaagaga gactgccacc gtatctcatt atggttctgg
      121 aaaaccagta caaccgactg aatgagctgg actcactgat agaggatatt gaaaaacagc
      181 ttaccagcgt ggcgaggcag aatgaaacct gtaagcggtt gctggatatt cctggcgttg
      241 gaccacttat tgcgacggca gcggtggcca ccatggggga agcatcagcg tttaaatcgg
      301 ggcgagagtt cgccgcatat gttggtctgg ttccaaaaca aactggctcc ggagggaaag
      361 tacgtctgct ggggataagc aaacgtggtg acacttatct caggacatta tttatccacg
      421 gtgcaagagc ggtggcatta gtagctaaag agcctggccc gtggataacc gaactgaaaa
      481 aacgtcgtcc agccagtgtg gcaatcgtcg ccatggcaaa caagctggca cgaacagtat
      541 gggcgataac cgcccatgac cgtaagtatg acaggaacca cgtcagtatc agaccatatt
      601 aatcgctgat accattaaac aatgaactct taacaaaagg gtgaatgctg aaaggttgct
      661 atggcggcca gagtgatgac aaagacaggt aagaccgtga ctcactaaac ctgaacagta
      721 ttttgggctt gaagtccgcc gtgaaaataa ggggtgagtc ggcgaattac ataggggctc
      781 gcagcgttac ggctgcaata aagccggata taaagctgca acctacccgt catgtcaaaa
      841 caatggatgc cttgcaaacg ggatgcgttc atataaatac tgtgggccga tcgtgacggg
      901 ttatgcctgt tcgccaaacg cctggaacgg ggccgcttcg tctggccggt gacccgggaa
      961 gggaaagtgc acctgacgcc agctcagtta tccatgctac tggaggggat cgcgtggcaa
     1021 catcccaaac ggacagaacg gcctggcatc cggatataac ccgtgataaa acaagggaat
     1081 gaacaatgaa ctccccgatg atattgagct gcttaaagcc atgttgcgta agcaacagag
     1141 tcggcttcga cagtatgcct gtcaggtcgc gggctatgag caggaaattg aacggctgaa
     1201 agcgcaactc gacaggttgc gtcgtatgtt gttcggccag agttcagaga aaaagcgtca
     1261 taagcttgaa aatcagatcc gacaggcaga aaaacgactg tcggaactgg aaaaccgact
     1321 gaacacagcc agaaatcttc tggaagatgc atcgtcagtc acagattcac ctgacaccag
     1381 tcccccgtca gaaaacccga tcgccagtaa gcctgaatcc ccgggacgaa aatcttcacg
     1441 aaaaccgctg ccggcagaac ttccccggga gacacatcgc cttctgcctg ctgaaaccag
     1501 ttgcccggcc tgtggaggtg ttctgaaaga aatgggggaa acaatctcag agcaactgga
     1561 tatcattaat accgccttta aagttatcga aaccatacgt cccaaactgg cctgtagccg
     1621 gtgtgatgtc atcgttcagg caccacttcc ccctaaaccg atcgaacgcg gttatgccag
     1681 tgcagggtta cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg
     1741 ccagtcagaa atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg
     1801 ggtatcagaa atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct
     1861 ggaggcagga aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg
     1921 aaagacgaaa acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc
     1981 cctgccggca gccgtctggt tcgcgtattc ggcagatcgc aaaggagaac atccgcagct
     2041 ccacctggca aagtatcagg gcgtactgca ggctgatgcc tatgcaggtt ataacgtact
     2101 gtacgaaacg ggccgggtga aggaagccgg gtgcctggcc cacgcccgcc gaaaaatcca
     2161 tgacgaggat gtgcgccgtc cgacagaaat gactcaggaa gcgctcagac ggatagcaga
     2221 gttatacgac atagaagcgg agatacgtgg cagtccggca gaggaacggc ttgcagtcag
     2281 aaaagccaga agcgtccagt tgatgcagtc attgtacgac tggatacagt tgcagaggaa
     2341 aacgctgtcg aaacatgcgg agatggcgaa ggcgttcgac tatatcctga atcactggaa
     2401 tgcgctgaac gagttctgtc gtgacggctg ggtggaaata gacaacaaca tcggtgaaaa
     2461 cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct cagacaaggg
     2521 aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga acgaagtgga
     2581 gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt cgaaccaagt
     2641 gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct acgtacttct
     2701 cggggcgctt acaccgttac tgttcattgt ccgcgctgtc attccgatga agtttatcga
     2761 catggccgta gttgttcccg gcatgagcgt ttccgttgtc gctcatgtaa acgtgttttc
     2821 cagctcactt actcttatga agccagaaaa cccggcgtta aggagcagat cgttgaaatg
     2881 gcacataacg gcgcaggggg ccgtgatacc gccaggacac tgaaaatcgg catcaatacc
     2941 atcatacgga cattaaaaag ctcccgcccc ggcggataac atctgcgcca gtcgctcacg
     3001 ctgatgttgc ccttatctgt gaacttgatg agcagtggcg cttcgtcggc agcaaggctg
     3061 caacaacact ggctctggta tgcgtacaac actaaaactg gcggtgtgtt ggcttacact
     3121 ttcgggccac ggaatgatga gacctatcgg gaactgttag ccctgtttac tccgttctgt
     3181 atatattagg ccgttggtgg taagtggatc actttttaga ctcgaatagc tgcagttcac
     3241 aaaaacagga atccggctcc gttttttgtt gtcatatcag agggatattt gttatgaaga
     3301 aaatatagtg agtatatata ctgtcaggat acaatatgat taatggggtg tcgttacagg
     3361 gaactgctgg atatgaggca catacagagg agggaaatgt aaatgttaaa aaattgctgg
     3421 aatctttaaa ctctaaaagt cttggtgata tggataaaga ttctgaattg gcagcgactt
     3481 tacagaaaat gataaatccg tctggtgggg atggtaactg ttcggggtgt gcgcttcatg
     3541 cttgtatggc tatgttaggg tatggagtaa gagaggctcc cgtacctaat gaaatatcag
     3601 agtatatgac cggatttttt cataggcatt tagagcaaat tgatagtgaa ggcatagtgt
     3661 cacatcctaa tgaaacttat agtaaatttc gtgagcgtat tgcagaaaat attttacaaa
     3721 acacttcaaa aggaagtgta gtaatgattt ctattgaaca ggccactcat tggatagctg
     3781 gatttaacga tggtgaaaaa attatgtttt tggatgttca aacaggaaag ggatttaatc
     3841 tttatgatcc tgtagaaaaa tcgccagatg catttgtaga tgaaaatagt tcggttcagg
     3901 taattcatgt atctgatcag gaatttgatc actatgctaa cagtagtagt tggaagagta
     3961 agaggctttg ctgatgctga gagcctgtac atagatttga gccggcccct tgaaacgcca
     4021 gacagtggat gtatcttaaa ataagagata ggcttggata tatgtctaac actagttcta
     4081 attttgagat gactggaact ttgctggggc tggagcttcg taaacgtaaa actcctcagg
     4141 aaaaaattgc cattattcag cagacaatgg agcctggcat gacggtgtcc catgtggcgc
     4201 gtttgcatgg tattcagccc agcctgttgc ttaagtggaa aaaataatat ctggaaggaa
     4261 gtctcactgt tgttgctgcc ggagaggatg tcgttcctgc ctcagaactg gctgccgcta
     4321 taaaacaaat aaaagagtta cagcgcttgc t
//
LOCUS       DAJTMK010000254         4324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTMK010000254 DAJTMK010000000
VERSION     DAJTMK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.261"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..213)
                     /locus_tag="OO135_003944"
     CDS             complement(<1..213)
                     /locus_tag="OO135_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5399575.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="OO135_003945"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="OO135_003945"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5399576.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="OO135_003946"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="OO135_003946"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5399577.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="OO135_003947"
     CDS             2893..3633
                     /locus_tag="OO135_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5399578.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3668..4189
                     /gene="ruvC"
                     /locus_tag="OO135_003948"
     CDS             3668..4189
                     /gene="ruvC"
                     /locus_tag="OO135_003948"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5399579.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLC"
BASE COUNT         1071 a         1075 c         1212 g          966 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgagtttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg
     1741 gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 cagagaataa ctgatatgat tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ttgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gactgtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca
     3241 ttcagcaaat gtggcggtaa cctcggtaca gatggttccg tagcctatct gttcagcaaa
     3301 aaaggcgtga tctccttcga gaaaggcgat gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc accgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgagattgc agcgactctc tgatgaggcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctatggcgt
     3721 catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac gcaccaaggt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcca gattatttcg ccattgaaca agtctttatg gccaaaaatg ccgactcggc
     3901 tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tatgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga
     4021 aaaaagtcag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgttag ctatcgcgat tacccattgt cacgtcagcc agaatgcaat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga ctgtgttaag ttataccgcc
     4201 tcggtcagtt caggctgagg cgtttcaact ccctccgcga gaatcaacac caaacccgga
     4261 tgatgccaca aactggctgg cgtgacggtt ttacgctccc acggaataga acctaagaac
     4321 caga
//
LOCUS       DAJTMK010000255         4294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTMK010000255 DAJTMK010000000
VERSION     DAJTMK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.262"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            46..2208
                     /gene="ybiO"
                     /locus_tag="OO135_003949"
     CDS             46..2208
                     /gene="ybiO"
                     /locus_tag="OO135_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR5399580.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2268..3194)
                     /gene="rlmF"
                     /locus_tag="OO135_003950"
     CDS             complement(2268..3194)
                     /gene="rlmF"
                     /locus_tag="OO135_003950"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5399581.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3470..3730
                     /locus_tag="OO135_003951"
     CDS             3470..3730
                     /locus_tag="OO135_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5399582.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            3995..>4294
                     /locus_tag="OO135_003952"
     CDS             3995..>4294
                     /locus_tag="OO135_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5399583.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          995 a         1133 c         1077 g         1089 t
ORIGIN      
        1 gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg gtggatcctg
       61 ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc cggcgttaca
      121 accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat cgaacaaaaa
      181 aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg taaagaattg
      241 atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc gaagatcgtg
      301 ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt cagccgccat
      361 tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatattac cggctccccg
      421 cataagccgt ttaatccaca aacattcagc aatgcgctga cccatttttc aatgttagcg
      481 gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct gtatcgcaaa
      541 atgggcctgt gggcgcggca aaaaaatcgt gagcgcagta actggttgca gcttccggcg
      601 atgattatcg gggcgtttat tatcgaccta ctgttactgg cactgacatt atttgtcggc
      661 caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca acaaagtttg
      721 tttctcaacg cctttgccct gattgaattt ttcaaagctg tactacgcct gattttttgc
      781 ccaaacgtgg ctgaactgcg cccgttcacg attcatgacg agaccgcccg ttactggagt
      841 cgtcgcctga gctggttaag cagtttgata ggttatggcc tgattgtggc cgtgccgatt
      901 atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat tatgctgtgc
      961 atgaccgtct gggcgttgta cctgatcttt cgcaataaaa aagagattac ccaacatttg
     1021 ctcaactttg cggagcattc gctggccttt ttcagcctgt ttatccgcgc ctttgcgctg
     1081 gtgtggcact ggctggcaag cgcctatttt atcgtgctgt ttttcttttc gttgttcgat
     1141 ccgggcaaca gcctgaaatt tatgatgggt gcaacggtgc gcagcctggc gattattggt
     1201 atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat caccctctcg
     1261 ccacatactc agcgtaacta tccggagttg caaaaacggc tgaatggctg gctttcggcg
     1321 gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcgg taatgttgct gttgagcgca
     1381 tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa aaccgtagat
     1441 atcctgatcc gtatcgcact tattcttttt ttctcggcgg ttggctggac ggtgctcgcc
     1501 agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc cagcgcccgt
     1561 acgcgtaccc tgctgacgct gtttcgtaac gcgctggcgg tgattatcag taccatcacc
     1621 atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc aggtgccggg
     1681 gcattaggtc tggctatctc gtttggttcg caaacgctgg tgaaagatat cattaccggg
     1741 gtgtttattc agtttgaaaa cggcatgaac actggagatt tggtgactat cgggccgttg
     1801 accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga taccggggcg
     1861 tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg cggtattggt
     1921 tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc caatcaggca
     1981 ctgaaagatg cggtagcgga attgatggaa aacgaagaaa ttcgcgggct gattattggt
     2041 gaaccgaatt ttgccgggat tgtcggctta agcaataccg cgtttacact gcgtgtttcg
     2101 ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag ccaggtgaaa
     2161 aaacatttcg acctggtggg cgttcgcgcg ccagtgcaga cttattaggt gctgcctact
     2221 ccgggcgcga ccccggctga accgttaccg ccgggggagc caacgcttta acgctggcga
     2281 ttgacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa gcgactctgc
     2341 ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc ggtcagagca
     2401 cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca catcacctgc
     2461 ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa aggcgacttc accaccttca
     2521 caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt cagccccagg
     2581 ttacggcgtt tacgctcact acctgcccgc gctgcagcgg cggaatcgtg gaatggcggg
     2641 ttacacaagg tcgcgtcgta ttgctcgttt ttatggatga tgccgttaaa aattgcccca
     2701 ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc actgataatc
     2761 gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg ccagccatat
     2821 tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc cagaatgctg
     2881 gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg atgaatataa
     2941 tccgcccggc ccggtactgg tgggcagaga aaaccgtcgg ggatatccca gttcgctacg
     3001 gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg attggcaaag
     3061 tctacgcttt gctccccggc gggtgtaagc gtgaggaatt gcctgagttc aggattgacc
     3121 tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg caaccccggt
     3181 ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc ataacgcgac
     3241 agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct atggtttatg
     3301 gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt ttgtaaaaat
     3361 atatcgttca aaaaataagc acatctctgt caattttttg cgtcatttgc atcgcgcagg
     3421 cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta tgaaaaagtg
     3481 cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt atgccgcgca
     3541 accgatgagt aacctggaca gcggtcagct acggcccgcc ggtaccgttt cggcaactgg
     3601 cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg aacaaggcgc
     3661 gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttcggta ctgcaaccat
     3721 ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt cgcctgtttt
     3781 gtaatcaaaa aacatcaaat atgaactcaa tgtaaataaa tgtatttctt tttcgcgcaa
     3841 tgggtgatag aaaatcgcgc caagtgataa tgcttatcag aattattatc acttgcacga
     3901 acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc gtggcagtga
     3961 aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca atttccctgc
     4021 cagacaattt cattcgctca cgttctttgc cggtctttgt attggcatca cacctgtggc
     4081 tcaggcactc gccgccgaag ggcaaactaa cgcggatgac acgctggttg tcgaagcatc
     4141 aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc gtccggtagc
     4201 ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc agggcgcaac
     4261 caaccttacc gacgcgctca aaaacgtccc cggc
//
LOCUS       DAJTMK010000256         4217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTMK010000256 DAJTMK010000000
VERSION     DAJTMK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.263"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO135_003953"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO135_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5399584.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO135_003954"
     tRNA            complement(324..399)
                     /locus_tag="OO135_003954"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO135_003955"
     tRNA            complement(406..480)
                     /locus_tag="OO135_003955"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO135_003956"
     tRNA            complement(597..681)
                     /locus_tag="OO135_003956"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO135_003957"
     tRNA            complement(690..765)
                     /locus_tag="OO135_003957"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO135_003958"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO135_003958"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5399585.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO135_003959"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO135_003959"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5399586.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO135_003960"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO135_003960"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5399587.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1088 a         1052 c          941 g         1136 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctgggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgttgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caagcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tagaaacgca aaaaggccca
     4201 gtcttccgac cgggcct
//
LOCUS       DAJTMK010000257         4212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTMK010000257 DAJTMK010000000
VERSION     DAJTMK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.264"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..438
                     /gene="tnpA"
                     /locus_tag="OO135_003961"
                     /pseudo
     CDS             1..438
                     /gene="tnpA"
                     /locus_tag="OO135_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(556..1500)
                     /gene="hypT"
                     /locus_tag="OO135_003962"
                     /gene_synonym="qseD"
     CDS             complement(556..1500)
                     /gene="hypT"
                     /locus_tag="OO135_003962"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5399588.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1845..2564
                     /locus_tag="OO135_003963"
     CDS             1845..2564
                     /locus_tag="OO135_003963"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5399589.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2630..3928
                     /locus_tag="OO135_003964"
     CDS             2630..3928
                     /locus_tag="OO135_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5399590.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1060 a          914 c         1036 g         1202 t
ORIGIN      
        1 cgcacacccg atggaactgt aaatatcaca tagttttttg cgccaaaata ccgaagacag
       61 gtgttctaca gagagaagcg tagagcaaca ggcagtattt tgagaaagct gtgtgagtgg
      121 aaaagcgtac ggattctgga agctgaatgc tgtgcagatc atatccatat gcttgtggag
      181 atcccgccca aaatgagcgt atccggcttt atgggatatc tgaaagggaa aagcagtctg
      241 atgctttacg agcagtttgg tgatttgaaa ttcaaataca ggaacaggga gttctggtgc
      301 aggggggact acgtcgatac agtgggtaag aacacggcga agatacagga ttacataaag
      361 caccagcttg aagaggataa aatgggagag cagttatcga ttccctgtcc gggcagcccg
      421 tttacggacc gtaagtaacg aagttggatg caaatgtcag atcgtgtgcg cctgttaggg
      481 cgcggctggt aagagagcct tataggcgca tttgaaaaac ctccggctat gccggaggat
      541 atttattgca cgtcgtcaat tgcggcgtgc tggctcttcc attgatatct gctggattaa
      601 atcgtctggc atatgttcct gtaaaatacg ccagaggttc tcggcggttc tattcagtcg
      661 ggtgttcatt cggtaaatat aagcctgaat ggggactgtc aatttagcag catcaaggca
      721 tatcagacgt ttggtctgta gttcatcgac gatcgaccag atcggtaacc aggcaatgcc
      781 gtatccgttg agcgccatat tctttaataa ctcgctcatc gaagagataa atatcgtgcg
      841 cccggtaata cctccaacct ctgccagatg acgattgacc aacctgccca tataagatgt
      901 ggcggtatag ttgagcaaag gaacctgggg ctgagtgata tcgaatacag ggtgcccctg
      961 tgcgtcggcg gcacataccg ggtacaactc agattcaaac agtttcaggc agcaaaaagg
     1021 cgattgcata agatcttcat cgcgaaacga aataataaag tcactcttgc cttcgcgtaa
     1081 cgtatttacg gcctggacaa catcaatggc ttcaacatga tagacaaatt ctccgccata
     1141 ggcggtaagt gaatgcacca gtttgggtaa tacgctcagg gatagtgagt gcgcggctgc
     1201 aattttaatg ttgggaacgc cgagtaaact ctggccgttt aactccccca aattgcattc
     1261 aagctgctgt aataagctgc gagtctggga atgaaagagc ttgccctctt cggttaattg
     1321 taaaggggtg gtcgtgcgat cgaataaaac aacgccgatc gctgcctcca gcgccttaat
     1381 tcgtcggctg aatgcgggtt gcgaaagatt acgctctttt gccgcctgag aaaaatggcg
     1441 acaggcttcc agtgtcagga aatcgtataa ccattttgtc tcgatattct tcattaccat
     1501 ggcacctgta ttttcaatcc aaagacataa taaaacatcc cggcaataaa aataatgttc
     1561 tcaattagca caacctggcc tttatcattc gttttgtgca taaccccttc atttccggta
     1621 ttgacgttga tatcacattt cgccaaaaaa acgaaatttt tccgtcagat aaccagaata
     1681 catcatcact atggtgaata agcggaatgt tccatagctg catcatgcgt gtcttgcatc
     1741 gccttgtaca accttgcatt tctcatcgcc catttcgact gttaaaaaag agagcagcag
     1801 ttatttaccg cgtattgtca ttaacgtttt gagaggcagg tattgtgaaa ggtttaattg
     1861 gtgtactcgg tggaatgggg ccggcagcta ccgtcgacct gtttaataaa tttgtaactt
     1921 ttaccgctgc acaatgtgat caggaacata ttcctttaat tatttcttcg atcccggata
     1981 ttcctgaccg taccgatgca ttaatgcatc atgggcgttc accattaccc gttatgcgtg
     2041 attatatgca taagctggaa gatgccgggg cagaatgtat tgttattccc tgtaataccg
     2101 cgcattactg gttcaaagaa ttaaaagatg cttgtcatat tgatatatta agtatcgtag
     2161 aaactacgat caatgaagtt cgcgcctgtg gtaaaacccg aattggcttg ttagccacca
     2221 atgccacgct atatatgggt ttataccaaa aaggaattga gtcgctggga tttacctgtg
     2281 tcagccccga tgccgacggt cagaaaaaag tgatggaaag tatttattcc ttgaaagctg
     2341 gcaatatagc gcacgcacaa aagttgatga atgagcaggc tgaaatatta ttttcccgcg
     2401 gtgcagaaat ccttgttctt ggttgtacag aaattccggt tattctggcg caagccgtta
     2461 aagagcagcc ttcccgctat attgactcaa cggcgtcact cgttcgtgcc ggaataaaat
     2521 ggtacgaaaa tcgtgtcggt aaacattatc ttttaaccca ataatcattt aaatcgcagc
     2581 cagaaagtta ttcgctttta actgaattat atttataacg gagaacatta tggtttggct
     2641 ggaaattatc gtagtacttg gtgcaatatt ttttggtatt cgccaggggg gaatcggtat
     2701 tggtttatgt ggcgggcttg ggcttgccat tctgactctg ggatttggtc tgcctatggg
     2761 gtcgccgcca gtcgacgtga tcctgattat tatgaccgtt gttgtggcgg cctcagcctt
     2821 acaggccgca ggcggaatgg attatctggt acgcgtcgct ggtaatttta tgcggcgtaa
     2881 cccgaaatat atcaacataa tcgcgccgat tatcacctgg tctatgacca ttatggccgg
     2941 tactgggttt attgtttttt ccacgctgcc ggtgattgct gaagtggcga aagagtccgg
     3001 tatccgtcct tcccgcacgc ttgccggttc ggttgtcgct tcgcaggttg cgatttccgg
     3061 ttcgccgatc agcgccgcaa tggcggctat gctcactatt atggaggtaa acggtgtcag
     3121 ttttattcaa gtgatgtcgg tatgtcttcc cacctctttt gctgccgcga tggtcgccgc
     3181 ttttattgct tcccgacagg gatgtgagtt gcaggatgac gaggtctacc ttgagcgcct
     3241 gcaaaaagga ctggtgcaaa aatacgagaa taatagcagc atcaagccag gtgcgacgct
     3301 ctccgtcgga ttgttcctgc tggcgacggt cgctattgtt attcttgctg ccttccccca
     3361 actgcgtccg ggttttgata tcggtaaacc gatggaaaca cgcgatatca ttattatctg
     3421 tatgctctct gccgcctgcc tgatggtggt gctgtgtaag acggcgaccg atgccattat
     3481 tctcacctcc acattccgtg cggggatgag ttcgctggcg gtaattctcg gcattgtaac
     3541 cttaggcacc acttttattg atgcgcattt aactgagatt aaagatattg ctggtgatat
     3601 tttgcaggcg tatccgatgt tgttggcgct ggttcttttt ggcacttgcg cgctgctcta
     3661 ctcgcaaggt gcgaccacgc cgttaattat cccgcttgcc gtcgcgcttg acgtgcccac
     3721 ctgggcaatt ctggcgtcct atgtggccgt caccggagta tttgtactgc caacgtatcc
     3781 aacatcactg gctgccatgg aatttgatac taccggcaca actcgtgtag gcaaatatgt
     3841 gctgaatcat ccatttatgc tgcctgggtt ggggggaatt attgctggag tggcattagg
     3901 ctttgtgatt gcgccaatga ttatttagca ctatcaaaca tcagtatcga tggtttatac
     3961 acaataaaga cagatatggc ggaatatagt ctgttgtaag cgccaacaaa gaatagggga
     4021 attccgccat gtctgaacat cttcacaata tggtattaag tgttgtcgtt taatgattta
     4081 tttcttggtt atgttgtgta ttagctgcat ctatcgcttt ttgatacctg aggtgtactg
     4141 gcaatagcgg acactaccat ttgttctttt tttaagcagt catctgatga tatttttccc
     4201 tgaaggctgc cg
//
LOCUS       DAJTMK010000258         4206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTMK010000258 DAJTMK010000000
VERSION     DAJTMK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.265"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            113..616
                     /gene="yeaK"
                     /locus_tag="OO135_003965"
     CDS             113..616
                     /gene="yeaK"
                     /locus_tag="OO135_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5399591.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(617..721)
                     /locus_tag="OO135_003966"
     CDS             complement(617..721)
                     /locus_tag="OO135_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399592.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            890..1336
                     /locus_tag="OO135_003967"
     CDS             890..1336
                     /locus_tag="OO135_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5399593.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEQQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1293..2114)
                     /gene="nimR"
                     /locus_tag="OO135_003968"
     CDS             complement(1293..2114)
                     /gene="nimR"
                     /locus_tag="OO135_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5399594.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNHARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2211..3407
                     /gene="nimT"
                     /locus_tag="OO135_003969"
     CDS             2211..3407
                     /gene="nimT"
                     /locus_tag="OO135_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR5399595.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDLPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGTVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3447..3794
                     /locus_tag="OO135_003970"
     CDS             3447..3794
                     /locus_tag="OO135_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5399596.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHDQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3816..4070)
                     /gene="yoaF"
                     /locus_tag="OO135_003971"
     CDS             complement(3816..4070)
                     /gene="yoaF"
                     /locus_tag="OO135_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5399597.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          970 a         1068 c         1038 g         1130 t
ORIGIN      
        1 acatgatgcg tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag
       61 ccgttcatga taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga
      121 aatggctaaa ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc
      181 taacttccgc gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg
      241 tggcaccgct ttaggccagg gagctaaggc actggtatgt aaagtcaaag gcaatggcgt
      301 aaatcaacat gttctggcga tcctcgctgc cgatcaacag gccgatctga gccaacttgc
      361 cagccatata ggcggattac gcgcttcgtt agccagccct gctgaagtcg atgaactcac
      421 aggctgtgtc ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc
      481 cgaccctcta ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa
      541 atccgttatt ttgaaaacca cggattattt gcgcattgcg caaccagaac tggtcaattt
      601 ccgccgcact gcgtaactag cctgtccgtt caataagcag aacggacaaa accagcacaa
      661 cagcaatggc aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca
      721 tagcatgccc ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt
      781 tgatgacagt tcgcctgatt tttatgcaaa aaacgtaaag attttctact tccttcctgc
      841 agcaagcgta aagtaagcag gcttattatt ttttggcaag gaaaccacga tgtttgatgt
      901 cactttgctg atcctgctcg ggttagctgc gctaggcttt atcagtcata acaccactgt
      961 cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca cctttttccc
     1021 gtggattgaa caacaggggc taagtatcgg gataatcatt ctgacgattg gcgttatggc
     1081 ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc tgaactggaa
     1141 atcgctggtg gcaattgcag taggcgtgat tgtctcctgg ctgggtgggc gcggcgtgac
     1201 cttaatgggc agccagccgc aactagtcgc cgggctgctg gtaggcactg ttttaggcgt
     1261 ggcgctgttt cgcggcgtac cggtcggccc gcttattgcc gcgggtctgg tttcgctgat
     1321 tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc caggcctttc
     1381 ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc aactttctgt
     1441 acagtatcgc ctttcactaa tccctgaagt gccataatca gctgtaactg ctgacgccac
     1501 tggcgaaaac tcaaccccgt ctcttttact atcagtcgcg catgattgcg ttcactcatc
     1561 gcaaaaaagc cagcccattg ccctaatgac ccccactcga cgggcccttt cgccatcatc
     1621 tccaccatcg tgcggatttt aggatgagaa gaaacgggca ggtgcagctg ttgttgtggc
     1681 tgctgaggga gttcatcaaa caggacctgt attaatctgc gggtcatcgg ctcagcacgc
     1741 tgagtcgtcg tcctgtttgc cagggttaaa atcaattccc ggcataaggg cgatattttc
     1801 aatgtgcaac atgttgtcgg cattgtcact gcgctgggtt cgataaacag aaaacagagt
     1861 tcagcgttag ctgtcacttg attgctatgt tccaccccgc ccggtatcca aaccgcatat
     1921 tgcggcggca ccatccacaa agcattttcc accgtacagg taattgcacc atgtaatgct
     1981 agtatcagct gtcctttgcg gtgctgatgt acaggactgg ttaactcatc cgttccggcg
     2041 tgaatacaaa atgcgaccgc ggcttcatgg tgtcggtcgg gttcatagcc attgagattc
     2101 aacctgtgca tcattttgtc cgaatttagc gataatttgt cattttagct tgattcaaca
     2161 taacaataaa aacggtaagg tacagcctcg tttgtaacaa tgagaagcat atgacctgtt
     2221 caacttcatt aagcggcaaa aacaggattg tccttatcgc tggcattctg atgattgcca
     2281 caacattacg cgtcaccttt accggcgcag caccgttact ggatacgatt cgttccgctt
     2341 actcgctgac gacagcgcaa accggcttat tgaccaccct gccattattg gcctttgcgc
     2401 taatctcacc tttggccgcc ccggtagcgc gacgttttgg tatggaacgt agcctgtttg
     2461 ccgcgttact tttgatctgt gctggtatcg caattcgctc tctcccttcg ccttacttat
     2521 tatttggcgg tacagcggtc attggcggtg ggattgcatt aggcaatgtc ttactgccag
     2581 gattaattaa acgcgatctc cctcattccg tcgccagact taccggcgca tattccctga
     2641 caatgggagc tgcggcggca cttggatcgg ctatggtagt gccgctggct ttgaacggtt
     2701 ttggctggca aggcgcgttg ctcatgctga tgtgttttcc tctgctggct ctttttttat
     2761 ggctgccaca gtggcgaagc caacaacatg caaatttgag tacctcgcgc gccttacata
     2821 ctcggggtat ctggcgttcg ccgcttgcct ggcaggtcac attgtttctt gggagcaact
     2881 cactggtcta ttacgtgatt attggctggc ttccggcgat cctcatcagt cacggctata
     2941 gcgaagcaca ggcgggttca ctgcatggtt tgctgcaact agccacagca gctcccggtt
     3001 tgctgatccc tcttttctta catcatgtga aagaccagcg tggtattgca gcgttcgttg
     3061 ccttgatgtg cgcagtgggc actgttgggc tctgctttat gccagcgcac gcgatcacct
     3121 ggactctgct tttcggtttt ggttccggcg caacaatgat actggggttg acgttcattg
     3181 gtcttcgggc aagttctgcg catcaggcgg cggcactctc ggggatggca caatccgtcg
     3241 ggtatttgct ggcagcctgt gggccgccgc taatgggtaa aatacacgat gctaacggta
     3301 actggtccgt accactgatg ggtgttgcaa tactttcact actgatggcg attttcggac
     3361 tttgcgccgg gagagacaaa gaaattcgct catatccggt gctatagtga cgtgacaaat
     3421 catgcgtgaa agggagaaca aacacgatga atattcagtg caaacgcgtt tatgatccgg
     3481 ctgaacagag cgatggttat cgcgtactgg tcgaccgcct ctggccgcgc ggtatcaaaa
     3541 aaaccgattt agcccttgat gagtgggata aagaaatcac gccgtcaacg gaactgcgca
     3601 aagcctttca cggcgaagtc gtcgatttta caacctttcg cgagcaatat cttgcagaac
     3661 tggcgcaaca cgatcaagaa ggaaagcggc tggcggacat cgccaaaaaa cagccgctga
     3721 ccctgctcta cgcagcaaaa aacaccacgc agaaccatgc gctggtgctg gccgactggc
     3781 tacgtagctt gtgattttag tacagcatcc ggcggttatt tttcaccagc cggatggtcc
     3841 cgtcgccaca atgcccattc atcaatcgtt tcaccgcccg gtaatttgca attgttgctg
     3901 accccttgcg ctgtcgacac tggaatgagc gtcccgccct tctgctggca atagaccgac
     3961 gccggatttg ccataccaat ctgcggcggt ttaggtgctt ctggctgaga aggggttgaa
     4021 caaccagcca ggaccagcaa gcaaggcaga acaaaactga taattttcat ttattgatct
     4081 cacatattta tccagactta gagtatcgcg gtatcgtttt gttttgcagc actattttta
     4141 ttacattcac tcaaaacata ttacgtcttg tttcatcttt gttgatgatg ttttatcatg
     4201 cctgca
//
LOCUS       DAJTMK010000259         4203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTMK010000259 DAJTMK010000000
VERSION     DAJTMK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.266"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..270)
                     /locus_tag="OO135_003972"
     CDS             complement(<1..270)
                     /locus_tag="OO135_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5399598.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO135_003973"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO135_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO135_003974"
     CDS             complement(1130..1336)
                     /locus_tag="OO135_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5399599.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO135_003975"
     CDS             complement(1443..2006)
                     /locus_tag="OO135_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5399600.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..4145)
                     /locus_tag="OO135_003976"
     CDS             complement(3381..4145)
                     /locus_tag="OO135_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399601.1"
                     /translation="MTSCYETLAVYICLIRKNKINDVLQHYDDMERKGLFGELPSGYV
                     RGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPVYKEGEIQYD
                     ANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILIDKEYVYDGK
                     TLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKSPVDEIKNVM
                     MLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
BASE COUNT         1340 a          923 c          752 g         1188 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccaagtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcg
//
LOCUS       DAJTMK010000260         4151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTMK010000260 DAJTMK010000000
VERSION     DAJTMK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.267"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            76..729
                     /locus_tag="OO135_003977"
     CDS             76..729
                     /locus_tag="OO135_003977"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR5399602.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            726..1052
                     /locus_tag="OO135_003978"
     CDS             726..1052
                     /locus_tag="OO135_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5399603.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENAKLRAAHWLH
                     ERGLKV"
     gene            1049..1438
                     /locus_tag="OO135_003979"
     CDS             1049..1438
                     /locus_tag="OO135_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5399604.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLNNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKPAGAAA"
     gene            1458..2255
                     /locus_tag="OO135_003980"
     CDS             1458..2255
                     /locus_tag="OO135_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR5399605.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQTADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            2263..3251
                     /locus_tag="OO135_003981"
                     /pseudo
     CDS             2263..3251
                     /locus_tag="OO135_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            3265..4017
                     /locus_tag="OO135_003982"
     CDS             3265..4017
                     /locus_tag="OO135_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5399606.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAETQLRKVTR"
BASE COUNT         1020 a          953 c         1203 g          975 t
ORIGIN      
        1 gtgcttacgg aaaagcagca agcgcgctgt atcgggtgat gagtggtatc tgtcagggaa
       61 ttacgtgggg gcttaatgag taataaatat tgccaggcgc tggtggagtt gcgaaacaaa
      121 ccagcccatg aactgaagga agtgggcgat cagtggcgca caccggacaa cattttctgg
      181 ggaattaaca ccctgtttgg cccgtttgtt ctggatctgt ttactgacgg tgataacgcc
      241 aaatgtgccg cgtattacac ggcggaagac aacgcgctgg cgcatgactg gtcagaacgt
      301 cttgcggagc ttaaaggtgc tgcctttgga aatcctccat acagccgcgc cagtcagcat
      361 gaggggcaat acatcaccgg catgcgttac atcatgaaac atgccagtgc catgcgtgat
      421 aagggcgggc gctatgtttt cctgatcaaa gctgccacca gcgaagtgtg gtggccggaa
      481 gatgcggatc atattgcttt tattcgcggg cgtattggtt ttgaactgcc tgcctggttt
      541 atcccgaaag acgagaagca ggtgccaacc ggtgctttct tcgctggtgc tattgccgtt
      601 ttcgacaaga cctggaaggg accggcaatc agctacatcg ggcgcgatga acttgaggca
      661 tgtggtgagg cctttctggc gcaagttcgc cagcaggcag aaaaacttgt cagggagatg
      721 gcggcatgac gacgttaact caatgccagc agcaggtgct ggatatgctg atttcttacc
      781 agaaagaacg tggcttcccg ccaaccaatc aggaggtggc aaccatgctg ggataccgtt
      841 cagtgaatgc agcggtggag catcttagag cactggagaa aaaaggcgtc atcacgataa
      901 agcgtggtgt ggcccgtggt atcactcttc ataccgcggt gaaggacgac gacagcgagg
      961 cggtcgggat tatccgctca ctgcttgccg gtgaggaaaa cgccaagctg cgtgcagccc
     1021 actggttaca tgagaggggc ctgaaagtat gaagctaata ctgccttttc cgcccagcgt
     1081 gaacacgtac tggcgacacc ccaacaaagg ggcgtttgct ggtaagagcc tgataagcgc
     1141 agcggggcgc aaattccaga gcgcggcgtg tgcagcaata gttgagcagt tacgtcgtct
     1201 gccaaaacca acgtcggcac ctgcttcagt ggagatcgtg ttgtttcctc cggataaccg
     1261 gatccgcgat ctgaacaact ataacaaggc gctgtttgac gccctgaccc acgcgggtgt
     1321 gtgggaagac gacagtcagg tgaaaagaat gctggtggag tggggaccgg ttatcccgga
     1381 agggaaggtc gagatcacta tcagtaagta cgagaaaccg gcgggtgcag cagcctgatt
     1441 aagaggagaa acgaagtatg aataatctga tggttattga tggaattgaa gttcgtcgtg
     1501 atgcttatgg gcgttacagc ctgaacgatc tgcacagggc tgctggttct ctggataagc
     1561 ataagcctgc attctggctc cgcaatgagc aaactgaacg tttaataagc gagttgcaga
     1621 tttgcaactc ggtcaatata gaaccagtta acgttattcg tggcggaaat aaccaaggga
     1681 cgtatgtctg caaagaactg gtgtatgcct atgcaatgtg gatcagcccg tcattccatc
     1741 tgaaggtgat ccgtactttc gacatggtaa ccagtgcacc ggaaaaatta tccggacaga
     1801 ctgctgacaa gatgcaggct ggtgtgattc tgctggactt tatgcgccgg gaattaaacc
     1861 tgtctaactc ttcagtgctt ggagcctgtc agaaactcca ggaggctgtt ggcttaccga
     1921 atctggcacc gcgctatgcc attgatgctc ctgctgacgc gcctgatggc tcaagccgcc
     1981 ccacgctgtc actgagtgca ctgctgaagc agtatggtat ccgccttacg gctaatcagg
     2041 catatcacca gatggcgaag ctggggatcg tcgaacagcg cgaacgatac agccgtacag
     2101 cgattaacaa catcaaaaaa ttctggtcgc tgacagcgaa aggctgcatg ttcggcaaga
     2161 atatcaccag tcccgcaaat ccgcgtgaga cgcagccgca tttcttcgaa tcccgattcc
     2221 ctgagctgtt aaagctgctc gataccgttc attgaggtga ccgtgagagc actactgccc
     2281 cctgaaattg ccccgcgtat ggggatcgta ttgttcagac caagttcaga gctgatgccc
     2341 ctgtttatgc aggggcgtgt cctgctggag cctgagccgg aacgttattc atctttcgcc
     2401 agtggtgctg ttccggcggc atcacaaccg ctggcggatg atcctgacgt tcgggccgtg
     2461 ttccgcaatg aggcagtgat ccgtcgtgct ggtggcgtgg aatgtcttga aagctggtta
     2521 cttcgtgaaa aaggctgcca gtggcctcat tccgactggc acagcgagaa catgacaaca
     2581 atgcgacacg cgccggcgca atccgtctgt gctggcactg cgataaccag ctgcgcgatc
     2641 agttcacgga acggctggaa tcaatggcaa cggataactg tgcccgctgg gtgttgtctg
     2701 ttgtgcgtcg ggatctcggt tttgatgata gtcacgttgt gacaatgccg gaactgtgct
     2761 ggtggctgat tcgtaatgac ctggcggatg ccttaccgga aagtgcagcc cgtaaggcac
     2821 tgagattacc gaagcctgtt gtgccgtctg tcacccggga aagtgacctt gtgccttcgg
     2881 ttcctgccac cagcatcatc caggataagg tgaaaaaggt gctggcgctg aaagtggatc
     2941 cggagtcgcc ggagtctttt atgttacgcc caaaacgtcg ccgctgggtt aatgaaaagt
     3001 acacacgctg ggttaagaca cagccgtgtg catgttgtgg aaagcctgct gatgatcccc
     3061 tccacctgat aggccacggt caggggggaa tgggtacaaa agcgcatgac ctctttgtgt
     3121 tgcctttgtg cagaaagcat cacgacgagc tgcatgcgga taccgtggca tttgaagaga
     3181 agtatggttc ccagctggag ctgatatttc gttttatcga tcgtgcgctg gcaataggcg
     3241 tactggcgta agtggagaac gagcatgaac cttgaagcct taccgaaata ttactccccg
     3301 aaatctccaa aactgagcga tgacgcaccg gcgacaggct ctggtggttt aacgattacg
     3361 gatgtgatgg ctgcgcaggg gatggtgcag tcgaaagcac cgcttgggtt tgccttattc
     3421 ctggcaaaag ttggtgttca ggatcctcag tttgcgattg aaggtctgct caattacgcg
     3481 atggcactgg ataacccgac attgaataaa ttgagtgaag aaacccggct acagatcatt
     3541 ccttaccttg tgaattttgc ctttgctgat tattctaggt ctgcggcaag taaggctcgc
     3601 tgtgagcatt gtgctggtac tggatttcat aatgtattgc gcgaggtggt gaaacactcc
     3661 agaagcgggg aatctgttat caaggaagag tgggtgaagg aactatgtca gcattgtcat
     3721 ggtaagggag aagtcagcac agcgtgcaga gggtgtaagg gtaaaggtat tgtcctggat
     3781 gaaaaaagga cccggcttca tggcacgcct gtttataaga tttgtgggcg ttgcaatgga
     3841 aaccggttta gccgtttacc aactacactg gcacgacgtc atgtccagaa gctggttcca
     3901 gacctgactg attatcagtg gtacaaagga tatgcagacg taattgataa actagtaaca
     3961 aagtgctggc aggaagaagc atatgctgaa acacaattga gaaaggtgac gagataagtg
     4021 attttcgccg aagatggcga catgaagctt gcatttttca aaaaacatgg ataagatttt
     4081 ctcaacgatg ggctttgtat gtctgccgtt gataattttc acgaacccgc tgttgagcgg
     4141 gtttttattt a
//
LOCUS       DAJTMK010000261         4046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTMK010000261 DAJTMK010000000
VERSION     DAJTMK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.268"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..305
                     /locus_tag="OO135_003983"
     CDS             <1..305
                     /locus_tag="OO135_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5399607.1"
                     /translation="HGQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIY
                     CGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHQAWHASAFTGIYNDLVAASTFV"
     gene            242..844
                     /locus_tag="OO135_003984"
     CDS             242..844
                     /locus_tag="OO135_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5399608.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLNE
                     GWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVASQEISTADEGDGGQVVVIGR"
     gene            917..1255
                     /locus_tag="OO135_003985"
     CDS             917..1255
                     /locus_tag="OO135_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399609.1"
                     /translation="MNKTILFCTIIALTGCKSLDYVKSGKPVMEGNSLKNIDELSGCI
                     SRQWAGNGTPITSLPIENGVSLLVPQAMGGYDIVLDIKKAGNGSSFTLYERVPALTPK
                     IFADSVNACK"
     gene            1322..>4046
                     /locus_tag="OO135_003986"
     CDS             1322..>4046
                     /locus_tag="OO135_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR5399610.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRYMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYMLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRKVETTARYLGTGLYWIAASINIKPGHDYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDKGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLKDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGI"
BASE COUNT          957 a          976 c         1237 g          876 t
ORIGIN      
        1 gtcacggtca gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt
       61 tgtcagcggc gcagccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc
      121 atgccgccat ttactgtggt gacggcgagc tgctgcacca tattcctgaa caactgagca
      181 aacgagagag gtacaccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccagg
      241 catggcacgc atctgccttt acggggattt acaacgattt ggtcgccgca tcgaccttcg
      301 tgtgaaaacg ggggccgaag ccatccgggc gctggccaca cagctcccgg cgtttcgcca
      361 gaaactgaat gagggctggt atcaggtgcg cattgccggg cgtgatgcag gcgaaaacga
      421 attatctgcc cgtcttaatg agccgctggc aaatggtgcc gtgatccaca tcgtaccgcg
      481 tctggtggga gctaaaagtg gcggtgtgtt tcaggcggtg ctgggggcag ctgttatggc
      541 ggttgctata tggatgccgg gggtaggaat tatggcgagt aatctgctgt tttctctcgg
      601 tgccagtatg acgcttggcg gtgttgcaca gatgctggca ccgaaagcca gaactccccg
      661 tacacagaca acggataacg gcaaacagaa cacctgtttc tcctcactgg ataacatggt
      721 tgcccagggc aatgtcctgc ctgttctgta cggtgaaatg cgcgtggggt cacgtgtggc
      781 atctcaggag atcagcacgg cagatgaagg ggatggtggt caggttgtgg taattgggcg
      841 gtaatattat ttactcatgt tctaactgat ttaatattta tatcgaacac tgataattat
      901 tctattggtt agctatatga acaaaacgat tttattctgc acgattattg ccttaacagg
      961 atgtaaatct ttggattacg taaaatccgg aaaacctgta atggaaggta attcattaaa
     1021 aaatattgat gaattgtcag gctgcatatc cagacaatgg gctggtaatg gaacacctat
     1081 aacatccctt cctattgaga atggggtaag ccttttagtt ccacaggcta tggggggata
     1141 tgatattgtg cttgatatca aaaaagcagg aaatggcagt agttttactc tttatgaacg
     1201 tgtaccagca ttaacgccaa aaatttttgc tgatagtgtt aatgcatgta aataatagtt
     1261 aatcctgccg taactcatga gccgcctttt gggcggcttt gttgtttatg gagtgtgagg
     1321 aatgggtaaa ggaagcagta aggggcatac cccgcgcgaa gcgaaggaca acctgaaatc
     1381 cacgcaatta ctgagtgtga tcgatgccat cagcgaaggg ccgattgaag gtccggtgga
     1441 tggattaaaa agcgtgctgc tgaacagtac accggtgctg gacagtgagg ggaataccaa
     1501 catctccggt gttacggtgg tgttccgggc aggtgagcag gagcagacac cgccggaggg
     1561 atttgaatcc tccggttccg agacggtgct gggtacggaa gtgaaatacg acacgccgat
     1621 cacccggacc atcacgtctg caaacatcga ccgtctgcgc tttaccttcg gtgtgcaggc
     1681 actggtggaa accacctcaa agggtgacag gaatccgtcg gaagtccgcc tgctggttca
     1741 gatacaacgt aacggtggct gggtgacgga aaaagacatc accattaagg gcaaaaccac
     1801 ctcgcagtat ctggcctcgg tggtggtggg taacctgccg ccgcgcccgt ttaatatccg
     1861 gatgcgcagg atgacgccgg acagcaccac agaccagctg cagaacaaaa cgctctggtc
     1921 gtcatacact gaaatcatcg atgtgaaaca gtgctacccg aacacggcac tggtcggcgt
     1981 gcaggtggac tcggaacagt tcggcagcca gcaggtgagc cgtaattatc atcttcgcgg
     2041 gcgcattctg caggtgccat cgaactataa cccgcagacg cggcaataca gcggtatctg
     2101 ggacgggacg tttaaaccgg catacagcaa caacatggca tggtgtctgt gggatatgct
     2161 gacccacccg cgctacggca tggggaaacg tcttggtgcg gcggatgtgg acaaatgggc
     2221 gctgtatgtc atcggccagt attgcgatca gtcagtgccg gatggctttg gtggcacgga
     2281 gccgcgcatc acctgtaacg cttacctgac cacacagcgt aaggcgtggg atgtgctcag
     2341 tgatttctgt tcggcgatgc gctatatgcc ggtatggaac gggcagacgc tgacgttcgt
     2401 gcaggaccga ccgtcggata aggtgtggac ctataaccgc agtaatgtgg tgatgccgga
     2461 tgatggcgcg ccgttccgct acagcttcag cgccctgaag gaccgccata atgccgttga
     2521 ggtgaactgg attgacccgg ataacggctg ggagacggcg acagagcttg ttgaagatac
     2581 gcaggccatt gcccgttacg gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag
     2641 ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa acggagctgc tggaaacgca
     2701 gaccgtggac ttcagcgtgg gtgcagaagg gcttcgccat gtaccgggcg atgtcattga
     2761 aatctgtgat gatgactatg cgggtatcag catcggcggg cgcgtgctgg cggtgaacag
     2821 ccagacgcgg acactgacgc tcgaccgtga aatcacgctg ccatcctccg gcaccacgct
     2881 gataagcctg gttgacggac aggggaatcc ggtcagcgtg gaggtccagt ccgtcaccga
     2941 cggcgtgaag gtgaaagtga gccgtgttcc tgacggcgtt gccgaataca gcgtgtgggg
     3001 gctgaagctg ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc gtgagaacga
     3061 tgacggcacg tatgccatca ccgccgtgca gcatgtaccg gaaaaagaag ccatcgtgga
     3121 taacggggcg cactttgacg gcgaccagag cggcacggtg aatggtgtca cgccgccagc
     3181 ggtgcagcac ctgactgccg aagtcaccgc agacagcggg gagtatcagg tactggcccg
     3241 ctgggacacg ccgaaggtgg tgaagggcgt gagcttcctg cttcgcctga ccgtgacagc
     3301 ggacgatggc agtgagcggc tggtcagcac ggcccggacg acggaaacca cataccgctt
     3361 caggcagctg gcgctggggc gttacatgtt gacggtccgg gcggtaaatg cgtggggaca
     3421 gcagggcgat ccggcgtcgg tatcgttccg gattgccgca ccggcagcgc cgtcgcggat
     3481 tgagctgacg ccgggctatt ttcagataac cgccacgccg catcttgccg tttatgaccc
     3541 gacggtacag tttgagttct ggttttcgga aaagcggatt accgatatca ggaaggttga
     3601 aaccacagcg cgctatcttg gcacggggct gtactggata gccgccagta tcaatatcaa
     3661 accgggccat gattattatt tttacgttcg cagtgtgaac accgttggca aatcggcatt
     3721 cgtggaggct gttggtcagc cgagtgatga tgcatccggt tatctggatt ttttcaaagg
     3781 cgagataggg aaaacccatc tggctcagga gctgtggacg cagattgata aaggtcagct
     3841 tgcgcctgac ctggctgaaa tcaggacgtc cattacggat gtcagcaatg aaatcacgca
     3901 gactgtcaat aagaaactga aagaccagag tgcggcaatc cagcagatac agaaggttca
     3961 ggttgataca aataataatc tgaacagcat gtgggctgtg aagctgcaac agatgaagga
     4021 cggacgcctt tatattgcgg gtatcg
//
LOCUS       DAJTMK010000262         4036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTMK010000262 DAJTMK010000000
VERSION     DAJTMK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.269"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..603)
                     /gene="mcbR"
                     /locus_tag="OO135_003987"
     CDS             complement(<1..603)
                     /gene="mcbR"
                     /locus_tag="OO135_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5399611.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCR"
     gene            complement(801..1838)
                     /gene="curA"
                     /locus_tag="OO135_003988"
     CDS             complement(801..1838)
                     /gene="curA"
                     /locus_tag="OO135_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5399612.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPRTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            2019..2537
                     /gene="mddA"
                     /locus_tag="OO135_003989"
     CDS             2019..2537
                     /gene="mddA"
                     /locus_tag="OO135_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5399613.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            2534..2983
                     /locus_tag="OO135_003990"
     CDS             2534..2983
                     /locus_tag="OO135_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5399614.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFCELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARHSF"
     gene            complement(2984..3217)
                     /locus_tag="OO135_003991"
     CDS             complement(2984..3217)
                     /locus_tag="OO135_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5399615.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(3303..3476)
                     /gene="ortT"
                     /locus_tag="OO135_003992"
     CDS             complement(3303..3476)
                     /gene="ortT"
                     /locus_tag="OO135_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5399616.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            3671..3766
                     /gene="yncL"
                     /locus_tag="OO135_003993"
     CDS             3671..3766
                     /gene="yncL"
                     /locus_tag="OO135_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5399617.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          987 a         1018 c          914 g         1117 t
ORIGIN      
        1 tctgcatccc tctttgtctt ttgcttttaa cgcagcgagt aacagatgat agttttctat
       61 atgctcccgt aattccgctg gattaatcgc ttcatagaga taatgtaaac caggccccat
      121 cctgacccac agttgctcaa tcatctcaca caggatgggc atatttgagc gatgataaat
      181 cgctaagcga aatagcctgt ttacattaat gatttgttcc atgtcgccct tttcttgcgc
      241 ctgctgtaat ttctcgagca attcctggag ttccgccagg tcttgcgggg tgaggttttc
      301 aacagccaga gcaactgcca ttaattccag ttcgtagcgg atccgattga tttcgtctaa
      361 ttgacgtttc cccacttccg gaactgtaaa tgcttgtgca ggtgcgacag aaagcgcatt
      421 caccgaaacc agacgtaata atgcttcacg cacaggtgta atactcatac ctaattgctc
      481 cgccagattt ttagtaatca ggcgtgcgcc aggttttaat gcgccaatac taagctgatg
      541 tttcaggtcg ttctcaacct gcagagtcaa actgacatgt ttcatttttc ccattccagg
      601 cattttaatt ccttcgcgtg ttttctgtcc aaccctgcct gcacggcaga aggagttcac
      661 ctggtgtatt aaagtgaata atcataaacc acccaccttt gttgttatta gcagcagaac
      721 aatgacgtgg ttagttaatt aaagctgaaa ataagctttt tgcttggatt tttatggcgg
      781 cttacgccgc cgttgaaact ttaatcatca cccgcgacgc ggatcaccac tttgccgaag
      841 tttttaccct tcagcaggcc gataaacgtc cgtggcgcat tctctaaacc atcagtaata
      901 tcttcgtggt agtggatttt atcctctttc acccattgcc ccatctcctt ctgaaactca
      961 tggatgcggt gaccataatc ctgagcgata ataaaccctt gcaagcgaat acgttttttc
     1021 agcaccgttg ccatcaacag gggtaaacga tccggaccgg gtggtagctc tgtagcgtta
     1081 tagctgctca ctaagccaca aacgggaatg cgcgcagatg tattaagtaa tggtagcact
     1141 gcatcaaata ccttaccgcc cacgttttca taatagatat caataccttt tgggcacgct
     1201 ttcaccagtt gttcggcaaa atcacccgcg tggtgatcaa gacaaacatc gaagcccagc
     1261 acctcgatag catggcgaca tttttccgcg ccaccggcac cccccaccac cctgcaacct
     1321 ttaagtttgc cgatttgccc caccgttgct cccacaggac ctgtcgccgc agctaccacc
     1381 aacgtttcgc cctctttagg ctgaccgata tccagcagcc ccatataagc ggtaaagcct
     1441 ggcatcccca gcacacccag cgaccacgat ggattttgcg gatgatcgcc aagtttcacc
     1501 agatcatcac cactggatat gtcatagtcc tgccatccac tgtagcccag cacccagtcg
     1561 ccaggctgat aatcaggatg attcgactcc acaacatggc tcaccgtgcc gccgaccatc
     1621 acgccgccaa tatcaacagg cggtgaataa gatggctcat cgctcatacg tccacgcata
     1681 tacgggtcca gggacaaata aactgtgcgc agtaacacct gtccttcacc cggtgtggcg
     1741 acatcatctt cttcaagacg gaaattctcc ggaaccggtg cgccatgtgg acgcgaggcc
     1801 agaacccaac gtcgattacg ctgcttttgt tgccccataa tgctctcctg tgcttttggc
     1861 atgaatccat caatagtagc aggctcacgg gattggtttt gtttgcagcc gtcacatccc
     1921 ctctgcaaca cgcaataatt tcatcgagga atccgttgct tcgggctgtt ttcgatagta
     1981 ttgataacat catcatcatt attttatcga ggccagacat gtccatccgt tttgcccgca
     2041 aagccgattg tgctgccatt gcggaaattt ataaccacgc cgtgttgtat acggcggcta
     2101 tctggaatga ccaaacggtt gatgcagata accgcattgc ctggtttgaa gcgcggacca
     2161 tagcaggtta tccggtgctg gtaagtgaag aagatggcgt agtgacggga tatgcctcgt
     2221 ttggcgactg gcgtagtttc gatggttttc gccataccgt ggaacattcg gtttatgtcc
     2281 atcccgatca tcagggcaaa ggtctggggc gtaaattgtt aagccgattg attgatgaag
     2341 cgcgggatag cgggaagcat gtcatggtcg ccgggatcga atcgcaaaat caggcctcgc
     2401 tgcatctgca ccattcgctg ggatttgtcg tcaccgcaca aatgccgcag gtgggcacta
     2461 aatttgggcg ctggctggat ctgacattta tgcagttgca actcgacgaa cgcactgaac
     2521 cggacgcgat tggatgaatc agtcgctcac ccttgctttt ttgattgccg ccggaattgg
     2581 tctggtagtc cagaacacgc tgatggtgcg catcacccag acctcctcta ccattctcat
     2641 cgccatgttg ctgaactcac tggtggggat tgtgctcttt gtttctattt tatggtttaa
     2701 acaaggcatg gcggggtttt gcgaactggt gtccagcgtg cgctggtgga cacttatacc
     2761 cggcttgctg ggatcgtttt ttgtcttcgc cagtatcagc ggatatcaga atgtgggagc
     2821 ggcgacaact attgcagtac tggtcgccag ccagctaatt ggcggtctga tgctggatat
     2881 cttccgcagt catggcgtac cactacgggc gttgttcggc ccaatttgtg gcgcaatttt
     2941 acttgtggtt ggcgcctggt tggtagccag acactcattt taatcagagg atggtgccgc
     3001 ctttggttaa ctgttcgtgg cgcgcttcca tatcttcttt atgcttgcga ccgtgatgag
     3061 caatagcggt gcgcaaacgc tgctgctggg tgtagcgatc ttcccgactt aactccgcgt
     3121 catcgctcag ggcgattaat aattcgttca tatgggcaat gacgctctct tcaatcgctg
     3181 catccacgcg cgcgatgact tcatccagat gcgacatact ttctccttaa ccacttgtga
     3241 aatttatcga ttcatccaaa tgatataagt ctgcgtggtg ccgtcaagcc agtgagtaag
     3301 atttaaaaca gtgaaaacaa cagcgccacc gggaaactca tcggccatgt tgcccccacc
     3361 agaaaggcgc ttaagaaacg gatgcgtttc ttatcgtgag aaagaaacca ggtgataaga
     3421 aatgcgattg ctgccataac cgcataaaaa acaagcatat gttgatagag agacatgtac
     3481 gggcgtccag ccagatgaat aaaacgcgcg caaatatgcg tcattttttg acgtgcatca
     3541 agttttttca ccagtttgag aaaaaatcat caaacagcaa cttgccctta tccatcttca
     3601 gaatgagtat tattgcggat tttcttaacc cgtactatac acataggggc aatgataaaa
     3661 ggtggcaaaa atgaatgttt ccagtagaac tgtagtactg ataaatgtct ttgctgctgt
     3721 tggtttgttt actcttatct ctatgagatt tggctggttt atttgatgta gtgattttgc
     3781 acgcagcacg tactgtcaga gaaatcaact ctctgacagt acgcaagatc ttctcatact
     3841 ctccaggttt acaacattat ccttaatatt tacttctttt tgctggcatc tacattgaaa
     3901 tgagtagaaa acatccccct actgtcgtct atacaaacag cttactgatt caaacggaat
     3961 cttgttttca catttttcta tcatgaatac tttatacgcg cgggagtcta taaatgctga
     4021 ggtagcctga gtttaa
//
LOCUS       DAJTMK010000263         3980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTMK010000263 DAJTMK010000000
VERSION     DAJTMK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.270"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..2372)
                     /locus_tag="OO135_003994"
                     /pseudo
     CDS             complement(1..2372)
                     /locus_tag="OO135_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            complement(2433..3035)
                     /locus_tag="OO135_003995"
                     /pseudo
     CDS             complement(2433..3035)
                     /locus_tag="OO135_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(2972..3715)
                     /locus_tag="OO135_003996"
     CDS             complement(2972..3715)
                     /locus_tag="OO135_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399618.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            complement(3720..>3980)
                     /locus_tag="OO135_003997"
     CDS             complement(3720..>3980)
                     /locus_tag="OO135_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5399619.1"
                     /translation="SASFVLATPTETDGVVFPGHIMLANTCMWTYRSDECGYTGRAVA
                     DEFDKPTTDIRKDKCSKCMRGCELRNNTGNFGGFLSINKLSQ"
BASE COUNT          819 a         1300 c         1011 g          850 t
ORIGIN      
        1 actgcgaacg taaaaataat aatcatggcc cgcctgatat tgatactggc ggctatccag
       61 tacagcgccg tgccaagata gcgggcgctg gtttcaacct gcctgatatc cgcaatccgc
      121 ttttccgaaa accagaactc aaactgcacc gtcgggtcat atatagccag tttcgggacc
      181 gccgttatct gaaaataccc cggtatcagt tcaatagtga caggcgctgc cggtgccgca
      241 atccggaacg ataccgacgc cggatcgccc tgctgccccc gggcatttac cgcccggact
      301 gtcagactgt aattccccag cgccagctgc ctgaagcggt atgtggtttc cgtcgtcctg
      361 gctgtgctga ccagccgctc actgccgtcg tccgctgcca cggtcaggcg aagcaggaag
      421 ctcacaccct tcaccacctt cggcgtgtcc catcgcgcca gcacctgata ttccccgctg
      481 tctgcggtga cttcggcggt cagatgctgc accgctggcg gcgtgacacc attcaccgtg
      541 ccgcgccggt caccgtcaaa gtgtgccccg ttatccacga tggcctcttt ttccggcaca
      601 tgctgcacgg cggtgatggc atacgtgccg tcgtcgttct cacggatact cacgcagcgg
      661 aacaggcgct ggcgcagcgt cggcagcttc agcccccaca cgctgtattc tgcaacgccg
      721 tcaggaaccc ggctcacttt caccttcacg ccgtcggtga cggactgaac ctccacgctg
      781 accggattgc catttccgtc aaccaggctt atcagcgtgg taccggagga cggcagcgtg
      841 atttcacggt cgagcgtcag cgtccgggtc tggctgttca ccgccagtac gcgaccaccg
      901 gtgctgatac cggcataatc atcatcgcag atttcaatga catcacccgg tacatggcga
      961 agcccttctg cgccgacgct gaagtccacg gtctgcgttt ccagcagttc cgttttaatc
     1021 agccacagcc cggcgcggtg cgcctgtccc cggctggtac agccaaaggc atccatcttc
     1081 gtggcgttac gaccgtaacg gagaatggcc tgcgtgtcct ccacaagctc tgtcgccgtc
     1141 tcatgaccgt tgtccggatc aatccagttc acctcaacga cattatggcg gtccttcagg
     1201 gcgctgaagc tgtagcggaa cggcgcgcca tcatccggca tcaccacatt actgcggtta
     1261 taggtccaca ctttatccga cggtcggtcc tgcacgaacg tcagcgtctg cccgttccat
     1321 accggcatac agcgcatcgc cgagcagaaa tcactgagaa catcccacgc cttacgctgt
     1381 gtggtcaggt aagcgttaca ggtgatgcgc ggctccgtgc cgccaaatcc gtccggcact
     1441 gactggtcgc agtactggcc gatgacatac agcgcccatt tgtccacatc tgccgcacca
     1501 agacgtttcc ccatgccgta gcgcggatga gtgagcatgt cccacagaca ccaggccatg
     1561 ttgttgctgt atgctggctt aagcgttccg tcccagatac cgctgtattg ccgcgtctgc
     1621 gggttatagt tcgacggcac ctgcagaatg cgcccgcgaa gatgataatt gcgactcacc
     1681 tgctggttac cgaactgctc tgaatccacc tgcacgccga ccagcgccgt gttcgggtag
     1741 aactgtttca catcgatgat ttcggtgtac gacgaccaga gcgttttgtt ctgcagctgg
     1801 tctgtggtgc tgtccggtgt catcctgcgc atccggatat taaacgggcg cggcggcagg
     1861 ttacccatca ccaccgaggc cagatactgc gaagtggttt tgcccttaat ggtgatgtct
     1921 ttttccgtca cccagccacc gttacgctgt atctgaacca gcaggcggac ttccgacgga
     1981 ttccggtccc cctttgaggt gttttccacc agtgcctgca caccgaaggt caggcgcaga
     2041 cgatcaatgt ttgccgacgt gatggtccgg gtgatcggcg tgtcgtattt cacttccgta
     2101 cccagcacag tctctgagcc ggaagattca aacccctccg gcggtgtctg ctcctgctcg
     2161 ccggcgcgga acaccaccgt aacaccggaa aagtttgtct tcccctcgct gtccaggacc
     2221 ggcgtaccgt tcagcagcac gctttttaat ccatccaccg gaccgtcaac cggcccttcg
     2281 ctgatggcat caatcacact cagcaactgc gtggatttca ggttgtcctt cgcttcgcgc
     2341 ggggtatgcc ccttactgct gcctttaccc attcctcacg ctccataaac gacaaaaccg
     2401 cccgcaggcg gtttcacatg aaacattttg catcagcgac caatcaccac aacctgacca
     2461 ccatcccctt cgtctgccgt gctgatctcc tgagatacca cccgcgaccc cacgcgcatt
     2521 tcaccgtaca gaacaggcag aacattgccc taggcaacca tgttatccag tgaggagaaa
     2581 caggtgttct gtttgccgtt atccgttgtc tgtgtacggg gagttctggc tttcggtgcc
     2641 agcatctgtg caacaccgcc aagcgtcata ctggcaccga gagaaaacag cagattactc
     2701 gccataattc ctacccccgg catccatata gcaaccgcca taacagctgc ccccagcacc
     2761 gcctgaaaca caccgccact tttagctccc accagacgcg gtactatgtg gatcactgca
     2821 ccatttgcca gcggctcatt aagacgggca gacaattcgg tttcacctgc atcacgcccg
     2881 gcaatgcgca cctgatacca gccgtcactc agtttctgac gaaacgccgg gagctgtgtg
     2941 gccagtgcgc ggatggcttc agcccccgtt ttcacacgaa ggtcgatgcg gcggccaaat
     3001 cgttgcaaat ccccgtaaag gcagatgcgt gccattcccg gtgacgccag agggagtgtg
     3061 tgcgtcgctg ccatttgtcg gtatacctct ctcgtttact cagttgttca ggaatatggt
     3121 gcagcagctc accgtcgccg cagtaaatgg cggcatgatt cggcaccgat gaaccaaaac
     3181 agcacagcag cacatcgccc ggttgtgctg atgacaacgg cacctgatac agccctgtgg
     3241 cctccagatt atccagatag agattctgac cgtgacgcca ccagtcatcc ccgcgatgaa
     3301 aatccggcat ctcaatcccc gccagatgat aagcatcccg gaacagcgtg taacagtccg
     3361 tcaccccgtg ctcaaagcgc cgcccggtga gatgcggcac acagcggaac ttgtgaatcg
     3421 ccccccggca gaccagccac cacggcaaat cactctgcac ctgcagccgc cggtcagcct
     3481 cactcagcca gggcagacca ccgggatgac tgtggaccag tgccacaatc tcaccctgca
     3541 tttgtgcccg cagccagtcc tccggagcca tccggaaccg ttcctccggc tcaccggaga
     3601 tattctcgct gggaagatac ctgtctccct ccggcgttct caccacgaag ccacacgact
     3661 ccgctggcgc acatcgccgg gcgtgcgcca gaatcgctga ttctgtctgt gtcatggatt
     3721 tactgagaaa gtttattgat ggaaaggaaa ccgccgaaat taccggtatt gttgcgcaac
     3781 tcacacccgc gcatacactt gctgcatttg tccttccgga tatccgtcgt tggtttgtcg
     3841 aactcgtcag ccactgccct gcccgtgtaa ccacactcat cagaacggta ggtccacata
     3901 caggtattcg ccagcatgat atgccccggg aaaaccacgc catccgtctc cgttggtgtg
     3961 gccagcacaa aagaggcact
//
LOCUS       DAJTMK010000264         3915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTMK010000264 DAJTMK010000000
VERSION     DAJTMK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.271"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..2032)
                     /locus_tag="OO135_003998"
     CDS             complement(<1..2032)
                     /locus_tag="OO135_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5399620.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEFIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVWLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWTNNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGVASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAP"
     gene            complement(2048..2722)
                     /locus_tag="OO135_003999"
     CDS             complement(2048..2722)
                     /locus_tag="OO135_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5399621.1"
                     /translation="MKGLLSLLISSMIFPVHAGMVIFGTRIIYPAEQKEVMVQLMNQG
                     NRSSLMQAWIDDGDTSLPPEKIQVPFMLTPPVVKVAANSGQQIKIKIMPNKLPSNKES
                     IFYLNVLDIPPNSPKQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLINRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(2810..3352)
                     /locus_tag="OO135_004000"
                     /pseudo
     CDS             complement(2810..3352)
                     /locus_tag="OO135_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(3651..>3915)
                     /locus_tag="OO135_004001"
     CDS             complement(3651..>3915)
                     /locus_tag="OO135_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HCR5399622.1"
                     /translation="SGIIFLAYGLASPAFSSETATSTINGRISSPTCSMDVVNNHLQQ
                     RCGQLLQRVETNYRASSTAKGVTTEVVTVGNNDKRKIVLNRYD"
BASE COUNT         1147 a          922 c          720 g         1126 t
ORIGIN      
        1 ctggcgcatt catcacagca aacgtttcgg aaagacggtt cgccagatta acgccacccg
       61 accaggcgac aatgccccct gaaacactgg ctccagcctg tcgataagta ctcgactgac
      121 tataactgcc attcactgtc gcaaccggcg cgttccaggt caaattcgcc ccagctgtcg
      181 tttcatttcc ctgatgctga taactcaggt tgaccccata gttaaactga tcccggcttc
      241 caacggttcc tgataatccc gtattatttg aggcaacccc ctgatcatca aacgtcgttg
      301 agttagacat atatatttgc cgacgaggcg tcgtaacgtc atcaccccaa tcaaagggaa
      361 tcgatataaa aatattaaaa cgtttctctt catgatgatt ctcgtcatac gcctggcttg
      421 ccgcgagggt atagcttatc cgccgccagt tattggaata actcaactga taatccttac
      481 tgctgccgct acgcccccag taatctcgcc ataacgtact taaggacaca gacccccagc
      541 cttctggcaa tgactggctc atattggcag aaaagctatt tttacggcca aaatcgttct
      601 ggtaataatc ggcaatgtca tagatatcgt tttcatcacg gcgataatta tctttattgt
      661 ttgtccaaac gtgatcgtta aatgtccggt aatcacgcga cgaataacgc caggccgcca
      721 gaccaaaacg cgtcgacgtt tggctcacaa atttgttgta ggcaatttga taactttgcc
      781 cgtcaaacac atcgccgttg tcttgtttac tatgcgactt cgtggcatcg acggaaatgg
      841 caccaatgcg ggtattccag ccagtcccca aagtgaacgc gtaataatta ttcgcgacca
      901 tcgagccacc atacagcgtc aataaattat taaaaccata ctgataaccc gcctggacaa
      961 aatcactttg tttgctcgcc ccttcaatat ggctacgacc cgccgcaaaa tcatatttcg
     1021 atacgccggg ttgcagcata tttggcaccg ctgcataagg taccagatag gtggtaaccg
     1081 agccgtccgc ctctttcacg ctgacatcaa gatctgctcc accaccggcc aactgcaaat
     1141 ctgtaatcgc aaacgggcca ggaggaacct ctttctgata aacgacaaaa ccattctgtt
     1201 caatagttac cagcgcgtta ctctgagcaa tcccctgcac ccgtggcgta aaattttgtt
     1261 tcgagttagg caacatctgc atatcacgaa acaaccacac accgctgaag cgaacagaat
     1321 caaaaatatc gcttgatgtg tacatatcac ccacgcgaag cgtgccgaga agttgggcaa
     1381 atccacgttc cagatacagg gtattgcttt tccacacccc tggattgtta tttgttttac
     1441 tgaaactggc atcagaatgc agttgccacc ccagtaaatt taacccgctg ttaaaacgta
     1501 catatgtact cttgttatta cccgacgctt tatagtcgct gtaatactga ctcacataat
     1561 aagaggtata aaacgcatta ataccccgct cccagttttc cggtggaaca tagccacttt
     1621 ccagttcttc cacccaagcc tgcgggacac tgaaatcgag acgaaaaacc ccgatatccc
     1681 agctatagct cccaccctga acaagttgct caaatgttaa acattgctta ccactggcga
     1741 agttatcgct attaatgcct aaccgcttga taacttctct tgataaacat gtttcttgcg
     1801 ggttgtcttt aacaatgaac tcatatttcc cgcgccattg cttattgaca taaatatcga
     1861 tgtcatactg ccccggcaag ggttgattat catcaagacg aatatttgca acctgctggt
     1921 ctttcattcc acctatcata aaatgggtat caaaggtttc ttcagctgca taagcttcaa
     1981 tgccgagcaa taacgctacg attgctgatg caagtggggt cattctcaac ataggcagct
     2041 cctgcaatta aattttgtca ctaatatagt tgccgtggtc atcgataatg gtcagatacc
     2101 agttgtttgc attattactt ttgacattaa cactctgact ttctaagggg gcaatcataa
     2161 tagtttcata attgactttg acattattag ctttgacatc cgaaatcgtc acccaattag
     2221 ctgagtcatt ttttatcacc aatccattgc cactgcgatt tatcagtaac tttttaaagg
     2281 tcgctttatt caccggagca atacccgccg gccggtaaaa caatttgatt ctgttttgca
     2341 tcgcaaactt cagtgcattc ttaccttctt gttttggact attcggtgga atatccagaa
     2401 cattaagata aaaaatactt tccttattac tgggtaattt attaggcatg attttaattt
     2461 ttatttgttg cccagaattg gccgctactt tcactactgg tggcgttaac atgaaaggaa
     2521 cctgaatctt ttctggcggc agagacgtgt cgccatcatc aatccacgcc tgcatcagcg
     2581 aagagcggtt tccctgattc atcagttgta ccatgacctc tttctgctct gccgggtaaa
     2641 ttatacgggt cccgaaaata accattccgg catgcacggg gaatatcata gaagagatga
     2701 gtaaagataa taaccctttc atcttaatgc ctcttatatt tatgctggcc gccatgaata
     2761 tggcggccat tttaacttac tgaaaaaaca agatgattat tttaaatatt taatcctggg
     2821 caacacgcat aaccaatgaa gagttaacag taccagatgc aacaccactg tcagtcaact
     2881 tcagatattt tgccttaaac ggtattgcat attatccatc agcagtttta taactttcaa
     2941 tattaaatgg tgcggaaata tccagtacgt cactgctatc tcccaaatag agatagtatc
     3001 caactgtgtc attactactg gatattgcct tatcattacc aagaattttc tcgttggtga
     3061 tcaacgaaag ttttacgcca tcaatagtct tcggacaagt aattttcagc atactgctgg
     3121 ttacaccaac cacgccatca taagttgaat atttcgcttt accatcaaat acagtggtgg
     3181 gtacagtcgg catattaata ctgactgaat caccgccagc gacaaatgta catggtgatt
     3241 cagcaatatt acctttaatg gttaaagttc cggtatctac ttcagcagcg aaaataccag
     3301 cactcataaa tacagaagat aagacagcga caacaataat tgaatgtttc atgatgcatt
     3361 ccttaataag tttattttgt tttgttaaga gtctgtatct cttggaaaca aatttctcac
     3421 atcaaaaaag ttgccgcaat gtaaacatat tgttttgttg atttaaaaca aattaactaa
     3481 tgcgatataa atacaacaaa taattcgcta taacaaacat ttttgaaact gattaattaa
     3541 cgtaaattac cgtaaataac attcatgagg tgaataaaaa agccaaaatc attcatagga
     3601 atgattttgg ctttttaaat atgcttaatg cttaaaacgg tgttggcaaa ttaatcgtag
     3661 cgatttaaca cgattttgcg tttgtcgtta ttacccacag ttaccacttc tgtcgtaacg
     3721 ccttttgcag tagaagaagc acgatagttg gtttcaactc gttgtaacaa ctgcccacaa
     3781 cgttgctgaa gatgattatt aacaacatcc atactgcatg taggggaact aattctgcca
     3841 ttgatggttg atgttgcagt ctctgaagag aacgcgggag aagccaatcc ataggcaaga
     3901 aaaataattc ctgac
//
LOCUS       DAJTMK010000265         3852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTMK010000265 DAJTMK010000000
VERSION     DAJTMK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.272"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            2..235
                     /locus_tag="OO135_004002"
     CDS             2..235
                     /locus_tag="OO135_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399623.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            527..682
                     /gene="hokD"
                     /locus_tag="OO135_004003"
     CDS             527..682
                     /gene="hokD"
                     /locus_tag="OO135_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5399624.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            899..1150
                     /locus_tag="OO135_004004"
     CDS             899..1150
                     /locus_tag="OO135_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399625.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQVLKEIGIKAGFSAFSEE"
     gene            1217..1495
                     /locus_tag="OO135_004005"
     CDS             1217..1495
                     /locus_tag="OO135_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399626.1"
                     /translation="MARNAKYYKHDNSTVLAHTHERYSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRLSRTYQQRIWR"
     gene            1497..2543
                     /locus_tag="OO135_004006"
     CDS             1497..2543
                     /locus_tag="OO135_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5399627.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTSAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEETVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            2556..2930
                     /locus_tag="OO135_004007"
     CDS             2556..2930
                     /locus_tag="OO135_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5399628.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLILSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMHEEQVKK"
     gene            2927..3748
                     /locus_tag="OO135_004008"
     CDS             2927..3748
                     /locus_tag="OO135_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5399629.1"
                     /translation="MKLEDLPKYYSPKSPGLTDVSASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQYPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
BASE COUNT          982 a          878 c         1087 g          905 t
ORIGIN      
        1 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
       61 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      121 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      181 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      241 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      301 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      361 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      421 aacattacgc gtccggcaag acgcttaaca ttaatctgag gccatatcta tgctctacac
      481 acgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
      541 ggcgatgcta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
      601 gaggaaagac ctctgcgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcac
      661 agcttacgaa cctgaggagt aagagacccg gcgagggaga aatccctcgc cacctctgat
      721 gtggcaggca ttctcaacgc acccgcactt aacccgcttc ggcgggtttt tgtttttatt
      781 ttcaacgcat ttgaagttct ggacggtgcc ggaatagaat caaaaatact taagtagcgc
      841 gcagggataa gagggatggt cccttaaagg ggagagctaa ttatccggaa ggattctgat
      901 gatgaacatc gaagaactgc gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt
      961 tgaaaatgga aatattgtta gtcattaccg cattgtgtgt ttgcaaaaaa atggggctgc
     1021 gttaattaat tttgtggatg cccgagtgac ggacggattt atcttgcgcg acggtgagtt
     1081 tgtcacttca ttacaggtat tgaaagagat cggaataaaa gctggctttt ctgctttttc
     1141 agaagaataa actcatctac aatcttgcgc ggggctgaac tcccgctgag taacaccgtg
     1201 ccaccggaga aaaccgatgg cacgcaacgc aaaatattac aaacatgata attcgaccgt
     1261 tcttgcccac acgcacgagc ggtattctca cgcatttaag tcagactggt accagcatcc
     1321 cccatgcact gaagaacagg ccgaatggct cattcagtgt taccgcaggc gcggatgcga
     1381 ggttaaaaaa gcccttagcc tcgactaccg tcactggata atctccgtca ggcttcctta
     1441 ctccgaacgc ccaccgcgtc tgtcccgcac ataccagcaa cgcatctgga gataacgtgc
     1501 gggtattact tcgacctgtt ctggtaccgg aactcgggct ggtgatcgtt aagccgggcc
     1561 gtgaatccat gccggtattc cacaataccc gggtactggt ggagccggaa ccgaaaagca
     1621 tgcgtaatct gccgtccggg gtcgttcctg ccgttcgcca gccgctggcg gaggataaat
     1681 cattactgcc atttttcagc gacgaacgag tgattcgtgc tgctggtggc gctggcgcat
     1741 tgtctgactg gttactgcgc catgttaaat cctgccagtg gccacacggc gattatcacc
     1801 acagtgaaac cgtcattcac cgttatggta ccagcgcaat ggtgttgtgc tggcactgcg
     1861 acaaccagct gcgcgaccag acctccgaat cactcgggca acttgctcac caaaacctgt
     1921 ctgcatggat gattgacgtc atacgccatg caatgaatgg ctcgcaggaa cgggaattat
     1981 cgctggctga attatcctgg tgggcggtcc gcaatcaggt ggcggacgcg ctaccggaag
     2041 cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat ccgctcaatg taccgtgaaa
     2101 gcgacatcgt accgggagag cagaccgcca ccagcatact gaagcagcgc acaaaaaatc
     2161 ttgcgccgct gcctcacgcc caccagcaaa acccgccaca ggaagagacg gtggtcagca
     2221 ttgccgttga tcctgagtct ccggaatctt tcatgaaacg acctaaacgt cgccgctggg
     2281 ttaacgagaa atacacacgc tgggtgaaga cacagccgtg tgcgtgttgt ggtaagccag
     2341 ccgacgatcc ccatcacctg attggtcatg gtcagggcgg aatggggaca aaatctcacg
     2401 atattttcac gctaccgctg tgtcgggagc atcacaacga gcttcatgcg gatcctctgg
     2461 cgttcgaaga aaagcatggt tctcaggttg atttaatttt tcgttttctt gatcacgcct
     2521 ttgcaactgg cgtgcttggg taaaagaggt gactgatgct catagatttg gttttacctt
     2581 acccgccgac ggtgaacact tactggcgac gccgtggcag cacatatttt atctcggagg
     2641 agggaaagcg ttatcgccgg gctgtggcgc ttattgttcg ccagcagcgg ctgaaattaa
     2701 tcctgtccgg aaggctggcg ataaaggtga ttgcagagcc accggataag cgtcgtcgcg
     2761 acctggacaa tatcctgaaa gcaccgctgg atgcgctgac gcatgcggga gtgttaatgg
     2821 acgatgagca gtttgatgaa atcaatatcg ttcgtggtca gccagtatct ggtggacgta
     2881 tgggggtgaa gatttacccc ataatgcatg aagagcaggt caaaaaatga aactggaaga
     2941 tttaccgaaa tactactccc caaaatcccc tggcctgacc gatgtatcgg cctcaacgtc
     3001 aaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc
     3061 tgagatgggg ttttctgcgt tcctggggaa aatgggcatc agtatgaatg acagggcgcg
     3121 ggcaacagaa ttactggcag attatgcact cagtcggtgc gatcgtgtgg cggcgttgag
     3181 aaaacttccg gcagaaataa aaccggtagt gatgcgcatt atggcttcgt acgcttttga
     3241 ggattatgcc cgcagcgcag cgagtaaaaa gcagtaccct tgttgctatg gggaaaaatt
     3301 tattgaaagc gtagttttta caaacaaggt ccagtatccg gatggtaagc cgccggtatg
     3361 ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga
     3421 ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa
     3481 ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc
     3541 tgttatcaga gactgccagc gttgtggtgg tcgtggctgt gaaagactac catcaacgga
     3601 ggcatttaat gccatatgca aagtgacgag tgctatcacg cttgatacgt ggaaaaaatc
     3661 agtgaaacgc ttttacgata cgttggtggt tcggtttgac attgaagagg catgggcgga
     3721 gcggcagtta aagagggtaa cgcgatagtg ttgttgattt ttcccgaatc tgtggtaaat
     3781 ttgctctaac gatgggcgtt ttatgcctga cgttagaaga ttttttacac cccgccgcct
     3841 ggcgggtttt tt
//
LOCUS       DAJTMK010000266         3835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTMK010000266 DAJTMK010000000
VERSION     DAJTMK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.273"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(327..1724)
                     /gene="ccp"
                     /locus_tag="OO135_004009"
     CDS             complement(327..1724)
                     /gene="ccp"
                     /locus_tag="OO135_004009"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5399630.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFPREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2128..3777
                     /locus_tag="OO135_004010"
     CDS             2128..3777
                     /locus_tag="OO135_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5399631.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          994 a          971 c          900 g          970 t
ORIGIN      
        1 tcgcgcattt cgtgcagcgg aaatcgtttt gactccgcta tagtagaaat ggcctttgcg
       61 ccaaaacgtg aatcgagtag ttctgagcgg aaatccgtta acagcttctg gtccatttcg
      121 aactccttaa atttatttga aggcaataaa aaagtaggat ttatccgcaa tggaagcaag
      181 gcattacagg cttaatttaa ataacaaaat cctaagcaga aaaacgactt tttataatat
      241 aaacatttaa catgataata ttaaaaacag actttattat cgtaataaaa atcgcccaaa
      301 cattcctgtt cgggcgatca aattaattat tgtttatcct gcatatacgg cgtgtacacc
      361 ccgttcagac tgtgcaggaa agctacgata tcatccacat cctcctgcgg cagctctttg
      421 cctacctgat agcgcagcat cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga
      481 tggaagtacg gtgccgttaa agcaacgtta cgtaaaccgg gtactttctg gcgcaattta
      541 tcacgctctt ctttagtcac attcatacga ccaatatccg ccgccgtaat ttccccaaag
      601 ttaaagtctt ttttcagccc caacggttca aaggaacgtc cgccgagaat aataccacca
      661 tgacaagttg cacatttatt atctttaaat aattgatagc cttttttctg ttgcgccgtc
      721 agcgcatttt catctccacg caaccattta tcaaatgggg aatccggcgt aattaatgtt
      781 ttctcaaatt cagcaatggc atcagtaata ttttcgccac tgaaaccttg cggataaact
      841 tcgaggaact gcgctttaag ctgcggatct ttttccagct tagcaataat ttcgtcccag
      901 gatttcgacg ccatttcaat cgggttcaac ggcggtccac ctgcctgatc ctgcaatgtt
      961 gccgcacgac catcccagaa ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg
     1021 ttaatcggcc caactgcgcc accaacacca atcgatgttt ttctgccatc gacgcccccc
     1081 gcattcaacg catggcaatg agcgcatgaa atggtgctat cagccgataa acggggatcg
     1141 tgatacagcg caaagcccaa cgccactttt tgcgcatcgg taggcagttt ttgcgggatg
     1201 ggctgcaccg gttcattgcg atgctccgga gcagtatcat tgctggcgta atattccgcg
     1261 cgctgttttg caatccaggc cagtatttcc gcccgctctt catcactcac cttacccgcc
     1321 cagtgtagcg cggtataacg cgttggtggc atagtttcat actgcatcac ccattcaatc
     1381 ttattcagat cgctttgcga aacgggttta tcagccagca gtgccgcacg cacggcctcg
     1441 aggttaaaag atttatatcc aagcttaatg tcgtaatcca tcaactgttt cgcgccagga
     1501 atataataat aggcgggtaa ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg
     1561 cggggaaagc ctaaaacctt attattttca ctgacagcag atgcctgaac atcggcctgt
     1621 ttactgcgtt tattatcgtg gtaccacaca taaccagata accctaaata gcaaatcgcg
     1681 acgccagcca ggccgatcgc ggtaatacgt gagaccattt tcattattct tccctcacgg
     1741 tggtgagtta ttgtgaccca gaaaattttc cagaatgcag caaacgtctc tgcgagacaa
     1801 agccatcata aaaaacaggg agatacgctt tttgatagga atcaatcaat tacatgaata
     1861 taaactatca ataagatagc ctgtgcctat tacccagatc aggaatggca tatcatgatt
     1921 gcggcgagag caggattgaa tgttctgaaa atgaaaaaca acaccaaatc cgtattttta
     1981 taaattccat tgatattagt gcgttatatt ttttgacaag ttaataaacg tgatccaccg
     2041 cacgctttgt cgcccaccag gcggagcgaa tgactaccct taaagaaaag ccgataatta
     2101 gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa ccctaatcca
     2161 gacgaactga tgatcgaagt cgatctctgc tatgaactgg acccgtatga attaaaactg
     2221 gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc cgcctctgat
     2281 gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt
     2341 ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct ggatatttta
     2401 atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaat
     2461 cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca aaattccctg
     2521 aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga tcacattccg
     2581 tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg ttttagcgaa
     2641 acctactatt gggattccta tttcaccatg ctggggctgg cggaaagtgg tcgggaagat
     2701 ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg tcacatcccc
     2761 aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc gctgatggtg
     2821 gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca cctgaaaatg
     2881 gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat
     2941 gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg cgacacgccg
     3001 cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg cccgcccaac
     3061 gaggtgtacc gcgatttacg agcgggggcg gcctccggtt gggattactc ttcccgttgg
     3121 ctgcgtgata ctggtcgtct ggcgagtatt cgtaccaccc agttcatccc catcgatctg
     3181 aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag
     3241 aaagagacag aagcactgtt ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt
     3301 tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg cgaacaactg
     3361 gcgctgtttt ccgctgccgc cattgtgcca ctctatgtcg gtatggcgaa ccatgaacag
     3421 gccgatcgtc tggcaaatgc cgtgcgcagt cggttactga cacctggcgg gattctggca
     3481 agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc gttacaatgg
     3541 atggcgattc agggatttaa aatgtacggc gatgaccttc tgggtgatga aatcgcgcga
     3601 agctggctga agacggtgaa tcagttctat ctggaacagc acaaactgat cgaaaaatac
     3661 catattgccg atggtgttcc acgcgaaggc ggcggtggcg agtatccgtt gcaggatggg
     3721 tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga accataatat
     3781 ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttca ggagg
//
LOCUS       DAJTMK010000267         3821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTMK010000267 DAJTMK010000000
VERSION     DAJTMK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.274"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..464)
                     /locus_tag="OO135_004011"
     CDS             complement(<1..464)
                     /locus_tag="OO135_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5399632.1"
                     /translation="MPVLYNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFID"
     gene            689..1609
                     /gene="lpxL"
                     /locus_tag="OO135_004012"
     CDS             689..1609
                     /gene="lpxL"
                     /locus_tag="OO135_004012"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5399633.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            1781..3005
                     /gene="mdtG"
                     /locus_tag="OO135_004013"
                     /pseudo
     CDS             1781..3005
                     /gene="mdtG"
                     /locus_tag="OO135_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            3088..3462
                     /locus_tag="OO135_004014"
     CDS             3088..3462
                     /locus_tag="OO135_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5399634.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(3463..3690)
                     /locus_tag="OO135_004015"
     CDS             complement(3463..3690)
                     /locus_tag="OO135_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5399635.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
BASE COUNT          928 a          974 c          976 g          943 t
ORIGIN      
        1 atgtcgataa atagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaga
       61 tactcaccaa cattgctggc atcaaagtga ggatcgtcaa taccgtcggc aacaatgcga
      121 tcacgtacct tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc
      181 aggcgtaaac cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca
      241 ttgctcgcag gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca
      301 aaaacattca gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg
      361 atgtggaaat acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg
      421 gcttttagcg cgtcgttgga aatgcggttg tataacactg gcatggtgta cggttcctgc
      481 gagatgggaa agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca
      541 cattttacgc tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg
      601 cccagtcttc agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg
      661 cccgaatagg ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact
      721 gcttcatccg cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca
      781 attgccctac ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt
      841 tatgaaacga cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag
      901 cgaacaagaa cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat
      961 ggaaaccggc atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat
     1021 cggcatggaa cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat
     1081 ccattttctg acactggagc tgggtgcgcg gcagtttggt atgcaggaac cggggattgg
     1141 cgtttaccgc ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg
     1201 ctcaaataaa tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa
     1261 aggcgaagtg gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt
     1321 cccgttattt gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat
     1381 gtccggcgca tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca
     1441 attgattatg ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc
     1501 gtggatgaac aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt
     1561 acaccgtcgc tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc
     1621 cggatgcttc agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa
     1681 agctctctgg attgcgcccc ccgggagtcg ggcgcataat tagtgtgctt atcttttctt
     1741 cttatgttca ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga
     1801 cacccctata aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg
     1861 tgccgccttc agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac
     1921 cgatcactcc gccctgaata tggtccggta ttgtcttcag cattacattt ttattttcgg
     1981 ccatcgcctc accgttttgg ggtggactcg ccgaccgtaa aggccgaaaa ctcatgctat
     2041 tacgctctgc cctcggcatg ggcatcgtga tggtgttgat ggggctggca caaaatatct
     2101 ggcagttttt gatcctgcgg gcgcttcttg ggttacttgg cggatttgtc cccaacgcta
     2161 atgctcttat cgccacacaa gtaccgcgta ataaaagcgg ctgggcgctg ggtacgctct
     2221 ccacaggcgg cgttggtggt gcgttgctcg gcccaatggc tggcggcctg ctcgccgata
     2281 gctacggctt acgtccggta ttctttatta ccgccagtgt gctcatactc tgctttttcg
     2341 tcaccctgtt ttgcatcaga gaaaaattcc agccggtcag caaaaaagag atgctgcaca
     2401 tgcgggaagt ggtgacatca cttaaaaacc cgaaactggt actcagcctg tttgtcacta
     2461 cgttaatcat ccaggtggcg acgggctcaa ttgcccccat tctgacgctg tatgtccgcg
     2521 aactggcggg taacgtcagt aacgtcgcct ttatcagtgg catgatcgcc tcggtgccag
     2581 gcgtggcggc tctgctaagt gcaccacgac tcggcaaact tggcgatcga atcggacccg
     2641 aaaagatcct gattacagcg ctgatctttt ctgtactgct gttgatccca atgtcttacg
     2701 ttcagacacc attgcaactt gggattttac gttttttgct cggtgccgcc gatggtgcac
     2761 tactccccgc cgtacagaca ttgttggttt acaactcgag caaccagatc gccgggcgta
     2821 tcttcggcta taaccaatcg tttcgtgata ttggcaacgt taccggacca ttgatgggag
     2881 cagcgatttc agcgaactac ggtttcagag cggtatttct cgtcaccgct ggcgtagtgt
     2941 tattcaacgc agtctattca tggaacagtc tacgtcgtcg tcgaataccc caggtatcga
     3001 actgattttt cgcctttcat acttgcaaaa gcggagaatc agctatcctt ttccctgaaa
     3061 cctcatcaac tcaaagggag aatcgtgatg accatgtacg caacgcttga agaagccatt
     3121 gacgctgcac gcgaagaatt tcttgcagac aaccccggca tcgacgccga agatgcgaat
     3181 gtgcaacagt tcaatgccca aaaatacgtt ttgcaggacg gcgacatcat gtggcaagtt
     3241 gagttttttg ccgacgaagg ggaagaaggt gaatgtttac ctatgcttag cggtgaagcc
     3301 gcgcaaagtg tttttgatgg cgactatgat gagatagaga tacgccagga gtggcaggaa
     3361 gagaatacat tacatgaatg ggacgagggg gaatttcagc ttgagccacc gctggatacc
     3421 gaggaaggac gcgcagcagc tgatgagtgg gatgaacgtt aatcactcat acgggccatg
     3481 atgaatgtcg atcggcagca gtaaagtatc aaaaaccagg gagaatggca gatcaaggat
     3541 tgtgacataa cgccaggcgg agtcacgcac atcccattgc acaccgggat aatattggtt
     3601 gccatgcccc tgccccggta tagtgcgact aataatgctg ccacagccac tcagcaaggt
     3661 caccatgatg ctcaccacaa ttaaacgcat ttgatacctc ttccgtaaaa aaataccggc
     3721 ataacgccgt aatgccagtc agttaagcaa ctgactggct ctttttcggg gctgtggggt
     3781 atttccaggg cctctccttt accactctcg ggaaggccct t
//
LOCUS       DAJTMK010000268         3764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTMK010000268 DAJTMK010000000
VERSION     DAJTMK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.275"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..587
                     /locus_tag="OO135_004016"
                     /pseudo
     CDS             1..587
                     /locus_tag="OO135_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(615..704)
                     /locus_tag="OO135_004017"
     tRNA            complement(615..704)
                     /locus_tag="OO135_004017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(668..670),aa:Ser,seq:cga)
     gene            798..1595
                     /gene="mtfA"
                     /locus_tag="OO135_004018"
     CDS             798..1595
                     /gene="mtfA"
                     /locus_tag="OO135_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5399636.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1696..1771
                     /locus_tag="OO135_004019"
     tRNA            1696..1771
                     /locus_tag="OO135_004019"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1729..1731,aa:Asn,seq:gtt)
     gene            2085..3764
                     /locus_tag="OO135_004020"
                     /pseudo
     CDS             2085..3764
                     /locus_tag="OO135_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT         1017 a          890 c          898 g          959 t
ORIGIN      
        1 tgtgcaggtg taagtaaatt caaggccaaa accgcgaacg atatgtaacc gacaaagaat
       61 accaggcagt attaagcgtt gcacctcttc ctgtttttat cgcaatggaa attgcctatc
      121 tgtgtgcagc gagggtttcc gatgtgttat cgctgaaatg ggagcagatt ggaaacgacg
      181 ggatctttat ccagcaaggg aaaacaggaa aaaaacagat aaaagcatgg agtccacgat
      241 tacaggcggc gatcgaaaaa gcaaaacagt taccaacatc cgcctatgta atcagcaatc
      301 aatacggcaa ccgatatatg tacaaaggct ttaacgaaat gtgggtagaa gcaagaaatc
      361 gcgcaggcaa aatttcaggt attttaaccg acttcacctt tcatgatctg aaggcgaaag
      421 gaatttcaga ctatgaagga agcagtcggg ataagcaact tttctctggt cacaaaaccg
      481 aggggcaagt gctaatctat gacaggaagg ttaaagtttc accgacactt gatgtcccgt
      541 tacctgaaaa tattccaagt aattccacgt gtgatttttg tcactgactt aatgatgtat
      601 aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact
      661 ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca
      721 tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg
      781 agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa
      841 ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg acggaacagg
      901 aacaaagcaa attagtcact cttgccgaac gttttttaca gcaaaagcgg cttgttcctt
      961 tacagggctt tgaactggat tcattaagaa gctgccggat agcacttcta ttttgcctac
     1021 ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg
     1081 cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta
     1141 ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac
     1201 aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg
     1261 acacccgtaa cggcgatcgc gccagcggag ttccctttat tccgttgcgt gaggttgctg
     1321 gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattggttg
     1381 gcgagaatgc ggcgagcatt gatgcttatg cagccagcga tcctgctgaa tgttttgccg
     1441 tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt
     1501 ggcaacgttt ctgccaattt tatcaacaag atcctttgca gagactgcat cacgctaatg
     1561 atacagactc gttttcggcg acgaatgttc attaattaac aactttgcag attaattaac
     1621 caattgaaat gacttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg
     1681 ccccgttcac acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
     1741 gtcactggtt cgagtccagt cagaggagcc aaattctaaa aattcgcttt tttagcgcaa
     1801 tgtcactgac cttagttgaa cattgctttt taacggatag cgggttttta acatcttaag
     1861 cgccctcgac ctttatggtt gagggcgttt tgctatgaac gccatcacca ttttcccctc
     1921 gattataaaa cttgagttat tcagtagtct cccctcttgc aactcacacc caaaactgcc
     1981 taacgaaaag ttattaattt tcaatcatat tgctatcagt atttacattt tttcgctgtg
     2041 ctagaaaggg cgcatttatg ttagctcgtt cagggaaggt aagcatggct acgaagaaga
     2101 gaagtggaga agaaataaat gaccgacaaa tattatgcgg gatgggaatt aaactacgcc
     2161 gcttaactgc gggtatctgt ctgataactc aacttgcgtt ccctatggct gcggcagcac
     2221 aaggtgtggt aaacgccgca acccaacaac cagttcctgc acaaattgcc attgcaaatg
     2281 ccaatacggt gccctacacc cttggagcgc tggaatcggc ccaaagcgtt gccgaacgtt
     2341 tcggtatttc ggtggctgag ttacgcaaac tcaaccagtt tcgtacgttt gctcgaggtt
     2401 ttaataatgt ccgccagggt gatgaactgg atgtcccggc acaagttagt gaaaaaaaat
     2461 taaccccgcc gccgggtaat agcagtgaca acctcgagca acagatagcc agtacttcac
     2521 agcaaatcgg gtctctgctc gccgaagata tgaacagcga gcaagcggca aatatggcgc
     2581 gtggatgggc ctcttctcag gcttcaggcg caatgacaga ctggttaagc cgcttcggta
     2641 ccgcaagaat cacgctgggc gtggatgaag attttagcct gaagaactcc cagttcgatt
     2701 ttctccatcc gtggtatgaa acgcctgata atctcttttt cagtcagcat actctccatc
     2761 gtactgacga gcgtacgcag attaacaacg gcttaggttg gcgtcatttc actcccacat
     2821 ggatgtcagg catcaacttc tttttcgacc acgatcttag ccgttaccac tcccgcgccg
     2881 gcattggcgc ggagtactgg cgcgactatc taaaattaag cagtaacggc tatttgcgac
     2941 tgaccaactg gcgcagcgca cctgaactgg acaacgatta tgaagcacgc ccggccaatg
     3001 gctgggatgt acgcgcagaa agctggctac ccgcctggcc gcaccttggc ggtaaactgg
     3061 tctatgaaca gtattatggc gatgaagtgg ccctgttcga taaagacgat cggcaaagta
     3121 atcctcatgc cataaccgct ggacttaact ataccccctt cccgctgatg accttcagcg
     3181 cggagcaacg ccaaggtaaa cagggcgaaa atgacacccg ttttgccgtc gattttacct
     3241 ggcaacctgg cagcgcaatg cagaaacagc ttgacccgaa tgaagtcgct gcacggcgta
     3301 gccttgcagg cagccgttat gatctggtgg atcgcaacaa caatatcgtt ctggaatatc
     3361 gcaaaaaaga actggttcgc ctgcccctga cagaccccgt gacagggaag tcaggagaag
     3421 tgaaatcact ggtttcgtcg ctacaaacca aatatgccct gaaaggctat aacgtcgaag
     3481 ccaccgcact ggaagctgcc ggtggcaaag tggtcacaac gggtaaagat attctggtta
     3541 ccctgccggc ttaccggttc accagtacgc cagaaaccga taacacctgg ccgattgaag
     3601 tcaccgctga agatgtcaaa ggcaatttgt tcgaatcgtg aacagagcat ggtagtcgtt
     3661 caggcaccta cgttaagcca gaaagattcc tcggtatcgt taagtaccca gacgttgagc
     3721 gcggattccc attcaaccgc cacactgact tttattgcgc atga
//
LOCUS       DAJTMK010000269         3745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTMK010000269 DAJTMK010000000
VERSION     DAJTMK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3745
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.276"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1615)
                     /locus_tag="OO135_004021"
     CDS             complement(<1..1615)
                     /locus_tag="OO135_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5399637.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGKACAFNDIIPADHCLHDVQDMSALNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQGEFDMSAATYAQQPDLFTAMLKQFRTDLSGFNAQCHGGSAAVV
                     PWICGDTTYYWKNTYGTQYDSVYGAYKNRESDNVFFVPFMTDGNGNNTPTNLPAEDPD
                     IADAGYYGAQSRSNGNWVSSNRPTHFSSWARRGIISDRLATAILNAVGRTSAFISGTA
                     PEIKPSPGGDTPSGPSDGDTSVRTVSLLPTAGEAAAQGWTITGGSVALEDGVFKVTKQ
                     SNKTWSLMHPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMT
                     GTGNPFLMSFFTQTTDGKLNLMHHKKAGNT"
     gene            1875..2210
                     /gene="iraM"
                     /locus_tag="OO135_004022"
     CDS             1875..2210
                     /gene="iraM"
                     /locus_tag="OO135_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5399638.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            2281..2493
                     /locus_tag="OO135_004023"
     CDS             2281..2493
                     /locus_tag="OO135_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399639.1"
                     /translation="MPLHGAGCLPVNSVPAPESAIIPYTYSLIAPPHRGIHHAKFFNK
                     LPAGQTIIASLNYEQRSILRENCFLQ"
     gene            complement(2959..3069)
                     /locus_tag="OO135_004024"
     CDS             complement(2959..3069)
                     /locus_tag="OO135_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR5399640.1"
                     /translation="MVDFSSRIMSVLSDDVLVAGVVVVAFPLLKKKASGD"
     gene            complement(3159..3235)
                     /locus_tag="OO135_004025"
     tRNA            complement(3159..3235)
                     /locus_tag="OO135_004025"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3199..3201),aa:Arg,seq:tcg)
     gene            complement(3243..3318)
                     /locus_tag="OO135_004026"
     tRNA            complement(3243..3318)
                     /locus_tag="OO135_004026"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3283..3285),aa:Ile,seq:cat)
     gene            complement(3359..>3745)
                     /locus_tag="OO135_004027"
     CDS             complement(3359..>3745)
                     /locus_tag="OO135_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR5399641.1"
                     /translation="GELEKPHHQLVDTYTSLNRQYVELQSEYKHLRRYFGVTAQVPYT
                     DVWTHKPVQFYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMDSGSTVKAALALGRR
                     AIGVELETGRFEQTVREVQDLVSQNG"
BASE COUNT          965 a         1019 c          863 g          898 t
ORIGIN      
        1 ttgtgtttcc ggctttcttg tgatgcatca gattcagttt gccgtctgtg gtctgggtga
       61 agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca ggtaacgcta
      121 catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg cctgacagtc
      181 gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca tccaccggat
      241 gcatcaggga ccaggtttta ttgctctgct tggtaacctt aaacacacca tcttccagcg
      301 caacactgcc gccggtgatg gtccagccct gcgcagcagc ctctccggct gtcggcagca
      361 gggagactgt acgaacggat gtgtcaccat cagacggccc cgatggcgtg tcgccgccgg
      421 gcgagggttt aatctccggt gcggtaccgc tgatgaaggc gctggttcga ccaactgcgt
      481 tcagaatagc ggttgccagg cgatccgaaa taatgcccct gcgcgcccat gaactgaaat
      541 gtgtcggacg atttgacgat acccaattac cattactacg ggattgcgcg ccgtaataac
      601 ctgcatcagc aatatccggg tcttctgccg gtaagttggt gggcgtgttg ttgccgttac
      661 cgtcggtcat gaacggcaca aagaaaacgt tgtcgctctc cctgtttttg tacgcgccgt
      721 agacggagtc atactgtgtg ccgtatgtgt ttttccagta atacgtcgtg tcgccacaaa
      781 tccacggtac aactgcagca ctgccgccat ggcactgcgc gttaaatccg gaaaggtcag
      841 tacggaactg cttcagcatg gccgtgaaca ggtccggttg ctgtgcgtag gtggcagcgc
      901 tcatgtcaaa ttcgccctgc atccagcaca ccgccagcaa cacatttttc gggttcttct
      961 gtaatgccgc tttggtgcgt gcgatcaggt cctgatataa cggtttaccc acaccccagc
     1021 gtgccgaatc ctggctggcc cccgtgtccg cactgaatgt cccctccgcg ccctgggtga
     1081 atgccgaacc accacgacag catggtacca gcaggatccc cgcgttattc gggatatacg
     1141 gaagcagttt tttggcaata tgtaagccct ggccgacaca gccgtactgc cctttgctca
     1201 ggtctgcctt cggatgattc agtgcgctca tatcctgaac atcatgcagg cagtggtctg
     1261 ccggaatgat gtcgttaaat gcgcatgctt taccaccggg agtcactgtg ttgcgacggg
     1321 ccagttgctt aatacgtggg tggggcgcat cgtaagaatc cggaagcgga agcccttcac
     1381 cgtaagccat ggcattggat tgcccggcca gtacgatgac gtagtaccac tccggctcag
     1441 ttgcaccact gacgaccaca tcaccttctg ctgcaatcgc ctgcatcagg gtataagggg
     1501 ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt gtcagctttt
     1561 ccgcaagatc tgacggcgac gccgccctga caacatcata gtgtttaaat gccatggttc
     1621 tttccaccat ctgaaaaatg attctttaaa atacctgaca tgtaatacag aaaaaaacaa
     1681 aaccatacct taaataaaaa cctcatcatc aagcagatat gcatggataa actacaagac
     1741 gagatataaa ccaccatgca tttaaataaa caataaacaa catcagaaaa ataattctgc
     1801 tctatggttt acaatcaaaa atatcattta tacttttcag aacatcacca gcaagacata
     1861 aacaaggaaa ctaaatgaag tggattgtga ttgatacagt tatccagcca tcatgcggaa
     1921 tatctttttc agtcatatgg agtaaaataa aattaataat ctggtatcaa tcggatgctt
     1981 tcttacctcc tgaaagtata tttacactga ctcacacagg catcatgctc aataacaaag
     2041 tgctacctgt aaccatttac aacgtagtgc cattcaataa aacattctgg aatttaatca
     2101 aaaacagcca ggaatgccct acaaatacag ataacgtatt gaatgaatgc tttaataacc
     2161 gttgcactct gcaaatatgt ccttatggac taaaacaaca aagtccataa ggagtttact
     2221 cacatctgac aaaatcaata taaacagccc ctccggagag gggctggaga gtggcgctat
     2281 gtgccattgc atggtgccgg gtgcctcccg gtgaattcag taccagcacc tgaatccgcg
     2341 attatcccat atacctactc gctgattgcc cctccgcaca gggggattca ccatgcgaaa
     2401 ttttttaaca aacttcccgc tggccagaca ataatcgcca gcctgaatta tgagcaacgt
     2461 agcattttac gggaaaactg ttttctgcag taaaaaggcc cgccggagcg agcctggaag
     2521 gatagcggtc atgtgatgcc ggtttcccgg taactcagca tcggtatctg agtcaacgtt
     2581 ttctctactg ggtcatttcc gatacgttcc gccttccggc agactttcat cacgtcagaa
     2641 aatatagcac cctgagtaac aggacagtac tcagaattca ggaaactgtg acacatcctg
     2701 cacagaaaag cccctccgga gaggggctga agtatagcct aatttctgtc tgtcgcatgg
     2761 tgccaggtgc ctcccggtaa attcagcctg tctactgaat ttgcatgttc tctggatcat
     2821 acactttgcc agatgccccg ccgctgaggg ggattcacca tgcgatgtaa tttttaacaa
     2881 attctccggc agccagacaa tcatcaagct gtggaattgt gaggtattta aaaatttcaa
     2941 cgggtaactg ataccctgct aatcgcctga tgctttcttt ttcagcaacg ggaaagcaac
     3001 aaccaccaca cccgccacca aaacatcatc agacagcacc gacattatcc ggctgctgaa
     3061 gtccaccatc accaccagaa acaacaggag tgcaaccaca gctgcttaga aggcaattgc
     3121 tctgtccggc tgagctaaca acgcagaata ccgataaatg gaccgccatc gagaactcga
     3181 accccgcgca accagcttcg aaggctggcg ctctatcccg atgagctaat ggcggtatgt
     3241 gatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
     3301 ccactgagct aaagggccgg gagccgcata ataacgacgc gtaattaatt ctgcaatatc
     3361 atccgttctg gctgactaaa tcctgtactt cccgaaccgt ctgctcaaaa cgtccggtct
     3421 ccagctcaac gccaatcgca cgacgcccga gcgccagtgc cgcttttacc gttgaacctg
     3481 agtccataaa aaaatctgca accaggtcac ccggacgact gcttgcgctg attatctgct
     3541 gcagcatttc tgccggtttt tcgcacggat gtttcccggg atagaactgc accggtttat
     3601 gtgtccacac atccgtgtac ggcacctgcg ccgtcacacc aaaataccgc cgcagatgct
     3661 tatattcact ctgcagctcc acatactgcc ggttcagtga cgtatacgta tccaccagct
     3721 ggtggtgggg cttttccagt tcacc
//
LOCUS       DAJTMK010000270         3663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTMK010000270 DAJTMK010000000
VERSION     DAJTMK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.277"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..3282
                     /locus_tag="OO135_004028"
     CDS             <1..3282
                     /locus_tag="OO135_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5399642.1"
                     /translation="TAVWRAGEQEQTPPEGFESSGSETGLGVEVTKAKPVTRTITSAN
                     IDRLRVTFGVQSLVQTTSKGDRNPSSVRILIQLQRNGRWVTEKDVTINGKTTSQFLAS
                     VILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDA
                     EQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTH
                     PRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRKAWDVLS
                     DFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTA
                     VEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLWVIKTGL
                     LETQTVDFTLGSQGLRHTPGYIIEICDNDYAGTMTGGRVLSIDAATRTLTLDREVTLP
                     ETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFR
                     CVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLTVEVSTA
                     DGQYLAQAKWDTPRVVKGGRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTL
                     TVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTVQFEFWF
                     SEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGR
                     ASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEITQTVSK
                     TLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSN
                     ILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPE
                     GRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSR
                     APERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEI
                     YNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKATA
                     GISIS"
     gene            3350..>3663
                     /locus_tag="OO135_004029"
     CDS             3350..>3663
                     /locus_tag="OO135_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5399643.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSV"
BASE COUNT          849 a          965 c         1115 g          734 t
ORIGIN      
        1 accgcggtct ggcgtgccgg ggagcaggag cagacaccac cggaaggctt tgagtcctcc
       61 ggctctgaaa ccggactggg cgtggaagtg acgaaggcaa aaccggtgac gcgcaccatt
      121 acgtccgcga acattgaccg cctgcgggtt accttcgggg tgcagtcact ggtgcagacc
      181 acgtcaaagg gcgaccgtaa tccttcctct gtccggattc tgattcagtt acagcgtaat
      241 ggccgctggg tgacggaaaa ggacgtcacc attaacggca agaccacctc acagttcctg
      301 gcctcggtga ttctggataa tctgcctccc cggcccttta acatccggat ggtcagggag
      361 acggcggaca gcaccacgga ccagctgcag aataagacgc tgtggtcgtc atacaccgaa
      421 atcatcgatg tgaaacagtg ctacccgaac acggccattg tggggctgca ggtggatgcg
      481 gagcagttcg gcggccagca gatgacggtg aactaccata tccgcggtcg catcatccag
      541 gtgccgtcaa actatgaccc ggaaaaacgc acgtacagtg gtatctggga cggcagtctg
      601 aaaccggcat acagcaacaa cccggcctgg tgcctgtggg acatgctgac tcacccgcgc
      661 tacggcatgg gaaaacgtct gggggcggcg gatgtggaca agtgggcgct gtatgccatc
      721 gggcagtact gcgaccagac ggtcccggat ggtttcgggg ggaccgagcc gcggatgacc
      781 tttaatgcgt acctggcaca acagcgtaag gcgtgggacg ttctcagtga tttctgctct
      841 gcgatgcgct gtatgccggt atggaacggt cagacgctga cgttcgttca ggaccgcccg
      901 tcggatgtgg tgtggccgta caccaacagc gatgtggtgg tggatgataa cggcgtggga
      961 ttccgctaca gcttcagtgc cctgaaggac cggcacacgg cggtggaggt gaattacacc
     1021 gacccgcaga acggctggca gacctccacg gaactggtgg aagacccgga agccatactg
     1081 cgctacggac gcaacctgct gaagatggat gcgtttggct gtacctgccg cggtcaggct
     1141 caccgtgcag gtctgtgggt gataaagacc ggactgctgg aaacgcagac ggtggacttc
     1201 acgctcgggt cacaggggct gcgtcacaca ccgggttaca ttattgaaat ctgtgataac
     1261 gactatgccg ggactatgac cggcggacgt gtcctgtcca ttgatgctgc cacccgcacc
     1321 ctgacgctgg accgtgaggt taccctgccg gagacaggta catcggcggt gaacctgatt
     1381 aacggcagcg gtaagccggt gagtgtggac atcaccgcac accccgcgcc ggaccggata
     1441 caggtcagta ccctgcctga tggtgtggag acatacgggg tgtggggact ctccctgccg
     1501 tcactgcgcc gtcgcctgtt ccgctgtgtc tccgtccggg aaaacacgga cggcaccttt
     1561 gccatcacgg cggtgcagca cgtaccggaa aaagaagcca tcgtggataa cggtgcccgc
     1621 tttgagccgc agtcaggctc cctgaacagc gtcatcccac cggcagtgca gcacctgacg
     1681 gtggaggtga gcacagctga cggccagtat ctggcacagg cgaaatggga cacgccgcgg
     1741 gtggtgaagg gggggcgctt cagtctgcga ctgaccagcg gaagcggaga agacagccgt
     1801 ctggtgacca ccgctatcac tgcggataca gagcatcgtt tcagtggtct gccgctcggg
     1861 gaatacaccc tgacagtcag ggcaattaac agttatggcc agcagggcga accggccacc
     1921 accaccttcc ggattaacgc gccagcaaaa cccgccacca ttgaactgac gccggggtat
     1981 tttcagataa cagcggtacc gcgtcttgcg gtgtatgacc cgacggtaca gtttgagttc
     2041 tggttttcgg aggcaaaaat cgcagacaca tctcaggtgg aaacctctgc ccgttatctg
     2101 gggaccggca gtcagtggag tgtatccggc ccgcacatta agcccgggaa ggatttctgg
     2161 ttttacgtgc gcagcgtcaa cctggtgggg aaatctgctt ttgtggaggc cagtggacgg
     2221 gccagcaatg atgcagaagg gtatctgggg ctgtttcggg aaaaaatagg aaaactgcat
     2281 ctggctcagg ggctgtggga gctgatagac aacagccagc ttgcggatga gatggcggag
     2341 atgaagacca ccatcaccga aacccgcaat gaaatcacac agacggtcag taaaacgctg
     2401 gaggaccaga gcgccaccat acagcagata cagcgcgtgc agaaggacac aaatgatgac
     2461 cttgctgcac tttacatgct gaaggtacag aaaacaaaaa atggcatacc ctatgttgcc
     2521 ggtattggag cggggattga ggatactgat ggccagcccc tgagcaacat actgctgctg
     2581 gctgaccgta ttgcgatgat taacccggag gacggcaaca ccacgccgtt atttgtggcg
     2641 caggggaatc agttgttcat gaacgatgtg ttcctgaagc ggctgtttgc ggtgagtatc
     2701 acctcgtccg gcaatccccc gacgttttcc ctgacgccgg agggcaggct gaccgcaaga
     2761 aatgctgata tcagcggtaa cgtgaatgcg aactccggga cgctcaacaa cgtcacgatt
     2821 aacgagaact gccgggttct gggaaaactg tccgcgaacc agattgaagg cgatctcgtt
     2881 aaaacagtgg gtaaggcttt tccgcgggac tcccgggcac cggagcggtg gccatcaggg
     2941 accgttaccg tcaggattta tgacgatcag ccttttgacc ggcaaattgt tattccggcg
     3001 gtggcattca gcggcgctaa gcatgagaga gagcatactg atatttactc ctcatgccgt
     3061 ctgatagtgc ggaaaaacgg tgctgaaatt tataaccgta ccgcgctgga taatacgctg
     3121 atttacagtg gcgttattga tatgcctgct ggtcacggtc acatgacgct ggagttttcg
     3181 gtatcagcat ggctggtgaa taactggtat cccacagcaa gtatcagcga tttgctggtt
     3241 gtggtgatga agaaagccac cgcaggcatc agtatcagct gaattttata acccatatac
     3301 gggcgccaga aatggcgcct tttttattgc agaaaagcga gaggtaatta tgcgtaaagt
     3361 ttgtgcagcc attttgtccg cagccatctg tctgtccgta tccggtgcgc ctgcatgggc
     3421 gtctgaacat cagtccacac tgagcgcggg atatcttcat gcccgtacga acgctcccgg
     3481 cagcgataat ctgaacggga ttaacgtgaa ataccgttat gagtttacgg acgcgctggg
     3541 gctgattacg tccttcagtt atgccaatgc tgaagatgag aaaaaaacgc actacagcga
     3601 tacccgctgg catgaagatt ccgttcgtaa ccgctggttc agcgtaatgg cggggccgtc
     3661 tgt
//
LOCUS       DAJTMK010000271         3625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTMK010000271 DAJTMK010000000
VERSION     DAJTMK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.278"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..357)
                     /locus_tag="OO135_004030"
     CDS             complement(<1..357)
                     /locus_tag="OO135_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5399644.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGG"
     gene            complement(700..2154)
                     /gene="amn"
                     /locus_tag="OO135_004031"
     CDS             complement(700..2154)
                     /gene="amn"
                     /locus_tag="OO135_004031"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5399645.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGFFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(2256..3572)
                     /gene="shiA"
                     /locus_tag="OO135_004032"
                     /pseudo
     CDS             complement(2256..3572)
                     /gene="shiA"
                     /locus_tag="OO135_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
BASE COUNT         1021 a          860 c          840 g          904 t
ORIGIN      
        1 gccgcctttt ttattgaaaa ttgtgcgaac gttagcaatc gtacggttaa catttgaagt
       61 caatgtctcg gcgataatca ttgagccatt aggaccaaag ccttcataac gtccctgcac
      121 gaacgtttca tctccgccgc ctttggcttt atcaattgct ttatcaataa cgtgttttgg
      181 aacttgtgcc tgctttgcac gttcaataac gaattttaaa gatgtgttta attctggatc
      241 gggttcacct tgtttagcag cagcatagat ttctacaccg aattttgcat aaattttaga
      301 cgttgcaccg tctttagccg tttttttagc aacaatattg gcccatttac gtcccactgg
      361 aatgatctcc tcgaaaattt atttacatta ctggtaaatc gttaacatgt gttaattcgg
      421 catattatat aataacagga aaaatctcgt ctattagaaa taaaaatatt ctaaagtcat
      481 aatcttatat ttaattttat ataaacttat attgttggtg ggatttttta taattgtgtt
      541 ttgaaagcat acttgctttt aatatgtgga aatcattaag acgataataa aactcgtatc
      601 cgcccaagga agcaaacagc ccgcgtttat tgcgggctat ttgccagatg tcttacttaa
      661 tccttcacat ctatttttga taagaaggtt cagaacttat tatcggaacg gcggctcatt
      721 aaaggttcgt aatttacgtg aatgcagtcg gtcgccttct gcgcgcagca aatcgatcgc
      781 ccgaatgcca atttgcaggt gttcggaaat agcaccttca taaaaacggt tagcctggcc
      841 gggaagttta atctcgccat gcaacggttt atctgaaaca cacagtagtg tcccgtatgg
      901 cacgcggaaa cgatatcctt gcgcggcaat ggttgcactt tccatatcaa ttgctacggc
      961 ccggcttaag ttaaaacgaa gtgcagaagc tgagtaacgt aattcccagt tcctgtcatc
     1021 agtggttact acagtgccag tacgtagccg ctgtttaact tcctcaccgg gcctgccgct
     1081 caccagcttg gtggcgtcat aaagcgcacg ttgcacttca gcaatgctcg gaataggaat
     1141 atcgggcggc agaaccgcat caagaacgtg gtcatcgcgt aaataagcgt gtgcaagtac
     1201 ataatcgcca atggcctgac tttcacgtaa tccgccacag tgaccaatca tcaaccagac
     1261 atccgggcgt agtactgcca gatgatcgca gatggtttta gcatttgacg gtcccacgcc
     1321 aatattcacc agagtaatac cctgaccatc ggcggtaatt aaatgccacg cgggcatctg
     1381 gtgttttttc catgcaaggt cggaaatggc ttcttctggc gcttcggttt cggcggtgat
     1441 ccagttcccg ccagcacaag aaagggcaat gtagggacta tcaggatcga ggatctggct
     1501 gcatccccaa cgaacgaatt catccacata acgtgtgtag ttggtaaaca agacgaacgg
     1561 ctgaaaatgt tcaactggcg taccggtata atggcgcaac cgcgcgaggg aaaaatcgac
     1621 gcggcgcgca tcaaaatgcg atagcgggga gaattcagtt gggtgataaa tgccgtctgc
     1681 agtttcatcg ccaatttgcg ccagttctgt tgtagggaaa tagcgagtta acccagcgct
     1741 cattgagcga tcaagtgtca attcagagcc atcgatgaca taaggataag ggatttcatg
     1801 ctgcgagggt tgcaccgaga tatgcgcacc ataatcctga tacagcaacg taagttgttc
     1861 attaagatac gaacgaaaga gagtagggcg agtaatcgtg gtggtgtagc tgcctgcgtg
     1921 agtaaaacga ccaaatgcgc gcgttttggg gggattggtt gtgctaccgt cccaggttac
     1981 ggtcagtgat ggatagacaa aaaaaccttg tttgcgggcg ttttcatcag gtaattcgcc
     2041 acttgtaata tagttgccaa tggcgttgcg taatgcgact acagattgct catacagcgc
     2101 gtcgagttta tccagtgcct gagctggggt cagaccggag cccttattat tcatgtgtgt
     2161 ctcctgttcc atacaattac cgcacatccg atagtatgtc acagaaatgt gaaacaaaag
     2221 tgtaaggcga agatcggatg atcttcgcca ggctatcaag cgcgttgact gtctttcatc
     2281 aacaaagcgg ttattgcaga aatcagacat ccagccagca gatacatggc gacgctatgc
     2341 cagttcccgg caaagtaagt gatgagtgca gcggcaataa aaggtgtaaa tcctccgcca
     2401 accacactgg caacctgata accgactcca gcgccactat agcgataact ggcaccaaac
     2461 atttcggtaa acatcggttg ttgcacacac accaccatgt catgcgcaat gtttgccagc
     2521 attatggaga agaaaactat ccagaaaata gattgtgctt caagcgccat aaagaaagga
     2581 aatgcgctca acgttccgat taacgcacct gtgatataaa ccctgcggcg accaaaacga
     2641 tcggcaagcc aggcaaaaca gggaattgtc aggcagctta atccacctac cagcaaacca
     2701 atattaagga aaagttcgcg cggtaacccc atattctagg ttgaataatt aagtgcaaag
     2761 gcggtaacga tgtacatcgt cagcaactcg cacagccgta gcgcaataat cttcaggaaa
     2821 gcacctggat gtcgtaacag cgcttcgata accgggatgc gttttttcgc ggcagcttga
     2881 ttatgttgct gttgttcgaa ttccgcggac tcctccatgc cattgcgcac ccacaatgct
     2941 cccagtacca gtacgatgct aaacaggaaa ggaatacgcc agccccaact taaaaactgt
     3001 tcgtcagtcg tcatcatact gatcaatgaa accagtccgg ttgaaagcag taaacctaca
     3061 ccgtagccaa cttgtacacc gctactgtaa aaggcttttt tatttttcgg tgcactttca
     3121 acggaaagca acgccgcgcc tccccattcg ccgccgacgg caaatccctg aatggcacgc
     3181 agcgtcacca gtaaaatagg tgcccaccac ccaatggtcg agaatgaagg aagaatacca
     3241 atcaaggctg tcgcgatgcc catcatccag acagtcagca ttaacatgcg cttacgtccc
     3301 agtcggtcgc caaagtgacc gaaaatgaca ccgccgagcg gacggaaaag gaatccgacg
     3361 ccaaaggtag caaatgcggc gagcgttccc atcgccgggc ttacttgcgg gaaaaactcg
     3421 cgattaaaca ccagtgcggc ggtgatgcca tagagtaaaa aatcatacca gtcgacgacg
     3481 gcaccagcga aactgcctaa cgcagctcgt cgggcgcgac ttaacgaaac cgtcctttca
     3541 tcgggacgag tggagatgag cgtggagtcc ataatgatcc tgtttgtact gtctttttat
     3601 aattagtatg gaaaaggtaa ctcac
//
LOCUS       DAJTMK010000272         3613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTMK010000272 DAJTMK010000000
VERSION     DAJTMK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.279"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1046)
                     /locus_tag="OO135_004033"
     CDS             complement(<1..1046)
                     /locus_tag="OO135_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5399646.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OO135_004034"
     CDS             1537..1647
                     /locus_tag="OO135_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5399647.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OO135_004035"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OO135_004035"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5399648.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..>3613)
                     /locus_tag="OO135_004036"
     CDS             complement(2963..>3613)
                     /locus_tag="OO135_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5399649.1"
                     /translation="TAVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDF
                     GINMNNVTNSSIDFTPLVNEPFVLACRRDHPLAKKQLVEWQELVGCKMIGVRSSSGNR
                     LLIEQQLADKPWKLDWFYEVHHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLV
                     EPVIRRTLGIIRRKDAVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          955 a          739 c          873 g         1046 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt tttgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgattaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcacctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc ttcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgatgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgcaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggcc agcacgaacg
     3421 gctcgttgac cagtggggta aaatcaatgg atgaatttgt gacgttgttc atgtttatgc
     3481 caaagtctga ttcgttacac agcaccgatt ccatgcaatt attcgtgcct tgttccagaa
     3541 tccgcacttt aatattggga tacagctcat taaatttacc gattgccagc ggtaaaaaat
     3601 aaaatactgc ggt
//
LOCUS       DAJTMK010000273         3591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTMK010000273 DAJTMK010000000
VERSION     DAJTMK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.280"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            175..474
                     /locus_tag="OO135_004037"
     CDS             175..474
                     /locus_tag="OO135_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5399650.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            569..1480
                     /gene="glyQ"
                     /locus_tag="OO135_004038"
     CDS             569..1480
                     /gene="glyQ"
                     /locus_tag="OO135_004038"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5399651.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            1490..3559
                     /gene="glyS"
                     /locus_tag="OO135_004039"
     CDS             1490..3559
                     /gene="glyS"
                     /locus_tag="OO135_004039"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5399652.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILEKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
BASE COUNT          853 a          935 c          991 g          812 t
ORIGIN      
        1 ctgtgtctct gtatctggtt aaaaaaagtc tgaccgataa atcatttggt tggcgcactg
       61 gaataatctg agttttatca ctacagctta tagaggctta aggaaattcg taagatatca
      121 gccactatac cgatataaat aataagactc acctgcaaac cagacggtaa tttaatgatg
      181 atgaacgctt tctttccggc aatggcgctt atggtgctag tgggttgttc tacaccgtca
      241 cccgtgcaga aagcacaacg ggtaaaggtt gatcctctgc gttcgttgaa tatggaagcg
      301 ttatgcaagg atcaggcggc aaaacgttat aacaccggcg agcaaaaaat cgacgtcacc
      361 gccttcgaac agttccaggg aagctatgaa atgcgcggtt ataccttccg taaagagcag
      421 tttgtctgtt cttttgacgc agatggccat tttttgcatc tttccatgcg ttaagccctg
      481 ctttttcccg tttcgtactg tatatcttcc atccagcggg tatactgatc ccttccttta
      541 aatccacacg tatccagcac gaaataatat gcaaaagttt gataccagga ccttccaggg
      601 cttgatcctg accttacagg attactgggc tcgccagggc tgcaccattg ttcaaccatt
      661 ggacatggaa gtcggcgcgg gaacctctca cccaatgacc tgtctgcgcg cgctggggcc
      721 agaaccgatg gcggctgctt atgttcagcc ttctcgtcgc ccgaccgatg gtcgctacgg
      781 cgaaaacccc aaccgtttac agcactacta tcagttccag gtggtcatta agccatcgcc
      841 ggacaatatt caggagctgt acctcggttc tctgaaagag ctgggcatgg acccgactat
      901 tcacgacatc cgtttcgtgg aagataactg ggaaaacccg actctgggtg cctggggact
      961 gggctgggaa gtgtggctaa acggcatgga agtgacgcag ttcacttact tccagcaggt
     1021 tggtggtctg gagtgtaaac cggttaccgg cgagatcacc tacggtctgg aacgtctggc
     1081 catgtacatt cagggcgtag acagcgttta cgacctggtc tggagcgacg gcccgctggg
     1141 taaaaccacc tacggcgacg tgttccatca gaacgaagtg gagcagtcca cttacaactt
     1201 cgaatacgcg gatgtggact tcctgttcac ctgctttgag cagtacgaga aagaagcgca
     1261 gcagctgctg gcgctggaaa atccgctgcc gctgccagcc tacgagcgta ttctgaaagc
     1321 cgcccacagc ttcaacctgc tggatgcgcg taaagccatc tccgtcaccg agcgtcagcg
     1381 ctacattctg cgcattcgca ccctgaccaa agcagtggca gaagcatact acgcttcccg
     1441 tgaagccctc ggcttcccga tgtgcaacaa agataagtaa gaggcggcta tgtctgagaa
     1501 aacttttctg gtggaaatcg gcactgaaga gctgccacca aaagcactgc gcagcctggc
     1561 tgagtccttt gctgcgaatt ttactgtgga gctggataac gctggcctcg cacacggcac
     1621 cgttcaatgg tttgctgctc cgcgtcgtct ggcgctgaaa gtagctaacc tggcggaagc
     1681 gcaaccggat cgtgaaatcg aaaaacgcgg cccggcgatt gcccaggcgt tcgacgctga
     1741 aggcaaaccg agcaaagcgg cagaaggttg ggcgcgtggt tgcggtatta ccgttgacca
     1801 ggctgagcgt ctgactaccg ataaaggcga atggctgctg tatcgcgccc atgtgaaggg
     1861 cgaaagcacc gaagcactgc tgccgaatat ggttgcgact tctctggcga aactgccgat
     1921 cccgaaactg atgcgttggg gcgcaagcga cgtgcacttc gtgcgtccgg tgcacaccgt
     1981 aaccctgctg ctgggcgaca aagtcattcc ggcaactatt ctgggcattc agtccgatcg
     2041 cgtgattcgc ggccaccgct ttatgggcga accggaattc accatcgaca acgccgatca
     2101 gtatccggaa attctgcgtg agcgcgggaa agtcatcgcc gattacgaag aacgtaaagc
     2161 gaagattaaa gccgatgccg aagaagcagc gcgtaagatt ggcggtaacg ctgacttaag
     2221 cgaaagcctg ctggaagaag tggcttcgct ggtggagtgg ccggtcgttc tgaccgcaaa
     2281 attcgaagag aaattcctcg cggtgccggc tgaagccctg gtttacacca tgaaaggtga
     2341 ccagaaatac ttcccggtgt atgcgaacga tggcaaactg ctgccgaact tcatcttcgt
     2401 tgccaacatc gaatcgaaag atccgcagca aattatctct ggtaacgaga aagtcgttcg
     2461 tccacgtctg gcggatgccg agttcttctt caataccgac cgtaaaaaac gtctggaaga
     2521 taacctgccg cgcctgcaaa ccgtgctgtt ccagcaacag ctgggtacgc tgcgtgacaa
     2581 aactgaccgc atccaggcgc tggctggctg gattgctgaa cagattggcg ctgacgttaa
     2641 ccacgcaacc cgtgcgggcc tgctgtctaa gtgtgacctg atgaccaaca tggtcttcga
     2701 gttcaccgac acccaaggcg ttatggggat gcactacgcg cgtcacgatg gcgaagcgga
     2761 agacgtagcc gtcgcgctga atgagcagta tcagccgcgc tttgccggtg atgacctgcc
     2821 gtccaacccg gtagcttgtg cgctggcgat tgctgataag atggatactc tggcgggtat
     2881 cttcggtatc ggtcagcatc cgaaaggcga caaagacccg tttgcgctgc gtcgtgccgc
     2941 acttggcgtg ctgcgaatta tcgttgagaa gaacctcaac cttgatctgc aaacgctgac
     3001 cgaagaagca gtgcgtctgt atggcgataa gctgactaac gccaacgtgg ttgatgatgt
     3061 tatcgacttt atgctcggtc gcttccgcgc ctggtatcag gacgaaggtt acaccgttga
     3121 caccatccag gcggtactgg cgcgtcgtcc gactcgtccg gctgatttcg atgcccgtat
     3181 gaaagcggta tcgcatttcc gtaccctgga tgcagctgct gcactggcgg cggcgaacaa
     3241 acgtgtatct aacattctgg agaaatctga cgaagtgctg agcgaccgcg tgaatgcctc
     3301 taccctgaaa gagccggaag aaattaaact ggcgatgcag gttgtggtgc tacgtgacaa
     3361 gctggagccg tactttgcgg aaggtcgtta ccaggatgcg ctggtcgaac tggcagagct
     3421 gcgtgagccg gttgatgctt tcttcgataa agtgatggtc atggttgatg acaaagaatt
     3481 gcgtctcaac cgtctgacca tgctggagaa actgcgcgaa ctgttcctgc gcgttgcgga
     3541 tatttcgctg ttgcagtaat aacgccgtta ttaaaaagcc tgccatctgg c
//
LOCUS       DAJTMK010000274         3580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTMK010000274 DAJTMK010000000
VERSION     DAJTMK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.281"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(294..989)
                     /locus_tag="OO135_004040"
     CDS             complement(294..989)
                     /locus_tag="OO135_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5399653.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(982..2409)
                     /locus_tag="OO135_004041"
     CDS             complement(982..2409)
                     /locus_tag="OO135_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5399654.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVVETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHLGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2420..3139)
                     /locus_tag="OO135_004042"
     CDS             complement(2420..3139)
                     /locus_tag="OO135_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5399655.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          922 a          977 c          740 g          941 t
ORIGIN      
        1 tgccttgtca aaaaaatggt agatgaatac caacaaaact ctgggcagta ataaacaaca
       61 ttctgataga gagtgcctgt ataaagctga agtgacaatg accgggaagg gaacccgttt
      121 tcaggaatgg atgcctgtct ttttcactgt aaatatccta gcattactaa tgccaggata
      181 tttacagaag agcagcataa ccatgacgga acggggttaa gtcaggttgt ccttaacgct
      241 attttcaacc tcgtatgcct tcttcaggtt tatgttcaga tctcatatct ctctcagcaa
      301 tcctcaataa tcagatacac cgctttcacc gggccatgaa ccccgacgac tttgataagc
      361 tcaatatccg ccgttgaact agggccgcta atgatgttaa tgcaggacgg cattcgttca
      421 ccggcctgcg ctttctgatg caatttttct gcgagttgcg ctacacgcgg caggattgtg
      481 cttttacgca ggataaaaag agaagattcc gggagcaggc tcaatgaacg cccgcgctcg
      541 gcggcggaaa aaagaaccac gcctcccgat tcggttaaac catattcagc atacacaaca
      601 cccactttag cctgctctgc ctgcgagata ttctcggcac ctttcgccgg atcccaaaca
      661 acggcattgc attcctgctg caaacgttca ctaatcccca attcctccag cctcgtgtca
      721 ccgctaatca cgaccgactg atctcccagc tctttacaca gacgtattgc agcttctgcc
      781 gccttcgcct cgctggtcag ctcacagcgc gtcaacataa catcgctggc aaactgaata
      841 aacgcgtcac agcgctgctg ttggttaagt tgggtaagcc gctcgttagc atagttgtta
      901 agcggcgcat cttctgcttg cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg
      961 ttattcaaaa attcgcctcg attatccatt ctttttctcc tgcgcctgat gtttcttaaa
     1021 ccaactacgg aaactctctc cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct
     1081 aatcgcgcca aatttgagtg gtgttttgcc gccattgata aaccagcttg ccgcatgagc
     1141 gccggccatc atcccgactt tccacaatcc tagatgacta ttggcataag cgaacatttt
     1201 tatcgcccgt tgctctgctt ttgcggtgat ccctttttca gccatcaccc gacgatgacg
     1261 caaaatcagt tttgacagcg gaatacgcac cggacacacg ctgtcacaag cagtgcataa
     1321 agagcaggcg tagggtaaat ctttaaaatc tttatagccg ccaagtagcg gagaaatcac
     1381 cgcaccaatt ggccctggat aaatagagcc atatccatga ccgccaatat ggcgatatgc
     1441 cggacaagta ttcatacaag ccccgcagcg aatacagcgc agcacatccc gaaattcaga
     1501 ggccagcacc tcagaacgcc cgttatcgac aataaccaga tgaaactctt caggaccatc
     1561 aacgtgcccc gcttcgcgcg gtcctgtcag ccaggtgttg tatcccgtca aacgtgcacc
     1621 aacggcactg cgcgccagca tggtgatcaa tacatctacc tcggcaaacg tgggggcaat
     1681 acgctccatt cccatcactg caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt
     1741 accttcattg gtcaccaggc ataccgaacc tgtctctgcc accgcgaaat tacagccggt
     1801 aatacctatt tcagcactga ggaaatcttc gcggattttt tgccggatga ataaggtcat
     1861 cgcttcaggc gtttccggcc cctcatagcc cagacgttcg tgtagcactc gacggatctg
     1921 atggcgatct ttatgaattg ccgggaccac aacatgagat ggcggatctt gatccagctg
     1981 gagaatatat tcacccagat cggtttcaac cacctgaatg ccagcatcct gcaacacatg
     2041 attgacacca atctcttcgg tcaccatcga tttagatttc accaccttcc gggcattttt
     2101 gcgttgggca acctgtaaaa tgtagcgggt agcgtcttct ttgttttttg caaaccagac
     2161 gtgaccgccg ttttgcgtca ctttttctga gagctggtac agataagcgt cgagattact
     2221 cagaacatga tcacgtatct gggcggcccg atcgcgccac tcctcccagt gccccaattc
     2281 atcgaccatt ttttgccgat ttgccccaat acgctgctgc gcgtttgcca ccgctttgcg
     2341 catgatcgga tcttcaattt gctgacggat gcgtgtctta aaatctgtat tactggtttt
     2401 gatcgacatc tttatatcct cagcggctca tcaacacttc agcaatatgc atcactttga
     2461 ctttctgccc ttcccgttgt aatcgcccac tgatgttcag caggcaactc acgtcagcac
     2521 caattaaata ctcagggcgg acttccatca ggtgcccaac cttttctttc accatctcgc
     2581 cggatatttc ggccattttg accgagaacg tgccgccaaa tccgcagcag gtatcctgtt
     2641 cagcaaaggt caacagctcc agtccacgca catttttcag cagcgtaagt ggctcgtcct
     2701 tcactcccag cttacgggcc aggctacaag atgggtgata cacagctctc ccttgcaaac
     2761 tggcacctac atcgactacc cctaatttat taacaataaa agaggtgaga tcctgcatac
     2821 gcgcggcaac cttttcggca cgtgatgccc attcaggttc atccgccaga tacgtcgggt
     2881 aactttttac ggcataggtg caagagccag ccggtgaaat aatgggatcg tcgttatcct
     2941 ccagtgcggc gatcagattt ttcatccctg gaatcgcttc tttgatataa ccgctattga
     3001 tcgcaggctg accgcagcat ccctgtttct ccgggaaatt tacgcgacag ccgagttttt
     3061 ccagtagcag cacggagtct cgtgccattc ttgatttcag ggcgtcacca atacaggtga
     3121 caaagaaatt gacattcaca actaatactc cattacttca tgcccattta tgcccgatgt
     3181 ttccccccca ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc
     3241 aacatactac ccctcatttg tatgacaaat gataatcttt cacccaaacg tcaaatgcaa
     3301 taactaattt tatttctttc ggcatcgctc acattttatg gagaaatcga cgatcatttt
     3361 tggcataaga aatccattta aaaatggcta catcccttat acctattcag agctacttat
     3421 tcagggatta ttgagatata cactgatggt gtacaaacaa aatcaccgac aataatcacg
     3481 tatcaagtaa tagtcgggat tttatttaat tgaagcaatt tagtttgtat cttttattta
     3541 attactaagc cagaccactt caaaaaaaca tataaaaaag
//
LOCUS       DAJTMK010000275         3543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTMK010000275 DAJTMK010000000
VERSION     DAJTMK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.282"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..2744
                     /locus_tag="OO135_004043"
                     /pseudo
     CDS             1..2744
                     /locus_tag="OO135_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            2741..3245
                     /locus_tag="OO135_004044"
                     /pseudo
     CDS             2741..3245
                     /locus_tag="OO135_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3310..>3543
                     /locus_tag="OO135_004045"
     CDS             3310..>3543
                     /locus_tag="OO135_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5399656.1"
                     /translation="MTLAYRSLRIIHLYHCDYRGLPLTLISPDGATEWCAEYDEWGNL
                     LNEENPQHLQQLIRLPGQQYDEESGLYYNRHRYY"
BASE COUNT          943 a          892 c          976 g          732 t
ORIGIN      
        1 aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca gtttgacgcc
       61 gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata cagcccggat
      121 gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc ggcgttttac
      181 tatacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatacgc cgggaatatg
      241 atgaatgggg ccgtctgatt caggaaactg cccctgacgg cgatatcatc cgctaccgtt
      301 atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggt agccggaaaa
      361 ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc ggttatgtaa
      421 cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag gaagggctga
      481 gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac acgcagggcc
      541 atgaaacgcg gtatgaatac aacgccgccg gtgacctgac cgccgtcatt gccccggacg
      601 gcagcagaaa cgggacacag tacgatgcgt ggggaaaggc catccgtacc acgcagggcg
      661 agctgacgcg cagtatggaa tacgatgctg caggacgggt catccgcctg accagtgaaa
      721 acggcagcca caccaccttc cgttacgatg tactcgaccg gctgatacag gaaaccggct
      781 ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc cgcagcgagg
      841 atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg caccgcaccg
      901 tgaatggcga aaccgcagag cagtggcagt atgacaaacg tggctggctg acagacatca
      961 gccatctcag cgaagggcac cgggtgacgg tgcattacag gtatgatgag aaaggccggc
     1021 tgaccggtga gcgccagacg gtgcatcacc cgcagccgga agcactgctc tggcagcatg
     1081 agaccagaca cgcttacaac gcgcagggac tggcgaaccg ctgtataccg gacagcctgc
     1141 ccgccgtgga atggctggcc tacggcagcg gttacctggc aggcatgaag ctcggcgaca
     1201 caccgctggt ggatttcacc cgcgaccgcc tgcaccggga aacgctgcgc cgcttcggcc
     1261 gctatgaact caccaccgct tatacccctg ccgggcagtt acagagccag cacctgaaca
     1321 gcctgcagta tgaccgcgat tacacctgga acgacaacgg cgaactcatc cgcatcagca
     1381 gcccgcgcca gacccggagt tacagctaca gcaccaccgg caggctgacg gagaaaaccg
     1441 acctcatccc ggaagggggt atccgtacgg atgatgagcg cacccaccgg taccattacg
     1501 acagtcagca ccggctggtg cactacacgc ggacacaata tgcagagccg ctggtcgaaa
     1561 gtcgctatct ttacgacccg ctgggccgca gggtggcaaa acgggtgtgg cggcgtgaac
     1621 gtgacctgac gggctggatg tcgctgtcac ggaaaccgga agtgacctgg tacggctggg
     1681 acggcgaccg gctgaccaca atacagaacg acaggagccg catccagacg atttatcagc
     1741 cggggagctt cacgccactc atcagagttg aaaccgccac cggtgagctg gcgaaaacgc
     1801 agcgccgcag cctggcggat acccttcagc agtccggcgg cgaagacggt ggcagtgtgg
     1861 tgttcccgcc ggtgctggtg cagatgctcg accggctgga aagtgaaatc ctggctgacc
     1921 gggtgagtga ggaaagccgc cgctggctgg catcgtgcgg cctgacggtg gcgcagatgc
     1981 aaagccagat ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc
     2041 atcgcggcct gccgctggcg ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat
     2101 atgatgaatg gggcaacctg ctgagtgatg agaacccgca tcatctgcag cagctcattc
     2161 gcctgccggg tcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt
     2221 atgacccgct gcaggggcga tatatcactc aggacccaat tgggctgaag gggggatgga
     2281 atttttatca gtatccgttg aatccgatct cagagataga cccacaaggg ttaaatccgt
     2341 taattcttgg tgttgtagca ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc
     2401 aaagtgggca acaacgcaga gatcaatatg gacgtaaagt taatccagaa gccgacaaat
     2461 taacagatga acataagtca ggtgtaaatc ctggaggtaa ctgtagtccc cagaacctga
     2521 gtgacttaca aaatgaaaaa aacagattat gtaaccagtc tagagcttgc gctcctcaga
     2581 tgctgagaaa agaaatattg agacggtacg agataaattt ggcgtgcgct tcggtcagaa
     2641 agcagattaa taatcagtgc tttggtggtg gagataaggc ccatatggaa gaagagaata
     2701 aagcatataa aacagcagct gattgcagtg gcttaataaa atgaaaaaaa taattttctc
     2761 taacgagagt gcagtctatg atgaacttat gattcatttc ccatgccagc cattacctca
     2821 tatttcaaat gatattatag ggctggagga actggatatt gtatataatt tttttccaaa
     2881 agaaacaatg gaatgaaata atcaataatt taaaaataaa ggatgactct tatgctttag
     2941 agttaggaat tatattcctc cctgagaaag tattctgcta ttacatccct ctgtatattt
     3001 atgtctcgtt gtttaataag aatgattttt gggtttttga atccgatttt attcaacaat
     3061 atttatgtcc tgagtatcgg gattacgatg attttttgat ttttgttttt aatttttcag
     3121 atatacaact atctattatt gcacagttta tgtcttatga atcagacgct ggattctttt
     3181 atgcaagtaa agcttgtatg gatttttggg aagattattc acctctatta cataagaaaa
     3241 tttaatcgta aaaggtataa atggtttaat aaaaaacttg tctactcgtt ggagtagatg
     3301 aaaaataaga tgacgttggc atatagatca ctgcgaataa tccacctcta ccactgcgac
     3361 tatcgcggcc tgccgctgac gcttatcagc cctgacgggg caacagaatg gtgcgcagaa
     3421 tatgatgaat ggggcaacct gctgaatgaa gagaacccgc agcacctgca gcagcttatc
     3481 cgtctgccgg ggcagcagta tgatgaggag tccggcctgt attacaaccg ccaccgctat
     3541 tat
//
LOCUS       DAJTMK010000276         3527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTMK010000276 DAJTMK010000000
VERSION     DAJTMK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.283"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..494
                     /locus_tag="OO135_004046"
     CDS             <1..494
                     /locus_tag="OO135_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399657.1"
                     /translation="IAADKAKLLLEKGGEIAVRFKIPENVNLETTRNKYAFGLYWRIA
                     EWPGEGGEGHLSSFFVQSDKASINVAYHHTVNQQKELGTFGAFDHDWHTLAFKFKGSN
                     SINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLIDTVEVKANKAG
                     AAA"
     gene            634..814
                     /locus_tag="OO135_004047"
                     /pseudo
     CDS             634..814
                     /locus_tag="OO135_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
     gene            738..1085
                     /locus_tag="OO135_004048"
     CDS             738..1085
                     /locus_tag="OO135_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5399658.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRLNLESRLDAEVDAILDELLGVAVVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1162..1377
                     /locus_tag="OO135_004049"
     CDS             1162..1377
                     /locus_tag="OO135_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5399659.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAAWGE"
     gene            1382..1726
                     /locus_tag="OO135_004050"
     CDS             1382..1726
                     /locus_tag="OO135_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5399660.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1777..2310
                     /locus_tag="OO135_004051"
     CDS             1777..2310
                     /locus_tag="OO135_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5399661.1"
                     /translation="MNTKIKYGLSAAILALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2467..2649
                     /locus_tag="OO135_004052"
     CDS             2467..2649
                     /locus_tag="OO135_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399662.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2798..3265
                     /locus_tag="OO135_004053"
     CDS             2798..3265
                     /locus_tag="OO135_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5399663.1"
                     /translation="MNRMLSAFTVILLVVCGALWLATDHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGMDNATGPRLADTAERDYFTLRERLMTMQKQLEGAQEYIRTQCIN"
     gene            3289..3513
                     /locus_tag="OO135_004054"
     CDS             3289..3513
                     /locus_tag="OO135_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399664.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          931 a          759 c          988 g          849 t
ORIGIN      
        1 atattgctgc agacaaggca aaacttttac tggaaaaagg cggggaaatt gctgtccggt
       61 ttaaaatccc cgaaaacgtc aatcttgaga caaccagaaa caagtatgcc tttggtttgt
      121 actggcgaat agcggaatgg ccgggtgagg gtggtgaagg ccatctgagt tctttctttg
      181 tccagtcaga taaagccagt attaatgttg cataccatca tacagttaat caacaaaaag
      241 aacttggcac gtttggcgca ttcgaccatg actggcatac gcttgcattt aaatttaagg
      301 gcagtaacag cattaatgtt actccggtgc ttgatggtgt ggatggacag gcgtttgacc
      361 tggtgaaatg ggccaatact gctaatggac tcaacaggtt tgtcattacg gatattacag
      421 gtagtgcaga aacctaccct gtacttattg atacggtgga agttaaagca aacaaagctg
      481 gagcagccgc ataattgcta aaaaaagccg ccagcggcag gaatggaagc tggcggaggt
      541 aatcccaatg gagaatgtaa agaaaagatg ctttcgtaca ttggtttttt aaatgaaaac
      601 aattctcatt gtcaaccata acggtaagaa attatgacat ttattcatca ggtgatgctg
      661 tacttctgta cggcggtctg tgctgtatct tctttcgggt gggtacaggg ctgtgcgcga
      721 tttctggtac aggcagattg ataaaagggc cgctgagaaa atcagcgcca gtcagtcagc
      781 cggaagcaaa cccgaagaac ccgttactcc ttaacaaccc ctttcagcga gaaaatccca
      841 tgtcagaaat cacatccctg gtcactgcag aggcagtgaa ggaagtcctg cgctctgaag
      901 aagtccggag cgcactgaaa cagaaacttc gcctgaatct tgagtcgcgt cttgatgcag
      961 aagtggatgc cattctggat gagctgctgg gcgtagcagt ggttcctgag ccggaaggta
     1021 tcgcgggtga cgggagtgct tcagatggcg gtgaacctac acctgacagc gacatgatga
     1081 tgtaagcatg cgcaagggac catcggtgtg tgccggtggt ctttatattg ttgtgagctt
     1141 ccggattgcg ggagacgggg tatgtaccag atggaaaaaa tcacaacagg tgtgtcatac
     1201 accacgtcag cggtgggaac gggctactgg ttcctgcagt tgctggacag ggtttccccg
     1261 tctcagtggg cggcaatagg cgtgctgggg agtctgttgt ttgggctgct gacatatctg
     1321 actaacctgt atttcaaaat cagagaggac cgtcgtaagg cggcgtgggg agagtaaagc
     1381 gatgaagaaa aaatacgaac tggttgttaa agggataaat aattacccga ataagattac
     1441 tgttactgtg gcactggaaa ttggtgggta tccgtcactg ttgttgccag atgtggcgat
     1501 tagtcttgac cgtactgaag atgccacgct ggagttttac gaagctgagg cgaaaaagca
     1561 ggcgaagcag tttttcatgg atgttgctgc cgggttatgt gaaggggatg gtccgttgcc
     1621 ggaaaagcgc cccgtaattt tagaggcgca ggatgtgttg ataacctaca gaggaaaact
     1681 accgggaata attactggtt ctctgaagac tccaccgctg gcctgaagac ttaacatatc
     1741 cagggatttg aaatcgataa accctgataa atatccatga acaccaaaat caaatacggc
     1801 ctgtcggctg ccattctggc gctgattgcc gcaggtgcgc ctgcgcctga aatcctcgac
     1861 cagtttctgg atgaaaagga aggtaaccac accacggcat accgtgatgg cgcgggtatc
     1921 tggaccatct gccgcggtgc catcctggtg gatagtaaac ctgtcgtccc gggcatgaag
     1981 ttgtcgaagg aaaaatgcga ccaggtcaac gccattgagc gtgataaggc gctggcatgg
     2041 gtggagcgca atattaaagt gccgctgacc gaaccccaga aagcgggtat cgcgtcattt
     2101 tgtccctata acattggccc cggtaagtgt ttcccgtcga cgttttacag acggattaat
     2161 gctggtgacc gcaggggagc atgcgaagcg attcgctggt ggattaagga cggtggcaga
     2221 gactgccgta tccgctcaaa taattgctac ggtcaggtat cccggcgaga ccaggaaagt
     2281 gcgctggcgt gctgggacat cgacagataa gcagaatatt ttgctgaaaa ataaggcatg
     2341 agcacgcgga cggataacac gaaatcctgc gaactggcga aacgtaagtg aataaaagta
     2401 aaaaccccgt ttgttggcac caagcggggt tttgtgtttc tgaccttgag taaggcaagg
     2461 gagaacatgg cgaagtataa acgaattctg ttgaggttga ccatgaaaaa cggccttgaa
     2521 ctgaaagcgc ctgtaactga tgacatcagc agagcactgg cttttgccat taagtgggtg
     2581 gcggtcggtg ttgctgtgtc cccgatgctg tatgggctgg caaaactggt cattgcgttg
     2641 aaatcgtgaa gggaggatta agcatgtcag acaaactcat aacgctggcg aagatcctct
     2701 gtgtaattgt cggcatttca ttttcactaa tgctggttgc tctttttctt tccatggcct
     2761 ggatgatgtt gtcttcgtcg gggctgctgg ggtgaatata aaccgaatgc tttccgcgtt
     2821 taccgttatt ctgctggtgg tctgtggtgc gctgtggctg gcaacagacc attaccgtga
     2881 taacgccatc acctacaaag cgcagcgcga taaaaaagcc agagagctgg agctggcaaa
     2941 cgcaaccatt actgacatgc agcagcgcca gcgtgatgtt gctgcgcttg atgccagata
     3001 ctcaagggaa ttagccgatg cgagagctga aaatgaaact ctgcgtgctg atgttgccgc
     3061 tggtcgtaag cgcctgcgga tcaacgccac ctgctccggt accgtgcgtg aagccaccgg
     3121 cacctccgga atggataatg caaccggccc ccgactggca gacaccgctg aacgggatta
     3181 tttcaccctc agagagcggt tgatgacaat gcagaagcaa ctggaagggg cgcaggaata
     3241 tatccgcact cagtgtatta actagtattt ttgttatccg gagaatgcat gaagaaatta
     3301 ctggtaaccg taaagccttt tcagggaaca attccgttcc gtattttgca gcgtggtcgt
     3361 gttcttgttg aaggttcgtt cagtggtaaa tgtacgcaat tacactcccg gacctttcag
     3421 gtgaatgcca cgaatgaaga gctaaccgtt gagtgtacga tgaatgccgc taaatgccgc
     3481 atggtatccg ctgcattaca gccagtgtgt tgagcgacct tattaac
//
LOCUS       DAJTMK010000277         3431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTMK010000277 DAJTMK010000000
VERSION     DAJTMK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.284"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            139..1200
                     /locus_tag="OO135_004055"
     CDS             139..1200
                     /locus_tag="OO135_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR5399665.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARLRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKMFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(1313..2812)
                     /gene="ansP"
                     /locus_tag="OO135_004056"
     CDS             complement(1313..2812)
                     /gene="ansP"
                     /locus_tag="OO135_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR5399666.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            3079..>3431
                     /locus_tag="OO135_004057"
     CDS             3079..>3431
                     /locus_tag="OO135_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5399667.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWA"
BASE COUNT          953 a          881 c          829 g          768 t
ORIGIN      
        1 aaatcagtaa gttggcagca ttacccattt gagaaaataa tgattaccat tcccatttat
       61 aacaagagcg tgacgatgat tacgcttagc gaagcattgt gaagcagcaa aaatattggt
      121 tcatcaaagg gagtcgtcat gcatttacgt catctgtttt catcgcgcct gcgtggttca
      181 ttactgttag gttcattgct tgttgcttca tcattcagta cgcaggccgc agaagaaatg
      241 ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt atagccagca agaaaacgcg
      301 ctgtggctcg ccacttcgca aagccgcaaa ctggataaag gtggcgtggt ttatcgtctt
      361 gatccggtca ctctggaagt gacgcaggcg atccataacg atctcaagcc gtttggtgcc
      421 accatcaata acacgactca gacgttgtgg tttggtaaca ccgtaaacag cgcggtcacg
      481 gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg tgctggatga tcgtaagcgc
      541 acggaagagg tgcgcccgct gcaaccgcgt gagctggtag ctgatgatgc cacgaacacc
      601 gtttacatca gtggtattgg taaagagagc gtgatttggg tcgttgatgg cgagaatatc
      661 aaactgaaaa ccgccatcca gaacaccggt aaaatgagta ccggtctggc gcttgatagc
      721 aaaggcaaac gtctttacac cactaacgct gacggcgaat tgattaccat cgacaccgcc
      781 gacaataaaa tcctcagccg taaaaagctg ctggatgacg gcaaagagca cttctttatc
      841 aacatcagcc ttgataccgc caggctgcgt gcatttatca ccgattctaa agcggcagaa
      901 gtgttagtgg tcgatacccg taatggcaat attctggcga aggttgcggc accggaatca
      961 ctggctgtgc tgtttaaccc cgcgcgtaat gaagcctacg taacgcatcg tcaggcaggt
     1021 aaagtcagtg tgattgacgc gaaaagctat aaagtggtga aaatgttcga tacgccgact
     1081 catccaaaca gcctggcgct gtctgccgat ggcaaaacgc tgtatgtcag tgtgaaacaa
     1141 aaatccacta aacagcagga agctacccag ccagacgatg tgattcgtat tgcgctgtaa
     1201 tagatatggc gagggtgaaa acggtgagac ttgcctgcct tttatcacgg cgtatccggc
     1261 actttcgtca gtaatctgaa tttgcccggc acgtagccgg gcaattttgc aattaactcg
     1321 ccgtttcagg cttaaacaca atttcctgtt tttcttcatc ttcctcgacg actggcgcag
     1381 tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca gcctataacc agcagaatac
     1441 cgataatcgg cagcgccgcg atagtataag tcccgttcgg gtaatcgaac gccatcagca
     1501 caaggacact cagtaaaaac agtaatgtca gccaggaagt gaagggcgcg ccaggcagtt
     1561 taaaactgac atccgctgct ttgccttctt taatcgcttt acgcaggcgc atctggcaca
     1621 cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc gaagttcaac acaatctcaa
     1681 atacgcgcga cggcaccaga tagttgagga atacgccgac gacatacaca accagtgtcg
     1741 ctagaatccc ggcatacggc acatgctgac gactcatttt cgccataaaa ctcggtgcgg
     1801 aaccgcccat cgccattgag cgcagaatac gtccggtgca gtacagacct gagttcaggc
     1861 tggagagggc agcggtcagc accacaatgt tcataatgct gccgatatat ggcacaccca
     1921 gtttagagaa aaacgtcacg aacggacttt gccccgcctg atacgcgctc cacggcaata
     1981 acataaccag caacaccacg gagccgacgt aaaacaggcc aatacgccaa atcacactgt
     2041 taatggcttt aggcaccatg gtctgcggat ctttacattc tccggcagct gtacccacca
     2101 tttcaatgga ggcaaaagca aacactacgc cctgaatcaa caccagcgca ggcagcaaac
     2161 cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa gccagtggtg ttgccatcca
     2221 gcggctgacc actaccgagg aacactgtac ccacgaccaa aaaggtcaca atggcgagca
     2281 ctttaataag cgcaaaccag aactccatct ccgcaaacca tttcacaccg atcatattca
     2341 tggtgccaac gatggtaagt gcagcgagcg caaagaccca ctgcggcacg ccgccaaacg
     2401 caccccagta atgcatatac agagcgacgg cggtgatatc aacaatcccc gtcatcgccc
     2461 agttgatgaa gtacatccag ccagcaacat aagcggcttt ctcacccaaa aactcacggg
     2521 cataagagac aaaactgcca ctggaagggc ggtgtagcac cagctcaccc aatgcacgca
     2581 gaataaaaaa cgaaaacaag ccacaaatta aataaaccag tgccagtgct ggacccgcca
     2641 tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc aatcgcgccg ccaatggcga
     2701 tcatctgtac ctggcgattg cccatcgctt tgtgataccc ctcttcgtgg gcattaagcc
     2761 agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc gtgtttactc attgctctcc
     2821 ctgattgctt taatgaaaaa gtcatataag ttgccatgaa caatatttat tcatcctggc
     2881 gatgatagtt atcaccatta ttctttatgg gtataaaact atcggcgcag catcctaccc
     2941 gttatcgata tacgatgcaa aacatcccct tacaatcctg aaggggattc atacaactga
     3001 cgaaaaaatg acaaatactt ttgctggtta acctgtgtac tgtcctacac ttaatcttta
     3061 aaagattgtg aggggcatat gattaaggtg tatggcgtac ccggatgggg ctctacaatc
     3121 agtgagctga tgctaacact ggctgatatt ccttatcagt tcgttgatgt cagtggtttt
     3181 gaccacgagg gaacttcacg cgagttattg aaaaccctga acccgttgtg tcagattccg
     3241 acactggcgc tggaaaatga cgagattatg acggagacag cggcgattgc attgatggtt
     3301 ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg ccgaacgtca gcagtttcaa
     3361 cggctattgg tctggctggt tgctaatgtc tatccaacat tcactttcgc cgattaccct
     3421 gagcgttggg c
//
LOCUS       DAJTMK010000278         3392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTMK010000278 DAJTMK010000000
VERSION     DAJTMK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.285"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..735
                     /locus_tag="OO135_004058"
     CDS             <1..735
                     /locus_tag="OO135_004058"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5399668.1"
                     /translation="AEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTIVYNV
                     NDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVDGPRG
                     KDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVSILGK
                     KVTAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGDLQLV
                     KTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(777..2216)
                     /gene="aldA"
                     /locus_tag="OO135_004059"
     CDS             complement(777..2216)
                     /gene="aldA"
                     /locus_tag="OO135_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5399669.1"
                     /translation="MSVPVQHPMYIDGQFVTWLGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2413..3213)
                     /locus_tag="OO135_004060"
     CDS             complement(2413..3213)
                     /locus_tag="OO135_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5399670.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
BASE COUNT          868 a          878 c          804 g          842 t
ORIGIN      
        1 gcagaaatca ttgtcgaatg tactggcttt tatacctccg ccgagaaatc gcaggcgcat
       61 cttgatgctg gtgcgaagaa ggtgttgatt tccgcccctg ccggtgaaat gaaaactatc
      121 gtttataacg tcaatgacga cactctggat ggcaacgaca ccattgtttc cgtggcgtca
      181 tgcaccacta actgtcttgc gccgatggcc aaagccttgc atgacagttt cgggatagaa
      241 gtcggcacga tgacgaccat tcatgcctat actggcaccc agtcactggt ggatggccca
      301 cgtggtaaag atttacgtgc ttcacgcgca gcggcagaaa atatcattcc ccacactacg
      361 ggggcggcaa aagccattgg tctggagatc ccggaactga gcggcaaact gaaaggtcat
      421 gcgcaacgcg tgccggtgaa aacaggttcg gtcactgaac tggtatcgat tctcggaaaa
      481 aaagtgactg ccgaagaggt gaataacgca cttaaacaag caaccaccaa taacgagtca
      541 tttggttata ccgatgaaga aatagtctct tccgatatca ttggcagcca tttcggttcg
      601 gtgtttgatg ccacgcaaac ggaaattacc gccgtgggcg atttacaact ggtgaaaacg
      661 gtcgcctggt acgataacga atatggcttc gtcacgcagc ttattcgcac cctcgaaaaa
      721 ttcgctaaac tctgacgcgc acaggcggag gaaaaaacct ccgcctcttt tactcattac
      781 gactgtaaat aaaccacctg ggtctgcaga tattcatgca ggccatgttt accatctgcg
      841 ccgccaatac cggatttacg ccatccggcg tggaagcctt gcatagcttc gaagttttca
      901 cggttgatgt aagtttcacc aaacttcagc cctttaatgg ctttcatcgc gacgttcaga
      961 ttttgggtat agattgatga ggtcaggccg taatcactgt cattagccat tgagatagcc
     1021 tcttccagcg tgtcaaatgc gaccaccggc agaaccgggc caaaggtttc ctcatgcata
     1081 atcgacattt cctggcgaac atccagcagc aatgtcggcg gataataata tcctttcccc
     1141 tctaccgctt tgccacccaa cgccactctc gccccttctt ctactgcgcg cgccactttt
     1201 tgctcaaccc tttccaacgc cgcggcgtta atcaacggcc ccatcgcaat gtcgttgcgt
     1261 tcagcggggt taccaaattg aaccgcctgc atcgcttcac ccagccgatt gacgaactga
     1321 tcataaatgc ctttctgtac ataaacacgt tctgcacagt tacacacttg cccactatta
     1381 atgacgcgtg aatcaacgat ggctttgact gccagttcaa gatcggcatc gtccattacg
     1441 atagctggtg ctttaccccc caattccaga cacactttgg tgatgttttt cgccgcagtc
     1501 gccatgatct tctcacctgc agagacgctg cctgtcatac tgaccattgc gacctttggg
     1561 ttacccgcca gttcttgccc aacggtttca ccacgcccca gtacaaggtt aaacacgccg
     1621 cgcggaaggc ctatttcatc gacgattttg gcgaatgcaa tcgcattgtt tggcgtaaat
     1681 tcactaggtt taatgacgat ggtattaccg gtcaaaagag cgggagccat tttgcgggca
     1741 atgaggaaga acgggaagtt ccacggcaga atgccggtag tcacgccaag cgcacgttta
     1801 aacaaaagaa tattttctcc tggacgatcg ctttgaataa tctcgccctc gtaacgccgt
     1861 gcccactccg ccatgtaatc gatatagtcg gcagtaaaag ccacttcgac ttcagccagc
     1921 tgctggatct tgcccccttc ttcaacaatc agcgcactga tttcactggc gcgttcgcgg
     1981 atcccggcgg agattttgcg caaccaactg gcgcgttcaa tagcaggcaa cgcttcccat
     2041 tctggttgtg cacgttctgc tgcatcgatt gccttacggg catcctcggc ctgaccatcg
     2101 ggtatgcggg aaatgacagc ctctgtagca gggtttacca catcaatcca tgcgtctcca
     2161 agccaggtaa caaactgtcc atcgatatac ataggatgtt gaacgggtac tgacatgggc
     2221 gactcctgtg atttatatgt ttgttcttgg taaatacatt gttaattaac atcaatcatc
     2281 atagagacgg catcgtgaaa ggacaattct gtgaagggct tcataaaaac aagtgtaaaa
     2341 ggaataaaag gcagaaataa aagtcaggag tcatgcccgt ttgcaaacaa gtggttcaca
     2401 aacgggcaat ttttaacgta acgagcgact ttccatcgcc tcgatgagca ccgcatcgtg
     2461 tttaagcgtt tgccatgcat ggatgacttc tgccggaaaa tcaacgtgaa cgataaaatc
     2521 tcgcccgcgg ggaccgtagc catcgctatc atcgcgtaaa cgcggcagct cgccagtgag
     2581 tagtgagaga taacgctcaa ccggccataa tccttgtaac tgattaataa agattacact
     2641 gtctgcgtta tttcctaact gaggaacgaa tccgggataa cttaaccagc gtggtgcatc
     2701 gggattgtcc ccagtcatac ggcggaacgt cgccatcgtg cgccgctgca tggtggggtc
     2761 ctgaacaacg atagccgtat gaactcgttc tacggcctga ttaaatagcg cgatgctaaa
     2821 gcgtgcgttt tcaccgcagt ttgttgactg gtcttcaatc cagatttttt catgcggaat
     2881 gtgccagaac tgatgagcga tatccgccag gatggtcgct tctgctctgc cagtggtgcg
     2941 gatagtgttg tagtgcggat gctgtgcgat ggcgctatac aaaaaagttg tcgagtgacc
     3001 gataccacca ctaatcagta aaggaatttg ctgatcgcga gcaatcttac atgccgcgtc
     3061 gatagtcggc ataaccgcat tgcctgcaag gatcacgcaa tcggcctgat acggcacctc
     3121 accggagaaa tcatcctgcg ccagccactg tccgataaca tttatggcat ctatagttgc
     3181 cggcgaaagc gtcggaaacg gggtaatgtt catggttcct ccttatcgtt cccttaagaa
     3241 tactgcgtga gataatagtg agaagctgag cactctgaaa ggaggaagtc gtatgtcctt
     3301 aaccgcactc cgcaagcctg acatatcttt taaggaaatg ctgtagataa acattattca
     3361 tcttgcagca gagaattgta tttttctgaa tt
//
LOCUS       DAJTMK010000279         3192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTMK010000279 DAJTMK010000000
VERSION     DAJTMK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.286"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            102..3004
                     /locus_tag="OO135_004061"
     rRNA            102..3004
                     /locus_tag="OO135_004061"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3100..>3192
                     /gene="rrf"
                     /locus_tag="OO135_004062"
     rRNA            3100..>3192
                     /gene="rrf"
                     /locus_tag="OO135_004062"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          698 c          984 g          659 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgaatcgta agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatattg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cactatgtgt tgttgggtag gggagcgttc tgtaagcctg tgaaggtgta
     1321 ctgtgaggta tgctggaggt atcagaagtg cgaatgctga cataagtaac gataaagcgg
     1381 gtgaaaagcc cgctcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt
     1441 gagtcgactc ctaaggcgag gccgaaaggc gtagtcgatg ggaaacaggt taatattcct
     1501 gtacttggtg ttactgcgaa ggggggacgg agaaggctat gttggccggg cgacggttgt
     1561 cccggtttaa gcgtgtaggc tggttttcca ggcaaatccg gaaaactaag gctgaggcgt
     1621 gatgacgagg cactacggtg ctgaagcaac aaatgccctg cttccaggaa aagcctctaa
     1681 gcatcaggta acatcaaatc gtaccccaaa ccgacacagg tggtcaggta gagaatacca
     1741 aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga
     1801 aggcacgctg atatgtaggt gaagtccctc gcggatggag ctgaaatcag tcgaagatac
     1861 cagctggctg caactgttta ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat
     1921 acggtgtgac gcctgcccgg tgccggaagg ttaattgatg gggtcagcgc aagcgaagct
     1981 cttgatcgaa gccccggtaa acggcggccg taactataac ggtcctaagg tagcgaaatt
     2041 ccttgtcggg taagttccga cctgcacgaa tggcgtaatg atggccaggc tgtctccacc
     2101 cgagactcag tgaaattgaa ctcgctgtga agatgcagtg tacccgcggc aagacggaaa
     2161 gaccccgtga acctttacta tagcttgaca ctgaacattg agccttgatg tgtaggatag
     2221 gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc cgaccttgaa ataccaccct
     2281 ttaatgtttg atgttctaac gttgacccgt aatccgggtt gcggacagtg tctggtgggt
     2341 agtttgactg gggcggtctc ctcctaaaga gtaacggagg agcacgaagg ttggctaatc
     2401 ctggtcggac atcaggaggt tagtgcaatg gcataagcca gcttgactgc gagcgtgacg
     2461 gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg ttctgaatgg aagggccatc
     2521 gctcaacgga taaaaggtac tccggggata acaggctgat accgcccaag agttcatatc
     2581 gacggcggtg tttggcacct cgatgtcggc tcatcacatc ctggggctga agtaggtccc
     2641 aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct gggtttagaa cgtcgtgaga
     2701 cagttcggtc cctatctgcc gtgggcgctg gagaactgag gggggctgct cctagtacga
     2761 gaggaccgga gtggacgcat cactggtgtt cgggttgtca tgccaatggc actgcccggt
     2821 agctaaatgc ggaagagata agtgctgaaa gcatctaagc acgaaacttg ccccgagatg
     2881 agttctccct gactccttga gagtcctgaa ggaacgttga agacgacgac gttgataggc
     2941 cgggtgtgta agcgcagcga tgcgttgagc taaccggtac taatgaaccg tgaggcttaa
     3001 ccttacaacg ccgaaggtgt tttggcggat gagagaaaga ttttcagcct gatacagatt
     3061 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggccg tagcgcggtg
     3121 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg
     3181 gggtctcccc at
//
LOCUS       DAJTMK010000280         3181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTMK010000280 DAJTMK010000000
VERSION     DAJTMK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.287"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            353..512
                     /locus_tag="OO135_004063"
                     /pseudo
     CDS             353..512
                     /locus_tag="OO135_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(730..2820)
                     /gene="iha"
                     /locus_tag="OO135_004064"
     CDS             complement(730..2820)
                     /gene="iha"
                     /locus_tag="OO135_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR5399671.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDMADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          836 c          734 g          816 t
ORIGIN      
        1 gttgatctga cctctcagta aatatcaata cggttctggc gagccgctta cgtgcagcag
       61 tcttatccgg cagtttaaag gggaggacgg agaccaatga ggcactggag cagattcagt
      121 gcagaggtga cctgctttat caaacttaat tgtgacaaaa tggcgattga gaaggtgcaa
      181 ttgacttatc cggattcggt cgtggcgagg tggtgcccct gcattctgct aaccgggtta
      241 tgagctagga gtcagcgata gacaaaggga cttatctgac cgggcccccg gaaaaatggc
      301 gggagagaat gataccttcc tgctgacgag accggacttt gagatgacga gcgatagtgc
      361 ggcagtgatg tacagcctga tcggtagctg caaaacttaa cggaatcgag ccggaaacgt
      421 ggctacgcca cgtgatcagt gtaatcaaca cctggcctgc caaccgcatg aaagagttgt
      481 tgccctggaa cgtcactctc tctgtaaact aatttttacc ccacgtcctt cacggtgcgc
      541 ttacactctg gacaagtatg taaatgataa gttaagaata atccgtgcat ttcctgtgac
      601 gatattagtt gttggtaacg aaatctcatt agcggatcgg aaatgcactt aacctaatac
      661 gttgccactg ttccgccagg agttgcagtg gcaacgtatt ctaccgtcag tgatagcgtt
      721 ttgttattat cagaactgat agttcagcga catccagtaa tttcgctcag gtatcacata
      781 tcctgttgtt gatgatcccg tctggaagta atcaccggca tacagcgtac tcttaccggc
      841 actgtacagg ctcacgtcac tgtaatcctt gttgagcagg ttattcaccg cagcattcag
      901 cgtcagggca tccgtcatct tccacgacag acctgcatcc accaccgtcc aggctttcag
      961 gtattctcct ttctcatcat acactttctt ctgtacagcg ctcagtgacg aataattctg
     1021 ggtgaaacgt ggtgttttcc cgcgataacg ggcacccagc catgatgcca cctcttcggt
     1081 gatctgccag ttcagtttcg cattcaccat gtgttcaggg gtataactca gcggcgcacc
     1141 tttgttatca ccatcacgtt gttcacttcg ggtccaggtg taattcagtg acagcgtgac
     1201 atcctctgac cacagcggca atgtgccggc aaattccaca ccgtgcaacc gggcctttcc
     1261 gctgtttaca tagctattgg tgttatcatt tatggaataa gagacaatct tgttggagaa
     1321 gtcagtcata aaacctgtga cattggcatt cagaccggcg gggttatcgt aatacacccc
     1381 agcctcataa ctgacgctct cttccggctt caggtcgggg ttaccaagta gatttgtttt
     1441 tccctgcccg gacacaccac taatcccttt atgtagctgc cccattctgg gtgccttata
     1501 tcccgtggtc acaccgcctt tcagcgtcca ggcatctgcc atatcccaga ccagatatgc
     1561 acgcggactg aagtgtcccc cgaattgctc atgatgttca tagcggctgc ccgcagtcag
     1621 cgcaagtgca tccgtgagat gccactcatc ctcagcaaat accgaccagc ttttctgccg
     1681 gaaagtttca cctgtgctgg caaggacaac tccgtctttc atggacgagc tctgaaactc
     1741 gccccccacc gtaaccagat gagattctcc cagaggggta agcagtaatg aattcaggat
     1801 aaggttcgat tccttaagct cccgcggctg accggcaagc ccccatttgt cgcgcttcag
     1861 tacactgcgt acgagctcac gacctttatt ttctgtctcg ttccagttca gatacgattt
     1921 ccatgttccg aaggtgaaag tatgatcata gccagctgaa attttgttcc gctcatagcg
     1981 cagggtccgg tcatatcccc ccgtcagact ccccagttgc ccatcccggt tatcataacg
     2041 ctgccgggtg gtatccatat caaaccagag cacatcctgc tccgacgcct tccagtcaag
     2101 acgtgcacca agattataat tctgtgactc cgtgggataa ggaatacgcg tggctgctgt
     2161 atcgctcagt gatgtgaccg atgagccctg acgctgttgt gtgctaccgc gtacctgcag
     2221 gctgacagaa tcatccacaa gggggccact gctccagaaa ttaaactggc tgctgttacc
     2281 ccatttgttg ctttcctgca gatttagccc tgcattgacg gaagagagcc atttgtctgc
     2341 attctttctg gtaatgatat tcaccacacc gcccatcgca tcagagccat acagtgtgga
     2401 catcggcccc ctgataacct caatacgctc aatggcggcc agagggggca tgaacccggt
     2461 attcatggca gaaaaaccgt tgggagtcac gtcactgctt ccgccctgac gaacaccatc
     2521 aatcagtatc agcgtgtaac tggctggcat tcctcggatg ctgatttcca gcccaccggt
     2581 tttacccgta ccactttcaa catccacacc ctctactgat ctcagagcct ccgccagatc
     2641 gtggtactgg ctggactgca attcctcctg gctaatcaca gaaacactgg cggctgcgtt
     2701 agtcagcttt ttctcatagc cggatgccga gacaatcatc acatcctctg cagcagctat
     2761 gctgctggct gaaaatccga gacagggaat gactacggaa gccagagtgg ttattcgcat
     2821 attactaatc tccgatcatg ttaaccggca agacaataca caaatacaaa caataatgag
     2881 aataatttat atttgccttt tacaaaaaat aaagattatt aacaaaacta aatataaata
     2941 acaactgcct cggcatgcca gttaacagct atccgtatgg cgtgttcggt tttgcgaata
     3001 aaaaatggtt actgtagaag cggatttcca ccacatgtca accatgaatg gtcagtggtg
     3061 cgagacgact gggatgattt ggtcgatgac ttcttccggt gaatactgat agaaacattg
     3121 aaatattgta agagaaagcg ttcaaaattc tgtggcacgt gaaaaattct acagcgtcat
     3181 c
//
LOCUS       DAJTMK010000281         3165 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTMK010000281 DAJTMK010000000
VERSION     DAJTMK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.288"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..191)
                     /locus_tag="OO135_004065"
     CDS             complement(<1..191)
                     /locus_tag="OO135_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5399672.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSSEG"
     gene            complement(327..1430)
                     /locus_tag="OO135_004066"
     CDS             complement(327..1430)
                     /locus_tag="OO135_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399673.1"
                     /translation="MNSLARRLRDYDKTDMVMGRVYSNVFDCGFIDMEQSWIQLLLIF
                     SNKNDSVSKEIKEAIENNYITYREKQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKE
                     NLRKLLQEHINMLKKDLDMRLQDAAIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSI
                     DFKQASDCFTKHKISIKIDKEPYAEGIESIPYINEGDIQADLILKDIQRIILSNLFST
                     GCSQHTVIEDFNMLIDHIKSSADLAGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKND
                     DGSMNITTESGTRKVYFLPFVNQPFSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSD
                     SDKFKLTLNYELNIVFEGNADLKIAHSQRVTSE"
     gene            complement(1731..3165)
                     /locus_tag="OO135_004067"
                     /pseudo
     CDS             complement(1731..3165)
                     /locus_tag="OO135_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005113346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          974 a          526 c          562 g         1103 t
ORIGIN      
        1 atgccctctg atgagtgaat gggggaggcg tgctttgaag tgaatttgtg gaaggcttcc
       61 agtgttgaga aacatacgcc gcattctaga ttgttacact gataatactt ttgtctcacg
      121 gtgtttgaat cattttccgg acgactggtg cggatacggg cagatgcgcc acaaagcgga
      181 caacggaaca tagcgacctc tcttattgta gtgcttgcac tattctaggt tgtattctct
      241 aagatttaaa catgactttc tctatataat gatttttagc attagttaat aaacgaacgg
      301 cactctaagg agcgccgtca aagatattat tctgatgtca ctctttgcga gtgagctatt
      361 tttaaatcag cgtttccttc aaatactata ttcaactcat agttaagagt tagcttgaat
      421 ttatctgagt cacttttgat attctctgaa gtgacattta ctaacttatt attatcatat
      481 tctctaataa ttaatttagt gaagtagtta tcctttacca caagagaaaa tggttgatta
      541 acaaatggta agaagtaaac cttacgggta ccggattcag tagttatgtt catagagcca
      601 tcatcatttt ttctcattag ttctctgagg ttgggattat cgaaaagcat acgattatat
      661 tgctgaaaga tctctttact catcacaaga accagtttgc cagccaagtc agcagatgat
      721 tttatgtgat caatcaacat attgaaatct tctattacgg tatgttgcga acagccagtg
      781 ctaaataaat ttgaaagtat tattctctgg atgtccttga gaattaagtc tgcttgaata
      841 tcaccttcat tgatataagg gatggattct attccttcgg catacggttc tttatctatc
      901 ttaatagata tcttatgttt tgtgaaacaa tcgctagcct gcttaaaatc aatagatttg
      961 aaaagtgaaa gagggagcgt tttcttgatg cgtttacgaa gatgttctga tgttttacgt
     1021 gcagtgctat ctagtcgatg tacatctatg gcagcatcct gaagtctcat atcaagatct
     1081 ttctttagca tgttaatatg ctcttgaagc aactttctta agttttcttt ttttgtttgg
     1141 agatcgtcca atgttaaagt cagcttgatt ttagtgtatt cgatggagtt acaaattttt
     1201 gataatactc caatgagtcg ttgcttctca cgatatgtta tatagttgtt ttctattgct
     1261 tcttttattt cttttgatac gctatcgttc ttgttagaaa aaattaataa tagctgaatc
     1321 catgattgtt ccatatcaat aaatccacag tcaaaaacat tactgtaaac tctgcccatc
     1381 accatgtctg ttttatcata gtcacgtaaa cgccttgcta aactattcat ctacccattg
     1441 tgctccatat tttcagacca tgtccacagg cagattctaa ttaatatctt gattagttgg
     1501 cttgcattat caatttcttc agtgggagtt tcatatccac cggtttggtc aataagttta
     1561 ccctctaaaa taagtttaat gtaatgtgtc attagtttgt cagaagtatt gattcttgat
     1621 gtcagataaa gacatgtgca tatacgcatg tcagtcaacg catttatgat ggcaatattg
     1681 aaccactctc tgtctgaatt gaagtttagt tttttggata tgaaaaaaac cgggttaaaa
     1741 agctggcctt tgtaactgta tttttctacc gaatggctgt caatattcat tgagtgctgc
     1801 catcggttaa taagatcgac ggctgtatca atagtagaag gatcgcgagt ctgtgttgca
     1861 tcaataatca tcgatggaag ttcttgtagt tgcgttttgc aaaataattc aaataaatct
     1921 gaattctctg tattgcagaa acgaaagttt atgctttctt gatattgctc ccaaacagtg
     1981 gtataggttc ttaatatacg attataacgt gaacgaagcg aggcatttaa gagtgattgg
     2041 ttgtttcgga accattctgt taatattgac cattgtgaac gagtaattcc catatagata
     2101 caaagttcat tgctgataag atgttttcta ttattgataa tacgcactcc gctccaaaga
     2161 cataaatcaa aaaatctttc agaaagatta attttatcca tggaattatt tgatatttta
     2221 taaacttcat ctgaaaactc atattgaaaa ctccgttcaa ataattctgt agtgatcagt
     2281 aagaagttat tgttggtatt atcatcttga aaaaaataca aatcgcagag attattaaag
     2341 ttattaatta atgcagagat agcattgtca aaactaatga gatcagaatc ccgcagatac
     2401 atatacgtat ctgctgttat agcctgcatt gtaagactta cggaaaaatc tcctccttta
     2461 atcggagccc tataaaaaca ttgcttaagt atttttatta ataagctgtc tggtttgata
     2521 tttttaacgc taaaaagtat cttacccgtg gattctgttt cttttgtacg attatcacca
     2581 atgacaaatg acgcaaaatg attcttcttt tttagttggt aattaataag attaagagta
     2641 agttgaaaag gacggtgata taaacttaac tcatcttcct tagaataact gctagccaca
     2701 gatatgtatt ctcctttgta atcggcttgc ttgatttcta aatttgaata gttatgtttt
     2761 tgatagatag ggtataacct aagcttggct gagattttgt tacaaatatg gggcattact
     2821 atttcgaagg caatgtatcg ctttataatt atttgccgtt ttacatcatc aagaatttca
     2881 agactcacga taaagaacca aatagaaaga actatgttta tcaaaagcca taatatgctg
     2941 attagacaag ctataccata aagataactt ccaaagacag ttttaattag tacactgaga
     3001 agtataaatc ctgataagaa aagcccgctg agtccagcaa gcatgaatcc tgagtaacgc
     3061 tgataagcag tttgggctat ttttctatct gccttttttg ggaataagac acttactaaa
     3121 cctataacaa gggggtatac aataccaata attgtcaatt ggcca
//
LOCUS       DAJTMK010000282         3087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTMK010000282 DAJTMK010000000
VERSION     DAJTMK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.289"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..191)
                     /locus_tag="OO135_004068"
     CDS             complement(<1..191)
                     /locus_tag="OO135_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5399674.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQR"
     gene            384..886
                     /locus_tag="OO135_004069"
                     /pseudo
     CDS             384..886
                     /locus_tag="OO135_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(932..1348)
                     /gene="rraB"
                     /locus_tag="OO135_004070"
     CDS             complement(932..1348)
                     /gene="rraB"
                     /locus_tag="OO135_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5399675.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1510..2514
                     /gene="argF"
                     /locus_tag="OO135_004071"
     CDS             1510..2514
                     /gene="argF"
                     /locus_tag="OO135_004071"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5399676.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2586..2909)
                     /locus_tag="OO135_004072"
     CDS             complement(2586..2909)
                     /locus_tag="OO135_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399677.1"
                     /translation="MKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVNN
                     NPGYVVEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKK
                     LGWNN"
     gene            complement(2896..>3087)
                     /locus_tag="OO135_004073"
     CDS             complement(2896..>3087)
                     /locus_tag="OO135_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399678.1"
                     /translation="SRKLQELIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEV
                     PVSILVNDIFFEGFFHEAT"
BASE COUNT          865 a          702 c          657 g          863 t
ORIGIN      
        1 cgttgtccgt taacttccat attagcatag agataacgct tacatttcat taatgcccat
       61 ggtaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca aataagaaaa
      121 tatctctcac ctgtaccatg aaaaacaaac gaatgggacg gaacatccat ttcattaata
      181 acttgagcca taaaaattcc ctgaattaaa aaatccattg atataattta tatatacaga
      241 cagtaaaatg ttctgccacg ttacaatact cttatcagta acaggctggc aaaaacttta
      301 ttgctttata agatgcttgc gcacgtaaga agagagtggt attaaacaaa tgtataatta
      361 ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct gtaatgcgtc
      421 gcctgacgct acaggataat cctgctattg cccgcgtcat tcgtcaggta tccgccgaat
      481 acggtcttac cgctgataaa ggctacacca tcgccgatcc gaatcttgac gaactgtatc
      541 aagtgtatag tcagccgggt catgcatatt gggtggttga gtacgagggt gaagtggtcg
      601 gcggcggtgg gattgcgcca ttagcaggta gtgaatcgga catttgcgaa ctgcaaaaga
      661 tgtattttct tccggctatt cgcggtaaag ggctggcgaa aaaactggcc ttaatggcga
      721 tggagcaggc acgatagatg ggcttcaaac gctgctatct ggaaacgacc gctttttaaa
      781 ggaagccatt gcgctttatg agcatttggg ctttgagcat atcgactatg cacttggctg
      841 cacgggccat gttgattgtg aagtgcggat gttgcgcgaa ctctaaaaaa atgcccgcta
      901 gtgtgtgcta tcgggcattt taatcaatta attagtggcg aaccccgtca tcgtcttcat
      961 cgacaaaatc ttcatcgtcg ccgtcttcgc cattaggatc ttcaaaataa gtgccccaac
     1021 cgtcgtactc aacgtcaaat ttctccgcca atgtcatcag ttgttcaacc tgggcatcga
     1081 tcagatcggc attcaacgcg cactcgctga ggatgtcgca gcaaatcacg atatcaccat
     1141 cttcaacttc cagctcttct ggatcggtca cttcgtaacc gagtttaaac gcttcaactg
     1201 ctgctttttc cagggtttcc agatcgtctg cggaaagatg gtgttcgatg gtgtacagcg
     1261 cgtccggatc gctgccatct tccagtaatt cttcaataat caaacgagtt tcttcacgct
     1321 gttcttccag ttgttccggg tttgccatgg ctcattcctc ttaatgtcct gccgatactc
     1381 ttattgtcac ataccactga cattgcctcc acctttgtga caaagattta tgctttagac
     1441 ttgcagatga ataatcatcc atataaattg aattttaatt cattgaggcg ttagccacag
     1501 gagggatata tgtccgggtt ttatcataag catttcctga aattactcga tttcacgcca
     1561 gctgaactca acagcctgct gcagttagcc gcgaagctga aagccgataa gaaaagcggc
     1621 aaagaagaag ccaaactcac tggtaaaaac atcgcgctca tcttcgaaaa agactcgacc
     1681 cgtacccgat gctctttcga agttgccgca tatgaccagg gtgctcgcgt tacttatctc
     1741 ggcccaagcg gcagtcagat tggtcataaa gagtcgatta aagacactgc ccgcgtgctg
     1801 ggtcgcatgt atgacggtat tcagtatcgc ggctatggtc aggagattgc cgaaacactg
     1861 gcggaatacg ctggcgtgcc ggtatggaac ggcctgacca atgagtttca ccccacgcag
     1921 ttgctggcgg atctcctcac catgcaggag catttacccg gtaaagcatt caacgaaatg
     1981 acgctggtct atgcaggtga cgcacgtaac aacatgggca attcgatgct cgaagctgcg
     2041 gcgcttaccg gtctggattt gcgtctggtc gcgccacagg catgctggcc ggaagctgcg
     2101 ctggttacgg aatgccgcgc cctggcacag caaaatggcg ggaatattac gctgacggaa
     2161 gatgtagcca aaggagttga aggtgctgac tttatctata ccgatgtatg ggtgtcgatg
     2221 ggggaagcaa aagagaaatg ggcagaacgg attgcattac tgcgtgatta tcaggtgaac
     2281 agcaagatga tgcagttgac cggtaacccg gaggtcaaat tcctccactg cctgcccgcg
     2341 tttcatgacg accagacgac gcttggtaag aaaatggcgg aagagtttgg cctacatggc
     2401 ggaatggaag tgactgatga ggtcttcgaa tctgccgcca gcattgtttt tgatcaggcg
     2461 gaaaaccgca tgcataccat caaagcggtg atggtcgcaa cgctcagtaa ataattgata
     2521 tcgctataaa gatgccggga aatacaaaaa catcccggca tcacattatt ttatgcagta
     2581 atagttcaat tattccaccc caattttttt attgctatac gtaaatcttc cggaggattt
     2641 aatttttcta actgttcttt attaatatat tcacatatat ttattttccc atcccggagg
     2701 atttccatca ttcttgttct aatttttaat tcattaccca tcttttcttc aaaaatctca
     2761 acgacataac ccggattgtt atttacaatt tcatttgttt tttcttcatt accataaaca
     2821 tatcctaact cattaagata cagctgttcg ccctcatact tcttaagtaa ttgatagaag
     2881 taccacttcg caaaatcagg ttgcttcatg aaaaaatcct tcaaaaaaaa tatcatttac
     2941 taaaatgctg acaggcactt cattaaatag caacttaaca tcatcaaata accttagtat
     3001 taagcgtgat ttttcttgat tgcttaagtt aaaatcattg acatatgcaa ccaacttatc
     3061 ccaaatcaat tcctgtaatt tacggct
//
LOCUS       DAJTMK010000283         3061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTMK010000283 DAJTMK010000000
VERSION     DAJTMK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.290"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            35..796
                     /locus_tag="OO135_004074"
     CDS             35..796
                     /locus_tag="OO135_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR5399679.1"
                     /translation="MNNILLPPINIPCTLFETISLFDYFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFS
                     FFGQNKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNNFEDSFNGLGISVHNISAQKISLLSFQKYA
                     IGWSATIHFVAQDHSGLDVTDIKNKTYSKYRFFRI"
     gene            800..883
                     /locus_tag="OO135_004075"
                     /pseudo
     CDS             800..883
                     /locus_tag="OO135_004075"
                     /inference="COORDINATES: protein motif:HMM:NF023120.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            880..1371
                     /locus_tag="OO135_004076"
     CDS             880..1371
                     /locus_tag="OO135_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR5399680.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHRPWTDSDKINWYLKHQNEIKNQHPLPEGSWRTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVTNYLMIINEYLYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(1413..2363)
                     /gene="corA"
                     /locus_tag="OO135_004077"
     CDS             complement(1413..2363)
                     /gene="corA"
                     /locus_tag="OO135_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5399681.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2733..>3061
                     /locus_tag="OO135_004078"
     CDS             2733..>3061
                     /locus_tag="OO135_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399682.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTEWVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          930 a          673 c          621 g          837 t
ORIGIN      
        1 atattgagct taaatataac caaggcaaat gcaaatgaat aacatattac ttccgccaat
       61 taatattccc tgtacacttt tcgaaactat tagtttattc gattattttt ctgctgatga
      121 tatgcaatat ggtgatatgg tagaacaaga ctttctctca ctcggcctaa gcgatatctc
      181 agcaaaagtt gatccttaca gacttattaa ataccatttc cctggcccag gttctattaa
      241 tgttgctttt agtgcctcaa gttccggaac aaaaatatca caacgtgaat gcactgatat
      301 tttatttgca gaaatgaaag agctagcaaa aatgttttcc ttttttggac aaaataaaac
      361 acttatcgaa gatctgattg aacattttag atatggaaat ggcagcaatt ttcatagcca
      421 acaattaaat ttatcttttc atgaaaaaat aaataaatat ggttataata gtccaatacg
      481 aataatcaaa gaatgtatcg aaaatggcat taattcaact ccctcgacag gataccaacc
      541 tcttatcctg caaagcatta aaaccaaatt actttcatca agactaaata aatttaataa
      601 cttcgaggac agttttaatg gcttaggaat cagtgttcat aatatttcag ctcaaaaaat
      661 atctctttta agttttcaaa aatacgctat aggatggagt gcaacaattc attttgtagc
      721 acaggaccac tccggtcttg atgtaactga tatcaaaaac aagacttaca gcaaatatag
      781 attttttcgc atatagttct ttttacaacg gcataaagac tttgctttca aacctttttt
      841 caccaatttt aacactatcg aaagaatcga aaattatcta tgaaaagcaa actccagaaa
      901 attatattat gtttatttct tctatgctgc atctataatt tatggacatt gcgtcctgta
      961 cagattttgt atacatactc tgatgctggc aattcagtct ttctggtcgt tgatcatcga
     1021 ccatggactg acagcgataa aataaattgg tatctgaagc atcagaatga aattaaaaat
     1081 caacatcccc taccagaagg tagttggcgt acctggtatg tcattgatat cggtaatggc
     1141 ttcactgact ataaaaaata tatcgaaggg ccgtatgaag atctctactg tttcccgaca
     1201 attaagagca atgataattg catcgtgaca aattacttaa tgattattaa tgaatacctt
     1261 tatcgtaaca ctcatattgg tattaacgat tttactgagt atcaattaac acaagaaaat
     1321 aaaatagagc gagttttcaa tccgcatgac tttaaaaaag acaattttta gaaataagcc
     1381 ggataaaaaa atagctttat ccggcactca tattacaacc agttcttccg cttaaagtac
     1441 agatacggtg ccaggcccgc gaggatcata aagataatcg cgccagggta gccgaagctc
     1501 cacttcagtt ctggcataaa ctcaaagttc atgccatagc tggaagcaac gagcgtcggc
     1561 ggcaggaata ccacggatac caccgagaag attttgatga tgcggttctg ctcgatgttg
     1621 ataaaaccca tcgccgcctg catcaggaag ttcaccttct ggaacagaga ttcgttatgc
     1681 ggcagcaggg attcgatatc tcgcaggatt tcacgcgcct gctccagttg tccacccggt
     1741 aagcgtgctt tacgtaccag gaagttgagc gcgcgctggg tatccatcag acacaggcgt
     1801 actttccagc cgatatcttc cagttccgcc agcgtggaga gcgcctcatc gtactcatcg
     1861 ccctgatgcc cttccataat cacccggctc aactgctcca ggtcgctata gatattttca
     1921 atttcatctg ccaactgttc gattttggtt tcgaacagat ccagcagcag ctcgtaggcg
     1981 ttaccgtcta ccatcgactg gctacgggca cgcatacgat acagacgaaa agcgggcagt
     2041 tcacgctcac gcagagtaaa cagacgacca tcacggatgg taaatgccac agtggagtta
     2101 ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt gaatatgcag gccgtcgtcg
     2161 tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt ccgggcgggt tgccaggctc
     2221 tggccaagtt cagattgtac acgcagtcgc tcgtcgtcgt ccggttcgac aagatcaatc
     2281 catactgcat ttacaagggg ttgtgactct tcgacttcca gccgggtcag tcggttattt
     2341 tccagttgaa atgcgctcag catgaccggg actcccaatg cttaaaatat cggacagttc
     2401 ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa cagcctgact cagcgcgacg
     2461 ggaaaaattg aggtcgctga caactcctaa ggctatcagc aaaaagggat agccttagga
     2521 gttgatcctg gatgacagga tagtgagcca gtatctactg ggtgtgtcca aggcgaatgt
     2581 cctcttagaa tgatcgtgcg cgcatgttac gccagcaaaa ttttgccgtc aacacgcaac
     2641 agaacaccac agaacaatag ttgaacttcg cgctttttgg tactcgtcaa ggtcgctaac
     2701 aggataaaaa aatcccacat ccaggaccta taatggcgca tcggctactc attggtaaag
     2761 gaatgatcac cttgaatctc aaacggattt ttctcaccct caccttactt cccttgtttt
     2821 ctgttgccgc tgatgattgc gcactctcag atccgacgct gaccgtacag gcgtataccg
     2881 ttaatcctca aacagagtgg gtgaaaatgt actggcaaaa agccaatggc gaagcgtggg
     2941 gaacgttaca tgctctgctg gcggatatta atagtcaggg tcaggtgcag atggcgatga
     3001 acggcggcat ctatgatgaa agctatgcgc cgctcggttt gtacatcgaa aacggtcagc
     3061 a
//
LOCUS       DAJTMK010000284         3022 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTMK010000284 DAJTMK010000000
VERSION     DAJTMK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.291"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            207..1820
                     /gene="mppA"
                     /locus_tag="OO135_004079"
     CDS             207..1820
                     /gene="mppA"
                     /locus_tag="OO135_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5399683.1"
                     /translation="MKHFVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVANQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDMDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(1871..2902)
                     /gene="ynaI"
                     /locus_tag="OO135_004080"
     CDS             complement(1871..2902)
                     /gene="ynaI"
                     /locus_tag="OO135_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5399684.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          871 a          674 c          712 g          765 t
ORIGIN      
        1 cgtgcgaagc ctgaacgtag tgttcggtgt tctatgtgct ttgccgcatt ttggcgtcga
       61 ttatttacac taaacctgat ttaatatatt gatacataaa acattttatg cttcctgtgt
      121 cacttttttg atggaagttg tttgcatttc tttaaggcga aacaaataat tacgcatcaa
      181 ttttaatgtc ggttagaggg aaacttatga agcactttgt ttcagtcacg tgttgtgcgc
      241 tgttggtcag cagcatttct ctttcgtatg ctgcagaagt tccgagcggc acagtactgg
      301 cagagaagca ggagctggtg cgccacatta aagatgagcc tgcgtcgctg gatcccgcta
      361 aagccgtggg cctgccagag attcaggtca ttcgcgatct gtttgaagga ctggtgaatc
      421 agaacgagaa aggggagatt gtccccggcg ttgcgaatca gtggaaaagt aatgacaacc
      481 gtatctggac ttttaccctg cgcgataacg caaaatgggc ggatggcaca ccggtaacgg
      541 cgcaagattt tgtctacagc tggcagcgtc tggtggaccc gaaaacgcta tcgccatttg
      601 catggtttgc cgcgctggcg ggaatcaaca acgcacaggc gattattgat ggtaaagcta
      661 cgcctgacca gcttggcgtc accgcagttg atgcccatac cttgaaaatt cagcttgata
      721 aaccgttgcc gtggtttgtg aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag
      781 ccaacgtaga aagcggtaaa gagtggacga aacccggaaa tctgatcggc aatggcgctt
      841 atgttcttaa agaacgcgta gtcaatgaaa aactggtcgt ggtaccgaat acccattatt
      901 gggataacgc caaaacggta ctgcaaaaag tgaccttcct gccaattaat caggaatccg
      961 cagccactaa gcgttacctc gcgggggata tggatatcac cgaatccttc ccgaaaaata
     1021 tgtatcagaa gctgttgaag gatattccgg ggcaggttta tacgccgccg cagctcggga
     1081 cctattatta tgcgtttaac acgcaaaaag ggccgacggc agatcagcgc gttcgcctgg
     1141 cattaagtat gacgatagat cgccgcctga tgaccgaaaa agtgttggga acgggcgaaa
     1201 agccagcgtg gcattttaca ccagatgtta ccgccggatt tacgccggaa ccttcgccgt
     1261 ttgaacaaat gagtcaggaa gaactgaatg cgcaggcaaa aactttgttg agcgcagctg
     1321 gttatggtcc gcaaaaaccg ctgaagctga cgcttttgta taacacttca gaaaaccatc
     1381 aaaaaattgc gattgctgta gcatcgatgt ggaaaaagaa ccttggcgta gatgttaaat
     1441 tgcaaaatca ggaatggaaa acctatatcg atagccgtaa caccggcaat tttgatgtta
     1501 tccgcgcctc atgggtgggg gattataatg aaccctccac tttcctgaca ttattaacgt
     1561 caacgcattc aggaaatatt tcacgcttta acaatccggc atacgacaaa gttctggccc
     1621 aggcatcgac ggaaaatacc gttaaagcgc gtaatgccga ttacaacgcg gcagaaaaaa
     1681 tcctcatgga gcaagcaccg attgcaccaa tttatcaata taccaatgga cgattaatca
     1741 agccgtggct gaaaggttat cccattaata acccggaaga tgtggcgtac agccggatta
     1801 tgtatattgt gaagcattga tgtgatggga actggcgtta cccttgtgga taacgccagt
     1861 gatgtctgat ttagcgcccc tgttccggcg gtgtaatgtt atccatatac agcgtctggc
     1921 tggggaaggc aaagtccgcg ccgtgtgact gcacaatatc gataatcttc aaataaacgt
     1981 cttgttgtgc agcaagccat tcagcccata ccgtggtttt ggtaaagcaa taaaccataa
     2041 tattcaatga cgagtcggca aactggttga aataaaccag taaggtttgt cgctggtcga
     2101 tggccgggtg atttttcagc atctcacgta cggcttcgac aataacgccc acttttgccg
     2161 catcctcata acgtaaacca atggtcgtgg taatgcggcg gttggtcatt cgtcctgggt
     2221 tttctacgct gatcgacgaa aacagcgagt tcggtacgta caatggacga ttatcaaagg
     2281 tcgtaatttt ggtaattcgc cagccaattt ccgctactgt accttcgata tttctgtccg
     2341 gtgaacggat ccagtcgcca atactgaaag gacggtcgaa atagagcatg atcccggaaa
     2401 agaaattact cagaatatct ttaccggcca taccgacagc cagaccacca ataccaccaa
     2461 aggtcagcaa gccagaaagg ctcatgccga aatgttcgcc ataaagcaga acaagcacca
     2521 caataatggt gattttgata atgcgcgaca taatccgcgc actggtgata tcgcgacctt
     2581 ttttaatctg ctgtttttca aactgattaa tcagcaaaaa tagcttaatc gtcagaataa
     2641 ccgcaatcag ggacgtacag ataaaatcga taacgcctgg ggtgataaat ttgagtttat
     2701 agttttctat aacataatta ataatgctac caacagcact gataattatg gtgtagatta
     2761 aaaattgcac cgcatggaat aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa
     2821 agctcatcag aatcaatgct gcgcagctac cgaaaataat gaccagatta agcgcattat
     2881 ttgtaaacag ttcagcgatc attgttttat caggctcctc cagataattg tcgtcatgcc
     2941 ggaaccccct ggcggggcta ttttaccgcg acaattcatt cagatcatca atagtcaggg
     3001 aaggaagtag caacattagc ta
//
LOCUS       DAJTMK010000285         3016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTMK010000285 DAJTMK010000000
VERSION     DAJTMK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.292"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            31..972
                     /gene="zitB"
                     /locus_tag="OO135_004081"
     CDS             31..972
                     /gene="zitB"
                     /locus_tag="OO135_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5399685.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(969..1688)
                     /gene="pnuC"
                     /locus_tag="OO135_004082"
     CDS             complement(969..1688)
                     /gene="pnuC"
                     /locus_tag="OO135_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5399686.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(1726..2769)
                     /gene="nadA"
                     /locus_tag="OO135_004083"
     CDS             complement(1726..2769)
                     /gene="nadA"
                     /locus_tag="OO135_004083"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5399687.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     EFIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALEP
                     LNRMLDFAATLRG"
BASE COUNT          751 a          778 c          718 g          769 t
ORIGIN      
        1 taagtgagaa taattaaggg atgactcgtc atggcgcact cacactcaca cacgtcctca
       61 cacctgccag aagataataa tgctcgtcgc ttgttgtatg ctttcggcgt gactgccggg
      121 tttatgctgg tagaagtcgt tggtggtttt ctttctggtt ctctggcatt gctggccgat
      181 gcgggtcata tgttgaccga tactgccgcc ctgcttttcg ccctactcgc cgtgcaattt
      241 tcacgtcgtc ctcccaccat tcgccacact ttcggttggc taagactgac cacgctcgcg
      301 gcgtttgtga atgccatcgc cctcgtggta attaccattt tgattgtctg ggaggcgata
      361 gaacgcttcc gcacgccgcg tccggtcgag ggcggcatga tgatggcaat tgccgtggct
      421 gggttgctgg caaatatact ttctttctgg ttacttcatc acggcagcga agagaaaaac
      481 ctcaacgtac gtgcagcggc attgcatgtg ctcggggacc tgttgggttc ggttggggct
      541 attattgccg cgttgattat tatctggacc ggctggacac ctgctgaccc cattctctcg
      601 atagtggtgt cgcttctggt tctgcgtagc gcgtggcgac tgttgaaaga tagcgtgaat
      661 gaattacttg aaggtgcacc ggtatcgctg gatatcgctg aactgaagcg tcgtatgtgc
      721 cgggaaatcc cggaagtccg caatgtgcac catgtacatg tatggatggt aggtgagaag
      781 ccggtgatga cgctgcatgt gcaggtgatc ccgccacacg atcacgatgc cttgttggat
      841 cagatccaac actacctgat ggatcactat cagattgagc acgccaccat tcagatggaa
      901 tatcaacctt gtcatgggcc agactgccac ctgaatgaag gggtgtctgg ccattcacat
      961 catcaccatt aatgggacag cgcgcatgag cctctttcac gtgcgctgtt gatccacatc
     1021 cgactgccgt tgagcgcaat aaaggtcagg atgatgtact ccagagacat ggcgtaaacg
     1081 ccctgaagtg caaaaataac gacgctaatc acgttaataa tcacccacaa cagccagttt
     1141 tccacatact tacgcgtcat cagaagcatt gccacgatag ataacaccat catgcatgaa
     1201 tcccagaacg ggaaagcgtc cggttgcagt tcaggcatca caacctgtaa tcctaatgct
     1261 tgcatgatca tgactgccac gcgggtcaaa aatgcaaaca ccggattgat aaataccgtc
     1321 atcagaccaa tcgaaacaac gcaaaccgcc aaccagctga gcgccttcgg caatggtaac
     1381 cagcgaattt tcaactccgc ctcgttctga ctggtttgtc gcgaccacgc ataccaaccg
     1441 taaatattcg cggcaaagaa aaacacctgt aatagcaggc tggcatacag ctgaatctga
     1501 aagaaaataa tgccaaacaa ggtgacgttg atcaggccaa agaagtagtt gctgatcttc
     1561 tccagactgg caaggccaat acacagcaac ccggcgatcg tgcctaccgc ttcgatccat
     1621 gagagatcat aaccgcctgc tcctattggt atatgtacca ggatattctg cacactaaaa
     1681 aaatccatct ttcccccaaa gcgtaacgtt acgccttatt attcgttatc cacgtagtgt
     1741 agccgcaaaa tccagcatac gattgagcgg ctccagcgcc ctctctcgca gcctttcatc
     1801 aacatgaacc tcgtgattgc ttccttcctg ttctaatgcc tctgcgatgg cctgaaggcc
     1861 attcatggcc atccacggac aatgcgcgca gctgcggcag gttgcaccct cacctgcggt
     1921 tggtgcttcc agtaactctt tatctggcac cgcctgctgc attttgtaga aaataccccg
     1981 atcggttgcc acaataagcc tctgatgtgg caatgttttc gcagcagcga tcagttgact
     2041 ggtggaaccg accgcatccg ccatatcgac aatagcttgt ggtgattctg gatgcaccag
     2101 tatggcagca tccgggtatt cttcttgcaa gcgggttaac gcctgagtct taaattcatc
     2161 atgcacaata caggcaccct gccagcatag aatgtctcca cccgtctgtt tttgcacgta
     2221 acgccccaga tgtttgtcgg gtgcccagat gattttttca cccaaactat caagatgatc
     2281 aataaattcg acggcaatgc ttgaagttac cacccaatct gcgcgcgctt ttaccgcagc
     2341 agaagtgttg gcgtagacga cgacagtacg atcgggatgg gcatcgcaaa atgcgttaaa
     2401 ttcttcaaca gggcagccga gatccagtga acattcagcc tgaagtgtcg gcatcagaat
     2461 tgttttttcc ggactgagaa ttttggcggt ttctcccata aatctcaccc cagcgactaa
     2521 caaagtagaa gcgggatgct ttgcaccgaa gcgcgccatt tccagagaat cagaaataca
     2581 gccaccggtt tcttctgcca gttgttgaat ttcgggatcg gtatagtagt gggcaaccat
     2641 caccgcatta cgttctttta gcagacgttt tatcttctcg cggtaatacg ctttttcatc
     2701 aatgcttaac ggcgtcggct tcggggggaa aggataaatc gccgtgtctg gatcaaacat
     2761 tacgctcatc ttaccatctc gttttacagg cttaacgtta aaaccgacat tagcgtaata
     2821 ttcgctgtta tcggtgtaat ttgttttata tgctaaacaa gatagtcgaa tggtggggaa
     2881 aagtcactcg aattacgcct aattccaccg acgtagagtt gcttgcacag taagctctga
     2941 cgatgtcatc aggtttacaa agtgaaattg aggatgcctg aatagcaaaa aagcgccttt
     3001 agggcgcttt tttaca
//
LOCUS       DAJTMK010000286         3012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTMK010000286 DAJTMK010000000
VERSION     DAJTMK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.293"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..220
                     /locus_tag="OO135_004084"
     CDS             <1..220
                     /locus_tag="OO135_004084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5399688.1"
                     /translation="VRVAVVADVCGVSQRAIYKWMDNGKLPRTEYTGETNYAEKIALA
                     SNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            217..642
                     /locus_tag="OO135_004085"
     CDS             217..642
                     /locus_tag="OO135_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5399689.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKSYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            714..1784
                     /locus_tag="OO135_004086"
     CDS             714..1784
                     /locus_tag="OO135_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR5399690.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     IPQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQVMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDSEIPPGFRG"
     gene            1825..2247
                     /locus_tag="OO135_004087"
     CDS             1825..2247
                     /locus_tag="OO135_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5399691.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            2244..2549
                     /locus_tag="OO135_004088"
     CDS             2244..2549
                     /locus_tag="OO135_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5399692.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            2536..2991
                     /locus_tag="OO135_004089"
     CDS             2536..2991
                     /locus_tag="OO135_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5399693.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSDPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT          851 a          646 c          861 g          654 t
ORIGIN      
        1 tgttcgcgtt gctgttgtgg ccgacgtttg tggtgtcagc caaagagcaa tctataaatg
       61 gatggataac ggaaaattgc ctcgcacaga atataccggc gaaacaaatt acgctgaaaa
      121 aatcgctctt gcatcaaacg gattattttc tgccgatgca attttaacta ttggcaggaa
      181 taaaactact acgaaaaagc tgatgggagt tgattcatga aaatcaagca tgaacacatc
      241 cgcatggcga tgaatgcctg ggcgcatccg gacggcgaaa aagtaccggc tgcgaaaatt
      301 accaaatcgt atttcgagct gggaatgacg ttcccggaac tgtatgacga cagccatccg
      361 gaagccctgg ctcgcaatac tcagaaaatt ttccgctggg tggagaaaga cacccctgat
      421 gcgcttaaaa aaattcaggc gttgttacca gctatcgaaa aagcaatgcc acctctgctg
      481 gtggcccgaa tgcgcagcca tagctcagcc tattttcggg aactagtgga gacgcgggaa
      541 cgactggtga gagacgctga tgattttgtc gcagtggcga tcgctggttt caaccagatg
      601 aatcgtggtg gccctgcagg aaatattgtg gctgtgcatt gactcgcaat attcataccg
      661 gatcacttcc ggcaatttgt gagtaaaaag attcggtatc aaaagaggtg agtatggcta
      721 acgcctggct cagattatgg catgacatgc caaatgaccc taagtggcga acaattgcca
      781 gggtgtcagg gcagccaatt gcaacagtga tggcagtgta tatccacctc ctggtgagcg
      841 cgtcacgaaa tgtcacgcga ggtcacattg atgtcacgac agaagatttg gcaagtgcgc
      901 tcgacgtgac agaagaggta attgattcaa ttttgcagac gatgcagggg cgggtacttg
      961 atggtgattt aatcactgga tgggaaaaac gccaggtgct gaaagaggac aacggcaata
     1021 ttccgcaaac cgcaaaatct cctgcagagc gcaagagggc gcagcgagag agggaaagaa
     1081 agcgggaaca aaatggcgat tgtcacggcg cgtcacgata tgtcacgcac atgtcacgac
     1141 gagtcacgac agataaagat acagataaag atacagatca agaagatcaa aacactatgg
     1201 tccatggcgt aaaaaacgcc acgaaccagg caggggatgt tcagaccgtc aatcctggtc
     1261 agccagcagg cacgacaccg gaagccgatt cagcgtatgc gctgaaagcc gattcgggcg
     1321 ctgtgcagca ggtgatgacc gcaagtccgg agcaatcaca ccaactgcag cagcctgaag
     1381 ccgattccgc cattcagcgg gaagccgatc gggtagtccc ggaaaacacc gggcagtctg
     1441 tgggacgagt ggattatccg gatgtgttcg aacaggtctg gcgggagtac ccgttgcgtg
     1501 ccggggcaaa cccgaagaaa tccgctttca gtgcctggaa ggccagatta cgcgaggggg
     1561 tgccaccaga ggccatgctg gatggcgtga ggcgttacgc aagatacctg gcggctaccg
     1621 ggaaaacggg aacggaattt gttcagcgag cgacgacgtt ttttggaccg gaccggaatt
     1681 ttgagaaccc ctggttgctc ccggtaagcg gcacgaacaa ccagcgttgt gtgaatcata
     1741 tttctgaacc ggatagcgaa attccgccgg gcttcagggg gtaagtgtta atttctggtc
     1801 atgaggtaat tttcaggagg gcttgtggca aaagttttta cacaagaaga gcgggaaaaa
     1861 attaaagggc aggttcttga actcgtacgc cagagtgggc gcgagacgtt acgacaactg
     1921 gaagctaaaa ctggggcaac aagatatctg atgagcgttc tggccagaga gctggttgcc
     1981 agtggcgatg tatacaactc tggttacggg ttattcccgt ctgaacaggc gcgtaaggac
     2041 tggcaaaatg cccgtaaaaa gctctcaagg gcaaagctga agaaaccatc tgcggttgat
     2101 ccggacctta tctggtcatt acctgacgga gaaatacgtc gctacgacag tcgcctaaac
     2161 ataatctgtc gcgagtgccg gaagagcgaa gttatgcagc gcatattgtc gttttatcag
     2221 ggtaattttc aggaggtgat ggcgtgaggg tgagagtcta tatcgccggt ccaatgaccg
     2281 ggtataaaaa tttcaaccgt gaggcgtttc acaatgcgga agaggaactg aaacgggaag
     2341 ggcataccgt cttaaacccg gcagtacttc cggacgggct gacacagccg cagtacatgg
     2401 atatctgcat ggcgatgatt cgttgtgtgg atgcgattta catgctgaaa ggctggcagc
     2461 ggtcagcagg cgctaaggca gaactggcgc tggcggagaa actggggcat gcggtgattt
     2521 ttcaggagac agccagtgtg caagactaat taccaggcat tacgtgaacg ttattcacca
     2581 attcaggtgc cggaatgtcc tgtttgtggc gatgaaatgt cgatacagcg catattttcc
     2641 agaacgcata ttgtgtatgc ctgcacaggt gagggggatg atggatattt taaaactggt
     2701 cgaacttttg cggatgagca ttacctgaaa tcgcgcgtaa ccgtcgttga tgttagcgat
     2761 cctgacgtac tggctttact gaaagagctg gaagttaaag acaaacgcat tgcagaattg
     2821 acagatgcgc ttacacaaat gattaatgcg cacaaaacca caattcgttt tggtcatgaa
     2881 cgcataactg aatgtggtgg tgattgcgac tcgccggaaa agatgatttc agaaaatccg
     2941 gatatcagaa tggcagaggc tgttttgaga gcaggaataa aaactgaata attaaattta
     3001 gcagtaagcg tg
//
LOCUS       DAJTMK010000287         2962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTMK010000287 DAJTMK010000000
VERSION     DAJTMK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.294"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(57..897)
                     /locus_tag="OO135_004090"
                     /pseudo
     CDS             complement(57..897)
                     /locus_tag="OO135_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(1319..1471)
                     /locus_tag="OO135_004091"
     CDS             complement(1319..1471)
                     /locus_tag="OO135_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5399694.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
     gene            complement(1674..2081)
                     /locus_tag="OO135_004092"
     CDS             complement(1674..2081)
                     /locus_tag="OO135_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399695.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2158..2386
                     /locus_tag="OO135_004093"
                     /pseudo
     CDS             2158..2386
                     /locus_tag="OO135_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            2370..2792
                     /locus_tag="OO135_004094"
     CDS             2370..2792
                     /locus_tag="OO135_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399696.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
BASE COUNT          762 a          652 c          738 g          810 t
ORIGIN      
        1 tgagggcagg tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat
       61 tttaaggaaa gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc
      121 cagccctgtt tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca
      181 ggaacggttt cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga
      241 ccatcacccg gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc
      301 atgcggtcga actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag
      361 cccatcacca ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg
      421 cggtgttcga atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgagg
      481 gattctattt tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata
      541 gaagtagtga cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc
      601 ataatgacat cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg
      661 gtggtgagac gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc
      721 tgccccgcac gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaaccgc
      781 ttcagcgcgg ttatgcgctc gagtaacttt cggggtgcta atcccagcgc ccgttttatg
      841 aggtgcaggt gcattataat tccacccgtt ctggttttca atagctacat tcaacatttt
      901 ctctttcctt tcatcaccga agtgaatttt gtgatgcggt gcctagtgcc tccaggtgac
      961 gttaaccagt taacaattac agtcggcttt cccacccaaa ccaataagga ctaacatgac
     1021 ttttaactgt gccgcgtgcg cttagccgca ttcaccgcat cacaaaattc actttaaaaa
     1081 gggcggacat cagccagcaa ttaaaccgat gccgccaact ggtacttcac acagcaatgt
     1141 cgttatttac aaccggaagc gcactcccac catttaaatt tcacagacaa gaccgactct
     1201 ttatgaatac cggaaatgcg ccttcgtgtt gtgcccggtt ttatttcacc acctccgggc
     1261 tttggtggcc tcggctatac ctctacagcg agaatattga attaatccaa taaatggatt
     1321 agctggtatt tttggcaagc cagcgacgtg cgccagcttc ggttttaaac gttttgcttt
     1381 tggtatacgt catggcggtg aatgtgccgt cctgattggg aaacacgcca cacactaggg
     1441 attcgttgtt gccaagatcg atagtatcca tgctgacctc atttcccctt aacgccgggg
     1501 tagcggaact gtttgctgag aacaccgtgc ggtgtcttga tgaatgaaat ttagaataac
     1561 ctaaggtgtt gggttaagat tttatgtaga aaaacctaag tttttttgat gtaaaaaaca
     1621 caagtgtttg aaagtttgtg ctttttatta cagggtgtgg agaaaaaagg ggattatttg
     1681 tttgcgcttc ttttgcgagc tttgagtagt tcttcaaaaa gtttgttgaa attttcaact
     1741 cgagcacgca tctctgacaa cagggccttt tgctctgact caggcagtgc gtcgaacagt
     1801 tgaagtaact ctttttgatc ttctgtcaga atggctggct gattatccgg gatcggttcg
     1861 cctggttgtt tatcttcatc cccaaaaaga agccaagtcg gtgagcactg aagcgcttgg
     1921 ctcagtgcga ataatctttt ccccgccggc tgtgtttcat ctctttccca ttgagaaatt
     1981 gttacgtgag ccactttgac cagcttacct aatgcggcct gagacagttt taatttttta
     2041 cgcctatgta agaggcgagc accgaaggtt tcgtttttca tattagggaa ttctaatttt
     2101 tcttgactta ggtttctcta cgagctagtt tccttaggaa aatctaagga gctcgatatg
     2161 ttgaaaattg atgctatagc gttttttggc agcaaaacaa agcattgcca atgccgcagg
     2221 agttaggctg gcaagcattg ctgcatgggg ggaactggtt cctgaaggtc gcgcgatgcg
     2281 cctgcaagag gcatctggcg gggaacttca gtacgacccc aaagtttatg acgaatatcg
     2341 taaggcaaaa cgacctggga aggtgattca tgaaaatcag gcatgagcac atcgaatcag
     2401 tgctgttagc cctggcagcc gaaaaagggc aggcgtgggt cgctaacgca attactgaag
     2461 aatatctgcg gcaggggggc ggcgaattgc ctctggtacc aggcaaggac tggaacaatc
     2521 agcagaatat ctatcaccgt tggttgaaag gtgaaacgaa agcgcaaagg gaaaaaattc
     2581 agaaactgat ccctgcggtt ctggcaattc ttccgcgtga gctgcgtcac cgactctgta
     2641 tcttcgatac cctggaacgc cgtgcattac tggcggcgca ggaagcgttg agtacggcaa
     2701 ttgatgcgca tgatgacgca gttcaggccg tttaccggaa agcacatttc agcggtggtg
     2761 ggtcgtccga cgattctgtc gttgtgcatt gatgtttatg ccgaacctcc tctggttctg
     2821 ttgattgggg aatcacagat tatatccgga ggaaggttcg gcaccagacg aggtttctat
     2881 atatgagcat gaaactaatg gcaaaagcaa tggaaattaa agtgggtaat ccattgcgta
     2941 agctggttct gattaagctg gc
//
LOCUS       DAJTMK010000288         2944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTMK010000288 DAJTMK010000000
VERSION     DAJTMK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.295"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            579..1295
                     /locus_tag="OO135_004095"
     CDS             579..1295
                     /locus_tag="OO135_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399697.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFLRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1847..2251
                     /locus_tag="OO135_004096"
     CDS             1847..2251
                     /locus_tag="OO135_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399698.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2314..2944
                     /locus_tag="OO135_004097"
                     /pseudo
     CDS             2314..2944
                     /locus_tag="OO135_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          830 a          605 c          665 g          844 t
ORIGIN      
        1 gattgtgggc cataaatatt ccaaaaaaat agcccgctcc gtttattacc ggaacgggcg
       61 agagcatgac attgtctgta attataatga gactgctgat tctacttatt tttaatttca
      121 agaaaagcgt tttctacaaa gaattatgag atttaatgtt ttgtttagac aatattatca
      181 ttctgttcaa taatgaaagt cttcatcaac aacaagtaaa aacaacgtcc ctctcatcga
      241 accggacttg cagatttccc gcatccggct ctcttgcaac acatcagacc ttcacgttcg
      301 ggcggttatg gggaaggtcc agcaagctgt atgagcccta actgtttgaa taccagtaga
      361 ttaccagaaa cttctatcga ttgaagacat taccaactag cgttactaaa tgttgaccaa
      421 gttttgttca cttgtctggt tttgatagta gcattctgca gagtattttg atatgataaa
      481 tatgcattaa caatgatatt attgttaatt tactagcatg aaaattaaaa gatcaacttt
      541 ttatctcgaa tattttttac attatcagtt ggtttcttat gaatgacaat tcattgctta
      601 gaaattcttc attattcatc gcctatatgg gatgtgtagg gtgggtcagt gcttattctt
      661 atggctgggg aacctctttt tactatggat ttccatggtg ggttgttggt gcaggtttgg
      721 atgatgttgc cagaagttta ctctatgcta tcatagtaat gggtatactt tttaccggtt
      781 gggggattgg tattttattt tttcttctta taaagaaaag aagtaaaata caagacttga
      841 gttttttaag actatttttt gctattaccc tactgttttt ccctgtaata ttcgaacttt
      901 taattctcaa acaatatttc atacttccat tatcactttc atgcattatt agttcattgg
      961 ttatttcaat aataattcgc atatatggac ggatcttttc tgtatcatgt ttttcagata
     1021 tcccttttgt tagagaacac cgcattaagt taattatggc tggttttttg gtttattttt
     1081 ggcttttttc ctttcttgtt ggctggtata aacctcaatt aaaaaaagaa tatcagatgc
     1141 tttgttataa taatagttgg tattatgttc ttgctcgtta tgattcacgg ctcgttttgt
     1201 ctagttcatt taaagatgac agtaacagat tcttaatatt taatacagaa cagtcgggat
     1261 tttatgaaat caatgatgtt tatgtcagaa agtaataatc ctctcccaca aagtgaatta
     1321 aacgaatata taatgcgttc gaagtatatg tattgagctg tcatactgag aaacaaacct
     1381 tagggcagca caattaaagc cagaaacaac tatgcaagaa agttactaaa taagtgctcg
     1441 gaacagtaaa acactcaggg gggactcagt ccagattatg taatcagata aattgccaaa
     1501 catcacaaat caccagcagg actgagtgat gccgagcatt gcaccaatac cccgtgatga
     1561 acgatgcctg atgcagaaag ctccctataa aacaaagaac aaaaactacg accggcgatt
     1621 caccgtcata ctgatgctgc atcgtggtga cactgtctgt cactttcatg ttgactgaac
     1681 aactctgctg cgaagaaggt gtaccgccgg cgggatttga ttgtccggcg tctagcttcc
     1741 acttccctga tggaactgaa agttgttatg gccggtcagc ttgccctgtc cgcaggacat
     1801 gaggtgctgt gacggacagc accagtaacg gaaagtgaca ataaaaatga atactgcgct
     1861 gatagttgca ctaatgtgta tgtggtatgc ggtacccgct gctgcaaaag agactttact
     1921 ggctatgcca cgtaattcaa cagaacattg ctatgcggag attaatgtgc acggaccata
     1981 tggggtgtat ttccgagtgg tcccgcatcc ccccggggga aagagctggg tcgaatgtaa
     2041 cagtgactac tattactctg ataaaccacc gggagtacag atactcggaa cccgtgcagg
     2101 gtgtcgggta tacggaatct gcgggaccac ctcgacactg catgtggctg gaagaggggt
     2161 tgtgtgtatt aaaaatatat gcagtccacg tgggatgata attcacagga tacggaaacg
     2221 tcctgtagtg gctgtaagcg atgagatgtg atcttaccca gcaatagtgg acacgcggct
     2281 aagtgagtaa actctcagtc agaggtgacc cacatgacaa aaacagtatc aaccagcaaa
     2341 aaaacacgta aacagcattc gcctgaattt tgcagtgaag ccctgaagct tgctgaacgc
     2401 atcggtgttg ctgccgcagc ccgtgaactc agcctgtatg aatctcagct ctatgcgtgg
     2461 cgcagcaaac tgcagcaaca gatgacctcg tctgagcgtg aaagtgaact ggccgctgaa
     2521 aatgcccgcc tgaagcggca actggcggtg caggcagagg aactggctat cctccaaaag
     2581 gccgcgacat actttgcgaa gcgcctgaaa tgaagtatgt ctttattgaa aaacatcagg
     2641 ctgagttcag catcaaagca atgtgccgcg tgctccgggt ggcccgcagc ggctggtata
     2701 cgtggtgtca gcggcggaca aggataagcc ctcgtcagca gttccgtcag cactgcgaca
     2761 gcgttgtcct cgcggcggcc tttacccggt caaaacagcg ttacggtgca ccacgcctga
     2821 cggatgaact gcgtgctcag ggttaccact ttaacgtaaa aaccgtggcg gcaagcctgc
     2881 gccgtcaggg gctgagggca aaggcctccc ggaagttcga cccggtcagt taccgcgcac
     2941 atgg
//
LOCUS       DAJTMK010000289         2939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTMK010000289 DAJTMK010000000
VERSION     DAJTMK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.296"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..408
                     /gene="speG"
                     /locus_tag="OO135_004098"
     CDS             <1..408
                     /gene="speG"
                     /locus_tag="OO135_004098"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR5399699.1"
                     /translation="IHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQ
                     GKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFIN
                     GQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(411..1121)
                     /locus_tag="OO135_004099"
     CDS             complement(411..1121)
                     /locus_tag="OO135_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5399700.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1229..1534
                     /locus_tag="OO135_004100"
     CDS             1229..1534
                     /locus_tag="OO135_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5399701.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1733..2939
                     /locus_tag="OO135_004101"
                     /pseudo
     CDS             1733..2939
                     /locus_tag="OO135_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          784 a          731 c          679 g          745 t
ORIGIN      
        1 attcacgatc agagcgaacg acgctttgtg gtggaatgtg acggcgaaaa agccggtctg
       61 gtggagctgg tggaaattaa ccatgtacat cgccgggcag aatttcagat aattatctcc
      121 ccggagtatc aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc
      181 ttcaccgttc tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg
      241 attcacattt accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt
      301 attaatggtc aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca
      361 gagcacaaaa caccaggtca gactctcctg aagccgaccg cacaatagca ttaataataa
      421 tcgatcgtat ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca
      481 atgatcagct gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca
      541 ttaccaactc gttgattatt cagtaaagta accgctgtgt aatctaattt tttgatcggg
      601 tccgttgatg gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc
      661 agtggataac cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta
      721 atcacgaagc cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc
      781 tggcattttc cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg
      841 gcgtccagta ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca
      901 aaacagcctt cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc
      961 attaatgtct gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta
     1021 gagaaactgg ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg
     1081 aggaatatcg cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag
     1141 agatgattct caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa
     1201 gaagttatct tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct
     1261 gctcgccctc gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag
     1321 cgatatatca cagcgcatta tcaataatgg cgtacccgaa agcagcttca cattaagtat
     1381 tgtacccaat gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gtgctaatga
     1441 tacgcataaa attctctaca cccgcaccac cagcgctaac gtctctgctc ccgcgcagtc
     1501 tacccaggat ggcgcgcctg ccgaaccgca gtaatacatt atcgactgaa cgccggatat
     1561 gacacatccg gcgatttgaa catacaacat aatcccacct tattactcat acccttctat
     1621 tgatgtggat taataattat taacccaaaa tgggtagacc tcctctattg ttagcgcgct
     1681 aaatattcaa tacataaaca tttatataac gataaagaac agggagtgag ttatgtccaa
     1741 aaatgaccga atggtgggca tcagccgcag aacactcgtt aaatcaacag cgataggttc
     1801 tctggcgctg gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcaac
     1861 ggtacaacag gccagcgaaa aagtgatctg gggtgcctgt tccgtcaact gtggtagccg
     1921 ctgtgcactt cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac
     1981 cggcagcgac gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg
     2041 ccggcgtatt aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg
     2101 tgaaggcaaa ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct
     2161 gaaaaaaacc gtcgaacaat atggcaatga ggctgtatat attcagtact cttcggggat
     2221 tgttggcggc aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa
     2281 ctgctacggc ggttcactca atcagtatgg ctcctacagc accgcgcaaa tctcctgtgc
     2341 catgccctac acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa
     2401 gctggtcgtg aagatgaatg gcttcctatt cgcccgggca ccgatgccgc gctggttgcg
     2461 ggtattgcct gggtattgat taacgaaaat ctcgttgatc aaccttttct cgataaatac
     2521 tgcgtcggct atgacgaaaa aaccttaccc gcagatgcac caaaaaatgg tcactataaa
     2581 gcctatattc ttggtgaagg tgatgataat acagcgaaaa cgccgcagtg ggcttcgcaa
     2641 attaccggta tcccggtgga ccgtatcatc aaactggcgc gtgaaattgg cacagcaaaa
     2701 cccgcttata tctgccaggg ctgggggccg caacgccagg caaacggcga actgactgca
     2761 cgcgctattg ctatgttgcc tattttgacg ggcaatgtcg gcatcagcgg cggaaatagt
     2821 ggcgcgcgtg aatcgaccta taccattacc atagaacgcc tgccggtgct ggataatccg
     2881 gtcaaaacgt caatctcctg cttcagctgg acagatgcta tcgatcatgg tccgcaaat
//
LOCUS       DAJTMK010000290         2935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTMK010000290 DAJTMK010000000
VERSION     DAJTMK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.297"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            102..221
                     /locus_tag="OO135_004102"
                     /pseudo
     CDS             102..221
                     /locus_tag="OO135_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            471..1337
                     /gene="ospB"
                     /locus_tag="OO135_004103"
     CDS             471..1337
                     /gene="ospB"
                     /locus_tag="OO135_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5399702.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKSVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1665..2405
                     /gene="phoN2"
                     /locus_tag="OO135_004104"
     CDS             1665..2405
                     /gene="phoN2"
                     /locus_tag="OO135_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5399703.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2765..2860)
                     /locus_tag="OO135_004105"
                     /pseudo
     CDS             complement(2765..2860)
                     /locus_tag="OO135_004105"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          957 a          543 c          564 g          871 t
ORIGIN      
        1 agactggccc cctgaatctc cagacaacca gtatcactta tttaagtgat actggttgtc
       61 tggagattca gggggccagt ctagcagcgg taagagaagt tcatcatact tggcgcagtt
      121 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
      181 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
      241 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
      301 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
      361 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
      421 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
      481 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
      541 catttgcaca tataataaaa agcgtaaaaa attcatcatg tactcaccca aaagcagcat
      601 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
      661 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
      721 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
      781 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
      841 aaataaaaac agtaaacgac actgatttta aagagtataa taaggtttat attcttgggc
      901 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
      961 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     1021 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     1081 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     1141 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     1201 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     1261 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatca
     1321 ataaagaact ggattagtat aaaaatttta acctatagtt aattgtttgc atttaagatg
     1381 aagttatttt ttctttagta gaaaggtgct ccctatgagg gaaaatcaca aatcatcata
     1441 atacagagac aaaacgatac gaacaaatag ataaaaaaca tcattctttt accacattat
     1501 ttttcgtaaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     1561 atcactctgt tcaaattttt ccgcatgact tgtgtttttt gtaacacagc tcgtttttta
     1621 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     1681 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     1741 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     1801 cggcagagga ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     1861 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     1921 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     1981 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     2041 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     2101 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     2161 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     2221 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     2281 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     2341 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     2401 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     2461 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     2521 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     2581 acgatgatct ggcaacctga gttcacagat aaaacgttct ctaggaaact cggggcggtt
     2641 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     2701 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     2761 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     2821 aatctttgct tttcattgca ttgtcaagca tagtgtccgt aagcgtacag cctgaaccgt
     2881 ctggtcagaa tctgacgaat tagacaaagt ggtgtccacc aaataagtag tggga
//
LOCUS       DAJTMK010000291         2924 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTMK010000291 DAJTMK010000000
VERSION     DAJTMK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.298"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..345
                     /locus_tag="OO135_004106"
     CDS             <1..345
                     /locus_tag="OO135_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR5399704.1"
                     /translation="RKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGER
                     SCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRK
                     DIMRRLTQDVCE"
     gene            complement(411..2789)
                     /gene="ppsA"
                     /locus_tag="OO135_004107"
     CDS             complement(411..2789)
                     /gene="ppsA"
                     /locus_tag="OO135_004107"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5399705.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSDVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKVLLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          707 a          798 c          724 g          695 t
ORIGIN      
        1 cgcaaaaaag atattattgt ccgcggcggc gaaaatatta gcagccgtga agtggaagat
       61 attttattgc agcatcctaa aattcacgat gcctgtgtgg ttgcaatgcc cgatgaacgt
      121 ttaggtgaac gatcatgcgc ttatgtcgtg ctgaaagcgc cgcatcattc attatcactg
      181 gaagaggtgg tggctttttt tagccgtaaa cgggtcgcaa aatataaata tcctgaacat
      241 atcgtggtaa tcgaaaaact accgcgcact gcctcaggta aaatacaaaa gtttttgtta
      301 agaaaagata ttatgcggcg attaacgcag gatgtctgtg aatagattga ataagtttca
      361 tcttcgggga tcacataaac ccggcgacaa aacgccgccg ggtatttatt ttatttcttc
      421 agttcagcca ggcttaacca ggtttgcacc acggtgtccg gattcagaga caggctatcg
      481 atcccctctt ccatcaacca tgcggcaaaa tcttcgtggt cggacggacc ctgaccgcaa
      541 atcccgacat atttgccctg tttcttcgcg gcacggatag ccatcgacag caatactttc
      601 accgcatcgt tgcgctcatc gaacagttca gacaccacgc cggagtcacg atccagaccg
      661 agcgccaact gcgtcatgtc gtttgagcca attgagaagc cgtcgaaata ttcgaggaac
      721 tgctcggcca gcaaggcgtt ggacgggatt tcacacatca tgatgatttt cagcccgttc
      781 tcaccacgct tcagcccctg acgcgccagt tcttcaacca ccgctttcgc ctggtctacg
      841 gtacgcacga acgggatcat gatctcaacg ttggtcagcc ccatgtcgtt gcgcacacgt
      901 ttcactgctt cacactccag cgcgaagcag tcgcggaagc tgtcggaaac atagcggccc
      961 gcgccacgga agccgagcat cgggttctct tcatctggct cgtaacgctc accaccgacc
     1021 aggttggcat attcgttcga tttaaaatca gagagacgga caatgacgcg cttcggataa
     1081 aacgcggcac ccagcgtcgc gatcccttca gtcagacgac caacgtaaaa ttcacgcgga
     1141 gaatcaaaac ctttcatcat ctcgcggatt tcgttttgca actgcggttc ctgatcgtca
     1201 aactcaagca gtgcgcgtgg gtggacgcca atcatacggt tgatgataaa ttccagacgc
     1261 gcaagtccca cgccttcgtt cggcagacag gcgaagtcga aagctcgatc cgggttaccg
     1321 acgttcatca tcactttcaa cggcagatcc ggcatggttt ctacgctgga gcttttcacg
     1381 ctaaattcca gcaactccgc atagacgtaa ccggtatcac cttcggcaca agaaacagtg
     1441 acgttctcac cgtctttcat ccgttctgtt gcatcaccac agcccactac cgccggaata
     1501 cccagttcac gagcgatgat cgccgcgtga caggtacgac cgccacggtt ggtgacgatg
     1561 gcagatgctt tcttcatgat cggttcccag tccgggtcgg tcatgtcagt gaccagcacg
     1621 tcaccaggtt cgatgcggtt catttcgctg atgtcatgga tgactttcac cggacccgca
     1681 ccgatgcgat gaccgatagc acggccttcg gcgataatct taccctgtga atgcagcgta
     1741 taacgctcca tgacctgacc gcgtgagcgc acggtttccg gacgcgcctg cacaatgaac
     1801 agtttgccgg tgtggccatc tttcgcccac tcaatatcca tcgggcgacc gtagtgtttc
     1861 tcaatttgta cggcctgttt tgccagttcc tgcacttctt cgttggtcag cgagaagatg
     1921 tcacgctgtt cctgcggtac gtcttcgatt ttaacctgct tgccgtgctc ctgggtcggc
     1981 gcgtaaacca tgcggatttt tttcgacccc atggtgcggc gcacgatagc cgggcgattc
     2041 gccgccagtg tcggtttatg cacgtaaaac tcatccgggt taaccgcccc ctgcacgacc
     2101 atctcaccaa ggccccatgc ggaagtgata aacaccacct ggtcaaagcc ggattcggta
     2161 tcaatggaga acatcacgcc agaagatgcg aggtcggagc gcaccatccg ctgaacaccg
     2221 gcggagagcg ccacgccacg gtgatcgtaa ccctggtgca cacgataaga gatggcgcga
     2281 tcgttaaaca gagaagcaaa tacatgtttc actgccacga gaacggcgtc aaacccctga
     2341 acgttgagga aggtttcctg ctgaccggca aaggaagcgt ccggcatatc ttctgcggtg
     2401 gcggaggagc gcaccgcaaa agaggcgttt tcgtcatcgg cggaaagctg agcataggct
     2461 tcgcggatgg cgttttccag ctcaggctgg aagggagtgt cgataatcca ctggcggatt
     2521 tgcgcgcccg ctttcgcaag ctgagtaaca tcgtcaatat ccgttttatc cagcagttca
     2581 taaatgcgct ggtttacgtc gctttggtcc agaaactggt taaacgcgtc ggcggttgtg
     2641 gcgaaaccat tcggaacgga aacacccatt ccggaaaggt tagtaatcat ttcacccagg
     2701 gaggcatttt tgcccccaac cctgtctaca tcattcatgc cgagttggtt gtaccaaagc
     2761 accagcggtg acgagccatt gttggacatc gaacaatcct tttgtgataa atgaacggtt
     2821 tgagaaacac atttctgcgc atttatcttt gcatatttaa ccggatgaaa aaaacgatga
     2881 atcgttcaag caaatatatt tttttacttt ttaagactga tccc
//
LOCUS       DAJTMK010000292         2918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTMK010000292 DAJTMK010000000
VERSION     DAJTMK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.299"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..135
                     /locus_tag="OO135_004108"
     CDS             <1..135
                     /locus_tag="OO135_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5399706.1"
                     /translation="TSLAWGAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF
                     "
     gene            200..1759
                     /locus_tag="OO135_004109"
     CDS             200..1759
                     /locus_tag="OO135_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5399707.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVVRNAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESAR
                     QAAESAASAKQSEEASSSSASAAAQKASESLQSATDAELSKKTAESAAGNAVRDATTA
                     TEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TGPAGATGERGPAGDAGPAGPQGPKGDRGETGLTGNAGPQGAKGDTGAAGPAGAQGPK
                     GDKGDPGVAGPAGPAGAPGPKGDKGDPGVAGPAGPAGPQGPKGDIGAPGQGTELLTTA
                     NTWTQAQTFNGGINGNLTVTGNGSFNDIQIRSDKRNKRNLVKLDNALDRLEALTGYLY
                     EIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGIIALLVEGFKT
                     LRHEIKELREK"
     gene            1845..2357
                     /locus_tag="OO135_004110"
     CDS             1845..2357
                     /locus_tag="OO135_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399708.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            2433..2645
                     /locus_tag="OO135_004111"
     CDS             2433..2645
                     /locus_tag="OO135_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5399709.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
BASE COUNT          783 a          617 c          897 g          621 t
ORIGIN      
        1 acgtctctgg cgtggggggc tggcgtgcag tttaacccga ccgaatccgt ggccattgat
       61 attgcttatg aaggttccgg cagtggcgac tggcgcactg acggtttcat cgtgggtgtc
      121 ggctataaat tctgattagc caggtaacac agtgtcatga cagcccgccg gttcaggcgg
      181 gcttttttgt ggggtgaata tgacagtaaa gatttctggt gtactgaaag acggcacagg
      241 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagta ccacggtggt
      301 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagta tggacgttga
      361 gtatggtcag tacagcgtta ttctgttggt ggagggattc ccgccgtcac atgccgggac
      421 tatcaccgtg tatgaagatt ctcaacccgg tacgctgaat gattttctcg gtgccatgac
      481 tgaggatgat gcccgtccgg aggcactgcg tcgctttgaa ctgatggtgg aagaggtggt
      541 gcgtaacgca gaggaggcga agaagaatgc cggagaggcg gagacgtcag cgaggaatgc
      601 cggcatatca gccagtcagg cagaagagag cgctgcaaat gctgacactt cagcagggga
      661 tgcatcggag tcagcccggc aggcggcaga aagtgcagcc tcagcaaagc agtcagagga
      721 ggcgtcctcg tcctcggcct ctgcggccgc tcaaaaagcc agtgagtcat tacaaagtgc
      781 aacagatgct gagttgtcaa aaaagacggc agaaagtgca gccggtaatg cagtcaggga
      841 tgcaacgacc gcaacagaaa aagcccggga gtcagcagaa agcgcacagt cagcggaaca
      901 aagcaggata gcggcggaag aggccgtaaa ccgaatcccc accgtggtgg gacctcccgg
      961 gccaaagggg gaaccggggc ccgcgggtcc tcaggggccg aagggagata aaggagagcg
     1021 tggcgacacc ggcccggcag gggcaaccgg tgaacggggg ccggcaggtg atgctggtcc
     1081 ggcaggcccg caggggccga aaggcgacag gggagagacc ggtctgacgg gaaatgcagg
     1141 tccacagggg gcaaagggag atacgggagc tgcaggcccg gcaggcgcac aggggccaaa
     1201 aggtgacaaa ggcgatccgg gagtggctgg accagcaggt ccggcaggtg cgccggggcc
     1261 gaaaggcgat aaaggtgatc cgggagtagc aggtccagca ggtccggcgg ggccacaggg
     1321 accgaaggga gacataggag cccccgggca aggaacagaa ctgcttacta ctgccaatac
     1381 atggactcag gcacaaactt ttaatggtgg tattaatggc aatttgacgg tgaccggaaa
     1441 cggctcattt aacgatattc agatccgttc ggataaacgc aacaagcgaa atctggtaaa
     1501 actggataat gcgttagatc gtctggaggc acttactggt tatctttacg agatacagta
     1561 ctctgccgac ggttggcaaa cgtcggttgg tttaattgct caggatgcac aaaaagcatt
     1621 gcctgaactg gtaactgaag acgcagacgt tatatctggt gaaaaacgtc tgcgtcttaa
     1681 ctacaacggc ataattgcat tgttagtcga gggctttaaa acacttcgtc atgagattaa
     1741 agaactccgg gagaagtaaa cgacagctgt tgtagtttct ggtttctact gaatttaaat
     1801 tgtggggtga cactcacccc acgcattcag aagggggaga tgagatgggg gtaacatcgg
     1861 gatgggtagg ctcttcggct aagagcgaaa caggtgagca gtggatggga gccgccggaa
     1921 ccaaactggg actgagtaaa ccttttatga tgagccaaat ggttgggcga actatgggct
     1981 gtaaaattgc aactgagtac tataaatgga aatcctctga caaggttgat aactggggcg
     2041 cagttggcgc tgactggcct ttagaagaaa aaagtaaagg tacaattaca aacgccgcaa
     2101 gctgcggatc agggaggctg gtaggggctg tcgttacact ttctcacttt ttgacgaact
     2161 ctacaccgac agctgctgtt tatttagccg gtggtaaagc aggtaacatc accgtaaacg
     2221 taggtggtgc tacacaaacc atgatttatc agggcgttgt tagtgggttc cagtattact
     2281 ggtcaggttc tgttagttcc gctttcgtgg aggcaatgaa aaagacggga gtaccccagg
     2341 atctaaaaat tagttaaatg gtaaataaat aattttaaaa acttcacgtt ctacataccg
     2401 gagacgccgg atatatagga tattgtttta agttgccaga gaaatttttc cggacggatg
     2461 ctgataataa tgatgtgcca atgacggcag catcatggat ggcactgagt gaagcgacag
     2521 agcaggcgat gtttgcgaag ggagtggaga ttaatacacg gcagttgcag atgaaagccg
     2581 aggttgaggc gctgacagac ctgaaggcga tccgcagtta tgttgtcgga tggcctgctg
     2641 gctgaataaa aaaacgggac cacgaccagt cccggaacca tgagttttag ggtattagtt
     2701 tgttatcata gttagcgtgc taagtatgcc atgtcaggtt gattagtgaa gtgatgttgt
     2761 tcgcattttt gcacggcgga gaatattcag atttttggaa atccatattt ttcccgtgcg
     2821 cgattacatg cttcatttac gatgctattt tcgccggaca ttgcgaattc cctgcatgtg
     2881 gacggttttt gtaaacagag caatatgcgt tttctccg
//
LOCUS       DAJTMK010000293         2891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTMK010000293 DAJTMK010000000
VERSION     DAJTMK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.300"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(317..1126)
                     /locus_tag="OO135_004112"
     CDS             complement(317..1126)
                     /locus_tag="OO135_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCR5399710.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQ
                     NEREFIYNVSGFDNLHQKAKEMLSSAEYEIYLNTDFDLHFLKNEICEALNRHVRVIVF
                     SFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLL
                     INIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNC"
     gene            complement(1532..2653)
                     /gene="ugpC"
                     /locus_tag="OO135_004113"
     CDS             complement(1532..2653)
                     /gene="ugpC"
                     /locus_tag="OO135_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCR5399711.1"
                     /translation="MAAVILRKVEKIYDNGFKPVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMTFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRVAVGRAIVRKSDVFLFDEPL
                     SNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRIMQ
                     VDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAQNIRLYIPENKQSV
                     LKAYIDKPVCFGIRPEHISLSVNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFIAK
                     VNDQSITTVDIGKNLYFNLDTSFCHIFDFYTEENITNYL"
     gene            complement(2736..>2891)
                     /locus_tag="OO135_004114"
     CDS             complement(2736..>2891)
                     /locus_tag="OO135_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HCR5399712.1"
                     /translation="LELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIA
                     AMVAPCK"
BASE COUNT          909 a          539 c          494 g          949 t
ORIGIN      
        1 tatcccacca ataatattgc atcatgtgat catcgggatt attctgtttt ttgataacgc
       61 tatcttcagc agaaacagtg agtggagtta tacgatctct tccatatatt ttatgctccg
      121 taataagatg gggaactaat ttatccagtt ctcctatatg accgttcata taccattcca
      181 tcttattctt taaccaacca gtgggtttta tatcgttaaa aaaatcataa atataccttt
      241 gcataaaaaa gtttagacgt tataaaagac aaatagattg tgatcgccta cagacgatca
      301 caatgaaagg aaaatattaa cagtttttgc tatcactcaa atgtttttca tcctgcacca
      361 tatcattgcc agattcatgc aaagtattta ttaatgcatt ggcatcagga tattgttgct
      421 gatacttggt tagatatata tcactatgga tatgttcggc aattatattg ataagcaagc
      481 gattattcga gtataacccc agtgattcag ctcgttgtga aaaaaccatt actgtcttca
      541 tatcaacaac aagcataaaa cgatgagaag ggtaatattc ttcttcatgg ttaaaacggt
      601 aatatagttc tacgcgtggg tcaggagtaa taagtttatt aaatgaaaaa acaataactc
      661 gaacatgtcg atttagagcc tcacaaattt catttttcaa aaaatgtaaa tcaaaatccg
      721 tgttgaggta aatttcatac tctgcagaag agagcatctc tttagctttc tgatgtaaat
      781 tatcaaaacc agagacatta taaatgaatt cacgttcatt ctgaaacatc atctgtgata
      841 attctttttt caagattttt atgttgtcaa tagtgctttt ttcaatttgc gaaagtatga
      901 gttcaggaga ttttgcttca tattctttgg tttcaccatc gacaagaaag atataaccgt
      961 tcttgtaaag attatcaatt gatgagtata cagacgaacg tgaaagtgaa atttcctttg
     1021 caatcttgta accactgcta cgaccgtttt tcaaaagact gatgtaaacc tgtgcatcgg
     1081 tacgagtgaa accaaaattt atcaatttat ttaccaaatg tatcatagac gcgctcctga
     1141 actgaaaatg aaatcttacc aattttaaca ttattaatta acattttcga aaaattgctg
     1201 agataaaatg tgcatttatc gtcattcatc ctcaagctgg ctacaggtct acagcacata
     1261 agcattgtga actgtgaatc tgaatgccct ccaactatgg tcagaagaca tgctctgacc
     1321 taattagtag actcactgag taagcggtgt tttcaacggt ttattcatat atgctcttct
     1381 ttattgatgg aattttgtaa ctcaattacc tggataagaa tcatttcgtg aagttaatta
     1441 cattaccaat agacagttcg agcagtaaga agccttatgg aatggtaagg gtttaatacc
     1501 ggtattcaac ttggggttat tatcatatca atcacaaata attagttata ttttcttctg
     1561 tgtaaaaatc aaatatatga cagaagctcg tatcaaggtt gaaatacaaa ttttttccaa
     1621 tatcgactgt agtaattgat tgatcgttga cttttgcaat aaactctttg ttgttaacgg
     1681 taaagtaaag atatttttca ctccccatat tttcaataat acttaatgca ccagtgactg
     1741 tattcatttc tttgcaattc acactcaaag agatatgttc tggacgaata ccaaaacaaa
     1801 cgggtttatc tatatacgct ttgagtactg attgtttatt ctcaggtatg tacaaccgaa
     1861 tattttgggc aatgtcaata tagagtttac cgtcttcctc gcgtacaacc gcatctatca
     1921 aattcattga cggggaacca ataaagccag caacgaatct gttagcaggg tggttataca
     1981 gattaactgg tgtgtcgacc tgcattattc gcccctgatt aagcacacaa atacgatcac
     2041 cgagggtcaa tgcttcaatt tgatcgtgtg taacataaat catggttgct ggatgaccct
     2101 catccttgag agactgatgc aactgagcaa ttttgactcg cattgaaaca cgaagtttcg
     2161 catcaaggtt agatagtggc tcatcaaata agaaaacatc tgacttacgg acaattgcac
     2221 ggcctaccgc gacacgctga ccacccgaca tttctttcgg ttttcggtaa agtaaatcgc
     2281 taatttcaag tttttctgca acttcttcga caattttctt aatctcttct tttggccttt
     2341 tttgcatttt taaaccaaaa gtcatattgt caaatacagt cttatgggga tacagcgcat
     2401 agttctggaa caccattgca atgctgcgat ctttaggcgg aagggtatta acaaggcggt
     2461 cgctaatgta aatttcacca cctgatacgt cttcaagacc tgcaatcatg cgtaatgtag
     2521 ttgatttggc acacccagat ggtccaacaa aaaccatgaa ttcaccatcg tttatatcga
     2581 gatcaatgcc gtgaacaggt ttgaatccat tgtcataaat tttttcaact ttgcgtagaa
     2641 ttaccgcggc cataatctgc tctcattaac aagaattttg gaatactgat actccactat
     2701 tcaacgaatg ttgaatagtg gagtgctaaa aataactatt tacatggtgc taccatggcg
     2761 gctattgcct ttttatcagg accaatttga atcgtccaaa catactttcc atcggcagga
     2821 atgacgactt ttgtattttt atccatgcct cctttcttga gtgttttagc ctgatttaat
     2881 ggcaattcaa g
//
LOCUS       DAJTMK010000294         2879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTMK010000294 DAJTMK010000000
VERSION     DAJTMK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.301"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(98..466)
                     /locus_tag="OO135_004115"
     CDS             complement(98..466)
                     /locus_tag="OO135_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399713.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            986..1681
                     /gene="minC"
                     /locus_tag="OO135_004116"
     CDS             986..1681
                     /gene="minC"
                     /locus_tag="OO135_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5399714.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFFK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1705..2517
                     /gene="minD"
                     /locus_tag="OO135_004117"
     CDS             1705..2517
                     /gene="minD"
                     /locus_tag="OO135_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5399715.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2521..2787
                     /gene="minE"
                     /locus_tag="OO135_004118"
     CDS             2521..2787
                     /gene="minE"
                     /locus_tag="OO135_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5399716.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
BASE COUNT          829 a          657 c          678 g          715 t
ORIGIN      
        1 aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag taatttggca
       61 gcattaccgc cgaatagaac attatgtaaa caaggcatca ctcacgacac ataaataaca
      121 tctttgtggt gatttgattg atttttccac tccgcttacc atccacacca aaataacgat
      181 catcacgaca taatttttct ttaacatcgc aaaatacacc gttagcgaat gtaaattcgc
      241 tgggatcgta gccttgtaat gattgtaagt tttcagcggc tttttcgccg agatatttcc
      301 ttgttaactc tggggaaatg ccatctgagt tcacacaaat atacgggtcg caaacaacgc
      361 cgcacgccgg agattgaagt ccgccagcgt catgtccaga cgccattgat gagaacatca
      421 tccccacacc tgacaatccg atataaaaaa tacgcatcat attcatagca catcctgata
      481 atccaaccgt acaacactta tgctcatttc atcatcaatc cgtaatcaaa accgctgacc
      541 tggacgagtt acgatgacct gacagaagta tcagacgtct tctgcggttt ctatgataga
      601 gcaattctgt ggcggatatt aagataacgg gtatctgttc tttaatgatg ctgcaaaaaa
      661 tggaaagtta agaaagcctg gcgtggtgaa aaaatacact tgcctcaata ctagatattt
      721 aaacaggtct ggagatgctg tcacgattag ggtgcggaaa aaggaagcgt aaataagaac
      781 aatcaaaagt acgacggcaa tgggttgatt gacaagggta ttttttaagc tatgaatcag
      841 cgccatttat cacagaatag acttttactc tgaataaatg ggagggtgac ttgcctcaat
      901 ataatccaga ctataacatg ccttatagtc ttcggaacat catcgcgcgc tggcgatgat
      961 taatagctaa ttgagtaagg ccaggatgtc aaacacgcca atcgagctta aaggcagtag
     1021 cttcacttta tctgtggttc atctgcatga ggcagaacct aaggttatcc atcaggcgct
     1081 ggaagacaaa atcgctcagg cccccgcatt ttttaaacat gcccccgttg tactcaacgt
     1141 cagtgcactg gaagacccgg taaactggtc agcgatgcat aaggcggtgt cggcaaccgg
     1201 tttgcgggtt attggcgtaa gtggctgcaa agatgcgcaa cttaaagccg aaattgaaaa
     1261 gatggggctg cctatcctga cggaaggaaa ggaaaaagcg ccgcgtccag ctcccgcacc
     1321 acaggctcca gcgcaaaata caacgccggt cacaaaaacg cgtttaatag ataccccggt
     1381 gcgttccggt cagcgtattt atgctccaca atgtgatctg attgttacaa gccacgttag
     1441 cgctggggcc gaattgattg ccgatgggaa cattcatgtc tatggcatga tgcgcggtcg
     1501 tgcgcttgca ggggcaagtg gtgaccggga aacgcaaata ttttgtacga acctgatggc
     1561 ggaactggtg tccatcgcag gtgaatactg gctgagtgat caaatcccag cagaatttta
     1621 tggcaaagcg gcgcgactgc agttagtcga aaacgctttg accgttcaac cgttaaattg
     1681 atcccttttt aacaaggaat ttctatggca cgcattattg ttgttacttc gggcaaaggg
     1741 ggtgttggta agacaacctc cagcgcggcc atcgccactg gtttggccca gaagggaaag
     1801 aaaactgtcg tgatagattt tgatatcggc ctgcgtaatc tcgacctgat tatgggttgt
     1861 gaacgccggg tcgtttacga tttcgtcaac gtcattcagg gcgatgcaac gctaaatcag
     1921 gcgttaatta aagataagcg tactgaaaat ctctatattc ttccggcatc gcaaacacgc
     1981 gataaagatg ccctcacccg tgaaggggtc gccaaagttc ttgatgatct gaaagcgatg
     2041 gattttgaat ttatcgtttg tgactccccg gcagggattg aaaccggtgc gttaatggca
     2101 ctctattttg cagacgaagc cattattacc accaacccgg aagtctcctc agtacgcgac
     2161 tctgaccgta ttttaggcat tctggcgtcg aaatcacgcc gcgcagaaaa tggcgaagag
     2221 cctattaaag agcacctgct gttaacgcgc tataacccag gccgcgtaag cagaggtgac
     2281 atgctgagca tggaagatgt gctggagatc ctgcgcatca aactcgtcgg cgtgatccca
     2341 gaggatcaat cagtattgcg cgcctctaac cagggtgaac cggtcattct cgacattaac
     2401 gccgatgcgg gtaaagccta cgcagatacc gtagaacgtc tgttgggaga agaacgtcct
     2461 ttccgcttca ttgaagaaga gaagaaaggc ttcctcaaac gcttgttcgg aggataagtt
     2521 atggcattac tcgatttctt tctctcgcgg aagaaaaaca cagccaacat tgcaaaagaa
     2581 cggctgcaga ttattgttgc tgaacgccgt cgcagcgatg cagaaccgca ttatctgccg
     2641 cagttgcgta aagatattct tgaggtcatt tgtaaatacg tacaaattga tcctgagatg
     2701 gtaaccgtac agcttgagca aaaagatggc gatatttcta ttcttgagct gaacgtgacc
     2761 ttaccggaag cagaagagct gaaataagcc cgctgtaaaa acgcatttat cttcaaggca
     2821 gagtcatttc tgccttgagt ttttcatccc tctcatccac gttgtggtaa agcggcgag
//
LOCUS       DAJTMK010000295         2857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTMK010000295 DAJTMK010000000
VERSION     DAJTMK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.302"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            84..950
                     /gene="ydiB"
                     /locus_tag="OO135_004119"
     CDS             84..950
                     /gene="ydiB"
                     /locus_tag="OO135_004119"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5399717.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            982..1740
                     /gene="aroD"
                     /locus_tag="OO135_004120"
     CDS             982..1740
                     /gene="aroD"
                     /locus_tag="OO135_004120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5399718.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASALGQISVNDLRTVLTILHQA"
     gene            1886..>2857
                     /locus_tag="OO135_004121"
     CDS             1886..>2857
                     /locus_tag="OO135_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399719.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLLIISPTGLGNRAERGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGC"
BASE COUNT          787 a          646 c          725 g          699 t
ORIGIN      
        1 cctgagaatg atgtgcggtt tggcgagcgt aaatttagca cccggttaaa cacaattaag
       61 catagaggtt aaaggagtta attatggatg ttaccgcaaa atacgaattg attgggctga
      121 tggcctatcc tatccgccac agtttatcgc ctgaaatgca gaataaagcc ttagaaaaag
      181 cgggattgcc atttacctat atggcctttg aagtggataa cgatagtttt cccggagcaa
      241 ttgaaggatt aaaagctcta aaaatgcgcg gaactggtgt atcaatgccg aacaaacaac
      301 tggcttgtga atatgttgat gaattaacgc cagcagccaa actggtgggt gccatcaaca
      361 ccatcgttaa tgatgatggc tacctgcgcg gctataacac cgacggcacg ggccatattc
      421 gcgccattaa agagagcggt tttgatatca aaggcaaaac aatggtgctg ttaggggccg
      481 gaggtgcctc aacggcgatt ggcgcgcagg gggcaattga aggtttaaaa gaaattaaac
      541 tctttaaccg tcgggatgag ttcttcgata aagccctcgc cttcgcgcag cgggttaacg
      601 aaaacaccga ttgtgtcgtc acggttaccg atctcgccga tcagcaagcc tttgctgaag
      661 ccctagcttc cgccgacatt ttaaccaatg gcacaaaagt gggtatgaaa ccccttgaga
      721 atgaatcatt agttaatgat atcagtctgt tacatccggg acttctggtc actgaatgcg
      781 tgtataaccc gcatatgacg aagttattgc agcaggcgca acaagctggt tgcaaaacga
      841 ttgatggata cggcatgttg ttgtggcaag gggctgaaca gttcacgtta tggactggca
      901 aagatttccc tctggaatat gttaaacagg tcatggggtt cggtgcctga caggctgacc
      961 gcgtgcagaa agggtaaaaa aatgaaaacc gtaactgtaa aagatctcgt cattggtacg
     1021 ggcgcaccta aaatcatcgt ctcgctgatg gcgaaagata tcgcccgcgt gaaatccgaa
     1081 gctctcgcct atcgtgaagc ggactttgat attctggaat ggcgtgtgga ccactttgcc
     1141 gacctctcca atgtggagtc tgtcatggcg gcggcaaaaa ttctccgcga aaccatgcca
     1201 gaaaaaccgc tgctgtttac cttccgcagt gccaaagaag gcggcgagca ggcgatttcc
     1261 accgaggctt atattgctct caatcgtgca gccatcgaca gcggcctggt tgatatgata
     1321 gatctggagt tatttaccgg cgatgatcag gttaaagaaa ccgtcgcctg cgcccacgcg
     1381 catgatgtga aagttgtcat gtccaaccat gacttccata aaacgccgga agccgaagaa
     1441 atcattgccc gtctgcgcaa aatgcagtcc ttcgacgccg atattcctaa gattgcgctg
     1501 atgccgcaaa gtaccagcga tgtgctgacg ttgcttgccg cgaccctgga gatgcaggag
     1561 cagtatgccg atcgtccaat catcacaatg tcaatggcaa aaactggcgt aatttctcgt
     1621 ctggctggtg aagtatttgg ctcggcggca acttttggtg cggtaaaaaa agcctctgcg
     1681 ctagggcaaa tctcggtaaa tgatttacgc acagtattaa ctattttaca tcaggcataa
     1741 gcaataatat ttcggcggga atatcctccc ccgccgaact aaaaaaaata tattcaaccg
     1801 tatttaataa aaatatttcg tgagtctctg tgcgctaaat atccatctgg cgtagggaaa
     1861 atcacacctg aatcaggaat taacaatgaa acctgtaaaa ccacctcgta ttaatggacg
     1921 agtgccggtc ctgtcggcac aggaagcggt gaattatatt cccgacgaag caacactttg
     1981 tgtgttaggc gctggcggcg gtattctgga agccacgacg ttaattactg ctcttgctga
     2041 taaatataaa cagactcaaa cgccacgtaa tttattgatt attagtccaa cagggcttgg
     2101 caatcgcgcc gagcgtggta ttagtccgct ggcgcaagaa ggtctggtga aatgggcatt
     2161 atgtggtcac tggggacaat cgccgcgtat ttctgaactc gcagaacaaa ataaaattat
     2221 tgcttataac tatccacaag gtgtacttac acaaacctta cgcgccgccg cagcccacca
     2281 gcctggtatt attagtgata ttggcatcgg gacatttgtc gatccacgcc agcaaggcgg
     2341 caaactgaat gaagtcacga aagaagacct gattaaactg gtcgagtttg ataacaaaga
     2401 atatctctat tacaaagcga ttgcgccaga tatcgccttc attcgcgcta ccacctgcga
     2461 cagtgaaggc tacgccactt ttgaagatga ggtgatgtat ctcgacgcat tggttattgc
     2521 ccaggcggtg cacaataacg gcggtattgt gatgatgcag gtgcagaaaa tggttaagaa
     2581 agccacgctg catcctaaat ctgtccgtat tccgggttat ctggtggata ttgtggtggt
     2641 cgatccggat caaagccaac tgtatggcgg tgcgccggtt aaccgcttta tttctggtga
     2701 cttcaccctt gatgacagca ccaaacttag cctgccccta aaccaacgta aattagttgc
     2761 gaggcgtgca ttattcgaaa tgcgtaaagg cgcggtgggg aatgtcggcg tcggtattgc
     2821 tgacggtatt ggcctggtcg cccgggaaga aggttgt
//
LOCUS       DAJTMK010000296         2847 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTMK010000296 DAJTMK010000000
VERSION     DAJTMK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.303"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            143..418
                     /locus_tag="OO135_004122"
                     /pseudo
     CDS             143..418
                     /locus_tag="OO135_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            518..2458
                     /locus_tag="OO135_004123"
     CDS             518..2458
                     /locus_tag="OO135_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5399720.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSAQDKPASTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARAEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSNLGLMTA
                     FFEAYLDANYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGMDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     GKQDDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTE
                     LHEARKNIPPKNPVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENEVR
                     QTEDKQHQNSEPEEGTPCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKT
                     EKADDLLPENAGSDQHNDSNNETGEDELNWKKQVLIAAVYGLCANPACIAYAPAIPGI
                     AMMIANKLENFGVTSDERLPDF"
     gene            2436..2732
                     /locus_tag="OO135_004124"
     CDS             2436..2732
                     /locus_tag="OO135_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HCR5399721.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          874 a          657 c          686 g          630 t
ORIGIN      
        1 ttatccggtc gataaagtta ttcaccatga taataacgaa atcccggcag gtctttaaaa
       61 cagttccgta ataaatatcc ggtttcattc ttatatgcca gcaatggcag gggttcgctc
      121 aaccttaatt aaggagaaaa acatgattac cagttatgaa gccactgttg taactactga
      181 tgacattgtt cacgaggtta atctggaagg aaagcgtatt ggctacgtga ttaagacaga
      241 aaataaagaa accccattca ctgtggttga tatcgacggt ccatcaggca acgttaaaac
      301 acttaacgat ggtgtaaaaa aatgtgtctg gtgcacatag gaaagaatct gcccgcagaa
      361 aaaaaaaacc gaatttctgg caactctgat tgcaatgaaa ttaaaaggtg aaatctgaaa
      421 aaaagaaagc ctgcacactg tgcaggcctg agtgaagaac ctgggacatt tattcatcac
      481 tcgcagtaat tttaatctga gttgaggtta aaaaacaatg aacaccgata aacaagttta
      541 cccactgtat tacgaagcaa aaaatgacaa agtaagaaaa cgtctcggta ttaaaggcgg
      601 tttttactgg gctgaagcga aaaaattatc cattgccatc tcccgtggtg ctgttgcgat
      661 tgacgatgct ggatacgatg aagatgactt caaaaaacct gttcgcgtca atttgcccgt
      721 tgttgatgac ctcccaccag aaggcgtgtt tgatacggaa ttctgcaacc gttacgaaaa
      781 aggcggggaa gatggcatca caatggtatt tatcgcgccc tcaccctctg cgcaggacaa
      841 accagccagc actgacaata ccaatgttaa tggcgaagac atgacggaga ttgaggagaa
      901 tatgctactc ccgatttctg gtcaggagct gcccattcgc tggcttgctc aacacggcag
      961 cgaaaaaccg gtaacgcacg tttcacgcga cgaactccag gcattacaca ttgcacgggc
     1021 tgaggaacta ccagctgtta ctgccctggc tgtttcgcat aaaaccagcc tgctcgaccc
     1081 gctggaaatt cgcgatctcc acaaactcgt gcgtgatact gacaaagttt tccctaatcc
     1141 tggcaattca aatctgggac tgatgactgc ttttttcgaa gcatatctgg atgccaacta
     1201 caccgatcgc ggtctgctga caaaagagtg gatgaaagga aatcgtgttt cacgcatcac
     1261 gcgcacggct tccggtgcga atgctggcgg cgggaacctc accgatcgcg gcgaaggttt
     1321 cgtccacgat ctgacgtcgc tggcgcgcga cgtagccact ggcgtactgg cccgttcaat
     1381 ggacgtggat atttataacc ttcatccggc acacgctaaa cgcattgagg aaattatcgc
     1441 tgaaaataaa ccaccctttt ctgttttccg cgacaaattc atcaccatgc ctggcgggat
     1501 ggattattcc cgcgccatcg tggttgcgtc agtgaaagaa gcaccgattg gtatcgaggt
     1561 catccccgcg cacgtcactg aatatctgaa caaggtactg actgaaaccg atcatgccaa
     1621 ccctgatccg gaaatcgtgg atattgcctg cggtcgctcc tctgccccga tgccgcaacg
     1681 tgtaacagaa gaaggaaaac aggatgatga agaaaaaccg caaccatctt gcgcaatggc
     1741 agatgaacag gcaacggctg aaacagtgga accggatgca actgaacatc atcaggacac
     1801 gcagccgctg gatgctcagt cacaggtaaa tgctgttgat gcgaaatatc agaaactgcg
     1861 gacagaactc catgaagccc ggaaaaacat tccgcccaaa aatcctgtcg atgcagacaa
     1921 attactggct gcctctcgcg gagaatttgt tgaagggatt agcgacccga atgattcgaa
     1981 atggattccc ggtcatcata tctcctcaaa tgaagtcaaa aaaacggaaa atgaagtgcg
     2041 acagactgaa gataaacagc accagaacag cgaaccggag gagggaacac cctgcaatca
     2101 gactggcgag gataactgcc ctgactgtgg cgcggtgatg ggagacgcaa cttatcagga
     2161 aacttttgac gaaaaaaaac cagatgaagc acaggaagaa gagccgaaga aaacggaaaa
     2221 agctgatgat ttgttgccgg agaacgctgg cagcgatcag cataatgata gcaataatga
     2281 aactggcgaa gatgaactca actggaaaaa acaggtgttg attgcggcgg tttacggttt
     2341 atgtgcgaat cctgcgtgca tagcctatgc gcctgcaatc cctggcatcg caatgatgat
     2401 tgccaacaag cttgaaaatt ttggagtgac cagtgatgag cgcttacccg atttttgatc
     2461 gtattgaaga aatggcatgg gctcgtcact accagcagat cgttcgtgaa gaaaaagaaa
     2521 cggaactggc ggacgacctg gaaaaaggtc tgcctcagca cctgtttgaa tcgctctgca
     2581 tcgaccattt gcaacgccac ggtgccaata aacaggcaat cagccacgca tttgatgatg
     2641 atgttgagtt tcaggagcgc atggcagaac acatccggta catggctgaa accattgccc
     2701 accaccaggt tgatattgat ttggaggact aaaatctatg gcgctttttc aacgagcaat
     2761 aaacacacag gcctatctaa aagcaggaat tatgaggtag cctgagttta acagacactc
     2821 cttcctgaaa tagaatggca tcagaag
//
LOCUS       DAJTMK010000297         2805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTMK010000297 DAJTMK010000000
VERSION     DAJTMK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.304"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            88..2603
                     /locus_tag="OO135_004125"
                     /pseudo
     CDS             88..2603
                     /locus_tag="OO135_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            2614..>2805
                     /locus_tag="OO135_004126"
     CDS             2614..>2805
                     /locus_tag="OO135_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399722.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSST"
BASE COUNT          798 a          679 c          653 g          675 t
ORIGIN      
        1 ggctgcaatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
       61 aatataaaaa acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc
      121 acaccgaaac agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc
      181 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      241 actacaaaaa gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa
      301 ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa
      361 attacattta ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta
      421 cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc
      481 gtgatcaact cgcttgaaca aatcataccc ggtacagctg ctgaatttga tttcaatcat
      541 cagcgactta atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc
      601 tccccttctc gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt
      661 tctgataacc gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat
      721 ggtgctaatt ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag
      781 gcgtcaagct ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct
      841 cagttgcttc tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact
      901 ggcgtgcaac tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca
      961 acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg
     1021 atctatcaaa gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc
     1081 aacagcggcg atttagaagt cacgattgaa gaaagtgacg gtacacaacg tcgctttatc
     1141 cagccttatt cttcattacc catgatgcag cgacctgggc atctaaaata tagcgcgacc
     1201 gctggacgct atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc
     1261 acggcaatat atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa
     1321 gattattatg cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg
     1381 atggatatca acaggctgac acccaattcg ataaccagca ctcttttcat ggctatcaat
     1441 ggcgtacgca gtacatcaaa gatatcccgg aaaccaacac caatatcgct gttagctact
     1501 atcgctatac caacgatggc tattttagtt ttgatgaagc caatacccgc aattgggact
     1561 ataacagtcg ccaaaaaagt gaaattcaat tcaacatcag ccagacaata tttgatgggg
     1621 taagtctgta tgcctccggt tcgcagcaag actattgggg caataacgag aaaaacagga
     1681 atatctctgt tggggtttcc ggccagcaat ggggaattgg ttacagcctg aattatcaat
     1741 acagccgcta cactgatgaa aataatgacc gtgcactctc tttgaatctc agtattccgt
     1801 tagaacgctg gttaccgcgt agccgggttt cctatcagat gaccagccag aaagatcgcc
     1861 caacccaaca tgaaatgcgt cttgatggct cactgctgga tgatggtcgc ctgagctata
     1921 gcctggaaca aagtctggat gacgataaca accataacag tagcctgaac gccagttacc
     1981 gttcacctta tggaaccttc agtgccggat acagttacgg taatgacagc agccaataca
     2041 attacggcgt taccggcggc gtggttatcc atcctcatgg cgtgacgctc tcgcaatatc
     2101 tgggcaacgc ttttgcgctt atcgatgcta acggggcttc tggcgtgagg atacaaaact
     2161 atccggggat tgctactgat ccctttggct atgcagtgat tccttatctc acgacttatc
     2221 aggaaaaccg tctctcggta gatactacgc agctgcccga taacgtcgat cttgaacaaa
     2281 caacacagtt tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc aacgccaata
     2341 tcggttatcg cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg ccctttggcg
     2401 cacttgccag caacgatgat acggggcaac aaagtatcgt cgatgagggc ggcatactat
     2461 atctctctgg gatatcgagt aaatcacaaa gctggactgt acgctgggga aatcaggcag
     2521 atcaacaatg tcagtttgct tttagtacac cggattcaga accaacaacc tctgtattac
     2581 aaggcacagc gcagtgccat taaggataaa aaaatgaaaa gagcgcctct tataacagga
     2641 cttttgttga tatccacatc ctgcgcttat gcctcctcag gagggtgtgg agccgacagc
     2701 actagcggtg cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa ccagacagat
     2761 aacgtgacag gacgggagtt tacctctgca acgctaagta gcact
//
LOCUS       DAJTMK010000298         2780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTMK010000298 DAJTMK010000000
VERSION     DAJTMK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.305"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..516
                     /locus_tag="OO135_004127"
     CDS             <1..516
                     /locus_tag="OO135_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5399723.1"
                     /translation="SQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKT
                     KYFILNNGSVALAGRVLSIDATENGCVIHISLVNLLSTPISNIGFNATWGGEKPVDAK
                     EFARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLP
                     SAENRQKRSKK"
     gene            560..>2780
                     /locus_tag="OO135_004128"
     CDS             560..>2780
                     /locus_tag="OO135_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5399724.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETVKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATDIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     "
BASE COUNT          843 a          638 c          667 g          632 t
ORIGIN      
        1 agtcaactgg aaactattca aaaacaacaa actggcataa taagcagtac aaagacttta
       61 actcacgtaa ttaaatccgt taaagatcaa caaaacactt ttatttttac cgagtttaac
      121 ccagcaaaaa cgaagtattt catcctgaac aatggttcgg tggctttagc gggtcgagtg
      181 ttatccattg acgccacaga gaatggctgt gttattcata tttcactggt caacttatta
      241 agtacaccta tctcaaatat tggttttaat gcgacatggg gtggtgaaaa acctgtcgat
      301 gccaaagaat ttgcccggtg gcaacaatta cttttcaaca catcaatgaa atccacattg
      361 aaattattac caggtcaatg gcaagacatt aatttgaccc tgaagggtgt atcgcccaat
      421 aacctgggat atctgaagtt agccatcaac atggaaaata ttcagttcga caatcttccc
      481 tctgctgaaa atcggcagaa aagaagcaaa aaataaatgc agttagtacg cttataacaa
      541 ggaatagcga gcatcattaa tgaacaaaat atttaaagtt atctggaacc ctgcgacagg
      601 gaattatact gttaccagcg aaacggtaaa aagccgtggc aagaaatctg ggcgcagtaa
      661 gctgttaatt tctgcgctgg ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa
      721 tgccgggaat gacaacggtc agggtgttga ttacggtagt ggatcagctg gcgacggctg
      781 ggttgctata ggcaaagggg cgaaagcaaa tacttttatg aacaccagtg gttccagtac
      841 tgctgtgggt tatgacgcta tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac
      901 ccatgcgatt ggtggtgcat caatggcctt tggggttagt gcaatatcag aaggcgatag
      961 aagtatagca ctgggtgcct cttcgtattc attgggccaa tactcaatgg ccctcggccg
     1021 ttattcaaaa gcattgggta aattgtctat tgctatgggg gactcttcca aagcggaagg
     1081 agcaaacgcc attgccctgg gaaatgccac taaagctact gatattatga gtattgctct
     1141 tggcgacacc gccaatgcgt caaaagcgta ttcaatggcg ctgggagcaa gtagcgtcgc
     1201 atctgaagaa aacgcaattg ccctggggcg tagcagtgta gctagcggta ctgacagcct
     1261 cgcatttggc agacaatcac ttgccagcgc agcgaacgct attgcgatag gtgctgagac
     1321 cgaagccgct gaaaatgcaa ctgctattgg caataatgcg aaggcaaaag ggactaatag
     1381 catggcaatg gggttcggaa gccttgccga taaagtcaat actatcgcat taggaaatgg
     1441 cagccaggct ctggcagata atgcaatcgc cataggccag ggcaacaaag ctgatggcgt
     1501 ggatgccatc gctctgggta atggtagcca gtcgagaggc ttaaacacca ttgccttagg
     1561 cacagccagt aatgcaactg gtgataagag tcttgcgctt ggtagtaata gcagtgccaa
     1621 cggtattaac tctgtcgcgc tgggcgcaga ttccattgcg gatttagaca ataccgtctc
     1681 tgtcggcaat agttcattaa aacgcaagat cgttaatgtg aaaaatggcg cgatcaagtc
     1741 tgacagttac gatgccatta atggttcaca gctttatgcc attagcgact cggtagcaaa
     1801 aaggcttgga ggaggggctg cagtagatgt tgatgacggt actgttacag caccaaccta
     1861 caatttaaaa aatggtagca aaaataacgt aggggctgcg ctcgctgtac ttgatgaaaa
     1921 caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct gctcatggta ctagtagccc
     1981 aactgccagc gtaatcaccg atgttgcgga tggcacgatt tcagcctcca gtaaggatgc
     2041 ggttaacggt tcccaactga aagctaccaa tgacgatgtc gaagccaaca ccgccaatat
     2101 cgctactaat accagcaaca ttgccacgaa tacggcaaat attgccacca ataccaccaa
     2161 tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga
     2221 aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt
     2281 taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac
     2341 caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc
     2401 cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg gtgaggatgc
     2461 gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag
     2521 caaaatcacc aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg
     2581 ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa
     2641 caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa
     2701 atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat
     2761 caccaatatc ctggacggca
//
LOCUS       DAJTMK010000299         2722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTMK010000299 DAJTMK010000000
VERSION     DAJTMK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.306"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..216)
                     /locus_tag="OO135_004129"
     CDS             complement(<1..216)
                     /locus_tag="OO135_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5399725.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKG"
     gene            complement(216..545)
                     /locus_tag="OO135_004130"
     CDS             complement(216..545)
                     /locus_tag="OO135_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399726.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(542..>2722)
                     /locus_tag="OO135_004131"
     CDS             complement(542..>2722)
                     /locus_tag="OO135_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5399727.1"
                     /translation="TGALVYAWYQGDSTLSAFNKTLVLSGNQSGLTADRMLTLSRAGQ
                     AAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGVEVDKVAEAFGKLTTD
                     PTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENM
                     GTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRR
                     GKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARDRAEREASQLKYTGEA
                     QKAYERLQTPLDKYTARQKELNKALKDGKILQADYNTLMASAKKDYESTLKKPSGVKV
                     SAGERQEDRAHAAMLALETELRTLEKHSGVNEKISQQRRDLWEAENQYVVLKEAATKR
                     QLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQAARFEQQQSAKQAAIS
                     AKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWSAEEQLRGSWMAGMKS
                     GWGEWAESATDSFSQVKNAATQTFDGIVQNMAAMLTGSEQNWRGFTRSVLSMLTEIFL
                     KQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAANFHFATGGFTGTGGKYEPAGIV
                     HRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGGTGSPAQMRRSEGIRFEQNNNVVI
                     QNDGTNGLPGPQMLKAVYDMARKGARDEIQAQMRDGGLFSGGGR"
BASE COUNT          513 a          926 c          641 g          642 t
ORIGIN      
        1 gcctttgcca ttcagttcaa aaccactccc ctgaatggga tacggctgat actgtcgccc
       61 ctgccaggtg accggctcac ctttttcgtt ctgctcatta cagaaaaaat aacgttctcc
      121 accgacctct gtcagatcga tttcccagag caccacgctg gccgactgct ccgcacgggt
      181 gcattcattc agtgtttcct gccggatatc ctgcatcagt tcaccacctg ttcaaactct
      241 gcgctgaact caacacgcag catactgacc cgcgacgacc attttgcgca ggtcaccttt
      301 atctgccgcc actcataagg cggcgtccac agaaaggatt tccagccccc gtgctcagcc
      361 agaaacgact caagcgccgt ggcctcccaa cggggaacag aaagcgtcac gctgtacgtt
      421 ttcaggtcgg cattcagccc ggcaggcgct cgctgggaat agccatcacc aaagcgcact
      481 ttcctgacgg aaggggctga agccacatcc ataccgggtt tcactttcca gcggaaggtc
      541 ttcatcgtcc acctccggag aacagaccac catcgcgcat ctgtgcctga atttcatcac
      601 gggcaccctt gcgggccatg tcatacactg ccttcagcat ctgtggacct ggcagaccat
      661 tcgtaccgtc gttctgaatc accacgttgt tgttctgctc aaatctgata ccctctgaac
      721 gccgcatttg cgccggactt ccggtgccac cgacataacc gccggtggca tagccgcgca
      781 tcagccggta aagatttccc acgccaatcc ggctggtcgc ctccttcgtg aagacaaact
      841 ccccgcgatg aacaatcccc gcaggttcat atttaccccc cgtccccgta aatcccccgg
      901 tcgcgaaatg gaagttcgcc gccgcagcct gaatggctgt accgcctgac gcggatgcgc
      961 cgccaccaac agccccgcca atggcgctgc cgatactccc gactatcccc accatcgcct
     1021 gcttcagaaa aatctctgtc agcatggaca gcacagaacg ggtgaaacca cgccagttct
     1081 gttcgctgcc ggtcagcatc gctgccatat tctgtacaat accgtcaaag gtctgcgtgg
     1141 ccgcgttttt aacctgcgaa aaactgtccg tcgcactttc cgcccactcg ccccagccgg
     1201 acttcatccc ggccatccag cttccacgaa gctgctcctc cgcagaccag gtgttcttca
     1261 gtgcagatgt ggccttcgcc agcgcagccg gattatcacc gtacacctca cgaaggcgct
     1321 gctcttccga ctcccgctgc gcctgacggt cggtgagtcc gcgtgctttt gcgctgattg
     1381 ccgcctgctt cgcgctctgc tgctgttcaa accgcgccgc ctgctgtgcc agctcattca
     1441 gccgtttctg gtgttcaatc ttgtcgccca gctcagccag ctggcgtttg tactccagcg
     1501 tttctttctc atgggccagc agggattttt cctgctcaga taactgccgt ttcgtggcgg
     1561 cctctttcag gaccacatac tgattttccg cttcccataa atcccggcgc tgctggctga
     1621 ttttctcatt cacaccgctg tgtttttcca gcgtcctgag ctcggtttca agcgccagca
     1681 tggctgcatg cgcccggtct tcctggcgct caccggcaga caccttcaca cctgacggct
     1741 ttttcagcgt cgattcataa tccttttttg ccgacgccat cagcgtgttg taatccgcct
     1801 gcaggatttt tccgtctttc agggccttat tcagctcttt ctgacgggcg gtatatttat
     1861 ccagcggcgt ctgcaggcgc tcatacgcct tctgcgcctc tccggtatac ttcagctgtg
     1921 acgcctcacg ctcagcccgg tcccttgccg ccagttcacc ggctttttcc atatccgact
     1981 gcagcgtggc cgctgccaga cccagacggg cattttcccg gtcatcccat gccccctgaa
     2041 ggttcgcacg aaaagaggag gtctttcccc ggcgctggct ccggctctgg taccactgcc
     2101 attttttatc cgcctcatca aatgccttct gcgcactggc gagcatatcc gctgaggatt
     2161 caggacgacc gatatccaga atggcatccc acatcgattt gaatgccttc cctgttttat
     2221 ccgcccaggt ctccagtgtt cccatgtttt ctttcaggcg acgggtctgc tcatcaaagc
     2281 ctttcgtggc gatatcgttc gccgcctgca atgccccggc ctcgtctccg gaacgctgca
     2341 gctgtgcaac atacgcaatc tgctctgccg tcacgttacg gaactggcgc gccatcgcca
     2401 tcagtcccga cgtcgggtca gtggtcagct tcccgaaggc ttcagcgact ttatccacct
     2461 ccacaccgga tgcagacgca aaacgcgcga cactctggtt gatggcatca aactgttcac
     2521 caccacgcac accggcattc accagggctg ccagtgactc tctcgcctgg ttaaacgtca
     2581 gccctgctgc ctgcccggct cttgagagag tcagcatacg atcggcagtc agtccggact
     2641 gattaccgga aagaaccagg gttttattaa acgctgaaag cgtggaatct ccctggtacc
     2701 aggcgtacac cagcgcacct gt
//
LOCUS       DAJTMK010000300         2630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTMK010000300 DAJTMK010000000
VERSION     DAJTMK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.307"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(130..321)
                     /locus_tag="OO135_004132"
     CDS             complement(130..321)
                     /locus_tag="OO135_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5399728.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            389..712
                     /locus_tag="OO135_004133"
     CDS             389..712
                     /locus_tag="OO135_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5399729.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            675..1418
                     /locus_tag="OO135_004134"
                     /pseudo
     CDS             675..1418
                     /locus_tag="OO135_004134"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1390..1950
                     /locus_tag="OO135_004135"
     CDS             1390..1950
                     /locus_tag="OO135_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5399730.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            1957..2190
                     /locus_tag="OO135_004136"
     CDS             1957..2190
                     /locus_tag="OO135_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5399731.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
BASE COUNT          637 a          612 c          855 g          526 t
ORIGIN      
        1 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc
       61 ctgacgggct tgtctgctcc cggcatccgc tcacaggcca gctgtgaccg tctccgggag
      121 ctgcatgtgt cagaggtttt caccgtcatc cccgaagcgt gcgaggcagc tgcggtaaag
      181 ctcgtcagcg tggtcgtgaa gcgcttcgca gatgtcagcc tggtcgtcgg cgtccagttc
      241 attcagtttt tccagcaggg tcagcgtctg gcttctgatg aagcgggcca tgttaagggc
      301 ggttttttcc tgtttagtca cagttctgtt tctccatgtg tggggagtct gtcggaggtt
      361 tttaatgata ccggtgaaac gcgaaaggat gctcacgata cgggtcacgg atgaggaaca
      421 tgcgcggctg ctggcacgtt gtgagggaaa acaacttgcg tcatggatgc gtaaggtctg
      481 cctgggggca ccgccatcga aaacgtcagg tcttccgaca ctggcaccgc cgctgttgcg
      541 ccagttcgct tcggtcggca ataacctgaa tcagatagcc cggaagataa acagcggaca
      601 gtggtcgggg catgaccggg ttcatgtggt ggctgcactg atggccatcg gacgtgaact
      661 gagtgagctg agggatgagg tccgtaaaca gggagagcgg gatgatagtt aagtttcatg
      721 ccaggggaaa aggcggtggc agtggtccgg ttgattacct gctgggccgg gagcgtaacc
      781 gggaaggggc gagagttctg cggggtgctc ccgaagaggt gcgggaactc atcgatgcta
      841 cgccgtttgc gaaaaaatac acctcggggg ttctgtcttt tgcagagcag accctgccac
      901 cgggagagcg tgaaagggtg atggagagct ttgagcgggt actgatgccc ggtcttgaaa
      961 aaaatcagta cagcatcctg tgggtggagc accaggacaa ggggcgtctt gagctgaatt
     1021 ttgtcatccc gaatatggag ctggcgagcg gaaaacggct ccagccctat tacgaccgag
     1081 cggaccgtcc gcgaatcaat gcctggcaga cactggtgaa tcaccattac gggctgcacg
     1141 acccgaacgc accggagaac aggcggacac ttgtcacccc gaataatctg ccgaaagcga
     1201 aacaggaggc cgcagaagcg attacgaggg gactggaggc tctttaccat gccggagcgc
     1261 taaaaacgcg tcaggatgtc actgaggcgc ttacagcggc aggttttgag gttgtgagga
     1321 caaccaggag cagtatcagt atcgccgacc cggagggcgg gcgtaatctc aggctgaaag
     1381 gggcgctgta tgagcaatct tttacaaatg gggacagact tcgagaagaa actgaaagag
     1441 cgtgccgtct ctacagagaa catactgaac agcgagttca ggaagctcga agcgtctgta
     1501 gacagggaac tgaacttaaa cgggcagaaa atcagagacg ctatcagcgc acacacagca
     1561 gcggtgaaag aacaactgga gacgctgaac agtgccgtga acgcgcagtt cagcgccaca
     1621 gagaacgaac tggcccggcg gcaggaagag ctgctctgga agctggtcaa agggcggata
     1681 ctgtacccgt cgctgacggc gctttgcgtc acgggcggga tttttctggc gtcatggggg
     1741 ctgattcagt ggcaggaaag ccggattgcg gcgaacatcc tggctatcag ggaccaggaa
     1801 gagacgctgg cgaaactccg ggaaaaaaca tggggtgtga cgtatcacga gggcaggaac
     1861 gggaaatttc tggtattgcc gtccggggtg aagggggaaa acaactggac ggtggagaag
     1921 aagaacgcag taagactggt cagggagtaa gtgagtatga cagagctgga aatgcactta
     1981 ctgaacgcct tagagcaatt gcagcaggac tatacgcagc ggctgaaaga ctgggagagc
     2041 gccttcacgg atttgcagca tatgttcgag ctgacacgac aggagaacgg gcaactgagg
     2101 gaacagtgcg acgacttgag tcagcaggtg cagagattag tcgagcaggt ggaccacttg
     2161 agtcggttat ttatcacgaa cagcagataa gggatgaaca ggaaaggcag gcacgcctgg
     2221 aacgggagcg tgcgcggtcc tggcatgaag agcaggaacg ggaagcacgg gtatggcgtg
     2281 gcccgtcaat agggatatga cggtgagttg ttccgggagg ttgccagatg gtaccggaac
     2341 gcgttaaaaa cgcatccggg cgcgattctg gcgtgtcgtt tttgttgctg tgttttgcct
     2401 ctttgccaga tcgcgctcat tcctcagcct catcccgcga tgtctgcgta tgtcatcaac
     2461 tgaaaagatg gaaaaaaaac agtgtcatga ggtttacgca ggcaaaacca gcgttattca
     2521 catggctgaa tagctatgca tacggttaaa atttatcagg tgcgatcgcg gcagcttttc
     2581 gggtggtttg ttgctatttt tacctgtcag cagccgtgat cgcgctgaac
//
LOCUS       DAJTMK010000301         2606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTMK010000301 DAJTMK010000000
VERSION     DAJTMK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.308"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..71)
                     /gene="csgC"
                     /locus_tag="OO135_004137"
     CDS             complement(<1..71)
                     /gene="csgC"
                     /locus_tag="OO135_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5399732.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDV"
     gene            complement(130..585)
                     /gene="csgA"
                     /locus_tag="OO135_004138"
     CDS             complement(130..585)
                     /gene="csgA"
                     /locus_tag="OO135_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5399733.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNTDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(626..1081)
                     /gene="csgB"
                     /locus_tag="OO135_004139"
     CDS             complement(626..1081)
                     /gene="csgB"
                     /locus_tag="OO135_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5399734.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSAFN
                     QAAIIGQAGTNNSAQLLQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1835..2485
                     /gene="csgD"
                     /locus_tag="OO135_004140"
     CDS             1835..2485
                     /gene="csgD"
                     /locus_tag="OO135_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5399735.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     HSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNDIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMKDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2490..>2606
                     /locus_tag="OO135_004141"
     CDS             2490..>2606
                     /locus_tag="OO135_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="chaperone-like protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgE"
                     /protein_id="HCR5399736.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSI"
BASE COUNT          743 a          571 c          505 g          787 t
ORIGIN      
        1 acatcccctt gctgggtcgt attaaaggtt atctgactgg aaagtgccgc aaggagtaat
       61 aacgcattca tattcttctc ccgaaaaaaa acagggcttg cgccctgttt ctgtaataca
      121 aatgatgtat tagtactgat gagcggtcgc gttgttacca aagccaacct gagtcacgtt
      181 gacggaggag ttagatgcag tctggtcaac tgcagcaccg ttgccaccac cgaactgttt
      241 aaccgtcatt tcagaatttt tgccgttcca ctgatcaaga gtagcgctat taccgaagcc
      301 acgttgggtc agatcgattg agctgtcatc tgagccctga ccaacatctg caccattacc
      361 gccgccatgc tgggtaatag tcaagtcagt gttacgggca tcagtttgca gagcaagtgc
      421 agagttaccg ccaccgtact ggtaaatgtt cagctcagaa tttgggccgc tattattacc
      481 gccaccaccg tggttaccgc cgccgccgta ctgaggaaca acacctgcca gagcgctacc
      541 ggagaatacg attgctgcaa ttgctgctac ttttaaaagt ttcatgtaaa acccccatcg
      601 gattgattta aaagtcgaat ggaaattaac gttgtgtcac gcgaatagcc atttgcgact
      661 gtctctgcac tacaattgcc gttttttgag taccatactg tgtaatattt gctttattac
      721 cagaaccttt ctggataatc atcgcagtat taccataagc accttgcgaa atactggcat
      781 cgttggcact gcccgcctga tcaatatatg caaggttata atctcctgtc tggtcaatct
      841 ttgcccgatt gctactacct tcttgcgcaa caaccgccaa aagttttgag cctccctgca
      901 gtaactgagc actattattc gtcccagctt gaccaattat ggctgcctga ttaaatgcag
      961 acttactcaa ttcatttacc gcgaagttat attctgaatt agctaaatca taacctgctg
     1021 cggctgcaat cccaggcgca cccagtattg ttaacatcat aaataacaat ttgtttttca
     1081 tgttgtcacc ctggacctgg tcgtacattt aagaaattaa atcatttcaa cttggttgtt
     1141 aacgcaacct gtattttgtt aacgctgcgt tacgatggaa agtatgtctg cggaaatatt
     1201 tttaataact cacccgcgcg ttgtattttt ctttttttgt tcatacaaaa gtattaaaac
     1261 ccacgtaaat acgctgttat ctacgcaaaa aatattttgt tttcttttaa atctccgttt
     1321 tccgctatca aaaatcacca gacagtcatt tttcttgccc gtcgctgatt gctgcgatat
     1381 gtcttgcgca caagccgtga caaacctcgt ctttttggag taaaaatttg tccacggagg
     1441 tgcggagaaa aaacaagaac gttttacatg acgaaaggac tacacagaaa tattttttat
     1501 atgtattatt agtaagttat caccatttgt atgatttttt aaaattgtgc aataaaaacc
     1561 aaatgtacaa cttttctatc atttctaaac ttaataaaac cttaaggtta acattttaat
     1621 ataacaagtt acatttagtt acatgtttaa cacttgattt aagttttgta atggctagat
     1681 tgaaatcaga tgtaatccat tagttttata ttttacccat ttagggctga tttattacta
     1741 cacacagcag tgcaacatct gtcagtactt ctggtgcttc tattttagag gcagctgtca
     1801 ggtgtgcgat caataaaaaa agcggggttt catcatgttt aatgaagtcc atagtattca
     1861 tggtcataca ttattgttga tcactaaacc ttctttgcag gcgacagctc tcttgcagca
     1921 ccttaaacaa tcgctggcaa ttacaggaaa attacataat attcaacatt ctctggacga
     1981 tatctcttca ggctctatta ttcttctgga tatgatggaa gcagataaaa agcttatcca
     2041 ttattggcag gatactttga gcaggaaaaa caacgatatc aaaatattgt tgctaaatac
     2101 gcctgaagat tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta
     2161 ttccatgaag gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gcggcgaatg
     2221 ctactttacg caaaagcttg ccagctactt gattacgcat tcaggtaact atcgttataa
     2281 cagcacggaa tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat
     2341 cggcgcgtct aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac
     2401 gcatctttat aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg
     2461 ggcaaacgat aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg
     2521 cagaatttct gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgc
     2581 taactgacca tactgtttca tctatt
//
LOCUS       DAJTMK010000302         2605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTMK010000302 DAJTMK010000000
VERSION     DAJTMK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.309"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..347)
                     /locus_tag="OO135_004142"
     CDS             complement(<1..347)
                     /locus_tag="OO135_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HCR5399737.1"
                     /translation="MPIPLSKDVQINPGVLAVAGNAVDLNGLLLTSNPLLPVGTVVPF
                     SSPDDVAAYFGALSDEYARAQLYFQGFKNATRTPGQLLFSRFSPAPSAAWLRSGSFKG
                     ATVEQLQKLSGTL"
     gene            complement(328..873)
                     /locus_tag="OO135_004143"
     CDS             complement(328..873)
                     /locus_tag="OO135_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399738.1"
                     /translation="MPSGVTLSVTESALYQALGDYHRGLFSDAGIERTQQNQVPMPQG
                     NFITMTGIDVTGLSTAVVAYSAPAQAGEGSQHITRTTKWRCQLDFYGPHAADNAQVLA
                     TLFRSEFSVRLFRQGGGLISPLYCSEPFNTTFPNGQQQYEPRWTLDIQMQINPVVTTS
                     LMFFDNVITRTTEADNANSTQ"
     gene            complement(858..1223)
                     /locus_tag="OO135_004144"
     CDS             complement(858..1223)
                     /locus_tag="OO135_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399739.1"
                     /translation="MNFHGIVSGAVRRVNPFTDALVYRSRGSTQQADYSRVPEYEDPV
                     SVRGQKQAVTQADLRHLDNLNQQGVFATLYTDGNWCGLNRARQQGGDKFVIGDETWLV
                     IEVLEIWPDWTRVIVCLQV"
     gene            complement(1220..1804)
                     /locus_tag="OO135_004145"
     CDS             complement(1220..1804)
                     /locus_tag="OO135_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399740.1"
                     /translation="MVNKVTGGKQFRQKLKQVAANLSLGKKLKVGFLEGATYPDGTSV
                     AYIAAVQEFGGRAVIPAREQTLHFRYNEKTGETGHRFVKAGKGNFVQDVVIPEHTVTI
                     PPRPFFRKMIEHKSPEWGEKMATLLRANDFDTATALVCMGEHIKGQLQMSVRDWESPS
                     NAASTARQKGFNNPLIETGHMMDSVDYSVDGGKK"
     gene            complement(1798..2253)
                     /locus_tag="OO135_004146"
     CDS             complement(1798..2253)
                     /locus_tag="OO135_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HCR5399741.1"
                     /translation="MGTVTFDCRMFVSLYPEFSTVTPELLTAVFNRATALYLDNTDDS
                     PVTDLKEREQLLFLLVAHLCALRGLGNGRDGQAGLVGRITSASEGSVSVSVDNSGSND
                     ASWWYLQTPWGADYWQATAPYRSMQYHSGGAPSRYPGHYYRGYGRGRRW"
     gene            complement(2259..2588)
                     /locus_tag="OO135_004147"
     CDS             complement(2259..2588)
                     /locus_tag="OO135_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399742.1"
                     /translation="MGCKLPNGLVLEVGQKRVQVAGWRNNAVKIVGGYGLTQVDKVFF
                     DAWLAEHGQQPYVKNGVIFAQDKANSAVSQATEQKAVKSGLEPLPQKNPAPGVNRNDE
                     VMGKPQE"
BASE COUNT          628 a          766 c          626 g          585 t
ORIGIN      
        1 atcagcgtgc cggaaagttt ttgtagctgt tcaacagtcg cgcccttaaa cgaaccgcta
       61 cgtaaccagg ccgccgatgg ggcaggactg aaacgggaaa acaacaattg ccccggcgtc
      121 ctggtggcat ttttgaagcc ctggaaataa agctgagcgc gtgcgtactc atcggataat
      181 gcaccaaaat acgcggccac atcatccgga gaggaaaacg gaaccacagt gccgaccgga
      241 agtaacggat tactggtcaa cagcaggccg ttaagatcga cggcattacc cgccacagcc
      301 agcacaccgg gatttatctg tacatcttta ctgagtggaa ttggcattat cagcctccgt
      361 tgtccgggtg atcacgttgt caaaaaacat cagggaagtt gtgaccacag ggttaatctg
      421 catctgaata tcaagcgtcc agcgcggttc atactgttgc tgaccgttgg ggaatgtggt
      481 gttaaagggc tctgagcaat acagcgggga aatcagccca ccgccctgcc ggaaaagccg
      541 tacggaaaat tcagaccgga aaagcgttgc cagcacctga gcgttatctg ccgcgtgagg
      601 gccgtagaaa tcaagctggc aacgccattt tgtggtacgg gtgatatgct gagagccctc
      661 accagcctgc gctggcgcag aatatgccac taccgcagtg gataatccgg taacatcaat
      721 gcccgtcatg gtgatgaaat tcccctgagg catcgggacc tggttctgct gcgttcgttc
      781 aatcccggca tcagaaaaaa gcccccggtg ataatcaccg agggcctgat aaagagcgct
      841 ttccgtaacg gagagggtca cacctgaagg catacaataa ccctcgtcca gtcaggccag
      901 atttccagta cctcaatcac cagccacgtt tcatcgccaa taacaaattt atcgcctccc
      961 tgttgccggg cacggttaag cccgcaccag ttaccatcgg tatacaatgt ggcgaaaact
     1021 ccctgctggt taagattatc aagatgacgt aaatcagcct gggtgacagc ctgcttttgc
     1081 cccctgacgg aaaccggatc ttcatactca ggtacgcggg aataatctgc ctgctgtgta
     1141 ctcccgcggg aacgataaac cagcgcgtct gtaaagggat ttacccgacg tactgcgccg
     1201 gaaacaatac cgtgaaagtt cattttttgc ccccgtctac agaataatca acgctgtcca
     1261 tcatatgtcc ggtttcaata agcgggttgt taaaaccctt ttgccgggca gtggatgcag
     1321 cgttggacgg actttcccag tcgcgaacag acatctgcaa ctgccctttg atatgttctc
     1381 ccatacacac cagagcggtc gcagtatcaa aatcattcgc ccgtaataag gtcgccattt
     1441 tttcgcccca ttcgggactt ttatgttcga tcatcttacg gaagaacgga cgggggggaa
     1501 tggtgaccgt gtgctcagga ataaccacat cctgaacaaa attcccttta ccggctttga
     1561 caaagcggtg cccggtttct cccgtttttt cgttatagcg aaagtggagc gtctgctcac
     1621 gggcgggtat gaccgctctt ccaccaaact cctgaactgc ggcaatataa gccactgacg
     1681 taccgtcagg gtaagttgct ccctcaagaa aacccacttt gagtttttta cccaatgaaa
     1741 ggttcgcggc aacctgtttc agcttctgcc ggaactgttt gccacctgtg actttattta
     1801 ccatcgacgc cccctcccgt atccccggta ataatgcccc ggatatcgcg aaggtgcccc
     1861 cccggaatga tactgcattg agcggtatgg cgctgtcgcc tgccagtagt cagcacccca
     1921 gggggtctgg agataccacc acgacgcatc attactgccg ctattatcca ccgataccga
     1981 tacagaacct tccgacgcac tggtgatacg ccccaccaga ccagcctgcc cgtctctccc
     2041 gtttcccagc ccccgcaatg cgcacagatg cgcaaccagc aggaacagaa gctgttcccg
     2101 ctccttcagg tcggtaaccg gactgtcgtc cgtgttatcc aggtacaacg cggtcgcccg
     2161 gttaaacacc gccgtcagta gctccggtgt caccgtggaa aactccgggt acagactgac
     2221 aaacatccga cagtcaaacg tcaccgtacc catagcgttt actcctgagg tttacccatc
     2281 acttcatcat tgcggttaac gcccggagcc ggatttttct gcggcagcgg ttcaaggccg
     2341 gatttaacgg ctttctgctc cgtagcctgc gagactgcac tgttcgcctt atcctgcgca
     2401 aaaataacgc catttttcac ataaggttgc tgtccgtgct ccgccagcca agcgtcaaaa
     2461 aacaccttat cgacctgcgt cagaccatag ccccccacaa ttttaaccgc gttattccgc
     2521 cagcctgcca cctgaacccg tttctgaccc acttccagca ccagaccgtt cggcagtttg
     2581 cagcccactg ttaccatttc agcct
//
LOCUS       DAJTMK010000303         2564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTMK010000303 DAJTMK010000000
VERSION     DAJTMK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.310"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..937
                     /locus_tag="OO135_004148"
     CDS             <1..937
                     /locus_tag="OO135_004148"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5399743.1"
                     /translation="DVVDLIAQVDLVTTAVGPVVLERIAPAIAKGLVKRKEQGNESPL
                     NIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRIVPPSASATNDPLE
                     VTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNTGHAITAYLGKLAG
                     HQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQRILGRFENPYLKDD
                     VERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHFRSEDDPQAQELAA
                     LIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            937..1524
                     /gene="mtlR"
                     /locus_tag="OO135_004149"
     CDS             937..1524
                     /gene="mtlR"
                     /locus_tag="OO135_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5399744.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1536..1745)
                     /locus_tag="OO135_004150"
     CDS             complement(1536..1745)
                     /locus_tag="OO135_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399745.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            2005..2391
                     /locus_tag="OO135_004151"
     CDS             2005..2391
                     /locus_tag="OO135_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5399746.1"
                     /translation="MYLYQKPSHERSRKNEIKRLSDRLDAIRHQQADLSLVEAADKYA
                     ELEKEKATLEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGL
                     VVVHPMTALGREMGLQEMTGFSKTAF"
BASE COUNT          683 a          634 c          650 g          597 t
ORIGIN      
        1 tgatgtcgtt gatctgattg ctcaggtcga tttagtcact accgccgttg gcccggttgt
       61 gctggaacgt attgctccgg ctatcgccaa agggctggtg aaacgcaaag aacaaggtaa
      121 tgaatccccg ctgaacatca tcgcctgtga aaacatggta cgcggtacca cgcagctgaa
      181 aggccatgtg atgaacgccc tgccggaaga cgccaaagcg tgggtagaag aacacgttgg
      241 ctttgtcgat tccgccgttg accgcatcgt accgccttcg gcttcggcaa ctaacgatcc
      301 gctggaagtg acggtagaaa ctttcagcga atggattgtc gataaaacgc agttcaaagg
      361 cgcactgccg aacatcccaa gcatggagtt aaccgacaac ctgatggcat ttgtcgaacg
      421 taaactcttc accctgaaca cgggtcatgc tataaccgcg tacctcggaa aactggccgg
      481 tcatcagacc attcgtgacg ctattctcga cgagaaaatc cgcgcggtgg taaaaggtgc
      541 gatggaagaa agtggtgcgg tactgatcaa gcgctacggc tttgacgcag acaaacatgc
      601 ggcgtacatc cagagaatcc tcggtcgttt tgagaacccg tatctgaaag atgatgtaga
      661 gcgcgtaggc cgtcagccgc tgcgtaaact gagtgctggc gaccgtctga tcaagccact
      721 gctcggtacg ctggaataca gccttccgca caagaatctg attcagggga ttgctggtgc
      781 aatgcacttc cgcagtgaag atgatccgca ggctcaggaa ctggcagcac tgatcgctga
      841 caaaggtccg caggcggcgc tggcacagat ttccggtctt gatgccaaca gtgaggttgt
      901 atccgaggcg gtaaccgctt ataaagcaat gcaataatgg tggaccaggc gcaggacacc
      961 ctgcgcccga ataacagatt gtcagatatg caggcaacaa tggaacaaac ccaggccttt
     1021 gaaaaccgtg tgcttgagcg tctgaatgct ggcaaaaccg tacgaagctt tctgatcacc
     1081 gccgtcgagc ttttgaccga ggcggtaaat cttctggtgc ttcaggtatt ccgcaaagac
     1141 gattacgcgg tgaagtatgc tgtagaaccg ttactcgacg gcgacggtcc gctgggcgat
     1201 ctttctgtgc gtttaaaact catttacggg ttgggcgtca ttaaccgcca ggaatacgaa
     1261 gatgcggaac tgctgatggc attgcgtgaa gagctaaatc acgacggcaa cgagtacgcc
     1321 tttaccgacg acgaaatcct tggacccttt ggtgaactgc actgcgtggc ggcgttacca
     1381 ccgccgccac aatttgaacc agcagactcc agtttgtatg caatgcaaat tcagcgctat
     1441 caacaggctg tgcgatcaac aatggtcctt tcactgactg agctgatttc caaaatcagc
     1501 ttaaaaaaag cctttcaaaa gtaagcaacg tctgcttact gcccctctac ctgcttcggc
     1561 cgataaagcc gacgataata ctccagacgt tgtagatata acggcacgct ttcatcgggt
     1621 attccggacg gaatcgcgtt acggggagga agttttttca gatactcccg gaacgcctgg
     1681 cttgatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc acctaatttg
     1741 cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccacca attttagttt
     1801 aggttcccgg taaagatgac attaaggaaa acgtgctgaa tcctcaataa tcatccgctg
     1861 ctgtttatta tcttttttct atagatttct taattaatca acgaattgcg tagtttttga
     1921 gcatgttaat gattacgttt cagaactatt acaatgtcat cagttgtgaa caaagcacct
     1981 ggtcgcgcat actaggggct ataaatttat ctttatcaga agccatcaca tgaaagaagt
     2041 cgaaaaaacg aaatcaaacg tctcagcgat cgcctggacg ccatccgcca ccagcaggca
     2101 gatctgtcgc tggttgaagc cgcagacaaa tatgccgagc tggaaaaaga gaaagccacg
     2161 ctggaagcag agattgctcg cctgcgtgag gttcatagcc aaaaactgag taaagaagca
     2221 cagaagctga tgaagatgcc gtttcagcgc gcaattacca aaaaagagca ggctgatatg
     2281 ggcaagctga agaaaagtgt tcgcggactg gtggttgtgc acccaatgac cgcgctgggc
     2341 cgtgaaatgg gcctgcagga gatgactgga ttctctaaga ctgcgtttta agcgcctcag
     2401 acgactggtt cgatcatatc aacccgcatc tggcttgcct tagctggtca atgcggggac
     2461 tctcctgtct actcgcctaa ttaccaaaca attcactccc tttacgtcga tttaccgctg
     2521 gttgatattc tgaaaatgca tttttaccga aatagccacc tata
//
LOCUS       DAJTMK010000304         2532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTMK010000304 DAJTMK010000000
VERSION     DAJTMK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.311"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(54..692)
                     /locus_tag="OO135_004152"
     CDS             complement(54..692)
                     /locus_tag="OO135_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5399747.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(702..1100)
                     /locus_tag="OO135_004153"
     CDS             complement(702..1100)
                     /locus_tag="OO135_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5399748.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(1118..1777)
                     /locus_tag="OO135_004154"
     CDS             complement(1118..1777)
                     /locus_tag="OO135_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR5399749.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(1828..2526)
                     /locus_tag="OO135_004155"
     CDS             complement(1828..2526)
                     /locus_tag="OO135_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR5399750.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
BASE COUNT          643 a          658 c          560 g          671 t
ORIGIN      
        1 agcggcgatc tggtccagat cccgtcgcac aggaacgttg tgtctcagca tgattagcct
       61 ccccaatgcc cacgggcgct ggaagaacga ccatagccgc cgcgcgaaac ggttgacgct
      121 tttttggtgg taaagccgcc tgaagagtaa gactcttttt tgccagaacc ggagcgccag
      181 gagtaatcac cggaagtgtt gcgccagacg gggcgggaag caaatgagga tccgccgctg
      241 ttatagacga acggctcatc gcgatcttta cgaataaccc ggcttaacaa aaatccagaa
      301 acgaccggga tccaactctg ttcaacattg tcgtagtagc aattttcgta cttagactga
      361 caattctgct gagtcatatt cttcggaaca tcggcataaa atgccgcttt ggcgttgttc
      421 cagccacgcg cgcaaatgtc ggcattatta ccgtcatcaa tacaatcctg cactgtcgaa
      481 taaaacgttc cgtcgccgtc gttatcgaca tcgccactat cgccacaacc tttcaataca
      541 aaaaaagcgg caccgcccat tagcgccaac gttaagtatt tttgtgcgta gcgattgcga
      601 cgcggttcaa aaggattatt cggtctacca atgcgactga tcgctccgtg accaatttta
      661 ctgttatttc ttgatttgcg ttttctggcc atgatcgtcc attaccatgt catacaagct
      721 gcgttaaaaa taccgccagc cagcgcggcg gttcccataa acattcctgc tgcggtattg
      781 tgattaataa ttttttcgct taatgcgggc atataaagtc tgacaccagc aaaaacgagt
      841 aactgaatca ccagtgcaat cccgccccag gcgaaatagt ctggaatact caccgcattg
      901 attgccgcac tggataaggg gatcacgtaa cccaacaatg taccgctgaa tgccagtgac
      961 accgcggtat tattgttttt gattaattgc cattcgttgt gcggtgtaat tttggagtag
     1021 ataaacagaa aaataatcac catagccacg ccaataaaaa aataggcact aaaggcaaga
     1081 agtgaatcca gtatatgcat tgtaaacgtc cttgtaatta accaataata tgaagtgatg
     1141 tcagcgggat atcgacaccc aacgcgcgcg aaaataacca ctctggctcg ccgagatcgt
     1201 taaaagattc ctcaccattt aacagcaaat attcgtaagt atcttcggtt acttgccgct
     1261 ggtagcccat ggtaaaatta tggacctccc atttggcatg gttttgattt tctacttttt
     1321 ccagcatgta gacgggttcg atatttcccg gttcttcgct gttaaaaaat cgctgccagc
     1381 gtttttcctg ccagtttaag gtcattgccc ccatcgtttt ggcgttgatc gcctcgcgcc
     1441 agtgactctc tttactgata ccgtagctct cttcatagac aaagagcttg atatcatcaa
     1501 tgtcatcaat atcttcgccg ccggtggtat tgatttgcag aaattcatcg cctgaagtgt
     1561 agtaacggaa aatctggcta ccgcctccca gatcgatgcg gctgacagcg gctaccgtaa
     1621 attcctcacc cggtagcgca atcagcaatt catcttccag caaacgaaac gctaacgtgt
     1681 cgagcgtaaa gccactattg agatgaagtc ccagcggacc gcgagcgata gcaggcttat
     1741 tatccttgcc aaacagacgc tggaaaaaac cagacatatt ttatccttaa aatagttgcc
     1801 agccttttag cgaggctggc aaaaaaatta ctcgccctgt tggcgttgca gtcttgctaa
     1861 tacatcctgg gctctacttt tattgctacc gccaattccg gcttccgcca gcttttcgtc
     1921 aagatcgcga ccgtctgcga ctttctccaa ctgtgcggca gcatccaggc gagcctgacg
     1981 ttcggcctga cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa cgctggaaga
     2041 tgcgccaacg gtagaggttg ttacagcctg ttgtgcacgc tgcatggctt cagtggcttt
     2101 aacgacttcc atttgctgct caaactgagc gatacgctgc gctgtcgctg taactgcttt
     2161 ttccacgcca tcacgggaga cttccagatt cgacaacact tgctcttcag cggtaatgag
     2221 attctcaagg cgcgcgattt cttcagcaac ttcgttaatc aacgacggat taacgttctt
     2281 gcttaacgct tccagcgcac gggcttccag actggctttg cgctcacgta aatctttcag
     2341 cttatcgtga cttaatttca cccgcgccag cagatcaacg cgagattttc cggctttatc
     2401 gagttcagct ttagcgtcac gaatatgctg ttccagcatg cgcacgccct gggtttcttc
     2461 aatggattct tccgcctggg agataaacga tttccccagc gtaaataaac tttttaaaat
     2521 tcccataaat ac
//
LOCUS       DAJTMK010000305         2499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTMK010000305 DAJTMK010000000
VERSION     DAJTMK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.312"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(81..935)
                     /gene="rclR"
                     /locus_tag="OO135_004156"
     CDS             complement(81..935)
                     /gene="rclR"
                     /locus_tag="OO135_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR5399751.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1161..2486
                     /locus_tag="OO135_004157"
     CDS             1161..2486
                     /locus_tag="OO135_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR5399752.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          705 a          559 c          589 g          646 t
ORIGIN      
        1 aagttggaaa tataaatagc tgaaaggctc acaacacccg gaatgcggat tatctttgct
       61 ccgattcctg gcggttttat tcagggtgca agctgtctga ccctttcccg atattctccc
      121 ggagtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcgct ggcatagcct
      181 actgactcag cgatcaccac aacagggagc gtttcccggg aaaacatctg ggccgctatt
      241 tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc acggaaaagc
      301 tgggcaaaac ttgcccggga catgtgggcg atgctggcca gcgattcgac ggtccaagcg
      361 tgtcctggca tttccagcat ttgctgtatt accgcaccaa gacgtggatg cagaagcaaa
      421 ctgagaatgt ttttctctgt attaacctgt gcaatccact cacgcaccgc gagggtaaag
      481 aatgtagcgc agatctggct acacagtgca tccactcccg gcatcgccga tctggattcc
      541 tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ccacgctgtg gttaaccggt
      601 gctaaaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg ttgcaaccga
      661 agagtaccac agacaataca ggtcgattca ttatcgacat gactcaggcg atgagctgaa
      721 ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctctcc cgtcggcatt
      781 tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgataa ttccccggca
      841 ccatgtggaa gctgccagtc acttcctaac acgcaattct tatcgatcgt tccttgtgga
      901 gcgttaagca tcaacaaacg gctaagggca tccatatcag actcctgagt ggtttttgat
      961 tacaaacacc aacaatctta gtaaataact aatctaatac catcaaatag ttaatcatga
     1021 tttttgtttt gtgattctga ttaagaaaaa gaaaatacca gacgataaga caaaatatca
     1081 agcgttgtga agaaatggta tttgctcttt tgcgtctata gtcatgatgt caaatgaacg
     1141 cgtttcgaca ggaaatcatc atgaataaat atcaggcagt tattattggt tttggcaagg
     1201 ctggaaaaac attagccgtc acgctggcaa aagcaggttg gcgagtggct ctcatcgaac
     1261 aatcaaatgc aatgtatggc gggacctgta ttaatatcgg ctgcatccca actaaaacat
     1321 tggttcatga cgcacagcag cacacagatt ttgtccgtgc catacagcgt aaaaatgaag
     1381 tggttaattt tttacgtaat aagaattttc ataatcttgc ggatatgcct aatatcgacg
     1441 tgatcgacgg ccaggcggag tttatcaata atcatagcct gcgtgttcat cggcctgagg
     1501 gaaatctgga gattcatggc gagaaaattt ttattaatac cggtgcacaa accgtggttc
     1561 cgccaattcc tggaattacc accacgccag gagtatatga cagcaccgga ttacttaatc
     1621 taaaagaatt gcctgggcat ttaggtattt tgggcggcgg atatattggc gttgagttcg
     1681 cctctatgtt cgctaatttt ggcagcaagg taaccatttt agaagcagct tcgctgtttt
     1741 tgcctcggga agatcgggat attgctgata atatcgcgac gattttacgc gatcagggcg
     1801 tcgatattat cctcaatgcc catgtggagc gaatcagtca ccatgaaaat caagtgcaag
     1861 tgcatagcga gcacgcccaa ctggcggtgg atgcactgtt aatagcttcc ggtcgtcaac
     1921 cggctaccgc ttcgttacat ccagaaaatg ccggtatcgc agtaaacgag cgcggggcaa
     1981 ttgtcgttga caagcgatta cataccaccg cagacaatat ttgggcgatg ggagatgtta
     2041 ccggcgggct gcaatttact tacatatcac tggatgatta ccgcattgta cgtgatgagt
     2101 tactgggtga aggcaaacgt agtactgatg atcggaaaaa tgtgccttat tccgtattta
     2161 tgacaccgcc cctgtccagg gttggtatga cagaagaaca agccagagag agtggtgctg
     2221 atattcaggt ggtgacattg cctgtagctg caattccgcg tgccagagtg atgaacgata
     2281 cccgtggggt attaaaagcg attgttgata ataaaaccca acgtatatta ggggcatcac
     2341 tgctgtgtgt tgactcccac gagatgatta atatagtgaa aatggtgatg gatgccgggc
     2401 tgccttatag catattacgc gatcagatat ttactcatcc gtcgatgagc gaatcactca
     2461 atgatctatt ttcattagtc aaataaactc aaaatcaga
//
LOCUS       DAJTMK010000306         2498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTMK010000306 DAJTMK010000000
VERSION     DAJTMK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.313"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1322
                     /locus_tag="OO135_004158"
     CDS             <1..1322
                     /locus_tag="OO135_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5399753.1"
                     /translation="LVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTM
                     GPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLF
                     IFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFI
                     PHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSL
                     AMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSID
                     APLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYK
                     WLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFA
                     CLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANGK"
     gene            complement(1323..2231)
                     /locus_tag="OO135_004159"
     CDS             complement(1323..2231)
                     /locus_tag="OO135_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5399754.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            2331..>2498
                     /locus_tag="OO135_004160"
     CDS             2331..>2498
                     /locus_tag="OO135_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5399755.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDE"
BASE COUNT          616 a          558 c          621 g          703 t
ORIGIN      
        1 ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc
       61 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
      121 ttaaacacac gatggggccc ggactggctt atctcgccgc gtggacctac tgggtggtgc
      181 atattcccta tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga
      241 aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
      301 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
      361 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
      421 ccgcgcctgc gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
      481 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
      541 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
      601 aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga
      661 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt
      721 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
      781 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
      841 aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
      901 cttctggtac gcccgttaat ggctattttc tgaccctggt gctggtggca attctgatta
      961 tgctgccgac tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta
     1021 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc
     1081 gcctggcgca gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga
     1141 ccgtcgggat ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga
     1201 aaatggaagc cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt
     1261 ttgtgctggt aggattagga ctgattttcc cgctgctggc gcgtaaagcg aatggtaaat
     1321 aattattgtg gtcggtgacg ctgactctgg cagatctgac ccgtttttac ataatcgaga
     1381 aatgcgcgca agccgcagga catatactgg cggtttggat aatagatctg gaagcctgga
     1441 cgctctgtgc tccagtctcc cagtacacat tctaaacgac cagtatcaag gtactccttg
     1501 atctgttcat agagcagata cccgatacca gcacccatca aaacggcctc cagttcagca
     1561 tcaacatcat ccagaatgat atttcctgcg acagcaattt ccagctcctt tgcaaattgc
     1621 cagtggaaag gtttgccgct gggataacga aaaacaacac attgatgatt aagcagatca
     1681 tgaggatggc ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat
     1741 ttcactggcg ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga
     1801 acgccagcgt caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg
     1861 actttaatat caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt
     1921 gcagctaccc gggcggcatt gattttgagc gttcctgtcg gtgtcagacg aaaatcattc
     1981 atctcatcta acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta
     2041 gaacccgctt ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga
     2101 cgttgttcca gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg
     2161 cccgctgcac gaaagctttg attacgggcc acggcaaaga aagtggcaaa gtcagacata
     2221 ttcatccgca ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacaggat
     2281 tatgccgcct gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg
     2341 aacgtattta tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg
     2401 atgcgatcca tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc
     2461 ggcgtaattt caacccagtg atgacgccgg aagatgaa
//
LOCUS       DAJTMK010000307         2438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTMK010000307 DAJTMK010000000
VERSION     DAJTMK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.314"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            117..236
                     /locus_tag="OO135_004161"
                     /pseudo
     CDS             117..236
                     /locus_tag="OO135_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            280..1080
                     /locus_tag="OO135_004162"
                     /pseudo
     CDS             280..1080
                     /locus_tag="OO135_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1195..1536
                     /locus_tag="OO135_004163"
                     /pseudo
     CDS             1195..1536
                     /locus_tag="OO135_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1519..2356
                     /locus_tag="OO135_004164"
                     /pseudo
     CDS             1519..2356
                     /locus_tag="OO135_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          602 a          599 c          715 g          522 t
ORIGIN      
        1 cttgacgtca tagccgactg gccgataaac cgggtcggcg aactgctccc ctggcgcgta
       61 gcactgccga ctgaataaca catccccgtc aatacggttc ttgctgcacg cttacggcca
      121 tctcagtaat acgggaatac attgagattt tctacaatcg tcagcgtcgt cactctcgtc
      181 tggggaatat ctccccggca gccttcagga taaaatatta tcagatgact gcttagaaaa
      241 agaacaaatg gtggtgtccg ctattgccag tacacctcag ttctgggcct tgcccgtagc
      301 agttattttt atcaccgggc tgcactgaaa gccggtgata aatacgccac gatacgtaag
      361 atgctgacag atatctttaa cagtaattac cagtgttatg gctatcgtcg cctgcatgcg
      421 atgctcaggc atgagggggg gcggctatca gaaaaggttg tacgcagact tatggtggag
      481 gaacagcttg tcgtcagtcg taaccgtcgt cgttgctaca gctcatattg cggagaaatc
      541 ggaccggctc cggataacct tatcgccaga gattttaagg cggagcaacc taatcagaaa
      601 tagctgacag atatcacgga gttccagctc cctgcaggta aagtctggct gtcaccagtg
      661 gtggactgct tcgatggaaa agttgtgagc tggtctctca gtacacgccc tgatgctgaa
      721 ctggtcaaca ctatgctgga tagcgctgtc gaaacgttaa atgctggcga acgaccggtg
      781 atacacagtg acagaggtgg gcattatcgc tggccaggct ggctggaaag agtgaatgca
      841 gcaggtctta ttcgctccat gtcccgtaaa ggatgttcac ctgataatgc tgcatgcgaa
      901 ggctttttcg gcagactgaa aacggaaatg tattatgggc gtaaatggtc gggcatcacg
      961 ccagaaaagt tcatgcagca agtggatgct tacatcagat ggtataacga gcggcgtata
     1021 aaattatcgc tgggtgccgt cagccccaaa atgtaccgcc aacaatgcgg gctggaatga
     1081 taaagcagtc caggaaatcg tccgcatccc cccttcacca tttggattgt ccgctccacc
     1141 caacatgttg tttccttaag gttcctacac cacaaaggaa actctcatgc tgcgcagtgg
     1201 atggctgacg ttgccgacag gcgggaactt cgccagttca ggcagacacc ggaacagcgc
     1261 ttcacgcagg aacaggagca tctgcagccg ctgctgggta cggacttcga tatccgtcat
     1321 gtctcctggg atggctatat cgaggttggc gggaaccgtt acagtgtgcc ggaaagcctg
     1381 tgtggtcagc tggtatcaat acggatctcg ctggatgatg aactgcggat ctacagtaat
     1441 gagcaacagg tagcatcgca ccgtctctgt tcggctgcat acggctggca gaccgtgccg
     1501 gagcatcacg ctccactctg gcagcaggct tcggagcgca tggccgaacg gctgggggag
     1561 atacagaaac gggtaatcac tgtctgtgac cgggaagcgg atatctggca ttacctgtat
     1621 tataaagtca gccacggaca gcgtggtgcg tgctgcacag aatcgccggc tggaagaggc
     1681 acccggcaag ctcttcgaac tgccggaagt cctggcaacc gcaggaagcc acacgctgaa
     1741 tgtgatgcaa aaaggcgggc gggcagcccg tcaggcccgg atgttcatca gttacagcga
     1801 agtcagcata aaaaatcccg acaacagcgg ccaggcgctc ccgctcacgt atgtctgttg
     1861 ccgggagcag gcagaggacg gtgcctgctg gcatctgctg acgtcaggaa aagcggcgag
     1921 tgccgccgat gcacgacgta ttgtcagcca ttacgagcga cgctggctga cagaggaata
     1981 ccacaaggcg tggaaaagtg gtggtacatg gaatcgctgc gaatgcagac ccgggataac
     2041 cttgagcgca tggtggttat ccaggcgttt atagcggtac gggtgctggg cctgcggcag
     2101 ggaggtgtca gtgaagaaac acaaaatgac agctgtgaga aaatactgac gccgacagag
     2161 tggaaactac agtgggttaa gctggaagga aagccgcttc cggtgcaggc accgaccctg
     2221 aaatgggcct ggggcgatgg tatgacagca aacgcacagg tcgtcccggt tggggcgtca
     2281 tgtgggatgg ctggtcagac ttcaggatat ggttgagggt tacctggtga tgaagtctct
     2341 tgatcaggag ctctgatcaa gagacagcag ctttgggggg tgcccctatt aagttgtcaa
     2401 gcatgttatg acccctgcgg ggtataaaaa gtcccgtc
//
LOCUS       DAJTMK010000308         2427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTMK010000308 DAJTMK010000000
VERSION     DAJTMK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.315"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..684
                     /locus_tag="OO135_004165"
     CDS             <1..684
                     /locus_tag="OO135_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5399756.1"
                     /translation="EESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNEPNEKGEPV
                     TWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGATVVRRR
                     VYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDGALFPG
                     RIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNVGNFGG
                     FLSINKLSQ"
     gene            695..1438
                     /locus_tag="OO135_004166"
     CDS             695..1438
                     /locus_tag="OO135_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399757.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            1336..2016
                     /locus_tag="OO135_004167"
     CDS             1336..2016
                     /locus_tag="OO135_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5399758.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNSAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            2257..>2427
                     /locus_tag="OO135_004168"
     CDS             2257..>2427
                     /locus_tag="OO135_004168"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399759.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKNPSDGHGR"
BASE COUNT          528 a          591 c          794 g          514 t
ORIGIN      
        1 gaagaaagtc tgagcgagtc ggttaaatca gagcagtcac cgcgggtggt actctgggaa
       61 atcgacctga cggtacaggg cggtgagcgg tattttttct gtaatgagcc gaatgaaaaa
      121 ggggagccgg tcacctggca ggggcgtaag tatgaggcat atccgattga cggcagcggc
      181 tttgagatga acggccgggg cagcagtgcc agaccgtcgc tgacggtgtc caatctgttc
      241 ggtctggtca ccgggatggc ggaagacctg cagagtctgg tgggggccac ggtggtccgc
      301 cgccgggtgt atgcccgttt tctggatgcg gtgaatttcg ttgcgggcaa tccggaggcg
      361 gacccggagc aggagctgag tgaccgctgg gtggtggagc agatgtcgca gctgacagcc
      421 atgacggcct cgtttgtgct ggctacaccg accgagacgg acggggcgct gtttcccggt
      481 cgtatcatgc tggcgaacac ctgtatgtgg acctaccgct ctgatgagtg tggttacacg
      541 ggcggggctg tggcggatga gttcgataaa cccaccacgg atatccgtaa ggacagatgc
      601 agcaagtgca tgcgcgggtg tgaactgcgc aggaatgtcg gcaattttgg cggtttcctt
      661 tccattaata aactttcgca gtaaatcccg gtttatgaca cagactgaat cagcgattct
      721 ggcgcatgcc cggcggtgtg cgcctgcgga gtcgtgcggc ttcgtgataa gcacgccgga
      781 gggggagtgg tatatccctt gtgtgaatat ttctgcagag ccggaggcgt attttcgtat
      841 cgcaccggaa gactggctgc gggcagagat gcagggggag attgtggcac tggtccacag
      901 tcatcccggt gagctgccct ggctgagcga ggctgaccgg cggctgcaga taaaaagcgc
      961 actgccctgg tggctggtct gccgggggga aattcataaa ttccgctgtg tgccacatct
     1021 gacaggacgg cgctttgagc acggggtgac ggactgttac acgctgttcc gggatgcata
     1081 ccatctggcg ggaattgata tgccggattt tcatcgcgag gatgactggt ggcgcaacgg
     1141 ccagaacctg tacctggaca atatggcggt caccggcttt taccgggtgc ccctgtcctc
     1201 tgcacagccg ggcgatattc tgctgtgctg ctttggtgct tcggtaccga accatgccac
     1261 catttactgc ggcaacggtg agctgcttca ccatctgcct gaacaactga gtaaacggga
     1321 gaggtattcc gaaaaatggc aacgacgaac gcattctgtc tggcgtcacc gccactggca
     1381 cgcatctgcc ttcacgggga tttgcaacga tttggccgcc gcctcagcct gtatgtgaac
     1441 tcggcagcgg aagccattcg cgccctgtcg atgcagatgc cgggctttcg ccgtcagatg
     1501 aacgaaggct ggtaccagat acgtattgcc ggtgatgaca cggcaccgga ggcggtgtat
     1561 gcccgtcttc acgaacagct gggtgaggga acggtcatcc acattgtgcc gcgactggcc
     1621 ggggccggaa agggtggact gcagattgtg ttgggggcgg cagccatcgt gggctctttc
     1681 ttcactgccg gggcatcaat ggcgttatgg ggttcagccc tggcagccgg tggtttttct
     1741 gccaccacga tgctgttttc acttggagcc agcatgattc tgggcggtgt ggcccagatg
     1801 ctggccccga aggcaaaaac accggattac cgcgcaacgg ataacggcag acagaacacg
     1861 tacttttcct cgctggataa catgattgcc caggggaacc cgatgccggt gccttacggg
     1921 gaaatgctgg ttggctcccg ccgtatatcc caggacatca gcacccgtga tgaaggcggg
     1981 ggcggaaagg tcgtggttat cgggcgacag ggataaaaca taaaaaaatc ccgcagtgat
     2041 cgcggagctg cggggacaga caaatgaaga tcaatgtgaa ggagttgttt ttgttactcg
     2101 ggcaaaaaaa cactaacgca gcgaaattat aagcgccaca gtcagtgtgt gaaaatgtga
     2161 agatattcag aaattttatt ccgtcatgac gcaggcaccc ggtgaggtgc ctgttgtttt
     2221 tgtgagtgaa caattatcac ggtaagaggt gatgtaatgg gcaaaggtgg cggcagggcg
     2281 cacacaccgc gtgaggcgaa agacaatctc aaatccacgc agatgatgag tgtgattgat
     2341 gcgattggtg agggaccgat agaaggcccg gtgaaaggcc tgcagagtat tctggtgaac
     2401 aaaaacccct ctgacggaca cggacgg
//
LOCUS       DAJTMK010000309         2408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTMK010000309 DAJTMK010000000
VERSION     DAJTMK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.316"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..722)
                     /locus_tag="OO135_004169"
                     /pseudo
     CDS             complement(1..722)
                     /locus_tag="OO135_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            complement(724..1002)
                     /locus_tag="OO135_004170"
     CDS             complement(724..1002)
                     /locus_tag="OO135_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399760.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            943..1128
                     /locus_tag="OO135_004171"
     CDS             943..1128
                     /locus_tag="OO135_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399761.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(1170..1325)
                     /gene="hokD"
                     /locus_tag="OO135_004172"
     CDS             complement(1170..1325)
                     /gene="hokD"
                     /locus_tag="OO135_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5399762.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1977..2393)
                     /locus_tag="OO135_004173"
     CDS             complement(1977..2393)
                     /locus_tag="OO135_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399763.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          572 a          629 c          559 g          648 t
ORIGIN      
        1 accaccgtct cttcctgtgg tggattctgt tgctggtggg cgtgaggcag cagcgcaaga
       61 ttttttgtgc gctgcttcag tatgctggta gcggtctgct ctcccggcac gatgtcgctt
      121 tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg taataccgct
      181 tccggtagcg cgtccgccac ctgattgcgg accgcctacc aggataattc agccagagat
      241 aattcacgct cctgcgtacc gcttattgcg tgaccgatga cgtcaatcat ccatgctgac
      301 aggttttgat gagcaagttg ctcgagtgat tcggatgtct ggtcacgcaa ctggttgtcg
      361 cagtgccagc acaacaccat tgcgccggta ccataacggt gaatgacggt ttcgctgtga
      421 tgataatcgc cgtgtggcca ctggcaggat ttaatatggc gcaacagcca gtcagacaat
      481 gcaccagcac caccagcagc acgaatcacc cgtgcgttac tgaaaaacgg cagcaatgtt
      541 ttgtcttcca ctagcggctg gcgaacggca ggaacgaccc cggacggcag attacgcatg
      601 cttttcggtt ccggctccac cagtacccgg gtattgtgga ataccggcat ggattcacgg
      661 cctggcttaa ggaccaccag cccgagttcc gtaaccagaa caggtcgaag taatacccgc
      721 acattacctc cagatccgtt gctggaatgt gcaggacgga cgcggtggcc gttcggaata
      781 agggagcctg acgaagatta tccagtgacg ataatcgaga ctgagggctt tcttaatctc
      841 gtatccgtgt ctgcggtagc gctgaatcag ccattcagcc tgttcttcgg tgcaggggtc
      901 gtgctgatac cagtcagatt tgaatgcatg agaacgccgc ccgtgcctgc tggcaggggc
      961 ggcagagtta tccgaattgt aaaatttggt atcgtgcgcc atctgttttc tctgctggcg
     1021 cagcaggtgc cagttgttca ggctgacgga tggattgtaa accagaacga ccagaaaaaa
     1081 caaaacccgc cgaagcgggt taagtgcggg tgcgttgagg atgcctgaca catcagcggt
     1141 ggcgagggat ttctcccccg ccgggtctct tactcctcag gttcgtaagc tgtgaagaca
     1201 gcgacctccg tctggccggt tcggattcgt acctcgcaga ggtctttcct cgttaccagt
     1261 gccgtcacta tgacggttaa acagatgacg ataagggcga ttaacatcgc cttttgctgc
     1321 ttcatagcct gcttctcctt gcctttcggc acgtaagagg ctaacctaca tgtgttcagc
     1381 atggattgag cctcagatta atgttaagcg tcttgcagga cgcgtaatgt taactggggc
     1441 ttttctctat ctgccttttg gtgttcatgc ctgagacaga tagcctcaag cacccgcagt
     1501 cattctactt aactaagatt tccctgcaaa ccgtttttgt ccggcacagt aaatatccaa
     1561 ctaaaccaat agcgttcgct gtatttaccg ccagtattca atgcacatga ccgccatgaa
     1621 cacccctaaa aaaagggcat ttatatgtcc aaacattaat atcaaaacat caattttttc
     1681 catatacctt gctgtgaaga tgatgggcat acatgatgcg aacaaccaga acgcaacaaa
     1741 caaaaactgc aatgcgtttt tcattattcc ccctacaatc aatgtgcaat aacatttaaa
     1801 cacacctcaa tttggccgga catataaata tctaaaccag aaaaaatcac ttacatagcg
     1861 ttacaaactc tttagtctaa atactcatcg taaaacattc cccatactta tcagtccgtt
     1921 ccgcgccagg tagcttattg ccttatctgg caacctataa tcaggtttcc gctttttcag
     1981 ttggctggtc gtttaaccga catagttaac ccattaatct ggttgccgga tgctggtgga
     2041 ttttcgcgtt ttagttgttc ataaaagtgc acagctttaa ccagttcttc tgatgtaacc
     2101 gggactggtg gggcagtgaa taaggcctga atttcatagt tcggcctgtc gttacaatcc
     2161 tcttttttcg gtacatattt ccagtcacca acccacaact ccccctgaga gtccataaca
     2221 ccttttttca cgtagcgata tcgccacgct atcggctctg cttccagcga tgcccgtgca
     2281 attttgaata actcgccctc tactcgcgcc atccctgaat tggggtggca tttcgtaatc
     2341 gctattttta atttggcttc ttcgattaat tgttcttttg ttaattcagt cattttcatt
     2401 accgccct
//
LOCUS       DAJTMK010000310         2404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTMK010000310 DAJTMK010000000
VERSION     DAJTMK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.317"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..574
                     /locus_tag="OO135_004174"
     CDS             <1..574
                     /locus_tag="OO135_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5399764.1"
                     /translation="SCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTL
                     AILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICH
                     EFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGC
                     SPSDYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(617..1768)
                     /locus_tag="OO135_004175"
     CDS             complement(617..1768)
                     /locus_tag="OO135_004175"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5399765.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERISGGTDEIMIYVAGRQLLKDYQNK"
     gene            complement(1782..>2404)
                     /locus_tag="OO135_004176"
     CDS             complement(1782..>2404)
                     /locus_tag="OO135_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HCR5399766.1"
                     /translation="DDFILTVETGPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGG
                     GLDVCYLSFAEVDQHGNVGVHKFNGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEI
                     ADGKLNIVQEGRVKKFIQELPEITFSGKIALERELDVRYITERAVFTLKEDGLHLIEI
                     APGVDLQKDILDKMDFTPVISPELKLMDERLFIDAAMGFVLPEAAH"
BASE COUNT          611 a          588 c          542 g          663 t
ORIGIN      
        1 ttcttgtccg gtaattgccg cggggcaggg aaaagaagtc ataaaaacct tatttaatga
       61 gctaagtgtg attttgccgc aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt
      121 tgcctatacg ttagccattc tttactacga aaattttaaa aatgcttatc gttcggagca
      181 ggggtatatt aaaaaagatg ttctgataaa agatattctt ttctatctga ataataatta
      241 tcgcgaaaaa atcactttag aacagttatc gaaaaaattt cgtgccagcg tcagttatat
      301 ttgccatgaa tttaccaaag agtatcgtat ttcccctatt aactatgtta ttcaacggcg
      361 tatgacggaa gcaaaatggt cactcactaa tactgaatta tcacaggcag agatctcctg
      421 gcgtgtgggt tatgaaaatg tcgatcactt tgccaaactg tttttgcgcc atgtcggttg
      481 ttcgcccagc gattaccgta gacaatttaa aaactgtttt gcggaacaag aaatcctatc
      541 tgaatttcct caaccggtaa gtcttgttgg ataaatctgg cggcgcagtt tgttaagaag
      601 ctgcgccgcc tgcagattat ttgttctgat agtctttcag gagctgccga cctgctacgt
      661 aaatcataat ttcgtctgta ccgccgctga tacgttcaca acggacatca cgccagaagc
      721 gggagacgcg cgcttcatcg gtatagccca gaccgcccat gatttgaatc gcatcatcaa
      781 tgacttccat tgcggtacgt gcgcaataca gttttgccag cgccgcgctg gtgcgcagtg
      841 actgatgctg atcggcttgc catgccactt tcagcaccat gttgcgcatg ttgtcgatct
      901 taatcgccat cagcgccagt ttttcctgga tcatctggtt atgaccaatg ggcttaccaa
      961 aagcgatacg ttggttggca tagcgggcgg catcttcaaa ggcgcattcg gcaaagccgg
     1021 tgctgcgagc ggcgttgatc aggcgctcca tctcaaagtt gtacatcaca ttgaggaaac
     1081 ccattccttc ttcgcccacc atgtcgctct cttcaacctc cacgttgtcg agatagactt
     1141 cgcaggtgct gagcatatgc cagccgattt tatgcagcgg gttaatctta atgccgggct
     1201 tactggagtc gacccaccac agggtgaagg cttttttggg atctttcggt tgcggatcgc
     1261 gcgccaacac cagcatatac gggtactctt tcgcaccggt aataaaggtt ttctgtccgt
     1321 tgatgtaaac cttgccgttt ttacgcgtgt aagtggtagt ggcactgttg ttgtctgagc
     1381 ctgcgcctgg ctccgtcaac gccagggcat aggcgggatc acccgtttcc agggtgctct
     1441 ctgccgtttt acgtagctgc tctgcagaac cgaaacggcg catactgtga atacattgac
     1501 cgttggtaat caaaaatgcc ggagcaccgc attttgacac ttccatcagc gccagcatct
     1561 gggtgacgta atccgcaggg atgccaccga actcttccgg cacgccaagc atagaaatgc
     1621 cgttatccgc cagcgcccgc ataaactcac gcggatatgt cccgttttga tcgcaggtgc
     1681 ggaaatactc ttccgcaaag ttagtcgtaa tcagttcgcg aatactggcc agcagcagtt
     1741 cttgttcttc agttaaagaa aaatccattg tgatactcct gttaatgagc cgcttcaggc
     1801 aggacaaaac ccatcgccgc atcgataaat aatctttcgt ccatcagttt gagttctggc
     1861 gaaatcactg gggtgaaatc cattttgtcg agaatatctt tttgtaaatc gacgccaggg
     1921 gcgatttcga ttaaatgcag gccgtcttct ttcagcgtga atacagcacg ttcggtgatg
     1981 taacgaacat ccagttcccg ttcgagcgcg atttttccgc taaaagtaat ttccggtagt
     2041 tcctgaataa atttcttcac ccgtccttcc tggacgatat ttaatttgcc atcggcaatt
     2101 tctgttttta aactgcccgc ggttaacgtg ccgcagaaaa taattttctt cgaggtggca
     2161 ctgatatcaa taaatccacc ggtacccatg attttgccat tgaatttatg cacgccgacg
     2221 ttaccgtgct ggtcgacttc agcaaaactc aaataacaaa catccagacc accaccgtga
     2281 taaaaatcaa actgggacgt catatccaga atggcacggg tattcacgtt cgcgccaaag
     2341 gcaatcccct gtgaagtaat tccgccaatc ggacctgttt ctaccgtcag aataaagtca
     2401 tcag
//
LOCUS       DAJTMK010000311         2383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTMK010000311 DAJTMK010000000
VERSION     DAJTMK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.318"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            437..2089
                     /locus_tag="OO135_004177"
     CDS             437..2089
                     /locus_tag="OO135_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5399767.1"
                     /translation="MISLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO135_004178"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO135_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          740 a          447 c          555 g          641 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaactg aagccttgca aacggggatg
      421 cgttcataca ggtacaatga tatcgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaacgc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccggg aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgg
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccgtatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gat
//
LOCUS       DAJTMK010000312         2363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTMK010000312 DAJTMK010000000
VERSION     DAJTMK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.319"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            359..1084
                     /locus_tag="OO135_004179"
     CDS             359..1084
                     /locus_tag="OO135_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005112197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5399768.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQSIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(1310..1633)
                     /locus_tag="OO135_004180"
     CDS             complement(1310..1633)
                     /locus_tag="OO135_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5399769.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1859..2023
                     /locus_tag="OO135_004181"
     CDS             1859..2023
                     /locus_tag="OO135_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5399770.1"
                     /translation="MAGFIKGQSLTLLEKLDALDADEQATMCEKLHELAEELQNSIQT
                     RFEVENSTER"
BASE COUNT          789 a          563 c          494 g          517 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tagctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagta gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaacactta caccgacacg
      301 cccttaaaca aaagaaaaaa aaacaataaa aaacaccgca aaaatggatt gttaacaaat
      361 gaaaatactc aattcgaatc tcataacaaa catatcatca acaataagaa atcaccttct
      421 aacatcatcc aaaaatataa aaacacaccc atcaccaaac atggacgtaa caagtcatcg
      481 tcgtttattc cagaaagacg gaataggatt gagccaaaca ggaaaagcaa cacaacttgt
      541 cattatgtcg catggtggat ggaaagagat ctcccatcca caaacactat tcacgcgtca
      601 aaaaggcgat ggatggactg tagtccccaa aaacctaaga attgattttt atacaaaaga
      661 caacgacttc acaaagggac taagcgttct ttcagaggta aacaaacgcc atactgaagc
      721 tcaaaaaggg ttaacaccat cactaaacat aagcaaagat gacctaaaag ttctagcgaa
      781 cgcgcgcaat atacctcaat caaaattaga agaagaaatg atgtcacaat ccatatatag
      841 aaaagaatac gcaacaagta atgagaagat aaaaaactat gctctatatt accatgaaca
      901 agcccaaaat attatacaaa gacatcaaga tggcttagga aacaaaaata ttgatatagc
      961 atttataact gataaaaatc ataagaagca cctttctgac atatttaaag taataaatga
     1021 atcaggagta aaatatgatg taattcactt tggcgcttgc cgagtaaata gagaggagaa
     1081 ataatcatca tttaccataa tcccttaatt gtacgcacca ctgaaacgcg ctgaacgcga
     1141 ttctgacgca gacaccacaa aaatagatgt tttaccccgg aaaaacagga aggatgcgtc
     1201 aggatcgttt tcagctcgct gcatagctat gcatgaaagc gaatggagat cactttggga
     1261 gcttaccgtg ttcataccgt ctgttttcaa cagttttctc cccgggaagc taatctgcca
     1321 taagcctgga taacagggca cggtgatact ccgtaatagc gatcagcacc tccgcatact
     1381 ccctgtcccc gatacgcttg ccgtcgataa agagtttttt ctgtaacgcc cccagtctca
     1441 taacttcttt cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg
     1501 cccgtaaaaa accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt
     1561 cttcttctgc cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac
     1621 ttcgttttgt cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac
     1681 cagggcaatt gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt
     1741 cagcaagctc gaattaccga caaaggcacg gcagtttttt ggcagttaca gtgttgtctg
     1801 acagaaatgc ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaccg tactcaacat
     1861 ggcgggattc ataaaaggcc agagcctgac gctgctcgaa aaactggacg cactcgatgc
     1921 tgacgagcag gccaccatgt gtgagaagct gcacgaactc gcagaagaac tccagaacag
     1981 catacagaca cgctttgaag tagaaaatag cacagagaga taacgctcag gacaggatgc
     2041 cgaacggcac aacagagcgt aaggagaaaa tgtcacataa ggcgctcttc cgcttcctcg
     2101 ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggtgtcagc tcactcaaag
     2161 gcggtaatgc ggttatccac agaatcaggg gataacgccg gaaagagcat gtaagcaaaa
     2221 agccgaaggc agggacaaag gccgcgcagg tggcgttttt ccataggctc cgcccccctg
     2281 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     2341 gataccaggc gtttccccct gga
//
LOCUS       DAJTMK010000313         2357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTMK010000313 DAJTMK010000000
VERSION     DAJTMK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.320"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(160..234)
                     /locus_tag="OO135_004182"
     tRNA            complement(160..234)
                     /locus_tag="OO135_004182"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Arg,seq:cct)
     gene            complement(310..1242)
                     /locus_tag="OO135_004183"
     CDS             complement(310..1242)
                     /locus_tag="OO135_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5399771.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEMDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKKPA"
     gene            1536..2291
                     /gene="mlaA"
                     /locus_tag="OO135_004184"
     CDS             1536..2291
                     /gene="mlaA"
                     /locus_tag="OO135_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5399772.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          568 a          572 c          582 g          635 t
ORIGIN      
        1 gtaaaaatcc ggtgggtaaa attattttat ccacttttta cccgtcatgg agtgcggctg
       61 tcaacgatct gacgcgaacc atgacgaacc gtgaatatac ggaaggcttg atattcaggg
      121 gattttgcgg actggtacgg atgggagcga actgataaat ggtgtcccct gcaggaatcg
      181 aacctgcaat tagcccttag gaggggctcg ttatatccat ttaactaaga ggacaatgcg
      241 gcatgagtat acccgctaat ggactgcgga gtaagtacac tgccgctcga ttgcttaaac
      301 cctcgccatt tatgctggtt ttttatcatt tttcttaatg ttttccgcac gttctgcttt
      361 ttggcgtgct tctgctttgc gcttgttgct catgtcgtta cggatctgtg catgactcat
      421 taacgcgaag ataaaggtgc cgccgcagat attccccgct aaagtaggta gtgcgaaggg
      481 ccaaatgaaa tcgctccagt gcagcgtgcc gttaaacacc agatagagga tttctacaga
      541 accgacaacg atgtgggtgg tgtcacccag tgcaataagc caggtcatca atataatcac
      601 cacaatcttt gccgcacccg ctgcaggaaa catccaaacc atagtggcga tcagccagcc
      661 ggaaatgatc gcgttggcaa acatctcgct gggggtgttc ttcatcacat ccatgccgat
      721 tttgacaaat gcatcgtgag tttcttcatt gaagataggc atatattcaa atgcccatgc
      781 agcaatacct gtcccgagaa tattacccag cagcacgacg ccccataatc gcataagtaa
      841 gccgacgttg ctcattgtcg gtttttgcat gacgggtagt accgcagtca cggtgttttc
      901 ggtaaataat tgctggcggg ccataatgac gataataaaa ccaaaggtat aaccgagatt
      961 ctccaacaag aagctgcccg gcacgccttc cagttcgaca tgaaatatcc cttttgccag
     1021 taacgaagcg cccatcgaca gacccgccgc aatggctgac cacagtagtg ccattgcgtc
     1081 gcgttccagc tctttttcac catcctggcg gatatgttca tgaattgcca tcgcccggga
     1141 ggggagtcga tcttcatcca tttctatttt tttgccgcgc tctttttctt cgctctcaac
     1201 ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt
     1261 agcacgtata gctaaagcgt agcggctttt ttgctcgcaa ctggcgggag ttactctgaa
     1321 aatgtagaaa aggccgcgtt tgcctttttc tgtttctata gaatcaagta gcctacaggg
     1381 cggcggttac caggctatga tcaaatcagc aaatcagggc gtctggacat cagttgacgt
     1441 gctgttacaa tcgcccacac ctaaacaggc ggatacggta tcgttccgtc atggatggca
     1501 aactgcataa gccataaaaa aacagggaga catttatgaa gcttcgcctg tcggcgcttg
     1561 ctctgggaac tacgcttctg gtggggtgtg cgagttccgg tacagatcag caagggcgtt
     1621 ctgacccgtt agaagggttc aaccgcacca tgtacaactt caacttcaat gtattagacc
     1681 cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta
     1741 acggtttgag caactttacc ggcaaccttg aagaacctgc ggtgatggtt aactacttct
     1801 tgcagggcga cccttatcag gggatggtcc actttacccg ctttttcctg aacaccattt
     1861 tggggatggg cggctttatt gatgttgcag ggatggcgaa cccgaaactg caacggactg
     1921 aacctcaccg cttcggtagt acgcttggtc attatggcgt gggttatggg ccttacgttc
     1981 agttaccgtt ctacggtagc ttcacgctgc gtgatgacgg tggtgatatg gcggatggtc
     2041 tttacccggt tctttcctgg ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag
     2101 ggatcgaaac ccgcgctcag ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc
     2161 cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga tttcatcgct aatggcggcg
     2221 aactcaaacc gcaggaaaat ccgaacgcac aagcgattca ggatgattta aaagatattg
     2281 attctgaata agaaacaaat aaaaaaggtg agtcgcaaaa ctcacctgtt ttgttgttat
     2341 ccctcaatta ccacaag
//
LOCUS       DAJTMK010000314         2337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTMK010000314 DAJTMK010000000
VERSION     DAJTMK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.321"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(121..432)
                     /locus_tag="OO135_004185"
     CDS             complement(121..432)
                     /locus_tag="OO135_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5399773.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            595..691
                     /gene="ffs"
                     /locus_tag="OO135_004186"
     ncRNA           595..691
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO135_004186"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            811..1164
                     /locus_tag="OO135_004187"
     CDS             811..1164
                     /locus_tag="OO135_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5399774.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1206..>2337)
                     /gene="pdeB"
                     /locus_tag="OO135_004188"
     CDS             complement(1206..>2337)
                     /gene="pdeB"
                     /locus_tag="OO135_004188"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5399775.1"
                     /translation="IRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHNVV
                     ITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQKGWHRQ
                     VFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLANGKIV
                     GAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQHI
                     SINLESTVLTPEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYREAG
                     HEIYLDDFGTGYSSLSYLQDLNVDILKIDKSFVDALEYKNVTPHIIEMAKTLKLKMVA
                     EGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT          573 a          574 c          614 g          576 t
ORIGIN      
        1 gacgagaagg ggaagggcct tcccgagagt ggtaaaggag aggccctgga aataccccac
       61 agccccgaaa aagagccagt cagttgctta actgactggc attactccgc ccggagaggg
      121 ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata ccatcacacc
      181 ttctgccagt aatgcctgtc gctgacgctg taaatccggt ccggttagcg aaattgtgcc
      241 gtggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac gctttaacac
      301 accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat caccgtaagt
      361 ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg gaaatgaatc
      421 ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa aaacgcccga
      481 gtgaagtcgc attgcgcaag aaaccagcat ctggcacgcg atgggttgca attagccggg
      541 gcagcagtga taatgcgcct gcgcgttggt tctcaacgct ctcaatgggg gctctgttgg
      601 ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag ccaaggcaga
      661 tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct cccaccgttt
      721 cgtcaaaaaa aaccaacatg gctaaacttt aaccacaact gacgttgcaa gaattgtctg
      781 gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt tgtggttgcc
      841 gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggctgc gccattgttt
      901 aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc ggatggcaaa
      961 gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt ctttagctgg
     1021 attgaatacc cttcaaaaga ggtccgcgac gctgctaatc aaaagatgat gtcggaccca
     1081 cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat ctatggcgga
     1141 ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc cgtcagccca
     1201 aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa tgccttgctg
     1261 tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc ttgtttactg
     1321 gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc gatgatatgc
     1381 ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt cagaatgtcg
     1441 acattcagat cctgtaaata acttaaactt gaatatcccg taccaaaatc atcaagataa
     1501 atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt cggatcggca
     1561 aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata gtgattgatc
     1621 atttcacgca gcaattgcgg gattttttcc ggggtgagca cggtggattc aagattgatc
     1681 gaaatatgct gctgtggatg ctgacgcagc cagtcgccca tatcttcaaa gacgcttctt
     1741 ataatcagta gcgtcaatgg ctcagaaagg cctgtttgct gtgccagcgg aataaaacta
     1801 tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc agcaccgaca
     1861 attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc acgattttcg
     1921 atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg cagcacaaac
     1981 atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac ttgccgatgc
     2041 cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt catctccggg
     2101 aagggcagga tatcgtaaat gattccatta ttttcgatat gctcaccggg tgttttttgt
     2161 agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac gttatgggca
     2221 ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc aataaaggaa
     2281 gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta acggata
//
LOCUS       DAJTMK010000315         2330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTMK010000315 DAJTMK010000000
VERSION     DAJTMK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.322"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            530..673
                     /locus_tag="OO135_004189"
                     /pseudo
     CDS             530..673
                     /locus_tag="OO135_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            820..1088
                     /locus_tag="OO135_004190"
                     /pseudo
     CDS             820..1088
                     /locus_tag="OO135_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1482..>2330
                     /locus_tag="OO135_004191"
     CDS             1482..>2330
                     /locus_tag="OO135_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5399776.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSSTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTEPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          777 a          501 c          394 g          658 t
ORIGIN      
        1 cattctattt caggaaggag tgtccgttaa actcaggcta cctcaactct cgtctgggga
       61 atatctcccc ggcagccttc aggataaaat attatcagat gactgcttag aaaaagaaca
      121 aatggtggtg tccgctattg ccagtacacc tcaagcttct taccatattt gaaataaagc
      181 tcaatagcgc aaattttttg ttcttcagta aacatgtttc atcttctgag agtccaggaa
      241 atcgtccgca tccccattac cgtcttatat cactggcgct gacatgtgct cattgatgaa
      301 gtgaatctga atgacatgtt ttgccatatc aacgccaacc ggtgtatatt tcatttgtgg
      361 agtctccagt ctgaggacgc tttatgcatc ccgtattggg cagtatgatg ccggaaagct
      421 gcgaggctcc acacctgcca gtttcaccct tttaaccatc atactactcc gtgtttaatt
      481 gtgtgggatg cgttcattac attttaacag tgtcaccccg tcggccacga aagataaaca
      541 ggtgaccaga aaagggatct tcatccagca catgctatat cttgaacgta acaacctgac
      601 cctccgcacc cgcatcaagc ggctggcacg taaaacaatc tgcttctcgc gttccgtgga
      661 aatccacgaa aagtcatcgg ctccttcatt gaaaaacaca tattctactg attggaagcg
      721 ccacccgaaa aagtatcgtt tttttacagt aaacttcatt tagccgacta taatgtaaaa
      781 aaataaattt tttaccaaaa tataacataa ggaagtaata tgcttacaca aactatattc
      841 ccttgtcttc ccaaaaaaca agaaaatatc atccctgaag tatcaaatac tatattagta
      901 tcttctactg ttactacaga tggatgtact gtttttaata aacaaaaaca gccatatacg
      961 aattgcaaat acctgctacc aaccgaacga aaaagttgaa attaacagct acagaaatac
     1021 aatggttgac aaagataaat gaagccagaa ttgatgaaaa acaaagccaa cggtattctg
     1081 atttctgaag ttaatttcag actcggttaa gctccatttt caaaaaaaaa tatatcaggg
     1141 tttgttacta ataacttctg aaaacagaaa aaaaaataga gcagtaattc tatttcagag
     1201 aggagtaact ccatctatcc tcgatacgta ctgaacgtta tcgtgatata ttcatcatgc
     1261 tttcttcacc attattcgag tacagggaga gacgcaatga ccttaaatac ctttttatgg
     1321 ctgaattacc attctcagga ataacagcag actccttccc atagacggct tagggggaaa
     1381 gcaatacccg gagaaagggt acaaagcaaa ctgttttaaa attaataagc tatattctat
     1441 ttcactagaa ttcttatcgt tgataagaaa ttttagttat aatgattaaa tcaaccaata
     1501 tacaggcaat cggttctggt attatgcatc aaataaacaa tatatactcg tcaactccat
     1561 ttcctttacc tatggaactg actccatctt gtaatgaatt ttatttaaaa gcctggagtg
     1621 aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat cgccttcaat aggctaaaaa
     1681 tatgtttgca aaatcaagag gcagaattaa atttatctga gttagattta aaaacattac
     1741 cagatttacc gcctcagata acaacactag aaataagaaa aaacctatta acacatctcc
     1801 ctgatttacc accaatgctt aaggtaatac atgctcaatt taatcaactg gaaagcttac
     1861 ctgccttacc cgagacgtta gaagagctta atgcgggtga taacaagata aaagaattac
     1921 catttcttcc tgaaaatcta actcatttac gggttcataa taaccgattg catattctgc
     1981 cactattgcc accggaacta aaattactgg tagtttctgg aaacagatta gacagcattc
     2041 ccccctttcc agataagctt gaagggctgg ctctggctaa taattttata gaacaactac
     2101 cggaattacc ttttagtatg aacagggctg tgctaatgaa taataatctg acaacacttc
     2161 cggaaagtgt cctgagatta gctcagaatg ccttcgtaaa tgttgcaggt aatccactgt
     2221 ctggccatac catgcgtaca ctacaacaaa taaccaccga accagattat tctggtcctc
     2281 gaatattttt ctctatggga aattctgcca caatttccgc tccagaacac
//
LOCUS       DAJTMK010000316         2328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTMK010000316 DAJTMK010000000
VERSION     DAJTMK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.323"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..185)
                     /locus_tag="OO135_004192"
     CDS             complement(<1..185)
                     /locus_tag="OO135_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399777.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLNTPGSRTVA"
     gene            complement(523..1977)
                     /gene="ospC4"
                     /locus_tag="OO135_004193"
     CDS             complement(523..1977)
                     /gene="ospC4"
                     /locus_tag="OO135_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5399778.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSK
                     FNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKAN
                     SGDTMLDNAMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          670 a          433 c          412 g          813 t
ORIGIN      
        1 tcagccactg tgcgggagcc tggtgtattg agcccttttc ttgcagcggt aacccattgt
       61 cctaaagtgc cttcaggaag ggataatcgg gaagcgcctt cactgatcga aagctgattt
      121 tcaggaaccg ttttgacggc ttcggcttta aactccgtag agtaacgttg ggtttttctg
      181 ctcattattg gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag
      241 gctacctcac tcccgtaaag acaggagtca ggttcccgct ccgacataac tgcgtgcaat
      301 ggattttgaa ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta
      361 gcggtattag gcacagctat ttaggaaaga gggtccatct ccatctcatt aaatggacca
      421 cgccagacaa ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga
      481 cttcgataat cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt
      541 gcctaatatt gctccatttt ttaataaaaa atcaatcatt tttggatttt tgcttttcat
      601 tgcattgtca agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac
      661 caatccatta ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa
      721 gtattcaaca tcatacaaat tacaatatct attgttgttc attttctcca tctctgcgac
      781 atcttgtttt gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatt
      841 gcctaaagaa taagcaaggg cctgatgagc cattttttta tcagtcactt tattgttaat
      901 ctcttcataa ttagatgctt taatcgcatc ctccaaagat atgtctctga gttttatttt
      961 tttgaagtta tctgtactta gcattcttgg tatatgatac tcaagctgaa acacaaagtt
     1021 tataattcta tccagactaa caggatcaat atttttgtta taacaaaact ctctgaatcc
     1081 ttgatcttta ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc
     1141 tttagtgtta aatattggaa catcagtttt cccctgatta ccatgcacat acctgctaac
     1201 ttcatccaca acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac
     1261 aggtggaata gcattatcaa agtgatcggt taatgtcata tatccatatg gagagtcatg
     1321 atcaatgata tacgcattgg cgccaaaatc tacagtatga tgtgttttat ttagtgggag
     1381 tttttcttga tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagagatttc
     1441 tttaatatcc tcagagtatg tattcttatg tgtttgtata ttttcagact gaattttatc
     1501 aaaggattta atattaagaa tattatttga cagattagag ttactttgat gtctgaagtt
     1561 aaaattagaa ttaataataa tagaaagtaa gtgacgttcg tctgaagaaa gaggatgaac
     1621 tttttgtatt atctcagaga tatagtgcct ggacacctta tcgagatgct caagtgaccg
     1681 aaatgatgac cgtgctccag atggcgcttc aatagaaaga tttagtgatt tgaaactggt
     1741 cccttgagaa aacattttgc tataactttg cgctgctata gacttcctta aaaagttttt
     1801 cacagtatta gcacagtgat ctaaatgttt ttcacttatg ccagaagaag atgagtttgt
     1861 tacgcgtaat atattttttt gtaatgcttt tgttgcttta ttcggattcg tttttccatc
     1921 aacaagatca atattattct gaacattaat atgatttact gcttcaggta ttttcatccc
     1981 ccccccaatt atgtgcgttt ttatctgtgg ttatttagat aacattatct aactggacac
     2041 tttcttgtat gtattatagc taaatgaaca gattttaata tgttgtatgc ttttcactct
     2101 gaaatcccct gaatttaatc acgtttcctt tactgaaggc aattttaacc gtagtatcaa
     2161 ttattaaccc ttgggcttga tatccctctc ctcttctggg cacgggggat gtcagattac
     2221 ttattacaga aaccaaaaga attgtaagcg tcatctcagt gtcgtctatt tcaatgtctg
     2281 atgattggcg atgatagagt ccttgaaccg ccccgggttt cctggaga
//
LOCUS       DAJTMK010000317         2275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTMK010000317 DAJTMK010000000
VERSION     DAJTMK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.324"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..349)
                     /locus_tag="OO135_004194"
     CDS             complement(<1..349)
                     /locus_tag="OO135_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5399779.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            591..884
                     /locus_tag="OO135_004195"
     CDS             591..884
                     /locus_tag="OO135_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5399780.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            956..1615
                     /locus_tag="OO135_004196"
     CDS             956..1615
                     /locus_tag="OO135_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5399781.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1707..2153
                     /locus_tag="OO135_004197"
     CDS             1707..2153
                     /locus_tag="OO135_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5399782.1"
                     /translation="MNDVPFWQSKTLAEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          592 a          492 c          587 g          604 t
ORIGIN      
        1 cgtttcgggt ttgcagcact cgcagctcat atttgccaga agcagggagt gagtattgtc
       61 cgtgactatc gagttctggg gaatatctgg ataaatcaac agaatcatcg attcctggcc
      121 cgaagaggta agtatcagcg ccttcattag agatactcac atgtactttc tgaccttttt
      181 tggcatagaa ggtatatgta tcgtaatcgt atccctttat ttcgccggag tactgagcgc
      241 tgctatggcc ttttctgaac tcaacattga cattcttacc cgcagcaaag ctcgtggagg
      301 ttagcagtgc aaacagcaat acagccttac tgatgctctt gattttcatt ggtagtctcc
      361 tgttcagaat gagtacgatg aactgttata atataacaat ccctaacggg agaattccag
      421 tggcttttct gcgttcggta aaatcccggc gcggcatttt ttgccggggg gattacttat
      481 aaaggggcat ggactcaaca aaccgcagaa atctacagct ggctggcttg tggcgcgggt
      541 ttcattgatg ttagcttatg ccaaaacctg gcatcttaaa gagtaaaagt atgttttgtg
      601 tgatttatcg aagcagcaag cgtgaccaga cctatttata tgtcgaaaaa aaagacgatt
      661 tttcgcgtgt tcctgaggaa ctgatgaaag gttttggtca gcctcagtta gcgatgattc
      721 tgccgttgga tgggcggaag aaactggtca atgctgatat tgaaaaggtt aaactggcat
      781 taaccgagca aggttactat ttgcagttac cgccaccacc cgaagatttg ctgaagcaac
      841 atctttccgt catggggcag aaaacagacg acacgaacaa ataaccgata tccggcggtg
      901 gcattatctt tgtcggcgcg ggttttcata tccacgataa ggagagggga acgttatgta
      961 tcaacatcac aactggcaag gtgcgctgct ggattatccg gtgagtaaag tagtctgtgt
     1021 tggcagtaac tatgccaaac atattaaaga gatgggcagc gcagtgcccg aagagccagt
     1081 gctgtttatt aaaccagaaa cggcactgtg cgatctgcgg cagccattgg cgatcccatc
     1141 cgatttcggt tcagttcatc atgaagtcga actggcggtg ttgattggtg cgacgctgcg
     1201 tcaggcaacg gaagagcatg tccgcaaagc cattgccggt tacggcgtgg cgcttgatct
     1261 gacattgcgt gatgttcagg gaaaaatgaa gaaagccggg cagccgtggg aaaaggctaa
     1321 agcgtttgat aactcttgtc cgctttccgg gtttatcccc gcggcggaat ttaccggcga
     1381 tccacaaaat acaacgctgg gcctgagcgt aaacggcgaa caacgccagc aaggtacgac
     1441 tgcggacatg atccataaaa tcgttccgtt gatcgcttat atgagcaagt tttttaccct
     1501 caaggccggt gacgttgtgt tgacaggcac gcctgatggc gtcggcccgt tgcaaagcgg
     1561 tgatgagctg acagtcactt tcgatgggca ttctttgaca actcgcgttt tgtaatactt
     1621 tttgccgcct gaaagcggcg gcaaaacttg catcgctgtg ccagactggt tataaggtgc
     1681 gttttaacgt aatggcggaa cacctgatga acgatgtacc tttctggcaa agtaaaaccc
     1741 tggccgaaat gagcgatgcg gaatgggagt cgttgtgtga tggttgcggt cagtgttgcc
     1801 tgcataaact gatggatgaa gacaccgacg aaatctactt cactaacgtc gcctgtcgcc
     1861 agctcaatat taaaacctgt caatgtcgga actacgaacg tcgtttcgag tttgaacccg
     1921 actgcattaa attaacccgt gaaaatctgc caacattcga atggctgcca atgacctgcg
     1981 cttatcgttt gctggcggaa ggtaaagatt tacctgtgtg gcatccgcta cttactggtt
     2041 cgaaagcggc aatgcatggt gaacgtatct ctgtgcgtca tatcgcagtg aaagaatcag
     2101 aagtgattga ctggcaggat catatcttaa ataaacctga ctgggcgcag tgataaattt
     2161 aatagatgtt gatgggggct ttcttaatat acgtctggat gctcgccatt tcgtagccct
     2221 gtttttgaaa gcgctctgtc ataatgagag ttacccgata ccttattatc aacag
//
LOCUS       DAJTMK010000318         2263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTMK010000318 DAJTMK010000000
VERSION     DAJTMK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.325"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..108
                     /locus_tag="OO135_004198"
     CDS             <1..108
                     /locus_tag="OO135_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5399783.1"
                     /translation="NKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(135..1034)
                     /locus_tag="OO135_004199"
     CDS             complement(135..1034)
                     /locus_tag="OO135_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5399784.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(1145..>2263)
                     /locus_tag="OO135_004200"
     CDS             complement(1145..>2263)
                     /locus_tag="OO135_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5399785.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWII
                     AGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT          690 a          547 c          525 g          501 t
ORIGIN      
        1 aataagaatg cagaaaacgt gaaggaattt ctgcaatctt atcaatcacc cgaagtcgct
       61 aaagcggcag aaaccatttt taacggtggc gcggtgcctg gctggtaatt ctcactcatg
      121 ccgcgccgga cgatttattt ttctggccgg cgacgggaat ccattgaaat caaaatcacc
      181 gatgacagaa tcagcagcgt tcccagccag tccggtaatg tgaacgtgat ccccagcaac
      241 agcaaagaga gtagcgcgct acttaatggt tctgcacagc tcaaaatgct ggcttttgga
      301 ccgccaatta attgtgctcc tttcaggtac agactaaatg tcagggacgt accaatgacc
      361 accaaataaa aaaacgccag aatcaaactg ccgttaacga caaagtttgt tccttgtctg
      421 gcataaaaag gcaacagaat cagaccgcca atcagcatac tccagccgac gactggtaac
      481 gtgccatagc gggcaattag cgttgaggga taggtggtat agaatgcagc agcaaatgcc
      541 gaggcaatgc cccagaacaa cgcggcagga gagagcgata atgacgtcgg attaccgtgt
      601 gtcaccaata aaaaagtccc gaccagcgat gtcaaaatag cgcagaaaac cagaatgccc
      661 gggcgcgatt tacgcaccag tgagaaccag gcgacgataa tcgtcggtga gaggaattgc
      721 agcaccgttg ccgtggctgc gttcgatttt tcgatggtta gcaaaaaagt gagctgtaca
      781 gttagcgcgc caaccacgga aaaaatcagc aggctaatgg catctttatg attgttaata
      841 atagaaaaga ttttatcgcc atgaacaaat gacagcgtca gtagaatcaa accggcgaat
      901 atcaaacgcg tcatagtcaa aaactgcgac gacatctggc tttgctccat gatgtattgc
      961 gcgcagaccc ctgaacttcc ccacaacacg gcggcaatca gaacgttcag catccccttt
     1021 ctggtggaac ccatttttcc ctctgcatac tgttttgtcg tttatgtatg caggcatgat
     1081 agcaaaaatg gcgaggatgg ctctgtttgc gccacgccga tttttcttag cccagactgg
     1141 tgagctaaat atctttaata aacagcaggc aaatcatcgc aatacccaac atccctatcg
     1201 ccagccagaa caacgcatgg tagctccaga tttcaaccat aattccgtca acagagccgg
     1261 cgataatcca gccgacgcgt gaagtatttg catataacgt tgtcgcagag cctatttttt
     1321 caggcatcaa gtcctgaaaa taaagcatgc cgataccaca gagaatacca aggaagatgg
     1381 catttagaat ttgcaattcc agctcaaccg ccggagtcgt cgccatgagt acgctggcgt
     1441 aaaaacacat accactcacg atagcaatga gcattaatag tcgcttgcca atacgtttca
     1501 tgtaatagcc tgcgattaac atcatcggaa tttccagacc ggcagcgata ccaatcattt
     1561 ctccagccag tttatcggtt agatgcagtt catcaataat aaatagcggc atatttatca
     1621 tgtagagatt attcgccgcc cacatcattg aacagaccac aaaaagtagc cgcgtatccc
     1681 gcttcctatg ggtggagggt aaaatttcta cgggttgggt aacgacagga atatttcttt
     1741 gtattgatgg caaaaacaac cagactatca gcccgcaaac aacaaatgcg atggcagcgg
     1801 tgagatacat cactttaaaa ctaaacccca ttgccagttc ataagcgagc ggtggcccga
     1861 taacccaggc aagcgagatc tgagcacgta aaaatgtact gaacatgacc gtctcacggc
     1921 ctgttctgtc ggcgtgttca cgggcgaggg cgaacatttg cgggtttgcg gtagaagcaa
     1981 aactactcag aagtacgccc gtagagagga gaatgaagta gttgcgattc cacgcaaaaa
     2041 gcgtgcaggc cagcactcca aataagcaac atagcagaat cagtaattta cggtcgcctt
     2101 gtttatcgga gtgccttgcc agaaattgac tgaccagaat tcccataata gcgctaccgg
     2161 tgaagaaaaa acctaccatt ataggacggg ctttcagttc atctgcgagg aatatactta
     2221 gggtaggagt ctgaagagcg cccgcaatac ccgtcagaaa ggc
//
LOCUS       DAJTMK010000319         2225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTMK010000319 DAJTMK010000000
VERSION     DAJTMK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.326"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="OO135_004201"
     CDS             complement(135..1589)
                     /gene="ospC2"
                     /locus_tag="OO135_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5399786.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYILSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKGKDSKTIDFLLKNGAVSGKRFGR"
     gene            2014..>2225
                     /locus_tag="OO135_004202"
     CDS             2014..>2225
                     /locus_tag="OO135_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5399787.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNR"
BASE COUNT          684 a          412 c          373 g          756 t
ORIGIN      
        1 tctccaggat tcccggggcg ttcaccacat gcaatgtgtt gttgcagggg aacggtctgc
       61 accatcccct atatcgatca atataacaaa aatgacaacg tcagattcag ggcacaacct
      121 aaatatattt taggtcacct cccaaatcgt ttgcctgata ccgctccatt ttttaataaa
      181 aaatcaattg tttttgaatc tttgcctttc attgcattgt caagcatagt gtccccacta
      241 tttgcttttt gaaatctttt gtttacatca accaatccat tattaataaa atattctagc
      301 actttatagt tggcactatc ttcactaagt aaatactcaa catcatacag ctcacaatac
      361 atattgttgt tcattttctc catctctgcg acatcttgtt ttgtaaaatt aaatttagat
      421 agtatataaa gtgccatatc agattttgca ttgcctagag aataagcaag agcttgatga
      481 gccatttttt tatcagtcac cttattgtta atttcttcat aattggatgc tttaatggca
      541 tcctccaaag atatatctct gagtcttatc tttttgaagt tatctgtgct tagcattctt
      601 ggtatatgat actcaagctg aaacacaaag tttataattc tatccagact aacagggtca
      661 atgtttttat tataacaaaa ctctcggaat ctttgatctt tactctttct tataaaatca
      721 ataagatgca acccaattcc aagcctcata tctttagtat taaatattgg cacatcagtt
      781 tttccctgat taccatgcac atacctgcta acttcatcca caacctcttt aaagttatct
      841 aagaaaaatg attggtgctc atggtaaaaa acaggtggaa tagcattatc aaagtggtcg
      901 gttaatgtca tatatccata tggagagtca tgatcaatga tatacgcatt agcaccaaaa
      961 tccacagtat gatgtgtttt atttagtggg agtgtttctt gatgattgga aatttcaacc
     1021 ccaaaaaaaa caaaatcgtg gttagctatt tcttcaatat cctcagagaa tgtgttttta
     1081 tatgtttgta tattctcaga cttaatttta tcaaaggact taatattaag agtattattt
     1141 gacaggttag cgttactttg atgtctgaag ttaaaatcag aattaataat aatagagagc
     1201 aagtgacgtt catccgaaga aagaggatga gttttttgta ttatttcaga aagatagtgt
     1261 ctggacacct tatcgaggtg ctcaagtgac cgaaatgatg accgtgctcc agatggcgct
     1321 tcaatagaaa gatttaatga tttgaaactg gtcccttgag agaacatttt actataactt
     1381 tgcgctgcta tagacttcct taaaaagctt ttcacagtat cagcacagtg atctaaatgt
     1441 ttttcactta tgccagaaga ggatgagttt gttacgcatg atatgttttt ttgtaatgct
     1501 tttgtatctt tgttcggatt tatttttcca tcaacaagat caatattgtt ctgaacatta
     1561 atatgattta ctgcttcagg tattttcata aaacatcccc cattatgtgc gtttttatct
     1621 gcagttattt agaataatat tatctaactt aatattttct tgtatgtatt ataacgaaca
     1681 tattttaata tgttgtacga ttttcactct gaaatcccct gagtttaatc acacttcctt
     1741 tactgaaagg aattattgca acagtattat ttgtttttgg atatatatct tgcgccttgt
     1801 tatgtagtta atgctcagaa tatgatagat aaaatagaga cgcagaacag ggcatgctca
     1861 taacatgatg ataacataaa aaattagtaa caataaatga aagaatcatg ggcgctcaac
     1921 aaaaagagag ctaaatagct gcacggaata gtaggctgtg tcccttaatc atctgagtta
     1981 tagtaactgt ctgtttacac aacatattga actatggctc gctacgacct tcccgatgag
     2041 gcatggacca tcatcaagcc cctgttacct cctgaacccg ccacaccacg ggccggacgc
     2101 ccatgggctg agcaccgtaa aatcatcaat ggcatgttct gggtgttatg ttccggtgca
     2161 ccatggcgcg atttacccga acgatatggg gcatggaaaa ctgtttataa ccgctttaac
     2221 cgatg
//
LOCUS       DAJTMK010000320         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTMK010000320 DAJTMK010000000
VERSION     DAJTMK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.327"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(20..595)
                     /locus_tag="OO135_004203"
     CDS             complement(20..595)
                     /locus_tag="OO135_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399788.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPGELASALKQQLRIAIKR"
     gene            complement(610..963)
                     /locus_tag="OO135_004204"
     CDS             complement(610..963)
                     /locus_tag="OO135_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399789.1"
                     /translation="MRDFENSFDAALARVDRTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(956..1324)
                     /locus_tag="OO135_004205"
     CDS             complement(956..1324)
                     /locus_tag="OO135_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399790.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVAEWEEEICA
                     SENEIADVDDTVHEQACRHTGEDDVGILERIRLLKCFYLCGVDDETGEPVEHVDAGRV
                     ILMPPSVAKDMVRSGMAVYA"
     gene            complement(1376..>2215)
                     /locus_tag="OO135_004206"
     CDS             complement(1376..>2215)
                     /locus_tag="OO135_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5399791.1"
                     /translation="RHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYR
                     RLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNIIQG
                     SGKEWSKQDRDTFDPTYDIDLFCDQASGLVNIAIMDGTVWRLLNGFKLFREKMDTRRG
                     SNSQLETAVKDLGAVVSFKGYYGDLVIAVAKTSYVAEDGTEKRYLPEGTLVLGNTAAE
                     GIRCYGAIKDAQALSEGVVASSRYPKHWLTVGDPSCEFTMTQSAPLMVLPDPDEFVVV
                     QVK"
BASE COUNT          496 a          686 c          493 g          540 t
ORIGIN      
        1 gcacggatat cagtgtgttt catcgtttta tcgcaatcct cagttgttgt ttcagcgcgg
       61 atgccagttc tccgggcagg cgttcacgcc ggatacggtc aacattctca tcaaatgcct
      121 gtttcagtgg ggccgccatc gggattttca ccacctgaat gggaaggcga ttacgctttt
      181 tccttccctt gtcgtcattg ccctcctcat atctggcctg gggaagacgt tgcataacat
      241 gccagcgccc attatttaat cgctggataa atgcccgctg ataacgatgc tgaccggctt
      301 tgagtatgct gttcggacga cgccccagca ttctgatccc cagcttaatc acagggagat
      361 caccgcggtt aacgataatt ctggcattcg gatttctgac cgtcgcccgt ttcagtctgg
      421 accgttcctt taccagtttc cgtctcaccc tggtttcccg ggcaacctgt gacgaagact
      481 gattaatcgc cgttgtggcc acgcggttaa tggtcattgc agaagcagcc ggaatggcgt
      541 ttttacgaac ccggctcaga ttgtcaatcg cctgatcaag cccttttatc gccataattc
      601 accctgcgtt tatcgtcgcc ggttaactgc gggtggttgc ccacggttga gccagagata
      661 acagctaccc ccgtcatccg gagaaacacg atccacccag aatgtctcac cattaatggt
      721 cagcgtgtca ccacgccgca cggcacgaac cgtatccgtc cgcacaaata atgacgggct
      781 gcttccttca atacggaccc cgctaccggc aaaccccagc gactccggat cgtcaaaaac
      841 cccctgaact tcgctgccac actgtgcccc cgaggtgaac tgcgcacaga gccccatcac
      901 ttcaacaatc gttctgtcca ccctggcaag ggcagcatca aaggaattct caaaatcacg
      961 cataaacggc cattccgctc ctgaccatgt cttttgccac tgaggggggc atcagaatta
     1021 ctctgccagc atcaacatgc tcaacaggct cacctgtttc atcgtcaaca ccgcacaggt
     1081 aaaaacactt cagaagcctg atacgttcca gaataccaac gtcatcctca ccggtgtgcc
     1141 tgcatgcctg ctcatgaacg gtatcatcaa catctgcgat ttcattttcc gaggcgcaaa
     1201 tctcctcttc ccactctgcc acacgctgtg cgatatccgc agcactcccc gacatatccg
     1261 cctcgcgccc cagcagacca gccagttcac gaagacgttt ctgattttct tcttttgttg
     1321 ccatatccag ctccctgtga aaaaaacacg ggggcatttc gcccccgctc acggattatt
     1381 tcacctgtac caccacaaac tcatccggat ccggcagcac catcagcggc gcggactgcg
     1441 tcatggtgaa ttcacaggac ggatcgccca cggtcagcca gtgtttcggg taacgggaag
     1501 aagccaccac tccttcagac aacgcctgtg catccttaat ggctccatag caacgaatcc
     1561 cctctgctgc cgtattcccc agcaccagcg tgccctccgg cagataacgt ttttcggtac
     1621 cgtcctctgc cacataagac gttttcgcta ccgcaatgac cagatcgccg taatacccct
     1681 tgaaggatac cactgcgccc agatctttca ctgccgtttc gagttgtgaa tttgaaccgc
     1741 gacgggtatc cattttttcg cggaaaagct taaagccatt cagcagacgc cagacggtac
     1801 cgtccataat ggcaatattc acaagaccac tggcctgatc acagaaaagg tcgatatcat
     1861 atgtcgggtc gaacgtgtca cgatcctgtt ttgaccactc cttaccgcta ccttgtatga
     1921 tgttattctt cgtcgatcgg ccaaaatcga cttcaatttt ctcgaactgg tctccttcca
     1981 tcgtatattt gccatacagc acggcattca ccgcctgcat ttcttccacc tgcacaatag
     2041 cgtgctcttc ctgtttgagg ttatcagtga tgatacgcag acgacggtag gccgggtcgt
     2101 tcagctgagc cggatcttca ccgggaaggc gctcaaccgc ctgctggtaa ttaaattcgt
     2161 gtttgggctt gacgtagccc ggacgcaaca cgcgggtttc accaccacga tggcg
//
LOCUS       DAJTMK010000321         2198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTMK010000321 DAJTMK010000000
VERSION     DAJTMK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.328"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..1643)
                     /gene="fimD"
                     /locus_tag="OO135_004207"
     CDS             complement(<1..1643)
                     /gene="fimD"
                     /locus_tag="OO135_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5399792.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNAASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWCLRDNTSWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDICDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNTQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGTLGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSG"
     gene            complement(1710..>2198)
                     /gene="fimC"
                     /locus_tag="OO135_004208"
     CDS             complement(1710..>2198)
                     /gene="fimC"
                     /locus_tag="OO135_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5399793.1"
                     /translation="KDGRFIVTPPLFAMKGKKENTLRILDATNNQLPQDRESLFWMNV
                     KAIPSMDKSKLTENTLQLAIISRIKLYYSPAKLALPPDQAAEKLRFRRSANSLTLINP
                     TPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALTPKMTGV
                     ME"
BASE COUNT          579 a          526 c          490 g          603 t
ORIGIN      
        1 ctaccactca aatacagtgt tgatgtgcgc ccgagttgct gagtaacggt gagctgtaat
       61 ttcccgcgtt tgttataagc gaggttgtaa tagtcggtga atttcggctt aacctgaata
      121 actccatcct gtgtttcgat gttgtagcca ttcattcgac tgtatgttgt atcagcgaaa
      181 ttaaaatatc cgctggtcga ataacggtaa cccactaact gaatattcgt gcctgactca
      241 ttgagcgatt tgttatagag aaaacgcacc gattgtccgt catgctgact gtcatcggga
      301 agtgtggaat tagcctgcgt catatccaca gacagagcgc ccagtgtccc catgtttttc
      361 ccgataccga aattaaaagc acgataacga tccgccagtt gcgttccacc atatattgtc
      421 cagccagccg gaaggccgtg gagtaatgta ctttggaaaa agcggggttt ttcctgctgc
      481 gtatttccac tacggtattc tcctgccgta atggaataac gagtatgccc ttcacgttgc
      541 aaaagcggga ctgacgaata gggtacggta aaaatctgcg tgctgccgtc agcctctttg
      601 atcgttacct gcaagtcacc actattacct gcggcataga tatcgttgat ggtaaaaggc
      661 cccggtggca ccgtactatt ataaatgtca tacccatttt gtttaatagt gacctgtgca
      721 gtaccacgag caataccgtg gatcaccggg gcaaatcctc tttggctatc gggtaacata
      781 ttgtcatctg aggccaattg tgcgccgcga aagttaatac catcgcaaat atcgccctgg
      841 gtataaccat cacccagcgt cagccgggaa cgtaacggta ttatgtctcg ctcaagccag
      901 gtattgatat gctgccattt atttttgcta cctgatgatc tgtcgctact gttataactc
      961 cagctggtat tgtcgcgtaa acaccacgca ccaatattta acccactctg taggtttaaa
     1021 tatgcataat ggctgttacc cccaatccga ttctgtacac tatttccgct gaaattataa
     1081 ttgagcaatc cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg
     1141 cgattactca taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga
     1201 tgcgcagtag cgtcatggat cattgaggtt aatggcacgc aggcatcatc tgccagcaga
     1261 ttcataccgg agacagaagc cgcattcagc cccatactgg caagttgcgc gcgtgtcagg
     1321 cagggaacaa tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata
     1381 taaccattat tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt
     1441 tcaaaacgcg ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cggattaaaa
     1501 tagagttcgg cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagccgg
     1561 acaaaaaaac cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa
     1621 agtcttaaat tcagatatga cattattcct ccggtttaaa attacccggc agtaaattct
     1681 ttttattcag gcgagaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta
     1741 agtgcgccat aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat
     1801 ttaaccgtgc tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca
     1861 ttcaactctg ttaccgtcag ataatagggt gtcgggttaa tcagcgtcag agaattcgcg
     1921 ctacgacgaa atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg
     1981 ctatagtaca gtttaatgcg gctgataatt gcgagctgta gcgtattctc agtcaatttt
     2041 gatttatcca ttgacggaat cgctttaaca ttcatccaga ataaactttc ccggtcctgt
     2101 ggcaattggt tatttgttgc atcaagaata cgcaaggtat tctctttttt tcccttcatc
     2161 gcaaacagag gaggcgtcac gataaaacga ccatcctt
//
LOCUS       DAJTMK010000322         2198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTMK010000322 DAJTMK010000000
VERSION     DAJTMK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.329"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..597
                     /locus_tag="OO135_004209"
     CDS             1..597
                     /locus_tag="OO135_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5399794.1"
                     /translation="MVYLSNNADKSISVFSKEEKIPYIIQAWVDPFNKEDKRKAPFTV
                     IPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKT
                     RIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMI
                     RPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            622..2198
                     /locus_tag="OO135_004210"
                     /pseudo
     CDS             622..2198
                     /locus_tag="OO135_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          606 a          490 c          528 g          574 t
ORIGIN      
        1 gtggtttact taagtaataa tgctgataaa tctatttctg tatttagcaa agaagaaaaa
       61 ataccttata tcatccaagc ttgggtagat ccatttaata aagaggataa aagaaaagcg
      121 ccgtttaccg ttattccgcc agtatcacga ttagaacctt cgcaagaaaa aatattacgc
      181 attattcaca ccaaaggtgt gtcattgcct gatgatcgcg agtcagtctt ctggctgaat
      241 attaaaaata ttccgccatc agcgagcaac aaagcgacga actcgctgga aatagcagta
      301 aaaacgcgta tcaagctgtt ctggcgacca gcaaatatcc gccttattcc cgaagatgct
      361 gcgccgaaag tgaaatggcg ccgggaaggg cgaaatctga tagcagagaa tcccaaccca
      421 attcatatca gtgtgatgga tgtcatcgtt gatggccatg atgtgcccct caatatgatc
      481 cgtccttttg aaacgctcac gttaccttta cctgctaatt ctgcgggagg ccaaatgacc
      541 tggcgattta ttaatgatta cggcgcagtc agtgacccga taaaaatgac gctgtaagca
      601 agggataagg ataggcgtat tatgatgacg accagaatag tggttggcct cacggcaggg
      661 acgtgtctga ttttctcgca aaacctgatg gccgaggtca gtgtattcaa tccggcgctt
      721 ctggaaatcg accatcaatc cggagtcgat attcgccagt ttaatcgggc aaacctgatg
      781 cccccaggtg tttatagcgt tgatattttt atcaacggta aaatgtttga acgtcaggat
      841 gtgacatttg ttcagaataa tccagatgct gatctgcacg cttgctttat tgccattaaa
      901 aaaacactgt cctcctttgg cataaaagtt gatgcgctaa tgatgtggat gagacggttt
      961 gcctcgatcc tgctccacgt attgaaggct catcctggca gtttgacagt gataaattgc
     1021 agctgaatat atccattccc caaatctaca tggacgcgat ggcttatgat tacatcagcc
     1081 ccacgcgttg ggatgagggg attaacgcgc tcaccatcaa ctacgatttt tctggttcac
     1141 atacactacg ttcagattat ggttcacaag agacagatac cagttatctc aatctgcgca
     1201 atggactgaa tattggaccg tggcggctac gtaattacag tactttaaac accagcgatg
     1261 gccgtgcgga atacaactcc attagtacct ggatacagcg cgatattgcc gcgttaagaa
     1321 gccagattat gattggtgat acgtggacgg cgagcgatat tttcgacagt acgcaaattc
     1381 gcggcgcgcg tttgtatact gataacgata tgctacccgc cagccagaat ggctttgctc
     1441 ctgtggttcg tgggattgca aagtccaacg ccaccgtcat cattcggcag aatggctacg
     1501 tgatttatca gtctgccgtt ccagaaggtg cttttgagat caccgatctc aacaccgcca
     1561 gtacaggtgg cgatttggac gtaaccatca aagaagaaga cggtagcgaa caacgattca
     1621 cccaacctta tgcttcattg gcgattctta aacgtgaagg tcagacagat gttgatgtca
     1681 gcgtgggtga attgcgcgat gaagacggat ttacaccgga cgtccttcag gcgcaaatac
     1741 ttcatggttt ttcccacggg atcactttat atggaggtat gcaggctgct gaaaattatg
     1801 gttctgcagc tctgggtgtc ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg
     1861 tgacacatgc tcgtgcgaat tttagccatg atgatacaga aacgggtcag tcatatcgct
     1921 ttctctattc aaaacgattt gacgacacag acactagctt gcgcctggtt ggctatcgtt
     1981 actccaccga gggctactat accctcaatg agtgggcatc gcggcgcaac agccctgaag
     2041 acttttggga aacaggtaac cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg
     2101 ggagagatta tggcaattta tacctgacat taagccggca acaatactgg cataccgatg
     2161 atgtcgaacg attaatgcaa tttggctaca gcagtagc
//
LOCUS       DAJTMK010000323         2192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTMK010000323 DAJTMK010000000
VERSION     DAJTMK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.330"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..147)
                     /locus_tag="OO135_004211"
     CDS             complement(<1..147)
                     /locus_tag="OO135_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399795.1"
                     /translation="MIKNIKTIAEAKAKCLNQKDGKQKYVVVEQWLKMFKKWYSPSGN
                     EYTAT"
     gene            complement(294..476)
                     /locus_tag="OO135_004212"
     CDS             complement(294..476)
                     /locus_tag="OO135_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399796.1"
                     /translation="MKWFIPEHVVEAFKKGELTRHQVVMNRNMARSRGYPEREKCFDD
                     ALKIIDELRKAEAEKE"
     gene            1256..2192
                     /locus_tag="OO135_004213"
                     /pseudo
     CDS             1256..2192
                     /locus_tag="OO135_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          595 a          468 c          470 g          659 t
ORIGIN      
        1 tgtagcggtg tattcattac cagatggtga gtaccatttt ttaaacatct tcaaccattg
       61 ctcaacaact acatattttt gtttaccatc tttctgattt agacatttgg ctttcgcttc
      121 ggcaattgtt ttgatatttt tgatcattta gttcctcctt tgtaaggact gtaattagta
      181 gttcattaac aagttttgga attgatttcc cttctgcctt tgcgcgattt aacaacgcgt
      241 tataagttgc agagtcaaaa ttaatattca tttggctttc tcctctcctg ttattattct
      301 ttctctgctt ctgccttacg cagttcatca ataatcttga gtgcatcgtc aaagcatttt
      361 tcacgctctg gataacctcg actccttgcc atattacgat tcattactac ttgatgtcgt
      421 gttagctcac cttttttaaa tgcctctacg acgtgttccg gtataaacca tttcataaaa
      481 aactccttat tcagttaaaa gaaaaacacc ttgcaagcct gacggcttca aagtgcattg
      541 attttgatta tttagagaag aatgtttttc tgtccctcta gtacctatta ttacatataa
      601 ataaacattt gtcaatatct tttcataaaa aatcagaact ttttcatgta cttacatttt
      661 tccttagcga ttcgtgctct cctgtgtagt gctattcttc attaccagct aaaacgctct
      721 aaaacgcgtt ctaacgaagt tttgataaaa gcaatacatt ctcttatgta atggctgttt
      781 agctcgccag aggcttattt aagtggctta ttttgtattt gttagatgat gatagatata
      841 aaacagagag ttcttagatt agttacttta taaaacgctc tgtaaagccc tgtagtgagt
      901 ttatatatgt aggcaaagcg gtttccattc tctaccctgc ttgatgttgt tagagaagca
      961 tttagacccc ttatttttaa ttacctaaag tatcctctat acatctctgt tatgaaacac
     1021 ctctgaatcc ttcataaaat acattatgac gcaaagaaca cttttctaag gtgattccct
     1081 tcctgaactc tgtttcttgc gtcagagagc tacacagaga gcttatgaag ctattattaa
     1141 taataaagta gataaagtaa gtaagataag taagaagatt aactaagata gataagatta
     1201 ataagatcat agactggccc cctgaatctc cagacaacca atatcactta aataagtgat
     1261 agtcttaata ctagttttta gactagtcat tggagagcag atgattgatg tcttaggacc
     1321 ggagaaacgc agacggcgta ctacacagga aaagatcgct attgttcagc agagttttga
     1381 accgggaatg acggtctccc ttgttgcccg gcaacacggt gtggcagcca gccagttatt
     1441 tctctggcgc aagcaatacc aggagggaag tcttactgct gtggccgcag gagagcaggt
     1501 cgttcctgcc tctgaacttg ctgctgccat gaagcagatt aaagaactcc agcgcctgct
     1561 cggcaaaaaa acgatggaaa atgaactcct taaagaagcc gttgaatacg ggcgtgcaaa
     1621 aaagtggata gcgcacgcgc ccttattgcc cggggatggg gagtaagctt agtcagccgt
     1681 tgtctccggg tgtcgcgtgc gcagttgcac gtaattctca gacgaaccga tgactggaaa
     1741 gatggtcgcc gcagccgtca ctcagatgat acggatgtgc ttctccgtat acaccatgtt
     1801 atcggagagc tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggcc
     1861 gaacttgatg gtatgcctgc tatcaatgcc aaacgtgttt accggatcat gcgccagaat
     1921 gcgctgttgc ttgagcgaaa aaccgctgta ccgccatcga aacgggcaca tactggcaaa
     1981 gtggccgtga aagaaagcaa tcaacgatgg tgctctgacg ggttcgagtt ccgctgtgat
     2041 aacggagaaa aactgcgggt cacgttcgcg ctggactgct gtgaccgtga ggcactgcac
     2101 tgggcggtca ctacgggcgg cttcgacagt gaaacagtac aggacgtcat gctgggagcg
     2161 gtggaacgcc gcttcggcag cgagcttccg ac
//
LOCUS       DAJTMK010000324         2189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTMK010000324 DAJTMK010000000
VERSION     DAJTMK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.331"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..840
                     /locus_tag="OO135_004214"
     CDS             <1..840
                     /locus_tag="OO135_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5399797.1"
                     /translation="RHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYR
                     RLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIELA
                     DGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRG
                     SNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAAE
                     GIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVV
                     QVK"
     gene            892..1275
                     /locus_tag="OO135_004215"
     CDS             892..1275
                     /locus_tag="OO135_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5399798.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVMVPSPLATAMIQHGTAEHA"
     gene            1268..1621
                     /locus_tag="OO135_004216"
     CDS             1268..1621
                     /locus_tag="OO135_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399799.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQRGSEVQGVFD
                     DPESLGFAGGGGRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRISPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1637..2170
                     /locus_tag="OO135_004217"
     CDS             1637..2170
                     /locus_tag="OO135_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399800.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
BASE COUNT          512 a          514 c          686 g          477 t
ORIGIN      
        1 cgccatcgcg gtggtgaaac ccgcgtgtta cgtccgggct acgtcaagcc caaacacgaa
       61 tttaattacc agcaggcggt tgagcgcctt cctggtgaag atccggctca gctgaacgac
      121 ccggcctacc gtcgtctgcg tatcattacc gataacctca aacaggaaga gcacgccatt
      181 gtccaggtgg aagaaatgca ggcggtgaat gccgtgctgt atggcaaata caccatggaa
      241 ggggatcagt ttgatactgt cgaggtggat ttcgggcgct ctgaaggaaa taacattgag
      301 ctggctgacg gtaaaaaatg gtctgagcag gaccgtgata cgtttgatcc gacgcatgat
      361 attgacctct actgcgatca ggccagcggc cttgtgaata tcgccattat ggacggtacg
      421 gtctggcgtc tgctgaatgg ctttaagctg ttccgcgaaa aactggatac ccgtcgcggc
      481 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc cttcaagggg
      541 tattacggcg atctggccat tgtggtggcg aaaacgtctt atgtggcaga ggacggtacc
      601 gaaaaacgtt atctgccgga gggcacactg gtcctgggga atacggcagc agagggcatt
      661 cgttgctatg gtgccattca ggatgcgcag gcgttgtccg aaggtgtggt ggcctcttcc
      721 cgttatccga aacactggct gactgtgggc gatccggccc gtgaattcac catgacgcag
      781 tccgcaccgc tgatggtgct gccggatccg gatgagtttg tggtggtaca ggtgaaataa
      841 tccgtgagcg ggggcgaaat gcccccgtgt cttttttcac aggaggctga gatggcaaca
      901 aaagaagaaa atctgaatcg tcttcgtcaa ctggctggcc tgctggggcg cgaggcggat
      961 atgtcgggga gtgctgcgga tattgctcaa cgtgtgtctg agtgggaaga ggagcttgct
     1021 gtttccccgg agggcattat gcactctgat gagagcgggg ctgatcaaaa tcacacagac
     1081 gatggtgagc agttgaacaa cacggatgct acggatgatg ttaaagcggt ccgtgtgcgg
     1141 aaatgcctgc atgtgatggg gtattgcccg gagacaggcc gtcccgttga actgacgtac
     1201 cggggcatgc gtgttatggt gccatcacca ctggcgacag ccatgataca gcacggaacg
     1261 gctgagcatg cgtgattttc agaatgcctt tgatgctgcc cttgccgggg tggacagcac
     1321 gattgttgaa gtgatggggc tctgtgcgca gttcacctcg ggagcacagc gtggaagcga
     1381 agttcagggg gtttttgacg atccggagtc gctgggtttt gccggtggcg ggggccgtat
     1441 tgaaggaagc agcccgtcat tatttgtgcg gacggatacg gtgcgtgccg tgcggcgtgg
     1501 tgacacgctg accattaacg gcgagatgtt ctgggtggat cgtatttctc cggatgacgg
     1561 gggcagctgt tatctctggc tcaaccgtgg tcaaccaccc gcagttaacc ggcgacgata
     1621 aacgcagggt gaaattatgg cgataaaagg gcttgatcag gcgattgaca atctgagccg
     1681 ggttcgtaaa aacgccattc cggcggcttc agcaatggcc attaaccgcg tggccacaac
     1741 ggcgattaat cagtcttcat cacaggttgc ccgggagaca aaggttcgcc ggaaactggt
     1801 taaggaacgg tccagactga aacgggcgac ggtcagaaat ccgaatgcca gaattatcgt
     1861 taaccgcggt gatctccctg tgattaagct ggggatcagg atgctggggc gtcgcccgaa
     1921 cagcatactt aaagccggtc agcatcggta tcagcgggca tttattcagc gattaaaaaa
     1981 tggtcgctgg catgtcatgc agcgtgtggc cgggaaaaac cgttacccca ttgatgtggt
     2041 gaaaatcccg atggcggccc cactgaaaca ggcatttgat gagaatgttg accgtatccg
     2101 gcgtgaacgc ctgcctaaag aactggcata cgcgctgaaa caacaactga ggattgcaat
     2161 aaaacgatga aacacactga cattcgtgc
//
LOCUS       DAJTMK010000325         2148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTMK010000325 DAJTMK010000000
VERSION     DAJTMK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.332"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..164
                     /locus_tag="OO135_004218"
     CDS             <1..164
                     /locus_tag="OO135_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HCR5399801.1"
                     /translation="SAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAK
                     ALKSVLARI"
     gene            complement(426..>2148)
                     /locus_tag="OO135_004219"
     CDS             complement(426..>2148)
                     /locus_tag="OO135_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5399802.1"
                     /translation="HVNDQFGNPILNESVTFSAEPPEHMTISQNIVSTDTHGIAEVTM
                     TPERNGSYMVKASLANGSSYEKDLVVIDQKLTLSASSPLIGVNSPTGATLTATLTSAN
                     GTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQ
                     TQTIVKVTGNSSTAHVASFIAAPSTIAATNSDLSTLKATVEDGSGNLIEGLTVYFALK
                     SGSATLTSLTAVTDQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQ
                     SVLKNNRSSLKGDFTDSAELHLVLHDISGNPIKVSEGLEFVQSGTNVPYVQVSAIDYS
                     KNFSGEYKATVTGGGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDL
                     PTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTYKNVGSNWE
                     RITATPKSGGPSYVYEIRVKSWWVNAGDAFMIYSLAENFCSSNSYTLPRADHLNHSRS
                     RGIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPANSNEQYVVSLATGDQSVFEKLGFAY
                     ATCYKNL"
BASE COUNT          542 a          501 c          501 g          604 t
ORIGIN      
        1 caagtgccta taataccccc gttgttgcaa tttatgctga ttacaaaacg cgatggcccg
       61 caatggccga tgtttcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg
      121 atgaattcgc aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat
      181 agtaatgccg atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc
      241 acagaaaacc agcctttgga tcaaagctgt actcaccgga tacgatcata gtcacaccga
      301 acgcgaatct ggcggtatcg catttttatt tattcatttt cggtgagaga agagcaagct
      361 ctatagaggg aatgtaaaaa agagtgcccc gaatggagca ctatttaaga aattataacg
      421 gaaaatcaga ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt
      481 tgatcacctg ttgccaggga aactacgtat tgttcgtttg agtttgcggg actagatgac
      541 caatacatat ttgattgaaa accagcctca gtcgtgtaat gccccatatc tccccattca
      601 ctgtacagtg acccgatgcc tcgggaacga ctatggttta aatgatctgc acggggaagt
      661 gtgtagctat tgctactgca aaaattttca gcaaggctgt atatcatgaa agcatcgccg
      721 gcgttcaccc accaactctt cacacggatt tcgtatacat agctagggcc tcctgatttt
      781 ggcgtcgccg taatcctttc ccaattgctg ccgacatttt tatatgtcac tttaccggta
      841 gcatcaacat cgacccagga ggcagagctt gaaaactcat aatcagccgc cgtttttcca
      901 ggggcaaagt tgtcattatt caactgataa tacgccccgg tgaatccctg cgaagggaat
      961 gtagttgtcg gtaggtcagt accattgact gacactgtac cgctcattat tttgtcttct
     1021 gcgcgagtga attgtattgt ggtactcaga ccagcttgat gaacaccatt caatacaggg
     1081 atcagcgttg cgataccctc tccgccgcct gtaacagtgg ctttgtactc gcctgagaaa
     1141 tttttactgt agtcaattgc actaacttgc acatagggca cgttggtacc tgactgcaca
     1201 aattccagcc cttcagaaac tttgatcgga ttgcctgata tatcgtgcag aacaagatgt
     1261 agctcagcac tatcggtaaa gtctcctttc aatgatgacc gattgttctt aaggacggac
     1321 tgcgaggtgt ctgccgggcc agccaccagc gttatatcta ctgtttgcat tccaccagcg
     1381 gtcgtgactg cgcttaccgt gacgctacct gtcatcgctc ctttcacgct tgttgtcgcg
     1441 attccgtttt gatcggtcac cgctgttaat gacgttaatg tggcagagcc gctttttaag
     1501 gcgaagtaca cagttaaacc ttcgatcagg ttaccactgc catcctcaac cgttgccttt
     1561 aaggtactta aatcactgtt ggtggcggcg atagtcgatg gagcagcgat aaagctagca
     1621 acatgggcgg tgcttgagtt gccagtgact ttcacgattg tctgtgtctg tattgttaca
     1681 ccgttatgga acgatgcagt caccgtatat gtaccgactt tattgctggt cagaacgact
     1741 ggagcctgac ctgaagagtt ggttctcact tttccgccac ttaacgtcgc accttctggc
     1801 gttacgctaa agttgatgac ctgaccctcc actggagtgc catttgcaga agttagcgtt
     1861 gccgtcagag ttgcacctgt tggggaattg acaccgataa gcgggctgga cgccgagagt
     1921 gtcagttttt gatcgattac caccagatcc ttctcataag aggatccatt cgccagggat
     1981 gctttcacca tatacgaacc gtttctttcg ggcgtcatag tgacctcggc tataccatgc
     2041 gtatcagtag agacaatatt ttggctgatg gtcatgtgct ctggtggctc tgcactgaat
     2101 gttacagatt cattaagaat cggattgcca aactggtcgt tgacgtga
//
LOCUS       DAJTMK010000326         2133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTMK010000326 DAJTMK010000000
VERSION     DAJTMK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.333"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..740
                     /gene="psuG"
                     /locus_tag="OO135_004220"
     CDS             <1..740
                     /gene="psuG"
                     /locus_tag="OO135_004220"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5399803.1"
                     /translation="IGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFVVAAGKNGAT
                     TVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTVVCAGAKSIL
                     DLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARAMAVKWQSGL
                     NGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTLFLLARVAELTGGDS
                     LKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            834..2084
                     /locus_tag="OO135_004221"
     CDS             834..2084
                     /locus_tag="OO135_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5399804.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          468 a          482 c          601 g          582 t
ORIGIN      
        1 ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaga aattgaatta ctgggtcgtg
       61 aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc ttttgttgtt gccgccggaa
      121 aaaatggcgc aaccactgtg gcttcaacga tgattattgc ggcgcttgcc ggaattaaag
      181 tatttgccac cgggggaatt ggtggtgtgc atcgcggggc ggaacatacc ttcgatattt
      241 ctgccgattt gcaagaactg gcaaatacta atgtcaccgt tgtttgtgcc ggggcgaaat
      301 ctattctcga tttaggatta accactgagt atttagaaac cttcggtgtg ccgttaattg
      361 gctatcagac taaagcgctg cctgcgtttt tctgccgtac cagctcgttt gacgtcagta
      421 ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc ggtgaaatgg caaagcgggc
      481 tgaacggtgg cctcgtggta gcgaacccga tcccggaaca gtttgcgatg ccggaggaat
      541 ctatcaatgc agccatagat caagccgtcg ccgaagccga agagcagggc gttattggta
      601 aagaaagtac actgttcctg ctggcgcgcg ttgctgagct gaccggcggt gacagcctga
      661 aatccaatat ccagctggtg ttcaacaacg ccattctggc gagtgaaatt gccaaagaat
      721 accagcgtct cgcgggttaa tttctgacgg gcagaaagcc gtcgcccgta ctcatcaagc
      781 ataccaaaac ctggcgctga cgccgggttt actggcatga agggaaaatc attatggata
      841 taatgagaag tgttgtgggg atggtggtgt tactggcaat agcatttctg ttgtcagtga
      901 ataaaaagag catcagtttg cgcacggttg gagccgcact gctgctgcaa atcgctattg
      961 gtggcatcat gctctacttc ccaccgggaa agtgggcagt agaacaggcg gcattaggcg
     1021 ttcataaagt gatgtcttac agtgatgccg gtagcgcctt catttttggt tcgctggttg
     1081 ggccgaaaat ggatgtcctg tttgacggtg cgggttttat cttcgccttt cgcgtacttc
     1141 cggcgattat tttcgttact gcgctcatca gtctgctgta ctacattggc gtgatggggc
     1201 tgctgattcg catccttggc agcattttcc agaaagctct taacatcagc aaaatcgaat
     1261 cttttgtcgc agtcacaact attttcctcg ggcaaaatga gatcccggcg atcgttaaac
     1321 cgtttatcga tcgcatgaat cgcaacgagt tgtttaccgc aatttgtagc gggatggcgt
     1381 ccattgctgg ttcgatgatg attggttatg ccggaatggg cgtaccaatt gactacctgt
     1441 tagcggcatc gctgatggcg atcccaggtg gtattttgtt tgcacgtatt cttagcccgg
     1501 ccaccgagcc ttcgcaggtc acatttgaaa atctgtcgtt cagcgaaacg ccgccaaaaa
     1561 gctttatcga agcggcggcg agcggtgcga tgaccgggct gaaaatcgcc gctggtgtag
     1621 cgacggtggt aatggcgttt gtcgcaatta ttgcgctgat caacggcatt atcggcggaa
     1681 ttggtggctg gtttggtttc gccaatgcct ctctggaaag tatttttggc tatgtgctgg
     1741 caccactggc gtggatcatg ggtgtggact ggagtgatgc caatcttgcg ggtagcctga
     1801 ttgggcagaa actggcgatt aacgaattcg tcgcttacct gagtttctcc ccatacctgc
     1861 aaacgggcgg cacgctggaa gtgaaaacca ttgcgattat ctcctttgcg ctttgtggtt
     1921 ttgctaactt tggttctatc ggtgttgtcg ttggcgcatt ttcggctatt tcgccaaaac
     1981 gcgcgccgga aatcgcccag cttggtttac gggcgctggc agcagcaacg ctttccaacc
     2041 tgatgagtgc gactattgcc gggttcttta ttggtttagc ttgagtttca ttgccggatg
     2101 tacgcgctta ttaagcgttg catccggcaa tcg
//
LOCUS       DAJTMK010000327         2130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTMK010000327 DAJTMK010000000
VERSION     DAJTMK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.334"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO135_004222"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO135_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5399805.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTINEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OO135_004223"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OO135_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1937
                     /locus_tag="OO135_004224"
                     /pseudo
     CDS             1519..1937
                     /locus_tag="OO135_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2011..>2130
                     /locus_tag="OO135_004225"
     CDS             2011..>2130
                     /locus_tag="OO135_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399806.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMT"
BASE COUNT          675 a          393 c          430 g          632 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatactgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcaat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tatcaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtcaaaagtg aagtggcaca ctgaatttgg ccacctgaac agaggtgata
     1981 tgctcacctc agaacaacac aggtgctcca atgaaaaaaa gaaattttag cgcagagttt
     2041 aaacgcgaat ccgctcaact ggttgttgac cagaaataca cggtggcaga tgccgccaaa
     2101 gctatggatg ttggcctttc cacaatgaca
//
LOCUS       DAJTMK010000328         2105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTMK010000328 DAJTMK010000000
VERSION     DAJTMK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.335"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..833
                     /gene="copD"
                     /locus_tag="OO135_004226"
     CDS             <1..833
                     /gene="copD"
                     /locus_tag="OO135_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR5399807.1"
                     /translation="TSLMLVFGFAMYGAWLAPLTIRRLLAKRFLRLQQHAAVWSLISA
                     TAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLALVTLIVALMQPRNMP
                     RLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAAWFGGLLPVLWCMQL
                     IKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLSALLITGFPPTLTTYWGQLLLLKAI
                     LVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAVVLVIISLLATLEPF
                     "
     gene            846..1187
                     /locus_tag="OO135_004227"
     CDS             846..1187
                     /locus_tag="OO135_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5399808.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1580..>2105)
                     /locus_tag="OO135_004228"
     CDS             complement(1580..>2105)
                     /locus_tag="OO135_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5399809.1"
                     /translation="YNCIREAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDR
                     LHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIH
                     PDGLTKKFVAARKLTGIQFSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTT
                     KMYLDEREKTYLLL"
BASE COUNT          485 a          489 c          512 g          619 t
ORIGIN      
        1 tcacctcgct gatgctggtt tttggcttcg caatgtacgg tgcctggctg gcccccttga
       61 cgattcgtcg cttgctggcg aagcgttttc tgcgtttaca acagcacgct gccgtctgga
      121 gtttgatcag tgccactgca atgctcgccg ttcagggcgg gttgatggga acgggatgga
      181 cagatgtatt ttcgccaaac atttggcagg ctgttttgca aacgcagttt ggtggcgtct
      241 ggttatggca aattgttctc gccctcgtaa cgttgatcgt cgcccttatg caaccgcgga
      301 atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg gcgggagtgg
      361 ggcatgcgac gctgaatgaa ggggtaacgg cgaaaatcca tcagactaat cacgcgattc
      421 acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg tgtatgcaac
      481 tcatcaaagg ccgttggcga caccaggcta ttcaggcgct gatgcgtttt tcatggtgcg
      541 ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct tagtgcattg ttgattaccg
      601 gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa gccatcctgg
      661 tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg cgcatgaggc
      721 aggatgaaga tcgtgcggca ccctggtttg tgtggatgac taaactcgaa tgggcgatag
      781 gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt tgatggatta
      841 cgaaaatgat gaaaaaaagt attctggcgt ttctgttact caccagttct gcagcggcgc
      901 tggctgcacc gcaggtgatt accgtcagtc gttttgaagt gggtaaagac aaatgggcgt
      961 ttaatcgcga agaggtgatg ctgacttgcc gaccgggtaa tgctttgtat gttattaatc
     1021 cgagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt gccagtggga
     1081 agaccaatgc ccaacccatt tcggtgattc agattgatga tcctaacaat cccggcgaaa
     1141 aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta cctaaaatag
     1201 ccttttgatt tccaataaaa aaaccgcctc agttctttca ccagaacggg cggtttttaa
     1261 catttcagct gatgaccacc acgcttttta ttgaccattt tgcacgcaaa ctggaaaacc
     1321 tggcgtcgtc atctattctt aaagggcaag gcaactaagc ctgcattaat gccaactttt
     1381 agcgcacggc tctctcccaa gagccatttc tctggaccga atacaggaat tgtattcggt
     1441 ctttttttaa ttgcatttga aatcaattag ttgcaaatgt ctccccgaaa ttccccgaaa
     1501 tttactcgaa tttctgtatt ccggtctttt ttggttatat cacaaccaaa atacatttaa
     1561 caatccattt acgttaaaat cagagcagta agtacgtttt ttctctctca tcaagataca
     1621 tttttgttgt cttctccgat gtgtggccaa gtagacgctg agcaaattct tctccacatg
     1681 tttctttgta caatcgtcca gccaggcttc tgacttcgtg aaaagttggt gggttttcac
     1741 tgaactggat acctgttaat tttctggctg cgacaaattt ctttgtcagg ccgtccgggt
     1801 gaatgctgcc gtcagggctg ttttttctaa tcccggcact gattagataa tcccccctac
     1861 ttaccatgcg gcactgttca actactgcac caagtcgtaa accagcgaca ggaaggctga
     1921 gtgacagggg gatagcaatc atcattcctg tcttaatttg cctgatgtgg agacgatcat
     1981 cataaatatc actgaaccgc atattcgtta tgtcttcgcg acgttgtcct gttacaaggg
     2041 ctaaatccat agctaatggg aaccatgccg gaagttgatc tgctgcctcc ctgatgcagt
     2101 tgtat
//
LOCUS       DAJTMK010000329         2099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTMK010000329 DAJTMK010000000
VERSION     DAJTMK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.336"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..684)
                     /gene="ipaH7.8"
                     /locus_tag="OO135_004229"
     CDS             complement(<1..684)
                     /gene="ipaH7.8"
                     /locus_tag="OO135_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5399810.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYR"
     gene            1012..1191
                     /locus_tag="OO135_004230"
                     /pseudo
     CDS             1012..1191
                     /locus_tag="OO135_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1208..1898)
                     /locus_tag="OO135_004231"
                     /pseudo
     CDS             complement(1208..1898)
                     /locus_tag="OO135_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1821..2072
                     /locus_tag="OO135_004232"
     CDS             1821..2072
                     /locus_tag="OO135_004232"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399811.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
BASE COUNT          620 a          389 c          469 g          621 t
ORIGIN      
        1 cctgtaactt gaatctaatt gtgaaaaact ctccggaaga gtggttatct gattaaaatg
       61 aaaataatat tccttttgtc tgatattgtt tctatcagaa aattgaggaa aagtaaccag
      121 attattgcgc tgagcacaga gaatttccag tttatccggt aagtcgggta atgttgtcag
      181 cctgttataa taaacatgta aaaatttcaa tttttcggga agagccggta agttatatag
      241 cctgttattt tcaacaaata atgatgttaa atggtttggc aaaacaggaa ggttttccag
      301 ttgattagaa cgaacactta aagttgttaa tgactcagga agttctggta tcacagaaag
      361 cctgttatta tcagcattaa gttctttgag aaatgcaggc agctttggca atgatgttaa
      421 tttattattt tctacaatca aagcagaaat atgctttggt aattcgggaa gagacgtcaa
      481 atttaggtct gataaattta atactgcttc ttgattctga aagcactggg agagtctgtt
      541 aaaagcaatg cctcgctctt ccccgggaga agagttcttt tcccattcag tcaggattct
      601 cagataatgt tcattactgg ttgagtttga gttaatagag ggggagcaag aaactgatga
      661 gtgtgtatta tttacagaga acataatgct agggcaacct tatatttgtg agaataaata
      721 tttaaaattg tatatttata caaatttcac ttaccatttc cgatattaag ttttaatagt
      781 ttccatttta cacataacag aagttagttg taatttattt attgaggttt tctatggaat
      841 tcttttttat tgtgactgca gtcaataata acactgaatt caacattcca gtgagcaagg
      901 aaatgtaatc cgagtaagta tatttgtttt acatcatatt ttagccaagc tcatttattc
      961 ctattataga atgataaatt aaaattactg taaaaaaacg atacacattt aatacgaggg
     1021 aggccggcag aaaaacggct tgcggtaagg caggagaaat gtgtgccgcg aatgaagtcc
     1081 ttgtatgact ggataaagct ccagaggaaa actttgtcaa accatgctga aatggtaaag
     1141 gcgttcaact atatcctgaa tcactggaat gcactgaatg agttttgccg ggtagtactt
     1201 ccaatcatta gaatatgtgc ttttcaatga aggaaccgat gactttttcg tggatttcca
     1261 cggaacgcga gaagcagatt gttttacgtg ccagccgctt gatgcggggg cggagggtca
     1321 ggttgttacg ttcatacgtt gcgtgaagat tttgccggtc aggtgtttct cctccggcac
     1381 ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga acggagtcaa
     1441 cagggctaac agttcccgac aggtctcatc attccgtggc ccgcaagtgt aagccaacac
     1501 accgccagtt ttagtgttgt acgcatacca gggccagtgt tgttgcgcct tgctgccaac
     1561 gaagcgccac tgctcatcaa gttcacagat aagggcaaca tcagcgtgag cgactggcgc
     1621 agatgttatc cgccggggcg agagcttttt aatgtccgta tgacggtatt gatgccgatt
     1681 ttcagtgttc tggcggtata acagccccct gcgccgttat gtgccatttc aacgatctgc
     1741 tccttaacgc cgagttttct ggcttcataa gagtaagtga gctggaaaac acgtttacat
     1801 gagcgacaac ggaaacgctc atgccgggaa caactacggc catgtcgata aacttcatcg
     1861 gaattacagc gcggacaatg aacagtaacg gtagtcatgg aaaacctcaa ataccagtag
     1921 attaccagaa acttctatcg attgaagaca ttaccttttg ccgcaatagg tggggtaaca
     1981 ttggtgaaaa cgcattacgc cgggtggcag ttggaaaaaa attatctttt ttcggctctg
     2041 atcgtggtgg agaaaatgcg gcaataatct aaatagctgc gcttaatacc gctacactt
//
LOCUS       DAJTMK010000330         2087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTMK010000330 DAJTMK010000000
VERSION     DAJTMK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.337"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..1456)
                     /locus_tag="OO135_004233"
                     /pseudo
     CDS             complement(1..1456)
                     /locus_tag="OO135_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
     gene            complement(1453..>2087)
                     /locus_tag="OO135_004234"
     CDS             complement(1453..>2087)
                     /locus_tag="OO135_004234"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR5399812.1"
                     /translation="VLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRG
                     LCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVVY
                     CDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYCNFTH
                     HYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT          503 a          559 c          502 g          523 t
ORIGIN      
        1 aatgttctta ctgatgtatt tcgcgatgta gcttgtcggc gttcctttgc gcgggtttat
       61 cagctcatat ttaaagcgtg gaccagtgtt attacccagt tcctcgcggt cttcacggat
      121 ggcaaactta cgcaacaatg cagtaatggc gcggcggtct tttttgcgca taaaacacaa
      181 caggtgccag tgaactgtac cgtcatgatg cggctcagcc acccgcacgc cataccagcg
      241 caacccggct ttgtgcatcg ccttacgaaa tgcagcaaac atgccgacca gataatcgct
      301 gctttgtctt accgtcgcat ttgtccaggt tgggtttggt ctgccgttat tgagcgtgga
      361 atggaaacgt gacggacagg taatggtgta gaaaacggcg cagtcaccgc gcatttccgc
      421 gataagctcc agacctttaa cacaggccat catctcattg cggcggtgcg ccgggttgct
      481 gctgctggcg tttaccacgt cttccatatc cagcgtgtcg ccctcttcgt tcaccagttc
      541 atgagaacgg aaaaactcca atgacttgcg gcgctgctca cgtttatgca tcacggcttc
      601 atagctgaca tagggagatg cttttttgct gaccaggcag acagcacgca actgctcttc
      661 ccgccattcg caacgcattt tccataattt ccggtaccac cagtcggcgc acaacatacg
      721 cgccagcgaa cccggaatga gttcataggg cacgggttta cggcggtttc ttttccgacg
      781 gagttgctca aacgcaggcg ggatgacatc cagacgcagg gtttccgctg ccaccttttc
      841 ccatgtcttg cggatttctt ctggcttaac gtcatcggtg gcatacaaat caccacaagc
      901 tgcatcaagg cacatgctca tatgcgcagc gacaagggta gacaggcgtt tcacctgatc
      961 ctgactcatt tcaggcagaa tcagcaggcc gtccagccct acatggcttg ccataaaacg
     1021 aaaagatgca gatagctggc tgtcgcgtac atgctccagt cgttccagac atggcttaat
     1081 cgtctcacgt aaatagcggg aataagcctt tggcctgccc aggctgctga agtattcaat
     1141 acgttgcatc agcggcttgc tgatatggga aggctgggcg ttgacgtccg ccagaatgac
     1201 catatccgga ttaaaacgct gctgctcatg cgccagcttt gcccggctaa tgagcttatc
     1261 ctgctccatt tcgtgctgga caggatcacg tgattcataa aaaaaataac gctcccagac
     1321 ctgatcactc agtgcctcgc ggcgcaactt ccgccgggtc cagataaggg ttaatggcct
     1381 ttttcgggct gttccatgag aacgctgcgg cagcctcgtt aaagccgcag cagttgttca
     1441 tatcggcatg gctcatgcac gtactccgta cacggcagaa ctatccacgc cacgcgaata
     1501 atcaaatccc acccagcagc gcggcccgga aacagcaatg atttctgttg ctgatttacc
     1561 ctcgccagct gccacaccga tgctgcgttt taccttgata tagtggtgag taaaattgca
     1621 atacagcgaa cggatcaggg atgtgtcact gttagaaaca atgaccggat gtccttctga
     1681 tgaccgatgt tcaagaacgg atgctaggtg atactggtca tcttcagtga aaccatcagt
     1741 gtgatagccg gaaaacgtac cgtcatacgg tggatcgcaa tacaccacat cccccgcctt
     1801 caacatcgcc agcgtttcat caaagctggc gcagataaac gttgctcgct gggctttttc
     1861 tgcaaatgcg cgaatttctt tttcagggaa atacggattt ttataattgc cgtagggaat
     1921 gttgaaatgc ccgctcttgt tatagcgaca taaaccacgg taaccgtgac gattgagata
     1981 caggaaatat accgctttca tgaaatcagt aatttcagtt gagtaattaa actcctgcct
     2041 tatgttgtaa taagccacct ccctgtttgc gatctcaaat aaaactc
//
LOCUS       DAJTMK010000331         2065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTMK010000331 DAJTMK010000000
VERSION     DAJTMK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.338"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..1625)
                     /locus_tag="OO135_004235"
                     /pseudo
     CDS             complement(1..1625)
                     /locus_tag="OO135_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
     gene            complement(1977..>2065)
                     /locus_tag="OO135_004236"
     CDS             complement(1977..>2065)
                     /locus_tag="OO135_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HCR5399813.1"
                     /translation="RQMQARRVLQQMSDERLRDIGLRREDVE"
BASE COUNT          540 a          513 c          431 g          581 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagttcag
      301 gccagcaaag acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt
      361 gtcgtctggt cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct
      421 gcctgaccat ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa
      481 ttatgtaagt agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca
      541 tcgccgaaac ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca
      601 ggataggcaa atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca
      661 taagtatacg caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca
      721 tcctgggctt tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga
      781 ccattggcac caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt
      841 aacgccagca gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa
      901 tcgtagcgtg gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag
      961 ctactctctt gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga
     1021 ttaaataccg ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc
     1081 agattatcaa tttcgctagt gctatcagag aagcggtttg ccatagtgcc cacaaatatc
     1141 atcgcgacat caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt
     1201 ggtaagagcg tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg
     1261 tagccttgca gaatggacat attctctggg gaaagggcat caatttgcgc ccggatagca
     1321 tccggccagt agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact
     1381 tccgcgacag tcccttgagt actgcgacgt gccatttcca tctgaaaaag gcgatcttgt
     1441 gctactacat agccatagcc ataaaatagg tgccatgtat cattggcata aatatgcggc
     1501 atgccgtatt catcgcgaac aatctttatc tcacttgacg actgctcagc cagtgcaggt
     1561 aagctccaat aatacatcag ggaagcagta acacagttca cgatcatacg atttctattt
     1621 ttcattgtat cctctggcag gtgtataatt agcgaattga tactagcaac gaagcttcgg
     1681 ttatctattt gcagagaatt gtttcattat gaactgtgag cgcgataact aatttttgat
     1741 acaaaaattt cattaatgtg atctaaaacg aatcgcttta ttcaccgctg atgattttgt
     1801 tctattcctt tttgtgacag atcccccgga cgcatagatg atgaccccca gagagacagc
     1861 tcaggtcatt atctgtatgt aaatcagaaa aatatgttgt aaacgacttt tatggttgga
     1921 ttttgtagca attttgaatg cagacaggca gcatttgcca acacgccgcc catagttcac
     1981 tccacatcct ccctgcgtaa cccgatatcc ctcaaccgtt catcactcat ctgctgtaac
     2041 acccgtcggg cctgcatttg ccgcc
//
LOCUS       DAJTMK010000332         2037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTMK010000332 DAJTMK010000000
VERSION     DAJTMK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.339"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            299..610
                     /locus_tag="OO135_004237"
     CDS             299..610
                     /locus_tag="OO135_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5399814.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLHAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            610..897
                     /locus_tag="OO135_004238"
     CDS             610..897
                     /locus_tag="OO135_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5399815.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1435..1602)
                     /locus_tag="OO135_004239"
                     /pseudo
     CDS             complement(1435..1602)
                     /locus_tag="OO135_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            1727..1879
                     /locus_tag="OO135_004240"
                     /pseudo
     CDS             1727..1879
                     /locus_tag="OO135_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          574 a          491 c          483 g          489 t
ORIGIN      
        1 cagtaatcat gccgggtctg gctggctaac accccattat aaggggtacg aataaagtaa
       61 cgggggtaag gtgaaatctg tcaatcagta ccggcttacg ccgggcttcg gcggttttac
      121 tcctgtgcca tatgcaacaa cggagtgccg ccttccatgc cgctaacgcg gcatcaaaaa
      181 atccctcttc tccagttgcc cccctccgaa ctcagagtgt attttcattt attaatgaaa
      241 tttactgttt gcgcaatttt tctgttttag tgtattttgt cattctggag gctataatat
      301 gtttaatgtg attactcacc cggccgcact ggatgaactt ctagaattac cagacaatct
      361 gcgtggtcgt atgaccagac tgattgaacg gctggaacag gaaggtaaca aaatgaaaat
      421 gcctcacagc cgtgtaattg gcggagggct ttttgaactg agagtcgggg ataaaaatat
      481 cgcaaggaca ctgcacgctt atgcaacggg taacgaaatc tatctgctgc atgcatttgt
      541 gaaaaaaacg caaaagacac caacgaaagc tattgaaatt gccaggatgc gtctgaagga
      601 gatgaactga tgaaagtaaa aggtgttcca ttcagccagg taaagcaaaa actactcagt
      661 actccagaag ctgtcaaagg gtatgaggat gctgacaaag aactggagat ggtggaaatg
      721 ttatacgaaa tgagagaaaa agcgggatta acaaagtcag cactggccga acgaatgggg
      781 ttacgcccgt cagccatcag tcgcctggaa agtaaccctt taggggccag catgaaaact
      841 ttgacaaaat atgctcatgc ctgtggcgca accatcgata ttcacgctgt ctattgaggg
      901 gtccgcttgg aattcggaaa ataaaccacg ttaagactat tttttagata aatgactttc
      961 aggaatcgcc aagatggcgg gataacagcg gttttggtgt cctaaatcgt ataattctgt
     1021 acatccacgc aagggaatgt atggaagtaa gccagacggt tatcggacgg tcagaacatt
     1081 tcgtacagcc tttacgttca gtgagttagc tggcctggac gtaaccactc gttagctcaa
     1141 ttctcttagc gtgggaaaat ttccgaattc caagcggacc cctttatcct tgccccgctg
     1201 aatgaggaaa cgctgttccg ggggattatg ctgaacgtct tccgttcatc gataggaatt
     1261 aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac accacttttg
     1321 ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag ggtatgaatg
     1381 gcggtttttt gctccgtttc cctcaaaatg gacgccactt accctctgcg gctctcagcc
     1441 gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca gacggtagcc
     1501 ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca gcgggtcaac
     1561 cttcagtaac ctctgataca tccctctcca gggtaagcgt aaaccaggtc ctcagagaat
     1621 acatattctg cggagcgtgc ggcaacaaca ggacgaacag gggcggtttt caatttgcag
     1681 ccgtcaggct gccgtggttc actccaacaa cggagacagg taaattatgg acgaaaagaa
     1741 gcttaaagcc cttgcggctg aactggctaa aggcctcaaa accgaggccg acctcaatca
     1801 gttttcccgt atgcagacga aattaactgt cgaaacggtg cttaatgcgg aactaactga
     1861 ccatctcggg catgaaaaaa agctacatcc ggtagtaaac attatcagtg ggttatgcga
     1921 tatgatttga gccgttttca gacaatcgca gggccagtac ctcgtcagtc aactgacggt
     1981 aaatctgctg ttcagtctca cgcatcacct gtgcaccggc ttccctctcc gcatccg
//
LOCUS       DAJTMK010000333         1986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTMK010000333 DAJTMK010000000
VERSION     DAJTMK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.340"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1668
                     /locus_tag="OO135_004241"
     CDS             <1..1668
                     /locus_tag="OO135_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HCR5399816.1"
                     /translation="PETYDRPDPRIKQLARRSTVTPGGASCNYNDIIPADHCLHDVQD
                     LSKFSHPKASAAQYGCVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFLAGDEGTFS
                     ESTGASETSARWGVDKPLYKDLLTRTQAALKANPKNILLAVVWMQGEFDLKQGAYATQ
                     PGLFDSMVEKYRSDLSEFGGQCLGGSPSSVPWICGDTTYYWKQTYSSQYDAVYGAYKT
                     KSAKKIFFVPFMTDENGRNVGTNEPSEDPDVADIGYYGAGGRTDAKTWTTADRKTHFG
                     SWARRGIISDRLATAILVHAGRTAEFITGKQPDTVKPTGPSGEGTEREPEAPVSNRTL
                     MSLLASGEDLASQGWRYYHKPASGDNVNKNIAEAVVSDAGATGGKALQLNKPENHIWF
                     LEHDAAGQGVELLKKGGRVSVRFKLPGSLMPNRFALGIYWQLSSLPEGVTLAEEGNDM
                     LMSFFLQTDATNLNAMYHKKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKR
                     GTPFTLVKSPASGAADKLQLTGISKAATYTLLIDSVKVEVNNADAAA"
     gene            1804..1938
                     /locus_tag="OO135_004242"
     CDS             1804..1938
                     /locus_tag="OO135_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR5399817.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     "
BASE COUNT          490 a          454 c          598 g          444 t
ORIGIN      
        1 ccggagacat atgaccgtcc ggacccgcgt attaaacagc tggcgcgtcg cagcactgtc
       61 acgccgggtg gtgcgtcctg taactacaat gacattattc ctgcggacca ctgcctgcat
      121 gatgttcagg atttgagtaa gttttcacac ccgaaagcca gcgcagctca gtatggatgc
      181 gtggggcagg gattacatat cgcgaagaaa ttgttgccgt ttattccggc gaatgccggt
      241 attcttctgg ttccgtgctg ccgtggtggt tctgcatttt tggcgggcga tgaaggtacc
      301 ttcagcgaat ccaccggcgc aagcgagacc tcggcacgct ggggtgtaga taagccactg
      361 tacaaggacc tgcttacccg tactcaggcc gcactgaagg ctaaccctaa aaatattctg
      421 cttgcagtgg tctggatgca gggcgagttt gatttgaaac agggtgcata cgccactcag
      481 ccggggctgt ttgattccat ggtggaaaaa tatcgttctg acctgtcgga attcggaggt
      541 cagtgtctcg ggggctctcc gtcatcggtt ccctggattt gtggcgacac gacctactac
      601 tggaagcaga cttattcttc gcaatacgat gcggtgtatg gtgcatacaa gacgaaatcc
      661 gcaaaaaaaa tcttctttgt gccgtttatg acggatgaaa acgggcgaaa tgtgggtacc
      721 aacgagccgt cagaagatcc ggatgttgcg gatattgggt attacggagc cggtggtcga
      781 acggacgcca aaacctggac gacggccgac cgtaaaacgc attttggatc atgggcacgt
      841 cgtgggatta tttccgaccg tctggcaacg gcgattcttg tgcatgccgg gagaaccgct
      901 gaattcatta ccggaaaaca gcctgatacg gtgaagccca ccggaccttc cggtgaaggt
      961 acggagagag agccggaagc cccggtcagt aaccgaaccc tgatgagtct gctggcgtcc
     1021 ggcgaagacc tggcatcaca gggctggcgc tattatcaca aaccggcgag cggagacaat
     1081 gttaataaaa acattgctga agcggtggtc agtgatgcgg gggctacggg aggtaaggcc
     1141 ctgcaactga ataaaccgga aaaccacatc tggtttctgg agcatgatgc agccggacag
     1201 ggagtggagt tactgaagaa ggggggacgt gtgagcgtac ggtttaagtt gccgggttca
     1261 ctgatgccga atcggtttgc cctgggcatt tactggcagt tgtcgtccct gccggaggga
     1321 gtgacgctgg cagaggaagg caacgacatg ctgatgtcct tcttcctgca gacggatgcg
     1381 acgaacctga acgcgatgta ccacaagaag ccgaatgcga agctggatac gttcggggtc
     1441 tttgataacg gatggcacac actggctttt gagtttgccg gaaacaacag cattcaggtg
     1501 acgccggtac tggatgagaa acgggggacg ccgttcacac tggtgaaatc tccggcatca
     1561 ggggcggcgg acaaactgca actgacaggc atatcaaagg cggcgacata tacgctgctg
     1621 attgacagtg tgaaggtgga agtgaacaac gcggatgccg cggcatgata aaaaaagccg
     1681 ccagcggcag gaacggaagc tggcggaggt aatcccaatg gagaatgtaa agaaaagatg
     1741 ctttcgtata tcggtttttt aaatgaaaac agttctcatt gtcaaccata acggtaagaa
     1801 actatgacat ttattcatca ggtgatgctg tacttctgta cggcggtctg tgtgctgtat
     1861 cttctttcgg gtgggtacag ggcagtgcgc gatttctggc gcaggcagat tgataaaagg
     1921 gccgcagaga aaatctgagg tgtactggca atagcggaca ctaccatttg ttcttttttt
     1981 aagcag
//
LOCUS       DAJTMK010000334         1971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTMK010000334 DAJTMK010000000
VERSION     DAJTMK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.341"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..327
                     /gene="tnpB"
                     /locus_tag="OO135_004243"
     CDS             <1..327
                     /gene="tnpB"
                     /locus_tag="OO135_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5399818.1"
                     /translation="RIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLFIFRGRRGD
                     MIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDWRQPKTSRL
                     NALTML"
     gene            358..1950
                     /locus_tag="OO135_004244"
     CDS             358..1950
                     /locus_tag="OO135_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5399819.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
BASE COUNT          495 a          495 c          569 g          412 t
ORIGIN      
        1 cgcatctggc tcgttgctgg gataaccgat atgcgtaagt ctttcaacgg gctgggtgaa
       61 caggtacagc atgtgctgaa tgataatccc ttctccggtc acctgttcat cttccgtggc
      121 cgacggggtg acatgattaa aatcctgtgg gctgatgctg atggtctgtg cctgttcacc
      181 agacgcctgg aggaaggcca gtttatctgg cctgctgtgc gtgacggcaa ggtatccatt
      241 acccgctcgc aactggcaat gctcctcgat aagctggact ggcgtcagcc aaaaacatcc
      301 cgccttaacg cactgacaat gttgtaaaaa tgtcatggcc ggattataaa aacggccatg
      361 aatcaaaaat acctcattcg cattgcagaa ctggaatgcc agctccgtca gaaagaccag
      421 caactgagtc tggttgaaga gacggaggcc ttcctgcgct ctgcactggc ccgcgccgaa
      481 gaaaagatcg aagaagatga acgggaaata gaacatctgc gggctcagat agaaaaactg
      541 cgccggatgc tgttcggtac ccgttctgaa aaactgcgtc gtgaagttga acaggctgag
      601 gccctgctga aacaacgcga acaggacagt gatcgttaca gtgggcggga agacgatccg
      661 caggttcccc gccagttgcg acagtctcgt catcgtcgcc cgttaccgga gcatctgccc
      721 cgcgaaataa atcgcctgga gccagaagaa agctgttgcc cggagtgtgg cggtgagctg
      781 gattatctgg gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc tctgaaagtg
      841 atccgcacag aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg
      901 gcaccatccc gtccgataga gcgtggtatc gcgggcccgg ggttacttgc ccgcgtgtta
      961 acgggaaaat actgcgaaca cctgccactg tatcgtcaga gtgaaatttt tgcccgtcag
     1021 ggtgtcgaac tgagccgtgc attactctcc aactgggttg acgcgtgctg ccagttaatg
     1081 acgccgctga atgatgctct gtaccgttat gtgatgaaca gccgcaaagt tcacactgat
     1141 gacacaccag taaaagtgct ggcaccgggc aggaagaagg cgaaaacagg atatatctgg
     1201 acgtatgtcc gggatgacag gaatgccggt tcgccagagc ctccggcggt ctggttcgcc
     1261 tactcaccgg accatcaggg taaacatccg gagcagcacc ttagtccctt ccggggtatc
     1321 ctgcaggcag atgcgtttaa tggttacgat cggctgttca gtgccgaacg agaaggcggc
     1381 gcgttgacgg aagcaggatg ctgggctcat gcgcggcgca aagtccacga tgtatatatc
     1441 agtaccaaaa gcgcgacagc ggaagaagcc ctgaaactaa tcggtgagct gtacgccatc
     1501 gagcacgaaa tacgcgggtt gccggtgtct gaacgcctgg cggtcaggca aatgcagagt
     1561 aaaccgctac tgacttccct gtataagctg atgcaggaga aagaacacac gttatcgaaa
     1621 aaatgccgtc tgagagatgc gttccggtat atcaggaagc actgggttgc gttgtgcaac
     1681 ttcagtgatg atggtctggc tgaggcggat aataatgccg cggaaagagc gcttcgtgca
     1741 gtctgtctcg gaaagaaaaa ctttatgttc ttcggcagcg atcacggtgg agagcgtggt
     1801 gcgctactgt acgggctgat cggcacctgc cgactgaacg gtatcgatcc ggaagcgtat
     1861 ctgcgctata tcctgagcgt actgccggaa tggccttcca accgtgttga cgaactcctg
     1921 ccatggaacg tagcactcac caataaataa gcgtcaatac ggtgctccgt t
//
LOCUS       DAJTMK010000335         1957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTMK010000335 DAJTMK010000000
VERSION     DAJTMK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.342"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(345..1445)
                     /gene="chaA"
                     /locus_tag="OO135_004245"
     CDS             complement(345..1445)
                     /gene="chaA"
                     /locus_tag="OO135_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5399820.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1715..1945
                     /gene="chaB"
                     /locus_tag="OO135_004246"
     CDS             1715..1945
                     /gene="chaB"
                     /locus_tag="OO135_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5399821.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
BASE COUNT          592 a          444 c          451 g          470 t
ORIGIN      
        1 attagccccc gttctgttgt caggttttac ctctcaacgt gcgggggttt tctctttcca
       61 gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg ataattccgg
      121 cttggtgtgg atactacgtc tcaattcatc ttcacttcat ccctgaaatg tttgcaataa
      181 agagtacatt ccggcttttc aacagctgtt gcagtggttt tgtgcaggtt gtggtacagg
      241 ctcgcaattc tgattaacga gctgtccagg tagtatgaag aaatacaata agcaggaatt
      301 tatcttatct cccccgattt ccgtcatcgg aggagatata actgtcaggc aaatatcgtc
      361 atcaaatagg cggcaaacag tgccagatgc gctgcgccat tgagcacgtt agtacgtccg
      421 gtggagaagg agatatggca cagcactaaa gaggccacca tcaccaccat ttctggcgca
      481 ccaagtgcaa actgtaattc gttacccgtc ataaaggcaa ttagcgtgac gacaggtacg
      541 gtaagcgaaa tggttgctaa cactgaacca aagaacagat tcatcgcgcg ctggacctgg
      601 ttgttcaaca ctgcttttaa cgcacctaaa ccttccggcg acagaatcaa cagagccacc
      661 aggaagccag taaaggcgac aggggcattc atgctgtcga gcaatgtctc cagcgggctg
      721 gcattcattt tggtgactgc aataacggca atcagatgga taatcaacca gatagcatgc
      781 cacacgctgc tatgggcaga cggtttaccg tgatgcgggt cgtcgtcatc actgtcatct
      841 tcgtgctcgt agacaaacaa actttgatgc gttttggtct ggatcagcaa aaatacgcca
      901 tacattgccg cagaaattaa tgctaccagt aacgcctgac cggttgaaaa attcgccgca
      961 ggcagagcca ttggaaatac cagtacgatt atcgccaggg ggaacagcgc aattaaatac
     1021 tgcttgatac caaacagatt catatattgg gtggcaaact tacgaccgcc caacaataat
     1081 gaaaagccaa ccagcccacc ggtaacaatc ataatgattg aatagagcgt atcacgcatt
     1141 agcgttggcg cggcgtcgcc ggttgccatt aaagctgaaa tcaaactgac ttcaagaata
     1201 accactgaaa ggctaagaat aagcgaaccg taaggttctc ccaggcgatg ggctaatacg
     1261 tccgcatgac ggacaacact aaaggcgcta cttaaaatac caataagcgc aagaagattg
     1321 atggcaatga ccactggtag tgtctggctg cttccccaca ggaacagcac taccagcgcc
     1381 agaaccggga aaataagcga agtctccttg tggcgggttt ttaccgcctc ttgagcattt
     1441 gacattatgg ttatcccttt gcagatgaat ttatcgaaaa tgtaaaaaat aggtaggaaa
     1501 aataacagaa agtgtctgga tatcggtaac attttacgaa tttttacccc actgtcgatt
     1561 ttttactcat tgggacataa aaataagtat tacgtttaga caatgtttgt tttagcgttt
     1621 cttaagaaca gtctgacatg aaaattctta tgttttggca agagtagata ttgttgacca
     1681 cacttaatgt tcaactttgt aaaaggagtc aacgatgccg tataaaacga aaagcgatct
     1741 gccggaaagc gtaaagcacg ttctaccgtc tcatgcccag gatatctata aagaagcgtt
     1801 caacagcgca tgggatcaat ataaagataa agacgatcgg cgtgatgacg ccagtcgcga
     1861 agaaaccgcg cataaagtgg cctgggctgc tgtgaagcat gaatatgcca aaggggatga
     1921 tgataaatgg cataaaaaat cgtaaaaccg gtagctt
//
LOCUS       DAJTMK010000336         1934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTMK010000336 DAJTMK010000000
VERSION     DAJTMK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.343"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            45..323
                     /locus_tag="OO135_004247"
     CDS             45..323
                     /locus_tag="OO135_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5399822.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            310..840
                     /locus_tag="OO135_004248"
                     /pseudo
     CDS             310..840
                     /locus_tag="OO135_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1174..1695
                     /locus_tag="OO135_004249"
     CDS             1174..1695
                     /locus_tag="OO135_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399823.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
     gene            1698..>1934
                     /locus_tag="OO135_004250"
     CDS             1698..>1934
                     /locus_tag="OO135_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5399824.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSEDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVTGIEPRNASVIEV"
BASE COUNT          508 a          422 c          531 g          473 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg ggcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caagctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggca
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      901 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt
      961 gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatttgcgg
     1021 acgggtgaaa tatccatgtc gcataaagtg tctgttggcg gtggttcgca ggtcaatggt
     1081 gcgctgggta tcggcgttca gaacgccctg ggggggaatt caattgttct tggtgataat
     1141 gacaccggat ttaaacagaa tggagacggt attctggatg tttatgctaa tagtcagcgt
     1201 gtattccgct ttcagaatgg agtggctatt gcttttaaga atattcaggc cggaactgcc
     1261 agaaaattca cgttatccag cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg
     1321 ggtaatccat ccaggtctgt tgttgcagag cttggcgatg attcaggctg gcattttttc
     1381 agccagagga atacagataa cagcatcacg ttcgccgtaa acggacaggt aattccgtta
     1441 aattacggaa acttcgatgc ccgctataaa tatcgaacag agggggtaca ggatgtacgg
     1501 tatggccatg aaatgtatta cagccccggc agtaacaccg tttcgtggag atttcgcgca
     1561 ccttcgggac acgggctgtc agggatagcg atatcggata ccggccgtga ctcagcggat
     1621 aacgtcaacg gcgtgtatta ccgcccgctt cagaaactga ttaatggcac ctggtataac
     1681 gtggcgagtg tttaacaatg ttgcatttaa aaaatattac tgcgggcaat ccgaaaaccg
     1741 tggaacaata tcagctgaca aaacaatatg atgtcacctg gcttttttcg gaagacggaa
     1801 aaaactggta tgaagagctg aagaatttcg ccagcgacac aattaaaata acttacaccg
     1861 gagacggtcg cgtggtgtgg gtcggtaagg atgtgacagg cattgaaccc cgtaacgcca
     1921 gtgttattga agtt
//
LOCUS       DAJTMK010000337         1889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTMK010000337 DAJTMK010000000
VERSION     DAJTMK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.344"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            737..964
                     /locus_tag="OO135_004251"
                     /pseudo
     CDS             737..964
                     /locus_tag="OO135_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(999..1139)
                     /gene="ykgO"
                     /locus_tag="OO135_004252"
     CDS             complement(999..1139)
                     /gene="ykgO"
                     /locus_tag="OO135_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCR5399825.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1139..1402)
                     /locus_tag="OO135_004253"
     CDS             complement(1139..1402)
                     /locus_tag="OO135_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCR5399826.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1766..1867)
                     /locus_tag="OO135_004254"
                     /pseudo
     CDS             complement(1766..1867)
                     /locus_tag="OO135_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          574 a          354 c          405 g          556 t
ORIGIN      
        1 catctgatgg aaatggaagc cacgcaagca ccttaaaatc accatcatac actaaatcag
       61 taagttggca gcattacccg attgggctta aatttatgtt tttttcccga tgagtgtcaa
      121 tatgtgaacg gaaaaggaat tcaatggtta agtcaatatt tagaaaaact gaaaatattg
      181 cttttttgat aagaagaaaa agacatttgg tttcataagg ttattggcaa tataacgttc
      241 aatccttact tgcattttat attggtcagg aactttgccc tgaagtgtta ggaatagtct
      301 taatgcgctg tgtgatggac cagtaaaaaa acaagtttta agggtaaggg atagaatata
      361 aaactataat gagctgtcat tgaagatgat ttgccaatca gtaagaaatt ttaatggatt
      421 aacatgccgc acaaaatggt tataggtgtt cattacaata agtgatggaa aattgaacgg
      481 gcgtatgtac gtcatctgtt cggtaagccc tcaagagcca gattaagtga agttaaacga
      541 aggctgccag ctgtcacagg aacgatggcc tctttgtcag ggcactgagt gcagttatct
      601 gtgcgcgtta ctctaactgg cactcgaacg tcggcgacgg ggttaccggt ggatcagact
      661 gttcctgtgg cgtaaacgtt tttatgttag taccaacaga tttaccacat ttatcatctt
      721 aacagtttga gtgaaaatgc aggctgcgcc gcaaagggag gagactgagt ggcgtgttca
      781 gtctaaaaga gagttgatgc cagcatacag gggcgaagct ggtcaacaag tcaacattaa
      841 gataatggaa tattcgtaga gaaacgtcag gcagttggcc tcaaatgaac aggaagaata
      901 tatccccagg aaaattaatg ttggtgttat aaacacacca acattaatcc gatctgatta
      961 ttaatcagac aaatatcgtt aatccgtcga attttgaatc aacgtttttt cttacgaccc
     1021 tgaacggcct taaaacgtgg attagattta caaatcacat atagccgtcc ttttcgcttc
     1081 acaatctgac agtctggatg gcgttctttt gcggtacgca gagagttaag aactttcatc
     1141 acgccccctt tttcgtgcta acaaaacgac caaaacgttg ggtgaatcgt gcaacatttc
     1201 cttctgatgc cactgttctc agcttccctg tatagaacgg gtgcgattta gaagagacat
     1261 caattgtcac gtacggatac gttacgccat ccagctcaat ctcacggtct gttttgatag
     1321 tcgagccgat tttaaagtac tcatcaacac tggtgtcgtg gaacaccaca gtacgatact
     1381 caggatggat attgggcttc atcattttta cctgttatgt tatagcataa ccataaagat
     1441 acatgctcat ccggatcatt tcaacaccca atccctgtga ccattagtgg tatgtggaat
     1501 ttccgcaacg gggaagtctg gcattgttaa cagccgttgg cgacgctccc ataaacgcct
     1561 ggatgagtga ttgatgatgg tttcgctgag agtatggatt tttcgacaat ggaggttatc
     1621 tccactttac tggccgatga tttatcgcag tatgattgat tacctgacag cgtgtattcc
     1681 tctcccacgg atataataat tacttacaaa aagggagagg atgcatattt taaatatcac
     1741 tgaagtgaac agtttattgc cgttattaat agaaatggag aaataaatag gcgtattcta
     1801 caattgcgac aaaaacaacg atattaatta gtttatgact gatttattgt actttattct
     1861 ctttcattgg tacttcctcg ctttaaaaa
//
LOCUS       DAJTMK010000338         1838 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTMK010000338 DAJTMK010000000
VERSION     DAJTMK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.345"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..364
                     /locus_tag="OO135_004255"
     CDS             <1..364
                     /locus_tag="OO135_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5399827.1"
                     /translation="GNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRDVT
                     EEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGLVD
                     ALEDDDDVQKVYHNVANL"
     gene            complement(815..890)
                     /locus_tag="OO135_004256"
     tRNA            complement(815..890)
                     /locus_tag="OO135_004256"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(855..857),aa:Asn,seq:gtt)
     gene            complement(991..1838)
                     /gene="yeeO"
                     /locus_tag="OO135_004257"
                     /pseudo
     CDS             complement(991..1838)
                     /gene="yeeO"
                     /locus_tag="OO135_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
BASE COUNT          582 a          433 c          361 g          462 t
ORIGIN      
        1 cggcaatatc ggagcggcag gttctgtcag ctatatgttt gacaatacgg gtgtgattgt
       61 atttaaagga acagaccctg accatatttt tgaaatttta cttgaagctg aagttgatgt
      121 tcgtgatgtg actgaagaag aaggtaacat tgttatttat actgaaccta ctgaccttca
      181 taaaggaatc gcggctctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga
      241 aatgattgct caatctgaag ttgagctttc cccagaagat ttagaaatct ttgaagggct
      301 tgttgatgcc cttgaagatg acgacgatgt tcaaaaagtt tatcataacg tcgcaaatct
      361 ctaattatat tttaaagaaa tctgtcttta tggcagattt ctttaatctc atataatcat
      421 tataaaaata taatgttcaa ctcgtcatat tgcttataaa aaatccccgt tcccagagaa
      481 ataatattta ttaaaattct aggtcttctt tttctgatta ccgaagacaa agtggcaatt
      541 acgtatagtt tcccgataaa gacgcgacag cgacatcccg cataaggcat tgttctcttt
      601 atctttgtac ggtacttcat ggaacagaga ttttgacctt gcgaatcgtg atgtctgttg
      661 ggggcaattt gctcactgaa gcgtgagact cgattaaacg cacgaaacac agaaatcaaa
      721 aaaaccggtc acttttttac aaggtaaccg ggtaaaaata atttttattt tttaactgtt
      781 ttgagattca tagagatgtc tcaaaactaa aatttggctc ctctgactgg actcgaacca
      841 gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga atcgtgagaa
      901 cgaagcgaat attagcgatg cccaaccaca atgtcaaagc ctgtttttta aatttgaaat
      961 cgtttgctga aataatctgc attttgtcgt ttattccgac acagctggct ttttttcaca
     1021 cttttgcggt tcgggtcgag ggtatttcca tagccaacgt ccagtaacca ttcgccagta
     1081 aaacagcacg gcccgcacag cccagtcggc aaacattccc atccagacac caaccacacc
     1141 ccagccaagc atgatgccca gcacataacc gacgacaacc cggcaacccc acatgctcaa
     1201 catagaaacc cacatagcgt aacgggcgtc acgagccccc ttaaatccag cgggcagcac
     1261 ccatgaggcg gaccaaatag gcataaataa agcatttagc caaatcagaa tcacaacgac
     1321 atgtttaacc tgtggatcct gggtgtaaaa cgatgccata accccggcaa agggagccgt
     1381 tagccaggcg atggccgtta atccaagagt ggaaagccag aacacatgcc gcaactgaat
     1441 ctctgcttgc gctatctgtc ctacacccaa ccttcggcct gtaatgatcg tagaagcaga
     1501 gccgagcgca cttccgggta agttgataag agccgcaatt gaaaacgcga taaaatttcc
     1561 ggcaataaca ctggtcccca tcccggcaac gaacatttga gttaataacc gaccactggt
     1621 aaataacact gattcgacac tcgcgggaat accaatcccc atgacttccc agataatgct
     1681 aaaattcagc ggtttaaaat agctctttaa cgaaatcctt agcgcaggat taaaaccaat
     1741 cgccaacacc cacaaaattg caactgcgcc aatataacga gaaatggtta aacccaaccc
     1801 agccccgaca aatcccagtc acggccagga gaaaaggc
//
LOCUS       DAJTMK010000339         1825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTMK010000339 DAJTMK010000000
VERSION     DAJTMK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.346"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>1825
                     /gene="ehaG"
                     /locus_tag="OO135_004258"
     CDS             <1..>1825
                     /gene="ehaG"
                     /locus_tag="OO135_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR5399828.1"
                     /translation="TATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIG
                     ETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISD
                     SSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDT
                     TLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKY
                     IADALDGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYN
                     ASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQ
                     ENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIA
                     LGSSSVSSRVIAKGSRDTIITENGIVIGYDTTDGELLGALSIGDDGKYRQIINVADGS
                     EAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIG
                     IGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVG
                     EFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNT"
BASE COUNT          478 a          459 c          476 g          412 t
ORIGIN      
        1 cactgcaacc agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat
       61 cgccacctac ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac
      121 ctacaaaatc ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc
      181 ctcatttagc acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag
      241 tgccaaacac ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat
      301 ttcagactcc agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt
      361 tgttgatgcg ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac
      421 gtacaccatt gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac
      481 cactcttgac gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc
      541 cgctcacgga aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc
      601 tgctgccagc agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc
      661 tgatgcgctg gatggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta
      721 caccattgcg aacgccgagt acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa
      781 cgccctgctg tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga
      841 cggtaaagcc agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga
      901 tgcagtgaac ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat
      961 tatcaatcag ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat
     1021 taactatgtg cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt
     1081 tggcgctaca gctataggtt ataattctgt cgccaaaggc gatagcagcg tagctattgg
     1141 tcagggcagc tacagcgacg ttgatacggg tatcgcccta ggtagcagct ctgtttccag
     1201 ccgagtgatt gccaaaggct cccgtgacac catcataacg gaaaatggca ttgttattgg
     1261 ttacgacacc acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata
     1321 tcgtcaaatc atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca
     1381 attgcagaat gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc
     1441 aacggaagaa gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat
     1501 cgtgaatggc gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact
     1561 taacggcatt gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg
     1621 taatggttct acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc
     1681 accgcagaac tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac
     1741 aaacgtcgca gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac
     1801 tgatgagcgc gtagcgcaaa atacc
//
LOCUS       DAJTMK010000340         1819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTMK010000340 DAJTMK010000000
VERSION     DAJTMK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.347"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..165)
                     /locus_tag="OO135_004259"
     CDS             complement(<1..165)
                     /locus_tag="OO135_004259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399829.1"
                     /translation="MIFFDEEEAEVLRFDNFDDAVECCADLNIPFFVDAGNKKLVFWF
                     VRVDDEGYPEI"
     gene            complement(1002..1475)
                     /locus_tag="OO135_004260"
                     /pseudo
     CDS             complement(1002..1475)
                     /locus_tag="OO135_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1403..1769)
                     /locus_tag="OO135_004261"
                     /pseudo
     CDS             complement(1403..1769)
                     /locus_tag="OO135_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          416 a          510 c          429 g          464 t
ORIGIN      
        1 gatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagct ttttgtttcc
       61 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt
      121 atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa atcatcttcg tgaagtttta
      181 cgacatagcg gaggagtttg cttctttgag gtgcaggttt tcgtttgcgt ggtttagctg
      241 aacgggccat ataactacct gaaagacaat gatattgtct gtttttataa cggtaatcac
      301 aggccatgac aagccgcagc cgtcaggccg cctactcggg gcggtttaga acgcttattt
      361 agtaccagat cttacttata ataaaggggc ttggcagttt ggataaacta ttgtccttat
      421 tccactaata caaacttttt gtaaagtata ttaaatatac ttcatttcat actgaaatcc
      481 ccggaagtat tcaatcaaac aattacacca taaaaggaag aggggcccca attaagttgt
      541 caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca ttggtactct
      601 ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca ggtccacgtt
      661 ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc gcaggcagta
      721 aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg ctttcgacac
      781 atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc tcacgtccga
      841 ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg atggtttggt
      901 gcctgcgcct gcacgccgac accgaggggc cttccctcat ctcttgtgcc gcatggctgc
      961 acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc atagcaacag
     1021 taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg gcctcgctgg
     1081 atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta gcgtttattc
     1141 agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat cacaccgccc
     1201 tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg ttttccacag
     1261 cagtctcata aaacgtgcat cactgatcct gcggcagacg gctttcatca gcagccggtg
     1321 atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc ccgtgtttcc
     1381 ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg ttcgggccac
     1441 tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt gcacggtaac
     1501 tgatcgggct gaacttccgg gaggcttttg cgctcagttc ctgacggcgc agactggctg
     1561 ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca ggcgtggggc
     1621 accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg ctgacggaac
     1681 tgctgacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc gctgcgggcc
     1741 acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg cggcaacggc
     1801 tttccggctg acggtagtg
//
LOCUS       DAJTMK010000341         1818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTMK010000341 DAJTMK010000000
VERSION     DAJTMK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.348"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(317..1465)
                     /locus_tag="OO135_004262"
     CDS             complement(317..1465)
                     /locus_tag="OO135_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5399830.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          573 a          464 c          396 g          385 t
ORIGIN      
        1 tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa agacatcagc
       61 gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga caaatccttc
      121 gccaaaatta taggggcatc tgtaaaacga gggtttatgc gcggcaaata agaagtgcgg
      181 aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg aataaaattt
      241 ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag taagttcaca
      301 ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg gcgttgcgca
      361 gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat aaattatcgg
      421 agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc acagtatagc
      481 tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc tcgcaagccc
      541 atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat aacgctgggg
      601 ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaag acctgaacac
      661 gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcgaccat tgtccaagga
      721 tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc acccctttgt
      781 gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct gagataatgc
      841 agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt gacgtcgaat
      901 catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag atccctgcca
      961 acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact ttgctgacca
     1021 gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca agcaaacgcc
     1081 cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc caaatcatgg
     1141 cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca atcatcaggc
     1201 caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg cggttaaagc
     1261 caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg gtaaaatagg
     1321 atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc caaagcggca
     1381 cgagtgtatt taacaccgca atcgccagag tcaacaaaag caggccagac agcaaaagca
     1441 tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa atttcatgcg
     1501 catcatgcca ccgccaaaaa cattgtcaat gggtgaaaat acgcgatatc ccagcggcgg
     1561 tattatcgat ttatattact cattcataac gctaatgctg aagagatgat tagtctcatc
     1621 tgattgtttt tattgaacta acacaaattt catacgcttc gttcagtcga aaaatttcat
     1681 ggtcacagca aactcatatt tcccgtctat gctttcagag caatgcatcg cgtctgatcg
     1741 atttcagacg taccactgac tctcgaaagg agaagaggat gaccaaaccg tatgttcgtc
     1801 ttgataaaaa tgatactg
//
LOCUS       DAJTMK010000342         1814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTMK010000342 DAJTMK010000000
VERSION     DAJTMK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.349"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..334)
                     /locus_tag="OO135_004263"
     CDS             complement(<1..334)
                     /locus_tag="OO135_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HCR5399831.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTG"
     gene            complement(437..1393)
                     /locus_tag="OO135_004264"
     CDS             complement(437..1393)
                     /locus_tag="OO135_004264"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5399832.1"
                     /translation="MFSALDIKTLMQETLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNTKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGRDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(1412..>1814)
                     /locus_tag="OO135_004265"
     CDS             complement(1412..>1814)
                     /locus_tag="OO135_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5399833.1"
                     /translation="WEFGIEFFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENA
                     GNLYLRTRFGHGKTENADSLQYNANRTYFKTIRKATLGYEQNIGDDWIAKIEYKHAWE
                     KEHKNFYDPRDEEKYNVLNQDNIYVHAFYRF"
BASE COUNT          529 a          376 c          309 g          600 t
ORIGIN      
        1 gacctgttac agtgaactgc ggattccagt tcatagtcgc aaattgcatt gttaccgcac
       61 ctgctttttc agaataaata agctgataaa taccatctcc cttataatgc attttacgct
      121 ctggcttcat taaccaatag ccttccggga gggttccgga aatgtaaaga cccgcagcaa
      181 ttggccctct ttcatttaca tcagggaggt tacattctga atagcgagga tccgctttca
      241 cgacaccttc ctgactgcca gagggtgaat tgccatcaac gcatcctgaa agaagaccag
      301 tcaacacagc cgcaataagc aatttatttt tcatttaaac tctcaataaa agtagtattg
      361 cttagttaag ctatttctat gtcagtgtta atgttaatgt taatgttaat gtatataaat
      421 aatacttctc aacgactcac tgtttatata caccatgctg tttagttagt ttattaagga
      481 cagcgagatt tttgttgtat tctcgcgtat ttttggctgg gtaaccaaaa aggtcactct
      541 tttcaggaca agaatggctt acacctgatt tagccttaat taccgaataa ctaccaattg
      601 atatgtgacc ggcgattccg acttgaccat gtgtaataac atggtcgcct aaaactacgt
      661 gtcctgaaaa accgcattgt gaaacaatca ggcatttatt accgattatg cagtcacgtc
      721 caatctgcac ttgattatca atacgagtgc cttggcctat aatcgtatct ccaagtgtcc
      781 ctcggtcaat ggtgttgtta caaccgattt caacatcatc acctatgata actctaccga
      841 tgctatcaac tcggacatat gaatcacgtt catccgacat atattcaaaa ctataattac
      901 cgatggagtt attagaatct atgataacgt cattgccaat aatagtacct tctttaatca
      961 ctgtattcgc atgaatggca acattatcac ctatggtcac acagttcata attttaacac
     1021 caggcataaa atggcagttc atacctatct ggcagtgttt accaatgtat acatcaggta
     1081 tcgttgaagt gttttcttgc tcaaacaaac gatacttatg taccttaaaa tatcttaaaa
     1141 ctttgttaag attattcacg ttcaggtaat cgactacaat ctttgcctta gtattagaac
     1201 taatgatctc ttccggtccg ataattatat ccgcaccagt cgttggtata tgcaacaaat
     1261 ctccaggtgt cataacaatc gagagtccac caactaaagg actatggatt ggccgaatgg
     1321 tatcaaccct gagtgatgga tcaccatata aagtttcttg catcaacgtt ttaatatcta
     1381 gtgcgctgaa cataggtttc cctctgttta atcaaaagcg atagaaagca tgaacataaa
     1441 tattatcctg attgagcaca ttatattttt cttcatctct tggatcataa aagtttttgt
     1501 gctctttttc ccaggcatgt ttatattcaa tcttggctat ccagtcgtca cctatgtttt
     1561 gttcataacc tagagtggct tttcgaattg ttttgaaata ggttctgttt gcattgtatt
     1621 gcaaactatc tgcgttttct gttttcccgt gtccgaagcg ggtgcgtaga tacagattac
     1681 ctgcgttttc aaaagcatat ttgataattg gctcgtagta agtttcggta taaatactac
     1741 gatcatttat tttaccgtga ttaaattcat cgttatgctt tttctggtag aaaaattcaa
     1801 taccaaactc ccat
//
LOCUS       DAJTMK010000343         1744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTMK010000343 DAJTMK010000000
VERSION     DAJTMK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.350"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(422..856)
                     /locus_tag="OO135_004266"
     CDS             complement(422..856)
                     /locus_tag="OO135_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5399834.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(844..1245)
                     /locus_tag="OO135_004267"
     CDS             complement(844..1245)
                     /locus_tag="OO135_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399835.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1505..>1744)
                     /locus_tag="OO135_004268"
     CDS             complement(1505..>1744)
                     /locus_tag="OO135_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5399836.1"
                     /translation="DYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARN
                     DDTGFQQVLERADYLAKEHTKAHCTGERNFGCSRS"
BASE COUNT          402 a          499 c          434 g          409 t
ORIGIN      
        1 gctgtttaaa ttcgggagga taattagggc agccttttcg cctgccggga gtcacatttt
       61 tctgcatatc tgacactttg gttcccacta cttatttggt ggacatcact ttgtctaatt
      121 cgtcagattc tgaccagacg gttcaggctg tacgcttacc actattttat ccctgttgaa
      181 tttggttaag ccggtgcaga cgcgccggga aacgcacacc tgaaccggca caataaaata
      241 caatacaacc ccctgttact ggagatacga atggaatgac gaggcagcct gtcagtcaca
      301 gggggctgtt ggcataaaat caacggaaag gccaccaaca ccggaattaa gctccacagt
      361 ccccgcactg gcaatccatt cccgggaaag gcatcaccgc aaactgctcc ggaggcaacc
      421 attacagcct ctgtaacgga ttatcagccc tggcatcttc cgacggcacc tcctgccgcc
      481 cccacgccct gctgtatacc ccggtaccgc agcgtgggca gcacttttcc ccctcataat
      541 cttcgtcaca catcacacaa aaccactttc tcaccttcac cgtataatcc ggaaccgtca
      601 tgccctgcag atgcaaacga cgggcctctt tctctgccgg gtcaagccag gtacatcgca
      661 gcaggatttt ttcagcgaca gtcatctggt catgttcaaa ccacggtggt tgtccgtcat
      721 tgccccgctg aagtgttaac gggcatccac aggacacaca gcacaaaacg tcctgtcggt
      781 tgtgcttcgc agcttctttc gccgtaacaa aatggccttt gctgtcattc gccatataac
      841 agttcagaat tcgcatattt cctcctgagt tgtccgggga atactgaatc ctccggtctg
      901 gcagtgtgtg cagtactgct ccccataata atcgtggtga cattgtctgc agtgccagct
      961 ggctttacgc accacgggta aggcatccgg tacgaattgc tgcagacgtt taatcaactg
     1021 tatttctctg cgctccggcc tgacataagg gcactgttga ccgtgctctg tcagcctgtc
     1081 gtcagtgtgt tcaaaccagg gaagttcagt ttcgtattgc ggatggtatc tgagtgcact
     1141 gccacacaag tggcaggtgt agttagcata aggtgcggcc tgtgcagtac gggcgcccgt
     1201 cagacgtccg ttgccatcaa gagcgataaa ggattttgcg tacatagtca tatacctcag
     1261 ccgccagacg acacgcaggc aacagccgtc ccggtacggg cagcgtggtc agggtgtgaa
     1321 tggcggttca gttaaaatct ggactggagt ttgggtaaag caggctggtt gctgttacag
     1381 cactgtggtt gtcacacaat ggaacactga cgtaaaaaac agtcagtggg cgagtggata
     1441 catcgttgcc gccagacaac aacccatact tttggtttgc cggatattgg cgcgccagtc
     1501 gtgctcaact ccggctacag ccaaaattgc gctcaccggt gcagtgagct ttggtatgct
     1561 ccttcgccag atagtcagca cgttccagca cttgctgaaa gccggtgtca tcattacgcg
     1621 ccagccacac cgccggccag gccggaaaat gcaccagcgt ggcatggcac ccgacatcca
     1681 ctcccagggc actgcaccac gcctgtttaa tcatcccggc cagagactcc ggagcgcggt
     1741 aatc
//
LOCUS       DAJTMK010000344         1735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTMK010000344 DAJTMK010000000
VERSION     DAJTMK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.351"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="OO135_004269"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="OO135_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5399837.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
     gene            1510..>1735
                     /locus_tag="OO135_004270"
     CDS             1510..>1735
                     /locus_tag="OO135_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR5399838.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCA"
BASE COUNT          490 a          292 c          415 g          538 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ctagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg aatagcttgc ttgcaccgta ttgttcttgt ttttcggtta
      961 gttaaacatc gtacttcata tttgaacgtt ctgccggaat gcattatcaa tagaggtaaa
     1021 gtcgcaaccc caaatcgtaa aggaaaccgt agcacgtcgt atgcaagaac gtgccacggc
     1081 tggctgatgg atgttcgata gcgcgagttt gaatgaaaat cagccggaga tgattttaca
     1141 taattgctac ggaattattc aatacaggaa ttgcttgcgt ctgcatggat tgacctgaaa
     1201 ttttcccgaa aatttctcta aaaaactcga aaaaaatggt aactggttga atgtattaat
     1261 atgcaatggt acgtgtcagg gattaaaaga tgaacgtaaa tttattcaac gcattaattt
     1321 taaagggttt tattgtttat tgacgaaaac aggaatcgtg ttcggtctct tttttatctg
     1381 ttaaaagcca gaagcatttc cttcgctgac tttatagtca accataacac acactctact
     1441 gtctgagtcc agcgtttttt aacattcttg ttaagattat gtgatcttta gcgcgggagg
     1501 aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga
     1561 catatctggc tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg
     1621 cattgtcgtt ttgatccgtg gcgagattta cttatctcag tgggagacgt tatcgatcgt
     1681 gggccgcaaa gtttacgttg tctgcagtta ctggaacaac attgggtttg tgcgg
//
LOCUS       DAJTMK010000345         1733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTMK010000345 DAJTMK010000000
VERSION     DAJTMK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.352"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="OO135_004271"
     CDS             complement(<1..666)
                     /gene="ipaH9.8"
                     /locus_tag="OO135_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5399839.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQ"
     gene            complement(928..1023)
                     /locus_tag="OO135_004272"
                     /pseudo
     CDS             complement(928..1023)
                     /locus_tag="OO135_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1059..1364)
                     /gene="ccdB"
                     /locus_tag="OO135_004273"
     CDS             complement(1059..1364)
                     /gene="ccdB"
                     /locus_tag="OO135_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5399840.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1366..1584)
                     /gene="ccdA"
                     /locus_tag="OO135_004274"
     CDS             complement(1366..1584)
                     /gene="ccdA"
                     /locus_tag="OO135_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5399841.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          485 a          355 c          374 g          519 t
ORIGIN      
        1 ttgcagagca tgggatgata atgggttatc actaatatgt attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttcta tcaaaaaaat actctctgac
      121 aacgggatta tttccctcag aaaatgctgg tagctcagtg aggaaattac gggtcgctct
      181 gagattttta agagcctgtg ggagcgatgg taaggagact atttcgttat agctgatatt
      241 catagtcaat aacgaatcgg gtaaagctgg caggttttcc agctcattgt gttgtacctg
      301 aagtgactcc agcgcaggag gtagcacggg caattctgat aatttattgc tggcggaata
      361 taattttttt agcgtaacag gcagttcagg taggttagtt aattgattat atgatacatt
      421 gagcagcgtt atctgagctg gtaagttgtc aggtagcgag gacagattta aacggtccag
      481 tcgaagttca tcggaattat tgataagaca ttctttaagt cgggagacag cctcatcacg
      541 ctcttcaccg gggagcgctt gtttttccca tttatcccat gctgaaaagt aatcagcata
      601 tgtaccggaa atagtgttat aaaaagaatt ttggggcaat gaaaagttat tatttatcgg
      661 taacatgatg ggcatccagt taaagccggt ggacaaatgg ctttgttaaa agaaataagc
      721 aggagttttt atgaagttca ttccatgaaa tgaacttcag cactttttta ttataataag
      781 gataaacaaa attggctgta aaaaaacgat actgtttcag ttaaccgtga atggcctgtt
      841 tgcaccgtat tgttcttgtt tttcggttag ttaaacatcg tacttcatat ttgaacggta
      901 agcggctcgt cagaaccgta ttgatattta ctgagaggtc agatcaactt tccagggcaa
      961 cagatcgcgt acccggtttg ccggccagtc ttggatatgc tcaatgacgt aacgcagcca
     1021 ttttgtcaac gccacgattt ttgaccctag cctgacattt atattcccca gaacatcagg
     1081 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     1141 acgaagatcg gcacactggc catatcagtg gtcatcatgc gccagctttc atccccgaca
     1201 tgcaccaccg ggtaaagctc acgggagact ttatctgaca gcaggcgggc actggccagg
     1261 gggatcacca tccgtcgtcc gggcgtgtca ataatgtcac tctgtacatc cacaaacaga
     1321 cgataacgac tctctctttt ataggcgtaa accttaaact gcatttcacc agtccctgtt
     1381 ctcatcggca aaagagccgt tcatttcaat aaaacgggcg atctcagcca tcccctgctg
     1441 attttcagcc tgccagcgtt ccgcacgcag acggcgggcc tctttttgca tggccgtatt
     1501 taccaggcct gagatattca cgtttgcgga tttaagtaac tgatagctgt cactgtctat
     1561 agtgacagta atacgctgct tcatgataag ccctcaaaaa catacttatg atatgtatat
     1621 gagtatatat taatggtaca aaccatcaat atccataaaa agtacaccat taattcacta
     1681 taagttttta atacaatttt ttctgctgcg atttttttat acgataatta gca
//
LOCUS       DAJTMK010000346         1722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTMK010000346 DAJTMK010000000
VERSION     DAJTMK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.353"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            53..793
                     /locus_tag="OO135_004275"
     CDS             53..793
                     /locus_tag="OO135_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5399842.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     IRLENKGNNPLLVQSWLDTGDDNAEPGNITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGAAIEGNASL"
     gene            828..1722
                     /locus_tag="OO135_004276"
                     /pseudo
     CDS             828..1722
                     /locus_tag="OO135_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          497 a          370 c          364 g          491 t
ORIGIN      
        1 tttctatttt tatattattc cctgttttaa ttaactctgt cagggatggt ttatgttttt
       61 taacaccaaa catacaacag ctttatgctt cgtaacctgt atggctttta gttcatcatc
      121 tattgcggac attgtcattt cgggtactcg cgtaatatat aaaagcgatc aaaaaagtgt
      181 caacatacgt ctggaaaata aagggaataa cccgttgctt gtccagagtt ggttagatac
      241 tggcgatgac aacgctgagc ctggcaatat tacagtccct tttactgcca cgccgccagt
      301 atcgcgtatt gatgccaaac gtgggcaaac aatcaaatta atgtacacag ccagcacctc
      361 actgcctaaa gacagagaga gcgtgttctg gtttaacgta ctggaagttc caccaaaacc
      421 agatgcagaa aaggtcgcga atcaaagtct gctgcaactg gcatttcgca cacgtataaa
      481 acttttctat cgcccggatg gattgaaggg aaatccctct gaagccccgt tagccctgaa
      541 gtggttctgg tcaggttcag aaggtaaggc gtcattgcga gtgaccaatc caacccctta
      601 ctacgtctct tttagcagtg gtgatttaga agctagcggt aaacgctatc cgattgatgt
      661 gaaaatgatt gcaccattta gtgatgaggt catgaaagtc actggcctta atggcaaagc
      721 aagttctgca aaagtacatt tttacgccat taatgatttt ggtgccgcca ttgaaggtaa
      781 tgccagtctg taatcaggca aggatataat tccgcaggaa gcataacgtg actataaaac
      841 caactaaaaa ttatcataat catctgaccc gtatcgccac gttttgcgcg ctgctgtatt
      901 gcaatagtgc tttctgtgct gaacttgttg aatatgacca taccttcctg atggggcaga
      961 atgcctcaaa tattgatctc agccggtata gtgaaggtaa ccctgctata ccgggtatgt
     1021 acgacgtcag tgtttatgtc aacgatcaac ctattattaa ccaaagcatt acatttattg
     1081 aaattgaagg aaaaaaaacg cacaagcctg tatcacactt aagaatttat tgcagtttca
     1141 tataaattct cccgatataa ataacgaaaa agccgttctg cttgccaggg atgaaacact
     1201 cggcaattgc cttaatttga cggaaattat ccctcaggct tctgttcgtt atgacgttaa
     1261 cgagcaacgt ctggatatag acgttcctca ggcatgggta atgaaaaatt accaaaacta
     1321 tgttgatcca tcgttatggg aaaatggcat taatgcggcc atgttgtcat acaacctcaa
     1381 cggatatcat agtgaaactc ctggtcgaag aaatgacagc atttatgctg cttttaacgg
     1441 tgggatgaat ttaggtgcat ggcgactgcg tgcctcaggc aactacaact ggatgaccga
     1501 ttctggcagt aattatgatt ttaagaatcg gtatatacag cgtgatatcg cctcgctgcg
     1561 ttctcaactc attcttggtg agtcttatac aacgggcgaa acctttgatt ctgtcagtat
     1621 ccgcggcatt cgtttataca gcgacagccg catgttgcct ccgacattag cgagttttgc
     1681 gcctatcatt catggcgttg ctaataccaa cgccaaagtc ac
//
LOCUS       DAJTMK010000347         1678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTMK010000347 DAJTMK010000000
VERSION     DAJTMK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.354"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..347)
                     /locus_tag="OO135_004277"
     CDS             complement(<1..347)
                     /locus_tag="OO135_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR5399843.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEG"
     gene            complement(368..994)
                     /locus_tag="OO135_004278"
     CDS             complement(368..994)
                     /locus_tag="OO135_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5399844.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(1450..1632)
                     /gene="fumD"
                     /locus_tag="OO135_004279"
     CDS             complement(1450..1632)
                     /gene="fumD"
                     /locus_tag="OO135_004279"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5399845.1"
                     /translation="MSWSHYRVVGKVVLEMRDLGQEPKHIVIAGVLRTALANKRIQRS
                     ELDKQAMETVINALVK"
BASE COUNT          476 a          384 c          331 g          487 t
ORIGIN      
        1 ttcccttcga taataatgac gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca
       61 cccatatggg catcgacaac taaatttcct ttggtaaaag tagaaagtga ggtgatattt
      121 acgcgagaac tacagggggc accagatcca gttaatgggc cggtagcaaa gactaattta
      181 ttcgcttcat cgaaaggttt cgtgcctggc ggtacttcgt catacataat tttgtagccg
      241 aagcccatgc caccgacaaa acttttaaac ttactggaat cttcgagggt aatatttcct
      301 gtcgtgagat tgactcttaa tatattacct gtccaaccgt tagccatgat tttttccttt
      361 gcaagattca cacagtaata tctttccact cgataatttt taacgcccct gttgggcagg
      421 cgtttgcaca ttcaccgcat aacacacatt tcgaggattt tttactttcg gtatttacgg
      481 tggccatcat ccacgggcat gccgtagtac aggcgctaca gccaatacag cgtttatgat
      541 cgacggtaat acagccttct ttctgctgcc aggtaatcgc accaatcggg cagacgttca
      601 tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt atagttgaga tcgccataca
      661 ggccgccgcc agagccaacc ccgttgtcgc caaagaaata attgcgatgg attttgatac
      721 gggagaagaa tgtccctact gagccatcgt tgaagttggt acaagagatt tcacagcggt
      781 ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc tttgggtgta tttatcaacc
      841 ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg
      901 caattgttag accagcaagg ccttttcctg atatacgcaa gaattctaat cgcgttaagc
      961 caatatctaa tagtggacga tcaaccgggt tcatttattt cgcatcctct tcgcaaacac
     1021 cgtgctgaca attcagcagc ctgatattaa gcattcaact aatacgctgc tgactctgtc
     1081 cccaacacca gatattttcc ctaacccgat agtgtatgag aaaagattta acgccgataa
     1141 actaatcctt tatggttagg agaaaaaata atgtgattat tctgagccct taacattgat
     1201 cgttatcaat taaaattaca aagcaagaaa ttataagtga actgatattt attatcattt
     1261 gaaataaatt taacttaagc gacaattatt tgtgacgttc attgcagaaa ataaaaataa
     1321 tattatcgtt ataaattaaa atacataccg caattcgtat aatttcagca gttgttattt
     1381 agaatgatta actggaccgc aactgaagca agaatatcag gatgaagctt accgcctcat
     1441 cctgaatggt cattttacca gtgcgttaat gacggtttcc atcgcctgct tatccagctc
     1501 actgcgctga atgcgtttat tggctaatgc tgtacgtaat acgcctgcga taacaatatg
     1561 ctttggttcc tgccccagat cgcgcatttc cagcacaact ttaccgacaa ctcggtaatg
     1621 actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgca
//
LOCUS       DAJTMK010000348         1664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTMK010000348 DAJTMK010000000
VERSION     DAJTMK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.355"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(32..214)
                     /locus_tag="OO135_004280"
     CDS             complement(32..214)
                     /locus_tag="OO135_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399846.1"
                     /translation="MYNCGESYSEYDISLSDDENYNDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
     gene            545..1417
                     /locus_tag="OO135_004281"
     CDS             545..1417
                     /locus_tag="OO135_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5399847.1"
                     /translation="MNPSDAIEAIEKPLSALPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMIITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
BASE COUNT          406 a          427 c          390 g          441 t
ORIGIN      
        1 attgattgtc ttattatcag ctaccctcgt attaatcaga gttttcattt atttttagtg
       61 cataatcaga tgccgcctca tcattatcct cagtattatc atagccgaaa acttctcccg
      121 ggcacgggcg ttcacgataa tagtcatcgt tatagttttc atcgtcagat aatgatatgt
      181 cgtattctga atatgattca ccacaattat acataaaatc atctcctttg ggatggttac
      241 aataattagt gagtttattc gaaaaaataa tgagcgccag accaatccac ccgacttttg
      301 attataatca ttaagatgcc tgtctcagta caaatagaat caaactcaat acagcatgat
      361 tcacctttat attctctcag ggaaaccaaa taccactatt caactattaa gaataaaatt
      421 ctggaggtgc gattatctga agatacctgc atcctaaata atttcagaca gatattatcg
      481 ttgtgaatcc cccgccacct ttccggtgcg cgggattttg tcttttttca ccgggaatac
      541 atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctttccg ccctgcctta
      601 ctcactttcc cgtcacatcc tggaacatct gcgcaaactc acccgtcacg aacccgtgat
      661 tggcattatg gggaaaagcg gggccggtaa atcctcactc tgtaatgccc tgtttcaggg
      721 ggaagtcacc ccggtcagtg atgttcacgc cggcacccgg gaagtgcggc gcttccgtct
      781 gagtggccac ggtcacagca tgattatcac tgacctgccc ggggtgggcg agagccggga
      841 cagggatgca gagtatgaag ccctgtaccg tgacattctg cctgaacttg acctggtact
      901 gtggctgatt aaagccgatg accgtgccct gtctgtggat gagtatttct ggcgacacat
      961 cctgcaccgg ggacatcagc aggtgctgtt tgtggtgacg caggccgaca aaacggagcc
     1021 ctgccatgaa tgggatatgg ccggcattca gccttctccc gcacaggcac agaatattcg
     1081 tgagaaaaca gatgcggtat tccgtctgtt ccggcccgta catccggttg ttgccgtatc
     1141 ggcccgcacc ggctgggaac tggatacgct ggtcagtgcg ctcatgacag cgcttcccga
     1201 ccatgccgcc agtcccctga tgacccgcct gcaggacgag ctgcgcacgg aatctgtccg
     1261 gtctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca cggcggagag
     1321 cgtctgtatt gcctctgttg cacgcacggt tctgcgtgcc gtccgtgact cggtggtctc
     1381 tgttgcccgc gctgtatgga actggatttt cttctgaaca gtctgccagg ggaaatgcct
     1441 gattacattg cgttaaccaa ctgaaaacat gacacaaaca cctcatgctc ctcctgtttc
     1501 tcttgcgtga ccgccttgca atcaatagaa taaaacgatc gataaaacag gtatcgatcg
     1561 ttactatcga tcgtttatag tggttaatgg cctgcgaaaa ggcagttgaa tctcttcatc
     1621 atgcagaacg gaattgcaca caacggactg atacttctct ggcg
//
LOCUS       DAJTMK010000349         1624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTMK010000349 DAJTMK010000000
VERSION     DAJTMK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.356"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1064
                     /locus_tag="OO135_004282"
     CDS             <1..1064
                     /locus_tag="OO135_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR5399848.1"
                     /translation="PCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHLPLYRQQ
                     QVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKILNTKKG
                     GKSCSGYQWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHKAYRTLA
                     NKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRPVEKIRQ
                     WRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGAVPLDNN
                     VCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDVLMRLPE
                     WPEERLAELLPLEGFTFSG"
BASE COUNT          387 a          442 c          459 g          336 t
ORIGIN      
        1 gtccctgttg ccagaaggtg ttcagcggtg aaatgccggc acatatcctc ccgaaaagtg
       61 ccgttgagcc atcagtcatc gcacaggtga tcatcaataa atacggtgac cacctgcctc
      121 tgtatcgcca gcaacaggtc tttgcccgtt cagatgtcgg gctgcccgtc agttcgatgg
      181 ctgacatggt tggcgcggcg ggtgccgcat tatctcccct ggcggcgtta ctccatcgcg
      241 agttgataaa ccgtccggtg gtgcatgcag atgagactac cctgaagatc ctgaacacga
      301 agaaaggcgg taaatcctgc tccggttatc agtgggcata cgtcagtgga gaaaggacgg
      361 gaccgtcagt tgtgtgcttc gactgccgga ccggacgtag ccatgagtat cctgaaaact
      421 ggcttcaggg ctggggcggg acgctggttg tcgacggaca taaagcttac cggactctgg
      481 caaacaaagt gccggagatc acgctggccg gatgctgggc ccatgcccgc aggggcttcg
      541 ccgacctgta taaaatcagt aaagatccac gggctgccat agccgtgaag aaaatcgcgg
      601 ggttgtaccg tcttgagaag aagatcagta gccgccccgt ggaaaaaatc cgccagtggc
      661 gacagcgtta tgcccgtccg atactggaag aactgtggtc atggcttgaa gagcaggaac
      721 cgcaatgttc tccgggaaag gcattacaca aagccattgc ctatgcgctg tctcatcgcg
      781 tggaactgag ccgcttcctg gaagatggtg cggtgccgct ggataataat gtgtgtgaac
      841 gggccatcaa aaacgtggtt ctgggcagaa aatcgtggct gttcgccggt tcgcagatgg
      901 cgggagaacg cgccgcgcaa ataatgagct tgctggaaac cgcgaaacgc aacggtctgg
      961 agccgcatgc ctggttgaca gacgtcctga tgcgtctgcc ggagtggccg gaggagcgac
     1021 tggcagagtt gctgcctctt gagggattta ccttctccgg gtgagtgata cctgccgtca
     1081 ggtgttcgtg caccgggcca taacctgcag tcgggagttg aactcctgac ggcaggaaat
     1141 gagccagacc agcaaccacg cgggctgaca gcgaataaga acctgaatca gcccggagtc
     1201 attcccgggt cagtacatca tcacctgcaa ttaatccccg agtcagtact tcgcggcctg
     1261 acgcgatcac gctactcagt acttcagagc ccgcctccgg tacctgcctg gcgatcgcct
     1321 ctgtgcaaca aaataaatca accgttatca aaccacgagc gttcgtatgc gccgccgatg
     1381 acgcgcgcct gaaaaacgcg tcaatatgag ttccccggaa gcttaccagt aaacagagca
     1441 gacgatcggt cttccgataa taaccgaagg ggtcgacagc gccacaatac gggcaacata
     1501 ctgtattatg agtaattgcg ttaccacagt ccctgcactt ctttttgcgt atcacgatcg
     1561 ctccctctcc acgccttatc cgtaaccggt tttttacatt aaaaaaacaa cgccgggaat
     1621 tcaa
//
LOCUS       DAJTMK010000350         1614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTMK010000350 DAJTMK010000000
VERSION     DAJTMK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.357"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..238)
                     /gene="fimC"
                     /locus_tag="OO135_004283"
     CDS             complement(<1..238)
                     /gene="fimC"
                     /locus_tag="OO135_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5399849.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGV"
     gene            complement(275..814)
                     /locus_tag="OO135_004284"
     CDS             complement(275..814)
                     /locus_tag="OO135_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5399850.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(879..1427)
                     /gene="fimA"
                     /locus_tag="OO135_004285"
     CDS             complement(879..1427)
                     /gene="fimA"
                     /locus_tag="OO135_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5399851.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
BASE COUNT          407 a          433 c          347 g          427 t
ORIGIN      
        1 ttacaccatc ggcattttcc atccatgatt gaattaaata ggtactattt tcatcattat
       61 ttgtcacggc aagttgcact tgtttttgcc ctgccggata aattacgcga gtcgcaccta
      121 aggccactcc cgcttcagcg cgtccggcaa ccatcattgc catgaacatc aggatacctg
      181 ccagcaagca gaatgttatt tcctgcgatt tccttacatt ggcgttttta ttactcactg
      241 tcctgttcct gttatcacat tatctgctga acaattactg ataggttaaa gagaaccagg
      301 cctgggcatt ggcgatgccg ccagtaaccc aacgcccggt agcacgatat ttggcgatga
      361 aatgtagcga agtagagcct gagtaaagcc gtttccagtt tactggagga cgattaatcg
      421 gtacgaggtt tccttcatca tcaaacaacg ctacgccaat attggtggct atccctggcc
      481 cctctcccac ggaaagcaca tccggatttt taccatccgc gacaccgtga aacgccacac
      541 ccacacgttc actcaccacc gtgctacatt cccgtaaatg aataacaaaa ggcaccggtg
      601 cgctatcttc ccctaccgca tgaaaccggt tactgctgat ttgccccata ttgaccgtca
      661 tttgtttatc accggcttca atccggcaag tttccgcaat aatgacgccc tgaaattgca
      721 tatttccgcc gggcagcgtg gtattccatt tatttccggc cagagcaaac attggcagca
      781 acgacgctaa tagaaataat ctcttccttt tcatcgttat ttttatcgca caagggtggg
      841 catccctgcc cgtaatgacg tccctgaacc tgggtaggtt attgatactg aaccttgaag
      901 gtcgcatccg cattagctgc gccaggggtt gcgacaccgg ttgcataata acgcgcctgg
      961 aacggaatgg tgttagtacc gttattcagg gttgtttgct cactgaatgt cgcaccatcc
     1021 agcgtcagcg cagcacccgt tctgtccagg atctgcacac caacgtttgt tgcgctaccc
     1081 gcagctgaac tctgcagagc cagaacgttg gtatgacccg catcaatcgc cgtacctaaa
     1141 aaggcaactg cggctttaga tgcaacattg gtatcgcaat cattcagctg aatgttaaaa
     1201 ccgacagcag aactggttgc tccttcctgt gccagcgatg cggtacgaac ctgtcctaac
     1261 tgaacggttt gatcaacaga gcctgcatca actgcgcaag cggcgttaac aacttcccct
     1321 ttaaagtgaa cggtcccacc attaaccgtc gtggcagcgg ccagagccgc tgtagaactg
     1381 agggacagag ccgacagaac aacgattgcc agagttttaa ttttcatgct gctttccttt
     1441 aaaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga
     1501 gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg ggccattttg
     1561 actcatagag gaaagcatcg cgcacaaact ttttcagttt atttgttggc ttaa
//
LOCUS       DAJTMK010000351         1588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTMK010000351 DAJTMK010000000
VERSION     DAJTMK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.358"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..438)
                     /locus_tag="OO135_004286"
     CDS             complement(<1..438)
                     /locus_tag="OO135_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5399852.1"
                     /translation="MKVNKKRLAEIFNVDPRTIERWQSQGLPCASKGSKGIESVFDTA
                     MAIQWYAQRETDIENEKLRKELDDLRAAAESDLQPGTIDYERYRLTKVQADAQELKNA
                     REDGVVLETELFTFILQRVAQEISGILVRVPLTLQRKYPDISPS"
     gene            826..1020
                     /locus_tag="OO135_004287"
     CDS             826..1020
                     /locus_tag="OO135_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5399853.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            complement(1214..>1588)
                     /locus_tag="OO135_004288"
     CDS             complement(1214..>1588)
                     /locus_tag="OO135_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5399854.1"
                     /translation="TYKAQRDKNVRELKLANAAITDMQMRQRDVAALDAKYSRELADA
                     RAENETLRADVAAGRKRLRINATCPGPVREATGTARVDNATGPRLADTVTRDYFTLRE
                     RLMTMQKQLEGAQEYIRTQCLK"
BASE COUNT          425 a          421 c          355 g          387 t
ORIGIN      
        1 tgatggtgaa atgtccggat atttacgctg taatgtcaac ggcacacgca caagtatccc
       61 cgaaatctcc tgtgccacac gttgcagaat gaaggtaaac agttcagttt ccagcactac
      121 tccgtcttca cgggcatttt tcagttcctg cgcatctgcc tgcacttttg tgagccggta
      181 gcgttcatag tcaatggtgc cgggttgtaa atctgactcc gctgccgcac gcaaatcgtc
      241 cagttctttg cggagctttt cgttttcgat atcagtttcc ctctgcgcat accactgaat
      301 tgccatggca gtatcaaata cagattcaat gcccttacta cctttggagg cgcaagggag
      361 tccctgagac tgccagcgtt caatcgtccg cgggtccacg ttgaaaattt cggcaagcct
      421 ctttttatta accttcatga aaacaaccca ttatcaaata caaggcccga catgaaaacg
      481 ccagaaaaag gcattttcgg acactttcat gtcggacctt tacggatgca atattaaaaa
      541 aaacaaaaag ttatattcga gaagtaccga cacgattttc cctgaaaaat tttcataaat
      601 agtgaaaaac cgcgaggtcg ccgccccgta acctgtcgga tcgccggaaa ggacccgcaa
      661 aatgataata attatcatct acatgtcaca acgtgcatct acgccatcaa actacgtcaa
      721 ataatcaatt atgacgcagg tatcgtatta attgatctgc atcaacttaa cgtaaaaaca
      781 acttcagaca atacaaatca gcgacactga atacggggca acctcatgtc aacgaagaac
      841 agaacccgca gaacaacaac ccgcaacatc cgctttccta accaaatgat tgaacaaatt
      901 aacatcgctc ttgagcaaaa agggtccggg aatttctcag cctgggtcat tgaagcctgc
      961 cgtcggagac taacgtcaga aaagagagca tatacatcaa tccaaagtga tgatgaataa
     1021 acatcccggt ttcttccacc atcgcaccgg acaggcgact atgaggggac aacgccgcgc
     1081 tccgttaacg cggtaaaccc cggtgtgtat cgtttttgat tatccccgca cactcgcgca
     1141 gaggagcctc cctgtcgggc tgcggtctct gttaatgagg gaatacagcg acgatacggc
     1201 gcatcaacaa aacttatttc aggcactgag tacggatata ttcctgcgcc ccttccagtt
     1261 gcttctgcat cgtcatcagc cgctctctga gggtgaaata atcccgtgta acggtgtctg
     1321 ccagtcgggg gccggttgca ttatccacgc gggcggtgcc ggtggcttca cgcacgggac
     1381 ctggacaggt ggcgttgata cgcaggcgct tacgaccagc ggcaacgtca gcgcgaagag
     1441 tttcattttc agctctcgca tcggctaatt ccctcgagta ttttgcatcg agcgcagcaa
     1501 catcacgctg acgcatctgc atgtcagtaa ttgccgcgtt cgccagcttc agttctctga
     1561 catttttgtc gcgctgggct ttgtaggt
//
LOCUS       DAJTMK010000352         1587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTMK010000352 DAJTMK010000000
VERSION     DAJTMK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.359"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..194)
                     /locus_tag="OO135_004289"
     CDS             complement(<1..194)
                     /locus_tag="OO135_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399855.1"
                     /translation="MKIEELRENFSENGLYAVRVENGAIVSHCCIRCLQSQQRKSGAV
                     LFYFCNGLLTDDFILREDEFA"
     gene            complement(444..599)
                     /locus_tag="OO135_004290"
     CDS             complement(444..599)
                     /locus_tag="OO135_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5399856.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYKSRK"
     gene            complement(827..1492)
                     /locus_tag="OO135_004291"
     CDS             complement(827..1492)
                     /locus_tag="OO135_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5399857.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          403 a          413 c          353 g          418 t
ORIGIN      
        1 gcaaattcgt cctcacgcaa aataaaatcg tccgtcagaa gtccattaca aaaataaaat
       61 aacacagcac cgctcttcct ttgttgagat tgcaaacatc taatgcagca atggctgaca
      121 atcgctccat tctcaacgcg cacagcatag aggccatttt cactaaaatt ttcacgcaat
      181 tcttcgattt tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca
      241 tccctcttct ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat
      301 caaattcacc ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg
      361 ggcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg
      421 atcagctccc cgcccggtca ctcttacttc ctggatttgt agtctacgaa gacagcgacc
      481 tccgtctggc cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc
      541 actatgacgg ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata
      601 gcctgcttct ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa
      661 ttggcctcag attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct
      721 ctatctgcct tttagtgttc atgcctgaag cagatagcct caagcacccg cagtcattct
      781 acttacctcg ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt
      841 ctttaccgta gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta
      901 gattggtatc gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc
      961 gcttgctgat ttcaatcata cgggacgagc ctccctgctt gcgccagtta taatcccagt
     1021 acaccatgcc cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat
     1081 ttttccagcg tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt
     1141 acagcgctgt ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg
     1201 gctcccattc cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa
     1261 taaacggcac gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact
     1321 ctttctgagg atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct
     1381 gaagcgcctc catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt
     1441 ttgccccgtt tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt
     1501 ttattggggt cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga
     1561 tgtgtgactg ctccccgccc tttcggg
//
LOCUS       DAJTMK010000353         1580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTMK010000353 DAJTMK010000000
VERSION     DAJTMK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.360"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            97..1580
                     /locus_tag="OO135_004292"
                     /pseudo
     CDS             97..1580
                     /locus_tag="OO135_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          550 a          287 c          279 g          464 t
ORIGIN      
        1 gcgatttatg atatttcttt acttgtaatc atattaaatt ttttcactgc tctgatagtt
       61 aaaattcaag acatcaataa acaatgagat atttaaatga ttactcgtat tcctcgtagt
      121 tctttctctg caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa
      181 ttgttttata aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta
      241 aaaagcttct cagagaactg tagacaaaat ggtcgtcata tctttagtaa caaggatttt
      301 gtcattaatt ttccaagtcc gtcttacaag ctgataagaa agaaattacg ataattaata
      361 aaaatgaaaa cacgacactc actcaaacca ttgctccaat atttgaagaa tacctaatgg
      421 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccta aactcagtta
      481 gaaaagaaaa agaattccca agagttaaac ttaatggtca atgttatttt ccggggcgcc
      541 cccaaaaccg tatagtatgc cgacacattg ctgcacaata tattaatgat atttatcaga
      601 atgttgatta caaaccccat aaagatgatt actcttcagc tgaaaaattt ctcacgcact
      661 tcaacaaaaa atgcaaaaac cagactttgg agttggtttc cagccgtcct gaggggcgtt
      721 gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac aagatggaat
      781 caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct ttgacggtcc
      841 ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg gtttatgatc
      901 ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat attaaacact
      961 attctctgat ggtttttatg aatgtagatt atagcctcct gaaatggtca aatgatcata
     1021 ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa
     1081 aaggatctgt ggatgaaata acccctccgt tatcacctgc aacgatgaat ttgctaatgg
     1141 caattggtca gaatcaccaa cttacgcaac tgatgattca actccagaaa atgccagaac
     1201 tacatagaac agaaatgttg actgcctata atagtattaa cctgacaggt ttatatttgg
     1261 ctataaatta tggtaatgct gatatcgttg agactatttt caattcattg tcagaaacag
     1321 gatatgaagg gttactctcg aaaaaaaatc tcatgcatat tctggaggca aaagataaaa
     1381 atggtttttc ttgattattt ttagcgatat cacgtaagga taaaaatgtt gtaacctcga
     1441 ttctgaacgc cttacctaaa ctggccgcaa cacatcattt agataacgaa caagtgtata
     1501 aattcctgag tgccaaaaat agaacgtcca gccatgtttt ataccatgtt atggcgaatg
     1561 gtgatgccga catgggtaat
//
LOCUS       DAJTMK010000354         1580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTMK010000354 DAJTMK010000000
VERSION     DAJTMK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.361"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(9..224)
                     /locus_tag="OO135_004293"
     CDS             complement(9..224)
                     /locus_tag="OO135_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5399858.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(517..591)
                     /locus_tag="OO135_004294"
     tRNA            complement(517..591)
                     /locus_tag="OO135_004294"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(556..558),aa:Gly,seq:tcc)
     gene            complement(595..670)
                     /locus_tag="OO135_004295"
     tRNA            complement(595..670)
                     /locus_tag="OO135_004295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(635..637),aa:Thr,seq:tgt)
     gene            complement(673..749)
                     /locus_tag="OO135_004296"
     tRNA            complement(673..749)
                     /locus_tag="OO135_004296"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Arg,seq:tct)
     gene            complement(751..827)
                     /locus_tag="OO135_004297"
     tRNA            complement(751..827)
                     /locus_tag="OO135_004297"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(791..793),aa:Ile,seq:cat)
     gene            complement(1017..1571)
                     /locus_tag="OO135_004298"
     CDS             complement(1017..1571)
                     /locus_tag="OO135_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5399859.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          426 a          425 c          330 g          399 t
ORIGIN      
        1 tggcgacatt attcacctct cgcagcctta cgcttatctt ctcttatttt gaaatacaga
       61 ttcgtcagat aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac
      121 tgtgatggac tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag
      181 gtgccgtagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
      241 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca
      301 aaagcgggcg aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag
      361 ttaatcggaa taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct
      421 gcacagcaat tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc
      481 gggatttccc atcatcgcag actgaaagac tctgactgga gcgggcagcg ggaatcgaac
      541 ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg catatggtgc
      601 cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgctct gcctgctgag
      661 ctaagtcggc gctggcccac caccgaggac tcgaacctcg caccgtcaac ttagaaggtt
      721 gatgctctat ccggatgagc tagtggtggt tggtggccct tgctggactt gaaccagcga
      781 cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga ggcagaataa
      841 taaccatatg tcatcacatc tgcaaactca tctgaccacc agcacgttta acgtcctgtg
      901 ccgtttttca ggtataaaaa aacccgcata aagcggactc tttcaaatgt ccatgtctgc
      961 tattcgcctc gcggtacagc tttgcgaagt gtacaagaat tgaagcagtt tttacgtcaa
     1021 aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta caaaataaat
     1081 tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact ccatttcggg
     1141 tgtgcatcat tcagcagttc agccattttg cgtttggtca tcccccgccc ctcataccgt
     1201 tgccgaagga cgctaatcaa tcctggatgc tctgccagca cctcacttat gacccgatca
     1261 atacataacg cctctgcatc agtacaatgc gccagccagc tcttttgctt accgttaatc
     1321 atatcccgca aaaaagcctc aagttcagac ttgttcagac ctgctttttt catcctccgg
     1381 agcgcctcgt taattgccgt ttttgtcagc tttttagagg ccaacaactg gttgaacata
     1441 ttccccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag ttttccctga
     1501 atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc tgtatttgtt
     1561 gggtaaatca taaataacct
//
LOCUS       DAJTMK010000355         1554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTMK010000355 DAJTMK010000000
VERSION     DAJTMK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.362"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            203..355
                     /gene="hokA"
                     /locus_tag="OO135_004299"
     CDS             203..355
                     /gene="hokA"
                     /locus_tag="OO135_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5399860.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(534..746)
                     /gene="cspA"
                     /locus_tag="OO135_004300"
     CDS             complement(534..746)
                     /gene="cspA"
                     /locus_tag="OO135_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5399861.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1027..1317)
                     /locus_tag="OO135_004301"
     CDS             complement(1027..1317)
                     /locus_tag="OO135_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5399862.1"
                     /translation="MEYKDPTHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSNQLME"
BASE COUNT          437 a          326 c          332 g          459 t
ORIGIN      
        1 ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc tacgggtgct
       61 tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta
      121 taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg
      181 aaatcaacac agggggctgg gaatgccgca aaaatacgga ttactttcat taatagtgat
      241 ttgtttcacg cttttatttt tcacctggat ggtaagagat tcactgtgtg aattgcatat
      301 taaacagggg cgttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc
      361 ttcggcggga aattattccc gcctttctta cggcgttgcg cattctcatt gcacccacat
      421 ttattcttca caaaaataat aatagatttt actacgcgat cgattattta tttcctgaaa
      481 aaaaataaaa aaaatccccg ccaaatggca gattctgtgc ttttaagcag agattacagg
      541 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      601 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      661 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      721 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      781 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      841 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      901 accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt tttcatattt
      961 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
     1021 aaaagtctat tccatcaact gattacttaa tgccgggttg gcttgaatca aacgcatcaa
     1081 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1141 tgatatgccc aaaacccggg cgaaatcatc gatttttaac cctgtccctt ttcgtaactg
     1201 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1261 aaaaacaatc tgttccaggc tgctcaacag ctcatgcgtt ggatctttat attccattga
     1321 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1381 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1441 agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac
     1501 cactcctgtt ttgtaaagat ttcgaaaatc taaagttctg cgaaggcgta aatc
//
LOCUS       DAJTMK010000356         1525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTMK010000356 DAJTMK010000000
VERSION     DAJTMK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.363"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            141..1525
                     /locus_tag="OO135_004302"
                     /pseudo
     CDS             141..1525
                     /locus_tag="OO135_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          513 a          224 c          266 g          522 t
ORIGIN      
        1 tttatagtac ttcacttctt cttcactaag aatgtccatt atctcaatgc cttatcagag
       61 attcttttcc tttcgccggt agtgtctgga cgctcaggct acttttccag gttattttat
      121 ttctgtatta cagaggtttt atgataggtc atatcacaaa ttctggtggc aataactctt
      181 tgatgaaaca tgatgtggtt caaggaaata atatagtaga tcttgattta ctacgtaatt
      241 tcaatggggt gacaggttta aacagagata actttattta tatcagtgat atatttttaa
      301 atataaaaca acggaacgaa aaaatcatgc aataaatatg ttacgtgaag tctcaattag
      361 taatgatact ataagtgtaa aattctacag aaatgaaaaa atagaatgcg cttgtaattt
      421 tctgatggat aaagatgcgc aggggtatat cgacctgtct gattttgatg taacaaattg
      481 tcattttaaa ggtgacgtta tttctaaggt gtctttttta tcatcaaatc tacaacatgt
      541 aactttcgaa tgtaaagaaa ttgagaattg caattttact aaggcaacag ttgataatgt
      601 catatttaaa tgtcgacgtt tacacaatgt gatttttctc aaaacgagtg gcgaatgtgt
      661 tgattttagc caaaatattc ttgatacagt tgacttctcg cagagtcaac ttggtcatag
      721 taattttcgc gaatgtcaga ttagaaattc aaacttcgat aattgttatc tttacgcttc
      781 gcacttcacc agagcagagt ttctgtctgc caaagaaata tcatttatta aatcgaattt
      841 gacagctgtt atgtttgatc atgtgcgaat gtcgacaggg aattttaaag attgcattac
      901 agaacaatta gaattaacta ttgattattc agatatattt gggaatgaag atctcgatgg
      961 ttatatcaat aacattataa aaatgattga tacattgcca gataatgcaa tgatattgaa
     1021 atccgttctg gcagtaaaac tggttatgca attaaaaatt cttaatattg ttaataaaaa
     1081 ctttattgag aatatgaaga aaacatttag ccattgtcct tatataaaag atccaattat
     1141 acgcagttat atccattctg gtgaagataa caagttcgat gattttatgc gtcaacatcg
     1201 attcagcaag gtggatttcg atacccaaca gatgatccat tttattaaca ggtttaatat
     1261 gaataaaggg ctgattgata aaaataacaa ttttttatcc aacttatcga tcaggcctta
     1321 cgatcaacgg atgatatgat caaagcaaat gcctggtatc tttataaaga gtggattcgt
     1381 agtgatgatg tttcacctat atttatagaa actgaagaaa agttaagaac ctttaacact
     1441 aataaattaa cgcgaaacga taatatattt atcctgttct cctctgtcga tgatgggcct
     1501 gttatggtgg taagctccca gcgct
//
LOCUS       DAJTMK010000357         1516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTMK010000357 DAJTMK010000000
VERSION     DAJTMK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.364"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>1516
                     /locus_tag="OO135_004303"
     CDS             <1..>1516
                     /locus_tag="OO135_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5399863.1"
                     /translation="ARSRGKRAGVAVVLSLAAVTSVPALAADTIVQAGETVNGGTLTN
                     HDNQIVLGTANGMTISSGLEYGPDNEANTGGQWIQNGGIANNTTVTGGGLQRVNAGGS
                     VSDTVISAGGGQSLQGQAVNTTLNGGEQWVHEGGIATGTVINEKGWQTVKSGAMATDT
                     VVNTGAEGGPDAENGDTGQFVRGNAVRTTINENGRQIVAAEGTANTTVVYAGGDQTVH
                     GHALDTTLNGGYQYVHNGGTASGTVVNSDGWQIIKEGGLADFTTVNQKGKLQVNAGGT
                     ATHVTLKQGGALVTSTAATVLGSNRLGNFTVENGKADGVVLESGGRLDVLEGHSAQKT
                     RVDDGGTLAVSAGGKATGVTMTSGGALIADSGATVEGTNASGKFSIDGTSGQASGLLL
                     ENGGSFTVNAGGLASNTTVGHRGTLTLAAGGSLSGRTQLSKGASMVLNGDVVSTGDIV
                     NAGEIRFDNQTTPDAALSRAVAKGDSPVTFHKLTTSNLTGQGGTINMRVRLDGSN"
BASE COUNT          371 a          369 c          487 g          289 t
ORIGIN      
        1 ggcccgctca cggggaaaac gcgccggtgt ggcggttgtg ctgtctcttg ctgctgtcac
       61 atcagtcccg gcactggctg ctgacacgat tgtacaggcg ggagaaaccg tgaacggcgg
      121 aacactgaca aatcatgaca accagattgt tctcggtacg gccaacggaa tgaccatcag
      181 tagcggtctg gaatatgggc cggataacga ggccaatacc ggcgggcaat ggatacaaaa
      241 tggcggtatc gccaacaaca ctactgtcac cggtggtggt cttcagagag tgaatgccgg
      301 aggaagcgtt tcagacacgg ttatcagtgc cggaggcgga cagagccttc aggggcaggc
      361 agtgaatacc actctgaacg gcggtgagca gtgggtacat gaaggcggga ttgcaacggg
      421 taccgtcatt aatgagaagg gctggcagac cgtcaaatcc ggcgcaatgg caaccgacac
      481 ggttgtgaat accggcgcgg aagggggacc agatgcagaa aatggtgata ccgggcagtt
      541 tgttcgcgga aatgccgtac gtaccactat caatgaaaat ggtcgtcaga ttgtggctgc
      601 tgaaggaaca gcaaatacca ctgtggttta tgccggcggc gaccagacgg tacacgggca
      661 tgcgctggat accacactga atggcggtta ccagtatgtg cacaacggcg gtacggcgtc
      721 tggcactgtt gtaaacagtg acggctggca gattatcaag gaaggtggtc tggcggattt
      781 caccaccgtt aaccagaaag gcaaactgca ggtgaacgcc ggtggtaccg ccacacatgt
      841 caccctgaaa cagggtggag cactggttac cagtactgcg gcaacggttc ttggcagcaa
      901 ccgtctgggc aatttcacgg tggaaaacgg taaggctgac ggagttgttc tggagtccgg
      961 cggtcgcctg gatgtactgg aaggccattc agcgcagaaa acacgggtgg atgacggcgg
     1021 aaccctggca gtgtctgccg gtggtaaggc aacaggtgtc accatgacat ccggtggtgc
     1081 cctgattgca gacagtggtg ccactgttga ggggaccaat gccagcggta agttcagtat
     1141 tgatggcaca tccggtcagg ccagcggact gctgctggaa aatggcggca gctttacggt
     1201 taatgccgga ggactggcca gcaacaccac tgtcggacat cgtggaacac tgacgctggc
     1261 tgccggggga agtctgagtg gcagaacaca gctcagtaaa ggtgccagca tggtactgaa
     1321 tggcgatgtg gtcagtaccg gcgatattgt taacgcaggg gagattcgct ttgataatca
     1381 gacgacaccg gatgccgcgc tgagccgtgc tgttgcaaaa ggcgactccc cggtaacgtt
     1441 ccataaactg accaccagta acctcaccgg tcagggtggc accatcaata tgcgtgttcg
     1501 ccttgatggc agcaat
//
LOCUS       DAJTMK010000358         1512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTMK010000358 DAJTMK010000000
VERSION     DAJTMK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.365"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1090..1416
                     /locus_tag="OO135_004304"
     CDS             1090..1416
                     /locus_tag="OO135_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399864.1"
                     /translation="MFKYAEMLMAEEDDKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALDVNSWRGKSKYPDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          453 a          261 c          296 g          502 t
ORIGIN      
        1 ccttattcag tcgaccttca aaataaacgt ttaataggga caatcaccat ttggattgtc
       61 cgctgaggag gcgaacgttt tcctagacag ctttatcatg tcagcccaca ttgttggcgc
      121 tgacaatact aaggacaaac gatggggaag tatcttttta gcgttatttt cgggagtaaa
      181 aaacatggta tctttccgta tgtcagttgt atcttgctgg tattcgtagt cgtgataagg
      241 catacaacga tatttatctt cacgttagtg aagaataagt tgttttgtga tctatgttaa
      301 tctgggatta caatccgggc ttgatacatc gcatcacgac ttttattctc ttcagaatag
      361 aatatgttac tccccgtttt acaggctcag gatttgaatt acaaattata cttttaagtg
      421 aacaataacc aggggtattt cttatttcct gaaaccaggc ttggcccatt tcgggatgag
      481 tttgaaaaaa ctcttgacgc atacgatagt gatgaaataa acgaaacaaa ttacgtcaac
      541 aaactcaggc gtcttgcctg acatgctcct gacatcattg atattcacgc tcatctggca
      601 tatgcatttg cgctctttat gctgcaattc tggcgaatgt tcatttacag cgtcatcagg
      661 atgccgttat actcattgat aaaatactaa catataaccc tgataataat caggggacac
      721 gctggttact ggggacggaa ttgttacgtg ctgcaagcat gacagggctc tgtacgtatt
      781 gacaggacat gtcaatgaat ttccccccta ctggtacgag cttgctcttt tacattttat
      841 gaatgaggca tatattaatg ctgcaacggc atttcgatat agatttttaa caagcatata
      901 ttgttgaaat gctttgttag aatctttatc cattccctgg ggctgtatag tatgattttt
      961 caggtatttc tgaatacagc caaatattat tgcaaaatat tctctaaatt attaattaaa
     1021 tatccataag cattattgcc tgggaactgg acatataatt actcttctgt gctaaaggaa
     1081 cgttctgaaa tgtttaagta tgcagagatg ttgatggcgg aagaagatga taaagtatgt
     1141 gaacttattt ttaaacaaca agataatttg attttaaaaa taaatgatag atttcccaga
     1201 ataattataa agaaataccg aacattacaa ggtaaagtcg tatggctatg gatattgcca
     1261 tttgttattg aatggttaat cttgtgtgct ttagacgtaa atagctggcg aggcaaaagt
     1321 aaataccctg attattattt gttaagagat gagatgcctg tgcgtctagt gtctattata
     1381 ttcccaacat tattggcgct attcttgctg gcgtaggcat cgccatacta tgaatattgt
     1441 gtttgtcgtg attcaatttt tatttataac ctctacccgg agcaaattca ggtaggtcat
     1501 gcataaaaat at
//
LOCUS       DAJTMK010000359         1506 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTMK010000359 DAJTMK010000000
VERSION     DAJTMK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.366"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..469
                     /locus_tag="OO135_004305"
     CDS             <1..469
                     /locus_tag="OO135_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5399865.1"
                     /translation="VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
     gene            697..1056
                     /locus_tag="OO135_004306"
     CDS             697..1056
                     /locus_tag="OO135_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399866.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGDTNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(1049..1495)
                     /locus_tag="OO135_004307"
     CDS             complement(1049..1495)
                     /locus_tag="OO135_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005045045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5399867.1"
                     /translation="MIQLAFGWNDEHLYMFEKGRKGDPGSEYRVWGEDESMGNAAITP
                     LWAALQNEGDKLVYTYDFGDWWDCVIVLEKQTHDTSNQPISCLRGKGTTPAENSGGLH
                     GYNELLLQARESDNPEQAEIHNFLMLDIERRVYDLSRINDRLQAIN"
BASE COUNT          386 a          372 c          370 g          378 t
ORIGIN      
        1 ggtggcatgg ataaacccgg aagagagtaa atcaaaccgc gcaattggcg ttgcgctgaa
       61 tgatactgca tgtcgcgtat tgaaaaaaca aatcgggaat catcaccgtt gggtatttgt
      121 gtataaggaa agctgtacca aaccagacgg aacgaaagcg ccaacagtca ggaagatgcg
      181 gtatgacgca aacacagcct ggaaagcggc gctgagacga gcaggtattg atgatttcag
      241 atttcacgac ttgagacaca cctgggcaag ttggctggtt caagccggag tcccgttatc
      301 agtgttacag gaaatgggag gctgggagtc tatcgaaatg gttcgtcgat atgctcacct
      361 cgcacctaat caccttaccg aacacgcacg gcaaatagac tcgattctga acccatcggt
      421 cccaaatttg tcccagtcaa gaaataagga acgtactaat gatgtgtaac ttattgattt
      481 aaatggataa taggaacaat acacataggc gataagcttt gtttttgttg ccttttttat
      541 tgttcatttt ttatagatac ccccaaaagt acccccatat ctttttccgc ccctgaaatt
      601 tggttgttat tcatacagca tatcaggcgg taacgctctc cctttaaccc acttattgta
      661 tgatagtgct atcccacaat aaggaatcag ccagccatga gaatgaccag tcgcaaaaaa
      721 gagatactta gctactttga accggataac ctcgaatggg tgactggtga gattggtgcg
      781 ccgccgttcg atgtgtcggg tgtggcctac ctgcttcatg gcatggtgtc gttcgataag
      841 cgtcaccagc tcgaatcaac ccggcgcaca ctggaaagca tggttgctgg tggactgctt
      901 gagcgggtga cagtgtatga atcgcgccag atacggcgcg gcggtgatac taacgcaacg
      961 gtcgtgcgct acggtttacc cggtcagtgc gctgttatgc gcgatactgg cggcgctgac
     1021 aacgcaatat caggtgaata catgcgggtc agttaatagc ctgcaatctg tcgttgatgc
     1081 ggctcaggtc gtaaacccgg cgttcaatat ccagcatcag gaaattgtgg atctcggcct
     1141 gctcaggatt atcagactct ctggcctgta atagcaattc attgtaacca tgcaagccgc
     1201 ctgagttttc cgccggggtg gtgcctttcc cacgcaggca gctaatgggc tgattactcg
     1261 tatcgtgtgt ttgcttctcc agcacaatga cgcaatccca ccagtcgcca aagtcatacg
     1321 tgtaaaccag cttgtcaccc tcattctgga gcgccgccca tagtggcgtg attgccgcgt
     1381 tacccatgct ttcatcctca ccccatacgc gatactcgct acctggatca cctttacgcc
     1441 ctttctcgaa catatacagg tgttcgtcat tccagccaaa agctaactgg atcacggcat
     1501 gaagtt
//
LOCUS       DAJTMK010000360         1495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTMK010000360 DAJTMK010000000
VERSION     DAJTMK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.367"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..565
                     /gene="envY"
                     /locus_tag="OO135_004308"
     CDS             <1..565
                     /gene="envY"
                     /locus_tag="OO135_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5399868.1"
                     /translation="LLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAANHSKQQPS
                     KPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQHYWNLRIV
                     ASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQLCGYSSTS
                     YFISVFKAFYGLTPLNYLTKQRQKVMW"
     gene            complement(611..687)
                     /locus_tag="OO135_004309"
     tRNA            complement(611..687)
                     /locus_tag="OO135_004309"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Arg,seq:tct)
     gene            930..1495
                     /gene="fimZ"
                     /locus_tag="OO135_004310"
                     /pseudo
     CDS             930..1495
                     /gene="fimZ"
                     /locus_tag="OO135_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
BASE COUNT          437 a          329 c          320 g          409 t
ORIGIN      
        1 tctgttgttt ttaaataaga acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
       61 ggttatcgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
      121 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gcattacttt ttaccgtatt
      181 gtctaacttt cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag
      241 cgtccgcgac agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
      301 aattgtcgcc agttcgctat gtttaagccc cagcctgctc aaaaagaaat taaaaaacga
      361 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
      421 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
      481 gtactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcaccaa
      541 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
      601 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaaggtcgt
      661 tgctctatcc aactgagcta agggcgcgtt gataccgcaa tgcggtgtaa tcgcgtgaat
      721 tatacggtca acccttgctg agtcaatggc ttttgatctg gttgctgaac aagtgaacga
      781 ccgcgtctga ttttctgatt tatttcgcta tagcggcaaa caaacgcaca ccgctgcgcg
      841 tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc cctaacggaa
      901 aattgtccgc tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc attatggata
      961 ctcatcctat catcagaatg tctattgaag ttctgttgca aaaaacagtg aattgcagat
     1021 tgtcctgaaa acggatgatt accgcataac catcgattat ctccgaaccc gtcctgttga
     1081 tttaatcatt atggatatag acttgcccgg aacagacggt tttaccttcc tgaaaaggat
     1141 caaacaaatc cagagcacag tgaaagtgtt atttttatca tcgaaatcag aatgctttta
     1201 tgctggcaga gcgatacaag ctggtgctaa cggttttgtc agtaaatgca atgatcagaa
     1261 tgatattttt catgccgttc agatgatcct ctccggatac acgttttttc ccagcgaaac
     1321 gcttaactat ataaaaagca ataaatgtag tacgaatagt tcaacggtca ctgtgctatc
     1381 taatcgtgaa gtgaccatat tacgttatct ggttagcgga ttatctaata aagaaattgc
     1441 cgataagtta ttgcttagca ataaaacagt tagtgcgcat aaatctaata tttat
//
LOCUS       DAJTMK010000361         1490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTMK010000361 DAJTMK010000000
VERSION     DAJTMK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.368"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            248..>1490
                     /gene="tnaA"
                     /locus_tag="OO135_004311"
     CDS             248..>1490
                     /gene="tnaA"
                     /locus_tag="OO135_004311"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5399869.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDRE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCP"
BASE COUNT          397 a          345 c          383 g          365 t
ORIGIN      
        1 aataagattg tcgatcacca cccttaattt acccttctgt agccatcacc agagccaaac
       61 cgattagatt caatgtgaac gattagtttg ctatatctta attttgcctt ttgcaaaggc
      121 catctctcgt ttatttactt attttagtaa atgatggtgc ttgtatatat acatggcgaa
      181 ttaatggata ttgcagatgt aatattcaca gggatcactg taattaaaat aaatgaagga
      241 ttatgtaatg gaaaacttta aacatctccc tgaaccgttc cgcattcgtg ttattgagcc
      301 agtaaaacgt actactcgcg cttatcgtga agaagcaatt attaaatccg gtatgaaccc
      361 gttcctgctg gatcgcgaag atgtgtttat cgatttactg accgacagcg gcaccggggc
      421 ggtaacccaa agtatgcagg cagcgatgat gcgcggcgac gaagcctaca gcggcagccg
      481 cagctactat gcgttagccg agtcagtgaa aaatatcttt ggttatcaat atactattcc
      541 gactcaccag ggccgtggcg cagagcaaat ctatattccg gtactgatta aaaaacgcga
      601 gcaggaaaaa ggcctggatc gcagcaaaat ggtggcattc tctaactatt tctttgatac
      661 cacgcagggc catagccaga ttaacggctg taccgtgcgt aacgtctata tcaaagaagc
      721 cttcgatacg ggcgtgcgtt acgactttaa aggcaacttt gacctcgaag gattagaacg
      781 cggtattgaa gaagttggcc cgaataacgt gccgtatatc gttgcaacca tcaccagtaa
      841 ctctgcaggt ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca gcatcgcgaa
      901 gaaatacgat attccggtgg taatggactc cgcacgcttt gctgaaaacg cctatttcat
      961 caagcagcgt gaagcagaat acaaagactg gaccatcgag cagatcaccc gcgaaaccta
     1021 caaatatgcc gatatgctgg cgatgtccgc caagaaagat gcgatggtgc cgatgggcgg
     1081 cttgctgtgc atgaaagacg acagcttctt tgatgtgtac accgagtgca gaaccctttg
     1141 cgtggtgcag gaaggcttcc cgacatatgg cggcctggaa ggcggcgcga tggagcgtct
     1201 ggcggtaggt ctgtatgacg gcatgaatct cgactggctg gcttatcgta tcgcgcaggt
     1261 acagtatctg gtcgatggtc tggaagagat tggcgttgtc tgccagcagg cgggcggtca
     1321 cgcggcattc gttgatgcag gtaaactgct gccgcatatc ccggcagacc agttcccggc
     1381 acaggcgctg gcgtgcgagc tgtataaagt cgccggtatc cgtgcggtag aaattggctc
     1441 tttcctgtta ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg
//
LOCUS       DAJTMK010000362         1455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTMK010000362 DAJTMK010000000
VERSION     DAJTMK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.369"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            90..359
                     /locus_tag="OO135_004312"
     CDS             90..359
                     /locus_tag="OO135_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5399870.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDS
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEGPQPDGEMMM"
     gene            435..650
                     /locus_tag="OO135_004313"
     CDS             435..650
                     /locus_tag="OO135_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5399871.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSLWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            655..999
                     /locus_tag="OO135_004314"
     CDS             655..999
                     /locus_tag="OO135_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5399872.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKSPPKW"
     gene            complement(965..1237)
                     /locus_tag="OO135_004315"
     CDS             complement(965..1237)
                     /locus_tag="OO135_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399873.1"
                     /translation="MDNNTISLQELLDSISRLREDVNTLTVAFSYLAFSIPREQMQST
                     LASIQFESCNPKWSQEQQDSFRRLAVLLDEKYAGKITISADSSENP"
     gene            1343..>1455
                     /locus_tag="OO135_004316"
     CDS             1343..>1455
                     /locus_tag="OO135_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5399874.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGN"
BASE COUNT          382 a          318 c          410 g          345 t
ORIGIN      
        1 cagcgccagt cagtcagccg gagcaaaaac agaagcccca ctcattccgg aacaaccttc
       61 ttaataaccc atttcaacga gaaaatccta tgtcagaaat aaaatcgctg gtcactgctg
      121 aggcagtgaa ggaagtcctg cgctctgaag aagtccggag cgcactgaaa cagcaacttc
      181 ggcagaacct tgaggcgcgt cttgatgcag aagtggattc aattctggat gaactgctgg
      241 gcgcaccgac tgttccggag ccggaaggca ttgcggatga cagtgctgtt tcagatggcg
      301 tcgggtctca gcctgacggt agtgaagggc ctcagcctga cggcgaaatg atgatgtaac
      361 catgcgcagg gggtgtcggt gtgagctgat gccccacttg ttgttgtgag cttccggatt
      421 gcgggagacg gggtatgtac cagatggaaa aaatcacaac aggtgtgtca tacaccacgt
      481 cagcggtggg aacgggctac tggttcctgc agttgctgga cagggtttcc ccgtctctgt
      541 gggcggcaat aggcgtactg gggagtctgc tgtttgggct gctgacatat ctgactaacc
      601 tgtatttcaa aatcagagag gaccgtcgta aggctgcacg gggagagtaa ttcaatgact
      661 caaaactatg aactgattgt gaaagggatc cgcaattttg agaataaagt tacggtaact
      721 ttagcgttac gggacaaaaa acgctttgac ggtgaaattt ttgacctgga catctcgctg
      781 gaccgtgttg aaggtgccgc gctggagttt tatgaggcag cagccagaag gagcatcaga
      841 caggtcttcc tggatgttgc tgccgggtta tgtgaagggg atgagcagtc gccggaaaag
      901 cgccccgtaa ttttagaggc gcaggatgtg ttgataacct acagaggaaa actaccggga
      961 ataattacgg gttctctgaa gagtccgccg aaatggtaat tttaccagca tatttttcat
     1021 ccagtaatac agcaagccgc ctgaaagagt cttgttgttc ctgagaccat ttgggattgc
     1081 atgattcaaa ctggattgat gccagcgttg attgcatctg ttcccttgga attgagaatg
     1141 ccagatatga gaaggcgacg gtaagggtat tcacgtcttc ccgaagcctg gaaatgctgt
     1201 cgagcaactc ctgtagagaa atggtgttat tgtccataaa taatcctcat gattgtattg
     1261 acctgttagc agcctgaggc aacaggctgg aactgataaa catatccagg gctcagaaac
     1321 cgataaatcc tgataaatat ccatgaacgc aaaaatcaga tacggcctgt cggctgccgt
     1381 tctggcgctg attgccgctg gtgcgcctgc gcctgacatt ctcgaccagt ttctggatga
     1441 aaaggaaggt aacca
//
LOCUS       DAJTMK010000363         1425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTMK010000363 DAJTMK010000000
VERSION     DAJTMK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.370"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..138
                     /locus_tag="OO135_004317"
     CDS             <1..138
                     /locus_tag="OO135_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399875.1"
                     /translation="PAMTAIQALAGLITTMVTQGYEYRRDDDMALWSSADLTYSITYE
                     M"
     gene            146..898
                     /locus_tag="OO135_004318"
     CDS             146..898
                     /locus_tag="OO135_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399876.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDADWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVQAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            912..1343
                     /locus_tag="OO135_004319"
     CDS             912..1343
                     /locus_tag="OO135_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5399877.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESCGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            1340..>1425
                     /locus_tag="OO135_004320"
     CDS             1340..>1425
                     /locus_tag="OO135_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399878.1"
                     /translation="MNFALRLAREMGRPDWRAMLAGMTSTEYA"
BASE COUNT          361 a          332 c          437 g          295 t
ORIGIN      
        1 ccggcgatga ctgcgatcca ggcactggca ggactgatta ccacgatggt tacgcagggc
       61 tatgagtatc gtcgtgatga cgatatggcg ttatggagct ctgcggatct gacttattcc
      121 attacatacg agatgtgagg acgatatggc aacaccaaat cccctggagc cagtaaaagg
      181 tgccggtacc actctgtggg tttacaacgg caagggtgat gcttatgcaa acccgttgtc
      241 agacgctgac tggcagcgac tggctaaggt gaaggatctg acgccgggcg agatgacggc
      301 agaatcctac gatgataact acctggatga tgaagacgca gactggaccg cgaccgggca
      361 ggggcagaaa tctgcaggtg ataccagttt tacgctggcc tggaaaccgg gagaggaagg
      421 tcagaaaggg cttataggct ggtttgaaag cggcgatgtc caggcctata aaatccgttt
      481 tccgaatggc acggtggatg tgtttcgtgg ctgggtcagc agtatcggta aggccgtgac
      541 ggcgaaagaa gtgatcaccc gcacggtgaa agtcactaac gtgggtaaac cttctgtagc
      601 ggaagaacgc agcaaaatta cgccggtcag tgcgattaag gtgacgccga catccggtac
      661 ggtggcaaaa gggaaaacaa ccaccctgac ggtttctttt gagccggaaa gtgcaaccga
      721 caagacgttc agagcggttt ccgccgatcc gtcgaaagcc accattagtg tgaaagatat
      781 gacaattacg gtaaacggcg tggcgacagg taaggtgcag atccctgtgg tgagcggaaa
      841 tggtcagttc gccgcagtgg ctgaagtcac cgttactgaa gcgggcgctg cagggtaaac
      901 ggaggtaata catgtttctg aaaacagaac aatttgaata taacggtgtg tctgtcacgc
      961 tttccgaatt gtctgcgctg cagcgtatcg agcatcttgc cctcctgaaa cggcgtgcag
     1021 aacaggcaga atcctgcggc aacctgcagg taagcgtgga agatctcgtc agaaccggcg
     1081 cgtttctggt ggcgatgtcc ctgtggcata accatccaca gaaaacgcag tcaccgtcaa
     1141 tgaatgaggc cgtgatgaag atagagcagg aagtgctcac cacctggcct gccgatgcca
     1201 ttgcccgggc ggaagacgtg gtgttgtgcc tgtccgggat gatcgaagct gttcgtccgg
     1261 atactgatat tactgaagtg gcgaaaaata acacgctgac tgatgatgat ttttctgcgg
     1321 gaaagtcttc gacggcgagc tgaactttgc cctcagactg gcgcgtgaga tggggagacc
     1381 cgactggcgc gccatgcttg ccgggatgac atccaccgaa tatgc
//
LOCUS       DAJTMK010000364         1407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTMK010000364 DAJTMK010000000
VERSION     DAJTMK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.371"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(66..551)
                     /locus_tag="OO135_004321"
     CDS             complement(66..551)
                     /locus_tag="OO135_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HCR5399879.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGATAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDAADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     I"
     gene            complement(580..806)
                     /locus_tag="OO135_004322"
                     /pseudo
     CDS             complement(580..806)
                     /locus_tag="OO135_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(803..1072)
                     /locus_tag="OO135_004323"
     CDS             complement(803..1072)
                     /locus_tag="OO135_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399880.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
BASE COUNT          374 a          358 c          285 g          390 t
ORIGIN      
        1 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggaatc
       61 attacctaaa tattgcgaaa atctattggg aataaacttt cactttcttt atcatacagc
      121 atattacctg tattaagatc gtaatggatt atactcaact cattcaattt acatatcaac
      181 tgtagataaa ggctctctat attatcaggg atatcagctg catcaatgtc actaagggga
      241 gtcccaggca cgcgcagcat tcggaggtac acatctccgc catactgtat aacaacggat
      301 gcttcatcgc cataaaaagc attaaaaagc tcagattctt gccaagccat ttccagaatc
      361 tcattgtact ggttgccaat aagatcatac tttttataca aagcagatga atcattcaca
      421 tcttcaaaga tttctgctgt cgccccctga cctattaact taccgagaat gggctccgtt
      481 actatgccag catgagaatt attattctca aatggaaaag gtgtttgaat aatggtagat
      541 gttattttca tgtcatacct ttgttacgat atttaaaaat cacaagagat tttcacacaa
      601 aataacgtta cacgctacct tgtgtggtcc agatgttcct tccacacgac cattgcggcc
      661 tttatacggc tgccctgcca ggctgctggc tgaataacca atccgttcgt ccggttcaat
      721 ggcggccatc aggttctgct ccgcaatgta ctacgaatgc gcttcctgtc ctggatggcc
      781 agcatggtcc agaacatctc catcatttat cagcaatctt cctgctcatt tcatcccgcc
      841 atgcagcagc agaggattcg accatgtcat ggggaagcac atcaccgcat tcgagttgct
      901 gacggcctgc cgcaacctgg tcacggaacc aagaattgtg ctgccgttca acggtctggc
      961 gcataaaatc ccggattaac tgagagccat tacggtccat gctttttgca gcggccataa
     1021 aagcatcctt cagttctgcg tcgattctta cactcatatt aacctgtgcc atttgccacc
     1081 tcgtcattac cacgtatata cacagtatat aactgttcag atatcgtcac caggatatgc
     1141 cgcgccagcg gcatgtaagg cggcactccg ttgttgcata tggcagagga gtaaaaccgc
     1201 cgaagcccgg cgtaagccgg tacgtattga caggtttcac ctgtcctatc actggcgctg
     1261 acagttttat gcgatgtgat tatgaatggt gcagttgtga gccgttttca gacaatcgca
     1321 gggccagtac ctcgtcagtc agctgacggt aaatctgctg ttcagtctca cgcatcacct
     1381 gtgcaccggc ttccctctcc gcatccg
//
LOCUS       DAJTMK010000365         1401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTMK010000365 DAJTMK010000000
VERSION     DAJTMK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.372"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..307
                     /locus_tag="OO135_004324"
     CDS             <1..307
                     /locus_tag="OO135_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5399881.1"
                     /translation="VNAIERDKALAWVEKNIRVPLSEPQKAGIASFCPYNIGPGKCFP
                     STFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            467..604
                     /locus_tag="OO135_004325"
     CDS             467..604
                     /locus_tag="OO135_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399882.1"
                     /translation="MFDFSKLIREIRVMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            606..1073
                     /locus_tag="OO135_004326"
     CDS             606..1073
                     /locus_tag="OO135_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5399883.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     EQANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGT
                     VREAPTTSGVDNATGPRLADTAERDYFTLRERLMLMQKQLEVAQEYIRTQCLK"
BASE COUNT          345 a          323 c          402 g          331 t
ORIGIN      
        1 ggttaacgcc attgagcgtg ataaggcgct ggcatgggtg gagaaaaaca tcagagtgcc
       61 attgagcgaa ccccagaaag cggggatcgc gtcattctgt ccgtacaaca ttggtcccgg
      121 taagtgtttc ccgtcgacgt tttataaacg aattaatgca ggtgatcgca ggggagcgtg
      181 tgaggcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtattc gttcaaacaa
      241 ctgctacggt caggtatccc gtcgtgacca ggagagcgcg ctggcgtgct ggggtatcga
      301 cagataagca gaatattttg ctgaaaaatg cggtttgctt acacggacgg ataacacgaa
      361 atcctgcgaa ctgacaaaaa ctaagtgaat aaaagtaaaa aacccgtttg ttggctgcaa
      421 gcggggtttt gtgtttcctg actctggaaa agtcaaagga gaaagtgtgt ttgattttag
      481 caaactgatt cgggagattc gagtgatggc tgaaaaatta tccacctgga agttcattct
      541 tatctggctg gtgtttgtga ttatggcctc cggttatttc atcggtcaga tacgctggtg
      601 gtgaaatgaa ccgcgtactg tgcgtggtca tcattgccct gctggtggcc tgtggtgcgc
      661 ttagtctggg gctgaatcat taccgtgata acgccataac ctacaaagag cagcgcgata
      721 aaaaagtcag tgagctggag caggcaaatg caaccattac tgatatgcag cagcgccagc
      781 gtgatgttgc tgcacttgat gccagatact cgagggaatt agccgatgcg agagctgaaa
      841 atgaaactct gcgcgctgac gttgccgctg gtcgtaagcg cctgcggatc aacgccacct
      901 gccccggtac cgtgcgtgaa gcccccacca cctccggcgt ggataatgca accggccccc
      961 gactggcaga caccgctgaa cgggattatt tcactctcag agagcggttg atgctgatgc
     1021 agaagcagct ggaagtggcg caggaatata tccgcactca gtgcctgaaa taagttttgc
     1081 tgatgcgcgg tattgtcgcc gtatccccgc attaacagag accgcagccc gaccgggaga
     1141 ctcctctgcg cgagtgtgcg gggataatca aaaacgatac acaccggggt ttaccgcgtt
     1201 aacggagcgc ggcgttgtcc cctcatagtc gcctgtccgg tgcgatggtg gaagaaaccg
     1261 gactacattg aaaatgataa ccattatcat ttttgcgggt cctttccggc gatccgggcc
     1321 gttacggggc ggcgacctcg cggtttttca ctatttatga aaatttttca gggaaaatcg
     1381 tgtcggtact tctcgaatat a
//
LOCUS       DAJTMK010000366         1398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTMK010000366 DAJTMK010000000
VERSION     DAJTMK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.373"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            8..691
                     /locus_tag="OO135_004327"
     CDS             8..691
                     /locus_tag="OO135_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR5399884.1"
                     /translation="MKTQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            730..>1398
                     /locus_tag="OO135_004328"
     CDS             730..>1398
                     /locus_tag="OO135_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5399885.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPGVGENARRDKEYEYLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTHLHQPSPA
                     QRINI"
BASE COUNT          364 a          333 c          346 g          355 t
ORIGIN      
        1 ccccactatg aaaacacagc atgatcatta tgaccgcctg tccgtcaggc tatcggtcat
       61 tatcagccgc ttactggctg gcgaatctct ggagcttaaa gcgttagctg atgaatttgg
      121 tgtctcagaa cgcaccctac agagggattt tcagcaacgc ctgatccatc tggatctcga
      181 gcattccagc ggaagctacc gacttacctg ccgaacgggc cgggagcact cacccaacac
      241 atttgcgttc atccgtaatt ccgggatttc agggattatt ccggctcaga atcgcaggtt
      301 aattcgactg attaccagta actccggaag ctcaccgtgc cttatagggc atggagggct
      361 gccatctcca gttacacaaa ccgcctgttt tctggaactg gcggaagcta tccgtgacta
      421 ccgcgccgtt acattattag ttaacggtaa aagctacgaa gcagtagctc cccgccgtct
      481 gatatttcag tccaaaaact ggtttcttgt tgctacccgc accagaaatt tacaggtgtt
      541 tcggatggaa gatgttaacg ccgtaaccgt tctggatacc tcttttcgca gaaagcctga
      601 acttgatgct ctgactgcca gtgaagagtt tatttcagcc ctccctcact ttcgtttcat
      661 aagtgacgta attcagacct tcagagagta acagacaacg ttcatttcaa attaaaaagg
      721 agacctcgta tgaatgaccc ggtaaagggc gaaggccgga tgattactga tgccagtggc
      781 gtcaccagaa gggaaggcgg gattttggga atgtggagca atatatttcc agaacgtaat
      841 ggtagcaacg agcaaaaagg tgatagcaag ccgcacatac ccgacatcag ggatattctt
      901 gatattttcc ctcagccact gcgggaaaag ctgttgaaca aaatcagtga agccatcgac
      961 tatgaacccg tgattggtgt catgggaaaa actggcgcgg ggaaaagcag cgtatgtaat
     1021 gcgctgttta aaggggaggt ttgtgcagtt agcgacgttg aggcatgtac tcgcgaagtt
     1081 caggaattgc gtatacgctt cggcaaacat tcgctaaaga tcattgatat acctggtgtc
     1141 ggtgagaatg cccggcggga caaggagtat gaatatttat accgtaattt gcttccctcc
     1201 ctagatctta tcctgtgggt aataaaagga gacgatcggg ccttttctgc ggatgagcat
     1261 ttctacaata atgttctgct ccctgctggt gggggtgagc gcgttttatt cgtcctcaac
     1321 caggttgata aaattgagcc attccgcgag tgggatactc atctgcatca gccatctcct
     1381 gcacagcgca tcaacatc
//
LOCUS       DAJTMK010000367         1394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTMK010000367 DAJTMK010000000
VERSION     DAJTMK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.374"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..550)
                     /locus_tag="OO135_004329"
     CDS             complement(<1..550)
                     /locus_tag="OO135_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399886.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYPGEDAAEQLELMRSVFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLL"
     gene            complement(599..946)
                     /gene="tnpB"
                     /locus_tag="OO135_004330"
     CDS             complement(599..946)
                     /gene="tnpB"
                     /locus_tag="OO135_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5399887.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDAPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(943..1332)
                     /locus_tag="OO135_004331"
     CDS             complement(943..1332)
                     /locus_tag="OO135_004331"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399888.1"
                     /translation="MSDMQKNVNPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT          285 a          384 c          395 g          330 t
ORIGIN      
        1 ccagcagccc cggtcctgcg ataccccgct cgatgggccg tgaaggcgcg ggggcctgca
       61 cgatggcatc gcactgagta caggcatgct tttcacgtac agtccggata acccggaaga
      121 cgctgcgcat caactccagc tgttcggcgg catcctcacc cggatagctc agtgagcctc
      181 cacattccgg gcagcatgac gctgccggca gcagccgttt ttcatcgcgg gggagtgatt
      241 cggggaacgg tttgcgggtg cgggtttgac gcagcgggcg ctgcacggcc gggtcgtcaa
      301 cccgaccggt aagggtatca ctttctttct gaagtgcctt caggtcagct tccatctgtg
      361 cgatacgacg ggagactttt tcggagcggc tgccgaagtt catccggcgc agcttatcca
      421 gctgtgcctg cagatggtct atttcgcgtt cacgctcgtt cagcttttcc agcagggcac
      481 ggttcagcgc ctcctgttcg gcaaggagac gtttcagtgc attgatatcg tcaggaagtg
      541 agctgctcat accgggtata ttaccaggct cattcagcgt cgaccaggat aaagaggctt
      601 acaacatagt cagggacgta agcagtcttt taggctgccg ccagtcgata ccttcaagga
      661 gcatcgccag ctgtgccggt gtgaggaaca ctttgccatc ccgggctgac ggccaggcga
      721 agcggccgcg ctccagccgt ttggtcagca gacacagtcc atcgccggta gaccagagga
      781 gctttacctg actgccatta cgcccacgga agataaaaac gtgacctgac atcggagcgt
      841 ctttcagcgt cgtctgcacc tttgccgcca ggccgttgaa gccatttctc atatcggtga
      901 taccggcaac cagccaaatt ttggtcccgg aaggtaacgg gatcatcgct tcagttcctg
      961 tatcagcaga gtcaggagct tttcgctgac attgccattg aagcggagcg tcccgtgccg
     1021 gaacgttacc tcacagctga tactgagggt ttccggatcc tctgcgagcg attctggctg
     1081 ttcggcagct gcatcgagag tcacaggaag tagctggggg ctctctgaag aaggtaatag
     1141 cagctttccc tcgcgccatt gttgtcgcca tttgaacaac agattggcgt taatgccatt
     1201 ttcaagagca agttttgaga tggatatccc gggttcacag gaggcagcaa cgagctgctg
     1261 tttaaattcg ggaggataat tagggcagcc ttttcgcctg ccgggattca catttttctg
     1321 catatctgac actttggttc ccactactta tttggtggac accactttgt ctaattcgtc
     1381 agattctgac caga
//
LOCUS       DAJTMK010000368         1391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTMK010000368 DAJTMK010000000
VERSION     DAJTMK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.375"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(8..475)
                     /locus_tag="OO135_004332"
     CDS             complement(8..475)
                     /locus_tag="OO135_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399889.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          325 a          306 c          363 g          397 t
ORIGIN      
        1 taagatatta cgttaaattg tgagggaatg gtccccatat actgacttta tcatagggat
       61 agggttggta gtgggtcgat tgggtttcgg cattgtttcc gttaatgcca attgcatttt
      121 tgtatttgat gcaacagaag gcgtgagatt ctcctgggcc tggaaaggct aagtgtttag
      181 aattgatggc aaaatatctt ccgtccgatt tttgaagtag ttgttgtttg gctgtctccc
      241 aaagggtgct gttacaaact tcttgaattt gtagtttttg cagggctctt attactgcgt
      301 tgtcatgttc caattcttta ggggagtgta ttattctctc ttttaataag ctgtttacca
      361 cagggtttag ttgttgtcta aagcacgcag ataatgccat tgctacacaa ttttgttccc
      421 gaggaagcat gtttggcggg gtatgccgtg tttgatgtga tatgtctatt ctcatagaag
      481 gtctctggct tatagcatca acaaatacgg ctataaaaca aaattttcat agagtgattc
      541 aagatcttct tgagatcctt ttttgcggcg cgtaatctgc tgcctgcaaa caaaaaaacc
      601 accgctacca acggtggttt gtttgccgga atctagagct accaactctt tcttccgaag
      661 gtaactggct tggtggagca cagataccaa atactgtcct tctagtgtag ccgcagttag
      721 gccaccactt caagaactct taatatctca atccaccttg tctagttacc aatggctgct
      781 gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      901 tacaccgaac tgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1021 ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg
     1081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagctgcg
     1141 tggccttttc cgggttcttg ttttttgctc acatgtttct tttggtgtta tcccctgatt
     1201 ctgtggataa ccgcattacc gtctttgagt gagctgatac cgctcgccgc agccgaacga
     1261 ccgagcggag cgagtcagtg agcgaggaag cggaaaagcg cctggacgag cattttctcc
     1321 ttacgcatct gtgcggcatt tcacacccgg catggtgcac ttttcataca atcctcactg
     1381 atgccaaatg a
//
LOCUS       DAJTMK010000369         1387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTMK010000369 DAJTMK010000000
VERSION     DAJTMK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.376"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(493..759)
                     /locus_tag="OO135_004333"
     CDS             complement(493..759)
                     /locus_tag="OO135_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399890.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQWHSNI"
     gene            complement(941..1339)
                     /locus_tag="OO135_004334"
                     /pseudo
     CDS             complement(941..1339)
                     /locus_tag="OO135_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          360 a          297 c          291 g          439 t
ORIGIN      
        1 cccgcaaaat gagtcaggga aaacgacaca atcaggcgct tatcgccctg gcgagacgac
       61 gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg caggggtcat
      121 aacatgcttg acaacttaat agggcccccc cccaaagccg tctatgggaa ggagtctgct
      181 gttattccag agaatggtaa ttcagtcata tatttctcca tctatgactt gtcaatacca
      241 tcctgatatt tgtgggtcag caatatattc tactaaaagg tatttaaggt cattgcgtcc
      301 ctgcactcga ataatggtga agaaagcatg atgaatatat cacgataacg ttcagtatgt
      361 atcgatgata gatggagtta ctcctctctg aaatagaatt actgctctac tttttctgtt
      421 tccagaaatt attagtaaca aaccctgata tattcttttg aaaatggagc ttaaacgaat
      481 ctgaaattaa cttcagatat tagaatgcca ttggctttgt ttttcaacaa ttcctacttc
      541 atttatcttt gtcaaccatt gcatttctgt agcagtgaat ttcaacgttt tcgtccggtt
      601 ggcagcaggt atttgtaatt cgtatatggc tgctttttta ttgaaaacag tatatccatc
      661 tgtagtaaca gtagaagata ataatacagg atttgacact tcaaggatga tattttcttg
      721 tttttgtgga aggcaaggga atatagtttg tgtaagcata ttgcttcctt atgttatatt
      781 ttagtaaaga atttattttt tacattatag tcggctaaat gaagtttact gtaaaaaaac
      841 gatacttttt tcgtaagcga tgagatgtgg agccagaaaa atggctgcgt tacgtcattg
      901 aactgtcctt gacccacgat attcagacag tcgttgtatc ttaagttgac attacccgct
      961 gacgtcgata ttcccttggt gagagataac ccaacgcact atgcggatgg ttttcattgt
     1021 aatgctcgat cgccactgca aggttatgca atgccgttct tggtttcggc atgaacgcga
     1081 tgcagtcttc cttcatcgtt ttcacgaacc gttctgctat tccattactc tgagggctac
     1141 tgactgccgt cgtgcagggc tccagattca actctctggc gaactgccgt gtttcatgag
     1201 ccgtataagc cgaaccgtta tcggccactg gattggccgt tccggtcctt tatcgccata
     1261 tcgctgacat gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt
     1321 ctatggcttc acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg
     1381 gcattcg
//
LOCUS       DAJTMK010000370         1387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTMK010000370 DAJTMK010000000
VERSION     DAJTMK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.377"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..888)
                     /locus_tag="OO135_004335"
     CDS             complement(<1..888)
                     /locus_tag="OO135_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5399891.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGG"
     gene            complement(1069..1387)
                     /locus_tag="OO135_004336"
                     /pseudo
     CDS             complement(1069..1387)
                     /locus_tag="OO135_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT          380 a          318 c          325 g          364 t
ORIGIN      
        1 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
       61 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
      121 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
      181 cacaatccct tttagctgcc ctgcaacata caccggcgtc agcaaactca tcacgttctg
      241 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
      301 aatatctttg cgatcgatac caatacttcc tgactgcagg aaggaccagt tattcatcgc
      361 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
      421 atagtgaaat ttctcttcgg ccatcccaag acctgaaccg taatcatcct ggtggcggtt
      481 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
      541 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatgag cagtcaggtt
      601 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
      661 cgacacttcc gactgctcag cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt
      721 ctgatactta tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc
      781 actcattgct ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat
      841 gacacttgcg gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac
      901 tccttcggtt agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc
      961 gctaattata ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa
     1021 ttgtatgcaa aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatatgatg
     1081 aataatcact ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt
     1141 catgacaagc gagatattac gcgtgtcacc cgttgaaaaa accgtgccaa tactgatggt
     1201 catttgttca ggaacatcat atctgttact aaagccggtc aggagttcga catttttatg
     1261 tatacgatcc gcaatgattt ttgcctaggc actattgagt tcggtaaata gcaagccaaa
     1321 cacctcaccc tccagtcgcg ctaaaatatc atcagggcgg atgctttgct ggataatatc
     1381 gacaatt
//
LOCUS       DAJTMK010000371         1382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTMK010000371 DAJTMK010000000
VERSION     DAJTMK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.378"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>1382
                     /locus_tag="OO135_004337"
     CDS             <1..>1382
                     /locus_tag="OO135_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5399892.1"
                     /translation="VDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNA
                     DLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTEQDNSSVDNVVAATGNYK
                     VRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKADLGAYTYQAEQRGNTVVLQQ
                     MELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDN
                     GTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYS
                     SAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAG
                     YVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYF
                     KLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVD
                     QDWSANVGNAA"
BASE COUNT          354 a          342 c          349 g          337 t
ORIGIN      
        1 gttgatcagt tcgatactga agctttccgt actgataaac tggaactgac cagcggcaac
       61 attgctgacc ataatggcaa cgtaatttct ggtgtgttcg acatcaacag cagcgattac
      121 gttctgaacg ctgatctggt gaacgaccgt acgtgggata cttccaagtc taactacggt
      181 tacggtattg ttgctatgaa ctctgatggt cacctgacta tcaatggtaa cggcgacgta
      241 gacaacggta ctgaacagga taacagctct gttgataacg ttgttgctgc aaccggtaac
      301 tacaaagttc gtatcgacaa cgcaactggc gctggcacta tcgctgatta caaagataaa
      361 gaacttattc gggtaaacga cgctaacacc aacgcgacct tctctgctgc taacaaagct
      421 gacctgggtg catacaccta tcaggctgaa cagcgcggta acaccgttgt tctgcaacag
      481 atggagctga ccgactacgc taacatggcg ctgagcatcc cgtctgcgaa caccaatatc
      541 tggaacctgg aacaagacac cgttggtact cgtctgacca actctcgtca tggcctggct
      601 gataacggcg gcgcatgggt aagctacttc ggtggtaact tcaacggcga caacggcacc
      661 atcaactatg atcaggatgt taacggcatc atggtcggtg ttgataccaa aattgacggt
      721 aacaacgcta agtggatcgt cggtgcggct gcaggcttcg ctaaaggtga catgaatgac
      781 cgttctggtc aggtggatca agacagccag actgcctaca tctactcttc tgctcacttc
      841 gcgaacaacg tctttgttga tggtagcttg agctactctc acttcaacaa cgacctgtct
      901 gcaaccatga gcaacggtac ttacgttgac ggtagcacca actctgacgc gtggggcttc
      961 ggtttgaaag ccggttacga cttcaaactg ggtgatgctg gttacgtgac tccttacggc
     1021 agcgtttctg gtctgttcca gtctggtgat gactaccagc tgagcaacga catgaaagtt
     1081 gacggtcagt cttacgacag catgcgttat gaactgggtg tagatgcagg ttataccttc
     1141 acctacagcg aagatcaggc tctgactccg tacttcaaac tggcttacgt ctacgacgac
     1201 tcaaacaacg ataacgatgt gaacggtgat tccatcgata acggtactga agggtctgcg
     1261 gtacgtgttg gtctgggtac tcagttcagc ttcaccaaga acttcagcgc ctataccgat
     1321 gctaactacc tcggtggtgg tgacgtagat caagactggt ccgcgaacgt gggtaatgct
     1381 gc
//
LOCUS       DAJTMK010000372         1305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTMK010000372 DAJTMK010000000
VERSION     DAJTMK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.379"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..165)
                     /locus_tag="OO135_004338"
     CDS             complement(<1..165)
                     /locus_tag="OO135_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399893.1"
                     /translation="MSGNNNTITAADAIITLTVNNLYPSGVRLQGFATDNVYGTDPLV
                     LAETARGIDGK"
     gene            complement(166..>1305)
                     /locus_tag="OO135_004339"
     CDS             complement(166..>1305)
                     /locus_tag="OO135_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HCR5399894.1"
                     /translation="MLSINGKSVSIEVNFNGVASFAAAATALQTALTAAVATVVFDTT
                     QNAFVITVAGAKPGSTTITFGSGSAAELLKMTSNTGAVISRGAPVSDVPDTMAAIKAA
                     SQQWAGFSTVSEVTDEQHLAFSAWANGQGKRYFYVAWTTSGNARVKGSTEHIAYQIIA
                     VNNYSSVVPVFATDGNRAAAVLGYAACLDFIRPEGRVSFKFREYEGLAADVTSGSDYD
                     ALIAAGYNFYGKYAENSVVEDYWADGTITGDFKWLDSFCGQIWLNASLQGAVIALFKS
                     NQTVPYNNEGRALVAASMSDVIQQYKRWGGIREGVTLTEAQKKQINNVVEADVSSTLF
                     ATGYYLYIGDMLPSLRATRSSPSCTLWYCDGGSIQKLVIASTEVQ"
BASE COUNT          303 a          375 c          311 g          316 t
ORIGIN      
        1 tttaccgtca ataccgcggg cagtttccgc cagtaccagt ggatcggtgc cataaacgtt
       61 atctgttgca aatccctgaa gtcgtacgcc ggagggatac agattattca ctgtcagcgt
      121 gataatggca tccgccgcag tgatggtgtt gttgttaccc gacatttact ggacctccgt
      181 ggatgcaata acaagtttct ggatactacc gccgtcacag taccagagcg tacaggacgg
      241 actgctacgg gttgcccgca aagagggaag catatcgccg atatacaggt aatagccggt
      301 ggcaaacaac gttgaagaaa catctgcctc cacaacattg ttaatctgtt tcttctgcgc
      361 ctccgtcagt gtcacccctt cacggatccc tccccagcgt ttgtactgct ggataacgtc
      421 actcattgac gctgcaacca gcgcccgccc ttcattgttg taggggaccg tctggtttga
      481 cttgaataat gcgatcaccg ccccctgcaa actggcattc agccagattt gcccgcagaa
      541 actgtccagc catttaaaat caccggtaat agtgccatcc gcccagtaat cttccaccac
      601 actgttttcc gcatattttc cgtagaaatt gtaacctgcg gcgatcagcg catcataatc
      661 gctgccactg gtaacatcag cggccagccc ctcatactcg cggaacttga atgatacacg
      721 cccctctggt cggataaaat caaggcacgc tgcatacccc agtaccgctg cagcccggtt
      781 accatcagtc gcgaaaacag gtacaacaga gctgtagtta ttgacagcaa ttatctggta
      841 agcgatgtgt tcagtactgc cttttactct ggcgttgccg ctggttgtcc atgccacata
      901 aaagtaacgc ttgccctgcc cgtttgccca tgcagaaaac gccaggtgtt gctcgtcagt
      961 gacctcagat accgtggaaa aacccgccca ttgctgggaa gcggccttaa tggctgccat
     1021 tgtgtcaggg acatcagata caggcgcgcc ccgggatatc accgcaccgg tgttactggt
     1081 catcttcagg agttctgctg ccgatccact gccgaacgtt atcgtggtac tcccgggttt
     1141 cgccccggcg acagtaatga caaaagcatt ctgtgtggta tcgaatacca ctgtagccac
     1201 tgctgcggtc agcgctgtct gtagtgccgt tgcagcagcg gcgaagctgg cgacaccgtt
     1261 aaaattcact tcaatgctga cgcttttccc gttaatactc agcat
//
LOCUS       DAJTMK010000373         1290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTMK010000373 DAJTMK010000000
VERSION     DAJTMK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.380"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1256
                     /locus_tag="OO135_004340"
     CDS             <1..1256
                     /locus_tag="OO135_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5399895.1"
                     /translation="VAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAAFV
                     TTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTPRAGGIVLPIINSVAV
                     ALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWGG
                     WALATGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVF
                     VLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGL
                     SSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPV
                     FAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVL
                     TILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          275 a          302 c          342 g          371 t
ORIGIN      
        1 ccgttgctgc ctcaatggtg gtggtcggta acttatccga cggtgcgttt aagaccaccg
       61 ccgtattaag cggttactct tcaggtacta cctggctggt gttctcggcg tttaccttaa
      121 gcgccgcatt tgtgaccacc ggtttaggta aacgtattgc ctatctgctg attggtaaaa
      181 tcggtaacac cacgctgggt ctgggttacg ttacggtatt cctcgatctg gtactggctc
      241 cggcaacacc gtctaacacc ccgcgtgcgg gcggcattgt gttaccgatc atcaacagcg
      301 tggcggtggc tttggggtcc gaaccggaaa aaagtccgcg tcgtgtcgga cattacctga
      361 tgatgtccat ttacatggtc accaaaacta ccagctatat gttctttacc gcaatggcgg
      421 ggaacattct ggcgctgaaa atgatcaacg acattctgca cctgcaaatt agctggggtg
      481 gatgggcgct ggccaccgga ttgccgggca tcattatgct gctggtcacc ccgctggtaa
      541 tttacaccat gtatccacca gaaattaaaa aggtggataa caaaaccatc gctaaagcgg
      601 gccttgccga actgggaccg atgaaaatcc gcgaaaaaat gctgctcggt gtctttgtgc
      661 tggcgctact gggctggatt ttcagtaagt ctctgggggt tgatgaatcc actgtggcaa
      721 tcgttgttat ggcgaccatg ctgctgctgg gtatcgttac ctgggaagac gtggttaaaa
      781 ataaaggcgg ctggaatacc ttaatctggt acggcggtat tatcggatta agctccttat
      841 tatcgaaagt taaattcttc gaatggttag ctgaagtctt taaaaataac ctggcatttg
      901 atggtcacgg taacgttgct ttcttcgtta ttattttcct cagcattatc gtgcgttatt
      961 tcttcgcttc cggtagtgcc tatatcgttg ctatgttacc ggtatttgcc atgctggcga
     1021 acgtctccgg cgcaccgtta atgttaaccg cgctggcact gttgttctcc aactcctatg
     1081 gcggcatggt tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttata
     1141 acgatattaa atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcctggtgc
     1201 atatcaccct cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat
     1261 tctcgtcata cttcaagttg catgtgctgc
//
LOCUS       DAJTMK010000374         1272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTMK010000374 DAJTMK010000000
VERSION     DAJTMK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.381"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(150..980)
                     /locus_tag="OO135_004341"
     CDS             complement(150..980)
                     /locus_tag="OO135_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5399896.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNKLTREYEYARMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
BASE COUNT          377 a          217 c          238 g          440 t
ORIGIN      
        1 gacgcctgac tgattatgat aagtgcaagg gtaaacgttc cttgcgcttt cttaaattaa
       61 gaagtcgcaa tgagtattac tttgtaaatt gcagggtatt gtttaaatat ctatataacc
      121 tgaatgttag tactcattct tctcagtggt tatttgccaa tataattcca ttcaccattt
      181 tttaattcaa acagttctgg aagagatgac ggttgcagag tcatgcgttt gagtggcgca
      241 tcttcatcac cggggataac gttatatccc atttttgcat ggatctccgc ggtaccagga
      301 taggcttcta taccaattat tgggtcattt cccattccaa tattagcatt aagtaatgct
      361 gcaagggcga atccggaaga atttgccacc cctgagacag ttattatctg atcaatatag
      421 atatcaattc ttggctgctc attggtccat ctttttgcct gagaagctgg aagggttgaa
      481 atatatccta tcgtggttcc ggcataacca ggaacattgc aaccagggcc taatgttatt
      541 tcatgaactg tatagtcctc aacaggatcg aaagggctct tcataaataa atttagccgt
      601 aaaccttctg ctaactctgt agcagcatgc gcctttttac atataccacc atatatttcg
      661 tctaaagctg aggtgatttt caagtttcga tagagcatac gtcctgttgt tgtaatacca
      721 ttgaattctc tgcatgtatc tgtcgcataa ttaaatggcc aggactgttt gccaatatta
      781 agttttctaa tttcgacaac gctttttcca tcgtaattat ggttatttcc acgagtaaga
      841 ggcagaagat agatattttc cgtatccatc cgtgcatatt catattcacg tgttaactta
      901 ttgtcgactt ttggatgatg aaggaagtga ttatctgtaa taatttgact ctgaagggca
      961 ttgcttaatg gcatagacat aatagttacc ttttatgagt atttcaccga tgtttagaaa
     1021 aatagataaa tttttctgta gtaaaaaaga agtaaaaaaa tgacatgcat gtttctgttg
     1081 tagtgatatc aactctacac ggtgatatta aagggtagga aacactctaa agtatcaaaa
     1141 aacgctcatt tgaaattatt ttcatgcaat taaaaagcat atcttattac taattggaat
     1201 ttgatgttgc tatattgatg tctatattaa taatgcctat gaatggtatt tttgatgtat
     1261 ttgatatgtt at
//
LOCUS       DAJTMK010000375         1270 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTMK010000375 DAJTMK010000000
VERSION     DAJTMK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.382"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..185)
                     /locus_tag="OO135_004342"
                     /pseudo
     CDS             complement(1..185)
                     /locus_tag="OO135_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(321..569)
                     /locus_tag="OO135_004343"
     CDS             complement(321..569)
                     /locus_tag="OO135_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5399897.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(924..>1270)
                     /locus_tag="OO135_004344"
     CDS             complement(924..>1270)
                     /locus_tag="OO135_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5399898.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          335 a          285 c          298 g          352 t
ORIGIN      
        1 ggcaagagcg ccacatcggg gatttttctg attggtgccg cgcatacagc gatgaaaagg
       61 agatatttgc tcagtgagac tgaccgaaac ctttccgcca gcatcgtcag cttctgccca
      121 gtaaaaagag acgcggaaaa tgcacaacag gcaccacacg tcatgcatgg attcagattg
      181 ctcataattc accagtacag ctataaatcg taaagagaac agcggtacat cgtatgtaag
      241 aacgtaccgc ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccatccg
      301 ttacagattt tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct
      361 ttcagagttt cctgaacaaa agttttagct gaatctttat cggcggtgcg cgtaacagaa
      421 aggccatcgt tgctggtagc ttttacgatc acctctacat cgtcataacg tttactgatg
      481 cgtcgggtta attcttcctt taacgcatcc acagcaccgg ttggcatttt agtcattttt
      541 tctttggcta tgcagatttc aatacgcata aaagtccctc tacactgtgt ttgtatacag
      601 tatcaatttt aactgtatgg ataaacagta tcaagggtga aggaaagtga ttttttgggg
      661 catgcatggg gcaaaaatat ttgttgtggg gcattttggg gcatgattgg gacactttgt
      721 tacatatgaa ctttgccgat ttttatatga catggaaata caacgctttg attcttctaa
      781 ataaaaaaca tgctcttggg cgttctttag tgatttttaa aaattccgta tcacgcagtt
      841 aaagtggcgg gcatactctt caaggctggt gatccccaac cgcacccatt taggatgcga
      901 ccattgcggt agaccgatgt aaatcataac gcgtgaagga tctcttccac gctacgcaca
      961 cgggcgatgc gcgggtagat atgattaatg ctgttattgt gttgctcggc gctggcggca
     1021 ctacaggcgt cttcggcaat caccagatta aagccaagtt cccaggcatt gcgggcggtg
     1081 gattcaacac cgatattggt cgagatccca cataacacta ttgtatcgat acctcggcgg
     1141 cgcagttgca actccagatc cgtaccgtaa aacgcacccc actgacgttt gatgatttcg
     1201 atatcactgt cagttgcacc taatgcagct ggatgttgcc accagttttc gggcaacact
     1261 ttagcggggg
//
LOCUS       DAJTMK010000376         1262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTMK010000376 DAJTMK010000000
VERSION     DAJTMK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.383"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..437
                     /locus_tag="OO135_004345"
                     /pseudo
     CDS             1..437
                     /locus_tag="OO135_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT          379 a          270 c          252 g          361 t
ORIGIN      
        1 ttcctaaagg tagtagtact tatctgctga tgcgagaaac acattacaat gcgggtatat
       61 tcgtttgggt gttaatgttt tcacgcctga ttataaaaca ccgttatagt gatccttcta
      121 ttgtgccacc gccacctgcc tggcaaatga aagcggcttc gctaatgcac atcatgcttt
      181 atataacctt ccttgcactt cctctgctgg ggattgcttt gatggcttac agtggaaaat
      241 cgtggagttt ccttggtttc aatgtgtctc cctttgttac cccaaacagc gaaattaaag
      301 cactgatata aaatattcac gaaacctggg caaatatagg ttacttttta atcgcagctc
      361 acgctggcgc agctctcttt catcactaca ttcagaaaga taatactctg ttacgaatga
      421 tgcctcgccg caaataagtc gatcccctga caaggaaatg tgaaccatgg acatcaacgc
      481 gtcataaatg tttacgcaag tgcacaatgc accctttcca ctatcaacaa ggaggagcag
      541 aaactgtgta aatcgtacaa tggtaagact tatcaaaaat acaggttaat tttggtgttt
      601 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
      661 actcttggaa attatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
      721 gactcaatta ttgacccctt ctctacgcaa cttcagttcc caccaccaac tttgcggcag
      781 tttcatagga tgaatgtcta aaagaataat ggtgagcgat aagaaaacga ctgaataact
      841 gcacattttc gctcgaaagc ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac
      901 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
      961 ttaggaaaag taaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     1021 ggatttgccc ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     1081 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     1141 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     1201 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     1261 gc
//
LOCUS       DAJTMK010000377         1260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTMK010000377 DAJTMK010000000
VERSION     DAJTMK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.384"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..1260)
                     /locus_tag="OO135_004346"
                     /pseudo
     CDS             complement(1..1260)
                     /locus_tag="OO135_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          372 a          354 c          279 g          255 t
ORIGIN      
        1 ccaaccagta aaacgcttcc gttttcaacg caccccacag catcggccag aaaatgagtc
       61 ctgcaattgc ccctgtgcgc ataatagcgc gggcaaatcc gaccccagta gggcgaatag
      121 cgggaagata agagagtgtg gcaatggtca tgcccagccc accaggaccc gctgaatgga
      181 agaaaataat tgatcccaat aaccctaaag acagccaggg attagtcgtt gccagtgcgc
      241 ctaacgacag caatgctaag gtcacaacag caaatccata catcgactgt cggcgagtac
      301 cagtcgttgg agaataaacg agccgatcat cccgcctgtc accccacaaa gggcattcac
      361 gacggcagaa ccggtcaacg cgccagtaag cccgccactt attattcccg ccaacgtaag
      421 aggaagataa acacctacgg cgttatattg ccacgcctgc atggttgcca cgacacagcc
      481 aagaatggtg cgtttgcggt aacgatcatt aaataagatg ccatatccac cactgtattt
      541 attctctgct ttttccgaga atttcgcttc tgttgtaccc gatgccgcaa cgcgagcctg
      601 aacaccataa cgttttccca gaatgtcgca cgcttcctgg taacgcccga cgcgtgcggc
      661 ccacattgcg gattcaccaa taaagaagta acgcaaaatc atgaagatga ctgcaaaaat
      721 agctccgatg aatattccgt aacgccacaa ttgttcttca gcgatacccg acagaagcag
      781 cggcaaaagt accaaataaa caacggttgt cgaaacatac caggccattt gccagaggtt
      841 gaccgacgtt ccggttttct tcgacgttga accacgaagc tctgccacag cattggttgc
      901 caaagggaag tcgatgccta aaccaaagcc catgatgacg cggcacacca gcacccacca
      961 gatactggga gcaatagcta ccgctgcagc accgatggtg cacagcccca tgccaataat
     1021 aaatgcctgc ttttgcccaa agcggtttat tactcggttc gctaataaac cgccaattaa
     1081 cgcggcgatt aatactgatg cattgacggt tgcagcaaga cccggcgaaa tgccgaattg
     1141 agcggcaatg tatttattac caaaccccac catcgccgcc tagtaggcat caatcaatat
     1201 gccgcccaaa gcaataaaaa taacgccgat actggtgttg attgcagagt tcttattaac
//
LOCUS       DAJTMK010000378         1250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTMK010000378 DAJTMK010000000
VERSION     DAJTMK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.385"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>1250
                     /locus_tag="OO135_004347"
     CDS             <1..>1250
                     /locus_tag="OO135_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5399899.1"
                     /translation="AFAFLSLLFWAIIIKYDVSLPTKKVTGILLLIVISGSLFSACQF
                     AYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRD
                     TGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVPL
                     SLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVY
                     VKGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACY
                     DNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDLTKKNVYFHGGREASQQAYHVP
                     MFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDSR
                     VVDANHDVFDYVMFRKEFTEDKQG"
BASE COUNT          357 a          261 c          271 g          361 t
ORIGIN      
        1 tgcctttgct tttttatccc ttcttttttg ggcaataata ataaaatatg atgtttcctt
       61 gccgacaaaa aaagtgacag gaatattatt gctgattgtc atttcgggca gtttattttc
      121 cgcttgtcaa tttgcttata aagatgcaaa aaataaaaaa acgttcagtc catatatact
      181 agcgtcgcga tttgctacct atacgccgtt tttcaatctc aactattttg ctttagcagc
      241 gaaagagcat caaagattac tctcaattgc aaacacggtg ccgtattttc aattatcagt
      301 cagggataca ggtattgata cctacgtgtt gattgtgggg gagtctgtac gtgtcgacaa
      361 tatgtctttg tatggatata cacgctctac gacaccgcaa gttgaagcac aaagaaaaca
      421 gatcaaactg tttaatcaag caataagcgg cgcaccttac actgcgctgt cggttcccct
      481 ttctttaact gctgattctg ttttgagtca tgacattcat aattaccccg acaacattat
      541 taatatggct aatcaagcag gatttcagac tttctggcta agctcgcaat ccgcttttcg
      601 gcagaatggt acagcagtta ccagtatcgc catgcgcgcc atggaaacag tttatgtcaa
      661 aggatttgat gaattgttgt tgccgcattt atcgcaagca ttacagcaaa atacgcagca
      721 aaagaaactg attgttcttc atttaaatgg aagccatgaa ccggcttgta gcgcctatcc
      781 gcaatccagc gccgtgtttc aaccgcagga cgatcaggat gcctgctatg acaactccat
      841 tcattacaca gatagtttgc taggtcaggt ttttgaatta ttaaaagatc gccgcgcctc
      901 ggtcatgtat tttgccgacc acggcctgga acgtgacctt acgaagaaga acgtctattt
      961 tcatggaggc agggaggcta gccagcaggc atatcatgtc ccgatgttta tctggtatag
     1021 ccccgttctt ggggatggcg tggatcgcac aacggaaaac aacatctttt cgacagctta
     1081 caataattac cttattaatg cgtggatggg ggtaacaaag ccggaacagc cgcaaacgct
     1141 tgaggaagtg attgcacact ataaaggaga ctcacgggtt gtggatgcaa accatgatgt
     1201 tttcgattat gtgatgttca gaaaggagtt tacagaggat aagcaaggta
//
LOCUS       DAJTMK010000379         1234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTMK010000379 DAJTMK010000000
VERSION     DAJTMK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.386"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..90
                     /locus_tag="OO135_004348"
     CDS             <1..90
                     /locus_tag="OO135_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5399900.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            93..167
                     /gene="yoaJ"
                     /locus_tag="OO135_004349"
     CDS             93..167
                     /gene="yoaJ"
                     /locus_tag="OO135_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5399901.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(226..474)
                     /locus_tag="OO135_004350"
     CDS             complement(226..474)
                     /locus_tag="OO135_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5399902.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(622..804)
                     /locus_tag="OO135_004351"
     CDS             complement(622..804)
                     /locus_tag="OO135_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR5399903.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(808..1168)
                     /gene="yeaR"
                     /locus_tag="OO135_004352"
                     /pseudo
     CDS             complement(808..1168)
                     /gene="yeaR"
                     /locus_tag="OO135_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
BASE COUNT          325 a          299 c          275 g          335 t
ORIGIN      
        1 ggtattattt ttccggttgt aatctttatt acagcggtcg tatttttagc atggtttttt
       61 attggcggct atgctgcccc gggggcataa agatgaaaaa aacaacgatt attatgatgg
      121 gtgtggcgat tattgtcgta ctcggcactg agctgggatg gtggtaacgt cacctctaaa
      181 aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt aagcgttaac ttttaatctt
      241 cctgtagata aatagcacga caatcgcacc aataacggca accacgaagc tgccaaaatt
      301 gaagccatcg actttaccaa agccaaacag cgtgctgatc catccgccga ctacggcacc
      361 gactatcccc agcaggatag tcataaagaa tccaccacca tctttacctg gcatgatcca
      421 cttcgccaga ataccggcaa taagcccaaa aataatccat gacagaatgc ccattgtttc
      481 ctcacttatc tgttttgcat tagcgggtta gtcgctgata aaaagcatag cacaacatcg
      541 ggagggcaag atttgtgacg agcatcacgg aggatttttt tgcgatggcg cagaaattgc
      601 gccatcaacg atcagtgata attaccaacc acaaacatca tgttcgtttt ccgtgtcata
      661 agaacgcacg gtattcacca gatcttttat cacttcagcc gccacttctg gcaccagcaa
      721 agtcatcggc gtctctgttt cataatcgac agaaacgcca ttactgttat tggtgacggt
      781 cacggtatac gttgctttgc ccatgattca tttcccgtta tgaatgactt tccgttgttg
      841 cgcaccttcc atcaggactt caggagccac gaagaagtca atgttgaaat aagtatcgtc
      901 agtcatggct tcaatgttgt gccacttttc tggagggaac accgcaaact gccccgcttc
      961 gataaggatc acctgatcag gctctgcact gtgttcatca gcgtagccga gatatttgac
     1021 cgccccatgc ataacggaaa ggcgtgggta acccccctgg cgcgttcctt tatcaagatg
     1081 acgttcgaat tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat
     1141 gaatataatt ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa
     1201 cgtgaggtgt actggcaata gcggacacta ccat
//
LOCUS       DAJTMK010000380         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTMK010000380 DAJTMK010000000
VERSION     DAJTMK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.387"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(83..1057)
                     /gene="cysB"
                     /locus_tag="OO135_004353"
     CDS             complement(83..1057)
                     /gene="cysB"
                     /locus_tag="OO135_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5399904.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFFRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          345 a          281 c          285 g          321 t
ORIGIN      
        1 gcacggtata gaaataagta acaaaagaga atatattccg gcaccttcgc tacataaaag
       61 gtgccgaaaa taacgcaaga aattattttt ccggcagttt tatatcttta aacatgacct
      121 caatttcttc attagagcgc aatgcgacag ccgcatcaac gacatcacgc gttaaatgcg
      181 gtgcaaaacg ctgaatgaaa tcatacatat aactgcgaaa aaaagtacta cggcgaaaac
      241 caattttggt tgtactgtgg ctgaagatat cgtgagcatc aacgcgcaca aggtccggat
      301 cggcgaccgg atccaccgcc atgctggcaa tgacccctac ccccagccct aaccggacgt
      361 aagttttaat gacgtcagca tccgttgccg tgaaaacgat acgcggcgtt aaccctgcgc
      421 ggttaaaggc agtatccagt tctgaacgtc cggtaaagcc gaaggtatat gtcaccaacg
      481 gatattgcgc cagttcttca atggtaatgg cttttttgcc tgccagcggg tgatccggag
      541 tgactacaat agcccgattc cagtggtagc acggtaacat cactaaatct tcatacagat
      601 gcagcgcttc tgtggcgata gcgaaatcgg cattgccttt agagacggca tcagcaattt
      661 gtgtcggcga gccctggtgc atatgcaaag aaacgcgagg ataacgctca ataaagcctt
      721 tgatgacgtt tggtaatgcg tagcgtgcct gggtatgcgt ggtggcgata tacagtgagc
      781 ctttatccgg ccaggtgtgc tctccggcaa ccgattttat ggcatcgact ttcgacagga
      841 cttcgcgagc gatacgaatt atttcttgcc ctgctggcgt tacctgcgtc aggtgcttgc
      901 cgcttcggga aaaaatttga atgcctagct cgtcttccag cattctgact tgtttactga
      961 tcccgggttg tgatgtgtaa agtccttccg ctgttgatga gacattcagg ttatgattga
     1021 ccacctcaac aatatagcga agttgttgta atttcatgtt aaaccatcca cttagacgcc
     1081 atcagaacat cgttttttcg tcacacctgg cgggtgacta atacgattta ataataaaaa
     1141 aggtgctaac tataaccact atatcattta tagccttagt gtataggtgt gaataaaaaa
     1201 taatatgcaa taaaaaaggg ctgctttcgc ga
//
LOCUS       DAJTMK010000381         1217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTMK010000381 DAJTMK010000000
VERSION     DAJTMK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.388"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(1..185)
                     /locus_tag="OO135_004354"
                     /pseudo
     CDS             complement(1..185)
                     /locus_tag="OO135_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(253..735)
                     /locus_tag="OO135_004355"
     CDS             complement(253..735)
                     /locus_tag="OO135_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5399905.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(726..1028)
                     /locus_tag="OO135_004356"
     CDS             complement(726..1028)
                     /locus_tag="OO135_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5399906.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          347 a          296 c          256 g          318 t
ORIGIN      
        1 tcagccactg tgcgggtgcc tggtgtattg agcccttttc ttgcggcggt aacccattgt
       61 cctaaagtgc cttaaggaac agataatcgg gaagcgcctt cactgatcga aagctgattt
      121 tcaggaaccg ttttgacggc ttcggcttta aactccgtag agtaacgttg ggtttttctg
      181 ctcattattg gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag
      241 gctacctcag actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa
      301 accaaaacgc tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt
      361 aatgccaacc tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt
      421 accgaaacca tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg
      481 gacaggattg ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc
      541 agtagctaat gagtagtatc ccacaacacg taaagtatcc tccaagcaaa taacgaacgt
      601 gcgagaagca ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac
      661 ctcatttcca cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt
      721 gacatttatt tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc
      781 acaggagctt ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa
      841 cgttgatcca ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta
      901 cggttcttat tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc
      961 cggatgttaa tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa
     1021 acctccattg gtaacaattt gctatgcaat taatgttatc gcaaatagta acagaacgct
     1081 atacagaatc acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac
     1141 attgtgacga tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga
     1201 ttttgataat gaaatgc
//
LOCUS       DAJTMK010000382         1202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTMK010000382 DAJTMK010000000
VERSION     DAJTMK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.389"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..416)
                     /locus_tag="OO135_004357"
     CDS             complement(<1..416)
                     /locus_tag="OO135_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399907.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACATGIYCRAVCTKSVYSYQPDLFEDCGGA"
     gene            complement(413..796)
                     /locus_tag="OO135_004358"
     CDS             complement(413..796)
                     /locus_tag="OO135_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399908.1"
                     /translation="MYAKSFLALDGNGRLIGARTAQTAPYAHYTCHLCGSALRYHPQY
                     DTERLWFEHTDDGLTEHAQQCPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            complement(963..>1202)
                     /locus_tag="OO135_004359"
     CDS             complement(963..>1202)
                     /locus_tag="OO135_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5399909.1"
                     /translation="DYRAPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERD
                     DDTGFQQVLERADYLAKESTKAYGTGERNFGCSRG"
BASE COUNT          272 a          354 c          307 g          269 t
ORIGIN      
        1 gcacctccgc agtcctcaaa caagtctggc tggtagctgt aaacagactt cgtacatacc
       61 gctctgcaat aaatccccgt agcacaggcc tcacagaact tcttcccatg aaaaaactgc
      121 tcacacatca cacaatgcca ctccagcacg accggtaatg gtgataaaaa catcgccata
      181 tcctcaatgt aagggtggga cttttccgga ttcagcacca cacagtccgc cttctgttgc
      241 tcgctcaggg catgtaaatc gtgctcaaac catgccccct gagcatctgt ctgcagaatt
      301 aaccggccat gacaggaaag gcagaaacaa tgccggatat ttctgctgag cgcagagtcg
      361 gcactgataa tgtgctcacc cggcgtgatc cccagccccg tttttatacc gttcattctg
      421 ctaccccctc ctcactgaag cgcccggtct ggcagtgtgt gcagtaccgc tccccataat
      481 aatcgtggtg acattgtctg cagtgccagc tggctttacg caccacgggt aaggcatccg
      541 ggacgaattg ctgcagacgt ttaatcaacc gtatttctct gcgttccggc ctgacataag
      601 gacattgctg cgcgtgctcc gtcagcccgt cgtcagtgtg ttcaaaccag agccgttcgg
      661 tgtcgtattg cgggtggtat ctgagtgcac tgccgcaaag gtggcaggtg tagtgggcat
      721 aaggagcagt ctgtgcggta cgggcaccga tcagacgtcc gttgccatca agtgcgagaa
      781 aagattttgc gtacatagta tatgttcctc tgcgccagac gacacgcagg caacagccgt
      841 cccggtactg gcagcgtggt caggtgtgaa tggcggtttt cagttcagtc tcagaaggga
      901 aaatcagtgt ggtcgctgtg aacacgtcat cacactaagg tcagataagt cgtctgtcgg
      961 gctcagccac gactgcagcc aaagttgcgc tcaccggtgc cgtaagcttt ggtactctcc
     1021 ttcgccagat agtcagcacg ctccagcacc tgctgaaagc cggtgtcatc atcgcgttcc
     1081 agccacaccg ccggcctgac cggaaaatgc gccagcgtgg tatgatctcc ggtatccact
     1141 cccagggcac tgcaccacgc ctgtttaatc atcccggcca gtgatcccgg agcgcggtaa
     1201 tc
//
LOCUS       DAJTMK010000383         1194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTMK010000383 DAJTMK010000000
VERSION     DAJTMK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.390"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..750)
                     /gene="ipaH4.5"
                     /locus_tag="OO135_004360"
     CDS             complement(<1..750)
                     /gene="ipaH4.5"
                     /locus_tag="OO135_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5399910.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTN"
BASE COUNT          338 a          213 c          259 g          384 t
ORIGIN      
        1 attcgtacaa atgggcaatg tgattatatt ttcgggtaat gaagttagta ggttattgga
       61 aacatccagt gtcaatagtt cttgcggtaa tctggataat ctgcgtagtt gattttcacc
      121 taccttcaaa tattttaagc gctgaggaaa ttctggtagt tcctgtagtc tgttgccgga
      181 aacaaagagt tcttgtagtc tatgaggcaa ttcaggcaag acagtaaggc gattaccttc
      241 aatccgtaat attgttagcg ttgatggaag ctctggtaac gtctccagac tattaaaacg
      301 tgccgagagt gattgtaaat atggtggtaa tgatggcaga catgacaaat ttcgattgaa
      361 tgaaatatta agcttaatca gttgagatgg aagtgtcggg agtatattca agttgttacc
      421 atttacatga agttctgtca aaagcggaga attttcaggt agggagatta actcattgtt
      481 ggaaatatta agttcacgta tatgcaaagg gagtggtggt aaagaacgta gtttcagtaa
      541 gcttaaattg agaacaggct cccgggtatc cagacatatt ttcaataatc gaaccgcctg
      601 gatgcgattt tctgttgttg ttccttccct agcccagtca tcccagatgc ggtggtaatc
      661 tctggtacac tgttcttcta ccgataaacg agatatacat gataagccac aaagggaacg
      721 aaaaaaagaa tgattgttga tcggtttcat ttattaaccc ctcactattc atatcttggt
      781 ctattaatgc aaataaaacg agaaagtctt taaaaaaata atcctagagc tttatcggcg
      841 ttttcattcc agctggagga atatgttcct gtctttccag taaccgtcga ttgttatatc
      901 agtctaccca tgttaatgtg accagttcta ctttcatcca gtttggattg atctttatcc
      961 cctatatttt caaacaattt ttatcattta actcattact gtctcgatat tcccatagca
     1021 aacgatctgg ttggttagcc cctcaaaaaa gtcagttgca cgccgtaaat ccctgccggt
     1081 attgagcgag gatttacggg ttgttgttaa ggtccgtaat cgggtcacag tttttccgga
     1141 gtcaatccgg tctgcggttt atgcttatgc gacgtgatta tgaatggtgc agtc
//
LOCUS       DAJTMK010000384         1160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTMK010000384 DAJTMK010000000
VERSION     DAJTMK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.391"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(80..289)
                     /locus_tag="OO135_004361"
     CDS             complement(80..289)
                     /locus_tag="OO135_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR5399911.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(385..759)
                     /locus_tag="OO135_004362"
     CDS             complement(385..759)
                     /locus_tag="OO135_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCR5399912.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLRRQALELIADAESARKK"
     gene            complement(774..>1160)
                     /locus_tag="OO135_004363"
     CDS             complement(774..>1160)
                     /locus_tag="OO135_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCR5399913.1"
                     /translation="FLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLD
                     KTLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTC
                     VVTDSAEKAQSVTSVECTVTVSAAAG"
BASE COUNT          241 a          396 c          264 g          259 t
ORIGIN      
        1 attttgataa tcagttcacg taacgtcgcc attcaccttt ctccgggcgt aaaaaaaccg
       61 cctcagcggt tctcatcatt catgactgtg ctgcaaagct cagcgcgtct tccagcgccg
      121 caaacggatc cacctccggc ttatcctcat cctcgcccca gcagagcatg acgtccttca
      181 gtgcaacatt catcccctgt gccccgaaaa ccgctttcac gatctgtgca ttacggatat
      241 ccccgcgctc atcacccagc ggggatatcc tgtcgaactc catccacatc atcgcctcgc
      301 tcgcactcag gctgtggcgc agttcggata aggtgcgccc cagacggagc gcaagtcgca
      361 tcagaaagcg aatttccggg cgggctactt ttttctggcc gactctgcat cagcgatcag
      421 ttccagtgcc tgacgccgca accgggcatg taccggacca tagacggcca gcacctgctc
      481 acggtcgtcc ggagtgaaca cccgctgcag gtccgtatca cacaggacat cgcagaacag
      541 cgtcacatcc gcttccaggt tacggcgggt tttcgccacc accgacaggg tatcgtcatc
      601 ctctccatca ccattgagca cttcctgcca cagataccag gcctctgccg aaggctcccg
      661 cagcaccacg ctgacattcc cccattccgg caccttcacc gttttatgac gaaaccctga
      721 cagtctcgcc agcgccagcg ttttcagatc ctttttcatg atgacccatc cccttatccg
      781 gcagctgcgc tcactgtcac ggtgcattca acagacgtca cactctgtgc tttctctgcc
      841 gaatcggtca ccacgcaggt atatttcccc gcatcagcgg actgcgcacc tggcttactg
      901 aaggtgtctg tcgtctgccc gtcaaccggc tgaccatcct tcttccaggc gtatttatac
      961 ggcggcattc ccccgttggc actgactgac attgtcagca gcgcaccggt attcacggta
     1021 agtgtcttat ccagattttt cacaaacgcc agcggtacca caaaggacac cggtttgcct
     1081 ttcagacgca gtgagaacgt tgcagccacc acaccgttgg taccggatga ccaggtgtgc
     1141 tgacgcactt ccgccaggaa
//
LOCUS       DAJTMK010000385         1152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTMK010000385 DAJTMK010000000
VERSION     DAJTMK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.392"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            263..>1152
                     /locus_tag="OO135_004364"
     CDS             263..>1152
                     /locus_tag="OO135_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR5399914.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAESLIAKIQSETDIPIVIVHGPKGEDNARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMA"
BASE COUNT          370 a          211 c          247 g          324 t
ORIGIN      
        1 cattgccgtc gatctataaa cgaaccttat gagactacct cgacagagcg catctgcaaa
       61 tgattttatg gctgattagc ggcaacatga aaaattcgct ggaagatgaa agcgcgggag
      121 ttgctggtgt tgtttaagga ttaagcatag gacccacagg ttcgagatat gaaacataaa
      181 agtccgcata tgcacaaaat ggcaccctga tgattctgat agactgcatt gcattataac
      241 cagtagggag gcgaggttag ttatgttttt agtatcattg ttgagacgta ttgcatttag
      301 ctactacgat tataaagctt ataatttcaa tattgaaaaa acagactttg ttgtcatcca
      361 tattcccgat cagattggcg atgctatggc catctttcct gttattcggg cgcttgaatt
      421 gcataaaatt aagcatctat taattgttac gtcgacaatt aatttagaag tctttaatgt
      481 gctcaaactt gaacagacta aattaacatt agtcacaatg actatgcagg atcacgcaac
      541 attaaaagaa ataaaagatt tagcgaagaa cataacacag caatacggta cgccggatct
      601 ttgtatagag gcgatgcgta aaaagaacct gaaaacgatg ttatttatca gtcagttgaa
      661 agcaaaaacg aattttcagg ttgttggttt aaccatgaag tgctattccc ctgtgtgtaa
      721 gaacgcgtcc cggatggatc agaatctccg ggctcccgta cccatgacat gggcatttat
      781 gatgcgtgag gcgggttttc cagcagtcag gccaatatat gaattgccac taagtgagga
      841 tgtactcgat gaggtgcgcg aggaaatgcg atcgttagga tcttacattg cgcttaattt
      901 agaaggtagc tcgcaggaac gtacattttc actatcgatt gcagaaagtc taatagcaaa
      961 aattcaaagt gaaacagata taccaatagt gatcgttcat ggaccaaaag gggaagataa
     1021 cgccagggta ttagtggatt gttataataa tgtctaccgt ttatccttat caccctcgat
     1081 taaacgttca gcagcaatca taaaagatgc ttatatcgca ataactcctg acacttcaat
     1141 attacatatg gc
//
LOCUS       DAJTMK010000386         1141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTMK010000386 DAJTMK010000000
VERSION     DAJTMK010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.393"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..849
                     /locus_tag="OO135_004365"
     CDS             <1..849
                     /locus_tag="OO135_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR5399915.1"
                     /translation="WVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNF
                     HQIIMDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIA
                     TYQQYGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVA
                     KLALPSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIA
                     PWTMPTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDI
                     ANALKF"
     gene            900..1141
                     /gene="chbA"
                     /locus_tag="OO135_004366"
                     /pseudo
     CDS             900..1141
                     /gene="chbA"
                     /locus_tag="OO135_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
BASE COUNT          260 a          283 c          308 g          290 t
ORIGIN      
        1 tgggtcatta aactgcccga cagcgtacct gcttcagtat cgcgttcctt ctcggcatta
       61 attcccggct ttattattct ttccgtgatg gggattattg cctgggcgtt gaatacctgg
      121 ggcaccaact tccatcagat cattatggat accatctcaa ccccactggc atcgttgggt
      181 agcgtggtgg gctgggccta tgtgatcttt gttccactgc tctggttctt cggtattcat
      241 ggcgcgctgg cgctgaccgc actggacaac ggcattatga cgccgtgggc gctggaaaat
      301 atcgcgacct atcagcaata tggttccgtc gaagcggcgc tggcagccgg taagaccttc
      361 catatctggg ccaagccgat gctggactcc tttattttcc ttgggggcag tggtgcgact
      421 ttaggcctga tcctggctat ctttatcgcc tctcgccgtg ctgattatcg tcaggtggca
      481 aaactggcgc tgccgtccgg catcttccag attaacgaac cgattctgtt tggtctgcca
      541 attatcatga acccggtgat gtttatcccg tttgtactgg tacaaccgat tctggcggca
      601 atcaccctcg cagcgtacta catgggcatt attcctccgg tgaccaatat tgcaccgtgg
      661 accatgccaa ccggtctggg agccttcttt aacaccaacg gtagcgtcgc tgcattgctg
      721 gtcgcactct tcaaccttgg tatcgcaacg ttaatttatc tgccctttgt tgtggtggct
      781 aacaaagcgc aaaacgcgat tgataaagaa gagagcgaag aagatatcgc taacgccctg
      841 aaattttaat cgatgccggt acggttaatc gtgccggtaa gaagagagag gaacgatgta
      901 tgatggatct cgataacatt cccgatacgc aaacagaagc tgaagagctg gaagaagtgg
      961 tgatggggct tatcatcaac tccggacaag cgcgcagcct ggcgtatgcg gcactgaaac
     1021 aggcgaagta gggcgatttt gccgcagcaa aagccatgat ggatcagtca cggatggcat
     1081 tgaatgaagc gcatctggta cagacgaaac tgattgaagg cgatgcgggc gaaggtaaga
     1141 t
//
LOCUS       DAJTMK010000387         1136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTMK010000387 DAJTMK010000000
VERSION     DAJTMK010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.394"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..611
                     /locus_tag="OO135_004367"
                     /pseudo
     CDS             1..611
                     /locus_tag="OO135_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(856..>1136)
                     /locus_tag="OO135_004368"
     CDS             complement(856..>1136)
                     /locus_tag="OO135_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5399916.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          301 a          291 c          285 g          259 t
ORIGIN      
        1 attattttca acaggttgct ttcgctactt gatgcaaacg gctttattga ctggtctgcc
       61 accgcgctgg atggcagtaa tatccgggcg ctgaaatgtg ccgccggtgc tcaaaaaaac
      121 atcccgatat cgacggagat aatggcccgg gtcgctctcg cggcggtttt ggcaccaaaa
      181 tccatctggc aacagacgga agtggcctcc cgttaaatat cgtgctaagc cccggacaag
      241 ctcatgaaag ccagttcgcg caacgacttc tggacgggat tggcgttcag cgtcagaacg
      301 gcagcatgaa acgccgtgga catgcggtac tggctgataa agcgtattca gggcgcgcat
      361 tgcgcaacga gctgaaaaat aacggtataa aggcagtaat cccccgaaag tcaaatgaga
      421 aaatggcatc ggatggacgt gcacagcttg atcgtgatgc gtaccgcaat cgtaatgtcg
      481 ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt tacgacaaaa
      541 cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt tatcaacgct
      601 tacgtaatta agggacacaa cctagatcac ttagagggaa ctcagcccgg attgtgcgat
      661 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gatcaatgcc gatcatagca
      721 ccaatttccc gtgacgaacg atgcctgatg gagaaagcca tccataaaac acatgataaa
      781 tctatgttct gcgctttgat aattttgacg atgccgttga gtggtaagcg cccagagaag
      841 tacgtagcgg aaggattatt ttacagctga gaagttccag ggcagcagtt catgcactcg
      901 gttcgacggc cagtcattga gcttcacgat cacttcgcgc aaccagtcct ccggctccac
      961 tccgttcagt ttgcaggtga ccagcagact gtagataatc gccgcgcttt ctccaccgtt
     1021 gtcagaaccg aagaagagat aattttttct tccaaccgcc accgatcgca gcgcgttttc
     1081 accgatgttg ttgtcgattt ccacctggcc attacgacag tacacgttca ggctat
//
LOCUS       DAJTMK010000388         1115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTMK010000388 DAJTMK010000000
VERSION     DAJTMK010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.395"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..1038
                     /locus_tag="OO135_004369"
     CDS             <1..1038
                     /locus_tag="OO135_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5399917.1"
                     /translation="FQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTTLMGYTQWC
                     CSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTLPCQYWS
                     LVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHIHLSTTAGG
                     VTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREWNRFLDT
                     HYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLRYNSHRTKR
                     EEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVRKTAMQ
                     ITWRVRQYDFP"
BASE COUNT          267 a          257 c          346 g          245 t
ORIGIN      
        1 tttcagcagg gaaaccgctg gcttaactgg ctggagaagc agccggaagg ggcagtacgt
       61 ccggtggtga ttgagtcggt gaccaaaatc atggcgtgcg ggaccacact gatggggtat
      121 acacagtggt gctgttcatc accggactgt tgccatacaa aaaaggtctg cttccggtgt
      181 aaaagccgct cctgcccgca ctgcggggtg aaggctggcg cacagtggat acagtatctg
      241 ctgagcctgg tccccgactg cccatggcag catattgtgt tcacgttacc ctgccagtac
      301 tggtccctgg tgttccacaa ccggtggttg ctggcagaga tgagccgcat tgcagcggat
      361 gtgatactgg aaatctgcca tcaggcagat gtggagccga ggatattcac ggtgatccac
      421 acatgggggc gtgaccagca gtggcatccg catatccatt tatcgacaac tgccggtggt
      481 gtgacgtcgg gccacacctg gaaaaatctt catttttacg cccgtaaggt gatgagcatg
      541 tggcgctacc ggataacacg gttactgtca cggaaatacc ctgacctggt gatgccggat
      601 gcgctggcag cagaaggaag cagtaaacgg gaatggaatc gcttcctaga cacgcattac
      661 cgccgtggct ggaatgtcaa cgtatcccgg gtgatggata atgccaccca tgtggcggtg
      721 tactttggct cttacctgaa aaagccaccg gtgccggtga gtcgtctgga gcattatgcc
      781 ggtcaggatg aaatcggact gcgttacaac agtcaccgaa caaaacggga agaatacctg
      841 ttgatgagtg gagatgagtt catggaaagg ttctcctggc atgtggcgga taaggggttc
      901 cgtatggtga ggtactacgg ttttctgagt ccggcgaaac ggcggttact ggaagaagtg
      961 gtgtacatca taacggagac agtgagaaaa acggcgatgc aaatcacctg gagagtaagg
     1021 cagtatgact ttccgtgaga cagatgctgg ccaacgtggg atatttaacg cgggttaaca
     1081 tcgttgtcag agccgcatgg gtgagctgtt gctac
//
LOCUS       DAJTMK010000389         1107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTMK010000389 DAJTMK010000000
VERSION     DAJTMK010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.396"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(183..755)
                     /locus_tag="OO135_004370"
     CDS             complement(183..755)
                     /locus_tag="OO135_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5399918.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATITAGTANADSQFTVEYKK"
BASE COUNT          350 a          234 c          212 g          311 t
ORIGIN      
        1 ccgcgtacca ccaatgacaa cgcctgcata agttaatgct ggttggccta aaaaaacgct
       61 gagtataatc gcggtaaata acttacatga aactgttgtt ttaaagagcc tgattaaaca
      121 actttttctg gacataacga ctccctggcc aaagccaact gaataaaaag gcccttgata
      181 aattatttct tatattcgac agtaaattgt gaatcagcgt tcgccgtacc agccgttatg
      241 gtcgcattgg ttgccacata gcgtgcaata aacttcattt cagccttgcc ttcagcatcg
      301 atagcggtat aagcagagtc gttataaagt ttaacctgcg aattatcgga ctggttaaaa
      361 atacgaatac caacgccggt tgccgcaatc gcttgtcctg taccggtaaa atcaccaggg
      421 ttgcctgctg taagcggcgt acccactttc aggtcgccgt cgccgccagg cgcttctgta
      481 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      541 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      601 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
      661 ccgacgaagt gaacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
      721 agagtgaatg caccgcctat aatattacgc ttcataaaaa caccattgat ataatatatc
      781 ctttaccatg aatcatcact tatattaata agtgtatttt tgattcaaag gaatagtaca
      841 ccaaagagac ggctttatct aaaaataata ttaattacgc aaaaataaaa tataaattta
      901 ttgtttaaat attatttaaa cagtaaaaaa tcaagtttgt tatcagacat taatgataat
      961 tcagaatata tttatataga aatatagatg aatttattga tgtcagttat aaaaaagggc
     1021 caccggtcgg cagtaatgcc gatcagttaa ggatcagttg accgatccag tggctgtgta
     1081 agaatccgga aacgctcact tgtttcc
//
LOCUS       DAJTMK010000390         1099 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTMK010000390 DAJTMK010000000
VERSION     DAJTMK010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.397"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..556)
                     /locus_tag="OO135_004371"
     CDS             complement(<1..556)
                     /locus_tag="OO135_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5399919.1"
                     /translation="MLEKSLATLFVLLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNC
                     FELFLLLIPLAGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTASNDIAQY"
     gene            complement(556..>1099)
                     /locus_tag="OO135_004372"
     CDS             complement(556..>1099)
                     /locus_tag="OO135_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5399920.1"
                     /translation="YKHHVTANHITLTALALSLFTGLLLVLVAQPILFLLLPIVLFIR
                     MALNALDGMLARECNQQTRLGAILNETGDVISDIALYLPFLFLPESNASLVILMLFCT
                     ILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGLAVAIYPQWMQWNNLLWSIASI
                     LLLWTAINRCRSVLFMSAER"
BASE COUNT          389 a          266 c          223 g          221 t
ORIGIN      
        1 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
       61 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
      121 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
      181 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtatt aataacagaa
      241 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
      301 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
      361 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
      421 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
      481 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacacgaaa agtgttgcca
      541 gagatttttc cagcattatc tttcagcact cataaaaagc acactccgac aacgattaat
      601 cgcagtccag agaagcagaa ttgaagcaat gctccataaa agattattcc attgcatcca
      661 ctgtggataa atggcaacgg ctagccccca cagaccaaat attaacgcgc gatcgctttt
      721 gccgaatgga ccggcataac tgcgaatacc gttaatcgtc tgcgcgagta aaccgcaaaa
      781 ctcagtcaat atggtgcaaa ataacatgag tattacgagt gatgcgttac tttccggtaa
      841 aaataaaaac ggtaaataga gcgcaatatc ggaaataaca tcgccagttt cattcaaaat
      901 cgcccccaga cgtgtttgct gattgcactc acgcgccaac atgccatcca gcgcattgag
      961 cgccatacgg ataaaaagca cgatgggcaa tagcaaaaag aggatgggtt gtgccaccaa
     1021 caccaacagc aatccggtaa aaagagaaag cgccagtgcc gtaagagtga tgtgattcgc
     1081 tgtaacgtgg tgcttataa
//
LOCUS       DAJTMK010000391         1096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTMK010000391 DAJTMK010000000
VERSION     DAJTMK010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.398"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            204..623
                     /locus_tag="OO135_004373"
     CDS             204..623
                     /locus_tag="OO135_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5399921.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKSQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            623..1096
                     /gene="umuC"
                     /locus_tag="OO135_004374"
                     /pseudo
     CDS             623..1096
                     /gene="umuC"
                     /locus_tag="OO135_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
BASE COUNT          270 a          232 c          287 g          307 t
ORIGIN      
        1 ttctattcta tttttttgcc gggtgatatt tttcatttct gctggatgag cgtcgtcgcc
       61 agaaggccac gtgagcacag gataagagaa cgaaaaatca gcagtctata cagcgacaaa
      121 tattgatagc ctgaatcagt attgatctgc tggcaagaat agactactgt atataaaaac
      181 agtataactt caggcagatt attatgttgt ttatcaagcc tgcggatctc cgcgaaattg
      241 tgacttttcc gctatttagc gatcttgttc agtgtggctt tccttcaccg gcagcagatt
      301 acgtcgaaca gcgcatcgat ctgaatcaac tgttgatcca gcatcccagc gcgacttact
      361 tcgtcaaagc aagtggtgat tctatgattg atggtggaat tagtgacggt gatttactga
      421 ttgtcgatag cgctattacc gccagccatg gtgatattgt catcgctgct gttgacggcg
      481 agtttacggt gaaaaaatcg caactacgcc cgacggtaca gcttattccc atgaacagtg
      541 cgtactcgcc cattaccatc agtagcgaag atacgctgga tgtctttggt gtggtgatcc
      601 acgtcgttaa ggcgatgcgc tgatgtttgc tctctgtgat gtaaacgcgt tttatgccag
      661 ctgtgagacg gtgtttcgcc ctgatttatg gggtaaaccg gtggttgtgc tatcgaataa
      721 tgacggttgc gttatcgccc gaaacgctga ggcaaaggcg ctgggtgtta aaatgggcga
      781 tccctggttc aaacaaaaag atctgtttcg tcgctgtggt gtggtttgct ttagcagcaa
      841 ttatgagctt tacgcagaca tgagcaatcg ggtgatgtcg acgctggaag aactatcgcc
      901 ccgcgtcgag atttacagta ttgatgaggc attctgcgat ctgacaggtg tgcgtaattg
      961 ttgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1021 ggtgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1081 tggcagcggc agacgg
//
LOCUS       DAJTMK010000392         1093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTMK010000392 DAJTMK010000000
VERSION     DAJTMK010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.399"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..780)
                     /locus_tag="OO135_004375"
     CDS             complement(<1..780)
                     /locus_tag="OO135_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5399922.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQ"
BASE COUNT          315 a          156 c          256 g          366 t
ORIGIN      
        1 ttgcagagca tgggatgaca atgggttatc actaatatct attgaacatt cattcctcag
       61 attaagaatg ctttccggga tatgacttat ctgatttatg tcaaaataac cgttccttaa
      121 attttccggc aggtatggaa tatcggttaa catattgtcg attgcccata tggagtgaat
      181 gttgtgaggt agagggggga gttcactaag aagattccct attgcgctaa tctcttgtaa
      241 agaaaatggt aaaggtggta aaactgccag cccattacat gatacatcta atgtttccag
      301 tagttctggt aaaacaggaa gagaacataa ttgattattt gagacatgaa gctccttcaa
      361 agatatgggg agtgtgggta gtgtgattag ttgattgtgg gacgcattca ataatttaag
      421 tccttgaggc aaagcaggca gttcaataag tctgttatag ctgacattaa gctgtgtaag
      481 ggacgcaggc aatggggaga ttaagcttaa attattttta cttatattaa ttgatttaat
      541 tcccgggggg atttcaggta acgttgtcag gcctaattca gacaagtcta ggttcgtctc
      601 ttggttttgt agacatgata ctagtcgctg aaaagcgatg tctcgttgtt cttcttgtat
      661 gcggttattt ttccattcag tccaatgggt tagataattt tcatatgcgc ggctagtgtc
      721 gattgaataa gtggagaacg aatttgaaat taatctgtga ttgttatttg tcgggagcat
      781 aaatatcagg gcgtattctt tatgttgaga ggtgttggat tcttttttat ctgctttgca
      841 atgttattgg ttcccttcag cagtagacag gaacttctgg aggaggcgga tatatagttt
      901 gtgtaagcat attaactcca tatgttatat attgaagaac ttctgcttta cactatccta
      961 aactggatcg gtttaatgta aaaaaacgat actttttggg ggagggggca atttttcctt
     1021 aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg gtatcagtga
     1081 atcatgtata taa
//
LOCUS       DAJTMK010000393         1089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTMK010000393 DAJTMK010000000
VERSION     DAJTMK010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.400"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..281
                     /gene="tnpA"
                     /locus_tag="OO135_004376"
                     /pseudo
     CDS             1..281
                     /gene="tnpA"
                     /locus_tag="OO135_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          367 a          225 c          185 g          312 t
ORIGIN      
        1 atgtatgtgc tgattttgaa gctgaactgg ttgaaatgga tggcgaacca gatcacgtcc
       61 atttgttaat taactatcct cccaaactgg caatatccag tctggtaaac agcctcaaag
      121 gcgtatcggg taggtgactg cgacgagatc taccagatat tgcagtcagg tattactaca
      181 aaggcgtttt gtggagtcct ggctattttg ccagtagctg cggaggtgcg ccaatatccg
      241 tcatccgcca atacattgaa caacagcaaa cacctggtta ggtggaaaac cacgccttat
      301 atccctgccc tgaaggacgg ggttttacgg cgcaccggat aagcctggag attatctaca
      361 ccagcatgga catcattgta ctatgccccg tacatgcccc atcacatcct caacatcaaa
      421 gtattagagc cttgcaaacc attatgcgtc tcagtttgtc tcatctatct tacgcaaaaa
      481 aaaatagaac taatatcaag cggcgctata ttgcgtatta aataaaacca cctcagttac
      541 attaataaat ctaaacaaac atcatgcaaa taatttccat ggttatttct agggtattta
      601 aattaaataa ataaattatg agtcaatcat aaataacaac ctttaatata atttaactac
      661 caaatttata ttcgttcaca aaatagtcat gcattgtgtg caatagaaac agttcagata
      721 aagataggga tctacttcac aatttaaatg agaaactaaa acttacatct tgaaataatc
      781 tcattgattg gatgaatatt tgtagtgcag tgacatcatt tttaaataat agttcaaata
      841 aaagggctca cgatgaaaaa attaacagtg gcaatttctg ctgtagctgc atcttaacgt
      901 ataaactaga tttaataaca acgatatccg tacctatccg gatatctaat gtcataagca
      961 tccctttaat tatcccctca acctcttgta actttcccac tcttgaactc cacatagtcc
     1021 aaacgccgga tgcaaaaaaa tcacacgtgc atttctgaaa ttgtgatgga ggaagatagt
     1081 atcaattat
//
LOCUS       DAJTMK010000394         1085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTMK010000394 DAJTMK010000000
VERSION     DAJTMK010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.401"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            312..>1085
                     /gene="kgtP"
                     /locus_tag="OO135_004377"
     CDS             312..>1085
                     /gene="kgtP"
                     /locus_tag="OO135_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5399923.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLC"
BASE COUNT          262 a          255 c          262 g          306 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aaatttatcc ttaagctcct caataaccat
      181 tttcctgcta actaaattca tggttaatgt tgcataataa tacgcaacaa atgtatcaca
      241 ttttctttac caaaagaaat aaacaaaagc gaccaacaaa agcatcggat tacggcagga
      301 gacataatgg catggctgaa agtactgtaa cggcagacag caaactgaca agtagtgata
      361 ctcgtcgccg catttgggcg attgtggggg cctcttcagg taatctggtc gagtggttcg
      421 atttctatgt ctactcgttc tgttcactct acttcgccca cattttcttc ccttccggga
      481 acacgacgac tcaactacta caaacagcag gtgtttttgc tgcgggattc ctgatgcgcc
      541 caataggcgg ttggctattt ggccgcatag ccgataaaca tggtcgcaaa aaatcgatgc
      601 tgttatcggt gtgtatgatg tgtttcggat cgctggttat cgcctgcctc cctggttatg
      661 aaactatagg tacgtgggct ccggcattat tgcttctcgc gcgtttattt cagggattat
      721 ccgttggcgg agaatatggc accagcgcca cctatatgag tgaagttgcc gttgaagggc
      781 gcaaaggttt ttacgcatca tttcagtatg tgacgttgat cggcggacaa ctgctagccc
      841 tactggttgt cgtggtttta caacacacca tggaagacgc tgcactcaga gagtggggat
      901 ggcgtattcc tttcgcgtta ggagctgtgt tagctgttgt ggcgttgtgg ttacgtcgtc
      961 aattagatga aacttcgcaa caagaaacgc gcgctttaaa agaagctgga tctctgaaag
     1021 gattatggcg caatcgccgt gcattcatca tggttctcgg ttttaccgct gcgggctccc
     1081 tttgt
//
LOCUS       DAJTMK010000395         1078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTMK010000395 DAJTMK010000000
VERSION     DAJTMK010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.402"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(440..990)
                     /locus_tag="OO135_004378"
                     /pseudo
     CDS             complement(440..990)
                     /locus_tag="OO135_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          212 a          286 c          283 g          297 t
ORIGIN      
        1 tacatgcgga ataatcccgt atctacctgc gcggcgctgg cttcttccac tggtaggcag
       61 gtgctcgcat catttcgcgg aacctgtcgc gccgggctat atcggcatta actgagcgtt
      121 ctatctcccg ttgatctgcc tgtgtaagct ggtggcctcc atgctcaagt ttggcgatgg
      181 tcaataaggc tatttcgcgt ggtgtcatac gtcataccct ctggaaggat gaaacacgcc
      241 cggattaaat ttcatttttc gcgggtatga aaaaacgatg gggcgggcag tccttaaggc
      301 tcaggattgc agagatttgg cctccccctc ccggtgatag tttattgctt gatgtggacg
      361 gcttgctata ttggcggcga atgcaaattg tcgcagaagt ctatcccgtt ctctgacgcc
      421 ccgtgcgggc gttttgcttt tatagagtca ggccaatgcc tctggctgct tttcctgctt
      481 tacgctcaat aacgcccgtg attctgtcca ctaccgggct gaatgcctgc cagtaagcca
      541 tctccataac tgataactct gcccgcttcc tgtcaaacgt agcccagcgc atcccctttg
      601 gttttgggaa catgcgaatg tcattaaaca gattattggc aaggtcattg ctgccccata
      661 tcgcgcatcg ctgctggcgt atcttcatgc gcatcctgtc cagtatgtcg gcactctggc
      721 ttttatagtg aagttacagt gggggcaagc gtaccattcc cggacgccat acccggcctt
      781 cgtggttgtg gtgcgaatag cccgcgtcac gccgttgatt gtcatggcgt agctgtcggc
      841 ttgccggaag gcatacaacg ttccgctttc ggtctgggta taaagctgcg tacccggttc
      901 agcatccgcc agcctgctac gcatgttcgc aaggaatggc aaccctatac ggggcaggtc
      961 ggtggtatag ctgcgcgttc tgtttctcac tggctggcct cctgcgaatt actgcgcatt
     1021 aactgcgaac ggtgcgcaaa ttgaatacga aataatatgc gtattttact gtaaattg
//
LOCUS       DAJTMK010000396         1045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTMK010000396 DAJTMK010000000
VERSION     DAJTMK010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.403"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            6..851
                     /locus_tag="OO135_004379"
     CDS             6..851
                     /locus_tag="OO135_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5399924.1"
                     /translation="MEHLLKSESTALRESIYAVIAGKDCYQADTLSLLPGVKIISRFG
                     TGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGE
                     SHGYELQGKRIGMVGYGNIGKTLAKRLSGFDVELLAYDKQPDYQVADKVGVQFVSIED
                     IFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERN
                     VFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGTHVAQSIIDYFAGRE
                     IKNVL"
     gene            complement(930..1006)
                     /locus_tag="OO135_004380"
     tRNA            complement(930..1006)
                     /locus_tag="OO135_004380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(970..972),aa:Met,seq:cat)
BASE COUNT          285 a          211 c          254 g          295 t
ORIGIN      
        1 ataatgtgga gcatctgctt aaatcagaat ccactgcatt acgagaatct atttacgcgg
       61 tcatcgccgg aaaagattgt tatcaggcgg acacgctatc attgctgcct ggtgtgaaaa
      121 taatatcgag attcgggacg gggatagata atattgatct tcgtgcagca caacaatccg
      181 gtattgtagt caataatgcg gttgggatta attcaaatgc tgtcgcggaa tttatcattg
      241 gacttatctt cgcgagcatg agaaatattc ccggcagcta tcatgcgatg caaaatggct
      301 actggggtga gtcgcatggc tatgaattac aaggcaaacg gattggcatg gttggctacg
      361 gcaatatagg taaaaccctg gcaaaaaggt tatccggttt tgatgttgag cttttagctt
      421 atgacaaaca acccgattat caggttgctg acaaagttgg agttcagttt gtatcaattg
      481 aagatatctt tatgcagtcg catgtcatca ttgttctttt gcctttttct cctgagctgg
      541 agaacttcat tagtcataaa tatttgtcga tgatgcgcaa tggtgctcta atcatcaatg
      601 ccgcaagggg aaagttgctg gatgagggcg ctttactaca ggtgattgaa gaacgaaatg
      661 tgtttgcggc gctggatgtg tttagctctg aaccgttggc gcaatttagc ccactgctac
      721 atgccaaaaa tatcattaca acgccgcata ttgcggccgc caccgttgag tcataccagc
      781 aaaccggtac acacgttgcc cagtcgataa ttgattactt cgcaggaagg gagataaaaa
      841 acgtgttgtg agttcagcga acgaagtttt taaatacaaa aaaaacgcaa tttgtggaac
      901 acattgcggt ttatcagcat tctaactatt ggttgcgggg gccggatttg aaccgacgac
      961 cttcgggtta tgagcccgac gagctaccag gctgctccac cccgcgtcac cgtactgctt
     1021 tatttcatca aatttgattg gttgc
//
LOCUS       DAJTMK010000397         1032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTMK010000397 DAJTMK010000000
VERSION     DAJTMK010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.404"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..588
                     /locus_tag="OO135_004381"
     CDS             <1..588
                     /locus_tag="OO135_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HCR5399925.1"
                     /translation="TITSGGNPPAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINK
                     NCRVLGKLSANQIEGDLVKTVGKPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPA
                     VAFRGAKHERKNNNIYSSCRLIVKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLE
                     FSVSAWLVNGWYPTASISDLLVVVMKKATAGITIS"
     gene            656..>1032
                     /locus_tag="OO135_004382"
     CDS             656..>1032
                     /locus_tag="OO135_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5399926.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVST"
BASE COUNT          254 a          244 c          292 g          242 t
ORIGIN      
        1 accataacca gcggcggtaa tcctccggca ttttccctga cacctggcgg acggctgacg
       61 gcgaaaaatg ccgatatcag cggtaacgtg aacgcgaact ccgggacgct caacaacgtc
      121 acgattaata agaactgtcg ggttctggga aaattgtccg cgaaccagat tgaaggcgat
      181 ctcgttaaaa cagtgggcaa acctttccca cgggactccc gggcaccgga gaggtggcca
      241 tcagggacca ttaccgtcag ggtttatgac gatcagccgt ttgatcggca aattgttatt
      301 cccgcggtgg cgtttcgcgg tgctaaacat gagcggaaga ataacaatat ttattcgtca
      361 tgccgcctga tagtgaagaa aaacggtgct gaaatttata accgaacgac cctggataat
      421 acgctgatat atacgggtgt tattgatatg cctgccggtc acggtcacat gacgctggag
      481 ttttctgtat cggcatggct ggtaaatggc tggtatccca cagcaagtat cagcgatttg
      541 ctggttgttg tgatgaagaa agccactgca ggcatcacga ttagctgaat tttataaccc
      601 agatacgggc accagaaatg gtgccttttt tattgcagaa aagcgagagg taattatgcg
      661 taaagtttgt gcagccattt tgtccgcagc catctgtctg gctgtatccg gtgcgcctgc
      721 atgggcgtct gaacatcagt ccacgctgag cgccgggtat cttcagcccc atactgatat
      781 gccaggcagc gatgacctga agggcattaa cgtgaaatac cgttatgagt ttacggacac
      841 gctggggctg gtgacgtcat tcagctatgc caacgctgaa gatgagcaaa aaacgcatta
      901 cagcgatacc cgctggcatg aggattccgt gcgtaaccgc tggttcagcg tgatggcggg
      961 gccgtctgtg cgcgtgaatg aatggttcag cgcgtatgcg atggcgggcg tggcttacag
     1021 ccgtgtgtcg ac
//
LOCUS       DAJTMK010000398          987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTMK010000398 DAJTMK010000000
VERSION     DAJTMK010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.405"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..135
                     /locus_tag="OO135_004383"
     CDS             <1..135
                     /locus_tag="OO135_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="biosynthetic; catalyzes the formation of trehalose
                     and phosphate from trehalose-6-phosphate; expression is
                     increased under osmotic stress and induced during the
                     transition to stationary phase and by decreased
                     temperature; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5399927.1"
                     /translation="ATQASWRLAGVPDVWSRLEMITTALQQKRENNRSDDYESFSRSI
                     "
     gene            110..>987
                     /locus_tag="OO135_004384"
     CDS             110..>987
                     /locus_tag="OO135_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5399928.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLMQFQRPA
                     GDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     NEALLEKYPQHHGKIR"
BASE COUNT          266 a          241 c          246 g          234 t
ORIGIN      
        1 gcaactcagg catcatggcg actggcgggt gtgccggatg tctggagcag gcttgaaatg
       61 ataaccaccg cattacaaca aaaaagagaa aataacagga gtgatgacta tgagtcgttt
      121 agtcgtagta tctaaccgga ttgcaccacc agacgagcac gccgccagtg ccggtggcct
      181 tgccgttggc atactggggg cactgaaagc cgcaggcgga ctgtggtttg gctggagtgg
      241 tgaaacaggg aatgaggatc agccgctaaa aaaggtgaaa aaaggtaaca ttacgtgggc
      301 ctcttttaac ctcagcgaac aggaccttga cgaatactac aaccaattct ccaatgccgt
      361 tctctggccc gcttttcatt atcggctcga tctgatgcaa tttcagcgtc ctgccgggga
      421 cggctatcta cgcgtaaatg cgttgctggc agataaatta ctgccgctgt tgcaagacga
      481 tgacattatc tggatccacg attatcacct gttgcccttt gcgcatgaat tacgcaaacg
      541 tggggtgaat aatcgcattg gtttctttct gcatattcct ttcccgacac cggaaatctt
      601 caacgcgctg ccgacatatg acaccttgct tgaacagctt tgtgattatg atttgctggg
      661 tttccagaca gaaaacgatc gtctggcgtt cctggattgt ctttctaacc tgacccgcgt
      721 cacgacacgt agcgcaaaaa gccatacagc ctggggcaaa gcatttcgaa cagaagtcta
      781 cccgatcggc attgaaccga aagaaatagc caaacaggct gccgggccac tgccgccaaa
      841 actggcgcaa cttaaagcgg aactgaaaaa cgtacaaaat atcttttctg tcgaacggct
      901 ggattattcc aaaggtttgc cagagcgttt tctcgccaat gaagcgttgc tggaaaaata
      961 tccgcagcat catggtaaaa ttcgtta
//
LOCUS       DAJTMK010000399          982 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTMK010000399 DAJTMK010000000
VERSION     DAJTMK010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.406"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..288)
                     /locus_tag="OO135_004385"
     CDS             complement(<1..288)
                     /locus_tag="OO135_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399929.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARR"
     gene            complement(239..982)
                     /locus_tag="OO135_004386"
                     /pseudo
     CDS             complement(239..982)
                     /locus_tag="OO135_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          217 a          268 c          251 g          246 t
ORIGIN      
        1 acgtctggcc tctctggccg cttccagcat ttctggtgtc acagtgaatg gggctatctc
       61 accccatttg ccgctttgca gttcctgcca gatttgctga cccgtcggag cgacatcatc
      121 agcggtggct gtgtagggga ctgcctggtc cctgtcgtca aaaaaaacgt cacagtctac
      181 tgcgccactt tcggtataac ggggattaat gattttttta atttccacgg tgcattcctc
      241 acgatgtgcg aataaaaagc ccgggcattg cgccagagac atgtgcatcc ggcaccccgg
      301 acagggcgca atatgacccc ggtaatgaat gctctgaaca tcccgtaatg aaaaattgtg
      361 gggatgctat atacgttccg gtgggagtac tgggcactga aatccccacc ggtcccagtc
      421 gtgagcctct gtatgactgc cccctgacaa gtctgatgac tttatcaccg tcagcttctc
      481 cctggtacgc agcaataatc agcccgccaa tgtcagggtc tccccatctg ttgcggacag
      541 agctcgccac gattctgtaa ataatatctt ctgtggttat atttattttc acccagccag
      601 tcagtctgga tatgggccag tagcagtagc gggtgtgata aatggggccg ttaatgccgt
      661 aaaaagtaag ggatttggct ctgtaccgcg gttctgttgt ctcagggcgt gcatcagtcc
      721 accggatgct gagcatcccc ttcaaaccgt gtgtcgggta tgatgatgtc gtaggggcca
      781 gcaacggaat attcacctgg cagcgcattc cttacccagg ccaggaaatc actcttagtg
      841 tcaaaacgga taacatcttc aggcagaaaa gcacacccaa agccgaatgc gccgggtatc
      901 gccagacggc cttttgtccg gtcgtaaatg tcgctctgtg cttccatcgt ggccgcactt
      961 ttcagcccca gattatcccg gg
//
LOCUS       DAJTMK010000400          981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTMK010000400 DAJTMK010000000
VERSION     DAJTMK010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.407"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>981
                     /locus_tag="OO135_004387"
     CDS             <1..>981
                     /locus_tag="OO135_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5399930.1"
                     /translation="HPETEALLWQHETRHAYSAQGLANRCIPDSLPAVEWLTYGSGWL
                     AGMKLGDTPLVDFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWN
                     DNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPD
                     STLSMWPDNRIARDAHYLYRYDRHGRLTEKTDLIPEGGIRTDDERTHRYHYDSQHRLV
                     HYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRL
                     TTIQNDRTRIQTVYQPGSFTPLIRVETATGELAKTQRRSLADALQQSGGED"
BASE COUNT          224 a          329 c          289 g          139 t
ORIGIN      
        1 cacccggaga cggaagcact gctctggcag catgagacca gacatgcgta cagcgcgcag
       61 ggactggcga accgctgtat accggacagc ctgcccgccg tggaatggct gacctacggc
      121 agcggctggc tggcaggtat gaagctcggc gacacaccgc tggtggattt cacccgcgac
      181 cgcctgcacc gggaaacgct gcgccgcttc ggccgttatg aactctccac cgcttacacc
      241 cctgccgggc agttacagag ccagcacctg aacagcctgc agtatgaccg cgattacacc
      301 tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc
      361 tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaacct tgatatccgc
      421 atcccgtatg ccacagaccc ggcaggtaac cgcctgcccg acccggagct gcacccggac
      481 agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta tctttaccgg
      541 tatgaccgtc acggcaggct gacagagaaa accgacctca tcccggaagg gggtatccgc
      601 acggatgatg agcgcaccca ccggtaccat tacgacagtc agcaccggct ggtgcactac
      661 acgcggacac aatatgcaga gccgctggtc gaaagtcgct atctttacga cccgctgggc
      721 cgcagggtgg caaaacgggt atggcggcgt gaacgggacc tgacgggctg gatgtcgctg
      781 tcacggaaac cggaagtgac ctggtacggc tgggacggcg accggctgac cacaatacag
      841 aacgacagaa cccgcatcca gacggtttat cagccgggaa gcttcacgcc actcatcaga
      901 gttgaaaccg ccaccggtga gctggcgaaa acgcagcgcc gcagcctggc ggatgccctt
      961 cagcagtccg gcggcgaaga c
//
LOCUS       DAJTMK010000401          969 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTMK010000401 DAJTMK010000000
VERSION     DAJTMK010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.408"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            8..>969
                     /locus_tag="OO135_004388"
     CDS             8..>969
                     /locus_tag="OO135_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCR5399931.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLKSFLLVHGVHY
                     VGRADWGPAHRRWLSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRL
                     YPVVEALQAMRGIQFITAVGLISELGDLTRFEHPRQLMSWFGITPSEYSSGGSRHQGS
                     ITKAGNSYARKLLVEAAWSYRHPARISPAIQKRQENLPRPVIDRA"
BASE COUNT          245 a          233 c          259 g          232 t
ORIGIN      
        1 ggccagcatg aacaacaata acacattgta cgtcgggtta gatgttcaca aagaatcgat
       61 tactgtcgct tatgctatca attcagaacc tgttgaactg atgggtaaaa ttggcacatc
      121 acctactgat attcagaatc tttgtaaacg tctcaggtca aagtcatcgc aggtcagtat
      181 cgtttatgaa gcggggccct gtggttatgg actttatcgt cggctggtga aatccggttt
      241 tgactgcatg gtctgcgccc cctcacttat cccgaaaaaa ccgggggagc gggttaaaac
      301 cgatcgccgt gatgccatca gacttgtgcg ttcactgcgt gcaggagatc tttctgctgt
      361 ctacgtaccc ggcattgaag atgaggcatt ccgggatttg gcccgggcat gggcatctgc
      421 ccgcgatgat ttacggcatg caaggcaacg tctgaaatcg tttcttctgg ttcatggggt
      481 ccattatgtc gggcgagcag attggggccc tgcacaccga cgctggctca gcaaatactc
      541 attcgaaagt ccctggcgac aattagcttt tgacgaacac cgtcgtacaa tagaagaccg
      601 gcaggcacaa tgtgaacggc tggaatccgc cctgaaggag gccgttaccg aatggaggct
      661 gtatccggta gtagaggcgc tgcaagccat gcgagggatt cagtttatta ctgcggttgg
      721 attaatttct gaactgggtg atctgacccg ttttgaacat ccacgacaac tgatgtcatg
      781 gtttggtatc acgccatcag aatattcatc gggtggaagc agacatcagg gcagcattac
      841 caaagccgga aacagctatg ccagaaagct attggtcgag gcagcgtgga gttaccgtca
      901 tccggcacgc atcagtccgg caatacaaaa aaggcaggaa aatttacccc gccccgtcat
      961 tgacagagc
//
LOCUS       DAJTMK010000402          948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTMK010000402 DAJTMK010000000
VERSION     DAJTMK010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.409"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            231..902
                     /gene="queE"
                     /locus_tag="OO135_004389"
     CDS             231..902
                     /gene="queE"
                     /locus_tag="OO135_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5399932.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWHLSMQTHK
                     YLNIA"
BASE COUNT          268 a          197 c          226 g          257 t
ORIGIN      
        1 ttatcattaa aaactaatac agaaaaaata aatataattt tggtgaatgt tttgacatga
       61 ttaaatccat tttctttatt ttcttctgac ataatacgtt actcccttta attattaacg
      121 atatgtttcg catttttctt taaatctgca tgtaacagaa tgttaataag acatgattgt
      181 tgcggcagga tctgcaataa ttaacaatta tccacttcac agagaatgct atgcagtacc
      241 cgattaacga gatgttccag accctgcaag gtgagggtta ctttaccggc gtccccgcca
      301 tttttattcg tttacaggga tgcccggttg gctgtgcctg gtgcgacacc aaacacacct
      361 gggaaaagct tgaggatcgg gaagtctccc ttttcagcat tctggcgaag accaaagaga
      421 gtgataagtg gggggcggcg agcagtgaag atttgctggc tgtcattggt cggcagggat
      481 ataccgcgcg gcatgtggtg attacgggtg gtgagccttg cattcatgat ttgctgccac
      541 tgactgacct gctcgaaaag aacggtttta gctgccagat cgaaaccagt ggaactcatg
      601 aagtacgctg cacaccgaat acctgggtta ccgtatcgcc aaagctgaac atgcgcggcg
      661 gctatgacgt gttgtcacag gcactggagc gagccaacga aatcaagcat ccagtggggc
      721 gcgtacgcga tattgaagca ctggatgaac tattggcaac gctgaccgat gataaaccgc
      781 gagtcattgc actgcagccg attagccaaa aggatgatgc cacacgtttg tgcattgaaa
      841 cctgcattgc gcgtaattgg catttgtcga tgcaaacaca taaatatcta aatattgcct
      901 gattaaacat ttataagcgt tataaatggg tggaacctgt aaagactt
//
LOCUS       DAJTMK010000403          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTMK010000403 DAJTMK010000000
VERSION     DAJTMK010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.410"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            7..558
                     /gene="ybcL"
                     /locus_tag="OO135_004390"
     CDS             7..558
                     /gene="ybcL"
                     /locus_tag="OO135_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5399933.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            568..>902
                     /locus_tag="OO135_004391"
     CDS             568..>902
                     /locus_tag="OO135_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5399934.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQ"
BASE COUNT          276 a          170 c          198 g          258 t
ORIGIN      
        1 gtttttatga aaaaacttat cgtttcaagt gtattggcat tcataacatt ttctgcgcag
       61 gccgcagcat ttcaggtcac tagtaatgaa ataaaaacag gagagcaact tacaacgtct
      121 catgtatttt ctggatttgg gtgtgagggg ggtaatacat cgccctcatt aacctggtct
      181 ggtgctccag aaggcaccaa aagctttgcc gtaactgtgt atgatccaga tgcgcctaca
      241 ggcagcggat ggtggcactg gactgttgct aatattccag caacggtaac atatttgccc
      301 gctgatgcag gaagacgtga tggtacaaaa cttccgactg gtgctgtcca aggccgaaat
      361 gattttggct atgcgggatt tggtggagca tgtcctccca aaggagataa accccatcat
      421 taccagttta aaatatgggc tctaaaaact gataagattc ctgtggattc taattccagc
      481 ggagcgttag ttggttatat gcttaatgcc aataaaattg caaccgctga gataataccc
      541 gtttatgagg taaagtagga taagagtatg ctggcaaaag ataggactaa cctaaagatt
      601 gaagaaatac ggatgcataa acatcatgag attcacaggg ttaagcctct tatgccagct
      661 ttatgtcgta tccgtcaggg aaaaaaagtt atcaattggg agacgcatag tctaactgtt
      721 gataataatc agataatatt atttccctgt ggatatgaat tttatattgc gaattaccca
      781 gaagcaggtc tttatcttgc agaaatgctt tattatccca ttgatctaat tgaaaagttc
      841 caaaatcttt atgcgataac cgatcaaatt cgtaacacga caggtttctg cttacctcag
      901 aa
//
LOCUS       DAJTMK010000404          887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTMK010000404 DAJTMK010000000
VERSION     DAJTMK010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.411"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..680)
                     /locus_tag="OO135_004392"
     CDS             complement(<1..680)
                     /locus_tag="OO135_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5399935.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLNDINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          268 a          143 c          183 g          293 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tatcattaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggatagaggg
      661 ggattattta caggaagcat aataacctcg cagaaggata tcctgataaa atgtgatact
      721 gggaaataat aagctaaaat aaaatgtttc aggattgaaa tttattgttt tgattttaag
      781 gaagtgatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttca
//
LOCUS       DAJTMK010000405          884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTMK010000405 DAJTMK010000000
VERSION     DAJTMK010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.412"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            12..>884
                     /gene="pgrR"
                     /locus_tag="OO135_004393"
     CDS             12..>884
                     /gene="pgrR"
                     /locus_tag="OO135_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5399936.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDNQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFID"
BASE COUNT          227 a          194 c          220 g          243 t
ORIGIN      
        1 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
       61 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
      121 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
      181 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
      241 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
      301 gtattactac tgtagaacat gcagcaaaaa cgatattgtt accagcaatg cgcacattcc
      361 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
      421 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
      481 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
      541 gccgaagtgt tccaacgtca gtgtcacaat taatagataa tcaggcaatt aatttgtatc
      601 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
      661 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
      721 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
      781 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
      841 cacaccgtcg acatgctggc tcggcattct cattatttat agat
//
LOCUS       DAJTMK010000406          883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTMK010000406 DAJTMK010000000
VERSION     DAJTMK010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.413"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>883
                     /gene="ag43"
                     /locus_tag="OO135_004394"
     CDS             <1..>883
                     /gene="ag43"
                     /locus_tag="OO135_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5399937.1"
                     /translation="GGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKEG
                     GLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVENGKAD
                     GVVLESGGRLDVLEGHSAWKTLVDDGGTLAVSAGGKATSVTITSGGALIADSGATVEG
                     TNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGSLSGRT
                     QLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAVLSRAVAKGDSPVTFHKLTTSN
                     LTGQGGTINMRVRLDGSN"
BASE COUNT          210 a          223 c          281 g          169 t
ORIGIN      
        1 cggcggcgac cagacggtac acgggcatgc gctggatacc acactgaatg gcggttacca
       61 gtatgtgcac aacggaggca cagcctctga cacggttgta aacagtgacg gctggcagat
      121 tgtcaaggaa ggtggtctgg cggatttcac caccgttaac cagaaaggca aactgcaggt
      181 gaacgccggt ggtacagcca cgaatgtcac cctgaagcag ggaggcgcac tggtcaccag
      241 tacggcggca accgtcaccg gcagcaaccg tctgggcaat ttcactgtgg aaaacggtaa
      301 ggctgacggt gttgttctgg agtccggtgg tcgcctggat gtactggagg gccattcagc
      361 ctggaaaaca ctggtggatg acggcggaac cctggcagtg tctgccggtg gtaaggcgac
      421 aagtgtcacc ataacatccg gtggtgccct gattgcagac agtggtgcca ctgttgaggg
      481 gaccaatgcc agcggtaagt tcagtattga tggcacatcc ggtcaggcca gcggcctgct
      541 gctggaaaat ggcggcagct ttacggttaa tgccggagga ctggccagca acaccactgt
      601 cggacatcgt ggaacgctga cgctggctgc cgggggaagt ctgagtggca gaacacagct
      661 cagtaaaggt gccagcatgg tactgaatgg cgatgtggtc agtaccggcg atattgttaa
      721 cgcaggggag attcgctttg ataatcagac aacaccggat gccgtactga gccgtgctgt
      781 tgcaaaaggc gactccccgg taacgttcca taaactgaca accagtaacc tcaccggtca
      841 gggtggcacc atcaatatgc gtgttcgcct tgatggcagc aat
//
LOCUS       DAJTMK010000407          866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTMK010000407 DAJTMK010000000
VERSION     DAJTMK010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.414"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..565
                     /locus_tag="OO135_004395"
     CDS             <1..565
                     /locus_tag="OO135_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399938.1"
                     /translation="KAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQPDTQTAEQDFL
                     TRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVRPKEPNWTAWA
                     NEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELSLRLSPSVSTH
                     TEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            562..>866
                     /locus_tag="OO135_004396"
     CDS             562..>866
                     /locus_tag="OO135_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399939.1"
                     /translation="MSMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPGPQSVRETRPVRNFYVGTNGPRVILCLTRQA
                     "
BASE COUNT          217 a          229 c          242 g          178 t
ORIGIN      
        1 aaaagcgggt tttcacccgt cagaatctgg cggggatccg tcagtaaaat caaaacatga
       61 tccgtcagat aaaaaacctt ctcgtccgga cgcttcgcaa ccggacacgc agacggctga
      121 acaggatttt ttaactcgcc atcctgatgc ggttgtattc agccctaaaa agcgccagtg
      181 ggggacgcag gatgatttga cctgcgcaca gtggctctgg aaaaaaatca tcgccctgta
      241 cgagcaggct gccgaatgtg acggcgaggt ggttcgtccc aaagaaccga actggacagc
      301 ctgggcaaac gaaattcgcc tgatgtgtgt acaggatggc cgtactcaca aacaaatctg
      361 cgagatgtac agccgcgtca gccgcgatcc gttctggtgc cgtaacgtgc tcagcccgtc
      421 gaagttgcgg gaaaaatggg atgagctttc cctgcgctta tcgccgtccg tcagcacgca
      481 cacagaaaaa cgtgaagacc cgtacttcaa agccagttac gacaacgtgg actacagcca
      541 gatcccggca ggattcaggg ggtgagcatg agtcttttga atgacgttca gaaattcatt
      601 gaagcccatc cggggtgtac ttccggagac attgcggatg cttttgcagg ttactcacgg
      661 cagcgcgttc tgcagtctgc aagcaagtta cgtcagagtg ggcgtgtggc tcaccgttgt
      721 gaaggagata cacgcagaca tttcccgcgc ctgactgaga gagcgcagga accgggacca
      781 caatcagttc gagaaaccag acctgtgcgc aatttctatg tcggcactaa cggtccccgg
      841 gtgattttgt gcctgacccg ccaggc
//
LOCUS       DAJTMK010000408          865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTMK010000408 DAJTMK010000000
VERSION     DAJTMK010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.415"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..426
                     /locus_tag="OO135_004397"
     CDS             <1..426
                     /locus_tag="OO135_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5399940.1"
                     /translation="GAENIFLGRKAATKEEAIRFAGEQLVKGGYVEPEYVQAMLDREK
                     LTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEEDDIARLVIGIAARNN
                     EHIQVITSLTNALDDESVIERLAHTTSVDEVLELLAGRK"
     gene            656..>865
                     /locus_tag="OO135_004398"
     CDS             656..>865
                     /locus_tag="OO135_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5399941.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIG"
BASE COUNT          203 a          208 c          253 g          201 t
ORIGIN      
        1 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
       61 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
      121 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
      181 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
      241 gtgcgcttcg gtgaagagga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
      301 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
      361 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgtt ggcaggtcgt
      421 aagtaatcca atcccaccct ctccacatgg agaaggtggg gttaattgcc tgatgcgcta
      481 cgcttatcag gcctacagga tgcatcacga tttgttgaat ttgcacgttc ttgtaggccg
      541 gataaggcgc ttacgccgca tccggcgctg cccctctcct cacggagagg gtttgggtga
      601 gggaaaagcc tcaccccagc cctctcgggt aaaaacattg atgaaggtta atactatgaa
      661 agcattacat tttggcgcag gtaatatcgg tcgtggcttt atcggtaaac tgctggcaga
      721 cgcgggtatc caactgacgt ttgccgatgt caatcaggtg gtacttgatg ccctgaatgc
      781 ccgtcatagc tatcaggttc atgtggttgg tgaaaccgag caggtagata ccgtttccgg
      841 cgtcaatgct gtcagcagca ttggt
//
LOCUS       DAJTMK010000409          857 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTMK010000409 DAJTMK010000000
VERSION     DAJTMK010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.416"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>857
                     /gene="tuf"
                     /locus_tag="OO135_004399"
     CDS             <1..>857
                     /gene="tuf"
                     /locus_tag="OO135_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5399942.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTL"
BASE COUNT          202 a          212 c          245 g          198 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccct
//
LOCUS       DAJTMK010000410          850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTMK010000410 DAJTMK010000000
VERSION     DAJTMK010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.417"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..188)
                     /locus_tag="OO135_004400"
     CDS             complement(<1..188)
                     /locus_tag="OO135_004400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399943.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDA"
     gene            273..602
                     /locus_tag="OO135_004401"
                     /pseudo
     CDS             273..602
                     /locus_tag="OO135_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            632..715
                     /locus_tag="OO135_004402"
                     /pseudo
     CDS             632..715
                     /locus_tag="OO135_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            715..810
                     /locus_tag="OO135_004403"
                     /pseudo
     CDS             715..810
                     /locus_tag="OO135_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          242 a          192 c          201 g          215 t
ORIGIN      
        1 gcatccacaa agaacggaat attcaggtct gcgcagcatt caacggcatc gtcaaaacta
       61 tcaaagcgca gaacttctgc gtcttcttcg tcaaaaaaat catcttcgtg aagcttcacg
      121 acatagcggg gaagtttgct tctttgagag gcgggtttac gtttacgggg tttagctgaa
      181 cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg gtaattgcag
      241 accatgacaa gccgcagccg tcaggctgcc tactcgagct tcagctcaat aaaggcgagt
      301 atcgacataa attaccccga tggatctttt ttgcaaatca gggtgaattc acgaccggtg
      361 attacgaaga aatcatgaat aaggccagcg ccctgagtct ggtatcgaat gcaatcctgt
      421 actggaacac atcaagaata agcaatattg ttgaagggtt acgtgaaaat ggtgaggtta
      481 ttgatgatga cgtattagcc catatatcat tactgcccta taaacacgta ttacctaatg
      541 gtacttattt tgttgatgga gaaccttacg gtactgagcc taccgaaaga gcaaaaccgt
      601 aagtttctgt ttaaatgctc ccgctggtcc atccggacag attatcgacc atcaggctgt
      661 acaggcctgg gcggacagcc tcagtactga caatccgtta ccggtgccac gctgatggaa
      721 cagaagtgga ccagtaaggc gctttctgat ttggcgcggt tatatgattt tctggtgctg
      781 gccagtaaac ctgctgccgc cagaatggta cacgtatagg aaattgaaaa accagacgat
      841 accgctgtcc
//
LOCUS       DAJTMK010000411          848 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTMK010000411 DAJTMK010000000
VERSION     DAJTMK010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.418"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..800)
                     /gene="wecH"
                     /locus_tag="OO135_004404"
     CDS             complement(<1..800)
                     /gene="wecH"
                     /locus_tag="OO135_004404"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5399944.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHAL"
BASE COUNT          262 a          208 c          194 g          184 t
ORIGIN      
        1 atcaaggcgt ggaatccgta tatacccaat gaatggcggg agattaagcc aagtccgcga
       61 atggtacgcg tatccagcgt gtttttaacc agagtcaata gcgcgattgc gcagataaaa
      121 accatcggcc cacagtaaag ataccaggta tcggcaaaat ttccgcgcca ctgcaattca
      181 tataatgtcc cgcgagagat aataaaaacc cccgtcgcaa acagcgcggc gctcacccac
      241 gacagtgctt tatgctgtgt gtccatcatc cctatagcgc ggcccaacat gccatacaga
      301 atgtagtaaa aagtatcgcc attgatatat aagttaattg gcagccattc aaaaccgtca
      361 attttctgcg gcactgtgtt tgggttagcg ataatgccaa tcaccgccat tagcaccagc
      421 aacatttttc cgccgacgtt cttcacctga atcagcggtg aaaccagata aatcaccgca
      481 atcgcgaaga aaaaccacaa gtggtaaaac actggctttt gcagcaggtt tttcagcgct
      541 aactccatat tgatggaggt aaacagcgca atgtagagca gtgcgattgc gctataaaaa
      601 atcagacata agccgatacg caagaaatgg cgcggctggg cgctgcgttc gccaaaaaag
      661 agatagccgg aaatcatgaa aaatagcggc acgctgacac gagaggcaga attcagaaca
      721 ttggcgatat cccatgtgac ggggctaaca ctatgagcat tggtcacata ccaggtagtg
      781 gtgtgaatca tcaccaccat taaacacgct atccctcgca ggttatcaat ccagtaaatt
      841 ttgggctg
//
LOCUS       DAJTMK010000412          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTMK010000412 DAJTMK010000000
VERSION     DAJTMK010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.419"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            34..842
                     /locus_tag="OO135_004405"
                     /pseudo
     CDS             34..842
                     /locus_tag="OO135_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
BASE COUNT          194 a          213 c          218 g          217 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcagtt tactggtggg ctacatcgcc
       61 tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa tcaatctggg cctggcgcaa
      121 catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca atgtattggt
      181 gtagagattt tgctgcgctg gaacaatccg cgtcagggcc ggatttcacc ggatgtgttt
      241 attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt gatggcagaa
      301 accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg cattaacgtc
      361 gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta ctggtttagc
      421 gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt actggatgtt
      481 gattatcgga ttgcccgcga gctacatcgt aaaaacgtca aactggcgat tgatgacttc
      541 ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt gctgaaaatt
      601 gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt gaccgatatc
      661 atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt ggaaacgcaa
      721 gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta tttgtacgca
      781 cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc gccgcccgcc
      841 cg
//
LOCUS       DAJTMK010000413          842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTMK010000413 DAJTMK010000000
VERSION     DAJTMK010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.420"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..424
                     /gene="hipA"
                     /locus_tag="OO135_004406"
     CDS             <1..424
                     /gene="hipA"
                     /locus_tag="OO135_004406"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCR5399945.1"
                     /translation="FQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDIISAFPVLGGTG
                     IHISDLKLAMGLNASKGKKMAIDKIYPRHFLATATVLRFPEVQMHEILSDFARMIPAA
                     LDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            684..776
                     /locus_tag="OO135_004407"
                     /pseudo
     CDS             684..776
                     /locus_tag="OO135_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          217 a          182 c          217 g          226 t
ORIGIN      
        1 cttccagtgg ttgattggcg caacggatgg ccatgcaaaa aacttctccg tatttattca
       61 ggctggcggc agttatcgac tcacgccatt ttacgacatc atttcagcat ttccggtcct
      121 tggcggtacg ggaatacaca tcagcgatct caaactggca atggggctta acgcatccaa
      181 aggcaaaaaa atggcaatcg ataaaattta tccgcgacat tttttggcga cagcaacggt
      241 gctgagattc ccggaagtgc agatgcatga aatcctaagt gactttgcca gaatgattcc
      301 ggcagcactg gataacgtga agacttcatt accgacagat tttccggaga acgtggtgac
      361 ggcagttgaa accaatgtgt tgaggttgca tggacggtta agccgagaat acggtagtaa
      421 gtgagatatg gggtttggtc gttgttaata agcaaccatg accgccaacg actggattct
      481 cccttggttt atgttggtgc gcataatgta tattatgtta aatcatatct gagtgatgcg
      541 aagtgctttt ctggtagtcg ttattcgttc agggttccag aacttaagcc tctcctcttt
      601 cataattggc ctgtgcatga cggatatagg ctggctggta tgcagatttg ccgtttgccg
      661 catcttccca gcgttgaaga ctcaagctgg caaaccgcgt gcaaatgagc acgccctctt
      721 tccatcaaca cttcaggcag cttgcgggaa tgagtccact acggtatcag aaatgggaag
      781 tgggtgtctc tctgatgcgt aatggaaaaa tactggctac gggagaaaat gtctccctag
      841 gg
//
LOCUS       DAJTMK010000414          820 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTMK010000414 DAJTMK010000000
VERSION     DAJTMK010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.421"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>820
                     /gene="dsdA"
                     /locus_tag="OO135_004408"
     CDS             <1..>820
                     /gene="dsdA"
                     /locus_tag="OO135_004408"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5399946.1"
                     /translation="GTTSLAEGLPYVGLTEQDVQDAHARLSRFAPYLAKAFAETAATG
                     GIIESELVAIPAMQKRLEKEYQQPISGQLLLKKDSHLPISSSIKARGGIYEVLAHAEK
                     LALEAGLLTLDDDYSKLLSPEFKQFFSQYSIAVGSTGNLGLSIGIMSARIGFKVTVHM
                     SADARAWKKAKLRSHGVTVVEYEQDYGVAVEEGRKAAQSDPNCFFIDDENSRTLFLGY
                     SVAGQRLKAQFAQQGRIVDADNPLFVYLPCGVGGGPGGVAFGLKLAFGDHVHCF"
BASE COUNT          192 a          194 c          220 g          214 t
ORIGIN      
        1 ctggcacgac ctcattggct gaaggtttac cttatgttgg cctgaccgaa caggatgttc
       61 aggacgccca tgcgcgctta tcccgttttg caccctatct ggcaaaagca tttgctgaaa
      121 ctgctgccac tggggggatt attgaatcag aactggttgc cattccagct atgcaaaaac
      181 ggctggaaaa agaatatcag caaccgatca gcgggcaact gttactgaaa aaagatagcc
      241 atttgcccat ttccagctcc ataaaagcac gcggcgggat ttatgaagtc ctggcacacg
      301 cagaaaaact ggctctggaa gcggggttgc tgacgcttga tgatgactac agcaaactgc
      361 tttctccgga gtttaaacag ttctttagcc aatacagcat tgctgtgggc tcaaccggaa
      421 atctggggtt atcaatcggc attatgagcg cccgcattgg ctttaaggtg acagttcata
      481 tgtctgctga tgcccgggca tggaaaaaag cgaaactgcg cagccatggc gttacggtcg
      541 tggaatatga gcaagattat ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg
      601 acccgaactg tttctttatt gatgacgaaa attcccgcac gttgttcctt gggtattccg
      661 tcgctggcca gcgtcttaaa gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata
      721 accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg
      781 ggcttaaact ggcgtttggc gatcatgttc actgcttttt
//
LOCUS       DAJTMK010000415          819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTMK010000415 DAJTMK010000000
VERSION     DAJTMK010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.422"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..536)
                     /locus_tag="OO135_004409"
     CDS             complement(<1..536)
                     /locus_tag="OO135_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5399947.1"
                     /translation="MPQLKGVIKTPTGEPLDGATITLTSIHNRAGILKSVFSHVTTQN
                     GEYDFPVLPGVYSVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESLKK
                     FEELAQQAQQSAGSAAGNARQTAQDVTAAATARDDAQRFAEKARQDATVTAENRKATA
                     EDVTSTGANAAAAGQSAQ"
     gene            complement(523..>819)
                     /locus_tag="OO135_004410"
     CDS             complement(523..>819)
                     /locus_tag="OO135_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5399948.1"
                     /translation="ANRRITAPGYWFYRNDEFVLDYRLKAEDERDALLAQVSARTGEW
                     EEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKATYNAINWPERPDAAA"
BASE COUNT          144 a          233 c          216 g          226 t
ORIGIN      
        1 tcctgtgcgc tctgtccggc tgcggctgca tttgctcctg tgcttgtcac atcttccgca
       61 gtggcctttc tgttctcagc tgtgacggtt gcatcctgtc gggctttctc cgcaaaacgt
      121 tgtgcatcat cacgggccgt tgcggctgcc gtcacatcct gcgccgtctg ccgtgcgttt
      181 ccggctgcgc tcccggcact ctgctgcgcc tgttgcgcca gctcttcgaa tttcttcagg
      241 gattccggac gcaggtcaat attagttgcg cccagaaaat cattcagcga accgtccgtt
      301 gaatcttcat aaacgcggat gacaccgatt tctgaaaggc gccgtgtgct ttgtgtcagg
      361 cgaacgctgt acacacccgg cagaacgggg aagtcatatt ccccgttctg tgtggtgaca
      421 tggctgaaaa cacttttcag aatccctgca cggttgtgta tggaggttag tgtaatggtt
      481 gcgccgtcca gcggttctcc cgtgggcgtt ttaatcacac ctttaagctg cggcatcggg
      541 acgctccggc cagttgatgg cgttatatgt ggctttatct gtgatggtgc tgaaatccat
      601 cgcctgcagc gatttcgcgt aaatacgaca ggctttcagc ttttctttat cttcgtcgct
      661 gattaacccc agcaacaggt cttcttccca ttcccctgtc cgggcactga cctgagccag
      721 aagggcatca cgctcatctt ccgctttgag tctgtagtca aggacaaatt catcattgcg
      781 gtaaaaccag taacccggcg cggtaatgcg gcggttggc
//
LOCUS       DAJTMK010000416          803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTMK010000416 DAJTMK010000000
VERSION     DAJTMK010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.423"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>803
                     /locus_tag="OO135_004411"
     CDS             <1..>803
                     /locus_tag="OO135_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HCR5399949.1"
                     /translation="RSLLSGWVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLP
                     GNKKTKTGRLWTYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYA
                     GFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTA
                     EQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWA
                     EADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGVEP"
BASE COUNT          183 a          214 c          261 g          145 t
ORIGIN      
        1 cgttcactgc tgtcgggctg ggtggatgca tgctgccggc tactgtcacc gctggaagaa
       61 gcgcttcagg actatgtgct gactgacggt aagctccatg ctgatgacac gcctgtcccg
      121 gtgctgttgc caggcaataa gaaaacgaag accgggcggt tatggaccta cgttcgtgac
      181 gaccgtaacg ccgggtcaac gctggcgccg gcggtgtggt tcgcttacag cccggacaga
      241 aaaggcatcc atccgcagac ccatcttgcg gggttcagtg gtgtactgca ggcggatgca
      301 tacgccgggt tcaacgagct gtaccgggat ggccggataa cggaagccgc ctgttgggct
      361 cacgcccgcc gtaaaatcca cgatgtgcac gttcgcaccc cgtcagccct gacggaggaa
      421 gcgctgaaac ggatcggcga actgtacgcc atcgaggcag agataagggg aatgacggcg
      481 gagcagcgcc ttgccgaacg tcagttgaaa acgaaaccgc tgctgaaatc cctggaaagc
      541 tggctgcgtg aaaagatgaa aaccctgtcg cgacactcag aactggcgaa agcgttcgca
      601 tacgccctga accagtggcc ggcgctgacg tactatgcag atgatggctg ggctgaggcg
      661 gacaataaca tcgctgaaaa tgcgttgcgg atggtcagtc tgggccgcaa aaactacctg
      721 ttcttcggtt cggatcatgg aggagagcgg ggagcgctgc tgtacagcct gatcgggacg
      781 tgcaaactga acggagtgga gcc
//
LOCUS       DAJTMK010000417          777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTMK010000417 DAJTMK010000000
VERSION     DAJTMK010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.424"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(47..383)
                     /locus_tag="OO135_004412"
                     /pseudo
     CDS             complement(47..383)
                     /locus_tag="OO135_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          250 a          142 c          141 g          244 t
ORIGIN      
        1 taaatatgaa aagatggttt aaataccccg ttacttttga cttacactat acggtatcgc
       61 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
      121 cgtacgacca cagtattctg gctcctgtgt ggtcatgctt taattctgcg ttccggacag
      181 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
      241 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
      301 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
      361 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
      421 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
      481 tgtttctgac gaaatcaaca tgaaaagaac catatatgat aattattata ataacggttt
      541 taattggaat acatatatta caacgtatta tatataattg gtattctagg aactatattc
      601 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
      661 agaattgata aaaaaacgcc agcaacacat agtgccggcg gagggaatac cccatggaga
      721 aaatgtgatg cctagagcac catgaatata taattaaaaa tagttagcgt tgtcaat
//
LOCUS       DAJTMK010000418          739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTMK010000418 DAJTMK010000000
VERSION     DAJTMK010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.425"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..87)
                     /locus_tag="OO135_004413"
     CDS             complement(<1..87)
                     /locus_tag="OO135_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5399950.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNA"
     gene            403..>739
                     /locus_tag="OO135_004414"
     CDS             403..>739
                     /locus_tag="OO135_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5399951.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQP"
BASE COUNT          191 a          155 c          184 g          209 t
ORIGIN      
        1 ggcattgtcg aaattcaggg tgtattctgg agtgacaaca atgtcctgtt cttcaggcac
       61 cagcttaaag caggttatta ttttcatgat ttctcctgtg agtcgcatac ggaaccatct
      121 ccgatggaag gtaaaaatgg cctgaaaata gtgtgcgata gcggtgcggg gttttcgacg
      181 gagcaagctg ttttccttgc caacaatctt gcaaaaaaaa tgcgatcgct aaaacaaaag
      241 tgcaatttgc ggcaatatag ttattccatg accgtacaat cttgcattca gtgtaaataa
      301 tcttgtttag ctctcgctaa acgcggttgt ataaccgcaa tacacgccgt attttatccg
      361 gcatatattg tttttgcttg ctcatggaat agggaggttg tcatgtatca acgctgtttt
      421 gataacgcca gtgaaacgct gtttgtcgcc ggtaaaacgc cacggctttc acgttttgca
      481 tttagcgatg atccaaaatg ggagtccgga catcacgttc atgacaatga aaccgagttg
      541 atttacgtca agaaaggggt tgcaaggttt accatcgatt cttcgttata tgtcgcacat
      601 gcggatgaca ttgtggtgat agaacgcggc aggctgcatg cggtggcctc tgacgttaac
      661 gatccggcaa cgacgtgtac ctgtgcgctg tacggctttc agtttcaggg ggctgaggaa
      721 aatcagctac tgcaaccgc
//
LOCUS       DAJTMK010000419          728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTMK010000419 DAJTMK010000000
VERSION     DAJTMK010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.426"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
BASE COUNT          285 a          137 c           80 g          226 t
ORIGIN      
        1 aaaaagttaa gaattaacat gggtaggccg tttaaagagc ctacccactg acttatttat
       61 ctttttttga aaaaaattaa gataagaaaa agaacaatat ttgttacagg aaataccccc
      121 aaaactcccc agtacttact ccatcctaga gacatcgtat atctaccaag gatttgacaa
      181 aacccaaaac ttaaccctat acctatactt cttaagttta atgtcgtagt caggcttaaa
      241 ataaatagaa caaggatggt aatagcccac cacaataacc atgccttaac aaaatcagat
      301 tttttgctgt aaggttattt acccaagaag aactgccaaa agttaccatt tcccatatag
      361 aatccattca cccaagatat tgtttataga ataaaaatac atttcaatta tatttaatta
      421 aaatataccc catcaataaa aacttacaca ctctaaatta ctactgtata gttatcaatg
      481 caaagactgc tatattgcat atatatatgc aaaccacata attcaggtaa tatatattca
      541 aaacaattat atatataaaa caatgtaaaa atgttcaaat catccatcag aaatatattc
      601 tacccatcca ggcttgagcg tttatcacgc tttcagttca taatctatac attaacatca
      661 tggtcaatat taataacttt gaatgatatc aacctggctt tgagttttat tctggttatt
      721 aataatac
//
LOCUS       DAJTMK010000420          709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTMK010000420 DAJTMK010000000
VERSION     DAJTMK010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.427"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..612
                     /locus_tag="OO135_004415"
     CDS             <1..612
                     /locus_tag="OO135_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5399952.1"
                     /translation="KGVQAAIIKADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEA
                     GKLMGKLLNGKGTVAIISYPALQSVVARVDGFKKGLSDTPDIKIVAEQPSITRAEALT
                     TAQNIMQANANLNGLFGFGDDAALAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKT
                     FAAVIRQYPDQMGAKAIDAAVDHLNGKPVEKMIPVQPGVYTGK"
BASE COUNT          193 a          147 c          187 g          182 t
ORIGIN      
        1 aaaggcgtcc aggcggcgat aattaaagcc gataaggccg gtattcccgt tatcaccgtc
       61 gacgtcgctg cggaaggcgt tccggtcgta tcgcatgttg cgaccgataa ctatgcggga
      121 ggcgttgaag caggcaagtt aatgggcaaa cttttgaatg gtaaaggtac tgtagccatt
      181 atttcttatc cggcactaca atcggtggtt gcgcgcgttg atggttttaa gaaagggtta
      241 agcgatacac cggatattaa aattgttgct gaacagccta gtattacgcg ggctgaagcg
      301 ttaacgactg cgcaaaacat tatgcaggca aatgccaacc tgaatggtct gtttggcttt
      361 ggtgatgatg cggcgctggc agcggttatc gcagcaaaat ctgcgcataa cgacaacatc
      421 aaaatcattg gttttgacgg catgaaagaa gcgcgtgatg ccgttgacag tgaaaaaacg
      481 tttgcggccg tgattcggca atatcccgat caaatgggtg ctaaagccat tgatgccgct
      541 gttgatcacc tcaatggtaa accggtagaa aaaatgatcc cggtacaacc cggcgtttat
      601 acaggtaaat agtaaacatc ttcacctcac aacaaaacgt tattggcgtt ttgttgtgac
      661 accgaggtca ttatgacgga tgttattctt gatgtttcgc atattgcga
//
LOCUS       DAJTMK010000421          701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTMK010000421 DAJTMK010000000
VERSION     DAJTMK010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.428"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            26..701
                     /locus_tag="OO135_004416"
                     /pseudo
     CDS             26..701
                     /locus_tag="OO135_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          176 a          168 c          196 g          161 t
ORIGIN      
        1 cagacaacca gtatcactta aataagtgat agtcttaata ctagttttta gactagtcat
       61 tggagtacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagctt agtcagccgt tgtctccggg tgtcgcgtgc gcagttgcac
      481 gtcattctca gacgaaccga tgactggatg gatggccgcc gcagtcgtca cactgatgat
      541 acggatgtgc ttctccgtat acaccatgtt atcggagagc tgcccacgta tggttatcgt
      601 cgggtatggg cgctgcttcg cagacaggca gaacttgatg gtatgcctgc gatcaatgcc
      661 aaacgtgttt accggatcat gcgccagaat gcgctgttgc t
//
LOCUS       DAJTMK010000422          665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTMK010000422 DAJTMK010000000
VERSION     DAJTMK010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.430"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..>665)
                     /locus_tag="OO135_004417"
     CDS             complement(<1..>665)
                     /locus_tag="OO135_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5399953.1"
                     /translation="RSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNS
                     PQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITG
                     VTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAV
                     WLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHT
                     GRP"
BASE COUNT          109 a          235 c          204 g          117 t
ORIGIN      
        1 ccggccggcc cgtgtgacgg tgcgccacca tccggccccg gtatttatca tcgtaagtaa
       61 agctacgcac ctgtgtattg ctgcggtcat acactgccgc cagttcgccg cgcggcgtcc
      121 agccatagcg caccagcggc gcggcaggta aattctccgg gtattccggg tcgtgcgtca
      181 gccacacggc agacagacgg atgccgttgt cccggccata ttcggtgtaa cccggcaggg
      241 tatcaggaaa agcggacggt tccgtcccgc cggaaatggc ctgctgccgg gcttcttctg
      301 cccgctgcgc ctgcgtggtc agtaccagtt ggaagtgacg cccggcacca tccgtcacgc
      361 cggtgatttc gccgctgaat tcaccggcgg cttcgcggtg gaacgtctgt gtgcgcccga
      421 agcggtccac cagcccggtc agtacccggt acggcggcag cggcgcaggc agcacctcat
      481 ccgcttccgg cacccgctca caccagccga gcagccacca cggcccctgc ggactgtttg
      541 tcgccagata acgatgcgga cttaagcgga gttcttccgg cagcgcctgc cagagtgcgg
      601 ccagccggtg accttcatcc agtttcgcca cgccgccgcg caccagccac agtgactcgc
      661 tgcgg
//
LOCUS       DAJTMK010000423          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTMK010000423 DAJTMK010000000
VERSION     DAJTMK010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.432"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..512)
                     /locus_tag="OO135_004418"
     CDS             complement(<1..512)
                     /locus_tag="OO135_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5399954.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRKA
                     TAEDVTSTGA"
     gene            complement(576..>655)
                     /locus_tag="OO135_004419"
     CDS             complement(576..>655)
                     /locus_tag="OO135_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5399955.1"
                     /translation="DLAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          136 a          221 c          143 g          155 t
ORIGIN      
        1 tttgctcctg tgcttgtcac atcttccgca gtggcctttc tgtcctcagc tgtgacggtt
       61 gcatcctgtc gggctttctc cgcaaaacgt tgtgcatcat cacgggccgt tgcggctgcc
      121 gccacatcct gcgccgtctg ctgtgcgttt ccggctgcag ccccgctctg ctgcgcctgc
      181 gccaccattt cctcaaagcg cttcatcacc tccggacgta aatcaccatc ctttggtgca
      241 ttcagaaacg cgttcagtgt ccccggtgca tcagtcggtg ccacatcaat atccccgaca
      301 cgggttggct gccagccgtt acagtgaagc gcaacctcgt aataccccgg ctcagcctca
      361 atcacataag caccgttatt gtccgtcacg caagtggcaa caacgtgtgc cacaacggtc
      421 ggactggttc ttctggcccg cagttcaatc gcacaattta cgacaggttt acccgcccca
      481 tctttcagta cacctgaaat ctttactgcc atattcaccc cacaaaaagc ccgcctgaac
      541 cagcgggctg tcataacact gtgttacctg gctaatcaga acttataacc gacacccacg
      601 atgaaaccgt cagtgcgcca gtcgccactg ccggaacctt cataagcaag gtcaa
//
LOCUS       DAJTMK010000424          653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTMK010000424 DAJTMK010000000
VERSION     DAJTMK010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.433"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>653
                     /locus_tag="OO135_004420"
     CDS             <1..>653
                     /locus_tag="OO135_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5399956.1"
                     /translation="VAQMKNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAE
                     YDEWGNLLNEENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQ
                     GEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSL
                     "
BASE COUNT          207 a          124 c          170 g          152 t
ORIGIN      
        1 gtggcgcaga tgaaaaacca gatggacccg gtgtacacgc cggcgcgaaa aattcacctg
       61 taccactgcg accatcgcgg cctgccgctg gtgcttatca gcacggaagg ggcaacagaa
      121 tggtgcgcag aatacgatga gtggggcaac ctgctgaatg aagagaaccc gcatcatctg
      181 cagcagctca ttcgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg cgatatatca ctcaggatcc gattgggctg
      301 aaggggggat ggaattttta tcagtatccg ctgagcccgg tgaatagcat ggatccatta
      361 ggattatatg aatttaaatc aaaaaatata gatgatattg gaatatttgc attggcaatg
      421 tgtaatggag aatcaattaa cgagaataaa gaatatggtg gactaatatg taagaagcaa
      481 ggtgaatatt tccccatgaa tccgataagt tcaaatgata atgatagtgt agacttgcga
      541 aatataaaat gccctgaagg ttcagagaga gtaggcgatt atcacactca cggtttttac
      601 tctgacgata aagggaataa agtaacaaaa gaaaatgatg tttatgatag tct
//
LOCUS       DAJTMK010000425          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTMK010000425 DAJTMK010000000
VERSION     DAJTMK010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.434"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..75
                     /locus_tag="OO135_004421"
     CDS             <1..75
                     /locus_tag="OO135_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399957.1"
                     /translation="DGLRFAIREGGRTVGAGVVAKVLG"
     gene            258..413
                     /gene="bfd"
                     /locus_tag="OO135_004422"
     CDS             258..413
                     /gene="bfd"
                     /locus_tag="OO135_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5399958.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREGSA"
     gene            397..582
                     /locus_tag="OO135_004423"
                     /pseudo
     CDS             397..582
                     /locus_tag="OO135_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="iron storage protein; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
     gene            complement(579..>647)
                     /locus_tag="OO135_004424"
     CDS             complement(579..>647)
                     /locus_tag="OO135_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5399959.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          176 a          142 c          152 g          177 t
ORIGIN      
        1 gacggtctgc gtttcgcaat ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct
       61 aaagttctgg gctaattaca cgttaattag ttttgaattg aaaagggcgc ttcggcgccc
      121 tttttgctgc ccgtaatttg agctcgaaat atttagtcgt aattttgctc gctgaactat
      181 ggcgcaaata aaactgtgct attgtaatca taaccattct catttacact ttgcgcagaa
      241 tttgaacggg agcaatcatg tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc
      301 gccaggcagt ccgtcagttt catccgcaat ctttccaaca attgcgtaag tttattcctg
      361 tgggaaatca atgcggtaag tgcattcgcg ccgcgcgcga aggatctgcg tgaggctatc
      421 gcatatgccg acagcgttca tgactatgtt agccgcgata tgatgatcga gatccttgtt
      481 gatgaagaag gtcatatcga ctggctcgaa actgaactgg atctcatcgg taaaattggc
      541 ctacaaaatt acctgcaatc acaaattaaa gttaaagatt aacggcctat cagcaaacta
      601 tcccagccta taacgaaacc cgcagccgcc agaaatggcc caaaagg
//
LOCUS       DAJTMK010000426          642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTMK010000426 DAJTMK010000000
VERSION     DAJTMK010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.435"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(177..>642)
                     /locus_tag="OO135_004425"
     CDS             complement(177..>642)
                     /locus_tag="OO135_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5399960.1"
                     /translation="PELIYCWEQLKTSIISRGFSTQIQEHLAMGVLLSLGAHHVNCLL
                     LSDSKQSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDV
                     RLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          185 a          131 c          121 g          205 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc aatgtgctta
       61 taaacgtccc cgattagcgc agaagtcacc ggattgttca ggctccaata ggttgagtat
      121 gccgtattta ttttccaaaa taaaaatcga tgctgttgct ccttcataaa atacttttac
      181 tctctggacg cttttcttag ctctcttggt gttatcttaa aacgattatg gaacctttca
      241 gtaaaacgag aaggacactt gtaaccattt tccctggcaa tctcgctgat gggttttact
      301 gtcgtttgta tagcagacaa cgcattattt aacctcacgt cgtccaatat actttgaaaa
      361 cttactccct cgcttgccag acggcgatgc aatgtggaaa cagaaatgta gagatatctc
      421 gctaccttgt ttgctgtcca ttttgttcca ggttcggata gcattaggtt ataacaacga
      481 cttattaatg attgtttact atctgataaa agtaaacaat taacatgatg tgctcctaat
      541 gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg ttgagaagcc tcgggaaata
      601 atagatgttt ttagttgctc ccaacaatat attaactcgg ga
//
LOCUS       DAJTMK010000427          634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTMK010000427 DAJTMK010000000
VERSION     DAJTMK010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.436"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>634
                     /locus_tag="OO135_004426"
     CDS             <1..>634
                     /locus_tag="OO135_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HCR5399961.1"
                     /translation="DDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINML
                     KQADVLAENFRPGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQA
                     MSGIMMETGYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDA
                     TLSFLEHGLMAYIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKL"
BASE COUNT          173 a          129 c          156 g          176 t
ORIGIN      
        1 gtgatgatac ccgcacattt ggtccctatg tggatggaca gtcactctat tacagtttta
       61 ttaatcatgg caaagagagt gtggttcttg atttaaagaa tgattacgat aaaagtatat
      121 ttataaatat gctcaaacaa gctgatgtat tagctgagaa ttttcgccca ggtacaatgg
      181 aaaaactggg gttttcatgg gaaacgcttc aagaaatcaa cccgcgcctc atatatgctt
      241 catcgtcagg tttcggacat accggtccgc taaaagatgc tcctgcctac gataccatca
      301 ttcaggcaat gagcgggata atgatggaaa caggatatcc tgatgctccg ccagtgcgcg
      361 ttggtacatc tcttgcggat ctatgcggcg gtgtctattt attcagcgga atagtgagtg
      421 cactttatgg ccgcgaaaag agccagagag gggcgcatgt cgatatagcg atgtttgatg
      481 ccacgctgag ttttctggag catggtctga tggcatatat cgcgacaggg aagtcaccac
      541 aacgtctggg aaatcgccat ccctacatgg caccttttga tgttttcaat actcaggata
      601 agccgattac gatttgttgt ggtaatgaca agct
//
LOCUS       DAJTMK010000428          634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTMK010000428 DAJTMK010000000
VERSION     DAJTMK010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.437"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            33..437
                     /locus_tag="OO135_004427"
                     /pseudo
     CDS             33..437
                     /locus_tag="OO135_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
BASE COUNT          169 a          152 c          192 g          121 t
ORIGIN      
        1 tcttcctgat ttaatttatc gcgagaaaaa ctatgtctgt agtgatatca ggtgcgctga
       61 ctgatggcgc aggtatcccc atgtccggat accatattat tctgaaatcc cgggtaaaca
      121 ccccggaagt ggtgatgcac actgttgctg atgtgatgac aggaaacgat ggtgaatact
      181 gtttccatgc gcggactgga aaatatggtg tgtatctgaa accgggctgg cacaacgagt
      241 acaacgttgg cgacattgct gtatatgagg actcaaagcc cggcacgctg aatgactttc
      301 tgattgctcc tgatgagggc gacctgaaac cggatgtcgt caaacgcttt gaggaaatgg
      361 tggtgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag acggcgcagg
      421 atgtggcggc agccgcaacg gcccgtgatg atgcacaacg ttttgcggag aatgccagac
      481 aggatgcaac cgtcacagct gaggacagaa aggccactgc ggaagatgtg acaagcacag
      541 gagcaaatgc agccgcagcc ggacagagcg cacaggatgc cgcaggttat gcccgcgcag
      601 cagaacaggc caaaaatgac attgatgctg cgct
//
LOCUS       DAJTMK010000429          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTMK010000429 DAJTMK010000000
VERSION     DAJTMK010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.438"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>628
                     /locus_tag="OO135_004428"
     CDS             <1..>628
                     /locus_tag="OO135_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5399962.1"
                     /translation="HQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQ
                     MLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYS
                     VQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGV
                     TLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENR"
BASE COUNT          171 a          147 c          177 g          133 t
ORIGIN      
        1 ccatcaaact tattggggaa cgagtaatgt cgatgagcaa ttccaggctg gattaaatac
       61 tgcgttcgaa gatatcaact ggacgctcag ctatagcctg acgaaaaacg cctggcaaaa
      121 aggacgggat cagatgttag cgcttaacgt caatattcct ttcagccact ggctgcgttc
      181 tgacagtaaa tctcagtggc gacatgccag tgccagctac agcatgtcac acgatctcaa
      241 cggtcggatg accaatctgg ctggtgtata cggtacgttg ctggaagaca acaacctcag
      301 ctatagcgtg caaaccggct atgccggggg aggcgatggt aatagcggaa gtacaggcta
      361 cgccacgctg aattatcgcg gtggttacgg caatgccaat atcggttaca gccatagcga
      421 tgatattaag cagctctatt acggagtcag cggtggggta ctggctcatg ccaatggcgt
      481 aacgctgggg cagccgttaa acgatacggt ggtacttgtt aaagcgcctg gcgcaaaaga
      541 tgcaaaagtc gaaaaccaga cgggggtgcg taccgactgg cgcggttatg ccgtgctgcc
      601 ttatgccacc gaatatcggg aaaacaga
//
LOCUS       DAJTMK010000430          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTMK010000430 DAJTMK010000000
VERSION     DAJTMK010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.439"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>628
                     /locus_tag="OO135_004429"
     CDS             <1..>628
                     /locus_tag="OO135_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5399963.1"
                     /translation="RSRGKCAGVAVVLSLAAVTSVPALAADTVVQAGETVSGGTLTNH
                     DNQIVLGTANGMTISTGLEYGPDNEANTGGQWVQDGGTASNTTISSGGLQFVGAGGKA
                     TDTIINEGGGQSLKGLALNTTLNGGEQWMHEGAIATGTVINDKGWQVVKPGAVATDTV
                     VNTGAEGGPDAENGDTGQFVRGNAVRTTINKNGRQIVAAEGTANTTVVY"
BASE COUNT          168 a          148 c          202 g          110 t
ORIGIN      
        1 cgctcacggg gaaaatgcgc cggtgtggcg gttgtgctgt ctcttgctgc tgtcacatca
       61 gtcccggcac tggctgctga cacggtcgta caggcgggag aaaccgtgag cggcggaaca
      121 ctgacaaatc atgacaacca gattgttctc ggtacggcca acggaatgac catcagtacc
      181 gggctggagt atgggccgga taacgaggcc aataccggcg ggcaatgggt acaggatggc
      241 ggcacagcca gtaataccac catcagcagc ggcgggctcc agtttgtagg tgccggggga
      301 aaggctacag acacaataat caatgaagga ggcggacaaa gtctgaaggg actggcactg
      361 aacaccacgc tgaatggtgg cgagcagtgg atgcatgaag gtgcgatagc cacaggaacc
      421 gtcattaatg ataagggctg gcaggtcgtc aaacccggcg cagtggcaac agacaccgtt
      481 gtgaataccg gcgcagaagg aggaccggat gcagaaaatg gtgataccgg gcagtttgtt
      541 cgcggaaatg ccgtacgtac caccatcaat aaaaatggtc gtcagattgt ggctgctgaa
      601 ggaacagcaa ataccactgt ggtttatg
//
LOCUS       DAJTMK010000431          606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTMK010000431 DAJTMK010000000
VERSION     DAJTMK010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.440"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            364..>606
                     /locus_tag="OO135_004430"
     CDS             364..>606
                     /locus_tag="OO135_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5399964.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKEL"
BASE COUNT          146 a          163 c          128 g          169 t
ORIGIN      
        1 tccagaacca tacggactgc ccgttgacgg acttcggggg aaaaacgagt atttttagtc
       61 atcctgttta cctctttctc aggaagttta gtctccagga ttcccggggc ggttcactct
      121 atcaatgtca gattctcttc gcccccgcag gattcgtgcc tcttactcta tgactctatg
      181 gagtttaagc ttgatcttat tgaaaaatct taccagctag gggcctgcgt ggcccaactg
      241 gccagagagt acgggattaa tgtaagcgcc ccatcagcga cgtaagcgcc ccatcagcga
      301 cgtcttgtga aaattgtcct gtctggcaac aatcacgccc atctatattg atggacacga
      361 acgatgaatt cccagacaac aaaagatatt ccctgcttcc gttcttattt gcctgatgcc
      421 ctgcgtttaa gatttgaaga taaactgacc atccgggcca tcgctcagcg tctgggtctc
      481 agtcattcca caatacatac gctttttcag cgatttcttg catccggtat cgcatggcca
      541 ttgcccgatt cagtttcatt cgctcaactt gacgccatcc tttatgccaa cagaaagaag
      601 gaatta
//
LOCUS       DAJTMK010000432          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTMK010000432 DAJTMK010000000
VERSION     DAJTMK010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.441"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..286
                     /locus_tag="OO135_004431"
     CDS             <1..286
                     /locus_tag="OO135_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399965.1"
                     /translation="DWFSLWGPWHAVLKRTEADRWALAEEQKYEMLENEYPQRVADRL
                     KASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHS"
     gene            complement(287..466)
                     /locus_tag="OO135_004432"
     CDS             complement(287..466)
                     /locus_tag="OO135_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399966.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(466..>605)
                     /locus_tag="OO135_004433"
     CDS             complement(466..>605)
                     /locus_tag="OO135_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399967.1"
                     /translation="LEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDV
                     E"
BASE COUNT          139 a          179 c          164 g          123 t
ORIGIN      
        1 ggactggttc tccctctggg gaccatggca tgctgtactg aagcgtacgg aagctgaccg
       61 ctgggcgctg gcagaagagc agaaatatga gatgctggag aatgagtacc ctcagagggt
      121 ggctgaccgg ctgaaagcat caggtctgag cggtgatgcg gatgcggaga gggaagccgg
      181 tgcacaggtg atgcgtgaga ctgaacagca gatttaccgt cagctgactg acgaggtact
      241 ggccctgcga ttgcctgaaa acggctcaca actgcaccat tcataatcac atcgcataaa
      301 ccacagaccg gactgactcc ggaaaaacag aggcccgccc ccgggcctcc ccggattcat
      361 ccgtttccct gttcagcctg acagcacgcc cccgcggccg gatgacagac tccgcttcgg
      421 taagcaaagc ggtcttctgt gattccgcca gttgcggctt attcattact caacgtcaaa
      481 cgcccgaatt gaagccaaat catccagacc gctcagctcc tctttcattt cacgctgacg
      541 gcgataaatc tcatcgttgc gatcgacctg cgcctgcacc attgctgccg ccagttcttc
      601 cagtt
//
LOCUS       DAJTMK010000433          594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTMK010000433 DAJTMK010000000
VERSION     DAJTMK010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.442"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..65)
                     /locus_tag="OO135_004434"
     CDS             complement(<1..65)
                     /locus_tag="OO135_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein E"
                     /protein_id="HCR5399968.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRAL"
     gene            complement(123..470)
                     /locus_tag="OO135_004435"
     CDS             complement(123..470)
                     /locus_tag="OO135_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5399969.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     GATGKLVVWDGQKAGSAVGILVLPLEGTEAVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
BASE COUNT          123 a          176 c          149 g          146 t
ORIGIN      
        1 agcgcacgga atttaacttt ttgttcggta taaccgagta actggcgggt cgtaaacaat
       61 cccataaatc agttcctttc attaagaaat cagtcaggcc aacgcggtgg cctgataacg
      121 tgttacggca gcgccgcgtg actcagggcg gtgccggcaa aggcatttgc ctttttgtgt
      181 tcatccacac tgtcaggcca gcggattgcc tccgtcgcaa aggtccccga cttgtaatac
      241 gtcagcaccg cctctgtgcc ttcaagcggc agtaccagta tgccaaccgc actaccggct
      301 ttctgtccat cccagaccac cagtttcccg gtggccccat ccagcatcag gggtgtcagt
      361 gccggtgttg ccgaggaaat cccgctgctg cctgtggcgg tgtgagccgg atcattaccg
      421 gcaaaaatac gtacttccgc acgctgttca gtgatggttt tcgtcaccat attgttaaaa
      481 cctcgtattg atgttcagca ctgacttcat ggcatggcca tgagcatttt cacgtccgca
      541 tcaccgtctt ctgacgcctg tgacacgctc cccctggacc gcagccggtg aatg
//
LOCUS       DAJTMK010000434          593 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTMK010000434 DAJTMK010000000
VERSION     DAJTMK010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.443"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..65)
                     /locus_tag="OO135_004436"
     CDS             complement(<1..65)
                     /locus_tag="OO135_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein E"
                     /protein_id="HCR5399970.1"
                     /translation="MGLFTTRQLLSYTEQKVKFRAL"
     gene            complement(123..470)
                     /locus_tag="OO135_004437"
     CDS             complement(123..470)
                     /locus_tag="OO135_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5399971.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
     gene            complement(507..>593)
                     /locus_tag="OO135_004438"
     CDS             complement(507..>593)
                     /locus_tag="OO135_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399972.1"
                     /translation="HSPAAVRGGVSQTSADGDADVKMLMAMP"
BASE COUNT          128 a          171 c          146 g          148 t
ORIGIN      
        1 agcgcacgga atttaacttt ttgttcggta taactgagta actggcgggt cgtaaacaat
       61 cccataaatc agttcctttc attcagaaat cagtcaggcc accatggtgg cctgataacg
      121 tgttacggca gagccgcgtg actcagggcg gtgccggcaa aggcatttgc ctttttgtgt
      181 tcatccacac tgtcaggcca gcggattgcc tccgtcgcaa aggtccccga cttgtaatag
      241 gtcagcaccg tctctgtgcc ttcaagcggc agtaccagta tgccaaccgc actaccggct
      301 ttctgtccat cccagaccac cagtttcccg gtggcttcat ccagcatcag gggcgtcaga
      361 gccggtgttg cagaagaaat cccgctgctg cctgtggcgg tatgagccgg atcattacca
      421 gcaaaaatac gtacttccgc acgctgttca gtgatggttt tcgtcaccat tttgttaaaa
      481 cctcatattg atggtcagca ctgacttcat ggcatggcca tgagcatttt cacgtccgca
      541 tcaccgtctg ctgacgtctg tgacacgcca ccccgcaccg ctgccggtga atg
//
LOCUS       DAJTMK010000435          561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTMK010000435 DAJTMK010000000
VERSION     DAJTMK010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.444"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..>561)
                     /locus_tag="OO135_004439"
     CDS             complement(<1..>561)
                     /locus_tag="OO135_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCR5399973.1"
                     /translation="TYDGNVGEQYNDAWFGDSANENIMQFSDIYLTTRGFLPFAPEAD
                     FWVGKHKLPQYEIQMLDWKTLTTDVAAGVGIENWALGVGLFDMSLSRDDVDVYSRDFS
                     RTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSAPNKTDQQQDNENDNSYFEMKDSWML
                     TSVLRQNLQRDTFNEFTLQVANNSYAS"
BASE COUNT          154 a          132 c          115 g          160 t
ORIGIN      
        1 gctggcatag gaattattgg caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag
       61 gttttgccgt aaaacagaag tcagcatcca gctatctttc atttcaaaat aactgttgtc
      121 attttcgtta tcttgttgtt gatccgtttt attaggtgcg gaatatttag ccattaatga
      181 taatgtcgca tcatcccata acgggatatt gcgataacga acatccacag aattggtatt
      241 catctggctg gtacgcgaaa aatcacgaga gtaaacatcg acatcatcac ggcttaagga
      301 catatcaaac agccctacac caagtgccca gttttcaatc cccacacccg cggcgacatc
      361 cgtggttaag gttttccagt ccagcatctg gatctcatat tgcgggagtt tatgtttgcc
      421 gacccagaag tctgcctctg gtgcgaaggg taaaaaacct cgcgttgtca ggtaaatatc
      481 gctgaactgc atgatatttt cattggcact gtcaccaaac caggcatcgt tatactgctc
      541 acccacgttg ccatcatagg t
//
LOCUS       DAJTMK010000436          553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTMK010000436 DAJTMK010000000
VERSION     DAJTMK010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.445"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..288)
                     /locus_tag="OO135_004440"
     CDS             complement(<1..288)
                     /locus_tag="OO135_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCR5399974.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVC"
     gene            complement(293..>553)
                     /locus_tag="OO135_004441"
     CDS             complement(293..>553)
                     /locus_tag="OO135_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5399975.1"
                     /translation="SASFVLSTPTETDGAVFPGRIMLANTCTWTYRGDECGYHGPAVA
                     DEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          124 a          179 c          140 g          110 t
ORIGIN      
        1 gcagaccagc caccacggca aatcactctg cacctgcagc cgccggtcag cctcactcag
       61 ccagggcaga ccaccgggat gactgtggac cagtgccaca atctcaccct gcatctctgc
      121 ctgcagccag tcctccggag ccatccggaa ataatcctcc ggctcaccgg aaatattcac
      181 gcagggaaaa tatctttccc cctccggcgc tctcaccacg aagccgcacg actccgctgg
      241 cgcacatcgc cgggcgtgcg ccagaatcgc tgattctgtc tctgtcatgg gattactgcg
      301 aaagtttgtt aatggaaagg aagccgccaa agttgccgac gttattgcga aacttacagc
      361 cgctcaggca tttgctgcat ttatccttcg tgatatcgga cgtcggctgg tcatattcat
      421 ccgcgactgc cggaccgtga taaccgcact catcaccgcg ataggtccag gtgcaggtgt
      481 tggccagcat gatacgtccc ggaaaaacag cgccatccgt ttccgtcggc gtggacagta
      541 caaaggaggc act
//
LOCUS       DAJTMK010000437          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTMK010000437 DAJTMK010000000
VERSION     DAJTMK010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.446"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            177..365
                     /locus_tag="OO135_004442"
     CDS             177..365
                     /locus_tag="OO135_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399976.1"
                     /translation="MLTRKSIVPVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            375..>550
                     /locus_tag="OO135_004443"
     CDS             375..>550
                     /locus_tag="OO135_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5399977.1"
                     /translation="MLSAFTPRSLKRLFTANQCWTSFLDAGGLRDIGVEAVTKMLACG
                     TRILGVKEYNRDKP"
BASE COUNT          120 a          158 c          151 g          121 t
ORIGIN      
        1 ggagggctca ccaccgctga acgtcagcgt ctgaaagagc tggagcgtga aaatcgtgaa
       61 ccacggcagc ctgacggctg caaattgaaa accgcccctg ttcgtcctgt tgttgccgca
      121 cgctccgcag aatatgtatg ctctgaggac ctgttttatt aaggactcat cctcttatgt
      181 tgacccgcaa aagtatagtt cccgttctgc tctctgttgg tgctgagaag ctctcacaac
      241 gtgaatggga ctggatgaag atgctcaaac ccatggaccc accacctgcg atggtcgcgg
      301 cctccatcct ggagcgccgt ggcgacacag cagcccttac ccggctacag gataccgggg
      361 gttaatccgt cctcatgttg tccgctttta ctccccgatc gctgaaacgc ctgttcacag
      421 ccaaccagtg ctggacgtcc ttcctggatg cgggcggtct gcgcgatatc ggggttgagg
      481 ctgtcacaaa aatgctggcc tgcggcacgc ggatactggg agtgaaggaa tacaaccgcg
      541 ataaacctga
//
LOCUS       DAJTMK010000438          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTMK010000438 DAJTMK010000000
VERSION     DAJTMK010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.450"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..243
                     /locus_tag="OO135_004444"
     CDS             <1..243
                     /locus_tag="OO135_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5399978.1"
                     /translation="HDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVGCFVPTLIL
                     VMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
BASE COUNT          146 a           94 c          130 g          158 t
ORIGIN      
        1 catgatcgcg ggcgttccgg cgcatgggca tttctgatga ttgtggcgtg gatgctgctg
       61 gcgggtaact gggcgatttt accgggtgtc tggcaatggg cggtggggtg ttttgtcccg
      121 acgttgattc tggtgatgat gcttatcgat cttggtgcgt ttgttggcac acaaggcgaa
      181 aataaatatg gtaaagatac tcaggatgtg aagtataaag ccgacaataa atcaagtaat
      241 taacctttaa tatatctctt actgctattt ttttccggga ttgttatttc cacgatgcaa
      301 atgacaatac ccggaaaatc cttctgaact cttcacctta agcaatatca aaaaaaatgg
      361 taatccataa gatcattact tgtttgttct tcccttaacg gcgcattatt cttaagtcgt
      421 aatcgacaga gaggcgaata tacagaggtg ccctatgaaa gatgtcgtag ataaatgcag
      481 tactaaagga tgtgcgattg atatcggtac ggtgattgat aacgacaa
//
LOCUS       DAJTMK010000439          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTMK010000439 DAJTMK010000000
VERSION     DAJTMK010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.451"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
BASE COUNT          176 a          118 c           97 g          136 t
ORIGIN      
        1 gttcagggcc gacaaacacg ttgtccctga gttcacattc ataaatgttg accggtttga
       61 caatacggac attggtccca taaatcacat cccggacagc acattccaga aatttcatac
      121 tcacacgtcc tttaaataca gatgacattt gccggaatta taccgcccag attcacttgc
      181 catgtttcag aatagcttat aagccgcact tttaccgata ctcagaaaca aatcaggtct
      241 gtatccactt cagccggaaa ccggatctgc gcggcgggga acgggcaaga aatatgataa
      301 ttaagcaaca cacataaaat catattgcat aatcgcatct gaatcgaaca cttattcgca
      361 tcgctatcga tcatcatttc gcatctcata cccttaccca caagttgaaa taatagtatt
      421 atattgaaaa gacagagatt atttcagagg tgttatggaa aactgggtta ttcaggagct
      481 aaaatcactg gatatagggg actcaagact tgataaaaga gtaaaac
//
LOCUS       DAJTMK010000440          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTMK010000440 DAJTMK010000000
VERSION     DAJTMK010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.452"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            33..>526
                     /locus_tag="OO135_004445"
     CDS             33..>526
                     /locus_tag="OO135_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5399979.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKMAN
                     HLSE"
BASE COUNT          135 a          127 c          156 g          108 t
ORIGIN      
        1 tcttcctgat ttaatttatc gcgagaaaaa ctatgtctgt agtgatatca ggtgcgctga
       61 ctgatggcgc aggcatcccc atgtccggat gccagattat tctgaaatcc cgtgtaaaca
      121 cctcagaagt ggtgatgcgt accagggctg atgtagtgac cggaaataac ggtgaatatt
      181 cgtttgaggc acaggtcgga aaatattgcg tgtatctgaa acgggactgg cgcgacgagt
      241 actgtgttgg cgacattgct gtatacgacg actcaaagcc cggcacgctg aatgactttc
      301 tgactgcccc cgatgaaggc gacctgaagc cggatgtcgt caaacgcttt gaggaaatgg
      361 tggcgcaggc gcagcagagc gccggggccg cagccggaaa cgcacagcag acggcgcagg
      421 atgtggcggc agccgcaggt tatgcccgcg cagcagaaca ggccaaaaat gacattgatg
      481 ctgcgctgac cggcactctg aaaatggcta accatctgtc agaaat
//
LOCUS       DAJTMK010000441          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTMK010000441 DAJTMK010000000
VERSION     DAJTMK010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.453"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..230)
                     /locus_tag="OO135_004446"
     CDS             complement(<1..230)
                     /locus_tag="OO135_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5399980.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFT"
     gene            complement(211..>525)
                     /locus_tag="OO135_004447"
     CDS             complement(211..>525)
                     /locus_tag="OO135_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5399981.1"
                     /translation="QLDMHFSGLTYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPD
                     EDKMLMQKAAGLAGGVRFGGDGGRDILSSADVADVMVDDAALMMASAGIPGGVRYVPA
                     GW"
BASE COUNT          125 a          173 c          122 g          105 t
ORIGIN      
        1 gtaaactgtg cgggcagggt tcgcatggcc gctttatact gcccgacgga aatcccggct
       61 ttttgtgcag ccagcgcctg gcggctcagg ccctgttcaa cagcactggc ggtttttctg
      121 gcgtcggtat ccagacctga aaaatgacgc cttacccggc tcatctgctc atcgaaacgg
      181 acagcatcca gactcaggtc aataacaaga tcaccaaccg gctgggacat atctcacacc
      241 tcccggaatc cccgctgaag ccatcattaa tgcggcatca tccaccatga catccgccac
      301 atccgcagac gataaaatat cgcgccctcc gtccccaccg aaccggacgc ctccggcaag
      361 tcctgccgct ttctgcatca gcattttgtc ctcatccggc ctctccacct gctcttcctc
      421 atgccggggg acaagcagac tgaaatcaga gggatgcata tccggatcgc aaaaaaacag
      481 gctgagtaca gcgtacgtca gcccggaaaa atgcatatcc agctg
//
LOCUS       DAJTMK010000442          521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTMK010000442 DAJTMK010000000
VERSION     DAJTMK010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.454"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(84..170)
                     /locus_tag="OO135_004448"
                     /pseudo
     CDS             complement(84..170)
                     /locus_tag="OO135_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          145 a          130 c          123 g          123 t
ORIGIN      
        1 ctgggttatc gctcgccacg ggaatatcta cggcagcggg ccagtaatgg gttaagtgat
       61 aacaggtgtc tggaaatata ggggcaaatc caatggcgca gatacgcttg cggatcgata
      121 ccattcagac agcaggtccg ttcactaccg taatcgctgc cgaagaacac attgattgcc
      181 cctcaataat cctgccgtca acgtcaaaat tgctggtcgg gctcgactta tttaattgtg
      241 taaacagggc ctctcagcga aaggccctta tgcatcacct gacgtcgtta aaagtcacta
      301 cacccaagat gacgttcaat ggcaccatgc gattcaatgt aagcctgggc cgtctgttcc
      361 agtacaccag gctcagcgtt gtatgtgtta gctgcatcaa ataccaacga cagcacttca
      421 ggatggattt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
      481 cccgacaacg cagaccgtac catggcaaag caaaagttca a
//
LOCUS       DAJTMK010000443          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTMK010000443 DAJTMK010000000
VERSION     DAJTMK010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.455"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            233..513
                     /locus_tag="OO135_004449"
                     /pseudo
     CDS             233..513
                     /locus_tag="OO135_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          131 a          135 c          127 g          120 t
ORIGIN      
        1 cccgccagcc cggctaagat ggaggcacgg cacagcccgc acacgacgct gaggctacag
       61 agctgaaaac ccacaaggcc atgccccgca tggccgggta aagcggagcc ggcggcgact
      121 tcctttgtgc tctgtccggg accggcagga aaaaggcggg gataatgaga tggtcacccc
      181 caccctaaca gcagtacgct gttaaggtga atcccttaac ggagagacca tcatacagaa
      241 catcacactt attggtatcg atctcggaaa gcattccttc cacattcatt gtcaagacaa
      301 gtctggtaag gctcttctgc gtaaaaaatt tacccagtac taaactgatg cagtttctgg
      361 caacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttttggctc
      421 gccgtatctc tgattttgga catgaaacta agctgatttc tccacagttc gttcgcccgt
      481 ttgtcaaaag taataagaat gattttgttg atg
//
LOCUS       DAJTMK010000444          513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTMK010000444 DAJTMK010000000
VERSION     DAJTMK010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.456"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            117..>513
                     /locus_tag="OO135_004450"
     CDS             117..>513
                     /locus_tag="OO135_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399982.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFS"
BASE COUNT          204 a           88 c           73 g          148 t
ORIGIN      
        1 ggctattgtc atacactcaa attaaagata ggttctaaat aaatgagcgt tttttgatag
       61 tctatttcat taggtaatat atatttgtaa caaatcaatc aaaatggaat aaaatcatgc
      121 taccatctat ttcaatcaac aataccagcg caacttaccc agaatccatt aatgaaaata
      181 acaatgatga agttaatgga ttagtacaag agctcaaaaa cctttttaat ggtaaggaag
      241 gaataagcac ctgtattaaa catctacttg agcttataaa aaacgccata cgagtaaacg
      301 acgatcctta tagatttaat attaataatt cctcagttac ttatattgat attggctcca
      361 atgatacaga ccatattact attggtatcg acaaccaaga accaatagaa ttacctgcga
      421 actataaaga caaagaactc gtccgtacta tcattaatga caacatagtt gagaagactc
      481 atgatatcaa taacaaggaa atgatcttca gcg
//
LOCUS       DAJTMK010000445          500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTMK010000445 DAJTMK010000000
VERSION     DAJTMK010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.457"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..288
                     /locus_tag="OO135_004451"
     CDS             <1..288
                     /locus_tag="OO135_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HCR5399983.1"
                     /translation="ETIKKWRIHRSTAESLLDFARHYNAIVRGWIEYYGKFWSRNFSY
                     RLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKLYPKLFAHWYLLRASNE"
     gene            362..481
                     /locus_tag="OO135_004452"
                     /pseudo
     CDS             362..481
                     /locus_tag="OO135_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          131 a          108 c          148 g          113 t
ORIGIN      
        1 gaaacaatca agaagtggcg tatacatcgc tcaacagctg agagtttgct ggattttgcg
       61 aggcactaca atgcgatagt gagaggctgg attgagtact atggaaagtt ctggtccaga
      121 aacttcagct atcgactgtg gagtgcaatg cagtcacgtt tgcttaagtg gatgcagtct
      181 aaatacagac tttcgaaccg gaaggctcag cgaaagctgg cgctggtaag gaaactgtat
      241 ccgaagctat ttgcccactg gtatttacta cgtgcatcga atgagtgatc aagagccgta
      301 tgacgcgaga gtgtcacgta cggttccgtg agcagccgga gagtgaaact ctctccgacg
      361 gggttccgat agcggtggcg aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg
      421 tctgaacaat gtggagccag aaaaatggct gcgttacgtc attgagcata tccaggactg
      481 accggcaaac cgggtacgcg
//
LOCUS       DAJTMK010000446          497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTMK010000446 DAJTMK010000000
VERSION     DAJTMK010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.458"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            60..332
                     /locus_tag="OO135_004453"
     CDS             60..332
                     /locus_tag="OO135_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399984.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            313..>497
                     /locus_tag="OO135_004454"
     CDS             313..>497
                     /locus_tag="OO135_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5399985.1"
                     /translation="MTRSGGDFQPRSLKRLFTANQCWTSFLDAGGLRDIGVEAVTKML
                     ACGTRILGVKEYICDKP"
BASE COUNT          129 a          127 c          129 g          112 t
ORIGIN      
        1 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagttccca
       61 tgtcacacaa cctggagcac cagaaagtcc acaccagaat ggtgaaagag gttctgaaag
      121 ccgtcgccag agccaataat cacccttata agtccgtatt tgcagacttt atcaccggtc
      181 atccctcctg cacggtctgt ttctgggaaa ccttccacaa aatgtacccg gattcaccgt
      241 acgaatacgt cacgttctgc catacctgcc gtcgttttga tttatacgaa acagaagcgg
      301 agatgaaggc ggatgacccg aagtggtggt gattttcagc cccgatcgct gaaacgcctg
      361 ttcacagcca accagtgctg gacgtccttc ctggatgcgg gcggtctgcg tgatatcggg
      421 gttgaggctg tcacaaaaat gctggcctgc ggcacgcgga tactgggagt gaaggaatac
      481 atctgcgata aacctga
//
LOCUS       DAJTMK010000447          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTMK010000447 DAJTMK010000000
VERSION     DAJTMK010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.459"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(6..81)
                     /locus_tag="OO135_004455"
     tRNA            complement(6..81)
                     /locus_tag="OO135_004455"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46..48),aa:Lys,seq:ttt)
     gene            complement(84..159)
                     /locus_tag="OO135_004456"
     tRNA            complement(84..159)
                     /locus_tag="OO135_004456"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Val,seq:tac)
     gene            complement(195..270)
                     /locus_tag="OO135_004457"
     tRNA            complement(195..270)
                     /locus_tag="OO135_004457"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Lys,seq:ttt)
     gene            complement(274..349)
                     /locus_tag="OO135_004458"
     tRNA            complement(274..349)
                     /locus_tag="OO135_004458"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Val,seq:tac)
BASE COUNT          116 a          138 c          127 g          112 t
ORIGIN      
        1 aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
       61 taccgactga gctaacgacc cggtggtggg tgatgacggg atcgaaccgc cgaccccctc
      121 cttgtaaggg aggtgctctc ccagctgagc taatcaccca atactacgct ggatactaca
      181 actaactatg ttagtggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
      241 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
      301 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
      361 ggatactata aactacttcc accgaaccca tcgtaattag tggtgggtcg ggcaggatga
      421 ctcgcttcgc tcgccctacg ggccgttgct gacgcaacgt tatcctacac gcttgacatc
      481 tgtcttcgat gct
//
LOCUS       DAJTMK010000448          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTMK010000448 DAJTMK010000000
VERSION     DAJTMK010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.460"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>488
                     /locus_tag="OO135_004459"
     CDS             <1..>488
                     /locus_tag="OO135_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCR5399986.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTESQWRSLV
                     LKA"
BASE COUNT          105 a          127 c          154 g          102 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagtggag aagcctggtg
      481 ctgaaagc
//
LOCUS       DAJTMK010000449          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTMK010000449 DAJTMK010000000
VERSION     DAJTMK010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.461"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>482
                     /locus_tag="OO135_004460"
     CDS             <1..>482
                     /locus_tag="OO135_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399987.1"
                     /translation="QLSRPSEINRLKALVAKLQRMQFGKSSEKLRAKTERRIQEAQER
                     ISALQEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGEL
                     SPLGCDVSEQLELISSAFKVIEKQRPKLACRRCDHIVQAPVPSKPIARSYAGAGLLAH
                     V"
BASE COUNT          116 a          140 c          135 g           91 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gt
//
LOCUS       DAJTMK010000450          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJTMK010000450 DAJTMK010000000
VERSION     DAJTMK010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.463"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(9..>467)
                     /locus_tag="OO135_004461"
     CDS             complement(9..>467)
                     /locus_tag="OO135_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399988.1"
                     /translation="TARYLGRYLKKPPIAASRLAHYNGGASLNFRYLDHKTGETATET
                     LTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYA
                     QMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT           87 a          158 c          113 g          109 t
ORIGIN      
        1 agcctgtgtc aggccggcat atacctcagc agactgatat cccgcgcgtt tttcttcagc
       61 ccctccacat tcagtcccgc gatggcccgg cggtatacca tccggccgcc acacagcacg
      121 cattcgaacg ggtcacgact caggaactgt ttcaccattt gtgcatagca cactttcgcc
      181 actggttccg gtttatccat ccccagagca cggtacacct gcggcagctt ctctccacac
      241 acccggttgg caaggaaccc gaagtatctc accatcttaa aaaacttctc cgggatgtgc
      301 tgtttcagcc tcgcgaccag ctcacgctgt gtcagcgttt ccgtcgccgt ttctcccgtt
      361 ttgtggtcca ggtaacggaa gttcaggctc gcccctccgt tgtaatgtgc cagccgggag
      421 gccgctatcg gcggcttctt cagataacga cccaggtagc gcgccgt
//
LOCUS       DAJTMK010000451          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJTMK010000451 DAJTMK010000000
VERSION     DAJTMK010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.464"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>463
                     /gene="ipaH9.8"
                     /locus_tag="OO135_004462"
     CDS             <1..>463
                     /gene="ipaH9.8"
                     /locus_tag="OO135_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5399989.1"
                     /translation="LDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADA
                     TESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDIARDKVR
                     TLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVR"
BASE COUNT          104 a          132 c          120 g          107 t
ORIGIN      
        1 cttgaccgcc tttccgatac cgtctctgca cgcaatacct ccggattccg tgaacaggtc
       61 gctgcatggc tggaaaaact cagtgcctct gcggagcttc gacagcagtc tttcgctgtt
      121 gctgctgatg ccactgagag ctgtgaggac cgtgtcgcgc tcacatggaa caatctccgg
      181 aaaaccctcc tggtccatca ggcatcagaa ggccttttcg ataatgatac cggcgctctg
      241 ctctccctgg gcagggaaat gttccgcctc gaaattctgg aggacattgc ccgggataaa
      301 gtcagaactc tccattttgt ggatgagata gaagtctacc tggccttcca gaccatgctc
      361 gcagagaaac ttcagctctc cactgccgtg aaggaaatgc gtttctatgg cgtgtcggga
      421 gtgacagcaa atgacctccg cactgccgaa gccatggtca gaa
//
LOCUS       DAJTMK010000452          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJTMK010000452 DAJTMK010000000
VERSION     DAJTMK010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.465"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>452
                     /locus_tag="OO135_004463"
     CDS             <1..>452
                     /locus_tag="OO135_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5399990.1"
                     /translation="NDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEY
                     PQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAA
                     GKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEA"
BASE COUNT          119 a          113 c          121 g           99 t
ORIGIN      
        1 aatgacgaat tatatcttga tggcaacgct cgtcagaacc tagccacttt ctgccagacc
       61 tgggacgacg aaaatgtcca caaattgatg gatttatcca ttaacaaaaa ctggatcgac
      121 aaagaagaat atccgcaatc cgcagccatc gacctgcgtt gcgtaaatat ggttgccgat
      181 ctgtggcatg cgcctgcgcc gaaaaatggt caggccgttg gcaccaacac cattggttct
      241 tccgaggcct gtatgctcgg cgggatggcg atgaaatggc gttggcgcaa gcgtatggaa
      301 gctgcaggca aaccaacgga taaaccaaac ctggtgtgcg gtccggtaca aatctgctgg
      361 cataaattcg cccgctactg ggatgtggag ctgcgtgaga tccctatgcg ccccggtcag
      421 ttgtttatgg acccgaaacg catgattgaa gc
//
LOCUS       DAJTMK010000453          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJTMK010000453 DAJTMK010000000
VERSION     DAJTMK010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.466"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..451
                     /locus_tag="OO135_004464"
                     /pseudo
     CDS             1..451
                     /locus_tag="OO135_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          137 a          108 c          117 g           89 t
ORIGIN      
        1 cgccttgagc gagatatatt aaaaaaagca acagcgtatt ttgcacagga gtcgctgaaa
       61 aatacgcgtt aatcgaacaa tggcgacaac aatttcccat tgaagcgatg tgtcaggtat
      121 ttggtgtatc caggagcggt tattacaact gggtacagca tgaaccctca gacagaaaac
      181 aaagtgatga gcggctaaaa ctggagatta aggtggcaca tatccgcact cgcgaaacat
      241 atggaacccg gcggctccag acggagctgg cagagaatgg catcatcgtt ggtcgtgacc
      301 gactggcacg tcttcgtaag gagctaaggc tacgctgtaa gcagaaacgc aagttcagag
      361 cgactacgaa cccgaaccac aatctgccag ttgcgccaaa tctgctgaac cagacgttcg
      421 ctcctacagc accaaatcag gtctgggtgg c
//
LOCUS       DAJTMK010000454          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJTMK010000454 DAJTMK010000000
VERSION     DAJTMK010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.468"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..>430)
                     /locus_tag="OO135_004465"
     CDS             complement(<1..>430)
                     /locus_tag="OO135_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5399991.1"
                     /translation="LKGGWIFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINI
                     PGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTSKGQNSF
                     ASQLAKELHTNVIGPDTLWTWWGRGTNGKLKMDTVLTAPT"
BASE COUNT          120 a          100 c           72 g          138 t
ORIGIN      
        1 ttcgttggtg ctgttagcac tgtatccatt tttaacttac cattagttcc tcgcccccac
       61 cacgtccata gcgtgtcagg tcctattaca tttgtatgta actctttagc taattggcta
      121 gcaaatgaat tttgtccttt acttgtatta caagaaaata accaaactgt catcccatct
      181 ttataattcc catcaaattt aattagatat gctagatctt tagctgtcat gatactgcgc
      241 gttgcggatt caatagatgt gggggtacca tgccccccga ttgtgaaaac gcctgggata
      301 ttaatctcat cagctacaga atggataaga tcactttcgg ggtataaatt tatatcaacc
      361 aaaccttgcg gatctacatt tatgaccgga ttcaacggat actgataaaa aatccatccc
      421 cccttcagcc
//
LOCUS       DAJTMK010000455          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJTMK010000455 DAJTMK010000000
VERSION     DAJTMK010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.469"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..371
                     /locus_tag="OO135_004466"
     CDS             <1..371
                     /locus_tag="OO135_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCR5399992.1"
                     /translation="ALVYSHGEDVYSYESGAHSDFDSIRTVIRPAWIWNTWNQTGLEL
                     GWFKQQNKTQQGVTLNESAYKTTLWHALKVGESILGSRPEIRFYGTYINILDNELSNF
                     KFNENSKDEFMAGIQAEVWW"
BASE COUNT          130 a           65 c          103 g          120 t
ORIGIN      
        1 acgcgcttgt ctattctcac ggcgaagatg tttatagtta tgaaagtggt gctcatagtg
       61 attttgacag tattcgcact gtaataagac cggcctggat ctggaataca tggaatcaga
      121 cggggcttga attaggctgg tttaagcaac agaacaaaac tcagcagggt gtaacactaa
      181 atgaatcggc ttataaaacg acactctggc atgcattgaa agtgggtgaa agcattttag
      241 gttcacgacc agaaattcgc ttctatggaa cgtatatcaa tattctggat aacgaattat
      301 cgaattttaa gtttaatgag aacagcaaag acgagtttat ggccggcatc caggcggaag
      361 tctggtggta atctatttta gttaaggagt tgtgtatgtc aggatttaaa aaaggttt
//
LOCUS       DAJTMK010000456          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJTMK010000456 DAJTMK010000000
VERSION     DAJTMK010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.470"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            111..>418
                     /locus_tag="OO135_004467"
     CDS             111..>418
                     /locus_tag="OO135_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5399993.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     R"
BASE COUNT          101 a          117 c          109 g           91 t
ORIGIN      
        1 tcgggttatc acagagtgcc tgagacagtg tcctgccgga ggtcacttca gggactctgc
       61 gtatttttta cgacgtggct attccgtatg aaccatacgg agatttaacc atgacctaca
      121 aatacaaccc cttctggcag caacgtattc gtgagacggt gcggcacgca ctgaatgttc
      181 atccccgcct gacggcattg cgggttgacc tgcgtctccc ggatgtaccg gcagcaacgg
      241 acgcagctgt gatatcccgc ttcatcaatg ccctgaaagc ccgaatcgac gcttaccaga
      301 aacgtaagca tcgggaaggt aaacgcgtgc atcccacaac cctgcattac gtctgggccc
      361 gggagtttgg ggagtgcaaa ggtaaaaaac actatcacct gatgctgctg gtcaaccg
//
LOCUS       DAJTMK010000457          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJTMK010000457 DAJTMK010000000
VERSION     DAJTMK010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.474"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            1..396
                     /locus_tag="OO135_004468"
                     /pseudo
     CDS             1..396
                     /locus_tag="OO135_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           94 a          108 c          109 g           85 t
ORIGIN      
        1 acggcaaaag cactgccgga catcagcgct atctctgctc tcactgccgt aaaacatggc
       61 aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatt attgatatgg
      121 ccatgaatgg cgttggatgc cgggcaactg cacgcattat gggcgttagc ctcaacacga
      181 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag
      241 tgacgtcatc gtctgcgcgg aaatggacga acagtgggga tacgtcgggg ctaaatcgcg
      301 ccagcgctgg ctgttttacg cgtatgacag gctccggaag acggttgttg cgcacgtatt
      361 cggtgaacgc actatggcga cgctggggcg tcttat
//
LOCUS       DAJTMK010000458          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJTMK010000458 DAJTMK010000000
VERSION     DAJTMK010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.475"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..341
                     /locus_tag="OO135_004469"
     CDS             <1..341
                     /locus_tag="OO135_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5399994.1"
                     /translation="QPGAVVARIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVP
                     VALTDTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRA
                     LIREMKGGTR"
BASE COUNT          104 a          103 c          105 g           78 t
ORIGIN      
        1 tacagcctgg ggctgttgtt gcccggatcg ccagagaaca cgatatcaat aataacctgc
       61 tgtttaaatg gaaaagccag tacgaggacg gcttactgag cgatgatgac atacaggaat
      121 gcatgcctgt cccggtggca ctgactgata cgccggagcc gaccagacca gttacaaatc
      181 ccttctggcg taacaagcat gatgagcgcc ctgagggggc tcccggaaac gtcccacggt
      241 gcgagctgca tcttaaatca ggtgtggtaa aactgtttga ccctctcact ccggaactgt
      301 tacgggcgct aatccgcgaa atgaaaggag gtacccgatg ataacgctgc caaccggtac
      361 cagaatctgg atcatcgctg gcatcacaga
//
LOCUS       DAJTMK010000459          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJTMK010000459 DAJTMK010000000
VERSION     DAJTMK010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.476"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..123
                     /locus_tag="OO135_004470"
     CDS             <1..123
                     /locus_tag="OO135_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5399995.1"
                     /translation="QAKGKNVNITIVAVARELAGFIWDMGRIAMSVAQQPQCHK"
BASE COUNT          122 a           86 c           95 g           84 t
ORIGIN      
        1 caggccaaag gaaagaatgt caatattaca attgttgctg ttgcacgtga gctggcgggt
       61 tttatctggg atatgggccg aatagcaatg tctgtcgcac aacaaccaca atgtcacaaa
      121 taaaaaatga gaataaccag ttccctgaag gcgacgtagc ccggtacaag agtaacccac
      181 gttagcgctt ggcgacggcg gaacaaagct gattcgcgac gtaagaaagt ggcaggctca
      241 tatgacggac ctctgtaatg cggtatccaa cccgcgaata tcagcgtgat tcaccgtcgg
      301 atttactgct acgtcgccct cagggaatta aacctaaaaa gaaaatcgtc aaaattagag
      361 attccggttt gacacggaaa gtcatat
//
LOCUS       DAJTMK010000460          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJTMK010000460 DAJTMK010000000
VERSION     DAJTMK010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.478"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(<1..312)
                     /locus_tag="OO135_004471"
     CDS             complement(<1..312)
                     /locus_tag="OO135_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5399996.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EF"
BASE COUNT           80 a          136 c           80 g           75 t
ORIGIN      
        1 aaactccgcg ctgaactcaa cgcgcaacat cccgacccgc gcagaccacc cggcacaggt
       61 cacctttatc tgccggtatg catagggtgg cttccacaaa aatgccttcc agccaccgtg
      121 ctctgccagg aacgcttcca gatgccgggc ctcctcccgg gtcacggaaa gcgtcaccct
      181 gtatgttttc aggtcagcat tcagccctgc cgccatacgc tgtgagtacc cgtcaccaaa
      241 acgcacttca cgcaccgatg gctgcgagtt cacctccata tccggcttca ctttccagcg
      301 aaaggttttc atccaccgct ccctgataac ataccgccat cacgcaactg cagccggagt
      361 tcatcctgtg c
//
LOCUS       DAJTMK010000461          362 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.480, whole genome shotgun sequence.
ACCESSION   DAJTMK010000461 DAJTMK010000000
VERSION     DAJTMK010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.480"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
BASE COUNT           97 a          107 c           84 g           74 t
ORIGIN      
        1 tgaaccgggc tataacctgc agtcggttgt tgaacgcctg acaacaggaa atcagccagg
       61 ccagcaacca cgcggactga cagagaatca gaacctgaat cagcctgaaa tcatacccgg
      121 ggcagtactt catcaccagt caccaatacc cgtgtcagta cttcgcggcc tgacgcgatc
      181 acccgactca gtacttctga gcccgcctcc ggtaccattc tctgtgggcc cgtacacaac
      241 aacagaaaag ggtgcccggc ccggggcgca accgtattac acttccggaa aatcgttata
      301 cgcagaaaaa tcggatcacg ctgtgcctgc tttcatgtaa tttacttacg gtgttataag
      361 ta
//
LOCUS       DAJTMK010000462          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJTMK010000462 DAJTMK010000000
VERSION     DAJTMK010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.482"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            186..>355
                     /locus_tag="OO135_004472"
     CDS             186..>355
                     /locus_tag="OO135_004472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399997.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDG"
BASE COUNT           94 a           67 c          108 g           86 t
ORIGIN      
        1 ttttatgcca ttaccggttt taaccaacag gattatcggt gggcatgaaa gaaaaccccg
       61 gtatctgctg ataccggggt ttctctttag catggcagaa atgtgtttca tgcttttcgg
      121 gcgaaggata tccgacttct gtacggaatg gcaagtggcg gttaatttat tcaggggaag
      181 gctgtatggg aaaaggtggc ggtaaggcac acactcctcg tgaggcgaag gataatctca
      241 aatccacgca gatgatgagt gtgattgatg cgattggtga gggaccgata gaaggtccgg
      301 tgaagggact gcagagtatt ctggtgaaca aaaccccact gacggacacg gacgg
//
LOCUS       DAJTMK010000463          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.483, whole genome shotgun sequence.
ACCESSION   DAJTMK010000463 DAJTMK010000000
VERSION     DAJTMK010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.483"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>352
                     /locus_tag="OO135_004473"
     CDS             <1..>352
                     /locus_tag="OO135_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5399998.1"
                     /translation="GGQATGKTWLAFTNVGNSNLGVATSGQGIRVVDAQNGATTEEGA
                     FALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTSMLTQAMDYDRILAGSRS
                     HQTGVNGENNSVRL"
BASE COUNT           87 a           97 c           99 g           69 t
ORIGIN      
        1 atggtggtca ggcaaccggc aaaacctggc ttgcgtttac aaatgtcgga aacagtaacc
       61 tcggggtggc aacctccgga cagggtatcc gggttgtgga tgcacagaat ggcgccacca
      121 cagaagaagg tgcgtttgcc ctgagtcgcc cgcttcaggc cggcgccttt aactacaccc
      181 tgaaccgtga cagcgatgaa gactggtacc tgcgcagtga aaatgcttat cgtgctgaag
      241 tccccctgta tacatccatg ctgacacagg caatggacta tgaccggatt ctggcaggct
      301 cccgcagcca tcagaccggt gtaaacggtg aaaataacag cgtccgtctc ag
//
LOCUS       DAJTMK010000464          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.487, whole genome shotgun sequence.
ACCESSION   DAJTMK010000464 DAJTMK010000000
VERSION     DAJTMK010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.487"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>319
                     /locus_tag="OO135_004474"
     CDS             <1..>319
                     /locus_tag="OO135_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5399999.1"
                     /translation="QYGGSIVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNPLLGAKV
                     LPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSD
                     NGGR"
BASE COUNT           56 a          109 c           96 g           58 t
ORIGIN      
        1 cagtatggcg gtagcattgt tcagggttca gccggggtgc gcattggtgc ccccaccggc
       61 gtggcctgtt cggtgtgccc cggcggagtg acgtccggcc atccggtcaa tcccctgctc
      121 ggtgcaaagg tccttcccgg tgaaaccgac atcgccctgc ccggcccgct gccgttcatc
      181 ctctcccgca cctacagcag ttaccggaca aaaacgcccg cgccggtggg gagcctcggc
      241 cccggctgga aaatgcctgc ggatatccgc ttacagctgc gcgataacac actgatactc
      301 agtgataacg gcggcagaa
//
LOCUS       DAJTMK010000465          299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.489, whole genome shotgun sequence.
ACCESSION   DAJTMK010000465 DAJTMK010000000
VERSION     DAJTMK010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.489"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            190..299
                     /locus_tag="OO135_004475"
                     /pseudo
     CDS             190..299
                     /locus_tag="OO135_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           86 a           67 c           77 g           69 t
ORIGIN      
        1 gattgttgcc agacaggaca attttcacaa gacgtcgctg atggggcgct taccttaccg
       61 caggacaggt ggtaactgaa aaatagtttg ctgaggcgac tggaaacaga agggcaagat
      121 cgattccgac agcagtgatg gtcagctacc tcatcgataa ctttgcaaca gtgcccttct
      181 tgaagcatca tgcttcatga agaaaaactg gcacgtcatc aacgtaaaca ggcgatgtat
      241 acccgaatgg tagccttccc ggcggtgaaa atgttcgaag agtatgactt cacgttcgt
//
LOCUS       DAJTMK010000466          296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.denovo.490, whole genome shotgun sequence.
ACCESSION   DAJTMK010000466 DAJTMK010000000
VERSION     DAJTMK010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.denovo.490"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..>296
                     /locus_tag="OO135_004476"
     CDS             <1..>296
                     /locus_tag="OO135_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5400000.1"
                     /translation="QGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKN
                     EWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT           86 a           68 c           84 g           58 t
ORIGIN      
        1 cagggcagtc attatacgag caggcagttc cggcagttat tgtggcgata ccagatcaga
       61 cagagtatga gccggcgcgg aaactgctgg gataacagcc caatggaacg cttcttcagg
      121 agtctgaaga acgaatggat gccggtggtg ggttacgtaa gcttcagcga ggcagctcac
      181 gccataacgg actatatcgt tggatattac agcgcactaa gaccgcacga atataacggt
      241 gggttacccc caaacgaatc ggaaaatcga tactggaaaa actctaactc ggtggc
//
LOCUS       DAJTMK010000467        32158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.027, whole genome shotgun sequence.
ACCESSION   DAJTMK010000467 DAJTMK010000000
VERSION     DAJTMK010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32158
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.027"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            561..800
                     /locus_tag="OO135_004477"
     CDS             561..800
                     /locus_tag="OO135_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5400001.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO135_004478"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="OO135_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /locus_tag="OO135_004479"
     CDS             2297..2770
                     /locus_tag="OO135_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5400002.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3390
                     /gene="sat2"
                     /locus_tag="OO135_004480"
                     /pseudo
     CDS             2865..3390
                     /gene="sat2"
                     /locus_tag="OO135_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
     gene            3459..3956
                     /locus_tag="OO135_004481"
     CDS             3459..3956
                     /locus_tag="OO135_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5400003.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4017..4388
                     /locus_tag="OO135_004482"
     CDS             4017..4388
                     /locus_tag="OO135_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5400004.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4798..5175
                     /locus_tag="OO135_004483"
     CDS             4798..5175
                     /locus_tag="OO135_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5400005.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5198..5350
                     /locus_tag="OO135_004484"
                     /pseudo
     CDS             5198..5350
                     /locus_tag="OO135_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5406..7022)
                     /locus_tag="OO135_004485"
     CDS             complement(5406..7022)
                     /locus_tag="OO135_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5400006.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7023..8549)
                     /locus_tag="OO135_004486"
     CDS             complement(7023..8549)
                     /locus_tag="OO135_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5400007.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8552..10219)
                     /locus_tag="OO135_004487"
     CDS             complement(8552..10219)
                     /locus_tag="OO135_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5400008.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10216..12324)
                     /locus_tag="OO135_004488"
     CDS             complement(10216..12324)
                     /locus_tag="OO135_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5400009.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12311..13132)
                     /locus_tag="OO135_004489"
     CDS             complement(12311..13132)
                     /locus_tag="OO135_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5400010.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13292..15121)
                     /gene="glmS"
                     /locus_tag="OO135_004490"
     CDS             complement(13292..15121)
                     /gene="glmS"
                     /locus_tag="OO135_004490"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5400011.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRIGHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTELLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15283..16653)
                     /gene="glmU"
                     /locus_tag="OO135_004491"
     CDS             complement(15283..16653)
                     /gene="glmU"
                     /locus_tag="OO135_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5400012.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILMF
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17003..17422)
                     /gene="atpC"
                     /locus_tag="OO135_004492"
     CDS             complement(17003..17422)
                     /gene="atpC"
                     /locus_tag="OO135_004492"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5400013.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17443..18825)
                     /gene="atpD"
                     /locus_tag="OO135_004493"
     CDS             complement(17443..18825)
                     /gene="atpD"
                     /locus_tag="OO135_004493"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5400014.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18852..19715)
                     /gene="atpG"
                     /locus_tag="OO135_004494"
     CDS             complement(18852..19715)
                     /gene="atpG"
                     /locus_tag="OO135_004494"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5400015.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19766..21307)
                     /gene="atpA"
                     /locus_tag="OO135_004495"
     CDS             complement(19766..21307)
                     /gene="atpA"
                     /locus_tag="OO135_004495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5400016.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21320..21853)
                     /gene="atpH"
                     /locus_tag="OO135_004496"
     CDS             complement(21320..21853)
                     /gene="atpH"
                     /locus_tag="OO135_004496"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5400017.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21868..22338)
                     /gene="atpF"
                     /locus_tag="OO135_004497"
     CDS             complement(21868..22338)
                     /gene="atpF"
                     /locus_tag="OO135_004497"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5400018.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAYKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22400..22639)
                     /gene="atpE"
                     /locus_tag="OO135_004498"
     CDS             complement(22400..22639)
                     /gene="atpE"
                     /locus_tag="OO135_004498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5400019.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22686..23501)
                     /gene="atpB"
                     /locus_tag="OO135_004499"
     CDS             complement(22686..23501)
                     /gene="atpB"
                     /locus_tag="OO135_004499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5400020.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23510..23890)
                     /gene="atpI"
                     /locus_tag="OO135_004500"
     CDS             complement(23510..23890)
                     /gene="atpI"
                     /locus_tag="OO135_004500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5400021.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(24507..25130)
                     /gene="rsmG"
                     /locus_tag="OO135_004501"
     CDS             complement(24507..25130)
                     /gene="rsmG"
                     /locus_tag="OO135_004501"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5400022.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQRPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(25194..27083)
                     /gene="mnmG"
                     /locus_tag="OO135_004502"
     CDS             complement(25194..27083)
                     /gene="mnmG"
                     /locus_tag="OO135_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5400023.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALDGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSHRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(27462..27905)
                     /gene="mioC"
                     /locus_tag="OO135_004503"
     CDS             complement(27462..27905)
                     /gene="mioC"
                     /locus_tag="OO135_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5400024.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWVNLLK"
     gene            complement(27995..28453)
                     /gene="asnC"
                     /locus_tag="OO135_004504"
     CDS             complement(27995..28453)
                     /gene="asnC"
                     /locus_tag="OO135_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5400025.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            28605..29597
                     /gene="asnA"
                     /locus_tag="OO135_004505"
     CDS             28605..29597
                     /gene="asnA"
                     /locus_tag="OO135_004505"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5400026.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(29602..31053)
                     /gene="viaA"
                     /locus_tag="OO135_004506"
     CDS             complement(29602..31053)
                     /gene="viaA"
                     /locus_tag="OO135_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5400027.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEKPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQLFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(31047..>32158)
                     /locus_tag="OO135_004507"
     CDS             complement(31047..>32158)
                     /locus_tag="OO135_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3763 domain-containing protein"
                     /protein_id="HCR5400028.1"
                     /translation="AINERQFRNGAHVEKIPMRLLVAASNELPEADSSLEALYDRMLI
                     RLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKEIGEITLPDHVFEL
                     IFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVDLILLKDCLWYDAQ
                     SLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKTALTVIRLGGIFSR
                     RQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLSKGGEIRGKLNCIG
                     FAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQLEELESDWRKQHAL
                     FSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         8192 a         8135 c         7448 g         8383 t
ORIGIN      
        1 aaagagccag tcagttgctt aactgactgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaatctcaa catggaacga tctagcctct
     3121 atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc
     3181 gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt agagacacaa
     3241 acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct cggcggcatt
     3301 gacctgttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat gtactggtac
     3361 tggttctcgg gagcacagga tgacgcctaa caattcattc aagccgacac cgcttcgcgg
     3421 cgcggcttaa ttcaggagtt agagagctgg ggaagactat gcgcgatctg ttgaaggtgg
     3481 ttctaagcct cgtacttgcg atggcatcgg ggcaggcact tgctgacctg ccaattgttt
     3541 tagtggatga agctcgtctt ccctatgact actccccatc caactacgac atttctccaa
     3601 gcaactacga caactccata agcaattacg acaatagtcc atcaaattac gacaactctg
     3661 agagcaacta cgataatagt tcatccaatt acgacaatag tcgcaacgga aatcgtaggc
     3721 ttatatatag cgcaaatggg tctcgcactt tcgccggcta ctacgtcatt gccaacaatg
     3781 ggacaacgaa cttcttttcc acatctggca aaaggatgtt ctacacccca aaaggggggc
     3841 gcggcgtcta tggcggcaaa gatgggagct tctgcggggc attggtcgtc ataaatggcc
     3901 aattttcgct tgccctgaca gataacggcc tgaagatcat gtatctaagc aactagcctg
     3961 ctctctaata aaatgttagg cctcaacatc tagtcgcaag ctgaggggaa ccactagtgt
     4021 catacgaacc tccaagagac ggttacacaa acgggtacat tgttgatgtc atgtatgaca
     4081 atcgcccaag taagtatcca gctgtgttca gaacgtacgt ccgaattcca gactatgtcg
     4141 aactagataa cgcggaggag ctggccgacg caatcattcg agaggttgag ttaaaggagg
     4201 catggctcac aagggtcttg gggaactcag ccgatcctga cgcttctgtg ggcaagtggg
     4261 accggcgcct agcgcagtgc ggtggtattg atgagatgtc aagtggggtg caggcaacat
     4321 acttcagtcc aaacggtcag ctggtcggct tagtggcata cattgatgcc gaatggatgg
     4381 atgaatagct ctgacgttac gattcaggtg agacaaccag atgggcattg cgtacaagct
     4441 cagttgccac cgatgtggct acgagtctga tcttctttac ctaggccaag gtatggcgat
     4501 gcttcctgag caaatagtcg gaacgtgcac atgctgcgac aatctaacga cgatttctat
     4561 aaatgaaagc tacggagtat gctcactttg tggggctaag gatcgtgtga tatttacaag
     4621 ctatcagcat gaggcaaggc caagaaatcc ctgctatcta gagagaagtt tcaaatatca
     4681 atgcccaaga tgccatgcct tcagtatgga gcctccggat ctaccggaga tgctttggga
     4741 ctaatgctcc gctttgcctc gggtatccgt tcgacctatg tttaagagga aacgcacgtg
     4801 tctgccgcca aagctcttgc ctatgcactt tgtgacaagt tggaaagcaa aaaagttgat
     4861 gaagtcgtac gaaatcttgt ttctagcggc tggaatatta gggaagtggc ctgggaagct
     4921 tcgtcctttg atcatgaaat gccctacttt gacacggggc gggacttcga tattggattt
     4981 cctgacacgc tgtcatccaa gattgagttt ttgaccgcga atggatcaat cgtccaagca
     5041 cgtgctcgcc tgcacttcaa gcaggctcta atttttgcca aggcgtccaa acatgtcgcc
     5101 gcgctcaaga actcattgga ttactactat ggatcgtctg taatcccccc tcaaaaccac
     5161 ggaggctatt tgtagaaaat tccattaata tgaactcaag gtgttcccta cggacgaatc
     5221 agcgatgaag gtggtgtacc tggctatcca ggcggcgacc aagaaatgga cgatgccgat
     5281 ccgcaactgg aagccggcca tgaaccgttt tatgatcgaa tttggtgacc gtttaaatgg
     5341 ccacctttaa ctgaccggca aaagcactta cacaaaaggg tttacaggct ctttcagtca
     5401 ttcctttaat gcgtaaattg ctctctaacc actctctcag cccacccttc catatcctct
     5461 ctcgatactt gcagctcccc caaccccttt ggatgattca cccctctatg cccgtcttgt
     5521 ccatataatt gatcaaacaa actatttggc caattcaatg agctcttcac cactccacgc
     5581 cgtatctttt caaaatcatt ccgccatgtt tcgctatcaa cgccactcaa cactttggta
     5641 gaaagcattt tcaccccatc cgatgcatcc acctctagca atacaaattc actgccatta
     5701 cgttgcaccc caacggcttt aatcactctt ggtgagccat ctttacacag atgtaatcgg
     5761 ctacgcccaa cttttggcaa aaccaaggtt tcttcaaaaa gcacccgaca tgcaaattta
     5821 gtttttagaa ttgaaagtag ctcatcaaaa gcagcaaatc gattagcaaa aatagagttg
     5881 taattggttg catcctgctt cccacccaca tctgccgctg ctaggacacc acctaagtgt
     5941 ggctcatcag tactaaccaa attactattt tcacttgttg ttgcttcttc tttttctaaa
     6001 atagtttggc tactttttct tgccctgcga gatttttcaa ttcgactagg tctactaaaa
     6061 cttatccatg tcggctcagc ctctatgact cgtgtgtctg tgtctatatt ggctgtttcc
     6121 tcatcgtcca actgtagatg ctcatcatca acaactgact ctgttgaaac cgctatgtgc
     6181 tgcgtactac catcacctgc ctccttttcc tgaaaagagg catggctaat agctgttgtg
     6241 ctaggaagca tagcgttgat ttctaaacct actatttcct caactaaata atccttatcc
     6301 tcgttagaag aacgtccttt tacatgtaat ttccaaccct gcatgggcgg agggtcaaag
     6361 ctaaagcacc agctttcaac gccgttctta atttctctat tttgttgata gtgcctaaaa
     6421 atactttcat acgaatccat aacatcttga tccgaaaaca accaaaccaa aagctgcact
     6481 acggccgact gctctaacgc ccctttagga aacgatgaac tgggtaagat ccttatctct
     6541 aaatgatctc gctcaacctc atactgaacg tcgaactctt gctgtaaagc ggtactgctc
     6601 aaacagcttc gacagaaata ggagttaatt aaaaataatg accgagctag ctctaactgt
     6661 gggatgtgat aacgagtctt gctatatggt gtttcagctt cgtaaataaa tgaactctgc
     6721 gctccatcct tgttgcgtac ggaagcaaag gacgagagat taggaaaatc gattattttg
     6781 gctcgttgaa gatccgatgc ttcaaactca atcacacgat ccgccttatt tatttgcttt
     6841 gttgtactgt taaccacctt agctcgactt aataaaggca aatgagtaaa tcgagtccat
     6901 ttccgttctt gcattggatt aaaccaaaca aaaatacgcc actccttatg acccgagtta
     6961 cggaataaat gaccaatatg tacaacctgc acattgtcat taaatgtagc tagcctaacc
     7021 actcactctt ccccataaac catttccaag aatctttgtg cttcctcagt tatccgctct
     7081 ttagataacg ttgcacttct taataatcgc cagcgcctaa gctcaacatc ttcctgttta
     7141 agcttaataa aagcttggct aatccgtcta atttgataat cttccacact ctctgagtaa
     7201 cgctttaagc aaagcgctac caaaggcagt ttctgtagat tttttgcaag agaggttccg
     7261 ttaggagttt gctttaacag ccagctcgat gtcgctcttg ggtgatcaag gctactatct
     7321 agacgcttta tgattcttaa tagttgtcgt acagcaattc gatctctttg gttccaatca
     7381 actctagggg cgggtgccag acgctcttgt tgatgctgtt gattccagtg aactagccaa
     7441 tccctgtcat gtcggtaaag ccaagcgtat agcaccccac cctctaaaga ctgtcttgcc
     7501 gccttaattc cttggtattt atgcactagt tgctgccagt ctttatgctt aacagataaa
     7561 tcttcagagt taggttgcac agactggcta acaggtcttg tcgttataga gtgctcagta
     7621 agagcacttg cctgctgtag cgcttctata accgttagtt ttggcaataa ggcttgccac
     7681 acaatactat gctgtaaata actaaaggct tttctatgct tacggaatat acttttcagc
     7741 caacacgtgt ccttgttctc tgccaacttt aaatccagtt tctctagtgc ctcatcacta
     7801 aaagtctgcc tcactctctc cgccaccaag tcatgacgaa tgtgcttgct tttggttagc
     7861 cctagatcct gcgctaagcg ctgataaaac agcgtccact gctcaaggct tggggaaagc
     7921 tcttgcgctc gtggagcatc taacagaggg gctatataag cagctagtgc tgttaattga
     7981 gatagggagt ctttggggta gtctgaaagc agctcagtat gacccaaagc ccaaaattga
     8041 tgtcggtgat catctacagc tctatcaaag aagactaaag caccgtgttt tggacaatat
     8101 ggcaaagcgg gcaaatacca atctcgttgc caaaaggctt ccccatacct atttagctga
     8161 agagcaacgc aatcagggca gtatctaaag cggttatcgc tcttaactct agaagcagcg
     8221 actcctagca ttaaatgcac cgcaccttgc gcttggtact ccattaaccg aatagcttcg
     8281 tctcggcgct ccttgcctac aaacggagca tataaaggga ataaggtatg ctcataaata
     8341 agctgctgaa cagcgtaacg tcctgtttga tgtagatgtc ttgctatcac acctaaatgc
     8401 gagggcagac ctaaggtagc gaccaccttg cggttgccat acacctcatc caacagctgc
     8461 ttaggactaa caatcccttg ataaacgcct gcccgtgcaa tagtgctata aatcagctca
     8521 ttcgagtacg gaacaggaaa gtttctcatg gctaacctgc ttgtttaaat aagctcgcca
     8581 tatccactat tacccctttc ccttttaacc gttcatgcat ggttttttca ggttggcgtt
     8641 gtgaatagat ataacgtaaa tccgtatcag gcaagctatc ccagtcgctg ggcttgacta
     8701 cctttatagc cgaaaccttt ttactcttgg agggcttttc tagttctgac actaccgttt
     8761 cgccttccat caaccactgc aaaacaagag gcagtaactt ttgtcttgtc atcgttggat
     8821 tctggctaaa cgctttttta atagtgggaa ttaacaggct tgaatcgtaa tcctctttca
     8881 gcatcagata taaatgacgc tgatcttcgg tatctaactc ttgaagggct ttttcttctg
     8941 gtgtttgttc ttgtatcgct gcgatatcta gctgaagttg gattaaccgt ttatcaatct
     9001 cgggaacgac tagatcagaa taacgagcaa tgcgttctgg gatacccgag cgtaatgcct
     9061 ctagcatggg gtgcacaggc tttaactcat cttgatacac ttgccgcaat aaaccagcgg
     9121 taatacgctc attgcctaaa gctagcgcac ggagctgagc gagtacaaaa agttttacta
     9181 caatgtccat cactccttgg cttagctcat accacacatc acggacctca tccgataaca
     9241 gcgcatcttt gcgttgtaaa agctgtaatt gccaaagatt atccgtaaaa gcgatccact
     9301 cttgattggg ctttccacgt tgcgtttgtt gtataggatc ccagaatata gctccaaacc
     9361 ctgcccctct acgtgcagac cgcaaatcag cctcaaaaat ctctcgtgct ttaggggtac
     9421 caatcaacat cactggtacg ccaataatat tcaccatcgt cacaaaaaag ttcagcatct
     9481 cttgagatcc acccgaacga gagcggctta aatgctgaat ttcatcaata accaacaacc
     9541 ctaaagcatg tgcattggct atttgcgaca tcaaagccaa catggtttct ataccatgac
     9601 gttttaagcc ataacgacgc tcatagttcg agcccaaggc tcgatccaac gctctgaaaa
     9661 aattcaagca gatttctttt agcgaaccat tatgcgagca gtctattttc aaatacacca
     9721 cctgctctac attgagttca cgatggtaaa tcacctgagg atacgtggct agaatacgat
     9781 gaagagaggt cgtcttccca ctaccagaac aaccaattaa caataagctt tgtgccgtag
     9841 atcgtgcctc ctcaaagcga aatgtctcca actctcccgt ttgaacacgc tcataaccat
     9901 tttgtaaatg cttttgtaaa tctcctgttt taggatttct gcctacgtag ccacctcgaa
     9961 tcatgaccga aatacgctca cttagtagca aatgcgtacc taatggctga aaatagtcat
    10021 ctggaatacg acaaatggta tgagctctga taacacggga cttttgcaag tcagaggaag
    10081 taagctgtaa agaggatttc agtgatgcag cactattcac tgactcttgt aatggtggta
    10141 aggcctcgat aaaaggatta tcacgataag cctctacccc cgtatcacga taaactgctt
    10201 gaatccgggt agcactcatg actcatcctt ttctggtgga tcctgaaata attcaggcac
    10261 gtatgtgggt aggctgtaat cttcttgatc atccgcttcc actgcgttga aaggaataac
    10321 tttagcctca tcacctgaag agcttggctt caaatgctcc gcacgttttt tacgctccga
    10381 ggtcacggct tcttttttat tagttttaat ctgcttaatg cgtgttgatt tgggctcagt
    10441 agtactgggc gttaacttat tcgctttctg aatggtttgc tgaataaacg cctcaagctc
    10501 cctgcgttta gttaactcat cctgcttcgc attggcttta ttgtgttttt cttgtgcttg
    10561 tatatcccaa acctcccaaa atgagagacc tttaaactgc cgactacgtt ccgtcagatt
    10621 acagcgccaa aatacacggc tgccaacttg cggaaacaaa taaatcgtat caaccagcac
    10681 tgggtcataa gccgcttcta aatgttgagg tctagctata tcagtgctcc gctgcaacca
    10741 accctcacgc agaatctctg accccgagta atacaaaccc cacaaattaa cgccaaatga
    10801 agaaatagag acctttcggc gaggcagtaa cgctactcgc aactgctctt gctccacagc
    10861 ccttaaacta cctgtacgat gctgcatacc ccattgccat agctggacag gaatagacgg
    10921 taaatctgta ggaaaatcag catctcgatc gtatttatcc atcaccagat ggttatttct
    10981 gaataagatc gtacgcaaaa taatttgtgt gaactcaaat accgacagag atgcatctaa
    11041 cctatagtct gtttcaccat ggcttttgat ccgactgccc tctacaatgc caggtgcaaa
    11101 ggacttaaac tcggcttgta gtgttctaaa agtgctttcc actatgcctt tagcatcgcc
    11161 acgtctaggt ggagcacttt ccactcgcac attaaaacta gaaactaagg cttcgacctg
    11221 atgactcatt aattcgccac ggtccgctag caacacatct ggcaaaccta cacacggcca
    11281 gtctgagcta ctaatctcaa tatcatgctg ggcacaaatg gccgttttgt cagagcaagc
    11341 atttacaaaa gcctgcatcg ccaccacata agacggattt tcaaagccga tataaaagcc
    11401 cgtgatcatc cgactaaaca catcaatcac aatgtaaagc gttggtcttc ctatgatttt
    11461 ttggcgatca tgatgatcca ctaaataaat atcagcaatc gtggcatcaa tctcataacg
    11521 actcccaggg cctaacgcct gagaagtggc tgtactactt aagggtcgta cgtctttttt
    11581 atatacccct gctttaactc tagactttaa gcgctgagct ttagggtatt ctcgatcata
    11641 aaaataacga aactgacgta gtgttgggta atcctcttgg ggaatgcgag gaaaatactg
    11701 agcaaacaag tccacaaatc gtctataggc aacggtggtc tttgtacctt tttgatttaa
    11761 caggtgcttt tctatggtca acctaaaaag gcgttcaatc tcgggcgtta ccttggttcc
    11821 ttcaccctta ccatattctc tggctcggcc aatctttgct gttcctgtcg ctgaacgtct
    11881 ttcccctggt gcaccgctgt ttttgtagtc aggaattaat gcattaggcg tttgaccacg
    11941 ctgccagtaa cggcgtaaca acttataaac tgtagcctta gtaaccttat gttcttggac
    12001 cacatgctca acgagttcgc ttctgacttt agggtcgaaa cgatccttac tattaataat
    12061 aggaagaatt tttcgataat cctcctcgcg cttctggaag ctgacagaat ctacagacgg
    12121 ctcttctaaa tcaagatgta cataagggtc atcagttcta actaagcggc cttcatctaa
    12181 atactgcatt agcaacaact cagccctagc ttgtggaact cctttatcag cgcttatttg
    12241 catccagaca acttggccat cctctatagc caaaatgcga tacggatcat tatcaaatag
    12301 cacaacctca ttaatttgcc acatagcgca actcctccat attcactact tggctaatac
    12361 agagatctgc acacttattt gccctgaaag acttataaat attgaactta ataaaaccat
    12421 ttgcggttaa ggctcgtatc tcactcaatg ttttgcctaa ctccaaatca taagcaatat
    12481 caacctgctt acagacattg ataatgtttt catctccttt ttcttgcagg atatgggcca
    12541 atggggatag ttgtgctaaa agctccgcag aaacttcttc tgttttcact gaataaagcc
    12601 attcaatatt ttcttttact acgggattta tttctttatc agtaaaaatg aaccaaggaa
    12661 tttgcttttg ctgccaatag cgacgctcta gttctagttt ttctaaggta cgctcgtctt
    12721 gtaaggctgc tgcaggtttg acttgaatag caaactgctc aaaaggacca tctttgcagt
    12781 ccactaaaaa atcagtagac ataacctgat ctacaccacg aataacagga tgcttaatac
    12841 cactatctat tgcaatctgc ctggtatcac taggtaataa ggggaactgc tcgcgtatat
    12901 ctagcacgct gctctcccac tcaagactga gaaaaacagc aagctctaag tcagatagca
    12961 aatgatggac tcgtcccgtc ttatgagaat aaatacggtg ggaacgacct gaagaaggaa
    13021 cttcttgtac tgttagccat ggaatatagt ctttaccatg cccttggcca cgcccctctt
    13081 taatacgacg ggcaatttgc acttcagaaa atgaagagtt tgctttagcc ataacaaaag
    13141 tccagtatgc tttttcacag cataactgga ctgatttcag tttacaacta ttctgtctag
    13201 tttaagactt tattgtcata gtttagatct attttgttca gtttaagact ttattgtccg
    13261 cccacaagcc cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaagt
    13321 tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca
    13381 gcggaacggt gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt
    13441 gcatgttatc gctacttaca aaacccgcat cctgatcggc gaagacatac aactgaccgc
    13501 cacgcgcgcg aacttcttca atgttggatt tcagtttttc caacagttcg ttgttcggtg
    13561 caacgacgat aaccggcata tccgcgtcaa tcagcgccag cgggccgtgt ttcagctcgc
    13621 cagcagcgta ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca
    13681 gtgcgattgg gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat
    13741 cttctgccag agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca
    13801 gcgcctgcag accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag
    13861 acagcttcgc caccagcatc aacagctcag ttaactgagt ggtgaatgct ttagtggacg
    13921 ccacgccgat ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag
    13981 aagaacccgg aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac
    14041 gcaggcccgc cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt
    14101 tacgacgcac ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa
    14161 tacctgccag cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac
    14221 aggcgaggat ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct
    14281 cgcttaaatc aacctgaccg tggccgatgc gcccggtaag ggtgtttttg atcgcgttcg
    14341 gctgttcgta gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat
    14401 attgcagatt ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt
    14461 ttaccgaacg gcgagtaatt tccgcaatat cgccctcttc aaggaagata aagcgacggg
    14521 tcaccggcag cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa
    14581 tcaccagcgg actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga
    14641 tcactgtacc gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag
    14701 tcccgccttg tttcagctcc cagttcacca gatgggcaat cacttcggta tcggtttcag
    14761 aaacgaaggt atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga
    14821 tgccgttatg caccaccata atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag
    14881 gttcaccgtg ggtcgcccag cgagtgtgag caataccggt gccgccatgc agaggatgtt
    14941 cttccgctgc ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac
    15001 cttccgcatc aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta
    15061 aaccttcaag aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca
    15121 tagtttttga ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg
    15181 ccccgagcct tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta
    15241 tgttatctcc tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg
    15301 acgacgccag ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc
    15361 gacattacgc gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg
    15421 ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt
    15481 attcgcacca tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat
    15541 ttccgcatcg cccaggtaag tcagatgacc agctttcgag cctttaccca gacgcgcttt
    15601 tttcatctca acaaagttac cgacgtgtgc accttccagc aactcagcac caggacgcag
    15661 acgggcaaac gggccaatag tacaggccgc tgccagattc acgtcttcca caacggtata
    15721 cggactgatt tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat
    15781 tttcacgcga tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac
    15841 atcgcgcccg tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac
    15901 gcctgctaac agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag
    15961 ttgcaggcgg ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat
    16021 ttcacgccct tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc
    16081 attattgttg gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat
    16141 gccggtgttg atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat
    16201 gccggtaact ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt
    16261 caccgtcagc agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc
    16321 gacagagatc agcggcacgt cgccgtagaa cattaaaatg tcttcatcat cggcaaagaa
    16381 aggtgcggcc tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt
    16441 aaggttgtcg tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaggtg
    16501 aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc
    16561 ggcaagggta tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc
    16621 aaggatcact acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg
    16681 aatttaaacc gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga
    16741 taaattgttc aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac
    16801 gccttttgtc tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt
    16861 gctcttattt cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct
    16921 atgtgaacgc tcttcaggac ggatcacgcg taaaaaaaac cagcctgttt ccagactggc
    16981 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
    17041 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
    17101 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
    17161 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc agactgcact tcaagaatgc
    17221 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
    17281 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
    17341 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
    17401 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
    17461 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
    17521 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
    17581 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
    17641 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
    17701 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
    17761 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
    17821 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
    17881 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
    17941 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
    18001 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
    18061 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
    18121 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
    18181 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
    18241 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
    18301 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
    18361 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
    18421 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
    18481 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
    18541 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
    18601 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
    18661 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
    18721 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
    18781 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
    18841 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
    18901 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
    18961 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
    19021 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
    19081 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
    19141 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
    19201 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
    19261 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
    19321 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
    19381 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
    19441 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
    19501 tcgcggtctt ccaggtaagg gtgcttatat tccagattac catgtgcaag gtgaccaatc
    19561 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
    19621 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
    19681 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
    19741 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
    19801 tgcctttcag tttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
    19861 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
    19921 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
    19981 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
    20041 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcta
    20101 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
    20161 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
    20221 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
    20281 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
    20341 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
    20401 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
    20461 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
    20521 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
    20581 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
    20641 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
    20701 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcacgc tggttgatga
    20761 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
    20821 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
    20881 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
    20941 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
    21001 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
    21061 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
    21121 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
    21181 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
    21241 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
    21301 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
    21361 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
    21421 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
    21481 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
    21541 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
    21601 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
    21661 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
    21721 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
    21781 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
    21841 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
    21901 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
    21961 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
    22021 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
    22081 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
    22141 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatatg ctcgttctgc
    22201 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
    22261 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
    22321 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
    22381 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
    22441 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
    22501 gaatcagatc aggttgacgc gctgcgcctt ccaagaattt acccccgagg atgccgatac
    22561 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
    22621 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
    22681 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
    22741 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
    22801 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
    22861 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
    22921 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
    22981 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccaat
    23041 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
    23101 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
    23161 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
    23221 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    23281 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    23341 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    23401 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    23461 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    23521 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    23581 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    23641 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    23701 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    23761 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    23821 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    23881 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    23941 ctttctttaa ggcttagagt caagcatcaa aaagaggtca aattatacgg cgcgcccccg
    24001 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    24061 acaattgcga cttttctgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    24121 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    24181 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cactctaaaa acagatactg
    24241 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    24301 gcagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    24361 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    24421 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagta taaccggtca
    24481 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    24541 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    24601 ggcaacaaag cgatttcatc ttccggcctt tgccctttca gcgcgtagaa acggccttgc
    24661 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    24721 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
    24781 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    24841 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    24901 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    24961 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    25021 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    25081 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    25141 tgttcttgat gcgttgcctg gtaagcgggt gcttaccaga catttttaat gcgttatgcg
    25201 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
    25261 gtgacgccag aaatacgcga agcttggccg atagaggctg gtttgtgatc gttaagtttg
    25321 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    25381 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    25441 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    25501 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    25561 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    25621 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    25681 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    25741 aaacgcagat ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac
    25801 atacgatacg gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac
    25861 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    25921 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    25981 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    26041 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    26101 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    26161 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg
    26221 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    26281 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    26341 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    26401 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    26461 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    26521 cgcaaacggt gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
    26581 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    26641 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    26701 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    26761 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    26821 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    26881 ccgtcgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    26941 ttgcagctca tctgtcccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    27001 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    27061 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    27121 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    27181 gtattgaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    27241 cgcactgccc tgtggataac aaggatccgg cttttaagat caacaacctt gaaaggatca
    27301 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    27361 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt
    27421 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaacc
    27481 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    27541 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    27601 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    27661 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    27721 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    27781 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    27841 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    27901 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    27961 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatagta cgcatgatcg
    28021 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    28081 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    28141 agtggcctgt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    28201 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    28261 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    28321 tctctacccg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    28381 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    28441 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    28501 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat atgtgtcggt
    28561 ttttattgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    28621 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgtcaa ctggaagaac
    28681 gtctggggct gatcgaagtc caggcaccga ttcttagccg tgtgggggat ggcacgcagg
    28741 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    28801 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    28861 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    28921 tttctccgtt gcactcggtc tatgtagacc agtgggactg ggaacgcgta atgggcgacg
    28981 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    29041 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    29101 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
    29161 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    29221 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    29281 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    29341 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    29401 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    29461 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    29521 aactgccgca tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg
    29581 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    29641 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    29701 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    29761 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    29821 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    29881 gccacgaaac agctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    29941 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag
    30001 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    30061 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    30121 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    30181 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    30241 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    30301 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    30361 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    30421 ttccggcttt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    30481 aagctggccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    30541 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    30601 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    30661 gcgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    30721 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    30781 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    30841 ggtgagttct ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
    30901 gggaacatca tcagtgattg ctgccttcag tcgtgggaat ttttcaaaga agaccgccag
    30961 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    31021 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    31081 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    31141 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    31201 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    31261 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    31321 tgggcaaagc cgatacagtt cagtttgccg cgaatttcac cacctttact cagccactgt
    31381 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    31441 ttttgcagca gcagagtcag agtggaagca gtaacgttaa cagggagttg atactgctga
    31501 cgacggctaa aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    31561 tgctgaagtt gcaggtgacg ttgcacaatc gcgcccaggc gggtcaacat cccttgctgt
    31621 tgccaggcgt gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    31681 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    31741 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    31801 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    31861 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    31921 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    31981 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    32041 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    32101 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcgg
//
LOCUS       DAJTMK010000468        12402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.123, whole genome shotgun sequence.
ACCESSION   DAJTMK010000468 DAJTMK010000000
VERSION     DAJTMK010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12402
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.123"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            <1..652
                     /locus_tag="OO135_004508"
     CDS             <1..652
                     /locus_tag="OO135_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5400029.1"
                     /translation="HPIIRGTNKVTGQSILQRHIASFSHHGLPQGSDDALRYIAEHDD
                     ALAFACINRQMIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNG
                     LVIIPGSRGDYSWIVQPVANEKTLHSLAHGAGRKWGRTECKWRLAAKYTVTQLSRTEL
                     GSRIICRDKQLIFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKSSGGKKG"
     gene            649..1263
                     /locus_tag="OO135_004509"
     CDS             649..1263
                     /locus_tag="OO135_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5400030.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO135_004510"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="OO135_004510"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5400031.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="OO135_004511"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="OO135_004511"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5400032.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="OO135_004512"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="OO135_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5400033.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="OO135_004513"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="OO135_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5400034.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPALEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="OO135_004514"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="OO135_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5400035.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTGNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="OO135_004515"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="OO135_004515"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5400036.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPGG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="OO135_004516"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="OO135_004516"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5400037.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNVSTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="OO135_004517"
     tRNA            9156..9231
                     /locus_tag="OO135_004517"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9397..10611
                     /locus_tag="OO135_004518"
     CDS             9397..10611
                     /locus_tag="OO135_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5400038.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIIIGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSDQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDALEKE
                     ENVVHGSFGKRA"
     gene            10754..11635
                     /locus_tag="OO135_004519"
     CDS             10754..11635
                     /locus_tag="OO135_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5400039.1"
                     /translation="MVRAKKQDLAWFDISNYEFLNDLTLPDLIQELEWRDFLLRHAKD
                     DTAIFKEEYDIKYERIFSGDPNLTILNEEEEEVEEFIRKVNDEAPSLRNEYDDLPSLS
                     SAMGVSPVTFSELAMYSFSSIDQGFFKRDEEDCYLKANAMLASVTGNLSNCSPNIILV
                     SIDLDDATDDEIITSLTHLLPLWRKELKVPEREHVAQKRIGLKTLQKLISNRVIPIVD
                     LLIWGEKSGKEVSNPMISALVFSDDPKDTQAIKESIKPFALESISERYTRLLQLYVNK
                     DREMSLIKISDLMSRDL"
     gene            11833..12030
                     /locus_tag="OO135_004520"
     CDS             11833..12030
                     /locus_tag="OO135_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5400040.1"
                     /translation="MSQSFIRLSEVQRRTGYSKVWIYRLIKEQRFPQSVKIGSRSIAF
                     IESEIEEWINQRIAESRGEVA"
     gene            12030..12365
                     /locus_tag="OO135_004521"
                     /pseudo
     CDS             12030..12365
                     /locus_tag="OO135_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3189 a         2916 c         3190 g         3107 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttc tcgcatcatg gtttgcctca aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgtg tattaatcgc cagatgatcg ctttgcgcat
      181 aatgcaacaa gttaaggcca cgggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcacg tggtgattac tcctggattg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggca gggcgtaaat gggggcgcac
      421 cgagtgtaaa tggcgtctgg cagcgaaata cacggtgacg caactctcgc ggactgaact
      481 tggcagtcgg ataatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggcc ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaag cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggac
      721 aggctaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg tggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggag
      961 caatccgatg cgatccggta tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgtcttgtt gaacaggcgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt
     1681 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
     1741 agggaggttt caaccacacc tttggctaca tcggagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc agtgcgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccaa aagcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc ggaccgtgaa ccacgttttc gtcagccaga
     2401 accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccgg tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgagagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tagttattga
     3301 tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tattccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcaggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgtt ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc tcttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt gacccacgtg
     3781 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc gacgatgatt
     3841 tatacgatac tgtgattggc taccgtggtg gcaactggat ttatgagtgg gccacccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgacact
     3961 ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgtga
     4261 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc tgcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaattttgc caatttggta
     4861 aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ctggaagtgc
     5041 gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagct
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagatactg
     5161 aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgacgag ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgca tcacatccgg caatatctat taaaactgat
     5341 acgtcatccc aatcgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaatgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattaacc agatcttcac caccgatgcc ctcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgagtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgccggtct gcgccgagga gtcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc tgtgcagtat
     6121 cactctctgc tttattaccg gcaaactctg cttcccaacg accataacca gtcagttgat
     6181 cgttaatttg tgtttcgcct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tgtcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttgtt atatatttct gcggcctgta cggatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aaaaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catatcgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcact tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggggggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gtcgcgatat cgcccacggt gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc cgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacctgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgt ctctacgcgt ttcaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgagcgta aataaaaccg ggtgatgtaa
     9061 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attcaaacat
     9241 ctccccaagt ctactaaagt ctttcaaaac cccttataat ccgcgtatta aagccccgtt
     9301 cgtcttttga cgcctactaa agtccctcaa aatctacggt cagatggggg tacttatggg
     9361 ggtatttgct gttcggttta gtggaggtac ccccaagtga aactcaatgc ccgtcaaata
     9421 gacacagcca agccaaaaga gaaggcttac aagctggccg atggtggcgg tttgtatctc
     9481 ttggtaaaac ctaatggagg taaatactgg cgacttaaat atcgtgtagc tggtaaagag
     9541 aagctattgg cactaggtgt gtatcctgag gttactctag ccgatgctcg ggcaaaacgt
     9601 gaagatgcga aaagaggtat cgctggtggt atcgatccga tggaagcgaa acgagaggaa
     9661 aagattgccc gggaaacgca gttaaacaac accttcaaag atattgccct tgagtggcac
     9721 agcagcaaat taaaaaaatg gtctgctggt tatgcttcag acatcctcga agccttcaac
     9781 aaagatgtgt tcccttacat tggcaaaaaa ccaatcgccg aaatcaaacc acttgaactt
     9841 ctgaatgtgc tgcggcgcat cgaggggcgc ggtgctacag aaaaagccaa aaaagtgagg
     9901 cagcggtgcg gggaagtttt ccgctatgca atagtcactg gtcgcgcaga gtataaccct
     9961 gcaccagacc ttactagcgc gatgcaaggc catgaatcta atcattaccc tttccttaca
    10021 gctaaagaat tacctgattt tttcaaggca ttgtccagtt actcaggaag cgcattggtt
    10081 gttatggcgg ctcgtctact gattatcatc ggcttgcgga ctggcgaact acgtggcgca
    10141 ttatgggatg aaattgattt caacaaggct atctgggaga tacccgcttc acgtatgaaa
    10201 atgcggcggc ctcatattgt gccattgtct gatcaggctc tttcgcttat tgggaagatt
    10261 agagaaataa caggcaatta ccctcttatg tttcccgggc gcaatgatcc aaggaaaaca
    10321 atgagtgagg ccagcataaa ccaagtgttt aagcgcattg gctacgctgg acgtgtaact
    10381 ggtcatgggt tccggcacac tatgagcacg attttgcatg agcagggcta taacaccgcg
    10441 tggatagaaa cgcagctcgc tcacgttgat aagaactcaa ttcgtggcac atacaaccat
    10501 gcgcaatatc tggatggaag gcgggagatg cttcaatggt atgccgacta tatggatgcg
    10561 ctggagaagg aggagaacgt tgttcatggt tcgtttggga aacgagctta actgtatgaa
    10621 tagaccgtgt taagcaatgg tagtagactt atgtagacga acaaagaata ggctatgtct
    10681 aggctgatcc ccgaaaaccc gtacacccct acgggctggc atagccgtca gaattagggg
    10741 cgcggggtgg cgtatggtga gagccaaaaa acaagattta gcatggtttg atattagtaa
    10801 ttatgaattt ttaaatgatt taaccttgcc ggacttgatt caagaattag agtggcgcga
    10861 ttttttattg cgtcatgcaa aagatgacac tgctatattc aaagaagagt acgatataaa
    10921 atatgaaagg attttttcgg gcgatcctaa cctaaccatt ctcaatgaag aagaggaaga
    10981 ggtcgaagaa tttattcgca aagtaaatga tgaagcccca tcattaagaa atgagtacga
    11041 tgatctaccc tcattatctt cagctatggg ggttagtcca gtaacatttt cagagctggc
    11101 catgtactct ttctcatcga tagatcaagg cttttttaaa agagacgaag aggattgcta
    11161 cctcaaggca aacgctatgc ttgcgagtgt tactggaaac ctaagtaatt gttcccctaa
    11221 catcatactc gtttctatag atttagatga cgccactgat gacgaaatta ttaccagcct
    11281 gactcacctt ctacctctat ggcgaaaaga gttaaaagta ccagagagag agcatgtcgc
    11341 tcagaaaagg attggattaa agacccttca gaagttgata tctaatagag tgatacctat
    11401 tgttgatctg ctgatttggg gtgagaagtc aggtaaggaa gtgagcaatc cgatgatttc
    11461 tgccttggtt tttagtgatg atccgaaaga tacgcaggct attaaggaat caataaagcc
    11521 ttttgcactt gaatctataa gcgagagata cacccgcttg cttcagcttt atgtaaacaa
    11581 agacagagaa atgagcttga taaaaatatc tgatttaatg agtagagatt tatagcaagc
    11641 aaaatcctac gtaaacgttt taagtaaatt tcgcgatttc cgctttgaat acgctactgt
    11701 gtatttcaaa gcggataatc ttctcaaaaa aatattttat ttttatccgc tatcacccat
    11761 tcaccggttt gtttaatctc ttttctgtct actgatgtcc attaaagaag atgacagaat
    11821 ataggagtaa acatgtctca atcatttatt cgtctttctg aggttcaacg ccgtactggt
    11881 tacagcaaag tgtggattta tcggctcatc aaagaacagc gattccctca atctgtaaag
    11941 attggaagcc gatcaattgc gtttattgaa agtgaaattg aagaatggat taatcagcgc
    12001 atcgctgagt cgcgtggcga ggtagcgtaa tgaataagaa aaaccgccca ttacaggcgg
    12061 ctaattcaga tattcgcgta tctgatgtta cgccccttac aaaatccctt caagcaccaa
    12121 agcgcacacc gaaaaagcat cgtgccagag tctatatgct gcgtactggt atagagggat
    12181 ggacagaaaa tgacattctt cgctactgcc gtctgtcttc tggtcgtaac tatgcaacag
    12241 agttagaacg ccagcttgga atcactctgg aacgtatcga cgaaaagaat cctgatggta
    12301 tcggaacaca ccttcgctac cgtttctcct gccgtggtga tgttctgaaa gtgatcactc
    12361 atatgtaagg cagtatgact ttccgtgaga cagatgctgg cc
//
LOCUS       DAJTMK010000469         9948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.145, whole genome shotgun sequence.
ACCESSION   DAJTMK010000469 DAJTMK010000000
VERSION     DAJTMK010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.145"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            complement(38..604)
                     /locus_tag="OO135_004522"
                     /pseudo
     CDS             complement(38..604)
                     /locus_tag="OO135_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(823..1800)
                     /gene="epmA"
                     /locus_tag="OO135_004523"
     CDS             complement(823..1800)
                     /gene="epmA"
                     /locus_tag="OO135_004523"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5400041.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGSETLAEVIAFSVDRA"
     gene            2125..3933
                     /gene="frdA"
                     /locus_tag="OO135_004524"
     CDS             2125..3933
                     /gene="frdA"
                     /locus_tag="OO135_004524"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5400042.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVPIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3926..4660
                     /gene="frdB"
                     /locus_tag="OO135_004525"
     CDS             3926..4660
                     /gene="frdB"
                     /locus_tag="OO135_004525"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5400043.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIEHD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4671..5066
                     /gene="frdC"
                     /locus_tag="OO135_004526"
     CDS             4671..5066
                     /gene="frdC"
                     /locus_tag="OO135_004526"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5400044.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5077..5436
                     /gene="frdD"
                     /locus_tag="OO135_004527"
     CDS             5077..5436
                     /gene="frdD"
                     /locus_tag="OO135_004527"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5400045.1"
                     /translation="MINPNPKRSGEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5499..6629
                     /locus_tag="OO135_004528"
     CDS             5499..6629
                     /locus_tag="OO135_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5400046.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
     gene            6718..7251
                     /gene="blc"
                     /locus_tag="OO135_004529"
     CDS             6718..7251
                     /gene="blc"
                     /locus_tag="OO135_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5400047.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7248..7565)
                     /gene="sugE"
                     /locus_tag="OO135_004530"
     CDS             complement(7248..7565)
                     /gene="sugE"
                     /locus_tag="OO135_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5400048.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7747..7893)
                     /gene="ecnB"
                     /locus_tag="OO135_004531"
     CDS             complement(7747..7893)
                     /gene="ecnB"
                     /locus_tag="OO135_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5400049.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(8004..8129)
                     /gene="ecnA"
                     /locus_tag="OO135_004532"
     CDS             complement(8004..8129)
                     /gene="ecnA"
                     /locus_tag="OO135_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5400050.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8181..8747)
                     /gene="efp"
                     /locus_tag="OO135_004533"
     CDS             complement(8181..8747)
                     /gene="efp"
                     /locus_tag="OO135_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5400051.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8789..9838
                     /gene="epmB"
                     /locus_tag="OO135_004534"
     CDS             8789..9838
                     /gene="epmB"
                     /locus_tag="OO135_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5400052.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
BASE COUNT         2554 a         2565 c         2522 g         2307 t
ORIGIN      
        1 catcatacac taaatcagta agttggcagc attacccgaa atgcccacca tttaataaca
       61 aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca ataccgccca
      121 ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg accagaatca
      181 tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca atacgccagt
      241 gctgggtcat gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcgtagg
      301 tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac
      361 gtggtccgac actattattc atccaggaat agataccacc ttcttctttg cgataagcag
      421 ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat
      481 aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa
      541 atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag
      601 gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg
      661 caacattgag caggcagcgt aaaaaccaat acagaattga tactcggatg atatcaaata
      721 tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca gaatggcgct
      781 tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc ggtcaacgct
      841 aaaggcgatg acttcagcca gtgtctccga gcccagcgcc aacatcacca gacgatcaac
      901 acctaatgcc acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg
      961 gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg
     1021 ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct caataccttt
     1081 ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga
     1141 tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc
     1201 tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg tatcagcaac
     1261 gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg
     1321 gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg ctgccgggca
     1381 gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg
     1441 cggtcgatac cactcaagca tagtgaactc agggttgtga taacgcccca tctcttcatt
     1501 acggaagctg cggcacagct ggaataccgg cccacaaccg gcaaccagca ggcgtttcat
     1561 atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggcctac
     1621 gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg
     1681 cgtctccacc tccagcactc cacgatcagc aaagaaacga cggatctccg ccataatcgc
     1741 cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat
     1801 agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca
     1861 aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg atctgatggt
     1921 ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc
     1981 gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag
     2041 ttgaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg
     2101 ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc
     2161 gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa
     2221 atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc
     2281 tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg
     2341 ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgcccaacc
     2401 gaaatgaccc aactggaact gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc
     2461 aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc
     2521 ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt
     2581 tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg cggcctggta
     2641 gcaatgaaca tgatggaagg cacgctggtg ccgatccgtg ctaacgcggt cgttatggct
     2701 actggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac
     2761 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
     2821 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc
     2881 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
     2941 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
     3001 caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg
     3061 gtttatctcg acttgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
     3121 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
     3181 ccgaccgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
     3241 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
     3301 ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
     3361 gagcgtgcag caactgccgg taatggcaac gaagaggcaa ttgaagcgca ggcagctggc
     3421 gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc
     3481 cgcgacgaaa tgggcctagc aatggaagaa ggttgcggta tctaccgtac gccggaactg
     3541 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc
     3601 accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt
     3661 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
     3721 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc
     3781 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
     3841 acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa
     3901 gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt
     3961 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
     4021 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
     4081 gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
     4141 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
     4201 agcgttagct aacttcccga ttgaacacga tctggtggtc gatatgaccc acttcatcga
     4261 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
     4321 taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg
     4381 tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc
     4441 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
     4501 tatggcgcag ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctattgctc
     4561 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
     4621 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
     4681 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
     4741 gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
     4801 ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
     4861 tacaaaaccc ggttatcgtg atcattaacc tgatcactct ggcggcagct ctgctgcaca
     4921 ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa
     4981 tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa
     5041 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
     5101 tctggcgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
     5161 gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat
     5221 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
     5281 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
     5341 gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
     5401 acagttgtca cgctgattgg tatcgttaca atctaacgta tcgccaatgt aaatccggcc
     5461 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
     5521 ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg
     5581 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
     5641 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
     5701 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
     5761 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat
     5821 actggcctga acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct
     5881 acaccgctgg cggcctgcca ttgcagatgc cggatgaagt gaaatcctca agcgacttgc
     5941 tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca
     6001 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
     6061 aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
     6121 cgcccgcaga agaaaagaat tacgcctggg gatatcgcga aggcaaggca gttcatgttt
     6181 cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc
     6241 gctgggtgca aagcaattta accccccttg atatcaatga gaaaacgctt caacaaggga
     6301 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
     6361 aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac aataaaattg
     6421 cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta cgcgcatcat
     6481 gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt attccagaaa
     6541 aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
     6601 cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt
     6661 tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg
     6721 cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct
     6781 cctacgccgc cgcgtggcgt gacagtagtg aataatttcg acgctaaacg ctatctcggt
     6841 acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc
     6901 gcaacataca gtctgcgtga tggcggcggc ctgaatgtca tcaataaagg ctataaccct
     6961 gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc
     7021 gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt
     7081 gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc
     7141 tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg
     7201 gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg
     7261 agtttcagac caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta
     7321 gcggactcac cgagcagcac aatgccggtg atagccgcgc cgactgcgcc aatacccgtc
     7381 cacacggcat aagccgtccc tactggtaac gatttcatcg cccaggcaag tagcgccata
     7441 ctgacaatca tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa gccgtgggta
     7501 tatttcaggc caacggccca taccacttcc agcagaccag caataactaa gataatccag
     7561 gacatatcag gctccggaga attggggccg tccccggtga aaagacgcgt ttgcaggtcg
     7621 tcctgcaacg ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt
     7681 accttagtga ctatcgcaga aaaaacaaac ggcacgacac agctgtcgta ccgttattgc
     7741 ttattgctat tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat
     7801 gtcttcacca acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac
     7861 agaaaagatc gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt
     7921 tatgtttgcc tatagcaaat taataataga caacatcgcc aaaaatgacg gaaatctgat
     7981 gatttttcgg aagagaagaa aaattagctg gcagcgcgag agatggagtt gccgagatgt
     8041 ttgatgtctt cgccgaaacc gcgagcggtg ttacagcccg tgagcagcgt actggcaagc
     8101 aaaacaagaa cgataaggcg tttcatcatc tctgacctga ataagtgatg gtgcagcctg
     8161 caggccgcac cacaaccgca ttacttcacg cgagagacgt attcaccaga gcgggtatcc
     8221 actttgatga cttcgccgat ttgtacgaac agcggaacct taaccacagc gccagtagac
     8281 agggtagccg gtttgccgcc agtacctgcg gtatcacctt tcaggcccgg atcggtatca
     8341 acgatttcca gttcaacgaa gttcggcgga gtaacggaga tcggctgacc attccacaga
     8401 gttacgatac actctgcctg atccagcagc catttagcgt tatcgccaat tgcttttgca
     8461 tcagcagaca gctgctcgaa agtttcgttg ttcatgaagt gccagaactc accgtcgttg
     8521 tacaggtaag tcaggttcat atcgacaaca tcagcgcctt cagcggaatc agtagatttg
     8581 aaggtttttt ctacgcgagt accggtcagc agacgacgca gtttaacgcg agcaaatgcc
     8641 tggcctttac ccggttttac gaattcactc gcttcaaccg cgtaaggttc gccgtctaac
     8701 atgattttaa gaccagcacg aaaatcgttg ctatagtacg ttgccataag gccctctgaa
     8761 atttgttaat tggtagctaa gccacaaaat ggcgcatatt gtaaccctaa ataccccatc
     8821 cagagaagat tggttaacgc aacttgccga tgttgtgacc gatcctgatg aacttctgcg
     8881 tcttttgaat atagacgcgg acgaaaaact gttggccgga cgcagcgcca aaaagctgtt
     8941 tgccctgcgt gtgccccgct catttatcga tcgcatggag aaaggcaatc cggacgatcc
     9001 tcttttgcgt caggtactta cctcgcaaga tgagtttgtc gtcgcgtccg gattctccac
     9061 cgacccactg gaagaacagc acagcgtagt gcctggtttg ttgcataaat accacaaccg
     9121 ggcgcttttg ctggtcaaag gcggctgcgc ggtaaattgc cgctattgct tccgtcgcca
     9181 cttcccctat gccgaaaatc agggcaacaa gcgtaactgg cagactgcac ttgagtatgt
     9241 tgctgcgcat ccggaactgg acgagatgat tttctccggc ggcgatccgc tgatggcgaa
     9301 agatcacgag ctggactggt tgctcacaca actggaagcc atcccgcata tcaaacgtct
     9361 gcggattcac agccgtctgc cgattgtgat cccggcacgc atcacagatg cgctggttga
     9421 acgcttttca cattctacgc tgcaaatcct gctggtgaat cacatcaacc atgccaatga
     9481 gatagatgaa acattccgtc aggcgatggc taagttgcgc cgggtaggcg ttactttgct
     9541 gaaccagagc gttctgttac gtgatgtgaa cgataacgca caaacgctgg caaacctgag
     9601 taatgcgttg ttcgatgccg gcgtgatgcc gtattacctg catgtgctcg ataaagtaca
     9661 gggcgcggcg cattttatgg tgagtgatga cgaagcacgg cagattatgc gtgagttgct
     9721 gacattggtg tcgggatatc tggtgccgaa actggcgcga gaaattggcg gcgaacccag
     9781 caaaacgccg ctggatctcc agctacgcca gcagttactg attaaacagg gaagttaaac
     9841 attaaaattg cctgatgcgc tacgcttatc aggcctacat cgtgcattga aattgaatgg
     9901 atttgaaatg aactgtaggc cggataaggc gtttacgccg catccggc
//
LOCUS       DAJTMK010000470         8846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.154, whole genome shotgun sequence.
ACCESSION   DAJTMK010000470 DAJTMK010000000
VERSION     DAJTMK010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.154"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            260..919
                     /locus_tag="OO135_004535"
     CDS             260..919
                     /locus_tag="OO135_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5400053.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1120..1497)
                     /locus_tag="OO135_004536"
     CDS             complement(1120..1497)
                     /locus_tag="OO135_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5400054.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1564..4530)
                     /locus_tag="OO135_004537"
     CDS             complement(1564..4530)
                     /locus_tag="OO135_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5400055.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4533..5093)
                     /locus_tag="OO135_004538"
     CDS             complement(4533..5093)
                     /locus_tag="OO135_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5400056.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5219..5569)
                     /locus_tag="OO135_004539"
     CDS             complement(5219..5569)
                     /locus_tag="OO135_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5400057.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5772..6785)
                     /gene="intI1"
                     /locus_tag="OO135_004540"
     CDS             complement(5772..6785)
                     /gene="intI1"
                     /locus_tag="OO135_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5400058.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6995..7825
                     /locus_tag="OO135_004541"
     CDS             6995..7825
                     /locus_tag="OO135_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5400059.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7938..8729
                     /gene="aadA1"
                     /locus_tag="OO135_004542"
     CDS             7938..8729
                     /gene="aadA1"
                     /locus_tag="OO135_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5400060.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2014 a         2365 c         2496 g         1971 t
ORIGIN      
        1 catcatacac taaatcagta agttggcagc atcacccgac gcactttgcg ccgaataaat
       61 acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg ttgataccgg
      121 gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      181 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      241 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
      301 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      361 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      421 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
      481 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
      541 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
      601 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      661 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
      721 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
      781 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
      841 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
      901 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
      961 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac
     1021 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc
     1081 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt
     1141 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag
     1201 agcctgataa aaacggttag cgccggggtt ggatttttca gcgttccagc taaggctaag
     1261 gcattcctgt tcaagcgcaa gccgggctat aaagcgcatt atcgctttac ccgtgccctt
     1321 atttcgatcg cactgagaaa catacagctc tttaatatgc agctgaccgc tgtatcgggg
     1381 cgagggataa agaatattgc aacatgccag gccagtaatg ttattgccgc agcgcgctct
     1441 gatcaccagg gtaccggaaa gccggttgaa taacttttta cagagataat ccttcatcaa
     1501 cgcttcctga atgatacctt cgccataata ggggtcgtct cagaaaacgg aaaataaagc
     1561 acgctaagcc ggttgcagag gccgtagcgg cctgaacttc cccgcgccga tcttggcgct
     1621 gctgcgccat aggtaatcac cggtcaggtt gatgtgctcc cagccgagtg gcgacaggta
     1681 ctgcaatagc gagtcatcga cggcatgacc attgccgcgc aacgcatgcg ccgcacgctc
     1741 caggtagacc gtgttccaca gcacgatggc cgccgtcacc aggttgaggc cgctggcccg
     1801 gtagcgctgc tgctcgaaac tgcggtcacg gatttcacca aggcggttga agaacacggc
     1861 acgggccagc gcattgcgcg cctcgccctt gttcagcccg gcatgcacgc ggcggcgtag
     1921 ctcgacgctt tgcagccagt cgaggatgaa cagcgtgcgc tcgatgcggc ccaactcgcg
     1981 cagcgcgacg gccaagccgt tctggcgcgg gtagctgccg agtttcctga gcatcagcga
     2041 ggccgtcacc gtgccctgct tgatcgaggt ggccagccgc aggatttcgt cccaatgggc
     2101 gcggacgtgc ttgatgttga gcgtgccgcc gatcatcggc ttgagcgcgt cataggcggc
     2161 atcgcccttc gggatgtaga gcttggtgtc gcccaggtcg cggatgcgcg gcgcgaagcg
     2221 gaagcccaag aggtgcatca gggcgaagac gtgatcggtg aagcccgccg tgtcggtgta
     2281 gtgctcctcg atccgcaggt cggattcgtg gtacagcagg ccgtcgagca cgtaggttga
     2341 gtcgcgcagg ccgacattga ccaccttggt gtggaatggc gcgtattggt cggagatgtg
     2401 ggtgtagaaa gtccgtcctg ggctgctgcc atattttggg ttgatgtgcc ccgtgctctt
     2461 tgccttgcta gcggttcgga aattctgtcc gtccgatgat gatgtggtgc catcgcccca
     2521 gtgcccggca aagggatgcc gaaactgagc gttgaccagt tcagccaacg ctgtcgagta
     2581 cgtttcgtcg cgggtatgcc aggcttgcag ccaagcgagc ttcgcgtagg tcgtgccggg
     2641 gcaggactcg gccatcttgg tcaggcccag gttgatcgcg tcggccagga tcgtggtcaa
     2701 caacaggttc ttgtccttgg ccagatcgcc cgatttcaag tgcgtgaagt gccgggtgaa
     2761 gcccgtccac tcatcgactt cgagcagcag ttcggtgatc ttgacgtgcg gcaggaccat
     2821 ggctgtctgg tctatcagcg cctgcgcggt gtcgggcacc gccgcatcca gcggcgtgat
     2881 cttcaagccc gactcggtga tgatggcatc cggcaggtcg ttggctgccg ccatgcggtt
     2941 gacggtggca agttgtgctt ccagcagcgt cagccgctca tgcagatatt gttcgcagtc
     3001 ggtggccacg gccagcggca attcgctgga ctgcttgagg ctggtgaact tctcgggcgg
     3061 taccaggtag tcctcgaagt ccttgaactg gcgtgaaccc tgcacccaga tgtcgcccga
     3121 gcgcagggag ttcttcaact cggacagcgc gcacagttcg tagtagcgcc ggtcgatgcc
     3181 ggcgtcggtc atcaccagtt tctgccagcg cggcttgatg aagccggtcg gtgcatcggc
     3241 tggcagcttg cgggcgttgt cggtgttcat gccgcgcagc acctcaatgg catcaagcac
     3301 gtttttggcg gcgggcgcgg cccgcagctt gagcacggca aggaattccg gtgcatagcg
     3361 gcgcagggtg gcgtagctct cgccgatgcg atgcaggaaa tcgaagtcat cgggttgcgc
     3421 gagcttctgc gcctcggtga cgctctcggc aaaggaatcc caggacatga cggcctcgat
     3481 ggcggcaaac gcatcgcggc ctgattgctt ggcgtcgatc agcgcctgac cgatgcgccc
     3541 gtacagacgt accttggcgt tgatggcctt gcctgacgcc tggaactgct gctgatgctt
     3601 attcttggca gcgttaaaca gcttacccag gatgcggtcg tgcaggtcga tgatttcgtc
     3661 ggtgacggtg gccatgccct cggtggccag cgccacgaga gtggcgtagc gccgttgcgg
     3721 ctcgaatttg gccaggtcgg cgggtgtcat ctggccgccc tcgcgggcaa tcttgagcag
     3781 gcggttctgg tgaaccagcc gctcgatgcc ggtaggcaga tcgagtgcct gccatgcctt
     3841 gaggcgttcg atgtgttcca gcatatgccg cgaatttggc ttggccggag actggcgcaa
     3901 ccaagccaac caggtcgtct tgccgttgtc ccggcgcttg agcagatcgt cgaggcggcg
     3961 gcgatgcgcg tccgccagtg gttcggccaa ggcgtcgtag atgcgccggt tagcacgggt
     4021 gatcgcctcg gcactcgccc gctcgacggc gttgagggcg ggcagaatga ccgactgccg
     4081 ccgcaggtgc ccgatcaagg cgctggccag cacgatgcct ttgtcggttt gcatcgccag
     4141 ctcggtcagc atctggacgg cctgccggta atggctcatg gtgaagggcc ggaaaccgaa
     4201 cacggtttgc agctcgctca ggtgctcgcg ccgggtctgc tcccgctggc cgtactcgtt
     4261 ccagctttcg acgccgacct tgagctggtc ggcgaccagc ttcaacaagg gcgggaacgg
     4321 tagttcatcg acgcccagga tgacgccggg aaagcgcagg taacagagct gcaccgcgaa
     4381 gcccagccga ttggctggcc cgcgccgctg tcggatgatc gagaggtcgg tatcgttgaa
     4441 tgtgtaatgt cggatcaggt cgtccttgga gtccggcaac gccagcaggc tttcccgctc
     4501 ggcggcggac aggatggaac gacgtggcat atttactgat ccgttctcaa gtattgatac
     4561 agggtttcgc gactgattcc gaattcacga gcaagcttgg tcttttgctc gccagcctcg
     4621 acacgttggc gcagttcggc aatacgctca gacgacaggg atttcttcct gccacggtaa
     4681 gccccgcgtt gcttggcgag cgcaataccc tcgcgctgac gctcgcggat cagggcgcgc
     4741 tcgaactcgg cgaacgcgcc catcaccgag agcatcaggt tcgccatcgg agagtcttcg
     4801 ccagtaaaac tgaggtgttc cttgacgaat tcgatatgca cgccgcgttg tgtcagcgtt
     4861 tgcacgatcc ggcgcaaatc atcgagattg cgcgccaggc gatccatgct atgcaccacc
     4921 acggtgtcgc cggtgcgggc gaagcttatc agcgcttcca gttgcggacg cttgacatcc
     4981 ttgccggatg ccttgtcgct aaaagcgcga tcaaccttga cgccttccag ttgccgttcc
     5041 gggttctggt cgaaggtgct gaccctgata tacccaatgc gctgtccagt catggaattc
     5101 cctgcaaaat gtcagggaag actctatgac cttcaacgag atatgtcaat aaattcaaaa
     5161 ttcaatccta tcctgacgca atttacacat ggcatctgac atcaggttag ggtatgcctc
     5221 aacctgacgg cggcgaatca caagcgtccg gtttgacgct ggtttcggtc gcagtgctgg
     5281 cccgcgcctg gaagcgctga tagcactcca gcccgcagaa atgctccacg tactcggccc
     5341 cttccggcgt gaaggcggca tcgagcggga tttccttgca gcacacgcag caactggtgc
     5401 tcggttcatt ggcgttcatg gtggtgttcc tccattggtt gacgaagccg acgaaggccg
     5461 ccgccggcat cggcctggcg aacaggaaac cctgcaccgt gtcgcaaccc gcctgccgca
     5521 accacgcaag gcagtcgggt gtttccacac cctcggctac cacctccatt gtcgttttca
     5581 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
     5641 catcaggcaa cgacgggctg ctgccggcca tcagcggacg cagggaggac tttccgcaac
     5701 cggccgttcg atgcggcacc gatggccttc gcgcaggggt agtgaatccg ccaggattga
     5761 cttgcgctgc cctacctctc actagtgagg ggcggcagcg catcaagcgg tgagcgcact
     5821 ccggcaccgc caactttcag cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg
     5881 ccgagcagat cctgcacggt tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac
     5941 gagtggcgga gggtgtgcgg tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt
     6001 ttgaaggcgc gctgaaaggt ctggtcatac atgtgatggc gacgcacgac accgctccgt
     6061 ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc acggccagga atgcccggcg
     6121 cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg
     6181 tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc
     6241 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     6301 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     6361 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     6421 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     6481 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     6541 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     6601 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     6661 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     6721 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     6781 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     6841 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     6901 cagggcagtc gccctaaaac aaagttgggc gaacccggag cctcattaat tgttagccgt
     6961 taaaattaag ccctttacca aaccaatact tattatgaaa aacacaatac atatcaactt
     7021 cgctattttt ttaataattg caaatattat ctacagcagc gccagtgcat caacagatat
     7081 ctctactgtt gcatctccat tatttgaagg aactgaaggt tgttttttac tttacgatgc
     7141 atccacaaac gctgaaattg ctcaattcaa taaagcaaag tgtgcaacgc aaatggcacc
     7201 agattcaact ttcaagatcg cattatcact tatggcattt gatgcggaaa taatagatca
     7261 gaaaaccata ttcaaatggg ataaaacccc caaaggaatg gagatctgga acagcaatca
     7321 tacaccaaag acgtggatgc aattttctgt tgtttgggtt tcgcaagaaa taacccaaaa
     7381 aattggatta aataaaatca agaattatct caaagatttt gattatggaa atcaagactt
     7441 ctctggagat aaagaaagaa acaacggatt aacagaagca tggctcgaaa gtagcttaaa
     7501 aatttcacca gaagaacaaa ttcaattcct gcgtaaaatt attaatcaca atctcccagt
     7561 taaaaactca gccatagaaa acaccataga gaacatgtat ctacaagatc tggataatag
     7621 tacaaaactg tatgggaaaa ctggtgcagg attcacagca aatagaacct tacaaaacgg
     7681 atggtttgaa gggtttatta taagcaaatc aggacataaa tatgtttttg tgtccgcact
     7741 tacaggaaac ttggggtcga atttaacatc aagcataaaa gccaagaaaa atgcgatcac
     7801 cattctaaac acactaaatt tataaaaaat ctaatggcaa aatcgcccaa cccttcaatc
     7861 aagtcgggac ggccaaaagc aagcttttgg ctcccctcgc tggcgctcgg cgccccttat
     7921 ttcaaacgtt aaacatcatg agggaagtgg tgatcgccga agtatcgact caactatcag
     7981 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
     8041 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
     8101 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
     8161 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
     8221 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
     8281 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
     8341 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
     8401 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
     8461 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
     8521 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
     8581 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
     8641 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgttcac tacgtgaaag
     8701 gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc cgacgccgct
     8761 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgcg gtgatgctgc
     8821 caacttactg atttagtgta tgatgg
//
LOCUS       DAJTMK010000471         5663 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.225, whole genome shotgun sequence.
ACCESSION   DAJTMK010000471 DAJTMK010000000
VERSION     DAJTMK010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.225"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            67..282
                     /locus_tag="OO135_004543"
     CDS             67..282
                     /locus_tag="OO135_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5400061.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            275..661
                     /locus_tag="OO135_004544"
     CDS             275..661
                     /locus_tag="OO135_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5400062.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            669..1355
                     /locus_tag="OO135_004545"
     CDS             669..1355
                     /locus_tag="OO135_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5400063.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1333..1956)
                     /gene="tetR(B)"
                     /locus_tag="OO135_004546"
     CDS             complement(1333..1956)
                     /gene="tetR(B)"
                     /locus_tag="OO135_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5400064.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2038..3243
                     /gene="tet(B)"
                     /locus_tag="OO135_004547"
     CDS             2038..3243
                     /gene="tet(B)"
                     /locus_tag="OO135_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5400065.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3356..3949)
                     /gene="tetC"
                     /locus_tag="OO135_004548"
     CDS             complement(3356..3949)
                     /gene="tetC"
                     /locus_tag="OO135_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5400066.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4082..4453
                     /locus_tag="OO135_004549"
     CDS             4082..4453
                     /locus_tag="OO135_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5400067.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4463..>5663)
                     /locus_tag="OO135_004550"
     CDS             complement(4463..>5663)
                     /locus_tag="OO135_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5400068.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1569 a         1044 c         1230 g         1820 t
ORIGIN      
        1 gaacataaaa cactatcaat aagttggagt cattaccgac cactattaag tcatgtagct
       61 ggttttattt caattgaacg ttttcaaagt ctagctacag aaggaaaaat gttatcgcta
      121 tcttggtggg aaaacgaata cgcagttctg caatggaaaa atcatgtttt acatgcgaaa
      181 gctcaacaag aagggcgaga gtcaatattt gatttttaca aaattagtat tgctcatatt
      241 actcgcgaat attcatttaa aaaggacaag gataatgttt gatgttcacg ttgttttaga
      301 taatcaaata ggacaattag cattactagg aaaaacatta ggtaataaag gtattggatt
      361 ggaaggggga gggatattta cggttggtga tgaatgccat gctcattttc ttgttgaaca
      421 aggaaaggaa gctaaaatag cgctagagca agctggactg ttagtacttg cgatccggac
      481 accattaatt cgtaagttaa aacaggaaaa accgggggaa cttggcgaaa tagcacgagt
      541 attggcggag aataacatta atattttagt gcaatacagt gaccatgcta accaactgat
      601 attaataacg gacaatgata gtatggctgc atctgttacg ctcccttggg caataaagtg
      661 aacttgcgat ggctaattta atacgaaaag aggttacctt tgagtcctca atagccgcga
      721 taggggcggc tatgtctgac atttcacgag ttaaaatact cagtgctttg atggatgggc
      781 gagcttggac ggccactgag ctaagttctg tggcgaatat atcagcttca acggcgagca
      841 gtcatttatc taaattatta gattgccagc taatcacagt agtagctcaa ggcaagcatc
      901 gttattttcg gctagcagga aaagatattg ctgaattgat ggaaagtatg atggggatct
      961 ccttaaacca tggcgtacat gccaaagttt ccacgccagt gcatttacga aaagcacgta
     1021 cttgctatga tcatttagct ggcgaagttg ccgttaagat ctatgattcc ctttgtcaac
     1081 agcaatggat cactgaaaat ggttcaatga tcacattaag tggtattcaa tattttcatg
     1141 aaatgggaat tgacgttcct tccaaacatt cacgtaaaat ctgttgtgcg tgtttagatt
     1201 ggagtgaacg ccgtttccat ttaggtgggt acgttggagc cgcattattt tcgctttatg
     1261 aatctaaagg gtggttaact cgacatcttg gttaccgtga agttaccatc acggaaaaag
     1321 gttatgctgc ttttaagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
     1381 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
     1441 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
     1501 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     1561 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     1621 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     1681 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     1741 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     1801 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     1861 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     1921 gctgttaatc actttacttt tatctaatct agacatcatt aattcctaat ttttgttgac
     1981 actctatcat tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg
     2041 aatagttcga caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt
     2101 atcatgccag tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac
     2161 cactttggcg tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt
     2221 ggaaaaatgt ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca
     2281 tcgctggatt acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt
     2341 ttgctttcag ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc
     2401 acctcagctt ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt
     2461 ttaatagcgg ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt
     2521 tttatcgctg cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa
     2581 accaaaaata cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg
     2641 gtatacatca ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa
     2701 ttgataggcc aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg
     2761 aatagcatga tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa
     2821 gcctttgtgg caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgga
     2881 tttattgcag atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagtt
     2941 ttccctgttt taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg
     3001 atgtctatcc aaacaaagag tcatcagcaa ggtgctttac agggattatt ggtgagcctt
     3061 accaatgcaa ccggtgttat tggcccatta ctgtttgctg ttatttataa tcattcacta
     3121 ccaatttggg atggctggat ttggattatt ggtttagcgt tttactgtat tattatcctg
     3181 ctatcgatga ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct
     3241 tagttatttc gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc
     3301 aagagggcat tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt
     3361 tatctttcaa gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg
     3421 caagctcttt taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt
     3481 ttttaagcac atcatcatca taagctttaa gttggttctc ttggatcaat ttgctgacaa
     3541 tggcgtttac cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa
     3601 tgataggcat cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa
     3661 gataatgttc aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg
     3721 tgatgtaggc atcaatcata attaattgct gcttataaca ggcactgagt aattgttttt
     3781 tatttttaaa gtgatgataa aaggcacctt tggtcaccaa cgcttttccc gagatctcat
     3841 ctattgaaac agcttgatag cctttttcaa caaacaatat tcgtgctgag ttaaccagtg
     3901 attgataggt actcttaaaa ttttcttgtt gatgattttt attttccatg atagatttaa
     3961 aataacatac cgtcagtatg tttatggtat catgatgatg tggtcgtgac aatcttaaga
     4021 acatttaggt tattttatgt atattgaaca gcattctcgc tatcaaaata aagctaataa
     4081 catccaatta agatatgatg ataagcagtt tcatacaacg gttatcaaag atgttctatt
     4141 atggattgaa cataatttag atcagtcttt actgcttgat gatgtggcga ataaagcggg
     4201 ttataccaag tggtattttc agcggctgtt caaaaaagta acaggggtca cactggctag
     4261 ctatattcgt gctcgtcgtt tgacgaaagc ggctgttgag ttgaggttga cgaaaaaaac
     4321 tatccttgag atcgcattaa aatatcaatt tgattcccaa caatctttta cacgtcgatt
     4381 taagtacatt tttaaggtta caccaagtta ttatcggcgt aataaattat gggaattgga
     4441 ggcaatgcac tgagagatcc cctcataatt tccccaaagc gtaaccatgt gtgaataaat
     4501 tttgagctag tagggttgca gccacgagta agtcttccct tgttattgtg tagccagaat
     4561 gccgcaaaac ttccatgcct aagcgaactg ttgagagtac gtttcgattt ctgactgtgt
     4621 tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg aacgcccgca agccaacatg
     4681 ttagttgaag catcagggcg attagcagca tgatatcaaa acgctctgag ctgctcgttc
     4741 ggctatggcg taggcctagt ccgtaggcag gacttttcaa gtctcggaag gtttcttcaa
     4801 tctgcattcg cttcgaatag atattaacaa gttgtttggg tgttcgaatt tcaacaggta
     4861 agttagttgc tagaacccat ggctcctttg ccgacgctga gtagatttta ggtgacgggt
     4921 ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc tttagagcga gatttataca
     4981 atagaatttg gcatgagatt ggattgcttt tagtcagcct cttatagcct aaagtctttg
     5041 agtgactaga tgacatatca tgtaagttgc tgataggttt ccagttttcc gctcctaggt
     5101 ctgcatattg tacttttcct cttactcgac ttaaccagta ccaacccagc ttctcaacgg
     5161 atttatacca tggcacttta aagccagcat cactgacaat gagcggtgtg gtgttactcg
     5221 gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc tttctttgaa cattgctctg
     5281 aaagcgggaa cgctttctca taaagagtaa cagaacgacc gtgtagtgcg actgaagctc
     5341 gcaataccat aagtcgtttt tgctcacgaa tatcagacca gtcaacaagt acaatgggca
     5401 tcgtattgcc cgaacagata aagctagcat gccaacggta tacagcgagt cgctctttgt
     5461 ggaggtgacg attacctaac aatcggtcga ttcgtttgat gttatgtttt gttctcgctt
     5521 tggttggcag gttacggcca agttcggtaa gagtgagagt tttacagtca agtaatgcgt
     5581 ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa ttcggggtaa tgactccaac
     5641 ttactgatag tgttttatgt tca
//
LOCUS       DAJTMK010000472         4798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.247, whole genome shotgun sequence.
ACCESSION   DAJTMK010000472 DAJTMK010000000
VERSION     DAJTMK010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.247"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OO135_004551"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OO135_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5400069.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1483..2286
                     /locus_tag="OO135_004552"
                     /note="aph(3'')-Ib"
     CDS             1483..2286
                     /locus_tag="OO135_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5400070.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2286..3122
                     /locus_tag="OO135_004553"
     CDS             2286..3122
                     /locus_tag="OO135_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5400071.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSSAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3229..3633)
                     /locus_tag="OO135_004554"
                     /pseudo
     CDS             complement(3229..3633)
                     /locus_tag="OO135_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3677..4066)
                     /locus_tag="OO135_004555"
     CDS             complement(3677..4066)
                     /locus_tag="OO135_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5400072.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4284..4724
                     /locus_tag="OO135_004556"
     CDS             4284..4724
                     /locus_tag="OO135_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5400073.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1123 a         1299 c         1255 g         1121 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1561 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1621 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1681 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1741 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1801 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1861 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1921 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     1981 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     2041 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     2101 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     2161 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     2221 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     2281 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2341 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2401 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     2461 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2521 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2581 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2641 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2701 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2761 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2821 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     2881 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     2941 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     3001 tgctcgacca ggcgtacgct tatgggtgcc tttcctcagc ttggaacgcg gatggagaag
     3061 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     3121 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     3181 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgaccccaa acaggaaacg
     3241 cagctgaaac gggaagctca acacccactg acgcatgggt tgttcaggca gtacttcatc
     3301 aaccagcaag gcggcacttt cggccatccg ccgcgcccca cagctcgggc agaaaccgcg
     3361 acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa gactcgcagc gaacccgtag
     3421 aaagccatgc tccagccgcc cgcattggag aaattcttca aattcccgtt gcacatagcc
     3481 cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat actcgtcaac
     3541 gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag tatcccgatc
     3601 ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa tactgtgttt
     3661 acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg cctgccgttg
     3721 ctagacattg ccagccagtg cccgtcactc ccactcttac attgttcgca gtatctaggt
     3781 acatgttatt gcgtgcacct tcatgatgta taatgttatt aatgacagca gaaagatcga
     3841 cagaattaat attcttcgta cctgggacag ttatcgggtg tcggccagtg atgagaccaa
     3901 aggatgcagt ataagatgaa ccactactat tatcatggcc accattacca ccaccgttaa
     3961 aactacctcc cgaacttcca gagccgctat gaccatcatt gtaggggtca tttggtccca
     4021 tgtgaccaaa accaccgaca gtaccgctat ttaactctgt actcataaaa ttatcctctt
     4081 tgacttttaa aaacagcaaa aactgtattt ataacaatgt gtttatatac agtattcgaa
     4141 atactacgcc cgtcaaaacc acaccgtcaa caggtttttt gtgtattttt cacgcctcac
     4201 gggcagcctc agaccttttg tgttaaaaca gtaatatgtt ctgctgttac aatacggtac
     4261 acacaggaga cactgtcact tttatggcat ggcacacagt tcaagagaca ctgaaattaa
     4321 caggaaagtc gcgctcgcaa ttatatcgtg acatggctaa aggccttgtc agctatcgca
     4381 caggaaaaaa tgatcgccgg gagttcgaga ccagtgaact gattcgggcc tatggcgagc
     4441 tgcgcacttc tgagacacca aaaaggcaca aggagggaca ctccaaatct gtcgttagcc
     4501 aggcggaaaa tgctcagcta gacgtcattc agcagaaact ggacgctctg cagcagactg
     4561 ttaccctaat gcttgaagac aagatggcgc gggaagcaga aaaccgcctg cgaaatgagg
     4621 agagggaaca actacaggcc gaaattagcc gtctggctga cgcgctggca caggaaaaaa
     4681 agcggggatt ctggtcgcgg ctgtttcgct ccggttcaga gtaacagtgc aggtgactct
     4741 gatttaccat aacatctgtt gtacgcaccg ctgaaatgcg ttcagcgcga tcacggct
//
LOCUS       DAJTMK010000473         4797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella dysenteriae isolate 403751
            SAMEA8900129-rid15820803.guided.248, whole genome shotgun sequence.
ACCESSION   DAJTMK010000473 DAJTMK010000000
VERSION     DAJTMK010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900129
            Sequence Read Archive: ERR6005892
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella dysenteriae
  ORGANISM  Shigella dysenteriae
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478806.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478806.1
            Genome Coverage       :: 175x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:19:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,562
            CDSs (total)                      :: 4,479
            Genes (coding)                    :: 4,082
            CDSs (with protein)               :: 4,082
            Genes (RNA)                       :: 83
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 72
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 397
            CDSs (without protein)            :: 397
            Pseudo Genes (ambiguous residues) :: 0 of 397
            Pseudo Genes (frameshifted)       :: 203 of 397
            Pseudo Genes (incomplete)         :: 110 of 397
            Pseudo Genes (internal stop)      :: 126 of 397
            Pseudo Genes (multiple problems)  :: 41 of 397
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Shigella dysenteriae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900129-rid15820803.guided.248"
                     /serovar="S. dysenteriae provisional 96-3162"
                     /isolate="403751"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:622"
                     /country="Kenya"
                     /collection_date="2010-12-14"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OO135_004557"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OO135_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5400074.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1527..2285
                     /locus_tag="OO135_004558"
     CDS             1527..2285
                     /locus_tag="OO135_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5400075.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2285..3121
                     /locus_tag="OO135_004559"
     CDS             2285..3121
                     /locus_tag="OO135_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5400076.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSSAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3228..3632)
                     /locus_tag="OO135_004560"
                     /pseudo
     CDS             complement(3228..3632)
                     /locus_tag="OO135_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3676..4065)
                     /locus_tag="OO135_004561"
     CDS             complement(3676..4065)
                     /locus_tag="OO135_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5400077.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4283..4723
                     /locus_tag="OO135_004562"
     CDS             4283..4723
                     /locus_tag="OO135_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5400078.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1123 a         1299 c         1255 g         1120 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct ggtgattttg
     1561 tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg
     1621 agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg
     1681 aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg gcaattccgg
     1741 gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg tcaatggggc
     1801 agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt
     1861 cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct
     1921 taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc gaacgagagc
     1981 taccggtgcg gctcgaccaa gagcgcaccg atatggttgt ttgccatggt gatccctgca
     2041 tgccgaactt catggtggac cctaaaactc ttcaatgcac gggtctgatc gaccttgggc
     2101 ggctcggaac agcagatcgc tatgccgatt tggcactcat gattgctaac gccgaagaga
     2161 actgggcagc gccagatgaa gcagagcgcg ccttcgctgt cctattcaat gtattgggga
     2221 tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg attggaccct ctgacttggg
     2281 gttgatgttc atgccgcctg tttttcctgc tcattggcac gtttcgcaac ctgttctcat
     2341 tgcggacacc ttttccagcc tcgtttggaa agtttcattg ccagacggga ctcctgcaat
     2401 cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg ccgactatct
     2461 ggtatggcgc aatgggaggg gagcagtccg gttgctcggt cgtgagaaca atctgatgtt
     2521 gctcgaatat gccggggagc gaatgctctc tcacatcgtt gccgagcacg gcgactacca
     2581 ggcgaccgaa attgcagcgg aactaatggc gaagctgtat gccgcatctg aggaacccct
     2641 gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga
     2701 tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag ccgatcaaat
     2761 gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg aaaacatcat
     2821 gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg aagtgggctt
     2881 tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc tcgatcctag
     2941 acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct
     3001 gctcgaccag gcgtacgctt atgggtgcct ttcctcagct tggaacgcgg atggagaaga
     3061 ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta
     3121 gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct
     3181 ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa caggaaacgc
     3241 agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag tacttcatca
     3301 accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca gaaaccgcga
     3361 cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg aacccgtaga
     3421 aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg cacatagccc
     3481 ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg
     3541 atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc
     3601 cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta
     3661 catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc
     3721 tagacattgc cagccagtgc ccgtcactcc cactcttaca ttgttcgcag tatctaggta
     3781 catgttattg cgtgcacctt catgatgtat aatgttatta atgacagcag aaagatcgac
     3841 agaattaata ttcttcgtac ctgggacagt tatcgggtgt cggccagtga tgagaccaaa
     3901 ggatgcagta taagatgaac cactactatt atcatggcca ccattaccac caccgttaaa
     3961 actacctccc gaacttccag agccgctatg accatcattg taggggtcat ttggtcccat
     4021 gtgaccaaaa ccaccgacag taccgctatt taactctgta ctcataaaat tatcctcttt
     4081 gacttttaaa aacagcaaaa actgtattta taacaatgtg tttatataca gtattcgaaa
     4141 tactacgccc gtcaaaacca caccgtcaac aggttttttg tgtatttttc acgcctcacg
     4201 ggcagcctca gaccttttgt gttaaaacag taatatgttc tgctgttaca atacggtaca
     4261 cacaggagac actgtcactt ttatggcatg gcacacagtt caagagacac tgaaattaac
     4321 aggaaagtcg cgctcgcaat tatatcgtga catggctaaa ggccttgtca gctatcgcac
     4381 aggaaaaaat gatcgccggg agttcgagac cagtgaactg attcgggcct atggcgagct
     4441 gcgcacttct gagacaccaa aaaggcacaa ggagggacac tccaaatctg tcgttagcca
     4501 ggcggaaaat gctcagctag acgtcattca gcagaaactg gacgctctgc agcagactgt
     4561 taccctaatg cttgaagaca agatggcgcg ggaagcagaa aaccgcctgc gaaatgagga
     4621 gagggaacaa ctacaggccg aaattagccg tctggctgac gcgctggcac aggaaaaaaa
     4681 gcggggattc tggtcgcggc tgtttcgctc cggttcagag taacagtgca ggtgactctg
     4741 atttaccata acatctgttg tacgcaccgc tgaaatgcgt tcagcgcgat cacggct
//
